LOCUS       DAOSVE010000001        50362 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_476_length_50362_cov_69.459479, whole genome shotgun sequence.
ACCESSION   DAOSVE010000001 DAOSVE010000000
VERSION     DAOSVE010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 50362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50362
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_476_length_50362_cov_69.459479"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PK016_00005"
     CDS             complement(<1..238)
                     /locus_tag="PK016_00005"
                     /inference="COORDINATES: protein motif:HMM:NF024768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transporter RnfC"
                     /protein_id="HXL01494.1"
                     /translation="MKLKTFPGGIHPPTFKEFTATKTIKEFPLPSRVVLPLLQHTGAP
                     LSPLVKKGDKVKRGQKIADVEAFISAPLHSPLAGK"
     gene            complement(262..1608)
                     /locus_tag="PK016_00010"
     CDS             complement(262..1608)
                     /locus_tag="PK016_00010"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HXL01495.1"
                     /translation="MFNKVLIANRGEIALRVVRACRELGIKTVGVFSDPDYNLLHLKF
                     VDQKVALGGSLPQDTYLNEEKIIRACELTETSALHPGYGFLSENAEFARKLKEKGITF
                     IGPDADLLERMKNKLQAKRILEEAGIPVVPGSLEPIKDEKEAKEIAEKIGYPLFLKAS
                     RGGGGRGIRVVREPHQLAEALESAKREGKLAFGSEEVYMEKLLTKARHVEVQIVRDKE
                     GNVSHLGERECSLQRRQQKILEEAPAPFLTEETREKLRETAIKVAEVLNYTTCGTVEF
                     LVTEEGSFYFMELNARIQVEHPVTEMISGIDLVREQIGLAAGEKLSFSPEEIFLRGHA
                     LEMRINAEDVNHNFSPAPGKISRFDVPRGPGIRVDSHCYSGYEVPPYYDSLLAKLVVW
                     DCDRDFAIRRAREALHSFMVEGILTTIPFHMKILQNQDFRVGNLHTRFLEENLEEY"
     gene            complement(1620..2981)
                     /locus_tag="PK016_00015"
     CDS             complement(1620..2981)
                     /locus_tag="PK016_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HXL01496.1"
                     /translation="MLLSESRELLSLWEKVQKKAANFSPATLILLGFVIIILSGALLL
                     LLPSMVNPGNKLRFIDALFTATSATCVTGLIVVDTATFFSFWGQLVILALIQIGGLGY
                     MTLNTLLALALRRRIEYRTRLAIRDSFSLDFPGGVVRFVLSVLKYTFFIEGIGSLVLF
                     LNFLRYFTPAKALFPAIFHSISAFCNAGFSIFSNNLESFVLDPVVSFTVMGLIILGGL
                     GFVVLREIVEKRSVSSLHSKVVVRTTLFLISLGTVLILLLEWNNPATLAELPWGGKVM
                     ASLFQAVTPRTAGFNTIPIADLREASTFLIILLMFIGASPGGTGGGVKTTTFALLLGT
                     AVSVIKGEDRVEFFHRRVIPGAVFRAMSVVVLAFLLIFGVIMILLVVQPASLLDICFE
                     VVSAFGTVGLSRGITPNLLDLSKILIIVTMYVGRVGIMTLIMVRPSRGKDLITYPEDR
                     VLI"
     gene            complement(2999..4126)
                     /locus_tag="PK016_00020"
     CDS             complement(2999..4126)
                     /locus_tag="PK016_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXL01497.1"
                     /translation="MLGRERALRALHHQVPDRIPLDLGSTNCTTMTKVAYDNLKRYLG
                     IEKETKFMMENFQIVFIDEEVLQILGIDTRGIHPRPVFFKNMVDERTYFNEFGIKYHM
                     PEDGLYYDMVEHPLAGKSIEELQDYEWPDPAKTMDLSGVREKAKNLKESNEYLLVGDM
                     IDTGIFEPCWYLRGFENFLVDLLTDVDFVTTLLEGMYTYQLKKYSLFLQEVGEFLDVV
                     FVGDDLATAESVIMSPKTYREIIKPYHKEYFKNLKKMTPQAQFLYHSCGSIFSFIPDL
                     IEVGVDILNPVQVSAQNMDTKVLKEKFGGELSFWGAIDTTQVLPQGAKEEVREEVRKR
                     IDDLGPEGYVLTSVHDIQPDVPPENIMTMFEEAKEYGAFSL"
     gene            4281..4790
                     /locus_tag="PK016_00025"
     CDS             4281..4790
                     /locus_tag="PK016_00025"
                     /inference="COORDINATES: protein motif:HMM:NF019099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HXL01498.1"
                     /translation="MKTNTNAANIARLGILVSLGIVLHIVESLLPSFFLPGAKLGLAN
                     VVTLVILWRGNVVEAIEVAILRIVLGNILSGNLGIGFFLSLGGGLASLGGMSFFKKRG
                     VSLWGVSIMGALFHNSGQLLVAYLYMKSENIFYYLPFLVLFSIPTGLLIGYLGQCLLH
                     WEEKIFSLS"
     gene            4871..5785
                     /gene="ilvE"
                     /locus_tag="PK016_00030"
     CDS             4871..5785
                     /gene="ilvE"
                     /locus_tag="PK016_00030"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HXL01499.1"
                     /translation="MQELEKIWMNGELVPWKEAKIHVLTHALHYGTAVFEGIRCYKTN
                     TGPAVFRLKDHLKRLYDSAHIVRMTIPYPPEELEKGVIDIIKANGVEECYIRPIVFRG
                     YGEMGVDPSSCGVDCVIAVWCWGAYMGEKGIKEGVRAKISSYTRTYINSGSPRAKSSA
                     NYLNSSLAKIEAKDLGYDEAILLDINGFISEGSGENVFYVKDGVIFTPHPYSILLGIT
                     RDTVIKIARKLGMEVKETLCTRDDLYLADEAFFTGTAAEITPIAEVDGRKIGTGKRGE
                     ITHLLQEVFFRVVRGEEPDYESWLTKLR"
     gene            5841..6140
                     /locus_tag="PK016_00035"
     CDS             5841..6140
                     /locus_tag="PK016_00035"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXL01500.1"
                     /translation="MRAIKEEAVSTIEGFNHFYGKVMNEGSLSLKVKELIALAVGVAV
                     HCENCIMIHTRGAKKSGATREEILEAASVGVVMGGGPAFTFLPLVHRTLDSLEKE"
     gene            6160..6972
                     /locus_tag="PK016_00040"
     CDS             6160..6972
                     /locus_tag="PK016_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012058091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HXL01501.1"
                     /translation="MPYIAFLTDWGYKSYYVGVTKAVIKKINPQVEIIDLTHEVEAFN
                     VREAMYILYRAFRDFPPESVFLAVVDYGVGTERKALALQVEKGYTFVGPDNGLFTLIV
                     ENEKVVKIVELNRTQYFYHPLPSSTFQGRDIFAPCAAHLSRGIPIEELGSSVDFKDLI
                     LLPYEKPEIKNHCLHGEIAYADRFGNLQTNLPGYYFQEIEIKEGGKLTIEVGEKKFIA
                     TYHPVYAFASKGELLIHPDSSGYLEIAAYQESAQAIIKARGGEKILLHKIVS"
     gene            complement(6969..8495)
                     /gene="xylB"
                     /locus_tag="PK016_00045"
     CDS             complement(6969..8495)
                     /gene="xylB"
                     /locus_tag="PK016_00045"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006772354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HXL01502.1"
                     /translation="MGKLIIAHDLGTTGNKATLFNEEGEKIASEFFGYDTYFPDALSV
                     EQDAEDYWQAVVYSTRQLLEKGKVKKEDVEVISFSGQMMGALPVDEKGQPLYHILIWA
                     DRRGIEEVEWIKERIGEDRIYQLTGHRLSANYSLAKILWFRNHLPEVYNKAYKFLLAK
                     DFVVFRLTGQWATDFSDASGTNLFDLARETWSGEILEKVGIEEDKLPPLYPSTTVVGE
                     LTKEAGEECGLLPGTPVVIGGGDGPCAACGAGIVEKGEAYLYLGSSSWIAFASSQPFY
                     DPEKRTFTFHHLEKGLFMPTGTMQSAGGSYQWCRDAVCEREKVLAQELGVSAYQLMDL
                     EASKVSPGCGGLLFLPYLLGERAPWWNPHARGVFIGLTPSHNKSHLIRSVLEGVSLNL
                     RIILDAFREQGLCPDTLRLIGGGAKGDFWSHILAQVLGVEILRPRYLEEATSLGAAIA
                     GGVGAGIFKGLEVAKELVKIEDHFSPRAEMVEVYNRLYPIFKKAYLSLLPVFDDLSSF
                     "
     gene            complement(8470..9795)
                     /locus_tag="PK016_00050"
     CDS             complement(8470..9795)
                     /locus_tag="PK016_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HXL01503.1"
                     /translation="MGYLATILLILLLIFINAFFAASEIALISTRRVKLRLMKEEDSQ
                     ELEAIQQFLEKPNEFLATIQICITLAGFFASATGAVSLSRELGRRLESTSLPILGTLG
                     EELAVILITFIISYLSLVLGELVPKRVALANPEKFALRIARPIVFLSRAFKPLVTLLS
                     VSTDFISRILGVPQESVYSVTDEELKLFIAEQRSLPLDERRLINEVFDFGDTLAYEVM
                     TPRTDMVCIEDNATIKDALELSLQSHFSRLPVYRGDLDNILGFVHIKDILPHLNTGDM
                     DKPVREIMRPIHFVPLTKKAMELLKELQQKRINMAIVLDEYGGTAGLVTIEDLLEELV
                     GEIRDEHDRETAPYRKIGDNEYLVDASLPIESLNELLGLLLPESEEFESVGGLVMELL
                     GKVPEEGEMVEVNSYQLKVERMKGKRILTLRLLVKKKKEGENGEANYSS"
     gene            complement(10029..11228)
                     /locus_tag="PK016_00055"
     CDS             complement(10029..11228)
                     /locus_tag="PK016_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HXL01504.1"
                     /translation="MPDWKALLDNEFDLFEPINLAQNLVRIPSVSGEGGEVELARFIA
                     NYFIDRQIKVEWQEVEEGRANLIAEVEGILGEGPNLLFNGHLDTVPLGSGWTYPPLGG
                     EIIENRLYGRGACDMKGALASMMYTAYMVSLFAPSLYGKLKLVLVVDEEGSNLGVKHW
                     IKSYKERGEMIDFALVGEPTGLNISLGHRGVAAYQLKVQGKSCHMGIAERGINAIYLA
                     SRIIQSIEAKNQELKKFTDPDLGNPALHVGKIQGGTFPNVVPDICSFEVDVRTVPDFP
                     LEKMEETLQNILDDVQQEKELKFNYEIDQSIPYLPPVKISRDLEGVRILSRSISDVPG
                     EIPTFAPFPASCEASFLVEAGIPTLIFGPGRIEEAHTANEFISVTQIVAASRIYSLLA
                     LRFLGGE"
     gene            complement(11239..11814)
                     /gene="pyrE"
                     /locus_tag="PK016_00060"
     CDS             complement(11239..11814)
                     /gene="pyrE"
                     /locus_tag="PK016_00060"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HXL01505.1"
                     /translation="MEKEEILEIFRQAGAFLEGHFLLTSGLHSPFYIEKFKILQFPRY
                     VEILCGEIIRHFSGEKIDVVVGPAVGGIILAYEVARQLGVKMAFTEREEGKMVFRRDF
                     QLKKGDRVLIVEDVVTTGASVEEVIKRVEETGSQIVGIGVLVDRSGKKASFSYPFYPL
                     LEIEAQTYSPEECPLCQQKIALQKRGSRYLS"
     gene            complement(11819..12727)
                     /locus_tag="PK016_00065"
     CDS             complement(11819..12727)
                     /locus_tag="PK016_00065"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HXL01506.1"
                     /translation="MVNLAVNLGGLNLKNPVILASGTCGYGREIAPYLDLNLVGAITV
                     KGLSYYSFPGNPPPRLREVYAGILNSIGLENKGVHRFIREDLPFLSSFDTRILVNIWG
                     ETADEFVQVAQELDKFERVDALELNVSCPNTERGGAVFCSDLEMLKSIVGRVRNATQK
                     PLIVKLGPEVKEWGKTLDILESEGADILSLTNSFPALFIDVENMNFFFKRKFAGFSGP
                     AIKPLALKMIYEVVNSTSLPVIGMGGIVTAYDALEFLLVGAKAVALGVANLLEPSSAL
                     SILEGIKQYLEDRKIENLEDLIGRVK"
     gene            complement(12721..13491)
                     /locus_tag="PK016_00070"
     CDS             complement(12721..13491)
                     /locus_tag="PK016_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HXL01507.1"
                     /translation="MKKAIIEEKKCWRERFIQLKLKEKEIAYCAQPGQFVMVRVQEGF
                     DPLLRRPLAIMKVEGDSFYLFFEVVGRGTKILSSLNVGSRLEVLGPLGKGFSLSPSQA
                     LIVAGGRGITPLYFLASHFKENKIPFIFFLGVKNKKELFMVNFLQAFSGACFWSCEEP
                     IEDGFCGTVLSLFRTWLEDKALSTGTRVYACGPEGMLRNLYQEEGIQNIPLEVSLETF
                     MGCGFGVCMSCAVEKGDGEGYFHVCKDGPVFLAQELKW"
     gene            complement(13488..14408)
                     /gene="pyrF"
                     /locus_tag="PK016_00075"
     CDS             complement(13488..14408)
                     /gene="pyrF"
                     /locus_tag="PK016_00075"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HXL01508.1"
                     /translation="MNFADQAIKKTQEKGPLVVGLDPHFQFLPPYLWEKWVEKEGKTW
                     EALSHCVLEFNSLILQALTSVAGFAKIQVAFYEALGVPGMIALKETIDEARRKDFIVI
                     LDGKRNDVASTATFYAQGYLSQLEIGKRLTLPSFWNVDAITVSPYLGEDSLQPFFEEA
                     KKEDKGVFVLARTTNPSAPFIQDVGEEKVFVRVARLVEKWGEEVMGESGFSSLGIVIG
                     ATYPEDMRFLREEFPSLLFLVPGIGAQGGDFSSLRFAFRKDGEGALITISRDIIFYYQ
                     KEKNDKGWGFEEKAKERAIFYQERLREILK"
     gene            complement(14395..15699)
                     /locus_tag="PK016_00080"
     CDS             complement(14395..15699)
                     /locus_tag="PK016_00080"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HXL01509.1"
                     /translation="MKKILIKGGTLVFPQKGVFQDADILLENGLITRMGKGIEDAQAF
                     LINASNLLVGPGFINLHVHLREPGRESEENLLTGSRAAARGGFTSVVSMPNTHPPIDN
                     PVLVRYLLLRAKEVGLVNIFPAGAITVGLEGREMAEYGLLKEAGVVALSDDGKGVQDA
                     HLLYLAFQYAQYFHLPFILHEEDETLSRDGEISEGKTSIVLGKRAIPRVAEESLMARD
                     LVLAYRTGARVHFTHLSTSLEVELLRWFQKKMPISADVTPHHLLLTEDDVLGLGNEAK
                     VKPPLRSLEDQNVLKEGIKEGLISILASDHAPHPSEDRNRSFSEAPFGISNLEITVPL
                     YAKALIEERVINWVELWEKLSYNPALFLGLVNKGILEEGKDADITIVDPETKKVVEVS
                     NFESRGKNCPFENWSLSGWPLYTIVGGKIVMVEGSVVESEFC"
     gene            complement(15696..16622)
                     /locus_tag="PK016_00085"
     CDS             complement(15696..16622)
                     /locus_tag="PK016_00085"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HXL01510.1"
                     /translation="MKWKYKDLLGINLLTGKEIEYLLERGNYFREFLNCSPKKMKYGE
                     GYCLVNFFLEPSTRTRVSFEVAGRLSGMEVVNFTTEISSFQKGESLEDTAKTLSRMKF
                     DVLVVRHRQSGIAEYLAQLLPLHVINAGDGMREHPTQALLDLLTLQRKFGYFQGLKVA
                     IVGDVLHSRVARSLALGLKKLGAEVFLSGPPTLLPPTMENLGAKVVFPVEEAVEGVDV
                     IYLLRIQKERQEAGYFPSIREYAHFFGLKEKWLDRENGRPFIMHPGPVNRGVEISYSL
                     AEEDFSLIEEQVTCGVAIRMAVLETLLQGEQQ"
     gene            complement(17131..17988)
                     /locus_tag="PK016_00090"
     CDS             complement(17131..17988)
                     /locus_tag="PK016_00090"
                     /inference="COORDINATES: protein motif:HMM:NF001989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HXL01511.1"
                     /translation="MKNRSLMVQGTGSGVGKSVIVAGLVRLFARQGIKVAPFKAQNMS
                     CNSGITEGGEEIALAQILQAQAAFQKPDSRMNPIFLKPQGNGKSEIIIRGKTWAMGKV
                     SSYYQDKEFFWEVIKESLDSLGEEYDLLILEGAGSPAEINLRGKDMANMKVALYLQSP
                     VMVVGDIERGGVFASLYGTWALAKEERELIRAFIINKFRGDAEILKPGLREIEELTKV
                     PVVGVLPYVSLSLVEEDSLLEKPGGKDCFSCSGGDFSLGEVDQELDWWSKILKRHLNI
                     KKIEEMIGL"
     gene            complement(17985..19229)
                     /locus_tag="PK016_00095"
     CDS             complement(17985..19229)
                     /locus_tag="PK016_00095"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL01512.1"
                     /translation="MLSRRDYFFNFAVDALDYAFFSLGMSFGSTVTLLPLFAQRLGAS
                     NLEIGLIPAIAYLGWSFPAIWGARVSGKLERKLPFILKFTLFERLPFLGMALVAFYLV
                     PYYPSLSLYLMFGLLGINSFAMGFLGPIWTEMIGKVIHNSRWGLYFACGNGVGALMGM
                     WGSTLAEKFLKTYSFSHNFGYCFLLASLSMVVSYFFLALTREEKEPETSSSVEKFLPS
                     FFRVMKEDANFLSFMLARVFLALGVVGGAFYTVYTLQKFQIPDALVPRYNAVLLVSQA
                     LSNFIWGPLGDKKGHKLVVLLGATSMVLSNLLALFSPDYRWFFLAFALLGFNYSAISV
                     GGTAIVLDFAPRERRGLYLGWSSFLSNFPAFFSPLIGGKIADWFGYYWVFWLALILNV
                     IGLLWILWGVKEPRVFSNFEEI"
     gene            complement(19229..21181)
                     /locus_tag="PK016_00100"
     CDS             complement(19229..21181)
                     /locus_tag="PK016_00100"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HXL01513.1"
                     /translation="MKGILRIDYEEELNEEQKKVVFASYGPILVIAGAGSGKTRTITY
                     RVARLVEEGVDPSSLLLATFTNKAAREMLHRVECLLGISLEGMWGGTFHHIGNLILRR
                     EAKKIGYDNNYTILDREDQKDLVEATIEEMGIDRRSRRFPKGDVIANIISLSRNTFIS
                     VEDILEKFYPQFLEWVSQISSLFALYQDRKRQLSLMDYDDLLYYTWSLLQEDENTRRK
                     YGNVFHHILVDEYQDTNLLQAEIVDLLAREHRNLLVVGDDAQSIYSFRGANFVNIMRF
                     PERYPGCQIFKLETNYRSTAPILDLANQVISFNFHQFPKVLHPVRKGGEKPVVVPLRD
                     VMEQAEFVASQILELHNEGHDLREIAVLYRAHYHSMEIQMELTRRGIPFEIRSGLRFF
                     EQAHIKDVVAYLKVIANPRDEMAWKRVLKLYPGIGRASAEKVWEALSSSPDPLTTLER
                     EEIFSLLPVNARVSLASLRDLLFRLEDLDSSPSTLIREVLEGGYRDYLITHYPNYEER
                     EEDLEELANFALRYKSLETFLSELALLGEIEAENVVRGGIEDERVILSTIHQAKGLEW
                     DCVFVVWLCEGGFPSPRSLGKEEDIEEERRLFYVACTRAREHLFLCYPILAERNRTRS
                     MVRKPSRFLQELDPRSYIKWRGGRRE"
     gene            complement(21194..22084)
                     /gene="hslO"
                     /locus_tag="PK016_00105"
     CDS             complement(21194..22084)
                     /gene="hslO"
                     /locus_tag="PK016_00105"
                     /inference="COORDINATES: protein motif:HMM:NF001033.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HXL01514.1"
                     /translation="MGNYEDKVVCTIVEDTTVVVYVASTTSLVEEARFLHNTSPVATA
                     ALGRALTLAGIMGAALKEGQKVTLYIRCLGPLESILVQANSQGEVRGYVSQPLVFIPS
                     REDKKLNVEEAVGKGSQLSVVKDLGLGEPYAGYIEMPRGGIAYDLAYYFALSEQLPSA
                     CSAGVYVGEKGEVLSAGGFIIHTPAGTPSAVVKKIEDNISVLPPVSSLLFEGKSPEEI
                     IQNLLASLSYKIVGEKKLHYHCYCSRERARRTLILLGKEELEDLLAQEGRAEVSCPFC
                     NRVYLFGDRELKELIEKGNK"
     gene            complement(22085..22708)
                     /locus_tag="PK016_00110"
     CDS             complement(22085..22708)
                     /locus_tag="PK016_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01515.1"
                     /translation="MFLDYWLLEIEKIGEGWLIFLLLGGGIISGFYFFRLKKILFPLL
                     TFVVVLWGGGITRGIEKSNLETLSFSDGKVNFFVFLFLALFLAGVAASKKGEKWGRFF
                     SGFWLAANLYYLLIELSSSVELMRFLQHLSFREVVLFSLISGFLFGNKKVLNLFSSLV
                     GSSLLALAYLQVLSKWGGSDTFWDSSPWVLFLFLVFALWMMAVKESD"
     gene            complement(22711..23481)
                     /gene="udp"
                     /locus_tag="PK016_00115"
     CDS             complement(22711..23481)
                     /gene="udp"
                     /locus_tag="PK016_00115"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009836794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HXL01516.1"
                     /translation="MGYYAYHLALKEEEVRGIKLALLPGAPERCCKLASAFGSFEELA
                     FHREFKTVKAENESGKILVVSTGIGGSSLSIAVEELAQLGVNLFLRVGTCGAIQEGIA
                     VGDLVISEGAVRMDGASDHYAPLPYPACAHWEMVGALKKACELLKYRYHLGITCSSAT
                     FYPGQERYDTFTGYIISSLRGSREEWKRLGVLNYEMEVATLFTVARVLGLRSGAICGV
                     LVNRNEKEEVEEEKIEEIEDRLSEVVAKAAQLILSGEE"
     gene            23537..25159
                     /locus_tag="PK016_00120"
     CDS             23537..25159
                     /locus_tag="PK016_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01517.1"
                     /translation="MPKKICYLYWEKEKIPVPRGKTLQEVVKKIFPSWERIMAAQLEG
                     EIVDLNTEVERDSKVKFFDFSHPEGRRTYLRSLLFVLSYSVEKIFPGHRLKVLYSLGE
                     GLICEIEGVNISSEQLEQIEEEIKNTIAEDLPFTREVVDWEKATAIYQKQGRKDLVKL
                     FRYWRTPAVTVYRLKNFYDHYYGPLVPSTGYLKNFGFVLVPPQFVIQLPTGEDLQRLP
                     TYTFRPKLFSTFQEAVRWAEILAIENVGELNEAIARGEGREIISIAEALQEKKIAQIA
                     DLITQKKGTVHLVLIAGPSSSGKTTFARRLYIQLRVNGWQPITISLDDYFLSREEISE
                     EDYEKPEALDLKLFEEQIKALIRGEEVEIPRYNFITGSREPRGSKTQLSPDGIIIVEG
                     LHALNPRLGENIPGGEKFKIYVSAITQINVDDHNRISTTDCRLIRRLVRDSQFRGSRG
                     EGVFEDWPRVREGEEKYIFPYQENADIMFNSSLIYELSILRQYAEPLLRAITPESPHY
                     LEAYRLYAFLNHFMPLNSSFVPSNSILREFTG"
     gene            complement(25156..26700)
                     /locus_tag="PK016_00125"
     CDS             complement(25156..26700)
                     /locus_tag="PK016_00125"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXL01518.1"
                     /translation="MQLTFLGATREVTGSCYLLKTGDKSFLVDCGMVQGRGEERNYQP
                     FPFSPGDIDFILLTHAHLDHSGRIPLLFRNGFRGKIYATLPTIELSHLLWLDTVKIMG
                     EETERINRKNIRQGKPRVQPLFGEEEVEGAMNLFVPVGYDELINEGEIEFVFRNSAHI
                     LGGAALEVWGEGSKVVFSGDLGQFYSVMEGSPPVIERADFVVIESTYGNRNHRSLEDT
                     RREFTEAIDGALSKGGKVLIPSFVVDRAQRVIYELSLLPEKVRNKYPVFFDSPLGAKV
                     TDVYERYRNLLSGEIQKLNWEKKGPFSLPNLRYVSTSTESKAINNINQAIVIAGSGMC
                     TGGRIVHHLKHNLYKENTTLIFVGFQAQGTLGRALIEGAKKVRVLGEEIAVRAKIYTI
                     NGFSVHADQEDLFKWVEYFQGSPTFLVTHGEESIAESFARSLKEKGKEAIVPHLGDTI
                     DLGKKKVFSAPSLPPEENILEKLESKVSSLQSIYPALGEEEKILLRSALILLDEIEKR
                     KGESLV"
     gene            complement(26713..27591)
                     /gene="galU"
                     /locus_tag="PK016_00130"
     CDS             complement(26713..27591)
                     /gene="galU"
                     /locus_tag="PK016_00130"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HXL01519.1"
                     /translation="MKEVRKAVIPVAGLGTRLLPATKTQPKEMLPIVDKPAVQYVVEE
                     AVASGIESILFVTGRGKRAIEDYFDYSVELEQELENRGKEELLKEIKKVGNLVPIYYV
                     RQKSPRGLGDAVYQAREFVGEEPFALLLADDIFLTPSPFLEEIIELYHQKPAVYLTVT
                     PVPPQDVSSWGVIKGEKVKENLYRVEDLVEKPEKDKAPSSLAIVGRYILPPSIFETIR
                     CTLPGRGGEIQLTDAIRALLGKEEIYALELRGKHFGVGDKVGYLKANIAFALEREDVG
                     GEIKAFLREILQEENR"
     gene            complement(27588..27890)
                     /locus_tag="PK016_00135"
     CDS             complement(27588..27890)
                     /locus_tag="PK016_00135"
                     /inference="COORDINATES: protein motif:HMM:NF036410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 family protein"
                     /protein_id="HXL01520.1"
                     /translation="MWGAFLNFLVMALNGGKMPVSLEVTSWLGLQQFVELLENNYYPD
                     YTIISSSTRLPFLGDMLPYFSFLFRRFFVASVGDYLLGIGVMLFLVHHLRKEEAKT"
     gene            complement(27999..28553)
                     /locus_tag="PK016_00140"
     CDS             complement(27999..28553)
                     /locus_tag="PK016_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01521.1"
                     /translation="MIKVGDWKTVEEAIGSIEGVINVKVVGEEEIKEIHVLSKPSKPP
                     KLLARDIETLLQARFNLEIDHKKISIVAFDLEEEKKESGLRPVLWGLSWKKNPQTFQV
                     EVEIKLSDKLYRGMVTGSVSNSRNCYSLVVQATLECLNQRIGNVVFAPRGVLIQRLGD
                     LEVALAFVDCNIPQREEMLVGQLW"
     gene            complement(28563..29099)
                     /gene="raiA"
                     /locus_tag="PK016_00145"
     CDS             complement(28563..29099)
                     /gene="raiA"
                     /locus_tag="PK016_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HXL01522.1"
                     /translation="MNIVVRGKNIEISETMRNYVTKKLSKLERFFDRLMDATVNFTLE
                     RGRIKVESTLTASGIVIRGEAVGSDWRSALDEVMDKLERQVKRYKERLGKRGVLRKEA
                     VVEFEREEVEEPALPPVDKVARVKEFVLRPMSIEDAILQMELLGHTFFIFKDLDKDKV
                     QVVYKREDGNYGLIDPIY"
     gene            complement(29062..30051)
                     /locus_tag="PK016_00150"
     CDS             complement(29062..30051)
                     /locus_tag="PK016_00150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HXL01523.1"
                     /translation="MIFVLCANPALDRLLIMKEINLNEVNRASQVVETAGGKGINVAR
                     AIKRKGGNPHLLLFEGGSTGERIKNSLEEEGFLLTNWPSGETRVTTVIHEEKTERHTV
                     INEPGPLVSVSAVLTFLRFLENNLQKGDCLVLSGSLPRGVRLDFYALLTGVAKRKGAL
                     SVVDTSGEPLRRALSASPFMIKPNARETEEVLGFSISSWEDKLKAVKIFQNQGVELVI
                     LSDGKRGVVAAYQGEIWKVRMRKETEGGGYSIGSGDTLVGVMVKYLEEGRNIEGSLAA
                     AVSCGLANTFYPGGGIFDLEKAEELEKNVLVERIKSQEAAGDEHSGARKKHRN"
     gene            complement(30048..31031)
                     /locus_tag="PK016_00155"
     CDS             complement(30048..31031)
                     /locus_tag="PK016_00155"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HXL01524.1"
                     /translation="MDKKAVCLGELLIDFVSTVNGVTLKDAPGFEKAPGGAPANVAVG
                     LAKLGIDTYFIGKVGKDAFGEFLREVLERNGVNTRFLFSTEKAKTTLAFVSLTQEGER
                     DFVFYRDPGADMLLDQREIGEECFSGKGVFHFGSITMTHEPAYSATMRAISLAKKYGY
                     LLSFDPNLRPALWRDLEEARFRMRQALKSVDILKINEEEARFLAGTSDLEKALEFISQ
                     NFSLALVAVTLGKRGCFIVQGERRLEMEGFSVATVDTTGAGDGFVAGLLSSLYMFWED
                     LRERKEIPESAIAHAARRANAVGALTTTKKGAIPALPTSEEVEIFLKEHPL"
     gene            complement(31049..32572)
                     /gene="xylB"
                     /locus_tag="PK016_00160"
     CDS             complement(31049..32572)
                     /gene="xylB"
                     /locus_tag="PK016_00160"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HXL01525.1"
                     /translation="MEYFLGIDVGTTGCKVVLVNEEGELVSKAVEEYPLYTPYPNWAE
                     QDPADWWRGTVQAVKKALQAAEINPHLVKGVGLTGQMHGSVFLDEKGEVLRPAILWCD
                     QRTARECEEITRIVGEEKLMSINCNPVLPGFTAPKILWLKNNEPHLYERVAKVLLPKD
                     YIRFLLTGDFATDVSDASGTSLFDVPRRKWSEEIISLLGFPPSWFPPSFESPEITGYL
                     KKEVAEMWGMGDEVLVVAGGGDNAAGAIGTGIVKPGLVSASLGTSGVVFAFSDEVKVD
                     KKGRVHTFCHAVPGKWHVMGVMLSAGGSLRWFRDRLGMEEKNLASLLEKDSYDLLTAE
                     AEKALPGAEGLIFLPYLTGERTPHADPYARGVFFGLTLKHRKNEMIRAVMEGVTFGMR
                     DSFEIMREMNIPIQEVRAIGGGARSALWRKIQASIYGVPLFGVKIDEGPAFGAALLGA
                     VGRGVFSSVEEASEKAVKVTESVLPSPELVDYYDRAYRLYRDLYPALRNFYPRLQEL"
     gene            complement(32585..33793)
                     /locus_tag="PK016_00165"
     CDS             complement(32585..33793)
                     /locus_tag="PK016_00165"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HXL01526.1"
                     /translation="MAKKVIMPKLGLTMEEGVINKWLVKEGDRVSRGDVLFEVATDKV
                     NMEVESPAEGVVLKILYPEGATVPITETVAYIGKEGEAIPEEETEKLEEKIETKEEVK
                     VEEKREEAPLTLETERVRVSPLARRLAEEHGIDLSQIRGSGPGGRIVKEDIEKAIEEK
                     EKKLIIEEVKAPREKEHIPLSRMRKIIAQRLTESYQSTPHFFLHQEIYAEELVKMREK
                     ILPRVEKETGLRVSYTDILVKMVAKALEKFPLFRAHLVGEEIEISPEVNVGVAVALEE
                     GLVVPVVKRANQKGISQITQEINTLAQKAKSNQLTPEEISGGVFTVSNLGMWGVDSFT
                     AIINPPEAAILACGAIRKKPFFNGEEIVPAYLWELSLSCDHRLIDGALAAQFMQFLKN
                     LLEEPFTLIV"
     gene            complement(33786..34763)
                     /locus_tag="PK016_00170"
     CDS             complement(33786..34763)
                     /locus_tag="PK016_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01527.1"
                     /translation="MVNPAAGKDIRRLIAYGSVFGNQEKINYTLRLLRGIESVVNSPL
                     EVAYMPDPYDLVEIVKKSFSSPEIIFRCAPIAVFGDETDTLNFTDWAVNEKKVDALVV
                     IGGDGTNRIVAKKSALTPLFSLFGGTNNVFAENIEPTVMGMAVGLFLENDSLREKVVK
                     KSKILKAKPREGGKEEIALIDAVVVEKTLVGARAVWEPELIRLIVVTQSSPLKIGLSS
                     VVGRLASISAEEERGAMVELGEGGKIVRAPLAPGLVGEVKIRKWDFLPLGVSFLLPHQ
                     RGVMALDGEREIVLREGEDWEIRLEDTGPYRVDVEKLMNLVQERSVVNG"
     gene            complement(34783..35571)
                     /locus_tag="PK016_00175"
     CDS             complement(34783..35571)
                     /locus_tag="PK016_00175"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HXL01528.1"
                     /translation="MDPQPLAHKVAIVTGSASGIGKGIALRFAEEGADVCCWDMNLEG
                     AKATVQEIEKVGRKSLAFQVNVTKSEEVKDAAQATFNQFGKIDILVNSAGIIRAHFIT
                     DVPEEVWDNIIAVNLKGTFLCIREVAKYMIPQKSGKIISVSSKSGKKGGLWLGAYSAS
                     KFGIIGLTQSVAMDLAPYKINVNAICPGNVFSTPMWDLLDKEYSKKLGVPPQEVRKIY
                     VEKVPLGRECTVEDVANVAVFLASSYSDYMTGQAVNVTGGQEVH"
     gene            complement(35591..>35785)
                     /locus_tag="PK016_00180"
     CDS             complement(35591..>35785)
                     /locus_tag="PK016_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HXL01529.1"
                     /translation="KEVGVFGVFASHASQYEQAAKLIYSKRVRAKRLITHVLSLEEVV
                     KGIDLVRQGVALKAVVSMEK"
     assembly_gap    35786..35885
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<35886..36457)
                     /locus_tag="PK016_00185"
     CDS             complement(<35886..36457)
                     /locus_tag="PK016_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HXL01530.1"
                     /translation="MKAAFFLGPEKMEIREVEVPRVEEGEVLLRVAACAICGTDVRIF
                     HHGHPHVHPPQTTGHEIAGEIVEVGKGVEGYKVGDKVSVITVISCGRCSYCRRGLQNL
                     CPEQKYIGYDYPGGFAEFLKIPREGVEGGNLLVLPPDADLLEASLVEPLSCVVNGQSY
                     LHIGFGENVLVIGAGPIGCMHVELARSQGAG"
     gene            complement(36460..37971)
                     /locus_tag="PK016_00190"
     CDS             complement(36460..37971)
                     /locus_tag="PK016_00190"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HXL01531.1"
                     /translation="MSYLGVDVGTTGCKVVAFTEGGEIIARSYREYPLYYPQTGWIEL
                     DAEEVYRAVEDSLQEVSQDLRNDPPRSLAISSQGEAVVPIGEKGDCLARSIVTFDQRG
                     DEFVPFWEDKIGRRRFFELTGTSLSGIGTVNKILWWKKYKKDVFEKARYFLCFEDFIM
                     YRLGVEPAISFSLAGRTMMFEVQKEKWSEEILSLAGIDPMRLARPLPSGEVVGEVTSS
                     FAQKMGWKEKVIVATGGHDQPCGALGSGVIKPALAMDATGTVECIAPALGELVLSPVM
                     EENNLCCYHHAFPSLYLTMVYNFTGGALLRWFRDNFAQKEKEIAERENCDVYDLILSR
                     LPSQPTNIWVLPHFTTTGTPYFDTQSGGVIVGLKLETSREEFIKALLEGVSYEMKFNL
                     ELLSQAGVKVERLRAVGGGAKSPAWLQIKADVYGLPVETLSVTEAASLGAALLGRKAR
                     EKIEDFAGLINQLVQVREVYEPDKERNVLYKEKFEVYKRIYPALKNHIWKGER"
     gene            complement(37987..38952)
                     /locus_tag="PK016_00195"
     CDS             complement(37987..38952)
                     /locus_tag="PK016_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HXL01532.1"
                     /translation="MKSKGFATGVWVFGSCPDRFCTKGYKKEVSLEESLDKIASIEGL
                     RGLMMHYPAPLNENNAEYVKKEIEKRGLQLASCDVDLFSSPEFSWGGLMCEKEIRKKA
                     IEMSKKAMDIAEYLGADVMNLWPGQDGFDYPFQINYRAQWDLLVNALSEIASHNPRVK
                     LSLEYKLREPRTHSTIANAGLALFLSQTVGQNVGVTIDLGHSFNVKESPANVVALLDK
                     YGKLFALHLNDNFRDWDDDMAVGSVHFWETLEFIYYLYLSNYEGWLGLDIFPYREDPA
                     LACQFSIKMINYFLEIIQKIDRKKLETMQKEADALSALQYIKSLL"
     gene            complement(39487..40998)
                     /locus_tag="PK016_00200"
     CDS             complement(39487..40998)
                     /locus_tag="PK016_00200"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HXL01533.1"
                     /translation="MGIDVGTSGCKVVAFTEGGEIIASGYEEYPFLTPRSGWLELDPD
                     KIWEAVLNSLKKVAAQTGDTIDAIAVNSHGETLIPLGRDGQVLYRAIANFDTRSERYV
                     DFWKERLDPFQIFQITGMPLHGMYTVNKILWLRDEKPDIFEKVDKFCCVEDFVLHRLT
                     GEEPVIDYSLAARTMMLDVVHKKWSDEILHLASLDKDKLPRLSPSGEEVGHLRPSLAQ
                     ELGLSSIVMVATGGHDQPCGVLGCGVRKEGEAMYGMGTSDCVALNLGEKPRLTQEMME
                     NGFCCYPHVVKDSYLTLSYIASGGSVLRWFRDTFGGEEKEIAKKEGKDPYQVLIDTIP
                     SHPSSLFLLPHLAGSGTPYLDGYSRGTLLGLTFEASKGEIVRTILESLTYEMKINLDL
                     WEEFGIPLYNLRAVGGGARSTRWLQIRADILQKPLFPLFTEEAVALGSAMLGGKARGI
                     FSNLDEAIAAMVRFGKEVSPSPDKKEAYQLRYKIYQKIYGAVREINEEISKLL"
     gene            complement(40967..42157)
                     /locus_tag="PK016_00205"
     CDS             complement(40967..42157)
                     /locus_tag="PK016_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL01534.1"
                     /translation="MESKARLYLIIFLAFYLLAVSMIITGAVIPRWMDEFSVSAGRAG
                     RLFSFYYLPYTVVTFSSGLLSQRWGKKVVLLLGQAFLAGGFFSVSVSPSFSLIEGGLL
                     LLGIGGGLCEAPFTSLISQMFPGEEGYALNLSQISFGLGAASAPFLAGFLLERGFSWR
                     LFYLFPGIISFLIFALLSKEKFPQEKEIKEESRLFGPRGNGFIVVSFLAMFLYVGAEI
                     GSSSWIATYLVKGLGGSLSSGGIALAVFWSALTIGRFVFAFLSRSLSYPVILRFSSFL
                     SLIFLLFLNFTHEVHWAILALGGVGFGYSVIWPLIVAWVAQKMGERKEEAIGFVVAFG
                     GVGAMFFPWLGGVIGEKWGLSYIFWVVWVLVLFLWLVFHSSYFREGGEEIGFGGDRCR
                     DEWL"
     gene            complement(42147..43076)
                     /locus_tag="PK016_00210"
     CDS             complement(42147..43076)
                     /locus_tag="PK016_00210"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HXL01535.1"
                     /translation="MAKVKLGINNCFALGRYPEPEEWLRIVKKELGLQHVQFSFDLLD
                     PVIIDEEIVKEKCAFIRRLAEEQGVKIDTAVTGEAAHKFNLLLDPDPGMRRSYLRWYE
                     KMVRAASLLGAEGSGIYMGSLSNKDVNNPERKAYLTEVLIEEIAYLTFVAREAGQKYL
                     LWEPMSLPREMPSTIDETKELWERVNKKSHIPVLLCLDVGHGYYRSSDPKDLDYYSWL
                     RELAHLSPSIHMQQTDRKGSRHWTFTEENNAQGVIVPERVLEAIEASGAKEVVLVFEF
                     FFSAHAFSDESALDSMKKSVDYWREALAHFYGK"
     gene            complement(43081..45087)
                     /gene="pdhA"
                     /locus_tag="PK016_00215"
     CDS             complement(43081..45087)
                     /gene="pdhA"
                     /locus_tag="PK016_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HXL01536.1"
                     /translation="MGSKDKFLNMYRDMVRIRRFEETVSDLFSQDRIRGTTHLYIGEE
                     AVAVGACNAINPDDYITSTHRGHGHCIAKGATLDKMMAELFGKITGYCKGKGGSLHIA
                     DLNAGNLGANGIVGGGIPIAVGSGLTAKYKNTGKVTVCFFGDGAANTGAFHEALNLAG
                     VWKLPVVFVCENNLYAMSVPVKEAFPIQDIVERAQSYGMPGVIVDGMDVLAVKEAVEE
                     AAKRARRGDGPTLIECKTYRYLGHSKSDPRAYRTKEEENQWKERDAIKRYKKWLLDNG
                     VATEEEIKNIDAEVEKEVEEAVDFAEKSPYPPLEEITKDVYVEEDFAELERRKGTKVV
                     RGISEWKGEMRTITYREALNEALKEELKRDENVVLLGEDIALYGGAYGVTRGLWQEFG
                     GERVRNTPISEAAIVGCCVGSSITGLRPVGEIMYIDFVGLCMDQLVNQGAKIRYMFGG
                     KARVPMVIRTEGGCGRSSGAHHAQSLEAWFLHTPGLKVVMPATPFDAKGLLKAAIRED
                     NPVLFIEHKMIYNSQGLVPPEEYIIPLGVADVKREGKDATIIAYSRMLLFALQAAEAL
                     SQEGIEVEVIDPRTLVPLDMETIAQSVRKTNRVVIVHEGCKTGGAGAEIGMKIQEELF
                     DYLDAPIVRLGAADAPVPCSRYLEDNSIPRPETIVEAVKNLVRR"
     gene            complement(45090..46139)
                     /locus_tag="PK016_00220"
     CDS             complement(45090..46139)
                     /locus_tag="PK016_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent dehydrogenase"
                     /protein_id="HXL01537.1"
                     /translation="MKAAVFYGPGDIRIEERPVPSLKGREILLKVEACAICGTDVRIF
                     RHGHHNIHPPQITGHEIAGVIAALGEGVNGYKEGERVAVAPILHCGECFYCRRGLPNL
                     CLNFEALGYHHPGAFAEYMVVPERALRGGNINRIPDNLSFEEASLTEPLACALNGELL
                     SQVGVGDYVLVVGAGPVGCMHITLSRALGASKVIVSEVQEKRLLMSREFGADYYINPE
                     KENLKEKLLQVTEGVGPTVIIVAAPARKAQEEALDLAAARGRINFFGGLPGDNKIAQL
                     DSNLIHYKELLIQGTSGSTPLHNTWALEMMAAGRVEGKKLISKVVSLDELPQAIEEAE
                     SGNYLKIVVKPGIGG"
     gene            complement(46140..47324)
                     /locus_tag="PK016_00225"
     CDS             complement(46140..47324)
                     /locus_tag="PK016_00225"
                     /inference="COORDINATES: protein motif:HMM:NF019736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01538.1"
                     /translation="MKKGIQFGAGNIGRGFIGHLLWESGFEIIFVEAREDLARLLNER
                     KSYPLRLLRKDGKEEDLIIDNLRALSTQEKDKVAKEIKEGDVVFTAVGVKNLPFIAPL
                     LAQGLELRSQDGGFLNIFLCENLKDAPQLLRGEIEKYLSPAGKKFLEEKVGLVGTVVA
                     RMVPVMGERYGVEDPLFVVAESYHHLPFDAQAVKGEVPEITGLKAASNFPAEVDRKLF
                     IHNLGHATLAYLGYHKGYTYIHEAIKDEEIRKVLDGAWEEVSQALLRKYPDLNRREHQ
                     ELVDDLRERFANPLLLDTLGRVGREPLRKLAPDDRLIGGAHLCLSQEIFPHYIAIACG
                     AALSYDCPRDEEAVKLQKMIQEKGAEKVIEEICGAKAESKLGQAILSAYKNFSRERKE
                     WK"
     gene            complement(47321..48265)
                     /locus_tag="PK016_00230"
     CDS             complement(47321..48265)
                     /locus_tag="PK016_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HXL01539.1"
                     /translation="MSEITCIGILVADLVGRPIDRFPEKGRLMLVEDMELHAGGCAHN
                     TGVDLKKLGEEVKVVGKVGRDGLGEFMINSLVAHGINTGGISKDDENHTSATMVIVDG
                     QGERTFLHYPGANRGLREKDIADSFLSSSQVVHIAGSFLMPGFDGEETAKVLRRAKNL
                     GVITSLDTAWDDTGKWFSTIEPVLPYIDILLSNRDEAHHISGKTNLPDITQFFMNYGI
                     KVVAIKMGEEGSFIMTEEEKVLVPPFSVVAVDGTGAGDAFCAGFLVGYLRGWDLYETG
                     RFANACGAMCVRKMGATEGVGSLEETLAFIEEAEGKHR"
     gene            complement(48262..49212)
                     /locus_tag="PK016_00235"
     CDS             complement(48262..49212)
                     /locus_tag="PK016_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HXL01540.1"
                     /translation="MNNSDVEFVTRVAWLHFMEGMTQREIARHLNISQPKVARLIDRA
                     QKTGIVKISIESPLANCLLVEKKLRKLFPSLEDSVVVPVSPQRDLLETLGQAGAWYLE
                     RVLSDGDLVGIAWGRTLKQVALSIKSVSVKNLKFVTLVGGLTASASLNPYTIGERLAS
                     IFEGECYYLYAPAVVETEEIRDFYLRERINEKTLNLARQAKWSLLGIGAVDPHYSIYA
                     LTGFIDYHEWELLIQKGAVGDIVGQFYNQEGEIIDTPLHQRTVAIPLEDIRHMPHVIG
                     IAGGDNKVEAILGALRGGFINILITDEGTALKIIEKGEKR"
     gene            complement(49424..50155)
                     /locus_tag="PK016_00240"
     CDS             complement(49424..50155)
                     /locus_tag="PK016_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HXL01541.1"
                     /translation="MEDEEGRGILEGIFNFFLPQHCLVCGSYISCSQGFPLCPECFSR
                     IEYLNSPLCVKCGRPLPGPSTLLCHRCRTFPFHFDTARAVALYTSPLRDCIQAFKYQG
                     FTSFRYLFGELLCEYLEKNPFLKEVDCLLPVPLHPARLKERGFNQSLILAEEIKKCFS
                     IPIVSKEVRRIKPTLPQVGLKVKERRKNMRGAFEIKENNLFQNKRVLIVDDVLTSRST
                     VDSLSQTLKRGGAKEVLVLVLASGK"
BASE COUNT        15051 a        12325 c         8547 g        14339 t
ORIGIN      
        1 ctttaccagc caatggagaa tgcagagggg cagaaataaa agcttctaca tcagcgattt
       61 tctgtcctct ttttactttg tcacccttct taactaaagg cgataaggga gcaccggtat
      121 gttgaagaag aggcagaact acccgtgaag gaaggggaaa ctctttaata gtttttgtgg
      181 cagtaaattc tttaaaagta gggggatgta tccccccggg aaaagttttg agcttcataa
      241 atttactcct ccttcgacat tctaatattc ttccaaattc tcttccaaaa acctagtatg
      301 taaattgcca actctgaaat cttgattttg taaaattttc atatgaaaag gaatagtagt
      361 taaaataccc tccaccataa aagaatgcaa agcttcccgg gctctgcgga tggcaaaatc
      421 ccggtcgcag tcccatacca ccaatttagc taaaagggag tcataataag gaggaacttc
      481 ataaccagaa taacagtgag aatccactct gattcccggt cctcggggaa catcaaatcg
      541 ggaaattttg cccggagcag gagaaaaatt atgatttaca tcttccgcat ttatcctcat
      601 ttccagagcg tgccctcgta aaaaaatttc ttccggagaa aaagatagtt tctcacccgc
      661 tgcaagacca atttgttctc tcaccaaatc tattcctgaa atcatttctg taactggatg
      721 ctccacctgg atacgagcgt ttagttccat aaaataaaaa gaaccctcct cagttaccag
      781 gaattctact gtcccacaag tagtataatt cagtacttca gccactttta ttgctgtctc
      841 ccgtaatttc tctctcgttt cctcagttaa aaaaggagca ggagcttctt ctaaaatttt
      901 ctgctgcctc ctctgcagag aacattctct ttctcccaga tggctgacgt ttccttcctt
      961 atccctcaca atttgcactt ctacgtgcct cgctttagtc aaaagtttct ccatatacac
     1021 ctcctcgctg ccaaaagcta atttcccctc tcttttcgcg ctttctaaag cctcggccaa
     1081 ttgatgaggc tctcttacta ccctaattcc tcttcctccc cctccccgag aagctttaag
     1141 aaaaagaggg tagcctattt tttctgctat ctcttttgct tccttctcat ctttaatagg
     1201 ttctaaagag ccaggaacta ccggtatccc ggcttcttct agaattcttt tagcctgcaa
     1261 tttgtttttc atccgttcta ataagtccgc atccggtcca ataaaagtga tacctttctc
     1321 ttttaatttt ctggcaaatt cggcattttc agataaaaag ccataaccag gatgaagagc
     1381 actggtttcg gttagctcac aagcacgaat aattttttct tcgtttaaat aagtatcctg
     1441 aggtaaactt ccccctaaag ccactttttg gtctacaaat ttcaaatgca agagattgta
     1501 atccgggtca gaaaataccc ctacggtctt aattcccagc tcccgacaag cccgtaccac
     1561 ccgtaaagct atttctcctc gattagctat caatactttg ttaaacaaga ttctacctcc
     1621 taaataagga ccctatcttc cggataagtg attaaatctt ttcctcggga aggtcgtacc
     1681 attatcaaag tcataattcc cactcttcct acatacatag taacgataat tagaatttta
     1741 ctcaagtcta aaagattagg tgttatcccc cgagaaagac ccacagttcc aaaagccgaa
     1801 accacttcaa aacaaatatc cagaagagaa gcaggttgaa caaccaataa aatcataatt
     1861 actccaaaaa tcagaaggaa agctaaaact accacactca tggctcgaaa tactgcacca
     1921 ggtatcaccc gacgatgaaa gaattctact ctgtcttctc cttttattac acttactgct
     1981 gttcccaaaa gtaaagcgaa agtagtagtt ttcactcctc ctcccgttcc tcctggagaa
     2041 gcaccaataa acataagcaa aattataaga aaggtggaag cttcccgcaa atcagcaata
     2101 ggaatagtgt taaaaccagc tgttcgaggg gtaacagcct ggaaaagact ggccattact
     2161 tttcctcccc agggaagttc agccaatgtg gcaggattat tccactctaa aagtaaaatt
     2221 aaaacagttc ctaaagaaat caaaaataaa gtagttctta ccaccacttt agaatggaga
     2281 cttgatacgc tacgtttttc tactatttct cggagtacca caaaaccgag acctcccagt
     2341 attatgagcc ccataactgt aaaactaact accgggtcca gaacaaaact ctccagatta
     2401 ttactgaata ttgaaaaacc agcattacaa aaagcagata tggagtgaaa aatagcagga
     2461 aaaagagctt ttgccggtgt aaagtaacgc aaaaagttaa ggaagagaac cagagaacct
     2521 attccttcaa taaaaaaggt gtatttcaaa acggaaagga caaaacgtac cacccctcct
     2581 ggaaaatcca gactaaaaga atcacgaata gccaatcgag ttcgatattc aattctccgc
     2641 cggagagcta aggctaaaag agtgtttaag gtcatatacc ccagccctcc aatctggatg
     2701 agtgcaagaa tcaccagttg cccccagaag gaaaaaaaag tagcggtatc taccactatt
     2761 agaccagtaa cacaagtggc agaagtagca gtaaacaaag catctatgaa acgcagttta
     2821 ttccccggat ttaccataga aggtaaaagc aggagaagcg ctccactcaa aatgataata
     2881 acaaaaccta aaagtattaa agtggcggga gagaaattcg ccgctttctt ttgcactttt
     2941 tcccagagag agagtaattc tctactctcg gataataaca ccgtatcaac cttcttaatt
     3001 agagagaaaa agctccgtat tctttggctt cttcaaacat agtcattata ttttccggag
     3061 gaacatcagg ttgaatatcg tgcaccgaag ttagaacata tccttccggc cccaaatcat
     3121 ctattctctt tcttacttct tccctcactt cttctttagc cccctgagga agaacctgag
     3181 ttgtgtctat tgccccccaa aaagacagtt cccccccgaa cttttccttt aacactttag
     3241 tatccatgtt ctgagctgaa acctgaacgg gatttaagat atctactccc acttctatta
     3301 agtcaggaat gaaagaaaaa atactcccgc aggaatggta gagaaactga gcctgaggag
     3361 tcatcttttt taaattttta aaatactctt tgtgataagg tttaattatc tctcggtaag
     3421 ttttggggct cattatcaca ctttcagcag tagctaagtc atctcctaca aaaaccacgt
     3481 ctaaaaactc tcctacttcc tgaaggaaaa gagaatattt ctttagttgg taagtgtaca
     3541 tcccctctaa taaagtagta acaaaatcta catcggtcaa aagatctacc aaaaaatttt
     3601 caaaccctcg cagataccag caaggttcaa aaatgccggt atcaatcata tctcctacca
     3661 aaagatattc attgctctct ttaaggtttt ttgctttttc cctcaccccg gagaggtcca
     3721 tagttttagc tgggtctggc cattcataat cttgtagctc ttctatgctc ttccccgcta
     3781 agggatgctc taccatgtca tagtaaaggc catcttcggg catatgatac ttgataccaa
     3841 actcattaaa ataggttctc tcatctacca tatttttgaa aaacactggc cggggatgaa
     3901 tgccccgggt atctattccc aggatctgga gcacttcttc atcaataaaa acaatttgaa
     3961 agttctccat cataaattta gtttcttttt ctattcctaa atatctcttc aaattatcat
     4021 acgctacctt ggtcatagta gtacagttag tgctacccag atccagggga attctatctg
     4081 gaacctgatg atgcaatgct cttaaggctc tttcccttcc taacaatctt attctccttt
     4141 ttctgaaatg cataataatt taatagctct tatattttat cagaacaggc aaactttatt
     4201 tttataaagt tcatttagtt cattttcatt ttttaagctc atgccactca ttgcccagtg
     4261 ttatattata taatatctcc gtgaaaacaa ataccaacgc agctaatatt gctcgcctgg
     4321 gaattctggt ttccctgggc atagtgttac acatagtaga gagcctgctt ccttctttct
     4381 tcctaccggg agcaaagtta ggtctggcta acgtggtaac tctggtaatc ctgtggagag
     4441 gaaacgtggt agaagctata gaggtagcca ttttacgtat cgtactggga aatatcttaa
     4501 gcgggaactt aggaataggc ttttttctga gcttaggggg aggattagcc agcctggggg
     4561 gcatgtcttt ctttaaaaaa aggggggttt ctctctgggg agtgagtatt atgggtgctc
     4621 tttttcataa ctcaggacag ttattagtag cttatcttta tatgaaaagc gaaaacattt
     4681 tttattatct cccttttctg gttctttttt ctattcctac gggtttattg ataggttatt
     4741 taggacaatg tttacttcat tgggaggaaa aaatattctc tctctcttaa ttttcttccc
     4801 tttcacattg gcaaacttat tcttatcgaa taaaatataa agaatgcaat ataagataag
     4861 gagcgttaaa atgcaagaat tagaaaaaat ctggatgaac ggtgagttag tcccctggaa
     4921 agaagctaag attcatgttt taactcatgc tctgcactat ggaacagctg tttttgaagg
     4981 gattcgctgt tataaaacta acaccggccc tgcagtattt cgcttaaaag accatttgaa
     5041 aaggctttat gactcagccc atattgtacg catgaccatt ccttatcctc cagaggaatt
     5101 agaaaaaggg gtaatagaca tcattaaagc taatggagta gaagaatgtt atataagacc
     5161 cattgttttc cggggttatg gagagatggg agtagacccc tcatcctgcg gcgttgattg
     5221 tgtgatagca gtatggtgct ggggagccta tatgggagaa aagggaatta aagaaggagt
     5281 ccgagccaaa atatcttcct acaccagaac ctacattaac tctggctctc cccgagctaa
     5341 aagcagtgcc aattatctta actcaagtct agccaaaata gaagcaaaag atttaggtta
     5401 tgatgaagcg attttattag atattaatgg cttcatttcc gagggaagcg gagaaaatgt
     5461 attttatgta aaagatggag taatctttac cccccatccc tactccattc ttttaggaat
     5521 tacccgggac acagtaataa aaattgcccg caaactgggt atggaagtga aagaaaccct
     5581 ttgcacccgg gatgaccttt accttgccga cgaggctttc tttactggaa cagcagcaga
     5641 aattacccct attgccgaag tggatggccg aaaaatcggt acaggaaaac gaggagaaat
     5701 aacccatctt ctgcaagaag ttttcttccg agtagtgaga ggggaagaac cagattatga
     5761 aagctggtta actaaattaa ggtgaggtga agaaattgac taccgacccc cgagaatttt
     5821 tctcggaata ttgagaaggt ttgcgagcca taaaagaaga agctgtctct acaatagaag
     5881 ggtttaacca tttttatgga aaagtaatga atgaagggtc attaagtctt aaagttaaag
     5941 aactgatagc cttagcagta ggtgtagctg tacattgtga aaattgtata atgattcaca
     6001 cccggggggc aaaaaaaagt ggagccaccc gagaagagat tttagaagca gctagtgtcg
     6061 gagtagtgat gggaggaggg ccggctttta cttttctccc tctggttcat agaactctgg
     6121 atagtttgga aaaagaataa aagattttac tggtagatta tgccttacat agctttttta
     6181 accgactggg gatataaaag ttattacgta ggggtaacca aagcggtaat taagaaaata
     6241 aatccccagg tagaaatcat tgacttaact cacgaggtgg aagcttttaa cgttcgagag
     6301 gcaatgtata tactctaccg ggcgttcagg gacttccccc cggaaagtgt atttttagct
     6361 gtagtcgatt atggagtagg aacagaaagg aaggctttag ctttacaggt ggaaaaaggc
     6421 tacacttttg ttggtcctga taacggcctt ttcactttaa tagttgaaaa cgaaaaagtg
     6481 gtaaaaatag tagagttaaa ccgcactcag tatttttacc atcctctccc ctcttctact
     6541 ttccagggaa gagacatatt tgccccctgt gccgcccatt tatcgagagg tataccaatt
     6601 gaagagttag gctcttctgt agacttcaaa gatttaattc ttcttcctta tgaaaaaccc
     6661 gaaattaaaa atcactgttt gcatggtgag atagcctatg ctgaccgctt tggcaattta
     6721 caaaccaacc ttcctggata ttattttcaa gaaatagaaa taaaagaggg aggaaaatta
     6781 actatcgagg tgggagagaa aaaatttata gctacttatc accccgttta tgcttttgcc
     6841 tctaaaggag agcttctaat tcatcctgat agctcaggat atttagaaat cgctgcttac
     6901 caggaaagcg ctcaagcaat tataaaagct cggggaggag aaaaaattct cctccacaaa
     6961 attgtttctt aaaaggaact taaatcgtca aaaacgggaa gaagagaaag ataagctttt
     7021 ttaaagatgg ggtataaacg attgtaaact tccaccattt ccgctcgagg tgaaaaatgg
     7081 tcttctatct ttaccagttc ttttgctact tctaaccctt taaatatccc cgcccccact
     7141 cctccagcaa tagccgctcc taaagaagta gcttcctcta gataccgggg acgtaaaatt
     7201 tccaccccta aaacctgagc taaaatatga gaccagaaat ccccttttgc tcctccacca
     7261 atgagacgca aagtatcagg acaaaggcct tgttctcgaa aagcatcgag tattatccgg
     7321 agattaaggc tgactccctc taaaactgag cggatgagat gagacttatt atgagaagga
     7381 gttaagccta tgaatacccc tcgagcatga ggattccacc agggagctct ttctcctaaa
     7441 agataaggaa gaaaaagtaa acccccacac ccgggagaga ccttactggc ctcaaggtcc
     7501 attaattggt aagcactcac tcctaactct tgagccagaa ctttttctct ttcacaaacc
     7561 gcatcccgac accactgata tgacccaccc gccgattgca tagtgcctgt aggcataaaa
     7621 agaccttttt ctaagtgatg aaaggtaaaa gtccttttct ccgggtcata aaaaggttga
     7681 gaagaggcaa aagcaatcca ggaagaagaa ccaagataaa ggtaagcttc ccctttttcc
     7741 actattcctg ctccacaagc agcacaaggc ccatcccctc cccctataac taccggagtc
     7801 ccaggtaaaa gtccacactc ttctcccgct tccttagtaa gctctcccac caccgtcgta
     7861 gagggataaa gaggaggaag tttatcctct tctattccca ctttctctaa tatctctccc
     7921 gaccaggttt cccgagccag atcaaaaaga ttagttcctg acgcatcaga aaagtcggtt
     7981 gcccattggc cagttaacct gaaaactaca aaatctttag ctaaaagaaa cttataagct
     8041 ttattataaa cttccggtaa atggttccga aaccagagaa ttttagccag agaatagtta
     8101 gcgctgagac gatggccggt caattggtaa attcggtctt ctcctatcct ttcttttatc
     8161 cattctactt cttctatccc tcttctatct gcccagataa gaatatgata aagaggttgg
     8221 cctttttcat ctactggtag tgctcccatc atttgtccag agaaagaaat aacctcgaca
     8281 tcttcctttt tcacttttcc tttttccagc aactgacgag tagaataaac cactgcctgc
     8341 cagtaatctt ctgcatcctg ttctaccgaa agagcgtcag ggaaataagt atcatatcca
     8401 aaaaattcac tggctatttt ctccccttct tcattaaaaa gagtagcttt gttaccagtg
     8461 gttcccaaat catgagctat aattagcttc cccattttct ccttcctttt tctttttcac
     8521 caaaagacgt aaggtaagaa ttctttttcc tttcattctt tctactttca gttgataact
     8581 atttacctcc accatttctc cttcttcagg aactttccct aaaagttcca taactaatcc
     8641 ccccactgac tcaaactctt cgctttcagg aagtaataag cctaaaagct cattgagact
     8701 ttcaatggga agagaagcat ctaccagata ttcattatcc ccgatctttc gataaggagc
     8761 agtttcccgg tcatgctcat ctcttatttc tcctactaat tcttctaaaa ggtcttcgat
     8821 ggtaaccaac cctgctgtac ctccatactc atccagaacg atagccatat taattctctt
     8881 ctgttgtaac tcttttagta attccatagc ttttttagta agaggaacaa aatgaatggg
     8941 gcgcattatt tctcttaccg gcttatccat atctccggta tttaaatggg gtaaaatatc
     9001 cttaatgtgc acaaacccca gaatattatc taaatcaccc cggtagaccg gaagacggga
     9061 aaaatgactt tgcagggaaa gctctaaagc atcttttata gtagcattat cctctataca
     9121 taccatatca gtacgaggag tcatcacttc ataagctaaa gtatctccaa aatcaaagac
     9181 ttcattaatt aatctcctct catcgagtgg caaacttctt tgttcagcaa taaataattt
     9241 taattcttca tcagtaactg aatacactga ctcctgggga actcccagaa tgcgggatat
     9301 aaaatcagta gaaacagaaa gaagagtaac cagaggttta aacgctcgag ataaaaaaac
     9361 tatgggccga gctattcgaa gagcaaattt ctccgggtta gccaaagcta ctctcttggg
     9421 aaccaattct cccaaaacca gactcaagta agaaattata aaagtaatca gaataactgc
     9481 cagctcttct ccaagagttc ccagtatggg aagggaagta ctttccaatc tcctacctaa
     9541 ttctcgagaa agagaaactg ctccagtagc gctggcaaaa aaaccagcca gggttataca
     9601 aatctgaatg gtagctaaaa actcattggg cttctccagg aattgttgaa tagcctctaa
     9661 ttcctgggaa tcttcctctt tcatcagcct caattttacc cgacgagtag aaatcaaagc
     9721 aatttccgaa gcagcaaaaa aagcattgat aaaaatcaaa agcaaaatga gaagaatagt
     9781 agctaaatat cccataatgg ttaacgcaac ctcctggaag gataaaaaaa tagaatatga
     9841 aatttattca ggcagttatt agaaagatag aacggaggca tagcttgaac cacacagaaa
     9901 aaaaaccaag cagcaagcgc tctttggcgc atgttactcg gagaatctac atcttcagac
     9961 attaattctc acactctcct tttacattga tatcttatca tatttaagaa ataaaaacac
    10021 cttttaactc attctcctcc cagaaagcgc agagctaaaa gggagtagat gcgagaagct
    10081 gccactatct gggtgacaga aataaactcg ttggcagtat gagcttcttc gattctaccc
    10141 ggtccaaata tcaaagtagg tatcccggcc tctactaaaa aagaagcttc acaggaagca
    10201 gggaaaggag caaaagtggg aatctctccg ggaacatcac tgatagagcg agagagaatt
    10261 ctaactcctt ctaagtctcg actgattttt actggaggaa gatagggaat agattggtca
    10321 atctcataat taaactttaa ctctttttct tgttgaacat catccagaat attttgcaaa
    10381 gtttcttcca tcttttctaa aggaaaatca ggaacggtac gcacgtccac ttcaaaactg
    10441 caaatatcag gaactacatt aggaaatgtt cccccttgaa ttttacccac atgtaaagct
    10501 ggattcccca gatctgggtc agtaaatttt ttcaattctt gattctttgc ttctatagat
    10561 tgtattatcc gggaagcaag gtaaatagcg tttattcccc gctcggctat tcccatatga
    10621 catgatttgc cctgcacctt taactgataa gctgccactc ctcgatgacc taaactgata
    10681 ttaagaccgg taggttcacc taccagggca aaatctatca tttctcccct ttctttataa
    10741 gattttatcc agtgttttac tcctaaatta cttccttctt catccactac cagaactaat
    10801 ttcagcttgc cataaaggga aggagcaaac aaggaaacca tataagcagt atacatcata
    10861 gaagctaaag ctcccttcat atcacaggct ccccgtccat acagtcgatt ttctataatt
    10921 tctcctccca gaggaggata agtccagcca ctacctaagg gaacagtatc aagatgacca
    10981 ttaaacagaa ggttaggacc ttctcctaaa attccctcta cttcggcaat cagattagct
    11041 ctcccctctt ctacttcctg ccattccact ttaatctggc ggtcgataaa gtaattggca
    11101 atgaaacgag ctagttctac ctcgcctcct tctcccgaga cactgggaat acgcaccaga
    11161 ttttgagcca gattgatagg ttcaaaaagg tcgaattcat tatccaacaa agctttccaa
    11221 tcaggcacaa ctttcctcct atgaaaggta acgactacct cttttttgca aggcaatttt
    11281 ttgctggcac agaggacact cttccgggga ataagtttgt gcttctatct ccagaagagg
    11341 ataaaaaggg taagagaaac tagctttctt tccactccta tccaccagaa ctcctatccc
    11401 tacaatctgg cttccggttt cctccactcg ttttatcact tcttctaccg acgctccagt
    11461 agttaccaca tcttccacaa ttaaaacccg gtctcctttt ttcaactgaa aatcacgacg
    11521 gaaaaccatc tttccttctt ctcgctcggt aaaagccatc tttactccta actggcgagc
    11581 tacctcataa gctaaaatta tcccccccac tgctggtcct actactacat ctattttctc
    11641 tcctgaaaag tggcgaatta tttccccgca cagaatctcc acatagcggg gaaattgtaa
    11701 aatcttaaac ttttctatat aaaaaggact gtgcaaacca gaagtgagga gaaaatgccc
    11761 ctccaaaaaa gccccagcct ggcgaaaaat ttctaatatt tcttcctttt ccattgtatc
    11821 acttcaccct cccaattaag tcttctaaat tctctatttt tctatcttct aaatactgtt
    11881 tgattccctc taaaatagac aaagctgaag aaggctccag aagattagcc actcccagag
    11941 ccactgcttt tgcccccacc aaaaggaact ctaaggcatc gtaagcagta acaattcctc
    12001 ccattccaat aactggaagg gaggtagaat ttactacctc ataaatcatc ttcaaagcca
    12061 gaggcttaat agccggacca gaaaaaccag cgaatttccg tttaaagaaa aaattcatgt
    12121 tttctacatc tataaaaaga gcgggaaaag agttagtaag gctaaggata tctgcaccct
    12181 cactttctaa aatatccaga gtctttcccc actcctttac ctcaggacct aacttcacaa
    12241 tcagaggttt ttgagttgca tttctcactc tacccactat agattttagc atctctaagt
    12301 cagaacaaaa aaccgctcct cctctctctg tattaggaca ggaaacatta agctccagag
    12361 catctactct ttcaaactta tctaactctt gtgctacctg aacaaactca tctgccgttt
    12421 ctccccagat gttaaccaaa atacgagtat caaaagaaga aagaaaagga agatcttccc
    12481 ggataaagcg atgaacacct ttattttcca aaccaatcga gttaagaatc ccagcgtata
    12541 cctctcgtaa tcgaggagga ggattaccag gaaaagagta ataacttaaa ccttttactg
    12601 ttattgctcc caccaggttt aaatccagat agggagcaat ttctcttcca taaccacaag
    12661 taccggaagc caaaattact ggatttttta aattcaatcc ccctaagttt actgccagat
    12721 tcaccatttc agttcctgag ccaaaaatac cggaccatct ttacagacat gaaaataacc
    12781 ttcaccatct cccttttcta ccgcacaact catacacacc ccaaaaccac aacccataaa
    12841 agtctccagg gaaacttcta aggggatgtt ttgtattcct tcttcttgat aaagatttct
    12901 taacattccc tctggaccac aggcatacac tctggtccct gtagagagag ctttatcttc
    12961 aagccaggta cgaaaaagag aaagtactgt cccgcaaaaa ccatcctcta ttggctcctc
    13021 acaactccag aaacaagcac cagaaaaagc ttgcaaaaaa ttaaccataa aaagctcttt
    13081 tttatttttt acccccagaa aaaaaataaa agggattttg ttttccttaa aatgagaagc
    13141 tagaaagtaa agaggagtta ttcctctccc tccagctact attaaagcct gagagggaga
    13201 aagagagaat cccttaccta gaggccccaa tacttctaac ctgcttccca catttaagga
    13261 agagagaatt ttcgttcccc tgcctaccac ttcgaagaag agatagaaac tatccccctc
    13321 tactttcata atcgccagag gacggcgcaa taaaggatca aacccttctt gtactctcac
    13381 catgacaaat tgccctggtt gagcgcagta ggctatctcc ttctctttta atttcagctg
    13441 gatgaatcgc tctctccagc actttttttc ttctattata gcttttttca ttttaaaatc
    13501 tctcttaatc tctcctggta gaatatagct ctctcttttg ctttttcttc aaacccccaa
    13561 cctttgtcat tcttttcctt ctgatagtaa aagattatgt ctcgagagat ggtaataagc
    13621 gctccctctc catctttccg aaaagcaaaa cgaagagaag aaaaatctcc tccctgagct
    13681 cctattccag gaacaagaaa taaaagggaa gggaactctt ctcgcaaaaa cctcatatct
    13741 tcaggataag tagctcctat cactattcct aaagagctaa atccactctc tcccattact
    13801 tcttctcccc acttttctac caaccgagcc actcgaacga aaactttttc ttctcccaca
    13861 tcctgaataa aaggagcaga aggattagta gtacgggcta atacaaatac ccccttatcc
    13921 tcttttttag cttcttcaaa aaaaggctgt aagctatctt cacccaaata ggggctaaca
    13981 gttatagcat caacattcca aaaagagggt aaagttaatc ttttccctat ctctagctga
    14041 gaaagataac cttgagcata aaaagtagca gtgctggcaa catcgtttct tttaccatcc
    14101 agaattacta taaaatcctt tctcctggct tcatcaatgg tctctttcaa agctatcatc
    14161 cccggaactc ccagagcctc ataaaaagcc acctggattt ttgcaaatcc agctaccgag
    14221 gtaagagctt gcaaaataag agaattaaat tctaaaacgc aatgagaaag cgcttcccac
    14281 gtctttccct ctttctctac ccatttttcc cagaggtagg gaggtagaaa ctgaaagtga
    14341 gggtccagac ctactactaa gggacctttt tcctgggttt tctttatcgc ctggtcagca
    14401 aaattcactt tctacaacac tcccttctac cattactatt ttaccaccta ctatagtgta
    14461 aagcggccat cccgataaag accagttttc aaaaggacaa tttttacctc gcgattcaaa
    14521 gttagaaact tctactactt ttttagtttc cggatctaca atagtaatgt cagcatcttt
    14581 tccttcttcc agaatccctt tgttgacgag tcctaaaaag agtgctggat tataactaag
    14641 tttttcccat aattccaccc aatttattac cctttcctct attaaagctt tggcgtaaag
    14701 aggaacggtt atttccaaat tggatatccc gaaaggtgct tcgctaaaac tgcgatttct
    14761 gtcttcagag gggtggggcg catggtcaga agcaagtata gagattaatc cttcttttat
    14821 tccttccttc agtacgtttt ggtcttctaa agagcgaagg ggaggcttta ctttggcttc
    14881 gtttcctaac cctaaaacat catcctcagt taaaagtaga tgatgaggag tgacatcggc
    14941 actaatcggc atttttttct gaaaccagcg cagaagttcc acttctaaag aagtagaaag
    15001 gtgggtaaaa tgaacccgag caccagttcg ataagctaga accaggtctc gggccatcaa
    15061 agattcttct gccactcgag gtatagctct ttttcctaaa acaatagagg ttttcccttc
    15121 actaatttcc ccatcccgag atagggtttc atcttcttca tggagaataa aaggaagatg
    15181 aaaatattga gcatactgga aagctaaata gagaaggtga gcatcctgta cccccttgcc
    15241 atcatcactt aaagctacca ctcctgcttc cttcagcaaa ccatattctg ccatttctct
    15301 tccttctaaa cctacagtta tcgctccagc aggaaaaatg ttaaccagtc ccacttcttt
    15361 ggctcgaagg agaagataac gaaccagaac aggattatca atggggggat gagtattggg
    15421 catagagact actgaggtaa acccaccccg ggctgcagct cgacttccag taagaaggtt
    15481 ttcctccgat tctcttcccg gttctcgcaa atgaacatgg agattgataa agcccggacc
    15541 cactaaaaga ttagaagcat tgatgagaaa agcctgagca tcttctatgc cttttcccat
    15601 cctggttatt aacccatttt ccaataatat atcagcatcc tggaaaactc ctttttgagg
    15661 aaaaaccaga gtcccgcctt ttatgagaat ttttttcatt gttgctctcc ttgaagcaag
    15721 gtttctagaa ctgccatcct tatagctact ccacaggtta cctgttcttc tatcagagaa
    15781 aaatcttctt ctgcaagact ataactaatt tctactcccc gattcaccgg tccgggatgc
    15841 ataataaagg gcctgccatt ttcccggtcc agccattttt cctttaaacc aaaaaaatga
    15901 gcatattccc gaatactggg aaaataaccc gcttcctgtc tttctttttg aatgcgcaaa
    15961 agatatatta catcaactcc ctccactgct tcttctacgg gaaaaactac tttagcaccc
    16021 agattttcca tcgttggagg aagaagagta ggaggaccag agaggaaaac ttctgctcct
    16081 aattttttca aacccaaagc aagagagcga gccacccgac tatgtaaaac atctcccact
    16141 atagctactt ttaatccttg aaagtagcca aattttcttt gcaaagtcaa aaggtcaaga
    16201 agagcttgag taggatgttc tcgcatccca tcaccagcgt ttatcacgtg aaggggtaaa
    16261 agttgagcca gatactccgc tattccactc tggcgatgac gtaccactaa aacatcaaac
    16321 ttcattcgag aaagagtttt agctgtatcc tccaaacttt cccccttctg gaaactactt
    16381 atttcagtag taaagtttac cacctccatc cccgagagcc gacccgctac ctcgaaagaa
    16441 actcgagttc gagtgctggg ctctaagaaa aaattaacca gacaataacc ttctccatac
    16501 ttcattttct tgggagagca gttcaaaaat tctcgaaaat agttccccct ctccagaaga
    16561 tactcgatct cttttccagt caggagatta attcccagaa ggtctttata cttccatttc
    16621 acttttccaa ccctccctgt tcaaaattaa ctaatgagac aaatcaatta tctttatcat
    16681 ttcttgcttc aaaatatggt gatattctat atctcattaa ctctgataag cttcttggta
    16741 tctctatcaa cgatacttca ctaacctcga gttattaaca attcaatcat attttctggt
    16801 aagaatggtg tctttttaat atttctccca catccattag aaaaaaatgg ccatactttt
    16861 tgctaaaagc atacaaaaac ccctcgtact taacgagggg tttttgtatt tttgaatttt
    16921 tcttttttca tttctaaaac cctgaattag gatactcata aattattcct ctgtcaatca
    16981 tcaagaaacc tggattctct acccacggtt catgtgcatc acaagctgtt ccgtttaatt
    17041 acaaccctga atcatcaaaa agagctgtaa aagatcaatt tgtccgatga agaaagctgc
    17101 ctacttttat ataaaaaagg gtttatctca tcataaacca atcatttcct ctattttttt
    17161 aatgtttaag tgccgtttta aaattttact ccaccaatct aattcctgat ctacttcccc
    17221 gagagaaaaa tctccccccg agcaggaaaa gcagtcttta ccacctggtt tctctaaaag
    17281 gctatcttct tctactaagg atagagaaac atagggtaaa actcctacta ccggaacttt
    17341 ggtgagttct tctatttccc gcaaaccagg ctttaaaatc tctgcatctc ctcgaaattt
    17401 attgataata aaagcccgta ttaactctct ttcttctttc gccagagccc aggtcccgta
    17461 taaagaggca aaaacccctc ccctttctat atctcccact accataactg gagactgaag
    17521 ataaagagcc actttcatat tagccatatc ttttccccgt agatttatct ctgccggact
    17581 tcctgctcct tctaaaataa ggaggtcata ctcctctccc agagagtcaa ggctttcttt
    17641 aatcacttcc cagaaaaact ccttatcctg ataataagaa gaaactttcc ccatagccca
    17701 ggtttttccc cggattatta tttcgctttt cccatttcct tgaggtttta aaaaaatggg
    17761 attcatccgg ctgtcaggtt tttgaaaagc agcttgagct tgaagaattt gagctagggc
    17821 tatttcttct cccccctcag taattccaga attacaggac atattctgag ctttgaaagg
    17881 agccaccttt attccctgcc gggcaaaaag tctcaccaat cctgccacta tcacactttt
    17941 acctactcct gatcccgttc cctgaaccat taaagacctg tttttcatat ttcctcaaag
    18001 ttgctaaaaa cccggggctc tttcactccc cataaaatcc atagcaagcc tatcacattc
    18061 aaaatcagag ctaaccaaaa aacccagtag taaccaaacc aatcggcaat ttttcctcct
    18121 ataagaggag aaaaaaaagc gggaaaatta cttaaaaaac tgctccatcc cagatatagc
    18181 cctcgccttt ctcgaggagc aaaatcgaga acaatggctg ttcctcctac cgatatagca
    18241 ctataattaa agcctaaaag ggcaaaagct aagaaaaacc accgatagtc gggagaaaaa
    18301 agagccagca aattactcaa aaccatagat gtagccccca aaagaactac taatttatgc
    18361 ccctttttat ctcctaaagg accccagata aagttactca aagcctgaga gacaagtaaa
    18421 acagcattat agcgaggcac caaagcatca gggatttgaa atttctgtaa agtataaaca
    18481 gtataaaaag ctcctcctac tacccctaaa gctaaaaata cccgagctaa cataaaactc
    18541 aaaaagttgg catcttcttt cattacccgg aaaaaagagg gaagaaattt ttctacagat
    18601 gaagaagttt ctggctcttt ctcctctcga gtaagagcca gaaaaaaata agaaactacc
    18661 attgataaag aagccagcag gaaacaataa ccaaaattgt gagaaaaaga gtaagttttg
    18721 aggaactttt ctgctaaagt agaaccccac atccccatca gagctcccac gccgttacca
    18781 caggcaaaat acagccccca gcgactgtta tgaatgactt tcccaatcat ttccgtccag
    18841 atagggccca gaaatcccat agcaaaagaa ttaattccta acaaaccaaa cataaggtaa
    18901 agtgataaag aaggataata tggaacgagg taaaaagcta caagcgccat ccccaagaaa
    18961 ggaagacgtt caaaaagggt aaatttaaga ataaaaggaa gtttcctttc taacttgcct
    19021 gaaactcttg ccccccagat ggcaggaaat gaccacccca gataagcaat ggcaggaatc
    19081 aatccaatct ctaaattgct agcccctaaa cgctgagcaa aaagggggag cagtgtaacc
    19141 gtagagccaa aagacatccc caagctaaaa aaagcataat ctaaagcatc tactgcaaag
    19201 ttaaaaaaat aatctcgtcg agaaagcatt actctcttct tcctcctcgc cacttaatgt
    19261 aagaacgcgg gtctaattcc tgcaagaatc gagaaggttt cctgaccata gaacgagtac
    19321 ggtttctttc cgctaaaata ggataacaca aaaacaaatg ttctcgagct ctggtacaag
    19381 ccacataaaa aagtcttctt tcttcttcaa tatcctcttc ttttcccaga ctccgaggag
    19441 aagggaatcc tccttcacat agccagacca caaatacaca atcccactct aagcctttag
    19501 cctgatgaat ggtggaaaga atcactcttt catcctctat tccccctctt accacattct
    19561 ctgcttctat ctcccctaaa agagctaact cgctcaagaa agtttctaaa gatttataac
    19621 gcaaagcaaa gttagccaac tcttccaagt cttcttctct ctcctcataa ttaggataat
    19681 gagtaattaa atagtctcgg tatcctcctt ctaatacttc ccgaattaaa gtagaaggag
    19741 aggaatccaa atcttccaga cgaaagagta aatccctaag agaagcaagg ctaacccggg
    19801 cgttgaccgg aagaagagag aaaatttcct ccctctccaa agtagtaaga gggtcgggag
    19861 aggaagaaag agcttcccac actttttctg ccgatgctcg accaattccg ggataaagtt
    19921 tgagcactct cttccaggcc atttcatctc ggggattagc tattactttg aggtaagcca
    19981 ccacatcttt gatatgagcc tgttcaaaga aacgcagccc agagcgaatt tcaaagggga
    20041 tgccccggcg agtgagttcc atctgaattt ccatgctgtg ataatgagct cgatacaaaa
    20101 cggcaatttc ccggaggtcg tgaccttcgt tgtgtaattc taaaatctgg gaagccacaa
    20161 attctgcctg ctccataacg tcacgcaagg gcactactac aggcttttct cctccttttc
    20221 tcaccggatg taaaacttta ggaaactgat gaaaattaaa agaaatcacc tgattagcta
    20281 aatccagaat aggagcagta cttcggtagt tggtttccag tttaaatatc tgacaaccag
    20341 gataccgctc gggaaaacgc atgatgttaa caaaattagc tcctcgaaag gaataaatac
    20401 tctgagcatc atcacctacc actaaaagat tgcgatgttc ccgagctaaa aggtctacaa
    20461 tttcagcttg caaaaggttg gtgtcctgat actcatctac caaaatatga tgaaaaacgt
    20521 ttccatattt acgacgggta ttttcatctt cctgcaaaag agaccaggtg tagtaaagca
    20581 aatcatcata atccataaga gagagctggc gttttctatc ctggtagaga gcaaaaaggg
    20641 agcttatctg acttacccac tccagaaatt gaggataaaa cttttctaaa atatcttcca
    20701 cagagataaa agtattacgg ctcaaagaga tgatattagc aatcacatcc cctttaggaa
    20761 aacgacgaga acgacggtct attcccatct cctctatagt ggcttccaca aggtctttct
    20821 ggtcttcccg gtctagaata gtgtagttgt tatcataacc tatttttttc gcttcccggc
    20881 ggagaatgag gttaccaata tgatggaaag tccctcccca catcccctcc agacttattc
    20941 ctaggagaca ttccactcga tgcaacattt ctcgagcagc tttattggta aaggtagcca
    21001 aaagaagtga ggagggatct actccctcct ctaccagtct tgccactcga taggtaatag
    21061 ttcgagtttt tccactacct gcaccagcaa taaccaaaat cggcccataa ctagcgaaga
    21121 ctactttttt ctgctcctca ttcaattctt cttcataatc aatgcgcaaa attcctttca
    21181 aaagtcaaac ctcctattta tttccttttt ctatcagctc tttaagctcc ctgtctccaa
    21241 aaagataaac ccgattacaa aaaggacagc tcacttctgc tcttccctct tgagctaaca
    21301 aatcttctaa ctcttccttc cccagtaaaa ttaaggtcct acgagccctt tcccgggaac
    21361 aatagcaatg ataatgcagt ttcttttctc ccactatttt ataagaaaga gaagccaaaa
    21421 ggttttgaat tatttcttcc ggacttttac cctcaaaaag aagagagctc accggaggaa
    21481 gaacagaaat attatcttcg attttcttta ccactgcaga tggagtacct gctggagtat
    21541 ggatgataaa gccacctgct gataatacct ctcctttctc tcccacataa accccggcac
    21601 tgcaagctga aggaagttgt tcagaaagag caaagtaata agctaagtcg taagcaatac
    21661 ctcctcgagg catttctata taaccagcat aaggttctcc caatcccagg tctttcacca
    21721 ctgaaagttg agaacctttc cccactgctt cttccacatt aagtttttta tcttcccggg
    21781 aaggaataaa aaccaaaggt tgagaaacat agcctctcac ctctccctgg gagttagcct
    21841 gcaccaatat gctctctaaa ggtcccaagc aacgaatgta taaagttact ttctgacctt
    21901 ccttgagagc agcacccatt atccctgcca aggtcagtgc tcttcctaaa gcagcggtag
    21961 ctacgggact ggtattatgt aaaaaccttg cttcttccac taaagaggta gttgaagcca
    22021 cataaacgac cactgtggta tcttctacta ttgtgcatac cactttatct tcatagtttc
    22081 ccactcaatc actctcctta acagccatca tccatagagc aaaaactaaa aataaaaata
    22141 atacccaggg gctgctatcc caaaaggtat cacttcctcc ccatttagac aatacctgaa
    22201 gataggcaag agctaaaaga gaacttccca ctaaggagct aaataagttt aaaacttttt
    22261 tattcccgaa aagaaaccca gaaattagag aaaaaagaac cacttcccta aaagagagat
    22321 gctgcaaaaa acgcattaat tccaccgaag aagaaagttc gattaaaaga taatagaggt
    22381 tggcagctaa ccagaagcca gaaaaaaatc ttccccattt ctctcctttt ttgctcgctg
    22441 ctactcctgc taagaaaaga gccaaaaaga gaaaaacgaa gaaattcact tttccgtccg
    22501 aaaaagaaag agtttctaaa ttactttttt ctattcctcg agtaatacct cctccccaca
    22561 acaccactac aaaagttaaa agggggaaaa gaatcttttt cagacgaaaa aaataaaaac
    22621 cagaaataat ccctccccct aacaaaagaa aaatcaacca cccttctcct attttctcta
    22681 tttccaatag ccagtaatca agaaacaaat ttattcctcc ccggagagaa tcagctgagc
    22741 agctttagct actacttcag aaagccggtc ttctatctct tctattttct cctcttctac
    22801 ttcttctttt tcatttctat taaccagaac tccacagatt gccccggagc ggagtcctag
    22861 aacccgagcc acggtaaaaa gagtggctac ttccatctcg taattcaaaa cccccagcct
    22921 cttccattct tctctactac cccgaagaga agaaataata taaccggtaa aagtatcata
    22981 cctttcctga ccgggataaa aagtagctga agagcaggta attcccaagt gatagcgata
    23041 cttcaaaagc tcacaggctt ttttgagagc tcctaccatt tcccagtgag cacaggcagg
    23101 ataaggaaga ggagcatagt gatcggaggc gccgtccatt ctcactgctc cttcgctgat
    23161 caccaaatcc cccactgcaa ttccttcctg aatggctccg caggttccta cccgtaaaaa
    23221 aagatttact cccaattgag ctaactcttc cactgctata ctcaaagaag accccccgat
    23281 gccagtagaa actactaaaa ttttaccact ctcattttct gcttttaccg tcttaaattc
    23341 ccgatgaaaa gccaattctt caaaactacc aaaggcagaa gcgagcttac agcatcgctc
    23401 cggtgccccg ggtagcagag ctaattttat tccccttact tcctcttctt ttaaagctaa
    23461 atgataagca taataaccca tatctcttca cctcttcctt attttcggct aaaatagtaa
    23521 aaaggggaag agataaatgc caaaaaaaat ttgttatctt tactgggaaa aagaaaaaat
    23581 acctgtccca cggggtaaaa ctttgcagga ggtagttaaa aaaatatttc cctcctggga
    23641 aaggattatg gctgcccaat tagaaggaga aatagtagac cttaacaccg aagtagaaag
    23701 agatagcaaa gtaaagtttt ttgacttctc tcatcctgag ggcagaagaa cctacctgcg
    23761 aagccttctt tttgtcctga gctacagtgt agaaaaaatt tttcctggcc atcgactaaa
    23821 agttctttac tccttagggg aggggttaat ctgtgaaata gaaggagtaa atatctcttc
    23881 cgagcaatta gagcagattg aagaagagat aaaaaatacc atagcagaag accttccttt
    23941 tacccgagaa gtggtagact gggagaaagc cactgctatc taccaaaagc agggaagaaa
    24001 agacctggtg aaactcttcc gttactggag aactccagca gtaactgttt atcgattgaa
    24061 aaacttctac gaccactatt atggacctct tgttccttcc accgggtatc taaaaaattt
    24121 tggctttgtt ctggttcctc cacaattcgt aattcagttg cccaccggag aagacttaca
    24181 gcgtctccct acctatactt ttcgccctaa gcttttcagt accttccagg aagcggtacg
    24241 ctgggcagag attttagcta ttgaaaatgt gggagagtta aatgaagcca ttgctcgagg
    24301 agagggaaga gaaattatat ctattgctga agcgcttcag gagaaaaaaa tagcccaaat
    24361 tgccgactta atcacccaga aaaaaggtac ggtacactta gtattaatcg ctggaccctc
    24421 ttcttcgggg aaaaccactt ttgctcgccg tctttatatc cagctccggg ttaacggctg
    24481 gcaaccaata actatttcct tagatgatta ttttctttcc cgcgaggaga taagcgaaga
    24541 agattatgaa aaacctgaag cgttagattt aaaactattt gaagaacaaa taaaagccct
    24601 cattcgggga gaagaagtgg aaatccctcg ctacaatttt atcactggtt ctcgagaacc
    24661 ccggggaagc aaaactcagc tctctcccga tggcattatc atagtagaag gcttacacgc
    24721 tctcaatccc cgattaggag aaaatatccc cggaggagag aaatttaaaa tttatgtgag
    24781 cgccataact caaataaacg ttgatgacca caaccgcatt tctaccaccg attgtcggct
    24841 aatccggcgc ttggtgagag atagccagtt ccggggaagt agaggagaag gagtatttga
    24901 agattggcct cgagtaagag aaggagagga aaaatatatt tttccttatc aagaaaatgc
    24961 tgatattatg ttcaactctt ctttaatata tgagttgagc atcttgagac aatatgctga
    25021 gccccttctg cgggctatta ctcccgaaag ccctcattat ctggaggctt accgcctcta
    25081 tgctttcctc aaccatttta tgcctctcaa ttcctctttc gtcccttcta attccattct
    25141 tagagagttc actggttaaa ctaagctctc ccctttcctt ttttctattt catctagaag
    25201 tattaaagca gagcgcagaa gaattttctc ttcttctccc agggcagggt aaatactctg
    25261 caaagaagag accttgcttt ctaatttttc cagtatattc tcttcagggg gaagagaagg
    25321 agcactaaac actttctttt tccccaaatc tatggtgtct ccgagatgag gaactattgc
    25381 ctctttcccc ttttctttta gagaccgggc aaaagactca gcaatgcttt cttctccgtg
    25441 agttaccaaa aaagtaggac taccctgaaa atactctacc cacttaaaca agtcttcctg
    25501 gtcagcatga acagaaaaac cattaatagt atatatttta gctctaactg ctatttcttc
    25561 tcccagaacc cgcacttttt tagctccttc tatgagagct ctccctaaag ttccttgagc
    25621 ttgaaatcct acaaagataa gagtggtatt ttccttatag aggttgtgct ttaagtggtg
    25681 gacaatcctc cctccggtgc acattccact tccggcaata actatagcct ggtttatatt
    25741 gttgatagct tttgattctg tggaagtgga aacataacgc aggttaggta aagaaaaggg
    25801 ccctttcttt tcccagttga gcttttgaat ttctccagag agaaggttcc ggtaacgttc
    25861 ataaacatca gtaactttgg ctcccagagg gctatcaaaa aacaccggat acttattcct
    25921 tactttttcc ggtaataaag aaagttcata aatcactcgt tgagctcggt ctaccacaaa
    25981 ggaagggatt aaaacttttc ctcccttaga tagtgctcca tctatagctt cagtaaattc
    26041 ccgacgagta tcttctaaac tccggtggtt acgattacca taagtagatt ctattaccac
    26101 aaaatcagct ctctctatta ctggaggaga accttccatt acactataga actgccctaa
    26161 gtctccagaa aaaactactt tgcttccctc tccccatacc tccagtgccg ctcctcccaa
    26221 aatatgggca gaattgcgaa aaacgaattc tatttcccct tcattgatta actcatcata
    26281 acccaccggt acgaataaat tcatggctcc ctctacttct tcttctccaa aaagaggttg
    26341 aactcgaggt ttcccctgcc gaatattttt tcgatttatt ctctcggttt cttctcccat
    26401 aatttttact gtatccagcc atagaagatg cgagagctca atagtaggaa gagtagcata
    26461 aattttcccc cgaaaaccat tacgaaaaag taaaggtatc ctaccagaat ggtctaaatg
    26521 cgcatgagtg agaaggataa aatcaatatc tccgggagaa aaagggaaag gctgataatt
    26581 tctctcctct cccctaccct gcaccatacc acaatccaca agaaaacttt tatctccggt
    26641 tttcaaaaga tagcaagaac cagtaacttc tcgagttgct cctaaaaaag taagctgcaa
    26701 aagatcatct ccttatctat tttcttcttg caaaatttcc cgcaaaaagg ctttgatttc
    26761 tcctcctaca tcttctcgct caagagcaaa agcgatatta gctttaagat aaccaacttt
    26821 atctcctacc ccaaaatgtt tacctcgaag ttccagggca taaatttctt ctttacccaa
    26881 aagagctctt atagcatcag taagttgaat ttctcctcct cttcccggca atgtacatct
    26941 aatagtttca aaaatagaag gaggtaaaat atagcgtcct acaatagcca aagaagaggg
    27001 agctttatct ttttccggtt tttctactaa atcttccacc cgataaagat tctccttcac
    27061 tttttctcct tttatcaccc cccaggaaga gacatcttga ggaggaacag gagtaacagt
    27121 taagtaaact gctggctttt gatgataaag ttctataatt tcttctaaaa aaggggaagg
    27181 ggtaagaaaa atatcatcag cgagaagtag agcaaaaggc tcttctccta cgaattctcg
    27241 agcctgataa actgcatctc ctaaaccccg aggagatttt tgcctcacat aataaatggg
    27301 aaccaggttt cccaccttct ttatttcttt caataattcc tctttccccc gattttccaa
    27361 ttcttgttct aattccaccg aataatcaaa ataatcctcg atagctctct ttcctctccc
    27421 ggtaacaaac aaaatacttt ctatgcccga agccacagct tcttccacca catactgaac
    27481 tgctggctta tctacaatgg gcaacatttc tttaggctga gttttggtag caggtaaaag
    27541 gcgagtccct aaacctgcca caggaataac cgcctttctt acttctttca tgtctttgcc
    27601 tcctcttttc taagatgatg gaccagaaaa agcattaccc ctattcctaa aagataatct
    27661 cctacgctgg ccacaaaaaa acggcggaaa agaaaagaaa aataaggtag catgtctccc
    27721 aaaaaaggta aacgagtgga agaagaaata atggtataat caggataata attattttct
    27781 aaaagctcta caaattgttg aagacccagc caggaagtca cttctaaaga taccggcatt
    27841 ttccctccat tgagagccat aactaaaaaa tttaaaaaag ctccccacaa aaacaagccc
    27901 tcttcccacc ctcttctcct tcctgactta tataaaaaac caatttctat tcactgcatc
    27961 taaagtagcc cgtgctactg cctgataaat atcttctttt accagagttg ccccaccagc
    28021 atttcctctc tctgaggaat attacaatcc acaaaggcta aagctacttc caagtctccc
    28081 aggcgttgaa ttaaaactcc ccgaggggca aacaccacat taccaattct ttgattcaga
    28141 cattctaaag tagcttgaac cactaaagag taacaattac gagaattaga gacactcccc
    28201 gtcaccatac ctcgatagag tttatcactt aactttatct ctacttctac ctggaaagtc
    28261 tggggatttt tcttccaact caacccccac agcacaggcc gcaatccact ctcttttttt
    28321 tcctcttcta aatcaaaagc tacaatgcta attttcttat ggtcaatctc cagattaaag
    28381 cgagcctgga gtaaagtctc tatatcccgg gctaaaagtt tgggaggttt agaaggttta
    28441 gagagaacat gtatctcttt aatctcctcc tctcccacca cttttacatt tattactcct
    28501 tcgatacttc ctatagcttc ttctactgtt ttccaatccc ctaccttaat cacctcttca
    28561 cctcaataaa tgggatcaat taaaccataa ttaccgtctt ctcttttata aactacctgg
    28621 actttatctt tatccaggtc tttaaaaata aagaaagtat gtcctaaaag ctccatttgt
    28681 aaaatagcat cttctatgct catagggcga aggacaaatt ctttaactct ggctacctta
    28741 tccaccggag gtaaggctgg ttcttctact tcttccctct caaattccac taccgcttct
    28801 ttgcgcaata ctcctctctt tcctaaacgc tccttatacc gcttcacctg ccgctccaat
    28861 ttatccataa cctcatccag ggccgaccgc cagtcagaac ctactgcctc tccccggatt
    28921 actataccac tggcggtaag agtagattct actttgattc ttcctcgttc cagagtgaaa
    28981 ttgactgtgg catccataag acggtcaaaa aaacgttcca gcttagaaag ttttttagtt
    29041 acataattcc tcattgtttc actaatttct atgttttttc ctcgcaccac tatgttcatc
    29101 gcctgccgcc tcctgacttt ttattcgttc caccaaaaca tttttctcca gctcttcagc
    29161 tttctccaga tcaaaaatcc ctcctccagg ataaaaagta ttagctaaac cacagctcac
    29221 cgctgcagcg agagaccctt ctatattcct tccttcttct aaatatttta ccataactcc
    29281 caccaaagta tcacctgaac caatagaata gcctcctcct tctgtctctt tcctcattct
    29341 cactttccat atttctcctt ggtaggcagc tactactcct cttttcccgt ccgagagaat
    29401 caccaattct acaccctggt tttgaaaaat ttttaccgct tttaatttat cttcccagga
    29461 agaaatagaa aaacctaaaa cttcctctgt ctccctcgcg ttgggcttta tcataaaagg
    29521 agaggcagat agtgctcgac gtaaaggttc tcctgaggta tctacaaccg aaagagctcc
    29581 cttcctctta gccaccccag tcaaaagagc ataaaaatct aacctcactc cccggggtaa
    29641 agatccagag agcaccaaac aatctccttt ttggagattg ttttctaaaa aacgaagaaa
    29701 agtcaagaca gcagaaacag aaaccaaagg tcctggttca ttaattactg tgtgtctttc
    29761 agttttttct tcgtggataa cagtggtaac tctcgtctca cctgagggcc agttagtgag
    29821 caaaaagcct tcctcttcta aactgttctt aattctttct ccggtacttc ccccctcaaa
    29881 aaggagaaga tgaggattcc ctccttttcg ctttattgct cgagccacat taatcccttt
    29941 tcctcctgcg gtctctacca cttgagaagc tcgatttact tcgttcaaat ttatttcctt
    30001 cataataagc aagcgatcga gagcagggtt agcacaaagg acaaaaatca tagaggatgc
    30061 tcctttaaga agatttccac ctcctcgctg gtgggcaaag ctggaatagc tccttttttg
    30121 gtagtggtca gcgctcctac ggcattagct cggcgagcag cgtgagcgat agcactctcc
    30181 ggaatttcct ttctctctct taaatcttcc cagaacatat aaagggaact gagtagacca
    30241 gctacaaaac catcaccagc accggtagta tccacagtag caacagaaaa tccttccatt
    30301 tctaaccttc gctccccttg cacaatgaaa cagcctcttt tccctaaagt aacagcaaca
    30361 agagctaagg aaaaattctg agagataaat tccagcgctt tttctagatc cgaggtccca
    30421 gctaaaaatc gtgcttcttc ttcattaatt ttcaaaatgt ctaccgattt taaagcctgg
    30481 cgcattcgga aacgagcttc ttctaaatct ctccacagag caggacgaag attagggtca
    30541 aaagataaaa gataaccata ttttttagct aaagaaatag cccgcatagt agcactgtaa
    30601 gccggctcat gagtcatagt aatagagcca aaatgaaaaa ctcctttccc tgaaaagcac
    30661 tcttctccta tttctctctg gtctaagagc atatctgctc ccgggtcacg gtaaaagaca
    30721 aagtctcttt ctccttcctg ggtaagagaa acaaaagcca gagtggtttt ggctttctca
    30781 gtagaaaaga gaaaacgggt gtttacccca tttctttcta agacctctct taaaaattct
    30841 ccaaaggcgt ctttccctac cttaccaata aaataagtat cgattcccag tttagctaaa
    30901 cccaccgcca cattagctgg tgcacctccc ggagctttct caaaaccggg agcatctttc
    30961 aaggtaactc cgtttactgt agaaacgaaa tcaattaaga gttcccctaa acaaactgct
    31021 tttttatcca ctcatcatcc ctcctcctct ataactcctg taaccgggga taaaaatttc
    31081 gcaaagcagg ataaaggtct cgatatagac gataagctct gtcgtaatag tccaccagtt
    31141 ccggggaggg aagaaccgat tcggtgactt tcacagcttt ctctgaggcc tcttccaccg
    31201 aagaaaatac tcctctccct accgctccta aaagagcagc accaaaagct ggaccttcat
    31261 caatcttgac cccgaaaaga ggaactccat aaatagaagc ctgaattttt ctccatagag
    31321 cgctgcgtgc tcctccacca atagctctaa cttcttgaat aggaatattc atctctctca
    31381 taatctcaaa ggaatctcgc ataccaaaag taactccctc catcactgct cttatcatct
    31441 cgtttttacg gtgtttcaga gtaagcccaa agaatacccc ccgagcataa gggtcagcat
    31501 gaggagtgcg ttctccagta agataaggaa ggaaaatcaa tccctccgcc ccaggaagag
    31561 ctttttccgc ttcagcagtc aaaaggtcat agctatcttt ttctaaaaga gaagctaagt
    31621 ttttttcctc catacccagc ctatctctaa accagcgtag cgacccaccg gcagaaagca
    31681 tcactcccat cacatgccat ttaccgggaa ccgcatgaca gaaagtatgc actcttccct
    31741 ttttatccac tttcacctca tcactgaagg caaaaactac ccctgaagtt cctaaacttg
    31801 ccgaaactaa acccggcttt acaatacctg tgcctattgc tccagcagca ttatcccctc
    31861 ctcctgctac tactaaaact tcatctccca tcccccacat ttccgccact tcttttttta
    31921 agtatccggt aatttccggg gattcaaaag aagggggaaa ccaagaggga ggaaacccca
    31981 gaagagaaat tatttcttct gaccacttgc ggcggggcac atcaaagaga gaagttcctg
    32041 aagcatcaga aacatcagtg gcaaaatccc cggttaaaag aaagcgaata taatccttag
    32101 gaagaagaac cttggctact ctttcataca agtgtggttc attatttttt aaccacaaaa
    32161 tcttgggggc agtaaacccg ggaagaacag ggttacagtt aatgctcatc aatttttctt
    32221 ctcccactat ccgagtaatt tcttcacatt cccgggcggt gcgctggtca caccagagaa
    32281 ttgctggacg taaaacttct cccttttcat ctagaaatac tgaaccgtgc atctgtccgg
    32341 taagccctac cccttttact aaatgagggt taatctctgc tgcttgcaaa gcctttttta
    32401 ctgcctgaac cgttccccgc caccagtcag cagggtcttg ctctgcccag ttaggataag
    32461 gagtatagag aggatattcc tccacagcct tggacaccaa ttccccttct tcattcacca
    32521 aaactacttt acatcctgta gttcccacat caattcccaa aaagtattcc atcctcattc
    32581 ctcctcagac aattaaagtg aaaggctctt caagaagatt ctttaaaaat tgcataaatt
    32641 gagcagctaa agctccgtca attaaccgat ggtcgcaaga gaggctcaac tcccacaagt
    32701 aagcaggaac aatctcttct ccattgaaga aaggtttttt acggattgct ccacaggcca
    32761 gaatagctgc ttccggggga ttgatgatgg cagtaaaaga atccaccccc cacatcccta
    32821 agttagaaac agtgaatact cccccggaaa tttcctccgg agttaattga ttagattttg
    32881 ctttttgagc taaagtattg atttcttgag taatctggga tattcctttt tggttagctc
    32941 tcttcactac cgggacaacc aatccctctt ccagtgctac tgctactcca acattcactt
    33001 ccgggcttat ttctatttct tctcccacga gatgagcgcg aaagagagga aatttctcca
    33061 gtgctttagc taccattttt accagaatat cggtgtagga aactcgtaaa ccagtttcct
    33121 tttctacccg aggaagaatt ttttctctca tctttactaa ttcctcggca taaatttcct
    33181 gatgaagaaa aaaatgaggg gtgctttgat agctctcagt taagcgctga gctataattt
    33241 ttctcattcg agatagaggt atgtgctctt tttctctcgg agcttttact tcttctatta
    33301 tcaatttctt ctctttttcc tctatagctt tctctatgtc ttcttttaca attcttcctc
    33361 ccggaccact accccgtatc tgagaaaggt caattccatg ctcttcggcc aggcgacgag
    33421 ccaggggaga aactctaact ctttcggttt ccagagtaag aggagcttct tctcgttttt
    33481 cttctacttt tacttcttcc tttgtttcta ttttctcttc cagtttttcc gtctcttcct
    33541 caggaattgc ctctccttct tttcctatat aagctactgt ctcagtgatg ggaacagtag
    33601 ctccttcagg ataaagaatt tttaatacca ctccttcagc aggagattct acctccatat
    33661 taaccttatc agtagccacc tcaaacagaa catctcctct acttactcgg tctccttctt
    33721 ttactaacca tttatttatt actccttctt ccatagttaa acctaacttg ggcataatta
    33781 cttttttagc catttacgac actcctttct tgcaccaggt tcatcaattt ttccacgtcc
    33841 actctatagg gtccggtatc ttctaatcta atttcccaat cttccccttc ccgcaaaact
    33901 atttctcgct ccccgtccaa agccataact cctctttggt gtggtagaag aaaagagaca
    33961 cccagaggca aaaaatccca tttccgaatt tttacctctc ctactaaacc cggagctaaa
    34021 ggagctctaa ctattttccc tccctctcct aactctacca tcgcccctct ttcttcttct
    34081 gcactaatac tggctaacct tcccaccacc gagcttaaac caattttcaa cggactgctc
    34141 tgagttacta ctatcaaacg aatcaattcc ggttcccaca ctgctcttgc ccccaccaag
    34201 gtcttttcta ccaccactgc atcaataaga gctatttctt ctttgcctcc ctctcttggc
    34261 tttgctttca gtattttact ttttttcacc actttttctc gcagagaatc attttccaga
    34321 aacaacccta ctgccatacc cataaccgta ggctcaatat tttcagcaaa tacgttatta
    34381 gtacctccaa aaagagagaa caggggagta agagcacttt ttttagccac tattcgatta
    34441 gtcccatctc cccctattac caccagagca tccacttttt tctcatttac cgcccaatcg
    34501 gtgaagttta aagtatcagt ttcatcaccg aaaaccgcaa taggagcgca acgaaatatg
    34561 atctccggag aagaaaagct ctttttcact atttctacta aatcataagg gtcgggcata
    34621 taggctacct caagcgggct attcaccacg ctctctatac cccgcaataa gcgaagagta
    34681 taattaattt tttcctggtt gccaaaaacc gaaccataag ctattaatcg ccgaatatct
    34741 ttccctgctg cagggttaac cactaatcca attttattca gttcagtgaa cctcctgtcc
    34801 tccagtaacg ttaacagctt ggccggtcat atagtcagaa taagaagagg ccaaaaacac
    34861 tgccacatta gctacgtctt ctactgtgca ttctctacct aagggaactt tttccacata
    34921 tatttttctc acctcctggg gaggaactcc taatttctta gaatattctt tatccagaag
    34981 gtcccacatc ggagtagaaa aaacgttacc cggacaaatg gcgttaacat taattttata
    35041 aggtgctaag tccattgcta cgctctgcgt taaaccaatt ataccaaatt tggaagccga
    35101 ataagcacct aaccacagcc ctcctttttt acccgactta gagctgacac tgattatttt
    35161 cccactcttt tggggaatca tatatttagc tacctctctg atacagagaa aagttccctt
    35221 taaatttact gcaattatat tatcccagac ctcttcagga acatcagtta taaaatgagc
    35281 acgaataatt cccgccgagt ttaccaggat atcgatttta ccaaattgat taaaggttgc
    35341 ttgtgcagca tcttttactt cctcactttt agtaacattt acctgaaaag ctaaagactt
    35401 tcttcccact ttttctattt cctggacggt ggcttttgct ccttctaagt tcatatccca
    35461 gcaacataca tctgctcctt cttctgcaaa acgaagggca ataccttttc caataccgga
    35521 cgccgaacca gtcactatgg ctactttgtg agccaaaggc tgaggatcca ttttcttcac
    35581 ctcttcttag ttatttttcc atagatacaa ccgctttaag agccacaccc tggcgcacta
    35641 aatcaatacc ttttaccact tcttctaaac tcaaaacatg ggtgataaga cgtttagctc
    35701 taacccgttt actataaatc agtttagccg cttgttcata ttgagaagca tgagaagcaa
    35761 aaactccaaa aactcccact tctttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    35821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    35881 nnnnncctgc tccctggctt cgagccaatt ccacatgcat acagccaatg ggacctgctc
    35941 caataactaa aacattttcc ccgaagccaa tgtgcaaata agattgaccg ttaactacac
    36001 aagatagagg ctccactaaa ctggcttcta aaaggtcagc gtcaggagga agaactaaga
    36061 gatttcctcc ctctactcct tctcgaggga tttttaaaaa ttcggcaaac cctcccgggt
    36121 aatcatagcc gatgtatttt tgctctggac ataaattttg caaaccccgc cgacagtaag
    36181 agcagcggcc acaggaaata acagtgataa ctgacacttt atctcctact ttatacccct
    36241 ctactccttt tcctacctct acaatttccc cggctatttc atgaccggtg gtctgaggag
    36301 gatgaacatg aggatgacca tgatggaaaa ttcgcacatc agttccgcat atagcacaag
    36361 ctgccacccg aaggagtact tccccctctt ccactcgagg aacttccact tccctaattt
    36421 ccattttttc cggacccagg aaaaaagcag ctttcaagct tatctctctc ctttccagat
    36481 gtgatttttt agagctggat aaattctctt atacacctca aacttctctt tatataacac
    36541 gtttctctct ttatctggtt cataaacctc tctaacctgc actaactgat taattaaacc
    36601 agcaaaatct tctattttct ctctcgcctt cctccctaaa agagccgccc ctaaagaagc
    36661 tgcctcagta acagacagag tctctaccgg taacccatag acatcagcct ttatctgtaa
    36721 ccaggcaggg cttttggctc ctcctcccac tgctcgtaac ctttccactt ttactcctgc
    36781 ctgagagaga agttccaggt taaatttcat ctcatagctt actccctcaa gaagtgcttt
    36841 gatgaattct tctcgagaag tctctaactt aagccccaca attactccgc cagactgagt
    36901 atcaaagtaa ggagtcccag tagtcgtaaa atggggtaaa acccagatat tggtgggctg
    36961 agagggaaga cgagagagaa tgaggtcgta tacatcacaa ttttccctct ctgctatttc
    37021 tttttctttt tgagcaaaat tatctcggaa ccagcgcaga agtgctcctc ccgtaaaatt
    37081 ataaaccatg gtaagataaa gagaaggaaa agcatggtgg taacaacaaa ggttattttc
    37141 ttccataacc gggctgagaa ctaattcccc cagagcagga gctatgcatt ctaccgttcc
    37201 cgtggcatcc atagccagag ctggtttaat cactcctgaa cctaaagctc cacagggctg
    37261 gtcgtgaccc cccgttgcta ctataacttt ttctttccat cccatctttt gagcaaagga
    37321 agaagttact tctcccacta cttccccaga aggaagagga cgagctaaac gcatggggtc
    37381 aatccccgct aaggaaagga tttcttctga ccacttttct ttttggacct caaacatcat
    37441 agttctcccc gccagggaaa aactaatggc tggctccacc cccaagcggt acattataaa
    37501 atcttcaaaa cataaaaaat agcgagcttt ctcaaataca tcctttttat attttttcca
    37561 ccatagtatt ttgttcactg ttccaatccc ggaaagagaa gttccggtaa gctcaaaaaa
    37621 gcgtcttcga ccaattttgt cttcccagaa aggaacaaat tcatctcccc gctggtcaaa
    37681 agtaacaata ctgcgcgcta aacaatctcc tttttctcct atcggaacta ctgcctctcc
    37741 ttgagaagag atggccaggc tgcgaggagg gtcatttcgt aaatcttggg acacttcttg
    37801 caagctatct tctactgctc ggtaaacctc ttcagcatct aattctatcc accccgtctg
    37861 tgggtaataa agagggtact ctcgatagga acgggcaata atctccccac cttcagtaaa
    37921 agccactact ttgcaacccg tggttcctac atctacaccc aggtagctca aagcctctcc
    37981 tcctctctaa agtaagctct ttatatactg aagagccgat aaggcatccg cctctttctg
    38041 cattgtttcc agttttttcc tatctatttt ctggattatt tctaaaaagt aatttatcat
    38101 ctttatactg aattggcagg ccaaagccgg gtcttctcga taggggaaaa tatccaatcc
    38161 taaccatcct tcataattag aaaggtaaag gtagtaaatg aattctaaag tttcccagaa
    38221 atggacgctt cctactgcca tatcatcgtc ccaatcccgg aaattatcgt tgagatgcaa
    38281 ggcgaaaagc ttaccgtatt tatctaaaag agctactaca ttggctggcg actccttaac
    38341 attaaaggaa tgcccaagat caatggttac tcctacattc tggcctactg tttgagataa
    38401 gaacaaggcc agtccagcat tagctatggt agagtgggta cgtggctccc gcagtttata
    38461 ttctaaactc aatttcaccc ggggattgtg gctggctatc tcagaaagcg cattcaccaa
    38521 aaggtcccat tgagctcggt aattaatttg aaaaggatag tcaaaaccat cttgcccggg
    38581 ccagagattc atcacatccg cccctaaata ctctgctatg tccatagctt ttttgctcat
    38641 ctctatagct tttttcctta tctctttttc acacatcaac cctccccagg aaaactccgg
    38701 actactaaag agatctacat cacaggaagc caattgcagt cctctttttt ctatctcctt
    38761 tttcacatat tcagcattat tttcattgag aggtgcagga tagtgcatca ttaatcctcg
    38821 caaaccctct atcgaggcaa ttttgtccag agattcttct aaggaaactt cctttttgta
    38881 gcctttagta caaaaacggt ctggacaact accgaacacc cacactccag tggcaaaacc
    38941 tttacttttc atattagata aacctcctct ttatttaagt tttggaaaaa tccgaaaatt
    39001 tctggcagtt ttgaactaaa taagtatcac cctaaccctc tccccccact gagggatgct
    39061 ttttccctca aagtgttttt tgtttttaag tctttatttt tctttgtttt gggggtcatt
    39121 agggggtatt ttccccctaa tagtagaaag atgcttttct ttcctcagtt agtttttttg
    39181 tgttttattt ttgagttttt atgtttccaa gggttataga gggagttttt tctcccccta
    39241 tagttcgggg ttataaggag gtatccccca ctgaggggtg ctttctccct cagtagttct
    39301 ttactctttt aagtttttat cttctactgt ttttttgctt tagtccttct gggggttatg
    39361 aggaggcagc gcccctttat agtcttttat tcacttttca aggtgcgctt aaatttctct
    39421 ttaagtctca agagcctgca aacttaccta tattttactt ccgtctctct gtaagagagg
    39481 gattttctat aacaatttac tgatttcctc atttatttcc cttactgctc cataaatttt
    39541 ctgataaatt ttatatctaa gttggtaggc ctctttttta tctggagagg gagaaacctc
    39601 tttcccaaat cttaccatgg ccgctatagc ttcatccaga ttagaaaata tacctcgggc
    39661 tttccctcct aacatggcgc ttcccaaagc cactgcctct tcagtaaaaa gggggaagag
    39721 aggcttttgt aaaatatcgg ctcgaatctg aagccagcgg gtagagcgag ctcctcctcc
    39781 tactgctcgc aagttgtaca gggggatacc aaactcttcc cataaatcca gattgatttt
    39841 catctcatag gtaagacttt ccaagatagt tctaactatt tctcctttgc tcgcttcaaa
    39901 agtcaagcct agaagtgttc cgcgagagta gccatcgaga tagggagtcc cggaaccagc
    39961 caagtgaggc aaaagaaaaa gagaggaagg atgggaagga atggtatcaa tcaaaacttg
    40021 ataggggtct ttgccttcct tttttgctat ttccttttcc tcgccgccga aggtatcccg
    40081 aaaccagcgc aaaaccgaac cacctgaggc gatataagaa agagtgaggt aggaatcttt
    40141 caccacatga ggataacagc aaaaaccatt ttccatcatc tcttgagtga ggcgaggttt
    40201 ttctcccaga tttaaagcca cacaatctga agtccccatc ccatacattg cttctccttc
    40261 ttttctcacc ccacagccta aaactccgca aggctggtca tgacctccgg tcgctaccat
    40321 aactatagat gataaaccta actcctgagc taaagaagga cgtagatggc ccacttcttc
    40381 tccagagggg gaaaggcgag gaagtttatc tttgtctaaa gaggcaaggt gtaaaatttc
    40441 atctgaccac tttttatgta ccacatccag catcatagta cgagcagcta aagaataatc
    40501 aatgaccggc tcttcaccag tgaggcgatg gagaacaaaa tcttccacac agcaaaattt
    40561 atctactttc tcaaaaatat ccggtttttc atctctgagc cagagaattt tattcacggt
    40621 atacattccg tgaaggggca taccggtaat ttgaaaaatc tggaaagggt ccaggcgctc
    40681 tttccagaaa tctacgtatc tctccgaccg ggtatcaaag ttggctattg cccgatagag
    40741 aacttgacca tctctaccca gaggaatgag agtctcaccg tgagaattta ctgctatcgc
    40801 atctatggta tctcctgttt gtgctgctac tttttttaaa gaatttagca ctgcttccca
    40861 gattttatcc gggtccaatt ctaaccatcc ggaacgagga gttaaaaaag gatattcttc
    40921 ataaccagaa gcaataatct ccccaccttc agtaaaagct actactttac aaccactcgt
    40981 ccctacatct atccccacca aagccaattt cttcacctcc ttccctgaag taagaagaat
    41041 gaaaaaccag ccataaaaag agaaccaaaa cccaaaccac ccagaaaata taagaaagtc
    41101 cccatttttc tcctataact cctcccaacc agggaaagaa catcgcccct acgcctccaa
    41161 aggctaccac aaaaccgata gcctcctctt ttctttctcc cattttttgt gctacccagg
    41221 ctactatcaa aggccagata acagaatagc caaaccccac tcctcctaag gctaaaatag
    41281 cccagtgtac ctcatgagta aagttgagaa aaaggagaaa aattaagctc aaaaaggaag
    41341 aaaaacgcaa aataactgga taggaaagag aacgggaaag aaaagcaaaa acaaatctcc
    41401 ctatagttaa ggcgctccag aaaactgcca gagctattcc ccccgaggat aagctacctc
    41461 ccaggccttt aaccaaataa gtagctatcc aagaagaaga acctatttct gctcccacat
    41521 aaagaaacat cgctaaaaaa ctcaccacta taaagccatt acctctcggt ccaaaaagac
    41581 gactttcttc ttttatttct ttttcctgag gaaatttttc cttactcaaa agagcaaaaa
    41641 tcaaaaagga gataattccg gggaaaagat agaataatct ccaggaaaag cccctttcta
    41701 ataaaaatcc ggccagaaat ggagcactgg ctgcccccag accaaaagaa atctgagata
    41761 gattgagagc atagccttct tctcctggaa acatttgaga aataagagaa gtaaaaggag
    41821 cttcacaaag tccccctcct atccctaaga gcaacaatcc cccttctatc aaggaaaaag
    41881 aaggagaaac actaacagag aaaaaccctc cagctaaaaa agcttgtccc aaaagcaaaa
    41941 ctaccttttt accccagcgc tgagagagca aaccgctact aaaagtaacc acggtataag
    42001 ggagataata aaaggaaaag agccgccctg ctcggccggc cgatacagaa aactcatcca
    42061 tccaccgggg gataactgcc ccggtgatga tcatagaaac agcgagaagg taaaaggcta
    42121 agaaaataat taaatataat cgagctttac tttccataga aatgagccag agcttctctc
    42181 cagtaatcca cactcttttt catactatct aaagcactct catcactaaa ggcatgggca
    42241 gaaaagaaaa actcaaatac taaaaccact tcttttgccc ctgaagcttc aatagcttcc
    42301 aaaacccgct cgggaacgat tactccttga gcattatttt cttcggtaaa agtccagtgt
    42361 cgacttcctt tccggtcggt ctgttgcata tgaatggaag gggaaagatg agccagctct
    42421 cgaagccagg agtaataatc caggtcttta gggtcagagg agcgataata accatgcccc
    42481 acatcgagac acaaaagtac cggaatatgg gactttttat tcactcgctc ccacagttcc
    42541 ttagtttcat cgatggtaga aggcatctcc cggggaagag acataggctc ccacagaaga
    42601 tatttctgcc ctgcttcccg agcaacaaaa gttaaataag caatttcttc tataagcacc
    42661 tcagttagat atgcttttct ctcggggtta tttacatctt tgttgctcaa acttcccata
    42721 taaatccctg aaccctcagc tcctaaaaga gaagcagccc gaaccatttt ttcataccac
    42781 cgcagatacg agcgacgcat cccggggtcg gggtctaaaa gtaaattgaa tttgtgagct
    42841 gcctctcccg taactgcagt gtctattttt accccctgtt cctcagctaa gcggcggata
    42901 aaagcgcatt tttcttttac tatctcctca tcaatgatta ccgggtctaa aaggtcaaaa
    42961 gaaaactgga catgttgtaa acccagctct tttttgacta tccgtagcca ttcttcaggc
    43021 tccggatagc ggcctaaagc aaaacaattg ttaatgccca actttacttt agccatacca
    43081 ctacctcctc actaaatttt tcactgcctc aactatagtt tctggtcggg gaatggagtt
    43141 atcctctaaa tatctactac aaggaactgg agcatctgct gcacctaagc gtactatagg
    43201 agcatctaag tagtcaaaaa gctcttcctg aattttcatt cctatctctg cccctgctcc
    43261 tccggtttta catccttcat gaacgataac cacccggtta gtttttcgaa ctgattgagc
    43321 aatagtttcc atatccaagg gcacaagagt gcgagggtct atcacttcta cctcaattcc
    43381 ctcctgagag agagcctctg ctgcctgaag agcaaaaagg agcattcgag aataggcaat
    43441 aatagttgca tctttccctt ctcttttcac atctgctact cccagaggga ttatatattc
    43501 ttcaggagga acaagacctt gactgttata aatcattttg tgctcaatga agagtaccgg
    43561 attatcttct cggatggcag ctttaagtaa acctttggca tcaaaaggag tagcaggcat
    43621 caccacttta agacctggag tatgcaagaa ccaggcctcc agactttgag catggtgagc
    43681 acctgaggag cgaccacatc caccttcggt gcgaataacc ataggaaccc gggcttttcc
    43741 gccgaacata taccgaattt tagctccttg atttactaat tggtccatac ataaaccgac
    43801 aaaatcaata tacatgattt cccctaccgg ccgcaaacca gtaatggaag agcctacaca
    43861 acagcccaca atggctgctt ccgatatagg agtattgcgg actctctctc cgccaaattc
    43921 ctgccataac cctcgagtta ccccataagc tcccccatag agagcaatat cttctcccaa
    43981 taatactaca ttttcatctc gcttcaattc ttcttttagc gcttcattga gcgcttcccg
    44041 ataggttatg gttctcattt ctcccttcca ttcactgatt cctctaacta ctttagttcc
    44101 tttccttcgc tctaattcgg caaaatcctc ttctacgtat acgtctttag taatttcttc
    44161 cagcggagga tagggacttt tctcggcaaa atctactgcc tcttctactt ctttttctac
    44221 ctctgcatca atattcttta tctcttcttc cgttgctact ccattatcca ataaccattt
    44281 cttatatcgt tttatagcgt ctctttcttt ccattgattt tcttcttctt tagtccgata
    44341 agcccggggg tcgcttttag aatgccctaa atagcgataa gttttacatt caattaaagt
    44401 gggaccatcc cctcgccggg ctcttttggc tgcttcttcc actgcttctt ttaccgctaa
    44461 aacatccata ccatctacta ttactccagg cattccataa gattgagctc tttctacgat
    44521 atcttgaatg ggaaaagcct ctttaactgg cacagacata gcataaaggt tattctcaca
    44581 gacaaatact actggtagtt tccacacccc agctaaattt aaggcttcgt gaaaagctcc
    44641 ggtattggct gctccatcac cgaaaaaaca tactgttact tttcccgtgt ttttatattt
    44701 agcagttaat cctgagccca cagcaatggg aatccctcca cccacaatgc cattggctcc
    44761 taagtttcca gcattaaggt cagcaatatg taaagatccc cctttacctt tacaatatcc
    44821 agtaatttta ccaaagagct cagccatcat cttatccaag gtagcccctt tagcaataca
    44881 atgtccatgg ccgcggtgag tggaagtaat ataatcatcg ggattaatag cattacaagc
    44941 tcccaccgcc acggcttctt ctccaatata aagatgagta gtacctctaa tcctatcctg
    45001 gctaaaaaga tcactcaccg tttcttcaaa gcggcgaatt cgcaccatat cccgatacat
    45061 gttaagaaat ttgtctttag accccatctc tatcctccta ttccaggttt tactactatt
    45121 tttaaataat ttccactttc cgcttcctca atcgcttgag gaagctcatc taaacttacc
    45181 actttactga ttaatttttt cccttctact ctccctgccg ccatcatttc taaagcccag
    45241 gtattatgaa gaggagtaga gccagaagtt ccctgaatca aaagttcttt ataatgaatg
    45301 aggttactgt ctaattgagc tattttatta tctcccggaa gacctccaaa gaaattaatt
    45361 cgccctcgag cagcagccaa atccagagct tcttcttggg cttttcgggc tggagcagca
    45421 acaattatca ccgtggggcc aacaccttcg gtaacctgaa gaagcttttc ctttaagttt
    45481 tctttttccg gattaatata atagtcagct ccaaactccc gggacattaa aagtctcttt
    45541 tcttgaactt ctgaaactat aactttgctc gctcctaaag cacgagagag cgttatatgc
    45601 atacatccca ccggacctgc tcccactact aaaacatagt ctcctactcc tacttgagag
    45661 agcagctctc cattcaaagc acaggccaga ggctcagtta agctggcttc ttcaaaagat
    45721 aagttatcag gaatacggtt gatgtttcct cctcgtagcg ctcgctctgg aactaccata
    45781 tattcagcaa atgccccagg atgatgatac cccagagctt caaagtttaa acataggtta
    45841 ggtaatcctc tccggcaata aaaacattct ccacagtgca atataggagc tacggctacc
    45901 ctctctccct ctttatatcc attcactcct tctcctaaag cagcaatcac tccggcaatc
    45961 tcatgcccgg ttatctgggg cggatgaata ttgtgatggc catgacgaaa aatacgcaca
    46021 tcagttccac aaatagcaca ggcttccact ttaagaagaa tctctctccc tttcaaggaa
    46081 ggcacaggac gctcctctat ccttatgtca cctggcccat aaaacaccgc cgctttcatt
    46141 tatttccact ccttcctttc tcgagaaaaa tttttataag cagaaagaat ggcttgacct
    46201 aacttgctct ctgcttttgc tccacatatc tcctctataa ccttctctgc ccccttctcc
    46261 tgaatcattt tttgcagttt taccgcttct tcatcccgag gacagtcgta ggaaagagcc
    46321 gccccgcaag ctatagctat atagtgggga aaaatctctt gggaaagaca caaatgcgct
    46381 cctcctatta gacggtcatc gggtgccaat tttcggagag gctcccttcc tacccgccct
    46441 aaggtatcta aaagcaaagg attggcaaat ctttctcgta aatcatccac caattcctga
    46501 tgctccctac ggtttaaatc gggatatttc cgcaagagag cctgagaaac ttcttcccag
    46561 gctccatcga gaacctttct tatttcctca tcttttatgg cttcatggat ataggtataa
    46621 cctttgtggt atcccaaata agctaaagta gcatgaccta aattatggat aaataatttt
    46681 ctatccactt ctgctggaaa attgcttgcc gctttcaacc cggttatttc tggtacttct
    46741 ccttttactg cctgagcatc aaaaggcaag tggtggtaag attccgctac cacaaaaaga
    46801 gggtcttcta ctccgtaacg ctctcccata accggcacca tccgagctac cactgttccc
    46861 actaacccca ctttttcttc taaaaatttt ttaccggcgg gagaaagata tttttctatt
    46921 tctcccctca agagctgtgg agcatctttt aagttctcac aaagaaaaat atttaaaaaa
    46981 cctccgtcct gagagcgaag ctctaaccct tgagctaaaa gaggagcgat aaagggaaga
    47041 ttttttactc ctacagcagt aaatactaca tctccttctt ttatttcttt agctacttta
    47101 tctttttcct gagtagaaag ggctctgagg ttatctatta ttaaatcctc ttctttccca
    47161 tcttttcgga gaagacgcaa aggataagat ttccgttcat tcaataatcg agccaggtct
    47221 tctctggctt ccacaaaaat aatctcaaaa cctgattccc agagcaagtg gccaatgaac
    47281 ccccgtccaa tgttacctgc tccaaattgt attccttttt tcatctatgt tttccttcgg
    47341 cttcttcaat aaaagctaag gtttcttcta aacttcccac cccttcggtg gctcccattt
    47401 ttctaacaca catagctcca caggcattgg caaaacgccc cgtttcatag aggtcccagc
    47461 ctcgcaggta tcctactaaa aacccggcac aaaaagcatc acctgctcct gtcccatcta
    47521 ctgctactac cgaaaaggga gggactaaaa ctttttcctc ttcagtcata ataaaacttc
    47581 cctcttcccc cattttaata gccactacct ttattccata attcataaaa aactgagtga
    47641 tatcaggaag attagttttt cctgatatat ggtgagcctc atcacgattg gagagaagaa
    47701 tatcaatata gggaagaaca ggctcaatgg tagaaaacca ttttcccgta tcatcccagg
    47761 cagtatctaa tgaagtaata acccccaaat ttttggctct tcgcaatact ttagccgttt
    47821 cttctccatc aaaaccaggc atgagaaagc ttcccgctat atgcactacc tgcgagctac
    47881 ttaaaaaact atcggctata tctttttctc gcaaaccccg gttggctccc ggataatgaa
    47941 gaaaagtccg ctctccctgg ccatctacta ttaccatggt agctgaagta tgattctcat
    48001 cgtctttcga gattcccccg gtattaattc catgcgctac cagagaattt atcataaatt
    48061 ctcccagacc atctctcccc acttttccca ctacttttac ttcttctccc aattttttca
    48121 aatccactcc ggtattgtgg gcacatcccc cggcgtggag ttccatatcc tccactaaca
    48181 ttagccttcc cttttctgga aagcggtcaa taggacgtcc taccagatcg gctactagta
    48241 tccctatgca ggttatttca ctcatctttt ctcccccttt tctatgattt ttaaagctgt
    48301 cccctcatcg gtgataagaa tattgataaa gccacctcgc aaagctccta agatggcttc
    48361 cactttatta tctcctccgg caataccaat tacatgaggc atatggcgaa tatcttctaa
    48421 gggaatagcc acagttctct gatgcagggg agtatcaatg atttctcctt cctgattata
    48481 aaactgaccc actatatctc cgactgctcc tttttgaatt aaaagttccc actcatgata
    48541 gtcaataaaa ccggttaaag cataaataga gtaatgaggg tcaactgctc caatacctaa
    48601 aagagaccat ttagcctgac gagccaggtt gagagtcttt tcattaattc gttctcgcag
    48661 ataaaaatcg cgaatctctt ctgtttctac caccgctgga gcataaagat aataacactc
    48721 tccctcaaaa atagaagcta atctctcccc aatggtataa ggattgagcg aagctgaagc
    48781 tgttagccct cccactaggg tgacaaactt gagattttta actgaaactg atttgataga
    48841 taaagccacc tgttttaagg tcctacccca ggcgataccc actagatctc catcgcttag
    48901 aactctctcc aggtaccagg ctcctgcttg tcctaaagtc tccagcaaat ctcgctgagg
    48961 agaaacaggg actaccacgc tatcctcgag ggaagggaaa agttttcgca gttttttctc
    49021 tactaaaaga caattagcta aaggagactc gatagaaatt tttactatcc ccgttttttg
    49081 tgcccggtct atgagacgag ctactttggg ctgagaaatg tttagatgac gagcaatttc
    49141 tcgctgagtc attccttcca taaaatgaag ccaggcaacg cgggtaacga actccacatc
    49201 tgaattattc attttctttc cttttcgaat tgatattcaa aaatagaata atctttcaat
    49261 aacattgtac aataaaattg gatttaatca agaatctcac gctaaaaatt aacctttata
    49321 tttcaaacct accgggggaa ataaacacta acatttttaa gataatatct cgtgcaggtt
    49381 ttgcagaagt aaaatatgtt ttttgcttct aaagaataaa atttcatttc ccggaagcga
    49441 gaactaaaac cagaacttcc tttgcccctc ccctctttaa tgtctggctt aagctatcta
    49501 cagtagagcg agaggttaaa acatcatcca ctatgagaac ccgtttgttc tgaaaaaggt
    49561 tattctcctt tatttcaaaa gcccctcgca tattttttct tctttcttta accttcaaac
    49621 ctacctgagg taaagtgggc tttattcttc taacttcttt agaaacaatg gggatggaga
    49681 aacatttttt aatctcctcc gcaaggataa gtgattgatt aaatcctcgt tctttaagac
    49741 gagcagggtg aagaggaaca ggtagaagac aatccacctc cttcaaaaag ggattttttt
    49801 ctaaatattc acaaagaagt tcgccaaaga gatagcgaaa agaggtaaat ccttgatatt
    49861 taaaagcctg aatacagtcc cgtaaaggag aagtgtaaag cgctaccgcc cgagcagtgt
    49921 caaaatggaa aggaaaagtt cggcagcggt gacacaaaag agtggaaggg ccaggaagag
    49981 gcctaccaca cttcacacac aagggactgt taagatactc aatccgggaa aaacactcag
    50041 gacacagagg aaaaccctgg gagcaagaaa tatatgagcc acaaaccaga caatgctgag
    50101 ggagaaagaa attaaaaatg ccttctaaaa ttcccctacc ttcttcatct tccatattct
    50161 ctccccccag agcaacaatt gaagtcattt ccttgaatta taacagaatt cgccattatt
    50221 tagctttctt ttggacttat cttaaaattg ctttattata ggtaaccgca aaaatccatt
    50281 acaataacta taaggggaca agccttctta taatctaatc atgtcccaca attgctttct
    50341 ggacactatt tatgattaaa cc
//
LOCUS       DAOSVE010000002        49091 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_500_length_49091_cov_73.888490, whole genome shotgun sequence.
ACCESSION   DAOSVE010000002 DAOSVE010000000
VERSION     DAOSVE010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 49091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49091
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_500_length_49091_cov_73.888490"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            254..640
                     /locus_tag="PK016_00245"
     CDS             254..640
                     /locus_tag="PK016_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009710690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HXL01542.1"
                     /translation="MTFYRVGWYTILKQKISEVKKMEEINNHEGNSLEDSRHRLWDDE
                     KVVKEILVRLNRIEGQVRGIKRMVEEEAYCDDILNQIASIQSALYGVARLLLEKHMKS
                     CVKEQILSGDERVIDEVLATIFRMMK"
     gene            661..3060
                     /locus_tag="PK016_00250"
     CDS             661..3060
                     /locus_tag="PK016_00250"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HXL01543.1"
                     /translation="MEEEKEKNNQILTIPISGMKCAACASRVEKGLAEIPGIHSVNVN
                     LASGKATVGYDVGKVDFQDIVDKVSQIGYEVPEEEIELLIEGMSCSSCAARIEKKLNT
                     LPGVKRAAVNLASNKAYIQFVPGIINQAEIEESIESLGYGVQTPSRALSERGKGRKRN
                     RENWQKIRFLAALFLSFPLFYMMVANWVGKGQFMIDPWIQLLLATPVQFFSGWPFYRG
                     AYHSLKSGSASMDVLVALGTSVAYFYSLISLVAGWGVFYFESSAMLITIILLGKLLEE
                     RAKGKASEAIEKLVKLQPEVAHVLQNEMEKDIPAERVKVGDMVIVRPGERIPVDGVIV
                     EGNSAVDESLLTGESMPVEKHPGCEVIGGSINREGSFIFRATKVGSRMVLARIVKMVE
                     EAQGSKAPLERLADRVSAVFVPVIIALSVITFLGWYIEGAGFANALMHMVTVLVVACP
                     CALGLATPTAVMVGIGLGAEKGIFFRGGEYLEGIEKVNTFLLDKTGTVTWGTPSVDDF
                     FIPSLFDVNETWAIIASGEKRSEHPLGQALNKYGEKLGVPNREIKNFEALPGKGIRFQ
                     YQDEVWYIGTEELLKSQGVDLTEVAEYKKRWEREGKTVVLAATRGKVRAVIAVVDTIR
                     DEAREVVSELKRMRLEVAMVTGDHEIVAQAIAQKAGIDKVFSEIMPDEKVEIVRKLKE
                     EGKVVAMVGDGINDAPALAQADVGIAIGTGTDIAIESADVILMNGELKSLISALRLSQ
                     FTRRKIKQNLVWAFVYNLITVPLAMFGVFTPVMGGTAMALSSVSVVTNSLLLKRYRNK
                     I"
     gene            3225..3677
                     /locus_tag="PK016_00255"
     CDS             3225..3677
                     /locus_tag="PK016_00255"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HXL01544.1"
                     /translation="MNKNRGVTFNFFRSWRLRWQIASLEKEREESLLRLGKKVYELFV
                     ERKNWGKEEVRENCQEVLSIEEEIEDLEENIRRLGGNKPRRVCPSCGEYIEESSLYCP
                     RCGFYQKQELTCSQCGEKINPQDSFCPHCGQEIKKSSPDKTKEKEEND"
     gene            3670..4971
                     /gene="purB"
                     /locus_tag="PK016_00260"
     CDS             3670..4971
                     /gene="purB"
                     /locus_tag="PK016_00260"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006323587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HXL01545.1"
                     /translation="MIERYTLPEMKKIWDDAHRFEIWLKIELLALEAQQKLGLLSPGD
                     LEKIKKGVSFSPDRMQEIEKITHHDVIAFLSTLSENLGPESRFIHFGMTSSDVLDTAN
                     AFLMKEALDLVLKDIDGVLNSIKNKALQYRYTLIVGRTHGVHAEPTTFGLKMAVWYAE
                     MLRNKERLEKARENIRVGKISGAVGNFAHLDPGVEEYVCKELGLEPDPVSTQIVQRDR
                     YAEVIWALAMVGGSLEKFAVELRSLQRTEIREVEEGFSKGQKGSSAMPHKKNPITGEQ
                     ICGLSRVLRGNLMAALENIPLWHERDISHSSVERIILPDCFILTDYLLHTFQRLLDNL
                     VVYPENMKKNLEKSRGLVFSERILLELIKKGLTREEAYALVQRCAMRTWEDESLSFLE
                     VLKNDSSIREYFSPEELESFFDYNYFLRQVDFILKKVGIEG"
     gene            4968..5999
                     /gene="purM"
                     /locus_tag="PK016_00265"
     CDS             4968..5999
                     /gene="purM"
                     /locus_tag="PK016_00265"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HXL01546.1"
                     /translation="MNIEERWSYKKAGVDIDLGDKLIQDIKGLVSRTYRPEVISGVGG
                     FSGVFQLGEKWNEPCLVAGSDGVGTKLKIAIDLDKHDTVGIDLVAMCVNDVLCVGAEP
                     IFFLDYLACGRLEEGKFKSILSGIVRGCEEAGCTLLGGETAEMPDFYKEGDYDLAGFA
                     VGVAEKKALIDGGKIQKGDILLGLASSGLHSNGYSLVRKVLQSQGMSLSSQIEGKILG
                     EELLRPTRIYVKSVLPLFREVEVKGVSHITGGGMVGNIPRVLPPHLGVKIYKSKIEIP
                     GIFSYIQKLGNIEEEEMWRVFNLGIGLVLIVSREEADKASRILEKEGEKVTVIGEVIE
                     SDRKVLVVE"
     gene            5992..6606
                     /gene="purN"
                     /locus_tag="PK016_00270"
     CDS             5992..6606
                     /gene="purN"
                     /locus_tag="PK016_00270"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HXL01547.1"
                     /translation="MSDKKIVVLVSGRGTNLQALIEATQSGYIPGKISLVISDNPQAL
                     ALQRARKASIPTLILDYNTFPHKRAYEDALLQVLEKESPYLICLAGYMRIVGKKIIAQ
                     FQQRIMNIHPSLLPAFPGLEAQKQALEYGVKVSGCTVHLVDEGMDTGPIILQASCPVE
                     DEDTVESLSERILQYEHQIYPQAVKLFLEDKLNVVGRRVFVRKG"
     gene            6612..7892
                     /gene="purD"
                     /locus_tag="PK016_00275"
     CDS             6612..7892
                     /gene="purD"
                     /locus_tag="PK016_00275"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008286810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HXL01548.1"
                     /translation="MKVMVVGSGGREHCLAWKISHSKLVDKIYCAPGNGGMSEIGECV
                     PWKSLSDLVSFAQEEKINLTLVGPEAPLSQGIVDLFEAHSLPIIGPDQRGALLESSKI
                     FAKNFMKKYQIPTSSFETFDDYEEAVDYLKKRDAYPLVIKADGLAGGKGVYVAYDVDE
                     ALDALTELMVDLKFGGAGERIVIEDFLEGEEATLMVLWDGKSYFLLPSSQDHKRVGEG
                     DIGPNTGGMGAYSPAPVVDEIVREKIEKEIVHPLLEGIEKEKLRYRGVIYLGVMVDKE
                     KEPRVLEFNVRLGDPEAQVVLPRVNNDWLEVNLAIWEGKLDKIKFSVNDSPMLGVVLA
                     SKGYPGNYEVGKKISGLEKFPNRVDDEVLVFHAGTEKRADGFYTAGGRVLNVCARGKT
                     LQEAKDKAYRAITEIHFENIYYRRDIGFRAIGEK"
     gene            7894..8382
                     /gene="purE"
                     /locus_tag="PK016_00280"
     CDS             7894..8382
                     /gene="purE"
                     /locus_tag="PK016_00280"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012082884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HXL01549.1"
                     /translation="MALVYVICGSKSDLEYGKECTRILDKLGIESQIFIASAHRSPER
                     VDEIVEKGEREGVGVIIALAGFAAHLPGVIASKTIIPVIGVPINSSPLLGIDALLSIV
                     QMPGGVPCAGVGIGKSGAQNGAILAGEILALQDSKIKQKLQELRLEQKKAVEKANEEL
                     LK"
     gene            complement(8537..10516)
                     /locus_tag="PK016_00285"
     CDS             complement(8537..10516)
                     /locus_tag="PK016_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL01550.1"
                     /translation="MSVNNNENNNLKAQPPRRLGGFGGGRAAAMPAEKAKDFKGTLKR
                     LIAYLKPQAMPLLLVIIFTALSVTFTISAPKIIGEATNELFEGIISKQLPAGTTKDQA
                     ITELKAQGQDQLAEMISGMDVTPGKGVDFNALLHILIILLVVYLLAAFFQWLQQYTMA
                     GVAQNTVYRLRRDVDGKLARLPLKYYDNHSHGDILSRVTNDVDNIAHTLQQSITQVVH
                     AIAVVVGVLIMMFSISPLLSWISLIIIPLSLLITMFVIRRSQKYFIAQWDSTGTLNGH
                     IEESFTGHNVIEVFNQQDKVKERFDRENERVYEASFKAQFISSIIRPAVNFLNNVNYV
                     VICIVGGIRVATGLITLGGVQAFIQYSRQFTQPVVQVASIINILQSTVASAERVFELL
                     DEEEEIPDTDNPVVLEEVKGHVKFEHVYFSYSPDTPLIEDMNLDAHPGQTVAIVGPTG
                     AGKTTLVNLLMRFYEIDSGRITIDGVDIRDMRRDDLRKIFGMVLQDTWLFNGTIRNNI
                     AYGKEGAAEEEIIAAAQAAYVDHFVRTLPQGYDTVLTEDASNISQGQKQLLTIARAFL
                     ADPKILILDEATSSVDTRTEMLIQKAMAKLRKGRTSFVIAHRLSTIRDADTILVMNQG
                     RIVEQGNHEELIAARGFYYDLYMSQFLSPVSAEVV"
     gene            complement(10513..12246)
                     /locus_tag="PK016_00290"
     CDS             complement(10513..12246)
                     /locus_tag="PK016_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL01551.1"
                     /translation="MKDLLVKFLKPYWKQVTLTVVLLLLQVMSNLYLPNLNADIINNG
                     VAKGDIRYILKTGSFMLVVTLLLAACAIASSYFSSKISMSFGRDMRRAIFHKVESFSQ
                     ADLDKFGTPSLITRNTNDVQQMQMLVLLGLNLMVMAPLMCIGGVIMALRQDVVLSSSI
                     IVIIPVMAVAVWIILSKATPLFRSIQIKIDRVNQIMREKLMGVRVIRAFVRNDYEAKR
                     FDETNRDLTTTTLRVNRIIALAIPSLIIIMNMSTVAIIWFGGYRIDSGAMPIGNLTAF
                     LTYIMEIMISIMLAMSMFVMVPRVEASAERVQEVLNTKPSIKDPTTPVEPRKSIGTVE
                     FYNVEFSYPGAEESVLRDISFIAVPGKTTAIIGSTGCGKSTLVNLIPRFYDITGGSIK
                     IDGVDIRDMHLRDLRDRIGLVPQKAFLFSGTIADNLRYGNENATDEELWHALEVAQAK
                     DFVMEIPGQLYAPVEQGGVNFSGGQRQRLAIARALVKKPEIYVFDDSFSALDFKTDAQ
                     LRLALKKETVHSTVIIVAQRVSTIMDADQIITLNDDGTIAGIGTHAQLMETCEVYRDI
                     VYSQLSEEEVA"
     gene            complement(12269..12748)
                     /locus_tag="PK016_00295"
     CDS             complement(12269..12748)
                     /locus_tag="PK016_00295"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXL01552.1"
                     /translation="MLNKESSKVNFLNLEGIDPISLQAFQSLSKFMRFHSQLLFKLTS
                     EKGIYPGQAVCLWFINQNSGISQRDLAEKMHVAPPTVTLMLQKLEKAGLVIRKEDERD
                     QRLSHTYLTNAGVDTLNVLNSILAEIINTSLENLSHDEQRNLMRLLDIINSNIERKL"
     gene            complement(13095..13730)
                     /locus_tag="PK016_00300"
     CDS             complement(13095..13730)
                     /locus_tag="PK016_00300"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HXL01553.1"
                     /translation="MEYLKILFKLLLAMALGGIIGWEREIVNKPAGLRTYVLTCLGAT
                     LITIISVTLPGSDPGRIAAQIVSSMGVLGAIVVLREGTALRGITTAIAMWLMAGVGIA
                     IGVGEYFAAIVTTLLAYGVLVFLRSFEAKILKHGTGRLLTVKAKNRPGLIGDIGSALG
                     NYEVNIVDIKLEISGEEVEISFKVTAPPHVDLSSLLQKVQSFPDIQTVRWE"
     gene            complement(13763..15091)
                     /locus_tag="PK016_00305"
     CDS             complement(13763..15091)
                     /locus_tag="PK016_00305"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-1-phosphate dehydrogenase"
                     /protein_id="HXL01554.1"
                     /translation="MSEKLFFFSEATSFPCKCGKIHHIPTKKTILGKEVLPRIPQTLS
                     ELNLGKKLLLVFDSNTYEAAGRETERILKKASFEVIPILLEKPPELKYLEPDEKARER
                     IGSFLFHEPDFILAVGSGVINDLAKFVAHRVQIPYAVVATAPSMDGYSSSGASMLVGG
                     YKTTYDAAPPCAIFMDLEVLSQAPLSLIQSGFSDLVGKIIANADWAMRNTLQGEYFCE
                     YTWELVKGTLEILKEKAEEIGERKEEAIYALAVALLNSGFTIVMVGDSRPASGAEHLV
                     AHYLEMMALHRKTDPSLHGLRVGGATVIIKKMYDRFSQELEEIDWKKSKNCSSKNQEE
                     ELKLHFGPLFPFLEKDVQEKLKWDREEIKIVDPSFLSQQLQDKLSVIPDPASLLRRAQ
                     AHQTLKELGFSEKTIREALLFSRFVRKRVTILDLLGKAGLLEEYIDYALS"
     gene            complement(15103..15885)
                     /locus_tag="PK016_00310"
     CDS             complement(15103..15885)
                     /locus_tag="PK016_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004100195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HXL01555.1"
                     /translation="MKFSAIRFLILDMDGTFYLGEHLLPGAREFLQTISEQGKDFCFL
                     TNNSSHSSAFYAQKLRKMGLQDFPSHKIITSSQATIFYLSQMNPHSRVYLLGTQELAI
                     EFLQANFILVQKDPDFVVLGFDTDLTYQKINRACQFIRKGIPFFATHPDLNCPTEEGP
                     VIDAGSFIKAIEASTGQSPKVFGKPYPEMIEYILQKTGTKKEEVAIIGDRLYTDIAMG
                     KKAGITTILVLTGETKEEDIKNSPWQPDFIFTSLEEINNYLK"
     gene            16052..17179
                     /locus_tag="PK016_00315"
     CDS             16052..17179
                     /locus_tag="PK016_00315"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HXL01556.1"
                     /translation="MIRIGVDKLKSGMIVGYPIMREDGVVFVKDGVVLNDTLINRLKK
                     LGFESIYIRDSRFPDIDLEEVISHDLRQQALMTLNETFYQVIKGKEVSIEPVKELVDG
                     IIEEILSSKKSVVSLVQLRQHDDSVFAHSVNVAALSAFVGKFLGFSHSQLKMLALGSV
                     MHDLGKIKVPVEILNKPDKLSPKEWELIKKHSSWSAELITEKMPEERESINIALQHHE
                     RMDGSGYPYGLKGEDIHFLSRICACADVYDALTVDRPYRKRFSYAEALEYLMGNSHSL
                     FDLQVVTAMVRHIAPYPVGEVVKLTSGETAVVVKLNEGLPIRPVVRVIKDKEGNIVGK
                     PRDIDLMKELTVAILCSGDETPEDFYSSSDVSCFKEMGFID"
     gene            complement(17176..18027)
                     /locus_tag="PK016_00320"
     CDS             complement(17176..18027)
                     /locus_tag="PK016_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXL01557.1"
                     /translation="MRIIKPFDPWQGKWCSCPPKYTLNPYTGCAHHCLYCYTTSFIPR
                     AFEVREKPGILKKVEKDLEEIDPSRYLSLSNSSDPYPPEEEKRKLTREILKLCQKKGV
                     PILILTKSTLVIRDIDILKTMKAAVSITITTFNSHITSLLEEGAPSPQERARALIELH
                     QAGIPTILRIDPLIPEINDKTEDWKEILSLLSPYINQVMVSTFKPRPDSWKRITTAFP
                     SLQKTENWYTKKRGNSLYLEDNRRIKLLEEARTIIHSFSLPFSSCREGMGIWNDLPCD
                     GSNFIRN"
     gene            18750..19472
                     /locus_tag="PK016_00325"
     CDS             18750..19472
                     /locus_tag="PK016_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HXL01558.1"
                     /translation="MQGINMEERKKILDMYKDLRVTDVRDGMDWCGYHHYGSVHHSIK
                     PLWRPCKAIGFARTARYLPYEGPSPKETGEKYTEWSRWYYQNVCTDDWGKDIIEGDFI
                     CLDVCGINVGLLGSNNTLAYKNKGAVGFLVNGGGIRDTDEVILEKVPVWSKFISQPMD
                     QARIRYYEKDIPIAIGGVAIYPGDLVVADGDGVIVVPAKMIYEVHKYAYQEMKNDKIA
                     RRKLYIEGNLPLDDTVDTEGLK"
     gene            19564..20865
                     /locus_tag="PK016_00330"
     CDS             19564..20865
                     /locus_tag="PK016_00330"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXL01559.1"
                     /translation="MSKKVLLFLVVAMLSAIMVFSTGLAFAEQVEIRAAWWGDTGRHE
                     LYNKIIDIFESKNPDIKVIREPTSWLDYWDKLTVQSAGGRAPDFIGMHLQFAADYVRR
                     GILEPLEPFVGEGVIDISKHSEAAISTGTMDGVLYMIPMGLTTQTMVVNNSILEELGV
                     EPPNFDWTWDDVKTIGLKVRGAFDEKGDKNKWFIADSSGAYQIFRYWVRQRGQEDLYT
                     ADGNIAYTAEDAATWFTMWNDLRENNIVPDAATTTEYARATLEDNLFARQRIIITGIP
                     VNQYKLYLNAVPGANLLLVRNPSLVDGLPGEFVEGAHFAVSAHSTPEKKLAAAKLIDF
                     WVNDPDSIELYRLDQGVPSNREMAEFLMTIVDEADQKIIEYVNKTSEIARPINFPPAG
                     ASEVNSLFEQIAEKVRFGQLTPEDGGEQLVSQAQDILNKHK"
     gene            20934..21812
                     /locus_tag="PK016_00335"
     CDS             20934..21812
                     /locus_tag="PK016_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXL01560.1"
                     /translation="MRKYIYNREGYAFLTPWLLGFILFTAIPMILSFYFSFTQYDVLT
                     PPKWVGLRNYIDMANDPRVHKSLQVTFTYVGLGVPLQIIFALFVAILLNRDRPGIRTY
                     RAIYYLPSLFGGSVAVAILWRQVFNKEGLINQFLALLGIEGKNWIASPETALYTLIVL
                     LIWQFGSPMVIFLSGLKQISKEYYEAAMIDGAGRIRMLFSITLPLLTPLIFFNLVMGI
                     INAFQAFTPAYIISGGTGAPVDSTLFYTLYLYIKGFQHFSMGYASALAWVLLVIISIM
                     TALMFLLSRFWVHYEE"
     gene            21825..22664
                     /locus_tag="PK016_00340"
     CDS             21825..22664
                     /locus_tag="PK016_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXL01561.1"
                     /translation="MGEQNRSIVIYYLFITLFALVMIYPLLWMTSASFKEDVEIFQSP
                     SLIPQNPTFNNYAYGWRGVSGYTFGRFLFNSFLISFIGIIANILSCSMTAFAFAKLDF
                     RFRNIFFAIMMITLMLPFHVRLIPQYIIFNKMGWVDTFLPLTVPRFFATDGFFIFLLV
                     QFMRNIPNEIMDAARIDGASAWQIYARIMMPLSTPALVTVAIFTFIWTWNDFFSQMIY
                     ISSPSKFTVSLALRMFVDATGESSWGALFAMSCVSLLPLFIIFIVFQRFLVEGIMMGS
                     LKG"
     gene            22691..23095
                     /locus_tag="PK016_00345"
     CDS             22691..23095
                     /locus_tag="PK016_00345"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXL01562.1"
                     /translation="MFQGLYHIGIYTTDIERSLSFYTQILDFNLKWRGMVDHPAMGKI
                     KVALVEQQNCTIELVQPNDAALVNEKAGPIQHLALKVESIDKVMERWKSKKITEFPLG
                     EIEDLPSFGKGIRHTFIYGPSHERIELVEEYT"
     gene            23128..23562
                     /locus_tag="PK016_00350"
     CDS             23128..23562
                     /locus_tag="PK016_00350"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HXL01563.1"
                     /translation="MEYFVTGKAGNRVFVLRLDPGDMVLESINELIAKEEIKDAAVIS
                     AIGTLDRCVLHMVTTTDYPPKEHFERWEDTPLELASIDGIIADGKPHLHAVVSDTEKA
                     YAGHLENECRTLYLGEIVIMELGSMDLTRVYNEKKVLKLVKK"
     gene            23622..24329
                     /locus_tag="PK016_00355"
     CDS             23622..24329
                     /locus_tag="PK016_00355"
                     /inference="COORDINATES: protein motif:HMM:NF015682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01564.1"
                     /translation="MTDEELIKEIKKHRLADLSDGLDALGLVNVGTMDPQMRPIREGI
                     SFAGFAYTVKLVPAQKSVKACNSIEEYLKELDSWCSDAYAFMSGITEETAKNMVVVID
                     EGGYPGGVWGSEIAMNTMKKGVVGAVIDGACRDSYECNLEKVNVFCTKRTFNHVYGRL
                     NNGGVNIPIRCAGVAVNPGDVICADDDGVLVIPRDKAEKVLEFAAVIHKNDQETRARH
                     YRDLGYEFDSTLGDIEG"
     gene            24542..25843
                     /locus_tag="PK016_00360"
     CDS             24542..25843
                     /locus_tag="PK016_00360"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXL01565.1"
                     /translation="MSKKVLLFLVVAMLSAIMVFSTGLAFAEQVEIRAAWWGDTGRHA
                     LYNQICDVFESKYPDIKVIREPSSWTDYWAKLNVQSAGGRAPDFIGMHPFFASDYVQR
                     DVVEPLDDYIKNNVIDVSKFSQSAILSGTVNSIVYMIPMGITIDTLFVNNSFLKELGV
                     ATPEFDWTWDDFKTIGLEVRKALDAKGEKDKWFMDDISGIYEIFRVWVRQRGREDLYT
                     PDGSIAYTAEDAASWFAMWNDFRENGIIPDAATTTEYMNATLEDSLFGRRRAAVRRMP
                     VNQYKLHCAALPDDEIIMVRHPSKPDGEIGEFIYGAFFAISASSAPEKKLAAAKLINF
                     WVNDPDSIELFRLDQGVPANTEMVDFLMTIVDEKEKAILNYVNELMPLARPIIFAPAG
                     ATEIDSLFKQIAEKVRFGQLTPEEAGEQLISQAQDILNKHK"
     gene            26446..28557
                     /locus_tag="PK016_00365"
     CDS             26446..28557
                     /locus_tag="PK016_00365"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HXL01566.1"
                     /translation="MKEYPFGAAFFMPSEGIANDLVNMKAAGMNVARLEWIKNAVWEA
                     IEQSPDTYSFGVIDEVMEAAANTSLKVMLQIGIYPPRWVRDTYGGTGVVNERGLTPHP
                     REKFSICYDNPQIIQLAEKYIRTMVRRYKNHPALYGWISWNEPHLSNEDITCYCPHTM
                     KAFRIWLQKKYGNLKTLNEAWIEGYPAYTSWDEVEPPRQMPRERGIYQAWQDWRNFMD
                     ENFSNLLKWVTSVIKEEDGYHPTKVNLLMPTLNTTIICSDVWKMADTADEIGVSLFVD
                     TNEGEYPQLFSQSADLIRSAIAYHDRPFWLDETQGGPNFVSHRYPVLPQDKKISLYPW
                     QSVAHGAKGFTYWMWRPLTIGHEAGEFGLVGKDGSPRPRTAEAARAGAIIQKHTDIIL
                     NSEPDNKVCIMHSQEIFHLTYGEGLDTHIKSFPLDNPPQKSRYASALLGAYTILWEEK
                     FGVDFISPEHIENGGLEKYRALVMPFPYLMSEDTAIAVKNFVATGGFLISEFPNIMKD
                     LGGKIYKITPGAGLRDMFGFEEIDFGPTDGRPIVLSERAKVATGQMKQIVQVDSHCNI
                     IGRFPDGSPAVIFNRYGQGGVLSFCMEVFQPVMWNNHDALASLIEKFLQIYGITKAGE
                     LHGIEESLARRVEIAELTNPNGKLVIVINHNHQAISGSLYLNQGSKYINLENGEELIP
                     DRGKLFLNLEGFGVLALFCTA"
     gene            28960..29823
                     /locus_tag="PK016_00370"
     CDS             28960..29823
                     /locus_tag="PK016_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HXL01567.1"
                     /translation="MCVKHWIDAMVENISFHEEELKDFASRVRKRIIKATYKAGVSHI
                     GGSLSVVEVLTCLYNGVLNINQENLLSRKRDQFILSKGHSALALYSVLAEKGFMEETL
                     FEEYGVREETILGIHPELDVPGIDAATGSLGHGLAIGIGQALAFKMQGLDYKVWVLTG
                     DGEFNEGTNWECLPLALRYQLDNLVLIVDRNRLQLSGFTEELHPLDPLDEKLRAFGWS
                     VEMVDGHDFPMLWKLLVRIKEYRGCKPTVIIAKTVKGRGVSELENKVDSHYCGVPPEV
                     AEKYLGVLPDE"
     gene            29816..30748
                     /locus_tag="PK016_00375"
     CDS             29816..30748
                     /locus_tag="PK016_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HXL01568.1"
                     /translation="MNRRDAFQEVMLELMTAGKKMVCISVDSASRFKEVKARYPERFV
                     ECGICEQVGMSVCAGFALQGMIPVISGYATFLTMRAFEQIRTDICKWSLNVNICGTDT
                     GFSPQYSGFTHQALEDIALLSSLDNITVAEACDYQDALLMSKYLFGEVEGPVYLRFGA
                     RGKEWVFGECHQPVRAGYFEPLSKENFDGADVTIIALGQLVEVGLEVAEVLGGKGIKT
                     FVFNARFVKPLAQKMIEEIASGSKLVVSIEEHSVTGGLGDNLLRAFHEVGRVPRLLKF
                     GVPSNFGISGKRDYLLRKCGLSVDSIANRVLSVF"
     gene            31495..32460
                     /locus_tag="PK016_00380"
     CDS             31495..32460
                     /locus_tag="PK016_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HXL01569.1"
                     /translation="MEEKVEIQNQEIDMMVKAAELYYVSGLTQGEIAKILGVSRQKTF
                     RLIKKAREMGLVEVKIRRPSETHEKLSFELQKKFSLRGALVAKAYSSTLDSIIKAIAQ
                     SGAGYFREQLQPYNTIGVAYGRTLHEVIQYFPKIDVPGIKVVQMMGAYGGIRWQTLAI
                     ELIKTLADKLGGEAVYLFAPAFAESSDIRDAFLRERSIQETLKMAESVDLALVGIGGI
                     QREASLLIETGDLRKEEIEELARAGAVGGICGNFFNEEGAIIIANLNSRRIAVRLEEL
                     RKNSKKVIGIAGGEEKKRAILGALKGHWITHLVTDENTARWLLEY"
     gene            32520..33041
                     /locus_tag="PK016_00385"
     CDS             32520..33041
                     /locus_tag="PK016_00385"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HXL01570.1"
                     /translation="MSFQKEVNWLEAGKIALETLENLGGVFLVSQNKENLRPNVMTIG
                     WLELGIVWGRPVITVLVRPSRYTFSLLEKNPFFVMSVPPLSWKKEIDFCGSTSGREVD
                     KFSRWGFNPVNIGNFPVPAIGECEVALGCEIIQKTRVEAGSFSASILNRFYPQDDYHF
                     IYFGEIKESWSKG"
     gene            complement(33044..33787)
                     /locus_tag="PK016_00390"
     CDS             complement(33044..33787)
                     /locus_tag="PK016_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HXL01571.1"
                     /translation="MSLVIWVIVIIAVIILASAIRIVREYERGVIFRLGRLIGAKGPG
                     LFFVIPFMDQLIKIDLRVLTMDVPKQSLITRDNVPVTVDAVVYFRVLNPEDAVTKVEN
                     YISATSLISQTTLRSVVGQSELDELLIHRDKINEALQKIIDEKTDPWGIKVSAVEIKE
                     VTLPEELKRAMARQAETERERRAKIISAEGEFQAAEKLSQAAEIIARNPIALQLRYLQ
                     TLNDISAENATTIVFPLPMEFLKAFGTKD"
     gene            complement(33790..35031)
                     /locus_tag="PK016_00395"
     CDS             complement(33790..35031)
                     /locus_tag="PK016_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HXL01572.1"
                     /translation="MKRKNLKIILLLLLVVAILSWNSFVAAQEKVVIGKLEGVINPVT
                     EQFVVRLIEEGEKEEAQAILILLDTPGGLEESMRNIVKAILQSPLPVITYVYPPGSRA
                     ASAGSFIVMASHHAFMSPNTTIGAAHPVTIEGQEVGEKIVNDSASFIKTLAETRGRNP
                     EIAEKMVRESLSLTEKEAQEKGVIDGIAESPEEVIEKLGIKNPHILEVEMKGKEKFLH
                     FLLDPTLAYLFLVIGALGIIFELSNPGAIIPGVFGTILILLAFYALSVFPINLTGILF
                     IFLGLLLFILDILVTPGIGILTAGGAVALFFGSLMLFDVQGGITLPLNIIITTVICVT
                     VFFAFALGMALRAMKKKVVTGKESLIGQTGIAKEDLNPEGFIIVEGELWWSKAENPVK
                     KGEKVVVSQKEGQTLIVKKEE"
     gene            35101..36435
                     /locus_tag="PK016_00400"
     CDS             35101..36435
                     /locus_tag="PK016_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXL01573.1"
                     /translation="MNILLVAPGTDNLQRKMRIVPFPQASLPLLAALTPSQHEVKIID
                     ERLENIDFDEKWDVVGITVMTATSHRAYYIADEFRKRGIKVVLGGIHPTALPDEAIQH
                     ADSIVIGEAEGLWEELIKDLEKGELKKFYSHDCPPPLTGLPFSRLDLLHKRYLLKHVF
                     QTTRGCPHNCGFCSVSTFMGRRYRHRPAEEVIKEISLYPNSRIIGFLDDNIVGNPRYS
                     KELFQALIPFQKKWVSQGTLKLAENEELLKMAAKSGCIALFVGFESINEDNLRKMHKN
                     FNQVDKYKSLIDKFHEYGIMIIGSFVFGFDEDDPSIFQRTLNFLEGVNLDFAQFSILT
                     PLPGTEVFKQFSEENRIFSYDWRKYDFAHVVYRPLRMTPQELQDGYNYIFRQFYPLPR
                     VIKRTMRNWRYAHYFLPVSLYYHWVSLHPKSLPEEVASALFSLKDKKHSPVI"
     gene            37256..37894
                     /locus_tag="PK016_00405"
     CDS             37256..37894
                     /locus_tag="PK016_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXL01574.1"
                     /translation="MKIAVVCASGRVGRLLVEEAILRGHEVTAIVRNPSKAPQATKVI
                     VKDLFDLTLPDVKDCEVVIDAFGTWTPDTLFQHSTSLKHLCDILSGKPNRLLVVGGAG
                     SLYVNPEHTIRLMDTEDFPDAFKPVASSMAKALDELRNRNDVRWTYVSPAADFRVDGP
                     RTGKYILGGEELILNSKGESVISYADYAIAMIDEAEKDNHIDQRISVVGEYD"
     gene            complement(37903..38343)
                     /gene="mscL"
                     /locus_tag="PK016_00410"
     CDS             complement(37903..38343)
                     /gene="mscL"
                     /locus_tag="PK016_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HXL01575.1"
                     /translation="MMKEFKEFASRGNIIDLAVGIAVGGAFGVLVQSLVSDLIMPPIG
                     LLLGEIDFSNLFVVLKPGNPPGPYLTLTSAKDAGAVTINYGLFISHLVSFLIIAFTLF
                     LVVRTINQFQRKEEAPSPATKECPYCHTTIPIQAVRCPNCTSQL"
     gene            38629..38708
                     /locus_tag="PK016_00415"
     tRNA            38629..38708
                     /locus_tag="PK016_00415"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38666..38668,aa:Met,seq:cat)
     gene            complement(38757..39080)
                     /locus_tag="PK016_00420"
     CDS             complement(38757..39080)
                     /locus_tag="PK016_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HXL01576.1"
                     /translation="MTTPEEFKEDVLILAKEVGVNPREIHIQTMKRKWASCSSRGRLT
                     FDKSLLNEPKEVRFKVILHELLHLKYPNHGKMFNSLLDTYLQRNINSNEEKEAKDHQL
                     PDESN"
     gene            complement(39077..42028)
                     /locus_tag="PK016_00425"
     CDS             complement(39077..42028)
                     /locus_tag="PK016_00425"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HXL01577.1"
                     /translation="MSSLGGEKYAVQKPIINYVKEQSAEYISQDGNKIFIKLGWEYVS
                     PDEALRLRGGKTGLVFKEIFINQMQRLNPSFMDHLLAEEVIKKLETVLPNIEGNFTAW
                     KYLKGLGTIFVPGEKRERNIKFLDTEDVDRNTFHVTDEFEFTNGTKTIRPDIVFLVNG
                     VPLLLIETKAAYKIEGISEALEQVKRYHRECPELLAILQVYALTHILKYYYSATWNTS
                     EKLLFNWKEEVSGDFESLVKTFLDRERVVKLLVDFLMFTRQDDELKKVILRPHQMRAV
                     SKIVERAEDSTKKRGLIWHTQGSGKTYTMIVAAQKIIENPVFENPTVIMLVDRNELES
                     QLFGNLAALGIENVEVAESKKHLQDLLSRDRRGLIVTMIHKFEDMVENINLRKNIFVL
                     VDEAHRTTGGKLGNFLMGAIPNATYIGFTGTPIDKTSYGKGTFITFGRDDPPKGYLDK
                     YSIAESIEDGTTVPLYYTLAPNELQVDKEVLEREFLDLAEAEGMSDIEELNKVLDKAV
                     TLKNMLKNRERVEKVTRYIIDHYQKYIEPMGYKAFVVAVDREACALYKEELDKYLPPD
                     YSKVVYSPFYNDPPELSRYHLSEMEEKRIRNAFRKPDELPKILIVTEKLLTGFDAPIL
                     YCMYLDKPMRDHILLQAIARVNRPYEDDEGRKKSSGFVLDFVGIFDNLEKALAFDSQD
                     IEGIVQDVEILKKRFAEEMKKAREKYLTLIEGKAQDKAFEAVLEYFRDEEKRKEFYKF
                     FKELSAIYEIISPDAFLRPYVNDYETLSRMYKSLIENYERGIPIDREFTRKTAMLVQE
                     HTRSGVIGPTLDICEIDEQTLRRIEKSKASDTEKIFNLIKSIALTVEKRGGQSPYLIS
                     IAERAELIAKLYESRQKTTQETLEELKKIIDEINSAQKEQIEKGMSADIFTIYWMLKE
                     KGFQDAEAMANQMREVLEKYPHWQKSERYEREVRSKLYEVILHLYPTDIPKVSEVVQN
                     IMDILKAGSTE"
     gene            complement(42025..43338)
                     /locus_tag="PK016_00430"
     CDS             complement(42025..43338)
                     /locus_tag="PK016_00430"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HXL01578.1"
                     /translation="MNELDRDLPEGFKITELGPLPEEWEVVRLGEVAEISSGGSAPQG
                     DEYFKGEKPFIRVSHIDNEDCVIKEYDLINDKAVQDYKLKLFPKGTIVFPKSGATIYL
                     EKRAMLPTDAYVVSHLCTIIADEKNATQDFLFYLLRELKLAKEKADGYPTLNISEIKQ
                     IIFPLPPLPEQRKIAAVLSAVQEAKEKTEAVISAAQGLKKSMMNYLFTYGPVPVVDAE
                     NVPLKETEIGMIPEEWEVLRLGDYDLLKKAQYGLSIRGSKLGKYPILRMNNLINGAIS
                     TDDLQFVNLDESTFRRFKLEKGDILFNRTNSFELVGKTSVFTLNDDFVFASYLIRLRV
                     NDSKIFPLLFNFYFNWDITQGRLKCLATRGASQSNISATKLKTFLIPLPPLPTQQKIA
                     SMLSAIDEKIEAEENKKKALEDLFKTLLYNLMTAKIRVNNLEVGV"
     gene            complement(43338..44873)
                     /locus_tag="PK016_00435"
     CDS             complement(43338..44873)
                     /locus_tag="PK016_00435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HXL01579.1"
                     /translation="MPGGELDVKTLEGWLWEAACKIRGPIDAPKYKDYILPLVFLKRL
                     SDVFDDEINRLSQKYCSREIVEQLLAQDHNLVRFYLPEKTRWEEIAKQTTNVGEYLTD
                     AVRTIARENDKLQGVIDAVDFNATAAGQRIISDDRLKELIDVLGRYRLGLSDVDADIL
                     GRAYEYLLRKFAEGSGQSAGEFYTPGEVAILMSYILNPEPGDEIYDPCCGSGGLLIKS
                     HLRFKEKYGADSSLEPLKFYGQEILHSTFAIAKMNTFIHDMEAHIELGDTMTRPAFLT
                     PEGSLRKFDLVTANPMWNQNFPPSTYENDPYNRFIFGYPPSSSADWGWIQHMFASLNE
                     KGKMAVVIDTGAVSRGSGNEGRNRERDIRKEFVEGDFVEAVLLLPENLFYNTTAPGVI
                     IAINKSKPAERKGQILLINASKLFKKGRPKNYLPDESIKQISDIYLQWREEEGISKTI
                     TREEAARNDYNLSPSRYVAQNGEDDTLPLEDAVVQLKEAEEERRKADAQLKQVLRELG
                     VEI"
     gene            44970..45046
                     /locus_tag="PK016_00440"
     tRNA            44970..45046
                     /locus_tag="PK016_00440"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45004..45006,aa:Met,seq:cat)
     gene            complement(46198..46926)
                     /locus_tag="PK016_00445"
     CDS             complement(46198..46926)
                     /locus_tag="PK016_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HXL01580.1"
                     /translation="MTEYVERWYRKCTRAPDLVPFSVQIKESDLFILAQKNLEDLAYK
                     RLEKERNNLEAYLRYDPDFLHSLVPVTAPPFAPPVCQMMAEAAKKAGVGPMASVAGAI
                     NEMVGEELRQESQEVIIENGGDLLVFSQEARVVAVYAGEDSPFSFRIGIKLSPGKKWG
                     VATSSGTVGYSLSFGKADAVVVVAESSAVADAWATSLANRVREKEDIKKVLEFSRQIT
                     DIQGILIILGKFLGVEGNIQIEKL"
     gene            complement(46923..47333)
                     /locus_tag="PK016_00450"
     CDS             complement(46923..47333)
                     /locus_tag="PK016_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXL01581.1"
                     /translation="MNSSQKVVLRFPGELVDRPLVCELALQFGLHFNIMRASISPHRE
                     GLMVIEIIGEKKKQEEGLRFLKENGLEVESLSQDIRMVEERCIHCGSCVGVCPTQALY
                     VEKPSYLVKYDETQCIACEMCVLACPTHAMEALL"
     gene            complement(47338..48561)
                     /locus_tag="PK016_00455"
     CDS             complement(47338..48561)
                     /locus_tag="PK016_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HXL01582.1"
                     /translation="MGEKLRTYEEINEKIRQGKAVVVTAEEVIDLVEEKGVEKVAQEV
                     DVVTTGTFGVMCSSGAFFNIGHTKPRMKVSEAYLNGVMAYSGLAAVDFYIGAAQVAQD
                     DPLNKIHPGEFKYGGGHVIQDLVAGKDVLLEAYSYGTDCYPRRKLVTWINLRDLNEAY
                     LFNPRNAYQNYNVAVNLSSKTLYTYMGVLLPKLGNANYSSAGQLSPLLNDPFYRVIGV
                     GTRIFLGGGVGYVAWQGTQHNPGVDRNERGIPKEGAGTLAVIGDLKGMNANWLWGVSM
                     RGYGCSMAVGLGVPIPILDEETLYYCAVKDEDILAPIIDYSNAYPNGTGEILGYASYA
                     QLRQGKIKIEGKEVPTASLSSYPRAREIASILKDWIKKGDFTLTQPVLSLPGKEGPQF
                     HNLPERPVNNGKTGR"
     misc_binding    complement(48609..48839)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT        14204 a         9215 c        10679 g        14993 t
ORIGIN      
        1 agcgtagtct tctcagagag aaaagccagc gtttcccatt gtggtctaag agagccctct
       61 ttcttcggtc ataaggtttt agttctccct ggcagtaagg acagtgaagg gtttacatta
      121 tatctggcaa tttagagaca gacaatctcg gtagcttttg gacgttctgg gttggtagaa
      181 ataggagaag gaaagaacat gtatgactct cccctcaagt aaaagagaaa tgagaaggag
      241 gtgaatatgg ttgttgacat tttatagggt agggtggtat actatattaa aacaaaaaat
      301 tagtgaggtt aaaaaaatgg aagaaataaa caaccatgaa ggcaatagtt tagaggatag
      361 cagacataga ctctgggatg atgaaaaggt agtgaaagaa attctggtga gactcaaccg
      421 gattgagggt caggtgcgag gaattaaacg catggttgaa gaagaagcgt attgtgatga
      481 cattttaaac cagattgctt ccattcagtc tgccctttat ggtgtagctc gcctgctttt
      541 agagaaacat atgaaatctt gcgtaaagga acaaatttta tctggggatg agagagttat
      601 tgatgaagtt ttagctacta tttttcggat gatgaaataa gaaatgtgga ggtcttaaac
      661 atggaagagg aaaaggagaa aaataatcag attcttacta ttcccatatc ggggatgaaa
      721 tgtgcagcct gtgcatctcg agtagaaaaa ggactagcag agattcccgg gattcattca
      781 gtaaatgtta acttagcttc aggtaaagcg acggtaggct acgacgtggg gaaagttgat
      841 tttcaggata tagtggataa agtgtcccaa attggttatg aggttccaga agaagaaata
      901 gaactcttga ttgaagggat gtcttgttct tcttgtgcag caaggattga gaaaaaactt
      961 aacacccttc cgggagttaa aagggcggcg gtaaacttag cttctaataa agcctatatt
     1021 cagtttgtgc ccggaataat taatcaggct gagatagaag aatctataga atctctggga
     1081 tatggtgttc aaactccttc tcgtgccctt tcagaaagag gaaaaggacg aaaacgaaac
     1141 agagaaaact ggcagaaaat tagattttta gcggctcttt ttttatcgtt tcctcttttt
     1201 tatatgatgg tagccaattg ggtggggaaa ggtcagttta tgattgaccc ctggattcag
     1261 ctccttcttg ctaccccggt gcaatttttc tccggttggc ctttttaccg gggagcttat
     1321 cattctttga agtcaggtag cgccagtatg gatgtccttg tagctttagg aacttcggta
     1381 gcttattttt atagtttaat ttctttagta gctggttggg gagtatttta ctttgaatcc
     1441 tctgctatgt tgattaccat aattttattg ggaaaactgc tggaagagag agctaaaggg
     1501 aaagcctctg aagctataga aaaactggta aagttgcaac ctgaggttgc tcatgttctt
     1561 caaaatgaaa tggaaaaaga tattccggca gaaagagtaa aagtgggaga tatggtgata
     1621 gtaagacctg gtgagcgcat ccccgtagat ggcgtaattg tagaggggaa ctctgccgta
     1681 gacgaatctt tgcttaccgg cgagagtatg ccagtagaaa aacatcctgg ttgcgaagtt
     1741 ataggaggct ccataaaccg ggaaggcagc tttattttcc gggctactaa agtagggtct
     1801 cgtatggtgt tagctcgcat tgttaaaatg gtagaagaag cacaaggctc caaagcaccc
     1861 cttgagcgtt tagctgaccg agtttctgct gtatttgttc ccgtaattat cgctctttca
     1921 gtcattactt ttttgggctg gtatatagaa ggggccggtt ttgctaatgc tcttatgcat
     1981 atggtcactg ttttagtggt agcttgtcct tgtgctttag gtttagctac tcctactgca
     2041 gttatggtgg gaattggtct gggagcagaa aaggggattt tcttccgggg aggagaatac
     2101 ttagaaggaa tagaaaaagt aaataccttt cttttagata agactggtac cgtcacctgg
     2161 ggaactccct cagttgatga tttttttatc ccttctcttt ttgatgtaaa tgaaacctgg
     2221 gcaataattg cttcaggaga aaaacggtct gagcaccctt taggtcaagc tctgaataag
     2281 tatggagaaa aacttggggt gcctaacagg gaaataaaga attttgaggc tttaccgggg
     2341 aaaggaattc gttttcaata ccaggatgag gtctggtata taggaacaga ggaacttttg
     2401 aaatctcaag gagtagattt aacggaggta gcagagtata aaaagcgatg ggagagagaa
     2461 ggtaaaactg tagttttagc tgcaacccgg gggaaggtaa gagcggttat tgctgtcgtt
     2521 gatactataa gagatgaagc tcgagaggtt gtgtccgagc taaaacgaat gaggttagag
     2581 gtagcgatgg taaccggtga ccacgaaata gtagctcaag ctatagccca gaaagctggt
     2641 attgataaag ttttttctga aattatgcct gatgaaaaag tggagatagt cagaaaatta
     2701 aaagaagaag gaaaagtagt ggctatggtg ggagatggca taaatgatgc tccagctctt
     2761 gcccaggctg atgtaggaat agctataggt acgggaactg acatcgctat agagagtgcg
     2821 gatgtaattt tgatgaatgg ggaactgaag agtctgattt ctgctttacg tctttctcag
     2881 tttacccgaa ggaaaataaa acaaaattta gtctgggcgt ttgtctacaa tctgattacc
     2941 gtacctttag ctatgtttgg tgtttttacc ccagttatgg gaggaacggc tatggctctt
     3001 agctccgttt ctgtggttac taattctctt ttgcttaagc gatatagaaa caagatttaa
     3061 gcgagttttt agacattccc gttggggctg gatatctgaa ataggtaata gccatcttga
     3121 aatgatgttt ttacaaaaac atcatttctt tatcctgtaa aatatgtaag atgaattatc
     3181 tctatgttaa aatgaaaaat aaatttagga ggactaattt ataaatgaat aaaaatagag
     3241 gagtaacatt caatttcttt cgttcctggc gtttacgctg gcagatcgct tccctagaga
     3301 aggaaagaga agaatctctt ctccggttag gaaagaaagt ttatgagctt tttgtagaac
     3361 ggaaaaactg gggaaaagaa gaagtaagag aaaattgcca ggaagtgctc tctattgagg
     3421 aagaaatcga agatttagaa gaaaatatac gtcgtttggg aggcaataaa ccccggcgag
     3481 tttgcccttc ttgtggagaa tacatagaag agagttctct ttattgccct cgctgtggtt
     3541 tttatcagaa gcaagaatta acttgttctc aatgcgggga gaaaataaac cctcaagaca
     3601 gtttttgccc tcactgtggc caggaaatta aaaaatcttc ccccgataaa acaaaggaga
     3661 aagaagaaaa tgattgaaag atacactctt ccagaaatga aaaaaatctg ggatgatgct
     3721 catcgattcg aaatctggct gaaaatcgag cttttagctt tagaagctca acaaaaatta
     3781 ggtcttctat ctccaggaga tttagagaaa atcaaaaagg gggtttcttt ttcccccgat
     3841 agaatgcaag aaatagaaaa aataacccat catgatgtca ttgcttttct aagtactctg
     3901 tcggaaaact taggacctga atctcgattt attcattttg gcatgacttc ttccgatgtt
     3961 ttagataccg ctaatgcttt tttgatgaag gaagctttgg acttagtgtt aaaagatata
     4021 gatggagtgc tgaattccat taagaacaaa gctttacaat accgctatac cttaatagtg
     4081 ggaagaactc acggcgttca tgctgagcct actacttttg gactgaagat ggcagtatgg
     4141 tatgcagaga tgcttcgtaa taaagaaagg ttagaaaaag ccagggaaaa tatcagggtg
     4201 gggaaaattt ccggagcagt gggaaatttt gcccatcttg accctggagt ggaagaatac
     4261 gtatgtaagg aattaggttt agaacccgac cctgtttcta ctcagattgt tcaaagagac
     4321 cgctatgccg aggtaatctg ggctttagct atggtagggg gaagtttaga gaaatttgct
     4381 gtagagctgc gtagtcttca gagaacagaa attagagaag tagaagaagg attcagtaaa
     4441 gggcaaaaag gttcatctgc tatgcctcac aagaaaaatc ccattactgg agagcaaatt
     4501 tgtggattga gccgggtttt acggggtaat ctgatggcag cactggaaaa tatccccctc
     4561 tggcatgaac gagatatctc tcattcttca gtagaaagaa taattcttcc cgattgcttc
     4621 attttaactg attatttgct tcatactttc cagcgcttat tggataattt ggtggtttac
     4681 ccggaaaata tgaagaaaaa tctggagaaa agtcgagggt tagttttttc agagagaata
     4741 cttttagaat tgataaaaaa ggggttaact cgggaagaag cttatgcttt agtgcaacgc
     4801 tgtgctatga gaacctggga agatgaaagc ttatcttttc tcgaggttct taaaaatgac
     4861 tcctccattc gggaatactt ttctcccgag gagctggaat cctttttcga ttataattat
     4921 ttccttcgcc aggtggattt tattttgaaa aaggtaggta tagagggatg aatatagagg
     4981 agagatggag ttataaaaaa gcaggggtgg atatagactt aggagataag ctaatccagg
     5041 atataaaagg tttagtctct cgcacctacc ggcctgaggt tatctctggg gtaggaggtt
     5101 tctcgggggt ttttcagtta ggagaaaaat ggaatgagcc ctgtttagtg gcgggaagcg
     5161 acggagtagg cactaaatta aaaatagcca tcgatttgga taaacacgat actgtgggta
     5221 ttgacctcgt agctatgtgc gttaatgatg tcttatgtgt gggggctgaa ccgatatttt
     5281 tcctcgatta tttagcttgc ggaagattag aagagggaaa gttcaaaagc atactatcgg
     5341 gaatcgtccg gggatgcgag gaggcaggtt gtactctttt gggaggagaa acggcagaga
     5401 tgcctgattt ttataaagaa ggcgactatg acttagctgg ctttgccgtg ggggtagcag
     5461 aaaaaaaggc tttaatcgat ggaggaaaaa ttcaaaaagg agatatcctt ttgggattgg
     5521 cttcttcagg cctgcacagt aatggttact ctctggtgag gaaggtttta caatctcaag
     5581 gaatgagcct ttccagtcag attgagggaa aaatactggg agaagaatta ctgcgtccca
     5641 ctagaattta tgtaaaatcg gttcttcctc tttttcggga agtagaggta aaaggagtgt
     5701 ctcacataac cggaggaggg atggtgggaa atattccccg ggttcttcct cctcatttgg
     5761 gagttaaaat ttataaatct aaaattgaaa ttcctgggat tttttcttat attcaaaaac
     5821 tgggtaacat tgaagaagaa gagatgtgga gagtatttaa tttaggcata ggtttagtgc
     5881 ttattgtctc tcgagaggag gcagataaag cttcccgtat attagaaaaa gaaggagaaa
     5941 aagttaccgt gattggtgaa gtaatagaaa gcgataggaa ggtgctggta gttgagtgat
     6001 aaaaaaatag tggtattggt ctctggtaga ggaaccaatc ttcaagcctt gatagaagct
     6061 actcaaagtg gctacatacc gggaaagatt agtctggtga ttagtgataa ccctcaagcg
     6121 cttgccctcc agcgagctcg taaagcttct atccctactt taattttaga ttataacact
     6181 tttcctcata agagagctta tgaagatgcc cttcttcaag tgttggaaaa agagagtcct
     6241 tatcttattt gtttagctgg ttatatgcgg atagtgggaa agaaaataat tgctcagttc
     6301 cagcagcgga ttatgaatat tcatccttct cttcttcctg cttttcctgg gttagaagct
     6361 caaaagcaag ccttagaata tggagttaag gtgagtggat gcactgttca tttggtcgat
     6421 gaagggatgg ataccgggcc aataatttta caggcttctt gtccagttga agatgaagat
     6481 acggtagaat ctttatcgga acggattttg cagtatgaac accaaattta tcctcaagca
     6541 gtcaaactat ttctggaaga taaattgaac gtggtgggac ggagggtatt cgttagaaag
     6601 gggtgaaaaa attgaaagtt atggtagtag gaagcggagg aagagaacat tgtttagctt
     6661 ggaaaatatc tcatagtaaa ctggtagata agatttattg cgcgccagga aatggaggaa
     6721 tgagtgaaat tggagaatgt gttccctgga aatctctctc cgacctggtt tcttttgctc
     6781 aagaagaaaa aattaacctc actcttgtgg gtccagaagc tcctctatct cagggcattg
     6841 tagatctctt tgaagctcat tctcttccca ttattggccc agaccagaga ggggcgcttt
     6901 tagagtccag caaaattttt gctaaaaatt ttatgaaaaa ataccaaatt cctacttctt
     6961 cttttgagac ttttgatgat tatgaggaag cagtagatta tcttaaaaaa agagacgcat
     7021 atcctttagt gattaaagct gacggtttag cggggggtaa aggagtttat gtcgcctatg
     7081 atgtagatga ggcgttagat gctttaacgg aacttatggt ggacctaaaa ttcggtggtg
     7141 ctggagagcg gatagtaata gaagattttt tagaaggaga agaagctacg ctaatggtgc
     7201 tctgggatgg taaatcttat tttcttcttc cttcttctca agaccacaaa agagtgggag
     7261 agggagatat tggtcctaat actgggggga tgggcgctta ttctcctgcc ccggtagtag
     7321 atgaaatagt acgggaaaaa atagaaaaag agattgttca tcccctttta gaaggtattg
     7381 aaaaagagaa attaagatat cggggagtta tttatctggg tgttatggta gataaagaaa
     7441 aagaaccccg ggttttagaa tttaatgtgc gtttaggtga ccctgaagct caggtagtgc
     7501 ttccccgagt gaacaatgac tggttagaag tgaatctggc catctgggaa ggaaagctgg
     7561 ataaaatcaa attctcggta aatgattctc ctatgttagg agtagtttta gccagtaaag
     7621 gttatcccgg taattatgaa gtgggtaaaa agatttctgg tctagaaaaa ttccccaacc
     7681 gggtagatga tgaggtctta gtttttcatg ctggaacaga aaaaagggcc gatggttttt
     7741 atactgcagg gggaagagtt ttaaatgttt gcgctcgagg caaaactttg caagaagcca
     7801 aagacaaagc ctaccgagca ataacagaaa ttcactttga gaatatttat tatcgtcgag
     7861 atattggctt tcgagccata ggagagaagt aaaatggctt tagtatatgt gatatgcgga
     7921 agcaaaagtg atttagaata cggaaaagag tgtaccagga tacttgataa gttgggtatt
     7981 gagagccaaa tttttattgc ttctgctcac cgttcaccag aaagagtgga tgaaattgta
     8041 gaaaaagggg aaagagaagg agtaggggta attattgctc ttgctggttt tgccgctcat
     8101 cttcccgggg tgatagcttc gaaaactata attcctgtga ttggagttcc tattaattct
     8161 tctcctcttt tggggatcga tgctctctta tccattgttc agatgccagg tggagttcca
     8221 tgtgctggtg tggggattgg gaaatcaggg gctcagaatg gggcgatttt agctggggag
     8281 attttagctt tacaggatag taaaataaaa caaaagctac aagaattaag gctggagcag
     8341 aaaaaagctg ttgaaaaagc taatgaagaa ttgttaaaat aatagctctg ttttagaggg
     8401 ttttttatct aagtttcaca gctattttca tcctggaaac taaagtttcc aggatgaaaa
     8461 tagaattcga agtagctact tcaaagattg tgtaggtgta taaatatacc tggagtggct
     8521 ttggcattaa agaagttcat accacttcag cactcacagg cgagaggaat tgactcatat
     8581 agaggtcata ataaaaacca cgcgctgcta tgagctcctc atgattgcct tgttccacaa
     8641 ttcggccctg gttcataacc agtatagtgt cagcatcacg tatggtggac aatctatggg
     8701 ctatgacaaa actggtgcgg cctttcctca atttggccat agccttttga ataagcattt
     8761 cagtgcgggt atccactgaa ctggtggctt catccagtat tagtatcttg gggtcggcta
     8821 aaaaggcacg ggctatggtc agaagttgtt tttgcccctg agatatgtta gaagcatctt
     8881 cagtgagcac agtgtcgtat ccctgaggca aggttcttac aaaatggtct acgtatgccg
     8941 cctgagcagc tgctattatc tcttcctcag cagctccttc cttgccgtag gcaatgttat
     9001 ttcttatagt gccgttgaat agccaggtat cctgtaacac cataccaaag attttacgca
     9061 ggtcatctct cctcatatcc cggatatcta caccatctat ggtgatgcgt ccgctgtcta
     9121 tctcataaaa acgcatgagt agattaacca gagttgtttt ccccgcaccg gtaggaccca
     9181 ctatagccac agtttgtccg ggatgggcgt ccagattcat atcctctatt aaaggagtat
     9241 cgggactgta gctgaaatac acgtgctcaa acttcacatg tccctttacc tcttccagca
     9301 ccactggatt atcagtatcc ggtatttctt cttcctcatc cagcaattca aacacccgct
     9361 ctgcagaagc tacagtagat tgcaggatat ttatgatgct ggcaacctga actacaggtt
     9421 gagtgaattg cctggaatac tggatgaaag cctgcacacc acctaaagtg atgagaccgg
     9481 tagcgacgcg tattccacct acaatgcata ttaccacata atttacgtta tttaggaaat
     9541 ttacagccgg acgtattatg ctggatataa attgagcttt gaagctggct tcataaaccc
     9601 tttcattttc tctatcaaaa cgctctttga ctttatcttg ctggttaaat acttcaatta
     9661 cattatgacc agtgaatgac tcttctatat gaccgtttaa agtaccggta ctatcccact
     9721 gagctataaa gtatttttga gaccgtctga taacaaacat ggtgatcaga agagatagcg
     9781 gtatgataat aagtgatatc catgatagaa gcggacttat ggaaaacatc ataattaaca
     9841 cacctacgac tactgctatc gcatgtacca cctgggttat actctgctgg agggtgtgag
     9901 ctatattatc tacatcgttg gttacccggc ttaagatatc gccgtgagaa tggttgtcat
     9961 aatattttaa aggcaaccga gcaagtttgc catctacatc ccgccgcagc cggtatacag
    10021 tgttctgggc tacaccagcc atggtatatt gctgcaacca ctgaaagaag gcagccagaa
    10081 gatacacgac caaaagaata attagtatat gcagcaaggc attaaagtct acaccctttc
    10141 ctggtgtcac atccattccc gatatcattt ctgccagctg atcctgtcct tgggctttta
    10201 actcagttat agcctggtct ttagtcgtgc cagccggtag ttgcttgctt attatccctt
    10261 caaatagttc attggtagct tcacctatta tttttggtgc tgagatagtg aatgtcacac
    10321 taagagcagt aaatattatc accagtagta gcggcatagc ttgaggtttg agataagcta
    10381 taagacgctt aagtgttccc ttgaagtctt tggctttttc tgctggcatc gcagcagctc
    10441 gcccaccacc aaatccacca agccgtcttg ggggctgagc tttcaaattg ttattttcgt
    10501 tattatttac actcatgcta cttcctcctc agaaagctgg gaatatacta tgtcacgata
    10561 tacttcacag gtttccatga gctgggcgtg agtaccgatt ccagctatag taccatcgtc
    10621 attcaaagta ataatttggt cagcatccat aatggtactt acccgctgag caacaataat
    10681 cactgtagaa tggacagtct cttttttgag tgctaaccgc aactgagcat cggttttaaa
    10741 gtctaaagca gaaaaactat catcaaacac gtatatctct ggttttttaa ccagcgctcg
    10801 agcaatagcc aacctctggc gttgtcctcc ggaaaagtta actcctccct gttccaccgg
    10861 ggcataaagt tgaccaggta tctccattac gaaatctttg gcctgggcta cttctaatgc
    10921 atgccagagt tcttcatcgg tagcattctc attaccatag cgcagattgt cagctatggt
    10981 tccactgaaa agaaaagcct tctgaggtac gagccctatt cggtctctta aatctcgcaa
    11041 gtgcatgtca cgtatatcta cgccatctat ttttatactg cctccggtta tatcataaaa
    11101 gcgaggtatc agattcacta aagtactctt tccacatcct gtgctcccga ttatggccgt
    11161 tgtctttcct ggtactgcta taaatgatat atcccggagc actgactctt ctgctcctgg
    11221 gtagctaaac tccacattat aaaactctac agttcctatt gatttgcgag gctctactgg
    11281 tgtagtaggg tcctttatcg aaggtttggt attgagcact tcttgtaccc gttcagctga
    11341 tgcctcaacc ctgggcacca ttacaaacat actcatagct aacattattg atatcattat
    11401 ctccataata tatgtcaaga aggcagttag gtttcctatg ggcatagctc cactgtctat
    11461 tctgtagcca ccaaaccata ttatagcgac agtagacata ttcataataa ttataagtga
    11521 cggtatagct agggctataa tacgatttac tcttaatgtg gtagtggtaa ggtcgcggtt
    11581 agtctcatca aaacgtttag cttcgtaatc atttcttaca aatgctctta taactctaac
    11641 acccataagt ttctcacgca taatttgatt tactcggtct atttttattt ggatagaacg
    11701 aaaaagaggt gtggcttttg atagaataat ccatactgct acagccataa caggtattat
    11761 aacgattatg gaagaagata acaccacatc ttgacgtagg gccattataa caccacctat
    11821 acacataaga ggagccataa ccataaggtt taagcccaaa agcactaaca tctgcatctg
    11881 ttgtacatca ttggtattac gggtgattag cgatggcgta ccgaatttat caaggtcagc
    11941 ttgcgagaaa ctctccactt tgtgaaatat agctcttctc atatctcgcc caaaactcat
    12001 agatatctta gagctaaaat aggaagaagc tatagcacag gctgccagaa gtaacgttac
    12061 aaccagcata aaactaccag tctttaaaat atatctgata tcgcctttag ctaccccatt
    12121 gttgatgatg tcagcattca gattgggtaa ataaaggtta gacataacct gcaacaacaa
    12181 taatactaca gtgagagtaa cctgcttcca gtaaggcttt aaaaatttta ccagtaaatc
    12241 tttcatataa atgcattccc tttcattgtt agagtttacg ttctatatta gagtttataa
    12301 tatccaacaa acgcatgaga ttacgttgct catcatggct taggttctct aagctggtat
    12361 ttataatctc ggctaaaatg ctattcaaca catttaatgt atctaccccg gcattagtta
    12421 ggtaagtatg actcaaacgc tggtcacgct catcctcttt acgtattact aatcctgctt
    12481 tttctaactt ttgaagcata agagttacag tagggggtgc tacgtgcatt ttttcggcca
    12541 ggtctcgttg actgatacct gaattttggt tgataaacca caggcacacg gcttgcccgg
    12601 gataaatgcc cttctccgag gttagcttga ataaaagctg actatggaaa cgcataaatt
    12661 tagaaagaga ctgaaacgct tgaagggaaa tggggtctat tccttctaag ttcaaaaaat
    12721 ttaccttact tgattcttta ttcagcaata aataaaacct cttctccaca agtaaaacat
    12781 ttaactaact aaaggttagc aagcaaacta actgatagct attatactac taatttttct
    12841 cctatcaatt aggctggtaa aaagaataat aaaagggagt aactctttat aatcccttca
    12901 aaaaggcagg cagtctacga cgtaaacttt aaaggcaaaa gagataagaa caataagggg
    12961 gtaaatcccc ttataacccc caaaaagcaa aagaaaaaat gaaaacttaa aaagcaaata
    13021 aacaaatact actggtggaa aaagctccac cagtacgctg ggggaaatcg tcccagcgtg
    13081 cctacggcgt aatattactc ccaccttact gtttgtatat caggaaagct ctgaactttt
    13141 tgcagcaaag aagaaagatc tacatgagga ggagcagtta ctttgaaaga gatttctact
    13201 tcctctcctg atatttctaa ctttatatct actatgttta cttcgtaatt tcctaaagcg
    13261 cttccaatat ctccaatgag tcctggccta tttttggctt tcacggtaag caatcttcct
    13321 gttccgtgtt ttagaatttt agcttcaaaa gagcggagga aaaccaaaac accataggca
    13381 agaagagtag taactatagc ggcaaaatat tctcctaccc ctatagcaat tcctactccg
    13441 gccataagcc acattgctat cgcagtggta atacctcgta gtgctgttcc ttctcgaagt
    13501 actactattg ctcctaaaac tcccatacta gaaactatct gagctgctat tcttcctggg
    13561 tctgaaccgg gtagagtaac ggaaatgatg gttatgagag tagcccccag acaggtcagg
    13621 acataagttc taagcccggc gggtttattt actatttctc tttcccaccc tataattcct
    13681 cccagagcca tggctaaaag taacttaaaa agaatcttta agtactccat ttatttttat
    13741 tatccccggt tgttaatgac cgctaagata aagcataatc aatatattct tctaaaagtc
    13801 ctgctttccc cagaaggtca aggatggtta ctctttttct tacgaaacgg gaaaaaagca
    13861 aagcttctct aatcgttttt tctgaaaaac ctaattcctt tagagtttga tgggcttgag
    13921 ctcttcgcaa cagagaagcc gggtcaggaa taactgatag tttgtcctgt aactgctgcg
    13981 acaaaaaaga agggtcgact atttttattt cttctctgtc ccatttcagc ttttcttgaa
    14041 catctttttc taggaaagga aacaggggac caaaatggag ttttagctct tcttcctggt
    14101 ttttagaaga gcagtttttg cttttcttcc agtctatttc ttctagttct tgcgagaaac
    14161 ggtcatacat tttcttaata atgaccgttg ctcctcctac tctcaggcca tgaagagaag
    14221 ggtctgtttt ccgatgtaga gccatcatct ccagataatg ggcaactaag tgttctgcac
    14281 cagaggcagg gcgagaatct cccaccatga ctatagtgaa accggaatta agaagggcta
    14341 ccgccagagc ataaatggct tcttcctttc tttctcctat ttcttctgct ttttctttta
    14401 atatttctaa agttcctttt accagttccc aggtatattc acagaaatat tctccctgta
    14461 aagtgttcct cattgcccag tcggcgttgg caatgatttt acccaccagg tcggaaaaac
    14521 cagactggat taaagaaaga ggagcttgag ataaaacttc taggtccata aagatagcgc
    14581 aaggaggagc ggcgtcgtag gttgttttat accccccgac taacatagaa gctcctgagg
    14641 aggaatatcc atccatagaa ggagccgtag ctaccacagc atagggaatt tgtacccggt
    14701 gagctacaaa tttagctaaa tcatttatta ctcctgagcc tactgctaat ataaaatcag
    14761 gttcgtggaa aagaaaagaa ccgatacgtt ctcgagcttt ttcatcgggt tccaaatact
    14821 ttagttcagg aggtttttcc agtaagatag gtataacttc aaaagaggct ttttttaaaa
    14881 ttctttccgt ctcccttccc gctgcttcgt aagtattact atcaaaaaca agaaggagct
    14941 tttttcctaa attaagttct gatagtgttt gagggatgcg aggtaacact tcctttccca
    15001 gtatagtttt tttagtcggg atatgatgaa ttttcccgca tttacaaggg aaagaagtgg
    15061 cttcagaaaa aaagaaaagt ttctcggaca ttttttctct ccttacttga gatagttgtt
    15121 tatttcttct aaagaggtaa aaatgaaatc tggctgccag ggggaatttt ttatatcttc
    15181 ttcttttgtc tccccggtga gaactaaaat agtggtgatt cctgcttttt tccccatggc
    15241 aatgtcagtg taaagacggt ctccaatgat ggctacttct tcctttttag ttccagtctt
    15301 ttgcaggata tattctatca tttctggata aggtttacca aatactttgg gagattggcc
    15361 agtagaagct tctatagctt ttatgaaaga cccggcatct attaccggtc cttcttcagt
    15421 agggcagttt aagtcggggt gggtggcaaa aaaagggatt ccttttctta tgaattgaca
    15481 ggcccggttt attttttgat aagtcaggtc agtgtcgaat cctaaaacca caaaatcagg
    15541 gtctttttgc accaggatga agttggcctg caaaaactca atggccagtt cctgagtacc
    15601 caggagatat actcgagaat gggggttcat ttgagagagg taaaaaatag tggcctggga
    15661 agaggtaatt attttatggg aagggaaatc ctgcagcccc atttttctta atttctgagc
    15721 ataaaaagca gaactgtgag aagagttatt agttaagaag caaaaatcct taccctgttc
    15781 agaaatggtt tgtaaaaatt ctctggcacc gggaagaaga tgttctccta aataaaaggt
    15841 gccatccata tctaagatta aaaaacgaat agcagaaaat ttcataaaat tcctccccat
    15901 tatcatcttt aatgagtata atagataaag ggtgaactga aaacaaggta ttagagttca
    15961 aacacaaaaa tgaaaaagag atgtgttatt tttataacac tactggactc caaaattcaa
    16021 caatttaaaa actaaacatg gaggtaaata aatgattcga ataggagtag ataagcttaa
    16081 atcagggatg atagtgggtt acccgataat gagagaagat ggagtggttt ttgtcaaaga
    16141 cggcgtagtt ctgaatgata ctttgataaa tagactgaag aaattaggtt tcgagagtat
    16201 ttacattaga gattctcgtt ttcctgatat agacctggag gaagtcattt ctcatgacct
    16261 ccgccagcaa gctttgatga ccttaaatga gactttttat caggtgataa aggggaagga
    16321 agtttctata gaaccggtaa aagagctggt agatggaatt atagaagaaa ttttaagttc
    16381 taaaaagtcg gtggtaagtt tggttcagct tcgtcaacat gatgattcag tttttgctca
    16441 ttcagttaat gtggctgctc tttctgcttt tgtcggtaaa tttctcggtt tttctcactc
    16501 tcagctaaaa atgcttgctc tgggttctgt tatgcacgac ttaggtaaga ttaaggtgcc
    16561 ggttgagatt ttgaataaac ctgataaact ctctcccaaa gaatgggagt taattaaaaa
    16621 acattcttca tggtcggcag aattaataac agaaaagatg cccgaagaaa gagaaagtat
    16681 caatattgcc cttcagcacc atgaaaggat ggatggaagt ggttatcctt atggtttgaa
    16741 aggagaagat atccattttt tatctcgaat ctgtgcctgt gctgatgttt atgatgctct
    16801 gactgtagac cgtccttata gaaaaagatt ttcctacgct gaagctttag aatacctgat
    16861 gggtaactct cacagtctct ttgatttaca ggtagttaca gctatggtta gacacattgc
    16921 tccttatcca gtaggagaag tggtgaagct aaccagtgga gaaactgcgg tagtagtaaa
    16981 gttaaacgaa ggcttaccca tccgtcctgt agtgcgagtt ataaaagata aagagggaaa
    17041 tatagttggt aaacctcgag atatagattt aatgaaagag ctaactgtag ctatcctctg
    17101 tagtggtgat gaaactccgg aggattttta ttcttccagt gatgtttctt gttttaagga
    17161 aatgggtttt atcgattaat tcctgataaa attacttcca tcgcaaggaa ggtcattcca
    17221 tattcccatt ccctcccgac aggaagaaaa gggaagggaa aaagagtgaa tgatagttct
    17281 tgcttcctct aacagtttta ttcttcggtt atcttctaaa taaaggctgt tccctctttt
    17341 tttggtatac cagttctcag ttttttgtaa ggagggaaaa gcggtagtta tccttttcca
    17401 ggaatcgggt cgaggtttga aagtagaaac cattacttgg tttatatagg gagaaagaag
    17461 ggacaatatt tctttccaat cttcagtttt atcattaatt tcaggaatga gaggatcaat
    17521 cctcagtata gtggggatgc cagcttggtg aagctcaatt aaagcccggg ctcgctcttg
    17581 aggagatgga gctccctctt ctaaaagaga agtaatatga gagttaaaag tggtaatggt
    17641 gatactcact gccgctttca tagtttttaa gatatctatg tcccggatga ccagtgttga
    17701 tttagtaagg ataaggatag gaaccccttt tttctgacat aatttgagaa tctctcgagt
    17761 gagttttcgc ttttcttcct ccggaggata ggggtcagaa gaattagaaa gagagagata
    17821 tcgggaaggg tctatttctt ccaggtcttt ttctactttt tttaagatgc cgggcttttc
    17881 tcgcacctcg aaagcccggg gaataaaaga agtggtataa caataaaggc agtgatgagc
    17941 acaaccggtg taaggattga gagtatattt gggtggacag ctacaccact ttccctgcca
    18001 ggggtcgaaa ggcttaatta ttcgcattga atttctcctg ctattctata ttataatata
    18061 agtggaaaaa agtttaaatc tctttttttc ctccaatttt ttattctatc agagaggtta
    18121 taacttaggt tagttttatt ttgaccttct gggttaatta tagtattccg taaaactgat
    18181 taaaatgtta aaaactttaa aaagtaaatt atatcgaaaa tcaaaaactc aaggtcgctg
    18241 tgcatagaga aagactttgt ctttctctat ggcctttatt tcttttgttt tttaagtttt
    18301 cttaatcttg cgtcgcagac tgcctttttc tccagcatac tggtggatat tttcccgcca
    18361 gttgtttttg tttatttgct ttttgggggt tataaggggg cttgtcccct tatagttgtt
    18421 gtaatggatt ttgcggttaa ctatatatac tgacttggtg ttctctaaaa catggggaac
    18481 ctcaactcga aattggttga catctatttt tctttgtgat actctcaatt gagaagtttt
    18541 ttatgttaaa tttaacgcac gaagggttat caaattggtg tggtagagaa gaaaaagtta
    18601 aataattgat atgtggtgct ttcggatccg aaaagacatt tcctgtgtta ggtagcttcc
    18661 acatctggag ttgaatgcct gctttaaaca ttgaggctgt ggtacgttag ctttgaaaga
    18721 taatggacgt tggagaaagg agaaattaga tgcaaggtat aaatatggaa gagagaaaaa
    18781 agatactgga tatgtacaaa gaccttagag ttaccgatgt gcgagatgga atggattggt
    18841 gtggttatca tcattatggg agtgttcatc atagtattaa gcctctatgg cgtccttgca
    18901 aggctattgg ttttgcaaga acagctcgat acctacctta tgaaggtcca tctcccaaag
    18961 agacaggtga gaaatatacg gagtggtcaa ggtggtatta tcagaatgtt tgtacagatg
    19021 attggggaaa agatattatt gagggtgatt ttatttgttt agatgtttgc ggaattaacg
    19081 taggactttt agggtctaac aacactttag cttacaaaaa taaaggtgct gttggctttc
    19141 tggttaatgg tggaggcata agagataccg atgaagttat actggagaaa gttcccgtct
    19201 ggtcaaaatt tatttctcaa ccgatggacc aggctcgcat acgatattat gagaaagata
    19261 ttccgatagc tataggggga gttgctatct atcctggaga tttagtggta gcggatggag
    19321 atggagttat tgtagttccg gcaaagatga tatacgaagt gcataaatat gcttaccaag
    19381 agatgaaaaa tgataagatt gccagacgaa aattgtatat agaagggaat ttgcccctcg
    19441 atgatacagt agatacagaa ggcttgaaat aatgtgtagt gttttgtggt ggttattaaa
    19501 cggacacagg agttttatgt ccgtttaata tataaaataa caataataaa gggaggaatg
    19561 gtgatgagca aaaaagttct tttatttctg gtagtagcga tgctatcagc aataatggtg
    19621 ttctcaaccg gcttagcatt tgcagagcaa gtagaaatac gagctgcctg gtggggggat
    19681 accggtcggc atgaattgta taataagatt attgatattt ttgaatctaa gaatccagat
    19741 attaaagtga tacgtgaacc gaccagctgg ttagattact gggacaaatt gactgttcaa
    19801 agcgccggtg gtagagctcc agattttata ggtatgcatt tgcaatttgc tgctgattat
    19861 gtgagaagag gaattttgga accactggag ccctttgttg gagaaggagt tattgatatt
    19921 agcaagcatt cagaagctgc tatatccacc gggacaatgg atggagtttt atatatgatt
    19981 cccatgggtc ttacgacaca gactatggta gttaataact ctattctgga agaattgggt
    20041 gttgagcctc ccaattttga ttggacctgg gatgatgtaa agactattgg tttgaaagtt
    20101 agaggcgctt ttgatgaaaa aggagacaag aataaatggt ttatagcgga ttcgtcggga
    20161 gcttatcaga tatttcgcta ctgggtccgc caaagagggc aggaagattt atataccgcc
    20221 gatggtaata tagcatacac agcagaagat gcagcaacct ggtttactat gtggaatgat
    20281 ttacgggaaa acaacattgt acctgatgca gcaaccacaa cagaatatgc tagggctact
    20341 ctggaagata atttgtttgc cagacagaga atcattataa caggtattcc tgttaaccaa
    20401 tataaattat atcttaatgc tgtaccggga gcaaatttgc ttctggttcg caacccttct
    20461 ttagttgatg gcttaccggg agaatttgta gagggcgcac attttgccgt ttctgctcat
    20521 agtacacccg agaaaaaatt agcagcagca aagttaattg acttctgggt taatgacccc
    20581 gattctatag agttgtacag attggaccag ggagtacctt cgaatcgaga aatggctgaa
    20641 tttcttatga ccatagtaga tgaagctgac cagaaaatca tagagtatgt gaataaaacc
    20701 agtgaaattg ccagaccgat taatttccct cccgcaggag ccagtgaagt taattcttta
    20761 tttgagcaaa tagctgagaa ggtaagattt ggacagttga ctcctgaaga cggtggagaa
    20821 caattagtat ctcaagccca ggatatactt aacaaacaca aatagctaca agtaaaaaga
    20881 aggggggcta agctccccct tctttttatc attccttgag gtggttggtg gaagtgagaa
    20941 aatacatata caatagagaa ggttatgctt ttttaactcc ctggctttta gggtttattt
    21001 tgtttactgc gattcccatg atattatcct tctacttctc ttttactcag tatgatgtgt
    21061 tgactccccc taaatgggtg gggctcagaa attacatcga tatggccaat gacccccgag
    21121 tgcacaaaag tttacaagtt acctttactt atgtaggatt gggtgtacca ctacagataa
    21181 tttttgctct gtttgtggct attttactca atagagatag accgggtatt cgtacctacc
    21241 gggctattta ttatctgcca tccttgtttg gaggtagtgt tgccgtagca atactctggc
    21301 gccaggtatt taacaaagaa ggattgataa atcagttcct ggccctcctg ggaattgagg
    21361 gtaagaactg gatagcttca ccggagacag cactgtatac tttgattgtg ctcctgatat
    21421 ggcagtttgg ctctcccatg gtcatattct tgagtggatt gaagcagatt tccaaagaat
    21481 attatgaggc agccatgata gatggagcag gtaggataag gatgcttttt agcataaccc
    21541 tacctctcct cactcctttg attttcttta acttagtgat ggggattata aatgcttttc
    21601 aagctttcac tccagcttat ataatcagtg gaggaactgg agcaccggtg gattcaaccc
    21661 tgttctatac tttgtacctc tacattaaag gattccagca tttctctatg ggatatgcga
    21721 gcgccttagc ctgggtgtta ctggtcataa taagcatcat gacagctctt atgttcttgc
    21781 tatccagatt ctgggtccac tatgaagaat aggagagaga aacaatggga gaacagaata
    21841 gaagtatagt gatatattat ctttttatta ctttgtttgc cttggtcatg atatatcctc
    21901 ttctgtggat gaccagtgct tcattcaaag aagatgttga gatattccaa agtcccagcc
    21961 tcatccctca aaaccccacc ttcaataatt atgcctatgg ctggagaggg gtttccggat
    22021 atacttttgg tagattcctc ttcaattcct ttctgatttc cttcatagga attattgcca
    22081 atatcttatc ttgctctatg accgcttttg cttttgccaa attggatttc cggttccgaa
    22141 acattttctt tgccataatg atgatcactc tcatgttacc atttcacgtt cgtttgattc
    22201 cccagtacat catattcaat aaaatgggat gggtggacac tttcttgcct ttaactgttc
    22261 ctcggttttt tgctaccgac ggtttcttta tttttctcct ggtgcaattc atgcgcaaca
    22321 tccccaatga gatcatggat gcagcgagga tagatggagc tagcgcttgg cagatctatg
    22381 cccggattat gatgcctctt tccaccccgg ctcttgttac tgtggcaatc tttaccttca
    22441 tctggacttg gaatgatttc ttctcccaga tgatttatat cagctctcct tctaaattta
    22501 ccgtatcatt agccttgcgg atgtttgtgg atgctaccgg agaaagttct tggggagcac
    22561 tatttgccat gtcttgtgta tctttgctac cactattcat catatttatt gtattccagc
    22621 gttttttagt agaagggata atgatgggga gcttaaaagg ataagcctgt aataaaaaga
    22681 ggtgatgata ttgtttcagg gtttatatca tataggaata tataccacag atatagagag
    22741 atcactctcc ttttatactc agatacttga ttttaactta aaatggcggg gtatggtgga
    22801 ccatcctgct atgggtaaaa taaaagtggc cctggtagag cagcaaaact gcaccataga
    22861 gctggtgcag cctaacgacg ctgctttagt aaatgaaaaa gctggaccta ttcaacattt
    22921 agcattgaaa gtggagagta tagataaagt tatggagaga tggaaaagca aaaaaataac
    22981 tgagtttcct cttggagaga tagaggattt gccctccttc gggaagggga taagacatac
    23041 ctttatttat ggccccagcc atgagcgtat agaactggtt gaagagtata cttaagttta
    23101 atattcttta gaaaggagaa tagaagaatg gagtattttg taaccgggaa ggcaggtaat
    23161 cgggtatttg tattaaggct tgatccaggt gatatggtat tggaaagtat aaatgagttg
    23221 attgctaaag aggaaataaa agatgccgca gttatatctg ccatagggac attggataga
    23281 tgtgtattgc atatggtaac cactactgat tatcctccta aggagcattt tgagcgatgg
    23341 gaagatacac ctctcgagct tgcctctata gatggcatca tagctgatgg taaacctcat
    23401 ttacatgctg tagtttcgga tactgaaaaa gcttatgcag gacatttaga gaatgagtgt
    23461 agaactttat acctgggaga aatagtaatt atggaattag ggtctatgga tttaacccgg
    23521 gtatataatg agaaaaaagt tttgaagtta gtgaaaaagt aagcatagta ggatcagaaa
    23581 atcctgaaac tgatcccaaa catgaaaagg agaaattcaa agtgaccgat gaagaattga
    23641 taaaagaaat aaaaaagcat cgtttagctg atctctcaga tggtttagat gctctgggtc
    23701 tagtaaatgt aggtaccatg gatccccaaa tgaggcctat ccgggagggt atcagctttg
    23761 caggttttgc ctatacggtt aaacttgttc ctgcccaaaa gagtgttaag gcctgtaata
    23821 gtatagagga atatctcaag gagttggaca gttggtgttc tgatgcttat gcctttatga
    23881 gtgggattac tgaagaaaca gctaaaaata tggtggtggt gattgatgaa ggtggttatc
    23941 ccggaggagt gtggggttct gaaattgcca tgaataccat gaagaaaggt gtagttggtg
    24001 cggtaataga tggggcctgc cgggatagtt atgagtgtaa tttagaaaag gtaaatgtat
    24061 tttgcaccaa acgtaccttt aatcatgtat atgggcgctt gaacaatggc ggagttaata
    24121 tccctatacg gtgtgctggt gtagcagtaa atccaggaga tgttatctgt gctgatgatg
    24181 atggagtatt ggttattcct cgggacaaag cagaaaaagt gttagaattt gctgcagtta
    24241 ttcataaaaa tgaccaagaa acaagagcaa gacattatag agaccttggt tatgaatttg
    24301 attcaaccct aggagacatt gaaggataat aaaacatctg ccagctttac attatttata
    24361 gtaaaaacat cttttctaca taacatttaa tgttaagtta aagagcttat agaactaaaa
    24421 tgcgagtaga ccctgtaagc tgggcatagt aatgagctgt attatggtgg ttattaaacg
    24481 gacacaagag ttttatgtcc gtttaatata taaaataaca ataataaagg gaggaatggt
    24541 gatgagcaaa aaagttcttt tatttctggt agtagcgatg ctatcagcaa taatggtgtt
    24601 ctcaaccggc ttagcatttg cagagcaagt agaaatacga gctgcctggt ggggggatac
    24661 tggtcggcac gcattatata atcagatttg cgatgtcttt gagtccaaat acccagacat
    24721 aaaagtgata cgtgaaccat cgagctggac agactattgg gcaaaattga acgtccaaag
    24781 tgccggtggt agagctccag attttatagg tatgcatccg ttttttgcca gcgattatgt
    24841 acaaagagac gttgtagagc cacttgatga ctatattaaa aataatgtga ttgatgtgag
    24901 caaattttct caaagtgcta ttcttagtgg tacggttaat agtattgttt atatgatacc
    24961 tatggggatt acaatagaca cattatttgt aaataattcc tttcttaaag aattaggagt
    25021 agctactcca gaatttgatt ggacatggga tgattttaaa actattggtt tagaagttag
    25081 aaaggctctc gacgctaagg gagaaaagga caagtggttt atggatgata tatcgggtat
    25141 ttatgaaata tttcgtgtat gggtgcgtca gagaggccga gaagatttat ataccccaga
    25201 tggtagcata gcatatacag ctgaagatgc tgcttcctgg tttgctatgt ggaatgattt
    25261 tcgtgagaac ggtattatac ctgatgcagc aacaacgact gaatacatga acgctactct
    25321 tgaagacagc ttatttggaa ggaggagagc agctgttcgc agaatgccgg tcaaccagta
    25381 taaattgcac tgtgcagcac tacctgatga tgaaatcatt atggtgcggc atccttctaa
    25441 accagatgga gaaataggag aatttattta tggtgcgttt tttgctattt ctgccagtag
    25501 tgcacccgag aaaaaactgg cagcagcaaa gctgataaac ttctgggtta atgaccccga
    25561 ttctatagaa ttgttcaggt tagaccaggg agtgcctgca aatactgaaa tggtagattt
    25621 tcttatgacc atagttgatg aaaaggagaa agctatactg aattatgtta acgaattgat
    25681 gcctcttgcc cggcctatta tttttgctcc tgcaggggct acggagattg attctttgtt
    25741 caagcaaata gctgagaagg taagatttgg acagttgact cctgaagaag ctggagaaca
    25801 actgatatct caagcccagg atatacttaa caaacacaaa tagctacagt aaaaagaagg
    25861 ggggctaaac tccccccttc tttttatcat tccttgaagt ggttagtgga agtgagaaaa
    25921 tacatataca atagagaagg ttatgctttt ttaactccct ggcttttagg gtttattgta
    25981 tttactgcaa ttcccatgat attatccttc tacttctctt tcactcagga tgatgtgttg
    26041 acgccccctg aattttactt atatggaatt gggtgtacca ctgcagataa tttttgcttt
    26101 gtttgtggct atatgtctta ataagagccg atggaagaca gcgaaatatg agaaaagcgt
    26161 gtatccacgg ttgtgggtgt gaaaaaatag tgtgaggctt cacagatatt cggagtgcgg
    26221 gaccagtgaa gtttatccgg gctcgaggaa tataaacaaa caggttggga aatgtaccct
    26281 ggtttattgt atttgaacat tgtctgcatc tacctcgcct gtcacgttat tggtatttgt
    26341 ataaaatgca agctgggtcg ataataacta cgttaaagtt atatctctta atagagatgc
    26401 cagccccggt tatttcaaaa aagcgtaaga aaggagaact ttagagtgaa agaatatccg
    26461 tttggagcag ctttttttat gcccagtgag ggaatagcta acgacctggt caatatgaag
    26521 gctgctggaa tgaatgttgc ccggttggaa tggattaaga atgctgtatg ggaggcgatc
    26581 gaacaatcgc cagatacata ctcgtttggc gtgatagatg aagttatgga agcagcagct
    26641 aatacttccc tgaaggtgat gctgcagata ggtatctacc caccccgttg ggtgagagat
    26701 acctatggtg gaactggtgt agtaaatgaa agaggtttaa cgcctcatcc cagagagaag
    26761 ttttctatat gttatgataa cccccagata attcagctgg ctgagaaata tattagaacc
    26821 atggtacgta gatataaaaa tcaccccgcc ttgtatggat ggatatcgtg gaatgaacca
    26881 cacttgagta atgaagacat tacctgttat tgcccccaca ctatgaaagc atttagaatt
    26941 tggttacaga agaaatatgg taacttgaag actttgaatg aggcatggat agaaggatat
    27001 ccagcatata ccagctggga tgaagtggag ccaccacgac agatgccgcg agaaagagga
    27061 atatatcaag catggcagga ctggcgtaac tttatggatg aaaacttttc aaatcttctt
    27121 aaatgggtaa cctcggttat taaagaagaa gatggttatc accctacaaa ggtaaattta
    27181 ttaatgccaa ctttaaatac tactatcata tgttcagatg tatggaaaat ggctgatact
    27241 gctgatgaaa ttggagtgtc actttttgtt gataccaacg agggtgagta tccccagttg
    27301 ttctcccaga gtgcggattt gattagaagt gcgattgctt accatgaccg acccttctgg
    27361 cttgacgaga ctcagggggg tccaaatttt gtttcacatc gctacccggt acttcctcaa
    27421 gataaaaaaa ttagccttta cccctggcaa tccgttgccc atggagctaa aggcttcact
    27481 tactggatgt ggaggcctct cactattggt catgaagcag gcgagtttgg ccttgttggt
    27541 aaagatgggt cgccaagacc acggacagct gaagctgcca gagctggtgc gataatccag
    27601 aaacatactg acataattct taattctgaa ccagataata aagtgtgcat catgcactct
    27661 caagaaattt ttcacctaac ttatggggag gggctggata cacacattaa atcatttccg
    27721 ttagataatc ctccccaaaa atcgcgctat gccagtgctc ttctgggagc atacacaata
    27781 ctctgggaag aaaagttcgg tgtagatttt atcagccctg agcatattga aaatggaggt
    27841 ttggaaaaat atagagcatt ggtgatgccg tttccgtatt taatgagtga ggataccgcc
    27901 attgcagtta aaaattttgt tgccacaggc ggatttctga ttagcgaatt tccaaatatt
    27961 atgaaagact tgggaggaaa gatatacaaa ataacccctg gtgccggttt acgagatatg
    28021 tttggttttg aggaaattga ttttggacca acggatggca ggccaatagt cctctctgag
    28081 agggctaaag tagcaaccgg tcagatgaaa caaatcgtcc aggtcgattc tcactgcaat
    28141 atcataggac gatttcctga tggaagcccg gcggttatct tcaatcggta tggtcaaggt
    28201 ggagttttga gtttttgcat ggaagtcttt cagccggtta tgtggaataa tcatgatgct
    28261 ctagcaagtc tgattgagaa gtttttacag atttatggaa taactaaggc aggagaactg
    28321 catgggatag aggaaagcct tgcccggaga gtggaaattg ccgaattaac gaatcccaat
    28381 ggtaaactgg tgattgtaat taatcataac caccaggcga taagtggaag tctatatctc
    28441 aatcaaggaa gtaaatatat aaacctggaa aatggtgaag agctgattcc agatcgagga
    28501 aaattgtttc tgaatctgga aggatttggt gtgttagctt tattctgtac tgcttaggca
    28561 ggaagaaagt atagtggcaa aggtctcctt ctgattcctc agctggcctt tgccactata
    28621 gaaaaatgtt aagagaaatg gttctgagct aataggtgtg gaagcattct ttttgtggta
    28681 taaaaagtgt atttctccag tgaacttccc ggttgagcat attgaaaaaa gaactcgcgg
    28741 ttagccagaa catcatatca agttgacatc tatttttctt tatggtactc ttcgattgag
    28801 aagtttttca tgttaaattt aacatataag gagttattaa attggaagta acaaaaaaga
    28861 agcaggttta tcctcgaatt ctcaagaaac ttgagaaaag tgttgagaac ttcactttag
    28921 agatgcctga ggcagaggag aacctcaggc agtcgggttt tgtgtgtaaa acactggatt
    28981 gatgccatgg tggagaatat ttcatttcac gaagaagagc tgaaggattt tgcttcccgg
    29041 gtacgtaaga gaatcattaa ggctacttat aaggcaggag tgagccatat tggtggttct
    29101 ctttcggtag tggaggtgct tacctgtctc tacaatggtg ttttgaatat aaaccaggaa
    29161 aatcttctct ccagaaagag ggatcaattt atcctttcca aaggtcattc agctttagct
    29221 ctttactcgg ttcttgctga gaagggattt atggaagaga cactttttga agaatatggt
    29281 gttcgggagg aaactatttt aggaattcat cctgaacttg acgtgccggg aattgatgcg
    29341 gctactggtt ctttaggaca tggtctggcg attggcattg gtcaggcatt agcttttaag
    29401 atgcaaggcc tggattacaa agtatgggtt ttaaccgggg atggagagtt taatgagggt
    29461 actaactggg aatgtcttcc tcttgcactt cgttatcagt tggataactt ggtgcttatc
    29521 gttgatcgta accgactgca gctttctgga tttactgaag aactacaccc cctggatccc
    29581 ttagatgaaa agctgagagc ttttggttgg tcagtagaga tggtggatgg ccatgatttt
    29641 cccatgttat ggaaacttct ggtaagaatc aaagaatacc ggggctgtaa acccacggtg
    29701 attatagcaa aaacagtaaa gggcagggga gttagtgagc ttgaaaacaa ggtagattct
    29761 cattactgtg gtgtaccgcc ggaagtggcc gagaaatatc tgggggtttt gcctgatgaa
    29821 tagaagagat gcttttcagg aagttatgct cgagcttatg acggcgggga agaaaatggt
    29881 ttgtatttct gtggattcgg cttctcgttt taaagaggta aaagcccgtt atcctgaaag
    29941 atttgtagaa tgtggaatct gtgaacaggt aggtatgagt gtatgtgccg ggtttgcgct
    30001 ccagggaatg ataccggtga tttctggata cgctactttt ttgaccatga gagcttttga
    30061 acagatccgt accgatattt gcaagtggtc acttaatgta aacatttgtg gcactgatac
    30121 tggtttctct cctcaatatt ctggttttac tcatcaggct ctggaagata ttgcccttct
    30181 ttcttctctg gataatatta cagttgctga agcctgtgat taccaggatg ctcttttgat
    30241 gagtaaatac ctttttggag aagtagaagg tccggtatat cttagatttg gtgctcgagg
    30301 taaagaatgg gtctttggcg agtgtcatca gcctgttaga gcagggtatt ttgagccact
    30361 ttcaaaagaa aattttgatg gggctgatgt aactattata gcgctgggac aactagtaga
    30421 ggtggggctg gaggttgctg aagtcctggg gggaaaaggg attaaaactt ttgtgtttaa
    30481 tgcacgtttt gtaaagcccc ttgctcaaaa gatgatagaa gaaatcgctt ccggttcaaa
    30541 actggtggtt agcattgaag agcattctgt aaccgggggt ttgggagaca atttgttgag
    30601 ggcttttcac gaggtgggga gagtgcctcg acttttgaag tttggtgtgc ccagcaactt
    30661 tggcatttcc gggaaaagag actatttatt gcgcaaatgt ggcctttcag tagattcgat
    30721 agcgaatcga gttttgagtg tgttctaaat ttataaatat tattatccgt agtaagaaag
    30781 gggtgataga aaaatcacaa agcttaaccc cagaagatct tgctactcaa cctgaccgtt
    30841 gaagagtaga agaattaaaa aatgttcagg attaccgttt atacttgtca tccatgtgat
    30901 tgtggactcg gcctccagta attggtgaag aactttataa tcagatataa gagatttgga
    30961 gctattcagt aaacaaattt ggaggtgaac cagcagtgaa ttttacgatt gactataaaa
    31021 gattgtacca ggtcagactg ttgtttcttc taaagaaggc aaaataaaat ctaagtacct
    31081 aaaatttaga ttattgtcgg tgtttacctt caacctctaa aggtttaaaa ggatagctgt
    31141 ttatgttttt ttgaggtgtt ggaaggaaat ataaacaaat tgaacaggag ccaggttatt
    31201 tgttctcggt accagtagcg aatttagaag ccttaccagt gaagataagc tacagattgc
    31261 tgaaatggtt gcaaaggctc cccgggaaag aattttgatt ttggcaggtt cggcaagctt
    31321 cttcttcccg gttatgtttc ttggggggtt tatattctta tcggccttta gtgtaattga
    31381 gcggtactga cagaaataac ttaaaaagta ccttaccaat tttaagttct caactttatt
    31441 atggtatagt tattaaacat ggcttcaagc aggggatgag gtgtgatatt atctgtggaa
    31501 gaaaaggttg agattcaaaa ccaggaaata gatatgatgg ttaaagcagc agaattgtac
    31561 tacgtttctg gcttgactca gggagaaatt gctaaaattc ttggagtttc tcgccagaaa
    31621 acgtttcgtc tgatcaagaa agcccgggag atggggctgg tagaggtgaa aattcgaaga
    31681 ccatcagaaa cgcatgaaaa attatctttt gagctgcaaa aaaaattctc tttaaggggt
    31741 gcgcttgtag cgaaagcata tagtagcact ttagatagta ttataaaagc aatagctcaa
    31801 agtggcgcag gctattttag ggaacagcta cagccttaca atacaatagg tgttgcttat
    31861 gggagaactt tacacgaagt aatacaatat tttcccaaaa ttgatgtgcc aggtataaaa
    31921 gtggttcaga tgatgggagc gtacggaggg ataaggtggc aaactcttgc cattgagttg
    31981 attaaaactc tggctgataa gttaggggga gaagcagtat atctttttgc gcctgctttt
    32041 gctgagagtt ccgatattcg tgatgcattt ttaagggaaa ggagtattca ggaaactttg
    32101 aaaatggcgg aaagtgtaga ccttgctctg gtgggaatcg gagggattca gcgtgaggcc
    32161 agtttactca tagaaaccgg ggatttgaga aaagaggaaa tcgaggagct ggcaagagct
    32221 ggtgctgtgg gaggaatctg cggaaacttt tttaacgagg agggagcaat aattattgct
    32281 aatttgaatt caagaagaat tgcagtgcga cttgaggaat tgagaaaaaa tagcaaaaag
    32341 gttatcggta ttgctggagg cgaagagaaa aaaagagcta ttctgggtgc gttgaaaggg
    32401 cactggatta cccatttagt tactgatgaa aatactgctc gatggctgct agaatattga
    32461 tgattgtttg gtctggaata taaaatcgat aaaatataat agtagggggt gatgaaattt
    32521 tgtcatttca aaaagaggtt aactggttag aagctgggaa aatagcccta gaaactttgg
    32581 aaaatttggg aggggtgttc cttgtttctc aaaataaaga aaacctccgc cctaatgtga
    32641 tgaccattgg ctggttagag ttaggaatag tgtggggaag accagtaatt accgttttag
    32701 ttcggccctc ccgttatacc ttctctcttt tggagaagaa tccctttttt gtaatgagcg
    32761 ttcctcctct ttcctggaag aaagaaattg acttctgtgg tagcacctca ggaagagaag
    32821 tggataagtt ctcccggtgg gggtttaacc ccgttaatat aggtaatttc cccgttccag
    32881 ctattggaga gtgtgaggta gccttgggtt gcgaaataat acaaaaaacc agagtagaag
    32941 ccgggagttt tagtgcttct attctcaata gattttatcc ccaggatgac taccatttta
    33001 tttattttgg cgagataaaa gaaagctgga gcaagggcta attttaatcc ttggttccaa
    33061 aagcttttag aaactccatg ggaagaggaa aaactattgt agtagcgttc tcggctgaga
    33121 tatcattcaa ggtctggaga tagcgcaact gcaaggcaat aggatttctg gcgatgattt
    33181 ccgctgcttg agagagtttt tctgcggcct ggaattcccc ttcggcgcta attatttttg
    33241 ctcttctttc tctttccgtc tctgcttgtc gggccatagc tctctttaat tcttcaggaa
    33301 gagttacctc tttgatttct actgctgata cttttatccc ccagggatcg gttttttcat
    33361 cgattatttt ctgcaacgcc tcatttattt tatcccgatg gatgagaagc tcatctaact
    33421 ctgactgccc cacaacgctg cgcagagtgg tttgagaaat caatgaggta gcagatatat
    33481 aattttctac tttagttact gcgtcttcag ggttaagcac tcgaaaataa actacggcat
    33541 ctacagtaac aggaacgtta tcccgggtga taagagattg cttggggaca tccatggtta
    33601 gtactcttaa atctatttta atcaattggt ccataaatgg tatgacaaag aaaagtcctg
    33661 gtccctttgc tcctattaaa cgccccaaac gaaagattac ccctctttcg tactctctta
    33721 ctatccgaat tgccgaggct aaaataataa cggcgataat cactattacc cagattacta
    33781 aactcatttt tattcctcct ttttaacgat taaagtttgt ccctcttttt gagatacaac
    33841 caccttttcc ccttttttaa ctggattttc cgctttagac caccatagct ctccttctac
    33901 tattataaaa ccttcaggat tcaaatcttc tttggctatg cctgtctgtc cgattaaaga
    33961 ttctttccct gttactactt ttttcttcat tgccctcaga gccataccta gagcaaaggc
    34021 aaagaaaact gttacacaaa tcactgtggt aataataatg ttcagcggca aagttatccc
    34081 tccttgaacg tcaaaaagca ttaaggagcc gaaaaacagc gctaccgcac ctcctgcagt
    34141 cagtatccct attcctggag taaccaaaat atcgaggata aaaagtaaaa gtcctaaaaa
    34201 gataaataga ataccagtta agtttatagg aaaaacggaa agggcgtaga aagccaaaag
    34261 aattaatata gttccaaaaa ctccaggaat aatagctcca ggattagaaa gctcaaatat
    34321 gattcccaga gcaccaatta ctaaaaagag gtaggctaaa gtggggtcta aaaggaaatg
    34381 aagaaatttt tcctttccct tcatctccac ttccaagata tgagggtttt taatccctaa
    34441 tttttctata acttcttctg ggctctcggc aatcccatca atcacacctt tttcttgcgc
    34501 ttctttttca gtgagagaga ggctttccct caccattttt tctgctattt ctggattacg
    34561 tcctcgagtt tctgctaatg ttttaataaa agaagctgaa tcattaacta ttttttcacc
    34621 tacctcttgt ccttctatgg ttacaggatg agcagctcca atagtggtat tgggactcat
    34681 aaaagcatgg tgagaagcca taacaatgaa gcttcctgct gatgcggctc gactaccagg
    34741 aggataaaca taggtaatca ccggaagagg agattggagg atagctttaa ctatattacg
    34801 catagattct tctaatcctc ctggagtatc taaaaggatc aagatggctt gagcttcttc
    34861 tttttctccc tcttctatca agcggacgac aaattgctcg gtgactggat tgataactcc
    34921 ttctaatttg cctattacaa ctttttcttg agctgcgaca aaagaattcc aggaaagaat
    34981 agcaacaacc aataataaca gcaaaataat ttttaaattt tttcttttca ttattttaat
    35041 tttatcataa aattgtagta taatacagtt ccttattaga ggaagaaagg atgaagtctt
    35101 ttgaatatac ttctcgttgc tccgggtacg gataatttgc agcgtaagat gagaattgtt
    35161 cctttccccc aggccagctt gcctcttctt gctgctttaa ctccttccca gcatgaagta
    35221 aaaatcattg atgaacgcct agaaaatata gattttgacg agaaatggga cgtggtagga
    35281 attacagtta tgactgctac ttcccatcgg gcttattata ttgctgatga atttagaaaa
    35341 agagggataa aagtagtgtt gggtggtatc caccctacag ctttacctga tgaagctatt
    35401 cagcatgccg atagcatagt gataggtgaa gcagagggtc tttgggaaga gcttataaaa
    35461 gatttagaaa agggagagtt aaaaaagttt tatagtcacg actgccctcc accgttaacc
    35521 gggcttcctt tctctcgcct ggatttatta cataaaagat atttgttaaa acatgttttc
    35581 cagaccactc gagggtgtcc ccacaattgt ggattttgtt ctgtctctac ctttatggga
    35641 agaagatatc gccatcgtcc agcagaagaa gtaataaagg aaatcagcct ttaccctaat
    35701 tctcgtatta ttggctttct ggatgataat attgtgggta atcctcgtta ctccaaggag
    35761 ctttttcaag ccttaatccc tttccagaag aaatgggtga gtcaagggac tttgaagctg
    35821 gcagaaaatg aggagctgtt gaagatggca gctaaaagtg gctgtattgc tctttttgtg
    35881 ggttttgagt ccattaacga agataatctg agaaagatgc ataaaaactt taatcaagtc
    35941 gataaatata agagcttaat agataaattt cacgaatacg gtattatgat cattggttct
    36001 tttgtttttg gttttgatga ggatgaccct tctatttttc aacgaactct taattttttg
    36061 gaaggtgtca atttagattt tgcccagttt tccatcctta ctcctcttcc cggtacagag
    36121 gtttttaaac agttttcaga agaaaaccgg attttttctt atgactggag aaaatatgat
    36181 tttgctcatg tagtttaccg tcctctccgt atgactcctc aagaacttca ggatggttat
    36241 aattacattt tccgtcaatt ttatccctta ccccgggtga taaaaagaac gatgagaaac
    36301 tggagatatg ctcattattt tctaccagtc agcctttatt atcattgggt gtctcttcat
    36361 cctaaatcgc ttcccgaaga ggtagcttct gctttgtttt ccctgaagga taaaaagcac
    36421 tcccccgtaa tttaaataaa gtttttcacc caatgcaact ttgaagtttt gagattcatt
    36481 cagaagtggg attattaggc tggaacgtat tccctcagcg ttaataatca aaatcttttc
    36541 ctatctccta catctttttt cttgcatcac caattaatct atatgactta ccagagatat
    36601 cttttgcata aacacaccta ccagattggg agagtaaccg caaatataat cacgattgta
    36661 ttcagtaata gctatcaagt tagattgtat tcagtaatag ctatcaagtt atcaagtctc
    36721 tccgtctttt ctttcttata tcccttactg gcctcataaa caatttctct tcgtgtcttc
    36781 aagagatacc tcacttatag cccctccctt aagaatattc tctcttaagg gaataataat
    36841 ttaactttga tttttagttg aggcaacgat accatttcac tttaatttta gttgaggcaa
    36901 ctcgacccct tgacaaattt tgaaaggggt gatatagtat aactgctacg ggttctgagg
    36961 gattagagaa ccaagagagc tgaagagtaa aatctgatgc tcctaaaggg attgttgagc
    37021 tccaatcctg gaagaaaaaa tcgaggttca aagatctgtt agaacccgta gctctttttt
    37081 ttctttcttt tgcctctgtt ggtgattctc ccgtaataaa tatgagcaaa ataaaaaaat
    37141 ggcgaagact atttatctat aagttagagc cgcttcataa ctttacgaag atactataat
    37201 aatattggga aaacaaaaat aatcttcatg gagcacatcc aaaggaggag aaggtatgaa
    37261 aattgcagtt gtgtgtgcat cgggaagagt gggtaggttg ctagtggagg aggcgatatt
    37321 gagaggtcat gaggttacag cgattgtccg taatcccagc aaagcgcctc aagctacaaa
    37381 agttattgtg aaggatttat tcgacctgac ccttcctgat gtaaaggatt gtgaagtagt
    37441 tattgatgcg tttggtacat ggacacctga tactcttttt cagcatagta cttctcttaa
    37501 acatctctgt gacatactga gtggtaaacc aaatcgtctg cttgttgttg gtggtgcagg
    37561 aagtttgtat gttaaccccg agcatactat caggcttatg gacaccgaag attttcctga
    37621 tgcgttcaag ccagttgcct caagcatggc caaggcactt gatgaacttc gcaatagaaa
    37681 cgatgtgagg tggacttacg taagtccggc agctgatttc cgggtggatg gtcctcgcac
    37741 aggtaaatac attctcggcg gtgaggaatt aattttgaat tcaaaaggtg aaagtgttat
    37801 cagctacgct gactatgcaa tagctatgat tgatgaagct gaaaaagaca atcatattga
    37861 ccagcgcatc tctgtggttg gggaatatga ttgactatat tttcacagct gggaagtaca
    37921 attaggacaa cgaacggctt gaatgggaat ggtagtgtga caataagggc attctttagt
    37981 agcaggagaa ggggcttcct ctttgcgttg gaactggtta attgttctca ctaccaaaaa
    38041 taaggtgaag gcaattatta aaaaagaaac cagatggctg atgaataatc cgtagttaat
    38101 ggttaccgct ccggcatctt tagcactggt aagggtcagg taaggtcccg gggggttacc
    38161 tggttttagt accacaaaaa gattggaaaa atctatttct cctaaaagga gtcctatggg
    38221 tggcataatc aaatcggaaa ccagagattg aactaacact ccaaaggcgc cacctacagc
    38281 aatacctact gctaagtcaa taatgtttcc ccgactggca aattctttaa actctttcat
    38341 tattttagac atagaatcat ccctcctttt tttattatac agttagagca taaaatggtc
    38401 aattcttagg gttgcccctg ctagcgggca ctctaggctc attatccggt ttgtgtttta
    38461 gcagtataaa gattgttaaa agttccgagt tttgaaaaaa ggtatttttt aagccaaaga
    38521 ggagattccc gcttacagaa ttatattcat ttctctaaag ccccgagatt cagttttttc
    38581 aggcgaagag gcttaagcta aacagtctcc ctttaagtga agataataaa cggcgtgggg
    38641 tagagagtta tgagcccgac gggctcatag cccggaggtc gtcggttcga gtccgacccc
    38701 cgctaccatt tcttttcaag ctctgttggg tttgccagtt tcaccctcag gtctcttcag
    38761 ttactctcgt ctggcaattg atggtcttta gcttcttttt cctcatttga attaatgttt
    38821 ctttgtagat atgtatctaa tagagagttg aacattttgc cgtgattggg atactttaga
    38881 tgaagtagct catgtaaaat cactttaaat cttacctctt taggttcatt cagtaaagat
    38941 ttatcgaagg ttaatctacc tctacttgag caactagccc attttctttt cattgtctga
    39001 atatgaattt cccgggggtt aactcctacc tctttagcta atattagaac atcttcttta
    39061 aactcttcag gagttgtcat tctgtgctac ctgccttaag tatgtccatt atgttctgaa
    39121 ctacctctga gactttggga atatctgtcg ggtataaatg taatataact tcatagagtt
    39181 tagaccggac ttctctttcg tacctctcac ttttttgcca gtggggatat ttttccaaca
    39241 cttctctcat ttgatttgcc attgcctcag catcttggaa acctttttct ttcagcatcc
    39301 agtaaatggt gaaaatatca gcagacatac ctttttcaat ctgttctttc tgagcagagt
    39361 tgatttcatc gattattttt tttagctctt ccagcgtttc ctgggtggtt ttttgcctgc
    39421 tctcatatag cttggctatt aactcagctc tctcagcaat agaaatcaga taaggggatt
    39481 ggccacctct tttctcaacc gtgagggcaa tgctctttat cagattgaat attttttccg
    39541 tgtcagaggc tttgcttttt tcaattcttc tcaaagtttg ttcgtctatt tcacagatat
    39601 ccaatgtagg tccaattacg ccacttctgg tatgttcctg gacgagcata gcggtctttc
    39661 gggtaaactc cctgtctata ggaatccccc gctcataatt ttctataaga cttttataca
    39721 ttctggaaag tgtttcgtag tcgtttacgt aaggtcttaa aaaggcatcc ggtgaaataa
    39781 tctcataaat agcggatagc tctttgaaga atttataaaa ttcctttctc ttctcttcat
    39841 ctctgaagta ttctagcacc gcttcaaatg ccttatcttg agcttttcct tcgataagag
    39901 taaggtattt ctctctggcc tttttcattt cctccgcaaa tcttttcttt aaaatctcaa
    39961 catcctggac aattccctcg atgtcttggg agtcaaaagc cagagccttt tcaagattgt
    40021 cgaaaatacc aacaaagtcg agaacaaatc cggaggactt ttttcttcct tcatcgtctt
    40081 cataaggcct gtttactctg gcaatagcct gtaaaaggat atggtctctc atgggtttat
    40141 ccagatacat acaatagaga attggggcat caaaaccagt aagaagcttt tcggtcacaa
    40201 tcaatatctt tggtagctca tcgggctttc tgaaagcatt tcttattctt ttttcttcca
    40261 tttctgaaag atggtatctg gatagctcgg gtgggtcgtt gtaaaagggg ctatatacaa
    40321 cttttgaata gtcaggtggt aaatatttgt ccagttcttc tttatacaga gcacaggctt
    40381 ctctatctac ggcaactaca aaagctttat atcccatagg ctcgatatat ttctggtaat
    40441 ggtcaatgat atatctggtg accttttcaa ctctttctct atttttgagc atatttttaa
    40501 gggttactgc cttatccagc actttgttga gttcctcaat atcgctcatt ccttcagctt
    40561 cagccaggtc gaggaattcc ctctccagca cttctttatc aacctgaagc tcgttagggg
    40621 caagggtgta atatagaggg acggttgtgc catcttctat ggattcggca atactgtatt
    40681 tatcgaggta tcccttaggc ggatcatctc taccgaaggt gataaatgtt cctttaccat
    40741 agctggtttt atcaatggga gtgcctgtga aaccaatgta cgttgcatta ggaattgcgc
    40801 ccatcaaaaa atttccgagt tttccgcccg tagttctgtg tgcctcgtct accaggacaa
    40861 aaatgttctt tcgcagattt atattttcaa ccatatcctc gaacttatga atcattgtga
    40921 caatcaagcc tcttctatct cgtgaaagca agtcttgcag atgctttttg ctttccgcga
    40981 cctccacatt ttcaataccg agagcagcga gatttccaaa aagctggctt tccagctcat
    41041 ttctatccac gagcataata actgttggat tttcaaagac agggttttct attatctttt
    41101 gagcagctac tatcatcgta taggtcttgc ccgaaccctg ggtatgccat atcaggcctc
    41161 ttttctttgt tgaatcttcc gctctttcca ctatcttact gactgctctc atctggtgag
    41221 gccttaagat aacttttttg agctcatcat cctgcctggt aaacattaag aaatcaacaa
    41281 gaagtttaac cactctttct ctatctaaga atgttttaac caggctttca aagtcaccac
    41341 tgacttcttc tttccagtta aagaggagct tctcagaagt gttccaggtg gcactatagt
    41401 aatacttcaa gatgtgagtc aaggcataaa cctggagaat agccagaagc tcagggcatt
    41461 ctctgtgata ccttttcacc tgttctaaag cttcgcttat cccctctatc ttataagcgg
    41521 cttttgtttc gattagtaag agagggacgc cgtttacgag gaacacgata tctggcctga
    41581 tagtcttagt cccgttggtg aactcaaact catccgttac atggaaggtg tttctgtcta
    41641 catcttcagt atccagaaat tttatatttc tctcccgctt ttcaccggga acgaatatcg
    41701 ttcctaagcc tttgagatat ttccaggcgg taaaattacc ttcaatgttg ggtagaacag
    41761 tttccagctt tttaattacc tcttcggcaa gaaggtggtc cataaagctg gggttgagtc
    41821 tttgcatctg attgatgaat atctccttga atacgagacc cgtcttgcca cctctcaacc
    41881 ttaaggcttc atcagggcta acatactccc atcccagctt tatgaatatc ttgtttccat
    41941 cctgggaaat atattctgcc gattgctctt ttacatagtt aatgataggt ttctgaacgg
    42001 catatttttc ccctcccaat gagctcatac tcccacctcc aggttattga ctcttatctt
    42061 agcagtcata agattatata aaagagtctt aaatagatcc tccagggctt tctttttatt
    42121 ctcctctgct tcaatcttct catcaatggc agagagcatt gaagcgattt tttgctgggt
    42181 gggaagaggg ggaaggggga tcaagaatgt tttaagtttt gtagcactaa tattagattg
    42241 gcttgctcct ctagtggcaa gacattttaa tctaccttga gttatatccc aattaaaata
    42301 aaaattgaaa agcaaaggaa aaattttact atcattcacc ctcaatctaa ttaagtatga
    42361 tgcaaagaca aaatcatcat tcaatgtgaa aactgatgtt ttcccaacga gttcaaagct
    42421 gttcgttctg ttaaagagaa tgtctccttt ttctaattta aaccttctaa atgtactttc
    42481 atctaaattt acaaattgta aatcatcggt tgaaatcgca ccatttatta aattattcat
    42541 gcgtaagatg gggtatttgc caagctttga ccccctaata gataatccat attgtgcctt
    42601 ctttaaaaga tcataatccc ccagtctcag cacttcccac tcctcaggta tcatcccgat
    42661 ttcggtctcc ttgagcggta cattctcggc atccacaaca ggcacagggc cataggtaaa
    42721 gaggtagttc atcatcgatt ttttgaggcc ctgggcggcg cttatcaccg cctccgtttt
    42781 ttcctttgcc tcttgaacgg cagagagaac agcggcaatc tttcgctgct cgggaagagg
    42841 ggggaggggg aagataattt gtttaatttc agatatattt aatgttggat aaccatccgc
    42901 tttttctttt gcaagtttta attctcttag caagtaaaaa agaaaatctt gagtcgcatt
    42961 cttttcatcg gcgataatag tacaaagatg acttacaacg taagcatcgg ttggaagcat
    43021 cgcccttttt tccaggtaaa tcgtagctcc gctttttgga aaaacaattg ttcctttggg
    43081 aaataatttt agtttataat cttgaactgc tttatcattt attaagtcat attcttttat
    43141 tacgcaatct tcattatcga tgtggctaac tcggataaat ggtttctctc ctttaaaata
    43201 ttcatctcct tgtggagcac tacctcccga cgaaatttca gctacttctc ccagcctcac
    43261 cacttcccac tcctccggta gcggtccgag ctcagtgatt ttgaaacctt ctggtaggtc
    43321 tctatcaagc tcattcatta aatctccacc cccagctccc tgagaacctg ttttagttgg
    43381 gcatctgctt ttcttctttc ttcttcggcc tctttgagtt gcacaaccgc atcctctaaa
    43441 ggtagggtat catcttcacc gttttgagca acatagcgag aagggctcag gttataatca
    43501 tttctggctg cctcttcccg ggtgatagtt ttgcttatgc cctcttcttc tctccattgg
    43561 aggtagatat cagaaatctg ttttatactc tcatcgggga gataattttt gggtctacct
    43621 tttttaaaca gtttagaagc attaatcagc aatatctgcc cttttctctc agctggttta
    43681 gatttattga ttgctatgat aactcctggt gccgtcgtgt tatagaaaag attttcgggc
    43741 agcaataaaa cagcttctac aaaatctcct tcgacaaact cctttcttat atccctctct
    43801 ctatttctac cttcgtttcc gctgcctcgg gaaactgcac cggtatcgat tactacagcc
    43861 atcttacctt tttcatttaa agatgcgaac atatgttgaa tccagcccca gtctgcactg
    43921 ctggaaggag gataaccaaa aatgaagcga ttgtatgggt cattctcata agtagaggga
    43981 ggaaaattct ggttccacat gggattggca gtaaccaggt caaattttct gagcgacccc
    44041 tccggcgtta aaaatgcagg cctcgtcatt gtatccccga gctcaatatg agcttccata
    44101 tcgtgaataa aagtattcat cttagctatg gcaaatgtgg aatgcaatat ctcctgacca
    44161 tagaatttta gaggttcaag agaagaatct gctccatatt tctccttaaa ccgcaaatga
    44221 gattttatca aaagacctcc cgaaccacag caggggtcgt atatctcatc cccgggctcc
    44281 gggttcagga tgtaagacat caatatagcg acttctccgg gggtataaaa ttctccggca
    44341 ctctgcccgg aaccttcggc gaacttccgc aatagatatt cataagcccg ccctaaaatg
    44401 tcagcatcta catcgcttaa tcccagccgg tatctaccta aaacatctat gagctctttt
    44461 aatctatcat cgctgataat tctctgtcct gcagcagtgg cattaaaatc tactgcgtct
    44521 ataacacctt gaagtttgtc attttctctg gcgatagtgc gaacagcatc ggttaaatat
    44581 tctccaacat tggtggtctg tttggcaatt tcctcccatc ttgtcttttc aggtagataa
    44641 aagcggacga ggttatggtc ttgggctaat aactgttcaa caatttccct ggagcagtac
    44701 ttttgggaga gcctatttat ttcatcatcg aatacgtcgg aaagcctttt taaaaatacg
    44761 aggggcagaa tataatcctt atacttgggg gcatcaatgg ggcctctaat tttacaggcg
    44821 gcttcccata gccagccctc caacgttttt acgtcgagct cacctccggg catattttaa
    44881 cctccctacc tgaatgtcca gcctcttgag cttgacaaaa tgaaaaaatg gtttaatata
    44941 atacttgcct taagcaaggc aataaacggc gcggggtaga gcagtcagga agctcgtcgg
    45001 gctcataacc cggaggtcgt cggttcgaat ccgacccccg ctaccatttc ttcttccttc
    45061 agctcctggt tttctcccca gagaagaatt ttctctcccc aagaaaatag ctcatttgtt
    45121 tcttctccta agtcttctcc aattaaacta tctataatat ttccctttaa gaactcctcc
    45181 cagtcttttt ctgcctcttc ttctttcccc agtgcccggt aaaccagagc tctgaagcca
    45241 taagctttat agaagtgggg attaatcttt attgctctgt tgaggtcttc ttgagccagt
    45301 tcgtaatctt tcttctggta ataaactatc ccccttccca gaagagcagg gtaaaattcc
    45361 ggttctaaat caatggcagt ggtaaagtct tctatagtct ctgcatattc tccttgttta
    45421 attcgggcaa ctcctctctg gtagtaagcg aggggaaaat ctttcttaaa agttagagcc
    45481 tgggaaaaat cctggatagc ttgagaataa tctccctggt gaaaaaagca cattcccctt
    45541 aggaagtaag cctgaggatg agaggggtta agctccaaaa aatgatttat atcagccaaa
    45601 acctggtcag aatagcttct caattttaac aagatgcgag cccgggcaag atatacatcg
    45661 gcctcttgag ggtcaatctg ggttacctga ccgaagtctt ccagtgcttc cttgtatttt
    45721 ccctcttctg agtgagctac cccccgattg aaaaatgcct ctttcaattg aggattcaat
    45781 tccacagctc tggttaagtc gctaactcct tgttctatat ctccttttga taaataagca
    45841 ttaccccggt tgttataagc ttcggcgaaa tcgggtttca actcaatagc tttggagtaa
    45901 tcgtcgatgg ctttatctat atctcctaaa tagtagtaaa cgttacctcg gtgagcgtaa
    45961 gctacagggt gatggggtaa caaatttatg atggcgttgt aaacctccag tgcttccgaa
    46021 tatcttttat cccggataag agagaaaccc tggtggaaca attcttgtgc ttcaggagga
    46081 aggggatttg ctatctctat cagcgagaca taaatagcaa aaattgctaa aataatgata
    46141 attgccagaa ccattttttc accccagaag atattttcta aattctacca caaaaattta
    46201 tagtttttct atctggatat tgccctccac tcctaaaaac tttcctaaaa taattaaaat
    46261 cccttgaata tcagtgattt gccgggaaaa ttccagcact ttctttatat cctctttttc
    46321 tcgcactcgg ttggccaaac tggtagccca ggcatcagcc acagctgacg attcggctac
    46381 cacgactaca gcatctgctt taccaaaact taaagaataa ccaactgtcc ctgaagaggt
    46441 agctactccc cattttttgc ccggagataa cttaatacct atccgaaagc taaaaggaga
    46501 atcttctccg gcatacactg ccaccactcg agcttcctga gaaaagacca gtaaatctcc
    46561 accattttca attattacct cctggctttc ttggcggagt tcttctccca ccatttcatt
    46621 tattgcccca gcaacagaag ccataggacc tactccggct ttttttgctg cttctgccat
    46681 catctggcat acaggaggag caaaaggggg agcggtaact ggaactaaag aatgcaaaaa
    46741 atcggggtca taacgtagat aagcttctaa gttatttctt tccttttcta atcttttata
    46801 agctaaatct tccaggtttt tctgtgctag aatgaaaagg tcgctctctt ttatctggac
    46861 agaaaaaggc actaaatctg gtgccctcgt acatttacga taccatctct ctacatattc
    46921 ggtcacaaaa gagcctccat agcatgagtg ggacaggcta aaacacacat ttcacaggca
    46981 atacattgag tttcgtcata tttcaccagg taggaaggct tttccacata caaagcctga
    47041 gtggggcaaa ctcccacgca agagccgcag tgaatacagc gttcctccac cattcttata
    47101 tcctggctta aagattcgac ctctaaccca ttttctttca aaaaacgtaa tccttcttct
    47161 tgcttctttt tctctccgat tatttctatt accataagtc cctcgcggtg aggagaaata
    47221 gaagcgcgca taatgttaaa atgcagcccg aactgaagag ctaactcaca cactaaaggt
    47281 ctatctacca attcaccggg aaaccgcaaa actaccttct gggaagagtt catgttttca
    47341 cctccctgtc tttccattat ttaccggtct ttcgggaaga ttatggaatt gaggtccttc
    47401 ttttcccgga agagataaaa ctggttgagt taaagtgaaa tctccttttt tgatccagtc
    47461 tttcaggatt gaagctattt cccgggctcg ggggtaactg gataaagaag cagtaggaac
    47521 ttctttccct tctatcttta tttttccctg gcgcaactga gcgtagctcg cataacccaa
    47581 tatctcccct gtcccattgg gataagcgtt gctgtaatct ataatgggag ctaagatatc
    47641 ctcatctttt actgcacaat aataaagggt ttcttcatct aagataggaa tgggcactcc
    47701 cagacccacg gccatagagc aaccatagcc tctcatactc actccccaca accaattagc
    47761 attcattcct ttcaaatctc caatcactgc taaagttcct gctccttctt tggggatacc
    47821 tcgttcgttt ctatctactc caggattgtg ttgcgttccc tgccaggcaa catatcccac
    47881 tccccctccc agaaagattc gcgttcctac tccaattact cgataaaaag ggtcattgag
    47941 tagaggagaa agctgtccag cggaggaata attagcgtta cctaatttgg ggagcaagac
    48001 tcccatatag gtgtaaaggg ttttagaaga gaggttcact gccacattat agttctggta
    48061 agcattacga gggttaaaaa ggtaagcttc attcaaatcc ctcaggttaa tccaggttac
    48121 taactttcgc cgggggtaac agtccgttcc gtaactatag gcttcaagaa gcacatcttt
    48181 ccctgctacc aagtcctgaa tcacatgacc tcctccgtat ttaaattctc cagggtggat
    48241 tttgttgagg gggtcatcct gggccacctg tgctgctcca atatagaaat ctacagcagc
    48301 taaaccggag taggccatta ctccattgag ataggcttca ctaactttca tcctgggttt
    48361 ggtgtgccca atattaaaaa aagctcctga agagcacatt actccaaaag taccggtggt
    48421 caccacatct acctcttgag cgaccttttc cactcctttt tcctctacta aatcaattac
    48481 ctcttcggca gtcaccacta ctgctttacc ctgtcgaatt ttttcattga tttcttcata
    48541 agtccggagt ttttctccca ttttctcacc tcctgctatt tcttccttat ataacaaaaa
    48601 acccctcgtc gagggacgag gggttaatct cgtggtacca ccctatttcg ctgacgcaag
    48661 ccagcctctt ttacaccaaa agagtgcagt ctttttaacg gagactaccc gtaaaccact
    48721 acttaatttc gcagtttcaa ctcccaggcc atttccccag gctctggata cctggctccc
    48781 acctacccag gctctctcta atcctttccc tggttactaa tcccgttctc ggtctttatt
    48841 ttattaaaag tgagctaaat tgtataattt ccatcctcgt ttgtcaagca catggtggct
    48901 ctagatagtg tcaactcatt gtaggagtac ttttattttt ccctctgtta ttagcttttg
    48961 agactctctc cattcagtat tagatagtat cataagaacc acccatccag gaaaagtctt
    49021 tagttaactc tttaagaacc taatttcccg gaaaattata actatgtttt tgggtcaatc
    49081 cccagactct c
//
LOCUS       DAOSVE010000003        43228 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_607_length_43228_cov_67.370509, whole genome shotgun sequence.
ACCESSION   DAOSVE010000003 DAOSVE010000000
VERSION     DAOSVE010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 43228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43228
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_607_length_43228_cov_67.370509"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..79)
                     /locus_tag="PK016_00460"
     tRNA            complement(3..79)
                     /locus_tag="PK016_00460"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43..45),aa:Arg,seq:tct)
     gene            complement(124..1818)
                     /locus_tag="PK016_00465"
     CDS             complement(124..1818)
                     /locus_tag="PK016_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01583.1"
                     /translation="METFWGEYQKEIESSFIILEKERVIPRLWEKDYTLWKPSPQEIV
                     NRLGWLNVFQEMQKNVARLENMTDELIKEGFQEALLLGMGGSSLAPDLFKKVWGSKKG
                     FLNLHVLDSTDPEMVNYYAQSLDPGKTLYIVSTKSGNTLETLSFFKFFYNLVQERLGK
                     ETGRHFIAITDPGSPLVQLGERYGFREVFLNSPDIGGRYSAFSFFGLVPATLIGIDLS
                     LLLEKATLASQKCSPSTLLENNEGAILGTALGVLAQKGRDKLTFFFSSPRWESFGDWL
                     EQLIAESTGKEGKGILPVIDKQPGKPEVYGKDRLFIYIEGEKNDSLDNLLEELKDAEH
                     PVIKIPITDNYDLGEQIFIWEFATALSGYWLKINPFDQPNVESTKKFTQEMMRKYKEG
                     SWEEEKPILIEKGFNVFCDFSASSLEEVLKQFLGFVESSSYISIHAYLPVNPPIEKEL
                     ILLASLLRDRTKKAVTFGYGPRFLHSTGQLHKGDGGRGLFLQLVSESSLDIPIPEEAG
                     SDVSSFTFGTLKKAQALGDREALKRAGRKVISLFFSGNSEEKIRALREILLHICKC"
     gene            complement(1862..2284)
                     /locus_tag="PK016_00470"
     CDS             complement(1862..2284)
                     /locus_tag="PK016_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01584.1"
                     /translation="MIEALISFVILLLLVVAVSSLVILWGKLFNSLSQKIEEEENLLG
                     SINTLFQLVRYSDNLQSYSNGRKLTMTLSSNPLEIYSQGGTLLLKNKGVSNPIAEGCS
                     EARFIVEGSEEKPFMRAHLVFGKEGVDIQINPCLLLSP"
     gene            complement(2298..2630)
                     /locus_tag="PK016_00475"
     CDS             complement(2298..2630)
                     /locus_tag="PK016_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXL01585.1"
                     /translation="MKNDEGFTLIGILIALCIFSFLILVVGQEINFIGKTQKSSTIKI
                     KALIDAVNTMEELKNDEVPASGKEEKEEFVIESQVGDFSPEIYELKVMVEDKNGEEIL
                     SLQTLREK"
     gene            complement(2620..3063)
                     /locus_tag="PK016_00480"
     CDS             complement(2620..3063)
                     /locus_tag="PK016_00480"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HXL01586.1"
                     /translation="MKASRENFSSGFTVLELLIVLSIVSVISAFSFPCLLGWQASFLG
                     KLTAQRIASELRLAKIEALRTGVTTQIIFDPVQDFFTYQGCTSKRVNFPKGVDLCTTN
                     FSRHTILFYPTGTPSMGGTVTIRINEKLLHLIITPVTGRVRISEK"
     gene            3471..4052
                     /locus_tag="PK016_00485"
     CDS             3471..4052
                     /locus_tag="PK016_00485"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HXL01587.1"
                     /translation="MSKITNEWLLIVFISCVLFYSYAGIYISRQTGASIPRWPSLIKS
                     RDYTFYIIYICSIALIINTIISLMGEYSLFYLFLIGLVLMFFGIYLNILARKELGRYW
                     SPLSSSDNSEKLIKSGVYARVRHPIYLSNLIFFLGVTLIAGSWYSWILFILFLGGLRK
                     RVKKEEEELYKKFGEEYRIYAKETPLILPRLRR"
     gene            4110..5360
                     /locus_tag="PK016_00490"
     CDS             4110..5360
                     /locus_tag="PK016_00490"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HXL01588.1"
                     /translation="MINIIPVTKIVQKPLSLCTLSSVEKGVIEELNCSGVTIKKGSRI
                     AIAVGSRGISQIHEIVRVVAQWVKDKGGYPFIIPAMGSHGGATPEGQREILESYGIKE
                     EYVGAPIYPSMEVVELPKGNLPNRVYMSKVAYEADGTVIINRVKPHTDFHGRTESGLL
                     KMCVIGLGKHQEALEMHRFGARGLKELIPETAKQIIKYGNIVLGLAIIENSFHQTMLV
                     KALKPFDFEAEEEKLLKLAWENMPVLPVDKIDILVVDQMGKDVSGSGMDTNVIGRMNI
                     DGEPEPEKPHIKVIIVRNLTDESHGNATGMGLADIITENFYRKIDFPSTYENILTSTF
                     LERGKLPLIAKNDRQALEWALRASRIIDPEEIRLIRIKDTLSLNQMYVSSRVLSEIKE
                     CNSIEVTEDFKDLLTPEEEELKDF"
     gene            5367..6635
                     /locus_tag="PK016_00495"
     CDS             5367..6635
                     /locus_tag="PK016_00495"
                     /inference="COORDINATES: protein motif:HMM:NF018682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HXL01589.1"
                     /translation="MNKPALPLLVIADDATGALDTGVQFSKQGFPTLVVPDNQLPLRN
                     IEANLQVLVVDTNTRNVSPGEAYQRVKEVVELALSRFEIQSFYKKTDSTLRGNIGKEF
                     EALLDVTGGKELFFIPAFPRMGRITRGGYQLICGKAACQSDLAQDILNPAKEGYIPEV
                     IQKQSSLSVEVVNENRRNVFLKYNSKRNRRMIIVLDAKNDQDLKEIGLILQREGKIFL
                     TAGCAGFAEYLAQFLDLQIAKSRVFTPPSQWLVIGGSLHPLSLEQLDFASQKGIPQVI
                     LPPTFFFEDNIEGFFQKICYYFQRDPGFIIRTVKSRKDLEEYRELAQKEGWEWEKVPS
                     LISQKLGNLVKELIKRSDERRTIIVFGGDTTRGVLESFSWKRINPGEELLPGVPLSQV
                     ITEEGEELYLITKAGAFGEKETLVKLINVL"
     gene            6646..7611
                     /gene="pdxA"
                     /locus_tag="PK016_00500"
     CDS             6646..7611
                     /gene="pdxA"
                     /locus_tag="PK016_00500"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013386537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HXL01590.1"
                     /translation="MFIGVTMGDPNGVGPEIILNSFRKGETGKDFVVIGDYQVMDFCS
                     RLLKYQVPLKKIVNLSQWEKGRLNILDLGLLEEKDIEIGKVSSKAGFAAQKYVERATL
                     MALDGKVSALVTLPVNKKAIRLTDPTFTGHTEFIAQLCGQSNYAMMLTSEKLKVVHVT
                     THIPLTEVAKFIKKNRVVEVTTLAGEAMQMLLGRKPKIAISGFNPHAGEDGAFGREEI
                     EEIIPAVEEVRSKNWEVAGPISPDVVFLEAYRGKYDVVIAMYHDQGHIPVKLLDFEGG
                     VNVTLGLKIVRTSVDHGTAFDIAYKGVANIKSFVQAFDLAEKLSK"
     gene            complement(7664..8932)
                     /gene="hflX"
                     /locus_tag="PK016_00505"
     CDS             complement(7664..8932)
                     /gene="hflX"
                     /locus_tag="PK016_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HXL01591.1"
                     /translation="MEEKRGILILVDRGNDSSFSSREGERKETEEVAWSAGWKILSVV
                     ESRVKNPFYKYYLGKGKVEEVKSLIEDNPEINFILFNVEITPSQQRNLEKFWQVPVYT
                     KNALIHEIFAQRARTAQGKIKVELAQLHYQLSRLTGKGRDLSQTGGGIGTRGPGEQKI
                     EVERRKIKDRIANLNQELRKIERQREVQKNLRRERKIPNVALVGYTNAGKSTLLNALT
                     GSQVYVEKKMFATLDPTTRKLFIPGIGEALFTDTVGFIREMPPTIKDAFRATLEEIKD
                     ADLILEVIDISDEDYYLHFQVINQILEELEINNSPRILVFNKIDLLCSPLILERDIKE
                     NYTYVFVSAQELRGLKELKEKISSSLQHEVRHLCLQVNYSQLPLVQGAIYHQGYIEDL
                     KTTKNGEVMIKCVVRKEAADKICHLSLARV"
     gene            complement(8936..9586)
                     /locus_tag="PK016_00510"
     CDS             complement(8936..9586)
                     /locus_tag="PK016_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012447895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXL01592.1"
                     /translation="MDVNTKTTAIIAAYNEEKTIGPIIDVLKAIPFLNQIVVVSDGST
                     DHTVDIARSRGVEVVELLYNVGKGGALYQGLMNTTSNVVLLVDADLIGLTPQHVIDLV
                     KPVIDEEADVTIGIFEDGRFATDFAQKIAPFLSGQRAIRIDILRNIPDIEVSRYGVEV
                     AINRYIRKNGVRVKLVKLPGLTQVMKEEKLGLVKGLRERMKMYRDIIKNFYKGGNR"
     gene            complement(9590..10840)
                     /gene="proS"
                     /locus_tag="PK016_00515"
     CDS             complement(9590..10840)
                     /gene="proS"
                     /locus_tag="PK016_00515"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HXL01593.1"
                     /translation="MSLMFVPTLKENPQEAEIASHSLMLRAGMIRQLSSGVYIFLPLG
                     LRTLNKITRIVKEEMDRAGGQELLLPALHPGELWKETGRWEIYGPELIRLKDRKDRDF
                     CLGPTHEEVITDLARREIHSYRQLPLLLYQIQTKFRDEMRPRFGVMRAREFIMKDLYS
                     FDRDFEGLRVSYEKMYNAYCKVFSRCGLSYKAVPAESGVIGGEVSHEFLILADSGEEK
                     FSVCPSCDFSTTQDEEKCSNCGANLEEKRGIEVGHIFQLGTKYSEPMKAYFVDKDGEE
                     KPLIMGCYGIGIGRTMAAAIEAYHDEKGIQWPISISPYEVEIIPVNWKKVEQRKAAEG
                     IYQELKEKDFEVVIDDREFSAGYKFSEADLIGFPLQVIVGEKMSQEGKIEIKVRHNNE
                     RWETKREEVLTLLLKLKEELAPKA"
     gene            complement(10843..11913)
                     /gene="ispG"
                     /locus_tag="PK016_00520"
                     /gene_synonym="gcpE"
     CDS             complement(10843..11913)
                     /gene="ispG"
                     /locus_tag="PK016_00520"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HXL01594.1"
                     /translation="MKRENTREVSLGKIKVGGKNPIWVEAMGRESPAKVKESIEEIEK
                     VIEEDCEIFRLAVPDEESLLGLLEIKRRIDIPLIADVHFNLDLGIKAAQLGIDAVRVN
                     PGTAGNKKKLGELIKILQDKNLALRIGANTGSLPLHYRGKNRVEALFGSLEELVSIPE
                     KNNFKNIILSAKSTDVEETIIIYEKLSRAFPYPLHVGLTEAGEGGEGIVKSSIALGII
                     LREGIGNNIRVSLTATDPVLETRVAWSILEVLKLRSKGLEIISCPTCARRKGDVVALT
                     RDFKQKVRERKIKIVGKVAIMGCEVNGPGEAKEADWGLAFAPQEKALLFSRGERIKIV
                     RQEEAEEELISLIIKEMEEKGP"
     gene            complement(11910..12962)
                     /gene="rseP"
                     /locus_tag="PK016_00525"
     CDS             complement(11910..12962)
                     /gene="rseP"
                     /locus_tag="PK016_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HXL01595.1"
                     /translation="MIITILAFIFVISLLIIVHEFGHFIMAKWFGVQVLSFSIGYGPK
                     LVSFTRGETEFSIRLIPLGGFVKMGGMEGNVIEGYEEANTPPEKRFDHKPLWQRSLIV
                     AAGPIMNIVLSIVLLFIVFTVAGIPTGNLKVQEVVAGGPADQAGLKAGDIILEVNGKS
                     ASDLDEVVSVISSHPGEEIELTIKRREEEIRLKVIPEWNETEKRALIQVIFGVENQKL
                     NPLLTLFHSTKTTISWFVLTALGLLYIILGKIPAEVAGPLGIAQIAGQAAQLGFLNLL
                     MFSAVVSVSLALFNILPIPVLDGGYLLLFLYEKIRGKPLEPEKIGWVYFIGIILIFSL
                     AIFATYQDIMRMVIGK"
     gene            complement(12959..14092)
                     /locus_tag="PK016_00530"
     CDS             complement(12959..14092)
                     /locus_tag="PK016_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01596.1"
                     /translation="MKVAILGSTGYLGEQILEVLARRKDFEIILISGYSKERRLREQA
                     QEFSVPYVFNPQGRKDFWKNSSTNLITDYDLLEEVIFQEVEGVFFAASGIEFLDLFWK
                     LMDTSHILWVASKEIIILAGEIKSHLLKERENLIPLDSEHNALWQMLKWVKREEISWV
                     YLTASGGAFYEWEGRWDDITPEMALAHPNWKMGAKITVDSANLVNKGLEVIEASSLFG
                     FSVEEVEVVVQRESFIHALIGLRDGFSLALLSPPDMRSVILNAISLKKREANPFQSLD
                     FSRIKSLNLSYPVPEKFSGFYLAREAGKRGKGYPALFCGADEALVRAFLSREICFGEI
                     SLLLERILEQKINPPRSLEEVKAIYRRGYQSAQTLILERKSIQ"
     gene            complement(14095..14904)
                     /locus_tag="PK016_00535"
     CDS             complement(14095..14904)
                     /locus_tag="PK016_00535"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HXL01597.1"
                     /translation="MKATTQSSDLKKRAWSVIWALPVFLFLILFSPQTLILVFFFLSL
                     GSLREYGQLVYERGEWTPWLWFSLASLVLLYLSLINIDYKIILFSGYILFLVLIGWGL
                     KKPEQIVEQVAFYLLGFLYCSLLPFFWVKMGIAYDRGSIILFALPVWTNDIFAYLVGR
                     KWGKHKIVPRISPGKSWEGLAGGIGMATLTGLILVTPPVSFSLFQGLLAGFSIALVSF
                     LGDIWESVLKRRAGVKNSGNFLPGHGGILDRFDSFFLVGPLVYLLRLSWGG"
     gene            complement(14901..15629)
                     /gene="uppS"
                     /locus_tag="PK016_00540"
     CDS             complement(14901..15629)
                     /gene="uppS"
                     /locus_tag="PK016_00540"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HXL01598.1"
                     /translation="MGKERERDNDRIGIILRHVAIIMDGNGRWAERQGLPRLEGHRKG
                     VEVIRNLVEETVKAGIPFLTLYAFSTENWKRPINEIQGLMRLFMESIDKYGKELEENR
                     VKVKFLGRKDGLPQDLTLQINDLEKSTLKGENLNLNLAINYGGRDEILRAVKNIYQTD
                     HSLLTSLSEENFSIYLDTGNQPDPDLLIRTGGEKRISNFLLWQIAYTELWFTDTLWPD
                     FTVEEYHQAFQDFTGRKRNFGGRV"
     gene            complement(15593..16159)
                     /gene="frr"
                     /locus_tag="PK016_00545"
     CDS             complement(15593..16159)
                     /gene="frr"
                     /locus_tag="PK016_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HXL01599.1"
                     /translation="MNENLKSVVKDVEKRMKQAINVAREELSHVRTGRASPALVEDLE
                     IDYYGTIMKLKQIASITTPDARTIIIQPWDKNALSLIEKVIWKSDLGFNPQVEGGAIR
                     IIIPPLTEERRREIAKMAKKWIEEAKVAIRNLRREANEEIKKMEKGGEISEDDSERAQ
                     AEVQKLTDEHINELDSLWEKKEKEIMTV"
     gene            complement(16152..16895)
                     /gene="pyrH"
                     /locus_tag="PK016_00550"
     CDS             complement(16152..16895)
                     /gene="pyrH"
                     /locus_tag="PK016_00550"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HXL01600.1"
                     /translation="MSDQQSSPLRFKRVLIKLSGEALMGSLPFGIDIQVLDYITKEVE
                     KVKNAGAEICIVVGGGNIFRGRTAGDKGISRVTADYMGMLATVINALALQDFLENEGI
                     PTRVQTAIEMRAVAEPYIRRKAIRHLEKGRIVIFAGGTGNPYFTTDTAAALRAAEVRA
                     EALLKATKVDGIYEADPLMEKNAYKFDHLDYLEVLNRGLKIMDSTAISLCMENNIPII
                     VFNVNKADNLVKIVTGHKIGTLVGREKNE"
     gene            complement(16888..17490)
                     /gene="tsf"
                     /locus_tag="PK016_00555"
     CDS             complement(16888..17490)
                     /gene="tsf"
                     /locus_tag="PK016_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HXL01601.1"
                     /translation="MSATAKEVFELRNLTGAGVMDCKKALEEAGGDIEKACDILRRKG
                     LEAAAKKQGRVAQEGLIGAYVHTDGKIGVLVEVNCETDFVARTDEFRYLVKELTLQVA
                     AQAPRWVAPENVPEEIKKKEEEIYQEQLKDSNKPEEVKQKIVEGKMKKFFEENCLLEQ
                     PYIREPEKKVKDLLMETIAKLRENIVVRRFVRYKLGEDNE"
     gene            complement(17490..18362)
                     /gene="rpsB"
                     /locus_tag="PK016_00560"
     CDS             complement(17490..18362)
                     /gene="rpsB"
                     /locus_tag="PK016_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HXL01602.1"
                     /translation="MMVVTMKQLLEAGVHFGHQTRRWNPKMKPYIFAERNNIYIIDLQ
                     KTVELAEKAYNFVRDLAREGGTFLFVGTKKQAQEAIEQEAKRCGMFYVNQRWLGGMLT
                     NFSTIRQSIERLKKIEEMEEKGILERLPKKEVMQIKKRKTRLEKYLSGIRDMERLPDA
                     LFVVDPRRERNAVLEARKMDIPIIAIVDTNCDPDEIDYPVPGNDDAIRAIRLFSALVA
                     DAILEGKRLAGEGQEMIATEGIAIPEEVEEEKKETELVAQEEDASQILEELQDLGLEE
                     IEEEDTEEQEDDDN"
     gene            complement(18499..19878)
                     /gene="hslU"
                     /locus_tag="PK016_00565"
     CDS             complement(18499..19878)
                     /gene="hslU"
                     /locus_tag="PK016_00565"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HXL01603.1"
                     /translation="MPLIEESLTPEEIVEELNKYIIGQDEAKKCVAIALRNRIRRRML
                     PPDIAEEITPKNIIMIGPTGVGKTEIARRIAKLTNAPFLKVEATKFTEVGYVGKDVES
                     IVRDLTELAVRMEKNERIKSQEEKAREIVEEQILDYLLALNPSSLEYEDTVASESPRV
                     DVVWPRPLSSTREKMREKLKRGELEERFIEIETEESSIPLGIIAGGGMESLGIDFQDL
                     FSGVFPPRKKKRKTKVKEAREILFNQEVQKLIDMEEISSEAIRRVEETGIVFIDEIDK
                     VVSPGGGYGPDVSRGGVQRDLLPIVEGSTVLTKYGPVRTNHILFIAAGAFSQTKPSDL
                     LPELQGRFPIRVELSSLTEEDLYRILIEPFNSLTKQYKALLETEGVSIEFEKDALREI
                     AHISQELNQRMENIGARRLHTVVERLLQDVSFRAPYLKGQKVIINRDYVEKQLAGVLQ
                     DEDITRYIL"
     gene            complement(19894..20421)
                     /gene="hslV"
                     /locus_tag="PK016_00570"
     CDS             complement(19894..20421)
                     /gene="hslV"
                     /locus_tag="PK016_00570"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HXL01604.1"
                     /translation="MIKSTTVIAVLRNGKGAMACDGQVTMNETILKGGARKIRRIYQD
                     KVLAGFAGSGADGLTLLERLEEKLNYYSGNLPRAAIELAKSWRTDKVLRQLDALLLAM
                     DKEHILIISGRGDVLEPDEPIAAIGSGGNFALAAAKALWRETDKEAEEIARLSLQIAS
                     EICIYTNSNVTIETV"
     gene            complement(20414..21337)
                     /locus_tag="PK016_00575"
     CDS             complement(20414..21337)
                     /locus_tag="PK016_00575"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HXL01605.1"
                     /translation="MSSPANEKNIYIFLEYLQKERRYSPYTVDNYGRTLRQFSQFLEE
                     RDLTLVQAKTQDLADFLAYLKTHHHLKRISQANRISALRTFYRFLRRRGIVDCDPTDG
                     LGKLNKEKKLPNFLTVGEVEFLLNHLKQKAREKQDFPSLRNWAFFELLYSSGLRVGEI
                     TQLKIKNLDLASKMVKVKGKGGKERIVPFNESTQEALREYLSHCPFSREEVFLSQRGT
                     PLTSRGARKILEAITKEAGLGGKRISPHVFRHSFATHLLGGGAELRMVQEALGHSSLS
                     TTQIYTHLNWDKMKRVYQETHPHAKKVKNDD"
     gene            complement(21318..22649)
                     /gene="der"
                     /locus_tag="PK016_00580"
     CDS             complement(21318..22649)
                     /gene="der"
                     /locus_tag="PK016_00580"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HXL01606.1"
                     /translation="MKEKIPLVAIIGKTNVGKSTLFNHLSRRRIAIVDPQPGVTRDFL
                     ENIVDIEGKKIKLVDTGGLEILIDSQNEMQKKVEEKTWEVIGKADLLLWLLDGRENLT
                     SIDWELASHLRKIKNPVIMVINKREGMTSEALPAEFFSLGFESVMQISAAHGDGLKEL
                     RKTITRIVEGNQEIYFDRHRISVAIVGKPNVGKSTLYNSLLGKERSIVSDVPGTTRDA
                     LLSTLDTSFGYYELMDTPGLITRPKIKERIDFYAALRSEESLQDAIIALLVIDPIQGI
                     TRQDKRIAQKIKEEKKGCLVFINKTDLVLNHHSRGQAKRLIEEIRQDLGFLNYASFLC
                     GCALSPSVSSDIFSVLAGIATRYYQRIKTSFLNEKIKEKINEESWKVGGRTFRTYYMH
                     QKDIAPPRFYLSVNVSATKREEELLANRIEKLIRKEGDWEGVPISIELSGE"
     gene            complement(22582..23514)
                     /gene="ispH"
                     /locus_tag="PK016_00585"
     CDS             complement(22582..23514)
                     /gene="ispH"
                     /locus_tag="PK016_00585"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HXL01607.1"
                     /translation="MEVQVANRIGFCPGVRRAFDLALRALSQGKPMYFLGELVHNSQA
                     VKMLEEEGGIIVTDPEDIPPFSWVVTRSHGLEKETKEFLENRQIKIIDTTCPRVKKVQ
                     ILAQELEEKGYSLIILGSPEHPEVMALLSFISQKALILENREEWGELNKFSPREKIAV
                     VEQTTFPREIKEEFTQEIKGLFPHLNLEVFDTLCAETEMRQRDLIESIKLHRINKVVV
                     VGGKNSSNTRALFFTARRWGIESIWVERAEELENEYGGKEDRILVVSGTSTPHFSVED
                     VVKKLKVREASFYERENTLGSYHRKNKCGEIHPI"
     gene            complement(23516..24172)
                     /gene="cmk"
                     /locus_tag="PK016_00590"
     CDS             complement(23516..24172)
                     /gene="cmk"
                     /locus_tag="PK016_00590"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012057825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HXL01608.1"
                     /translation="MKEHIAIDGPAGAGKSTVAQKLAWCLKYLYLDTGALYRAVTLYF
                     WENHIPFEDSENLSSLLTQIKIKVSWEDKFRVFLDDREVTEDIRQPEVDALVSPVAKI
                     KEVRDYLRSVQREIALSRPSVTEGRDIGTVILPDADLKVFLTARPEIRAYRRWKERKN
                     EGNNIDYKMVLKNVLMRDFIDSTRDDAPLQKAEDAIEIDTSFLSIDEVLEKLYKLVEN
                     "
     gene            complement(24169..25491)
                     /gene="aroA"
                     /locus_tag="PK016_00595"
     CDS             complement(24169..25491)
                     /gene="aroA"
                     /locus_tag="PK016_00595"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HXL01609.1"
                     /translation="MREVLVHPFPQKVEGEIRAPRDKSLSHRAAMVGALSQGVTVIDG
                     FSFCADCLSTLASLGLWGVEIIKEEEKERITVKSEGLGKLKEPEDVIDAGNSGTTMRL
                     LAGIATGIEGLTIFTGDNSLRRRPMKRIMEPLRLTGVDVGGRNKDSFPPFFVRGKNRV
                     KAFSYSLPVPSAQVKSALIFAALRGEGVSFIEEPLLSRDHTENLLRYSGVKIERKGNV
                     IEVEPPSSISASYLSLPGDLSSASFLIALATLLPQSHILIKDVGMNPTRSGFLACLRE
                     MGGNFKIIGEKEEFGERRGDIEVESSSLRGIKIEKDLVPLVIDEIPVIAVLAAKAKGT
                     TIISGAEELRVKETDRLKAIAQGLRKLGVEVEEMKDGLLIQGPCVFQGGRVDSYGDHR
                     IAMSLVIAGIVGEEDLIVEDIDCVRISYPEFFEDLGKIGYGEWEIRER"
     gene            complement(25488..25871)
                     /gene="aroH"
                     /locus_tag="PK016_00600"
     CDS             complement(25488..25871)
                     /gene="aroH"
                     /locus_tag="PK016_00600"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HXL01610.1"
                     /translation="MEKKVRGIRGATTVEQDDKEEIKKATKELFDLLLRENDIFPSEI
                     VGVFITATPDLHSTFPAEVIREREEFRYVPLLCTQEMKVEGALEKCIRLLVLAYTDKN
                     QTDIHHLYLGKASSLREDLNRGKNL"
     gene            complement(25873..26394)
                     /locus_tag="PK016_00605"
     CDS             complement(25873..26394)
                     /locus_tag="PK016_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HXL01611.1"
                     /translation="MADKSGLLQVYFGEGKGKTTAALGALLRAVGQGMKVLLVQFFKR
                     KFTGELASLQLLPGVNVYQFGSGKFITGEVFPEDRREFFLGWQMVREALEKGEYDVVI
                     LDELPYAFYYHLLTWEEFRKALEQKDPGVEVIITGREVPEELIAIADLVSEIKLVKHP
                     WDKGISDRRGFEY"
     gene            complement(26384..27115)
                     /locus_tag="PK016_00610"
     CDS             complement(26384..27115)
                     /locus_tag="PK016_00610"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HXL01612.1"
                     /translation="MERLNRFLAQAGIASRRKCDFLIKEGKVTINDRVVTNPGERVSG
                     SEIIKVENKIVGKKEELVYIELYKPRDYLTTLCDPRGRRTVVELLSGITARIFPVGRL
                     DSESEGLLLFTNDGALSYFLTHPRFEVEKRYLVYVSGNIGEKKLSFLKKGIEVKGIFY
                     HIQQGEIKETHSNWSMVEVTISEGKKHEIRIMFDYLRHPVLRLIRVGMGPLLLDKDLL
                     PGEWRYLSDEEIQLLQKYREEKFGR"
     gene            complement(27099..27662)
                     /gene="scpB"
                     /locus_tag="PK016_00615"
     CDS             complement(27099..27662)
                     /gene="scpB"
                     /locus_tag="PK016_00615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HXL01613.1"
                     /translation="MAEYISEKPVLQLKNLIEALLFSSSHPVSCSEISKLTETDKETV
                     IKTLQELRNEYEKREGALVIREINGRWQMIVKPEYGQELKEKIKVNSTKTISRKALET
                     LAVISLYQPLTKTQIDLKRGVDSAQTIRTLLERGLITVAGQGDLPGKPFLYKTTDKFL
                     EIFGIESEEELERLKNLFQEKKNGEIK"
     gene            complement(27610..28215)
                     /locus_tag="PK016_00620"
     CDS             complement(27610..28215)
                     /locus_tag="PK016_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01614.1"
                     /translation="MILNPESQWKKLLQETPFPQCIEVWEEVLAFLESQLDEALGKGE
                     EENEEANLSQEKEEAFLLFTQLLFNLEEREQSTLKAPEKRWEAFQNREVKELEKLTLA
                     ELLSLYELYLTPETPPDVSLDSQFQKLLEERKITIKKMIEEKELWEMDEFFHDCLDRK
                     TTIATFLILLDLVFRKKLQMKRGENGRIYFGKTCPPVEKSD"
     gene            complement(28199..29200)
                     /gene="trpS"
                     /locus_tag="PK016_00625"
     CDS             complement(28199..29200)
                     /gene="trpS"
                     /locus_tag="PK016_00625"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HXL01615.1"
                     /translation="MKDKETIFSGMRPTGKMHIGHYFGALKNWVELQEKYHCIFGITD
                     WHALTTSFEDTAQLENNIIEMAADWLAVGIDPEKSILILQSWVPEHAELHLLFSMITP
                     VGWLERNPVLKEQLQDMGLKEAVGYGHLGYPVLMASDILIYKAVKVPVGEDQVPHIEI
                     TREIARRFNYLYGEVFPEPQALLTEAPRILGIDGKKMSKSLNNYIGLSESPEEVKKKV
                     RSMFTDPEKIRLSDSGHPERCNVFSLHRLLANPRLTEIEKDCREGKWGCVSCKEELAE
                     LMTRFLEKVVPHREKYQREKQLVKEILWEGSKKARQLARATLEEAKEAMKLNYDFKS"
     gene            complement(29190..31781)
                     /locus_tag="PK016_00630"
     CDS             complement(29190..31781)
                     /locus_tag="PK016_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXL01616.1"
                     /translation="MQIVVSHEGTDFDALASMFAVTRLFPGTQMVIWGTVNRNVRHFL
                     SLYGNFFPILKEKEVDWEKVNKIYVVDTAYWERLNKAGELIRDGKVEVVIFDHHSEGE
                     KREEAAIIKEYGACTTILVEKMMEQKIALSPLEATLLALGIYEDTGSFTFPTTQAPDL
                     ESASFLLRQGAKVSFVNRFTGIQLTPEQRETLKKMINFLHAEEIEGIPVHFCRMRLTE
                     YVEGISLLVHKLMDLEEIELLFVLMETENKVYVIARSRLEEVDLAKILGKMGGGGHRS
                     AASLTVKGKKIEEVEEMIREALAASLSFTRAKEVMSYPVKVVSPSTTIGEALLLLMRF
                     GFSGLPVVDEDGKLVGMIARRDVEKAFRHGLQNAPVKSFMSSQLVTVDSEDSIFKVRN
                     LMVEKDIGRIPVVKGDTLLGIITRSDLLRVLHQRENSVVQELTRLNLAEKLYLHFSPL
                     DLQLLHFIGDESKRAGVKAYLVGGVVRDIILGFPGYDLDIVVEGDAIELAQTIVQKLG
                     GKVVPYPPFGTAILFLKDGRRVDFASARSEYYDYPGAPPHVEYSNLRKDLFRRDFTIN
                     AMAISIGTDDWGDLFDFFGGLRDLENKILRVLHPLSFVEDPARAIRAVRFEQKYGFEI
                     EPFTMSLLKQTAREELLNKIKPDRLKEEINLILRLSDFYRSLERLYQLDMFPYIFPGS
                     TWRREFEPIYQRLVKLLDQFSSPSLDYFLAKLSPLWGEMDPAYLEELKNKLALSKVCV
                     RKIKVYLDNKEELEIKLLDGTLPNSQVYLLLRPLSDEFLLLLEAKWGEDSLPRKRVEQ
                     YLKEWRKIVPCLTGEDLRRKGIPAGPVYAKILEELKLARIDGLVKSKEEEEILVEKIW
                     RNETNER"
     gene            complement(31765..32592)
                     /locus_tag="PK016_00635"
     CDS             complement(31765..32592)
                     /locus_tag="PK016_00635"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HXL01617.1"
                     /translation="MVREQALLSRYYPEKILLRFQEIISSFSEKPQLAIVAGSGWEET
                     VKGELIGEFSYPYLGIPFFTEVEGHAGKLCLLKKNDFTFLLFSGRLHLYQGYSFFEIT
                     LPVVFSYLAGVEAIILTNAAGSLSFSLPPGSVVIITDQVDFTFYPDPILLPPPFFDEK
                     IQEIFKKAGRKGSITLNQGVYTGVLGPSFETPAEIRFLSRSGDMVGMSTVKESKLASF
                     LGLKVGGISLVTNWGAGMSGSLIHHQEVLEIAVSLQPLLQRLFSSFLEEWSKNADRS"
     gene            complement(32537..33718)
                     /locus_tag="PK016_00640"
     CDS             complement(32537..33718)
                     /locus_tag="PK016_00640"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HXL01618.1"
                     /translation="MRIFIALLDGVGIGELPDASFYGDEGSHTLRNTAQAVGGLSLPT
                     LERLGLGWIDDIPGVKKVEIPLALSGKMNEKSKGKDTTTGHWEIAGIILEKSFPVYPR
                     GFPPSIIEELERAWGRKILGNKPASGTVIIEELGEEHLRTGYPIVYTSADSVLQIAAH
                     EEVIPLPELYEMCQKAREIMRGEHAVARIIARPFVGRPGQFKRTANRRDFSLPPPKDT
                     ILDVLFRQGSENIGVGKIQDIFAGRGLNENIKIADNKEGFQILRELQKSKKGDLVWVN
                     FNDFDTVFGHRNNPRGFAQALEAWDKDLSSFLPSLREDEVLFITADHGCDPTTPSTDH
                     SREYVPLLVFNPSNSQGVSLGIRKTFSDIAATIAQMMEVEWNGPGTSFAFQILSGKNS
                     P"
     gene            complement(33719..34276)
                     /locus_tag="PK016_00645"
     CDS             complement(33719..34276)
                     /locus_tag="PK016_00645"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXL01619.1"
                     /translation="MFFSYSENENDFSLPQEIKKEVLLEAGIFKVIKNTYQGGMERVF
                     IAHPGAVTIIATTPDDKLLLIKQFRAPAKEWLWEIPAGTLEPGEEPLFCAQRELEEET
                     GFSSNNWQHLFPLTLAPGYSSEIIHFFRAKDVSPVTEARAGDEDELIYLREISKKEAQ
                     KMLDRQEIKDAKTRIGIYEWMREER"
     gene            complement(34279..34938)
                     /locus_tag="PK016_00650"
     CDS             complement(34279..34938)
                     /locus_tag="PK016_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HXL01620.1"
                     /translation="MLVKDRMNKEVVTVPPSTTVLEAEKIMKDKNIRRLPVVEKGELV
                     GIVTYNDLLEASPSKATTLSRFEITYLLSKMSVAEIMTREVIYTKPDTPIEEAALIMN
                     KNQIGGLPVVEAGRVVGIITESDIFEILVETLGVREGGTRLTLELPQRPGVLHEVTGV
                     IREYGVNILSLATFYQDQKPDYRYVVMRLATREPEKIIEELNKNSDVNVCHVWITPEE
                     E"
     gene            complement(34913..35491)
                     /locus_tag="PK016_00655"
     CDS             complement(34913..35491)
                     /locus_tag="PK016_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HXL01621.1"
                     /translation="MHHEILIGGFGGQGILFLGRVLATAGMIEGYEVSWFPSYGPEMR
                     GGTASCTVIISEEEIGATVTDHPDICIVMNEPSFRRFSPLVRRGGLLVVNSSLIRENC
                     SRRDIEILQVPANELAGKEVGVAETTNMVILGALIARQPVVSFSAVKKALEKILVGKK
                     ANLLSVNFKALEVGKSFEEKGGVVNVSERPDE"
     gene            complement(35481..36236)
                     /locus_tag="PK016_00660"
     CDS             complement(35481..36236)
                     /locus_tag="PK016_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXL01622.1"
                     /translation="MKTIFERPKTLINKPFTYCPGCHHGLAQRLIAEVIDELSIAEET
                     IGVGPVGCSVYIYEFFDLDFVMGAHGRAPAVATGIKRSLPDRVVFSYQGDGDIAAIGT
                     AEIVHCANRGELITSFFINNAVFGMTGGQMAPTTLPGQKTTTSPLGRNTEAQGFPFKL
                     GEMLAQTDGSAYIARVALTNPRLISQAKRCIKKAFLTQIEKRGFALVEILSPCPTNWG
                     MTPQEAVRWVEEKMIPYFPLGEIKVKEDSSRAS"
     gene            complement(36233..37300)
                     /locus_tag="PK016_00665"
     CDS             complement(36233..37300)
                     /locus_tag="PK016_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HXL01623.1"
                     /translation="MERILMKGNEALAEAAVQAGCRFYFGYPITPQTEISEYLSRRLP
                     EVGGVFLQAESEIAAINMVYGASSTGARVMTSSSGPGISLKQEGISYLASAELPCVVV
                     NMSRGGPGLGNIQPAQSDYFQATRGGGHGDYRTLVLAPSSVQELVSLTHRAFYLADKY
                     RNPVKILGDGMLGQMMEPVTWEGIELPSLPPKDWAVKGKGEKERKFILCMELDPQELE
                     NFNRKLEKKYQQMAEEDTDWEVFGEEDNPDLLLVGFGTVARILKTVVREGRKKGWRLS
                     LLRPITLFPFPYRVVQEMAEKAGRVLVVEMNLGQMVEDVRLGVEGRAPVNFYGRVGGM
                     IPTVDEILQKIANILEGEGQK"
     gene            complement(37305..37517)
                     /locus_tag="PK016_00670"
     CDS             complement(37305..37517)
                     /locus_tag="PK016_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXL01624.1"
                     /translation="MAKAKVNINQEFCKGCGLCVNICPKKILTIEDNFNSKGYYPATM
                     IDEEKCTACGFCAQVCPDVAISVYRE"
     gene            complement(37530..38234)
                     /locus_tag="PK016_00675"
     CDS             complement(37530..38234)
                     /locus_tag="PK016_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01625.1"
                     /translation="MQILKNSSFLSQEMEEKEIFILAGPNGVGKTEIALNLTSFFQSL
                     GMGSYLLDLDFTKGDFTARSDRFLSQVSILPQGKGRYADTPLWDKEIPEILSQIDKNH
                     RLIVDLGGDLKGMKSWRSLQSFWRNKEVHLTLVTNFCRPFSEEENYSDFIEKARTVLN
                     LDVHSVISNTHLIEYTDEDILKMGWQKTGDFSQKESLPIFFVSIWESLVRNLSWNIFD
                     SAVVGIQRFLYLPWME"
     gene            complement(38221..39891)
                     /locus_tag="PK016_00680"
     CDS             complement(38221..39891)
                     /locus_tag="PK016_00680"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01626.1"
                     /translation="MLRTLIIKNFVLVDYQEINFAPGFNVVTGETGAGKSLLINAFSF
                     LLGARPREEWIRKGSDFAQVEGWFDISFSPLLQEKLKEKGWSEEIKGGMVLSREVNRE
                     GKSKAHINGRSVTLAALKEVTSHLVDIYGQGENNRFLTPENKLAILDSFLDEEGKLDR
                     ENYLHLKEERNKIKEKIQELKRGQEVEIEEIREMLEEMEKMGLTLEKIEEVEEQFQRC
                     VNSQRYQEIAEEIIYLISGAEEKEGFLSSLRRVRRNWEGKDELALFPPSVEETLKNIE
                     LELQEVLYQVEKVRNSFDFSPLKVEELEKEVAEIERWKRKYHCLTTKDLISFIEEKKI
                     KLSQLYRELEELEEWERELEIKEKNLMEAKEKLFHHREKAAKVFKEKLEKELKELSFS
                     KVALEIELEKKEEDDEVSFLISLNPGEPLFPVEQVASGGELSRIILAIKSLTREVRGI
                     PILVFDEIDQGVGGQTAFWVGDKLRRIATGHQVISITHLPQIACFAHHHLKVEKRGDE
                     ESTWVEVSSLSEEERIKELARMMGDGGQDNSALQYAETLMKRAQNANT"
     gene            complement(39885..40742)
                     /locus_tag="PK016_00685"
     CDS             complement(39885..40742)
                     /locus_tag="PK016_00685"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HXL01627.1"
                     /translation="MDKVLSTAHIFIKKKIQKVRDKARELDNFLRSQGINTLIVEGET
                     QIEEKPEVVFVLGGDGTFLSASWLYSPSGVPLLGIDMGGLGFLAEASVENMFQAAEEV
                     IRGRFEVEKRMMAQCHISHRDGKSDSDFALNDVVVYRGPFAQMIRLSTYIDGEFLASF
                     PADGLIVATPTGSSAYSLSAGGPVLLPTLELLVVTPICAHTLYARSIVVDPSSTIEIV
                     LESEKEGTMVTLDGQRGYLIQKEEHITVARSNFSAHLIRVGPKRPFYSLLRSKLSWGM
                     DIRRQVEEC"
     gene            complement(40727..41245)
                     /locus_tag="PK016_00690"
     CDS             complement(40727..41245)
                     /locus_tag="PK016_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01628.1"
                     /translation="MNFNPWVWGMIFSFSVAVVITMFVSSAWRRHRRKKIFSRAQVVE
                     KEAASLLENSGFKLVEEQPEREFFIQVNGKKIPCKARADYLVRRGKDLYVAEVKTGEY
                     LHFDHPRVRRQLLEYYLVYRPKAIILVNGESDELYRVTFPFSNASIWRWMVILSILFF
                     VLGLIIGRWIKF"
     gene            complement(41250..42065)
                     /locus_tag="PK016_00695"
     CDS             complement(41250..42065)
                     /locus_tag="PK016_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014901980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HXL01629.1"
                     /translation="MKKEKIRLDELLVKSGLCDSREKAQRIIGSGKVKVEGLGQLLKP
                     STLVEKGAFIQIEEPPRFVSRGGEKLEKAITEFQINPQGLVFADIGASTGGFTDCLIQ
                     RGASKVYAIDVGYGQLHEKLRHHPQVVVKEKVNARYLDLSFLGEKVDGVTIDVSFISL
                     RLIFSPAASILKDDGLLIALIKPQFEAGRQEVKKGVVKDKKVHERVLEETLTQAQEAG
                     FSFQGLTFSPIRGAKGNIEFLGYWVKRSSDLTNWSLRSIINQVVEEAHQSKGK"
     gene            complement(42069..42956)
                     /locus_tag="PK016_00700"
     CDS             complement(42069..42956)
                     /locus_tag="PK016_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HXL01630.1"
                     /translation="MKIEDFLQENAHLVDEYLERYFSPLSVTSSLVSPIIYGVKGGKK
                     IRASLALATGLSYGIEKEHLLPVASAIEMVHAFSLVHDDLPCMDNDDYRRGKPSMHRA
                     FGEAMAVLAGDALLIEGLRLIVGNEQLVEFFGKEKVLDMVNVILKALGIEGMVGGQVL
                     DLEGEGKEVSEEKVWEIYRKKTASFLTASILCGAIAGSATSQEKEVLEKFGMLLGECF
                     QVKDDILDATQEKEILGKTPGKDEIQDKATLVKIKGVKESEKIMKNKEEEAIHLLLSI
                     PRSFPLLLEIARFVIERKY"
     gene            complement(42953..>43228)
                     /gene="nusB"
                     /locus_tag="PK016_00705"
     CDS             complement(42953..>43228)
                     /gene="nusB"
                     /locus_tag="PK016_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HXL01631.1"
                     /translation="KKKEIDEVLSQASEEWPLYRMANVDRNILRLAIFEIVFLPDVPV
                     SVAINEAVELGKKYGTEESGKFVNGVLGKLAKNLKERENYLPKREKT"
BASE COUNT        12560 a         9742 c         7217 g        13709 t
ORIGIN      
        1 cttggcgcgc ctggagggaa ttgaaccccc aacctacgga ttagaagtcc gttgctctat
       61 cctattgagc tacaggcgca acctgcaaaa attttataac cttttttctt ttccgtcaac
      121 ttttcagcat ttacaaatat ggagaagtat ttctcgtaaa gctctgattt tttcttctga
      181 atttcctgag aaaaagagag aaatcacttt ccttcccgct cttttaagcg cttctcggtc
      241 tcccaaagcc tgagcttttt tcaatgttcc aaaggtaaaa gaagaaacat cacttcccgc
      301 ttcttcggga ataggtatat ctagtgaaga ttcacttact agctgtaaga atagccccct
      361 tcctccatct cctttatgta attggccagt agaatgtaaa aagcggggtc cgtagccaaa
      421 agttactgcc ttcttagtcc tatctcgaag aagggaggca agcaaaatta gctctttttc
      481 tattggagga ttaacaggaa gataagcgtg aatagaaata tagctacttg actccacaaa
      541 tcctaaaaac tgttttagaa cctcttctaa ggaggaagca gaaaaatcac aaaatacatt
      601 gaatcctttt tctatcaaga tgggtttttc ttcttcccag ctaccttctt tatactttct
      661 catcatttcc tgagtgaatt ttttggtaga ttctacattg ggttggtcaa aaggatttat
      721 ttttagccag taaccggata aagcagtagc aaattcccag ataaatattt gttcccctaa
      781 atcgtaatta tcagtgatag gtatttttat aaccgggtgt tcagcgtctt ttaactcctc
      841 aagtaaatta tcgagagagt catttttttc tccctctata taaatgaaca agcggtcttt
      901 tccatatact tctggtttac caggttgttt gtcgataact ggtaatattc cttttccctc
      961 tttacctgtg ctttcagcaa tcaattgttc taaccagtca ccaaaacttt cccatcgagg
     1021 agaagagaag aaaaaagtta gtttatctct acctttttgg gctaaaactc ccaaagccgt
     1081 tcccaggata gcaccttcat tattttcaag aagggtggag ggtgaacatt tttgggaagc
     1141 aagggtagct ttttcaagaa gaagagataa atctattcct atgagggtag caggcacaag
     1201 accaaagaaa gaaaaagcag aataacggcc tccgatatcg ggagaattca aaaatacttc
     1261 acgaaatcca tatcgttctc ctaactgaac gagaggacta ccagggtcgg ttatggcaat
     1321 aaaatgtctt cctgtttctt tcccgagtct ttcctgtacc agattataaa aaaacttgaa
     1381 aaaggagaga gtttccaagg tatttcctga tttggtagag acaatataga gggttttacc
     1441 agggtccaaa gactgagcgt agtagtttac catctcaggg tcagtactat caagaacatg
     1501 gagattcaaa aatccctttt tgcttcccca cactttttta aatagatcag gagccaggct
     1561 cgatcctccc attcccagaa gaagagcctc ctgaaaacct tcttttatca gctcatcagt
     1621 catattttct aatcgagcta catttttttg catctcttga aaaacattta accaacccaa
     1681 acgattaaca atttcctgag gggatggttt ccagagagta taatcttttt cccacaggcg
     1741 aggaattacc ctttcttttt ctaagataat aaaggatgat tctatctcct tttgatattc
     1801 tccccaaaat gtttccattt cacttcctcc tttcaaaatt tctttcaaga aacacttaat
     1861 atcaaggtga taataaaagg caagggttta tttgaatatc aacaccttct tttccaaaaa
     1921 ccaaatgagc tctcataaag ggtttttctt cactcccttc cacaatgaag cgagcctcgc
     1981 tgcatccttc agctatgggg ttagacactc ctttgttttt gagtaataaa gttcctcctt
     2041 gggaatagat ttctaaggga ttagaggata aagtcatggt tagttttcta ccgttagaat
     2101 aagattgaag attatcgcta tacctcacta actggaagag ggtatttata gacccgagaa
     2161 gattttcttc ttcctctatt ttttgggata aggaattaaa aagttttccc cataaaataa
     2221 ctaaactact aactgccacc actaaaagta gaagaataac aaaacttatt aaggcttcga
     2281 tcaagaggaa gccttcatca tttttcccga agtgtttgca aagaaagaat ttcctcacca
     2341 tttttgtcct ccaccattac tttaagctca taaatttcag gggaaaaatc ccctacctga
     2401 ctttctatca caaactcttc tttttcttct tttcctgagg cagggacttc gtcatttttt
     2461 aattcttcca tagtgtttac agcgtctata agagctttaa tcttaatagt agagcttttc
     2521 tgagtctttc caataaaatt tatttcctgt ccaacgacca gtattaaaaa agaaaagata
     2581 cacaaagcaa taagaatacc tattagagta aagccttcat catttttcac tgatgcgaac
     2641 tctcccggta actggagtaa taataagatg caatagtttt tcatttattc taatagtaac
     2701 cgttcctccc atagaagggg taccagtagg gtagaaaagg atagtatgtc gactgaagtt
     2761 agtagtacag aggtctaccc ctttaggaaa attaactctt ttagaagtgc aaccttgata
     2821 ggtgaaaaaa tcctgaacag ggtcaaagat aatctgggta gttactccag ttcttaatgc
     2881 ctctatctta gctagacgta actcgctagc tattctctga gcagtaagtt ttcccagaaa
     2941 ggaagcttgc cagccaagta agcagggaaa agaaaaggct gaaatcacag aaacgataga
     3001 aagcactatt aagagttcaa gaacagtaaa acctgatgag aaattttccc ggctcgcttt
     3061 catttccttc actgtttctc ttgtttatga aacataatac atggtttttt cagatttttc
     3121 cataagtaat tctgcatttt tatgagattt tagtgtttta tttatctata tctcgatatt
     3181 attgggataa ccccctctcg tcaatcgcga gcctcgcctt aacagtccgt agcatagaat
     3241 aataaagcta cagaagagaa agggcccttt ataattccat agaatataca agacttaaaa
     3301 tagagatatt aaaatctact gggaaaaaat ccctcctcag tgcgtggcaa tcccgagttt
     3361 ttaacttcaa tataacttgc ttttaaaatt tgagcaattt aacaggttct gcagaatact
     3421 ataattgaat tgtaattgta gagtagaaga agtaaaattt gtgaaatgag gtgagtaaaa
     3481 taacgaatga atggttatta atagtattta taagttgcgt tttattttat agctacgccg
     3541 gtatttatat ctcgcgtcag actggtgctt caatacctcg atggcccagc cttataaaaa
     3601 gcagagatta cactttttat atcatttata tctgttccat agctcttatt attaatacta
     3661 tcatttcttt gatgggagag tactcattat tctatttatt tttaatcggt ttagtattga
     3721 tgttctttgg tatttactta aatattcttg ctcggaaaga attaggtcgg tattggtcac
     3781 cactttcttc ttctgataat agtgaaaaac ttattaagtc aggggtttac gctcgcgtta
     3841 gacatcctat ttacctttcc aatctgatct tctttttagg agtaacctta attgccggaa
     3901 gttggtatag ttggatacta tttatcttat ttcttggggg attaaggaaa agagtgaaaa
     3961 aagaggaaga agaactctac aaaaaattcg gtgaggagta taggatatac gcaaaagaaa
     4021 ctcctttaat attaccaagg ttaagaagat aaattcatat aaagaggtag agtagaggag
     4081 agagtattcc attttgtctt taagcagagg tgattaatat tatcccggtt actaaaatag
     4141 ttcaaaaacc tcttagtctt tgtacccttt cttcagttga gaaaggagtg atagaagagc
     4201 ttaattgctc aggagtaact atcaaaaagg gatctcgaat tgctattgca gttggtagta
     4261 gaggtatttc tcaaattcat gagatagtaa gagttgtagc ccagtgggtt aaagataaag
     4321 gaggttatcc tttcatcatt ccagctatgg gtagccatgg tggagctact ccagaaggac
     4381 aaagagagat tctagagagt tatggtataa aagaagaata cgtaggtgct cctatttatc
     4441 cttctatgga ggttgtagaa cttccgaaag gaaatttacc caatagagtg tacatgagca
     4501 aagtagctta tgaggctgat ggaaccgtta ttattaaccg agttaaacct cacactgatt
     4561 ttcatggtcg aactgaaagc ggacttctta aaatgtgtgt tattggttta ggcaaacatc
     4621 aagaagcttt agaaatgcat cgctttggag ccagaggact taaagaatta attcctgaaa
     4681 ctgccaaaca gattataaaa tatggaaaca ttgttctggg tctggctata atcgaaaatt
     4741 cttttcatca aacaatgtta gtgaaggcac ttaaaccatt tgactttgaa gcagaagaag
     4801 aaaaactttt aaaattggcc tgggaaaata tgcctgttct tccagtagat aaaatagata
     4861 ttttggtagt agaccaaatg ggaaaagatg ttagtgggag tggaatggat accaatgtaa
     4921 ttggaagaat gaacattgac ggcgagccag aacctgaaaa accccatatt aaagtaatca
     4981 tagtcagaaa cttaactgat gaatctcatg gtaacgctac tggtatgggg ttagctgata
     5041 ttataactga gaatttttac cgaaagatag attttccatc tacttatgag aacatcttaa
     5101 ccagtacttt tttggaaaga ggaaagttac cactgatagc caaaaatgat cggcaagctt
     5161 tagaatgggc actcagggct agccgaatta ttgatccaga agaaattcga ttgattcgga
     5221 tcaaagatac tttgagctta aaccagatgt atgttagctc tagagttctt tcagagataa
     5281 aagaatgtaa tagcatagag gtaacagaag actttaaaga tttattaact ccggaggaag
     5341 aggagttaaa ggatttttaa caaataatga ataaaccagc ccttcctctt ttagttatag
     5401 ctgatgatgc tactggagca ctagatactg gagttcaatt ctctaagcag gggtttccca
     5461 ctttagtagt cccagataac cagcttcctt taaggaatat agaggcgaat cttcaggtat
     5521 tggtagtaga tactaatact agaaatgttt ctcccggaga agcttaccaa agagttaaag
     5581 aagtggttga attagctctt tctcggtttg aaatccagag cttttacaaa aaaactgatt
     5641 ctactcttag agggaacata ggaaaagaat ttgaagcttt gcttgacgtg actgggggaa
     5701 aagagctttt ctttattccg gcttttcctc gaatggggag aattactcga ggaggctatc
     5761 aattaatttg tggaaaagct gcttgccaaa gtgatttggc tcaagatatt ctgaatccag
     5821 ccaaagaagg ttatattcct gaagttattc agaaacaatc ttctctttca gtagaagtag
     5881 ttaatgagaa tagaagaaat gtttttttga aatataactc taaaagaaat agaaggatga
     5941 taatagtttt agatgccaaa aacgatcaag acttaaagga aattgggtta attttacaaa
     6001 gagagggtaa aatctttctt actgctgggt gcgctggttt cgctgagtat ttagctcaat
     6061 ttttggattt acaaatagct aaaagtagag tttttactcc tccctcccaa tggttggtaa
     6121 taggaggtag ccttcatcct ctttctctgg agcaattgga ttttgcatct caaaaaggta
     6181 tacctcaggt tattcttccc cctactttct tttttgagga taacatagag ggtttttttc
     6241 aaaagatctg ttattatttt caaagggacc ctggttttat catcagaacg gtaaaaagta
     6301 gaaaagattt agaagaatat cgagagttag ctcaaaaaga agggtgggag tgggaaaaag
     6361 ttccttccct tatttcccaa aaattgggaa atctggtaaa agagttgata aaaagaagtg
     6421 atgaaagaag aacgataata gttttcggag gggataccac aagaggagtc ctagaaagct
     6481 ttagctggaa aagaataaat cccggggaag aacttcttcc tggagtgccc ctttctcagg
     6541 taataacaga agaaggagaa gaactttatt taattaccaa agctggtgct tttggagaga
     6601 aagaaactct ggtgaagttg ataaatgttt tgtaggagga agatagtgtt tattggagtg
     6661 actatgggtg accccaacgg ggtaggcccg gagataattt tgaattcttt tcgaaaagga
     6721 gaaacaggaa aagactttgt ggtaattggt gattatcagg taatggattt ctgtagtcgc
     6781 ttgctaaagt atcaagttcc tttaaaaaaa atagtcaatc tttcccaatg ggaaaagggc
     6841 aggcttaaca ttcttgattt aggccttcta gaagaaaaag atatagagat agggaaggtg
     6901 tcttccaaag caggttttgc tgcccaaaaa tatgttgaac gcgcgacctt gatggcctta
     6961 gacggtaagg taagtgcttt ggtaactttg ccggtaaata aaaaagctat tcggctaact
     7021 gatcctactt ttactggtca tactgaattt attgctcaac tttgtggtca atccaattat
     7081 gctatgatgt taacttcaga aaaattgaaa gttgttcacg taaccactca tattccttta
     7141 acagaggtag ccaagtttat taaaaaaaat agagttgttg aagtgacaac tcttgccgga
     7201 gaagccatgc aaatgctatt aggtagaaag cccaaaattg caatttctgg tttcaaccct
     7261 catgccgggg aagatggagc ttttggaagg gaagagatag aggaaattat tccggcagta
     7321 gaagaagtaa gaagtaagaa ctgggaagta gcaggaccta tttctcctga tgttgtcttt
     7381 cttgaggctt atcgaggaaa gtatgatgtg gtaattgcta tgtatcatga tcaaggacac
     7441 attccggtaa agctccttga ttttgaagga ggagttaacg tcactttagg tctaaaaata
     7501 gttcgtactt cagtggacca tggaactgct tttgatattg catacaaagg ggtagctaat
     7561 attaagagct ttgttcaagc ttttgatcta gctgaaaaac tatctaaata gaactaaaat
     7621 aaaaacttta cataatatac attatgggaa ctaaaattat tatttaaacc cgagcgagag
     7681 aaagatggca aattttatcg gcggcttctt ttcgaaccac acattttatc attacttctc
     7741 cgtttttcgt tgtttttaga tcttctatat atccctggtg gtatattgct ccctgcacta
     7801 gtggaagctg ggaatagtta acctgcaggc acaagtgcct aacctcatgt tgtaaggaag
     7861 aagaaatttt ctctttaagc tctttcagtc cccttagctc ctgtgccgat acaaagacat
     7921 aggtataatt ttcttttata tctcgttcta aaattaaggg tgagcaaaga agatcaattt
     7981 tgttaaagac taaaattcga ggagaattat ttatttctag ctcttctaag atttgattta
     8041 tgacttggaa atggagatag taatcttcat cagaaatgtc tatcacttcc agtattaagt
     8101 cagcatcttt aatttcttct aaagtagccc gaaaagcatc ttttatagta ggaggcattt
     8161 ctcggataaa tcctacagta tcggtaaaaa gcgcttctcc tattccaggg ataaaaagtt
     8221 ttcttgtggt agggtccaaa gtggcaaaca tcttcttttc tacataaacc tgagagccag
     8281 ttaaagcatt cagtaaggtg gattttccag cgttggtata acccactaaa gcaacattgg
     8341 gaattttcct ttctcgacgt aagttttttt gtacctctct ttgcctttct atttttctta
     8401 gctcctgatt taagttagca atacgatctt taatttttct tctctctact tcaatttttt
     8461 gttctcccgg acctcgagta cctatacctc ctcccgtctg ggataggtct ctacccttcc
     8521 cggttaaacg gctaagctgg taatggagtt gagctaactc tactttgatt ttcccttgag
     8581 cagtacgagc cctctgggca aatatttcgt gaattaaagc attcttggta tagacaggaa
     8641 cctgccagaa tttttccaga ttcctctgtt gagaaggagt tatttctacg ttaaacaaaa
     8701 taaaattaat ctccgggtta tcctcaatta aacttttaac ttcctctact tttcctttcc
     8761 ccaaataata cttataaaaa gggtttttca ctcgagattc tactactgat aaaattttcc
     8821 agcctgcact ccaagcaact tcctcggttt cttttctttc tccttcccga gaagaaaaag
     8881 aactatcatt tccccgatcc acaagaatca ggattcccct tttttcctcc aacttttatc
     8941 tattacctcc cttgtaaaag ttcttaataa tatcccggta cattttcatc ctttctctca
     9001 atcctttaac caatcctaat ttttcttctt tcattacttg agtaagcccg ggaagcttaa
     9061 ctaactttac tctcactcca ttttttcgaa tatagcgatt gatagctact tctactccat
     9121 aacgggaaac ttctatatca ggaatattcc ttagaatgtc gatccttata gctctctggc
     9181 cagaaagaaa aggggcaatt ttttgagcaa agtcggtagc aaaacgacca tcttcaaaga
     9241 taccaatggt aacgtcagct tcctcatcta taactggctt aactaaatct attacatgtt
     9301 gaggagtaag ccctattaag tcagcatcca ctaaaagaac tacgttagaa gtggtattca
     9361 tcaacccttg ataaagagct ccacctttac ctacgttata caggagctcc actacttcta
     9421 cccccctact cctggcaata tctactgtat ggtcagtaga tccgtcactt accactacaa
     9481 tctggtttaa gaaaggaata gcttttaaaa catcaataat agggccgatg gttttttcct
     9541 cattataagc agctataatg gctgtagttt tagtatttac atccataatt caggcttttg
     9601 gagccaattc ctcctttaat tttaaaagaa gagttaaaac ttcttccctt tttgtctccc
     9661 atctctcatt attatgacga actttgattt ctatctttcc ctcttgagac attttttccc
     9721 ccacaatcac ttgaagagga aaaccaatca aatctgcttc gctgaattta taaccagcgc
     9781 tgaattcccg atcgtctatc actacctcaa aatctttttc ttttagctcc tggtaaattc
     9841 cttctgccgc ctttctttgt tctactttct tccagtttac tggtattatt tccacttcat
     9901 aaggcgagat gctaataggc cattgaattc ctttttcatc atgataagct tcaatagctg
     9961 cagccatagt cctgccaata ccaataccat aacaacccat gattagcggc ttttcttccc
    10021 catccttatc tacaaaataa gctttcatag gctcactata tttagtcccc agctgaaata
    10081 tatgacctac ttctattcct ctcttttctt ctaaattagc tccacaattg gagcattttt
    10141 cctcatcttg agtagtacta aaatcacaac tcgggcatac tgaaaatttt tcctctccag
    10201 aatcagccag aatgagaaat tcatgagata cttctccacc gattacccca ctctcagcag
    10261 gaacggcttt ataggataaa ccacaacggc tgaaaacttt acaataagca ttgtacatct
    10321 tttcataact aactctcaac ccctcaaaat ctctatcaaa actatataag tctttcatta
    10381 tgaattctcg agctctcatc accccaaacc gcggtctcat ttcatctcta aatttagttt
    10441 ggatctgata taataaaagt ggtagctgtc gataagaatg gatttcacga cgggctaaat
    10501 cggtgattac ttcttcgtga gtaggaccta aacaaaaatc tctgtcttta cggtctttta
    10561 atcggatcaa ttccggccca taaatttccc atcttcctgt ttctttccac aattctccag
    10621 ggtgcaaggc aggtagaagc aattcctgtc cccccgctct atccatttct tctttaacta
    10681 ttctagtaat tttattaagg gtacgcagtc ctaaaggcaa aaaaatgtaa acaccagaag
    10741 aaagctgtct aatcattcct gctcgcaaca tcaaactgtg gctcgctatt tctgcttcct
    10801 ggggattttc ttttaaggtg ggaacaaaca tcaaagacat cctcaaggtc ctttctcctc
    10861 catttctttt ataatcaaac ttatcaattc ttcttctgct tcttcctgcc taactatttt
    10921 tattctttcc ccccgggaaa aaagaagtgc tttttcttga ggggcaaaag caagacccca
    10981 atctgcttcc ttcgcttctc ccggtccatt cacttcacaa cccattatag ctactttacc
    11041 cactattttt atttttctct ctcttacttt ctgtttgaaa tctctagtta aggctactac
    11101 atctcctttt cttcgagcac aagtggggca ggaaattatc tccagccctt tagaccttag
    11161 cttcaagacc tctaaaatac tccaagctac tcgtgtctcc aaaacaggat cagtagcagt
    11221 taaggaaacc cggatgttat ttcctattcc ctctcgcaat ataattccca gagcaataga
    11281 agatttgaca attccttctc ctccctcccc tgcttcagtt aatcccacgt gtaaaggata
    11341 gggaaaggcc cgacttaatt tctcgtatat gatgatagtt tcttccacat cagtggattt
    11401 agcagaaagg attatatttt taaaattatt tttctctgga atagatacca actcctccaa
    11461 gctaccaaaa agtgcttcta cccgattttt tccccgataa tggagaggaa gagacccagt
    11521 attagctcct atacgaagag ctaaattttt atcctgtaag atttttatca attctcccag
    11581 ttttttctta ttacctgccg tccccggatt tactcttact gcatctattc ctaactgagc
    11641 tgcttttatt cctaagtcta aattgaaatg aacatcagct attaaaggga tgtctattcg
    11701 ccttttaatc tctaaaagac caagaagaga ttcttcatca ggcacagcca gacgaaaaat
    11761 ttcacaatct tcttctatca ccttctctat ttcttctatg ctttctttaa ctttagcagg
    11821 actttctctc cccattgctt ctacccagat ggggtttttg cctcccactt ttatttttcc
    11881 cagagaaact tctctggtat tctctctttt catttcccta tcaccattct catgatatct
    11941 tgataagtgg caaagatagc taaagaaaag attaaaatta ttccaataaa gtaaacccag
    12001 cctatttttt ctggttcaag gggtttacct cgaatcttct cgtagaggaa aagtaggaga
    12061 taccctccat ctaaaacggg tataggcaga atattaaaaa gagccagaga tacacttact
    12121 actgcagaga acatgaggag attcaaaaaa cctaattgag ctgcttgacc ggctatttga
    12181 gctattccca gaggaccagc cacttccgcg gggatttttc ccagaataat ataaagcaaa
    12241 cctagagcag taagaacaaa ccagctaatt gtcgtcttag tactgtgaaa taaagttaaa
    12301 agaggattta atttttggtt ttccaccccg aaaatcactt gaataagtgc ccgtttttct
    12361 gtttcgttcc actctggaat tactttaagt cttatctctt cttctctcct tttaatagtt
    12421 aattctattt cctccccagg atgggaagaa ataacactta ctacttcatc cagatcggaa
    12481 gcagactttc cattaacctc taaaattata tctcctgcct ttagcccagc ctggtctgcc
    12541 gggcctccgg ccaccacttc ctgtactttc aggttaccag tgggaattcc cgccacagtg
    12601 aaaacaataa ataataaaac tatagaaaga actatattca taataggacc agctgctaca
    12661 ataagacttc gttgccatag aggtttatgg tcaaatcttt tctccggagg agtattagct
    12721 tcttcatatc cttcaatgac atttccctcc attcctccca ttttcacaaa cccccctaga
    12781 ggaattagac ggatagaaaa ttcagtttct cctcgggtaa aagataccag tttggggcca
    12841 tagcctatgg aaaaacttaa aacttgtacc ccaaaccact tagccataat gaaatgcccg
    12901 aattcgtgga ctattatcaa aagactgatg acaaagataa aagctaatat agttattatc
    12961 attgaatact ctttctttct aaaattaatg tttgagctga ttgataacct cggcgataga
    13021 tagcttttac ttcttctaaa ctccggggag ggttaatttt ttgttctaaa attctctcta
    13081 aaagaagaga tatttctcca aagcagattt ctcgggagag aaaagctctt accagagctt
    13141 catcagctcc gcagaaaagt gctggatacc ccttccctct tttgcctgcc tcccgagcta
    13201 aataaaaacc tgaaaacttc tctggtaccg gatagctaag attcaaagat ttaattcggg
    13261 agaaatccag gctttgaaag gggtttgcct ctctcttttt caaagaaata gcgtttagga
    13321 ttactgacct catatctggg ggggaaagca aggctaagga gaacccatct ctcaacccta
    13381 tcagagcatg aataaagctt tctctttgca ctactacttc tacctcctcc acgctgaatc
    13441 cgaaaagtga agaagcttca attacctcta atcctttatt taccagatta gctgagtcaa
    13501 ctgtgatttt agctcccatt ttccagttag ggtgggccaa agccatttct ggagtaatat
    13561 catcccatct cccctcccat tcgtaaaatg cacctcccga agcagttaaa taaacccagg
    13621 aaatctcttc tcttttaacc cactttagca tttgccatag agcattatgc tcactatcta
    13681 gagggatcaa gttttctcgc tctttaagaa ggtgagactt tatttctcca gctagtatga
    13741 taatttcttt gctggcaacc cacaagatgt gagatgtatc cataagtttc caaaacaaat
    13801 ccaaaaattc tatcccgctt gcagcaaaga aaaccccttc cacttcttga aaaatgactt
    13861 cttctaagag gtcataatca gttataagat tggtagatga gtttttccaa aaatcttttc
    13921 taccctgagg attaaaaaca taaggaacac taaattcttg agcctgctct cttaaccttc
    13981 tttccttgga atacccggat ataagtataa tttcaaaatc ttttcttcga gctaaaacct
    14041 ctaatatttg ttctcccaag taaccagtag aacccaagat ggctactttc attctcatcc
    14101 cccccaggat aatcttaaaa gataaactaa aggcccgacc aggaaaaaag aatcaaaacg
    14161 gtctaaaatt cctccatgtc caggcaaaaa attacctgaa tttttcactc ctgccctcct
    14221 tttcagtact gactcccaga tatctcctaa aaaagaaacc aatgctatcg aaaatccggc
    14281 taaaagtccc tgaaagagag agaaagaaac aggaggtgta acaagaatta aacccgtaag
    14341 agttgccatt cctattcccc ctgccagacc ctcccagctt tttcccggac tgatccgagg
    14401 gactatttta tgtttacccc attttctacc taccaaataa gcaaagatat cgtttgtcca
    14461 cacgggaagt gcaaaaagaa ttatactccc tcggtcataa gcaataccca tttttaccca
    14521 aaaaaaaggt aacaaagaac agtacagaaa tcccagaaga taaaaagcta cttgctctac
    14581 tatttgttca ggctttttaa gtccccatcc tattaatact agaaatagaa tatatcctga
    14641 aaaaagaatt attttataat cgatgttgat tagtgaaagg tagagaagaa caagggaagc
    14701 cagagaaaac cacagccatg gagtccattc tcccctttca taaaccagct gaccatattc
    14761 tctcaaagaa ccgagagaaa gaaagaaaaa aactaaaatt aaagtctggg gagaaaaaag
    14821 gattaaaaac aggaaaacag gtaaagccca gattacactc caggcccttt ttttcaaatc
    14881 tgatgactga gtggtggcct tcaaactcta cccccaaaat tccttttccg ccccgtaaaa
    14941 tcctgaaaag cctggtgata ctcttccaca gtgaaatcag gccataaagt atcagtaaac
    15001 catagctcag tataggcaat ttgccagaga agaaaattac taatcctttt ttctcctcca
    15061 gtacgaatta aaaggtcggg gtcaggttga tttccagtat caagataaat actgaaattc
    15121 tcttccgata aagaggtaag aagagaatga tctgtctgat aaatattctt taccgccctc
    15181 aaaatttcat ctcttccccc atagtttatg gctagattaa gattcaagtt ctcacccttc
    15241 agtgtggatt tttctaaatc atttatctgc aaagtaaggt cttggggaag cccatctttt
    15301 cttcccaaaa attttacttt gactctgttt tcttccagct ccttaccata tttatctatg
    15361 ctctccataa ataatctcat caaaccctga atttcgttta tcggcctctt ccagttttca
    15421 gtagagaaag cataaagagt aagaaaagga atacctgctt ttaccgtttc ttctaccaga
    15481 tttcttatta cttctactcc ctttctatgt ccttccagac gaggcaaacc ctgtctttct
    15541 gcccatcttc cattcccatc catgatgatg gctacgtgcc ttaatattat acctatacgg
    15601 tcattatctc tttctctttc ttttcccata ggctatctaa ctcatttatg tgctcatcgg
    15661 tgagtttttg cacctcagct tgcgctcttt cactatcgtc ttctgatatc tctccacctt
    15721 tttccatttt tttaatttct tcattggctt cgcgacgaag attccttata gctactttag
    15781 cttcttctat ccattttttt gccatcttag ctatttctct ccttctctct tcagtaagag
    15841 gaggtatgat gatgcgaatc gctcctcctt ccacctgagg gttaaatcct aaatccgatt
    15901 tccagataac tttctcaata agagaaagag catttttgtc ccagggttgt attataatag
    15961 tccgggcatc aggagtagta atggaagcta tttgtttcaa tttcataatt gttccataat
    16021 agtcaatttc taaatcttct accagagccg gagaagctct ccctgttctg acatgagata
    16081 attcttctcg agctacattt atagcctgct tcatcctttt ttccacatct ttaactaccg
    16141 attttaagtt ctcattcatt tttctccctc cctactaaag ttcctatctt gtggccagta
    16201 actattttga ccaggttatc tgctttatta acattaaata caataatagg gatattattt
    16261 tccatacaaa gagaaatagc tgtggaatcc attattttaa ggccccgatt caaaacctct
    16321 aaatagtcta agtggtcaaa tttgtaagca tttttttcca tgagggggtc agcttcgtaa
    16381 attccatcta ctttagtagc tttgagcaaa gcttctgctc gcacctcggc tgctcgcagg
    16441 gcagctgcag tatcagtagt aaagtaagga ttaccagtac cgccggcaaa gataactatc
    16501 cttccctttt ctaaatgtcg gatggctttt ctcctgatat agggctcagc tacagctcgc
    16561 atttcgatag cagtttgcac ccgggtggga atcccttcat tttctaaaaa atcttgcaag
    16621 gccagagcat taataactgt agctaacatc cccatatagt cggcagttac tcgactgata
    16681 cctttatccc ctgctgttct acctcgaaaa atatttcctc ctcccactac tatacaaatt
    16741 tctgctcccg cattttttac tttttcaact tccttagtta tataatccag aacctgaata
    16801 tcaataccaa aagggagaga ccccattaat gcctctcccg ataatttaat caaaaccctt
    16861 ttaaatctta aaggagatga ttgttggtca ctcattgtct tcgcccagtt tgtagcgcac
    16921 aaacctcctg accactatgt tttctcttaa tttggcaata gtctccataa gaaggtcttt
    16981 cacttttttt tctggttctc gaatataagg ttgttccaga agacaatttt cctcgaagaa
    17041 ctttttcatc tttccttcca caattttttg tttgacttct tctggtttat tactgtcttt
    17101 caattgttcc tggtaaattt cttcttcttt cttttttatt tcttctggaa cattctctgg
    17161 agctacccag cgaggagctt gagcagctac ctgtaaagtc agctccttta ctaagtaacg
    17221 aaactcatca gtgcgagcga caaaatcggt ctcacaattc acctctacca ggactccaat
    17281 tttaccatca gtatgaacat aagctccaat caacccttct tgcgctactc tgccctgctt
    17341 tttagctgca gcttctaaac ccttgcgacg taatatatca caggcttttt ctatatctcc
    17401 tcctgcttct tctaacgctt ttttacagtc catcacccca gctccggtta aatttcgaag
    17461 ctcaaaaacc tctttagctg tagcactcat cagttatcat catcctcctg ttcttctgtg
    17521 tcttcctctt caatttcttc taatcctaag tcctggagct cctctaatat ctgtgaagca
    17581 tcttcttctt gagctacaag ttctgtttct tttttctctt cttccacctc ttccggaata
    17641 gctattcctt ctgtagctat catttcttgc ccttcgccag ctagtctctt tccctccagt
    17701 atggcatcag caacaagagc agaaaagaga cgaatagccc gaatagcatc atcatttcca
    17761 ggaacgggat aatcaatttc atccgggtcg caattagtat ctacaatagc tataatagga
    17821 atatccatct tccttgcttc taaaactgca tttctttccc ttcgggggtc aaccacaaaa
    17881 agagcatcag gtaacctttc catgtctcga ataccactca gatatttttc caggcgagtt
    17941 tttcttttct ttatctgcat aacctctttt ttgggaagcc gttccagtat ccctttttct
    18001 tccatttctt caatcttttt caaccgttca atgctttggc gaatagtaga aaagttagta
    18061 agcattcctc ctaaccatct ctggttaaca taaaacatac cacaacgttt agcttcttgc
    18121 tcaatagctt cttgagcttg ttttttagtg cctacgaaga ggaatgttcc tccctcccga
    18181 gccaaatcgc gaacaaaatt ataagctttt tccgctaatt ctacagtttt ttgaagatcg
    18241 atgatataga tgttatttct ttcagcaaaa atatagggtt tcattttggg gttccaacgt
    18301 ctggtttgat gtccaaaatg aaccccggct tccaaaagct gtttcatagt gactaccatt
    18361 attctaacct ccttctggtt taacctctgt agagttgctc tttagagcac cagaaatact
    18421 ctacatgtgt tttaaccaaa ttaccgcccg taagtatacc taaagaacag aattttttca
    18481 actgaaaata aaaaaggatt aaagaatata tcgagtaata tcttcatctt gcaaaacacc
    18541 agcaagctgt ttctcaacat aatctcgatt tataattact ttttgccctt tcaagtaggg
    18601 agcacggaaa gagacatctt gtaaaagtct ttctactaca gtatgtaatc tccgagcacc
    18661 aatattttcc attctctgat ttaactcttg agaaatatga gctatttctc gtagagcatc
    18721 tttttcaaat tctatgctta ccccctcagt ttctaataat gctttatatt gcttggttaa
    18781 agagttaaag ggctcaatta atatccggta aagatcttct tctgttaaag aagataactc
    18841 cactcgaata ggaaaccttc cttgcagctc aggtaaaagg tcagagggtt tggtctgaga
    18901 aaatgctcct gcagcaatga aaagaatatg gttagtcctc actgggccat atttagtaag
    18961 aacagtagaa ccctctacga taggaagcag gtctctttgc accccacctc tggacacatc
    19021 tgggccatag ccacctcctg gagaaacaac tttatctatt tcatcgataa acactatacc
    19081 ggtttcttct actcttctta ttgcttcact gcttatctct tccatatcaa ttaacttctg
    19141 tacttcttgg ttaaaaagta tctctcgagc ttctttaact tttgttttcc tcttcttctt
    19201 tcgaggagga aagaccccac taaacagatc ttgaaagtct attcccaaac tttccattcc
    19261 tccgccagct ataattccca gaggaataga actttcttct gtctcaatct cgatgaaacg
    19321 ttcttctaat tctccccttt tcagtttttc cctcattttc tcccgggtag aagaaagagg
    19381 tctaggccac actacatcta cccgaggaga ttctgaagct acagtatctt catattccaa
    19441 agaagaggga ttaagagcca gaagataatc taagatttgt tcttctacta tttctctggc
    19501 tttttcttcc tgacttttta tgcgttcatt tttctccatc cttactgcca attcggttaa
    19561 gtctctaact atggattcta catcttttcc tacgtatccc acttcagtaa atttagtggc
    19621 ctctactttt aaaaaaggag cattagttaa tttggcaata cgacgggcaa tctcggtctt
    19681 acctacaccg gtggggccaa tcataattat atttttagga gttatctctt cagcaatatc
    19741 aggaggaagc attcttctcc taatacgatt tctcagggca atagctacac actttttagc
    19801 ttcatcttgc cctattatat atttatttaa ctcctcaact atttcttccg gagttaagct
    19861 ttcttctatt aaaggcatct ttacccacct cgactaaaca gtttctatag tcacattact
    19921 attagtgtat atacagattt ctgaagcaat ttgtaaagaa aggcgagcga tctcttcagc
    19981 ttctttgtca gtttctctcc acagcgcttt agcagccgcc agagcaaagt ttccgcctga
    20041 gccgatagca gctatcggct catcaggctc caatacgtcg ccccttccag aaattatcaa
    20101 aatatgctct ttatccatag ctaaaagaag agcatctaat tgcctcaaaa ctttatcagt
    20161 tctccagctt ttagccagct ctatggcagc acgcgggaga ttaccactat agtaattaag
    20221 tttttcttcc aaacgttcta aaagagttaa tccatctgct ccactaccag caaaaccagc
    20281 taagacttta tcttgataaa tgcgccgaat ttttcgcgct cctcctttca gtatggtttc
    20341 attcatagtt acttgcccat cacaagccat agctccctta ccatttctta aaacagcaat
    20401 aaccgtagtt gacttaatca tcgttcttca ccttcttagc atgaggatga gtttcttggt
    20461 aaactctctt cattttatcc cagtttaagt gggtataaat ttgagtagtg gaaagactgg
    20521 aatgtcccag ggcttcttgt accatcctta gctctgctcc tcctcctaaa aggtgagtag
    20581 caaagctatg acggaaaaca tgaggggaaa tccgttttcc acccaatccc gcctcttttg
    20641 tgattgcttc taaaattttt cttgctcctc gggaagttaa aggagtgcct ctctggctca
    20701 aaaatacttc ttcccgggaa aagggacaat gacttagata ttctctcaag gcttcctgag
    20761 tactttcatt aaaaggaact attctttcct tccctccctt tccctttacc tttaccattt
    20821 tactcgccaa gtcaaggttt ttgattttca attgagttat ttcgccgact ctcaagccac
    20881 tggagtagag aagttcaaaa aatgcccagt tcctgagcga aggaaaatcc tgcttttctc
    20941 gtgccttttg ctttaagtga ttaagcaaaa actccacttc tcctacagtc aaaaaattag
    21001 gtagtttctt ctctttattg agctttccca acccatcggt agggtcacaa tcgactatgc
    21061 cccgacggcg gagaaagcgg taaaaagtgc gaagagcaga aatacggttg gcttgagaaa
    21121 tccttttaag gtgatgatgt gttttgagat aggctaaaaa atcggccagg tcttgagttt
    21181 tagcctgaac caacgtaagg tctctctctt ccagaaattg agagaattga cgcagggttc
    21241 ttccgtaatt atctactgta taaggagaat atcttctttc tttttgaagg tattcgagaa
    21301 aaatatagat atttttttca ttcgccggag agctcaatag atataggcac cccctcccag
    21361 tctccttctt ttcggattag cttttctatg cggttagcca ggagctcttc ttcccttttt
    21421 gtagcagaaa cattaaccga taaataaaaa cgaggaggag caatatcttt ttgatgcata
    21481 taataagtac ggaaggttct tcctcctact ttccagctct cttcgtttat tttctctttt
    21541 attttttcat ttagaaaact ggtttttatt ctctgataat agcgagtagc aattccagca
    21601 agaacagaga aaatatctga agagacagaa ggagaaagag cacatccaca taagaaagaa
    21661 gcataattca agaaacccaa atcttgacgg atttcttcta tcagtctctt cgcctgtccc
    21721 cgggaatgat ggtttaaaac taagtcagtt ttattaataa acaccagaca tccttttttt
    21781 tcttctttta ttttttgagc aatcctttta tcctgacgag taatcccctg aatgggatca
    21841 attaccaaaa gagcaataat ggcatcctgc aaactttcct cagatctcaa agcagcataa
    21901 aaatcaattc tctctttaat tttaggtcga gttatcaatc ctggagtatc cattaattcg
    21961 tagtaaccaa agctggtgtc taaagtggat agaagtgcat ctcgagtggt acctggaaca
    22021 tcacttacaa tggaccgctc ttttcctaag agagaattat agagggtaga cttacccaca
    22081 ttaggtttcc ctacaatagc caccgaaatg cgatgcctgt caaaataaat ttcctggttt
    22141 ccttctacta tcctggtgat tgttttacgg agttctttaa gaccatctcc gtgagcagca
    22201 gaaatctgca taacagattc aaaacccagt gaaaaaaact cagcaggcag ggcttcgctg
    22261 gtcattcctt ctcttttatt tattaccatt attaccggat tcttaatctt ccgcaaatgg
    22321 gaagccagct cccaatctat actggtaagg ttctcccttc catccaggag ccagaggaga
    22381 agatcagctt ttcctattac ttcccaggtt ttctcttcta ctttcttttg catttcattc
    22441 tgagaatcta ttagaatttc taatcctcca gtatccacca actttatctt ctttccctct
    22501 atatctacta tattttccaa gaagtcccgg gtaacaccag gctgcggatc aactatagct
    22561 attctcctcc gggaaagatg attaaatagg gtggatttcc ccacatttgt ttttcctatg
    22621 atagctacca agggtatttt ctctttcata aaaggaagct tcccttactt ttaatttttt
    22681 tactacatcc tccactgaaa aatgaggggt agaagttcca cttaccacta atattctatc
    22741 ttccttacct ccatattcat tttccagttc ctcagctctt tctacccaga tagattctat
    22801 tccccacctc cgggcagtaa aaaataaagc tctggtgtta gaactatttt tccctcccac
    22861 caccactact ttgttaattc tgtgaagctt gatgctttct atcaaatctc tctgccgcat
    22921 ctcggtttca gcgcacagag tatcaaaaac ctcaagatta aggtgaggaa ataagccctt
    22981 tatctcctga gtaaactcct cttttatttc ccggggaaaa gtggtttgtt ctaccactgc
    23041 tattttttct cgaggagaaa atttattcaa ttccccccat tcttctctat tttccaaaat
    23101 cagggctttc tgagaaatga aactcagtag agccattacc tcaggatgct cagggctacc
    23161 taaaataatt agactgtacc ctttttcctc taattcttga gctaaaatct gtactttctt
    23221 tacccggggg caggtggtat caataatttt tatctggcgg ttctccaaaa attctttagt
    23281 ttctttttcc aatccatggg aacgagtaac cacccaggaa aaaggaggta tgtcttcagg
    23341 atcagtaact attattcctc cctcctcttc cagcatcttg accgcctgag aattatgaac
    23401 taattctccc aaaaaataca tcggcttccc ctgggataga gcacgaagag ctaaatcaaa
    23461 agctctcctt accccagggc aaaaacctat cctgtttgct acttgtactt ccatcttagt
    23521 tttccaccag tttataaagt ttttctaaca cctcatctat acttaaaaaa gaggtatcta
    23581 tttctatagc gtcttctgct ttttgtaaag gagcatcatc ccgggtagaa tctataaaat
    23641 cccgcatcag cacgtttttt aaaaccattt tataatctat gttattcccc tcattttttc
    23701 tttctttcca ccggcgataa gctcgaatct ctggacgagc agtaagaaaa actttcaaat
    23761 ctgcatcggg caatatcact gttccaatat cccgcccttc ggtaaccgaa gggcgggata
    23821 gagcaatttc tcgctgcaca gagcgcagat aatctcttac ctcttttatt ttagctaccg
    23881 gagaaactaa ggcatctacc tcgggttggc gaatatcttc ggttacttct ctgtcatcca
    23941 aaaatacccg aaacttgtct tcccaggaaa cttttatttt tatttgagta agcagtgatg
    24001 aaaggttttc ggaatcctcg aatgggatat gattttccca gaaataaaga gttaccgctc
    24061 gatataaagc tccagtgtca agataaagat atttcaagca ccaggccaat ttttgagcta
    24121 ctgtgctctt tcctgctccg gctggtccat ctatagcgat atgttctttc atctttccct
    24181 tatctcccat tctccatacc caatttttcc taaatcttcg aagaactcag gatagcttat
    24241 tcttacacaa tctatgtctt ccactatcaa atcttcctcc cccactattc ccgctattac
    24301 caggctcata gctatccgat gatctccgta actatctacc cttcctcctt gaaaaacgca
    24361 aggtccctga atcagaagcc cgtctttcat ttcttctact tctaccccta attttcttaa
    24421 tccttgagct atagctttca gacggtcagt ttcttttact cttagctctt ctgctccaga
    24481 aataatcgtg gttccctttg ctttagcagc cagaaccgca atcacaggta tttcatcaat
    24541 cacaagaggg accaaatctt tttcaatttt tatccctcgc aaggaagaag attctacttc
    24601 tatgtctcct cgacgttcac caaactcttc tttttcccca attattttaa agtttcctcc
    24661 catttcccga agacaggcca aaaacccgga tcgagtagga ttcattccca catctttaat
    24721 tagaatatga gattgaggta aaagagtagc cagagcaatc aaaaaagagg ccgaagataa
    24781 atcgccagga agcgataaat aagaagcaga aatagaagaa ggaggttcaa cctcaattac
    24841 attacccttt ctttctattt taactcctga ataacggagc aggttttcag tgtgatctcg
    24901 agaaagcaaa ggttcttcaa taaaagagac tccttctcct cgaagagcag cgaaaatcag
    24961 agccgacttg acttgggcac ttggaacagg aagactataa gaaaaagctt ttaccctatt
    25021 tttacctcgc acaaaaaaag gagggaaaga atctttattc cttcctccca catctacgcc
    25081 agtgaggcgt agaggctcca taatcctctt catcggtcgt cggcgaagac tattgtctcc
    25141 agtgaatatg gtaagcccct ctatcccggt agctataccc gctaaaagcc gcatagtggt
    25201 tcctgaattt ccagcatcaa taacatcttc tggctctttt agctttccta atccttcgct
    25261 tttaaccgta attctctctt tttcttcctc tttaattatt tccacccccc agagcccaag
    25321 actggcaaga gtagaaagac aatcggcaca aaaagaaaaa ccatcaataa ccgttacccc
    25381 ctggctcaga gctcctacca tagctgctct atgacttaaa gatttatccc gaggagcacg
    25441 aatttctcct tctactttct ggggaaaagg atgaactaaa acttccctca taggtttttc
    25501 cccctgttca agtcttccct taaagaagat gctttcccta ggtaaaggtg atgaatatca
    25561 gtctggttct tatcagtata ggctaaaacc agaagacgga tacatttttc cagagctcct
    25621 tctactttca tttcctgggt gcagagaaga ggaacatagc gaaactcctc tctttctcta
    25681 ataacttcgg caggaaaagt ggaatgtaag tcaggagtgg cagtaataaa aacccctact
    25741 atctctgaag gaaagatatc attttctctc aagagaaggt caaacaactc cttagtagct
    25801 tttttaatct cctctttatc atcctgttct actgtggttg ctcctcttat tcctctgact
    25861 tttttttcca ttttaatact caaaacctct tctatctgat attcctttat cccagggatg
    25921 tttaaccagc tttatttctg aaaccaggtc agcaatagca attaactctt ccggcacttc
    25981 tcttccagtt ataattactt ctaccccggg gtctttttgt tctagagctt ttctgaattc
    26041 ctcccaggtt aaaagatgat aataaaaagc ataaggcaat tcatccagaa ttaccacatc
    26101 gtattctcct ttttccagag cttcccgcac catctgccag cctaggaaaa attctctccg
    26161 gtcttcaggg aaaacttctc cagtaatgaa tttcccacta ccaaattggt agacatttac
    26221 tcctggtaaa agttgcaaag atgctaattc accggtaaac ttcctcttaa agaattgtac
    26281 aagaaggact ttcattccct ggcctactgc tcgcaacaaa gctcccaaag cagcagtagt
    26341 tttacctttc ccttctccaa aatatacctg aagtaatcct gatttatctg ccaaattttt
    26401 cctcccgata tttttgtaag agctgaattt cttcatcgga gagataacgc cattccccgg
    26461 gaagaagatc tttatctaac aaaagaggcc ccataccaac ccttattaat cttaacacgg
    26521 gatgtctcaa ataatcaaac attatccgga tttcatgctt ttttccttcc gagatggtaa
    26581 cctctaccat actccagttg gagtgagttt ctttaatttc accttgctga atgtgataga
    26641 aaattccttt aacttcaatt ccttttttta agaaggaaag ttttttctca cctatatttc
    26701 cactaacgta taccaggtat cttttttcca cttcaaatcg ggggtgagta agaaaatagc
    26761 ttaaggcacc atcattggtg aaaagcagta gtccttcgct ttcgctatct aaccttccta
    26821 cgggaaaaat tcgagcagtt atcccggaaa ggagctccac aaccgttcgc cgtcctcgag
    26881 ggtcacagag agtagtgaga taatctcgcg gtttatacag ttctatataa accagctctt
    26941 ccttttttcc tactatttta ttctctactt ttattatttc acttccactt actctctctc
    27001 ctggattggt gaccactcta tcattgatgg taaccttgcc ttcttttatc aaaaagtcac
    27061 actttctacg ggaggctatt cctgcttgag ctaaaaatct atttaatctc tccatttttc
    27121 ttctcttgaa acaaattctt cagtctttcc aattcttctt cgctttctat tccaaatatt
    27181 tctaaaaact tatcagtagt tttatacaga aaaggtttac ccggaaggtc accctgacct
    27241 gctacagtta ttagtcctct ctccaataga gtgcgaatag tttgtgccga atccactcct
    27301 ctttttagat cgatctgagt cttggtaaga ggttgataaa gagaaataac ggccagagtt
    27361 tccagagctt ttcgagaaat agttttagta ctatttactt ttatcttctc ttttaattcc
    27421 tggccatatt ctggtttaac tatcatctgc cacctaccat ttatctcccg aataacgaga
    27481 gctccctctc ttttttcata ttcattacgg agctcttgta aagttttaat tacggtttct
    27541 ttatctgttt cggttagttt tgatatttct gaacaggata ctggatgaga agaagaaaaa
    27601 agtaaagctt caatcagatt tttcaactgg aggacaggtt tttccgaaat atattcggcc
    27661 attttctccc cttttcattt gcagtttttt tctaaaaact aagtccagta aaattaaaaa
    27721 agtagcaata gtagttttcc gatctaggca atcgtggaaa aattcatcca tctcccagag
    27781 ttctttttct tctatcattt ttttaattgt tatttttctc tcttccagta atttctgaaa
    27841 ctgggaatcc agtgatacat caggaggagt ttcgggagtc aaataaagct cgtaaagaga
    27901 taaaagctct gccagagtta gtttttctaa ctcttttact tctctatttt gaaaagcttc
    27961 ccatcgcttt tcgggagctt tcaaagtact ttgctctcgc tcctctaagt taaagagaag
    28021 ctgggtaaaa agaagaaatg cttcttcttt ctcttgtgac aggttagctt cttcgttttc
    28081 ttcctctccc tttcctaatg cttcatctaa ttgactttcc aaaaaggcga gaacttcttc
    28141 ccacacttcg atacattgag gaaaaggagt ttcctggaga agttttttcc actgagattc
    28201 aggatttaaa atcatagttc aatttcatag cctctttggc ttcttctaag gtagctcgag
    28261 ctaattgacg agctttttta cttccttccc agagaatctc tttaactaat tgtttttctc
    28321 tctggtattt ttcccggtga gggaccactt tttctaaaaa ccttgtcatc agctcagcta
    28381 attcttcttt acaacttacg cacccccatt ttccctctcg acaatctttt tctatctctg
    28441 taagccgagg gttagcaaga agacggtgga gggaaaacac attacatcgc tcaggatgcc
    28501 cagagtcaga tagacgaatt ttttctggat cggtgaacat ggaacgaact ttctttttca
    28561 cctcttctgg agattcggag aggccaatat aattatttaa acttttgctc atctttttcc
    28621 catctatacc cagaatgcga ggagcttcag taagtaaagc ctggggttcg gggaaaacct
    28681 ccccatataa atagttgaaa cgacgggcta tttcccgggt aatttctata tgaggaacct
    28741 ggtcttcgcc tactggtact ttaactgctt tataaatgag aatatcggag gccataagta
    28801 ctggatagcc taaatgtcca taccccactg cctctttgag acccatatcc tgaagttgct
    28861 ctttaagaac cgggtttctc tctaaccatc ctaccggggt aatcatggaa aataacaagt
    28921 gaagctcagc atgctctgga acccaggatt ggagaatcaa aatactcttt tctggatcaa
    28981 tccctactgc caaccaatca gcggccattt ctatgatgtt gttttccagc tgggcagtgt
    29041 cttcaaaaga agtagttaaa gcgtgccaat cagtaatgcc aaaaatgcaa tggtattttt
    29101 cttgcagttc tacccagttt ttcaatgctc caaaatagtg accaatgtgc atcttaccag
    29161 taggtctcat cccgctgaaa atagtttcct tatctttcat ttgtttcatt cctccagatt
    29221 ttttctacta atatttcttc ttcctccttg cttttaacta agccgtcaat ccttgccagc
    29281 ttcaactctt ccagaatttt agcataaact ggtccggcag gaattccttt tctcctcagg
    29341 tcttctcccg taagacaggg aacgattttt ctccattctt ttaaatattg ctctactctc
    29401 tttctgggaa gggagtcttc cccccattta gcctcaagga gaagtaaaaa ttcatccgac
    29461 aggggacgca gaaggagata aacttgagaa ttgggtaacg tcccatcaag aagttttatc
    29521 tctaattctt ctttattatc taagtacact ttaatcttac ggacacaaac tttagagaga
    29581 gctaatttat ttttcaattc ctctaaataa gcggggtcca tttcccccca taaaggagaa
    29641 agtttagcta aaaaataatc taaagatgga gaagaaaact gatctaagag cttcaccaac
    29701 ctctgataga taggttcaaa ctctcttctc caagtactgc ccgggaaaat gtagggaaac
    29761 atatccagtt ggtataaacg ctctaaggag cgataaaaat ccgataaacg caggataaga
    29821 ttaatttctt cttttaatcg atcgggttta attttattca acaattcttc tcttgctgtt
    29881 tgcttgagca aactcatggt aaagggttca atttcaaagc catacttttg ctcaaaccga
    29941 acagcacgga tggcacgggc aggatcctcc acaaaactta gaggatgaag cactcgcaaa
    30001 attttatttt ctaaatctct taatcctcca aagaaatcga ataaatctcc ccaatcatcc
    30061 gtcccgatac tgatagccat agcattaata gtaaaatcac ggcgaaataa atctttccgt
    30121 aagttactat attctacatg aggaggagct ccaggatagt cataatattc cgaacgagcc
    30181 gaagcaaaat ctactcttct tccatctttc agaaacaaaa tagccgtccc aaaaggagga
    30241 taaggaacta ctttacctcc taatttctgg actatagttt gagctaactc tatagcgtct
    30301 ccttccacta ctatgtccaa gtcataacca ggaaacccca gaattatatc tctaaccact
    30361 cctcctacta aatatgcttt tacccccgct cttttagatt catcaccaat aaagtgtaaa
    30421 agttgtaaat cgagaggaga gaaatggaga tatagttttt cagccaggtt aagacgagta
    30481 agttcttgta ctactgaatt ttcccgctgg tgtaaaaccc gtagaaggtc actgcgggtt
    30541 ataattccta aaagagtatc cccttttacc accggtattc ttccaatatc tttttctacc
    30601 attaagttac gaactttaaa tatagaatct tccgagtcta cagtaacaag ctgggaagac
    30661 ataaaactct ttactggagc attttgcaat ccatgacgaa aagctttctc cacatctcga
    30721 cgagcaatca tgcccactaa ttttccatct tcatctacta ccggtaatcc agaaaaacca
    30781 aaccgcataa gaagaagaag agcctcgccg atagtagtag agggagaaac cacctttacc
    30841 ggataggaca tgacttcttt tgcccgggta aaagaaagag aagctgctaa agcttctcga
    30901 atcatttcct ctacttcttc tatttttttt cctttaacag tgagagaagc agcactacga
    30961 tgtccccctc cccccatctt tcccagaatc tttgctaaat ccacttcttc taaacggcta
    31021 cgagcaatga cgtaaacttt attttctgtc tccatcagga caaaaagcag ttcgatttct
    31081 tctaaatcca ttaatttatg gactaataaa gaaatacctt ccacatactc agttaacctc
    31141 atccggcaaa aatgaacggg aatcccttct atttcctctg catgaagaaa attaatcatc
    31201 tttttaagag tttctctttg ttccggagta agctgaatgc cagtaaagcg gtttacaaaa
    31261 ctcactttag caccttgccg aagaagaaaa gaagcgctct ctaaatcagg agcctgagtg
    31321 gtgggaaaag taaaagaacc ggtatcttca taaatgccta aagccagaag agtagcttca
    31381 agtggagaaa gggctatttt ctgctccatc attttttcta ctaatatagt ggtacaggca
    31441 ccatattctt taattatggc ggcttcctct cttttttctc cttcagaatg gtggtcaaaa
    31501 atgaccactt ctactttacc atctctgatt agctctcccg ccttattcaa gcgttcccag
    31561 taagcggtat caactacgta tattttgttt actttttccc agtctacttc tttttctttc
    31621 aaaatcggaa aaaaattacc ataaagagag agaaaatgac ggacgttgcg attaactgtt
    31681 ccccagatca ccatctgagt tccaggaaaa agcctggtaa ccgcaaacat agaagctaaa
    31741 gcatcaaaat ccgttccttc gtggctaact acgatctgca tttttactcc attcttccaa
    31801 aaaagaagaa aataatctct ggagaagagg ctgaagagag acggcaatct ctaagacctc
    31861 ctggtggtga ataagagaac cactcatgcc tgctccccag ttggtaacca aagaaattcc
    31921 tcctacttta agacctaaaa aagaggccaa ttttgattct ttaaccgtag acatacctac
    31981 catatcacca gagcgggaca aaaatcgaat ctcagctgga gtttcaaaag aaggacctaa
    32041 aacccctgta tacactccct ggttaagagt aatgcttcct tttcttcctg cttttttaaa
    32101 aatctcttga attttttcat caaaaaaggg aggaggaagc aaaatcgggt caggataaaa
    32161 agtgaagtct acctggtcgg taataataac cacacttcct ggaggaagag aaaaagaaag
    32221 actccctgct gcattggtta agataatagc ttctactcca gctaaatagg aaaaaacaac
    32281 aggaagagtt atctcaaaaa aagaatagcc ctgataaaga tgaagacgac cactaaaaag
    32341 gagaaaggta aaatcgtttt tctttaataa acaaagtttt ccagcatgtc cttctacttc
    32401 agtaaaaaaa ggaatcccca gataaggata ggaaaactca ccgatcagtt ctccctttac
    32461 tgtctcttcc caaccagaac cagctactat agccaactgg ggtttttctg aaaaagagga
    32521 gataatttcc tggaatctaa ggagaatttt ttccggataa tatctggaaa gcaaagcttg
    32581 ttcccggacc attccactcc acctccatca tctgagctat agtagcagca atatcggaaa
    32641 aggtttttct tatgcctaaa gatacacctt gagaattaga ggggttaaaa actaacaggg
    32701 gaacatattc tcgagagtgg tcagtactcg gggtggtagg atcacacccg tggtcagcgg
    32761 ttataaacag aacttcatcc tctcgcagag aagggagaaa agaacttaaa tctttatccc
    32821 aagcttccaa ggcttgagca aacccccgag gattattacg atgaccaaaa acagtgtcaa
    32881 aatcgttgaa attaacccag accaggtctc cttttttgct tttctgtagc tctcgcaaaa
    32941 tttgaaaccc ttccttgtta tcagctattt ttatattttc atttaaccct cttccggcaa
    33001 aaatgtcctg aattttaccc actccaatat tttcgctacc ctggcggaag agcacatcaa
    33061 gtattgtatc tttaggagga ggaagagaga aatcacgccg attagctgtt cttttaaatt
    33121 gaccggggcg tcccacaaaa ggtcgagcaa ttatgcgagc tacagcatgt tcccccctca
    33181 ttatctccct tgccttttgg cacatttcat agagctcagg aaggggaatg acttcctcat
    33241 gagctgcaat ttgcaaaacg ctatcagctg aagtataaac gatgggataa ccagttctta
    33301 aatgctcttc tcctagttct tcaataatca ccgttcctga ggcaggttta ttacctaata
    33361 tttttctccc ccaagctctt tctaattctt ctataataga aggaggaaaa cctcgaggat
    33421 aaactggaaa ggacttttct aatattatac cggctatttc ccagtggccg gtagtagtgt
    33481 ctttaccttt ggatttctcg ttcatcttcc ccgataaagc taagggaatt tctacctttt
    33541 ttacccctgg gatatcatct atccacccca gacccagtct ctctaaagta ggaagagata
    33601 gccctcctac cgcttgggca gtatttctca aagtatgact accttcatca ccataaaaag
    33661 aagcatcggg taactctcct attcccactc catctaagag agcaatgaaa attctcatct
    33721 atctttcttc cctcatccac tcataaatgc caattctagt tttagcatct tttatttctt
    33781 gccggtctaa cattttttga gcttctttct tgcttatttc tcgaagatag attaactcat
    33841 cctcatctcc ggctcgagcc tctgtaactg gtgatacgtc tttggctcgg aaaaaatgga
    33901 ttatctcgct actatagcca ggagctaaag tcaaaggaaa tagatgttgc cagttattac
    33961 tggaaaatcc ggtttcttct tctaactctc gttgagcaca aaatagtggt tcctcacctg
    34021 gctctaaagt tccagcagga atttcccata accactcctt agccggagca cgaaattgct
    34081 taattaaaag aagtttatca tccggggtgg tggcaattat ggttaccgct ccaggatgag
    34141 caataaaaac cctctccatt cctccctggt aagtattctt aattacctta aaaatcccag
    34201 cttctaagag cacttctttt tttatttctt gaggcaaaga aaaatcgttt tcattttctg
    34261 agtaagaaaa aaacaatatc attcctcctc tggagttatc catacgtggc atacattaac
    34321 gtctgaattt ttatttaatt cttcaataat tttttccggt tcccgagtag ctaaccgcat
    34381 aaccacgtag cgataatctg gtttctggtc ctggtaaaaa gtagctaaac tgagaatatt
    34441 aactccgtat tctctgatta ctcctgtaac ttcatggagg accccgggcc tttgaggaag
    34501 ctctaaagtc aggcgtgttc ctccttctct aactcccaga gtttccacta aaatctcaaa
    34561 aatgtccgat tcagtaatta ttcccaccac tcgaccagct tccactaccg gtaaaccacc
    34621 aatctggttc ttattcataa tcagagctgc ttcttcgata ggagtgtcag gttttgtata
    34681 aattacctcc ctggtcataa tttctgccac actcattttg ctcaaaaggt aagtaatttc
    34741 aaaccgactt aaagtggtag ccttggaagg agaagcttcc agaaggtcat tataggtaac
    34801 aatacccacc agctctcctt tttctactac tggcaaacga cgaatatttt tgtccttcat
    34861 aattttctcc gcttccagga cagtagtgga aggaggaaca gtcactacct ccttattcat
    34921 ccggtctttc actaacattt actacccctc ctttttcttc aaagctcttt cctacttcta
    34981 aagctttaaa gttgactgat aagagatttg cttttttccc gactaaaatt ttttccagtg
    35041 ctttctttac tgcagaaaaa ctcaccaccg gttggcgagc tatcaaggct cctaatatca
    35101 ccatattagt ggtttcagca actcctacct cttttcccgc taattcatta gcaggaactt
    35161 gtaaaatttc gatatcccgc cgagagcaat tttctcgaat taaagaagag ttgaccacta
    35221 aaagcccccc tcttctaact aaaggagaaa agcgacgaaa agaaggctca ttcatcacta
    35281 tacaaatatc agggtggtca gtgactgtag cccctatttc ctcttcagaa attattactg
    35341 tacagctggc tgtccctcct ctcatttccg gaccataaga gggaaaccag cttacttcat
    35401 atccttctat cattccagca gtagccagga ctcttcccag aaagaggatt ccctgccctc
    35461 caaaccctcc gattaaaatc tcatgatgca cgagagctat cctcctttac ttttatttct
    35521 cctaaaggaa aataaggaat cattttttcc tctacccacc gcactgcttc ttggggagtc
    35581 ataccccaat tggtaggaca gggagagaga atttccacca gtgcaaatcc ccttttttct
    35641 atttgagtga gaaaggcttt tttgatacat cgcttagcct gactaattaa ccgggggtta
    35701 gtaagagcca cccgggcgat ataggcactt ccgtcagttt gagctaacat ttctcccaac
    35761 ttaaagggaa atccctgtgc ttctgtgttt ctccctaaag gagaagtagt tgttttctgt
    35821 ccgggtaaag tagtgggagc catctgtcct cccgtcatac caaaaactgc attgttaatg
    35881 aaaaaagagg taataagttc tcctcggttg gcacaatgta ctatctctgc tgtaccgatg
    35941 gcagctatgt ctccatctcc ttggtaggaa aaaactactc tatccggaag ggatcttttt
    36001 atcccggtag ccactgctgg agcccggcca tgagctccca taacaaagtc aaggtcaaaa
    36061 aattcgtaaa tatacactga acaacctacc ggtcccactc ctattgtttc ttctgcaata
    36121 ctcagttcat ctataacctc agctataagt ctctgagcca gaccatgatg gcaacctgga
    36181 caataggtaa aaggtttatt aataagagtt ttaggtctct caaaaatcgt tttcattttt
    36241 ggccttctcc ctctaaaata ttggcaatct tttgcaaaat ttcatctacc gtaggtatca
    36301 taccccctac tctaccgtaa aaatttaccg gcgctctacc ttccactccc agcctcacat
    36361 cctctaccat ttgacccaga ttcatctcta caacaagaac tctccccgct ttctctgcca
    36421 tttcctgtac tacccggtaa ggaaagggga aaagagtaat gggacgaaga agagaaagtc
    36481 gccagccttt ttttcttcct tctctcacta cagttttcaa gatacgtgct acagttccga
    36541 accctacaag gagcaagtcg ggattatctt cttctccaaa tacttcccaa tcagtatctt
    36601 cttctgccat ctgctggtat tttttttcta acttgcggtt aaaattttcc agctcttgag
    36661 gatccagttc catacacagg ataaattttc tctccttttc gccctttcct tttactgccc
    36721 aatctttggg aggaagagaa ggtagctcaa ttccttccca ggttaccggc tccatcattt
    36781 gccccagcat accatccccc aggattttca ccgggttacg atatttatcg gctaaataaa
    36841 aggctcgatg agtcaggctt accagctctt gcaccgaaga aggagcaagg actaaagtgc
    36901 gataatctcc atgacctcca cctctggtag cctggaaata atcactctga gcaggctgaa
    36961 tattacccaa acctggtcct ccccggctca tattcaccac cacacagggt agttcagcag
    37021 aagctaaata agagattcct tcttgcttta aactaattcc cggtccagaa gaagaagtca
    37081 tcacccgtgc tccagtagat gaagctccgt aaaccatatt tatagctgct atttcgcttt
    37141 ctgcctggag gaaaactcct cctacctctg gaagacgtcg agataaatat tcacttatct
    37201 cggtttgagg agtgatggga taaccaaaat aaaaccgaca gccagcttga acagctgcct
    37261 ctgctaatgc ttcgttacct ttcattaaaa ttctttccat ttttctactc cctgtaaact
    37321 gaaatagcta catctggaca aacctgagcg caaaaaccac aggctgtaca tttttcttcg
    37381 tcaatcattg tggctggata gtaaccttta gaattaaaat tatcttcgat ggttaatatt
    37441 tttttgggac aaatattaac acacagacca caacctttac agaactcctg attaatgttt
    37501 acctttgcct tagccatttt atctactcct tactccatcc atggtagata caaaaacctt
    37561 tgaatgccaa ccactgcgct atcaaaaata ttccaggata aatttcttac cagactttcc
    37621 cagatgctta caaaaaaaat gggcagactc tctttttgtg aaaaatcccc ggttttttgc
    37681 catcccattt tcaggatatc ttcatcagtg tattctatga gatgagtatt ggagataacg
    37741 gagtgaacgt caagatttaa aacagttcta gctttttcta taaaatcaga gtaattttcc
    37801 tcttctgaaa aaggacgaca gaaattagtc accagtgtta agtgaacttc tttatttctc
    37861 caaaaagatt gcaaacttcg ccacgacttc atacctttca ggtctcctcc taaatctaca
    37921 ataaggcgat gatttttgtc tatttgagaa agaatttcag gaatctcttt atcccacaag
    37981 ggggtatcag cgtacctccc ttttccctgg ggtaaaatag aaacttgact caaaaaacgg
    38041 tcacttcgag cagtaaaatc tcctttagta aaatctaaat ctaagagata gctacccatg
    38101 cccaaagatt gaaaaaaaga agttagattt agagcaatct ctgtttttcc caccccattg
    38161 ggaccggcca gaataaatat ctctttttct tccatctctt gagataaaaa actactgttt
    38221 ttaagtattt gcattttgag ctcttttcat caaagtttca gcgtactgaa gagccgaatt
    38281 atcttgaccc ccatctccca tcattcgagc taactcttta attctttctt cttcagaaag
    38341 agaggaaact tccacccagg tactttcttc atctcctcgc ttttccactt taaggtgatg
    38401 atgggcaaaa caggcaattt gaggtaaatg agtgatagag atgacttgat gtcctgtggc
    38461 aattcttctc aacttgtctc ctacccaaaa agcagtttga cctcctactc cctggtcaat
    38521 ttcatcaaaa actaaaatgg gaatccctct cacctctctg gtgagagatt ttatagctaa
    38581 aattattcga gatagctctc ccccggaagc tacctgctct accggaaaaa gaggttctcc
    38641 tggattcaag gaaatgagga aggaaacttc gtcatcttct tctttctttt ccagttctat
    38701 ctccagagcc actttagaaa aggaaagctc ttttagctct ttctccaatt tttctttgaa
    38761 tactttagct gctttctctc gatgatgaaa aagtttttcc tttgcctcca tcaaattctt
    38821 ctctttaatc tccagctccc tctcccattc ctctaattct tctaattccc ggtatagctg
    38881 agaaagctta atctttttct cctcaataaa actgattaaa tctttagtag tcagacaatg
    38941 gtattttctc ttccacctct cgatttctgc cacttccttt tctaactcct ctacttttaa
    39001 aggagaaaag tcaaaagagt ttctgacctt ctctacctga taaagcactt cctgtaactc
    39061 taactctatg tttttaagag tttcttctac agaaggagga aaaagagcta attcatcttt
    39121 tccttcccag tttcttctta ctcttctcaa cgaagataaa aatccttctt tttcctcggc
    39181 tccagaaata aggtatataa tttcctctgc tatctcttga tacctctgag aattaacaca
    39241 tctctggaat tgttcctcta cttcttcaat tttttcaaga gttaaaccca tcttttccat
    39301 ttcttctaac atttctctta tttcttctat ttctacttct tgtccccttt taagttcctg
    39361 gattttctct tttattttat tcctctcttc tttaagatgc aggtaatttt ctcgatcgag
    39421 ttttccctct tcatctaaaa aagaatctaa aatagccaat ttattctcag gagtgagaaa
    39481 gcggttgttt tccccttgtc cgtaaatatc tacaagatga gaagttactt ctttcaaagc
    39541 agccaaggtg acgcttctac cgttgatatg agccttactc tttccttctc tatttacctc
    39601 tcgagaaaga accatccccc cttttatttc ttcactccat cctttttcct ttaatttttc
    39661 ttgtaataaa ggagagaaag aaatatcaaa ccacccctcc acctgggcaa aatcacttcc
    39721 ctttcttatc cattcctctc tggggcgagc tcctaaaaga aaagaaaaag cattaatcaa
    39781 aagagattta cccgctccgg tttctccggt aaccacattg aagcccggtg caaaatttat
    39841 ttcttgataa tcaacaagaa cgaaattttt aattattaga gttcttaaca ttcttctact
    39901 tgccttcgaa tatccattcc ccaggacagt ttgctgcgca ataaagaata aaatggcctc
    39961 tttggaccga cacgaattaa atgtgctgaa aaattagacc gagcaacagt gatatgctcc
    40021 tccttctgta taagataacc tcgttgccca tctaaggtta ccatggttcc ctctttttcc
    40081 gattccagaa ctatttcaat agtagaagag ggatccacca ctatagaacg agcgtataaa
    40141 gtgtgagcac aaataggagt taccaccaaa agctccagag taggaagaag tactggtccg
    40201 ccggcagaaa gagagtaagc gctggagcca gtgggagtgg ctacaattaa cccatcggcg
    40261 gggaaagaag ctaagaattc cccatcaatg taggtggaaa gacgaatcat ctgagcaaaa
    40321 ggtcctcggt acaccactac atcattaaga gcaaaatcgg aatcggattt tccatctcga
    40381 tggctaatat ggcattgagc catcattcgc ttttctactt caaatcttcc tctaatcacc
    40441 tcctctgctg cctggaacat attttctaca ctcgcttcag ctaaaaaccc caaccctccc
    40501 atatcaatac ctaaaagagg aactcctgag ggagaataaa gccagctcgc agaaagaaaa
    40561 gtaccgtctc ctcccaaaac gaatactact tctggctttt cttctatttg cgtctcacct
    40621 tccacaataa gagtatttat cccctgagac cggagaaaat tgtccagctc ccgggcctta
    40681 tctcttactt tctgaatttt ctttttaatg aaaatatgtg ctgtacttaa aactttatcc
    40741 atcgtccaat tattaaacct aaaacaaaaa ataaaatact taaaattacc atccatctcc
    40801 agatagaagc gttactaaaa gggaaagtta ctcggtataa ctcatcactt tctccattca
    40861 ccagtataat tgctttaggg cggtaaacca gataatactc taacagctgt ctcctcaccc
    40921 ggggatggtc aaaatgcaaa tattcaccgg tttttacttc agctacatat aaatcttttc
    40981 ctcgacgcac aagataatct gctcgtgcct tacaaggaat tttcttacca tttacctgaa
    41041 taaaaaactc tctttctggc tgctcctcta ctaatttaaa accactattc tctaaaagag
    41101 aagcagcttc tttttccacc acctgagctc gggagaaaat tttctttctt cggtgtcttc
    41161 tccaagctga tgaaacaaac atagtaatca ctactgccac ggagaaggaa aatatcatcc
    41221 cccataccca gggattgaaa ttcatattat tattttcctt tactttggtg agcttcttcc
    41281 accacctggt ttattatact tcttaagctc cagtttgtta aatctgagct ccgttttacc
    41341 cagtatccta aaaactctat attgcctttt gctccccgaa taggggaaaa agttaaacct
    41401 tgaaaagaaa aacctgcttc ttgggcttga gttaaagttt cttctaaaac tcgttcatgc
    41461 acttttttat cttttactac tcctttttta acttcctgtc ggccagcctc aaattgaggt
    41521 ttaatcaagg caattaatag gccatcatct ttaagtatag atgcagcagg agaaaaaatc
    41581 aggcggagag aaatgaagga aacgtctata gtaacaccat ctactttctc tcctagaaaa
    41641 ctcaagtcaa gatagcgggc attaactttc tccttaacca ccacttgagg gtgatgacgg
    41701 agtttctcat gtaactggcc ataccccaca tcaatagcat aaacttttga tgctcccctt
    41761 tggataagac aatcggtaaa accaccggta gaagcgccaa tatcggcaaa gaccaaacct
    41821 tgaggattta tttgaaattc agttatcgct ttttctagtt tttctcctcc ccgactgaca
    41881 aatcgaggcg gctcctctat ttggataaaa gcaccttttt ctactaaagt agagggctta
    41941 agtagctgac ctaacccctc tactttcact ttgccactgc caattattct ctgtgctttt
    42001 tctcgactat cacataaacc actttttact aagagttcat ccaggcgaat tttctctttt
    42061 ttcatcctct aatattttct ttctattaca aatctggcta tttctaaaag gagaggaaag
    42121 gagcgaggaa tagataaaag aagatgaata gcttcttctt ctttattttt cattattttt
    42181 tcgctctctt tcactccctt aattttcacc agagtagctt tgtcctgtat ttcatcctta
    42241 cccggggttt tacctaaaat ctccttctct tgagtcgcgt ctagtatatc atctttaact
    42301 tgaaagcact ctcctaaaag catcccgaat ttttctaaaa cctctttttc ctgagaagtg
    42361 gcagaaccag ctatcgctcc gcatagaatt gaagcggtaa gaaaggaggc agtttttttg
    42421 cgatatattt cccagacctt ttcctcgctt acttcttttc cctctccttc taagtctaat
    42481 acctgtcctc ccaccattcc ctctatacct aaggctttca agataacatt taccatgtct
    42541 aatacttttt ctttaccaaa aaactcaacc aattgttcgt ttcccactat gagccgtaac
    42601 ccctctatca gaagagcatc tcctgctaaa actgccatag cttccccaaa agctcgatgc
    42661 atactgggtt ttccccgacg gtaatcatca ttatccatac agggaaggtc atcatgtacc
    42721 agagaaaaag catgtaccat ctctatagca ctggctacag gcagcaaatg ttctttttct
    42781 attccatagc tcaaacctgt agccagagcg agagatgcac gaattttctt ccctcccttt
    42841 accccataaa tgataggaga tactaaagaa gaagtaacac ttaagggaga aaagtaacgt
    42901 tctaaatatt catctactaa gtgagcattt tcttgaagaa aatcttctat tttcatgttt
    42961 tttctctttt aggaagataa ttttcccttt cttttaagtt tttcgctaat tttcctaata
    43021 ctccattaac aaactttccc gactcctctg ttccatattt ctttcccaac tccactgcct
    43081 cattaatagc cacacttact ggcacatcag gaagaaaaac tatctcaaat atggctaaac
    43141 gcagaatatt gcgatctaca ttagccatcc gataaagagg ccattcttct gaagcttgag
    43201 aaagtacctc gtcaatctcc tttttttt
//
LOCUS       DAOSVE010000004        42822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_622_length_42822_cov_68.616831, whole genome shotgun sequence.
ACCESSION   DAOSVE010000004 DAOSVE010000000
VERSION     DAOSVE010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 42822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42822
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_622_length_42822_cov_68.616831"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            344..1516
                     /locus_tag="PK016_00710"
     CDS             344..1516
                     /locus_tag="PK016_00710"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HXL01632.1"
                     /translation="MDNFEFLNPTKIVFGRDTENRVGQEIRKCQAKKVLLHYGGGSIK
                     RLGLYERVTSSLKESGINFIELGGVKPNPRVSLVREGIKLCRERGIDFILAVGGGSVI
                     DSAKAIGIGVPYDGDVWDFYTGKAEPQKTIPVGVVLTIPAAGSEASPGSVLTNEDGWF
                     KRAVNAEIMRPRFAILNPELTFTLPPYQTVCGASDIMAHVMERYFTPTRHVDLTDRLC
                     ESTLKTVIKNVPVVLEQPDNYDARAEIMWAGTIAHNDLLSTGRVGDWASHDIEHEISG
                     IYDVAHGAGLAVVFPAWMKYVYRENRDKFVQLAVRVWNVDMDFENPERTALTGIDRLE
                     KFYKSVGLPTSLKELGISDDRLEEMADKCTAGDTKTVGNFKKLGKQDVLNILKMAL"
     gene            2204..2671
                     /locus_tag="PK016_00715"
     CDS             2204..2671
                     /locus_tag="PK016_00715"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF014752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ERCC4 domain-containing protein"
                     /protein_id="HXL01633.1"
                     /translation="MDAREQYPWKFPECHVVRENLPVGDYGLKREDRIVALVERKTRE
                     NFLYNLQYFETLRASLHELKLVPFGAMVLECSYADLINPKKNPYFKPSYIADVLAELY
                     FLFPDIQIVFCDNRKIANEWTYRWFKRIYQFYEEQSSLRRKLKTAGGGKWVKL"
     gene            2851..4407
                     /gene="pckA"
                     /locus_tag="PK016_00720"
     CDS             2851..4407
                     /gene="pckA"
                     /locus_tag="PK016_00720"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HXL01634.1"
                     /translation="MQSLFYPLDKAGVRNSRCVHYNVVVPQLLEEAIKREEGILTSTG
                     AFNVLTGKYTGRSPQDRYIVDDEITHNSVDWGKINIPIEPEKFNRLHGKLCAYLQGRD
                     IFVYDGFVGVDPECRLAVRFINEYAYQTVFVENMFLEATKEELANFQPGFTVICAPNF
                     KADPGEDGVRSEAFVIINFQERMVIIGGTSYGGEIKKSIFTVMNFLLPQRGILPMHCS
                     ANVGERGDDVTIFFGLSGTGKTSLSADPRRRLIGDDEHGWSEKGVFNFERGCYAKCIN
                     LSREKEPQIWEALRYGAIMENVVVDPVTREADYNDARYTENTRTSYPIEYIPNALIPG
                     MAGHPKVVILLTADAFGVMPPVAKLTREKAVEFFLLGYTSKLAGTERGVVEPQATFST
                     CFGAPFMPLHPQVYAKMLGEKISQHGSRTYLVNTGWMGGPYGVGKRIDITYTRTIVHS
                     LIDGSIENYPFREDPIFGFLVPEEIKDVPRELLHPRTTWRNGSEFDEQAKKLQSDFEK
                     NISRFKLSSV"
     gene            4898..6073
                     /locus_tag="PK016_00725"
     CDS             4898..6073
                     /locus_tag="PK016_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL01635.1"
                     /translation="MKKQFLLIPFFFVLIVIGLGLSGMGAVIPEMAQHFSVSYSVVGR
                     VFLFHGLGYFVSILLAGILGDIINQVSILKLGIFLALAGFGGISFLPSFPLVMASFLL
                     MGVGLGFIDAMVNPVATSVFTEKPATTLNIIHAFFGLGSLSAPRLYSFLVAEGYGWRN
                     LYQICALFTLVTLVIFLFPFIPRKNQSSQFKELLGVFKQPALWVMGFTTLFYAGGVST
                     LNGWLVSYLEEGGVKRGEGAIFLSYFWLGIMLGRLILSSISDKIGHLNTIRLNALGGI
                     VFVSLFLLLPFHSASAFLLLFGAGFMLSTLIPTTVSYAIATFPEVTSTASSWVLFNNS
                     LGNFFFPWMGGIVGTVFNLRLTMIFVPLFLVLMFLFQQWLVKMGRKSAMVLGNYQKE"
     gene            6094..7437
                     /locus_tag="PK016_00730"
     CDS             6094..7437
                     /locus_tag="PK016_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01636.1"
                     /translation="MNDIQYWEEKLDSFDYEERWNVFSKLVEKAKKGEIIFPPPSPQV
                     NLHFHTFFSFNAYGYSPLHVIWKARELGLSMAGSVDFDVLDAVPEFQRGGLEVGLPFS
                     SGIETRIYLPEFPDKEFSSPHEPGVAYYMGNGFTRLPDEDSEAERILLNLKKIAQTRN
                     QKVIEKVNVYLDRVQIHYQQDVLPLTPSGNPTERHIVLAYALKSEALIPDEEERAEFW
                     GEKLSMGKGEIKKLFGDKATFYDFIRDKLIKFGGVGYVNPDSGDFPSRAELNRMTLES
                     GALPSFSWLDGTNSGERDPETLLEFCLANGIETLFLIPDRTWNIPDEEERKIKLAKLY
                     EIVEETKKRDMPVFVGTELNRYGQKLVDDFDSPYLKPLTPYFLESAWTLWGHVVMEMT
                     SRRGYTSDWAKENFSERHKKNQFFASLGKNTPAEEKAMERVGRKSTSHLLREFGG"
     gene            7579..7932
                     /locus_tag="PK016_00735"
     CDS             7579..7932
                     /locus_tag="PK016_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HXL01637.1"
                     /translation="MAEFLNPFSGLAPDKKMSLSELIRAIRLNIAAEEEATHLYTAHA
                     DATDHPLAKKVLLDIADEERVHAGEFLHLLQILTQDENQFLAEGKQEVEEMQEELRQE
                     GKTVEGEPPTVGPLK"
     gene            7953..8744
                     /locus_tag="PK016_00740"
     CDS             7953..8744
                     /locus_tag="PK016_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HXL01638.1"
                     /translation="MASKYLSREDAPLTPQIWELLDKTMIEVAKANLSGRRILPWEGP
                     FGLGLKFVSLSGISTEEGVSMSQALSLVEIQKSFRLRKMDLANYEIEGVTIDLTPLIE
                     ATRECARKEDEIIYQGLKGVPGLFTVRGSQKMKLSSWEEIGKAATDIIQAVTLLDEAG
                     FHGPYALALAPERYNLLLRIYNQGAKTELEHLKEIVGDGVVKAPFLKKGGVILDSNRN
                     YVSLVVGQDMTVGFIGPEEENLVFNISESMVPLIREPQAIGVLEE"
     gene            8957..9940
                     /locus_tag="PK016_00745"
     CDS             8957..9940
                     /locus_tag="PK016_00745"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXL01639.1"
                     /translation="MNYRVFGRTGWAASEIGFGSWAIGGGWGKVEEEEAIKALHTAID
                     QGVNFIDTADVYGDGRSEQLIARVLKERKERVYVATKAGRRLSPHLASGYNRENLNAF
                     VDRSLSNLGVETIDLLQLHCPPTEVYYNPEVFDILDEMVKTGKIRYYGVSVEKVEEGL
                     KALDYPGVRSVQIIFNMFRQRPAELFFRQCREKRVAVIARVPLASGLLTGKITKDTQF
                     PPDDHRNFNRFGQSFDRGETFAGVDMDKGLAAVEELKKIKPDGFTMAQFALKWILMFP
                     EVATVIPGAKNPEQARENSQVSELSPLQEEVMEKISEIYDKYIKKEVHYRW"
     gene            10003..10845
                     /locus_tag="PK016_00750"
     CDS             10003..10845
                     /locus_tag="PK016_00750"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HXL01640.1"
                     /translation="MKELEFQEIVQQVQKALEELNIISPELLREKMLKALATEDNDLA
                     REMLSQVMENYVVAEERHLPLCQDTGMVMAKVEKGEEVRIKGGSLRQALDEAIRRAYK
                     DNYFRKSIISDPFFGKNTGDNTPGVYFFELQEGDKLRIDLLVKGGGCDNISSLGILEP
                     GSKPEEVEKFILRELDRKGGQACPPLIVGVGIGGNAPYALYLASRALSRPLGEVNPDP
                     RYQELEEKWREDINRLGIGPQGVGGKTTCLEVRIETYPCHIASFPVGLVCNCHVFRQK
                     TLKW"
     gene            10851..11432
                     /locus_tag="PK016_00755"
     CDS             10851..11432
                     /locus_tag="PK016_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012459338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HXL01641.1"
                     /translation="MAERKEVILKTPLSVEEIKFLRAGDKVYLSGVIYVARDLTHQCL
                     IEDLEKGKELPFSLSGSVIYYAGPTPARRGEIIGSIGPTTSSRMDKYLEYFLQKGLRA
                     TIGKGERGEEVKKLLEKYGAIYLLACGGAGAYLSTFVIEKEVLAYEDLGAQALLRLKV
                     EQLPLLVAYDAQGGDIFEEGKIKYRNLALYNEY"
     gene            11453..12307
                     /locus_tag="PK016_00760"
     CDS             11453..12307
                     /locus_tag="PK016_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HXL01642.1"
                     /translation="MFWNIIFLLLIFSVYLPVLQRNIIEQQRMAAIKSLERKRRSRVI
                     AMIHRQESVSFLGLPIARYINIEDSEKILRAIHLTPKDMPIDLILHTPGGLVLAAQQI
                     ACAIKRHPAKVTVFIPHYAMSGGTLIALAADEIIMDPNAVLGPVDPQIGTAQAQYPAA
                     SILKALEEPNPNRDDQTLILGDIARKAINQVYQVVLDLVDEHLPQDKAQELATILSEG
                     RWTHDFPITLEMAQELGLPAKEGLPDEIYQLMDLYPQPQQQRPSVEYVPTPYTPHRRP
                     TGGKEKIE"
     gene            12322..14424
                     /locus_tag="PK016_00765"
     CDS             12322..14424
                     /locus_tag="PK016_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HXL01643.1"
                     /translation="MGKQLIITEKPSVAQDIARALGKMQKKKEYWENEDYIVTWAVGH
                     LVTLAEPEDYDKKLKMWRLALLPVVPDEFKLKAIEGAEERLKVIEKLIDSKEVEVVVN
                     ACDAGREGELIFRYIYEFLAGRKPVYRLWLSSMTQEAIKEAFSRLKPGNTYELLAQAA
                     KCRSEGDWLVGINGTRVFTARYKTLLSVGRVQTPTLAILVEREREIENFVPVPYWEIF
                     AEFSSPSGSYRGKWENKEDRVWEKEKAQSIVDKVKDRDGKVTEYKEHQVKEPHPLLYD
                     LTELQRDANKLFGYSAKRTLDSAQRLYERHKLITYPRTNSRYLSPDLISQVEKSWHML
                     AGCGFAEWVREDLSPEKALKDRRVFDGSKVSDHHAIIPTGEKIDWESLSENDKKIMDL
                     IVKRFIASFYPEAVWVHRSIKTVVEGEKFVSKSKVLLEAGWRAIYGKEEGEEFLPAVK
                     KGTAVKTQKVWIEEKETKAPPRYTEATLLSAMEGAGRFVEDEELQEILKDSGIGTPAT
                     RAAIIERLIEVGYVERNGKTLIPTPKGKELINLIENIPVPELSSPQLTGEWEKKLNQI
                     EKGEFSRDTFMKEIKKLTVEMVEKVKNMEDQGERERMSLPVGNCPLCGAPVYENRKSF
                     SCSRWKEGCPFTIWKKIAGRFINRQEAQKLLTTGRTSKLYGFKSRKGKKFSAYLVLQK
                     EGKIEFEFSTREVSSSVS"
     gene            14544..15803
                     /gene="murA"
                     /locus_tag="PK016_00770"
     CDS             14544..15803
                     /gene="murA"
                     /locus_tag="PK016_00770"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HXL01644.1"
                     /translation="MSKFVIRGSKPLHGEVRIGGAKNAALPILAATLLADSPSLIRNV
                     PNLLDVKTMLSVLESLGSKVEKVGEYAYQIFPTLYDISEAPYELIRKMRASFLVTGPL
                     IARHGRAQVPLPGGCAIGSRPIDLHLKGFSHLGVEISMHSGYIEAWASQLHEGEVYFD
                     FPSVGATENIMMLAATIEGETVISNAACEPEVVDLANFISLMGAEIEGAGTDTIVVRG
                     TKNLHGCEYTIINDRIEAGTFCVAAAITRGNIEIKGVEYSSLRSVITKLEEIGAVVER
                     KGEDSIFVALDSRPKAANIKTMPYPGFPTDMQAQFMALLSLAEGVSVITETVFESRFA
                     HVGELERMGARIRVEGRSAVVTGVEKLAGTQVTAPDLRAGAALVLAGLAAEGETEVYG
                     VLHIDRGYENLEIKLRSLGASIDRYDE"
     gene            15821..16834
                     /locus_tag="PK016_00775"
     CDS             15821..16834
                     /locus_tag="PK016_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HXL01645.1"
                     /translation="MWSKRLGIDLGTATTLIYVYGKGIVLNEPSVVAISKNTNEILAI
                     GREAWEMIGKTPEYIVAHRPLREGVISDYEVTRRMLTYFIQQVCGKSLFKPDVVVCIP
                     SGGTGVEKRAVLDAAYRAGARKAYLIEEPMAAALGAGIDITGPSGSMVIDIGGGTTDI
                     AVLSLGGVVASQSVRVAGDQLDEAITKHLRKKFNLLIGEKTAEVIKIEIGWAIPPKEE
                     KVLKVKGRDLVSGLPKEIALTSSEIYKCLSEQLSSIVEAARSVLEKTPPELAADIGER
                     GICLTGGGSLLRGIDEMLAKALSTPVYVAEDPISCVALGAGKVLDYLKLLRDGFLYTK
                     REK"
     gene            16841..18481
                     /locus_tag="PK016_00780"
     CDS             16841..18481
                     /locus_tag="PK016_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HXL01646.1"
                     /translation="MIGQSKKQCWFILILICFLLGLPLPGESASISYFPFSHIKRGME
                     AVGKTVFYGTEVEDFQLEVMDVVEGKKVEESYFVVKVTDKKLKEMGGISAGMSGSPVF
                     IRGKIAGALSYSWETKDNLVGVVTPIEAMLSLWEEETIQEEEILSPSSLLTPESTVFV
                     SGLGKRATERVANKLRERFSLKEIFSVPQLLWGRKENSEGDSLQPGSAIGIQLVKGDA
                     EVMSIGTLTLRDGDRILALGHPFLHRGKANYFLSSVYVNFSLQGTNLPFKVGKVIKEM
                     GVIDQDRSAGVAGRIGIMPEVSEVVIKVRNEGGEEREYSFEVVRDEDILIDMLPELVL
                     DAIDRSIDSQIPGSANVKFNLEGEDFSWQEEFFWISDTDIASTTSSGSGEVFKTILGN
                     PCRELSLDRIEMEIDVTSGIQRAWLTSLDLSKVIERDKEIEGKINLFLWREGEREVSF
                     SFLVPADFLPGTAEISVRGKSNGNLKLETSKEEVIFSPSLYDYLQKQLDSLHSEGLTV
                     EIFSKAGPSSQNKRDYFTQWVNLPLILEGSVSQEVWLR"
     gene            18491..22081
                     /locus_tag="PK016_00785"
     CDS             18491..22081
                     /locus_tag="PK016_00785"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HXL01647.1"
                     /translation="MGSPGLGFFLTFGLIWSYGQRRITGYFTTRIISELEKTTGSKIN
                     IGSFDFRFPNRLQLWEVRVKGKDFSFFTPRIVLQWNWRSLFDTSSSEEAFRLSVEEVE
                     AFSSENFLQKLPFLSLPFLPPGEIKIKKIKFSQFSGNEVHLKKDREGKLSFEALFPSG
                     KVEGVFQERESLSLNFSLIQNNLPFQGEVYWNMKDGKVEGKISSPRTLSFETVLSFTG
                     EDVILSFLELWEGEKTKLLFRGEGKINNHLFPLEGKGEVYLGEEKHFLDVEMNKDSSS
                     SLWKGKFSIRGERANIKGEVSSSQEEVLAFNIEPGLTFYGVTVEEEIQAQASWKEGEA
                     VITIPATAVKFDISPELKGEGVFQGIINEEEQEWKAHLTWKGGYLQWNDFLVNEPRVD
                     IEAGKDGEIVLQGEGSWGEGKIILSGRGFKDRFSLQGNFQGIPIEQLIEGTDFPLKGW
                     ISGEWERREDGLIFLSLKEGEAFWEDWRLGSIEKGEVDFSPDALLIHDLCLRQGKGVI
                     SGDFEKREDNWEGKIGVKDYPVSYSLEDEKIELALGGDIKVKGKDLLLSLFSSSWEIG
                     KIKGEKLSLVGKVENGEIEVEDFSCDWGGGYLSLKGKVNPYREVNLQGEIRNLEIPSY
                     QGLEGHLERVFLYLSGPWEKVAFLLEGEGKNFFWQGEPWGDYFHLLLRGDVPLPQKGE
                     SPLLADYLNPYLLEEGTIEVRGINLEQLMRKVSSYPVSGMTDIVINLDTQNNLWFFHS
                     EDFSFAVQDFHFRGKVEGNYDGEDLVINKLSFQELEGGLEIGGSLKWGGKTKTLAGEI
                     EGKVGRSFSIPYGVLQLRGEANISLSGDEEKPLWQGEVKGEGEIWQGGKKYASFSGVQ
                     GIIKGEEVQLKSGNLHIAGLDWAVKGVISREESEVHLSGGGKLEIPGENIGLSRIESD
                     IVLQYCDEKISLQGEAKIFDGWGDFTKGEDTGDYSFLLSQLEDKLEELPLSLELTLST
                     GNKVRVKTRFLDLEMEGKLKIREENGKINGEGKLEVVEGVYNLMGRKIPLKGYITFSP
                     LYGLNPQISLQGEEEVGGYHIFLEATGLMDSYKLTLKSEPFLKEEEILSLLFLGGKDA
                     YLSLNNLDWQSLLWKIGQSLLGGNFFSRSRFFDGLEIKFSSSDDSDFYGLRWEKSIGE
                     NCFIGYTQDLSGEGNSSWNFKINFDKEWSLKMEGDTEGEINWMLEFNTKF"
     gene            22102..23781
                     /locus_tag="PK016_00790"
     CDS             22102..23781
                     /locus_tag="PK016_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HXL01648.1"
                     /translation="MVQKKIKKLLGGLVFLVVLGMGGMVYAQTVSPPVVAIRVEGNEA
                     INDQLILSAVSTSLLEPYNPEKVQADVQAIYDLGYFSKVWVETKQYPDGIELIFKVQE
                     FPPVKEIRISGNTALSYDEIRKVMILSLGQIMNWKIFQHDLERIKALYSDNGFLITAV
                     EEINFSEEGVISFTIKEGMVEAVEFEGLEKTKEHVLRRELTFELPMVFDFAKIQENLR
                     TIYNLGLFENVSMKLEPGSDRDHVVVKVQVKEKLTGEAGLGVGYNSEKGWLGYVRYQE
                     SNLGGNAQRAELRYEFGARTLYRLLFEEPWLFEEPIYFNLAIYDQIEEKKEWVNHEVV
                     GKYEEERIGGYITLGGKIKEDWGWRLRYKSEDIDINVLEGNPPDGGGKTNSLTPIVFY
                     DTRDVLDNPHGGWYGTLQAELAGRFLGGEHNYNKYTLDLRNYIETGENTVLALRFLGG
                     IADTELPSYEKFSVGGVNTLRGYDLFEFEGDKMLVFNLEYRWEVSEGTQLVFFGDTGN
                     AWPLSQGIRWDDIKIGYGVGVRFDTPIGPVRLDYGIGESGGQTYFSIGQTF"
     gene            23817..24335
                     /locus_tag="PK016_00795"
     CDS             23817..24335
                     /locus_tag="PK016_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HXL01649.1"
                     /translation="MRRSSFLVGLTLMAVMILMIAIPSWAAEPAVGLVDLNKVLDAHP
                     NTQKILDAENRLREEMQKRQTELNEKGKGKTMEEVQKLEEEMNAEWAPVRDQLLKERQ
                     DLVNQRYGDVIAAIKKVAESQKLTLVVRSELRIPVSQSELMEMPIVLYGGLDITDQVI
                     EEIKNMTATPEK"
     gene            24342..25364
                     /gene="lpxD"
                     /locus_tag="PK016_00800"
     CDS             24342..25364
                     /gene="lpxD"
                     /locus_tag="PK016_00800"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HXL01650.1"
                     /translation="MKLQEIAKLVGGSLIGDPHWEVKGIKEPGEAEENDLILFYHAPY
                     LEKVLSSASKAVITKPGMERELKDKNIILVDNPRYAFSKLVPYFFPSYFPERGVHPQA
                     FVHPQAFLGTNVSVGPFTVIEEGVTVGDNSIIFSQVYLGKNTQVGRECVFYPQVVVRE
                     DCWIGDRVILHSGVVIGADGFGYEKVGNSYYKIPQVGKVVIEEDVEIGANSTVDRAAL
                     GVTRIGAGSKIDDLVMIAHNVKIGKNVVIVAQSGVAGSTEVGDEVVMGAQSGIIDHRR
                     VGKGVQMAARAVVTTNIPDGVIVSGFPAQEHKKELREKALIRKLPELWEKVRNLEKMV
                     SKQVEK"
     gene            25361..26188
                     /locus_tag="PK016_00805"
     CDS             25361..26188
                     /locus_tag="PK016_00805"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HXL01651.1"
                     /translation="MSSVVLQRTLASSCTVEGVGLHSGKPVRVTLFPASENSGIFFRA
                     GETIIPAAIQYVEVTPRCTTLKKGKKQISTVEHFLAACWGSEIDNLEAVVEGEELPGG
                     DGSSIFWFNVFRKVGFQIQSSPRKYLIVKENIKVGENRHYLFAFPDEEFRVWYFLDGG
                     ENGHFSQALEFSENDDSDKLLSARTFAFSREVAEILQKGWGKGIREKAFILNEEGESS
                     IPLRFPGEAAGHKILDLMGDLMLTGKRVKGCFIGIRSGHALNREMVKRITEASSEEN"
     gene            26175..26999
                     /gene="lpxA"
                     /locus_tag="PK016_00810"
     CDS             26175..26999
                     /gene="lpxA"
                     /locus_tag="PK016_00810"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HXL01652.1"
                     /translation="MKKIDPTAKIEKEVEIGEEVEIGPYSILQGEIKVGAHTKIGAFT
                     VMEGKVKVGPYCNIGHHVVIGTPPQDLSYRGEETQVVIGDRTTLREFVTVHRATGEGK
                     ITSIGDDCLIMAYCHIAHNCRLGNGITMANSSALAGYVEVEDLATLSGWVGVHQFVRL
                     GKLVMIGGLSKVVMDIPPYTLADGHPARIFGLNVVGMKRRGIEKDKREEIHQIYRLIY
                     HNNLPRSQALQELKRSSFDPQLVEEIVGFWEKSKRGVARWVKGRKDSDEEDTSPSW"
     gene            26971..27750
                     /gene="lpxI"
                     /locus_tag="PK016_00815"
     CDS             26971..27750
                     /gene="lpxI"
                     /locus_tag="PK016_00815"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF017989.2"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HXL01653.1"
                     /translation="MKRILLLAGEGDIPLLIYEKAKKEGEEVLVLSSLSFSLPEELKP
                     DLWIDGISLTQILKVIREEKITHLCLAGKIPKNLIFEEDRFQEEFKKLFSAPKLRDGV
                     LLQAALEKLKEEVDIISPLFFMKEYLTPSGKIAGRDPSQEEWEDISYGARIARFLADE
                     EIGQTVVVKRGAVLALEGAEGTDETIKRGVSLGKEVVVVKMARSQQSFLIDIPAIGEK
                     TVQCLKNGGIIALEAGKTFLLDREKIGEEAEKWGVGVIGIN"
     gene            27751..28848
                     /gene="lpxB"
                     /locus_tag="PK016_00820"
     CDS             27751..28848
                     /gene="lpxB"
                     /locus_tag="PK016_00820"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HXL01654.1"
                     /translation="MSKLFISIGDASGDYYGGEIIYLLRQRNKEIEFLALGGERSQKA
                     GARLLYPTVSLSTVGLWEALFTLREWKKVWTLSQEVIEKEHPDVVLLLDNPGFNLRLA
                     SLCQEHKIPVIYFVPPQVWAWGRKRASFLSQHADWILTIFPWEVDYFQGGKAKVKWVG
                     HPATHLIPPARFSPLEEKKKIALLPGSRKREIVNYLAVIKEFIPLLLGKGIVEFTAIA
                     ASKEAQEILVKEMAGFPVTIKNRRELLSVLSGSSLAISSSGTVTLEVALAGIPQIIVY
                     RVALLTFLVARLVMGKTLIGLPNIVMGKEISPELIGKDFSTSRLLKEVEKILNNPQAR
                     EKALENSLKIREKLNRGNPFEEVVQVVKEYL"
     gene            28889..29950
                     /locus_tag="PK016_00825"
     CDS             28889..29950
                     /locus_tag="PK016_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01655.1"
                     /translation="MYYWLYPLLCDLSRQPSPPRVWVYVPPCQFSTGREEEVVKNYPL
                     VDKVFSPRETVSFCLRKGKFSSPGLILYLGGDLFYAVLLKRRTGFPLWVYCGYSKWLN
                     KIDLYLARFWGDFEKLDFDRKIFLGDLLFSSISTLPSSVFPPGSPRFLFLPGSKKVFY
                     PYLLPFFFQVGKELKRVYPEVSLALSFPDHYNFNDIPELKTIPPFFSPFFGKTSSLMA
                     EADLIVTIPGSNNLEIAYRGKRGIVILPFWGLEKIPLEGPMLLLEKIPFWGKIIKRNV
                     VKKMAGTRKWISLPNELLDKEVVKELRGNLSVSEVLEAIQEMLNKEPEIYFPEERFPR
                     NASPRLAEMIGEWWDGKAK"
     gene            29934..31673
                     /locus_tag="PK016_00830"
     CDS             29934..31673
                     /locus_tag="PK016_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL01656.1"
                     /translation="MEKQNSAFNRIWRYLRPYKNYVIGAFLMALFGAGFNLAIPWLVK
                     GTIDNALVGKDNRLLLLISLGVVVLFFFKGLFSYLQNLWISYSGFRAITCLRSELYQH
                     LYFLPASFFRDNPAGEVVSRLTNDVAMLQNLFSNAFVNLFMDFFIFSGSIILLFFIHW
                     KLALFSVAVLPLVGFSVDYLGKRIKGVSHLLQKRVAALTSLIERAVAGMKVFQSYMTG
                     NYETEKFEMENEANFALAMKQAQAKALLTPLVELVSACGVIAVLLYGGREVIQGHLTA
                     GGLIAFLGYLVTAASPLSRFSSSFQIFQQSLASAERLFDFLDLPLWGREEEGEKEITS
                     LQEGIRFKNVSFSYQEEKVLKNFNLEIKKGEKVGIVGHSGAGKTTLINLLLRFYDPEE
                     GSIEIDGIDIRELTFSSLRNLMGVVLQDTLVLGGTIKENILYGNLDATLEEVRLASVR
                     AHAHEFIVHLEKGYDTEVGEGGARFSGGQKQRIAIARALLKDPPILIFDEATSNLDPE
                     SERYILDTIANVGKDKIVIVVAHSLSMVKDLDRIILVEDGEVKGDGTHEELLQSSFYY
                     RRLFGIENFDKVL"
     gene            complement(31618..32163)
                     /locus_tag="PK016_00835"
     CDS             complement(31618..32163)
                     /locus_tag="PK016_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HXL01657.1"
                     /translation="MGEIKNPQPVKLFIAVLYEERNILENILPQIEKYWGEIEWISEE
                     IPFNYTSYYRNIGENLKRVFIVFKSLIDPEELADIKVITNEIEKATKEGEKERRINLD
                     PGYFDGGKIVLASTKNFSHRVYLRQGIYAEATIKWEKGDFRPFPYTYPDYQSPEYRPS
                     LQKIRELYQNSLCQKDADNRS"
     gene            32346..32930
                     /locus_tag="PK016_00840"
     CDS             32346..32930
                     /locus_tag="PK016_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HXL01658.1"
                     /translation="MTEEKEMLKEPEEINKNREMEESGLSEIEELHSLIEEQKKIIEE
                     KEKIIKDYINDLKRLKADFDNFRKRETLFRQDFVKKANRGLIFELLPILDDMERAIRE
                     AKAREVDEAIVQGIELIYCKFLNILEKEGVKPIETEGQKFDPKYHEAIATVSLPDYDD
                     YEIAEEIRRGYLYQDEVLRPAQVRVNRISKDFMA"
     gene            32946..34859
                     /gene="dnaK"
                     /locus_tag="PK016_00845"
     CDS             32946..34859
                     /gene="dnaK"
                     /locus_tag="PK016_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HXL01659.1"
                     /translation="MAKVIGIDLGTTNSVMAYLEGGQPVIIPNKEGSRLTPSVVAFTK
                     NGEILIGQLAKRQAITNPKNTIYSIKRLMGRRYDDPEVEKARKVLPYEIVPGEHGEVC
                     VKVGDRVYTSPEISAMILRKMKEDAEEYLGEKITEAVITVPAYFNDSQRQATKDAGKI
                     AGLDVKRIINEPTAAALAYGLGKGKEEVIAVYDLGGGTFDISILEIGEGVFEVKSTSG
                     DTFLGGDDFDRRVMDYIIDEFRKDQGIDLRGDQMALQRLKEAAEKAKCELSTALQTEI
                     NLPFITADASGPKHLVMTLTRAKLEQLTMDLIEKTIGPCERALKDAGLKPEDIDAVVL
                     VGGMTRMPKVQEVVERIFKKEPRRGVNPDEVVAVGAAIQAGVLKGEVKDVLLLDVTPL
                     SLGIETLGGVFTKIIERNTTIPVSKSQIFTTAADNQTTVEVHVLQGERPMARDNVSLG
                     RFQLTGIPPAPRGVPQIEVKFDIDADGILHVSAKDLATNKEQTITIKASSGLSKEDID
                     RMIREAELHAEEDRRRQEEVEVRNQADNLVYTVEKNLRESGDKLSSGVRSQVESALNA
                     LKEAMNKDDIADIRRKMDDLQNASHAMAQELYQKAAQSETSTSDQTGSSGGATQDENV
                     VDADYRVVDDDTK"
     gene            complement(34960..35790)
                     /locus_tag="PK016_00850"
     CDS             complement(34960..35790)
                     /locus_tag="PK016_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltetrahydrofolate cobalamin
                     methyltransferase"
                     /protein_id="HXL01660.1"
                     /translation="MVIIGEKINGTIPDVQKAIEERNENFIRDLARRQVEAGADYIDV
                     CAGTTSDVEFEILKWLMDIVQDTIDKPLCIDSPNPAIIEQVFNYAHQPGIINSVSGEG
                     SKCEIIYPLIQESQWQVIALTCDSRGIPADVQTRIDIAKSLVEKAQKYDIKPEQIYID
                     PLVVALATDNQSVRKFIETLKAIKQLYPDIKTTSGLSNISFGMPLRKIINHAFLTVAI
                     FAGMDSAIMDPCNKETRTALLAAEAIAGKDKYCRNFLNAYRSGLIGPSKDQSSFSSAQ
                     "
     gene            36096..36725
                     /locus_tag="PK016_00855"
     CDS             36096..36725
                     /locus_tag="PK016_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777418.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HXL01661.1"
                     /translation="MQEELFNKIVNMDEEGSIKLAKEYLDKGGKPQELLEVCRRAMDA
                     IGEKFERGEYFLSELLLGGEIFKSIMEFVLPLIEESETTRLGTIILGTVKDDIHNIGK
                     DIFKAFAQAAGFEVIDLGVDVPEEKFVEAVKEHKPEIVGMSCLITAGISSMKRTREAL
                     RASGLENVKIIIGGGRVDESVKQFVGADAWSDDAARGVRICKELIGVEG"
     gene            36732..37958
                     /locus_tag="PK016_00860"
     CDS             36732..37958
                     /locus_tag="PK016_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXL01662.1"
                     /translation="MAKSPEELAKERLARIEAAINLKEPDKVPLWGVGGDIVAAYAGM
                     TQHEFCYDYDKAVKAIEKFMRDFPFDTSMAGLMGLEGRIFCVAFSEFPDISPALSLIT
                     GPMHDVLGDKYYRYPGREIDENATPQFIGGTFMEPGEYDRLIEDPVGFVAETILPRAC
                     TNLDTPRKAMATWVRLGMECSKFFSAAAEIGRVSAELGYPPFPMGWGYSPLDIIGDFL
                     RGVGTVVLDIYRYPDKVKKATEALTEPIANYALYHKYMGAKYAMIPLHLNEYLSPKLY
                     NEFYWPYLKAVILKLYEEGIKSMVFFEGHHDAHLDTILELPEGWGIAYFEKTDVVKAK
                     KVLQGHTCVMGGIPMGLLVGGTPMKIEEYIKNLLEEVKPGGGFILAPGVGTAPSETPI
                     ENIHALIEAVEKYGYY"
     gene            38499..39665
                     /locus_tag="PK016_00865"
     CDS             38499..39665
                     /locus_tag="PK016_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL01663.1"
                     /translation="MESKKTVAIAIMSVFFIAMGVGTITPAIQDIAEAFPHLSFSTIL
                     LVFTLPSLFIIPSSILAGVAAGSKIKYRTLLIWGTLLFSLAGVAPIFMSNFTTILVAR
                     ALFGIGLGIIAALGNALVLAFYEGQERANMLGLGGVMMNIGGIVLQLLGGFFSSIRWN
                     LAFLPHLIGLITLIMVTFMLPEPEKREEIIAKKVTLPWSVYLIAIIFGVITMLNYPTG
                     ANMSTIIITGNLGDAASAGIVLSLFTVGGMVAGAVFGKLYQKIALFSIPVGFAVMAAG
                     AGLVYYANSLSILALGMAFIGMGFGWLMPAFMMMVGIVTSPEAYATASGILMAFMNLG
                     GFLSTYYIAMLLRVNSAIRFPIFVEMVALFIGTLIYLILSGSIHRSLLRDEPTA"
     gene            39968..41734
                     /locus_tag="PK016_00870"
     CDS             39968..41734
                     /locus_tag="PK016_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014904247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HXL01664.1"
                     /translation="MNRYKVTILPDDVLLLVTEGTFLKEVFIRSGIDFEFPCGGMGLC
                     KRCKVKIIKGDGKKEDAFACQLKVESDLMVEISSREERHTILTEGVERRVVVDPLVRK
                     VYLELPLPSLKDNRDDWARLVGKEGIKPDLPVIQDLPEKLRKSNFKVTAVVGDHKIIS
                     VEGGDTSNKLLGMAFDIGTTTVVGYLINLKTGEEITKVSSLNSQTKFGADVISRITFA
                     SDTPLGLDKLHREIINKLNNLIGEAVNTTGYEAENIYTIVVVGNTTMHHLFLKIQPGY
                     LARSPYVPAVTEPMVVDASDINLRINLAGKVFTFPNVAGFVGGDLVADALASEMDKAK
                     KLKLLVDIGTNGEIMLGKEGKYLACSAAAGPAFEGAQISCGMRGASGAIAYVSMDEGD
                     CKYTVIGETLPRGITGSGLVDIIAELLKIGMVNKKGSMLESDNIKSELGKEYKDRIIS
                     IDGILAFVVAENTATGQPVYINQKDIREFQLAKGAIAAGIEILLKTYGAKVEDIDEVF
                     LAGAFGNYLRPESACRVGLIPQELEGRIKGIGNAAGIGAKLAMLSKEEYERAIVLSRK
                     THYIELSSHPEFERTFLNKLDF"
     gene            41864..42514
                     /locus_tag="PK016_00875"
     CDS             41864..42514
                     /locus_tag="PK016_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HXL01665.1"
                     /translation="MKNIVIACQTIKDELTLAIKETQCEYPVIWIDSSYHLYPGKLRL
                     KLQEEINNLKDFDNILLAYGFCGNAIAGLKASSANLVFPKTDDCISIVLSKDGELRQM
                     KTAYFLTRGWIESPGGIMVDYSNALKRYGEEKAKRMFSLMLEHYDKLVVIDTGAYNIV
                     DYIEKIQKFAKISGLDLVVEKGGIGLLKKLLTGPYDDKFCIIEKGQEVNIDDILLS"
BASE COUNT        12777 a         6814 c        10410 g        12821 t
ORIGIN      
        1 taatatttta accagttttg cgggatacta tagtcttaaa aattatgacc atcttccttt
       61 ttattttgta tttatgaatg tcgggagaga tgttttttct cccaaggtcg ggatgcattt
      121 cctggtatta ttttagtttt ctgtctttta tacttttaat cctctctgga gactactttg
      181 ctataacttt tagaggggac atgattaaat gataaaaata gtactttctc tctttcttgc
      241 tatcataatg ttctccacct actttattat ggggctttgg cgctccttga ctgaataagg
      301 gtttttgtta ttaaatatag taaaaattaa gaaggagaaa aatatggata attttgagtt
      361 tttgaatcct actaagatag tttttggtag agatacagaa aatcgggtgg gtcaggaaat
      421 tagaaaatgc caggctaaaa aggtgttgct tcactacggt ggtggtagca taaaacgctt
      481 gggactttac gagagggtga cctcttcact caaagagtcg ggtattaatt ttatagagct
      541 tggcggtgta aagcccaacc cccgggtgag tctggtgcgt gaaggtataa agctgtgccg
      601 ggaacgtggt atagatttta ttctggcagt gggtggtggg agtgttatag attctgctaa
      661 agccataggt ataggagtac catacgatgg tgatgtgtgg gatttctata ctggaaaagc
      721 tgaacctcaa aaaacaatac ctgtgggtgt ggtattgact ataccggcgg caggaagtga
      781 ggcgagtccg gggtcggttt taaccaatga agatggatgg tttaaaaggg cagttaatgc
      841 agagattatg aggcccaggt ttgctatatt aaacccggag ttaactttta ctctaccacc
      901 ttatcagaca gtgtgtgggg cttcagatat aatggctcat gtaatggagc gttattttac
      961 tcctacccgt cacgtcgacc ttaccgatag gctatgtgag agcacactta aaacagttat
     1021 aaaaaacgtg cccgtagtac ttgaacaacc tgataattat gatgcccggg ccgaaataat
     1081 gtgggctggc acaatagccc ataacgacct tttgagtacc ggccgagtag gtgattgggc
     1141 atcgcatgac atagaacatg aaattagtgg tatatatgat gtagctcacg gtgccggatt
     1201 agcagtggta tttccggcat ggatgaaata tgtatatcga gaaaacaggg acaaatttgt
     1261 tcagttggca gtgcgggtat ggaatgtaga tatggatttt gaaaacccgg agcgcacagc
     1321 attgaccggg atagataggc tggaaaagtt ttataaaagt gtgggacttc ctacttcgtt
     1381 aaaggaatta ggaatatcgg atgaccggtt agaagagatg gctgataagt gcacagctgg
     1441 tgatacaaaa actgtgggca attttaagaa attaggaaaa caagacgtat tgaatatttt
     1501 gaagatggca ttgtaagtca gtaaagtagg aatagtaccc ggccttattt actttgattg
     1561 cggatggcct ttgtcagggt ggtgtattta tatttataac tgacagaaga aaagaagaat
     1621 aatttaaggt tagaggagct tacatttttt aagaaaaagc tggttgttag taaaaattat
     1681 ggggcgggat gaaaatccgc aggctggaag aaattcgggt gatgggaaat gaagtctctt
     1741 tttatttata tatttttaat caggagaaat tgatactgtg aaaccacctc ttcgctggtt
     1801 ggtctttgcc aataacagtg atccaaagtt tctctatatt ttggatattg aggaaacgct
     1861 agggtgtttt ctatgcttga gtgcacagga caaatggccg ggtacaaata aaaaaatatt
     1921 ttgctagcga ttgggctatt ttgtcgagga tgatttagaa aaatttaatc tggtggatgc
     1981 ctctactatc aaatttttat ggcattacgg gaaaaaagct ggaaatcatc ttagaccgga
     2041 aggtaagaaa acgttgctgg tttattatga ttgaaaaaag tataaaagtc gtcctggtga
     2101 atattatgaa caaatattct ggattaccca gacttcagcc cgggcgagag ggagaaaagc
     2161 atatatccct caaggaggga aaaatgaatc atttgatatt attattgatg ctcgagaaca
     2221 atatccctgg aagtttccgg agtgccatgt agtaagagaa aatctcccgg ttggcgatta
     2281 tggtttaaag cgggaagaca ggattgtggc cctggtagag agaaaaacca gagaaaattt
     2341 tctttataat ctgcagtatt tcgaaactct cagagcttcc cttcatgagc tcaagctcgt
     2401 tccttttgga gctatggttc tggagtgttc ttatgcggat ttaatcaacc ctaaaaagaa
     2461 tccttatttt aaaccttctt atattgctga tgttctggct gaactatatt tcctctttcc
     2521 cgatattcaa atcgtttttt gtgataaccg taaaattgcc aatgaatgga cctatcgatg
     2581 gtttaagaga atttatcaat tttatgaaga acagagcagt ctgcgacgta aactaaaaac
     2641 cgccggaggg gggaaatggg taaaactata acacttaaga agaagcactc cagtttcact
     2701 ttgagccttc tgtgtcgaga tgttttaccc ggacaggatc catgagggag ctatctttat
     2761 tacctgtttt actcttaaaa ccttttccga ctttactttt ttctcgattg tggtaaaatt
     2821 ctggaaattt aaataaggga ggaaaggtaa atgcaatctc tcttttatcc tctggataag
     2881 gctggtgttc gtaattctcg ttgtgttcac tataatgtgg ttgttcccca gcttttagaa
     2941 gaagctatta aaagagaaga aggtatttta acttctactg gagcttttaa tgtgttaacc
     3001 ggcaaatata ctggacgttc tccccaggac cgttatattg tagatgatga aattacccat
     3061 aattctgttg attggggtaa aataaacatt cccatagaac cagaaaaatt taatcgactt
     3121 catggtaaac tgtgtgctta tcttcaaggt agagatattt ttgtttatga tggttttgta
     3181 ggagtagacc ccgaatgtag attagcagta cgttttatca atgaatatgc ttatcagaca
     3241 gtttttgtag aaaatatgtt tcttgaggca accaaagaag agttagccaa ctttcaacca
     3301 ggatttacag taatttgtgc tcccaatttt aaagctgacc ccggagaaga tggtgtgcgt
     3361 tctgaagctt ttgttatcat caattttcag gaaaggatgg taatcattgg tggtacttct
     3421 tatggaggag agattaagaa atccatcttc acagtaatga atttccttct tccccagcga
     3481 ggaattcttc ccatgcattg ttcggctaat gtaggagaga gaggggatga tgtgactatc
     3541 ttttttggac tttccggtac gggaaaaact tctctttctg ctgaccccag gcggcgcttg
     3601 attggagatg atgaacatgg ctggtcggaa aagggagttt ttaattttga gagagggtgt
     3661 tacgctaagt gtataaatct ctctcgagag aaagagcccc agatctggga agctttgcgc
     3721 tatggggcta ttatggaaaa tgtagtggta gaccctgtta ctcgagaagc agactataat
     3781 gatgctcgct atacagaaaa cactcgaacc agctacccca ttgaatacat tcccaatgcc
     3841 ctcattcctg gtatggctgg acatcctaag gtggtgattt tattaactgc tgatgctttt
     3901 ggggtgatgc cccccgtagc taagcttacc agagaaaagg cggtagagtt tttcttactg
     3961 ggctatacca gtaagttagc aggaactgaa agaggtgttg ttgaacctca ggccactttt
     4021 tcgacctgtt ttggtgctcc ttttatgcct cttcatcctc aagtgtacgc caaaatgtta
     4081 ggagaaaaaa tatctcagca tggttctcga acctacctgg ttaatactgg ttggatggga
     4141 ggtccttatg gggtaggtaa gcggatagat ataacttata cccgaactat tgttcattct
     4201 ctgatcgatg gtagcattga aaattacccc ttccgagaag accctatatt tggtttttta
     4261 gttcctgaag aaattaagga tgttccccgg gaattgcttc atcctcgcac tacctggagg
     4321 aacggaagcg aatttgacga gcaagctaaa aaattgcaga gcgacttcga aaaaaatatc
     4381 agtcgcttca aactctcttc ggtttaaggt aaactcaaaa actgctatta ctttaagctc
     4441 accagctaca gctggtgagc tttttattta gatatgggtt attataagta aggtcggtac
     4501 cctcttctcg ttgttctgaa gttatcctat agtatcccgc aaaacctgtt aaactactaa
     4561 acctttaaaa aacaaattca tcggaagtta aaaatggaat cgccaaacac agagggatac
     4621 tataatcctt agtagtcttt tacttttatg tttttgtttc tcctgttttt cttcattttt
     4681 cgggggttat aaaggggttt ttttctctca tagttcggtt ataaaggggc agagcccctt
     4741 tatagtatat attttcgaaa gttgttgtgg ttaacaaaat attaagctat attagttcgg
     4801 aaaataggag taaaggtaaa ggcagatgcg agaatcagat ttcaaagtag tataccgaat
     4861 gtgtaattga aaaatagttt gaggaagtga atgaaaattg aaaaagcagt ttttgttgat
     4921 tcctttcttt tttgttctca tagttattgg tttaggtctc tcaggtatgg gggcagtaat
     4981 cccggaaatg gctcaacact tctctgtctc ctactctgta gtgggaagag ttttcttatt
     5041 ccatggttta ggttactttg tttctattct tcttgctgga attttaggag atattattaa
     5101 tcaggtttct attctgaagc tgggaatttt cctggcttta gccggctttg ggggaatttc
     5161 ctttcttccc tcctttcctt tagtcatggc ttcctttttg cttatgggag tggggttagg
     5221 atttattgat gctatggtta atcctgtggc taccagtgtt tttactgaaa aacctgctac
     5281 taccttaaat atcatccatg ctttttttgg gttagggtct ttgtctgctc ctcgtcttta
     5341 ttccttttta gtggcggaag gctatggttg gcgaaatctt taccagatat gcgctctctt
     5401 tactctggta actttggtta tttttctttt tccttttatt cctcggaaaa atcagtcttc
     5461 tcaatttaag gaattactgg gggtatttaa acagccagcg ttatgggtaa tgggtttcac
     5521 cactcttttt tatgctggag gagtgagtac tttgaatggt tggctagttt cttacctgga
     5581 agaaggagga gtaaaaagag gagagggagc tatttttctc tcttacttct ggttgggtat
     5641 aatgttaggg agacttatcc tttcttccat ttcggataaa attggacatc tcaacaccat
     5701 tcgccttaat gccttaggag ggatagtgtt tgtttctttg tttctgcttt tgccttttca
     5761 ttccgcttct gcttttcttt tgctttttgg cgctggtttt atgctctcta ctttgatacc
     5821 caccactgtt tcttatgcta ttgctacttt tccggaagtg acttctactg cctccagctg
     5881 ggttttattt aataatagct tgggtaattt ctttttcccc tggatgggag gaatagtggg
     5941 gacggttttt aacctacgtc taactatgat ttttgttccc ctgtttcttg ttttaatgtt
     6001 tttatttcag cagtggttag ttaaaatggg tagaaagagt gctatggttt tgggtaacta
     6061 tcaaaaagaa taatggaaat gaggtaataa gatgtgaacg atattcaata ctgggaagaa
     6121 aaactggata gttttgacta tgaagagcgc tggaatgttt tttctaagtt ggtggaaaaa
     6181 gcgaaaaaag gagagataat ctttccccct ccttctcccc aagttaatct tcattttcat
     6241 accttttttt cctttaatgc ttatggttat tctcctcttc acgttatctg gaaagctaga
     6301 gaacttggtt taagtatggc cgggagcgtg gattttgatg tgcttgatgc agtacctgag
     6361 tttcaacggg gagggctgga agtaggttta cctttttctt cggggattga gacccggatt
     6421 tatcttcccg agtttcccga taaagaattc agttctcctc atgaaccagg ggtggcctac
     6481 tatatgggta atggttttac tcgtcttccc gatgaagata gtgaggcgga gcgaattctt
     6541 ttaaatctga aaaaaattgc tcagaccaga aaccagaaag ttatagaaaa agttaatgtt
     6601 tatctggata gagtgcagat ccactaccaa caggatgtat tgcctcttac cccctcgggt
     6661 aaccccaccg aaaggcatat agtccttgct tacgccctga aaagcgaagc tcttatccct
     6721 gacgaagaag aaagagcaga gttctgggga gagaaattaa gtatgggaaa aggagaaatt
     6781 aaaaaacttt tcggggataa agccaccttt tatgatttta tccgcgataa gctcattaag
     6841 tttgggggag tgggttatgt gaatcctgat agcggagatt ttccttctcg agcggagttg
     6901 aatagaatga ctttagagtc aggagctcta ccttcttttt cctggttaga tgggactaat
     6961 agtggagaaa gagacccgga gactcttttg gagttctgtc tggctaatgg tatagagact
     7021 ctttttctca tccccgatag aacctggaat atccccgacg aggaagaaag aaagataaag
     7081 ctggctaagc tttatgaaat agtggaagaa acgaaaaaaa gagatatgcc tgtttttgtt
     7141 ggaacagagc ttaaccggta cggacaaaaa ttggttgatg actttgattc tccttatctt
     7201 aaacctctta ctccttattt tttggagagt gcctggactc tgtgggggca tgtagttatg
     7261 gagatgactt cccggagagg ctatacgagc gattgggcta aggagaattt ctctgagcga
     7321 cacaagaaaa accagttttt tgcctctttg ggtaaaaata cccctgctga agagaaagca
     7381 atggaaaggg tggggagaaa atccacttct cacttacttc gagaatttgg gggatagtta
     7441 ttgattatgg cacttactcc tgataccagg gtctggaatg tctatagaat agatagaaga
     7501 gataaaagaa agtgagatag agaaagattt aacctccttg tggtaaaata aactaaaagc
     7561 ttttaaggag gtaagaaaat ggctgaattt ttaaatccct tcagtggttt agctcccgat
     7621 aagaaaatga gtttatcaga gctcatccgg gctatccgtc ttaatatagc agcagaagaa
     7681 gaagctactc atctttatac cgctcatgct gatgctactg accatcctct ggctaagaaa
     7741 gtattgctcg atatcgctga tgaagaacgg gttcacgccg gagagttttt gcatttactg
     7801 caaatcctta cccaggatga aaatcagttc ctggctgaag gaaaacaaga ggtggaggag
     7861 atgcaagaag aacttcgcca ggaaggaaaa actgtagagg gagaacctcc taccgtgggg
     7921 cctctcaaat agagttgaaa ggaggataaa aaatggctag taaatacctg agccgggaag
     7981 atgctccttt aactcctcaa atttgggaat tgctggataa aaccatgata gaagtggcta
     8041 aagctaatct ttccggaaga cggatccttc cctgggaagg tccttttggc ctggggctga
     8101 aatttgtctc tttatctggc atctcaacgg aagagggagt ttcaatgagc caggctcttt
     8161 ctctggtgga gattcagaaa tctttccggt tgagaaaaat ggatctggct aattatgaga
     8221 tagaaggagt aactatagat ttgacacctc tcattgaggc tacccgggaa tgcgctcgta
     8281 aagaagatga gattatttat caagggctga aaggcgttcc tggtcttttt actgtcaggg
     8341 gaagtcaaaa aatgaaatta tcttcctggg aggaaatagg taaggcagca actgatataa
     8401 ttcaggctgt tacccttctt gatgaagcag gatttcatgg cccttatgct ctggctctgg
     8461 caccggagcg ctacaatctc cttttgcgga tttataatca aggagccaaa acggagctgg
     8521 aacacttgaa ggagatagtg ggagatggag tggtgaaagc tcctttcctc aaaaaaggag
     8581 gagtaatatt agattctaat aggaattatg tttctttagt ggtgggtcag gatatgactg
     8641 tagggtttat cggacccgag gaagaaaatt tggttttcaa catttctgag agtatggttc
     8701 ctctcatccg ggagcctcaa gctattggtg ttttggaaga gtaaacgaaa cggccaatcc
     8761 aggattatag attttccaga gaggagcaat tgcccctctc tggaatagag ttattttatg
     8821 aaatatttag tgagttttgg ccgatcggga atttttaaca tcttttattc ttttactttt
     8881 tgtttataga ttaatttact ttgttctgac ttgctttcgg ttataatttc taaaaagcga
     8941 tgggggaggg agaattttga attatcgggt ttttggtcga actggttggg cagcgagtga
     9001 aattggtttt ggctcctggg ctattggagg tggatgggga aaagtagaag aggaagaagc
     9061 tattaaagca ttgcacactg ctatagacca gggagtcaac tttattgata ctgcagatgt
     9121 ttacggggat ggaagaagtg agcaacttat tgctcgagtt ttaaaagagc gcaaagaaag
     9181 agtatacgta gccactaaag cgggtaggag actttctcct caccttgctt caggctataa
     9241 tcgggaaaat ttgaatgctt ttgtggatag aagtctctcc aacttaggcg tagaaactat
     9301 agaccttttg cagctccatt gcccccctac tgaagtttat tataaccctg aagtttttga
     9361 tatactggac gaaatggtta aaacaggtaa aatccgctat tatggagtga gtgtagaaaa
     9421 ggtagaagag gggcttaaag ctctcgatta cccgggagtg cggagtgtgc aaatcatttt
     9481 taacatgttt cgtcaacggc cagcagagct tttctttcgc cagtgtcggg aaaaaagagt
     9541 agcagttata gctcgagttc ctttagccag cgggctttta accgggaaaa taactaaaga
     9601 tacccaattt cctcccgatg accaccgtaa ttttaaccgt tttggtcaat cttttgaccg
     9661 gggagaaact tttgctggag tagatatgga taaaggttta gcggcggtag aagaactgaa
     9721 aaaaattaag cccgatggct ttaccatggc tcagtttgct cttaagtgga ttctcatgtt
     9781 tcctgaagtt gctacagtta ttcctggagc taaaaatcca gagcaagccc gggaaaattc
     9841 tcaagtctcg gagctttctc ctcttcagga agaggttatg gagaaaatat cagagattta
     9901 cgataaatat attaaaaaag aagttcacta ccgatggtag agtaaaggat tagaacggag
     9961 tctccggggt tttaaaatta agctgcgaag aggaagtaga gcatgaagga gttagaattt
    10021 caagagatag ttcaacaggt acaaaaagca ctggaggagc tgaatatcat cagtcctgaa
    10081 ctacttaggg agaaaatgct aaaagcttta gctactgaag ataatgattt agctcgagaa
    10141 atgttatctc aggtgatgga aaattacgtg gtagcagaag agcgacatct tcctctctgt
    10201 caggatacgg gtatggtaat ggccaaagtg gaaaaagggg aagaagtaag aattaaagga
    10261 ggcagtttac gtcaggcact ggatgaggct ataagaaggg cttataagga taattatttt
    10321 cgaaagtcca taatatctga ccctttcttt ggtaaaaata ctggagataa taccccagga
    10381 gtctattttt ttgagcttca agaaggggat aaacttcgta tcgaccttct ggttaaaggt
    10441 ggagggtgtg ataacataag ctctctggga attttagagc cgggtagtaa gccagaagag
    10501 gtagagaagt ttatcctcag ggaactggat agaaagggag gacaggcttg tcctcctctt
    10561 attgtaggag taggaatagg aggtaatgct ccttacgctc tctatcttgc ttctcgagct
    10621 ctatctcgtc ctctgggaga agttaatcct gaccctcgct atcaggaatt agaagaaaaa
    10681 tggcgagaag atattaaccg tctagggatc ggtcctcaag gagtaggagg gaaaactacc
    10741 tgtctcgaag ttagaataga aacttatccc tgtcatatag ccagttttcc agtgggttta
    10801 gtgtgtaatt gtcacgtttt tcgtcagaaa accttgaagt ggtgagataa atggcagaaa
    10861 ggaaggaagt gattttaaaa actcctcttt cggtggaaga gattaaattt ttgcgggccg
    10921 gtgataaggt ttacttgagt ggtgttattt atgtggctcg agatttaact catcaatgcc
    10981 tgatagagga cctggaaaaa ggtaaagaac ttcctttttc tctttcaggg tcggttattt
    11041 attacgctgg tcccacccct gctcgtcggg gggaaataat agggtcgatt gggcctacca
    11101 ccagtagccg gatggacaaa tatctggaat actttctgca aaaagggcta cgagctacca
    11161 ttggtaaggg ggaaagggga gaggaagtta aaaaactttt agaaaaatat ggggccatct
    11221 atttattggc ttgtggaggg gcaggagctt atctttctac ttttgtaata gaaaaagaag
    11281 tactggctta cgaagatttg ggagctcaag ctcttttgcg cttgaaagtg gagcagcttc
    11341 cccttttggt agcttatgat gctcaagggg gagatatttt tgaagagggg aaaataaaat
    11401 atcgtaatct tgctttatat aatgaatatt gacattaagg aggtttttaa aaatgttctg
    11461 gaatataatt tttcttctcc tcatttttag cgtttactta cctgttttac aaagaaatat
    11521 tattgagcag cagagaatgg cagcgattaa gagtctggag agaaaaagaa ggtcacgagt
    11581 aattgccatg attcacagac aagaatcagt tagtttttta ggtttaccta tagcacgcta
    11641 tattaatatt gaggattcag aaaaaatctt acgggctatt catctcaccc ctaaggatat
    11701 gcccattgat ttgattctcc acactccagg gggattggtg ctcgctgctc agcagattgc
    11761 ctgtgctata aaaagacacc cggctaaagt taccgtgttt attcctcatt atgctatgtc
    11821 aggaggaaca cttattgctc tagcagcaga tgaaataatt atggatccca atgctgtttt
    11881 aggacctgtt gaccctcaaa taggtacagc tcaggctcaa tatccggctg cctcaatttt
    11941 aaaagctctg gaagagccta atcctaaccg ggatgaccag accctgattt taggtgatat
    12001 tgctcgtaaa gctattaatc aggtttacca ggtggtttta gatttagttg atgaacatct
    12061 tccccaagat aaagctcagg aattagccac tattttatcc gagggtcgat ggactcacga
    12121 ttttcccatt actttagaga tggcacaaga attgggtctt ccggcgaaag agggtttacc
    12181 agatgaaatt taccagctta tggaccttta ccctcagcct cagcagcagc gaccttcagt
    12241 agagtatgtt cctactcctt atactcctca tcgtagacct acaggaggaa aagaaaaaat
    12301 tgagtaaagg atgtgaatgt tatgggtaaa cagctaatta ttactgaaaa gcccagtgta
    12361 gctcaggata tagcccgtgc tctgggtaaa atgcagaaaa agaaagaata ctgggagaat
    12421 gaagattata ttgttacctg ggcagttggg catttagtaa ctttagctga accagaagat
    12481 tatgataaaa agctaaaaat gtggcgtctt gcccttttgc ctgttgtgcc agatgagttt
    12541 aagctgaaag ccatagaagg agcagaagaa agattaaaag ttattgaaaa gctcattgat
    12601 tctaaagaag tagaagtggt agtgaatgct tgtgatgcag gtagagaggg agagttaatc
    12661 ttccgctata tttacgaatt tttggccggt cgcaaaccag tttatcgcct ctggctttct
    12721 tctatgactc aagaggccat aaaagaagct ttttctcgtc ttaaaccggg aaatacctat
    12781 gaacttttag ctcaggcagc taaatgtcgt tccgaaggag attggttagt gggtatcaat
    12841 ggcacgcggg tatttactgc tcgctataaa actctgcttt ctgtgggaag agtgcaaact
    12901 cctactttag ctattctggt ggagagagaa agggaaatag agaattttgt tcctgttcct
    12961 tactgggaaa tatttgctga attttcttcc ccctctggtt cttatcgggg gaaatgggaa
    13021 aacaaagaag accgggtgtg ggaaaaagaa aaggcccaat caatagtaga taaagttaaa
    13081 gaccgagacg gaaaagtaac tgaatacaaa gaacatcaag ttaaagagcc tcatcctctt
    13141 ttatatgacc ttactgaatt gcagagagat gccaataaac tttttggcta ttcggctaaa
    13201 cgtactctgg atagcgctca gagactttat gagcgtcata aattgataac ttatcctcgc
    13261 accaactctc gttatctctc tcctgattta atttctcagg tagaaaaaag ctggcatatg
    13321 ttagcaggat gcggttttgc agaatgggta agagaagatt tatcacctga gaaagcctta
    13381 aaggatagaa gagtatttga tggaagcaaa gtttccgacc atcatgccat tatccctact
    13441 ggagagaaaa ttgactggga aagtttgagc gaaaatgata aaaaaatcat ggatctaatt
    13501 gtgaaaagat ttatcgcttc tttttatccc gaggcagtct gggttcaccg cagtatcaaa
    13561 accgtggtag agggtgaaaa atttgtcagt aaatctaaag ttcttctgga agcaggctgg
    13621 agagcaattt atggaaaaga agaaggagaa gagtttttac ctgcagttaa gaagggaact
    13681 gcagtaaaaa ctcaaaaagt ctggatagaa gaaaaggaaa ccaaagctcc tcctcgctac
    13741 actgaggcta ctcttttatc ggctatggag ggagcaggcc gctttgtaga ggatgaagaa
    13801 ctccaggaaa tattaaaaga tagtggtatt ggtactccag ccactcgagc agccataatt
    13861 gagcggctaa tagaagtagg ttatgtggaa agaaatggta aaactcttat acctactcct
    13921 aaagggaaag agctaataaa ccttatagaa aatataccgg ttcctgaatt atcgtctccc
    13981 cagcttaccg gggaatggga aaagaagctc aatcagattg aaaaagggga attttctcgg
    14041 gatactttta tgaaagagat aaaaaaatta accgtggaga tggtagaaaa agttaaaaac
    14101 atggaagacc agggggaaag agaaaggatg tctttacctg tcgggaattg tcccctctgt
    14161 ggagctccgg tttatgaaaa tcgcaaatcc ttcagttgtt cccgatggaa agagggttgc
    14221 ccatttacca tctggaaaaa gatagcggga agatttatta accgtcagga ggctcagaaa
    14281 ttacttacta cgggtagaac tagtaagctt tacggtttta agtctcgtaa aggtaaaaaa
    14341 ttctctgctt atctcgttct tcagaaggaa ggaaaaatag aatttgaatt ttctacccgg
    14401 gaagtttctt ctagcgtttc ttgatgtttt gaagggattt tttagaaacc gggatgtaga
    14461 tgtcagaaaa atcgattata atagtgcagg taccggtgtg gtgctttcac tgattatatg
    14521 agaggtagaa agagagaaaa cagttgagta agtttgtaat ccggggttca aaacctttgc
    14581 acggggaagt acggatagga ggagctaaaa atgctgcctt acctatttta gcagcaactt
    14641 tattagctga ttctccttct ttaattagaa acgttcctaa tcttttggat gtaaaaacca
    14701 tgctttcggt tctggagagt ttagggtcta aagtagaaaa ggtaggagaa tatgcctacc
    14761 agattttccc taccttatat gatatcagtg aagctcccta tgagttgatt cgtaagatga
    14821 gagcttcttt tctggtaacc ggtcctctta ttgcccgtca tggtcgggct caggttcctc
    14881 ttccaggagg atgtgctatt ggttctcgtc ccattgacct tcatcttaaa ggttttagcc
    14941 atttaggagt agaaatatct atgcattccg gttatataga agcctgggct tcccagcttc
    15001 atgaaggaga agtttacttc gattttccca gtgtgggagc aacagaaaac atcatgatgc
    15061 tggctgctac tattgaggga gaaacggtta tatctaacgc agcctgtgaa ccggaagtgg
    15121 tggatctggc taattttatt tctttgatgg gagcagaaat tgaaggagca ggtactgata
    15181 ctatagtagt gagaggtact aaaaatttgc atggatgtga atatactatt attaatgata
    15241 gaattgaagc gggtactttt tgtgtggcgg cagctatcac cagaggtaac atagaaataa
    15301 aaggggtaga atattcttct cttcgttcag taattactaa attagaagaa attggggcag
    15361 tggtagagag aaagggagaa gatagtatct ttgtggcctt agatagtagg cctaaagctg
    15421 ctaacattaa aactatgcct tatcctggtt tccctaccga tatgcaagcc cagtttatgg
    15481 ctcttctttc tttagctgaa ggggtaagtg tgattactga aacggttttt gagagccgtt
    15541 ttgctcatgt gggagagttg gagagaatgg gagctagaat tcgagtagaa ggaagaagcg
    15601 cggtggttac tggagtggaa aaattagctg gaactcaagt tactgctcct gaccttcgag
    15661 ctggagcggc cttggttctg gctggtttag ctgctgaagg agaaactgaa gtgtatggag
    15721 ttttgcatat cgaccggggt tatgaaaatt tagaaatcaa acttcgctct ttaggagctt
    15781 ctatagacag gtatgatgaa taaaacggga ggaattattt atgtggagta agagattggg
    15841 gatagacctg ggtactgcta ccactttgat ttacgtttac ggaaaaggta tagttttgaa
    15901 tgaaccgtcg gtggtagcca tatccaaaaa tactaacgag attctggcta tcggtagaga
    15961 agcctgggaa atgatcggta aaactcctga atatatagta gctcatcgtc ctcttcggga
    16021 aggagtaatc agtgattatg aagttacccg gaggatgcta acttatttca ttcaacaggt
    16081 gtgtggcaag agtctcttta aacctgatgt agtagtgtgt attccttcag gaggaacggg
    16141 agtagagaaa agagccgtat tagatgctgc ctatcgggca ggagcaagga aagcttatct
    16201 tatagaagag cctatggctg ctgctttagg tgcgggtata gatatcactg gtccttccgg
    16261 gagtatggtc attgacattg gtggaggtac tactgatatt gctgttcttt ccttaggagg
    16321 ggtggtggct agccagtcag taagggtggc tggagaccaa ttagatgagg ctataactaa
    16381 acatctgcgg aagaaattta acctcctgat aggagaaaaa acggcagaag ttattaaaat
    16441 tgaaattggt tgggcaattc cccctaaaga agaaaaggtc ctcaaagtta agggccggga
    16501 tttagtttcc ggtttaccta aggagattgc tctcacttct tcagaaatat ataaatgttt
    16561 gagtgaacag ctttcttcca tagtagaagc agctcggtca gtgttggaaa aaacccctcc
    16621 tgaattagct gctgatattg gagaaagagg tatttgctta accggaggag gatcactact
    16681 gcgagggata gatgaaatgt tagctaaagc cttgagcacc ccagtatatg tggcagaaga
    16741 ccccatttcc tgtgtggctc tgggagcagg taaggtatta gattatttaa aattgttaag
    16801 agatggattc ctttatacga aaagggagaa gtagccaggg ttgattggtc agtctaagaa
    16861 acaatgctgg tttattctga ttttaatctg ttttcttctg ggattacctt taccgggaga
    16921 aagtgcctct atttcttatt tccccttttc tcacattaaa agaggcatgg aggcggtagg
    16981 gaaaactgtt ttctatggta ccgaagttga agattttcaa ttagaagtaa tggatgtagt
    17041 agaagggaaa aaagtagaag aaagctattt tgtggttaaa gtaacggata agaaacttaa
    17101 ggaaatgggt ggaatatcgg cgggaatgag tggttctccg gtttttatcc ggggtaagat
    17161 agctggagct ctttcttata gctgggagac caaagataac ttggtgggtg tggtgacacc
    17221 tatagaagct atgctctctt tatgggagga agaaactatt caggaggaag aaattctttc
    17281 tccttcttct cttcttactc ctgagtctac tgtttttgtt tcgggtttgg ggaagagagc
    17341 cactgagagg gtggctaaca agttgagaga acgattctct ttaaaagaaa tttttagcgt
    17401 tcctcaactt ttgtggggta ggaaagaaaa ttccgagggt gattccttgc aacctggaag
    17461 tgctatcggg attcaattag tgaaaggaga tgctgaggta atgagtatcg ggactcttac
    17521 cttacgtgat ggagaccgga tattagcttt agggcatccc tttttgcatc gggggaaggc
    17581 caattatttt ctttcctcgg tttatgtgaa cttcagttta caaggaacga accttccttt
    17641 taaggtggga aaggtgataa aagagatggg agtgattgac caggaccgga gtgctggagt
    17701 agcaggcagg ataggaataa tgcctgaggt gagcgaagtt gtaattaagg ttagaaatga
    17761 aggtggggag gaaagagaat atagttttga agtagtgaga gatgaagata ttttgataga
    17821 tatgctaccc gagctggttt tagatgctat agaccggagt atagactctc aaatacctgg
    17881 cagtgcaaat gtaaagttca acttagaagg agaagatttt tcttggcagg aagagttttt
    17941 ctggataagc gatacagata ttgccagtac tacctccagc ggttcaggag aggtattcaa
    18001 aactattctg ggtaatcctt gtcgagagct cagtttggac aggatagaga tggaaataga
    18061 cgtaacttcc ggcattcagc gtgcctggct tacctctttg gatctctcta aggttattga
    18121 gcgggataaa gaaatagagg ggaaaataaa tttatttctc tggcgggaag gagaaagaga
    18181 ggttagcttt tcttttctgg taccagcaga tttcttgccc ggtacagcag aaatcagtgt
    18241 tagaggtaaa agcaacggta acctgaaatt agaaaccagt aaagaagagg taatcttttc
    18301 cccttctctt tatgattact tacagaagca gctggatagc cttcatagcg aagggttaac
    18361 agtagaaata ttcagcaaag cgggtccttc ctcacaaaat aagagagatt acttcaccca
    18421 gtgggtaaat cttcccttaa ttttagaagg aagtgtttct caggaagtgt ggcttagata
    18481 agttgaaaaa gtgggttctc ctgggcttgg ttttttttta actttcggtc ttatctggtc
    18541 ttatggccag agaaggataa ctggctactt tactacccgg attatttctg agttagagaa
    18601 aacaacagga agcaaaataa atattggaag ttttgatttt cgtttcccta accgacttca
    18661 gttatgggaa gtaagggtga aagggaagga tttttctttt ttcactcccc gaattgtttt
    18721 gcagtggaac tggcgttctt tatttgatac ttcatcttct gaggaagcct ttcgtttatc
    18781 agtggaagaa gtagaagcat tttcttctga gaattttctt caaaaattac cttttctttc
    18841 tctccctttt cttcctccag gggagataaa aatcaaaaaa ataaaatttt ctcagttttc
    18901 cggaaatgag gttcatttga agaaagatag agaaggtaaa ttatcttttg aagctctttt
    18961 tccctctgga aaagtagagg gtgtttttca ggaaagggag agtttatctc ttaattttag
    19021 cctgatacag aataacttgc cttttcaggg agaagtgtac tggaatatga aagatggcaa
    19081 ggtagagggg aaaatttctt ccccccggac tttaagtttc gaaacggttc tttcttttac
    19141 cggggaagat gtaattcttt cttttctgga attatgggag ggagaaaaaa caaaactcct
    19201 ttttcgggga gagggaaaaa taaacaatca tctttttccc ctggagggaa agggtgaagt
    19261 ttatctgggg gaagaaaaac attttttaga tgtagaaatg aataaagata gctcatcttc
    19321 tctctggaaa gggaaatttt ctatccgggg agagagggca aatataaaag gtgaggtttc
    19381 ctcctctcaa gaggaagttt tagcttttaa tattgagccg gggcttactt tttacggggt
    19441 gacggtagaa gaggaaatcc aggcccaggc ttcgtggaaa gaaggggaag ctgtaattac
    19501 tatacctgct actgcggtga aatttgatat ctctccagag ttgaaaggag agggagtttt
    19561 tcaagggata ataaacgaag aggagcaaga atggaaagct catttaacct ggaagggtgg
    19621 ttatctccag tggaatgatt ttttggtgaa tgaaccccgg gtagatatag aagcaggaaa
    19681 agatggagaa atagtgttgc agggtgaggg gagctgggga gagggaaaaa taatattatc
    19741 tggacgaggt ttcaaggacc gcttttctct ccagggtaat tttcagggaa taccaataga
    19801 gcaactgata gagggaacgg attttcccct gaaaggttgg atttctggag aatgggaaag
    19861 aagagaagat gggttaattt ttctctctct gaaagaggga gaagccttct gggaagattg
    19921 gcggttagga agcatagaaa aaggagaggt cgacttttct cctgatgctc tgcttattca
    19981 cgacttatgt cttcggcaag ggaagggagt aatctctgga gattttgaaa aaagggaaga
    20041 caattgggaa gggaaaatag gggtaaaaga ttatcctgtt tcttattctc tggaagatga
    20101 aaaaatagag ttagcattgg gaggtgatat aaaagtaaag gggaaagact tgcttttgtc
    20161 ccttttttct tcttcctggg aaatagggaa aataaagggt gagaagctct ctttggtggg
    20221 aaaagtagaa aatggggaaa tagaggtaga agatttttcc tgtgattggg ggggaggtta
    20281 cctttcttta aaaggtaaag ttaatcctta tcgggaagtt aatcttcagg gagaaattcg
    20341 aaatctggaa ataccttctt atcagggtct agaagggcat ctggaaaggg tttttcttta
    20401 tctctccggt ccctgggaaa aagttgcttt tcttctggaa ggagagggta agaatttttt
    20461 ctggcaagga gaaccctggg gagattattt tcatcttttg cttcggggtg atgttcctct
    20521 tcctcaaaaa ggtgagagcc cacttcttgc tgactattta aacccttatc ttcttgaaga
    20581 gggaacaata gaggtgaggg ggataaattt ggagcaacta atgaggaaag tttcctctta
    20641 tcctgtttcg ggaatgactg atatagtgat taacttggat acccaaaata acctgtggtt
    20701 tttccactct gaggatttct ctttcgcagt acaggatttt cattttcggg gaaaagtgga
    20761 aggaaactat gatggagaag atttagttat taataagtta tctttccagg aattggaagg
    20821 gggtttagaa atagggggaa gtttaaaatg gggaggaaag acaaaaactc tggctggaga
    20881 aatagaaggg aaagtaggtc gctccttttc tattccttat ggggtgctac aattgagggg
    20941 agaagccaat atttctcttt ctggtgatga ggaaaaacct ctctggcaag gagaagtgaa
    21001 aggagagggt gaaatttggc aaggaggtaa gaagtatgct agttttagcg gggtgcaagg
    21061 aataataaag ggagaagagg ttcagctcaa gagtggaaac ttacacattg ccggtcttga
    21121 ttgggcagta aagggagtta tttctcgaga ggagtctgaa gttcatcttt caggaggggg
    21181 aaaattagaa ataccagggg agaatatagg gctctccaga attgagagcg atattgtact
    21241 ccaatactgt gatgagaaaa taagcctcca gggagaagca aaaattttcg atgggtgggg
    21301 agattttacc aaaggagagg ataccgggga ttactctttt cttctttctc agctggaaga
    21361 taaattagaa gaattgccac tttctctgga acttaccctt tctactggta ataaagtgag
    21421 agtaaaaact cgctttttag atctggaaat ggaagggaag ctcaaaatcc gggaagagaa
    21481 tgggaaaata aacggggaag gtaagttaga agtggtagaa ggagtctata atttaatggg
    21541 aagaaaaatt ccccttaaag ggtatataac ttttagtccc ctttatgggc ttaaccctca
    21601 aataagtttg caaggagagg aagaggtggg aggatatcat atcttcctgg aagctacagg
    21661 tctcatggat agttataagt taactttaaa atcggaaccc ttccttaaag aggaggaaat
    21721 tctttctttg ctctttttgg ggggaaaaga tgcctatctt tctctgaata accttgactg
    21781 gcaatctctt ctctggaaaa tagggcaatc tttactggga gggaattttt tctctaggag
    21841 tagatttttt gatggattag agataaaatt ttccagttca gatgattctg atttttatgg
    21901 tttaagatgg gagaaaagta ttggtgaaaa ttgctttata ggttatactc aagatttgag
    21961 tggtgaggga aattcttctt ggaatttcaa gataaacttt gataaggaat ggtctctgaa
    22021 aatggaagga gatactgaag gagaaattaa ctggatgttg gaatttaaca ctaaattttg
    22081 acaatgtggg agggttgtga attggttcag aaaaaaatta aaaaattact aggggggtta
    22141 gtatttctgg tagtactggg aatgggagga atggtttatg ctcaaactgt ttctccacca
    22201 gtggtggcta tccgggtaga aggaaacgag gctattaatg atcaattgat tttatccgct
    22261 gtatctactt ctcttctgga accttataat cccgaaaaag tgcaggcaga cgttcaggct
    22321 atttatgatc tgggatattt ctctaaagtg tgggtagaaa ccaagcagta tcctgatggc
    22381 atagagctaa tttttaaagt gcaagagttt cccccagtta aggaaattcg catttctggt
    22441 aacacagcct tgagttatga tgaaatcaga aaagttatga ttttatctct aggccagatt
    22501 atgaactgga agatatttca acatgactta gagcgtataa aagctcttta tagtgataat
    22561 ggttttttaa ttacagcagt agaagaaatc aatttcagcg aagaaggggt tataagcttt
    22621 actattaaag aagggatggt agaagcggtg gagtttgaag gtttagaaaa aacaaaagaa
    22681 cacgttttac gtcgagaatt gacctttgag cttcccatgg tttttgactt tgctaaaatt
    22741 caggagaatt tgagaactat ttataatctg ggcctttttg agaatgtatc tatgaaattg
    22801 gagccgggca gtgaccggga ccatgtagtg gtgaaagttc aggtgaaaga aaaattaact
    22861 ggtgaggcag gattgggagt gggatataac agtgaaaaag gctggttagg ctatgttcgt
    22921 tatcaggagt ctaatttagg aggcaatgcg cagagggcag agttaaggta tgaattcggt
    22981 gcccggactc tttaccgtct cttatttgaa gagccctggc tttttgaaga gcccatttat
    23041 ttcaatctgg ctatctatga ccagatagaa gagaaaaaag agtgggtaaa ccatgaagtg
    23101 gttggtaaat atgaggaaga aagaatagga ggatatatta ctttaggagg taaaataaaa
    23161 gaagattggg gatggcgact gcgctataaa tctgaagata tagacattaa tgttttagag
    23221 ggtaatcctc ccgatggggg aggaaaaacc aattctttaa ctcccatagt gttttatgat
    23281 actcgagatg tattggataa ccctcatgga ggttggtatg gaacgttgca ggcagaatta
    23341 gcagggaggt tcttgggtgg tgaacataat tataataagt atactttaga tttgagaaat
    23401 tatattgaga cgggagaaaa tacagtttta gctttgcgct ttttaggggg tatagctgat
    23461 acagagttac ccagttacga gaaattttcc gtaggaggag ttaatactct gcgaggttat
    23521 gatttgttcg agtttgaagg agacaagatg ttggttttta atctggagta ccgctgggaa
    23581 gtttcggaag ggactcaact ggtctttttt ggtgatacag ggaatgcctg gcctctttct
    23641 cagggtatac gctgggatga tattaaaatt ggttatggag taggggtgag atttgatact
    23701 cctattgggc cagtgcggct ggattatggg attggagaat ctggcggaca aacctacttc
    23761 agtatcgggc agacttttta agaaaattta aaacatcata gaaaggaaga agaataatga
    23821 gaagaagtag ttttttggtc gggttaactt tgatggcagt aatgattttg atgattgcta
    23881 ttcctagttg ggcagcagaa cctgcggtag gcttagtaga tctcaataaa gttcttgatg
    23941 ctcatcctaa tactcagaag atattagatg ctgagaatag actccgggaa gagatgcaga
    24001 aaaggcaaac cgaattgaat gaaaaaggaa aaggtaaaac aatggaagag gtgcagaaat
    24061 tagaagaaga aatgaacgct gaatgggctc cggttagaga ccagctcttg aaggaacggc
    24121 aggatttagt gaaccagaga tatggtgatg taattgcagc aattaaaaaa gtagctgaat
    24181 ctcaaaaact taccctggtc gtccggagtg aattgcgtat acctgtttct cagagcgaac
    24241 tgatggaaat gcccattgtt ctctatgggg gattagacat cactgatcag gtgatagaag
    24301 aaatcaaaaa tatgactgct actccggaga aatagataat tttgaaatta caagaaattg
    24361 ctaagttagt ggggggaagc ttaataggtg acccccactg ggaagtaaaa ggcataaaag
    24421 aaccaggaga ggcagaggag aatgatttaa ttctttttta tcatgctcct tatttagaaa
    24481 aagtactctc ttcggcttct aaagcagtga taaccaagcc gggaatggaa agagaattaa
    24541 aagataagaa tattatcctg gtggataatc ctcgttacgc tttttctaag ttagttcctt
    24601 actttttccc ttcctatttt ccggagaggg gagttcaccc tcaagctttt gtccaccctc
    24661 aagccttctt gggtacaaat gtttcagtgg gtccctttac agtaatagaa gagggggtaa
    24721 ctgtaggaga taatagtatt attttttctc aggtttacct gggaaaaaac acccaggtag
    24781 gcagagagtg tgttttttac cctcaagtag tggtaagaga agattgctgg attggagacc
    24841 gggtgattct tcatagtgga gtggtaatag gggcggatgg ttttggctat gagaaagtag
    24901 ggaatagtta ttataaaatc cctcaggtgg gaaaagtggt aattgaagaa gatgtagaaa
    24961 taggtgctaa ttctactgtg gatagagctg ctctgggggt aaccagaatc ggcgctggtt
    25021 ctaaaattga cgatctggtt atgattgctc ataacgtgaa aataggtaaa aatgtggtga
    25081 tagtggccca gtcaggggtg gcaggaagca cagaagtagg agacgaggta gtaatgggag
    25141 cccaaagtgg cataatagac caccgtcgag tgggtaaagg agtgcaaatg gctgctcgag
    25201 cggtggtaac aactaatatt cctgatggtg tcattgtttc tggttttcct gcccaagaac
    25261 ataagaaaga gttaagagaa aaggctctaa ttaggaaact tcctgaactc tgggaaaaag
    25321 taagaaattt agagaagatg gtaagtaaac aggttgaaaa atgagttcag tggttttgca
    25381 aagaactctg gcttcttctt gcactgtgga aggggtaggc ttacatagtg gaaaaccggt
    25441 aagggtgact cttttccctg cttctgaaaa tagtggtatt ttctttcgag ccggggaaac
    25501 aattattcct gctgctatac aatatgtaga agttactcct cgttgcacga cacttaagaa
    25561 ggggaaaaaa caaatatcta cagtggagca ctttctggct gcttgctggg gtagcgagat
    25621 agataattta gaggcagtag tggaaggtga ggaacttccg ggaggagacg gaagttctat
    25681 tttctggttt aatgtttttc ggaaagttgg ctttcaaatt caatcatctc ctcggaagta
    25741 tttaattgtg aaggaaaata ttaaagtagg ggaaaatcgt cattatcttt ttgcttttcc
    25801 tgatgaggag tttcgagtat ggtattttct agatggggga gagaatggtc atttttctca
    25861 agctctagaa ttttctgaga atgatgactc tgataagctt ctttctgctc gtacttttgc
    25921 tttttcccgg gaggtggcag agattttgca aaaaggatgg ggaaagggga taagggaaaa
    25981 agcttttatt ctgaatgaag aaggggaaag ctctattcct ctgcgctttc ccggggaagc
    26041 ggcgggacat aagattttag atttaatggg agatttaatg ctcacaggaa agagagttaa
    26101 gggatgtttc ataggtattc gctctggtca tgctttaaat cgagaaatgg taaaaagaat
    26161 aacggaggca agtagtgaag aaaattgatc ctacggctaa gattgagaaa gaagttgaga
    26221 taggagaaga ggtagaaatt ggtccttatt ctattttaca aggggaaata aaagttggtg
    26281 ctcacactaa aattggtgct tttaccgtta tggagggtaa ggtaaaggtt ggtccttatt
    26341 gcaatattgg tcatcatgta gtgattggta ctcctccgca agacctatct taccgaggag
    26401 aagaaactca ggtggtaatt ggggacagga ccactctgcg agaatttgtt actgttcata
    26461 gagctactgg agaggggaaa ataacttcta ttggtgatga ttgtttaata atggcttact
    26521 gtcacattgc tcataattgc cgtttaggaa atggaattac tatggctaat agttctgcct
    26581 tggctggtta tgtggaagtc gaagatttag ccaccttgag tggctgggta ggagttcatc
    26641 agtttgttcg tttaggtaag ttagttatga taggaggtct ttccaaagta gttatggata
    26701 ttcctcctta tactttagct gatggtcacc cggctcgtat tttcggtttg aacgtggtgg
    26761 ggatgaaaag aaggggaata gaaaaagata aaagagagga aattcaccag atttatcgct
    26821 tgatttatca taataattta cctcgctctc aagctcttca ggaattaaaa aggagtagtt
    26881 ttgaccctca attggtagag gaaatcgttg gtttttggga aaagagtaaa agaggggttg
    26941 ctcggtgggt aaaagggagg aaagatagtg atgaagagga tacttctcct agctggtgag
    27001 ggtgatattc ctcttttgat ttatgaaaaa gccaaaaaag aaggagaaga agttttagtt
    27061 ttaagttctc tttctttttc tcttcctgaa gagttgaaac ccgatctgtg gatagatggt
    27121 atatctttaa ctcagatatt gaaagttatt cgggaggaaa aaataaccca tctatgtttg
    27181 gcaggtaaaa ttcctaaaaa tttgattttt gaagaagata ggtttcaaga agaatttaaa
    27241 aagttgtttt ccgctcctaa attacgggat ggagttctct tacaagctgc tttagaaaaa
    27301 ttgaaagaag aagtagatat aatttctcct cttttcttta tgaaagagta tctcactccc
    27361 tcaggtaaga ttgcaggcag agacccatca caagaggaat gggaagatat ttcctatgga
    27421 gctcgaattg cccgtttttt ggctgatgaa gaaattgggc agactgtggt agtaaaacgg
    27481 ggggcagtgt tggctttaga gggagcagaa ggtacggatg agactattaa acggggagtt
    27541 tcgctgggga aagaagtggt agtagttaaa atggctcgca gccagcaaag ttttctgatt
    27601 gatatcccgg cgatagggga gaagactgtg cagtgtctta aaaatggagg aataattgct
    27661 ctggaggcag ggaaaacttt ccttctggac cgggaaaaaa taggagaaga agccgaaaaa
    27721 tggggagtgg gagtaatagg aattaattga atgagtaaac tttttatttc tattggtgat
    27781 gcctctggtg attactatgg aggggaaata atttaccttc tccggcagag aaacaaagaa
    27841 atagaatttt tagctctggg aggagaaaga agccagaagg ctggtgctcg acttctttac
    27901 cccacagttt ccctttccac tgtgggtttg tgggaggcat tatttactct ccgggaatgg
    27961 aaaaaagttt ggactttgtc tcaagaggtg atagagaagg aacatcctga tgtagttttg
    28021 cttcttgata atcctgggtt taatcttcgt ctggcttctt tatgtcagga gcataaaatt
    28081 cctgtgattt attttgttcc tcctcaagta tgggcttggg gaagaaagag agcctccttt
    28141 ctttctcagc atgccgattg gatattgact attttcccct gggaagtaga ttatttccag
    28201 ggaggaaaag cgaaagtaaa gtgggtgggg catcctgcca ctcaccttat ccctcctgcc
    28261 cggttttctc ctctggagga gaaaaagaag atagcccttt tacctggtag ccgaaaaaga
    28321 gaaattgtta attatttagc agtgattaaa gaatttattc cccttctcct aggtaaagga
    28381 atagtggaat ttactgcaat tgctgcctca aaagaagcgc aagaaatttt agtaaaagaa
    28441 atggctggtt ttccagtgac tataaaaaac aggagagagt tgctctctgt tttatcgggt
    28501 agttctttag ctatatcttc ttctgggact gtaactttag aagtagctct ggctggaatt
    28561 ccccagatta tagtttaccg ggtggctctc ttaacttttc ttgtagctcg gttggtaatg
    28621 ggaaaaaccc ttataggttt acccaatatt gtgatgggaa aagaaatatc tccagaatta
    28681 atagggaaag acttttctac ctcccgtctt ctaaaggaag tagaaaaaat attaaataat
    28741 cctcaagctc gggaaaaggc actggagaac tccttaaaga ttagagaaaa gttaaatcga
    28801 ggaaaccctt ttgaagaagt ggtccaggtg gtgaaagaat atctctgacc gtcctttgtt
    28861 acttataacc gtgaatagtc cgggggaggt gtattattgg ttgtatcccc ttttatgtga
    28921 cctttctcgg cagccttctc ctcctcgagt atgggtttat gttcctccct gccaatttag
    28981 tactggtcga gaagaagaag tagtcaaaaa ttaccccttg gtggataaag tattttctcc
    29041 tcgagagaca gtatcttttt gtttgagaaa aggaaaattt tcttctcctg gtttgatcct
    29101 ttatttagga ggagatttgt tttatgctgt tttactgaaa cgaagaacag gttttcccct
    29161 ctgggtatat tgtggctatt ctaagtggtt gaataagata gacttatact tagctcgatt
    29221 ttggggagat tttgagaaac ttgattttga tagaaaaata tttttggggg atttactttt
    29281 ttcctctata tctactctcc cttcttctgt tttccctcct ggttctcctc gtttcctttt
    29341 tttgcctggt agtaaaaaag ttttttatcc ctatttgttg ccttttttct ttcaagtggg
    29401 gaaagaactt aagagagttt atcccgaagt ctctttggct ctaagttttc ctgaccacta
    29461 taattttaat gatataccag aattaaaaac catacctcct ttctttagtc ccttttttgg
    29521 taaaacctcc tctcttatgg ccgaggctga cttaatagtt accatacccg gttctaataa
    29581 cttagaaatt gcttaccggg ggaaaagagg tatagttatt cttccttttt ggggattaga
    29641 aaaaataccc ttagaaggtc cgatgttgct cttagaaaaa ataccttttt ggggtaaaat
    29701 aataaagagg aatgtggtaa aaaaaatggc tgggacgaga aagtggattt ctctccctaa
    29761 tgagcttttg gataaagaag tggtgaaaga gctgagggga aatctatctg tatcggaagt
    29821 actggaggca attcaggaga tgttgaataa agagccagaa atttacttcc ctgaagagcg
    29881 ctttccccgc aatgcgtctc cgcgtctagc ggagatgatt ggagaatggt gggatggaaa
    29941 agcaaaatag tgcttttaac cgtatttgga ggtatcttcg cccttataag aattacgtta
    30001 taggtgcttt tttgatggct ctttttggag ccggatttaa tttagccatt ccctggttag
    30061 tcaaggggac catcgataat gctctggtag gcaaagataa tcgtcttctc ctccttattt
    30121 cccttggagt ggtggttctc tttttcttta aaggattatt ttcctatctt cagaatttat
    30181 ggatttctta ttctggtttt cgagctataa cttgcctgcg ttctgaactc tatcagcatc
    30241 tttattttct tcctgcatct tttttccggg acaatcccgc aggagaagtg gttagtcgct
    30301 tgactaatga tgtggcaatg ttgcagaatc tcttttctaa cgctttcgtc aacctattta
    30361 tggacttctt tattttttcc ggctccataa ttttgttatt ctttattcac tggaagctgg
    30421 ctctattttc agtggcggtt ctaccactgg tggggttcag tgtagattac ttgggtaaga
    30481 gaatcaaagg agtttctcat ctcctgcaaa aaagagtggc ggcattgact tctttaatag
    30541 aaagagcagt agcaggtatg aaggtttttc aatcctatat gacaggtaat tatgaaactg
    30601 aaaaatttga gatggaaaac gaggcaaatt ttgctctggc tatgaaacaa gcccaggcta
    30661 aagcactgct tactcctctt gtggaattag tttctgcctg tggtgttatt gctgttttat
    30721 tatatggtgg aagagaagtt attcagggtc atctcactgc tggtgggctg atcgcttttt
    30781 tggggtatct ggttactgct gcgtctcctc tttctcgttt ttccagcagt tttcaaattt
    30841 ttcaacagag tttagcttct gctgagcggc tttttgattt tttagaccta cctctatggg
    30901 gcagggagga agaaggagaa aaagaaataa cctctttgca ggaaggaata aggtttaaaa
    30961 atgtttcttt ttcttaccag gaagaaaaag tattgaagaa ctttaatctg gaaatcaaaa
    31021 agggagaaaa agtgggaata gtagggcata gcggggcagg gaagactacc ttaattaact
    31081 tgcttctccg gttttatgac ccggaggaag ggagtataga aatagacggt attgacattc
    31141 gagagttaac cttttcttcg ttaagaaatt tgatgggggt agtgttacag gataccctgg
    31201 ttttaggggg cacgataaag gaaaacattc tctatggaaa cttagatgct actctggaag
    31261 aggtgcgtct tgcttctgtg agggctcatg ctcatgaatt tattgttcat ctggaaaaag
    31321 gttatgacac tgaagtggga gaagggggag ctcgtttctc aggagggcaa aaacagagaa
    31381 ttgccattgc ccgggcttta ctaaaagacc cccctatttt gatttttgat gaagcaactt
    31441 ctaacttaga tccggaatcg gaaagatata ttctggatac catcgctaat gttgggaagg
    31501 ataaaatagt gatagtagta gcccattctc taagtatggt gaaagattta gaccggatta
    31561 tcctcgttga agacggagag gttaaaggtg atggaacgca tgaagagctt cttcagtcta
    31621 gcttctatta tcggcgtctt tttggcatag agaattttga taaagttctc tgattttttg
    31681 aagggagggg cggtactcag gactctggta gtcgggatag gtataaggaa aaggccgaaa
    31741 atctcccttt tcccatttaa tagtagcttc agcatatatg ccttgccgga gataaacacg
    31801 gtgagagaaa tttttagtac tggctagcac gattttccct ccgtcgaagt aaccggggtc
    31861 taaattgatt ctccgttctt tttctccttc tttagtagct ttttctattt cgttagtgat
    31921 gacttttata tcagctaact cttcagggtc gattaaggat ttaaaaacta taaatactct
    31981 tttcaagttt tctccaatgt tccgataata actggtataa ttgaaaggaa tttcctcgct
    32041 aatccactca atttctcccc agtatttttc tatctggggc aaaatatttt ctaatatatt
    32101 tctttcttca taaagaacag caataaatag ttttacgggc tgagggtttt tgatttctcc
    32161 cattttcttt taccttctta tattttatca taagagaaca gatgtgatga gctgtacata
    32221 aaagatatag ttcgaaccag gaaaaaaatt ttgaccttac ttgacaaaag ctttaattgg
    32281 tggtttaatt tgatggtcga agaaggtaaa attttaacct tgtggaagaa aggggggcag
    32341 caatcttgac ggaagaaaaa gaaatgctta aagagcccga agagattaat aaaaatagag
    32401 agatggaaga aagtggtctc tctgaaatag aagaattgca ttctctaata gaagagcaaa
    32461 agaaaataat agaagaaaaa gaaaagataa ttaaagatta tattaatgat ttgaaaaggt
    32521 tgaaggctga ttttgataat tttcgcaaaa gagagactct ctttcgacag gattttgtaa
    32581 aaaaagctaa tcgaggcctt atatttgagc ttttgcccat cctggatgat atggagagag
    32641 ctatacggga ggcaaaagct agagaggtag atgaagctat agttcaggga atagaattaa
    32701 tttattgtaa gtttttaaat attttggaaa aagagggcgt taagcccatc gaaaccgaag
    32761 ggcaaaagtt tgaccctaaa tatcatgaag caattgctac ggtatctctt cctgattatg
    32821 atgattatga aatagcagag gagatacgcc gtggttatct ttatcaggac gaagttttac
    32881 gtcctgctca agtaagagta aacagaattt ctaaagattt tatggcctga ggaggatgat
    32941 agaaaatggc taaagtaatt ggtatagatt tgggtactac taactcggta atggcatact
    33001 tagaaggagg acaaccagta attattccta ataaagaagg gtctcgtctt actccttcgg
    33061 tggtagcttt taccaaaaat ggagaaatac tgatagggca gttagctaaa agacaggcta
    33121 taactaaccc taaaaatact atatattcca ttaagcggtt gatgggacgc cgctatgatg
    33181 atccagaggt agaaaaggcg cgtaaagttc taccttatga aattgttcct ggcgaacatg
    33241 gtgaagtgtg tgtaaaagtg ggggaccgag tttatacttc tccagagatt tctgctatga
    33301 ttttgcggaa gatgaaagaa gatgcagaag aatatttagg agaaaaaatc actgaagcag
    33361 tgataactgt acctgcctac ttcaatgata gtcagagaca ggccaccaaa gatgcaggaa
    33421 aaatcgccgg tctggatgta aaaaggatta ttaacgaacc tactgctgct gctctggctt
    33481 atggtttagg taagggcaag gaagaagtaa ttgctgttta tgacttaggt ggaggaactt
    33541 ttgatatctc tattctggaa atcggagaag gagtgtttga agtaaagtct acctctggag
    33601 acactttctt aggcggagat gacttcgatc gaagagtaat ggactacatt atcgatgagt
    33661 ttagaaagga tcagggtatt gatttgcgag gagaccagat ggctcttcag agattaaaag
    33721 aagcagcaga aaaagctaaa tgtgagcttt ccactgcttt gcagacggaa ataaacttac
    33781 ccttcatcac tgctgatgcc agtggtccta agcacctggt aatgactcta actcgcgcta
    33841 aattagaaca attgactatg gacctgattg agaaaactat aggaccctgc gagcgagcct
    33901 taaaagatgc tgggttaaag cctgaggata ttgatgctgt ggttttagtg ggtggtatga
    33961 ctcgtatgcc taaggtgcaa gaagtggtgg agaggatatt caaaaaagaa cctcggagag
    34021 gagttaaccc tgatgaagta gtagcagtag gagcagcaat acaagctgga gtgctgaaag
    34081 gcgaagtcaa agacgtgctt ctcttagatg tcactcctct ttctttaggt attgagactt
    34141 taggaggagt tttcaccaaa ataattgaac gtaatactac cattcctgtt tctaaaagcc
    34201 agatttttac cactgctgca gataaccaga ccacagtaga agttcatgtc ttacagggag
    34261 aacgacccat ggctcgggac aacgtttctc tgggccggtt ccagttaact ggtattcctc
    34321 ctgctccacg aggtgtacct cagattgaag taaaatttga tattgatgct gatggtattt
    34381 tgcatgtttc agctaaggat ctagctacca ataaagagca gaccattacc attaaagctt
    34441 cgagtggtct ttccaaagag gatattgacc ggatgattag ggaagcggaa cttcatgctg
    34501 aagaagaccg ccgcagacaa gaagaagttg aagttcgcaa tcaggctgac aacctggttt
    34561 atactgtgga gaaaaatctg agagaatcag gagataagtt atcttctgga gtaagatctc
    34621 aggtagagag tgctctcaac gctttgaagg aagctatgaa taaagatgat atagctgata
    34681 tcagaagaaa gatggatgat ttgcagaatg cttcccacgc tatggcacag gagctctatc
    34741 aaaaagctgc tcaatctgaa acttctactt ctgaccaaac tggtagttca ggtggagcta
    34801 ctcaggatga aaatgtagtt gatgccgatt accgggtagt agacgatgac actaaataat
    34861 gaagggggta tgtccccctt cattatttag tggaaaattg tgcttaattg tagaagagga
    34921 aatttttaaa ggttagcttt ccgtaaaaga acatattttt tattgtgcac tgctaaaaga
    34981 tgattggtct ttggaaggac cgattaaacc gcttcgataa gcatttagaa aatttctaca
    35041 gtatttatct ttgcctgcta tagcctccgc ggctaggagt gccgttctcg tttctttgtt
    35101 acagggatcc ataattgcag aatccattcc agcaaaaatg gcaactgtga gaaaggcatg
    35161 attaattatt tttctcaagg gcattccgaa agaaatgtta ctgagacctg aagtggtttt
    35221 gatatctgga tataattgtt tgatggcctt aagtgtttca ataaactttc gtaccgattg
    35281 gttgtcagta gcgagcgcta ctaccagagg gtctatatag atttgctcag gtttaatgtc
    35341 atatttctgg gctttttcca ccagcgattt ggcgatatct attctggttt gcacatcagc
    35401 agggattcct ctactatcgc aagttaaagc tataacctgc cactgacttt cctggataag
    35461 aggataaatt atttcacatt tgctcccctc tcctgataca gagttaatta ttcccggctg
    35521 gtgtgcgtag ttaaacactt gttcaattat ggcagggttg ggactgtcaa tacataaagg
    35581 cttatctatt gtatcctgaa ctatatccat cagccatttc aaaatttcaa attcaacatc
    35641 agatgtagtt cctgcacaca catcaatata atcagctcct gcctctacct gtctccttgc
    35701 taaatctctt ataaaatttt catttctctc ttctatagcc ttctgaacat caggaatagt
    35761 accgttaatt ttttctccaa taatgaccat tttttctctc cttttatctg aatgatatta
    35821 cgagtattaa taattatata ttgttttaca taaattatta atacggatgt ttaggctggt
    35881 ttttaagcct ttttctcttt gaaagcggat agtcctttta tcatgatttg cgagtcccga
    35941 gtgaggattg acaaatttct aggggtagaa taagctttat aaagataagt gtttttttat
    36001 gatttgcgta actaaggagg tgaaaaacca aattaaagaa ctcaaaattc tttttaaatc
    36061 attagccata cattaactgg aaaggagaag gtaaagtgca agaagagctt ttcaacaaaa
    36121 tagtaaatat ggatgaggaa gggtctatta aattagctaa agagtattta gataagggtg
    36181 gcaaacctca agagttactg gaagtttgca ggagggcaat ggacgcaata ggagagaagt
    36241 ttgaaagggg agaatatttt ctttccgaac ttttactggg aggagaaata tttaaaagca
    36301 tcatggagtt tgttctgcct ttgattgaag aaagtgaaac tacgagatta ggtactatta
    36361 tcctcggtac tgtaaaggat gacatccaca acataggtaa agacatattt aaagcttttg
    36421 ctcaagcagc agggtttgaa gtaatagatc tgggagttga cgtaccggaa gaaaaatttg
    36481 tagaagcagt gaaagagcac aaacctgaga tagtgggcat gtcctgctta ataactgccg
    36541 gtataagttc aatgaaaagg acaagagagg cattaagagc aagtggttta gagaacgtga
    36601 aaataataat aggcggagga agagtagacg agagtgtgaa gcaatttgtg ggtgcagatg
    36661 cctggtctga tgatgcagca agaggggtga ggatttgtaa ggaattaata ggagtggagg
    36721 ggtgataaga gatggcaaaa agtccagagg aacttgcaaa agagcggctt gctcgtatag
    36781 aagcggctat taatttaaaa gagccagata aagtaccctt atggggggtg gggggagata
    36841 tagtagctgc ttatgcaggg atgactcagc atgagttttg ttatgattat gataaagcag
    36901 taaaagccat tgaaaaattt atgagggatt ttccttttga cacttctatg gccgggctga
    36961 tgggcttaga aggacgaata ttttgtgtag ctttttcgga attccccgat atttctccgg
    37021 ctctctcgct tattaccgga ccaatgcatg acgtactagg tgataaatac tacagatatc
    37081 cgggtagaga gattgatgaa aatgccactc ctcagtttat tggtggcact ttcatggagc
    37141 caggtgaata tgataggtta attgaagacc cagtagggtt tgttgctgag actatacttc
    37201 cccgagcatg tacaaattta gatactcccc ggaaagctat ggctacctgg gtgaggttgg
    37261 ggatggaatg tagcaagttt ttttctgcag ctgctgaaat tggcagagtg tcggcagagc
    37321 tgggatatcc accttttcca atgggatggg gatattcccc tctggacatc attggagatt
    37381 tcttgagagg agtcggtact gtagtattag atatttatag atatccggat aaagttaaaa
    37441 aagctactga ggcactaact gagccgattg caaattatgc gctatatcat aagtatatgg
    37501 gagccaaata tgcaatgatt ccgctccacc ttaatgaata tctatcgcct aaattgtata
    37561 acgagtttta ctggccttac ttaaaagcgg ttattctcaa gctttatgag gaaggtatca
    37621 agtcaatggt gttttttgaa ggccatcacg atgcccattt ggataccata ctcgaactac
    37681 ctgaaggatg gggtatagca tattttgaaa agaccgatgt agttaaggca aaaaaggttt
    37741 tgcaaggtca tacttgcgtt atggggggta ttcctatggg tttactggtg ggcggtacac
    37801 caatgaagat agaagagtac atcaaaaact tattagaaga ggtaaaacca gggggaggat
    37861 ttatactcgc cccgggagtg gggacagctc ctagtgaaac accaattgaa aacatacatg
    37921 ctcttattga agcagtagaa aagtacgggt attactagag aattaaggta agggcatcga
    37981 tgtagtataa aatttatact tctccgaaca ggtataaact tagatactat ctgggaagct
    38041 ataactaccc aggtggaaat agaggggaag caaggaaagt gcagcctgta gcggtagaat
    38101 ggccaatgtc ttcttaatat tcgtaagaga cattcagaag aaattaagaa gatgacagct
    38161 ttgaagttga attagcttgc gagttttatt gaccttgctt agagaagcaa ttatttccaa
    38221 accttatgaa gagagcatta aaccagatga agcctctgaa gacattgata tctacctata
    38281 atctacttga ggaggtgaag tacgaaggag gttatatttt aatcccgaag tattgcgtca
    38341 caagagacac cagttaaaaa tataactttc ctctccatga gtagcggaaa agaagagtta
    38401 acataggtct tgtgtcgaac gctcataaga aggggaatgt aatttttgat tgattatcac
    38461 ttttaagtaa ttgtgcattt taaaaggaga agaaaagggt ggaaagtaaa aagaccgttg
    38521 caattgctat tatgtcggta ttctttatag cgatgggtgt aggcacaatc actcctgcga
    38581 ttcaggatat agctgaagcc tttccgcatc tcagctttag taccattttg cttgtgttta
    38641 cactgccctc tctgtttata attccatctt cgattttagc aggagtagca gcaggaagca
    38701 agataaaata tcgaactctc ttaatatggg gtacattact tttttcactc gccggtgtag
    38761 cacctatttt catgagcaat tttactacta ttttagtagc cagagcttta tttggaattg
    38821 gtcttggaat aattgcggct ttgggaaatg ccctcgtcct ggctttctat gaagggcaag
    38881 aaagagcaaa tatgctggga ctgggaggag tgatgatgaa tataggtggc attgtgctgc
    38941 aactactggg aggatttttt tcttctatca gatggaattt agcgtttttg ccccatctta
    39001 ttgggttgat cacactcata atggttactt ttatgctccc cgaaccagag aaaagagaag
    39061 agataattgc aaaaaaggta actctaccct ggagtgtata tttaatagcc ataatattcg
    39121 gggtaattac catgctcaac tatccaactg gcgcaaacat gtccactata ataattactg
    39181 gtaatttagg agatgctgct tcagcgggga ttgttctttc actatttaca gtgggaggga
    39241 tggtggcagg agctgtattt ggaaaactgt atcaaaaaat tgctcttttc tccatcccgg
    39301 ttggatttgc agtaatggca gcaggagcag gacttgtata ttatgccaat agtctatcta
    39361 tattagcact gggtatggct tttataggga tgggttttgg ctggctaatg cctgctttta
    39421 tgatgatggt aggtatagtc acttctccgg aagcgtatgc tactgcctca gggatactga
    39481 tggcgtttat gaatttagga ggatttttgt ctacttacta tattgctatg ctcttgaggg
    39541 taaacagtgc gatacgcttt cccatctttg tagaaatggt tgctcttttc attggtactc
    39601 taatatatct aattttgagt ggaagcattc atcgttctct tcttcgggac gagcctacag
    39661 cttaagtatt gcttatcaat tctcctctct gtagtgttac aggaatttgt atacttgtga
    39721 tgctacagag agaaaggttt ttcattattc cagtgtttct tggggagaat ctttttgtct
    39781 caatttcagg agctgagata atacttttct ctctcctcag gtaatttttt gtgttgtttc
    39841 cctgctattc gttgcgatat agaatattaa aggcatagca ttttgtcttt cttagatttg
    39901 tgttactatt taactggtaa attttattaa agaagtaaat aatctgcttc aggcggtgcg
    39961 ctcataaatg aatagatata aagtaaccat acttccagat gatgttcttc tcctagtaac
    40021 agagggcact tttttgaaag aagtttttat aagaagtgga atagattttg aatttccttg
    40081 tggggggatg gggttatgta aacgatgtaa agtaaaaatc attaaagggg atggcaaaaa
    40141 agaagatgct tttgcctgtc aattgaaggt agaaagcgat ctgatggttg aaatatcaag
    40201 tagagaagag aggcatacaa tattaacaga gggtgtagag cgacgggtgg tagtagaccc
    40261 tttagtaagg aaagtttatc tggaactgcc tctcccttct ttaaaagata atcgcgatga
    40321 ctgggctaga ttggtaggaa aagaaggaat aaagccggat cttcctgtaa tacaggattt
    40381 gccggaaaag ctaaggaaga gcaattttaa ggtaactgcg gtagtgggag accataagat
    40441 tatcagtgtg gagggtggag atacaagtaa caaactatta ggtatggctt ttgatatagg
    40501 gactacaacc gttgtaggct accttataaa tttgaaaacc ggagaggaaa taaccaaagt
    40561 ttcatcactg aactctcaaa caaagttcgg tgcagatgtg atttcacgta ttacctttgc
    40621 ttctgatacc cctttggggt tagataaatt gcatagagaa attataaata aattgaacaa
    40681 cttgatagga gaagcggtaa acacaactgg ctatgaagct gaaaatatat acactattgt
    40741 ggtggtggga aataccacca tgcaccatct ttttcttaag atacagcctg gatatttagc
    40801 tcgttctcca tatgtaccgg ctgtgacaga accaatggtt gtggatgcaa gtgatatcaa
    40861 tctcagaata aatttagcag ggaaagtctt tacctttccc aatgtagctg gctttgtagg
    40921 aggagatctg gtagcagatg ctctggcttc agagatggat aaagctaaaa aactaaaact
    40981 tttagtagat ataggaacca atggagaaat aatgctcggt aaagagggga aatacctggc
    41041 ttgttcggca gctgcgggtc ctgcctttga aggggcacaa atatcatgcg gaatgagggg
    41101 agcttcaggc gcaattgctt atgtatccat ggatgaagga gattgtaaat atacagtaat
    41161 tggagaaacg ttgcctagag ggataacagg ctctgggctt gtggacataa tagctgagct
    41221 tctaaaaata ggaatggtga ataaaaaagg ctcaatgctt gagtctgata acataaaaag
    41281 cgagctgggc aaagaatata aggacaggat tatcagtatt gacggtattt tagcatttgt
    41341 agtggctgaa aatacagcta caggacaacc ggtatacata aatcagaaag atattagaga
    41401 atttcagctg gcaaaaggtg caattgcagc ggggattgaa atactcttaa aaacttatgg
    41461 ggctaaagtt gaggatatag atgaagtatt tcttgctggt gcttttggaa actacctgag
    41521 acctgaaagc gcctgcagag taggattgat tcctcaagag cttgagggaa gaataaaagg
    41581 aattggaaac gcagcaggta tcggtgcaaa gctggctatg ctttctaaag aagagtatga
    41641 acgagcgata gtactctctc gaaaaacaca ttatatcgag ctcagctccc accctgaatt
    41701 tgagcgtaca tttttaaata agctggattt ctaacaatta aaagtagaag agtttacttc
    41761 cagggatcgt caaaatactg aatgaagagt tatatgttga aataaaattt atctcgagag
    41821 aggtactctc atgtgttcag ctatgcttta agagagtgaa aagatgaaaa atattgttat
    41881 tgcctgtcaa acgataaaag atgagttgac tctggctatc aaggaaacac agtgcgaata
    41941 tccggttatc tggatagatt caagctatca tttatatccg ggcaaattga gattaaaact
    42001 tcaggaagaa ataaacaatt tgaaagactt tgataatata ttgctggcgt atggtttttg
    42061 cggtaatgcg atagcgggtc ttaaagcttc tagcgctaat cttgtctttc ccaaaactga
    42121 tgattgtatt tctatagttt taagtaaaga cggggaactt cggcagatga aaactgctta
    42181 ttttttaacc cggggctgga tagaaagccc tggaggcata atggtagatt attcaaatgc
    42241 tttaaaacgc tatggagaag aaaaagctaa acggatgttt agtcttatgc tagagcacta
    42301 cgataagtta gtagttatag atacaggtgc ttataacata gtagactata tcgagaaaat
    42361 acagaaattc gctaaaatca gtggtttgga tctggtggta gagaaaggtg gtatagggct
    42421 tttaaaaaaa ctgctgaccg gaccttatga cgataaattt tgcatcatag agaaagggca
    42481 agaggtaaac atcgatgata tattattgtc ttaggtgaaa ttggaaagag cagtttcctg
    42541 tagcataagt tttaagatga taacgctgga aaatgcatcc cagcgtggca tcttgggttt
    42601 tataggaggc atttcttcct tatagttata tggcagattt ttcctctacg aagggtagca
    42661 atgataacaa gttttgcggg atgctataga tattggtggt tgattaccgg agtgaaaaat
    42721 taaaaggtgt ttcaatattt agcaaaaatc atgcaaggag attgccagat ggaagaccac
    42781 agctacttcg tcgggcatat taacagcagt aaaattggat tt
//
LOCUS       DAOSVE010000005        36963 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_783_length_36963_cov_69.526444, whole genome shotgun sequence.
ACCESSION   DAOSVE010000005 DAOSVE010000000
VERSION     DAOSVE010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 36963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36963
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_783_length_36963_cov_69.526444"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="PK016_00880"
     CDS             <1..259
                     /locus_tag="PK016_00880"
                     /inference="COORDINATES: protein motif:HMM:NF013753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXL01666.1"
                     /translation="PQKADSLLAQYPTLQGFYWAKEKIRELYRQKDREEAAKLLDNII
                     FNLKIADDAELVRWGNTLKKWREPILNYFHNGTTNGYTKIL"
     gene            583..1215
                     /locus_tag="PK016_00885"
     CDS             583..1215
                     /locus_tag="PK016_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HXL01667.1"
                     /translation="MNPINFEQNKRLEKQINFIIEIDKVKNIIRKSLLFDGSRFENVA
                     EHSWTISIMAVLLEEHANFKVDTGKVQFMLLIHDIVEADAGDTFLYSEDRKNAHINEE
                     KAAERIFGMLEPDQKKIFLDTWKEYEEGKTNEAKFALVFDRLQPIIQNYVNKGFTWKK
                     HNVTYEQVVKENKHIKEGSEEIWSFVEHILQESVEKGYLTKNQSLEEEKK"
     gene            complement(1354..2214)
                     /locus_tag="PK016_00890"
     CDS             complement(1354..2214)
                     /locus_tag="PK016_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HXL01668.1"
                     /translation="MDKLTIYQRPLLSNPSLLIGFTGWMDGGDVSSGSVAYLQKKFAA
                     EMIAEIDPQDFYLFNFPGSMEEVAQFRPYTSIKEGIVTEFHYPQNQLFCASKENLLLF
                     LGKEPNMKWNEYAENIFALGKEFGVKRIYFIGSVSGLIPHTREPRVSCSVSDEKLKEE
                     LKDYNFRFNDYEGPASITTLLTLMASHRGIEMINLVVEIPMYIQATNPRGISSVLRIL
                     SSLLNLPLVGEDLENMREEFDRSVEKLVKNYPDLVERIKKLEENYDREILEDDQSFRD
                     WLKRHGIDKI"
     gene            complement(2366..3685)
                     /gene="dnaB"
                     /locus_tag="PK016_00895"
     CDS             complement(2366..3685)
                     /gene="dnaB"
                     /locus_tag="PK016_00895"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HXL01669.1"
                     /translation="MSIERVPPHDLEAEQATLGSMLLDKEALLRVLDILQPEDFYKEN
                     HRLIFESIVELFEEDQACDIVTLVAKLRQKNQLEAVGGMDYVTQLVNIVPTAANAEYY
                     AHIVEEKSIFRSVINLCTRIIRESYNSDLDAKELLDRAQNWILQLSQKKIRYDFVPLK
                     AIINEAFDRIEELYHRDEHVTGVPSGFVDLDLITAGFQPSDFVVVAARPGMGKTSLCL
                     NIAQYVGTKKNVPVAIFSLEMSKDQIAQRMLCSEARIDAAKVRKGFIAEREWPELARA
                     AGRLAEANIFIDDTPSISVLELRAKSRRLKAEKDLGLVIVDYLQLMRGYGRPENRQQE
                     ISEISRSLKAMARELNVPVIAVSQLSRAVEQRTPKRPQLSDLRESGAIEQDADLVLLI
                     YREDYYNPNSSKKGIAEIIVAKQRNGPIGTVDLMFQKDYARFENLAR"
     gene            complement(3682..4155)
                     /gene="rplI"
                     /locus_tag="PK016_00900"
     CDS             complement(3682..4155)
                     /gene="rplI"
                     /locus_tag="PK016_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HXL01670.1"
                     /translation="MKVILLQEVENLGREGDVVEVKSGYARNYLLPRKLAVEANKGNI
                     TQIEALKKKRAIREAKELEEAKALQGKIEGLILEFKKKAGKTGKLYGSVTSREIAEEI
                     SRIAGINLDRKYLELEESLKEIGEYPVKVNLGKGVYATVQVKIQGEEVPEEAEES"
     gene            complement(4168..5076)
                     /locus_tag="PK016_00905"
     CDS             complement(4168..5076)
                     /locus_tag="PK016_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HXL01671.1"
                     /translation="MRQARSTAEGAFLAALTVVFYLSSIYIPILGFLLSFLCPLPVMF
                     LTIRWDMRAGLLAALVASFIVFIFAGVVSALTCFVGFTLLGLIMGLTIKRGYNFIEVI
                     GINTLVSISSKLLLIGLVILIMGQNPLAESLSFLEEGLKKGLSFLPSEGGINVEDIIN
                     LIRMALPATVIVASILDTVLNFLLGGWIGKRLGIKFPPFPPFGEWQLPKSIFWAFALG
                     WLFVLFGGTTFWGKIGLNLQMVTQILFLVQGASVVYYFLGKYIRSRVWLTIIVIFLAL
                     QPLLSTILSWLGVFDVWFDLRKIRGK"
     gene            complement(5082..5333)
                     /gene="rpsR"
                     /locus_tag="PK016_00910"
     CDS             complement(5082..5333)
                     /gene="rpsR"
                     /locus_tag="PK016_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HXL01672.1"
                     /translation="MAVDKDEYSGRKKFFRRKRKPCMFCLGKATIDYKDADFLQRFLS
                     ERGKIVPRRVTGTCAKHQRKVAQAIKRSRELALLPYTID"
     gene            complement(5320..5694)
                     /locus_tag="PK016_00915"
     CDS             complement(5320..5694)
                     /locus_tag="PK016_00915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HXL01673.1"
                     /translation="MSHLSLNRVFLIGFLPHSPVVRYVPSGIPVVNFSLAVYRTIPSE
                     EEKEWIDYLNIIAWRDLALFCGENLKRGDWIFVEGRVQARTYENSEGKKRRAYEIVAS
                     GVKLLSYPENKKREEVTENGSR"
     assembly_gap    5774..5873
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5874..6161)
                     /gene="rpsF"
                     /locus_tag="PK016_00920"
     CDS             complement(<5874..6161)
                     /gene="rpsF"
                     /locus_tag="PK016_00920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HXL01674.1"
                     /translation="MNMNSYELGVILRPTLSEEELSSEVEKLKNFLGERGEVVKVDLW
                     GRRKLAYPIQKYQEGYYAFFNFKLPPSQVAEVKRWISLDEKVIRHLLVKEDE"
     gene            complement(6219..7475)
                     /gene="ahcY"
                     /locus_tag="PK016_00925"
     CDS             complement(6219..7475)
                     /gene="ahcY"
                     /locus_tag="PK016_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HXL01675.1"
                     /translation="MEFEVADLDLANEGKKKIEWTEREMSVVTGIASSWRDTLPLKGL
                     RVAACLHVTAETAVLVRALQAGGAEIRLCASNPLSTQDEVAASLVKDYGISVFAIRGE
                     NHDTYYQHIQKVLEFRPHITMDDGADLVSFVHGHKRELLSEIKGGTEETTTGVIRLRS
                     LEKNGELGYPVIAVNDALTKHLFDNRYGTGQSTIEAILRATHILLAGKKVVVAGYGWC
                     GRGIAARAKGMGAQVAVVEVDPVRALEALMDGNNVLSMKKAAPWGEVFITATGNTSVI
                     RGEHFLSLPDGAILANAGHFNVEIDIKELEEMSVNKREVRPLVEEYTLKNGKRVYLLG
                     EGRLVNLTCAEGHPPTVMDMSFANQALSVKYLKENASYLEKRVYRVPEEIDREIAFLK
                     LQSLGLEIEELTSRQREYLSSWQEGT"
     gene            complement(7492..8685)
                     /gene="metK"
                     /locus_tag="PK016_00930"
     CDS             complement(7492..8685)
                     /gene="metK"
                     /locus_tag="PK016_00930"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HXL01676.1"
                     /translation="MEKRYQITSESVTEGHPDKISDQVSDAILDAILAQDPQGRVAVE
                     TLVATGLILIAGQVSTNSYVDIPHIARSTVRDIGYTRAKYGFDFETCAVLTAIDEQSP
                     DIALGVNRSLEVKMGEEVEDEELSLGAGDQGMMYGYACDETPELMPFPIILAHRLAFR
                     LAEVRKKEILPYLRPDGKTQVTVDYVDDKPVRIANIVVSAQHHPDISLDTLREDIVRE
                     VINPIIPPAYKDDNTQILVNPTGRFVIGGPQGDTGLTGRKIIVDTYGGISKHGGGCFS
                     GKDPTKVDRSGSYAARYVAKNVVAAGLASKCEFQLAYAIGVARPVSMSIDTKGTGKIP
                     DDEILRLVREVFDLRPGAIIKNLKLRRPIYKKTAAYGHFGRNDPDFSWEKTDKVDILR
                     EKAGL"
     gene            complement(8800..9174)
                     /gene="rsfS"
                     /locus_tag="PK016_00935"
     CDS             complement(8800..9174)
                     /gene="rsfS"
                     /locus_tag="PK016_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HXL01677.1"
                     /translation="MRIQEKIVLAKEAAESKKAFDLAVLDLRGIFPFSDYWIICSGSS
                     ALQTKAVAEEVLKKMEERQLRPSHLEGMEEGEWILIDYGDLMIHIFREGERLFYQLER
                     LWHRAPLLYSEKEGLNLLEKIN"
     gene            complement(9125..10066)
                     /locus_tag="PK016_00940"
     CDS             complement(9125..10066)
                     /locus_tag="PK016_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HXL01678.1"
                     /translation="MKNKRRSRKVFYILLGFFLGVFILFLSTLLGFRIPLRWTVVKEV
                     MGYSEPPNTFILVVGQDSIEPRRSDTIILVGINSQIDEVLLFSVPRDSRLLIPGRGYD
                     KVNHSYAQGGIGLLRQTLESALGIKIPYFVEVDYEGFEKVIDALGGIEIEVEKPLKYV
                     DKAQGLYIDIPAGKQLLNGEKALQYVRFRYDALGDIGRIRRQQKFLQALLDKVENDPL
                     LVTRIPQLIESSREALTTNLTGDYLTQLVMWFQGLSERIIRMETMPGEPTYINGVSYW
                     EPDLEASQEMVQKFFFARREDIENSGEDSISQGGGGE"
     gene            complement(10056..10619)
                     /gene="yqeK"
                     /locus_tag="PK016_00945"
     CDS             complement(10056..10619)
                     /gene="yqeK"
                     /locus_tag="PK016_00945"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HXL01679.1"
                     /translation="MDYIKIKDQIILTLKNMLTEARFYHCLGVAEEAQYLASRWGERE
                     DKLYVAGLLHDCARDLSSEELVPLLPPYLEEEAYSIPAIFHALAGPAIVEREFGVKDY
                     QIWHAIRWHATSNEAMSRFDKILFIADFCEAGREFPEANLIRGMAEKDWEEAYRQVLK
                     EKLSFLLSHNFLVYSAAWKAWNKENEK"
     gene            complement(10600..11169)
                     /gene="nadD"
                     /locus_tag="PK016_00950"
     CDS             complement(10600..11169)
                     /gene="nadD"
                     /locus_tag="PK016_00950"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HXL01680.1"
                     /translation="MGGTFDPIHHGHLVTAEEVRDYFDLDEVVFVPSARPPHKIGQEV
                     SDPEHRYLMAVLATVTNPYFQVSRVEIERPGPSYSIDTVRYFKNLWGEGTEIFFITGA
                     DAFSQISNWNNPEELLKLCTFVAASRPGYRLLGAEKKYPGRVKTIEVSALAISSSEIR
                     WRVKRGESIKYLVPETVENYIYKNGLYKD"
     gene            complement(11194..12444)
                     /locus_tag="PK016_00955"
     CDS             complement(11194..12444)
                     /locus_tag="PK016_00955"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HXL01681.1"
                     /translation="MGEKVKDIARKTKEASYFLSNVSSFYKDQFLLRLADNLERDEEE
                     IKKENRRDLERAKDMGMKPSLLDRLELSSSRIKAMASGLREVANLPDPVGEVIEGSKR
                     PNGLLITKMRVPLGVVAIIYEARPNVTIDSIGLGIKSGNALLLRGSSSAIYSNRVLVN
                     KTKETLSQCGFPPEVVNLLECESHEEVQELLTLRNYIDVVIPRGGAGLIKSVVENSQV
                     PVIETGVGNCHVYVDESADGEMAEKIVINAKTQRPSVCNACETLLVDEKIAPQFLPRM
                     VRVLREKGVEIRGCEKTKELVKEVKLATEEDWYAEYLDLILAVKVVGGIDEAIKHINK
                     YGSHHSEAIVTRDYAAARKFLEEVDAAAVYVNASTRFTDGGEFGMGAEIGISTQKLHA
                     RGPMGLRELTTVKYMVWGTGQIRE"
     gene            complement(12446..13576)
                     /gene="proB"
                     /locus_tag="PK016_00960"
     CDS             complement(12446..13576)
                     /gene="proB"
                     /locus_tag="PK016_00960"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HXL01682.1"
                     /translation="MSREEFQKNSNLIVIKVGSSLLVREGVLQLEKIKELACNIAYLR
                     ERGKKIILVSSGAVACGMSVMGLSRKRGDIPFKQAMSAIGQSVLMQAYREVFSPLGIQ
                     VAQILLAPEDTNNRVKYLNARNTFEALLHLGVLPVVNENDSVAVAEIKFGDNDRLSAL
                     VASLIDADLLVILSDVEGLFTADPRVLPQASLIEEVKKIDLEIEEMAGGEGNYFSTGG
                     MKSKIIAARMATLSGTGVIIASGKDFSVLQEIGEGKKRGTFFWPEVKHLPGRKRWIAF
                     AMMPRGNVLIDKGAKKAILTQKSLLPAGVKAVEGNFDLGDCVEILGPARELVGRGITN
                     YSSEELKKILGLHTEEVKEVLGGKDFDEEVVHADNLVLLGGD"
     gene            complement(13569..14837)
                     /gene="obgE"
                     /locus_tag="PK016_00965"
     CDS             complement(13569..14837)
                     /gene="obgE"
                     /locus_tag="PK016_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HXL01683.1"
                     /translation="MFVDSVIIRVQGGRGGDGAISFRREKYIPRGGPDGGKGGEGGRV
                     FLRASPHRETLRDLSFSPSYQASSGEGGKGKKQEGKKGEDLVIEVPLGTQVFDNRTGE
                     LLADLLYPGMEVMIARGGKGGKGNVFFTSPDNRTPRMAQKGEEGESRELRLELKFLTD
                     VGLVGLPNAGKSTFLSRVSNAKSLVASYPFSTSEPILGTVGEGEEKFILLDTPAIVEG
                     ASEGKGLGNRFLRHLERAHLLLYLIDLSSPFSEISPEEDLRIIREELRKYNSHLEDKP
                     FLVVANKLDLPEARENWNSFKAILERKKIEGWGISAHSGEGVSELLERIREILKEERK
                     KEVEIVAGEEKKERDISPVVYRFSSTYLERLLQEADLHSGEEEEFLNRELEKSGFKRY
                     LLPLKSSSIIEVGERKLVWKEKRLKILADE"
     gene            complement(14853..15113)
                     /gene="rpmA"
                     /locus_tag="PK016_00970"
     CDS             complement(14853..15113)
                     /gene="rpmA"
                     /locus_tag="PK016_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HXL01684.1"
                     /translation="MAHKKAGGSARNGRDSNSKRLGVKVYGGQFVKAGGIIVRQRGTK
                     IHPGVNVGRGKDDTLFALRNGYVVFEDKKNKKQVSVFSENMN"
     gene            complement(15118..15432)
                     /gene="rplU"
                     /locus_tag="PK016_00975"
     CDS             complement(15118..15432)
                     /gene="rplU"
                     /locus_tag="PK016_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013920104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HXL01685.1"
                     /translation="MFAIIEAQGKQYTVQEGSIIKLDSWLPREEEGVVFDRVLMWKKD
                     DALEVGKPYVEGAKVTGRVLRRDKGKKVVVFKYKPKVNYRKKTGHRQPLTLVRIEKIE
                     GE"
     gene            complement(15487..16968)
                     /locus_tag="PK016_00980"
     CDS             complement(15487..16968)
                     /locus_tag="PK016_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HXL01686.1"
                     /translation="MKKEIVIDMSEIETRAALLEEGKVVEFFFDRATQSRLVGNVFKG
                     VVENVLPGIQSAFVNIGLDKNAFLFIGDMLLDDKNKVQRVEELVRPGEEIVVQVTKEP
                     MGTKGARISTKISLPGRYVVLMPGIDTVGVSHRIGSAEERERLKKVLERLRPVSVGII
                     ARTAAEGKKSEELKDDIDNLLRLWDRILKKAEIMSPPCALYEEASLIYTLVRDVMDEE
                     VETVWINSFFGYQKLRDFVDSIAPSLSNRIKLFESRENIFEHFGLNREIEKALSRRVW
                     LKSGGYLVFDRTEAMTVVDVNTGKFVGKGNLQETIFKTNMEAAEEIARQVRLRDIGGI
                     ILVDFIDMEKKEHRRQVVRKLEEVLLKDRSRCTVVEMSELGLVEMTRKRLIKEIDSFL
                     REPCSFCEGEGKILSLETIAVNFLRKLEEICSNTSSPTVAFSVGEELGAYLQKEGQKF
                     LDNLKKLYSKEILWKIDPSLAPRRYAIIGVGSEEVREKMEKVI"
     gene            complement(16985..17557)
                     /locus_tag="PK016_00985"
     CDS             complement(16985..17557)
                     /locus_tag="PK016_00985"
                     /inference="COORDINATES: protein motif:HMM:NF021610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HXL01687.1"
                     /translation="MDRFRYRVKHQKMAPFHFISQLDLSRLWSRAFRRAGLPVVYSQG
                     FNPRPLLSFGPALPLGVESRAEYWDVSLSRELFPEELLNTLNGEVFPELKIEEVTPLP
                     PSFPSINRSMKGIRYSYYFSPSLKERASSLTEEMEIEEEKRELIGEFLVVFFLFKKEK
                     ILYSPGEWAKILEKEWGEDPTRIVKEEVLW"
     gene            complement(17559..19340)
                     /locus_tag="PK016_00990"
     CDS             complement(17559..19340)
                     /locus_tag="PK016_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HXL01688.1"
                     /translation="MDLEKILSRQEMLSISKPARYIGREWNMSMKKEEDVEISVALAF
                     PDLYEIGMSHLGLKILYHILNSLPGVRAERVFAPWPDMGKMMKEKNIPLYSLESKIPL
                     RSFDVVGMSLQHELNYTNVLYLLDLAGIPLWSEERGEEFPLILGGGPCAFNPEPVSSF
                     FDVFLLGEGEEAIVEIVEVLKAMKSEKRDNVLSELSKIEGCYVPRFYSVSYHPDGKIK
                     SISPGVVRKRWIKDLDSAPYPQKIIVPYLNIVHDRIPLEISRGCTRGCRFCQAGMIYR
                     PHRERSPEKVIELAEKLVKSTGYEEVSLLSLSSTDYSAIEYLVRELSFRLKESKVNVS
                     LPSLRMDAFSVGIAQSLQEVRRSGLTFAPEAGSERLRRVINKGLTDEDIFQTVGKTFS
                     SGWREVKLYFMIGLPGEREEDIRGIANLVKEILSRGKKRGVHLGISAFIPKSHTPFQW
                     AKEEEIESLEEKMAILHSLLRPLGHRVRYNWSDFEVNLIEGALSRGDRRISSVIERVY
                     RKGGVMNNWREFFSLQRWQEAFEEEGLNLNFYTHREREREEIFPWEHLSSGVNKDFLW
                     KEYQLSQEEKITPPCREGENCYNCGVC"
     gene            complement(19331..20419)
                     /locus_tag="PK016_00995"
     CDS             complement(19331..20419)
                     /locus_tag="PK016_00995"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HXL01689.1"
                     /translation="MEDKALILILVALSLIGVLAVYSVDPLRGEEWGASLFSKQIIWN
                     FVGWLVFLFFSQIDYHQWVKRGWLWYLLMVLSLGAVLVFGKGDETGSRRWLFSQSVQP
                     SEGAKIALALFLAHYFSEQRENFDFWPLLLKSGILSFIPLVLIFEEPDLGSSLVLLVI
                     WLGGLFVCGFSAKKWAVIGGIGVGLLPLSWFFLEDYQKARLLSFIDPGKDPLGSGYHI
                     LQSKIAIGAGGFTGQGWLSGTQSQLYFLPARHTDFIFASWCEQLGFLGGMVIIFLLGA
                     LSYLFLVIALRSPDLEGKILASLLGISLLFQWAVNIGMNLGVMPVTGIALPFISYGGS
                     SLVANLASAGLVYNISKSGGKESWKLWI"
     gene            complement(20409..22163)
                     /gene="mrdA"
                     /locus_tag="PK016_01000"
     CDS             complement(20409..22163)
                     /gene="mrdA"
                     /locus_tag="PK016_01000"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HXL01690.1"
                     /translation="MWNDEMQEQVNQRITYLSRLFLIILLILSFRFWYLSILQGDWYK
                     QKSEQNRVRVFSLSPIRGSIYDRNSQVLAEDIPRFRLIYEKGGMEVEEIEKRVERILG
                     RELSPPFPERGVGEIILIDPLSLEEAIKIEEAQNDLPGVRVETYPVRHYPRGEPFAHV
                     VGYVGKINAEELESLYSEGYEMEDRIGKVGVEREYESLLRGEKGYRRIEVDALGRVIR
                     VLDYRSPYFKNSVTLTLDGELQEYSYELLGNRNGVIIVGDPYTGELLALVSKPGFDPQ
                     LLSWGMTAEEWNTLSQNQDKPLTNRSLQALYPPGSLFKLVIATGALEEGLTDGNRKIF
                     CPGFLDYNGWRYPCWRRGGHGAVSIEEAIAQSCNVTFYTLGLELGADRIINWSGRLGV
                     GRPSGIDLPGEKTAFLPTPEWKKRQLKEMWYPGDTINLSIGQGYLLVTPLEMYRVVSS
                     IATRGEVYQPHVLKEVLDSKGEVIRKVSPVLQDRIELKNSTWETLIRGMEKVVNRGTG
                     QVCRGLPVELAAKTGTAQNPQGEDHSWFAGFFPSSHPQVVFLVLIEHGGDGSGEAAQT
                     AKKLIEWWEENRGGKSGR"
     gene            complement(22153..22641)
                     /locus_tag="PK016_01005"
     CDS             complement(22153..22641)
                     /locus_tag="PK016_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01691.1"
                     /translation="MIEKIGIFFFLPLLWEGISSSFLVHLWFPLRHLWIFSFLMVSLF
                     WEEREDNLDVLLIVILGITLDLLFFSPWGAYLFILSSLALFTYCWAKFFSTTSISLLA
                     IFFCFPLVELILVEIVAAIEGVTLPFFPLLGAKLLSIPLNIFLFYWWRRKEINRGLKY
                     VE"
     gene            complement(22638..23453)
                     /gene="mreC"
                     /locus_tag="PK016_01010"
     CDS             complement(22638..23453)
                     /gene="mreC"
                     /locus_tag="PK016_01010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HXL01692.1"
                     /translation="MNGNRSQKLLILIVLIVVSSQILIWAVRTEDNQIENIRSFFLPL
                     HRAGERVKDLFQGWGRFFSDSRTLGEENQKLKEEIDKLRAENILLQEKVVSLKMKIEA
                     GAMEEELPFEVISAQVIGRDPYDWWGKAIIDQGEKEEIVEGLIVVTYQGMVGKIEEVY
                     PHHAVIRLLLSPFLSVGALVQRTRDLGVVTGNGEGLCLMKYIYRTSDLREGDIVITSG
                     GEENTPRGIIVGRVSQIEEDDGSLFKNVIVEPSCDFSLLDKVFVIKALPEKLE"
     gene            complement(23456..24412)
                     /locus_tag="PK016_01015"
     CDS             complement(23456..24412)
                     /locus_tag="PK016_01015"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HXL01693.1"
                     /translation="MKHKRTLLVFLLFAFLFWGFFFRDKEEKKTFFGFDTWIEVELPR
                     DNVSLFSPISSLVNHLDQLWNRFSPQSIIFKINHSSLPLSVDEDTFRVLEKAQKGEEE
                     TQGFFNVLIAPLMDLWGFSEQPSLPEEREIKKIVGEINSSKLYLNRENREVLIEGEGQ
                     IDLGGIAKGYLIDEIVSLLRKEKVPQALINAGGEIFVLGKETKVGIRHPRQEKLLGYV
                     VIKNEAVSTSGDYYRFFEEGGVRYHHILNPFTGYPGRDFQAVTVISEEAVFADILSTA
                     IMAGGKEALAVVEKKFPEVKILTIEEDKIYLSSSMEKVFFAE"
     gene            complement(24615..25610)
                     /gene="amrS"
                     /locus_tag="PK016_01020"
     CDS             complement(24615..25610)
                     /gene="amrS"
                     /locus_tag="PK016_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013337645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HXL01694.1"
                     /translation="MKIARFFSSLENGRVQCFLCSHRCIIAAGRSGTCRVRRNEEGIL
                     YAQTYGKISSLALDPVEKKPLYHFYPGREILSVGSVGCNLHCSFCQNWSISQVSPADF
                     PLREINPEDLALLARENDSIGLSYTYNEPFIWWEFVYDTAQLVRKQGLKNVLVTNGYV
                     EEEPLRELLPWIDAMNVDLKSMDDDFYRHYCGGTLSPVLRTIEIAVREGLHVEVTNLI
                     IPGLNDRWELIEKLVNWIYDLNPDTPLHFSRYFPAYKLDNPPTSISFLEEVYHFAREK
                     LPFVYLGNTGGGKGNISFCPACGKEVVIRKGYQIDTSGLSGNHCRFCGSSLPFYN"
     gene            complement(25607..26146)
                     /gene="amrA"
                     /locus_tag="PK016_01025"
     CDS             complement(25607..26146)
                     /gene="amrA"
                     /locus_tag="PK016_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HXL01695.1"
                     /translation="MEEKSELGKIITSLARKSIEYYLEEGRYLPLPSGLPPYLLNERR
                     GVFVSLKKRGNLRGCIGTYIPQYENIAQEIIHNAVSAATQDPRFPPLQEEELPLLRIS
                     VDILSYPERVNSLEELDPEKYGVIVERGWRKGLLLPNIEGVDTVEEQIRIALAKAGIS
                     PQEKFTIYCFTVDRYEEED"
     gene            complement(26130..26576)
                     /locus_tag="PK016_01030"
     CDS             complement(26130..26576)
                     /locus_tag="PK016_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pre-16S rRNA-processing nuclease YqgF"
                     /protein_id="HXL01696.1"
                     /translation="MDFTGDHQSEKTIFSIDPGREKVGVAILSLQGEVLFHDILPEGE
                     IEREINILREKYRPFQVVVGNSTGREKIMKILEKMNLSFTLVDEHNSSEEARKLYFQA
                     HPPRGWRKIVPLSFLYPDEAFDDWQAVVIGRRYLNQRKSEENGREK"
     gene            complement(27059..28825)
                     /locus_tag="PK016_01035"
     CDS             complement(27059..28825)
                     /locus_tag="PK016_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HXL01697.1"
                     /translation="MAKVAEEMKEEMGQKKLVTIYLDGREVKVPEGVSVLEACNMEGI
                     KIPTLCYLEGLTPWGGCRICVVEVEGQPNLVASCVTAVREGMKIRTSSKKVREARKTN
                     LELLLSNHPLDCQLCDRNGSCELQDLSYQFGVREIRFPGEKKTYEVDRSSPSVKRDAN
                     RCILCGRCIRACQEIQTVFAIDFANRGFDSLVSPSLGLPLGESVCVNCGQCILSCPTG
                     ALSEVSHVEMVWEALEDPEKFVVLQTAPAIRVSIGEPFGLPPGTVTTGKMVAAFRRLG
                     FDRIFDTNFAADLTIIEEGTEFINRLQKGGKLPLITSCSPGWIKFMEHFYPELMENVS
                     TCKSPQQMFGAVAKTYYAQKLGIDPQDMVVVSIMPCTAKKFECQREEMKSSGCQDVDY
                     SLTTREAARMLKEKGIDLREMPEEEFDDPLGISTGAAAVFGATGGVMEAALRSVYEIV
                     SGKTLPSIDFYDVRGMEGVKEATVNVDGLEVNVAVAHSLGNARKILDRVKEGNSKYHF
                     IEIMACPGGCIGGGGQPIPTNLEIRMKRIEAIYQVDKNLPLRKSHENPAIKQLYEEFL
                     GEPNGEKAHHLLHTHYLAREKM"
     gene            complement(28833..30479)
                     /gene="nuoF"
                     /locus_tag="PK016_01040"
     CDS             complement(28833..30479)
                     /gene="nuoF"
                     /locus_tag="PK016_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HXL01698.1"
                     /translation="MFYRSHVLVEMTSNSLVRGAAQIKERLEREVERLGLDKEIKVLE
                     TGSLGAALPSPFVVVYPDNIIYAPVELSDVKRIVEEHFWKGRPVKDLIFSPSGRGEAG
                     AFLPYFPLSKEKRVVLRNSGQIDPTSIEEYIARDGYLALSRAISEMTPDEVIAEVKKS
                     GLVGRGGAGFPTGLKWELTRKAEGEEKYIVCNADEGEPGTFKDRLILEGDPHSIIEAM
                     AIAGYATGSQRGYIYVRGEYPQSVNHLKIAIDQAREYGLLGENILNSGFNFEVSIRKG
                     AGAYICGEETALLESIEGKRGIPRNKPPYPTQGGLWGKPTVINNVETLANIPPIILRG
                     ADWFRSIGTPTCPGTKVFSLTGNVNNWGLIEVPMGITLRELIFEVGGGIREGKGLKLV
                     QTGGPSGGTMTPDLLDVPMAFDTLSRYDSALGSGALTVIDDTHCIVDVVRNFMEFFLH
                     ESCGKCTPCRVGTKRIVEILNKISEGRGDLSDLENLRYMGEHIKSTSFCGLGQAAPNP
                     LLRSLSFFEEEFRAHIEEKRCPVDTCSMKTKTKNLIGVRS"
     gene            complement(30490..30954)
                     /gene="nuoE"
                     /locus_tag="PK016_01045"
     CDS             complement(30490..30954)
                     /gene="nuoE"
                     /locus_tag="PK016_01045"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HXL01699.1"
                     /translation="MTELAVYEEIDEIIDKYQEEKTPLLSILQEIQEKWRYLPGDVLG
                     YVSRKLNVPSSVVWGVATFYSFLETKPVGEYVIRVCRSAPCHVNGSLDVLKTLQKELG
                     IREGETSRDGKFTLETASCLGVCGVAPAMMINDVTYGNLSEDRIREVLALYR"
     gene            complement(30951..34034)
                     /gene="nuoF"
                     /locus_tag="PK016_01050"
     CDS             complement(30951..34034)
                     /gene="nuoF"
                     /locus_tag="PK016_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HXL01700.1"
                     /translation="MAEQERCQILLCAGAACISSGAVKIKEALIRELQKNALDNEVRL
                     IETGCVGPCNLGPLAIIYPEGVFYQKLTPEDAQEIVEEHLLKGRVVERLLYQPPQEER
                     KRFLREIDFFDKQVKIALRNCGFINPLNIEEYIARDGYLALSRAISEMTPDEVIAEVK
                     KSGLRGRGGAGFPTGLKWELTRKAPGHPKFIICNADEGDPGAFMDRSILEGDPHSVIE
                     AMTIAGYAVGAEKGYVYVRAEYPLAVERLGYAIDQAREYGLLGENILNSGFNFDVEIR
                     IGAGAFVCGEETALIASVEGKRGMPRPKPPFPAQEGLWGKPTVINNVETYANIPPIIF
                     RGADWFRSIGAENSKGTKVFALAGDVNNTGLVEVPMGISLGEVIYDLGGGIRGGKKLK
                     AVQIGGPSGGCIPVEHLNIKIDYDSLKELGAIMGSGGLIVMDEDTCMVDLARFFLEFV
                     QDESCGKCTPCRVGTKRMLEILQRITQGEGKEGDIERLEDLANQIKTTALCGLGQTAP
                     NPVLSTIRYFRDEYEAHIREKKCPAVVCRPLFVAPCQHACPLGINIPGYVSCIREGDY
                     LAALQIIREDNPLPSVCGRVCHRPCESKCRRGDMDEPVAIDNLKRFVADYARQNRIAL
                     PVVMDRKRDESVAIVGSGPAGLTCAYYLARLGYSVTIFEALPVAGGMLAAGIPPYRLP
                     REALEYDINQIKAMGVKIELNKAVGKEIEWKEVREKFDAIFLGIGAWKSAPLGIPGEN
                     LSGVVSALDFLRDFNLGKQIEVPRKAVVVGGGNAAMDAARTLLRLGAEVNVVYRRTRG
                     EMPAIPEEIEDAEKEGVIFHFLMAPLEVVGENGKVKGIRLQRMKLGEFDRSGRRRPVP
                     IEGAEALWECDMVVEAVGQIPEVENLGEMLTLDHRGNILVDRYSLSTSIPGVFAGGDA
                     IGKEATVVDAMALGKRAAFNIHQYLRDLKPEEEEVIIQPERPKVLEEPPVLQEIPRVK
                     PPELPVRERVCSFSEVKLCLEEEDARREAERCLRCDLEKILKKYQEIGMAEEEVQ"
     gene            complement(34040..34420)
                     /locus_tag="PK016_01055"
     CDS             complement(34040..34420)
                     /locus_tag="PK016_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HXL01701.1"
                     /translation="MKVNSIEDLKRIKEEAQKRTLLREGKADYKVVVHMGTCGIAAGA
                     REVMSAILDEIAKRGLTNVMVTQAGCIGLCDREPLVSVEKGEERVLYGDLTPDKARQI
                     IASHLVNGQIVGDWVVHAESRARR"
     gene            complement(34407..34967)
                     /locus_tag="PK016_01060"
     CDS             complement(34407..34967)
                     /locus_tag="PK016_01060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXL01702.1"
                     /translation="MKELSLHILDVVENSLRAGATKVRISILEDTRENRLELVIEDNG
                     EGMDAETLKMVRDPFFSGQGKKVGMGIPLLDQLAKQCQGDLEILSEKGKGTTVRATFQ
                     RDSLDLPPLGKLADTLFVIMASHPEVELEFIHRINDCQWLFNSKKMEKELDLTGENRS
                     VLLPAIREWIEYQEEILKGGMENESK"
     gene            complement(34971..35462)
                     /locus_tag="PK016_01065"
     CDS             complement(34971..35462)
                     /locus_tag="PK016_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HXL01703.1"
                     /translation="MADLLAEKEAISPEQEEALEVILQKFKGKPGSLIQALHAVQNLI
                     GYLPPPVLKKVSHTLDIPLSEIYGVVTFYHFFSLKPRGKHLIQVCLGTACYVRGGQEI
                     LNRLVQDLEIGVGEVTEDRLYSLEVMRCAGACGLAPVVKIDNDIYKRVNPAQITEIVK
                     SYS"
     gene            35656..36201
                     /locus_tag="PK016_01070"
     CDS             35656..36201
                     /locus_tag="PK016_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HXL01704.1"
                     /translation="MLETLIIAIGLGLDAFSLATAFGMCQKTCPQDAKLRLSFSFGAF
                     QFFMPLVGFGIGSQVAQLISSFDHWVVLGTLSLVGGKMIYEGWKRDDDTPFVDFSQGW
                     PLLFASLATSIDALMVGFSFALMDKNIWFSALIIGIVALLMTYLGVSLGHKIRGIFSS
                     PEVVGGIAIVLVGLKIFLEDL"
     gene            36217..>36963
                     /locus_tag="PK016_01075"
     CDS             36217..>36963
                     /locus_tag="PK016_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXL01705.1"
                     /translation="MAKGCIFGPILSRRLGSSLGINLLPYKTCSLNCIYCECGATTNL
                     TIERKEYIPTQEVMESLKKYAASSRFRSAPPFCITFAGWGEPTLHSSLGEIAHFIKQL
                     FPEQKLVLITNGTLFGFHEELLEEVREMDIIIPSLDAASSQAFQIIDRPHPQITLDSY
                     VEGLVKLRKHFRGEIWLEIFIIEGINDSEKELNFLKEKIALISPHRVQINALDRRPAQ
                     EGIKSPPLYRLEEIASFLGGEVILPLPKQSK"
BASE COUNT        10823 a         9151 c         6071 g        10818 t
ORIGIN      
        1 cccccaaaaa gcagacagcc tcttagctca gtatcctact ttacagggtt tctattgggc
       61 aaaagagaaa attagagaac tatatcggca aaaggataga gaagaagctg ctaaactttt
      121 agataacatc atctttaatc ttaaaatagc cgatgatgct gaattagtta gatggggaaa
      181 tactctaaag aaatggagag aacctatttt aaattatttc cataatggaa ctactaatgg
      241 ctatactaaa atattgtaat actaagatta aaatgctaaa gaggatattg ggggttgagg
      301 aatgtagagg tatactggag aaagatgttg ctgggattca taccaagaag agaatgtttc
      361 cccacctatt gacatagagc cagtctatac ttgccttctt acattctttc aagttatgat
      421 agctcaaaca ttcattatat gataaatttt atcgttttcc cataattggc gactatcgcg
      481 cctttagtat tgcttaagcc ggggtcacga gtttcccctc ctggataaaa gtgttagatg
      541 gtcacttgca caaataaaaa attgaggagc ctgatgccga agatgaatcc cattaatttt
      601 gagcagaaca aaaggttaga aaaacagatt aacttcataa tcgagataga taaagtcaaa
      661 aacatcattc gaaaatcgct attatttgat ggtagtcgat ttgaaaatgt ggcagaacat
      721 tcctggacta tatctataat ggcagttcta ctagaagaac atgccaactt caaagtcgat
      781 acaggaaaag tacaatttat gttattaatt catgatatcg tagaagctga tgctggtgat
      841 acatttttat attctgaaga taggaaaaat gctcacatta atgaagaaaa ggcggcagaa
      901 agaatattcg ggatgctgga accagaccag aaaaagatat tccttgatac ctggaaagaa
      961 tatgaagaag gaaagacaaa cgaagctaaa tttgctttag tattcgatag actacagccc
     1021 attattcaaa attacgtgaa caaaggtttc acctggaaaa agcataatgt cacttacgaa
     1081 caagtggtaa aagaaaacaa acacatcaaa gaaggctcag aagaaatatg gagttttgta
     1141 gagcacattt tacaggaatc ggtggaaaaa ggttacttaa ctaaaaacca atctcttgaa
     1201 gaggaaaaga aataaactat tagaaagaga atccaatcgc gcattagaaa ctacgggatt
     1261 gctaacgggt aaccctcaca agctgccctg tagcaatcca gaagcaagat taccacacac
     1321 gccgggatat cccggcgtgg ttttttatct tcatcaaatt ttatcaatgc catgtcgctt
     1381 cagccaatct cgaaaactct ggtcgtcttc taaaatttcc cggtcataat tctcctctaa
     1441 tttcttaatt cgctctacta agtcaggata gttctttact agcttttcta cgcttctatc
     1501 aaattcttcc ctcatatttt ccagatcttc tcctaccaaa ggcagattca gaagagaaga
     1561 aaggattctt aaaacagaag aaatccccct ggggttagta gcctggatat acataggtat
     1621 ttctaccact aagttaatca tttctatacc tctgtgggaa gccatgaggg taagaagagt
     1681 ggtaatgctg gctggacctt cataatcatt gaaacgaaaa ttataatctt tcagttcttc
     1741 ttttaatttc tcatcagaaa ctgaacaact tacccttggt tctcgagtat gaggaatcaa
     1801 cccactaaca ctacctatga agtaaatcct ctttactcca aattctttcc ccagagcaaa
     1861 gatattctct gcatactcat tccacttcat gttaggttct ttgcccagaa aaagaagaag
     1921 attttcctta gaagcacaga acaactggtt ctggggataa tgaaattcgg ttactattcc
     1981 ttctttaatc gaagtgtaag gacgaaattg agctacctcc tccatacttc caggaaaatt
     2041 aaacaggtaa aaatcctggg ggtcgatttc tgctatcatt tccgcagcaa atttcttctg
     2101 gagataggcc accgaaccac tggaaacatc tcctccatcc atccatccgg taaaccctat
     2161 taaaagagaa ggatttgata ataaaggcct ttgatatata gtaagcttgt ccatttccca
     2221 ccctcccatt ttcaatattc tgttgaaccc cggagcgttc tttaactaag gagggggtct
     2281 aaagagggaa accgctccga ggttcaacct ttttatattt aaagtttaat aaatagttcc
     2341 ttccccaact tatttttttt aaaaattatc gggccaggtt ttcaaaacga gcataatctt
     2401 tctggaacat taaatctacc gtccctatgg ggccgttgcg ctgcttggct acaatgattt
     2461 cggcaatccc ttttttagaa ctattaggat tataataatc ttcccgatag ataaggagaa
     2521 ctaaatcggc gtcctgttct atagctccgc tttcccgcaa atcagaaagc tgaggtcttt
     2581 taggagtcct ttgctctaca gctctactaa gttgagatac cgctataaca ggaacattta
     2641 attctctggc catagctttc agagagcgag agatttccga aatctcctgt tgccgatttt
     2701 ctggacgacc atatcctctc ataagctgga gataatcaac tataactaaa cctaaatcct
     2761 tttctgcctt aagacgtcgg gatttagccc gcaactccag aacagaaata ctgggagtgt
     2821 catcaataaa aatatttgct tcagccagtc ttcctgctgc tcgggctaac tccggccatt
     2881 ctctctccgc tataaacccc tttctcactt tagctgcgtc aattcgggct tcactgcaga
     2941 gcattctctg agcaatctgg tctttagaca tctccaggct aaaaatagcc accgggacat
     3001 tcttcttggt tcctacatat tgagcaatgt tcaagcacaa gctagtttta cccataccag
     3061 gacgggctgc taccaccaca aaatcggaag gctgaaaacc agcagtgatt aaatccaggt
     3121 ctacaaaacc agaaggaact ccggtaacat gctcatctcg atgatataat tcttcaatcc
     3181 ggtcaaaagc ctcgttaatt atagctttga gaggcacaaa atcatagcga attttctttt
     3241 gagaaagttg taagatccag ttctgggctc ggtctaaaag ctctttagcg tctagatctg
     3301 aattatagct ttcccggatg atccgggtac aaaggttaat caccgaacga aatatagatt
     3361 tctcttctac gatgtgagca taatactccg cattagcagc tgtagggaca atgtttacca
     3421 gctgggttac atagtccata ccccctacag cctccagctg gtttttttgc ctcaatttgg
     3481 ctaccagggt tacaatgtca caggcttggt cttcttcaaa aagttctaca atgctttcaa
     3541 aaataagacg atggttttcc ttataaaaat cttctggctg taaaatatcc aatacccgca
     3601 gaagtgcttc cttatcaaga agcatagacc ctaaagtagc ttgttccgcc tcgagatcat
     3661 gaggaggaac cctttcaata ctcaggactc ttccgcctcc tccggcactt cttctccctg
     3721 gatttttact tgaactgtag cataaacacc cttacccagg ttaactttca ccggatattc
     3781 acctatttct ttgagtgatt cttctaactc cagatatttt cggtctaaat ttatacccgc
     3841 aatccgagaa atttcctccg ctatctcccg ggaagtaacc gagccgtaaa gttttcctgt
     3901 tttaccagct tttttcttga attcaagaat caggccctct attttcccct gtaacgcttt
     3961 agcctcttct aactctttag cctcccggat ggctcgtttc ttttttagcg cttctatctg
     4021 ggtaatatta cccttattag cctctacagc tagttttcga ggcaaaagat agttgcgagc
     4081 ataaccactt tttacctcta ctacatctcc ttctcttccc aaattttcca cttcctgcaa
     4141 gagtatcact ttcatgttat atcctcctca cttacctcta attttccgca agtcaaacca
     4201 cacatcaaaa actcccaacc agctcaatat ggtagaaagc aaaggttgca aagctaaaaa
     4261 aatcactata atcgttaacc acactcgaga acgaatatac ttaccaagaa aatagtatac
     4321 cacggacgcc ccctgaacta aaaaaagtat ctgagtgacc atctgtaaat tcaaaccgat
     4381 cttgccccag aaagtagttc ctccaaatag aacgaaaagc catcccagag caaaagccca
     4441 gaaaatagat ttaggtagtt gccattcccc aaagggggga aaaggaggaa atttgatccc
     4501 caatctcttt cctatccagc cacctaaaag aaaattgagc accgtgtcca gaatagaagc
     4561 gacaattacc gtagcaggta aagccatgcg aataagattg attatatctt ctacatttat
     4621 gcctccctca gaagggagaa aagacaaacc ctttttcaaa ccttcttcta aaaaagataa
     4681 actttctgcc agaggattct gacccatgat taaaattact aaaccaatta ataacaattt
     4741 agaggagata gaaaccagag tatttattcc tattacttct ataaagttat aacctctttt
     4801 gatggtaagg cccataatta atcccaaaag agtaaatcct acaaaacagg tcagggcaga
     4861 tactactccg gcaaagataa aaactataaa actggccacc agagctgcca gtaaccccgc
     4921 tctcatatcc cagcgaatgg ttaagaacat aaccggaaga gggcagagaa aactcaataa
     4981 aaaacctaaa ataggaatat atatggaaga taaataaaaa acaacggtga gggcagctaa
     5041 aaaagctccc tctgccgtgg atctggcttg tctcattaat cctaatctat tgtataaggt
     5101 aaaagagcaa gttccctcga cctttttata gcctgggcaa ctttccgctg gtgtttagca
     5161 caagttcccg tgacccgacg aggaacaatc ttacctcttt cacttaaaaa tctctggaga
     5221 aaatcggcat ctttataatc tattgttgct tttcccaaac aaaacataca gggtttcctt
     5281 tttctgcgga agaatttctt ccgtcccgag tattcatcct tatctactgc cattttcagt
     5341 tacctcctct ctttttttat tctccggata tgataaaagc ttcactcctg aagctacaat
     5401 ttcataagct cttctttttt taccttccga gttttcatag gttcttgcct gaaccctccc
     5461 ctctacgaaa atccagtcac ctctttttaa attttctcca cagaaaagag ccaggtctcg
     5521 ccacgctatt atgttgaggt aatctatcca ttctttttct tcctcagaag ggatagtccg
     5581 atataccgcc agcgaaaagt taaccaccgg tatacccgag gggacatagc gcactaccgg
     5641 actatgaggt aaaaatccga ttaaaaaaac ccggttaaga ctgagatgag acatcaccag
     5701 ctgtttcttc gcttactatc tcttctggaa tcttctcttc tggggaagat gaagtagtag
     5761 aaacctcttc ttcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnttcatct
     5881 tctttaacta aaagatgacg aataactttc tcatctaaac ttatccatct tttaacttct
     5941 gctacttgag aagggggaag cttaaaatta aaaaatgcat aataaccctc ctggtacttt
     6001 tgaatagggt aagctaactt ccttcttccc cacaggtcta cctttaccac ctcgcccctt
     6061 tctcccagaa agttcttaag tttttctact tctgagctta attcttcttc agaaagagtg
     6121 ggccttaata ttactcccag ttcataacta ttcatgttca tgttaattcc tccctttgga
     6181 cttttttggt ttcacttttg gtgaaacagg gaaatttatt aagtgccttc ttgccaggaa
     6241 gaaagatatt ctctctggcg agaagtcaat tcttcaattt ctaaacccag agattgaagt
     6301 ttcaggaaag ctatctcccg gtctatttct tcgggaactc gataaactct tttctccaga
     6361 tacgaagcat tttccttcaa atatttaact gacagagcct gattggcaaa actcatatcc
     6421 ataactgtag gaggatggcc ttctgcacag gtcagattaa ccagacgtcc ttctcccaga
     6481 aggtatactc tttttccatt ctttaaagtg tattcctcca ccaaaggacg gacttccctt
     6541 ttgtttacac tcatttcttc cagctctttt atatcaattt ccacattaaa atgaccagcg
     6601 ttagctaaaa tagctccatc cggaagagat aaaaagtgtt ctcctcgaat aactgaggta
     6661 tttccagtag cagtgataaa aacttccccc caaggagcag cttttttcat ggataatacg
     6721 ttattcccat ccatcagtgc ctctagcgct cttacagggt ccacttctac cactgctacc
     6781 tgagctccca tgcctttagc tcgggcagca attcctctcc cacaccatcc ataacctgct
     6841 accactactt ttttacccgc caataaaata tgagtagctc gtaaaatagc ttcgatagta
     6901 ctttgccctg ttccataacg attatcaaaa aggtgcttgg taagagcatc atttacagca
     6961 atgaccggat aacccagttc tccatttttt tccagactcc tcagcctaat gactccagta
     7021 gttgtctctt ctgtccctcc tttaatttcg gaaagaagct ctcttttgtg gccatgcaca
     7081 aaggaaacca ggtctgctcc atcatccata gtaatatgag gcctaaactc cagtactttt
     7141 tgaatatgtt gataataagt atcgtgattc tctcctcgaa tagcaaaaac agaaatgccg
     7201 taatccttta ccagggaagc tgctacttca tcttgagtgc ttaaaggatt agaagcgcaa
     7261 agccgtattt ctgcacctcc tgcctgcaaa gccctcacta aaaccgcagt ttcagcagta
     7321 acatgaagac aggcagctac tctcaaccct ttaagaggca gggtatctct ccacgaagaa
     7381 gcaataccag taactactga catctcccgt tctgtccact caattttctt ttttccctcg
     7441 ttagctaaat ctaaatcagc tacttcgaat tccaccattt tccgcctcct ttcagagtcc
     7501 tgctttttcc cttaaaatat caactttatc agttttctcc cagctaaaat cagggtcgtt
     7561 gcgcccaaag tgaccataag cagcagtctt tttataaata ggtcgccgaa gtttgagatt
     7621 tttaattata gctccagggc gaaggtcaaa aacttccctc accaggcgaa gaatttcatc
     7681 atcaggaatt ttccccgttc ctttggtatc tatactcata gaaaccggac gggctacacc
     7741 tatagcataa gctaactgga attcacattt gctggccaga ccagcagcta caacattttt
     7801 ggccacatag cgagcggcat aactaccaga acggtctact ttagtggggt ctttaccaga
     7861 aaaacaccct cctccgtgtt tgctaatacc tccataggta tcaacaatga tttttcttcc
     7921 cgtaagacca gtgtcccctt gtggtccacc aatgacaaat cttcccgtgg ggttaactaa
     7981 aatctgggta ttatcatctt tataagcagg aggaataatc gggtttatta cctctctcac
     8041 tatatcttcc cgcagagtat ctaaagaaat atcaggatga tgctgagcag aaaccactat
     8101 attggcaatc cttaccggtt tatcgtctac ataatccaca gtaacctgcg tttttccatc
     8161 aggtctcagg taaggaagaa tctccttttt cctcacttcc gccagacgaa aagctaaacg
     8221 gtgagccaga atgataggaa aaggcataag ctccggagtt tcatcacaag cataaccata
     8281 catcataccc tggtctccag cacctaaaga aagttcttca tcttccactt cttctcccat
     8341 ttttacttcc agtgaacgat taactcctaa ggcaatgtct ggagattgct catctatagc
     8401 agttaaaact gcacaggttt caaaatcgaa accatattta gctcgagtat aacctatatc
     8461 ccttactgta cttcgggcaa tatgagggat atctacataa gaattagtgc ttacctgccc
     8521 ggcaattaaa attaaacccg ttgctaccag agtttctact gctacccttc cttgagggtc
     8581 ttgagctaat atagcatcca gtatagcatc agaaacctga tccgagattt tatctggatg
     8641 accttcagta actgattcag aagtaatttg atatcgtttc tccaatgtaa tattacctcc
     8701 cttccatcgc cagaaaagat tttataatta attcctcttt caaagaactc attcatcaaa
     8761 aaccaaactt ttacattaaa tcataataga gaaagaaatt caattaatct tctccaaaag
     8821 atttaaacct tctttttctg aataaagaag gggagctcta tgccacaacc tttccagttg
     8881 ataaaacagt cgttctcctt ctcgaaaaat atgaatcatt aaatcaccat agtcaataag
     8941 gatccattct ccttcttcca tcccctcaag atgagaaggc cgcaattggc gctcttccat
     9001 ctttttcagg acttcttctg ctactgcttt agtctgaaga gcagaagaac cactacaaat
     9061 gatccagtaa tcagaaaagg gaaatattcc tcgtaaatca agaacggcga ggtcaaaagc
     9121 ttttttactc tccgccgcct ccttggctaa tactatcttc tcctgaattc tcaatatcct
     9181 ccctccgagc aaagaaaaat ttctgaacca tctcttgaga agcctcgagg tctggttccc
     9241 aatagcttac tccatttatg tatgttggtt ccccgggcat cgtttccatt cttattatcc
     9301 gttcactcaa tccttgaaac cacataacca actgggtgag gtagtcaccg gtcaaattag
     9361 tagttagagc ttcccgggag ctttctatca attgaggaat tcgagtcacc aaaagagggt
     9421 cattctctac tttatccaaa agagcctgaa ggaatttttg ttgtcttctg attcttccaa
     9481 tatctcccag cgcgtcatat ctaaagcgga catactggag cgccttttct ccattgagca
     9541 gttgtttccc tgcaggaatg tctatataaa gaccttgcgc tttgtccaca tacttaagag
     9601 gtttctctac ttctatttct atccctccta aagcatcaat tactttttca aatccttcat
     9661 aatccacttc cacaaaatag ggaattttta tccccagagc actttctaaa gtttgccgga
     9721 gaagccctat tcctccttga gcataagaat gattaacttt atcatatcct cttcccggta
     9781 tcaaaagacg agaatcccgg ggaacggaaa aaagaaggac ctcatctatc tgggaattaa
     9841 tacctactag aattatagta tcgctccgtc gaggttcaat actatcctga cccaccacta
     9901 aaataaaagt atttggtggt tcactgtaac ccattacttc ttttactacg gtccagcgta
     9961 aagggattct gaaccctaaa agagtggata aaaaaagaat aaaaacacct aaaaaaaatc
    10021 ccagaagaat ataaaaaacc tttcggcttc ttcttttatt tttcattttc tttgttccag
    10081 gctttccagg cagcagaata aactagaaaa ttatgagaaa gaagaaaaga taatttttct
    10141 tttaaaacct gccggtaagc ttcctcccag tctttttctg ccatccctct tattaaattg
    10201 gcttcaggaa attcccgccc cgcttcacaa aaatcggcaa tgaataaaat tttatcaaat
    10261 ctggacattg cctcattcga ggtagcatgc cagcgtatag cgtgccagat ttgatagtct
    10321 ttaactccaa attctctttc tactatcgca ggccctgcta aagcatggaa aatagcagga
    10381 atagaatagg cttcttcttc caggtaagga ggtaaaagag gaactaattc ctcagaagat
    10441 aaatctcggg cacagtcatg tagaagaccg gcaacataca atttatcttc cctttctccc
    10501 cagcgagaag ccaaatattg agcttcttct gccactccca aacaatgata aaatcgagcc
    10561 tcggtaagca tatttttcaa agtaagaatt atttggtctt taatctttat ataatccatt
    10621 tttgtaaata taattctcca ccgtctccgg taccaggtat ttaattgatt cgcctctttt
    10681 tactctccat cgtatctcag aagatgaaat agctaaagcg gagacctcga tagttttcac
    10741 cctccccggg tatttctttt ccgctcctaa caatcgatat ccgggacgag aagcagccac
    10801 aaaggtgcag agtttcaaca gctcttccgg attattccag ttggaaattt gagaaaaagc
    10861 gtcagctccg gtaataaaaa aaatctccgt cccctctccc cataaattct taaagtaacg
    10921 aactgtatct atagaataag aaggaccagg cctttctatc tcaacccgag aaacctgaaa
    10981 atagggattg gtaaccgtag ctaaaacagc cataaggtag cgatgttcag ggtcgcttac
    11041 ttcttgtcct attttgtgag gagggcgagc agaaggaaca aaaaccactt cgtctaaatc
    11101 aaagtagtct cttacctcct cagcagttac taaatgccca tgatgaatag ggtcaaaagt
    11161 tcctcccata attccttttt taggcgtaaa atatcactcc cgtatctgtc ctgttcccca
    11221 gaccatatat ttaaccgtag taagctctcg caaccccata ggacctcgag catggagctt
    11281 ttgagtgctt ataccaattt ctgctcccat tccaaattct cctccatcgg taaaacgagt
    11341 agaggcgttg acataaactg ctgcagcatc tacttcttcg agaaactttc tcgctgctgc
    11401 ataatcccga gttacgatgg cttcagagtg atgagaacca tatttattga tgtgttttat
    11461 agcctcatct attcccccca ccactttgac tgctaaaatc aaatctaaat attctgcata
    11521 ccagtcttct tccgttgcta attttacttc tttcactagc tcctttgttt tttcacaccc
    11581 tcttatttcc actccttttt cccttaaaac acgaaccatc cggggaagaa attggggagc
    11641 aattttttca tccacaagaa gagtctcaca ggcattgcaa acactggggc gctgagtttt
    11701 agcgttaatg actattttct ctgccatttc cccatcagca ctttcatcta catagacatg
    11761 gcaatttccc actcctgttt ctattaccgg aacttgagaa ttttctacca ccgattttat
    11821 caaacctgct cctccccggg gaataaccac atctatataa ttccggagag tcaaaagctc
    11881 ctgcacttct tcatggctct cacactctaa caaattaacc acctcaggcg ggaaaccaca
    11941 ttgggataaa gtctctttag ttttatttac cagcactcga ttagagtaaa tggctgagct
    12001 actacctcga agaaggagag cattacccga ttttataccc aaacctatac tatctatcgt
    12061 aacatttgga cgagcctcat aaataatagc tacaaccccc agaggcactc tcattttagt
    12121 aatcaaaagg ccattaggcc ttttagaacc ctctattact tctcccaccg ggtcaggaag
    12181 attagccact tcccgcaggc cagaagccat agcttttatg cgggaagaag ataattccag
    12241 acgatctaaa agagagggct tcattcccat atctttagct ctttctaaat ctcttctgtt
    12301 ctcttttttt atttcttctt catctctttc taagttatca gccagccgta aaagaaactg
    12361 gtctttataa aaagaagaaa cattactaag aaaataggaa gcttctttag ttttccgggc
    12421 gatatctttc accttttctc ccacactaat ctccccctaa tagtactaaa ttatcagcat
    12481 gaaccacttc ttcatcaaaa tctttccccc ccagaacttc ctttacttct tcagtgtgca
    12541 aacccaatat tttttttagc tcctctgaag aataattagt tattcctctt cccaccaatt
    12601 cccgagcagg acctaaaatc tctacacaat ctcctaagtc aaagtttccc tccactgcct
    12661 taactccagc aggaaggagg ctcttctgag tcaaaatagc ttttttagct cctttatcta
    12721 tcaaaacatt accccggggc atcatagcaa aagcaatcca cctcttcctt cccggaaggt
    12781 gtttcacttc cggccagaag aaagtgcccc gttttttccc ttctccgatt tcttgcaaaa
    12841 ctgagaaatc tttacctgaa gctattatca ctcctgtacc agagagagta gccattcgag
    12901 cagctattat tttactcttc attcctccgg tagaaaagta gttaccttcc cctccagcca
    12961 tctcctctat ctccaggtca atttttttca cttcctcaat caaagaagct tgaggtaaaa
    13021 cacgggggtc agcagtaaag agaccttcta catcagagag aatgaccaga aggtctgcat
    13081 ctattaaaga agctaccaga gctgaaagac ggtcattgtc accaaattta atttccgcca
    13141 ctgccactga gtcgttttca ttaaccacag ggagaactcc cagatggaga agagcttcaa
    13201 aggtattgcg ggcgtttaga tatttaactc gattattagt atcctcaggt gccagaagta
    13261 tctgagctac ctgtatcccc aagggagaga aaacttcacg gtaggcttgc attaaaacgc
    13321 tctgtccgat ggctgacata gcttgcttga aagggatatc tcctcgcttc ctggataacc
    13381 ccataacact cattccacaa gctactgctc ctgaagaaac cagaataatt tttttccctc
    13441 tttctcgcag ataggctatg ttacaagcca gctctttaat tttttctaat tgtagaactc
    13501 cttccctcac taaaagtgag gaacccactt ttatcactat caaattgcta tttttttgaa
    13561 attcttctct actcatcagc gagaattttc agcctctttt ccttccagac cagcttcctt
    13621 tcccctactt ctattatgct tgaggatttt agaggtaata aataacgctt aaaaccggac
    13681 ttttctaatt cccggtttaa aaactcctct tcttctccag agtggaggtc tgcctcctga
    13741 agcaatctct caagataagt agaagaaaaa cggtaaacca ctggagatat atctctttct
    13801 tttttctctt cccctgctac tatttctacc tctttttttc tttcctcttt taatatttcc
    13861 cttattctct ctagaagttc actcactccc tcaccggagt gagccgatat cccccatccc
    13921 tctattttct tcctttctaa aatcgcctta aagctattcc agttttctcg agcttcagga
    13981 aggtctaact tgttagccac tacaagaaat ggcttatcct caagatgtga attatatttt
    14041 cttagctcct ctcgtataat ccttaaatct tcctcaggac tgatttccga aaaaggagag
    14101 gataaatcaa tcaaatataa aagaagatgc gctctctcaa gatggcgaag aaatctattt
    14161 cccaggcctt ttccttcgct cgccccctct acaattgccg gggtatccag taaaataaat
    14221 ttctcctcac cttcacctac cgttcctaaa attggctccg aggtagaaaa aggataagaa
    14281 gccaccagag atttggcatt actcacccga gatagaaaag tagacttccc ggcattaggt
    14341 aaccccacca ggcccacatc agttaaaaac tttaattcca gacgaagctc tcgtgactct
    14401 ccttcctccc ccttttgtgc cattcgagga gttcggttat caggagaggt aaagaaaaca
    14461 tttccctttc ctccctttcc tccccgagca atcatcactt ccatccccgg ataaagaagg
    14521 tcagcaagca attctccggt gcggttatca aaaacctgtg ttcccaaggg aacttcaatt
    14581 accagatctt cacctttttt gccttcttgt ttttttccct tacctccctc tccagaagaa
    14641 gcctggtatg aaggagaaaa ggataaatct cgaagggtct cccggtgagg agaggcacgg
    14701 agaaaaactc ttcccccttc tcctcctttt cctccatctg gcccaccccg gggaatatat
    14761 ttttcccgac gaaagctaat cgccccatct cctccccggc ctccttgcac tctaataatt
    14821 acgctatcta caaacaataa ctctcctcac ttttaattca tattttcgga aaaaacagaa
    14881 acctgttttt tatttttctt atcttcaaaa accacatacc cattacggag agcaaaaagg
    14941 gtgtcatctt tccctctacc tacattgact cccggatgaa ttttagtacc tcgctggcga
    15001 acaataattc ctcccgcttt aacaaattgt ccaccgtaca ctttcactcc caaacgtttg
    15061 gagttactgt ctctaccgtt acgtgcactt cctcctgctt tcttatgagc catagtctca
    15121 ctctccttct attttttcaa tacgcaccaa agtaagaggc tgacgatgac cggttttttt
    15181 ccgataattc actttgggct tatatttaaa taccaccact tttttacctt tatctcttct
    15241 gagaactctc ccggttactt ttgccccttc tacatagggt tttcccacct ccagggcatc
    15301 gtcttttttc cacataagca cccggtcaaa aaccactcct tcctcttccc ggggtaacca
    15361 gctatccagc ttaattatac ttccttcctg cacggtgtac tgttttccct gcgcttcaat
    15421 aatagcaaac ataaatttca cctcccagag aacggtaaag aatttaccat agaaaccgga
    15481 atttagtcaa ataacttttt ccattttttc ccggacttct tcactgccca ccccgatgat
    15541 agcataccgg cgaggggcaa gagatgggtc tattttccat aatatttcct tactgtataa
    15601 tttttttaaa ttgtccagaa atttttgccc ctctttctgg agatacgctc ctaattcctc
    15661 tcccactgaa aacgccactg taggagaaga ggtattactg catatttcct ccagctttcg
    15721 taaaaaatta accgctatag tttccagaga taatattttc ccctccccct cacaaaaaga
    15781 acaaggctct cgtaaaaaag aatctatttc ttttatcagc ctttttctgg tcatttctac
    15841 cagccctaat tcactcatct ccaccactgt acagcggctt ctatctttca agagaacttc
    15901 ttctaatttc ctcaccacct gccggcgatg ctctttcttt tccatatcaa tgaaatcaac
    15961 aagaattata cctcctatgt ctcgcaacct tacctgacga gcaatttctt ctgctgcctc
    16021 catattggtt ttaaaaatgg tctcctgcag gttccctttt cccacaaatt taccggtgtt
    16081 cacatccaca acggtcatag cctcagtccg atcaaaaact aaatatcctc cacttttcag
    16141 ccacactctt ctactcagag ctttctctat ctcccggtta aggccaaagt gctcaaatat
    16201 attctcccgg ctttcaaaaa gcttaatccg gttacttaaa gaaggagcga tagaatctac
    16261 aaagtctctg agcttctggt aaccaaaaaa agaattaatc cagacagttt ccacttcttc
    16321 atccatcaca tcccgcacta aagtatagat gagactggct tcttcataaa gagcacaggg
    16381 aggagacatg atttcagcct tttttaaaat tctatcccac aaccgaagca agttgtctat
    16441 atcatccttc aattcttcag actttttccc ctctgctgcc gtacgtgcta ttatacctac
    16501 actcacaggc ctcagccttt ccagtacttt cttcaatctc tcccgctctt ctgcggatcc
    16561 tattcgatgg gaaaccccca ccgtatctat accaggcatt aaaactacat accttcccgg
    16621 taaagaaatt ttagtactta ttcgagctcc tttagttccc ataggttctt tagtcacctg
    16681 cactactatt tcctcaccgg ggcgtaccag ctcttccact ctctgcactt tatttttatc
    16741 atctaaaagc atgtcgccaa taaaaagaaa ggcattctta tccagaccaa tatttacaaa
    16801 agcagattga ataccgggaa gaacattctc taccactcct ttgaaaacat taccgaccaa
    16861 gcgagattga gtagctcgat cgaaaaaaaa ctctactact tttccctcct ccaagagagc
    16921 tgctcttgtc tctatctctg acatatcaat tacaatttct ttcttcaatt atccaacctc
    16981 cgttctacca taaaacttct tctttaacta tccttgtagg gtcttcaccc cactcctttt
    17041 ccagtatttt tgcccactcc ccgggactgt ataatatttt ttccttttta aaaaggaaaa
    17101 acaccactaa aaattcccca atcagctctc ttttttcttc ctctatctcc atttcctcag
    17161 ttaaagagct tgctctctct tttagagagg gggaaaaata ataagaataa cgtattccct
    17221 tcatagaccg attaatagaa ggaaaagacg gaggaagagg ggttacctct tcaattttta
    17281 gctcgggaaa tacttctcca tttaaagtat ttaataactc ctcgggaaat aattcccgag
    17341 aaagagaaac atcccagtat tccgctctgc tctccactcc taaaggtagc gccggaccaa
    17401 aggaaagaag aggacgagga ttaaaaccct gcgaatagac aaccggaagt ccagcccggc
    17461 gaaaagctct gctccatagg cgactcaaat ccagctgaga aataaagtgg aaaggagcca
    17521 ttttttgatg tttcactcga taacgaaatc tgtccatatc agcaaactcc acaattatag
    17581 caattctccc cttcccggca gggaggggtt attttttctt cttgagacaa ttggtattct
    17641 ttccacaaaa aatctttatt tactcctgaa gaaagatgtt cccagggaaa aatttcttct
    17701 ctctctcgtt ctcgatgggt ataaaaattt aagttcaatc cctcctcttc aaatgcttcc
    17761 tgccaccgct ggagagaaaa aaactctctc cagttattca ttactcctcc ttttctatat
    17821 actcgctcaa taactgaaga aattcgccgg tctcctcgac ttaacgctcc ctctatgaga
    17881 ttcacctcaa aatctgacca attgtaacga acccggtggc ccagaggacg aaggagagaa
    17941 tgaagaatag ccattttctc ttctaaagat tctatttcct cctcttttgc ccattgaaaa
    18001 ggagtgtgag atttagggat aaaagcggaa ataccaagat gcactcctct tttctttccc
    18061 cggctcaaaa tctcttttac taagtttgca atacctctta tatcctcttc tctttcccca
    18121 ggaagaccaa tcataaagta gagctttact tctctccagc ctgaggaaaa agtttttccc
    18181 accgtttgaa aaatatcctc atcggtcagg cctttattaa tcactcgccg caatctttca
    18241 ctacccgctt caggagcaaa agttaaccca ctacgacgca cctcctgcaa agactgagct
    18301 atccccacag aaaaagcatc catccgcagc gagggaagag aaacatttac cttagattct
    18361 tttaatctaa aagacaattc tctcaccaga tattcaatag ctgaataatc agtgctactt
    18421 agagaaagga gagaaacttc ttcatagccg gtgcttttta ccagcttttc ggccaattct
    18481 atcacttttt ccggggaacg ttcccggtga ggacgataaa tcattcctgc ctgacagaaa
    18541 cgacaacctc gggtgcaacc ccgggatatt tctaaaggga ttcggtcatg aacaatatta
    18601 aggtaaggaa ctatgatttt ttgagggtaa ggagcactat caaggtcttt tatccacctt
    18661 tttctcacca ccccgggaga aatacttttt atttttccat caggatgata agaaaccgaa
    18721 taaaagcggg gaacataaca gccctctatt ttagatagtt ctgataaaac gttatctcgc
    18781 ttttcacttt tcatagcttt caatacttcc actatttcta ctatcgcttc ctctccctct
    18841 cccagcaaaa agacatcaaa aaaggaagac accggttcag gattaaaagc acaaggtcct
    18901 cctcccaaaa ttaaaggaaa ttcctctcct cgttcttcac tccacagagg tatacccgcc
    18961 aaatccagaa gatataagac attagtataa ttcagctcat gttgcaagct catccccact
    19021 acatcaaagc tacgaagagg aattttactt tctaaagagt agagaggaat atttttttct
    19081 ttcatcattt ttcccatatc cggccaggga gcaaaaactc tttctgctct cactccagga
    19141 agagaattga gaatatggta aagaattttc aaccccaggt gagacattcc tatttcataa
    19201 aggtcgggaa aagctaaagc cacagatatc tcaacatctt cctccttttt catactcata
    19261 ttccattctc ttcctatata gcgagccggc ttactaatag agagcatctc ctgccgggat
    19321 aaaatttttt ctaaatccac aacttccaac tttccttacc tccactttta ctgatattat
    19381 aaaccaaacc agctgaagcc aaatttgcta ccaacgaact ccctccataa cttataaaag
    19441 gtagagcaat cccggtaacc ggcatgactc ctaaattcat acctatgttc actgcccact
    19501 ggaaaagtaa acttattccc agaagagaag ctaaaatctt tccttctaag tcaggagagc
    19561 ggagagctat cactaaaaaa aggtaggata gagctcccaa taaaaatata attaccattc
    19621 ctcctaaaaa tcccagttgt tcacaccagg aagcaaaaat aaaatcagta tgccgagcag
    19681 gtaaaaaata cagctgactc tgcgtaccgg aaagccagcc ctgtccggta aatcctcctg
    19741 ccccaatggc aattttagat tgcaaaatat ggtatccgct acctaaaggg tctttccccg
    19801 gatcaatgaa agagagtaat cgagcttttt gataatcttc caaaaaaaac caggaaagag
    19861 gcaaaagtcc cactcctatt cctcctatta ctgcccattt tttagcactg aagccacaaa
    19921 caaaaagccc tcccaaccag ataaccagta aaaccagaga agaccccagg tctggctctt
    19981 caaaaataag taccagagga ataaaagaaa gtatcccgct tttaagcaga agaggccaga
    20041 aatcaaagtt ctccctctgc tcagaaaaat aatgagccag aaataaagcc agggcaattt
    20101 ttgctccttc cgaaggttgt accgactgag aaaaaagcca ccgacgagaa cctgtttcat
    20161 ccccttttcc aaaaactaaa actgccccca gggataaaac catcaaaaga taccacagcc
    20221 accctctctt tacccattga tggtaatcta tttggctaaa aaagagaaaa actaaccagc
    20281 caacaaaatt ccagataatt tgtttactga ataagcttgc tccccattct tctcctcgca
    20341 agggatcaac actatatact gctaataccc caataagaga aagagcaaca aggattaaaa
    20401 ttagagcttt atcttccact ttttcctcct cgattttctt cccaccattc aatcaatttt
    20461 ttagccgttt gtgctgcctc gccacttcca tcccctccat gttctattaa tactaaaaaa
    20521 actacctgag gatgagaaga agggaaaaat cctgcaaacc aggaatggtc ttctccttga
    20581 ggattctggg ctgtcccggt tttggccgcc agctcaaccg gtaaaccccg gcaaacctgg
    20641 ccagttcccc tatttactac tttttccata ccccggataa gagtctccca ggtactattt
    20701 tttaattcta ttctatcctg taaaactgga gagacctttc taatcacttc tcctttacta
    20761 tccagcacct ctttgagtac atggggttgg taaacttctc ctcgagtggc aatgctactc
    20821 accactcgat acatctcaag aggtgtaacc agaagatacc cctgtccgat agatagatta
    20881 atagtatcac caggatacca catctcttta agctgccttt tcttccattc tggagtaggt
    20941 aaaaaagcag ttttctctcc tggcaggtct atacccgaag gtcggcccac tcctaacctg
    21001 ccagaccagt ttataattct atccgctccc agctctaaac ccagagtata aaaagtaacg
    21061 ttacaagatt gagcgatagc ttcttctatg ctaactgccc cgtggcctcc tcttctccag
    21121 caaggataac gccacccatt ataatccaga aaaccaggac agaaaatctt ccgattccca
    21181 tcggtcaatc cttcttctaa agctccagta gctatcacta atttaaaaag agagccggga
    21241 gggtagagag cttgaagaga gcgattggta agaggtttat cctggttttg agatagggta
    21301 ttccactcct cagcagtcat tccccaactt aagagttgag ggtcaaaacc aggtttactc
    21361 accagcgcca aaagctcccc ggtatacggg tctcctacta taattactcc atttctatta
    21421 cccagcaact cataactata ttcctgaagt tctccatcta aagtcaacgt taccgaattt
    21481 ttaaagtaag gagagcgata atctaaaact cttatcactc ttcccaaagc atctacttct
    21541 attctccgat accccttttc tcccctaaga agagactcat actctcgttc cactcctact
    21601 tttcctattc tatcttccat ctcataacct tcagaataca gtgattctaa ttcttcggcg
    21661 tttatcttcc ctacataacc taccacatgg gcaaatggct ctccacgggg ataatgacga
    21721 accggataag tttccaccct cactcctggt aagtcatttt gggcctcttc tattttaatc
    21781 gcctcttcca ggctcaaagg gtcgattaat attatctctc ctacccctct ttctggaaaa
    21841 ggaggagaaa gttctctccc taaaatcctt tccactcttt tttctatttc ttctacctcc
    21901 attcctcctt tttcataaat taagcggaaa cgaggaatgt cttcagccaa tacttgacta
    21961 ttacggtcat aaatactgcc ccggatagga gaaagagaga aaactctcac tcggttttgt
    22021 tctgatttct gtttgtacca gtctccctgt aaaatactga ggtaccagaa tcgaaaagat
    22081 agaatcagga gtataattaa aaataaccga gaaagataag taattctctg gtttacttgc
    22141 tcctgcattt cgtcattcca catacttgag accccgattt atttctttcc tccgccacca
    22201 ataaaacaaa aaaatattaa gaggaatact caatagcttg gctcctaaaa gaggaaaaaa
    22261 aggcaaagtt actccctcaa tagcagctac tatctctacc agaattaact ccacgagagg
    22321 aaaacagaag aaaatggcta aaagtgaaat agaggtggta gaaaaaaatt tggcccagca
    22381 ataagtgaat aaagccaaac ttgataaaat aaaaagataa gctccccaag gggaaaaaaa
    22441 caaaaggtcc aaagttattc ccagaatcac aataaggaga acatctaaat tatcttctct
    22501 ctcctcccag aacaaagaaa ccattagaaa agaaaaaatc cataaatgac gaagggggaa
    22561 ccacagatgc actagaaaag aagaacttat cccttcccac aaaagaggaa gaaaaaaaaa
    22621 gataccgatt ttctcaatca ttctaacttc tcaggaagcg ctttaatcac aaaaacctta
    22681 tctaaaagag aaaagtcaca gctgggctct acaattacat ttttgaaaag actaccatcg
    22741 tcttcttcta tctgagagac tcttcctaca ataattcctc gaggagtatt ttcttcccca
    22801 cctgaggtta taactatatc tccttctcta aggtcggaag tccgataaat atatttcata
    22861 aggcacaaac cttccccgtt tccagtaacc actcctaaat ctcgggtcct ttgaaccaga
    22921 gcccccactg ataaaaaagg acttaaaaga agtcttatta ctgcatgatg aggataaact
    22981 tcctctattt tacccaccat accctgataa gtaactacta ttaacccctc aactatttct
    23041 tctttctccc cctggtcaat aatagcttta ccccaccaat cataagggtc tctccctatc
    23101 acctgagctg atattacctc aaaaggcagt tcttcttcca tagccccagc ttctattttc
    23161 atttttaatg aaaccacttt ttcctgtaac aaaatattct ctgctctaag tttatctatt
    23221 tcttctttaa gtttttgatt ttcctctccc agagtcctgg agtcagaaaa aaaccttccc
    23281 catccctgaa aaaggtcttt taccctctcc cccgctcgat gaagaggaag aaaaaaagaa
    23341 cgaatattct ctatctggtt atcttcagtt cgaactgccc agattaaaat ctgagaagat
    23401 accactatta atactattag tatcaaaagt ttctggcttc tgtttccgtt catcatcact
    23461 cagcaaaaaa gactttttcc atagaagaag aaagataaat tttatcttct tctatggtta
    23521 aaatttttac ctcggggaat ttcttttcca ctacagccaa agcttctttt cctcctgcca
    23581 taatagcggt agaaagaata tcggcaaaga cagcttcttc gctaatcacc gtcacagcct
    23641 gaaaatctct tcctggataa ccggtgaaag gatttaagat atgatgataa cgcactcccc
    23701 cctcctcaaa aaaccgataa taatctccag aggtagatac tgcttcattc ttgattacca
    23761 cataacctaa aagtttttcc tgacgaggat gacggatccc taccttagtt tccttcccca
    23821 gaacgaaaat ctctcctcca gcattaatta gcgcctgagg aactttttcc tttcgcagga
    23881 gagaaactat ctcatctatc aagtagccct tggctatgcc acccagatct atctggccct
    23941 ctccttcaat taatacttct ctattctctc tattcaaata taatttactg ctatttattt
    24001 ctcctactat cttcttaatt tctctttcct cagggagaga aggttgttca gaaaaacccc
    24061 acaaatccat taaaggagca attaaaacat tgaaaaaccc ctgagtttct tcttcccctt
    24121 tttgtgcttt ttctaacacc cgaaaagtat cttcatctac tgaaagagga agagaagaat
    24181 gatttatttt aaaaattata ctctgaggag aaaaacgatt ccagagttgg tccagatgat
    24241 taactaaaga agaaattgga gaaaaaagag aaacattatc ccggggaagt tctacttcta
    24301 tccaggtatc aaaaccaaaa aaagttttct tctcctcttt atcccggaag aaaaaacccc
    24361 agaacaaaaa agcgaaaagc agaaaaacta ataacgttct tttatgtttc ataaagtatc
    24421 tatccatcta ccaaagaatt ataacagaag aggactacaa gggggtatct ttactataaa
    24481 tactccataa aagcatggct acaatccctt ccgagtagcg accacaatat tgaagagaaa
    24541 acatttctta aagttcacaa ttgacttctg cgaggttaca ataaaaggag cgataattac
    24601 cataggctcc aaagctaatt ataaaaaggt agagaggaac cacaaaaacg gcaatgattt
    24661 cctgaaagcc ccgaagtgtc tatttgatac cccttcctta tcactacctc ctttccacag
    24721 gcaggacaaa aactaatatt tccttttcct cctcctgtgt ttcccaggta aacgaaagga
    24781 agtttttccc gagcaaaatg gtaaacctct tctagaaaag atatcgaagt aggagggtta
    24841 tcaagcttat aagccgggaa atagcgagaa aaatggagag gagtatcggg atttaaatca
    24901 taaatccagt ttactaactt ttcaatcagt tcccagcggt catttaaacc cgggatgatt
    24961 aaattagtta cctccacatg aaggccttct cgaaccgcaa tctcaatagt tctcaaaaca
    25021 ggggaaagag ttcccccaca gtagtggcga taaaagtcat catccataga cttcaggtct
    25081 acattcatcg catctatcca gggtaataac tcccggaggg gctcttcttc tacatagcca
    25141 ttggtaacca gcacattttt aagcccttgc tttcttacca gttgagcggt atcgtagacg
    25201 aactcccacc agatgaaagg ttcattgtaa gtataagata aacctataga atcattttct
    25261 cgagccagca aagctaaatc ttccggattt atttcccgta aagggaaatc tgccggacta
    25321 acttgagaga ttgaccagtt ctggcaaaaa gaacaatgaa gattacaacc tacactccct
    25381 actgagagaa tctctctccc ggggtaaaaa tgatagagag gttttttttc tacagggtct
    25441 aaagctaaag aggaaatttt tccgtaagtt tgagcatata atatcccctc ttcgtttctc
    25501 ctcacccggc aagtaccact tctacctgca gcaattatgc accggtggga acagaggaaa
    25561 cattgtactc ttccattttc taaactagaa aagaagcgag ctattttcaa tcttcttcct
    25621 catatctatc cacagtaaag cagtaaatgg taaatttttc ctggggagaa atacccgctt
    25681 tagccagagc aatacgaatt tgctcctcta cagtatctac tccctcaata ttaggaagca
    25741 acagtccctt tctccatcct cgctctacta taactccata cttttcaggg tccaattcct
    25801 ctaaactgtt cactctctca ggataagata aaatatctac cgatatcctc aagagaggta
    25861 gctcctcttc ttgaagagga ggaaagcgag gatcttgagt agcagcacta actgcgttat
    25921 ggattatttc ctgggctata ttttcatact gaggaatgta agtaccaata caccctcgca
    25981 aatttcctct cttttttaaa gaaacaaaaa ctcctcgtct ctcatttaaa agatatggag
    26041 ggagtccaga aggaagagga aggtatctcc cttcctccaa ataatactct atagatttac
    26101 gagctaaaga agtaataatt ttccctaatt cacttttctc ttccattttc ttcactcttt
    26161 ctctgattaa gataacgtct ccctattacc actgcttgcc aatcatcaaa agcctcatcg
    26221 ggatagagaa aagaaagagg gacaattttt ctccatcctc gagggggatg agcctgaaaa
    26281 tacaacttcc gggcttcttc agagctattg tgctcatcca ccaaagtaaa cgacaagttc
    26341 attttctcta agattttcat tattttttct ctaccggtac tatttcccac caccacttga
    26401 aaaggtctat acttttctct aagaatatta atttctctct ctatctcccc ctctggcaat
    26461 atatcatgga acaacacctc cccttgcaag gagagaattg ccacccctac tttttctctt
    26521 cccgggtcga tagagaaaat tgttttttca gattggtgat caccggtaaa atccatatca
    26581 acaacaccct cgtgagattt ataaagggaa ctttcacccc ctccaccggg taatcctctc
    26641 ttccccagta gttttttgtg tttttctttt aaatcttacg tcgcaaacgc tgaaaaaagc
    26701 tttccccagt gtaactactt taatttttta gctctcatac ttttagttgg tatcacaaaa
    26761 gttctttatt ctttctcctc ctttgagagt atgagagagt atcaccccct tatactttgg
    26821 attcctgata aaagcattca ggaatgacga caaaaaggga taataaaaga cttaccccac
    26881 tgaggaagga gaaacatctt cctcagtaac ttttaatctt tttggggaat ttatccccct
    26941 tatagtcttc accctcaccc taaccctctc ccgtcaaggg agagggaaag aaaatcgaga
    27001 tttgctttta tcttttgttt ttttgggggt tataaggggg ctctgccccc ttatagttct
    27061 acattttctc tcgagctaaa taatgggtat gcaacaagtg atgggctttt tccccattgg
    27121 gctcacccag gaactcctcg tatagttgtt ttatggccgg gttttcatga gatttccgca
    27181 gaggaagatt tttatctacc tggtagatag cttcaattct cttcattcta atctctaaat
    27241 tggtaggtat aggttgccct cctccaccga tacatcctcc cggacaagcc ataatctcaa
    27301 taaaatgata tttagagttt ccttccttaa cccggtctaa aattttccgg gcattaccca
    27361 agctatgagc tacagctacg tttacctcta aaccatcaac attaaccgta gcttccttca
    27421 ctccctccat tcctcgcaca tcatagaaat caatactcgg aagagtttta ccactaacaa
    27481 tttcataaac ggagcgaagc gctgcttcca taactcctcc tgttgctcca aaaactgctg
    27541 ccgccccggt agaaataccc agggggtcat cgaactcttc ctcaggcatc tctcttaaat
    27601 cgataccctt ttctttgagc atccgggctg cttctcgagt ggtaagagaa taatccacat
    27661 cctgacagcc actggatttc atctcctccc gctgacactc aaatttctta gccgtacaag
    27721 gcataatgga gactactacc atatcctgag ggtcaattcc taatttctga gcatagtacg
    27781 ttttagccac cgctccaaac atttgctggg gagatttaca ggtagaaaca ttttccatta
    27841 gctcaggata aaaatgctcc ataaacttta tccagcccgg gctgcaagaa gttatgagag
    27901 gtagttttcc tcccttttgc aaacgattga taaattctgt cccttcttca ataatggtaa
    27961 ggtcggcagc aaaattggta tcaaatatcc ggtcaaatcc caacctccgg aatgccgcca
    28021 ccattttacc agtggttact gttccggggg gtaaaccaaa aggctcccca atactcactc
    28081 ttatagccgg agcagtttgc aaaaccacaa atttttccgg gtcttctaag gcttcccaca
    28141 ccatttctac atgagaaact tcagaaagtg cccctgtggg acaggacaga atacactgcc
    28201 cacaattaac acacacactc tctcctaaag ggagacctaa agaagggctg actaaactat
    28261 caaaaccccg gttagcaaaa tcaatggcaa aaacggtttg tatttcctgg caagccctga
    28321 tacaccgacc acacaggata cagcggttag catctctttt aactgaaggg ctgctccggt
    28381 ctacttcgta ggtttttttc tcccccggaa agcgaatctc tcgcacacca aattgatagg
    28441 acaggtcttg cagctcgcaa gaaccattgc ggtcacataa ttgacaatcc agaggatggt
    28501 tagaaagcaa aagttctaga ttagttttac gagcttctct cactttttta gaagaagtcc
    28561 tgattttcat gccctctcgt accgcagtta cacaggaagc cacaagatta ggttgtccct
    28621 ccacttctac cacacagatc cggcatcctc cccagggagt caacccttca agataacaaa
    28681 gagtaggaat ctttatccct tccatattgc aagcttctaa aacgctaact ccttctggca
    28741 ccttcacttc tcttccatct aaataaatag taactaactt tttttgaccc atttcttctt
    28801 tcatttcctc tgctacttta gccataggcc cttcaactcc ttactccaat caaattttta
    28861 gttttagttt tcatagaaca agtatccaca ggacatctct tctcttctat gtgagctcta
    28921 aattcctctt caaaaaagct taaagaacgt aaaagaggat taggagcagc ttgtcctaaa
    28981 ccacaaaaag aggtgctttt tatatgctct cccatatagc gaagattttc aaggtctgag
    29041 aggtctcccc taccttcact tattttattt agaatctcca ctatcctttt agttcctact
    29101 cgacaaggag tacatttccc gcaagattca tggagaaaaa actccataaa gtttctaacc
    29161 acatccacaa tacagtgggt atcatctatc accgttagtg ctcccgaccc taaagctgag
    29221 tcatagcggg aaagagtgtc aaaagccatc gggacatcta aaaggtcagg agtcatagtg
    29281 ccccctgaag gaccaccagt ctgaactaat ttgagaccct taccttctcg gattcctcct
    29341 cctacttcga aaatcagctc tctcaaggta atacccatgg gaacttcgat taaaccccaa
    29401 ttgtttacat taccggtcaa actaaaaact ttagttcccg gacaggtagg agtcccaata
    29461 gaccggaacc aatctgcccc tcgcaaaatt ataggaggaa tattggccag agtctccaca
    29521 ttgttaatca ctgtgggctt cccccataat cccccctgag taggataagg aggcttattt
    29581 cgtggtattc ctctttttcc ctcaatagac tctaaaagag cagtttcttc tccacagatg
    29641 taagctcctg ctcctttacg aatactcact tcaaagttaa aaccactgtt aagtatgttt
    29701 tctcctagaa gaccatattc ccgggcttgg tctatagcta tcttcaaatg attaacactc
    29761 tggggatatt ctccccggac ataaatataa cctcgctgcg atcctgttgc ataaccagcg
    29821 atagccatag cttcaattat ggaatgaggg tcgccttcta aaatgagccg gtctttaaaa
    29881 gtcccaggct ctccctcgtc agcgttacac actatatatt tttcctctcc ttctgccttt
    29941 ctggtaagct cccactttaa accggtagga aaacctgctc ctcctcgacc cacaagccct
    30001 gattttttta cttctgctat tacctcatcg ggagtcattt cacttattgc tcgggaaaga
    30061 gctaaatacc catcccgagc gatatattct tcaatactgg tagggtcaat ttggccactg
    30121 ttacgcaaca ctaccctctt ttccttagat aggggaaaat aaggcaaaaa agcacctgct
    30181 tctccccttc cagaaggaga aaaaatcaaa tcttttactg gccttccttt ccaaaaatgt
    30241 tcttccacaa tcctttttac atcactcaac tccactggag cataaataat attatccggg
    30301 taaaccacca caaaaggaga aggaagggcc gctccaaggc taccagtttc cagaacttta
    30361 atttctttat ctaaacctaa tctctcgact tctctttcca gcctctcttt aatttgagct
    30421 gctccccgaa ccagagaatt actggtcatt tccactaaaa catggctacg ataaaacata
    30481 ggcgtcctcc taccgataaa gagctaaaac ttctcggatt ctatcttccg agagattgcc
    30541 ataagttaca tcattaatca tcatagcggg agcaacgcca cagactccca aacagctagc
    30601 cgtctccaga gtaaatttac catcccggga agtttcccct tctctaatac ctaattcctt
    30661 ttgcagagtt ttcaaaacat ccaaggagcc attgacatga caaggagcac tgcggcaaac
    30721 tctaattaca tattcaccca ctggctttgt ctccaaaaaa gaataaaaag tggccactcc
    30781 ccataccacg ctggatggaa catttaattt ccgggataca tatcctaaaa catcaccagg
    30841 gagatatcgc catttttcct gtatctcttg cagaatagaa agaagaggag ttttctcctc
    30901 ttgatattta tcaataattt catctatctc ctcataaacc gctagctcag tcattgaacc
    30961 tcctcctcag ccatacctat ctcctgatat tttttcaata ttttttccag atcacatcgt
    31021 aaacaccgtt ctgcctctcg acgagcatct tcttcctcta agcagagctt cacttctgaa
    31081 aaagaacaca ctcgctctct taccggtagc tccggtggtt taactcgagg aatttcttgt
    31141 agaactggtg gttcttctaa aactttaggc cgctctggtt ggattataac ttcttcttcc
    31201 tcgggcttca aatcccgcag atactggtga atgttaaaag ctgctctttt acctagagcc
    31261 atagcatcaa ctacggtagc ttctttacct atagcgtctc ccccggcaaa aactccggga
    31321 atagaggtag aaagagaata tcggtctacc agaatattac ctcgatggtc taaggttaac
    31381 atctctccta agttctccac ttccggaatt tgacctactg cttccaccac catatcgcac
    31441 tcccataaag cttctgctcc ttctatagga acaggtctcc ttcttccact tcggtcaaat
    31501 tctcccagct tcatcctttg caatctaata ccttttactt taccattttc tcctaccact
    31561 tcaagtggag ccatcaaaaa gtggaaaata actccttctt tttctgcatc ttctatttct
    31621 tcaggaatag caggcatttc tcctctagta cgtcggtaaa ccacattcac ttccgctcct
    31681 aaacggagaa gagtccgagc agcatccata gctgcatttc ctcctcctac gactactgct
    31741 ttacgaggaa cctcaatctg cttccctaaa ttaaaatctc gcaaaaagtc gagagcactg
    31801 accactccag agagattttc gccaggaata cctaaaggag cactcttcca ggctcctata
    31861 cccaggaaaa tagcatcaaa tttttctcga acttccttcc actctatttc tttacccact
    31921 gctttattga gctctatttt tactcccata gctttaatct gattgatatc atattccaga
    31981 gcttctcgag gtaaacgata aggaggtatc cctgcagcta acattcctcc cgctacagga
    32041 agagcttcaa aaatggttac cgaataaccc agtcgagcca gataataagc acaagtaagt
    32101 cccgccggac cagaacctac aatggctact gattcatctc tttttctatc cataaccact
    32161 ggtaaagcta tccgattttg tcgggcataa tcagccacaa aacgcttcaa attatcaata
    32221 gctaccggct catccatatc tcctcgccgg catttactct cacagggtcg atgacaaacc
    32281 cgaccacaaa ctgaaggaag ggggttatct tccctgataa tctgtaaagc agccaaatag
    32341 tcgccctccc taatgcaaga aacataaccg ggaatattta tacccagagg gcaggcatgc
    32401 tggcaaggag ctacaaaaag tggccgacat accactgccg gacacttttt ctctcgaatg
    32461 tgagcctcat attcatctcg aaaataacga atagtgctta aaaccggatt gggagcagtc
    32521 tgccccaaac cacacaaagc agtggtctta atctggttag ctaaatcttc taagcgctct
    32581 atatctcctt cttttccctc tccctgagta attctctgta aaatttctaa catcctttta
    32641 gttcccaccc ggcagggagt gcatttccca caagattcgt cttgaacaaa ctctaagaaa
    32701 aagcgagcca gatccaccat acaggtatct tcatccataa ctataagacc acccgagccc
    32761 ataatggctc ccagctcctt caaagagtcg taatcaattt taatattgag atgctcaact
    32821 gggatacatc cccccgaggg tcctccaatt tgcactgcct tcagtttttt gcctcctcga
    32881 atacctcctc ctaagtcgta gataacctct cccaggctga tacccatagg tacttccact
    32941 aaaccggtgt tatttacatc tccagccagg gcaaaaactt tagttccttt actattctct
    33001 gctccaatag agcggaacca gtctgctcct cgaaatatga tagggggaat attggcgtaa
    33061 gtttccacat tgttaatcac tgtgggtttt ccccacaatc cttcctgagc gggaaaagga
    33121 ggtttgggac gaggcatgcc tctttttcct tctaccgatg caattaaagc agtttcttct
    33181 ccacagacaa aagctcccgc tccgatacga atctcgacat caaagttaaa accactgtta
    33241 agtatgtttt ctcccagaag accatattcc cgggcttggt ctatagcata ccccagccgt
    33301 tctaccgcta aaggatattc tgctcgaacg taaacataac ctttttctgc tcccactgca
    33361 taaccggcga tagtcatagc ttcaatcaca gaatgagggt cgccttctaa aatgcttcta
    33421 tccataaaag ctcccgggtc tccttcatca gcattacaga tgataaattt aggatgacca
    33481 ggagctttcc gggtgagctc ccactttaaa ccggtaggaa aacctgctcc tcctcgaccc
    33541 cgaagccctg atttttttac ttctgctatt acttcatcgg gagtcatttc acttattgct
    33601 cgggaaagag ctaaataccc atcccgggcg atatattctt caatatttag aggattaatg
    33661 aaaccacaat ttcgcagagc aatttttact tgcttatcaa aaaaatcaat ttcccgcaaa
    33721 aacctttttc tttcctcctg aggaggttga tacaataatc tctctaccac tcttcctttg
    33781 agaagatgtt cttcaactat ctcttgcgca tcttcaggag ttagtttctg ataaaaaact
    33841 ccctccgggt agatgatagc taatggcccc agattacaag gacccacaca accagtctca
    33901 atcagcctta cctcattatc caaggcgttt ttttgaagct cccttattag agcttctttt
    33961 attttcacag ctccggaaga aatacaagct gcaccagcac atagtagtat ctgacacctt
    34021 tcctgttcgg ccatccactt tacctcctcg ctctactttc agcatgcact acccaatctc
    34081 ccacaatctg gccattaacc aggtgagaag ctattatttg tcgagctttg tcgggagtga
    34141 ggtcaccata taaaactcgt tcttctcctt tttccacact cactaaaggt tctcggtcac
    34201 agagcccaat gcaaccagcc tgagtaacca taacgttagt aaggcctcgt ttagcaattt
    34261 catctaaaat ggctgacatt acctctcttg ctcctgccgc tataccacag gttcccatat
    34321 gcactaccac tttataatca gcctttcctt ctcgtaatag agttcttttc tgtgcttctt
    34381 ctttaatccg ttttaaatct tctatactat ttactttcat tctccattcc cccttttaaa
    34441 atttcttctt gatattcaat ccactcccgg atggcaggca aaagtacact ccggttttct
    34501 ccggttaaat ctaattcctt ctccatcttt ttactattaa acaaccactg gcaatcattt
    34561 attcggtgga taaattccaa ttccacttct ggatgagaag ccataattac aaaaagagta
    34621 tcagccaatt ttcccagagg gggcaaatca agactatctc tttgaaaagt tgctcgcaca
    34681 gtagtgccct tgcctttttc cgataatatc tctaaatcac cctgacactg cttagctaac
    34741 tgatccagaa gagggatacc cattcccact ttcttaccct gtccggagaa aaaagggtct
    34801 ctcaccattt ttaaagtttc tgcatccata ccttccccgt tatcttcaat aaccaactcc
    34861 aggcgatttt cccgggtatc ctccaggata gaaattctta cttttgtggc tccagcccgg
    34921 agggaattct ccaccacatc taaaatgtgt aaagaaagtt ccttcataaa tcaggaatag
    34981 ctcttaacta tctccgtaat ttgagccggg tttaccctct tataaatatc attatcaatc
    35041 ttaaccaccg gtgctaggcc acaagctcca gcacaacgca ttacttctag agaatacaat
    35101 ctatcttcag tcacttctcc tactccaatt tctaaatcct gtaccaggcg attaagaatt
    35161 tcttgccctc ccctcacata gcaagctgtt cctaagcaaa cctggatgag atgttttcct
    35221 cgaggtttta gagagaaaaa gtgataaaaa gtaaccaccc cataaatctc gctcaaggga
    35281 atgtccagag tgtgagaaac tttttttaat accgggggcg gtaaataacc aattaagttc
    35341 tgaacagcgt gcaaagcttg aattaaacta ccgggttttc ctttaaattt ctgcaaaatt
    35401 acctccaggg cttcttcctg ttctggagaa attgcttctt tctcagctaa gaggtcagcc
    35461 ataatttttt cctccctata attgtttaaa tgtattataa ttcattacta aaagtagttc
    35521 aattggatta cttttccgga cagaaacttt cctaaaatca gcccggcaaa gagaataaat
    35581 ggccagcttt gagctcttta aaaaatggag gtctttgtgc tataatccac ccgattttaa
    35641 aaaggaagga cttcattgtt agaaacattg attattgcta taggattagg tttagatgct
    35701 ttttctttag ccacggcttt cgggatgtgt cagaagacct gcccccaaga tgccaaatta
    35761 cgtctttctt tttcttttgg tgcctttcag tttttcatgc ccttggtagg ttttggaata
    35821 ggttcacaag tagcacaact tatcagttcc tttgaccact gggtagtttt aggaacatta
    35881 tctttagtag gagggaagat gatttatgaa gggtggaaaa gagacgatga tacacccttc
    35941 gttgatttca gccagggctg gcctctcctt tttgcttctt tagctaccag tatcgatgcc
    36001 ctgatggtag gattttcctt tgctcttatg gataaaaata tctggttttc agctctgatt
    36061 ataggaatag tagcattgct tatgacttat ttaggagttt ctctgggaca caaaataaga
    36121 ggaatttttt cctctcccga ggtagtggga ggaatagcta tagtacttgt ggggttaaag
    36181 attttcttag aagacttata gaaagaagga aaaggaatgg caaaaggatg tatttttgga
    36241 ccgatattat cacggagatt aggtagctct ctgggaatta atctccttcc ttataagact
    36301 tgcagcttaa actgtatata ctgtgaatgc ggagcaacca ccaatttaac catagagaga
    36361 aaagaatata ttcccaccca agaggttatg gaaagtctca aaaaatatgc tgcctcttcc
    36421 cgatttcgca gtgcccctcc tttttgtatc acttttgctg gttggggaga gcccaccctt
    36481 cattcttctc tgggagaaat tgcccatttt attaaacagc tcttccccga acaaaaatta
    36541 gttctaatca ccaacggaac tctttttggt ttccacgagg agctcttaga agaagtaagg
    36601 gaaatggata tcataatccc ttccctggac gccgcttcct ctcaggcttt ccagattatt
    36661 gaccggcctc atcctcagat tactctcgat tcctacgtag agggtttagt taaattgcgc
    36721 aaacatttcc ggggagaaat atggttggaa atttttataa tagaggggat aaatgactca
    36781 gaaaaagaat tgaattttct taaagagaaa attgctctta tttcccctca tcgggtacaa
    36841 attaacgcct tggaccgccg acctgcccag gaagggataa aatcccctcc tctttatcgg
    36901 ctggaagaaa tagcttcctt tttaggaggg gaagtaattc tacccttacc gaaacagtct
    36961 aaa
//
LOCUS       DAOSVE010000006        34292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_889_length_34292_cov_69.265006, whole genome shotgun sequence.
ACCESSION   DAOSVE010000006 DAOSVE010000000
VERSION     DAOSVE010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 34292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34292
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_889_length_34292_cov_69.265006"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2428)
                     /locus_tag="PK016_01080"
     CDS             complement(<1..2428)
                     /locus_tag="PK016_01080"
                     /inference="COORDINATES: protein motif:HMM:NF017069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXL01706.1"
                     /translation="MNITYVLPDTSLWGGIKIPLEQASRLHKRGHRVRVIAVKDKPDW
                     FKIDFEFISVPKLTPRVIPQDSELIIGTFYPTVVVAHDAGFGIPIHYCQGYEYFGTDD
                     PRAKNYIDFVYSLYTVKVVNNLFLKKILEEKYHHPTYMIHNAIDLSLFHPENRATFPK
                     KRVLIVGPIEVEWKDIRTALKAAKILKTKLGNAVEIIRVSQFPISQEEQFLGVVDEYY
                     NHLSGEEMAKVYSSCDVLMASSKGKEEGFGLPPIEAMACGTPVVLTDISAYRSYDEVT
                     DYALFVPPQDPETMAQAVIHLLEDKDLYTRLQKRGLEVAKKYNWDIVENEVEAVFSEI
                     KANHWEEFKLKSERMFPGKSDSLTEAMHFQRYRFASQFVKDKVVLDAGCGAGYGSWIM
                     ATEGGAREVIGIDYAREIIEYDLEKYQKDNLTFETMNVLDLAFEDNKFDVVVCFEVFE
                     HIDDPDVLLKELSRVLKPDGMLIVSTPNKTVYSPDKEPGNPFHVREYSYQEFRSILAK
                     YFHNFKFIGQYLSSGKLELRTDNGVLIERALAFIAIVQNPLKRKELYLSEGKSLIYYS
                     YSRPEIQALIPSSAKKILDIGCGAGELGKALKERQDCEVTGIEIVPEVADKAREKLDK
                     VIVGDIEEIIDDLPENYYDVVIFADVLEHLKNPWKILSQIKTRINAQGIVIISVPNVR
                     HWSVIKDLLEGRWDYQEAGILDKTHLRFFTKKTILDMLDKTGFKATNLQGTILQGAEV
                     PPGFLHALKTSGMKLFTLEEEGRIYQYLLVAQPKEVTPKTLSLTSIVILTRNNLEYTK
                     MCLESIRRYTP"
     gene            2667..3398
                     /locus_tag="PK016_01085"
     CDS             2667..3398
                     /locus_tag="PK016_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018977715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HXL01707.1"
                     /translation="MKGLILAGGTGSRLYPLTKVTNKHLLPVGRYPMIYYPIYHLTQI
                     GIQDILVVTGTEHMGKVVQLLGSGRDFGVKFTYKVQDEAGGIAQALGMGREFVGEDNC
                     LVLLGDNIFEDDLTLYFTHFQNQKRGARLLLKEVPDPERFGVPVFKEGKIIAIEEKPT
                     SPKSSFCVAGIYFYDNRVFDVISQLKPSSRGELEITDVNNWYIERGEAEYDILSGFWT
                     DAGTFESLSRANELTRDMTLDFGRE"
     gene            3404..3880
                     /locus_tag="PK016_01090"
     CDS             3404..3880
                     /locus_tag="PK016_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HXL01708.1"
                     /translation="MKFTPGDIKGVIKEELARYEDKRGWLIEVFRQDELLEDLYPLMG
                     YISLTHPGVTRGPHEHRFQWDHFIFLGSSVFRLFLWDNRPSSPTYQNQLILIAEEDHP
                     LRVVIPPGVVHAYQNRGEKPGLVLNFPNQLYRGWGKKEEVDEIRWEERKDSPFVVE"
     gene            3884..4750
                     /gene="rfbD"
                     /locus_tag="PK016_01095"
     CDS             3884..4750
                     /gene="rfbD"
                     /locus_tag="PK016_01095"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HXL01709.1"
                     /translation="MKLLITGSQGQLGREIQKEGEKRGYQVYPFSHEELDITKISAVE
                     TAVQELKPDIIVNCAAYNQVDQAEKERETAWKVNVEGVENLALSSRKHGSFLVHYSTD
                     YVFSGNQSHPYREEDLPSPLNYYGKTKWEGEKALEKVLSPSQFLLLRVSWVFGNNPKV
                     SFPLKLLQWSREKQTLRIVDDQISSPTYAHDAAYFTLELLEKDAQGLFHFANSGYCSR
                     YQWAKFILEKLNWRGQLFPAKSSDFPTSAQRPPFSALDSRKVETVLKRKIPSWEEATD
                     RFLLSLKEGKNE"
     gene            4743..5732
                     /gene="rfbB"
                     /locus_tag="PK016_01100"
     CDS             4743..5732
                     /gene="rfbB"
                     /locus_tag="PK016_01100"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012460055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HXL01710.1"
                     /translation="MNRILISGGAGFIGANLVKLLLEWEPEWKIVVADALTYAGDKKR
                     LEEISDNPRFSFYQVDITQKEKMREIWEREKPQGVIHLAAESHVDRSIKDPSPFILTN
                     VLGTEVLLELSLEFGVSRFLHVSTDEVYGELKDKEGKFTEEHPLAPSSPYSASKAGGE
                     LLVKAYERTFNLPIVIVRPSNNYGPWQYPEKLIPVVIWQALHHQPVPVYGRGENWREW
                     LYVEDCAQGIYAAFLKGREGEIYNLGSGEERQNLDTVKTILSLLGEKEDLIQFVPDRP
                     GHDFRYSLDSQKAERELNWRAKTKFAEGIKKTVDWYIKNIKWVEEKIKEINSL"
     gene            6653..7402
                     /locus_tag="PK016_01105"
     CDS             6653..7402
                     /locus_tag="PK016_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HXL01711.1"
                     /translation="MSGHSKWAQIKHKKAKTDSARGKAFSKLIRLITVAARQGGGNPE
                     NNARLRIAIQKAKEINMPQENIEKAIKKGTGELEGVNYEEITYEGYGPGGVAVMIEVM
                     TDNRNRTTAELRHILSRHGGALGESGSVSWIFERKGFISFDKEKVDEEELMTVAIEAG
                     AEDLKVGESTIDVITSPSDFDQVREAIERAGLSYDLAQITMLPKTMVNVEGKAAQQVL
                     ELIDALEEHDDVQEVYSNFEVADEVLESLNS"
     gene            7403..7894
                     /gene="ruvC"
                     /locus_tag="PK016_01110"
     CDS             7403..7894
                     /gene="ruvC"
                     /locus_tag="PK016_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HXL01712.1"
                     /translation="MSESTCILGVDPGTAITGFGIIESFAQGNSLRVVDCGFIATDKG
                     LPASARLKLIFEDLKTVIEKFAPQEMAVEELFFNKNSKTAISVGEARGIVLLCAALYQ
                     VPVYEYTPLEVKQSVVGYGRATKSQIIYMVKQILKIEKEINPDDVADALAVAICHAFR
                     SGM"
     gene            7900..8496
                     /locus_tag="PK016_01115"
     CDS             7900..8496
                     /locus_tag="PK016_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HXL01713.1"
                     /translation="MIDYLRGKVVEMDEEDWVVETNNFGFRLKVTPFVLPGNIGEERC
                     FLLRIFMKENGEFIFYGFDNEEERSVFDQLREIRGVNHRTAFKILSRVKWEELVKLIL
                     NEEISVLETRTSLSSKTIKRLVLELKPRFLKKGWKIEGGERSGEVVEEVREVLSRLGY
                     RTVEIEGVIDDLWGELGREGADVETWIKEALIKLGGGV"
     gene            8493..9464
                     /gene="ruvB"
                     /locus_tag="PK016_01120"
     CDS             8493..9464
                     /gene="ruvB"
                     /locus_tag="PK016_01120"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HXL01714.1"
                     /translation="MKRESVDDFLIRPQSLADFVGQEKVKSNLQVYIQAARSRGEPLD
                     HVLLFGPPGLGKTTLANIIAHEMKTSLRYLSGPAFTRGGDLASVFTTLSAGEVVFIDE
                     IHRLPPPCEEILYGAMEDFALHLVVGKGPGARSIRIQLPLFTLVGATTRVSLLSSPLR
                     NRFGIVEKLDFYQEEEITKIVERAAQVIGVEIQPRGAEEIAHRSRGTPRIANRLLRRV
                     RDFAQFFGEEIINEEIAKRAFTYLGVDEVGLTDFDRRFLEVLVKYYQGGPVGIENIAS
                     ILGEDPATLEEVYEPFLLKLGFIARTKRGRIITARGRQYLQKVKEVG"
     gene            9457..10023
                     /locus_tag="PK016_01125"
     CDS             9457..10023
                     /locus_tag="PK016_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HXL01715.1"
                     /translation="MADKVLLHTCCAPCATYVNEALEKEGWEVVNIFYNPNIHPFDEY
                     ARRYETLVLYLENEGKVLEAPFPYNPLEYFSLITEPENRCPKCYFLRLRKVAQWGKER
                     GFKFFTTTLTISPYQDLPAIWEVGKQIEEKLDIKFLIRDFREGFRQSVARSSELKLYR
                     QNYCGCIFSKYEGAKRRLEKRLTGLKFS"
     gene            10026..10220
                     /locus_tag="PK016_01130"
     CDS             10026..10220
                     /locus_tag="PK016_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HXL01716.1"
                     /translation="MGLVFIVVGVLLLLLSRIPGVSKLGHLPGDIVYRRGNFVFYFPL
                     MTCLIISVILTLIFTLLFRR"
     gene            10230..11555
                     /locus_tag="PK016_01135"
     CDS             10230..11555
                     /locus_tag="PK016_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HXL01717.1"
                     /translation="MLKKIVLILCLILFVSLNEGWGKELEVTVNLNKAKEAVFSSSGG
                     LKVEIGKRSFSVPPSYPVHFREEGEEIKWEEENLLSSEVYIYPKGKEAVLWGKRPYRG
                     SFIIKASDGGILVINKLPLEDYIKGTMKLELSPSWPVEALKAQIIVSRTYALRNLRRH
                     EEEGFNFCATEHCQRYGGINAEDPVTNRLVEETKGLVLTYQNELASVVFHSESGGFTE
                     SAFDVWGREVPYLIEVSSPWEENAPHSSWEVEFKKEEVENCLNKAGLIQGEILDINLS
                     SSPEGRVEEVEVITSQGLFSFPASRFREALGWEKLPSTFFVMRKVEKEQKVRKETFPS
                     PSSSSASSFDYEEWMDKDWDLDDIINFLKMREEERNRKKGISSPPRIVVEEEVIPQGT
                     TFIFEGKGYGHGVGLSQWGAKGMAEEGFSCQEILNHYFPGCKIERATFK"
     gene            11552..12583
                     /gene="queA"
                     /locus_tag="PK016_01140"
     CDS             11552..12583
                     /gene="queA"
                     /locus_tag="PK016_01140"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012644977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HXL01718.1"
                     /translation="MSGLVSEFDFSLHPELIAQEPFYPRDHCRLMVVDREQKTWEHRF
                     FYEIVEYLEPPDVLVINDTRVLSARFWVYKEETGGRVEILFLRHRGEEWECLLRPSRK
                     VKTGQVLKLEKDPRFSWRVRERNNGIWLIEPLFSSSEEEKLFDSFGEVPTPPYVKNRA
                     INLADYQTVYARIPGSVAAPTAGLHFTPHLLKKLEEKGVKIVSLTLHIGLGTFRPVKE
                     ERIEDHNMHSEHFLLTESAAQTILEAKEKGGKVVAVGTTVVRVLEAVWQQFNTLKGRE
                     GETNLFIYPGFHFQAIDWLITNFHLPRSTLFMLVCALAGTEFMKEAYEEAIKKGYRFF
                     SFGDAMLIK"
     gene            12595..13728
                     /gene="tgt"
                     /locus_tag="PK016_01145"
     CDS             12595..13728
                     /gene="tgt"
                     /locus_tag="PK016_01145"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HXL01719.1"
                     /translation="MGDFELLVSDSRSRARLGLLHTAHGVIETPVFMPVGTQGTVKAI
                     SPDRLEEMGVKIFLCNTYHLHLRPGEDIVKEAGGLHSFISWPHSLLTDSGGFQVFSLT
                     DLVEVDDDGVYFRSHLDGSLHRFTPEVALQIQMDLGADIIVTLDQCIGFPASSHQERL
                     GAKRTLQWAKRSKEFFSSPHQLLFGIIQGGMDEDLRICCAKETVAMNFAGYALGGLSV
                     GEPPPLTYKMIERVEPFLPPDKPRYLMGVGTPESIIEGVRRGIDMFDCVLPTRNARNG
                     CLLTRRGKINIDRQEFSRDFRPVDPECHCYTCLNFSRAYLRHLFKCKEILASELATIH
                     NLYFMVELMENIRKSLREGYFLDFAEEFLAEYESNPFSSFSKK"
     gene            13744..14076
                     /gene="yajC"
                     /locus_tag="PK016_01150"
     CDS             13744..14076
                     /gene="yajC"
                     /locus_tag="PK016_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HXL01720.1"
                     /translation="MMNWFISLAHAANEVPAPATPATPAAPSIIAQLFPLIFLIVIFY
                     FLLIRPQQQRQKKQTELLNSIQKGDKVVTIGGIHGTVTQVDDEAVLLEVDKNTKIRLS
                     KSAIAGKE"
     gene            14111..15298
                     /gene="secD"
                     /locus_tag="PK016_01155"
     CDS             14111..15298
                     /gene="secD"
                     /locus_tag="PK016_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HXL01721.1"
                     /translation="MRRSNLPWRLIISIALVVVSLVVIFPVRQKIRLGLDLQGGSHIL
                     LECVDTPEAPVDSDAVNRVVEIIRNRVDQLGVAEPVIARQGERRVLVQLPGVTDPQRA
                     VEVIGRTALLEFKDEEGNTLLTGAHLKNAQVQYDRFGRPVVSIAFDEEGAKLFAQATT
                     TNVGKPIGIYLDGNLISNPVVQEPIMGGEAQITGRFSLEEAQNLAILLRAGALPVKVD
                     VIENRSVEPSLGQDSIIYGLRAAVVGALLVVAFMIFYYGWLGVLADLALASFTVFFLA
                     LFISLRATLTLPGIAGFIVTVGMAVDANVLIFERIKEEYRTGKTWRASIEAGFNKAFR
                     TILDSNITTLIAAAMMFYLGSGPVRGLGVTLTLGILCSMFTGIWVTRALVDFFAERIR
                     FKA"
     gene            15319..16200
                     /gene="secF"
                     /locus_tag="PK016_01160"
     CDS             15319..16200
                     /gene="secF"
                     /locus_tag="PK016_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HXL01722.1"
                     /translation="MELIKNPNFNFVGNRRIAYAISIVIIAVGLISLLVQGLNYGVDF
                     VGGTLLQFRFNREVEVEEVREALEGLNLAKSTVQRFSPQEVIIRSPKLSQEEQDQVEK
                     TLGEAFGEVTLVRVEDVGPAVGADLRRMAIVAVLVALLGILLYVSIRFEFRPGVTSII
                     ALVHDALVTLGMLSLLQREFSAPVLAAILTILGYSINDSIVVMDRVRENLRSRKKGEG
                     FGELVNRSINETLSRTINTVLTTILPVLALLILGGEMLRDFSLTLLIGLIAGTYSSIF
                     IVSSLWVEWEEKKPLRR"
     gene            16207..17904
                     /gene="recJ"
                     /locus_tag="PK016_01165"
     CDS             16207..17904
                     /gene="recJ"
                     /locus_tag="PK016_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HXL01723.1"
                     /translation="MGKKWQIRKVNEGYLKNLSRSLSMPLSIARVLMNRGVFTLQEAQ
                     DFLEPRLSFLRKLEELPYLKEAVLLLDQVIRKQEKILIFGDYDADGLTAAAILARFLQ
                     LFSPEVEIYIPNRFREGYGLGREAVKEVLKREPHLVVTVDCGIRDREGIATLKSQGVL
                     VLITDHHLPDFDLWPQGVCVVSTWDRDSREMISPLSGVGIALALAQAYGEYRGAKINP
                     VEEYLDLACIGTVSDVVPLLGENRVIVRYGLEKLNRNPCCGLRALSEVSGLAGKTIGT
                     QEVGFILAPRINAAGRVEDSQPALDLLLSDKYEEALELANYLSTLNYRRQKEEEKVLE
                     SIFQDEKNEAYLQDEVVVISGSGWSQGVLGIVASRLSEQLDKPVIVLTEIDGMAMGSG
                     RSITGFSLFSVLERSSSFLVRYGGHEMAAGLRLFADKVEGLRNFLNDSFAEEVKKARS
                     LRGLVVDALVGLQEVNEETLFWLEKLKPFGEKNPPPLFAVLDASLIKSWIWGKNEQHL
                     RLLVKQGREAQEMVVINGKKKAGNLVNSSLVDLAFEVHRDNSGNYPYLKIQDWRIKK"
     gene            17901..18440
                     /locus_tag="PK016_01170"
     CDS             17901..18440
                     /locus_tag="PK016_01170"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005214020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HXL01724.1"
                     /translation="MKEVDLDVDLAQYIRNIPDFPQLGVQFKDITTLLKDGMAFRETI
                     ERLVGIFEDKGVELVVGIEARGFIIGAPLAYSLGCGFVPVRKAGKLPAPTLSKTYDLE
                     YGSATLEIHRDAIEKGQKVIIVDDLLATGGTTKAVCEMVEELGGKIIGIGFVIELESL
                     KGREKLSSYPVYSLIRFNI"
     gene            18455..20683
                     /locus_tag="PK016_01175"
     CDS             18455..20683
                     /locus_tag="PK016_01175"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HXL01725.1"
                     /translation="MKDRFRCKNKKIGEVGFFGWSGVETIESLLDKINLYHPDFNSDL
                     INSAFSFSLEAHRNQTRLSGEPFIIHPLAVAHIVADLRMDENTIAASLLHDVLEDSEV
                     TEEDLKKNFGEEVTNLVKGVTKLNRGFSYVSREEAEVENYRRLFIAMAQDVRVIIIKL
                     ADRLHNMRTLKYQYRDKKEIIARETLEIFAPIAHRLGINKIQTELEDLSLFFLDPGAY
                     CNIQRGIERVKEAQEKQIEEAKKELEVRLEKAGIEAEIQSRFKHIYSIYNKLNRQKIS
                     LEDLPDLIALRIIAREVENCYAALGIVHTLWKPVEGRFKDYIAVPKSNMYQSLHTTVI
                     GPRGAPMEVQIRTWDMHQVAEYGVAAHWRYKEGKTGESDDKFDERMNWLRQILEWQQD
                     LRSTQEFMESLKISLFDDEVYVFTPKGDLKKLPQGSTPVDFAYLIHTEVGNKCVGAKV
                     NRQMVPLSYELKSGDIVEIITSRSSSGPSADWLKIAKTPGARNKIRAFLRKKNQDLNR
                     EKGREALEKELDKYSLTPEEKALVARNLSQFREENQLREEEELYIAIGEGRYSARQVA
                     NKVVPYTIRRRLQKKKKESGAITREEERIVVQGATGLAVKLARCCTPVPGDQIVAFVT
                     KGKGITIHRVDCPNLTRLSQDGERFLEASWGEEEGIKYHTGVVIEALDRPKLLADISN
                     AISNFQVEIKAIRANTIGNEARIYLILEVKDRQELDGVFREVKKIANVRGVRRGGAFA
                     LR"
     gene            20680..21135
                     /gene="dtd"
                     /locus_tag="PK016_01180"
     CDS             20680..21135
                     /gene="dtd"
                     /locus_tag="PK016_01180"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HXL01726.1"
                     /translation="MKAVVQRVKRARVKVEEKIVGEIEKGLLVFLGVHRDDGEKDAQF
                     LINKVPYLRIFEDGEGKMNRNLKEVGGEILLISQFTLYGDCRRGLRPSFDQAAPPEEA
                     EKWYEEVGKGWEKEGIKVERGVFGAFMEVEIHNQGPVTIILDSKERRNK"
     gene            21132..21782
                     /locus_tag="PK016_01185"
     CDS             21132..21782
                     /locus_tag="PK016_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXL01727.1"
                     /translation="MTLKLEHFVLGDLFTNCYLVHDNLEALLIDPAAPSQEIKEEIEA
                     RNLNLQYIINTHGHIDHIGGNVFFKEHFPQARLVIGERDLVYLINPDLNLSRDFSSPF
                     VSPLPDWVVRENNLFLNTSWERLSFLLTPGHTPGSVCLFFPVENWLFSGDTLFAGSVG
                     RTDLPGGSFRDLISSLEEIFNRFPDSAFVFPGHGPFTSLAREKKENFYYLQYLAPD"
     gene            complement(21779..23137)
                     /gene="glmM"
                     /locus_tag="PK016_01190"
     CDS             complement(21779..23137)
                     /gene="glmM"
                     /locus_tag="PK016_01190"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HXL01728.1"
                     /translation="MRRLFGTFGIRRVANEVLTPEMATKLALSFGTFISGEEVVVGRD
                     ARKHSEMLYYAVISGLLSAGCSVTELGVTATPALQWACRQWNQWGAMITASHNPPQWN
                     GIKFMEKTGKGLDREKDEEVEKIYFGEQFTRVSGMKIQSETKKRDIRWDYIEAIIQKV
                     DAQAIKERRFRVALDCANGAPSLITPYLLSELGCQVVSLNSHPDGNFPGRNPEPVPEN
                     LQDLIALIKNSPFAVGFAQDGDGDRLIVIKENGEFVPGDLSVSLVARELAHRETPGPI
                     ATTVATTHILQEIARETNREVIITEVGDLVVAKALQEHGGIFGCEENGGMIFPDFVWG
                     RDGAMAAAQILHLMAKKGKALSELLRELPPYYQVKTKLEIDPQKRDRILKEVEKAITE
                     EKNQKEIITIDGYKVVFEDGSWLLIRFSGTEPLVRVFAESKDQEKAKKLVQKYLDLIK
                     GI"
     gene            23303..24931
                     /locus_tag="PK016_01195"
     CDS             23303..24931
                     /locus_tag="PK016_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01729.1"
                     /translation="MKRILCFLTLILLLFFSSGAEAYEILLYGNLTQPLQEDGVALQI
                     DLGGHQLKEEEWKNILDSSENTVLLPSLGDLEKGISFLEENKGKILGIVVSDSFAEEQ
                     SIFAPFLSFTHNYHRVLIFNFLQDNPEVPWDKYLEETEEVDLIIVVGTENNLPSLREK
                     FSVLGEKVQFIARGSSLDFQEKLSVEEKPVLFFFYSSRCPTCRRLKSEVVPPIVDKYK
                     DKIKVVYLDYLFSDNYERMVEWEEHWQVENKTSVEVFSDAGFVAEEDEKEFVAQLEEL
                     IQKTIESGKKKVSSPQEGENLIFRRFQGFTPWVIIGAGFLDGLNPCAFATIVFMVNLL
                     LLLGHSRRHILAIGLTYSIAVFITYLLLGLGLFQFWQVLSSYQTASRILYLIMALLLL
                     VFAALSIKDAIQYKREGKETGMTLGLPESWRVRINQYLKNSFTEKNLVVAAILSGFVI
                     SLLEAGCTGQVYLPTIMYIARESPYRWQALGYLLLYNSFFILPLLVVFLTIFWGSQSK
                     ALVNFARKNIVFSKIALAILFIFLSLLLLEGALF"
     gene            26180..26542
                     /locus_tag="PK016_01200"
     CDS             26180..26542
                     /locus_tag="PK016_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01730.1"
                     /translation="MKSTRKYVLLGLGVVLLLALLSGCEVTVGPEPPVYGSIRICVRE
                     GSVISGWVYIDGKDMDEHVDGWYGPYCTDWNRMTLDKEHLLEIRGSSRTWRTWFAPTR
                     EGQTFILDTHDGEHLIIY"
     gene            complement(26558..27226)
                     /locus_tag="PK016_01205"
     CDS             complement(26558..27226)
                     /locus_tag="PK016_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01731.1"
                     /translation="MKKVLNKRLSLLIMRTFIILGVIFITTSSLLSKETFNYKIIIRE
                     QEVGKMIYEVEEENGAWQIQSFIEVEGKREQDAQITISPDTYEPLHSQKWVRIPGGEI
                     VIETTYQEGKAQIKITSPEGTKEATISIPSPTFDSEEIPYLIGKLSYEEKAISVLVPI
                     SGMVWRGEIQKIAESEEEETFRFTLGGEITYLRYQKETPRLVELRAPQRGYNLVLEDT
                     SRRE"
     gene            complement(27223..27993)
                     /locus_tag="PK016_01210"
     CDS             complement(27223..27993)
                     /locus_tag="PK016_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01732.1"
                     /translation="MNYLWYIVITILIAFGLFVALFFRKTKFPRKKLVLYYLLSLILP
                     LVIPYRWLEKILFDYLAGLPSAIFISLVLILIEWALLLFLLMKANQETRETIEEKFFL
                     FLLLGLGLGLGKALRDTLLVYFHSLSTFYGYSQFANFPSFFYLLQKIMEAQIEVISAG
                     LLGLAFLSPRGWTFYNLVSYSLLSFLPKGLLGLAQVLIQFVPSLSFLASVYLISLLPL
                     TLIIGFGLLWHLYRRRLYQIIQEDEVPPIEASQEGSIK"
     gene            complement(27990..28631)
                     /gene="nth"
                     /locus_tag="PK016_01215"
     CDS             complement(27990..28631)
                     /gene="nth"
                     /locus_tag="PK016_01215"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HXL01733.1"
                     /translation="MTDENLGKAFAILQEIKGQWQDNTVNSLGSDPFLVLTSCILSHR
                     TKDQVTREATQRLRAVAPTPEDILKIPEEELSQIIYPVGFYRRKAKTLRKIAEILLTR
                     YQGEVPDDLEELLKLPGVGRKTANLVLTIGFHKPAISVDTHIHRIVNRWGYVETKTPE
                     ETEKELRKKLPLTYWEVINPLLVLFGQNICLPRRPRCFECPIEEFCEKRGVEN"
     gene            28662..30134
                     /locus_tag="PK016_01220"
     CDS             28662..30134
                     /locus_tag="PK016_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873143.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HXL01734.1"
                     /translation="MQVKIIVLLIYVVGMLLIGYFSMKKTKTIGDFFLANRSLGPWVS
                     AFAYGSSYFSAVLFIGYAGNIGWGFGLSSLWIVVGNTLIGSLLAWLVLARRTRLVTEE
                     MKVMTMPEFLQVRYGSNFLKAFSALLIFIFLVPYSASVYMGLSYLFDVVMGIPYVYAL
                     LLIAGLTGVYLVMGGYLAVNLTDFFQGSIMLGGAIFLVVYLVGRPEVGGWSQIVTRLR
                     EVNPQLVGVVGPPGFWPLFGLVVLTSLGPWGLPQMVQKFYALKNEKIVYTATIVATIF
                     AFICTFSAYLSGSLTHLFFDQLPQINGEPNPELLMPHLISQVMPPLFSLIFLLLVFSA
                     SMSTLSSLVLVSSSAITIDLLPARFRDKSLPLMRILCGIFIFFSLLIAFYRVTFIVNL
                     MSISWGAVAGSFLAPYLYGLYWKGANRWGAISSMLAGLAVSLGFSWYYGFNSSMAPLG
                     GSLAMLIPLVVLPIVSILTRGEESVSLRIREKGTVGNTGD"
     gene            30082..31092
                     /locus_tag="PK016_01225"
     CDS             30082..31092
                     /locus_tag="PK016_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HXL01735.1"
                     /translation="MLACVFEKKEQWAIREIEEPRINPDEVLVRVKAAGICGTDVHIF
                     RAEYFSDFPLVAGHEFSGEVVKVGSEVTQFKPGDRVTADPNIFCDRCYFCKINKNNHC
                     LNLQAVGVTRNGAFAELVAVPEKCVFHLPSNLSFSEGALTEPLSCVVYGVKRSNIQPG
                     EKVLIFGAGAIGLLLLSLFKVSGASQIAVVDVSEKKLNLAQKLGAQEVVLADGREGKR
                     LKEIAPRGFEIVVDATGIPQVMEKELEFVESDGTFLIFGVAPRNSVIAIDPYEIFQRD
                     LRIIGSFAVKKTMQYALNLLESGKVKVKDLISATYPLADFGKAMDDVLNNKDHLKVQI
                     VF"
     gene            31276..32280
                     /locus_tag="PK016_01230"
     CDS             31276..32280
                     /locus_tag="PK016_01230"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXL01736.1"
                     /translation="METFNFQWHITNRCNLRCHHCYQEKFQDEVEEGHLLLVAHRLME
                     ELEKRDLHTVINITGGEPLLLGEDLFSLLNYLNSHPRVGELMIITNGLLLEKDLLFRL
                     EEYQKLTTLKISLEGANSFTNDSIRGRGVFDKVVGKIKLTEKVSSLEVVLMFTLSKRN
                     VKEFSSMLTLAKNLGVEGVMVERFVPQGRGKEMREATLSREEWRSFLEELINICELEC
                     TPYDLLPYRAFWIKGEDEAEVLGALCNLREALCLMPEGTIFPCRRFPYPLGNILEDGL
                     GKAIGSPFLETLKDRKQLKGRCGRCQVEGCYGCRALGYALFNDPYYEDVQCFWEETMP
                     "
     gene            complement(32270..33226)
                     /locus_tag="PK016_01235"
     CDS             complement(32270..33226)
                     /locus_tag="PK016_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HXL01737.1"
                     /translation="MPANLTPQYLEAEARYRSARTSEEKLKALEEMLAVIPKHKGTEK
                     LQAEIKQKISKLRKSQGERKGKGKLFDPYLIEKVGAAQIAIIGTPNSGKSTLLSLLTR
                     AHPEIADYPFTTHSPLPGMMEYQDIQIQLIDFPPWLEGKIEGNALSALRRADGVIVVM
                     DGKSERVLEEMEGLLEDLEESKFELEKERSLILVNKIETPLEQETFSIFKELYEARFT
                     IQGISLLTMSAEEKENLKRDIFYLAAIIRIYSKPPGKPPDLSRPFILKKGETVKDLAC
                     AIHKDLVHTLKGARVWGSAKFEGQLVTPDYILQDKDIVEIKA"
     gene            complement(33233..33973)
                     /locus_tag="PK016_01240"
     CDS             complement(33233..33973)
                     /locus_tag="PK016_01240"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HXL01738.1"
                     /translation="MKKCLWVIVILIVGISFILYLQSRSPKSNSFSMEGWEDKCIKMV
                     EQQIKKRGVKNPLVLKTMQEVPRHEFVPENLHNQAYDDTPLPIGYGQTISQPYVVALM
                     TENLQIVPGCKVLEVGTGSGYQAAILSKMGCQVYTIEIIKDLAKTAEKKLKELGYDNI
                     TVKQGDGYLGWEEEAPFDRIIVTCAVDHLPPPLLAQLKEGGKIVIPVSPPWNIQSLWV
                     VEKTEEGITTRDLGAVRFVPLTRESQGE"
BASE COUNT         9712 a         6171 c         7829 g        10580 t
ORIGIN      
        1 cgggagtata ccggcggata ctctcaaggc acattttagt atattcaagg ttattacggg
       61 tgaggatgac gatagaggtg agactcaagg ttttcggagt tacctcttta ggctgagcca
      121 ccaaaagata ttgatatatt cgtccttcct cttctaaagt gaacaatttc attcctgaag
      181 tttttaaagc atgcaaaaaa cctggtggta cttccgctcc ttgcaagata gttccttgta
      241 agttagtagc tttaaatcct gttttatcca gcatatccag aatagttttt ttagtgaaaa
      301 atcttaaatg agttttatct aaaattccag cttcttggta gtcccacctg ccctccaaaa
      361 ggtcttttat aaccgaccaa tgcctcacat taggaacact aattatcaca ataccttgtg
      421 cgtttatgcg agtcttgatc tggctgagaa tcttccaggg gtttttgaga tgctcaagca
      481 catcagcaaa aataaccaca tcataatagt tctctggcag gtcatctatt atttcttcaa
      541 tatccccaac tattacctta tccagcttct ctcttgcttt gtcggccacc tcaggtacaa
      601 tctctatccc tgtcacttca caatcttgcc tttcttttaa ggctttacct aactcacccg
      661 ctccacagcc aatatccagt atttttttgg ctgaggaagg aataagggct tgaatctcag
      721 gacgagaata agaataataa attaagctct ttccttctga taaataaagc tcttttctct
      781 ttagaggatt ttgaactata gcgatgaaag cgagagccct ttctattaat acaccattgt
      841 ccgttcgcaa ttctaacttc cccgaagaca gatactgacc aatgaattta aaattgtgga
      901 agtatttggc aagtatagac ctaaactctt gataagagta ttctctaaca tggaaaggat
      961 tgccaggttc tttatctggg gaatatacag ttttattggg ggtagagaca atcagcatcc
     1021 catcaggttt gagtactctg cttaattcct ttaaaagtac atcagggtca tctatatgct
     1081 caaatacttc aaaacacacc accacatcaa atttgttgtc ctcaaaggct aaatccagca
     1141 cattcatagt ctcaaaagtt aaattatcct tttggtactt ttccaagtcg tattctatta
     1201 tttctcttgc ataatctata ccaataactt ctcttgctcc cccttcggta gccataatcc
     1261 agcttccata tccagctcca caaccagcat ccaatactac tttatctttt acaaactggg
     1321 aagcaaaccg gtatctttga aaatgcatag cctcagtgag tgaatcagat ttcccgggaa
     1381 acattctctc actttttaac ttaaactctt cccagtgatt agcttttatc tctgaaaata
     1441 cagcttctac ttcattttct actatatccc aattgtactt tttcgccact tctaatcctc
     1501 gtttttgcaa tctggtatat aaatctttgt cttccaaaag atggataact gcttgagcca
     1561 tagtctcagg gtcttggggt gggacaaaaa gggcataatc tgtaacctca tcataactac
     1621 gataagcgga tatatctgtt aataccaccg gagttccaca tgccatagct tcgatgggag
     1681 gcaaaccaaa cccttcttcc ttacccttgg aagaagccat taatacatcg caggagctat
     1741 aaacttttgc catctcttca cctgacaaat gattataata ttcatcgacg actcctaaga
     1801 attgttcttc ttgagagatg ggaaattgag aaactctgat aatctcaaca gcattgccta
     1861 actttgtctt taaaatttta gctgccttta gcgctgttct tatatctttc cactctacct
     1921 ctattggccc tactattaaa actctctttt tagggaaagt ggctctattt tcggggtgga
     1981 acagagataa atcgatggca ttatgaatca tataagtggg atgatgatat ttctcctcca
     2041 gaattttttt caaaaataaa ttattaacca ctttaaccgt ataaagacta taaacaaaat
     2101 ctatataatt cttagctcga ggatcatcag ttccaaaata ttcataacct tggcaataat
     2161 ggataggaat accaaaccca gcatcgtgag caacgacaac tgtgggataa aaagtaccta
     2221 ttataagctc actatcttgt ggaataactc ggggggtaag ttttggaaca ctaataaatt
     2281 caaaatctat tttaaaccaa tctggtttgt cttttactgc aataaccctt accctgtgtc
     2341 ctcttttatg taacctgcta gcttgttcta aaggaatttt aatacctccc caaagagaag
     2401 tatctggaag aacataagtt atattcattt ttaatcgccc tatataagtt tattaactat
     2461 atgataaaaa aaattgccaa acttaactgt agaattcaat tatgttccct taaggaggag
     2521 ctctcaaaga cacccttacc ttaatcttct tccgtcaaag gagagggaac tctttaaaga
     2581 gaagtacaaa gaaataagca aatttgctat aatctggaaa atgggtggta aaaaggaatc
     2641 aggcaatttt ctcaaggggg taaacaatga aaggtttaat tttagctgga gggactggct
     2701 cacggcttta tcctctcact aaagtgacca ataaacacct tctcccggtg ggaaggtatc
     2761 ctatgattta ttatcccatc tatcacttaa ctcagatagg gattcaggac atcctggtag
     2821 taaccggcac cgagcatatg gggaaagtgg tccagctttt gggaagcgga cgagactttg
     2881 gagtgaaatt tacttacaaa gttcaggatg aagcaggggg gattgcccaa gctttaggaa
     2941 tggggaggga gtttgtggga gaagataatt gtctggttct tctcggggat aacatttttg
     3001 aggatgacct cactctttat tttactcact ttcaaaacca aaaaagagga gcgcgcctcc
     3061 ttttaaagga agttcctgac ccggagcggt ttggagtgcc agtattcaaa gaggggaaaa
     3121 ttatagctat tgaagaaaag cctacttctc ctaaaagctc tttctgtgta gcggggattt
     3181 atttttatga taatcgagtg tttgatgtta tctctcagct taagccttcc agtcggggag
     3241 aattagaaat cactgatgtt aataactggt atatcgagag gggagaggca gaatacgata
     3301 ttttatccgg attctggact gacgccggca catttgaatc tcttagtcga gcaaatgagc
     3361 tgacccggga tatgactctc gattttggga gggagtaaaa ttggtgaaat ttacccctgg
     3421 agatataaaa ggagtaataa aggaagagct tgcaagatat gaagataaac ggggatggtt
     3481 gatagaagtt ttccgccagg atgagctcct tgaggactta tatcctctta tgggttatat
     3541 ttctctcact caccccggag tcacccgcgg tcctcatgaa caccgctttc aatgggacca
     3601 ttttatattt ttaggttctt ctgtttttcg tttatttctc tgggacaatc gtccttcttc
     3661 ccccacttac cagaaccagc ttatactgat tgccgaggaa gaccatcctt tacgagtagt
     3721 tattcctccc ggagtagttc atgcttatca gaatcgggga gaaaaacccg ggttagtcct
     3781 taattttccc aaccagcttt atcgaggatg gggaaagaaa gaagaagtag atgaaatccg
     3841 ctgggaagaa agaaaagact ctccctttgt ggtagagtaa cctatgaagc tcctcatcac
     3901 cggttctcag ggtcagctgg gaagagagat tcaaaaagaa ggagaaaaaa gaggatatca
     3961 ggtttatcct ttttctcatg aagaacttga tatcactaaa atatccgctg tagagacagc
     4021 tgtccaggag ttaaaacctg atatcatagt gaactgtgct gcctataacc aggtagacca
     4081 ggcagaaaaa gaaagagaaa cagcctggaa agtaaatgtc gaaggagtag aaaatttagc
     4141 tttatcctcc cggaagcacg ggagtttttt agttcactat tccaccgatt atgtattttc
     4201 gggaaaccag agtcatccct accgagaaga agaccttcct tctcctctca attattacgg
     4261 gaagacgaaa tgggagggag aaaaagcgct ggagaaggtt ctttctcctt ctcagtttct
     4321 cctccttcga gtaagctggg tatttggtaa taaccccaag gtaagctttc ctttaaagct
     4381 cctccagtgg tcgagggaga aacaaactct ccggatagta gatgaccaga tttcttctcc
     4441 cacttatgct cacgatgcag catattttac tttagagcta ttagaaaaag atgcccaggg
     4501 gctttttcat tttgctaact ctggatattg tagccgatac cagtgggcta aattcatttt
     4561 agaaaagcta aactggagag ggcaactttt tccggctaaa agtagcgatt ttcctacctc
     4621 tgctcagcgg cctccttttt ctgcgttaga ttccagaaag gtggaaaccg tcttgaaaag
     4681 gaaaatacct tcctgggaag aagcaactga ccgatttctt ttatcgttga aggagggaaa
     4741 aaatgaatag aattttaatc agtggtggag caggtttcat tggagccaat ctggttaaac
     4801 tgttgctgga atgggagcca gaatggaaga tagtggtggc agatgctctc acttatgccg
     4861 gagataaaaa gaggttagaa gaaatatccg ataatccccg gttttctttt tatcaagtag
     4921 atatcactca aaaagaaaag atgagagaaa tctgggagag ggaaaagcct cagggagtaa
     4981 tccacttagc cgctgaatct catgttgacc ggagtatcaa agacccctct ccttttattt
     5041 taactaacgt tttaggaact gaagtacttc ttgagttatc tttagaattt ggggtgtctc
     5101 gttttctcca tgtctctact gatgaggtgt atggagagtt aaaagataaa gagggtaaat
     5161 ttaccgaaga acatcctctt gctccttctt ctccttattc agccagcaaa gcagggggag
     5221 agcttttagt taaggcttat gaacggactt ttaatttacc tattgttatt gttcgaccct
     5281 ctaacaatta cggtccctgg cagtatcccg agaaattaat cccggtagtt atctggcaag
     5341 cactccatca tcagcctgtc ccggtctatg gtcgaggaga aaattggcga gagtggctct
     5401 atgtggaaga ttgtgctcaa gggatttatg ctgcttttct taaagggaga gagggagaaa
     5461 tatataactt gggaagcggg gaagaaagac aaaatctgga tacggtaaaa acaattcttt
     5521 ctcttctggg agaaaaagaa gaccttattc aatttgttcc cgaccgaccc ggccatgatt
     5581 tccgctattc tttagatagt caaaaagcag agagagaact aaactggcgg gctaaaacta
     5641 aatttgcaga ggggataaag aagactgtag actggtatat taagaatatt aaatgggtag
     5701 aagagaaaat aaaggagata aactccttat agggatgaaa aataatcggg ctggaaataa
     5761 agtaatttgc agtgagataa tctaataagg gatgagcaaa ttaaaagttg gagagatagt
     5821 agattagatc ggagaaagga cagctaataa aagcaagacg agaaaagata ttggtccttt
     5881 tttttgtctt gcttgaatgc ctctatcatc cccttgtctg tcattcctga atggttctat
     5941 caggaatcca aattatttaa gggcaaaaac ttttggattc ccgattgaag atttcgggaa
     6001 tgacggaaaa ggaggtcgtc accccggcga ctttcgagcc ggggtctata ttatttaaga
     6061 acaagaatat ctggattccc gataacagat ttcgggaatg acgaatgaga gagttcctcg
     6121 actctttttt tctttccctc gcccttgaag ggagagggtt agggtgaggg tgtctttttt
     6181 cggccattcc tgaatgtctc tatcaggaat ccagacaata aggaggcaaa aaccccctta
     6241 taactcccaa aaaaacaaaa gaaacaaaaa cttaaaaacc caagattact ggggaaaaaa
     6301 cgtccctcag tagatgcttt gccgctgatt taggggatga tgagtaagaa ggaatggcag
     6361 gcgagaacta taaaaaggga aagcacttct cttatagttt tatgctttct atgcctttat
     6421 gcagagggct atggataaac gtccctgaca ataggttcta tagttgattt ctattacagg
     6481 attgaaattg atatcttata acttgagagg atagaagttt acaaaagtat actttcttgt
     6541 ttcttaccac aaggattttc ccctccccca ttgacaaaga gtccagaaat tcctgcaagt
     6601 tagaaaaaga tgtgataaaa tggggataaa gtttctttaa ggagttgaaa gtatgtctgg
     6661 acattctaaa tgggcacaaa taaaacacaa aaaagccaaa actgattcgg ctcgaggcaa
     6721 agcttttagc aagttgattc gtcttatcac tgtagctgct cgtcaggggg gaggcaaccc
     6781 ggagaataat gctcgcctgc ggattgctat ccaaaaagct aaagaaataa atatgcctca
     6841 agaaaacatt gaaaaagcga ttaaaaaagg aactggagaa ttagaaggag taaattacga
     6901 agaaataacc tatgaggggt atggtccagg aggagtagcc gtgatgatag aggttatgac
     6961 tgacaatcgc aatagaacta cagcagagtt gaggcatata ctttctcgtc atggaggagc
     7021 cttaggagag agtgggtcgg tttcctggat tttcgagcgc aagggtttta tcagctttga
     7081 taaggaaaaa gtggacgaag aagagttgat gaccgtggct attgaagccg gagcagaaga
     7141 cttgaaagta ggagaaagca ctatagacgt tattacttcc ccttctgatt ttgaccaggt
     7201 tagagaagcc atagagcgtg ctggtctatc ttatgattta gctcagatta ctatgcttcc
     7261 taaaactatg gtcaatgtgg aggggaaagc cgctcaacag gttcttgagc ttattgatgc
     7321 cctggaagag cacgatgatg tgcaggaagt ttattctaac tttgaagtag ctgatgaggt
     7381 tctggaatct ctgaacagct aaatgtccga gagtacctgt atattagggg tagacccggg
     7441 gactgccatc actggttttg gaatcataga gtcttttgct cagggtaatt ctctgagagt
     7501 ggtagactgt ggtttcattg ctactgataa agggcttcct gctagtgcga gattaaagct
     7561 catttttgag gatttaaaga ccgtaattga aaagtttgct cctcaagaga tggcagtaga
     7621 agaattgttt tttaataaaa actctaaaac tgctatttct gtgggagaag cgcgagggat
     7681 agttttatta tgtgctgctc tttatcaggt gccggtctat gagtataccc ctcttgaggt
     7741 caaacaatcc gtagtgggtt atggtagagc cactaagagc cagattattt atatggtaaa
     7801 acagattcta aaaatagaga aggaaataaa tccggatgat gtggctgatg ctttagcagt
     7861 ggctatttgc cacgccttcc gttcggggat gtagaggaaa tgatagatta tcttagagga
     7921 aaagtggtag aaatggatga ggaagattgg gtggtagaga ctaataactt tggtttccgc
     7981 ttaaaagtta ccccttttgt tttgcccggg aatattggag aagaaagatg ttttcttttg
     8041 cgcattttta tgaaggaaaa tggagaattc attttttatg gttttgataa tgaagaagaa
     8101 aggtcggttt ttgaccagct ccgggaaatt aggggggtta atcatcgtac cgcttttaag
     8161 attctctccc gagtaaagtg ggaagagctg gttaaattga ttctgaatga agagatttca
     8221 gttctggaga ccagaaccag tctcagctcc aagactataa agagattagt tttagagtta
     8281 aaaccccgct ttttgaaaaa aggttggaag atagagggtg gggagcgctc aggggaagta
     8341 gtagaagaag ttagagaggt tttatcccgc ctgggttatc gcacggtaga aattgagggg
     8401 gtaattgatg acctgtgggg tgagcttggt agagaagggg cagatgtaga aacctggatt
     8461 aaagaagctt taatcaagtt aggtggtgga gtttgaaaag agagtcagtg gatgattttc
     8521 ttatccggcc gcagagtttg gctgattttg tcggtcagga aaaagttaag tccaatctcc
     8581 aggtttatat tcaagctgct cgctccagag gtgaaccttt agaccacgtg ttgttgtttg
     8641 gtcctcctgg cctcggtaag actaccttgg ctaatatcat tgcccatgag atgaagacca
     8701 gccttcgtta tttatccggt cccgccttta cgagaggtgg agatttggct tctgttttca
     8761 ccacactttc tgcaggagaa gtagtgttta ttgatgaaat tcaccgtctt cctcctcctt
     8821 gtgaggaaat tctttatggt gctatggagg attttgctct tcatctcgtg gtgggaaagg
     8881 gtccgggagc gaggagtata cgcattcaat tgcctctttt taccctggtg ggagctacta
     8941 cccgggtgag ccttcttagc tctcctttgc gtaatcgctt cggcattgtg gagaagctgg
     9001 atttttacca ggaagaggaa attactaaaa tagtagaaag agcggcacag gttatagggg
     9061 tggaaattca accccgggga gcagaagaaa ttgctcatcg ttctcgtggt actccccgaa
     9121 tcgccaaccg attgcttcgg agagtgagag atttcgctca attctttggt gaggaaataa
     9181 taaatgaaga aatagcaaag agagctttta cctacttagg agtagatgaa gtaggtctca
     9241 ctgattttga ccggagattt ttagaagtgc tggttaagta ttatcaagga gggccagtag
     9301 ggattgaaaa tatcgccagc attttaggag aagatcctgc tactctggag gaagtttatg
     9361 agccttttct tctcaaatta ggttttattg cccggactaa aagaggaaga ataataactg
     9421 ctcgaggtcg tcaatatctt caaaaggtga aagaagttgg ctgataaagt tttactgcat
     9481 acctgctgtg ctccttgtgc tacctatgta aacgaagcct tagaaaagga agggtgggaa
     9541 gtggtaaata tcttttataa tcccaacatc catccttttg atgaatatgc tcgtcgttat
     9601 gaaactctgg ttttatattt agaaaacgaa ggtaaagttc tcgaagctcc ctttccttac
     9661 aatcctctgg aatatttttc tttgattacc gagccagaaa accgctgccc taaatgctat
     9721 tttctgcggt tgaggaaagt ggctcagtgg gggaaagaaa gaggctttaa attttttacc
     9781 accactttga ctatcagtcc ttatcaagat ttaccagcca tctgggaagt ggggaaacaa
     9841 attgaggaga aactggatat taagtttctg attcgagatt ttcgagaagg tttccgccag
     9901 agtgtggccc ggtcttcaga actgaagctt tatcgccaga attattgtgg ttgtattttc
     9961 agtaaatatg aaggagcgaa aaggaggtta gaaaaacgtc ttactgggct aaagttttct
    10021 taactctggg tctcgtgttc atagtggtgg gagttctatt gctcctttta tcccggattc
    10081 cgggggtaag taagctggga catctccctg gtgatatagt ttatcggcgg ggtaatttcg
    10141 tcttttattt ccctcttatg acctgtctta taatcagcgt gattttaacc cttattttta
    10201 ccctattatt caggcgttag gaggaagaga tgctgaaaaa aatcgtcctt attctctgct
    10261 taattctttt tgtaagtcta aatgaggggt ggggaaaaga attagaagtg acagttaatt
    10321 tgaacaaggc aaaagaagca gtatttagct catctggagg attgaaggta gaaataggaa
    10381 agcgttcttt ttctgttcct ccttcttatc ctgttcattt ccgggaagag ggggaggaga
    10441 taaaatggga agaagagaac ctcctttctt ctgaggttta tatatatcct aaaggtaagg
    10501 aagcagtttt gtggggcaaa agaccctatc gggggagttt tattataaaa gcatcagacg
    10561 gaggtatttt agtgataaat aaacttcctc ttgaggatta catcaaagga actatgaagc
    10621 tggaattaag tccttcctgg cctgtagaag ctctcaaagc tcaaatcata gtttctcgca
    10681 cctatgcttt gagaaatttg cggcgccatg aggaggaagg ttttaatttc tgcgctactg
    10741 aacattgcca gcgttacggg gggattaacg ctgaagaccc ggttaccaat cgcttggtgg
    10801 aagaaactaa aggtttggtg cttacttatc agaacgagtt agcttcagtc gtttttcact
    10861 ccgaaagcgg cggtttcact gaaagtgcct ttgatgtgtg ggggagggaa gtaccttact
    10921 tgatagaagt ttcctctccc tgggaagaaa atgctcctca ttcatcctgg gaggtggagt
    10981 ttaaaaaaga agaggtagaa aattgtctta ataaagcagg cctcatccag ggagaaattc
    11041 tggatattaa cttatcttct tctccggagg gaagagtgga ggaagttgag gttataacct
    11101 cgcaagggtt attttccttt cctgcttctc gatttcggga agctttaggg tgggagaagc
    11161 tccccagcac tttttttgta atgagaaaag tggaaaaaga acagaaagtg agaaaagaaa
    11221 cctttccttc tccttcttcg tcatcggcct cttcttttga ttacgaagag tggatggata
    11281 aagattggga tttagatgat ataattaact ttttgaaaat gagagaagaa gaaagaaata
    11341 ggaagaaagg catttcttct cctccccgga tagtggtgga ggaagaagtt atccctcagg
    11401 gtactacctt tatatttgag ggaaaaggtt atggtcacgg agtgggtctt tctcagtggg
    11461 gagcaaaagg tatggcagag gaaggttttt cttgccagga gatacttaat cattatttcc
    11521 ctggctgcaa gatagagagg gcaactttta agtgagtggt ctggtttctg aatttgattt
    11581 ttctcttcat ccggagctga ttgctcaaga acctttttat cctcgagacc actgtcggtt
    11641 gatggtggta gaccgggagc aaaaaacctg ggaacatcgt tttttctatg aaatagtgga
    11701 atatttagag cctccagatg tcctcgtaat aaacgatacc cgggttttgt cggctcgttt
    11761 ttgggtctat aaagaagaaa ctggaggaag agtagaaatc ctatttttgc gccaccgggg
    11821 agaggaatgg gagtgtcttt tgcgtccttc ccgaaaagta aagactggtc aagttctcaa
    11881 attggaaaaa gaccctcgtt ttagctggag agtaagagag cgaaataatg ggatatggtt
    11941 aattgagcct cttttttctt cctcggaaga agagaagtta tttgactctt ttggggaagt
    12001 acctactcct ccttatgtta aaaaccgggc tataaatctc gctgattatc aaacagttta
    12061 tgctcgtatt cccgggtcag tagctgctcc tactgccggc ctacatttta caccccattt
    12121 attaaagaaa ttagaggaga aaggggtaaa aatagtgtcc cttactcttc atataggatt
    12181 ggggactttt cgaccggtta aagaagagag gatagaagac cataatatgc actccgagca
    12241 ttttttgcta accgaaagcg cagcgcagac catccttgaa gctaaagaaa aaggagggaa
    12301 agtagtagca gtgggaacaa cggtagtaag agttctggag gcagtctggc agcaatttaa
    12361 caccttaaag ggtagggagg gagaaactaa tctctttatt tatcctggct ttcattttca
    12421 agctatagat tggttgatta ccaattttca tctccctcgt tccactcttt tcatgctggt
    12481 ttgtgcttta gccgggactg aatttatgaa agaagcgtat gaggaagcta ttaaaaaggg
    12541 ttatcgcttt tttagctttg gagatgctat gcttataaaa taagaggtga agatttggga
    12601 gactttgaac tcttagttag tgattctcgc tcgagagctc gcctgggatt actgcatacc
    12661 gcccatgggg taatagaaac accggttttt atgccggtgg ggactcaagg gacagtgaaa
    12721 gctatttctc ctgaccgttt agaagaaatg ggagtaaaaa tttttctctg taatacttat
    12781 cacctccatc tccggccggg agaagatata gtaaaagaag cgggaggttt acattctttt
    12841 atttcctggc ctcattctct tttaactgat agcggggggt ttcaggtgtt tagtttgact
    12901 gatttggtgg aagtggatga tgacggagta tattttcgtt ctcatttaga tggttctctt
    12961 catcgtttta ctccggaggt ggctttgcag attcagatgg atttgggagc agatataata
    13021 gtaactctgg accagtgcat tggttttcca gcttcttctc accaagagcg cctcggagca
    13081 aagagaactc ttcaatgggc gaaaaggagc aaagagtttt tttcttctcc tcatcagctt
    13141 ctctttggta taattcaggg agggatggat gaggatttac gaatttgttg tgctaaagag
    13201 acggtagcaa tgaattttgc cggatatgct ctgggaggtt taagcgtggg agaacctcct
    13261 cctcttactt ataagatgat tgaaagagtg gaaccttttt tacctcctga taagcctcgt
    13321 tatttgatgg gggtagggac gccggagagt ataatagagg gagtgcgccg aggcatagat
    13381 atgtttgatt gtgttcttcc cacccgtaat gctcggaatg gttgcctttt aactcgcaga
    13441 ggtaaaatta acattgaccg gcaggaattt tcccgggatt tccgtcctgt agatccggag
    13501 tgccattgct atacctgtct caatttttct cgagcttatc tgcgccatct gtttaaatgt
    13561 aaggaaatat tagctagtga actggctact atacataatt tatattttat ggtagagttg
    13621 atggaaaata ttaggaagag tttaagagag ggttattttc tcgattttgc cgaagagttt
    13681 ttggctgagt atgagagtaa tcctttctct tctttttcta aaaaataaga aaggagtgat
    13741 gaaatgatga actggtttat ttctctggct catgctgcta atgaagtacc tgcccccgct
    13801 acacctgcta cacctgctgc acccagtatt attgcccagc ttttccctct gatttttctg
    13861 atcgttatct tttattttct cctcattcgc cctcaacagc agaggcaaaa aaaacaaacc
    13921 gagttgttga atagtattca aaaaggagat aaggtagtaa cgataggagg aattcatgga
    13981 acggtaaccc aggtggatga tgaggcagta ttgctggaag tagataaaaa tacgaagatt
    14041 cgcctttcca agagtgccat tgcaggtaag gaatagtttg tgagctaagc tgattgggga
    14101 ggaaaagaag ttgagaagaa gtaatttacc ctggagattg attatatcta tagccttagt
    14161 agtggtatct ctggtggtta tttttccagt aaggcaaaaa attcggttag gtcttgattt
    14221 acagggaggt tctcatattc ttctggaatg tgtggatacc cccgaagctc ctgtagatag
    14281 tgatgcggtg aacagggtgg tagaaatcat ccgtaaccgt gtagaccagc tgggtgtagc
    14341 agagccggtt attgcccgac aaggagagcg ccgagttctg gtacaattac ctggagttac
    14401 tgacccccag agggcggtag aggtaattgg acgcactgca cttttagagt ttaaagatga
    14461 agaaggcaac acccttttaa ctggagctca ccttaaaaat gctcaggtgc aatatgaccg
    14521 ttttgggagg ccagtagttt ctatagcttt tgatgaagag ggagctaagc tttttgccca
    14581 ggctactact actaatgtag ggaaacctat aggaatttat ttagatggta atcttatttc
    14641 taacccggta gtgcaggagc ctataatggg aggagaagca cagattacgg gaagattttc
    14701 tttagaagaa gcacagaatc tggccatatt gcttcgagct ggtgccttac cggttaaagt
    14761 agatgtgata gaaaaccgct ccgtagaacc ttctttaggt caggattcta tcatttatgg
    14821 actccgagca gcggtagttg gtgctctcct ggtcgtggca tttatgatat tttattatgg
    14881 atggctggga gtgctggctg atttagcttt ggcttccttt actgtctttt ttctggctct
    14941 ttttattagt ttgcgggcta ctttgacttt accaggaatt gccggtttca tagttactgt
    15001 gggtatggcg gtagatgcca acgttctgat ttttgagcgg attaaagagg aatatcggac
    15061 agggaaaacc tggcgagctt caatagaggc agggttcaat aaagcctttc gcaccatttt
    15121 agattctaat attaccactt tgattgccgc agctatgatg ttttatttag gatccggtcc
    15181 ggtgcgaggt ttgggagtaa ctctaacttt aggtatctta tgtagtatgt ttaccggtat
    15241 ttgggttact cgagctttag tagatttttt tgctgaacgg atcaggttta aagcttgaag
    15301 agccaggagg taatataagt ggaattaata aaaaatccta actttaattt tgtaggaaat
    15361 cgccgtatag cctatgctat ttctattgtt atcatagcgg tggggttgat tagcttactg
    15421 gtgcagggac taaactacgg tgtagatttt gttggaggaa ctcttctcca gtttcggttt
    15481 aaccgggaag tggaagtgga agaagtgcga gaagctttag aagggttaaa tctggcgaaa
    15541 agtacggtgc agcgtttttc tcctcaagag gtaattatcc gctcccctaa actcagtcaa
    15601 gaggaacagg accaggtaga aaaaaccctg ggagaggcat ttggtgaggt aaccctggta
    15661 agggtagaag atgtggggcc agcggtaggg gcagatttac gtcggatggc aattgttgct
    15721 gttttggtgg ctcttttggg gattctcctt tatgtatcta tacgttttga attccgtcct
    15781 ggagttactt ctattattgc tttagttcat gacgctttgg tcactttggg aatgttatct
    15841 cttctccagc gagaatttag tgccccagtt ctggcagcta ttttaactat tttgggttat
    15901 tccatcaatg attctattgt agtaatggat agagtgaggg aaaatctacg ttcccggaaa
    15961 aaaggagaag gctttggaga attggttaac cggagcatta atgaaactct ttcccgtacc
    16021 attaatacag ttttgaccac tattttgccc gtcctggctc ttttaatttt gggaggagaa
    16081 atgcttcggg atttttctct tactctattg attgggctta tcgctggtac ttattcatct
    16141 atttttatag ttagttccct gtgggtagag tgggaagaga aaaagccttt gcggaggtaa
    16201 ttaactttgg gcaagaaatg gcaaattaga aaggtgaatg aaggttattt aaagaattta
    16261 tctcgttccc tttctatgcc tctttctatt gctcgagtgt taatgaaccg aggagttttt
    16321 actcttcaag aggcacagga ttttttagaa cctcgtcttt cttttttgag aaaactcgag
    16381 gagttaccct atttgaaaga ggcagttttg cttttagacc aggtaattag aaaacaagaa
    16441 aaaattctta tttttggtga ctacgatgct gatggcttaa ctgctgcagc tattctggct
    16501 cgctttttgc agcttttttc tcccgaagta gaaatttata tacctaaccg ttttcgagaa
    16561 ggctacgggc tgggtagaga ggcggttaaa gaagttttaa agcgagagcc ccatcttgtg
    16621 gtaacagtgg attgtggtat cagagataga gagggtatag caactctaaa aagccaaggt
    16681 gttttagttt taattactga ccaccacctc cctgattttg acctctggcc tcagggggtg
    16741 tgcgtagtga gcacctggga tagggattcc cgagagatga tttctcccct ctctggagtg
    16801 ggaatagctt tagctttagc tcaggcttac ggggaatacc ggggggcaaa aataaacccg
    16861 gtagaagagt atcttgattt agcctgtata ggaacggtga gtgatgtagt tccccttttg
    16921 ggggagaacc gggttattgt tcgttacggt ttagagaagt taaaccgtaa tccctgttgc
    16981 ggtcttcggg ctcttagtga agtttctggt ctggctggaa aaacaatagg aacgcaggaa
    17041 gtgggtttta ttttagctcc tcgtatcaat gctgcgggtc gagtagaaga ttctcaacca
    17101 gctttggatt tgcttctgag cgataaatat gaagaggctt tagaattagc aaattatttg
    17161 agtactttaa attatcgcag gcaaaaagaa gaagaaaaag ttttagaaag tatttttcag
    17221 gatgagaaaa atgaagccta tcttcaagat gaagtagtgg taatctcagg atcagggtgg
    17281 agtcagggag ttttaggtat tgtggcttcc cgtcttagtg agcaattaga taagccagtg
    17341 atagttttaa ccgaaattga cgggatggca atgggttcgg gacggagtat taccggcttt
    17401 agcctcttct cggttttaga gcgcagctct tcttttctgg tccgttacgg tggccatgag
    17461 atggctgccg ggctccgtct ttttgccgat aaagtagagg ggctgagaaa ttttttaaac
    17521 gatagttttg cggaggaagt gaaaaaagct cggtctctgc ggggattagt agtagatgct
    17581 ctggtgggct tacaggaagt caacgaagaa actcttttct ggttagaaaa acttaagccc
    17641 tttggagaga agaatcctcc tcctcttttt gctgttctgg atgcctcttt gattaagagc
    17701 tggatatggg gaaaaaatga acagcacctc cgcttgctgg taaaacaagg aagagaagct
    17761 caggagatgg tggtaattaa tggtaagaaa aaggcaggga acctggttaa ttcttctctt
    17821 gtagaccttg cttttgaagt acatcgcgat aactcaggaa attatcctta tttaaaaatt
    17881 caggattgga ggatcaagaa gtgaaagaag tggacctaga cgtagatctg gctcaataca
    17941 taaggaatat tcccgatttt cctcagctag gggtgcaatt taaagatatt accaccttat
    18001 taaaggatgg catggctttt cgagagacta tcgaaagatt ggtaggaatt tttgaagata
    18061 aaggagtaga attggtagtg ggtatagaag cccggggttt tattataggt gcacctctgg
    18121 cttattctct gggttgtggt tttgtaccgg tgagaaaagc aggaaagcta cctgctccta
    18181 ctctttccaa aacctatgac ctggaatacg ggtcagctac tttggaaatt catcgtgatg
    18241 ccatagagaa agggcagaaa gtgataattg tggatgacct tctggctacg ggagggacta
    18301 ctaaagcggt ttgtgagatg gtagaagagt tgggaggtaa aatcataggt attggttttg
    18361 tcatcgaact ggaatctctg aaaggtagag agaaactatc ttcttaccct gtctattctc
    18421 ttatccgctt taacatttaa ccttccggga atttttgaaa gacaggtttc gttgtaaaaa
    18481 taagaaaatt ggggaggttg gtttctttgg ctggagtggg gtagagacta tagaatcttt
    18541 attagataaa atcaaccttt accatcctga ttttaatagc gatttaataa attcagcctt
    18601 ttctttttct cttgaggctc atcggaacca gactcgttta tctggggagc cctttattat
    18661 tcatccttta gcagtggctc atattgttgc tgatttaaga atggatgaaa atactattgc
    18721 tgcctctctt ctccatgatg ttttagaaga tagcgaagtt accgaagaag accttaaaaa
    18781 gaattttgga gaagaagtca ctaatctggt aaagggagta actaagttaa accggggatt
    18841 tagttatgtt agtcgggaag aagcggaagt ggagaactat cgccgtcttt ttattgctat
    18901 ggctcaagat gtgcgagtta tcataatcaa actggctgac cgcctgcaca atatgcgtac
    18961 tcttaagtat caataccggg ataaaaagga aattatagct cgggaaaccc tggaaatttt
    19021 tgctcctatt gctcatcgtc tgggaattaa taaaattcaa acggaattag aagacctcag
    19081 cctctttttt ctagaccccg gagcttattg taatattcag cgaggtatag agagagtcaa
    19141 agaggcacaa gaaaaacaga tagaagaagc aaaaaaggag ttagaagttc gtttagaaaa
    19201 agccgggatt gaagctgaaa tacaaagtag atttaaacat atttatagca tttataataa
    19261 gttaaatcgg cagaaaatta gcttagaaga cctacctgat ttaattgctc ttcgaataat
    19321 tgctcgagaa gtagagaact gttatgccgc tctgggaata gttcatactt tgtggaaacc
    19381 ggtggagggg agatttaaag attatattgc tgttcctaaa tctaatatgt atcaatctct
    19441 ccacactaca gttataggtc ctcgaggtgc gcctatggaa gtgcaaatta gaacctggga
    19501 tatgcatcag gtggcagagt atggagttgc tgctcactgg cgttataaag aaggtaaaac
    19561 tggagaaagc gatgacaaat ttgatgagcg gatgaattgg ctccgtcaaa tattagagtg
    19621 gcagcaagac ctgagaagca ctcaggaatt catggagagt ctgaaaatca gcttatttga
    19681 tgacgaggtg tatgttttta ctcctaaagg agacttaaaa aaacttcctc agggatctac
    19741 tcctgttgat tttgcctatc tcattcacac ggaagtagga aacaagtgcg tgggagctaa
    19801 agtgaatagg cagatggttc ccttaagcta tgagctaaaa agtggcgata tagtagaaat
    19861 cattacctct cgttcttctt ctggacctag cgctgattgg ttgaaaattg ctaaaactcc
    19921 aggagctcgc aacaaaattc gggctttttt gcgcaagaaa aatcaggacc tcaaccggga
    19981 aaaaggtaga gaagctttgg aaaaagaatt ggataagtat tctctaactc cggaagaaaa
    20041 agcgctggtg gcaagaaacc tctctcagtt tagagaagag aatcagcttc gggaagagga
    20101 agagctttat attgccattg gtgagggaag atacagcgct cgtcaagtgg cgaacaaagt
    20161 ggttccttac actattcgcc gccgattgca gaagaaaaag aaagaatccg gtgctattac
    20221 tcgggaagaa gaaagaattg tcgttcaagg agctactggt cttgcagtaa aattggctcg
    20281 ctgctgtacc cctgttccgg gagaccaaat tgtggctttt gttaccaagg gcaaaggaat
    20341 taccattcat cgggtggatt gtcctaattt gactagactt tctcaagacg gagaacgttt
    20401 cttggaagca tcctggggag aagaggaggg aattaaatat catactggag tggtaataga
    20461 agcgttagac cgtcctaaac ttctggctga tatttctaat gctatatcta actttcaggt
    20521 agagataaaa gcaatacggg ctaatactat aggtaatgaa gctcgaatat atctcattct
    20581 agaagttaaa gaccggcaag agctggatgg tgttttccgt gaggtgaaaa agatagccaa
    20641 tgtaagaggt gtccgacgag gaggagcgtt tgccttaaga tgaaagcagt ggttcagagg
    20701 gtaaagagag cccgggttaa agtagaagaa aaaatagtgg gagaaattga aaagggtctt
    20761 ctggtttttt taggagttca tagggatgat ggggaaaaag acgctcaatt tctgataaac
    20821 aaagttccct atctgcgcat ttttgaagat ggagaaggga agatgaaccg gaatttaaaa
    20881 gaagtgggag gagagattct tctcatttct caatttaccc tttacggtga ttgtcggcga
    20941 ggactgcgac ccagttttga ccaggctgct cctcccgagg aagcagaaaa gtggtatgag
    21001 gaagtaggga aagggtggga gaaagagggg ataaaggtag agcgaggagt atttggagct
    21061 tttatggaag tagaaattca caaccaaggt ccggttacta taattttgga tagcaaagaa
    21121 aggagaaata aatgacctta aagctcgagc attttgttct gggagattta ttcaccaact
    21181 gttacctggt tcatgataat ttggaggctt tgctcatcga ccctgctgct ccttcccagg
    21241 aaataaaaga ggaaatagaa gcgcggaatc tcaacttgca atatataatc aacacccatg
    21301 gtcatattga ccacattgga ggtaatgtct tttttaagga acattttcct caggctcgat
    21361 tagtaatagg ggaaagagat ttagtttatc taatcaaccc ggacctaaat ttatctcgag
    21421 atttttcctc tccttttgtt tctcctcttc ctgactgggt ggtaagagaa aacaatttat
    21481 ttctgaacac ttcctgggaa aggctttctt ttctccttac tcctggtcac acccccggaa
    21541 gtgtgtgtct tttctttccg gtggaaaact ggctttttag cggtgatact ctttttgccg
    21601 gatcggtagg aaggactgac cttcccgggg gttcatttcg cgacctcatt tcttccttag
    21661 aggagatttt taatcgcttt cccgattctg ctttcgtgtt tcctggccat ggtcctttta
    21721 ctagtttagc tcgggagaaa aaagagaatt tttattatct tcaatattta gctccagact
    21781 aaattccctt tatgaggtct aagtattttt gaaccagctt ttttgctttt tcctggtctt
    21841 tggattcagc aaaaactctg accaatggct ctgttcctga gaaacggatt aataaccagc
    21901 tcccgtcttc aaaaaccact ttatagccgt ctatggtgat aatctctttc tgattttttt
    21961 cttcagttat tgctttctct acttctttta agattctatc tcttttttga ggatctattt
    22021 ccagtttggt ttttacctgg taataaggag gcaactctct gaggagctca cttaaagctt
    22081 tccctttttt ggccattaag tgaagaattt gggctgctgc catagctcca tctcttcccc
    22141 acacaaaatc ggggaaaatc atccctccat tttcctcgca accaaatatc cctccatgct
    22201 cttgcagagc tttagctact actaaatctc ctacttcagt tataattact tctcgattgg
    22261 tttcccgggc aatttcttgc aaaatatgag tggtggctac agtggtggcg atggggccag
    22321 gagtttcccg gtgagctaat tctcgagcta ccaaacttac actcaagtcg ccaggaacaa
    22381 actctccatt ttctttaatt actattagcc ggtctccgtc tccatcctgg gcaaaaccca
    22441 cagcaaaagg agaatttttt ataagggcga ttaaatcctg taaattttcg gggacaggct
    22501 caggatttct tccggggaag ttgccatcag gatgagaatt gagactcacc acctggcatc
    22561 ctaattcact caaaagataa ggggtgatga gggagggagc tccgttagca caatctaatg
    22621 ccactcggaa tcgtctttct tttatggctt gagcatctac tttttggatt atagcttcaa
    22681 tataatccca gcggatatct ctttttttgg tttctgattg gattttcatt ccggagacac
    22741 gagtgaattg ttctccaaag tagatttttt ccacttcttc atctttttct ctatctaagc
    22801 cttttccagt tttttccatg aattttatac cattccactg agggggattg tgactggcgg
    22861 taatcatagc tccccattga ttccattgtc ggcaggccca ctgcagggca ggagtagcag
    22921 tgactcccag ttcagttact gagcatccgg cagaaagtaa cccggaaatc acggcataat
    22981 aaagcatttc gctgtgcttt cgagcgtcgc gccccactac cacttcctct ccagagataa
    23041 aagttccaaa actgagagct aatttagtgg ccatctcggg agtgagaact tcgttagcta
    23101 ctcttctgat gccaaaagtt ccaaatagtc tcctcatctt cttcctcctc tcattagaat
    23161 gagtattaaa tcttctttcc cttaattatt ttaactcaaa aaagaagtga atggtacaaa
    23221 tagcttttta actttctgag catcttttca gttataatag aggttgctca atttttaaaa
    23281 atacaatgaa aggatggaag ttatgaaaag gatactttgc tttcttacct tgattttgct
    23341 tttgtttttt tcttcagggg cggaagctta tgagattctt ctctatggta accttaccca
    23401 gcctcttcaa gaagatggag tagctttaca gattgacctg ggaggacacc agttaaaaga
    23461 agaggaatgg aagaatattc tagatagctc cgagaacact gtacttttgc cttctcttgg
    23521 ggatttagaa aagggaatct cattcctgga agagaataaa gggaaaatct taggaattgt
    23581 ggtttcggac tcctttgctg aagaacaatc aattttcgca ccctttctct cttttactca
    23641 taattatcac cgggttttaa tttttaattt tttgcaggat aaccccgaag ttccctggga
    23701 taaatatctt gaagagacag aagaagtaga tttaataata gtggtgggga cggagaataa
    23761 tcttccctct cttcgggaga aattttctgt gctgggagaa aaagtgcagt tcatagcaag
    23821 aggttcttct cttgattttc aagaaaaact ttcggtggaa gagaaacctg tacttttctt
    23881 tttctattcc tctcgttgcc ctacctgccg gcgcttaaaa agcgaggtgg ttcctcctat
    23941 agtggataaa tataaggata aaataaaagt ggtctatctg gattatcttt tttctgataa
    24001 ctatgagaga atggtggaat gggaggaaca ctggcaggta gagaataaga cctcggtaga
    24061 ggtattttct gacgctggtt ttgtagcaga agaagatgaa aaggaatttg ttgctcagtt
    24121 agaagaactt atccaaaaaa ctatagaatc agggaaaaag aaggtaagtt ctccccagga
    24181 aggggagaac ttaatcttcc gtcgattcca aggatttacc ccctgggtaa taataggagc
    24241 aggtttttta gatggactta acccctgtgc ttttgctact atagttttta tggtgaattt
    24301 acttctacta ttgggacatt ctcgccgtca catcctggcg attggtttaa cctatagtat
    24361 agcggttttt attacttatc tccttttagg cttgggtctt tttcaattct ggcaggtttt
    24421 atcttcttat cagactgctt ctcgaatact ttatttaatt atggctttat tgcttctagt
    24481 ttttgctgct ttgagcatta aagacgccat tcagtataaa agggaaggaa aagaaactgg
    24541 tatgactttg gggcttcctg aaagttggcg agttcgtatt aaccagtatc taaaaaatag
    24601 ttttaccgag aaaaacttgg tagtggctgc tattttaagt ggttttgtta tttctctctt
    24661 agaggcagga tgtaccgggc aggtgtatct tcccaccatt atgtatattg ctcgagaaag
    24721 tccttaccgc tggcaagcat taggatacct tttgctttac aactcttttt ttattcttcc
    24781 tcttcttgtt gtatttttaa ccatattttg gggaagtcag tctaaggctt tagttaattt
    24841 tgcgagaaaa aacatcgtct tttctaagat tgctttagct atccttttta tctttttaag
    24901 cctgcttctt cttgaagggg ctttatttta aagctataga ggtaagctaa taatattata
    24961 gttctatgct caaagcttgt gtaagaggag gactataagg gataggagtg tccattgtaa
    25021 ttcctttgaa aggtagcctg ctgccttttc tccctcttcc attatgcctg aaatttttta
    25081 tcaggcatct acgtttttgc tttccctctt ttttgttttt ctattgagaa tcatagaggg
    25141 taacccccct atagtttaga ttcctgatag gaacattcag gaatgataaa ggtgggggct
    25201 gataaatata tttaaaacaa tgggcttgag gaatcaaggg aaaagagtca aaagagagaa
    25261 aaatcagagt tatgaggtgt gctgcctctc ataacccccc caaaaaagtg aaattgaaaa
    25321 agatggaaaa gattcaaaca ataacgctct ggaaaagtgt cccgatgcgt ggggcgtttt
    25381 ggattattac agaagaatat aatacaaccc gtacaataga ggagaaagag aagaggagga
    25441 ataaactatt tttaagcttt aaaatttttc ctattgacaa acttaaaaag agatgatatt
    25501 ctatatttgc catcggttct gaggagctaa gccggaggat ggaaaaaaga gcttctgttt
    25561 gaaagattag aagctcaaag ctttaccaag tggaagtcca tcctgaagtt tctcactcaa
    25621 aaggtgagag aagaaaaggc taaagcttgt tggagccgat ggttttttat ttggccttat
    25681 aagttgaact atgagaaaag agatagtatc atcgtgaatt acattttctc ccttggtgcg
    25741 gtctatttta gctattgtgg ataaaaaagg agattgcacg agaaggttat gcgagactat
    25801 caataacccc ggctattata taagaagttg aggtttgtaa taacgacaac ctactgtgta
    25861 aacttaaaaa ataaaataaa tgaaaaaact aaactataat gttattggaa cacactccag
    25921 cgttgagaga cactttctca acgctggcga ttagcataca ccgcttatag tataaaagaa
    25981 acggaacact gagcagaggt tatggatgtt tgtatgcttg actatagttc ttgtgtctgt
    26041 gttgtaaagt tccctgattc ctcacctatt tcttcttcat ttgacaatct gggtgatagg
    26101 agttattcta taatcgttag cttggttgaa ttgggttact gagttagcta atttgtgatc
    26161 gggaaggagg tggaaataag tgaagtcaac tcgcaaatat gtattattgg gattgggagt
    26221 agttctactc cttgccttgc tttctggttg tgaagtaacg gttggacctg agccaccggt
    26281 ttatgggagt ataagaatat gtgttcgtga gggatcagtg atttctggct gggtttatat
    26341 tgatggtaaa gatatggacg agcatgttga tgggtggtat ggaccttatt gcacagattg
    26401 gaatcgcatg actttggaca aagagcatct tttggaaata cgaggaagta gtaggacttg
    26461 gcggacttgg tttgctccca ctcgtgaagg acaaaccttt attcttgaca ctcatgatgg
    26521 ggaacatcta attatttatt aagtattttc aaatcagtta ttctcttctt gaggtatcct
    26581 ctaacactaa gttgtagccc cgctgaggag cccgcagctc cacaaggcgg ggtgtttctt
    26641 tttggtagcg aaggtaggta atttctcctc ctaaagtgaa ccgaaaagtt tcttcttctt
    26701 cactttcggc aattttctgt atttcccctc tccataccat tcctgaaata gggaccaaca
    26761 ccgatatagc tttttcttca taagataatt ttcctattaa atagggtatt tcttcgctgt
    26821 cgaaagtagg agagggaata gaaatagtag cttctttagt tccttcagga gaagtaattt
    26881 taatttgggc ttttccttct tggtaggtag tttctatcac tatttctcct cccgggattc
    26941 tgacccactt ttgagaatga agaggctcat aagtatccgg tgatatggtg atttgagcat
    27001 cttgttctct tttcccttcc acttcgataa aagactggat ttgccaggca ccattttctt
    27061 cttctacttc ataaatcatt tttcccactt cttgttctcg tatgattatt ttatagttaa
    27121 aggtctcttt cgagagtaga gaggaagtgg taataaaaat tacccccaga attataaatg
    27181 ttctcattat taataaagat aacctcttgt ttaaaacctt tttcatttta tactcccttc
    27241 ttgggaagcc tctatgggag gcacttcgtc ttcctggata atttggtaaa gtcggcgacg
    27301 ataaagatgc cacaataggc caaaccctat gattaaggta agaggaagaa ggctgatgag
    27361 ataaactgaa gctaaaaaag aaagagaggg aacaaactgg attaaaactt gcgctaaacc
    27421 cagcagtcct ttaggtagga aggagaggag ggaataggag accaagttat aaaaggtcca
    27481 tccccggggg gagagaaaag ccaaacccag gagtcctgca gatatgactt caatttgggc
    27541 ttccatgatt ttctgcaaaa gataaaaaaa ggaagggaaa ttagcaaatt ggctatatcc
    27601 ataaaatgta gagagggagt ggaaatagac cagcagcgta tctcgcaatg ctttccccaa
    27661 tcccaaacct aaccccaaaa ggagaaagag aaaaaacttt tcctcgatag tttcccttgt
    27721 ttcttgattg gctttcatta atagaaaaag gagcaaagcc cattctataa gtattaaaac
    27781 taaagaaata aaaatagcgg acggtagtcc agctaaataa tcaaagagta ttttttccag
    27841 ccatcgataa ggaattacca gaggtaaaat taaagataag agataatata aaactaattt
    27901 ttttctggga aatttagttt ttctaaaaaa tagggctaca aaaagcccaa aggctattaa
    27961 tattgtaatt acgatgtacc ataagtaatt caattttcca cccctctttt ttcgcaaaat
    28021 tcttctatag gacactcaaa acagcgagga cggcggggaa ggcagatgtt ttgtccaaat
    28081 aaaactaaaa gaggattgat aacttcccag taagtaagag gtagcttttt tcgtaactcc
    28141 ttttcagtct cttccggggt tttagtttct acatatcccc agcgattcac aatccgatga
    28201 atatgagtgt ccacacttat tgctggcttg tgaaaaccta tggttaacac taaattagcg
    28261 gttttacgcc ccactccagg cagttttaat aattcttcta aatcatcggg gacttcccct
    28321 tgataacgag tgaggagaat ttcagctatt ttccgcaaag ttttagcctt tcggcgataa
    28381 aatcccaccg gatatattat ttgagataat tcttcttccg gtatttttaa gatatcttcg
    28441 ggagtgggag ccactgctcg gagcctctgg gtagcttctc tagtgacctg gtctttagtg
    28501 cggtgactca agatacagct cgtgaggacc aaaaaagggt cactgcctaa actattaacc
    28561 gtattatctt gccattgtcc ctttatttct tgtaaaatag cgaaagcttt acctaaattt
    28621 tcatcagtca taggataata ataaacgcag gagggtcaaa aatgcaagtt aaaataattg
    28681 ttctactgat ttatgtggta ggtatgcttc tcataggcta tttcagtatg aaaaagacta
    28741 aaacaatagg agacttcttt ttagctaacc gtagcttagg tccttgggtt tctgcttttg
    28801 cttatggctc aagctatttc agtgctgttc tttttatcgg atatgcggga aacattggct
    28861 gggggtttgg cctttccagt ctgtggattg tggtggggaa taccttgata ggttctcttt
    28921 tagcctggtt ggttctggct cgacgcaccc gattagttac tgaggaaatg aaagttatga
    28981 ctatgcctga gtttctacaa gttcgttatg gcagtaattt tttgaaggct ttttctgctc
    29041 ttttaatttt tatattttta gttccttatt ctgcctctgt ttatatgggt ttaagttacc
    29101 tttttgatgt ggtaatgggg attccttatg tttatgctct tttgttgata gcagggctaa
    29161 ccggagttta tctggtaatg ggaggttatc ttgcagtaaa tctcactgat tttttccagg
    29221 gtagtataat gctgggagga gcgatttttc tggtggttta tctcgtaggt cgtccagaag
    29281 tggggggatg gagccagatt gtaactcgct taagggaggt aaatccgcaa ttagtaggag
    29341 tagttggtcc tccaggattt tggcctcttt ttggtttggt ggttttgacc agtttaggtc
    29401 cttggggctt acctcagatg gtgcaaaaat tttatgcttt gaaaaacgaa aaaatagttt
    29461 acactgctac catagtggct actattttcg cttttatttg cacttttagt gcttatttat
    29521 ctggttctct aactcatcta ttttttgatc aactccccca gattaatgga gagcctaacc
    29581 ctgagctttt aatgcctcat ttaatttctc aggttatgcc ccctctcttt tccttaattt
    29641 ttcttcttct ggtattctct gcttctatgt ctactctttc atctttggtt ttggtttctt
    29701 cttcggctat taccattgat ttattgccgg ctcgttttcg ggataagtct cttcctctta
    29761 tgcggatttt atgtgggata ttcatttttt tctctctttt gattgctttt taccgagtta
    29821 cttttatcgt aaacttaatg tccatttcct ggggagcagt ggcaggaagt tttttagccc
    29881 cctatcttta tggtttatat tggaagggag ctaatcggtg gggagctatc tccagcatgc
    29941 ttgcaggtct ggcggtttct ttgggctttt cctggtatta tggctttaac tcctcgatgg
    30001 cacctttggg gggttcttta gctatgctta ttcctctcgt ggtgttacct atagtttcta
    30061 tcctgacaag aggtgaagaa agtgttagct tgcgtattcg agaaaaagga acagtgggca
    30121 atacgggaga ttgaagaacc tcgtattaat ccggatgagg ttctggtgcg ggtaaaagca
    30181 gcgggaatct gcgggacaga cgtccatatt tttcgggctg aatatttttc cgattttccc
    30241 ctagtagcag gacatgaatt ttctggagaa gtagtgaaag tgggaagcga agttactcaa
    30301 tttaaacctg gagaccgagt taccgctgac cccaatattt tttgtgaccg ttgttatttt
    30361 tgcaaaataa acaaaaacaa tcattgtctc aacttgcaag cagtaggagt aacgagaaac
    30421 ggtgcttttg ctgaattagt agctgtcccg gagaaatgtg tttttcatct tccttctaat
    30481 ctcagttttt cagaaggagc gctcaccgag cctctttcct gtgtggtata tggagtaaag
    30541 aggagcaata ttcaaccggg agaaaaggtg ctcatctttg gagcaggagc tattgggctt
    30601 ttacttttat ctttgtttaa ggtaagtgga gcctctcaaa tagcggtagt ggatgtaagc
    30661 gagaagaagt tgaatttagc tcaaaagtta ggagcacagg aggtagtttt agctgatggt
    30721 agagaaggga aaagattaaa agagattgcc cctcgggggt ttgagatagt ggtagatgca
    30781 acaggaatcc ctcaagttat ggaaaaagag ctggagtttg tcgaatcaga tggcactttt
    30841 ttaatttttg gagttgctcc tcgaaattcg gtgatagcca ttgaccctta tgagattttt
    30901 cagagagact tacgtattat tggctctttt gccgttaaaa agactatgca gtatgctttg
    30961 aatcttttag aaagtggcaa agtgaaagta aaagacttga tttctgctac ttaccctctg
    31021 gctgattttg gtaaagctat ggatgatgtt ttaaacaata aagaccatct gaaagtgcaa
    31081 atagtttttt aaggtaagga aagagaatta aaaataccaa acactggatt tttcctttgc
    31141 agaatgcccg taaaagaaat aactggtctt tgtactcaat atattttcag ggagtagagc
    31201 tctgccctca gaggaagaga aaacccgtgt atctattgaa aaaaaattta atggaaggag
    31261 tggtaagaag ctaatttgga aacatttaat tttcaatggc acattaccaa tcgttgtaac
    31321 cttcgttgtc atcattgcta ccaggaaaaa tttcaggatg aggtagaaga ggggcatctt
    31381 cttttagttg ctcaccgact gatggaagag ctggaaaaaa gagaccttca taccgtcata
    31441 aatattactg gaggagagcc gcttctttta ggggaagacc ttttctctct ccttaactat
    31501 cttaactctc atccccgggt gggggagctt atgattatta ctaatggtct ccttttagaa
    31561 aaagacctgc tttttcgctt ggaggaatac cagaagctaa ccactttaaa gatatcgtta
    31621 gaaggggcaa attcttttac caatgattcc attagaggac gaggagtatt tgataaagta
    31681 gtaggcaaaa taaaattgac tgaaaaagtt tcctctttag aagtagtact catgttcact
    31741 ttgagtaaaa gaaatgtaaa agagttctct tctatgttga cgcttgctaa aaatttagga
    31801 gtggaaggag tgatggtaga gcgctttgtt ccccagggta gagggaaaga aatgagggaa
    31861 gcaactctct ctcgggaaga gtggaggagt tttctcgagg aactcataaa tatttgtgag
    31921 ctggagtgca ctccttatga ccttcttcct tatcgggctt tctggataaa aggggaagat
    31981 gaggcagagg tactgggggc tctgtgcaat ttaagagagg cgctctgtct tatgcctgag
    32041 gggactattt ttccctgtcg ccgttttcct tatcctctgg gtaatatttt agaagatggt
    32101 ttggggaaag ctattggctc tcctttttta gaaactttga aagataggaa acaattgaag
    32161 gggcgatgtg gccgctgcca ggtagaaggt tgctatggtt gccgggcgtt gggttacgct
    32221 ctatttaatg acccttatta cgaagatgtg cagtgtttct gggaggaaac tatgccttaa
    32281 tttctactat atctttatcc tgtaaaatat aatcaggggt gaccagttgc ccctcaaatt
    32341 tagctgagcc ccatactcga gcccctttta aggtatggac taaatcttta tgtatagcac
    32401 aggctaaatc ttttactgtt tctccttttt tcagtataaa agggcgagat aagtcaggag
    32461 gttttccggg gggtttactg tatattcgta taattgcagc cagatagaaa atatctcgtt
    32521 ttagattttc tttttcttcc gcagacatgg taagaagaga aattccttgg atagtaaacc
    32581 ttgcctcata taattctttg aaaatggaaa aagtttcttg ctccagcggt gtttctatct
    32641 tatttactaa gattagcgac ctttcttttt ctaactcaaa cttactttct tccaaatctt
    32701 caaggagacc ctccatttcc tccagcactc tttcgctttt accatccatt accactatca
    32761 ctccatccgc tcttcggaga gcgcttagag cattaccttc tattttccct tctaaccagg
    32821 ggggaaaatc aatgagctga atctgaatat cctggtattc catcattcct ggaagagggg
    32881 agtgagtggt aaaagggtag tcggcgattt ccggatgggc acgagttaaa agagagagga
    32941 gagtagactt tccgctgttg ggagtaccaa ttatggcaat ttgagcagcg cctacttttt
    33001 ctataagata ggggtcaaat agttttcctt tgccctttct ttctccttga gatttacgca
    33061 gtttagatat tttttgcttg atttctgcct gtagcttttc cgttccttta tgtttgggta
    33121 ttactgctaa catttcttcc agagctttta gtttttcttc cgatgttctg gcacttcgat
    33181 aacgggcttc tgcttctaaa tattgaggag ttagattggc tggcaacttc tcttactctc
    33241 cttgagattc tcgagttagg gggacaaatc ttactgctcc taaatctcga gtggtgatac
    33301 cctcttcagt tttttctacc acccatagac tttgtatatt ccagggaggg cttaccggaa
    33361 taactatttt cccgccctct ttcagctgag caagaagagg agggggaaga tggtctactg
    33421 cacaagttac tattattcgg tcaaaaggag cttcttcttc ccagcctaag tatccatctc
    33481 cctgttttac tgttatgtta tcgtagccta actcttttaa ctttttctct gctgtcttgg
    33541 ctaaatcttt aattatttct atggtataaa cctggcatcc cattttagat agaatggcag
    33601 cctggtatcc tgaacctgtt cctacttcca gcactttaca tccgggcaca atttgtaaat
    33661 tttcggtcat caaagcgaca acataaggct gagaaatagt ctgtccatat ccaataggga
    33721 ggggagtgtc gtcataagct tgattgtgca agttttcagg aacaaactcg tgacggggca
    33781 cctcttgcat agttttgaga accagcgggt ttttgactcc tctttttttt atttgttgct
    33841 ctaccatttt gatacactta tcttcccacc cttccataga aaaagagtta cttttcggac
    33901 ttctgctctg gaggtagaga ataaaagaaa tacccactat taaaattact atcacccaga
    33961 ggcacttttt catttttatt actcctgata ttatagttaa ccacaaaatc cattacaaat
    34021 actataaagg ggagcacccc tttataatcc cccaaaagac agacagccta cggcgtaata
    34081 ctaaaaagca aattagataa gaaagattaa aactataatg ctggagataa aatactccag
    34141 cataacaacc ttgagttttt gactttcggt gtaatttact ttttaaaagt tttaatattt
    34201 taaccagttt tgcgggatac tttaaaaatt tctgatttaa ttttaacttt aattaaggaa
    34261 aatagtaaac aaaaatatgt ttttagtgat aa
//
LOCUS       DAOSVE010000007        32236 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_958_length_32236_cov_79.739598, whole genome shotgun sequence.
ACCESSION   DAOSVE010000007 DAOSVE010000000
VERSION     DAOSVE010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 32236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32236
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_958_length_32236_cov_79.739598"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2516
                     /locus_tag="PK016_01245"
     CDS             <1..2516
                     /locus_tag="PK016_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF499 domain-containing protein"
                     /protein_id="HXL01739.1"
                     /translation="TQGLKNLLSNVLSRLSGGKGDPVIQIQTPFGGGKTHALLALYHI
                     VKNYDQVKHLSLLSEIQSPILKEAKVVVFVGTHADPIGGRTIWGEIAHQLGVYEKVKE
                     HDEKRRSPGKGVLYEVLGEEPVLILVDELVEYAVKAKDFADQISVFSQELTEAVKSKP
                     NACLVSTLPSSAPYGEAGERALNELQRIYGRVETVYTPVEGIEIYEVIRKRLFEDFGD
                     EKTRKEVAQSYFELYQKLGPDVPSEVREIEYRDRIEHAYPFHPELVDILYERWGSYST
                     FQRTRGALRLLAEVVADLYKRQIPSPLIQSSLVNLENQAIRREFIKHIGNEYDSVIAA
                     DIAGKNAKAPKIDREMGSEYEKYGIATGIATSVFLYSFSGAEKKETTLPRIRVALLRE
                     GIPSTIVSDAIGKLEEELWYFHSEGKQYAFRNQPNLNRVIMDREETISESRVREKLQD
                     SIQRYAGNALEVYLWPESASDIPDNKNLKMAILAPEFSYNPGLSAAEQSGKVNQLVSE
                     LLEKAGTGFRVYKNTLFILAMDDAQYSTLYKSLKRLLAISEVQNDRGLLESLTKESQN
                     ELKKKLNDAEKEIPFRILNAYRHLALLEEKGWAWKDLGIPIVGTSVTISERVKQHLKD
                     QERILSRLTPKYIIDRTLAGDENEKGARDIYELFLKTPGMPILEDERVLLDALREGVR
                     SGFLGIREGQKVYYSEDVSPTMDSIVLRSEVAKEIKEAEKEKEEKGKEETRESGGWKV
                     QERYEEGKEEGGEEVREEPRKSGAVAKLTLRAKIPWDKLSSVVGGVVRPLKEKGLPPE
                     ITIEIKAESQEGFDKITLESKVKETLKQIGAEIEEWREE"
     misc_binding    2738..2950
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2998..4659
                     /gene="ilvD"
                     /locus_tag="PK016_01250"
     CDS             2998..4659
                     /gene="ilvD"
                     /locus_tag="PK016_01250"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HXL01740.1"
                     /translation="MRSEIVKEGPDRCPHRSLFFATGLTRESLNRPLIGIASSFSDLV
                     PGHIHMRELERFIERGIEAGGGTPFVFGLPAICDGIAMGHEGMRFSLPSRDVIADGVE
                     AVAQGHALDGLVLLTNCDKITPGMLLAAARLNLPTIVVTGGPMLSGVYRGRRLSFVRD
                     TFEAAGKYHKGEISLEELEALEMEACPGAGSCQGLYTANTMACLTETMGLSLPGCATA
                     LAISAQKRRIAYESGKRIVELVRENISARQILNEKSLRNAIKMDLSLGGSTNTVLHLL
                     ALAQELQTSSISLTTFDELSRKIPHLVMLRPAGELFLEDLHFAGGIPAVEKTLGELLE
                     DNLTVLGKTVREIQKEVEEISIDREVIRTLDNPHSPEGGIAVLYGSLAPDGAVVKQSA
                     VEPEMMVFRGKAICFNSEEEAMEAITNQKVSPGSVVVIRFEGPKGGPGMREMLAPTSA
                     VVGMGLSKTVALITDGRFSGGTRGPCIGHIAPEAFEGGPIALIREGDEILIDIPHRQI
                     ELLVDKEELERRKKGFTPPLPKYKTGLLGRYIRLARPTSQGASME"
     gene            4675..6339
                     /gene="ilvB"
                     /locus_tag="PK016_01255"
     CDS             4675..6339
                     /gene="ilvB"
                     /locus_tag="PK016_01255"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HXL01741.1"
                     /translation="MKMRGAQILIELLKREGVEVIFGYPGGAVIDIYDALMDDKDITH
                     ILVRHEQGAAHAADGYARTTGKVGVCMATSGPGATNLVTGLANAYMDSVPLVAITGQV
                     ATSLIGKDAFQEADTTGITMPITKHNFLVTSLEELPTVVRQAFYIASTGRPGPVVIDL
                     PKDIQQQEGEFVFPEKVKIPGYRPTYRGHINQIKAAAQAIQEAEKPVLIAGGGIISSG
                     ASEMLLKLAEKADIPVTHTLMGKGAFPESHPLSLGMLGMHGTYWANKAVMNCDLLIAI
                     GMRFDDRVTGNLATFAPRAQVIHIDIDPAEIGKNVKPSIPIVGDAKMVLSELEKYVEE
                     KKHPIWREKIEEWKKEYTPQVSKGLSPRQVIEKIYELTCGDNPIVVTDVGQHQMWAAQ
                     IYRCESPRQWASSGGLGTMGYGLPASVGAQVGNPDRLVVLISGDGSIFMKIQELVTAV
                     NYCLPIKIFVINNSYLGMVRQWQGCLYEGRYSCTKLQNPDLVNLVQAFGGEGFKVEKE
                     EELEEIISKALEVKNKPVLVDCRVVKEENVLPFVPPGKSLEEMILE"
     gene            6356..6850
                     /gene="ilvN"
                     /locus_tag="PK016_01260"
     CDS             6356..6850
                     /gene="ilvN"
                     /locus_tag="PK016_01260"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014404822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HXL01742.1"
                     /translation="MEHIISVLVENRPRVLARVSSLFARRGYNIESLAVGPTHDPTIS
                     RITLVVEGNTDTLEQITKQLYKLIDTIKVGDFTQTNYVDRELALIKVNAPSEVRGEIV
                     QTVEIFRARVVDVEEKSLIVEVTGTPDKIDALEQLLRKYGIIEMTRTGRIALARGSQS
                     LQEK"
     gene            6878..7870
                     /gene="ilvC"
                     /locus_tag="PK016_01265"
     CDS             6878..7870
                     /gene="ilvC"
                     /locus_tag="PK016_01265"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HXL01743.1"
                     /translation="MSKVYHDEDANLQVLQGKTIAIIGFGSQGSAQAQNLRDSGLNVL
                     VSDLPGTPGFEKARHAGFSPVSASEAAQKGDVIQMLAPDQYQASIYKESVAQYLKAGK
                     VLMFSHGFNIHYHQIIPPQDVDVVMVAPKGPGDLVRRMYQEGKGVPSLIAVYQNASGK
                     AKDIALAYARGIGATRAGVLETTFQEETETDLFGEQAVLCGGLTALIKAGFETLVEAG
                     YQPEAAYFECLHEMKLIVDLVYEGGLTNMRQVVSDTAEYGDLTRGPRVINDQVKEEMK
                     KILGEIQDGSFAREWILENQANRPVYYALRNKDIQHPIEKVGKTLRDMMPWLKK"
     gene            7883..9427
                     /locus_tag="PK016_01270"
     CDS             7883..9427
                     /locus_tag="PK016_01270"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HXL01744.1"
                     /translation="MRNIRIFDTTLRDGEQSPGVSLNPQEKLEIAKQLSQLNVDVIEA
                     GFPIASPGDAEGVKLIAQQVKGPAICALARCKEKDIEVAGEAVKYAERPVIHVFLATS
                     DIHLQHKLHLTREEALEEAVWAVKKARTYTNEVEFSAEDATRTDWEYLSQIYAAVIEA
                     GATTLNIPDTVGYTIPEEMKELILYLLEHTPGMDKVGVSVHCHNDLGLATANSLAAIS
                     AGATQIECTINGIGERAGNAALEEIAMALYVRQDRFQAQTSLNLQEIYRTSRLVSRLT
                     GMLVQPNKAIVGQNAFAHEAGIHQDGVLKDKLTYEIMDAQLIGREERVLVLGKHSGRH
                     AFAKKLEDLGYQLSEEELNQAFQRFKQLADQKKEITEADLKAIVSEEVSRPEEMYRLD
                     FVHVCSGNNILPTATVRLIKEDGEILEGVSTGVGPVDAAYKAISKMLGISPNLVNYTL
                     QSITGGTEALGEVIVRIIGEDQEYVGRGSNQDVVVASVLAYLAAINRMAQKRKKINPQ
                     KDYKEN"
     gene            9429..10688
                     /gene="leuC"
                     /locus_tag="PK016_01275"
     CDS             9429..10688
                     /gene="leuC"
                     /locus_tag="PK016_01275"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HXL01745.1"
                     /translation="MGMTITEKIIAAHAQKEKVAPGELVQVKVDLALANDITAPLGIK
                     ELEKQGIDRIFDPDRVVLVADHNTPCKDIASAQNVKLMRDFARKYGISHFFEGGKVGI
                     EHVLLPEQGLIVPGDLVIGADSHTCTYGALGAFSTGMGSTDIAASWVLGETWLRVPES
                     ILFLYEGKRKPFVTGKDLILFTIGKIGVDGARYCAMEFRGSVISELSLDERFTMANMA
                     VEAGAKNGIMEADGKVLSYISERTSRPCVVYQSDSDAQWREVIEIDVSPLEPQVAFPH
                     LPSNTHSVYSIPKIEIDQVVIGSCTNGRMEDLRMAAQVLKGREVNPGVRLLIFPGTPA
                     VLKQAEKEGLLRIFLEAGGIIAPPSCGPCLGGHLGVLAEGEKCVSTTNRNFLGRMGHI
                     KSEVYLANPYVAASSAIAGYIAAPDQI"
     gene            10703..11197
                     /locus_tag="PK016_01280"
     CDS             10703..11197
                     /locus_tag="PK016_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HXL01746.1"
                     /translation="MIISGKALKYGDNIDTDVIIPACYLTSTDPAELAQHCLEGLDPT
                     FSEKASSAILVAGKNFGCGSSREHAPLAIKASGAKAVIAASFARIFYRNAINIGLPIL
                     ESEEAAEKIKEGDQLEIDLDRGEIKDLTSGDTFKVNPFPPFLQEIIERGGLMNLVKER
                     VKSS"
     gene            complement(11335..12060)
                     /locus_tag="PK016_01285"
     CDS             complement(11335..12060)
                     /locus_tag="PK016_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL01747.1"
                     /translation="MKVARAEIKKAFHSPRFYGGILISWALAGLVVAQVWRDPLSFYL
                     RKITLPFSFLYPLALFGPVGGIWGGTENIAEERMEGTLSTALLRPLSRWEWYWGKVEF
                     TLFYNLLMVFTPLLGMALLEKIMWGGGFSFRLLGGGLVVTLYISSFSALSYLFSSLFY
                     RPWGGPLLSLGVGAFSYIIEHLGEGERIQIYLLAHHLSSFNVFFLPQIDWHQAFISFL
                     VLGSYTGGGFLLGALIFLKQEEK"
     gene            complement(12057..12830)
                     /locus_tag="PK016_01290"
     CDS             complement(12057..12830)
                     /locus_tag="PK016_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL01748.1"
                     /translation="MKLFFSGREKEKKILVNIPMEDDIILQLKNVEKKARKRAILEEV
                     SFEVRKGEIFGLLGGNGAGKSTLIRILFGLVSPSAGCFYVGNYRCPPCLPESKALIGG
                     CLDNTSFYGNLTGIENITLFASLSSYYPRVKEIEFFASLLGLESSLLYKKVSTYSQGE
                     RKKISLLHSLFPLPRILVWDEPWAGLDPQGAKKIQEFILTLNTEEGVTFFLSSPLPQE
                     MEKVAQRIAILHRGKIKWNDYLTPQVSLEEIYWEIIEGK"
     gene            complement(12763..13413)
                     /locus_tag="PK016_01295"
     CDS             complement(12763..13413)
                     /locus_tag="PK016_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01749.1"
                     /translation="MEKKRIWWWNNSCFRSFLLGVVILLFLSRTGGALPTFVIEGGES
                     FPFLLPMVESAWEEAQLCFQRVTGYSPHSSFRIVVLSSETSKALPSWVAAFSRGNTIY
                     LRFPLLLLERGILSSTLAHEFLHIFVGSYNWQLPFWFEEGLASFFFPASGEYFQEGTG
                     EFYRECRESFQIFKEKIGEENLPSFFSTVQEKGFDEAFFLWTGEREENSGKYTHGR"
     gene            complement(13382..17800)
                     /locus_tag="PK016_01300"
     CDS             complement(13382..17800)
                     /locus_tag="PK016_01300"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HXL01750.1"
                     /translation="MKKIKILFWWVAIFLLFLGGKVLAQEEIYVSVPAHIYPGEIIKI
                     NISGRETKGAQCSLYRINDPEQYIQSVETKSFFQVDSWQLHRPLEEEEEFQNTKYSFF
                     QAVRKLASRVLPTPVKDFLRDLFDIQGPLPRRPITITPSDFRRGTDELKTFWVDLPQE
                     KDSPFFWREIEINPLPPGVYLFTAQGGGREARVLFSVTHLSCLCRQDEKKGVILVQDL
                     KTGIPQEGAQVKIWQGKEKEEGFTDSQGLYFWEKRWEEGEEAVILVEKDKEFAFLNFY
                     PYYWEEGKLKAFIYSERPLYKPGQTVLGKAILREANTDRYIVPPPGEKVEVALVDYRD
                     NEYGKETLSSTSEGSVSFSFTLPQKIEEGGFLIRLRWRDREFYQFISIENYEKPVFTV
                     QLEPEKPVYAKGEEVHFSVKGEYYSQRPLSGGEFRYQIFRYPIGYWKEERGEEIWGEG
                     EGKLDEKGEGEIRFLPEGEGAFRYQVRCVVFDPAYRAVEKQEEVRVLMGEFYLSLQPQ
                     AYFVKAGETMTYEVEARDAEEKKVAVEKVYGEVYRLEWEKEKEKWREIPSQFLNVSLS
                     QDTGEIKVTLDRAGYYRLEVYTQDKSGNKIVASSYFWVTGEDLYYPSQELEVRWDKER
                     YQVGEKARILIIPPGEGTFTFLFSIEGPNLQEAEVKQISAPEEIVIPILPSYVMGAYV
                     SFSGFSDGKLYSESLPIPLKREKELQIKITPDREKYQPGEKGKISIQVSDTQGKPVKC
                     ELSLAIVDQAIFDLSSWWINNIYDYFYSQFYNSVYGSYSVYSSFYGRGIPFQDMKAQL
                     KAQFKGNGGGAGEVLLRKYFPDTLLWEPNLSTSEEGKVEVDFVAPHSLTRWRVEVKAH
                     REEFFGEAQEYFTTYKPFALGVELPSFLVEGDTLKSKVTLWNEVAPQEVVIEGESSPE
                     ISLQWEKEPRKISAPEEAVFPLLISAQSPGKAYLRLFAQGEKAQDALELPLEVKEKRV
                     EVEENFSSFLERGKTSFEFSCDSSSTVELTLFSDPRSVLLEEIEYMLSYPYRCSEQVA
                     SRLITFSSLPLEEDKTPELVKAIREDLYTLYNLQNYDGGWGFWGGEGDLFHTAYTLLA
                     LHQVKNRGFLVSDEAIERGLRFLEDNASRSEVFPEGSFLPLPSEFEKALSWYVLSLEK
                     KDFVPPSLPPLAELDDKTLALWGLITREEDQEKVEKELEKRAHALGSTVFWGTVNPQK
                     PWEDDRFSATTFSALFLGERKNPLGEKAYLWLWRSKKGGNFFTTLERAYFFLVSSHFL
                     KEESNSTTFRVLCNGALVKEGRGEEKIAIPGQFLQEGSNHLEIEGENLFMAELKVKSW
                     KEEAPPSQFFPVKKNYYLLIPTKGEEVFFSPQEVDIFHPQEEIVCEIEIQSPDYLEYL
                     VIEDGMPAGCELIRKDYAFSLPEEEGVHYPFVVKTLFEGQPTLFVYDLLPGKNVIRYY
                     LRIRDQGNFYVKAPLLYLMYFPEVRDYGEEKNLVVE"
     gene            complement(17813..18703)
                     /locus_tag="PK016_01305"
     CDS             complement(17813..18703)
                     /locus_tag="PK016_01305"
                     /inference="COORDINATES: protein motif:HMM:NF018387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HXL01751.1"
                     /translation="MGLGILTLIFPVKLNCPSYLHLQAGGKAKVEEIEPLTLWGFPSW
                     FQRVRVVKEDEELILVSQEGRKVIFQPQRLEGKTSLLVGVFPVWQKVEVEVFPNLEDR
                     DGDGFPDMVELNTESERDAFREFFVNVARLQISSPHSQFTERDCGGLVRFAYRESLKR
                     GDILPYTYPQVPLLGVNIFRIQPGGFSPDKIEEDFSVFASSFYLLAYNVVPLGKEEEK
                     AKAGDLLFFFQPTSFSYPYHVMIYEGEGRVIYHTGPIGEGEGVRETTLASLRRHPDKN
                     WHPAKNNPNFLGFFRWKILD"
     gene            complement(18742..19449)
                     /locus_tag="PK016_01310"
     CDS             complement(18742..19449)
                     /locus_tag="PK016_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01752.1"
                     /translation="MKKIIFIFIALGLSVLLCNSFSWAHWIIEGEGKWQEEVYQFSWE
                     KEEEKMVLLLQQGEKSILRVGVDLTKPELSLIDDLNLTFSTLEDKNGIALSFLFALST
                     ILGVKEEWLFAEKPSFFLLPEPLSLPPFGDCFPFRVNDEITGWYFKEGIHPTMGEMVG
                     GVVEILEKEGLSLPFWREIIKVNGLIVAKEENEELALRVYEAKEKDTPLELPQKGEGY
                     QKVSWVDFLSPWEAILE"
     gene            complement(19463..21115)
                     /locus_tag="PK016_01315"
     CDS             complement(19463..21115)
                     /locus_tag="PK016_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HXL01753.1"
                     /translation="MIPFGRRHIRNFRRVREIIRVFRKYGFLYLFRRSQFPEQLTLPL
                     FQKETTKRTPQENLRLAFEELGTTFIKLGQILSTRPDLLPEEYCQELRKLQDQTSPVS
                     FLEIREVVEQELKKPIEEIFAEFDPAPLASASIAQVHRAVLKDGNKAAVKVQKPGVEE
                     KVISDLEILFYLANLADRLTSFAPLTFSEMVTEFQKSLMREIDFLYEAVNARRFRENF
                     SDFEGVYIPRVFDEYTTTRVLTLELIEGFNLSQMAEKGQLQIDGPFLARRGAEAVLKM
                     IFEDGFFHADPHPGNIMVREETGELVFLDFGIVGTVDERTRENMERMLLAVLRKDTKR
                     VVDILEDEFLLSPVESPLTLRVDLHEILERYVTANLKEMNISSLVNDFFYLLRKHSLR
                     FPPHLSSLMRALMVVEGTGMMLDPHFTVVPYLEKALERTFAQRLRWERLSEKVTDYLF
                     DWQKLLANFPDKAQELSEEVSRGRIRFQMESEDLKNLNRRLDRVSNRLSLSVILGSLL
                     IGSSLIYISSPRPGAMGILGILGFGIAALLGIFLVIEMLRSR"
     gene            21383..22243
                     /locus_tag="PK016_01320"
     CDS             21383..22243
                     /locus_tag="PK016_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXL01754.1"
                     /translation="MILPFVIWLVIFRYLPLWGWITAFQNYKPGVPLFQQQWVGLKYF
                     KEMFSDPEFYLVMRNTLAMSLLGLIFGFPLPIFLALLVNEIRNESFKRTVQTISYLPH
                     FVSWVIVASIVHAMLAPDGVINYLLIQIGLIEEPILFLGEPRYFWWIVVFADLWKELG
                     WNTIIYLAAIADVNPALYEAAEIDGAGRFKKMRYITLPCIMPVVTVILILSIGNIINI
                     GYERQFLLRTPAVREVSEVIDLYALDYGIRAGRFSFGTAASMFKSVVSLILVFSANRL
                     SKRVSDYRIF"
     gene            22259..23158
                     /locus_tag="PK016_01325"
     CDS             22259..23158
                     /locus_tag="PK016_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013291096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXL01755.1"
                     /translation="MTTKSFSSRDKVFDIVNYIIMTLLAIITLYPFLYVLAVSLNDPF
                     DTIKGGIVLLPRVFTLDNYKEIFNYPLIGQATLISTLRTVIGTGTSVLSTAMVAYVLS
                     RKDFFARRSLTALFVITMYVGGGLVPEFLLIRNLGLMNNFLVYILPGLINPFNLIVMK
                     AFIEGLPTELQESAMLDGANDFVIFFRVVLPLCLPVLATIALFVAVGHWNSWFDTYLY
                     CGGNKNLTTLQYELQKILANAAASSTTIDYYSTLDPTKTMRVTPQSLRMAMTIVTTLP
                     IVVVYPFLQKYFIKGMTLGAVKE"
     gene            23306..24967
                     /locus_tag="PK016_01330"
     CDS             23306..24967
                     /locus_tag="PK016_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013431898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL01756.1"
                     /translation="MALVLSFFAGITFSSAKAQEELIKPLTPTAEAGNPATLKVYIGE
                     VNPNDDQFKSPVSQKIKELTGITLEIEYAPNQAAVRDKIQIMAASGDYPDLVYAKGDL
                     SLLKDAGALIPLDELIEEYGPNIKAAYGDNLKRLKWSPEDPHIYCLGITTDNDVTLDV
                     TGGFMLQHRMVMELGYPEIRTLEDFENAIKSYYEKHPDTNDMPTIPLTLCADDWRMLI
                     SVTNPAFQATGAPDDGELYVNPETLECILHYKRPIEKEYFRWLNHMWNSGLLDKETFI
                     QKEDTYKAKIASGRVLALIDAGWAVGEPLTALRRAQEYDKMYGYYPVTVSKEIKQAPP
                     DVRAGYTGGWGIAITKSCKDPVRAIKFLDWMCTEDANILRQWGIEGTHHTYIDGKRVF
                     LPEVEEARHNDPYFGRKTGIGLYVYPWPRFPNYYIDSTGNPVVPDTRKEDIRKNYTDL
                     EKEVLKAYNAEIWKDLFPKPEEYPVKTWGALWMYPTPDEDMRAIGNRLMEHVLRTIPK
                     VVMAPAEEFDKEWDDFLKGLDDLGAEKYEKYMTEQVKKMAEFWSE"
     gene            25066..27393
                     /gene="yicI"
                     /locus_tag="PK016_01335"
     CDS             25066..27393
                     /gene="yicI"
                     /locus_tag="PK016_01335"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HXL01757.1"
                     /translation="MKFTDGYWRLKEGVQAFYPAQVYDYEIKDKSIIIYAPFTIIHSR
                     GETLQGPLFEIHFSSPFPEVIQVKVYHFKGGINKGPDFPLFSDESYSPEIREEEDYLI
                     LKAGRLRIKINKKGYFSFTFYDENKALTGSGFKHLAYIIDEKKNTYMREQLSLSVGEL
                     VYGLGERFTPLIKNGQSVEMWNADAGTTSQLAYKNIPFYITNRGYGVLVNHPELVSFE
                     IAAENVERAQFSVAGEYLNYLIINGPTLEETLENYTRLTGKPSLPPAWSFGLWLSTSF
                     TTNYDEETVTSFIEGMKERNIPLHVFHFDCFWMKEYQWVDFEWDKRVFPDPQGMLKRL
                     KERGLKICVWINPYIGQKSKLFEEGKEKGYLLKKPDGSVWQTDAWQPGMGIVDFTNPS
                     ARRWYSDKLRELIKMGVDTFKTDFGERIPLDVVYYDGSDPYKMHNYYTYLYNQTVFET
                     LKEELGEENALVFARSATVGSQKFPVHWGGDCLATYESMAETLRAGLSLGLCGFGFWS
                     HDIGGFDSSTTPDLYKRWVAFGLLSSHSRLHGNNAYKVPWLYDEEAVEVLKFFIELKC
                     SLMPYLWKEAWEAKEKGIPMLRAMVLEFEEDPTCHYLDRQYMLGDSLLVAPVFSEDGE
                     VEYYLPAGIWTNLITNEKKEGGRWYREKYDYFSLPLMVRPHSIIAIGDRKDVPDYDYT
                     SRVVLHVFELKEGKEVKTWVINQKGEVELELSIIKEGNKISINIEKGYNKEFKIFLNG
                     ISQVERVINGSFAREEKGTFIIPQKECNNIEVILK"
     gene            complement(27729..27815)
                     /locus_tag="PK016_01340"
     tRNA            complement(27729..27815)
                     /locus_tag="PK016_01340"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27780..27782),aa:Ser,seq:tga)
     gene            complement(27848..28945)
                     /locus_tag="PK016_01345"
     CDS             complement(27848..28945)
                     /locus_tag="PK016_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXL01758.1"
                     /translation="MEKIGIGIVGFGFIGRVHALAFSSAPYCYHDLPYLPEIRGIATS
                     RPETAQKVKEETGVALATSSFSELIAQEDIDIVVVALPNALHREVVEEAARAKKAIYC
                     DKPLSSNLEGAEVIERVVKEENVPLGVVFQNRFVPALWRAKELIQEGFLGEILRFRAL
                     YLHSGYLDAERPLTWRLDRKLSGGGALLDLGSHLVDLLFYLGGEEPHLKASWGRTFIP
                     ERKGREIKVDDWSVVLFDLAGGGEGSIESSRISTGSADELRLEIEGKKGAIRYNSMQP
                     NYLAVYDLKDKASPLGGDRGFKALECISQFPPPFALPGKFPVGWINAHVACAHDFLLR
                     LAGREARGATVEDGMKVQRFIEDAYHSMVRS"
     gene            complement(28946..30232)
                     /gene="serS"
                     /locus_tag="PK016_01350"
     CDS             complement(28946..30232)
                     /gene="serS"
                     /locus_tag="PK016_01350"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HXL01759.1"
                     /translation="MLDWKWIKENKEATEEALQKRGMDSEILEELFSEDEKRRTVQRE
                     VDILRQKRNEISRLVASLKREGQDPQDALREGEKIAGKIKEEEEKLREIEEQWKEKLL
                     FIPNIPHSTVPVGKDENDNQEVKRWGEPRDFSFTPRPHWEIGEGLDILDFERGAKIAS
                     SRFTVLKGEGAFLERALINFMLDLHTQKHHYREVFPPFLVNTKSLIGTGQLPKFYEDL
                     YHCQDDLHLIPTAEVPVTNLHSEEVLREEQLPLYYVAYSACFRREAGSYGKDVRGLIR
                     QHQFNKVELVKFALPENSYEELGSLLRDAEEVLQILNLPYRVVALCTGDLGFSASKTY
                     DIEVWIPSQNRYREISSCSNFEDFQARRANIRFRNKEGKMRYVHTLNGSGLAVGRTLV
                     AILENYQEEDGTVTIPEALRPYLRGLKSITPQFETQ"
     gene            complement(30246..31703)
                     /locus_tag="PK016_01355"
     CDS             complement(30246..31703)
                     /locus_tag="PK016_01355"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011929062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HXL01760.1"
                     /translation="MAEAWKGPLQKVDDWRWLIPRTYKKGMLTDGMIYASEKMISAIR
                     KDQAPEQVANVACLPGIVGRSLAMPDIHWGYGFPIGGVAATEEKGGVISPGGVGFDIN
                     CGVRVIKTNLTEEEVKPHMEKLLSLLFSHIPSGVGSKGKLSFSIPELEKVMEKGARYI
                     ISRGFGFGEDEENIEERGAMEGANPEAVSTKARERGKDQLGSLGSGNHFLELEVVEQV
                     FRPDLAEAFGLFPGQVIIMVHTGSRGLGHQVCTDYLQEMQKAMSRYGISVPDRQLACA
                     PCDSPEGRGYFEAMACSANFAWANRQLITHWVREVFEQVFQKSAPALGMSILYDVAHN
                     IAKWEEHEVEGKKMKLCVHRKGATRAFSAGSPLLPPHYRKVGQPVLIPGDMGTGSYIL
                     VGTELAMQETFGSTCHGAGRVMSRSEADRRSKGRDIAGELKERGVLVRAESKATLREE
                     IPEAYKDVGEVVEVVEKAGISKKVVYARPLGVIKG"
     gene            complement(31721..32140)
                     /locus_tag="PK016_01360"
     CDS             complement(31721..32140)
                     /locus_tag="PK016_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HXL01761.1"
                     /translation="MDKFEILDHTADVGLVAWGKTKEELFANAALGMFSLICSVEEVD
                     GGFTTQVEVEGSDYEDLLVTWLNELLYLFEAEEVVLKQFNIQELGQYYLKAQVEGEPL
                     DPKKHHIQRVVKACTYYEVKVEEVEPQLWRAQVYFDI"
BASE COUNT         9509 a         6779 c         7055 g         8893 t
ORIGIN      
        1 tgacccaggg gttaaaaaac cttctcagta atgttctctc ccgattgagt ggaggaaaag
       61 gcgacccggt tattcaaatt caaaccccct tcggcggtgg taaaacccat gctcttttgg
      121 ctctctatca catagtgaaa aattatgacc aggttaaaca tctttctcta ctttctgaaa
      181 ttcaatctcc aatccttaaa gaagcaaaag tagtggtttt tgtggggacg cacgccgacc
      241 cgataggagg aagaaccatc tggggagaaa ttgctcatca acttggagtc tatgagaagg
      301 taaaagagca cgatgagaaa cgacgtagcc caggaaaggg agtgctatat gaagtcttag
      361 gagaagagcc agtgcttatt ctcgtggatg agctggtaga gtatgcggtt aaagcaaaag
      421 actttgccga ccagatttct gttttctctc aggagctaac cgaggcggtt aagtccaaac
      481 ctaatgcttg tctcgtttct acgcttcctt ccagcgctcc ttatggcgag gcaggcgagc
      541 gggcgcttaa tgagctacaa aggatatacg gaagagttga gactgtttat acaccggtag
      601 agggtatcga gatttacgaa gttatacgta agagattgtt cgaagatttt ggagatgaga
      661 aaaccagaaa agaagtggct caaagttatt ttgagctgta tcagaaatta ggaccggacg
      721 tgccctcaga agtaagagaa atagaatatc gagaccggat agaacacgct tatccctttc
      781 atcctgaact tgttgatatt ctctatgagc gatggggctc ttattccact ttccagagga
      841 ccagaggagc cttgaggctt ttagcagaag ttgtcgccga cctttataaa cgccagatac
      901 cctctcccct gatacaatct tctctcgtaa acctggaaaa ccaggctata agaagagagt
      961 ttatcaagca tatcgggaat gaatacgata gtgtaattgc tgcagatata gcgggaaaga
     1021 atgccaaagc ccctaaaata gaccgggaga tgggtagcga atatgaaaaa tatgggattg
     1081 ctactggcat agctacgagt gtctttctct attcttttag cggagcagaa aagaaggaga
     1141 ctactttgcc cagaatccgg gtggctcttt tgagagaggg tatcccatcc accattgtaa
     1201 gcgatgcaat tggcaaatta gaagaggagc tctggtattt tcactctgaa ggaaaacaat
     1261 acgcttttcg taaccagcct aacttgaacc gggtgataat ggatagagaa gagaccatct
     1321 cagaaagtag agttcgggaa aaactccagg atagcatcca gagatatgcg ggcaacgctt
     1381 tagaagttta tctctggcct gaaagtgcat cagatatccc cgacaataaa aacctcaaga
     1441 tggctattct cgctccggag ttttcttata atccagggtt atctgctgca gaacaatcag
     1501 ggaaagtaaa tcagctggta tcggagcttt tggaaaaagc ggggacaggc tttagagtgt
     1561 ataaaaatac gctcttcatt ctggcaatgg atgatgctca gtattctacc ctttacaaat
     1621 ctctaaagcg tctcctggct atctccgagg ttcaaaacga cagaggactt ctggaatcac
     1681 ttactaaaga aagtcagaat gagctgaaga agaaattaaa tgatgccgag aaagaaatac
     1741 cgtttagaat tctcaatgcc tacagacatc tggcgctatt agaggagaaa ggctgggctt
     1801 ggaaagacct gggtatccct atagtgggaa cgagcgttac tatttcagaa agagtcaagc
     1861 aacatctcaa agaccaggaa aggattctct ctcgcctcac tcccaaatac ataatcgata
     1921 gaaccttggc tggggatgaa aatgaaaaag gagcccgaga tatttatgaa cttttcctga
     1981 aaacccccgg gatgcctatc ttagaagacg agagagttct attagatgcc ctaagagaag
     2041 gagtgaggag tggctttctt ggtatcagag aaggacagaa ggtttactac agcgaagacg
     2101 ttagcccgac gatggactct atagttttac gtagcgaagt ggcaaaagaa ataaaagaag
     2161 cagaaaaaga aaaggaagaa aaaggaaaag aagagacgag agagagcgga ggctggaaag
     2221 tacaggaaag atatgaagaa ggaaaggagg aaggaggcga ggaagtaaga gaagagccca
     2281 gaaaaagtgg tgccgttgcg aaattgactt taagagcaaa aatcccctgg gataaactct
     2341 cttctgtagt aggtggtgta gtccggccat taaaagaaaa aggacttcct ccagagatta
     2401 ccatcgagat aaaggcagaa tctcaggaag gttttgataa aatcacgttg gagagcaaag
     2461 taaaagagac tctcaagcag ataggtgcag aaattgaaga gtggagagaa gagtaagcat
     2521 agtaatagtc tgatatactt caggcattgc cttgtatctc tggagcctgc cttaatttct
     2581 ctgtaccggg tatcagatgc aggctctcaa aaaggtcaat ttatggctta ttggtaatat
     2641 aaattataat ttgtacgctt ccacacaatt tcgcagagcc ccagggctct tgcaaagtat
     2701 agattcttgt gttatatttc cttcattagc taaaattata aaagcgagga aagggagtag
     2761 taggcatttt ctctggttac agagagtctg cataagctgg gagcagatac caggaggtgc
     2821 tgaatctcgc cctggagtgg ttggctgaaa gtggagtagg caacccggaa ctctccgtta
     2881 agagaaaaga gtgacttgcc aaggcaagta aaaagggtgg taccgcgggg aaatacccct
     2941 cgtccctcga gggacgaggg gtattttgtt atcataactt agtaagggag gaagcttatg
     3001 agaagtgaaa tagttaaaga gggtccggat cggtgtcctc atcgttcttt attttttgct
     3061 accggtctta ctcgagaatc tttaaataga cctttgattg ggattgccag ttctttttct
     3121 gatttagttc ctggacatat ccatatgcga gagctggaga gatttataga gcggggaata
     3181 gaagcaggag gaggaactcc ttttgttttt ggtcttccag ctatatgtga tggcattgct
     3241 atgggacacg aagggatgag gttttctctt cccagtcgag atgtaattgc tgacggagta
     3301 gaagcagtag ctcaggggca cgccctcgat ggattggttc ttttaaccaa ttgcgataaa
     3361 ataactcctg gtatgctttt agctgctgct cgcctaaatc ttcccactat agtcgtaacc
     3421 ggtggtccta tgctttctgg agtttaccgg ggaagaagac tttcctttgt tcgggacact
     3481 tttgaagctg ccggaaaata tcataaagga gaaattagct tagaagaatt agaagcatta
     3541 gagatggaag cttgtccagg agcgggctct tgtcagggct tatacaccgc taacactatg
     3601 gcttgtctta cggaaactat ggggttgagt ttacccggat gtgccactgc tcttgctata
     3661 tcagctcaga aaagaagaat tgcctatgaa agcgggaaaa gaattgtgga attggtaaga
     3721 gaaaatatat ctgctcgcca gattcttaat gaaaagtctc tgcgcaatgc cataaagatg
     3781 gatttatctt taggaggctc aactaatacg gtcctccatc ttttagcttt agctcaagag
     3841 ctgcaaactt cttctatttc tcttaccact tttgatgagc tatcccggaa aataccccat
     3901 cttgtgatgc tccggccagc aggggagctt ttcttagaag acctccattt tgccggaggc
     3961 attcctgcgg tagaaaaaac tttaggagaa ctcttagaag ataatctcac cgttttgggg
     4021 aaaacggtga gagaaattca aaaagaggta gaagagatct ctatagatag agaagtcatt
     4081 cgcaccttag ataatcccca ttctcctgag ggagggatag cggtgcttta cggctcctta
     4141 gcccccgatg gagcggtggt gaaacagtcg gcggtagaac cagagatgat ggtatttcga
     4201 gggaaagcca tctgctttaa tagtgaagaa gaagctatgg aagctattac taaccagaaa
     4261 gtatccccgg gttcggtggt ggtcatccgt tttgaaggac caaaaggcgg tccgggaatg
     4321 cgagaaatgt tagctcctac ttctgctgtc gtaggtatgg gcctgagcaa aacagtagct
     4381 ttaatcactg atggccgttt ctccggagga actcgaggtc cttgtatcgg acatattgct
     4441 cccgaggctt ttgaaggagg ccccatagct ttaattcggg aaggtgatga aattctcatc
     4501 gatatccctc atcgccaaat agaacttttg gtagataaag aggaattgga gaggagaaaa
     4561 aaagggttta ctccccctct tcctaaatat aaaaccgggc tcctggggcg ttatatcagg
     4621 cttgctcgcc ccacttctca aggagcaagt atggaataaa ggagagtgat gaagttgaaa
     4681 atgagaggag ctcaaatatt aattgagctt ttgaaaagag aaggagtaga agtaatattt
     4741 ggttatccag gaggagcagt gattgatatc tacgatgctt taatggatga taaagatata
     4801 actcatatct tagtaagaca tgagcaagga gcagctcatg ctgctgatgg ttatgctcgc
     4861 actacaggca aggtaggagt gtgtatggct acttcaggtc cgggggctac taatttagtt
     4921 accgggctgg ccaatgccta tatggattca gtgcctttag tggctattac gggacaagtg
     4981 gccaccagcc tcataggtaa agatgctttt caagaggcgg acaccacggg aataactatg
     5041 cccatcacta aacataattt tctggtaacc agtctggaag aacttcccac tgttgttcgt
     5101 caggcttttt acattgcctc taccggaaga ccaggacctg tagttattga cctacccaaa
     5161 gatatccagc agcaagaagg ggaatttgtt ttccccgaaa aagttaaaat acctggatat
     5221 cgccccactt atcgagggca tattaaccag attaaggcag ctgctcaggc tattcaagaa
     5281 gcagaaaaac ctgtcttaat cgctggagga ggaataatat cctccggagc atcggagatg
     5341 cttttgaagt tggcagaaaa agcggatatc ccggttactc atactctgat gggaaaagga
     5401 gcttttccgg aaagccaccc tctttcttta ggaatgctgg gaatgcatgg gacttactgg
     5461 gctaataaag cagtgatgaa ctgtgacctt ttaattgcta taggtatgcg ctttgacgac
     5521 cgggtaacag ggaatctggc tacttttgct cctcgagctc aggtaatcca catcgatatc
     5581 gacccggcag aaatagggaa aaacgttaag ccttctattc ccattgtggg cgatgctaag
     5641 atggtgctct cagaattaga aaaatatgtg gaagaaaaaa aacatcctat ttggagagaa
     5701 aaaatagaag aatggaaaaa agaatacact cctcaagtat ccaaagggct ttccccccgc
     5761 caggtgatag agaaaattta tgagctcacc tgtggtgata acccaatagt agttaccgat
     5821 gtaggacagc accagatgtg ggcagcccaa atatatcgct gtgaatcacc ccgccagtgg
     5881 gccagctcag ggggcttggg aacaatgggt tatggtttac ctgcctctgt gggtgctcaa
     5941 gtgggcaatc ctgaccgttt ggtggtttta atcagtggag atgggagcat ttttatgaaa
     6001 atccaggagt tggttactgc cgttaactat tgtctaccca ttaagatttt tgtcattaat
     6061 aattcttatt tgggtatggt tcggcaatgg cagggttgtc tctatgaagg acgttactcc
     6121 tgtaccaagc ttcaaaatcc tgacttggtc aacttagtcc aggctttcgg aggagagggc
     6181 tttaaagtag aaaaggaaga agagttggaa gaaattattt caaaggcttt agaggtgaaa
     6241 aataaaccag tattggtgga ttgtagagta gtaaaagaag aaaatgtttt gcccttcgtc
     6301 cctcccggaa agtcgctgga agagatgatt ctggaataaa agaaggaggt gcccagtgga
     6361 gcatatcatt tcagttttgg tagagaacag gccccgtgtt ttagcccgag tttcttctct
     6421 ttttgctcgg cgaggatata acatagagag cttagctgta ggtcccacac atgaccctac
     6481 catttctcgt atcactttgg tagtagaggg aaacactgat actctggaac aaataaccaa
     6541 gcagctctac aaactaatcg ataccataaa agtgggagat ttcactcaaa ctaattacgt
     6601 agaccgggaa ttagctctga ttaaagtgaa tgccccctcg gaggtcagag gggaaatagt
     6661 gcaaacagtg gagatattcc gagctcgggt agtggatgtg gaagaaaaga gcttgattgt
     6721 agaggtgacc ggtaccccgg ataaaattga tgccttagag cagttattgc gtaagtacgg
     6781 tatcatagaa atgactcgaa ccgggcggat tgccttagct cgaggaagtc aatctttaca
     6841 agaaaaataa ttaataaaat aaaagggagg ttttattatg agcaaagtat atcatgatga
     6901 ggacgctaat cttcaggttt tgcaaggtaa gacaatcgct ataatcggtt ttggaagcca
     6961 ggggagcgct caagctcaaa atttaagaga tagcggatta aatgttctag tgtcagattt
     7021 acccgggact cccggttttg aaaaagctcg ccacgctggg ttctcccccg tgagtgcttc
     7081 tgaagcggct caaaaaggag acgtaattca aatgctggct cccgaccaat atcaggccag
     7141 tatttataaa gaatcggtag ctcaatacct taaagccgga aaagtgctga tgttttctca
     7201 tggcttcaat attcattacc accagattat ccctcctcaa gatgtggatg tagttatggt
     7261 agctcccaaa ggaccggggg atttagtaag aagaatgtat caggaaggaa agggagttcc
     7321 ttctctcatc gctgtttatc aaaacgccag cggcaaagcc aaagatatag ccctggctta
     7381 tgcccgagga attggagcaa ctcgagcggg agttttagaa actacttttc aggaagaaac
     7441 tgaaactgac ctttttgggg agcaagcggt gctctgtgga ggactgactg ctttaatcaa
     7501 agctggtttt gaaactttag tagaagctgg gtatcaacct gaagcagctt actttgaatg
     7561 tctgcacgag atgaagttaa ttgtggattt ggtatatgag ggaggactca ccaatatgcg
     7621 ccaggtggta agtgacactg ctgaatacgg tgaccttacc cggggtcctc gagtgattaa
     7681 tgatcaagtt aaagaagaaa tgaaaaaaat cttaggtgag attcaggatg gaagttttgc
     7741 tcgggaatgg attctggaaa atcaagccaa tcgaccagtg tattatgctc tgcgaaataa
     7801 agatattcaa catcccatcg agaaagtagg gaaaactttg cgagacatga tgccctggct
     7861 caaaaaatga gggggtagga taatgagaaa tatccggatt tttgacacta ccctgcgaga
     7921 cggggaacaa tccccggggg taagccttaa tcctcaagaa aaattagaaa tagcaaaaca
     7981 gttatctcaa ctgaatgtag acgttataga ggcgggcttc cccatcgcct ctcccgggga
     8041 tgctgaggga gttaaattga ttgctcagca agtaaaagga ccggccatat gtgctctggc
     8101 tcgatgcaaa gaaaaagata ttgaggtggc aggagaagca gttaaatatg ctgaacgacc
     8161 ggttattcat gtgtttttag ctacttccga tattcatctc cagcataaac tgcatcttac
     8221 cagggaagag gctttagagg aagcagtctg ggcagttaag aaagcccgga cctatactaa
     8281 tgaagtggaa ttttctgctg aagatgccac tcgcactgat tgggaatatc tctcccagat
     8341 ttatgctgcc gtgattgaag cgggagccac aactcttaat attcccgata ctgtgggcta
     8401 cactatccct gaggagatga aagagctaat cctttatctt ttagaacaca cccccggtat
     8461 ggacaaagta ggggtgagtg ttcactgcca caatgactta ggattggcca ctgctaattc
     8521 tttagcggca atctctgccg gtgctactca gatagagtgt accataaatg gtattggaga
     8581 aagagccggg aatgctgcct tagaagagat agctatggcc ttgtatgtgc gacaagaccg
     8641 cttccaagct caaacttctc ttaacttgca agaaatatat cgcaccagcc gcttagtgag
     8701 taggcttacc gggatgctcg tgcaacccaa caaagccata gtgggacaga atgcctttgc
     8761 tcatgaagcc ggtattcacc aggatggggt gcttaaagac aaattgactt acgagataat
     8821 ggatgctcaa ctcatcggta gagaagagag agtgctggtg ctgggaaaac actccggtcg
     8881 ccatgctttt gctaaaaaat tagaagattt agggtatcag ctttccgaag aagagctcaa
     8941 tcaggccttt cagcgtttta aacagttagc tgaccagaaa aaagaaatta ccgaagccga
     9001 cctcaaggct attgtttctg aagaggtatc tcgaccagag gaaatgtatc gcctggattt
     9061 tgtgcatgtt tgctcgggga ataacatttt acccaccgct actgtgagac taattaagga
     9121 agatggagaa atattagagg gggtatccac cggagttggc cctgtggatg cagcttacaa
     9181 agccataagc aaaatgctgg gtatctctcc caatttagtt aattacactt tgcaatctat
     9241 taccggagga acggaagcgc tgggagaagt aattgtgaga ataataggag aagaccagga
     9301 atatgtagga agagggtcta accaggatgt ggtggtagcc agtgttttag cttatcttgc
     9361 ggctataaac cgaatggctc aaaagagaaa gaaaatcaac cctcaaaaag attataagga
     9421 gaactaaaat gggtatgacc ataaccgaaa aaatcatagc tgctcacgct caaaaagaaa
     9481 aagtagctcc tggagaactg gtgcaggtaa aagttgatct ggctttagct aacgatatca
     9541 ctgctcctct gggtatcaaa gaactggaga aacaggggat tgaccgaatc tttgaccctg
     9601 atagggtagt tttagttgcc gaccacaaca ctccttgcaa agatattgct tcggcccaga
     9661 atgtaaagtt gatgcgagat ttcgctcgaa aatatggtat atctcatttt tttgagggag
     9721 gaaaggtagg aatagaacat gtgctgctcc ccgaacaagg tctgatagtt cccggggatt
     9781 tagtaatcgg agccgattct catacctgca cctatggagc gctgggagca ttttctaccg
     9841 gaatgggaag caccgatatc gccgccagct gggtgttagg agaaacctgg ttgcgagttc
     9901 cagaatctat tctctttctc tatgaaggga aaagaaaacc gtttgtgacc gggaaagacc
     9961 tcattctttt taccatagga aaaattggag tagacggagc aagatattgc gctatggaat
    10021 ttagaggttc ggtgatatcg gagctatcct tggacgagcg ctttactatg gctaatatgg
    10081 cagtagaagc tggagcgaaa aatgggataa tggaagccga tggtaaagtt ctcagctata
    10141 tttccgagag aacttctcgc ccttgtgtgg tttaccaatc cgactctgat gctcaatgga
    10201 gagaagtaat agagattgac gtttctcctc tggagcctca agtggccttt cctcacttac
    10261 ccagcaatac tcattccgtt tattctattc ctaaaataga aattgaccag gtagttattg
    10321 gctcttgcac caatggccgt atggaagatt tgagaatggc agcgcaagta ctaaaaggcc
    10381 gggaagtcaa ccccggggtg aggcttttga ttttccctgg aactcctgcg gtgttaaaac
    10441 aagccgaaaa agagggattg ttgcgtattt tcttagaagc aggaggaata attgctcctc
    10501 cttcttgtgg gccgtgtctc ggtggacatc tgggggtttt agccgaagga gaaaaatgtg
    10561 tatctaccac caaccgaaac ttcttaggga gaatgggaca tataaagagc gaagtttatc
    10621 ttgctaaccc ctatgtggca gcgagctccg ctattgccgg ctacattgct gctccggatc
    10681 aaatctaaga cgaggtgata aaatgataat cagcggaaaa gccctgaaat atggggacaa
    10741 catagatacc gatgttataa tcccggcttg ctatctcaca tctactgatc ctgcggagtt
    10801 agctcaacat tgcttagaag gtttagaccc caccttctcc gagaaagctt cctctgctat
    10861 cctggtagca ggaaaaaact ttggttgtgg atcctcccgc gaacatgccc ctttggctat
    10921 taaagcatca ggagcgaagg cggttattgc tgcttctttt gcccgcatct tttatcgcaa
    10981 tgctataaat attggtctac ccatattaga atcagaagaa gcagcagaaa aaattaaaga
    11041 aggagaccaa ttagaaattg acctggatag aggagaaatt aaagacttaa ctagtggaga
    11101 tacttttaaa gttaacccct tccctccttt tttacaagag ataatagagc ggggaggatt
    11161 gatgaattta gtgaaagaaa gagttaaatc tagctaattt ttcaggctgg taatgggagc
    11221 gggcttttct cctggaagat aaagtttttg ctctggaagg gttaacttct ataaaactct
    11281 tgtcttcctg gagagctccc gtattacggt taaatttgtg gttaagtgat acgcttattt
    11341 ttcttcctgc ttcaggaaaa taagggctcc caggagaaat cctcccccgg tatagcttcc
    11401 cagaactaaa aaagagatga aagcttgatg ccagtcaatt tgggggagga aaaagacatt
    11461 gaaagaagaa agatgatggg cgagaaggta aatttgaatc ctttctcctt ctcccagatg
    11521 ctctattatg taggaaaaag ctcccacccc taaagataga agaggacctc cccagggacg
    11581 atagaaaaga gaagaaaata gataagataa agcagaaaaa gagctaatgt aaagtgtaac
    11641 cactaatcct cctcctaaaa ggcggaaaga gaaccctcct ccccacataa ttttctctaa
    11701 tagtgccatc cccaaaagag gagtgaaaac cataagtaga ttgtaaaaaa gagtgaactc
    11761 tacctttccc caataccact cccagcgaga gagtgggcgc aaaagagcag tggagagagt
    11821 tccttccatt ctctcttcgg caatattttc tgttcctccc cagattcctc ccaccggacc
    11881 aaaaagcgcc agaggataaa gaaaagaaaa ggggagagta atttttctca gataaaatga
    11941 gagcgggtcg cgccagactt gagctaccac caaacctgcc agagcccagg aaatcaaaat
    12001 ccctccatag aagcgggggc tatgaaatgc ttttttaatc tctgctctgg cgactttcat
    12061 tttccctcta ttatctccca gtaaatttct tctaaagaaa cctgaggagt taaataatca
    12121 ttccacttta ttttaccccg gtgcaaaatg gctattcttt gagccacttt ttccatctcc
    12181 tggggaagag gagaagaaag gaaaaaagtt actccctcct cggtatttaa agttaaaatg
    12241 aactcctgaa ttttttttgc tccctggggg tcaagtcctg cccatggttc atcccagact
    12301 aaaatccggg gaaggggaaa aagggaatgg agaagggaaa tcttttttct ttctccctga
    12361 gaataagtac ttactttttt ataaagaagg gatgactcta aacctagaag agaagcaaaa
    12421 aactctatct cctttaccct ggggtagtag gaagacagag aagcaaaaag agtgatattt
    12481 tctattccgg ttaaattccc gtaaaaggaa gtgttatcca ggcatcctcc tattaaagct
    12541 tttgattcag gaaggcaagg gggacaacgg taatttccca cataaaaaca ccctgcagag
    12601 ggcgaaacca gaccaaaaag aatacgtatc agggtgcttt tacctgctcc gtttcccccc
    12661 agaagtccga atatctctcc ttttctgact tcaaaagata cctcttccag aatagctctc
    12721 ttccttgctt tcttttccac attttttagt tggaggataa tatcatcttc catgggtata
    12781 tttaccagaa ttttcttctc tttctcccgt ccagagaaaa aaagcttcat caaagccttt
    12841 ttcctgaacg gtggagaaaa aagaaggaag gttttcctct cctatctttt ccttaaaaat
    12901 ctggaaagat tctcgacact cccgataaaa ctcacctgta ccttcctgga aatattctcc
    12961 agaggcggga aaaaagaaag aagccagacc ttcttcaaac cagaaaggga gctgccagtt
    13021 atagcttccc acaaaaatat ggagaaattc atgagccagg gtggaagaaa gaatccctcg
    13081 ctcaagaagt aaaagaggga agcgaagata aatagtatta cctcgggaaa aagctgctac
    13141 ccaggagggc aaagctttag aggtttctga agataaaacc acgatacgga aagaagaatg
    13201 aggagagtaa ccggtcactc tttgaaaaca aagttgagct tcttcccagg ctgactctac
    13261 catgggaagt aaaaagggga agctctcccc gccttctatc acgaaggtag gaagagcccc
    13321 tcccgttcga gataaaaaaa gaagaatcac cactcccagg agaaaactcc ggaagcagga
    13381 attattccac caccagattc ttttcttctc cataatctct aacttcaggg aaatacatga
    13441 gataaagaag gggtgccttc acatagaaat ttccctggtc tcggattctt aagtagtagc
    13501 gaattacatt tttccccgga agaaggtcat acacaaagag agtcggttgt ccctcaaaaa
    13561 gagtttttac cacaaaagga taatgcactc cttcttcctc aggaagagaa aaggcatagt
    13621 cttttcttat tagctcgcaa ccagcaggca tcccgtcttc tatcaccagg tattctaaat
    13681 aatcgggact ctgaatttct atctcacaaa ctatttcctc ctggggatgg aagatatcca
    13741 cttcctgagg ggagaagaaa acttcttctc ctttggtggg tattaaaaga taataatttt
    13801 tctttaccgg gaaaaattga gatggaggag cttcttcctt ccagcttttt actttgagct
    13861 ctgccatgaa tagattttct ccctctatct ccaggtgatt ggagccctcc tgcaaaaatt
    13921 gcccgggaat agctattttc tcttctcccc ttccttcttt aaccagcgct ccattacaaa
    13981 gcacccggaa agtggtactg ttactctctt cttttagaaa gtgggagcta accaggaaaa
    14041 agtaagcccg ctccagagta gtaaagaaat ttcctccctt tttgctccgc cacagccaca
    14101 ggtaagcttt ctctcccagg ggattttttc tctctcctaa gaataaggca gaaaaagttg
    14161 tagccgaaaa acggtcatct tcccagggtt tttgagggtt aaccgttccc cagaacactg
    14221 tactgcctaa agcatgagct cttttttcta attctttttc cactttctcc tggtcttctt
    14281 cccgggttat caaaccccat agagctaagg ttttatcatc taactctgct aaaggtggaa
    14341 gggaaggagg aacaaagtct ttcttttcca gagaaagaac ataccaggat agagcttttt
    14401 caaactcgct cgggagtgga aggaaagagc cttcaggaaa aacctcactt ctggaagcat
    14461 tatcctctaa aaatcttaac cctcgctcta ttgcttcatc actaactaaa aatcctctat
    14521 tttttacttg atgcaaagct aaaagagtat aggcagtgtg gaataaatct ccttctcctc
    14581 cccagaaacc ccatcctcca tcataatttt gcaggttgta aagggtataa aggtcttctc
    14641 ttattgcttt tactaactca ggagttttat cttcttccag aggtagagag gaaaaagtaa
    14701 tcagccgact ggctacttgc tcgctgcaac gataaggata ggaaagcata tattctatct
    14761 cttctaaaag tacactgcga gggtcagaaa acaaagtaag ctcgacggtt gaggaagaat
    14821 cacaggaaaa ctcaaaagag gttttccctc tttctaaaaa ggaagagaaa ttttcttcta
    14881 cctctactct tttttctttt acctccagag gtaactctag agcatcttgt gccttttctc
    14941 cttgagcaaa aagccgtaga tatgcttttc ccggagactg ggcactaatg agaagcggaa
    15001 ataccgcttc ctccggggca gatattttcc ggggctcttt ttcccattgc agggaaattt
    15061 ccggggagct ttctccttct atcaccactt cctggggtgc tacttcattc cagagagtaa
    15121 ccttgctttt cagggtatct ccttctacta gaaatgaagg aagttccact cctaaagcaa
    15181 agggtttata agtagtaaag tactcctgtg cttctccaaa aaactcctcc cggtgagctt
    15241 ttacttctac ccgccagcga gtaagagaat gaggagccac aaaatccact tctactttcc
    15301 cttcttcgct ggttgataag tttggctccc ataaaagagt atcgggaaaa tattttctta
    15361 acagtacttc tcccgctcct cctccatttc ctttaaactg ggctttcaac tgggctttca
    15421 tatcctggaa aggtatacct cttccgtaga aggaagaata aacagagtaa gaaccataaa
    15481 ctgagttata aaattgagag tagaagtagt catatatgtt gtttatccac caggagctca
    15541 ggtcaaaaat agcttggtct actatagcta gggacagctc gcatttcact ggttttcctt
    15601 gggtgtcact cacctgaata gagattttac ctttttctcc gggttgatat ttctcccggt
    15661 caggagttat ctttatctgt aattccttct ctcgctttag aggtataggt aaagattcag
    15721 aatataattt tccgtcgcta aaccccgaaa aagaaacata agctcccatt acataagaag
    15781 gtaaaatcgg gataacgatt tcttccggtg cagatatttg ttttacttct gcttcttgaa
    15841 gattgggacc ctctatggaa aagagaaagg tgaaagttcc ttctccggga ggaatgatta
    15901 aaattcgggc tttctcaccc acctgataac gctccttatc ccagcgtact tctaactcct
    15961 gggaaggata gtataaatct tctcccgtta cccagaaata agaagaggcc actattttat
    16021 tgcctgactt atcctgggta taaacctcca ggcgataata gcctgcccgg tctaaagtta
    16081 cctttatctc tcctgtgtcc tgggagagag aaacatttaa aaattgagaa ggaatttctc
    16141 tccatttctc tttttctttt tcccactcta atcgatatac ctccccatag actttttcta
    16201 ccgctacttt tttctcttca gcgtcccgag cctctacttc ataggtcata gtttctcctg
    16261 cttttacaaa gtaagcttgg ggttgcaaag agagatagaa ttctcccatc aagactcgaa
    16321 cttcttcttg tttttctacc gcccgataag cggggtcaaa tactacgcag cgaacctggt
    16381 agcgaaaagc tccctctccc tccgggagaa agcgtatttc cccctctcct ttttcgtcta
    16441 attttccttc tccttctccc catatctctt ctcctctttc ttctttccag taaccaatag
    16501 gatagcgaaa tatctgataa cggaattcac ccccacttaa agggcgctgt gagtaatatt
    16561 ctccttttac tgaaaagtgg acttcttctc ctttagcata gactggtttt tctggttcta
    16621 attggacagt aaaaactggt ttttcataat tttcaatgga aataaactgg taaaattctc
    16681 tatctctcca ccgtagccgg attaagaagc ccccttcttc aattttttgt ggcagagtga
    16741 aggaaaagct cacactgcct tcggaagtag aagaaagagt ttctttcccg tattcattat
    16801 cccggtaatc taccagagct acttctactt tttctcccgg aggaggaaca atatatctat
    16861 cggtgtttgc ttctcgcaaa atagcttttc ctaaaacagt ttgtcctggc ttatacaggg
    16921 gacgctctga atagataaaa gcttttaatt ttccttcttc ccagtaataa ggatagaaat
    16981 tcaaaaaagc gaattcttta tctttctcta ccagaataac tgcttcttct ccttcttccc
    17041 atcttttctc ccagaaataa agtccctggc tatcggtaaa tccctcttct ttttccttac
    17101 cctgccagat tttaacctgt gctccctctt gggggatacc agtttttaag tcctggacca
    17161 agataacccc ttttttctca tcctgacggc aaaggcagga taaatgggtt acgctgaaaa
    17221 gaacccgagc ctctcttccc cctccttggg cagtgaataa gtaaactccg ggaggcaagg
    17281 ggttgatttc tatctctctc cagaaaaaag gagaatcttt ctcttgaggt agatctaccc
    17341 agaaagtttt tagctcatca gttccccggc gaaaatcaga aggagtaata gtgatagggc
    17401 ggcgaggtaa gggtccctga atatcaaata ggtcgcgcaa aaagtctttc accggggtag
    17461 gtagcacccg agaagcaagt tttcgcactg cttgaaagaa agaatattta gtattttgaa
    17521 actcttcttc ctcctctaag gggcgatgga gttgccaact atctacctga aagaaagatt
    17581 ttgtttctac gctctggata tattgctctg ggtcattaat ccgataaagg gaacactgtg
    17641 ctcctttcgt ttcccggcca ctgatattta tttttattat ctccccagga tagatatgag
    17701 ctgggacact aacatatatt tcctcttgag ctaaaacctt tcctcctaaa aagagtaaaa
    17761 aaattgccac ccaccaaaaa agaattttta ttttcttcat tttttctccc ctctaatcta
    17821 aaattttcca gcggaaaaat cccagaaagt tgggattatt tttcgcagga tgccagtttt
    17881 tatcgggatg cctccggaga gaggcaagag tagtctctct tactccctct ccctctccta
    17941 tggggccagt atggtaaatc acccttcctt ctccctcata aatcataaca tgataaggat
    18001 aagaaaaaga ggtgggctgg aagaaaaaga gaaggtctcc cgctttcgct ttttcttctt
    18061 ctttccccag aggcacgaca ttataagcca gaagataaaa agaagaggca aaaactgaaa
    18121 aatcttcttc tattttatcg gggctaaaac cacccggttg aatcctaaaa atattaaccc
    18181 ctagtaaagg aacttgaggg taggtgtagg gaagaatatc acctcttttt aagctttctc
    18241 ggtaggcaaa acgaaccaac cctccacaat ctctttccgt aaattgggaa tgaggagagc
    18301 tgatttgcag acgagctaca ttaacaaaaa actctcggaa agcgtctcgc tcgctttcgg
    18361 tgttcagctc caccatatcg ggaaaaccat ctccatctct atcctccaag ttgggaaaaa
    18421 cttccacctc cactttttgc cagactggaa atacccccac tagcagggag gttttccctt
    18481 ctaaccgctg gggctggaag ataacctttc tcccttcctg gcttacaagg attagctctt
    18541 catcttcttt taccactctg actctttgaa accaggaagg aaaaccccac aaagtaagag
    18601 gctctatttc ttcaactttc gcttttcctc ctgcttgaag atgcaggtaa gaagggcagt
    18661 tgagtttaac aggaaaaata agtgttaaaa tccccagacc caaaatgata aaaagtacta
    18721 cctttttcgt attttatccc tctattctaa aattgcttcc cagggggata aaaaatctac
    18781 ccaggaaact ttttgataac cttccccttt ttgaggaagc tcaagagggg tatccttttc
    18841 ttttgcttcg tagacacgta aagctagttc ttcattttcc tcttttgcca cgattagccc
    18901 gttaactttt attatctccc tccagaaagg aagggataat ccttcttttt ccagaatttc
    18961 tactacccct cctaccattt ctcccatggt ggggtggata ccctccttga agtaccatcc
    19021 agttatctca tcgttaacgc gaaaaggaaa acaatccccg aaaggaggaa gagataaagg
    19081 ttcaggaagg agaaagaaag aaggtttttc ggcaaaaagc cattcttctt tcactcctaa
    19141 aatggtggat agggcaaaaa gaaaagaaag agcgataccg tttttgtcct ctaaagtaga
    19201 gaaggtgaga tttaagtcat caatcaggga aagttcaggc ttggtaaggt ctacccctac
    19261 ccggaggata cttttttctc cctgctggag caagagcacc attttttctt cctctttttc
    19321 ccaggaaaat tgataaactt cctcctgcca tttaccttct ccttctatta tccagtgagc
    19381 ccaagagaaa gagttacaaa gcaacacgga cagtccaaga gcgataaaaa taaagattat
    19441 ttttttcacc aaaattcctc ctttatctac ttctcaacat ctctatcact aaaaatatcc
    19501 ccagtaaagc agctattcca aaacctaaaa ttcccagaat acccatagct ccaggccggg
    19561 gagagctgat atatatcagg gaagagccaa tgagtaaaga ccctaaaatg actgaaagag
    19621 aaaggcggtt acttacccgg tctaagcgac gattcaaatt ctttaagtct tcactctcca
    19681 tctggaagcg aattcttcct ctggatacct cttctgataa ctcctgggct ttgtcaggaa
    19741 aattagccag taatttttgc cagtcaaaaa gatagtcagt aactttctct gacaatctct
    19801 cccaccggag gcgttgggca aaagttcgtt ctaaggcttt ctctaaataa ggaactacgg
    19861 tgaagtgagg atcgagcatc attcctgttc cttctaccac cataagagct ctcataaggg
    19921 aagaaagatg gggagggaaa cgcaagctat gtttacgtaa aagataaaaa aagtcattaa
    19981 ccaggctgga aatattcatt tcctttaagt tagcagttac atatcgctct aaaatctcat
    20041 ggaggtctac ccggagagta agaggggatt caactggaga gagcaaaaac tcgtcttcta
    20101 aaatatctac cactcttttg gtatctttcc gcaatacagc gagcaacatt ctttccatat
    20161 tttccctggt tctctcatcc accgttccta ctattccaaa gtctaaaaaa actaattctc
    20221 cagtttcttc tctaaccatt atattgccag gatgagggtc agcatgaaaa aaaccgtctt
    20281 caaaaatcat tttgagaact gcctctgctc ctcgacgggc taaaaatgga ccatctatct
    20341 gaagctgtcc tttttctgcc atctgagaga gattaaagcc ttcgatcaat tccagggtca
    20401 aaaccctggt agtggtgtac tcatcaaaaa cccggggaat gtagactcct tcaaaatcag
    20461 aaaaattttc tcgaaatcgg cgagcattca ctgcttcata caaaaaatct atttctctca
    20521 tcaaactctt ctggaactcg gttaccattt ctgagaaagt gagaggagca aaagaagtta
    20581 accggtcagc caaattggct aaataaaaga ggatttccag gtcggaaata actttttctt
    20641 ccaccccggg tttttgaacc tttactgctg ctttatttcc atctttcaaa actgctcggt
    20701 gcacttgagc tatagaagca gaagcaagag gcgcagggtc aaattcggca aaaatttcct
    20761 cgataggttt cttaagctcc tgttctacta cttcccgtat ttcgaggaaa ctcaccggag
    20821 aagtctggtc ttgtagcttt ctcaattctt gacaatattc ttcgggtaaa aggtccgggc
    20881 gagtgctcag aatttgcccc agctttataa aagtagttcc cagctcttca aaagccaaac
    20941 gcaggttttc ctgcggagta cgcttagtag tctctttttg gaaaagagga agagtgagct
    21001 gttcgggaaa ttgagagcgg cgaaaaagat ataggaagcc atatttgcga aaaacccgga
    21061 taatttcccg cacccgacga aaattcctga tatgacggcg accaaaagga atcatctgtt
    21121 acctcgctat aaaagcttta atactatttt accagatatt gattcctttt tacgcttaat
    21181 ttatttaaag ttgttaacac tgaggaaggt ttttcttccc cagtagcttt tactttttgt
    21241 ctaatttcca aggatgaaaa tgtctaaagt ttccggtttt attaaaaatt ttgtctggta
    21301 tcttttaatt aagtcttaaa aaagagaggg ataaaaaata gtcacgataa aaaccctcaa
    21361 aagacagaaa tacttaataa taatgattct tccctttgta atctggcttg ttatttttag
    21421 ataccttccc ctatggggct ggattacagc cttccaaaat tataaacccg gtgttcctct
    21481 atttcaacaa caatgggtag gcttgaagta tttcaaggag atgtttagcg accctgagtt
    21541 ctatctggtt atgagaaata ctctggctat gagtcttctg gggcttatct ttggttttcc
    21601 tctgcccatc tttctggctc ttttagtaaa cgagataaga aatgaatcct tcaaaagaac
    21661 ggtacagact atctcttacc tccctcattt tgtgtcctgg gtaattgtag caagtatagt
    21721 tcatgctatg cttgctcctg acggagttat taattacctg ctcatacaaa taggtcttat
    21781 cgaagagcct atactttttc tgggagaacc cagatacttc tggtggattg tagtatttgc
    21841 cgatttatgg aaagaattag gctggaacac cataatttat cttgctgcta ttgccgatgt
    21901 taatcctgcg ctatatgaag cagcagaaat agacggagca ggcaggttca aaaaaatgcg
    21961 gtatattact cttccctgca taatgcctgt ggttacggta atccttatcc tctccatagg
    22021 aaatatcata aatattggat acgaaagaca attcttgctc cggacacctg cagtaagaga
    22081 agtttctgaa gttatcgacc tttatgcttt agattatgga attagagcgg gaagattttc
    22141 ttttggaact gcagcaagta tgttcaaatc ggtggttagc ttaatcttgg tattttctgc
    22201 caacagattg agtaaaagag tttcagatta tagaattttt taagaggagg gattagaaat
    22261 cactactaaa tctttttctt caagagataa ggtctttgat atagtaaatt acattataat
    22321 gaccttgctc gccataataa ctctttatcc ttttttatat gtactggcag tatccttaaa
    22381 tgaccctttt gatactataa aaggcgggat tgttttattg ccaagggtat tcaccctgga
    22441 caactacaaa gaaatcttta actacccttt aatagggcag gctactttaa tatcaacctt
    22501 gagaactgtc atcggaactg gtacaagtgt gttatctaca gcgatggtgg cctatgttct
    22561 cagcagaaaa gatttttttg cccgacgaag cctcacagct ttatttgtga ttactatgta
    22621 cgtaggggga ggattggtac ccgaattcct tcttattaga aatttaggcc tgatgaacaa
    22681 tttcctggtc tacattctcc ccggtcttat caatcccttt aatctgattg ttatgaaagc
    22741 ttttattgag ggtcttccta ctgaattgca ggagtcagca atgttagacg gagctaacga
    22801 ttttgtcata ttttttagag tagttcttcc tttgtgcctt cctgttctgg ctaccattgc
    22861 tctttttgta gcagtgggac attggaattc ttggtttgac acttatcttt actgtggagg
    22921 gaacaaaaac ttaaccactt tgcaatatga gcttcaaaag attctcgcta acgctgcggc
    22981 atcttctact actattgact attatagcac ccttgaccct acaaagacta tgagagtaac
    23041 acctcaatct cttagaatgg ctatgaccat tgtaactacc cttcctattg tagttgttta
    23101 tcctttcctg caaaaatact ttataaaagg tatgactttg ggggcggtaa aagagtaaaa
    23161 gaagaaagga ggtggaaaaa gccaccagta gttcttcgct ccctgtaaaa gggaagtagc
    23221 ggtaatttta ttgtttgacc ccgagtaaaa aataaaaatt aaggaggaaa acgaagtgag
    23281 aaaattaaca ggtcggattt tagtgatggc tctggtttta agtttttttg ccggtataac
    23341 ttttagttct gcaaaagctc aagaggaatt aattaaacct ctcactccta ccgctgaagc
    23401 tggaaatcct gcaactctta aagtatatat tggagaagtt aatcccaatg acgaccaatt
    23461 taaaagtcca gtatctcaaa aaataaaaga actgaccggg ataactttag agatagaata
    23521 cgctcccaat caggcagcag tgagggataa aattcaaatc atggcggcaa gcggagacta
    23581 tcctgactta gtatatgcca aaggagatct atctctttta aaagacgcag gtgcgctaat
    23641 ccccttagac gagctgattg aagaatatgg accgaatatc aaagctgcat acggagataa
    23701 tttaaagagg ttaaaatgga gtccggaaga cccccacatt tattgcctgg gaataaccac
    23761 tgacaatgac gtaacactgg atgttactgg cgggttcatg cttcaacata gaatggtgat
    23821 ggagcttggt tatcccgaga ttagaaccct ggaggacttt gaaaatgcca taaaaagcta
    23881 ctatgagaaa catcctgata ccaatgatat gcctaccatt cctcttactc tctgtgctga
    23941 tgactggaga atgctcataa gtgtaacaaa cccggctttc caggctacgg gagctcctga
    24001 tgatggagaa ttatatgtaa atcctgaaac tttagagtgc atacttcatt acaaaaggcc
    24061 catagaaaag gaatacttca ggtggctaaa ccacatgtgg aacagcgggc ttctggataa
    24121 agaaactttc atccagaaag aagacactta taaggccaaa atagccagcg gaagagtctt
    24181 ggctctaata gacgcaggct gggcagtagg agaaccgtta acagctctga gaagagccca
    24241 ggaatatgac aaaatgtatg gatactatcc tgtaactgtt tctaaagaaa tcaaacaagc
    24301 tccgcctgac gtacgagcag ggtatactgg tggatgggga atagctatta ccaagagctg
    24361 taaagacccg gtaagagcga taaaattctt agactggatg tgcactgaag atgctaatat
    24421 tttgagacag tgggggatag agggaacaca tcatacctat atagatggta agagagtttt
    24481 cttgcctgaa gttgaagaag caagacacaa tgacccatat tttggtagaa aaacgggaat
    24541 tggcctatat gtatatccct ggccgagatt tcccaattat tacattgact ctacaggaaa
    24601 tccggtggtt ccagacacta gaaaagaaga tataaggaaa aactatactg atttagaaaa
    24661 agaagtcctc aaagcttata atgcggaaat atggaaagac ttgttcccca aaccagaaga
    24721 atatccagtt aagacctggg gagctctgtg gatgtatcct acacccgatg aggatatgag
    24781 agccataggt aataggctta tggaacacgt cttgagaact ataccgaaag tagttatggc
    24841 tccggctgag gaatttgata aggaatggga tgactttctt aaagggttag acgatttagg
    24901 tgcagaaaaa tacgaaaaat atatgaccga gcaagtaaag aaaatggcgg agttctggag
    24961 tgagtaaaat gtaggcatgc tcctggaggg cggggaaatt tccccgtcct ttttcgtttt
    25021 aaagtgttaa aatcaagatg gttaagtctg aaaggaggct aatatatgaa atttaccgat
    25081 ggatactgga ggctaaagga aggggtacaa gctttttatc ccgctcaagt ttatgattat
    25141 gagattaaag ataaatccat aatcatttat gcacctttca ctataatcca cagtagaggg
    25201 gaaactctgc aagggccttt attcgaaatt catttttctt ctccctttcc tgaagttatt
    25261 caagtgaagg tttatcactt taagggagga attaataaag gtccggattt tcctttattc
    25321 agtgatgaga gctactctcc agaaattaga gaggaggagg actatctaat attaaaagca
    25381 ggcagactga gaataaaaat aaataaaaaa ggatatttct ccttcacttt ctacgatgaa
    25441 aataaagcct taacaggttc aggtttcaag catttagcct atattatcga tgaaaagaag
    25501 aatacctata tgagagaaca actatctctt tctgtggggg aattagttta tggattagga
    25561 gaaagattta ctcctttgat taaaaacggt caatcagtag aaatgtggaa tgccgatgca
    25621 ggaactacat cacagttagc ttacaaaaac atcccttttt atatcaccaa cagaggatat
    25681 ggagttctgg tgaatcaccc tgaactggta tcctttgaaa tagcagcgga gaatgtagaa
    25741 agagcgcaat tcagcgttgc tggagaatac ctgaattatc tgatcatcaa tggacccacc
    25801 ctggaagaaa ctctggaaaa ttatactcgc cttacaggaa aaccttctct tcctccggcc
    25861 tggtcttttg ggctctggct ttctacatcc ttcaccacta actatgatga ggaaacggta
    25921 accagtttta tagagggaat gaaagagaga aatatccccc tccacgtttt tcattttgat
    25981 tgtttctgga tgaaagaata tcagtgggta gattttgaat gggataaaag agtgtttcct
    26041 gaccctcaag ggatgcttaa aaggctgaaa gaaagaggtt taaaaatatg tgtctggata
    26101 aacccctata tagggcaaaa atctaaactt tttgaggaag ggaaagaaaa gggatacctt
    26161 ttgaaaaagc ccgatggaag tgtatggcaa actgatgcct ggcaaccggg aatgggaatt
    26221 gtagacttca caaatccatc agcccgcagg tggtattccg ataaattaag agagctaata
    26281 aagatgggag tggatacttt taaaaccgat ttcggggaaa gaatccctct ggacgtagtt
    26341 tattatgacg gttcagaccc ttataagatg cataactatt atacttatct ttacaaccag
    26401 actgtatttg aaacattgaa ggaagagctg ggggaagaga atgctttggt atttgccagg
    26461 tctgctacag tgggaagcca aaagtttcct gtccactggg gaggagattg tttagctact
    26521 tatgaatcga tggctgaaac cttaagagcc gggctgtccc tggggctatg cggatttgga
    26581 ttctggagcc atgatatagg ggggtttgat agctcaacca cgcctgacct gtataaaagg
    26641 tgggtggctt ttggactttt atcctctcac agcagacttc atggaaacaa tgcctataaa
    26701 gtaccatggt tatatgatga agaagcagta gaagtattga agttttttat agaattaaaa
    26761 tgttctctta tgccctattt atggaaagag gcctgggaag ctaaagaaaa aggtattccc
    26821 atgctcagag ctatggtttt agaatttgag gaagatccta cctgccacta tttagacagg
    26881 caatatatgt taggagattc tctattagta gcgcctgtat tttcagagga cggagaagta
    26941 gaatattacc tccccgcagg tatctggacc aatcttataa ctaacgaaaa gaaagaaggg
    27001 gggagatggt acagggaaaa atatgattat tttagccttc ctctgatggt tagaccccat
    27061 agtattattg ccatcgggga tagaaaagat gtccctgatt atgattatac ttcccgggta
    27121 gttctccacg tttttgaatt aaaagaggga aaggaagtaa aaacctgggt aataaaccag
    27181 aaaggagagg tagaattaga gctaagcata ataaaagagg gaaataaaat ctctataaat
    27241 atagaaaagg gttacaataa agaatttaag atattcctga atggaatctc tcaggtggag
    27301 agagtaataa atggttcttt cgccagggaa gaaaaaggca cctttattat tcctcaaaaa
    27361 gagtgtaata acatagaggt gatattaaag taaaatgggg tagattttga acccctccgg
    27421 gaatctcttt ctaattagga caagggcaga aaaatttagt tctgagtacc gggcagatgt
    27481 tttcaaaaag cgaagtcaaa catagagagg actactatgg tgcaagataa cagaaaaaag
    27541 attaaaaatt atactggagc ttcccccccc tcagcaattc taaactcctg ataggaaaaa
    27601 ataaccggag aaatagaaaa gcggcttaaa aaagcgagac tgctacagag tagctctcgt
    27661 gatctccacc tgacgcaata ttcctttgtg ccacaggcta ttcactttat tgataggaaa
    27721 gcttctactg gcggaggagg cgggactcga acccgcatcg cctttcagct taccggtttt
    27781 caagaccggc tccttgccaa ttgggacact cctcctcgct tattatactt taaaaagggg
    27841 gtgctcttca actcctgacc atagagtgat aagcgtcttc tataaatctc tgcaccttca
    27901 ttccgtcttc tacagtggct ccccgggctt ctcttccggc aagacgcaat aaaaagtcat
    27961 gagcgcaagc cacgtgggca tttatccacc ctaccgggaa tttccccgga agagcaaagg
    28021 gaggaggaaa ttgagagatg cactccagag ctttaaatcc tctatctcct cctaagggag
    28081 aagctttatc ttttaagtcg tagactgcaa gataattagg ttgcatggag ttatagcgga
    28141 tagcgccctt cttcccctct atttctaacc tcagctcatc ggcacttccc gtggatatcc
    28201 ggctactttc tatgctccct tcaccaccac cggctaagtc aaaaagaacc actgaccaat
    28261 catctacttt tatttctctt ccttttcttt cagggataaa agttcttccc caggaagctt
    28321 taaggtgggg ttcttctccc cctaagtaaa agaggaggtc tactaaatga gaccccaaat
    28381 ccagaagcgc tcctcctcca gagagcttcc gatctaagcg ccaagtcagg gggcgctccg
    28441 catccaagta gccagagtga agatataaag cccggaagcg aagaatttct cctaaaaatc
    28501 cttcttgaat tagctcttta gctcgccaga gagccggaac aaaacgattc tggaaaacta
    28561 cccctaaggg aacattctcc tcttttacca ctctctctat tacctcagct ccttctaagt
    28621 tagaggaaag aggtttatcg cagtagatag ctttcttcgc tcgggctgct tcttctacca
    28681 cctctcggtg caaggcatta ggaagagcaa ccaccacgat atcgatgtcc tcttgagcta
    28741 taagctccga aaaagaagaa gtggccaaag ccacaccggt ttcttcttta actttttggg
    28801 ccgtttccgg gcgagaggta gctattcctc gaatttccgg aagataggga agatcgtgat
    28861 agcaataagg agcggaagag aaagccagag catgcacccg accaataaaa ccaaatccca
    28921 ctatgcctat tcctatcttt tccatttatt gagtctcaaa ttggggagtt atagatttta
    28981 accccctgag gtaaggccgg agggcttcgg gaatagttac tgtcccatcc tcttcctgat
    29041 aattttctaa aatggctacc aaggttctcc ctactgccag tcctgagcca tttaaggtgt
    29101 gaacgtatcg cattttacct tccttattgc gaaagcggat attagctctc cgagcctgga
    29161 aatcttcaaa attgctgcag gaggaaatct ctcggtatct attctgggag ggaatccaca
    29221 cttcgatatc gtaagtcttg ctggcagaaa agcctaaatc tccagtgcat aacgccacta
    29281 cccggtaggg aagattaaga atttgcaaca cttcttccgc atctcgaagg agagatccta
    29341 actcttcata ggaattttca ggaagagcga atttcaccag ttctactttg ttgaattggt
    29401 gctgacggat taatcctcgt acgtcttttc cataagagcc tgcttctctc cgaaaacagg
    29461 cgctgtaggc tacataatac agaggaagtt gttcctcccg gagaacttct tcggagtgta
    29521 aattagtaac cgggacttcg gccgtgggaa taagatgaag gtcatcctga cagtgataga
    29581 ggtcttcata aaatttgggc agctgtccgg taccaatcag agatttagta ttaactaaaa
    29641 agggaggaaa tacctcccgg taatggtgtt tttgagtatg gaggtctaac ataaagttaa
    29701 tgagtgctcg ctctaaaaat gccccttctc cttttaatac tgtaaagcga gaagaggcaa
    29761 tttttgctcc ccgctcaaaa tccagaatat ctaatccttc tccaatttcc cagtgaggac
    29821 ggggagtgaa ggaaaaatct cggggttctc cccatcgctt tacttcttga ttatcatttt
    29881 catctttccc caccgggacg gtagaatggg gaatgttggg aatgaagagc agtttctctt
    29941 tccattgttc ttcaatctcc cggagttttt cctcttcctc ctttattttc cctgctattt
    30001 tctctccttc tcggagtgca tcttgagggt cttggccttc tctttttaga gaagctacta
    30061 aacgggaaat ctcgtttctc ttctgacgta aaatatccac ctctcgctgc acagtgcgtc
    30121 ttttttcatc ctcggagaaa agttcctcca ggatttcaga atccattcct cttttttgta
    30181 gagcttcttc tgtggcttcc ttgttttctt ttatccattt ccagtctaac atttttattt
    30241 tcctctcatc ctttaattac tcctaaggga cgggcataaa ctactttttt ggaaattccc
    30301 gctttttcta ccacttctac cacttctccc acatctttgt aagcttcggg aatctcttct
    30361 cgcaaggtgg ctttactttc tgctcgaacc agaacccctc gctctttcaa ctccccggcg
    30421 atgtctcttc ctttagaacg ccggtcggct tcagaacggc tcattacccg acctgctcca
    30481 tggcaggtgg aaccaaaagt ttcttgcata gctaactccg tccccacgag aatataggaa
    30541 ccagttccca tatctccggg aatcaaaact ggttgtccca ctttccggta gtgaggggga
    30601 agaagggggc taccggcgct gaaagcccgg gtagcacctt tgcggtggac acagagtttc
    30661 atcttttttc cttccacttc atgctcttcc catttagcaa tattgtgggc tacgtcatag
    30721 agaatggaca ttcctaaagc aggggcagat ttttgaaata cttgctcaaa tacttctcgt
    30781 acccagtggg taatgagctg acgattagcc caggcaaagt tggcactgca agccatagct
    30841 tcaaaatacc ctctcccttc tggagagtcg cagggggcac aagctaattg acggtcggga
    30901 acgcttatac cataacggct catagctttt tgcatttcct gcagataatc agtgcatacc
    30961 tgatgtccta aacctcggct tccggtatgc accataataa tgacctgtcc agggaaaagt
    31021 ccaaaagctt ctgctaaatc agggcgaaaa acttgctcta ccacttctaa ttctaaaaaa
    31081 tgatttcctg aacctaaact tcctaactgg tctttccccc gttcccgagc tttagtgctc
    31141 accgcctcgg ggttggcgcc ttccatagct cctctctctt ctatgttttc ttcatcttcc
    31201 ccgaagccaa aaccccggct gatgatatag cgcgctcctt tttccataac tttttctagc
    31261 tcggggatag aaaaagagag cttacctttg gagcccactc ccgaggggat atgactaaaa
    31321 agaagggaga gaagcttctc catatggggt tttacctctt cttcggtgag attggttttt
    31381 atcacccgta ctccacagtt gatgtcaaaa cccactcctc ccggggatat aactccccct
    31441 ttttcctcgg tggctgccac cccaccaatg gggaagccat atccccagtg gatgtcaggc
    31501 atagctaaag aacgtcctac aatacccgga aggcaggcta cattagctac ctgctccggg
    31561 gcttggtctt tgcggatggc agaaatcatc ttttcactgg cataaatcat gccatcggtg
    31621 agcattccct ttttgtaagt tctgggaatt aaccaccgcc agtcgtctac tttttgtaac
    31681 ggtcctttcc aggcctcagc catggtataa gtcctccttt tcaaatatca aaataaacct
    31741 gggctcgcca gagctggggc tctacttcct ctacctttac ttcatagtaa gtacaggctt
    31801 tgaccacccg ctgaatatga tgttttttag gatcgagagg ttctccttcc acctgagctt
    31861 taaggtaata ttgtcctaat tcctgaatat taaattgttt taacactact tcctcagctt
    31921 caaaaagata aagaagctca ttgagccagg tgaccagcag gtcttcataa tcacttccct
    31981 ctacctctac ctgggtggta aaccccccgt ccacttcttc tacgctacag atgagagaaa
    32041 acattcccaa agcagcatta gcaaataatt cttctttggt tttcccccag gctactaatc
    32101 ccacatcagc agtatggtct aaaatttcga atttatccat ttttatcctc taaacggcta
    32161 taaagggggt atcccccttt ataatcccct ttcttttttg tcattccggc ataaccccct
    32221 caaagataaa agctga
//
LOCUS       DAOSVE010000008        31455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_985_length_31455_cov_69.610255, whole genome shotgun sequence.
ACCESSION   DAOSVE010000008 DAOSVE010000000
VERSION     DAOSVE010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 31455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31455
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_985_length_31455_cov_69.610255"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..1126)
                     /locus_tag="PK016_01365"
     CDS             complement(155..1126)
                     /locus_tag="PK016_01365"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HXL01762.1"
                     /translation="MKITPLMKGAMVAVRDCMGVRKEEKVTVITDTGKIDIAQGFLFA
                     LHALGVDATLLMMVPRSHHAEEPPPEIGVAMASVDVALLVTTMSLSHTHARMRATEKG
                     VRIASMPGITEDMLTVGAMTADYEKVSKLSWKLTRLLETTNLVEIYTEKGTDVCLSLE
                     SRKPAIPPDDGLYRERGRWGNLPAGEAFIAPVEESVEGVVVIDGSMSPLGLLSEPIKL
                     TIQGGKVINIEGGMEAEELSQFLEQLADPYAYWIGELGIGTNEKARLTGNVLEDEKAL
                     RTVHIALGMNVDMGGKIESKTHNDGIIRNPTVRFDGKLVMDKGVLMV"
     gene            complement(1159..1497)
                     /locus_tag="PK016_01370"
     CDS             complement(1159..1497)
                     /locus_tag="PK016_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXL01763.1"
                     /translation="MEDITGDRDLKRRVMRLRDLIDYGPGSVVSRTIVDKETGTVTLF
                     AFDEGEGLSEHTAPFDALIYILEGEGEVSLSGELFRLVEGEMIIMPSREPHALRAKKR
                     MKMLLVMIKE"
     gene            1626..2486
                     /locus_tag="PK016_01375"
     CDS             1626..2486
                     /locus_tag="PK016_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXL01764.1"
                     /translation="MPIFRILVVDDEESLREMLKEFLEEEGMLVELAQNGREALEKLH
                     SLSFDLVLLDLRMPGISGIEVLQEIKKEEPELPVIVITAYGSVDNAVETLKMGAFDFI
                     TKPFKLEELLNTISRALEVERLKREKQYLLEEMQDKFSFEGVVGQSPQMKEVMRVASL
                     VAKTDATVLIYGESGTGKELLARSIHHQSNRLDKPFVVVNCGAIAETLLESELFGHEK
                     GAFTGAQARKLGKFELADEGTVFLDEIGEMSPAMQVKILRVLQEKNFERVGGTDVIQV
                     AGRCTNYSRY"
     gene            2554..2946
                     /locus_tag="PK016_01380"
     CDS             2554..2946
                     /locus_tag="PK016_01380"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXL01765.1"
                     /translation="MPIELPPLRERKEDLPLLCDFIIEKHTQKLHKKIKEISPQAMRL
                     LKKYHWPGNIRELDNVIERAIILTDDEIIGVEDLHIFEAPQELHWKSLKELEKDYIEE
                     VLEYTGGDVEKATHILGISPEELEKRKF"
     gene            complement(2943..3341)
                     /locus_tag="PK016_01385"
     CDS             complement(2943..3341)
                     /locus_tag="PK016_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HXL01766.1"
                     /translation="MIRYWRGLWQFIKFNLVGIMNTAVDFGVFMILNHYLGLVYTVSQ
                     VISYSCGMVNSYFFNKFWTFQKKEGFTAIEVIKFILVNLCSLGVSLLVLYILQSKWNW
                     QVLPSKVLATGFSVGVNFLGNKFWVFEAAA"
     gene            complement(3338..4297)
                     /locus_tag="PK016_01390"
     CDS             complement(3338..4297)
                     /locus_tag="PK016_01390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXL01767.1"
                     /translation="MKNKIDFSVVIPVYNEEEVLPETYRRIKQVMDSTQGSYELIFVN
                     DGSQDRSREILEDLARKDERVRLINFSRNFGHQIAITAGLDYAEGQAVVVIDADLQDP
                     PEVILAMIEKWKEGYEVVYGKRIKREGESFFKKFTASFFYRLLKRATSIDIPVDTGDF
                     RLVDRKVADVLRLLREKNRFVRGLVSWVGFRQTSVEYVREKRWAGESKYPLRKMLKLA
                     WDGITSFSDKPLKIATYVGIPLSLFSLVLFLIVLFQYLLGKSTLYASSLITTLNLLVS
                     GIILLALGIIGEYTGRIYEEAKGRPLYIVESTMGFKKEGEEKI"
     gene            5191..6459
                     /locus_tag="PK016_01395"
     CDS             5191..6459
                     /locus_tag="PK016_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXL01768.1"
                     /translation="MKKFNKVIFPLIVVTGGILAVFLFSPRFSSSEQQTTGTANIPTV
                     KATRGTIQQTVSELGTLEPQNRVELKSDVSGEVVKIFAWAGEKVQKDQPLLQLDSTKL
                     ETSLQKAKAQLKSAQADLKKLLAKPTEVELRQAEAQLKEAQIAYQSAQKTLAKNQELF
                     KSGGISQEALEQSKNDVELREVLYLSAQAQFRDVKDGADPEDIEKLEAQIEQIEADIE
                     SIESDLEKCTFKSPIDGTLLVVDPEEGDSIISEQRVIAIGDLSTMKVKILINEIDVPE
                     VKKGQKAIVTLDAFPGQQFEGEVTSVAPEGEIVDNIVAFETTIELPNPDNILLSGMTA
                     EAEIIIKSKDNAILIPLEALEEKGGKAWVLVKGPDGQPTQKEVKVGLRNDTQVEIEEG
                     LEENEEVFLSLSSTSSQSNFGPPMGMPGGR"
     gene            6477..7229
                     /locus_tag="PK016_01400"
     CDS             6477..7229
                     /locus_tag="PK016_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL01769.1"
                     /translation="MNQPVIKVEDLWKIYQLGEVQVEALRGVSFEIEGGEFIAIMGPS
                     GSGKSTLMNILGCLDRPTRGRYFLDGQEVSQLNENQLAQVRNKKIGFVFQSFNLLPRL
                     TALQNVELPLIYAGLSLQKRKERAMESLEAVGLEERVRHYPNQLSGGQQQRVAIARAL
                     VNNPSIILADEPTGNLDTRSSEEIMHVLQKLNREGKTIVLVTHERDIASHAHRILHFR
                     DGQLVDNENVEQPLEALAVLKELTEEKVVESV"
     gene            7216..8424
                     /locus_tag="PK016_01405"
     CDS             7216..8424
                     /locus_tag="PK016_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL01770.1"
                     /translation="MNLSETLQSAVSSILSNKLRSFLTMLGIIIGVASVVAMTSLGEG
                     TTEEIIGQIGSLGSNQITVIPGRSRGIPGMAVASRGGNMLEWKHYLQLQDFGFPGIKN
                     IIVENTTSFTVTYGRESVRTRVIGTTPQYPEVRNFNVDIGRFFDQKELDNAERVAVLG
                     RTVAQDLFASPEEALGKKIRAGRITATVIGVMEEKSSGMQDLGEQVFIPITTMQKRLT
                     GSEYIQSITVQTETEEDLDSAVQFLETFFTNRLKDPEKFSVINQQDILDTINQVTGTM
                     TLFLTAITGISLLVGGIGIMNIMLVSVTERTREIGLRKAIGARPFDILIQFVIESSLL
                     SALGGIIGIVLGIVISQVISKFSSYPAIVSVEAIIIAFSISVGVGLFFGIYPARRASQ
                     LDPITALRYE"
     gene            8455..9861
                     /locus_tag="PK016_01410"
     CDS             8455..9861
                     /locus_tag="PK016_01410"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXL01771.1"
                     /translation="MKKSKIFLFPWGVNLVIFFWLLSSFSSGICQAEEIETINLSYVV
                     EKALTNSLEVLNARDTVETAKLNLSSSASQGLSPEVSLSGNWPIINSEDTNELSFGIT
                     IKDAIYLFDESPTEKTAQILLQEAESQLFLKEEEAKAQAITYYLDILNGEKDYSVAQK
                     TVELYQTLLEDQKRKWEEGNASSVDLLEAQENLENAKSLLQEKERELKLARENLNHLM
                     GVPLDSLFLLTPLSAPSTLSKLDYSSLSLLLQERSSEKETLQREKERKEIEKKETQKT
                     TQPQIALVGEYQGEDFQTQLALDREGILSYQIQGGSSSYLSNSSYFSSPESWGVGLEV
                     SWKIWDGKVTSNQLKAADIEISQMEREIEMLPQTLSLKVETAVNQLLQAQSNRDLASL
                     IQQSAQDTYKIMKQQLQIGFITDRELLQSELQYQQAEVNYQKAENNLWQAILNLLKTL
                     EEPIQIEENQIVIPSFLS"
     gene            9902..11017
                     /locus_tag="PK016_01415"
     CDS             9902..11017
                     /locus_tag="PK016_01415"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXL01772.1"
                     /translation="MKVREKILSLILVIYLTGSLFILEGEAQETTALSLQEAVKLGLE
                     KSSEARLAKEKLEEARVTFEEGKATLLLSPSVIQELSLQNTWKLAQRNYEMTLNQHAL
                     SVEEGYYNVLKAQQSAELAQNNLKSAENQYQNIQTKYSLGMVAEIDLLSAELEFDRAK
                     NEIQKSQRALSLAQFRFNQLIGNSESQNYRLSDKLDFQPSEVNLEESLKYALTHRLEI
                     EQAQDNVTLKAKEVEVNSNPYTPPLTLQKSKVQLSQAQINLENTQNSVILEVYQNYST
                     LKDAEEKVPLQAKNSKIAQEKLHIAQAQFDAGLITTVDLLNQQYSTYEAETAYLQAVF
                     DYQVAQATFFTSLGMSLEERPKTWQEKENTEQPIAQE"
     gene            complement(11104..11592)
                     /locus_tag="PK016_01420"
     CDS             complement(11104..11592)
                     /locus_tag="PK016_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXL01773.1"
                     /translation="MARRLLPARREERRVSPTRRLWDLFDLRTDLDEIFDELMESPFF
                     TAPSLVAGFPPVDIAETENEVIVKVEIPGVDPKDLEVHVTEDSLNIKGEAKDEWEEKD
                     VGYYARERYYGTFERTLPLPAKVKQDEVKAQYKDGVLNITLPKVEPSKPKAKKVEIEV
                     GQ"
     gene            complement(12004..12891)
                     /gene="ftcD"
                     /locus_tag="PK016_01425"
     CDS             complement(12004..12891)
                     /gene="ftcD"
                     /locus_tag="PK016_01425"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HXL01774.1"
                     /translation="MIQSAINLSTSVSSLVEKIVKEISILPGLKVADYSSDLDHNRSV
                     ITLLGEEDSLRKAVLKIVEIAEKEIDISKHQGEHPRIGAVDVVPFTPWRGVNMEDCKK
                     LAWKIGEEIAGRFNVPVYFYGEAALREEHRDLSRIRRGGYELLREEIDKVPERYPDLG
                     PAKLHPTLGAVVIGARGPLVAFNVNLKTQDLEIAKKIARKLRGETGGLLCVKAIGVNL
                     KSKGMVQVSMNLLDFRKSTIYQAFELVKLEAKRWGVEVKGGEIIGLLPLEALVDVAGF
                     YLGIPELSVEQVLEYHLCD"
     gene            complement(12901..13416)
                     /locus_tag="PK016_01430"
     CDS             complement(12901..13416)
                     /locus_tag="PK016_01430"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HXL01775.1"
                     /translation="MKALLVIDLLNDFVHPQGVLFVGEKAKEVIAFSQQLIAEYRKEG
                     MPIIYVCDSHRPDDQEFTLFPPHSIEDSWGGKVIEELIPQASDYVILKRRFSAFFGTS
                     LDLLLREKKIEELEIVGVCTNICVLYSSAEARMLNYQVVVYEKGVTSFDLGAHEWALQ
                     EMKNTLGVEVR"
     gene            complement(13603..14253)
                     /locus_tag="PK016_01435"
     CDS             complement(13603..14253)
                     /locus_tag="PK016_01435"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HXL01776.1"
                     /translation="MMEFDSKETIEQRKNMVERQLILRGIKNQRVIEAFLRVPRHLFV
                     PSSHKDLSYQDTPLPIGYGQTISQPYIVALMVELLNPQEDDRVLEVGTGSGYEAAILA
                     ELVKMVYSVERLPELVKRANGILSQLGYRNVVVVEGDGSKGLPEFSPYDKTIVSACSK
                     QIYPAWVEELKEGGMIVLPLEEDMGQNLIRGIKKRGKLVLENHGGCVFVPLVEDEL"
     gene            complement(14253..14534)
                     /locus_tag="PK016_01440"
     CDS             complement(14253..14534)
                     /locus_tag="PK016_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HXL01777.1"
                     /translation="MAEWVQGRLVLRGRVQGVGYRYFVLRKAGEFDVTGLVRNLSTGE
                     VEVIAEGKKDEVEQFFEEVKQGPVSAYVTSYLEEWSPFSGFYSDFRVGY"
     gene            complement(14557..15351)
                     /gene="surE"
                     /locus_tag="PK016_01445"
     CDS             complement(14557..15351)
                     /gene="surE"
                     /locus_tag="PK016_01445"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HXL01778.1"
                     /translation="MKRILITNDDGFESPGIEALVRAFRGVGEVILVAPQEEKSCSSH
                     SITARHPLRIREVEVGGVKGYAVEGTPADTVILALRLFDDGLIDFVISGINRGPNLGF
                     DVFYSGTVAAAMEAAMSGIPSLAVSLVLNNHFNYQLAAEVAVEVFETFEDLLKREKNV
                     VLNLNVPDVPDRENLRGFSITTLGDRFYLTRVREVESQNPGVREFILEEEKREVDFPE
                     NSDYQAVWHSRISITPLRPNLTDFILGEELKGILQNKGLSPIYRLK"
     gene            complement(15348..16739)
                     /locus_tag="PK016_01450"
     CDS             complement(15348..16739)
                     /locus_tag="PK016_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXL01779.1"
                     /translation="MRKNYDVIVIGAGPAGIFTALELSEKSDLQVLVVDKGKNIGRRS
                     CPARENSNICQKCSPCSLLCGWGGAGAFSDGKLTLSQEVGGQLTSYLDGQLFNEVMHY
                     VDSIYLHFGGTDKVYGADPEKVEELRRKAEIAELVLVPSRIRHLGTDRCRDILKNMYD
                     YLSDKVEIKTSLPVERILVKNGKVEGVKLHNGEEVKSKFVVAAPGRVGADWLRQECLR
                     LKLPLENNPVDLGVRVEVPAAMMDEFTDALYELKLIYYTPTFDDRVRTFCMCPKGEVV
                     TEYNDGIITVNGHSYEDRETPNTNFAILVSTHFTEPFREPIAYGRYVARLANMLSGGV
                     IVQRLKDLQLGRRSTTERISKSPVKPTLRGASPGDLSFVLPFRFLQDVQEMLLALDRF
                     IPGINSPHNLLYGVEVKFYSSRVKLNSYLESDVENLFLVGDGAGITRGLIQASASGVV
                     VAREIVRREGGKE"
     gene            complement(16740..17678)
                     /locus_tag="PK016_01455"
     CDS             complement(16740..17678)
                     /locus_tag="PK016_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXL01780.1"
                     /translation="MDSVRVGVIGVGYLGQHHARIYSELPGVELVGVVDIDRDRAKEV
                     AERYSTSAFFDYRDLFGKVDAVSIVVPTVIHRDIAGKFIEEGINILIEKPVTTSLKEA
                     KELMEMANRKKVVLQVGHIERFNSAVVELSKIVDHPIFIDCSRMGPYANRNTDVGVVL
                     DLMIHDIDIVMSIVRDKVVKINAFGYPVFSREEDIANAQLLFSNGCIANLTASRITRK
                     KIRRMEVTQLDSFISIDYLEQELAVYKKTSSSFPYLLMEKPPMQKGEPLRLELEHFIK
                     CVRNGEKPLVGLEEGTNALEVALNILEEIKKKHGDI"
     gene            complement(17704..18804)
                     /locus_tag="PK016_01460"
     CDS             complement(17704..18804)
                     /locus_tag="PK016_01460"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013468199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HXL01781.1"
                     /translation="MINIPLSKPCLGEEEKRAVNEVLDSGFLAQGKNVKKFEEEFAHY
                     VGVKEAIATSSGTAALFVALKALGVKEGDQVITTPFTFVATASSIFQCGGMPVFCDVD
                     EKTFNLDPGKLEDILKKERNIKGIIAVHLYGLPCDLEAITFLTQKYNLFLLEDCAQAH
                     GAKCQGKKVGSWGDMGVFSFYPTKNMTTGEGGMVTTNDPLLAQECRMLINHGSKKRYY
                     HEMLGYNFRLTDIGGALGLVQLTKLEGFNEKRRQNAAFYNRELANLPLISVPYIPSYA
                     LPVFHQYTLRAKEGRDALLQFLRDRGIGCEIYYPLPLHHQPFLRDLYPALSFPVAENL
                     AREVLSLPVHPQLTEEDLNTVVWGVREFFLNH"
     gene            complement(18801..19994)
                     /gene="aroC"
                     /locus_tag="PK016_01465"
     CDS             complement(18801..19994)
                     /gene="aroC"
                     /locus_tag="PK016_01465"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HXL01782.1"
                     /translation="MLEFHTAGESHGPGIITLIRGLPANLEIDIPFINQELSRRQSGF
                     GSGKRMEIEKDEVEILAGVRWGKTLGGPVVFEVKNRDYKNWSTLMDPRGSAPSSYEEI
                     TIPRPGHADLGGMVKYRFSDLRNVIERASARETVGKCLAGAVAKIFLRHFGVKVGGFV
                     ESIGEVSAQEELNWEEKISRARHSPLHTYDPVRENEMIKLIDKVKEEGDTLGGTFVVV
                     AQGVVPGLGSYSEIQERLDSRLAFFLMGIPGIKGVEIGEGFRSSTLRGSQFHDEIFYD
                     SHRSPFPFYRQTFRSGGIEGGISIGDIIWARCAMKPIPTLQRGLMSVDVKEKKPTPAR
                     FERSDICAVPRAVVVGEAMLAWVLATVYREKFGGDSIEEVESHFKDYLTYLESFRSLE
                     DGERQ"
     gene            complement(19969..20319)
                     /locus_tag="PK016_01470"
     CDS             complement(19969..20319)
                     /locus_tag="PK016_01470"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HXL01783.1"
                     /translation="MTVEEHDEICAMTSHLPHILSNCFVSTILSRRKDLPQYAGSSFE
                     DWIRIAGSSPRMWRDIFITNRDKILEAIYLFQKDLDKMTEIIKEGNEEELLSLLQGIA
                     HLKEESIAGISHRR"
     gene            complement(20316..20831)
                     /locus_tag="PK016_01475"
     CDS             complement(20316..20831)
                     /locus_tag="PK016_01475"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HXL01784.1"
                     /translation="MEKLGIAIVGLGLMGGSLGLELASWPQKKFIKGYDIDQRATEKA
                     KELGAVDFISSSLEEMAQGVDLIFLALPVMSVPPVFLELRPYLSSSTRILDLGSSRGW
                     VWREISPALCGLEYAGFHPMGGREKSGIEYAKRGILRDVPILVSPPPVSESGKKLVEE
                     IAHFLGGQVFF"
     gene            complement(20833..21849)
                     /gene="aroF"
                     /locus_tag="PK016_01480"
     CDS             complement(20833..21849)
                     /gene="aroF"
                     /locus_tag="PK016_01480"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HXL01785.1"
                     /translation="MIIVLRSGATQEEINDVVERLKKLGFGAHVSQGVERTIIGAIGD
                     ERRVNLEEKIGVLPFVEKVIPVLAPYRLTSREFKEADTIVKVDNVEIGEGKFVLIAGP
                     CSVESEEQIVETALRVKEAGAKILRGGAFKPRTSPYSFQGLAEEGLKFLAKAREVSGL
                     PIVTEALGVEELPLVVEYADMIQIGARNMQNYRLLEAVGKLRKPVLLKRGMMNTVEEL
                     LMSAEYILAQGNYQVVLCERGIRTFEPSTRNTLDLSCIPLVKMQSHLPILADPSHGTG
                     RSDLVETMSLAALSSGADGLIIEVHPNPVEALSDGPQSLTPEQFHHLMKEIKKLAVVM
                     GKEV"
     gene            complement(21862..22887)
                     /gene="rlmN"
                     /locus_tag="PK016_01485"
     CDS             complement(21862..22887)
                     /gene="rlmN"
                     /locus_tag="PK016_01485"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HXL01786.1"
                     /translation="MEKKSLIGLLPSDICSWLETEGEPVFRARQIFQWIYGKKTDSFL
                     EMSNLPLTLREKLAHTFSLGNSQIEEIQKSKDGTVKFLIRFLPQDTVEVVFIPHRDHR
                     TLCLSTQVGCPAGCTFCATGLSGFRRNLTYQEIVEELWVVEKETGMVANNLVFMGMGE
                     PLLNYENLNQALQVINSPEGFRKGARRMTISTVGIPDKIIKLGKDWPEINLAWSMHAP
                     RDELRDLLIPLNKVYPLGQVLKAIREYLAITHRRVTVEYTLWQEINDRKEDAQEVAKL
                     LKNLLIHVNLIPGNLVSSSAFSPSSPGRVKAFASVLEEKGIEVTIRKPRGQDIQAACG
                     ELYRIYG"
     gene            complement(23098..23728)
                     /locus_tag="PK016_01490"
                     /pseudo
     CDS             complement(23098..23728)
                     /locus_tag="PK016_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067985.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MjaI family restriction endonuclease"
     gene            complement(23733..25010)
                     /locus_tag="PK016_01495"
     CDS             complement(23733..25010)
                     /locus_tag="PK016_01495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011972916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HXL01787.1"
                     /translation="MITNHKIIIGDSREMREVKDEAVHLVITSPPYWQLKDYGAETQI
                     GFNDSYEDYINNLNLVWNECYRVLHKGCRLCINIGDQFARSVYYGRYKVIPIRTEIIK
                     FCETIGFDYMGAIIWQKPTTMNTTGGATVMGSFPYPRNGIIKIDYEFILIFKKPGEPP
                     KVSREIKEKSKLSKEEWNEYFSGHWNFNGEKQTKHLAMFPEELPKRLIKMFSFVGDTV
                     LDPFLGSGTTTLAAKNLERNSVGYEINHEFLPFIQEKIGANEPATEAKFEFVNQNRIT
                     TNWKQKIQKLPYQFHDPVKFDKKIDPRKLKFGSKIDFSESAREKFYSVKKIISPELLI
                     LDNGLKIKLLGIRSKKETMEAALGFLNSKLKNQKVFLRFDNQKYDSDGNLLCYLYLKN
                     KTFINAHLIKNKLVDVDADLDFRYKNKFMSLLS"
     gene            complement(25282..25374)
                     /locus_tag="PK016_01500"
     tRNA            complement(25282..25374)
                     /locus_tag="PK016_01500"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25338..25340),aa:Ser,seq:cga)
     gene            complement(25372..25830)
                     /gene="tadA"
                     /locus_tag="PK016_01505"
                     /pseudo
     CDS             complement(25372..25830)
                     /gene="tadA"
                     /locus_tag="PK016_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007777845.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
     gene            complement(25827..26306)
                     /locus_tag="PK016_01510"
     CDS             complement(25827..26306)
                     /locus_tag="PK016_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HXL01788.1"
                     /translation="MSEETKSGANRFREVLDKVGLSSRIIDFSCSTRSSSEAAQAIGC
                     EVGQIVKSLVFEGVHTHRPFLVLASGGNRVNEKQLEDLVGEPVRKGDAQFVRAKTGFA
                     IGGVPPLGHEEEIETFIDQDLEKHEELWAAAGDPHSVFRISFQELVELTGGRVVSIK"
     gene            27203..28198
                     /locus_tag="PK016_01515"
     CDS             27203..28198
                     /locus_tag="PK016_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01789.1"
                     /translation="MPIKNIWITYSQEIFENQEENYYKTRNELKGKTLTMIEEALREG
                     GWEVNKINVDLPLPRLTEFLSQKVDLIFNLTICVSGIYNQAFLPLLLDANNIPYLGSN
                     SVVHSLCLDRSLSKLCLRGVGIPTPSYFLWSTKEEIPENLDFPLIVKPRFRSYQEIIS
                     EQSVVSSPEELERELQRVFSETKDEVIGEKFLTGREMIVGIWGNGKNVSPLPIMEVNI
                     SRQVPLWDFKTRNNKGYVEDVLCPAPLEEEQKSQIEKMALRIFQELHMRDYATFKLIF
                     SEKEGIPFFFELNSLPSLYYRHSAFPEMCNASGIDYKTMIQKLARIAEERVVNKI"
     gene            28195..29292
                     /locus_tag="PK016_01520"
     CDS             28195..29292
                     /locus_tag="PK016_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HXL01790.1"
                     /translation="MNQELTFAVLGAGHGGQALAAYLSLKGFRVNLFNRTPERINSIK
                     LMGGIQVEGEIQGFAPLTLVTSDIEEAIREAKVIMGVVPATGHRYLAERLSNYLEDGQ
                     IIVLNPGRTGGALEVKQVLKERECQAKVIIAEAQTFIFASRISGPAQVKIFRIKNSVP
                     VAAIPAHRTVEVVQTLRKALPQFVPEDNVLKTSFNNIGAVFHPTITILNAGRIENTHG
                     EFDYYIEGVTPSVALILEEVDKERVKVGESLGIRAITAREWLYSAYDAHGRNLFEAIQ
                     NNSGYYGIKAPTTIFHRYITEDVPASLVPISSLGHMLEVSTPTIDTMIHLACILHGRN
                     YWQEGRTVEKLGLKGLSIKEIREMVVMEEEK"
     gene            29289..30935
                     /locus_tag="PK016_01525"
     CDS             29289..30935
                     /locus_tag="PK016_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HXL01791.1"
                     /translation="MKKILGASLVNCVHVAGIYRFLSLAEQYGYQTYFLGPAISIEKL
                     IEAIKEEKPERVIVGYRLSEEAARNIFQELKEALQKNQLLGKFVLILSCTPSLQDIAQ
                     KTEIFDYMFTGKERFEEIVQSITGEKESSPEEKYPSTLPERIKAKSPFPLLRHHFGLP
                     SLSETIEGVAQISEARVIDVISLGPDQNAQEFFFQPHLMNEEKKGAGGVPLRHPEDLK
                     KIYQASRRGNFPLLRCYSGTNNLVEWAHLLKETINIAWGAVPLMWYSKLDGRSQRSLS
                     VAIRENQEAMRAYASLGVPLEVNESHQWSLRDAPDSVAAATFYLAAYNAKKVGTRYYI
                     AQYMLNTPPGISPQADLAKMLAKSELIQELEEENFAIFTQTRGGLAHFSVDLNVAKGQ
                     LGASTFLGLFLKPQIIHVVGFCEADHLAHPEEVIESAQIVEGVIKDALFGLPQVQIDP
                     VIVENKERVKREAKLVLEAIKKLGEKEEDPWASPQVLTQAVEEGILDAPHLLGNLHAP
                     GKVKTRIIDGFLRIVNERGEIIKEEERLNSILQRLRLESL"
     gene            complement(30908..31270)
                     /locus_tag="PK016_01530"
     CDS             complement(30908..31270)
                     /locus_tag="PK016_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HXL01792.1"
                     /translation="MVEVGSVVEGKVVGITKFGAFVELEDGKRGLVHISEISNHFVKD
                     VSEYLKLNDQVKVKVIGIAPDGKIDLSIKKVNEDRDYEIKLEKSKALFEQKMNRFLKQ
                     SQEKLTDLKHYNDSKRKR"
BASE COUNT         9756 a         7061 c         5536 g         9102 t
ORIGIN      
        1 gcaagttcaa cctgcacttc atgacgagtg agctgtcata cataaagcca taatacacaa
       61 gaagccaaca cgcaggcaca tagatttttc aagggtttta gagtttaaga aggttcaaaa
      121 agaaagagaa actgtcaaat caaaaaccct ctctttagac catcaaaacc cccttatcca
      181 taaccagctt tccatcaaac cttactgtgg gattacgaat tatgccatcg ttatgggttt
      241 tagattctat tttaccaccc atatccacat tcatacccaa agcaatatgc acggtgcgga
      301 gagctttctc gtcttccaga acgttccctg taagacgtgc cttttcatta gtgcctatac
      361 ccagctcccc aatccaataa gcataggggt cagccaattg ttcaagaaac tgcgaaagtt
      421 cttccgcttc catccctcct tctatattta tcacttttcc tccctgaatg gtaagtttaa
      481 tcggttcact caataacccc aaaggtgaca tagagccatc aatcaccact accccctcta
      541 ccgattcctc tactggagca ataaaagctt ctccagcagg taaattaccc catctccccc
      601 gctcccgata cagaccatca tcgggaggaa tagccggctt tctactttcc aaacttaagc
      661 acacatccgt tcccttctcc gtgtatattt ctacaagatt ggtggtttcc aaaagacggg
      721 ttagcttcca gctcagtttt gaaactttct cataatcggc agtcatagct cccacggtaa
      781 gcatatcttc agtaatcccc ggcatgctgg ctatgcgtac acctttttct gtcgctctca
      841 tgcgggcatg agtatggctc aaactcatag tagtaacaag aagcgctacg tctactgaag
      901 ccatagctac tccaatttct ggaggtggct cctcagcatg atgagagcga ggaaccatca
      961 tcaataaagt agcatccact cctaaagcat ggagagcaaa aagaaaacct tgagcaatat
     1021 caattttacc cgtgtcagta atcactgtca ctttctcttc ttttcttact cccatacagt
     1081 cccgaactgc aaccatagct cctttcatca aaggagtgat tttcaattta tctacctcct
     1141 tactttccta tatctctttt actcttttat catcacaaga agcatcttca ttctcttctt
     1201 tgctcgcaaa gcatgaggtt cccgagaagg cataataatc atctctcctt ccaccaggcg
     1261 aaataattct ccagaaaggg aaacctctcc ttctccctcc agtatgtata tcagagcatc
     1321 aaaaggggcg gtgtgttcac ttaacccctc tccctcatcg aaagcaaata aagtaactgt
     1381 acccgtttct ttatccacaa tggttctact tactaccgat cccggtccat aatcgattaa
     1441 atctctcaat ctcattactc gacgcttcag gtccctatct ccagtaatat cttccatggc
     1501 ttacctcttt actttttaac aaagtatagc ataagaactt caccttcttt taatttcttt
     1561 caaaaataag aaaaaagagc aaatatacta ttttttataa tataataata gaagggggtg
     1621 agttcttgcc aatctttaga attttggtcg tcgacgatga agaatcctta agagagatgt
     1681 taaaagaatt tttagaagaa gaagggatgc tggtagagct ggctcaaaat ggacgagaag
     1741 ctctagaaaa attgcattcc ttgagctttg acctggtttt actggactta cgcatgccgg
     1801 ggattagtgg tatagaagtc ttacaagaga ttaaaaaaga agagcctgaa ctaccagtaa
     1861 tagttattac cgcttatggt agcgtagata acgcagtaga aactttgaaa atgggagctt
     1921 ttgactttat aaccaaaccc tttaaattgg aagaactact caatactatc tctcgggctt
     1981 tagaagtaga acgattaaag agagagaaac aatatctctt agaggaaatg caggacaaat
     2041 tttcctttga aggagtggta ggccagtctc ctcagatgaa agaagtaatg cgagtggcat
     2101 cattggtggc aaaaactgat gccaccgtct taatctatgg agaaagtgga acaggaaagg
     2161 aacttttagc gcgctctatc catcatcaga gtaatagact agataaacct tttgtagtag
     2221 taaattgtgg agctattgct gaaactcttt tagaaagcga attgttcggc catgaaaagg
     2281 gggcttttac tggagcacag gccagaaaac tgggtaaatt tgagctggct gatgagggaa
     2341 cagtgtttct ggacgaaatt ggagagatga gcccagcaat gcaggtaaaa atccttcgag
     2401 tgttacagga gaaaaacttt gagcgggtag gaggaaccga cgtaatccag gtagcaggta
     2461 gatgtacgaa ttatagccgc tactaatcgg gatctaaaga aagaagtacg agaaggtaag
     2521 tttcgcgaag acctttatta ccgcctgaat gtagtgccta tcgagcttcc tcctttaaga
     2581 gaacgaaaag aagaccttcc tcttctctgt gattttataa tagaaaaaca tacccaaaaa
     2641 ttacataaga aaatcaagga gatatctcct caagccatgc gccttctcaa gaaatatcac
     2701 tggccaggaa atatccggga attagataac gttatagaac gagccataat tttaactgat
     2761 gatgaaatta ttggggtaga agacttacac atttttgaag ctccccaaga actccactgg
     2821 aaaagcttaa aagagttaga aaaagattat atcgaagaag ttttagaata cactggagga
     2881 gatgtagaga aagctactca tattctgggc atttcccccg aagaactgga aaaaagaaaa
     2941 ttttaggcgg cagcttcgaa cacccagaac ttgttaccca gaaaattaac ccccactgag
     3001 aaaccagtag ccaaaacctt actgggcaaa acttgccagt tccacttact ttgcaaaata
     3061 taaagcacca gaagagaaac tcctaacgaa cataagttaa ctaaaataaa tttaattacc
     3121 tcgatagcag taaatccctc cttcttttga aaagtccaga atttattgaa aaaatagcta
     3181 ttaaccatcc cgcaagaata agaaataacc tgtgatacgg tgtaaaccaa ccccaggtag
     3241 tggttaagaa tcataaaaac tccaaagtct acagcggtat tcattattcc taccagatta
     3301 aacttaataa attgccatag gcctctccag taacgaatca aattttttcc tctccttctt
     3361 ttttaaaacc cattgtgctt tctactatat aaagggggcg acccttagcc tcttcataaa
     3421 ttctacctgt atattctcct ataattccca gagcaagaag gataatccca ctaaccagaa
     3481 ggtttaaggt agttatcaag gaagaagcat aaagagtgct cttacccaag agatattgaa
     3541 aaagaactat cagaaaaagc accagactga aaagagaaag aggaataccc acataagtgg
     3601 ctattttaag cggcttatca gaaaaagaag taattccgtc ccaggctaat ttaagcatct
     3661 tccgcagagg atatttactt tctcccgccc acctcttttc tcttacatat tccacgctgg
     3721 tctgacgaaa acccacccag cttaccaatc cccttacaaa acgattcttc tctcgaagaa
     3781 gtcttaaaac atcagccact tttctgtcta ccaggcggaa atcccccgta tctactggta
     3841 tatctataga ggtagctctc ttcaaaagtc gataaaagaa agaagcagta aactttttaa
     3901 aaaaactttc tccttctcgc tttattcttt ttccataaac tacttcgtat ccttctttcc
     3961 atttttctat catggccaga ataacctctg gtgggtcctg taaatcagcg tcaattacaa
     4021 ctactgcctg tccttctgcg taatccaacc cggcagtgat ggctatctga tggccaaaat
     4081 tacgggagaa attaatcagt ctaactcttt catctttccg ggctaaatcc tctaaaatct
     4141 cccgacttct atcctgactt ccatcattaa caaagataag ctcataacta ccttgagtag
     4201 aatccatcac ctgttttatc cttctataag tctcaggaag aacttcctct tcattgtaaa
     4261 caggaataac caccgaaaaa tcaattttat ttttcatatc catctccttg gttttaactt
     4321 taactttatt atatcctctc aaccgttact ggcaatagga aaaaaagtga tgcccctatc
     4381 acctccttca ccacccttcc ggagtgaaag tgagcgtaag ggtgaaagtt aaattccctc
     4441 cgtgtcgcca ctcaggaagg attcaggcta taagggagaa atcaactctc tataactccc
     4501 aagaaaaaag acaagactaa agagtcaaaa ttaacattga gggaaaacac ttccgcgtgt
     4561 tatcccagaa tgtttatatc accttttccg tcaccccggc aatctttaag ccggggtcta
     4621 tagtggttaa acacttagat tcccaataaa agattttggg aatgacacaa aatggggagt
     4681 cacctccgtg ttcgtcacct aaaaacaaaa aacataagga gcaaagacca ctgagagaga
     4741 gaaagcgtcc ctcagtggat tcccgattgc tgattttggg aatgacaaaa gaggggagaa
     4801 aagagaaatt ccctcagttg tagcaaacct tttatatagc ctgtcataag ttgttatttc
     4861 tattatagta tcccgcaaaa ctggttaaaa tattataagc tcctgaggtt gctatgggaa
     4921 gtaatagacg aacccatcaa ttatgaaacg taactataaa gaaagtgttg cccaacaccc
     4981 tttttttatt ggggattcac atttcataaa ttggtcacaa attgctcact aaaaagaccc
     5041 aaaaaggctt tatgatttcc cccgaaaaag gaaccaaaaa aataaaaggt tagtatttta
     5101 gagatgagta gtttaacaaa gttcaatttt atcagtttat tgaccagcaa gctataatag
     5161 ttaataatca aaaaaggagc aaaaatataa gtgaaaaagt ttaataaagt catttttcct
     5221 ctcattgtag taacaggagg aattttagcc gtttttctct tttctcctcg tttttcctcc
     5281 tcagaacagc aaaccaccgg cacagccaat atacccactg tcaaagcaac cagaggtact
     5341 attcagcaaa ccgtatcaga gctgggaact cttgagcccc agaaccgggt agaattaaaa
     5401 tcagatgtga gcggagaagt agtaaagatt tttgcctggg cgggagaaaa agtgcaaaaa
     5461 gaccagccac tacttcaatt agattctacc aaattagaaa cctcccttca aaaagcaaag
     5521 gcacaattaa agagtgctca ggcagacctc aaaaaactct tagctaaacc tacagaggtg
     5581 gaactaagac aggccgaagc ccagctaaaa gaagcccaaa ttgcctacca atcagcccaa
     5641 aaaactctgg ctaagaatca agaactgttt aagagtggtg gtatttctca ggaagctcta
     5701 gagcaaagta aaaacgatgt agaattaagg gaagttctgt atctttctgc ccaagctcaa
     5761 tttagagatg tcaaagatgg agctgatcca gaagatatag aaaagctgga agctcaaatc
     5821 gagcaaatag aagctgatat agaatctatt gaaagtgatt tagaaaaatg tacttttaaa
     5881 tcacccatcg acggcactct tcttgtagta gatcctgaag aaggagattc aatcatctct
     5941 gaacagagag tgatcgccat aggagacctc tccaccatga aagtaaaaat attgataaat
     6001 gaaatcgatg ttccagaagt gaaaaaagga caaaaggcta tcgttactct cgacgctttc
     6061 cctggccagc aatttgaggg agaagtaacc agtgttgctc cagaaggaga gatagtagat
     6121 aatatagtag cttttgaaac taccatagaa ttacccaacc ccgacaacat tctcctatcc
     6181 gggatgactg ccgaagcgga aattatcatt aaaagtaagg ataatgctat tctcattcct
     6241 ctcgaagctc tagaagaaaa aggaggaaaa gcctgggtct tagtaaaagg ccctgatggt
     6301 caacctaccc aaaaagaagt aaaagtagga ttaagaaatg atacccaggt agaaattgaa
     6361 gaggggttag aagagaacga agaagtattt ctctctcttt cttctacttc ttctcaatct
     6421 aattttggtc ctcctatggg tatgccgggg ggaaggtaaa attagaggtg aaaacaatga
     6481 atcaaccagt aataaaagta gaagacctgt ggaaaattta ccagttagga gaagtgcagg
     6541 tcgaggctct ccggggggta tcttttgaga tagaaggagg ggaattcatc gccatcatgg
     6601 gaccttccgg ttcaggaaaa tctactttga tgaatatctt gggctgctta gaccgtccca
     6661 ccaggggccg ttattttctc gatggccaag aagtatcaca gctcaatgaa aatcagctag
     6721 cccaggttcg aaacaaaaaa ataggtttcg tttttcagag tttcaatctt cttcctcgtt
     6781 taaccgctct tcaaaacgtg gaacttcctt taatttatgc tggactttct ctccaaaaaa
     6841 gaaaagagcg agccatggag tccttagaag cagtaggtct agaggaacga gttcgccact
     6901 atcccaacca gctttctgga ggtcaacaac aacgggtagc tattgcccga gctttagtta
     6961 ataacccttc cattattctc gctgatgaac ccacgggaaa tctggatacc cgctcgagcg
     7021 aagaaataat gcatgttctc cagaaactta atcgggaagg taaaaccata gttttagtta
     7081 ctcacgaacg ggatatcgct tctcacgccc accggattct ccatttcaga gatggacaac
     7141 tagtagacaa cgaaaacgta gaacaacctc tagaagcttt agcagtttta aaagaattaa
     7201 cggaggaaaa agtagttgaa tctgtctgaa actttacaaa gcgctgtttc gagcattcta
     7261 tccaataaac ttcgttcttt tctcactatg ttaggaatta ttattggagt agcttccgta
     7321 gtagctatga ccagtttagg agagggaaca accgaagaaa taataggcca aatcgggagt
     7381 ttaggctcca accagattac cgtcattccc gggagaagcc gaggtatccc aggaatggcg
     7441 gtggcctctc gaggagggaa catgcttgaa tggaaacatt atttacagct tcaggatttc
     7501 ggttttcctg gaattaaaaa tataatagtg gaaaacacta cctctttcac tgtaacttac
     7561 ggtagagaaa gtgtaagaac tagagtaata ggtaccactc ctcaatatcc ggaagttcgc
     7621 aattttaacg tggatatagg gcgttttttt gatcaaaagg agttagacaa tgccgaaagg
     7681 gtagcagtat taggtagaac agtagctcaa gacctctttg cttctcccga ggaagcttta
     7741 ggaaaaaaaa tccgcgcggg aagaataaca gctacagtta tcggggtaat ggaggaaaaa
     7801 agtagcggta tgcaagactt aggagaacag gtatttatcc ccataaccac tatgcaaaag
     7861 aggttaactg gaagcgaata tattcagagt ataactgtcc agacagaaac ggaggaagac
     7921 ttagattctg cagtccagtt tttagaaact tttttcacta atcgactgaa agatccggaa
     7981 aaattctctg tcatcaatca acaagacatt ttagacacta taaaccaggt taccggaact
     8041 atgaccctat ttttaactgc cataaccgga atttctcttc tcgtgggggg tatcggaatt
     8101 atgaacatta tgctggtatc agttacggag cgtactcgag agattggatt gcgaaaagcc
     8161 ataggagctc gaccctttga tattctcatt caatttgtaa ttgaatcctc acttctaagc
     8221 gctctaggag gtataatagg aatagttctg ggcatcgtaa tttcgcaggt aatttctaag
     8281 ttttcttctt atcccgctat cgtatctgta gaggcaataa ttatcgcttt ttccatttcg
     8341 gtaggagtag gccttttctt tggaatttac cctgctcgac gggcaagcca gctagaccct
     8401 attactgccc ttcgctacga ataagttaca atataaaggg gagctgataa aattatgaaa
     8461 aaatctaaaa tttttctctt tccctgggga gtaaatctgg taatcttttt ctggctcctc
     8521 agctctttct cttccgggat ttgtcaagct gaagagatag aaaccataaa tctctcttat
     8581 gtagtagaaa aggctctcac aaattctctg gaggtattaa atgccagaga cacagtggaa
     8641 accgctaagt taaatttatc ttcttctgct tctcaaggtt tatcgccgga ggtctcctta
     8701 tcaggtaatt ggcctataat aaacagtgaa gatacaaatg aactttcctt tggaataacc
     8761 ataaaagatg ctatctatct ctttgatgag tctcccactg agaaaaccgc ccaaattctt
     8821 ttacaggaag ctgaaagcca actttttcta aaggaagaag aagctaaggc tcaagccatt
     8881 acctattatt tagacattct aaacggggaa aaagattata gtgtagccca aaaaactgta
     8941 gaactatacc aaacgcttct tgaagaccaa aaaagaaaat gggaagaggg caacgcttct
     9001 tctgttgact tattagaagc tcaggaaaat ctggaaaacg caaaatcgct gctgcaggaa
     9061 aaggagcgag agcttaaatt agctcgagaa aacttaaatc accttatggg tgtccccctg
     9121 gatagtctat tcttgttaac tcctctttct gctccttcta ctctctcgaa gttagactac
     9181 tcttcactct cgcttttact acaagaaaga agttcagaaa aggaaactct ccagcgagag
     9241 aaagagagga aagaaataga aaagaaagaa acccaaaaaa ccactcagcc tcaaatagct
     9301 ttagtaggag aatatcaagg agaagatttc caaactcagt tggctcttga ccgagaagga
     9361 attctctctt accagattca aggaggaagc tcctcatacc tgagtaacag tagctatttc
     9421 tcttctcccg aaagttgggg agtgggtctt gaagtgagct ggaaaatatg ggatggaaaa
     9481 gttactagca atcagctgaa agcagcagat atagaaatca gtcaaatgga aagagaaata
     9541 gaaatgcttc ctcaaactct aagtctgaaa gtagaaacgg ctgtaaacca actccttcaa
     9601 gctcaaagca accgagacct cgcttctctt atccaacaaa gtgcccagga tacttataag
     9661 attatgaaac aacaactaca gatagggttc atcactgacc gggaacttct acaaagtgag
     9721 cttcaatacc aacaagcaga agttaactac cagaaagcag aaaataattt atggcaggcc
     9781 attttaaatc tccttaaaac cttagaagaa ccaatccaga tagaggaaaa ccagatagta
     9841 attccttctt ttttatctta aaaatagcga gcttaatcac atttgtttag ggggtgagaa
     9901 tttgaaagta agagaaaaga ttcttagttt aatactggta atttacttaa caggttcttt
     9961 atttatccta gaaggagaag cacaagaaac cactgctctt tctctacaag aagcagtaaa
    10021 attaggatta gaaaagagta gcgaagcacg gttagccaaa gagaagttag aggaagcacg
    10081 agtcactttt gaagaaggga aagccacact gctcctttct ccttctgtta tccaggagtt
    10141 gtctcttcaa aatacctgga aattagccca gagaaattat gaaatgacct tgaatcagca
    10201 tgctctctct gtggaagaag gatattataa cgtcctgaaa gctcaacaaa gtgcagaatt
    10261 agcccagaat aatctaaaaa gtgcagaaaa tcaatatcaa aacatccaga ccaagtattc
    10321 tctgggaatg gtagcagaaa ttgatttact atcagctgag ctggaatttg accgagctaa
    10381 aaatgaaatc cagaaatcgc aaagagctct tagcttagct caattccggt tcaatcagct
    10441 aataggaaat tcagaaagtc aaaattatcg gctctccgac aaactggact ttcaaccttc
    10501 agaggtgaat ctggaagaaa gcttaaaata cgcccttact catcgattag aaatcgaaca
    10561 agcccaggac aatgtcactt taaaagcaaa agaagtagaa gtaaatagca atccctatac
    10621 tccacctctt accctccaga aatcaaaagt tcaactttct caagcacaaa taaatctgga
    10681 aaatacccag aattcagtaa ttttagaagt ataccagaat tactctacct tgaaagatgc
    10741 cgaagagaaa gtgcctttac aggctaaaaa ttctaaaatt gcccaagaaa aattgcacat
    10801 tgctcaagct caatttgatg ccggactaat aaccactgta gacctcttaa atcagcaata
    10861 cagtacttat gaagccgaaa ccgcttatct ccaggccgta tttgactatc aagtagctca
    10921 ggccaccttc tttacctctt taggaatgag tttagaagaa agacctaaaa cctggcaaga
    10981 aaaggagaac accgagcagc ctatagcaca agaataaaaa aattattgcg ctggagagaa
    11041 agcgctccag cgcaatagaa aaaacctacc aataaaaaaa cccggagcat gctccgggtt
    11101 tttttattga cctacttcta tttctacttt tttcgcttta ggcttactgg gttccacttt
    11161 aggaagagtg atattcaaaa caccatcttt gtattgagcc ttcacctcat cctgctttac
    11221 cttggcaggt aaaggcaaag tgcgttcaaa agttccataa taacgttccc gagcataata
    11281 gcctacgtct ttctcctccc attcatcctt cgcttcacct ttgatattca aactgtcctc
    11341 agtaacgtgt acctctaagt ctttaggatc cacccctgga atttctactt taactattac
    11401 ttcgttttca gtttctgcta tatctacggg tggaaaacca gcaaccaaag aaggtgcagt
    11461 gaagaaagga gactccatca actcatcaaa aatctcatcc agatcggttc gcaaatcaaa
    11521 caaatcccat aaacgccttg ttggacttac tcttctttct tctctccgag ccggcagtaa
    11581 tcttcttgcc attccatgtc cctccttcat ttatttttca cccctattat aatcagagaa
    11641 agtcaatttg tcaatacttt agtcaaagaa agtcaaaaaa aatttttaga gtagtttaca
    11701 gcattccttt ttggtcatca caatcgcctc attcttgaaa gaagaaggtt acagtgaaga
    11761 tatgtagtag ctctgttttt tctccagtgc tggtatttta attcctccgt cttttcttct
    11821 ttatcttttt atcgtcgcgg gctattcgcg gttgcgatcg gataatggat tactacaata
    11881 tcaagatata gataggaata acaagcactg agcaaaggaa catctccgtg ttcattatta
    11941 attaaaaatg gctagaaaac tacaaaaata cccaataaca aactttttaa ccatcggaaa
    12001 tatttaatca catagatggt attctaaaac ttgctctacc gagagttcag gaatacccag
    12061 ataaaaacca gctacatcta ccagagcctc gagaggtaaa agcccgatta tttctccccc
    12121 ttttacttct actccccacc gtttagcctc cagcttaacc agttcaaaag cctgataaat
    12181 ggtactcttt ctgaaatcca aaaggttcat ggaaacctga accataccct tgctttttaa
    12241 attaacccca atagctttaa cgcagagcaa tcctcccgtc tcccctcgga gtttacgggc
    12301 aatcttttta gctatctcga ggtcctgagt ttttaggttt acattgaaag ctacgagagg
    12361 ccctcgagct ccaatgacca ctgcccctaa ggtagggtga agtttagctg gacccaaatc
    12421 cggatatctc tcaggaactt tatctatttc ctctcttaaa agctcatatc ctccccgacg
    12481 gatacgagaa aggtcccgat gttcttcccg gagagccgct tcaccataaa aataaaccgg
    12541 gacattaaac ctccccgcta tttcttctcc tattttccag gctaattttt tacaatcttc
    12601 catattcact ccccgccagg gagtaaaagg tactacatct actgctccta tccggggatg
    12661 ttctccctga tgtttagata tatctatttc tttttctgct atctctacaa ttttaaggac
    12721 agcttttctc agactgtcct cttctcccag aagtgttatt actgagcgat tatggtccag
    12781 gtctgaggaa taatcagcta cctttagtcc aggaaggata gaaatttctt taactatttt
    12841 ttctacaagg gaagatacag aagtgctcaa attaatagca ctctggatca ctcttttcaa
    12901 ttaccttacc tctactccca gagtgttttt catttcctgg agagcccact catgagcccc
    12961 taagtcaaag gaggtaactc ctttctcgta aaccactacc tgataattaa gcattcgagc
    13021 ttctgctgaa ctatatagaa cacaaatatt ggtacatacc cccactattt ccagttcttc
    13081 tatttttttc tcccgtaaaa gaaggtctaa agaagtacca aagaaggcac tgaatcttct
    13141 ttttaagata acataatcac tggcttgagg gataagctct tctatgactt ttcctcccca
    13201 gctatcctct atactatggg ggggaaagag agtaaactcc tggtcatcag gacggtgaga
    13261 atcacaaaca taaataatag gcatgccttc ctttcgatat tctgcaatta attgttggga
    13321 aaaagctatc acctcttttg ctttttctcc tacaaaaagc actccctgag gatggacaaa
    13381 atcattcaaa aggtctatca cgagtaaagc cttcattttc attcccccta tatagtacaa
    13441 tactatgagg ggtaaacccc ctcataacct cccaaaaaac aaaaaagtaa aagattacta
    13501 aggaaattat agtattcctc agtacatagt gatcttaagt tttgacttcc gacataattt
    13561 actttttaaa gttttagtaa attaataggt tccgcgggaa tactataatt catcctctac
    13621 taaaggtaca aaaacacagc ctccgtgatt ttctaacacc agtttccccc tcttttttat
    13681 tcctcgaatc aagttctgtc ccatatcctc ttccaaaggt aaaacaatca ttcccccctc
    13741 tttcaactct tccacccaag caggataaat ctgtttagaa caagcactta ctattgtttt
    13801 atcgtaagga gaaaattcgg gtagtccctt gcttccatct ccttctacca ctactacatt
    13861 acgataccct aattgcgaga gaataccatt agctcgtttt acgagctcag gaagtctctc
    13921 cacactataa accattttta ctaactcagc aaggatagcc gcctcatacc ctgatccggt
    13981 gcccacctcc agaacccggt catcctcttg cgggtttaaa agttctacca tcaaagccac
    14041 aatataaggt tgagaaatag tttgcccgta tccaatggga agaggagtat cctggtaaga
    14101 gagatcttta tgtgaggaag gaacaaacaa atggcgagga acacgcaaaa aagcttcaat
    14161 tacccgctga tttttgattc ctcgcagaat taattgcctc tccaccatat tctttctctg
    14221 ctctatagtt tccttactat caaactccat cattaataac ccacccgaaa atcagaataa
    14281 aaaccagaaa aaggagacca ctcttctaaa taagaagtaa cataagcact taccggtccc
    14341 tgttttactt cttcaaagaa ttgttctacc tcgtcctttt taccttctgc tataacctct
    14401 acttccccgg tggagagatt cctcaccaat cctgttacat caaattctcc tgctttacgc
    14461 aatacaaaat aacgatagcc cactccttga actctacctc gcaaaactaa tcttccctgt
    14521 acccattcag ccacatcatc tcccccctcc tgatttctac ttgaggcgat agatgggaga
    14581 taaaccttta ttctgtagaa ttccttttag ttcttctccc agaataaaat cagtgagatt
    14641 aggacgcagg ggggttatgg aaatccgaga atgccagacc gcttgataat cagaattttc
    14701 cgggaaatct acctctcttt tttcctcctc cagaataaat tccctaactc ccgggttctg
    14761 gctttctact tctctcaccc gggttaggta aaaacggtct cccaaagtag tgatagaaaa
    14821 accccgaaga ttttctctat caggcacatc aggaacgttc aagttcagca ctacattttt
    14881 ctctcttttt agaaggtctt caaaagtttc aaatacttct accgccacct cagcagctaa
    14941 ttgataattg aagtgattat tcaataccag agaaactgcc agtgagggaa tccctgacat
    15001 agctgcctcc atggccgccg ctactgttcc ggaataaaaa acatcaaacc ctaaattagg
    15061 accacgattt attcccgaaa tcacaaaatc aatgagcccg tcatcaaaaa gacggagagc
    15121 caaaattacc gtatctgctg gcgtcccttc cactgcatat cctttcactc ctcctacttc
    15181 tacttcccta atccgcagag gatggcgagc agtgatactg tggctcgagc agcttttctc
    15241 ttcttgagga gcaaccaata ttacctctcc tactccccta aaagctctaa ccagagcctc
    15301 gatacccggg ctttcgaagc catcatcatt ggtaatcaaa attcttttca ttcctttcct
    15361 ccttccctcc tcactatttc ccgggctacc accacaccgg aagctgaagc ctgaattaaa
    15421 ccccgggtaa ttcctgctcc atctcctaca agaaaaaggt tttccacatc gctttccaga
    15481 taggagttca atttcacccg agaagaataa aacttaactt ccactccata caaaagatta
    15541 tggggagaat taataccagg aataaatctg tccagtgcca ataacatttc ttgtacatcc
    15601 tggagaaaac ggaaaggtaa aacaaaactc aaatctcccg ggcttgctcc tcggagagta
    15661 ggcttcaccg gtgatttgct aattctctca gtagtagaac gccttcctaa ttgtaaatct
    15721 ttcaatctct ggacaatcac tcccccgctc aacatattag ccaatcgagc cacatatctt
    15781 ccataagcaa tgggctcccg aaaaggctca gtgaaatgag tgctcactaa aatggcaaaa
    15841 ttagtattag gagtttcccg atcttcataa ctgtggccat taaccgtgat aataccatca
    15901 ttatattccg tgaccacttc tcctttagga cacatacaaa aagttcggac gcggtcatca
    15961 aaagtgggag tatagtaaat caatttgagt tcatagagag catcggtaaa ttcatccatc
    16021 atagcagcag gaacctccac tcttactccc agatctaccg ggttattttc caacggtaat
    16081 tttaagcgca aacactcttg acgcagccaa tctgcaccca ctctacctgg agctgctact
    16141 acaaatttac tttttacctc ttcaccgtta tgaagcttaa ctccttctac tttcccattc
    16201 ttaactaaaa ttctctccac cggaagagag gtttttattt ctaccttatc actcaagtaa
    16261 tcatacatat tttttaaaat atcccgacac cggtcagttc ctaaatgcct tatccgggaa
    16321 ggaactaaaa ccagctcggc tatttccgct ttcctgcgca gctcttctac tttttctggg
    16381 tctgcaccgt aaactttatc tgttccccca aagtgaaggt aaatagaatc tacatagtgc
    16441 atcacttcat taaacaactg accatcaagg tagctggtaa gttggcctcc cacttcttgt
    16501 gagagagtga gcttcccatc actgaaggct cccgctcctc cccagccaca caaaagagaa
    16561 cagggagaac atttctggca gatattgcta ttctctcgcg ccggacaaga gcgtcttcca
    16621 atattttttc ctttatctac cacaaggact tgcaagtcac ttttttcact caattctaaa
    16681 gcagtaaaga taccagcagg tcctgcgcct atcacaatta catcataatt ttttctcaac
    16741 taaatatcac catgtttctt tttaatttct tctaaaatat ttaaggctac ctctaaggca
    16801 ttggtacctt cttccaagcc tactagtggt ttttcaccat ttctaacaca tttgataaag
    16861 tgctctaact ctaagcggag aggttcacct ttttgcatag gcggtttctc catcaaaaga
    16921 taggggaaag aagaagaagt ttttttatac accgccagct cctgttctag atagtcgata
    16981 gaaatgaaag agtctaattg agtcacttcc atcctgcgga tttttttgcg agtaatcctg
    17041 ctggcagtga gattagctat acatccattg gaaaaaagga gctgggcatt ggcaatatct
    17101 tcttcccggg aaaaaactgg atagccaaaa gcattgattt taaccacttt atcccttact
    17161 atactcatta caatatctat atcatgaatc atcaaatcca gaactactcc cacatcggta
    17221 ttacggttag cataaggacc catccgggaa caatcgataa aaatagggtg gtctactatc
    17281 ttggaaagtt ccaccaccgc tgaattaaag cgctcaatat gccctacctg gagaaccact
    17341 ttcttcctgt tagccatctc catcaattct ttagcttctt tcaaagaagt ggtcactggc
    17401 ttttctatca aaatatttat accttcttca atgaatttgc ctgcgatatc ccggtgaata
    17461 accgtaggaa ctactatgct tactgcatcc actttaccaa aaaggtctcg ataatcaaaa
    17521 aaagctgaag tggagtaacg ctctgctacc tctttagctc tgtctctatc aatatccacc
    17581 actcctacca attccacacc gggaagctca gaataaatcc gggcatgatg ctgacctaaa
    17641 tagcccactc ctattactcc cactcgaact gaatccaaaa ttaccacctc gagtaataat
    17701 aagctaatga tttagaaaaa attcccgcac tccccaaact acggtattca aatcttcctc
    17761 agttaattga ggatgcactg gcaaggataa aacttccctg gccagatttt ctgctacagg
    17821 aaaggataaa gcgggataca aatcccgcaa aaagggctgg tgatggagag gcaaaggata
    17881 atagatttca catcctattc ccctatcccg caaaaactgg agcaatgcat ctctcccttc
    17941 ttttgcccgc aaagtatact ggtgaaaaac gggtaaagcg taagaaggaa tgtagggaac
    18001 acttataaga gggagattag ccagctcgcg attataaaaa gcggcatttt gacgcctctt
    18061 ttcattaaac ccctccaatt tagtaagttg aaccaaacct aatgctcctc ctatatcagt
    18121 aagacggaaa ttataaccta acatttcgtg ataatatctt tttttactcc catggtttat
    18181 aagcatacgg cattcttgag ctaagagagg gtcattggta gtaaccattc ctccttctcc
    18241 ggtagtcata tttttagtgg gataaaagct gaaaactccc atatctcccc agcttcctac
    18301 ttttttaccc tgacactttg ctccatgtgc ttgagcacaa tcctctaaga gaaaaagatt
    18361 atatttttga gttaaaaaag ttatcgcctc taaatcacag gggagtccat aaagatggac
    18421 agctattatt cccttaatat ttctctcttt tttaagaata tcctcaagct tccccgggtc
    18481 aaggttaaag gttttttcat ccacatcaca aaaaaccggc atccctccac actggaatat
    18541 agaacttgcc gtagctacaa aggtaaaagg agtagtgata acctggtctc cctccttcac
    18601 tcctaaagct tttaaagcta caaataaagc agcagtgcca ctacttgtag ctatagcttc
    18661 ttttactccc acataatgag caaattcttc ttcgaatttt ttaacatttt taccctgagc
    18721 taaaaatccc gaatctaaaa cttcatttac tgctcttttt tcttcctccc ccagacaggg
    18781 tttactcaaa ggtatattaa tcattgtctt tctccatcct caagagaacg aaaactttct
    18841 aaataagtaa gatagtcttt aaaatgactt tctacttcct ctatagaatc accaccaaat
    18901 ttttcccggt aaacagtggc caaaacccag gctaacattg cctctcccac gaccactgcc
    18961 cgggggacag cacagatatc ggagcgttca aaacgagcag gagtgggttt tttttccttt
    19021 acatccacac tcattaagcc ccgctggaga gtaggaatag gcttcatagc gcatctcgcc
    19081 caaataatat ctcctattga aattcctccc tctattcctc cactccggaa agtctggcgg
    19141 taaaaaggga aagggctacg atgagaatca taaaaaatct catcatgaaa ttggcttccc
    19201 cgaagagtgg aagagcgaaa accctcccct atttctactc ctttaatccc tgggatgccc
    19261 atcaaaaaga aagctaatcg gctatccaat cgctcttgaa tctcactata gctccccaga
    19321 ccaggaacca caccttgagc taccacgaca aaagtacccc ctaaagtatc tccctcttct
    19381 tttaccttgt ctataagctt tatcatttca ttttctctta cagggtcata agtatggaga
    19441 ggagagtgtc tcgcccgaga tattttttct tcccagttca gctcttcttg agcactaact
    19501 tctcctatac tttccacaaa tcctcctact tttactccaa agtggcggag gaaaatttta
    19561 gccactgctc cggctaaaca ttttcccact gtctctcgag cgctggctct ttcaataaca
    19621 tttctcaagt cggaaaaacg atactttacc atccctccca aatcggcatg gccaggacga
    19681 ggaatagtaa tctcttcata agaagaagga gcgctacctc gagggtccat taaagtagac
    19741 caatttttat aatctctatt tttaacttca aaaaccaccg gacctcccaa agtttttccc
    19801 caccgcactc cggctaaaat ctctacttca tctttctcta tctccatacg ttttcctgaa
    19861 ccaaaaccac tttggcggcg ggaaagttct tgattgataa aaggaatatc tatttccaga
    19921 ttagcaggaa gtcctctgat gagggtaata atacccggac catgagattc accggcggtg
    19981 tgaaattcca gcaatgcttt cctccttaag atgtgctatc ccctgaagca gagataataa
    20041 ttcttcttca ttaccttctt ttataatttc tgtcatttta tccaggtctt tttgaaaaag
    20101 ataaattgct tccaatattt tatctcgatt ggtaataaaa atatccctcc acatccgggg
    20161 agatgaacca gctattctta tccaatcttc gaaagaagaa ccagcatatt ggggcaagtc
    20221 ctttctgcga gatagaattg ttgaaacaaa acaattagaa agaatatggg gaaggtggct
    20281 ggtcatagca catatttcat cgtgctcttc aacggtcaaa aaaaaacctg tcctcctaaa
    20341 aaatgagcta tttcttccac taacttcttg ccactttctg atactggagg aggagaaacc
    20401 agaattggca catctctcaa gatacccctc tttgcatatt ctattccact tttctcccta
    20461 cctcccatag ggtgaaagcc tgcatactct aaaccacaaa gagcgggaga tatttctctc
    20521 catacccacc ctctactgct tcctaaatct aaaattcgag tagaagaaga aagataagga
    20581 cgaagttcca aaaagactgg aggtacgctc ataaccggaa gagccaaaaa aattaaatcc
    20641 actccttgag ccatttcttc taaagatgaa gagataaaat ccactgctcc cagttctttt
    20701 gctttttctg tcgctctttg gtctatatca tagcctttga taaacttttt ttgaggccag
    20761 ctggctaatt ccaaacctaa ggagcctccc attaatccca gaccaacaat agcaatacct
    20821 aacttctcca aatcagacct cctttcccat taccactgcc agttttttta tctctttcat
    20881 caagtgatga aattgttcag gagtaagaga ttgagggcca tcagataatg cttcaacagg
    20941 gttggggtga acctcaatta tgagaccatc agctcccgag gataacgcag ccaagctcat
    21001 ggtttctacc aggtcgcttc ttcctgtacc atggctgggg tcagctaaaa tggggagatg
    21061 gctctgcatc tttactaaag gaatgcaact caggtctaaa gtattacgag tagagggctc
    21121 aaaagttctt atgcctcttt cacataaaac tacctgataa tttccctgag ccagaatata
    21181 ttcagcagac attaaaagtt cttctacagt gttcatcatg cctcgcttaa gaaggacagg
    21241 tttacgcaat ttccccactg cctcgagaag acgataattc tgcatatttc gagcccctat
    21301 ttgaatcata tctgcatatt caacaactaa gggcaactcc tccactccca gagcttcagt
    21361 aactatgggt aacccactta cttctcgagc cttagctaaa aattttaatc cctcttccgc
    21421 taacccctga aagctataag gggaagtacg aggtttaaaa gctccccctc gcagaatttt
    21481 tgctcccgcc tcttttaccc ggagagcagt ctctacgatt tgctcctctg actctaccga
    21541 acaaggacca gctatcaata cgaattttcc ttcccctatt tccacattat ccacttttac
    21601 tatggtgtcg gcctctttaa attcccggct ggtcaatcta taaggagcaa gaaccggtat
    21661 taccttttct acaaaaggta atactccaat tttttcttct aaattaactc tccgctcatc
    21721 accaatagca ccaataatgg ttcgctctac tccctgagaa acatgagccc caaaaccgag
    21781 ctttttcaac ctttctacta catcattaat ctcttcttga gttgccccac tccgcaatac
    21841 gataatcatc gcttttctct cctaaccata aatacggtaa agttctccac aagccgcctg
    21901 gatatcttgt cctcgcggtt tccttatcgt tacttctatc cccttttcct ctaaaactga
    21961 ggcaaaggct tttaccctcc ccggtgaaga cggagaaaaa gcagaagaag aaactaaatt
    22021 accaggaata agattaacat gaatcaataa attcttcaat aatttcgcta cttcctgagc
    22081 atcttctttc ctgtcattta tctcctgcca gagagtatat tctacagtaa ctcgccggtg
    22141 agtaatagcc agatactctc ttatcgcctt caaaacctgt cctaagggat aaactttatt
    22201 aagaggtatc aataaatcgc gtaattcatc ccggggagca tgcatggacc aggccagatt
    22261 aatttccggc caatcttttc ccaattttat tattttatcc ggtatcccaa ccgtagagat
    22321 agtcattctc cttgctccct tgcgaaaacc ctctggagag tttattactt gaagtgcttg
    22381 atttaaattc tcatagttaa gcaaaggctc tcccattccc ataaatacca ggttattagc
    22441 caccatccct gtttcttttt ctaccaccca tagttcttct actatttcct gataggtaag
    22501 atttcgccgg aatcccgata aaccagtagc acaaaaagtg cagcctgctg gacagcccac
    22561 ctgggtggaa aggcaaagag ttcggtggtc tcgatggggg atgaatacta cttccactgt
    22621 atcctgagga agaaaacgga ttaaaaattt tactgttccg tccttcgatt tctgaatttc
    22681 ctcgatttga gagttaccca aagaaaaagt atgagccagc ttttcccgta aggtaagagg
    22741 aaggttgctc atctcaagaa aggagtctgt tttctttccg tatatccact ggaaaatttg
    22801 tcgagctcga aaaacaggct ctccctcagt ttccagccag gagcaaatat cagaaggcaa
    22861 aagcccaatt aaacttttct tttccatttt tttataatta ataacctcgg gtttaaaacc
    22921 cagaaaaatg gtttacaaca atacttattc aagaatccac caatatgatt atcaagcata
    22981 actgttaccc acctggtaaa aacctttctt tttttattca tcaggaattt gaaaaacgtc
    23041 aattatatac tcaatagctt cttttattga ttgttctttc ttgtctccca aagttaacta
    23101 taaattttct aatctttcaa tttcttgctt tatttcttct attacctctg gattataaat
    23161 tctctttttg tcgctctctt tgatagaaat aacataaaaa aacctttcca ccatatttag
    23221 cagtaaattt tttgtgagtt acctcttgat ttttctttca tttataattt gagttataaa
    23281 agcatatcct gctggtttaa tttgcaaacc aatatactta tcctttattt taataaaaaa
    23341 tccacattaa ataatctatc ccattcatct gaagctggct caatttttac acctaaaatg
    23401 ttttgaagct gttcataaat tgttttcttt tctgtcatat atctatcaaa agtccggtta
    23461 atgactagat taataacata atttatgcaa tcttcttccg taatatcatc tatttctgcc
    23521 cttaaaactt cgcttatctt cacataaagc ttcctaccta tttcaataag atgttccttg
    23581 gggtaaacat ttgcaaaata gtattcttcc caatcttcca ctgttttggg agaacatttt
    23641 attatttcat ccgcaactgc tccaaccatt cttttcttct ggagacccaa cggtttattg
    23701 cactatttaa gatccactct tttgccatag tcttatgata ataaactcat gaatttattt
    23761 ttataacgaa aatccagatc agcatcaaca tccaccaatt tattctttat taaatgagca
    23821 ttaataaaag tcttattttt taaataaaga taacataata aattaccatc actatcatat
    23881 ttctgattat caaaccttaa aaagaccttc tgattcttta atttagaatt taaaaaacct
    23941 aatgctgcct ccattgtttc ttttttagat ctgataccaa gtaatttaat ttttaatcca
    24001 ttatctaaaa tcaatagttc aggacttata atctttttta ctgaataaaa tttctctctt
    24061 gctgattctg aaaaatcaat ttttgagcca aactttaatt ttcgggggtc tattttttta
    24121 tcaaatttaa ccggatcatg aaattgataa ggtaattttt gaattttttg tttccagtta
    24181 gtggttattc tgttctgatt tacgaattca aactttgctt cagtagcggg ttcattagct
    24241 ccgatttttt cttgaataaa aggtaaaaat tcatgattta tttcatatcc aactgaatta
    24301 cgctccagat ttttggctgc caatgtcgtt gttccacttc ctaaaaatgg gtctaaaacg
    24361 gtgtctccaa caaaactgaa catttttatc aatcttttag gtaattcttc agggaacatc
    24421 gccaaatgtt tggtttgttt ttctccgttg aaattccaat gtcctgaaaa atattcattc
    24481 cattcctctt tgcttaattt tgatttttcc ttgatttctc ttgaaacctt tggaggttca
    24541 ccaggctttt taaaaatcag aataaattca taatctatct taattattcc atttcttggg
    24601 tatggaaaac ttcccatcac tgttgctcca ccggtagtgt tcattgtcgt tggcttttgc
    24661 caaataatag cacccatata atcaaacccg attgtctcac aaaatttaat aatttccgtc
    24721 cggataggaa ttactttata tctaccataa taaacagagc gagcaaactg atcaccgata
    24781 tttatacaca atcgacaccc cttatgtagc acacgataac attcattcca caccaaattt
    24841 aaattattaa tataatcttc gtaactgtca ttaaaaccaa tttgtgtttc tgcaccataa
    24901 tcttttaatt gccagtaggg tggagaggta ataacaagat gaaccgcttc atcttttacc
    24961 tctctcatct ctcttgaatc tcctataatg attttgtgat tagtaatcat actttattaa
    25021 tccctcaaat attaactcta atgtcatcgt aatatttttc acactacccc cgagaactcc
    25081 tcaataaccc cgtttgaatc agggtggaag aatagttaag taaatatatg agtgatatat
    25141 tgtttactat gtaagaatac cagatatttt aaagctaaac aagggaaaca atgcaaaacc
    25201 tgcttttgac ccgtattatt acaggacaaa tacaaaaacc ggagttagca aaccccccta
    25261 aatcctccag atactaattg ttggcggaga gggtgggatt tgaacccacg agaccgctca
    25321 gcacggccta ctcgatttcg agtcgagcgc cttcgtcccc tcggccacct ctcctctttt
    25381 caacttaaaa tatttcttga gaatctggct acatttattt tctaatattc ccccttctat
    25441 ttctacatag tgagggaaaa gttttctatc aaaaacatta actacacttc ccgccgctcc
    25501 ccacttgggg tctcgagctc caaaaaaaac tttcttaatc cgggattgaa ttatagctcc
    25561 agcacacata agacagggct ccagagtaac aaaaaggata gccccttcca gacgccagct
    25621 atttaaaacc cgagaagctt gattaatagc cacaatctca gcgtgagcag taacctcctg
    25681 tcgtttttct ctctggttat gagagcgagc tatgacctcc cctcctaaaa ccacacaagc
    25741 acccactggg acctcgtctt cccagaaagc ctgttgagcc tcctcccagg caatttccat
    25801 aaatctttct tttacaatat ctccattcat ttaatcgaga ctactcgtcc tccagttaat
    25861 tctactaatt cttgaaaaga aattctgaac acgctgtgag ggtcaccagc agcggcccag
    25921 agctcttcat gcttttctaa atcctggtca atgaaagttt ctatttcttc ctcatgacct
    25981 agaggaggaa ctcctccgat agcaaaacca gtttttgccc tcacaaactg agcatctcct
    26041 ttgcgaacag gctctcctac cagatcctct agttgctttt cattaacccg attccccccg
    26101 cttgccaaaa ccaaaaaggg gcgatgagta tgcactcctt caaaaaccaa agatttaaca
    26161 atctgcccta cctcacatcc aatcgcttga gctgcctcac ttgaacttcg ggtagagcaa
    26221 gaaaagtcta ttatccgtga ggataatcct actttatcta acacttctct aaaccggttt
    26281 gctcctgact ttgtttcctc ggacatagca tttctctcct tcatacattt tacctttctc
    26341 ttcctacttt gcaagaccct caggagttct gtaataaaat gtaggaggcg ttcctcttca
    26401 cctatcttat ctgcgcatca tcgcttttct ctgtaacaat aggtttttca aaagagaggt
    26461 tgttccctcc ataactcctc acaaagacag aaaaaagttt cataagaagt tttctctcta
    26521 tggtttttta gtgtcatttt caaaatcttt aaccgggaat ctaaatacca ccgaaagagg
    26581 aaaacactct ttcggtggta tttgctctgt ttgttgtttt ttgaagggga ttataagctc
    26641 atcattaccc atatctatct ggtctggaca caaggcagcg gtttttgccg ggcgtggcga
    26701 tcccgagttt tcaacacaat gtagcttgtc ttttatgttt tttgtatttt ttgccttttg
    26761 ggagttggca agggggcgta cctctactga gggaagcttt tctcctcagt ggtttttgtc
    26821 ttttgtctac gccgtaggct gtctttgtag ttaatcataa atagtgttca gaaagcaatt
    26881 gtgggacatg atgagactat aaggggattt atccccttat agtcgataga aacattcaga
    26941 aatgacgggc aatgggatga tagggacatt caggggcagg ctaaaaacgg ctcgaaagtt
    27001 accggggtga cgaaaaatag gattgtggga atgagtaaag aaatatccag gtgacagata
    27061 agagagttat tttccccgta acgatattgt ttttttcttt ctggaaaggt agaaaagaga
    27121 gaccgcagac cacctgccgt gaaatttgca tcaagaaacc aatttatgct attctacttc
    27181 agtaaaaagg taaaaggaag tgatgcctat taaaaacatc tggatcacct acagtcagga
    27241 aatcttcgaa aatcaagaag aaaactacta taaaactaga aatgagctca agggtaaaac
    27301 tttaactatg atagaagaag cgttacgaga aggaggatgg gaggtaaata aaataaatgt
    27361 agacttgccc ttgcctcggc taaccgagtt tctatctcaa aaggtagact tgatatttaa
    27421 cctcaccatc tgcgtttcag gtatctataa ccaggctttt cttcctcttc ttttagatgc
    27481 taataacata ccttatctgg ggtctaattc tgtagtccat tctctttgtc tagaccgctc
    27541 cttaagtaaa ctctgcttaa gaggagtagg tatacctacc ccttcttatt ttttatggtc
    27601 taccaaagaa gagataccag aaaatctgga ttttcctctc attgtaaaac cccgttttcg
    27661 ctcttaccag gaaataatta gtgagcaatc agtggtctcc agtccagaag aattagaaag
    27721 agaattgcaa cgggtttttt ccgaaaccaa agatgaagta ataggggaaa aattcttaac
    27781 tggaagagaa atgatagtag gaatatgggg taatggaaaa aatgtctccc ctttacctat
    27841 tatggaggtt aacatctctc gccaggttcc tctctgggat tttaaaacca ggaataacaa
    27901 aggatatgta gaagatgtat tatgtcctgc tcctcttgag gaagagcaga aaagccagat
    27961 agaaaaaatg gccctccgga ttttccagga attgcatatg cgagattatg ccacctttaa
    28021 attaatattc tccgagaaag aaggaatccc ttttttcttt gaacttaatt ctcttccctc
    28081 tctctattac cggcacagcg cctttccaga aatgtgtaac gccagtggta tagattataa
    28141 aactatgata cagaaattag cccggatagc cgaagaaaga gtggttaata aaatatgaat
    28201 caagaattga cctttgctgt actgggagca ggtcatggag gacaggcttt agctgcctac
    28261 ctgagtttaa aaggatttcg agttaatctt tttaatcgca ctccagagcg aataaattct
    28321 ataaaactta tgggaggaat acaggtagag ggagaaatac aaggctttgc tcctctgact
    28381 ctagtcactt ctgatataga agaagccatt cgagaagcaa aagtaattat gggagtggta
    28441 cctgccaccg gccatcgtta cttagctgag agactaagta attatttaga agacggtcaa
    28501 attatagttc ttaaccctgg acgcactgga ggagcattag aggtaaaaca agtattaaaa
    28561 gaaagagagt gccaggctaa agtaataata gctgaagcac agacttttat tttcgcgagc
    28621 aggatttctg gcccagctca ggtaaaaatt ttcagaatta aaaatagtgt tccggtagct
    28681 gccattcctg ctcatcgaac agtggaagta gtacaaactt taagaaaagc tctccctcaa
    28741 tttgttcctg aagataacgt tttaaaaacc agcttcaata acattggtgc ggtttttcat
    28801 cctactataa ctatacttaa cgccggtcgt atcgaaaaca ctcatggaga atttgactac
    28861 tacatagaag gagttactcc ttcagtagct ttaattttag aagaagtgga taaagaaaga
    28921 gtaaaagtgg gagaatctct gggaataaga gcaattactg ctcgagaatg gctttattct
    28981 gcttatgatg ctcatggccg caatcttttt gaagctattc agaataattc aggatactat
    29041 ggaattaaag cccctactac tattttccac cgctacatta cagaagatgt tccagccagc
    29101 cttgttccca tctcctccct ggggcatatg ttggaagtct ccactcccac aattgatact
    29161 atgattcacc tagcctgtat tcttcatgga agaaattact ggcaagaggg aagaacagta
    29221 gaaaaactgg ggctgaaagg cttgagcatt aaagaaatcc gagaaatggt agtgatggag
    29281 gaagaaaaat gaaaaaaatt ttaggagcat cgttggttaa ttgtgttcat gtagctggta
    29341 tatatcgttt tttgtctttg gctgaacaat atggctacca aacctatttt ttaggtccgg
    29401 ccatctcgat agaaaaacta atagaagcaa taaaagaaga aaaaccagaa agagtaatag
    29461 tgggctaccg tttgagcgaa gaggcagccc ggaatatatt ccaggaactc aaagaggctt
    29521 tacaaaaaaa tcaactactg ggcaaatttg ttctcatttt aagctgtact ccctccttac
    29581 aggatattgc ccagaaaacg gagatttttg attacatgtt caccggtaaa gagcgcttcg
    29641 aagaaatagt acagagtata accggggaaa aagaaagttc tccggaagaa aaatatcctt
    29701 ccacacttcc cgagagaata aaagctaaaa gccccttccc cctcttgagg catcacttcg
    29761 ggctaccttc tctatctgaa accatagagg gagtagctca aattagcgag gctcgggtta
    29821 tagacgttat ttctttaggt ccagaccaga acgctcagga attctttttc cagcctcacc
    29881 tgatgaatga ggaaaaaaaa ggagcagggg gtgttccttt acgtcatcct gaggatttaa
    29941 aaaaaatata ccaagcctct cgccggggaa acttccctct tttacgctgc tatagtggca
    30001 ccaataattt agtggaatgg gctcatcttt tgaaagaaac tattaacatt gcctggggag
    30061 cagtgcctct tatgtggtac agcaagttag atggtcgctc tcaaaggagc ctctccgtgg
    30121 ccattcggga aaatcaagaa gccatgagag cctatgcctc tttaggagtt cctcttgaag
    30181 taaatgagtc tcatcaatgg agtttacgag atgctcctga tagtgtggca gcagccacct
    30241 tctatcttgc tgcttacaat gctaaaaaag ttggaactcg ctattatatt gctcaatata
    30301 tgctcaacac cccaccaggt atctcccccc aagctgattt agccaaaatg ctagctaaat
    30361 cggaattaat acaagagctg gaagaagaaa attttgctat tttcactcaa acccgaggag
    30421 gattagctca tttttccgta gacttaaacg tagccaaagg acaactggga gcttctactt
    30481 tcctgggttt attccttaaa ccccaaatca tacacgtagt aggtttttgt gaagccgacc
    30541 atttagccca tcctgaagaa gtaatagaaa gcgctcaaat tgtagaggga gttataaaag
    30601 atgctctctt cgggcttcct caagtccaga ttgatcctgt tattgttgaa aataaagaga
    30661 gggtaaaaag agaagctaaa ttagttttag aagcgatcaa aaaattagga gaaaaggaag
    30721 aagacccctg ggcctctccc caggtgctga cccaagcggt agaagaggga atattagacg
    30781 caccccattt gctgggcaat cttcatgcgc caggaaaggt taaaacacgc atcatagacg
    30841 gattcttaag aatagtaaac gaaagaggag aaataattaa ggaagaagaa aggcttaatt
    30901 ccatccttca gcgtttgcgt ttagaatcat tataatgctt caagtcagtg agtttttcct
    30961 gactttgttt caggaaacgg ttcatttttt gctcaaagag agctttactt ttttctaatt
    31021 ttatttcgta gtctctatct tcattaactt ttttgatgga aaggtcaatt ttcccatcag
    31081 gagcaatacc aatgactttt acctttacct gatcattcaa ctttagatat tcgctgacat
    31141 ccttcacaaa atgattggag atctctgata tgtgtactaa acctcttttc ccatcttcta
    31201 actctacaaa tgctccaaac ttagtaatac ccaccacttt gccttccaca acgctaccaa
    31261 cttcaaccat taacttctaa ctatcctcct ttataaaaat tgagcttatt atacaaacga
    31321 caccctgagg tgtcaagaat atatctgtta ttgacttttt catctttttg tatttttttc
    31381 acagtcggct tcttaagcat tctttctcta tggcctttat ttcttttgtt ttttaagttt
    31441 tcttaatctt gcgtc
//
LOCUS       DAOSVE010000009        30864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_1009_length_30864_cov_70.585284, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000009 DAOSVE010000000
VERSION     DAOSVE010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 30864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30864
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1009_length_30864_cov_70.585284"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            373..1695
                     /locus_tag="PK016_01535"
     CDS             373..1695
                     /locus_tag="PK016_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01793.1"
                     /translation="MKANLIPLYFKSGKNDEFLAQVQNLKKLLAEEAIIGEPLALGSP
                     LPQEADAIIFPQVLGDAYKQIKDIEALRLPILVVTSEFGTVDMWDWEIVSYLKSKGLK
                     VFAPYNLELTKKAIKSLALKREMKSTRFLVFQDNPGEGMQASIFKRFYWWEEECTNLI
                     QEKFGISIIKKSFKKLAEEAKQVPDAEVEELWKRWNLPLEGVSPKALHSALKIYRVLK
                     QEVEQDPSIKGMGINCLNESFYSDTTPCLAWNMLFEEKGIIWACEADTLSLLTQFIIH
                     KSLGAPIMMSNIYPFLMGMAALKHEKISSFPEVDQPENHLLVVHCGYLGVLPKSFSSD
                     WKLKPRVLEIVDENATAIDARLPEGEVTLAKLDPTLSKLLIVEGYLEGYVQYPGSDCR
                     NGAIIRIPDGHLLMDKLYSHHDIIMIGHRGVELKSMAYALDIEVEWVK"
     gene            complement(1810..3153)
                     /locus_tag="PK016_01540"
     CDS             complement(1810..3153)
                     /locus_tag="PK016_01540"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HXL01794.1"
                     /translation="MKKEDFPPDFLWGVATASYQIEGAYNEDGKGESIWDRFAHTPGT
                     IADGSTGDVACDHYHRCKEDIELMKELNINSYRFSVSWPRIFPCGRDNVNKKGLDFYK
                     SLIEELLKNNIKPMLTLYHWDLPQHLQFHGGWVNPETSKAFVDYAVTLFEEFNGLVDL
                     WITFNEPWCISFLSHQMGKHAPGIEDYPTALKVAHNLLTAHGETVKVFKERGFSGKIG
                     IVLNLSPVHSFTDSPEDREAANFMDCYKNRWFLDPLFKGTYPEELFQVFVNNFGDFTR
                     GYQDISGASEKMDFLGINYYSRDVVQYDGGEILKAKSVTVEAERTDMGWEIYPEGLRE
                     IVSKVHKEYGAIPLYITENGAALKDELKNGEVHDWQRIEFLKNHFKSAYQAIQSGVDL
                     RGYFIWTLMDDFEWECGYTKKFGLFHVDYDNNLQRIWKDSAKWLREFLGNKVSLE"
     gene            complement(3211..3939)
                     /locus_tag="PK016_01545"
     CDS             complement(3211..3939)
                     /locus_tag="PK016_01545"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXL01795.1"
                     /translation="MAQHVYTAYVTGADRGLGLALTRVLLENQFKVYAGQYLPDWPEL
                     GKLKTQYPEHLVIVPLDTSKEESVEEAAKTIKSDTSYLDIVINNAAVYLESRSGNIFE
                     ELCYEDMLKTFNINSLGPLRVTHSTIDWILKGERKLVINISSEAASIGNCWRKKEFGY
                     AMSKAALNMQSAILQNHLKEYGVKVLAIHPGYMKTYMLGKKNLSADVEPEESALKIYQ
                     LFISQKQDLEGPVFYDYQGKQLPW"
     gene            complement(3939..4361)
                     /locus_tag="PK016_01550"
     CDS             complement(3939..4361)
                     /locus_tag="PK016_01550"
                     /inference="COORDINATES: protein motif:HMM:NF024093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXL01796.1"
                     /translation="MIKAFDHVTITVSNLKRSVDFYQRILGFKMMGMLEQNGGDFKIV
                     YLDAKGATLELFYFKHGGQPLPEVKDPDIGIKHIGFRVDNIDEVVKKLKKADVNFTLE
                     PFDATGGVRIAFFKDPDGILIELVQGELKLLPYKEETI"
     gene            complement(4377..5363)
                     /locus_tag="PK016_01555"
     CDS             complement(4377..5363)
                     /locus_tag="PK016_01555"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXL01797.1"
                     /translation="MRVAVIGLGFGELHVRSLLKIENVQSIVICDKDENKLREISHKY
                     SIEKWSTSYEEILSDDSIEVITLAVPHYLHKPMVEKALRSNKHVYCEKPLTVKFSDAE
                     ELVRMAEERHLALTVGFNMRYYKQYQQAFSMLDAGSTGRVFLVECFARANARNLQGFR
                     LKKEEAGGGCLIDSGAHRFDLLRWLFGSVKSIYTRAGTYVIDKMEGEDTALVSMLFQS
                     GVIGSLICSWGTCVPPWDEGMCIYGDKGSISIWDHDLSFCFRNCAGEEEIQKYRISYE
                     DSITLSLRDFLEQVERGNVKVDRVKALAHLQLIEASYKSWEKNLPVEINNPI"
     gene            complement(5379..6212)
                     /locus_tag="PK016_01560"
     CDS             complement(5379..6212)
                     /locus_tag="PK016_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXL01798.1"
                     /translation="MNIKLKIKAGDIVRYIFLFIVFVVTIFPLVWMVINSFKNRSEIF
                     IGPLWPEKFMLDNYKFVIENLDIGKSFMNTTIVTAVSIFLILVVSVMAGYAFAKIKFP
                     GRNAIFLALIASLTIPPQVTLIPLFLMLKEMSLLDSLTGLIASYTGLGVAFSIFLMRA
                     FFVTIPDALRESAIIDGASEFTAFWKVVLPLAKPGIATVAIFQFVSIWNEFMYAATFI
                     LSPEKRTLQPTIRSLVGRYSTDWGSLCAGMVIAVIPILVTFIILQKQFIKGLTAGAIK
                     G"
     gene            complement(6202..7110)
                     /locus_tag="PK016_01565"
     CDS             complement(6202..7110)
                     /locus_tag="PK016_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXL01799.1"
                     /translation="MRKRRSLEKSQHIFGYLMIIPALLFFVIFVIYPSLDAIGLSLSF
                     WHGYGERVFVGLQNYINLLHDPVFWLALKNNIIFVIATTILQTLIPMVLAVMLVRNNF
                     LNTFFRVSFFVPNIISFIITGSLWAMIFEPNFGLLNTILQNLGLETWKQLWLADPKWA
                     FPCIVFVSLWQSMGFYLIIFYAGLQRIPDEVYEAASVDGADSLRRFLFITVPLLRPVT
                     TVVVIINVIGGFKVFDLIWAMTTGGPNHATEVMATYLYTLAFGSVGTGTPRMGYAAAI
                     GIMILIFSVLASVIQLRIREADTLEY"
     gene            complement(7177..8433)
                     /locus_tag="PK016_01570"
     CDS             complement(7177..8433)
                     /locus_tag="PK016_01570"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXL01800.1"
                     /translation="MARKVKVTIATLILSGLLLVAAAGAQEAVTITMWTWFDDFQTAF
                     PKIVDKWNSEHPEIRVEQQVFSLNEYLQKLQAAVAGESEPDILAPHVMVRSLGEGGVT
                     IDFNEWLDEEFLSRFAPGTIRQFTWDGHLYSLPWTAQTFGIFYNKDIFEKMGLNIPET
                     WDDLIEVSHTIRGMDSSIQPLPFGNRDKWLGCDFFLPLITQVTDDPTLLIELDRRTDP
                     EREVSWNSEPVIEALKLVKKLVEGEVFAPGINGIANEQALTMFYMGKGAMFYSGSWLP
                     VTIDREAPQGFNWDVFETPAYAPGKRHWTGNESGANLAVSARSKHIEEALEFLKFMYS
                     PEVYSPAMVEARGMPATMEALQGLEDPAIKKMASWLEDGAPHILYGEGSWDAVANAVQ
                     GLMGGQLTPEEAAAQIESDVQRVIGR"
     gene            complement(8706..9377)
                     /locus_tag="PK016_01575"
     CDS             complement(8706..9377)
                     /locus_tag="PK016_01575"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HXL01801.1"
                     /translation="MKQKLLMIGDNEKAPYHPLREIEPVIESILQPEFEVCFSEDYEE
                     ILSDYSRLEQFELVVSYLDRWEEPLNDLQTGNLLRYFSVGKGLVAIHCGISLQARHEF
                     KHIIGARFIKHPPLAKLEFFAESKKHPISEGINVFEIEDEPYLFEFDNFSERELFLHY
                     KNSNKEIFPAGWITYFGPAKIIYLMPGHSCKSFENKIYQQLLLQSIRFTIESKGGDEA
                     KGIEK"
     gene            complement(9443..10291)
                     /locus_tag="PK016_01580"
     CDS             complement(9443..10291)
                     /locus_tag="PK016_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXL01802.1"
                     /translation="MARKFFNPYIKETALHLIIVVLLVLGAIIVASPFFWMVTSSFKT
                     GQEMFMFPPTLLPRKWTLESYYKLFASVPFLRYYLNSILITIAYTIIGLFISSLTGFA
                     LAKYRFRGREIIFSTVVLTLMIPMYAFVIPLYAFIAKTGWMDTYQAVIFPFAANGLTI
                     FFMRQGMLAIPDALLDAARIDGCTEFGIFWNIALPISKPSLVVCATLLATYAWNDFLW
                     PLVVLRSSEHFTVPIALANFVGLYAVDYASLMAGSVLATLPILVLLISLQRYFLAGLL
                     TGAVKQ"
     gene            complement(10300..11229)
                     /locus_tag="PK016_01585"
     CDS             complement(10300..11229)
                     /locus_tag="PK016_01585"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXL01803.1"
                     /translation="MQAKVNSCGDIPAGIKHKISQHYYRFQSKWAPYIFVAPFLIFFA
                     LFFLNPVIEAFHMSFYKFEGLGSAHFVGVENYINLTKDARFFRALLNSTYFAIAWLLI
                     LIPISLLLAIAINALGKRSGTFFQVLYYSPSVVSLVVVAIVFRMIYDRSFGFANLVLS
                     WIGISPLAWLEQPNLALLSIILLSAWRYIGVDALYFLAGLRSLPSEIFESAAIDGANR
                     FQTFMYITVPLLRPITIFVIVITVISSYQLFTEPFLLTGGGPGDATLTPALYLYQRGF
                     SFLDLGYAASMGLVLGGIIFILSFVQRKLLKEE"
     gene            complement(11241..12524)
                     /locus_tag="PK016_01590"
     CDS             complement(11241..12524)
                     /locus_tag="PK016_01590"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXL01804.1"
                     /translation="MKKKGWKWGIVLLFTVLFCVQVAGAQTEIKVWSFGSQTAWLEEV
                     AKSFEAANTDIKVKVEDYPYQQMHDNLLIAVQTGVGAPNLATIEISQVAKFYRGDKVN
                     LLDLTSYLEESGYLDKFYESRLAPFTFHGALYGIPFDLDPAVFFYRSDIYQENGIDVE
                     NIQTWDEFVDVGLKFKEQGKYVLQLGNGAASTAEWGHFWMWLIQAGGGVFDEEANVIL
                     DNDIAKKILSYYSDAVVKHEIANTSPQDVGTPPWWAPYRASQVVSDFGPDWYVGFLKA
                     NVPEQEGLWRAKSLPAWQPGGYTSSTWGGTMLSIVPSNSETDKAALAFLSYAMLENKD
                     NLVSFYLKTGMIPPRKDLVDAEVFDAPDSYFGGQTIGRLLLDQAPQVPSFYLAPTWPE
                     ATDLLNRKVLLGACLGDDPAYLLEEAANELRQLGK"
     gene            complement(12630..13694)
                     /locus_tag="PK016_01595"
     CDS             complement(12630..13694)
                     /locus_tag="PK016_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007951927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HXL01805.1"
                     /translation="MVPEKMRAAIVEKPGEIKIRDVPVPQISPEEVLVKVKFCGICGT
                     DYSIYTGKYSKEFLPLIPGHEFSGEIVQVGEKVKNLKIGQRVTADINLSCGHCFYCTR
                     GQSLICPEFRQLGIHMDGAFAEYLKVPAKQIHILPDEVGFEAGAFIEPISCAIHAAKA
                     MEVSFGSSVAVIGDGTLGILHTQIAKLRAAAPVILIGKHKERLEAARKMGVDYLIDIS
                     KEDVVRMVKELTEGRGADFVIEAVGTVSTYETALKIVRPGGRIAAFGITNQEDEMKLR
                     PYDFVLGERSLVGSCAGVGNDWSDAIALLRFGRINPQPLFSLKVPLEALEEALLEGKE
                     NRRLLKIFVSPELTEKEVIN"
     gene            complement(13712..15019)
                     /locus_tag="PK016_01600"
     CDS             complement(13712..15019)
                     /locus_tag="PK016_01600"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HXL01806.1"
                     /translation="MAKKIDISTSLAGVKMRSPFGVSPHNLDKPWFPGKKAAELFMKY
                     IEAGAGFIYIPALVPGEPTRAEKELDFPELFKTQNYVGRWMKVTLDSQHKEAIAHIYT
                     AKNLFNYLSWGKELVSELRPSLPPDVPIIAQVLVHDTNPAKWADQTKRVVDELQPDII
                     ELNTGCPVGAMGHLDAQNLPPEAKWGMMMGAAPEVFLPVLEACVKATELPVGFKLTPE
                     VGYPRMLYLTEEAARAGAKFVVTTHKYFAVPPPDIWNGGKGKYPGVSDNANVLSDIGG
                     PALRFSMYKATALISKNIPGIETFAGGGITSPEHIVEAIMLGARACQALTGIVLNGID
                     FIGRANRWLQRYMEKCGYEKIDDFKGLGLQYLKSSTEVEFLYHRAEIDEDKCTRCGKC
                     AASYCPAISMKDGKPFVNESFCSSCAMCTVICPEDAISIVPRG"
     gene            complement(15042..16016)
                     /locus_tag="PK016_01605"
     CDS             complement(15042..16016)
                     /locus_tag="PK016_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006443634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HXL01807.1"
                     /translation="MKLGFFTANFSDRPLEEVLDKICKLGYEAVEIPAFSGSPHLDIE
                     KVIDNPSYAKDIREMVEDRGLFISALSNHPEGQLVLGPYGEDTDAIFKGSPEEKIKYG
                     MERMIKTAQAANALEVPVVCGFIGCENFGRFFPWPYSKGWAEMEQKFVERWGKILDEF
                     EKYGVKFAHEPHPNELVYDIYTAQRSLELLGEKKAWGFNFDPANLVYLGIDVVNFIQI
                     LGQRIYHVHAKDGEIVEHNVKRDGLIPTGPWTRIDRGFRFRIPGWGSVPWKRVITELA
                     MIGYDYVLSYEHEDVTMSREDGATKTADFLKPLLIKAPYEGRKDILFQ"
     gene            complement(16045..17484)
                     /locus_tag="PK016_01610"
     CDS             complement(16045..17484)
                     /locus_tag="PK016_01610"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HXL01808.1"
                     /translation="MVLHYSSFINGIEQEGEGEIKVTDPSNGKVFATVSKVTANQIEE
                     AIEGAYQAFQSWSQKLASERAHILFRAADKVREIAKDIGRLLAQEQGKAFPDAEKEVL
                     GAADCLEYYGSLAMNIVGEIPPNSASHLQSLAIRQPVGVVFAVAAWNYPVSLISWKVA
                     PALAAGCTVIVKPSRETPLSTIEFVKAINEASLPEGALNVIIGDNTLISQKIFSDPRI
                     RLVALTGSTETGKEFIKASAPTVKKLILELGGHSPFIVFADANFEKAVKDGLKRAFRN
                     AGQICNSVNRILVEGKIKDSYIKAFVEATQKLRLGGAFEEPAPDLGPLVNQAGIERIK
                     DFIEDAQVKGGKVLWGGKKPDDPRLQEGFFFEPTVITEVTPDMKIMNEEPFGPVVAID
                     SFQSIEEAIEKANSVRYGLVAYLYTQDMKKAFRVAERLEWGSVAINNISPDSLYAPYP
                     GWKESGLGVELGHQGLEEYLEWKHIKWEI"
     gene            complement(17484..18983)
                     /locus_tag="PK016_01615"
     CDS             complement(17484..18983)
                     /locus_tag="PK016_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HXL01809.1"
                     /translation="MVSKDRGVLVIDIGTESVRAALVDEKGKVRQIEGRSPSFFSPHP
                     GWAEQSPEEWWELSSQCVRSLNLKDTRMEIVAVGVTAQMHAVVPVDNKGRVLLKQVPI
                     WCDKRSQKICEEVSQEIPLQEQLNKAANLIIPNWTGPKMKWIKEYIPEVYNQTSVFLT
                     AKDYLNFKLTGSYFTDFSEASGSFLFSWKEKDWSDELLSLFDLDQEKLPPIIPSVQVV
                     GQVQEEVAHFLDIPSGIPVICGAGDMLCLLLGGGMVEEGKSCDVAGTAADVSIFSSYP
                     LISPKLMNLHHGVDGWISFGILDSGGGSLRWFRDAFYQDEGKTVSYSEIDKEAQTIDS
                     AGLLFFPYLLGERLMGSPHARGVFLGILPHHQRKHFARAVMEGVSFDLKMSLEEIEKL
                     RQEKISHLTAIGGGAKSNLWCQIKADIYEKEIWALEETEGGIMGAAMLSFSSALKRPV
                     SQLAKEWIKVRKKFLPEPDRLRAYRKEYQQFKNFHDSLQDIFSQYGKEN"
     gene            complement(18987..19370)
                     /locus_tag="PK016_01620"
     CDS             complement(18987..19370)
                     /locus_tag="PK016_01620"
                     /inference="COORDINATES: protein motif:HMM:NF018288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXL01810.1"
                     /translation="MRYYKKNLLDAVPEEGPSAFAELLKGSDSKVLRDTLMLLTKDNT
                     ESGRIDVGFTVIYPGCNTKGHSHSPKEEVYFITEGEGIMIIDEEEFEIQKDDVVYIPF
                     GKFHRTINSSPLPLSYIWITVEKEK"
     gene            complement(19388..20134)
                     /locus_tag="PK016_01625"
     CDS             complement(19388..20134)
                     /locus_tag="PK016_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXL01811.1"
                     /translation="MDKQVDKEETSFYFKIYKSLKEEIENGTYKVGDLLPSEIELGER
                     FGASRTTIRNALGELEKEGLVSRRRGKGTMVQEPKTTQNLNYISSFTETLKEKGIEVT
                     TGSLTVELITAPPKIASLLNLESDEKVYLVQRTRIANNIPIAFMSNYLLARVIPGLEF
                     KKEILRQEGLYQLLEREYGLQLHRAVETIEAYLSGPLESDLLQIPQRIPLFHTVRTTY
                     LEDETPFEVVISVIRADKYEYKVYLEGRPH"
     gene            20393..21037
                     /locus_tag="PK016_01630"
     CDS             20393..21037
                     /locus_tag="PK016_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HXL01812.1"
                     /translation="MQLKSRQEVTQFLEDSKIIAIIRAQEAQSLTEAVTALREGGINC
                     IEVTMTTPGALKLLEEVSGKVEGVLFGAGTVLDPETARLCILSGAQFLVTPSLNFSVM
                     EMAHRYDIPIIPGALTPTEILSAWEKGADVVKVFPAGSLGPSYIKELKGPFPQIKLCP
                     TGGVNLDNLSTFLKAGASCAGVGGALVKNEFIREKKWEELKKLAKQFKEATQNG"
     gene            complement(21034..21372)
                     /locus_tag="PK016_01635"
     CDS             complement(21034..21372)
                     /locus_tag="PK016_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HXL01813.1"
                     /translation="MANGEKVRYIGVDTPELHHPQKEVEYYAREAYEANRRLVEGKRV
                     RLELDVEERDRYGRILAYVYVDDLMVNEWLVANGYARVATFPPNVKYAERFLQLEREA
                     RQAKVGLWAD"
     gene            complement(21516..22454)
                     /locus_tag="PK016_01640"
     CDS             complement(21516..22454)
                     /locus_tag="PK016_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HXL01814.1"
                     /translation="MSQVVVLARSFSQGSDEPRELLNKAGLEVVFKKNIDPEKEEKVA
                     ELIGEAEAVIVGVDRIGEVVFSRCSNLRVVSKHGVGVDNIDLESAKRHKVVVANAPGT
                     NSQSVADMALLLILASARGLPTLLEQVKNKVWSSPFLGVELEDKVLGIVGWGRIGKEV
                     GKRGQGFGMKVIYYDPLVEDKEFASVSLEDLFKRADFISLHAPLTKDTEKMVGEKLLS
                     LMKEEAFLINTARGELIDEEALYRFLQEKRIKGAALDVLSCEPPFESPLLSLPNVIVT
                     PHVSAHTKEANIKMGRIAALNVIRVLQGEEPLYRVV"
     gene            complement(22451..23008)
                     /locus_tag="PK016_01645"
     CDS             complement(22451..23008)
                     /locus_tag="PK016_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003871013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HXL01815.1"
                     /translation="MVWIILLIVVVLVIYLIVLYNRLVVYKNRVDNSWSQIEVQLRRR
                     YDLIPNLVETVKGYASHEKEVLENVTRARQMAMGAQTPQERGEAEEGITRALRQLFAV
                     VEAYPTLQANENFQSLQKELSETEDKIAYARQFYNDTVYKYNTMIEKFPANIFARSLG
                     FSRREYFEAGEGRGPVQVNFREEEK"
     gene            complement(23022..24863)
                     /locus_tag="PK016_01650"
     CDS             complement(23022..24863)
                     /locus_tag="PK016_01650"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HXL01816.1"
                     /translation="MKKFVLSFWIFILVLFLLPSFAFSKSYFHPSINQSFVLQENGDV
                     EVEEERYFSFSGSFSWAQLTILLRGVKDIDFSGVWSIETGEPLPFQVEEEAHSKSLKW
                     FYQAEDEIKGFRIKYTLRQVVKRYRDVAEFYWKVLEDEHAEVEDLRVSFTLPQASPEL
                     LKLFVHAEVEPGEIVFSPDYKRVDFYLSSIPSDTFVEARLLLEPQIFPLLSPLDDNRY
                     EEILQEEERLINEGPQVSRWTIFFSFLLVGGGILYIILFFYYYFRYGKEPKIGYEREY
                     EQEPPRDVSPSLLGSILSQQEASPQVMSRAFLATILDLARRGYLKVEEKEEKKFIGKK
                     NYLNFTFTERGISENKNDALLPLEQEVLELLHRISKEGKSVSTLDIEEWGSEFRGDKS
                     KFSSFIEQWGEKARSFWEEHFFPLLDPLSEIKKTQFFTLGMIYSGIAIFYVFLALTSG
                     NSFWGAFLVIPFIPLALFFAFLSLKSLSRWSKEGALEFKRWQAFRRFISDFSLMKEAP
                     PLLLHIWDRYLVYAVVLGVAEELLKNLKLYAQERGEALVVPTWYHPVGGTFTLASLDG
                     LSQLDNFSNRMENLAHLRQAVSTSTSVGGGFSHGGGGGGGGGSSRAG"
     gene            24930..25655
                     /locus_tag="PK016_01655"
     CDS             24930..25655
                     /locus_tag="PK016_01655"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HXL01817.1"
                     /translation="MFFLQKFWGAFLTLPGILVTIPLFLFLWGLIKKKINWSLLFLAL
                     LSYFFSTGAGLYLLPREAPSRDATATPQAIVVLGGGRVSSFLRTYQAFLLWEKEQIPL
                     VLSGGKIEQGSKNSEAEIMKELLISWKVEEEDIYLEPVSRTTWENALYTTEILREKGW
                     QTFYLVTSEIHLKRACFSFHHLYPEAHIIPVSGDPIYNFSAFSWMDFLPSGEAFSAWV
                     EIIHEYIGYLFYRVVYGTDSLRR"
     gene            26006..26437
                     /locus_tag="PK016_01660"
     CDS             26006..26437
                     /locus_tag="PK016_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01818.1"
                     /translation="MTPEDRLMKVTIIIGIALIVLTALAIYLIYGAYLFKEEWPWMRF
                     SIIMAVLTCLWSYAFWFDRTTMDKDISDRLWYVVVFVPFDVLLMYPGHKALGLYVDHH
                     FEIFIIIIMIIHGFGFYFIQKEIQKYLKQKYLNNRSNNYET"
     gene            26737..27048
                     /locus_tag="PK016_01665"
     CDS             26737..27048
                     /locus_tag="PK016_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01819.1"
                     /translation="MSEIAESDIEELQAKISKEAITRNYQCGKTIHEELREVVLAVER
                     LRVAKSFRPSEDVKAIDFNSKGIRSVSCFFHFIIWLECTPDLLDISRMVLSHSVLLVL
                     F"
     gene            complement(27160..27618)
                     /locus_tag="PK016_01670"
     CDS             complement(27160..27618)
                     /locus_tag="PK016_01670"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HXL01820.1"
                     /translation="MKKTLIVGLIACIILIGSSLALRQEDFTELVKTGSLEEVKKAIE
                     AGANVNARDENGMTPLMHAAGANQNPEVIKVLLEAGAEVNARDEDGWTPLMQAACFNQ
                     NPEVIKVLLEAGADGKIRCKEGKTAFDYAKENKHLRNTEVYWLLKDAQYL"
     gene            complement(27959..28555)
                     /locus_tag="PK016_01675"
     CDS             complement(27959..28555)
                     /locus_tag="PK016_01675"
                     /inference="COORDINATES: protein motif:HMM:NF019191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HXL01821.1"
                     /translation="MVFLKQYGKTAYFYLVILLMAIFLAGCGATPEPIVTPTPTHSPT
                     PTPEPTVFNETPSQRNAVETARIYLSFMAFSYDGLVKQLVYDGFSTEDARYAADNCGA
                     NWYEQAVKAAKSYLSFMAFSYNGLIDQLEHDGFTYDEAKYGADNCGADWYEQAVRAAR
                     SYLSIFPFSYDRLIRQLEYDGFTTEQAEYAASQSGLTP"
     gene            29133..29573
                     /locus_tag="PK016_01680"
     CDS             29133..29573
                     /locus_tag="PK016_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01822.1"
                     /translation="MKRKNFLIVLLLVLAVYLSGCSGGIVTPNAYEVGGDFYVCENLL
                     RGFYTAISNRNYAQALSYCKPGGSSFEFVNDIWNLDQQYPTTYITCQVYNVYDFSYIG
                     QSRISLHYDFSDTAHDIYGGTYNTNYHYGATALFEKVNGEWKLF"
     gene            29639..30238
                     /locus_tag="PK016_01685"
     CDS             29639..30238
                     /locus_tag="PK016_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HXL01823.1"
                     /translation="MYYGKMENKKKHFRIPEVLTPEEQEKFLKTFNKKAKTGMRNYAM
                     CRLMLDAGLRCGEVLNLKVSDVDFQTGKIHVKQGKGNKDRIVWLNEEALDLLREWRER
                     KPESELLFTTLQGKPINPRYVRDMVKRKAKQAGIDKDVHPHTLRHSFATDLYRESKNL
                     RLVQKALGHSSISTTEIYTHIVDEELEKEMKFFRSKEVA"
BASE COUNT         9536 a         6797 c         5554 g         8977 t
ORIGIN      
        1 cattcaggaa taacggcaag aagacaccct caccctaacc ctctcccttc gagggcgagg
       61 gaaaaaagat gtaaagctct cccttcaagg gcgagggaag gaatatgtgg ggttcttcca
      121 ttgaaggcga gggaagaaaa agaaagggga ttcataaggg ggaattcttt cccccttata
      181 ctgaggaaga cgttttttct tcctcagttg tttttgcctc tttaagtctt ttgtcttttt
      241 tttgcttttt gggggattat aaaggggttc tcccctttat agttcggggt tatgaggggg
      301 cagcgccccc tcatagtctt tgtaatggat tttgcggtta actataaaac ctatagatag
      361 gagggctgtt tggtgaaagc taatcttatt cctttatact ttaagtcagg taaaaatgac
      421 gagtttcttg cacaggtaca aaatttgaaa aaacttcttg ctgaagaagc tataatcggt
      481 gaaccgttag cattaggctc tcccctgccc caggaagccg atgcgatcat ttttccccag
      541 gtcttaggag atgcttataa acagataaaa gatatagaag ctcttcggct tccaatctta
      601 gtggttactt cagagtttgg aacagttgat atgtgggact gggaaattgt aagttacctc
      661 aaatcaaaag gactcaaagt gtttgctcct tataacttag agcttaccaa aaaggccatt
      721 aaaagcctcg ccttaaaaag agaaatgaaa agtaccagat tcttagtttt ccaggataat
      781 ccaggtgagg gaatgcaagc cagtatattc aagcgctttt actggtggga agaagagtgt
      841 accaacctca ttcaggaaaa attcggaata tctataataa aaaagagctt taagaaatta
      901 gccgaagaag ccaagcaagt tccagatgct gaagtggaag aattgtggaa aagatggaac
      961 cttcccttag aaggtgtttc tccaaaagca cttcatagcg ctttaaaaat ctaccgggtc
     1021 ttgaagcaag aagttgaaca agacccttca ataaaaggga tgggaattaa ctgtcttaac
     1081 gagtcttttt actctgacac caccccttgc ctggcctgga atatgctgtt cgaagaaaag
     1141 ggaattatat gggcctgtga agcagatact ctgtctttac tcacccagtt cataattcat
     1201 aaatctctgg gcgcaccaat tatgatgagt aacatttatc ctttcttgat gggtatggca
     1261 gcactaaagc atgagaaaat aagcagcttt cctgaagttg atcaaccaga aaaccatctc
     1321 ttggttgtcc attgcggcta tcttggtgtg ttgcctaaat ctttttccag tgattggaag
     1381 ttaaaaccca gagtccttga aattgtagat gaaaatgcca cagctattga tgcccggcta
     1441 ccagaaggcg aagtaactct ggctaaactc gaccctactc tctctaaatt gctgatagtt
     1501 gagggatatc ttgagggata tgttcaatat ccaggttcag actgtagaaa tggagctatc
     1561 atcagaattc ccgacggtca tctcttgatg gataaattat attcccacca cgacataatt
     1621 atgattggcc accggggagt ggagttgaaa agcatggcct atgccctaga catagaagta
     1681 gagtgggtaa aataagaaaa gagtaaatat aagtagatac cttccaccac ttagtaatgg
     1741 aaggtatcta cttgggaaat taacttaacg aaaaactttc ttaatcctta tttatattgc
     1801 tctggattat tattcaagag aaactttatt ccctagaaat tctcgcagcc acttggcgct
     1861 atctttccag attctctgca agttgttgtc ataatcaaca tggaaaagac caaacttttt
     1921 agtgtagcca cactcccact caaaatcatc cattaaagtc cagataaaat aacctctcaa
     1981 atcaacacca ctctgtattg cttgataagc acttttaaag tgatttttga gaaactcaat
     2041 cctctgccag tcatgtactt caccattttt tagctcatct ttcagagccg ctccattctc
     2101 ggtaatgtac aagggtatag ccccatactc tttgtgtact ttagaaacta tctccctgag
     2161 tccttcagga tagatttccc atcccatatc cgttcgctct gcttccaccg ttactgattt
     2221 agctttaagt atctcgcctc catcatattg aacaacatcc cttgaataat aattaattcc
     2281 caaaaagtcc attttttcag aagctccgga gatatcttgg taacctctgg taaaatcacc
     2341 aaagttgttt acaaaaacct gaaaaagctc ttccgggtat gtacccttaa ataaaggatc
     2401 taaaaaccag cggtttttat agcaatccat aaaattcgct gcttcacggt cttcgggaga
     2461 atcagtaaaa gagtgaacgg gagaaaggtt gagaactata ccaatttttc cggaaaagcc
     2521 tctctcttta aagactttta cggtttcacc atgagcagtt aacaaattat gagccacttt
     2581 gagagctgtg gggtaatcct caatacccgg agcgtgctta cccatttgat gagataaaaa
     2641 agatatacac cagggttcat taaaagtaat ccataaatca acgaggccat taaattcttc
     2701 aaacagagtt actgcataat ctacaaaagc ttttgaggtt tcaggattaa cccagccacc
     2761 atgaaattgc agatgctggg ggagatccca gtgataaagt gttagcatag gcttgatatt
     2821 atttttcaaa agttcctcta taagggattt gtaaaaatca agcccctttt tgtttacgtt
     2881 atcccttccg caaggaaata tccgtggcca ggaaaccgaa aaacgatagg aattaatatt
     2941 cagctctttc attaattcta tatcttcctt gcagcggtga taatggtcgc aggctacatc
     3001 accggtactt ccatcagcaa tcgtaccagg agtatgggca aaacggtccc aaatgctttc
     3061 tcctttacca tcttcattat aagctccctc tatctgataa gaagcagtag caactcccca
     3121 gagaaaatct ggcggaaagt cttccttttt catttgtttt tacctctttc tatatatttt
     3181 tcaggaatct gttaagtctc tctacaaaag ttaccagggt aattgtttac cttgataatc
     3241 ataaaaaaca ggaccttcaa ggtcctgttt ttgagaaata aatagttgat agatttttaa
     3301 agccgattct tcaggctcaa catctgcaga aagatttttt ttgccaagca tataagtctt
     3361 catatagcca ggatggatag ccagcacctt aacaccatat tctttcaggt gattctgcaa
     3421 aatagcagat tgcatgttta gagccgcttt tgacatagca tatccaaatt cttttttacg
     3481 ccagcaattt cctatacttg ctgcctcaga agaaatgtta attactaatt ttctttcacc
     3541 ttttaatatc cagtcaatgg tagagtgagt aactcttaaa ggtcccaagg aattgatatt
     3601 aaaagttttt aacatatcct cgtagcaaag ctcttcaaaa atatttccgg acctactttc
     3661 taaatataca gcagcattgt taataactat atcaagataa gaagtatccg acttaattgt
     3721 cttagctgcc tcttcaaccg attcttcttt agaagtgtca agaggaacga taaccagatg
     3781 ttctggatac tgcgttttca gctttcccag ttcaggccaa tcaggtagat actgaccagc
     3841 ataaacttta aactggtttt caagcagaac cctggtcaaa gccaggccca gccctctatc
     3901 tgctcctgtt acataagctg tgtatacatg ttgtgccact atattgtctc ctctttataa
     3961 gggagcaatt ttaactctcc ctgaaccagc tctattagta taccatcagg gtctttgaag
     4021 aaagcaattc tcactcctcc tgtggcatca aaaggctcga gagtgaaatt aacatcagcc
     4081 tttttcaatt tctttactac ctcgtcaatg ttatccactc tgaaaccaat gtgtttaatc
     4141 cctatatccg ggtcttttac ctccgggaga ggttgcccgc catgtttgaa atagaacaat
     4201 tctagggtag ctccctttgc atccagatac actatcttaa aatcaccacc attttgctct
     4261 aacataccca tcattttaaa acccagaata cgttggtaaa aatctaccga ccgcttaaga
     4321 tttgaaacag taatggtaac atggtcaaag gcttttatca tgtttttaca tcctcctcat
     4381 ataggattat ttatttccac cggtaagttt ttctcccagg acttatagga tgcttcaata
     4441 agctgtagat gagcaagggc cttaactcta tcaactttca cattacccct ctcaacttgc
     4501 tctaagaagt ccctcaaact taatgttatt gaatcttcat aagagatacg atatttttga
     4561 atctcctctt cgccggcaca atttctgaaa caaaaagaaa gatcatggtc ccagatcgag
     4621 atgcttcctt tatccccgta aatgcacata ccctcatccc acggaggcac acaagttccc
     4681 cagctacaga ttaaagaccc tataacccca ctttgaaaaa gcatactgac cagtgctgta
     4741 tcctcaccct ccattttatc tatcacataa gttcctgctc ttgtatatat tgactttaca
     4801 gatccaaaga gccatcttaa aagatcgaaa cggtgtgccc ctgaatcaat gaggcaacca
     4861 cctccagctt cctccttttt aagtctaaac ccttgtaaat tccgggcatt agctctagca
     4921 aaacactcca ccagaaaaac tctacctgtt gaaccagcat cgagcatgga aaaagcctgc
     4981 tgatattgtt tataataacg catattaaag ccaacagtca aagcaagatg acgttcttca
     5041 gccatccgaa caagttcctc agcatctgaa aacttaacgg taagaggctt ttcgcaataa
     5101 acatgtttat tactccgaag tgctttttca accattggtt tatgtaaata gtgaggtaca
     5161 gcaagagtta tcacttcaat gctgtcatca gatagaattt cttcataaga tgtactccat
     5221 ttttcaatag aatatttatg agaaatttct ctcagtttat tctcatcttt atcacaaatt
     5281 actattgatt gaacattttc aatttttaag agagaacgta catgaagctc tccaaatcca
     5341 agtcctatta cagccactcg cataatttta tcctttcttt aacccttaat tgctcccgca
     5401 gttaatcctt tgataaactg tttttgaaga attataaaag tcaccaaaat tggaattacc
     5461 gctataacca tgcccgcaca cagagacccc caatcggtgg aatatcgccc taccagggag
     5521 cgaatggtgg gctgcaaagt cctcttttcc gggctcagaa taaaagttgc tgcatacata
     5581 aattcattcc atattgagac aaactgaaaa atagctactg ttgctattcc gggtttagcg
     5641 agaggtaaaa ctaccttcca gaaagctgta aactctgaag ctccatcgat gatagctgat
     5701 tcccttagag catctggtat agtcacgaaa aaagctctca ttagaaaaat ggaaaaggct
     5761 actcctaaac cagtataact ggcaattaac cccgttaaag aatctaaaag actcatttcc
     5821 ttaagcatca ggaacaaagg gataagagtt acctgaggag gtatagttag agaagcaata
     5881 agagcaagaa aaatagcatt tctccctgga aactttattt ttgcaaaagc gtatcctgcc
     5941 atcacagaga caacaagtat caaaaaaatt gaaacagcag tgactattgt tgtattcatg
     6001 aaagattttc ctatatcaag gttttctata acaaatttat aattatccag catgaatttc
     6061 tcaggccaga gggggcctat aaaaatttcc gagcggtttt taaaagaatt tataaccatc
     6121 cagaccagag gaaatattgt aactacaaat acaataaata gaaaaatata tctaactata
     6181 tcaccagctt ttatttttaa tttaatattc aagagtatca gcctccctta tccggagttg
     6241 aatcacactg gccaaaactg agaaaatcaa tatcattatc ccgatggctg cagcataacc
     6301 catgcgagga gtaccagttc ctacacttcc aaaggccaga gtatagagat aagtagccat
     6361 aacttcagtg gcatgatttg gtccaccagt agtcatagcc caaatgaggt caaaaacctt
     6421 aaagccacca ataacattaa tgattactac aactgtagtt accggacgaa gcaaggggac
     6481 agtgataaaa aggaaccttc ttaagctatc tgctccatcc acgctcgctg cttcataaac
     6541 ttcatctggt attctttgaa gcccggcata aaatataatt agataaaaac ccatggattg
     6601 ccacagagaa acaaaaacaa tacaagggaa agcccactta gggtccgcaa gccagagctg
     6661 tttccatgtc tctaaaccca ggttctgcaa gatggtatta agtaagccaa aattcggttc
     6721 aaaaatcatc gcccacagtg aaccagttat aatgaaagaa attatgttgg gtacaaagaa
     6781 gctcaccctg aaaaaagtat tcagaaagtt atttctaacc agcattactg ccaagaccat
     6841 ggggattaag gtttgtaaaa tagttgtagc aataacaaaa ataatgttat ttttcagagc
     6901 aagccagaaa accgggtcat gtaaaagatt gatgtagttt tgcagaccga caaagactct
     6961 ctctccataa ccatgccaga agctcaggct caatccaata gcgtccagcg agggataaat
     7021 cacaaaaatc acaaaaaaga gaagtgctgg aataatcatc agataaccaa aaatatgctg
     7081 actcttttct aaagaacgcc tttttctcaa attacccgcc tcctttttct cctggagagg
     7141 aaaaaagaaa tcatcctctc caggagaaaa aaagtgctat cttcctatta ccctttgtac
     7201 gtcgctttct atttgagcag ctgcttcttc cggagtaagc tgcccaccca ttaacccttg
     7261 aaccgcatta gccaccgcat cccagctacc ttccccatac aagatatgag gcgctccatc
     7321 ttccagccag gaagccatct tctttatggc cgggtcttcc agaccttgta aagcttccat
     7381 tgtagctggc atacctcgtg cctctaccat agcaggagag taaacttcag ggctgtacat
     7441 aaatttcaaa aattctaaag cttcttcaat atgtttactt cttgctgata ctgctaagtt
     7501 ggctccactt tcattaccgg tccagtgcct tttcccgggt gcataagccg gtgtttcaaa
     7561 gacatcccag ttgaaaccct gaggcgcttc tctatcaata gtaacaggta accaggaacc
     7621 actgtagaac atagctcctt tacccatata aaacatggtg agcgcctgtt cgttagcaat
     7681 accattaatt ccaggagcga agacttctcc ttccaccagt tttttaacca gcttcaatgc
     7741 ttcaataact ggttcgctat tccagcttac ttcacgctct ggatcagtgc gacggtctaa
     7801 ttcaataagt aaagtggggt catctgtaac ctgagtaatc aaaggcaaaa agaaatcgca
     7861 tcccagccac ttatctcgat tgccaaaggg aagtggctga atagaactgt ccattccacg
     7921 aatggtatgg gaaacttcaa tcaaatcatc ccaggtctcg ggtatgttca agcccatctt
     7981 ttcgaagatg tctttgttat agaaaatacc aaaggtttgt gcagtccaag gaagggaata
     8041 taaatgccca tcccaggtaa actgtctaat agtaccaggt gcaaagcgac tcagaaattc
     8101 ttcatcgagc cattcattaa agtcaatggt gactccccct tctccgaggc ttctgaccat
     8161 aacatgggga gcaagaatat caggttcact ttctccagca actgcagctt gtagtttttg
     8221 aaggtactcg ttaaggctaa aaacttgctg ttcaaccctg atttcaggat gctcagaatt
     8281 ccacttgtcc actattttgg gaaaagctgt ttgaaagtca tcaaaccagg tccacattgt
     8341 aatggtaaca gcttcttgag cacctgcagc cgccaccaat aatagcccac ttaaaatcaa
     8401 agtcgctatt gtaactttta ctttacgcgc catgactata tcctccctat cagattttac
     8461 tattattaac tccttttaga actaactgcc acctcactct taagcccgca aaaccccatt
     8521 agaaatacag ttaattattc aggaattatc tcacctcctt taaaacaatt attgtttcat
     8581 cactctagcg aagaagtcag tcagaaaatg tggcataacc agctacgaaa ggaaaattac
     8641 tgtatcttag ctccaaagat aatgtttata tctcctttat tttctaaccg ccttcctaac
     8701 aaaaactact tctcaattcc cttagcctca tcaccccctt tcgactctat agtaaatctt
     8761 atactttgca atagaagctg ctggtaaatt ttattttcga aacttttgca ggaatggcca
     8821 ggcataagat aaattatttt tgcggggcca aaataagtta tccatcctgc agggaagatt
     8881 tccttattac tgtttttata gtgcaaaaag agctctcttt ccgaaaagtt gtcaaattca
     8941 aacaggtaag gttcgtcttc aatttcaaaa acattaattc cctcgctgat agggtgtttt
     9001 ttactttcag caaaaaattc taattttgca agaggtgggt gctttatgaa tcgtgctcct
     9061 attatgtgct taaactcatg tcttgcctgg agcgatatac cacagtggat agccactaaa
     9121 cctttaccca ccgagaaata gcgcagcagg tttccggtct gtaaatcatt gagcggttct
     9181 tcccatctat caagatagga tacgaccagc tcgaactgct caagacgaga atagtcgctt
     9241 aaaatttctt catagtcttc agaaaagcac acttcgaatt cgggctgcaa aatactttca
     9301 attacaggct caatctctct caatggatga taaggagctt tttcattgtc tcctatcatc
     9361 aagagttttt gcttcatcca tcataccccc ctttcccccc aatttgggga ttatagcaat
     9421 tcagtctttc tggcccactg acttattgtt ttactgctcc ggtaaggaga ccagccagaa
     9481 aataccgttg caaacttata agcaggacta aaattggcaa tgtagccagt accgaaccag
     9541 ccataagact tgcataatcc acggcatata accctacaaa attagccagc gctatgggca
     9601 cagtaaaatg ttccgagctt ctcaatacca ccaaaggcca cagaaaatcg ttccaggcgt
     9661 aagtggccaa aagtgttgca catactacca aggaaggttt actaattggt agagcaatat
     9721 tccagaaaat accaaattca gtacagccat cgattcttgc agcatctaaa agcgcatcag
     9781 gtattgctaa cattccctgt ctcataaaga aaatggtaag tccattagca gcaaaaggga
     9841 aaattactgc ctggtaggta tccatccagc cagtctttgc tataaaagca taaagaggaa
     9901 ttacaaaggc atacatagga atcatcaaag ttaataccac agttgagaaa attatttccc
     9961 gacctcggaa cctatatttt gccagggcaa aaccagttag agaacttata aaaaggccaa
    10021 ttatcgtata ggcaatagta atcaaaatac tatttaagta ataacgcaag aaaggcacgg
    10081 aggcaaagag tttgtaataa ctttccagag tccacttccg aggtagaaga gtcggcggga
    10141 acataaacat ctcttgccct gttttaaatg aagaagttac catccagaaa aaaggagaag
    10201 ctacaataat tgcacctaaa actaacaaga cgacaataat caaatgtaga gctgtttcct
    10261 taatataagg gttaaaaaac ttccgggcca tgagcagttt tattcctcct taagaagctt
    10321 ccgttgaaca aaagataaaa taaaaataat acctcctaaa accagaccca tagatgcagc
    10381 ataaccgagg tctaaaaaag aaaaacctct ctgatagagg tatagagcag gagtcaaagt
    10441 agcatccccg ggacctcctc cagtaagaag aaaaggctcg gtgaagagct gataagaact
    10501 tataaccgta atgacaatca caaaaatggt aataggcctc aataggggta cggttatata
    10561 cataaaggtc tgaaagcgat ttgctccatc aatggctgca ctttcaaaaa tttctgaagg
    10621 cagagaccgt aatccagcca gaaaatagag agcatcaact ccaatataac gccatgcgga
    10681 aagcagaata atagacaaaa gagccagatt tggctgttct aaccaggcaa gaggggagat
    10741 tcctatccag ctcagtacta aattagcaaa tccaaagcta cggtcgtaaa tcatgcgaaa
    10801 tacaattgca actacgacca gacttactac agaagggctg tagtataaga cctgaaaaaa
    10861 agttcccgag cgttttccta aagcgttgat tgctatagcc aataagagag aaatgggaat
    10921 caatattaaa agccaggcaa tagcaaaata agtactgtta agcagcgccc taaaaaaccg
    10981 agcgtcttta gttagattaa tatagttttc tacacctaca aaatgagcac tacccaatcc
    11041 ttcaaactta taaaagctca tatgaaaagc ttcaataaca gggtttaaga aaaataacgc
    11101 gaaaaagatt aaaaaagggg caacaaaaat ataaggagcc cacttacttt gaaagcgata
    11161 ataatgctga cttattttat gcttaattcc tgcggggata tccccgcagg aattaacttt
    11221 agcttgcata aataccatcc tcatttacca agctggcgaa gctcattagc agcttcttcc
    11281 agaagatagg cagggtcatc acctaaacaa gctcccaaaa ggacttttcg gttaagtaga
    11341 tcagttgctt caggccaggt cggtgccaaa taaaaagaag gaacttgagg agcttgatca
    11401 agaagaagtc tacctatagt ttgaccacca aagtaagaat ctggagcatc aaatacctca
    11461 gcatcaacta agtccttacg tggaggaatc atgccggtct taagataaaa ggaaaccaaa
    11521 ttatctttat tctcaagcat tgcgtaactt aaaaaagcta aagccgcttt gtcagtttca
    11581 gagttagaag gaacaataga aagcattgtt cctccccagg ttgaagaagt gtaaccccct
    11641 ggttgccatg ctggtaaaga ttttgcccgc cacaagcctt cttgctccgg aacattagct
    11701 ttaagaaaac caacatacca gtcaggacca aaatctgaaa ctacctgcga tgctctataa
    11761 ggtgcccacc aaggaggggt acctacatcc tgaggtgaag tattagcaat ctcgtgttta
    11821 actacagcat cagaataata actaagtatt ttctttgcta tgtcattatc caagataaca
    11881 ttagcttctt catcaaacac ccctccaccg gcttgaatca accacatcca gaaatgaccc
    11941 cattcagcag tactcgcagc tccattacca agttgtagca cgtatttccc ttgttctttg
    12001 aacttcaaac ctacatctac aaattcatcc caggtctgaa tattttctac gtcaattcca
    12061 ttttcctggt aaatgtcgct acggtagaaa aataccgccg gatcaagatc gaaaggtatt
    12121 ccatacagcg ccccatgaaa tgtgaaagga gctaaccgag actcatagaa tttatccaaa
    12181 tagccacttt cttccaggta ggaagtcaga tccagaagat ttactttatc tcctctgtag
    12241 aacttagcca cctggcttat ttcaatagta gctaaatttg gagcacctac acctgtctgg
    12301 actgcgatga gaagattgtc atgcatctgc tgataaggat agtcttctac tttaactttt
    12361 atatctgtat tagctgcctc aaaagatttt gctacttctt ctaaccacgc agtctgagag
    12421 ccaaaagacc agacttttat ttctgtttgt gcccccgcta cctgaacgca gaacaaaacc
    12481 gtaaacaaca gtactattcc ccacttccat cctttctttt tcattgcctt ttcaacctcc
    12541 cgcttagaaa attaatttaa cgaaagtgaa taaattgaaa caaaatagaa aaaatagact
    12601 ataaataaaa attatcacct ccttctaaat taatttatta cttctttttc agttaactct
    12661 ggagaaacaa aaatttttaa taatctccga ttttctttac cctctaacaa agcctcttct
    12721 aatgcttcta aaggcacttt taaagaaaac agaggttgag gattaattct cccgaatctc
    12781 agaagagcta tagcatcaga ccaatcatta ccaactccag cacaggaacc aaccaagctt
    12841 ctttccccca gaacaaaatc atagggtctg agtttcattt catcttcctg gttggttatg
    12901 ccaaaagcag ctattcttcc tcctggtcga actattttta aagcagtttc ataagtggaa
    12961 actgtaccaa ctgcttctat cacaaaatca gcccctcttc cttcggtcaa ttccttaacc
    13021 attcgaacca catcctcttt tgatatatct attaagtagt ctactcccat cttccgcgcc
    13081 gcctctaatc tttctttgtg ttttccaatt aaaatcactg gcgcagcagc tctcagtttt
    13141 gctatttgag tatgcaaaat tcccaatgtt ccgtctccga tcacggcaac actactccca
    13201 aaggaaactt ccatagcctt agcagcatga atggcacacg aaatcggctc aataaaagct
    13261 cctgcctcaa atccaacctc atcgggtagg atgtgaattt gtttcgctgg tacttttaaa
    13321 tattcagcaa aagctccatc catatgaatt cctaattgcc ggaattcggg acaaataagg
    13381 ctttgacctc tggtacagta aaaacaatga ccacaactta gattaatatc agctgtcact
    13441 ctttgtccta ttttgagatt tttaaccttc tctcccacct gaacaatttc tcctgaaaat
    13501 tcatggccag gtataagagg cagaaattct tttgagtatt taccagtata aatgctgtaa
    13561 tcagtaccac aaatcccgca aaactttacc ttaaccagaa cttcttcagg ggaaatttga
    13621 ggaactggaa catcccttat tttgatttcc ccgggctttt caactatagc agctctcatt
    13681 ttttcaggaa ccatactttt aacctcctcc cctatcctct gggaacaata gaaattgcat
    13741 cttctggaca aattacggta cacatagcac aacttgagca gaaactttca ttaacaaatg
    13801 gcttcccatc tttcatagaa atagcaggac aataagaagc agcacatttc ccacatctgg
    13861 tacatttgtc ctcatcaatt tctgctcgat gataaaggaa ctcaacctca gtagaagact
    13921 ttagatattg aagacctaag cctttaaagt cgtcaatttt ctcatatcca catttttcca
    13981 tgtatctttg tagccagcga ttagctctgc caatgaaatc aattccattc aagacaattc
    14041 cagtaagagc ctggcaagct ctggctccta acataatagc ctcgacaatg tgctcaggag
    14101 aggtaatacc tcctccagca aaggtctcaa tgccaggtat atttttcgaa attagagctg
    14161 ttgctttata catactaaat cggagagcag gcccaccaat atcagagaga acattggcat
    14221 tatcgcttac tcccggatat ttccccttac ctccattcca aatatctggt ggagggactg
    14281 caaagtattt atgggtagtg acaacgaact tagcaccagc tctggctgct tcttcagtaa
    14341 ggtataacat cctaggatat ccgacttcag gagttaattt aaaccccacc ggtagctcgg
    14401 tagcctttac acaggcctct aaaaccggca aaaatacttc tggagcagct cccatcatca
    14461 tcccccattt agcttcagga ggtagatttt gggcatctaa atgccccatt gcaccaactg
    14521 gacaaccggt gttaagctca ataatgtctg gctgtagctc gtctacaact cgcttggtct
    14581 ggtctgccca ctttgcggga ttcgtatcat gaaccagaac ctgggcaatt attggaacat
    14641 ctggtggtag tgaaggtcga agctcgctaa ccagctcttt cccccagctt aagtaattaa
    14701 aaagattttt agcagtataa atgtgagcga tagcttcttt atgttgagaa tcaagagtaa
    14761 ccttcatcca tctaccaaca taattttgcg ttttaaaaag ttcgggaaag tcaagctctt
    14821 tttctgcccg agtgggttcg ccaggtacta aagctggtat ataaataaaa cctgcgccag
    14881 cttcgatgta cttcataaaa agttcagctg cctttttccc aggaaaccag ggtttatcca
    14941 gattatgagg agaaacacca aaagggctcc tcatttttac tcctgccaag ctggtagaaa
    15001 tatctatctt ttttgccatc tctcaatgtt cctccttttt ctcactgaaa cagaatatct
    15061 tttcttcctt catagggcgc tttgataagc aatggtttca gaaaatcagc ggttttagta
    15121 gcgccatctt cccggctcat agttacatcc tcatgctcat aactgagaac ataatcataa
    15181 ccaatcattg ccaactcagt tattactctt ttccagggca ccgaccccca gccagggatg
    15241 cggaaacgaa aacctctatc tattctggtc cacggaccgg tgggaattaa accgtctctt
    15301 ttcacattat gttctacaat ctctccatct ttggcatgca cgtgataaat tctttgtccc
    15361 agtatttgaa taaaattaac tacgtcaata cctaaatata caaggtttgc cggatcgaaa
    15421 ttaaaacccc aggctttctt ttctcctaat aattccaagc ttctctgagc agtatatatg
    15481 tcatagacta attcattagg atgaggctcg tgagcgaatt tcactccata cttttcaaat
    15541 tcgtccagta tctttcccca tctttctaca aacttttgct ccatttcagc ccatccctta
    15601 gagtaaggcc aaggaaagaa acgcccgaaa ttctcacagc caataaaacc acagacaacc
    15661 ggaacttcaa gcgcattggc agcctgagct gttttaatca ttctttccat tccatatttt
    15721 attttttctt ccgggcttcc cttaaaaatt gcatcagtgt cttctccgta cggtcccaaa
    15781 actaactggc cttccggatg attgctcaaa gcagaaatga atagtcctct atcttctacc
    15841 atttccctaa tatcttttgc ataggaagga ttatcaataa ctttttcgat atccagatgt
    15901 gggctcccac taaaagcagg tatctccact gcttcataac ccaatttgca tattttatca
    15961 aggacttcct ccagtggccg gtccgaaaaa ttagccgtaa aaaacccgag cttcataaat
    16021 tttgccccct tttttcgcta aaatctaaat ttcccattta atatgcttcc actctaaata
    16081 ctcttctaac ccttggtggc cgagttctac ccccagcccg ctttccttcc agcccgggta
    16141 aggtgcgtac aggctgtcag ggctaatatt gtttatagct accgaacccc attccaggcg
    16201 ctctgctact cggaaagctt ttttcatatc ttgggtgtaa agataggcta caagaccata
    16261 ccggacgcta ttagcttttt cgatagcttc ttctatactt tgaaaagaat cgatagccac
    16321 aacaggacca aagggctctt cgttcataat tttcatatca ggcgttacct cagtaatgac
    16381 tgtgggttca aagaaaaatc cttcctggag tcggggatca tctggttttt taccccccca
    16441 aagaactttc cctcctttaa cttgagcatc ctctataaaa tctttaattc tctctattcc
    16501 cgcttgatta actaaaggac ccaggtcggg agctggctct tcaaaagctc cacccaagcg
    16561 caatttttga gtagcttcta caaaggcttt tatataacta tcttttattt tcccctccac
    16621 caatatccga tttaccgagt tacaaatctg tccggcgttg cgaaaggctc ttttcagtcc
    16681 atctttcact gctttttcaa agttagcatc agcaaagaca atgaacggag aatgcccgcc
    16741 taactctaga attaatttct ttaccgttgg agctgaagct ttaataaatt cttttcctgt
    16801 ttcagtagaa ccagtaagag ctaccagccg tattcgaggg tcagaaaaaa tcttctggct
    16861 gatgagagta ttgtctccga ttattacatt gagtgctcct tccggtaagc tcgcttcatt
    16921 aattgctttt acaaattcaa tagtggaaag tggggtttcc cgagaaggtt tgactatcac
    16981 tgtacagcca gctgctaaag ctggagctac tttccaggaa atcaagctca ctggataatt
    17041 ccaggctgct accgcaaata ctactcctac tggctggcgg atagccaggc tttggagatg
    17101 agaagcacta ttagggggaa tttctcctac aatattcatt gccaaactac cataatattc
    17161 taagcaatca gctgctccca gaacttcttt ttcagcatcc gggaaagcct taccttgttc
    17221 ctgagccagg agacgaccaa tatctttagc tatttctcga actttatctg ctgcccggaa
    17281 taaaatatgc gctcgctctg aagccaattt ttgagaccag ctctggaaag cttgataagc
    17341 gccctcgata gcttcctcta tttgattagc agtcactttt gagacagtag caaaaacctt
    17401 gccgtttgat gggtcagtta cttttatctc tccttctcct tcttgttcaa tcccatttat
    17461 aaacgatgag taatgaagca ccatcaattc tcctttccat attgagaaaa aatatcttgt
    17521 agagagtcat gaaaattttt gaactgctga tattcctttc gataagctct caggcgatcg
    17581 ggttcaggca aaaacttttt tcttaccttt atccattctt tcgccaattg acttaccggt
    17641 cgtttaaggg ctgaagaaaa ggaaagcatg gctgctccca taatgcctcc ttctgtttcc
    17701 tcaagcgccc agatctcttt ttcatatata tcagctttaa tctggcacca caaattactt
    17761 ttagctcctc ctccaatagc tgtgaggtgg gaaatttttt cttgccggag cttttcaatt
    17821 tcttccagac tcatcttaag atcaaaagaa actccttcca ttactgcccg ggcaaaatgt
    17881 tttctttgat gatggggtaa aattcccaga aacacccctc gagcatgagg agagcccata
    17941 agtcgctctc ctaaaagata gggaaagaat aaaagcccag cagagtctat tgtttgagct
    18001 tctttgtcaa tttcagaata agaaactgtt tttccttcat cttgataaaa agcatccctg
    18061 aaccacctca ggcttcctcc accactatct aaaatgccaa agctaatcca gccatcaaca
    18121 ccatgatgaa ggttcataag tttaggagaa attaaagggt aagaagagaa aattgataca
    18181 tcagcagcag ttcctgctac gtcacaagat ttaccttctt ccaccatccc tccccccaga
    18241 agcaggcaaa gcatatctcc tgctccgcag ataactggaa ttccggaagg aatatccaga
    18301 aaatgagcca cttcttcttg tacctgaccc acaacctgga cggagggaat tataggagga
    18361 agtttctctt gatccaggtc aaaaagagat agaagttcat cgctccagtc cttttctttc
    18421 caggaaaaaa gaaaactgcc ggaggcctca gaaaaatcag taaaataact gccagttaat
    18481 ttgaagttta aataatcttt agcagttaga aaaactgagg tttggttata gacttcagga
    18541 atatactctt taatccattt catcttgggg ccggtccaat taggaatgat aaggttagca
    18601 gctttattta attgctcttg aagaggtatc tcctgggata cctcttcaca tattttttga
    18661 gaccttttat cacaccagat gggcacttgt tttaataaaa cccttccctt attgtccaca
    18721 ggaaccactg catgcatttg agcagttact cccacagcta ctatctccat ccgggtatct
    18781 tttaaattta aactcctcac gcactggctt gataactccc accattcttc gggactttgt
    18841 tctgcccaac caggatgagg agagaaaaaa gaaggcgacc ttccctcaat ttgcctcact
    18901 tttcccttct catccactaa ggcagcccgg acgctctctg ttccgatgtc tattactaaa
    18961 acccccctat ctttgctcac catagactac ttttcctttt ctacagttat ccagatgtag
    19021 gaaagaggaa gaggagagga atttatagtt ctatggaact taccaaatgg aatgtacact
    19081 acatcatcct tctgtatctc aaactcttct tcatcaataa tcataatgcc ctcgccctca
    19141 gttataaaat aaacttcttc tttgggagaa tgactatgac ctttggtgtt acaacccggg
    19201 taaatgactg taaaacctac atcaattctc cccgattcag tgttatcttt agtgaggagc
    19261 ataagagtat ctcttaatac cttgctatcc gagcctttga gtaattcagc aaatgcactt
    19321 ggaccttctt cggggacggc atccagcaaa ttttttttat aatatctcaa ttaaaaacct
    19381 cccttcctca gtggggacga ccttccaaat aaactttata ttcatattta tccgctctta
    19441 tcactgaaat tactacttca aaaggggttt catcttccag ataagtagtt cttaccgtgt
    19501 gaaaaagggg gattctttgt ggtatctgta aaaggtctga ttctaagggt ccactcaggt
    19561 aagcctctat agtttccacc gctcggtgta gttgcaatcc atattctctt tctaataact
    19621 gatacagacc ctcctggcgc aaaatttctt ttttaaattc cagacccgga attacccgag
    19681 ccaaaagata gttactcata aaagctatgg gtatattgtt agctatcctg gttcgctgca
    19741 ccagatatac tttttcgtca ctttccaggt tcagaagaga ggcaatctta ggaggagcag
    19801 taatcaactc cacagttaaa ctaccggtcg taacctcaat acctttttcc ttaagagtct
    19861 cagtgaagga actgatatag ttcaaattct gggtagtctt gggttcttgg accatagtcc
    19921 cctttcctct tctacgagaa acaagccctt ctttttctaa ctctcccagt gcgtttctaa
    19981 tggtggtacg gctagctcca aatcgttctc ctaactctat ctcagaagga agcaaatctc
    20041 cgaccttata agttccgttt tctatttctt cttttaaaga tttgtaaatc ttaaaataaa
    20101 aggaagtttc ttctttatca acttgtttat ccatctttgt tcccctatcc tttttattaa
    20161 tcattacttt taagtttaaa agtaattact tattttgtca atagacaaac ggttgaaatt
    20221 cgcacaaaac tggtactaat taaagctttt attttaaaaa gacagttaat gttgactaat
    20281 tttatcttga acttgtagta tcttttaact tgaaaaaaca aaattatgtt aatacttaaa
    20341 tctgggtttt actggtaaaa taatagagac taactttgag gaggtagata aaatgcaact
    20401 taaaagtcgc caggaagtca ctcagttttt agaagatagt aaaatcattg ctatcatcag
    20461 agctcaagaa gcacaatcac ttaccgaagc tgtaactgca ctccgagaag gagggataaa
    20521 ctgcattgaa gtgacaatga ctaccccggg agcactaaaa ctcttagaag aagtaagtgg
    20581 caaagtagaa ggagtgctct ttggggctgg aacagtgctg gacccagaaa cggcaagatt
    20641 gtgtatttta agtggtgctc aatttttagt tactccttct ctcaactttt ccgtgatgga
    20701 aatggcccat cgctacgata tccccataat cccaggagct ttaactccta ctgaaattct
    20761 tagcgcctgg gaaaaaggtg ccgatgtggt taaagtgttt ccagctggaa gtttaggccc
    20821 aagttatatt aaggaattaa aagggccttt ccctcaaatt aaactctgtc ccaccggagg
    20881 agttaacttg gataatcttt ccactttctt aaaagcaggg gccagttgtg cgggggtggg
    20941 aggagcattg gtgaaaaatg aattcattcg agaaaaaaag tgggaagaat tgaaaaaact
    21001 ggccaagcaa tttaaagaag caactcaaaa tggctaatca gcccacagac caacttttgc
    21061 ttgacgagct tctctttcca gttgcaagaa gcgttcagca tatttcacat taggaggaaa
    21121 ggtggccact cgggcataac cgttggccac caaccattca ttaaccatta gatcatccac
    21181 ataaacgtag gctaaaattc gcccataacg gtctcgctct tccacatcca attccagccg
    21241 tactcttttt ccttctacca gccgacgatt agcctcataa gcttcccgag catagtattc
    21301 tacctctttt tgaggatggt gaagttcggg agtgtctact ccaatatagc gtactttttc
    21361 tccgttagcc aactctatag tatctccatc tataactctt gttacaaggt aagtaccttc
    21421 tttttcactt tggaaataaa aaaagagaaa aaggataatt actaaaaagt atatccactt
    21481 atttggagaa gagctaaaat tgcgccgctt tctcctcata ctactcgata taagggctct
    21541 tctccctgta aaactcgaat gacgttcaaa gccgcaatcc ttcccatttt tatattggct
    21601 tctttagtat gagcagaaac atgaggagtg acaataacat tgggaagaga taaaagagga
    21661 ctctcaaaag gaggctcaca actcaaaaca tctaaagccg ctcccttaat ccttttctct
    21721 tgcaaaaatc ggtaaagggc ttcttcatct atcaattctc cccgagcagt attaatgaga
    21781 aaagcttcct ctttcatcaa ggataacaac ttctctccca ccattttttc cgtatctttg
    21841 gttaagggag catgaaggct aataaagtct gcccttttaa ataaatcttc aagagaaacg
    21901 gaagcaaatt ctttatcttc cactaagggg tcataataaa tcactttcat tccaaacccc
    21961 tggcctcttt ttcctacttc tttgccgata cgcccccaac ccactatacc caaaacttta
    22021 tcttctaact ccacccccag aaaaggagaa ctccagactt tatttttcac ctgctctaaa
    22081 agggtgggta atcctcgcgc cgaagccaga attaaaagaa gagccatatc cgctaccgat
    22141 tgggagttag taccaggagc attggctacc actactttgt gtcttttggc tgattctaaa
    22201 tcgatattat ccactcccac gccatgttta gaaaccaccc ggaggttaga acagcgagaa
    22261 aaaaccactt cccctatccg gtccactcct acaatcactg cttctgcttc tccaatgagt
    22321 tctgctactt tttcctcttt ctccggatcg atatttttct taaacaccac ctccaagcca
    22381 gctttattca atagctcccg aggctcatct gacccttgag aaaaagaacg ggctagtacc
    22441 actacttgac tcatttttct tcctccctga agttaacctg caccggacct ctaccctctc
    22501 ccgcttcaaa gtattcccgg cgagaaaatc ccaagcttcg agcaaagata ttagcaggga
    22561 atttttctat catagtatta tatttataaa ctgtatcatt ataaaattga cgagcataag
    22621 caattttatc ttcggtctcc gaaagttcct tttgcaaact ctggaaattc tcattggcct
    22681 gtaaagtagg ataggcttct accacagcaa ataattgccg caaagcccga gttatacctt
    22741 cttccgcttc tcctcgctct tgcggagttt gagctcccat agccatttgc cgagctcgag
    22801 taacattttc taacacttcc ttctcatgag aagcatatcc tttgactgtt tccaccaggt
    22861 tcgggatgag gtcatagcgc cggcgaagct gcacctctat ctgcgaccag ctgttatcca
    22921 cccgattttt gtacaccact aaacgattgt aaagaacaat gagataaatt accaaaacta
    22981 ctactattag taaaattatc cagaccatct aattcctcct ttcaacctgc tcgggagctt
    23041 ccacctccac cgcctcctcc tccaccatga gaaaatcctc ctcccacaga agtggaggta
    23101 gaaactgcct ggcgtaagtg agctaaattt tccatccggt tagaaaaatt atctaattga
    23161 gacaaaccat cgagagaagc taaggtaaaa gtcccaccta ccggatgata ccaggtagga
    23221 actactaaag cctctcctct ctcctgggcg tagagcttta ggtttttcaa aagttcttct
    23281 gctactccca ataccacggc atacacgaga tagcggtccc aaatatgaag taaaagaggt
    23341 ggagcttcct tcatcaaaga aaaatcagaa ataaacctcc ggaaggcttg ccaacgcttg
    23401 aactccagtg ccccctcctt actccaccga ctaagagatt ttagagataa aaaagcgaag
    23461 aacaaagcca aagggataaa aggtataacc aaaaaagctc cccaaaagct atttcctgaa
    23521 gttaaagcca aaaagacata aaatattgct attccactat aaatcattcc cagagtaaaa
    23581 aactgcgttt tcttaatctc agaaagggga tctaaaagag ggaaaaagtg ctcctcccaa
    23641 aagctccggg ctttttctcc ccattgttct ataaaagacg aaaacttact tttatctcct
    23701 ctaaattcgc taccccactc ttcaatatcc agagtagaaa cactctttcc ctctttagaa
    23761 atacgatgaa gaagctccaa aacttcttgt tctaaaggta aaagggcgtc atttttattt
    23821 tcagatattc ctctctcggt aaaagtaaaa ttcaaataat tttttttgcc tataaacttc
    23881 ttttcctctt tttcttctac tttcagatat ccccgacggg ctaaatctaa aatagtagcc
    23941 aaaaaagctc tactcatcac ttgaggagaa gcttcctgtt gacttagtat acttcctaaa
    24001 agagaaggag aaacatctcg aggaggttct tgctcatatt ccctttcata gccaattttg
    24061 ggctccttgc catagcgaaa atagtaataa aagaaaagaa tgatatagag aatacctccc
    24121 ccaactaaaa ggaaagagaa aaagattgtc cagcgggaaa cctgaggccc ttcgttgatg
    24181 agccgctctt cttcttgaag aatttcttca tatctattat catctaaagg agaaaggagg
    24241 gggaagattt gaggctcaag gagtaagcga gcctctacaa aagtatcaga aggaatggaa
    24301 gagagataaa aatcaaccct tttataatcg ggagagaaaa ctatttctcc cggctctact
    24361 tcagcatgaa caaaaagttt taaaagctca ggactcgctt gaggaagggt aaaagaaacc
    24421 cgcaaatctt ccacttccgc atgttcatct tctaaaactt tccagtaaaa ttctgccaca
    24481 tcccgatagc gcttcactac ctggcgcaaa gtgtatttta ttctaaaacc tttaatttca
    24541 tcttctgcct gatagaacca cttaagagat ttactatgtg cttcttcttc tacttgaaaa
    24601 ggaagcggct ctccagtttc tatgctccac actcctgaaa aatcgatatc ttttacccct
    24661 cgcaagagaa tggtaagctg agcccaggaa aaactacccg aaaaagaaaa ataacgctct
    24721 tcttccacct ctacatctcc attttcttgg agaacaaaac tttgattaat agagggatga
    24781 aaataagatt tagaaaaagc gaaagagggt aaaagaaaaa gaacgagaat gaagatccaa
    24841 aaggatagga cgaacttctt catctttcct ccctcatata gtaaactttc gctaatatat
    24901 cacagattaa ggagaaacaa aataaggcta tgttttttct acaaaaattt tggggagcct
    24961 tcctcactct accggggata ttagtcacca tacctttatt tttattccta tggggtttaa
    25021 taaagaaaaa aatcaactgg tcactcttat ttctggcatt gttgagttac tttttctcta
    25081 ccggggcagg gctttatctt ctaccccggg aagctccgtc cagagatgca accgctactc
    25141 ctcaagctat agtagtcttg ggaggaggac gagtctcttc ttttcttcgc acttaccagg
    25201 cttttctcct gtgggaaaaa gagcagatac cccttgtttt aagtgggggg aaaatagaac
    25261 aagggagcaa aaacagcgaa gcggagatta tgaaagaatt acttattagc tggaaagttg
    25321 aggaagaaga tatatatctc gaacctgtat cccgcaccac ctgggaaaat gctctttata
    25381 ccacagaaat attgcgagaa aaaggatggc aaacttttta tttagtaact tcagaaatcc
    25441 acttaaaaag agcttgcttt tcttttcacc atctttatcc tgaagcccat attattccag
    25501 tgagcggcga ccctatttat aatttcagcg ctttttcctg gatggatttc ttgcccagtg
    25561 gagaagcttt ttctgcctgg gtagagataa ttcatgaata tataggttat ctcttttaca
    25621 gagtagttta cggtacagac agcctgcggc gctaaaaaga taaaaaacga agagaaaaaa
    25681 gaaaatatat atattaagga cctggtttcc cgagtatttt ataactatga gtcctaaaaa
    25741 gctgaaaaat aagctgtttt gcggcaatat tttttcctgc aagctccttt aatttaccaa
    25801 atctcagcat caattatgga gagtctgggg attgatacaa aaacatagtt ataattttcc
    25861 gggaaattag ggttataata gactatcaaa atgcttacca tccggaaact accagggtac
    25921 caagaggata ccagaggggt atttaccagg gtaaaagaat ggttggtaag taaaggaaaa
    25981 gattaggaag gggggaaagg aaagtatgac accagaagat agactcatga aagtaactat
    26041 cataattggg attgccctaa ttgtactaac tgcattagca atttatttaa tatacggtgc
    26101 ttaccttttt aaagaagagt ggccatggat gagattctct attataatgg cagtattaac
    26161 ttgtctttgg tcatatgcct tttggtttga tcgtaccacc atggacaaag atatctctga
    26221 taggttatgg tatgtggttg tgtttgtccc gtttgatgtt cttttaatgt accctggtca
    26281 taaagcactt gggctttatg tggatcatca ttttgaaata tttataatta ttattatgat
    26341 catacatggg tttggttttt attttataca aaaggaaata caaaagtatc taaaacagaa
    26401 gtatctaaat aaccgatcta ataactatga aacatgacgc ggtccggtgt atcatcgcta
    26461 tttttccaac gcggcatctt ctaaaaaaga aacaggcggc atgaagccgt ttgttttacg
    26521 tccctatcat tgaccctttt tactttctgc cctattatgt tggcatcgtt aacattgcga
    26581 ccgactggta gtagcgaaaa gcgaaataat atcggtggct gtttttcagt tactgcccga
    26641 gatagtgctt attttattca attcagaagg cgccacctta caaaatgtgc ggtaaaattg
    26701 ccaatatggc aaaaataccg cacaaagaac tacaagatgt cagaaattgc agaatctgat
    26761 attgaagaat tacaagccaa aatatcaaaa gaagctatta ccagaaatta ccaatgtggt
    26821 aaaacgattc atgaagaatt acgcgaagtc gtcctggcgg tggaaaggct ccgagtagcg
    26881 aaatcatttc gtccttcaga agatgttaaa gccatagatt ttaattctaa gggcattaga
    26941 agcgttagtt gtttcttcca tttcattatc tggttagaat gtaccccaga tttactggat
    27001 atctctcgta tggtcttatc tcattcggtg cttctcgtgc tattttagct tttagtgctg
    27061 catcatactt ttttcggatg tcacccattt tcttatcttt ctcctttcca ttttacagat
    27121 taaatacacc ttattactct gtccagtttt cggggtccat tataaatact gagcatcctt
    27181 cagcagccaa tagacttcgg tatttcgaag atgtttgttt tccttggcat agtcaaaggc
    27241 ggttttgccc tctttgcacc taatttttcc atcggctccg gcttctagca gtacttttat
    27301 gacctcaggg ttttggttaa aacaggcagc ctgcattagt ggagtccagc catcttcatc
    27361 ccgggcattg acctcggcac cggcttctag caggaccttg atgacctcag ggttttggtt
    27421 tgccccggca gcatgcatca gtggagtcat accattttca tcccgggcat tgacattcgc
    27481 tccggcttca attgcctttt ttacctcttc gaggcttccg gttttcacaa gctcggtaaa
    27541 gtcttcttgt ctcagagcca atgaactacc aatcaaaatg atacaagcga taagaccgac
    27601 tattaaggtc tttttcatcc ttttctctcc ccttcctgaa tgataacata tactaaagct
    27661 cgcattttcg cactgtgaaa aaagacaccg gagactccac aatgaaggtt gaacaaaacc
    27721 aatacttcat cgaggaggaa tatccgatgg ttcatacaaa ttatacccca tatcttttaa
    27781 aaaatgctcc ttgaattcat gacccacttc gttttctaaa aggtataaac acaccgccca
    27841 cccaaacaag agggcttcct atagcgtgca aattaaaata caatggctat ttttgcccgg
    27901 ggttttttac gtgtatacgt ttcccccggg gtaaccacca tacccccggt aagagctttc
    27961 aaggagttag tccgctttga ctggcggcat attctgcctg ctccgttgta aacccatcgt
    28021 actccagctg tctaattaac ctatcatacg aaaaaggaaa aatacttaaa tatgaccttg
    28081 cagccctgac ggcttgttca taccagtcag ctccacaatt atcagctcca tattttgctt
    28141 catcataagt aaagccatca tgctccagtt ggtctattaa gccattatac gaaaaagcca
    28201 taaagcttaa gtatgacttt gctgctttaa cggcttgctc ataccagtta gcaccacagt
    28261 tatctgctgc atatcttgca tcttccgtag aaaagccatc atataccaac tgtttaacta
    28321 aaccatcata tgaaaatgcc ataaagctta aatatatcct tgcagtctca acagcgtttc
    28381 tttgagatgg tgtttcatta aaaactgtcg gttctggtgt tggtgttgga gaatgcgttg
    28441 gtgttggagt aactattggt tcaggtgttg ctccacatcc tgccaagaat atagccatta
    28501 acagaataac aaggtagaaa tacgctgttt tgccatattg ctttagaaaa accatttttt
    28561 aagacctcct tttttattta attcccctta ttctccaaga gttttagagg aggacttttc
    28621 tcgtgttctc tttttaagtc tccttggttt aagcttaaat agcgtttggt catagttaag
    28681 tcagtgtgtc ccaaaattct ccgcaaagcg aaaaggttca ttccctctct taaaaagtat
    28741 agcgcaaaag tatgccttaa gtcataggga gttactttat aacctaactt tactgtattt
    28801 ggctaacatt tgagaccagg aattagggca aagcggagag aagtttttta acccacaggc
    28861 aagttagatg agaaagagga aaacgaccta aaaagaatta aagaagcttt taggggagta
    28921 acccctactg tgtaattttg gcaaaaatat aaaaataata ccgaaaatgc ttgataatgg
    28981 ttaaactact gctatactgc ttaataggat accgaaaacg tataaaatag gtatttagac
    29041 atagaaaaac tggtaaaact gtaaaagttg ttaggcattg agaaacaagt atttatttaa
    29101 atatttccag gattgtataa ggagggaaga aaatgaaaag gaaaaatttt ttaatagttt
    29161 tacttttggt gttagctgtt tatttatcag gctgcagcgg tgggatagta actccaaatg
    29221 cttatgaagt aggtggagat ttctatgttt gtgaaaattt acttagagga ttttacactg
    29281 ctataagcaa tcgaaattat gctcaagctc tttcgtattg taaacctggt gggtcttcgt
    29341 ttgagtttgt gaatgacata tggaatcttg accagcaata tccgacaact tatataacct
    29401 gtcaagttta taatgtttat gatttttctt atataggaca atcaaggata agtctccatt
    29461 atgatttttc cgatacagca catgacattt atggaggaac atataataca aattatcatt
    29521 acggagccac agcgttattt gaaaaagtaa atggagaatg gaaattgttt taaattggag
    29581 gacgttggat agtctttctt tctgaaaagg tagcgtgtaa attaggaggt attttgaaat
    29641 gtattatggg aaaatggaaa ataaaaagaa acacttcaga attccagaag ttttaacacc
    29701 agaggagcaa gagaagtttt taaaaacctt caataagaaa gcaaaaacag gaatgaggaa
    29761 ttatgcaatg tgtaggttaa tgttagacgc tggcttacgc tgtggagagg tattgaattt
    29821 aaaagttagt gacgtggact tccagactgg caaaatacac gttaagcaag gaaaaggaaa
    29881 taaagatagg atagtgtggc tcaatgagga agctttggac ttgttaagag agtggagaga
    29941 gagaaaacca gagagcgaac tactattcac caccttacaa ggaaaaccta taaaccctcg
    30001 ctatgttaga gatatggtga agaggaaagc caaacaagca ggaattgata aagatgttca
    30061 ccctcacacc ttaagacact ctttcgctac tgacttatac cgagagagta aaaatttaag
    30121 acttgttcaa aaagccttag gacatagttc aatatccacc actgaaattt acactcatat
    30181 agttgatgaa gagttagaga aggaaatgaa attttttcgc tcaaaagagg tagcataagg
    30241 attaggatat tttcctttgc gttaagcggg tgtgacatac aaaataacac atatagtaag
    30301 caaggcgttt tcacaatggt tgaggaaagg gagaatataa agaggtacgg gcagtaatga
    30361 aaaacttaga agaaaaaggt ttttcctgag gagaggaggc taagacaaaa gttaaagaga
    30421 gaggtatgac gcaacaagaa cttgctttta accaaagttt atctccatta taaaagaagg
    30481 agggggattg ttttggtttt tctattaata ataattatta tcattttact ttacattgtt
    30541 ttcgacagag caatggataa ccttaagaaa tttcgtatgg ttttatgccc ttattgtcga
    30601 aacgaactaa agccaacatc agaaccaaag gtaatttact gtgcacagaa gaatatataa
    30661 cgaagaccaa ccatgtccat cattcggttc atattggagt aaatatggta agtaaagagg
    30721 agttaccgtc atacgcaaat tttgtgtatg atccgagaga agcgtcctcc tcttctcgaa
    30781 ttccatcgac gaacttcact ccttgcacca cctccctgga cggggaactc catctaactt
    30841 tctccatcca tcttgagcac tgag
//
LOCUS       DAOSVE010000010        30233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_1029_length_30233_cov_75.026244, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000010 DAOSVE010000000
VERSION     DAOSVE010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 30233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30233
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1029_length_30233_cov_75.026244"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            521..1015
                     /locus_tag="PK016_01690"
     CDS             521..1015
                     /locus_tag="PK016_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HXL01824.1"
                     /translation="MARDMNCFFGIGNLTREPDLRYTPQGTPVCRFSIAMNRSYSGKQ
                     GMVKEVTYVTVAAWSKLAEICARYLHKGSRVAVMGELRSNSWEDRNGSRRVSYEIRAD
                     NIQFLSPPKGVTQEEVEEFTGLGAKLEMEEAEPFVYESGENPLGELEDIPLEKDDGGE
                     IAPF"
     gene            933..2594
                     /locus_tag="PK016_01695"
     CDS             933..2594
                     /locus_tag="PK016_01695"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HXL01825.1"
                     /translation="MKAEKTHWESWKISPWRKTTGEKLPLFRRKVFVLFLFFFLLPLF
                     ALEAQVKIDWGEESFYSQAEVRVRQGKTYVNVEELFREIGGIVYYSPIMNKINLGFKG
                     TAWVLSLDKGVAVHNGREVSLEGEVFVEGNAPFVSLDLLSQLFSFVPSTPTTFATPPP
                     EVTFSPPPGEERYSSFVLLGIRFYSYSEEMRTRVTFDFQGGIPSYSYRMDREENRITV
                     ELSGCDWRQSMSPIIVNDGRINRIELTKTAEGLRVNIWLQNPVQVKEEKKLPGDNPRI
                     YFDLVSLVEAKVEASPEVKPTTTPVASPKIQPTPTSPQVTPGLSYNEVNTKVVVIDPG
                     HGGNDPGCVYNGYKEKDIVLAISQRVKVALESKGYQVFLTRSQDVYPSLNDRYRVANE
                     RRPFVFLSIHCNASPNPQATGVEVFVGGVKPRGEGAGDVASRENQLFLEEGKTEDINR
                     VGNILSSAYYQSSREVSAELGKIMSEKISSTTGQKNRGLKEAPLVVLDGMYYPACLVE
                     VGFLSNAEEAKKLASSSFQEKIAQAIAQAVDDFSRNPKFKSFLGE"
     gene            2596..3228
                     /locus_tag="PK016_01700"
     CDS             2596..3228
                     /locus_tag="PK016_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HXL01826.1"
                     /translation="MKNQTPPSRKKYKEKESSWPGWFYILVGLAIFFAVLYFGELIIR
                     YLQPPEISPSPGVVVSPEEEREEILPAGEEEVVLYFSNPDFTALVGEKRKIKKDSKWM
                     ERIIEELLKGPRDRSLYNPIPPSTRLNAVFLEGDIAYVDLSAEMAKNQSGGTSQELLS
                     VYAIIDTLTAFPEIKKVKILIDGEEIPTLCGHIDISQPLERDEKLIAKVQ"
     gene            3239..4009
                     /gene="rph"
                     /locus_tag="PK016_01705"
     CDS             3239..4009
                     /gene="rph"
                     /locus_tag="PK016_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HXL01827.1"
                     /translation="MGDFYFKKDKATWKGRPDRRRPDELRPVEIHLHYLRYAEGSVLV
                     EAGNNRIVCAASIEDKVPPFLRNSGQGWITAEYAMLPRATTTRNLRESIKGQVGGRTH
                     EIQRLIGRALRSVVDLEKLGERTIMIDCDVIQADGGTRTWSITGAFMALVEALWNYVD
                     RGALSSQGIVKDYLGAISVGVVGGEELLDLAFEEDSAAQVDMNVVMTGSGELVEVQGT
                     AEGDPFSRETLNRLLDLAQKGVGEIVTLEKKLLKRGLP"
     gene            4006..4605
                     /gene="rdgB"
                     /locus_tag="PK016_01710"
     CDS             4006..4605
                     /gene="rdgB"
                     /locus_tag="PK016_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HXL01828.1"
                     /translation="MSGEVYLVTRNEGKLKEAKDILAPFSISVKSIYEICDIGKVEEK
                     GENYAHNALLKARAGFINTGKISLGEDSGLEVDYLRGAPGLYSARFGGENVSSGDKIS
                     LLLKSLQGVPQEERTARFVCVVALVWEGGEEIFRGVCEGYIAEEPRGNTGFGYDPIFI
                     FPPEVKTFAELGSEFKNRYSHRSRAFRQCAEFLIGEVFH"
     gene            complement(4582..5133)
                     /locus_tag="PK016_01715"
     CDS             complement(4582..5133)
                     /locus_tag="PK016_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HXL01829.1"
                     /translation="MEVQIGKCRLIIKQGDITKEDTDTIVNAANSRLAGGGGVDGAIH
                     RAGGPKIAEECRKIGSCPTGRAVITSGGNLPASYVIHAVGPIYRGGKNNEAELLRSAY
                     ISSLELADKYNLRSVAFPSISTGAYGYPIKEASRVALQAIIDYCESHPQTTIEEIRFV
                     LFTPQDKEVYIQSLEELMENFSN"
     gene            5215..6342
                     /locus_tag="PK016_01720"
     CDS             5215..6342
                     /locus_tag="PK016_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HXL01830.1"
                     /translation="MDKVTLVTRVFPEGERVSEVVLEYDSEIDGSNLTPDSYVVEVNV
                     GGKTVERTIKRVYASATPDLFFSTNRGRFVIIELDPQEAMAKTFIFSAETFLSARPEL
                     TYTVAQKVAITTLEGKTIAPFTLQNTDEKHLIADDFQAFVYQDEKLKVEVPYRFFTPR
                     NTDPNKKYPLVMFLHGAGERGRDNFIQLLANKGAVVWAQPGHQALHPSFVLAPQCPPE
                     SSWTGVLSGGEPFEPNLELLSLPHLIEDLVSKYNIDKNRIYVTGLSMGGFGTWAIIME
                     SPQLFAAALPICGGGEVKKVDKIKHIPIWVFHAEDDPLVPVAFSRGLVRSLVEIGGRV
                     KYTEFLKGHLESQGWSAHSSWIPAYEDENVINWLFEKTKPR"
     gene            6375..7304
                     /locus_tag="PK016_01725"
     CDS             6375..7304
                     /locus_tag="PK016_01725"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HXL01831.1"
                     /translation="MKFGASIWPFKWEAPYEDTVVRIANLGFQAVELIAWDREVLDEY
                     YAPGKIKELKDIIMSEGLELSQFVSSPSLMASPVESERRKALEHFKRLVEVGKELGTK
                     IVNSVAPYPFSMDFPRITERPLVQEQIIDIPGDLDWSKNWEDYIEVLRECASICEDAG
                     IQYSLEPHPYRYVSNADSMLRILNQVSSPAIGINFDPSHLFPCGEIPQVVIYRLGKNI
                     IHAHLSDNDGLTNAHWRPGKGKMDWNGILKAFKDVGYDGVLSLELEDVPGVSRSNRFG
                     EAEKISTEILDAEYIKAKEYLKEICQHLNIPVE"
     gene            7329..8438
                     /locus_tag="PK016_01730"
     CDS             7329..8438
                     /locus_tag="PK016_01730"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HXL01832.1"
                     /translation="MAQKVLVYVGTYTSPIKFGTGEILEGKGEGIYVLELNLDTGKLE
                     PKETVTGVLNPSYLVLNGPNSCLYAVNELKEFEGKVSGSVSGFRVSQDGVPAFINKQA
                     TGGSDPCFIEVNASGTYLYVCNFMSGSVCVFPIGADGAVEPYSQFIQHEGSSVNPARQ
                     SGPHPHSFLFSPDGRYALIPDLGLDKVVIYQVDRQTQLLNEASVSYFSTSPGAGPRHI
                     TFDPEGRFMYLINELHSTVVALEYHPENGSLTELQTVSSLPPGVEAAHNTGADIHITP
                     DGVYLYASNRGHDSLACYRVDQKSGLLSYVSCQPSGGKTPRNFALDPTGRYLLCANQD
                     SDNIIVFEINYRTGELREKSRTTIPTPVCVKPYLF"
     gene            8533..9213
                     /locus_tag="PK016_01735"
     CDS             8533..9213
                     /locus_tag="PK016_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HXL01833.1"
                     /translation="MKKWGKFLGGMIFLGVLMMAGQSEGGEKITLPPPCLRSEAKVEE
                     ALWQRRSVRSFLDEALSLGEVSQLLFAAQGVTEKNRGFRTAPSAGALYPLVVYAVVGK
                     VEELSPGVYRYYPQEHALEKMLEGDKREELHRAALSQGAIREAPISLVFTAIYEKTTV
                     KYGERGIRYVHMEAGHAAQNVYLQTEALELGTVAIGAFHDEEVARVLSLPPEEVPLYI
                     MPVGKVKK"
     gene            complement(9800..10402)
                     /locus_tag="PK016_01740"
     CDS             complement(9800..10402)
                     /locus_tag="PK016_01740"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HXL01834.1"
                     /translation="MFFLQREEEVELVINRSRFVGRSLEARSREEVKEKLHEVMELFP
                     GATHYCYAFRIGEDTVEEFSSDAGEPPGSAGRPILGAIRRGDFTDTLVVVVRFFGGKK
                     LGIRGLIDAYGKTAQLVLEKSGKKKHREIEFLVKINPVYFNLVVNQLVEVIQDRRLLS
                     IYPDRGEVQFRISQSEETKIKEILQEGERTGKVLEGRKVK"
     gene            complement(10539..11468)
                     /locus_tag="PK016_01745"
     CDS             complement(10539..11468)
                     /locus_tag="PK016_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXL01835.1"
                     /translation="MPPLKELAKRPERLTSGHRLCAGCGAPIAIRLILAAADKPVVAA
                     NATGCLEVSTSIFPYTAWNIPWIHSAFENAAATITGVETMYRAKRKKGEIDEDVYFVA
                     FGGDGGTYDIGIQSLSGAWERGHRFVYVCYNNEAYMNTGIQRSSATPLGAWTTTSPVG
                     KVLPGKAQWRKNLTEIAVAHDIPYVAQSVPSNWRDLTLKAEKAFHTEGPAFINVLASC
                     NRGWRHLPADTLKVNQLAVDCCIWPLYEVENGQYRLTYKPKEKKPVEEWLKVQGRFQH
                     LFSPANRHLIDEFQAYVDREWERLLQKCEETKK"
     gene            complement(11482..12663)
                     /locus_tag="PK016_01750"
     CDS             complement(11482..12663)
                     /locus_tag="PK016_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HXL01836.1"
                     /translation="MAKVVGFNGNAAVAYAMKQINPDVVAAYPITPQTDCVERFAEYV
                     SEGLVDTEFVTVESEHSAMSACVGAAAAGGRVMTATAANGLAFMWEMLYIAASMRLPI
                     VMMVANRALSGNINIHCDHSDTMGARDAGWIHLFSENAQEAYDNTIQAIKIAEDLEVR
                     LPVMVTQDGFIISHAVERVETLEDEEVKNFIGPYKNRYPLLDLEHPVTYGPIDLFDYY
                     FEHKRSQIEAIDNCLPVIERVGREFGKLSGRKYELLEGYRLEDAEYAVVALGSTCGTA
                     REAIDSLREKGKKVGLLKMRCFRPFPRESVIKALAPLKGVAVLDRSVSFGGFGGPLFT
                     EIRSALYDASSRPLTIDFFYGLGGRDIYPEDIEKALGRIEEVVKAGKVEQHIDYLGLR
                     E"
     gene            complement(12678..12968)
                     /locus_tag="PK016_01755"
     CDS             complement(12678..12968)
                     /locus_tag="PK016_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXL01837.1"
                     /translation="MGELKGWKELVPGGLIVEPGNARDYKTGDWRTEKPELNPEKCIH
                     CFFCWAYCPDNAVIVDAEAGKMLAFNYDYCKGCGICASVCPAKGAITMVAER"
     gene            complement(12961..13536)
                     /locus_tag="PK016_01760"
     CDS             complement(12961..13536)
                     /locus_tag="PK016_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HXL01838.1"
                     /translation="MKKPLEIRWHGRGGQGVVTASRILADATLREGKYFQSFPEYGPE
                     RMGAPVKAFNRISSQPLRMHCGVTNPEIVVVVDPTLLGLAEILEGLDGKGTIVVNFEG
                     SPQELRETLGFKGGTVVTVDASQISRDVLGRVVVNTPMLGALNKVLSEVSMENLLEAV
                     RSQFGEKLRPEVIEKNLLCLTKAYEEAQVDG"
     gene            complement(13542..14162)
                     /gene="recR"
                     /locus_tag="PK016_01765"
     CDS             complement(13542..14162)
                     /gene="recR"
                     /locus_tag="PK016_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HXL01839.1"
                     /translation="MESYPEALSRLISAFSRLPGIGPKTAQRLAFYLVKSPSLEVEEL
                     LSSLQEVKEKIHFCSVCGFYAEGDKCKICESSQRDRSVVCVVERPQDVLAIEKTGFRG
                     LYHVLQGIISPLSGIGPEKLKIKELLERVSQGEIKEVILAISPSVDGEATALYLAQKL
                     SSFPILVTLIARGLPSGGDLEFADEVTLQEALQGRRRISLKPRKEE"
     gene            complement(14178..14489)
                     /locus_tag="PK016_01770"
     CDS             complement(14178..14489)
                     /locus_tag="PK016_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HXL01840.1"
                     /translation="MQNWKNLMKEAQKMQAKIAKMQEELKEKTVEASSGGGMVKVVCN
                     GQQEIINIEIEPELLEEKDVEMLQDLVLAAVNEALRRSREIAEEEMGKITGGLNLPGL
                     M"
     gene            complement(14440..16035)
                     /gene="dnaX"
                     /locus_tag="PK016_01775"
     CDS             complement(14440..16035)
                     /gene="dnaX"
                     /locus_tag="PK016_01775"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HXL01841.1"
                     /translation="MAYLSLYRKWRPSRFEEVVGQEVSVHILTRSLEKKRLHHAYLFC
                     GPRGVGKTTTARLLAMAVNCEKGITSQPCGECESCRSIQEGNSLDVVEIDAASHRGID
                     EIRELRERVKYAPLQSRFKVYIIDEVHMLTTEAFNALLKTLEEPPEHVIFVLATTEPR
                     RLPDTIISRCVKITFNPLPEEAVVEKLKEIAKEEGSTIEEEVFSLIARKVGGSMRDAE
                     GMLEQILAWGGEEAVDLATASLILREIDSEDFTRLLELIIRGNKGKVLLLLNQWIQRG
                     LSPEDISYSLESYLRELLLLCLTQDKREKKKEQWQSLIKGVSEEALQKALQRVSYLEF
                     ELRRSSQPRVLLELCLLDIIKCLAQPELPEENPVQEEVIQKPEGTNGNFWPEILQEVK
                     KAKISLYAFLQAAEFREMGPSHAVLTFNSNCQFHKESVEREENLNILKEVLAKRRGGN
                     WEIECVIDEKVPAVEAGKEREEKKNREEEQFPHNYKSLLGEVMDLFSGVLVDYSLSEE
                     IAKGGWEDAELEKSDEGSPEDAS"
     gene            complement(16075..16340)
                     /gene="ffs"
                     /locus_tag="PK016_01780"
     ncRNA           complement(16075..16340)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK016_01780"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(16355..16449)
                     /locus_tag="PK016_01785"
     tRNA            complement(16355..16449)
                     /locus_tag="PK016_01785"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16413..16415),aa:Ser,seq:gga)
     gene            complement(16724..18622)
                     /locus_tag="PK016_01790"
     CDS             complement(16724..18622)
                     /locus_tag="PK016_01790"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HXL01842.1"
                     /translation="MGKKCISISILVVVSMFLSVAVAAVDNTEIENIIRNAPTQEDYP
                     DAGGVIIKSETVCDFTEVPYRTQVNRICKIFNERGIGEFSEIKIRYDEKREEIELLKA
                     YTIKPDGEVIEVDENAVHDVTPPELTEARIYSHIKDKVINFPGLEPGSIIVCSYLIKE
                     IEPLIEGHFWYTEQYGYTEPIEENSLIVKVPDNKDIYYQTTRGDLEPEIETENGYKKY
                     TWKKLDIPAVTLEVSMPPLIDIVPVVRISTFQSWQEFSDWYSSLITDQYELDGAIRDK
                     INELTANGKLREDKVKALYNYVATNIRYVGLEFGIDGFKPHKSTEVFKNKYGDCKDKA
                     TLLIAMLGEIGVQAEPVLIGTGGYFNLEIASPEYFDHMIVYLPEEDLYLDPTSDVTMF
                     GNIPAYDQGKKVLIPTRNKIVETPLAPAEENCESIKEKVTLAADGSASINISWYDSGV
                     YDFIYKNIFKQYSREQQELVLKQIINSFYPDGDINRCEIKGVEGLEQEFNVEVDLNLK
                     NYAQIMGNVMTFKPFMIGLNVSTVVGPEKRDTPLYIGYKLSAHREIIVDLPEGYVVNF
                     TPESVEIDNEAGTLKSTFNFDEKQMHILLDLKTKMHIVELDKYEQLRDLFNKATENTQ
                     QQVMIGKK"
     gene            19010..21067
                     /locus_tag="PK016_01795"
     CDS             19010..21067
                     /locus_tag="PK016_01795"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXL01843.1"
                     /translation="MLKKYSFSSELIILTTLIFIFTVSVFGYLGYRSSTQHFIMQAFD
                     TMQTIVRLTQQSISQTMEERRIELLLLKKMIGENKLEPYLIDYLLQSFDTLLVLNREG
                     IVIKAWPENQESLIGENLSHREYFQKTQESLKPYLSGYIKSILDTNIISYSIPVIENG
                     EFQGAIVGGMALEQGKIRQFVSSTQFLETGRIIILDQNNNILLSTNSENIGKTFPSLP
                     LKNQSPLIQHIQGEDYLMWMEDIPYSSWKVVASIQEGEILSYSQKTLKLAGILGILIL
                     AVTLSLLYVLLRKLSWPLSHLAFIAKEYAQGNYSLSPLVGGPQEIVQLSDVLNHMRQA
                     IEKRETLLLLEQTYLKTLLQEMSQAVVVLSLEGKLKFANSQLIKLTGYSEEDLIGKNI
                     DELFPSGEKEKIPHLYKWDSSEDKKVERSYLLSSEGKMIPVLLSASPLIIKEEMLGTL
                     MVITNLREIEEKEEELKQALEEIKTLNQELEERNQELEIALAKLDLKLMEADQEKETA
                     QKIAITDSLTQLFNRGFFEKMFPQEFEAAQSNNRPLSLIVADIDLFKKINDTYGHPAG
                     DKVLQDLGKILISSTREEDLVFRFGGEEFIIILHEEKERAQKVAERIRQRVENFSREG
                     KEGPSKFTISLGVASYPKDAQTPRELLIKADQALYQAKKQGRNQVVSYQNSFTSLSPS
                     HIF"
     gene            complement(21074..22546)
                     /locus_tag="PK016_01800"
     CDS             complement(21074..22546)
                     /locus_tag="PK016_01800"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HXL01844.1"
                     /translation="MNAHLVLTIDIGTSSCKVCIFDARGEILKTSKREYPSYSPAPGF
                     AEQNPEEIYQAIKRAVEEVLEDFSPREVEFISFDTMLHSFLLVDRERIPLYPLLNWMD
                     TRCADEVERLKPLYRERKLYFKNAAPLHTIYHIPRVWWFRKNQPHLWEKTSRVLSIKD
                     WILEKITGELFCDYSTASATGLLNLSSFSWDEEILDFAGISLDKLPLLVAPEYTAPLK
                     GGDFTQDTGLLPGTPVIWGGGDGPFANLGDGAFREGEMVVTVGSSGAVRMCSPHPVFD
                     SGERSWCYYLADGIWVGGGAINNGGIVYSWVKDLTGEEEWEWDIHRERPLFLPFLTGE
                     RSPNWNAYARGIIFGLSYFHERAALLQAAFEGVAFRVRSIYDLLQEVMGEPHRIVISG
                     GFASSDKGQRVLCDVLGTKVELSSYPSAPAKGAFLLSCKLRGEVSSLNELPGSFFPSY
                     SIINPDPEAQKFYEEFYQFYKKVYWKNQELFQEFSRFHLA"
     gene            complement(22549..23373)
                     /locus_tag="PK016_01805"
     CDS             complement(22549..23373)
                     /locus_tag="PK016_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL01845.1"
                     /translation="MSYVEELFSIQGKVVLCTGGAGILMSAISEGLGKAEAKIILTDI
                     APLEERLEALKSKGIEAKGYYMDAMDKKSVEEVAQKVKQEFGGIDALLNAAGGNMKDA
                     TTSPEVSFFDLPLEPLQKVVMLNLFGGAIIPSQVFARMMIESGREGNIINFSSMNAFR
                     PLTRIVGYSAAKAAVSNFTQWLAVYLAQEYNPKVRVNALAPGFFITNQNRYLLTKEDG
                     SLTPRGEAVIYHTPLGRFGVPEDLIGTIIWLLSPASSFVTGVVVPIDGGFNAFSGV"
     gene            complement(23399..24904)
                     /locus_tag="PK016_01810"
     CDS             complement(23399..24904)
                     /locus_tag="PK016_01810"
                     /inference="COORDINATES: protein motif:HMM:NF027582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HXL01846.1"
                     /translation="MSDFSKTKEVVKKIGERINLLLRVYSSNYHREELGMVIYPVKGE
                     KERYLGVWSRDKKFYFADPLFLEPQEEKGGFFFLYPFHFKNYLTLSGYFPDLKPQPAG
                     VRTSLGCGDRLGLVSRAHLEAIKDYPVFPVIAQQSPRELQKMERTFQDVLLGAAWGVL
                     ESGNLTPFGADADHLKDEEYLRAGIESGFSMYTLDGSGVASYEMLSKSEKELQKIFDS
                     MPQGEREIFNRYADRTLTLQGGLELDFRKEKFFPLFKVYFPVINFVEKMNFILQERLS
                     SYDLEVSLDEGEGVTSPEVHFFVAEELHRRGIDFKSLAPRFPGSFEKAIDYQGNIQEF
                     SFSLQSHVIVQQEIGGYRLSLHSGSDKFSIYPVFSRETNQLFHLKTSGTSWLKGLEVV
                     AHKDPQLFRKIYELSEEELEENSKAYQISFTKEDIHRDMNTIPDSDLLILFSHPLIRQ
                     FLHISYGSVWRAFKSELSHLFFQYEEEHYRLVRENMERHLRFLFSLEENIE"
     gene            complement(25195..26598)
                     /gene="aglA"
                     /locus_tag="PK016_01815"
     CDS             complement(25195..26598)
                     /gene="aglA"
                     /locus_tag="PK016_01815"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032450 - maltose alpha-glucosidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase AglA"
                     /protein_id="HXL01847.1"
                     /translation="MKLVKISFIGAGSIRYTLALVGDLAKTKELSGSLISLMDVDEER
                     LEAVYNLAKRYTDEVGGNLKLEKTTDREESLQGADFVINTALYRAPGHEDGYVSYEIM
                     RDVAEKYGYYRGIDSQEFNMVSDYYTFTNYNHLKLSLDIAKSVEKICPDAYFIQTANP
                     VFEITQLILRQTTVKAIGFCHGFSGLFAVFHVLGLNPEEVDWQVAGVNHGIWLNRFLY
                     RGENAYPLLDRWIEEKSQYWQPENPWDVHLSPAVVDMYRFYGLLPVGDTCRNGTWKYN
                     YNLDAKKKWHGKFGGIDNEIERPRFYEELRQRKRMIIELSKDSGIKLTKTWPEEFSMD
                     KMSGEQQVPFINAIVNNKATRLVLNILNQGVVKGISDDVVVEIPLEVDESGIHPERIE
                     PPLPDKVIKYYLTPRITRMEMAIDAFTTGDRKILEELLVRDPRTKNYEDISRLWDEIF
                     SLPFNQEMKVHYSIPQN"
     gene            complement(26759..27760)
                     /locus_tag="PK016_01820"
     CDS             complement(26759..27760)
                     /locus_tag="PK016_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HXL01848.1"
                     /translation="MKITINELARRSGVSPSTVSRALNDDPVISEETKRKVMQVAKEL
                     NYTPVRSRKRSSTRKRSRIIALMVTDISNPFFPEIVHGVEDLAFDSGYSVSLWNTRED
                     MEREKQYVETLKVSEVDGIILGSSRIGEEQIREVVNSDIPCVIINRIIEGIPSVFADY
                     ESGAYQATKYLLSLGHERIALINGPNSAQPSLWREKGFLRALKESGREVKKGLLSFNL
                     PVVEGGYVATLKLLSIDNPPSAIFAYNDLVALGAMKAIREKNLLISKDISLIGYDDIF
                     LSAYLDPPLTTVNQPKYMMGKLAADLLFRLINGEKVKNNQISLKPELVIRSSCGVRV"
     gene            complement(28126..29388)
                     /locus_tag="PK016_01825"
     CDS             complement(28126..29388)
                     /locus_tag="PK016_01825"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HXL01849.1"
                     /translation="MSEQGHSYANMLKVLDASAEKLGVCWDDYVPLRYPERELTVSIP
                     VEMDDGHVKVFTGYRVQHSSVRGPCKGGLRYSSDVDLDEVRTLAALMTWKCAVVNIPY
                     GGAKGGVQCDPGKLSSRELMRVTRRYTAMILPLIGPEKDIPAPDVNTNAQIMGWIMDT
                     YSVFKGFTVPGVVTGKPIEIGGSLGREDATGRGVLFTINQIALQKGIDLSKASFAIQG
                     FGKVGEAIARLLYQEGYRVVAISDISGGMYKKEGLDIMDISNYVKKNPSHLIEGYEAE
                     GISSLTNQELLTLEVDILVPSARENQITKDNARDIKAKIIVEAANGPTTLEADQILSE
                     RGITVVPDILSNAGGVVVSYLEWVQNIQSLMWDEEKVNTFLQKIMERSFQEVWEFQEQ
                     NRVPLREAAYMLALDRTIKARKIRGNFP"
     gene            complement(29997..30146)
                     /locus_tag="PK016_01830"
     CDS             complement(29997..30146)
                     /locus_tag="PK016_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01850.1"
                     /translation="MVYFKMGSIIMLAKLPELTEKDIGKEVNLSVDFSRLHIFDKKTG
                     ERIEF"
BASE COUNT         8862 a         6768 c         5751 g         8852 t
ORIGIN      
        1 agtgatagga agattacttc tttctatagt caatttatag tatcccgcaa aactgattaa
       61 aaactttagg aagtaaatta tatcgaaagt caaaaactca aggtcgctac gctggggtgt
      121 tttctccagc gtaattatca agattttttc ttatctaatt tgctttaagt attacgccgt
      181 aggctgtcta ctttttgggg gttataatgg ggacaagctc tcttatagcc ttcaccttca
      241 ccctcaccct aaccctctcc cttcgagggc gagggaagga atatgtgggg ttcttccatt
      301 gaaggcgagg gaagaaaaag aaaggggatt cataaggggg aattctttcc cccttatagt
      361 ttactattag gtatcagata aggctctcaa taacggttaa ttataactga ttttacagtt
      421 aactataaat aaaaaagcct tttctctatt taagaatggg aaagaatatg ttaaactcat
      481 atagaaaata atttttgatg aaataggggg atgttttctg atggctaggg acatgaattg
      541 tttttttggg atcgggaatt taactcggga acccgatttg cgttataccc cccagggaac
      601 accagtttgt cgattcagta tcgctatgaa tcgtagttat tcaggtaaac aggggatggt
      661 gaaagaagtt acctatgtca ctgtggctgc ctggtctaaa ctggcagaga tttgtgctcg
      721 ttatctccac aaaggcagtc gggtggcggt gatgggggag ctgcgttcta attcctggga
      781 agaccgcaat ggcagtcgtc gtgtctccta tgaaattaga gcagataaca tccagttttt
      841 gagccccccg aaaggagtaa cgcaagaaga agtagaagag tttaccggat tgggagcaaa
      901 attggaaatg gaggaggccg aaccgtttgt ctatgaaagc ggagaaaacc cactgggaga
      961 gctggaagat atccccctgg agaaagacga cgggggagaa attgcccctt tttaggagaa
     1021 aagtttttgt tctttttttg ttcttttttc ttctccccct ttttgctctg gaagcacagg
     1081 ttaagataga ctggggagaa gagtcttttt actctcaggc agaagtgaga gttcgccagg
     1141 gtaaaactta tgtaaatgta gaagagctgt ttcgggaaat agggggaatt gtttattatt
     1201 cccccattat gaataaaata aacttaggtt ttaaaggtac agcctgggtt ctttctctag
     1261 ataagggagt ggcggtacat aatggaagag aagtttcctt agaaggggaa gtttttgtag
     1321 aaggcaatgc tcctttcgtc tctttagatc ttttaagtca gcttttttcc tttgttcctt
     1381 ctactcccac tacttttgct accccgcctc cggaggtaac tttttcccct cctcctgggg
     1441 aggaaagata ttcttctttt gtccttttag gaatccggtt ttactcttat tcggaggaga
     1501 tgagaaccag agttaccttt gattttcaag ggggaatccc ctcttattct tatcgtatgg
     1561 accgcgagga gaatagaata actgttgagc tttcaggttg tgactggcga caatctatgt
     1621 ctcctattat agtgaatgat gggcgaataa accgtataga attaacaaaa actgccgagg
     1681 ggttaagagt taatatctgg ttacagaatc cagtacaggt taaagaggaa aagaagctgc
     1741 ctggggataa tcctcgaatc tactttgacc tggtttctct ggtggaggct aaagtggaag
     1801 catcgccaga agtaaaacca actacaactc ctgtagcttc acctaaaatt cagcctactc
     1861 ctacctctcc gcaagtaact cctggtctct cctataatga agttaacacc aaagtggtgg
     1921 ttatagatcc cggccatgga ggcaacgacc ctggctgtgt atacaatggc tataaagaaa
     1981 aagatatagt gttagctatt tctcaaagag taaaggtggc cttagaaagc aaaggttatc
     2041 aggtttttct gactcgttct caagacgttt atccttcttt gaatgaccga tatcgagtgg
     2101 ccaatgaacg gcggcctttc gttttcttaa gcattcactg caatgcttct cctaatcccc
     2161 aggccacagg tgtagaggtc tttgtgggag gagtgaaacc ccgaggagaa ggagcaggag
     2221 atgtggccag tcgggaaaat caactattcc tggaagaagg aaaaacagaa gatattaacc
     2281 gggtgggcaa tatcttatct tctgcctatt accagagcag tcgagaagtc agcgccgagc
     2341 tgggtaaaat aatgtcggaa aaaatttctt ctactacggg acagaaaaac cggggattga
     2401 aagaagcacc tcttgtggtc ctggacggca tgtattatcc agcttgtttg gttgaagtgg
     2461 gcttcctgag caacgctgaa gaagccaaaa agttagcctc ttcttctttt caggaaaaaa
     2521 tagctcaagc tattgctcag gcagtagacg attttagccg aaaccctaaa ttcaagagct
     2581 ttttaggaga atagaatgaa aaatcaaact cccccttctc gaaagaaata taaagaaaaa
     2641 gaatcttcct ggccaggttg gttttatatc cttgtaggtt tagccatttt ctttgccgtt
     2701 ctttactttg gagagttaat tataagatat ttacaacctc cagaaatttc tccttctcct
     2761 ggggttgtcg tttctccgga ggaggaacgg gaagagatat tacctgctgg ggaagaagag
     2821 gtggtccttt acttttccaa tcctgatttt accgctttag taggagaaaa aagaaagata
     2881 aaaaaagata gtaaatggat ggagagaata atagaagagc tattaaaagg gccgcgggat
     2941 cgctctttgt acaaccccat acctccctcc actcgtttaa atgctgtgtt tttagaagga
     3001 gatattgctt atgtagattt gagcgcagag atggctaaaa accaatcagg aggcacttcc
     3061 caggagctct taagtgttta tgccatcatt gatactttaa ctgcttttcc agaaataaag
     3121 aaagttaaaa tattaatcga cggggaagaa atccctactc tgtgtggtca tatagatatt
     3181 tcccaacctt tagaaaggga tgaaaaatta attgccaaag ttcaataatt tccaagaatt
     3241 gggcgacttt tattttaaaa aggataaagc aacctggaag ggaaggccag atcgccgtcg
     3301 gcctgatgag ttaagaccgg tggagataca tcttcattat ctccgttatg ctgaaggttc
     3361 agttttagta gaggcgggca acaaccgcat agtttgtgct gcttccatag aagacaaagt
     3421 cccccctttt ttgcgtaaca gcggtcaggg ttggattact gcagaatacg ctatgctccc
     3481 tcgggccact accactcgta acctgagaga atcgataaaa gggcaggtag gaggtagaac
     3541 tcacgaaatt cagagattga taggtagagc cttacgctca gtagttgatt tagagaaact
     3601 gggagagaga accattatga ttgattgtga tgttattcag gctgatggag gtacaagaac
     3661 ttggtctata accggcgctt ttatggcttt agtcgaagct ctgtggaatt atgtagaccg
     3721 gggggcatta tcttctcaag gtatagtaaa agattattta ggagccatca gcgtgggagt
     3781 agtgggaggg gaagagctct tagatctcgc ttttgaagaa gattctgctg ctcaagtgga
     3841 catgaatgta gtgatgaccg gtagcggaga gctggtggaa gtgcagggta cagcagaagg
     3901 agaccctttt tcccgagaaa cgctaaatcg tcttttagat ttagctcaaa aaggggttgg
     3961 tgaaattgtt actttggaga aaaagctttt aaaaagaggt ctaccttgag tggcgaagta
     4021 tatttagtca cgagaaatga ggggaaacta aaggaggcaa aagatatcct cgcccctttt
     4081 tccatttcgg taaaaagtat ttatgaaatt tgcgatatag gaaaagtaga agaaaaaggg
     4141 gaaaactacg ctcataatgc ccttcttaaa gctcgagcag gatttataaa tacgggtaaa
     4201 atctctttag gagaagactc aggattagaa gtagactacc ttcgtggagc accgggctta
     4261 tactcagctc gcttcggggg agagaatgtc tcttccggtg ataaaatctc tctgcttttg
     4321 aaatctctgc aaggagtccc gcaagaagaa cgtaccgctc gctttgtctg tgtagtggct
     4381 ctggtatggg aaggaggaga agagatattt cgaggagtat gcgagggcta catcgccgaa
     4441 gagccccgag gaaacacagg atttggttat gaccccattt ttatctttcc tcccgaggta
     4501 aagacttttg ccgagttagg ttctgaattt aaaaatcgtt acagccaccg ctctcgagct
     4561 ttccgtcagt gcgctgaatt tttaattgga gaagttttcc attaattctt ctaagctctg
     4621 aatatagact tccttatcct gaggagtgaa aagcacaaag cgaatctcct ctatggtagt
     4681 ttgggggtga ctctcacagt agtcaattat agcctgcaag gctacgcgag aggcttcttt
     4741 tatgggataa ccgtaggcac cggtggatat agaagggaaa gccacagagc gcaggttata
     4801 cttatcagct aattcgagac tggagatata ggcactacgc aggagctcag cttcgttatt
     4861 ttttcctccc cggtaaatgg gccccacagc atgaatgaca tatgaggcag gtaaatttcc
     4921 cccactagtt attactgctc gtcccgtagg gcaacttcct atcttccggc attcttcggc
     4981 aatcttcggg cctcctgctc ggtgaatagc tccatctacc cctccccctc ctgccaggcg
     5041 agaattggcg gcgttaacta tggtatcggt atcctctttg gttatgtctc cctgcttgat
     5101 tatcaagcga cacttcccta tctggacttc cattttccca cctctttttt ccattatatc
     5161 acgagaaaag aagtagggtg atataatgga aaaaactgaa agggaggaaa agaaatggac
     5221 aaagttactt tagttaccag agtttttccc gagggagaaa gagtgagcga agtggttttg
     5281 gaatatgatt ccgaaatcga tggaagcaat ctcactcccg atagctatgt ggtggaggta
     5341 aatgtgggag gtaagacggt cgagagaact attaaaagag tatacgccag cgccacccct
     5401 gatttgtttt tttccaccaa tcgagggaga tttgtgatta tagagttaga cccccaagag
     5461 gcaatggcta aaacttttat tttttctgcg gagacttttt tatcagcaag accagagcta
     5521 acctatactg tggcccagaa agtagctatt acgactctcg agggaaagac catcgctcct
     5581 tttactcttc aaaacaccga cgaaaagcac ctcatcgccg atgactttca agcttttgta
     5641 tatcaggatg agaaactgaa agtggaagtt ccttaccggt tctttacccc ccggaacacc
     5701 gatccaaata aaaaatatcc tctagtcatg tttctacacg gagcaggaga aaggggaaga
     5761 gacaacttca tccagttgtt agccaataag ggggcggtgg tctgggctca acctggtcat
     5821 caggctctgc acccttcttt tgtattagct ccccaatgcc ccccggagag tagctggact
     5881 ggagtgcttt caggaggcga accttttgaa cccaatcttg agctactttc tcttcctcat
     5941 ctgattgaag atctggtttc taaatataat atagataaga atagaatata tgtcaccgga
     6001 ttatctatgg gagggtttgg aacctgggca ataataatgg aaagccctca gctttttgcc
     6061 gcagcccttc ctatatgcgg gggaggggag gtaaaaaagg tagataaaat taaacatatt
     6121 cctatctggg tctttcatgc cgaagatgac cccttggttc cggtcgcttt ttctcgaggt
     6181 ttggtgagga gtctggtgga aataggaggg agagtaaaat acactgaatt tctcaaaggc
     6241 catctggaat ctcaagggtg gagcgctcac tcttcttgga ttcccgctta cgaagatgaa
     6301 aatgtgatta actggctgtt tgagaagacg aagccccggt agtctaaaaa ataagagaaa
     6361 gggaggataa aactatgaag tttggagcat cgatctggcc gttcaaatgg gaagcaccgt
     6421 atgaggatac ggtagttaga atagcaaatt taggtttcca agccgtggag ctaattgctt
     6481 gggataggga agtgctggat gaatattatg ctccagggaa aataaaagaa ttgaaagaca
     6541 taataatgag cgagggattg gaattatcac aatttgtttc cagccccagt ctaatggcct
     6601 ctccagttga aagcgagaga agaaaggcat tagagcactt taagagactg gtagaggtgg
     6661 ggaaagagct gggaacaaaa attgtaaatt ctgtggcacc ttaccctttt agcatggatt
     6721 ttccccgaat cacagaaagg cctctagtac aagaacaaat catagatatt ccaggtgatt
     6781 tagattggtc gaaaaattgg gaggattaca tcgaggtctt gagagaatgt gcatcaattt
     6841 gtgaagatgc agggatacag tattcactgg agcctcatcc ttaccgatat gtgagcaatg
     6901 cggattcaat gttgagaatc ttaaaccagg tgagctctcc cgctatcgga ataaactttg
     6961 accccagtca cctattcccc tgtggggaaa taccccaggt agtcatatac agattgggca
     7021 agaatatcat tcatgcccac ctttctgata acgacggatt gaccaacgct cattggagac
     7081 cagggaaagg aaaaatggac tggaacggga ttctcaaggc gtttaaagat gttggctatg
     7141 atggagtgtt atctctggaa ctggaagatg tgcccggggt ttccaggtca aacaggttcg
     7201 gggaagcaga gaaaatatct acagaaatac tggatgcaga atacatcaaa gccaaggaat
     7261 atctaaaaga gatatgccag cacttaaaca ttccagttga ataaggattg ccaaggagga
     7321 attaaactat ggcacagaaa gttctcgtgt atgtgggcac ttatacctct cccatcaagt
     7381 ttggaacagg agagatattg gaaggcaagg gagagggcat ttatgtattg gaattaaatc
     7441 ttgatacagg aaagcttgag ccaaaggaga ctgttaccgg ggttttaaat ccttcttatt
     7501 tggtgttgaa tggccccaat agctgcctct atgctgtaaa cgaattgaaa gagtttgagg
     7561 gaaaggtttc gggctccgtc agcggtttta gagtcagcca ggatggtgta ccagccttca
     7621 tcaataaaca ggctacgggt ggttctgacc cctgttttat agaggtgaac gcttccggca
     7681 cttatcttta tgtatgcaat tttatgagtg gaagtgtatg cgtttttccc attggtgctg
     7741 acggagcggt ggagccctat tctcagttca tccaacatga aggtagcagc gttaatccgg
     7801 caaggcaaag cggacctcac cctcactctt tccttttttc accagatggt cggtatgctt
     7861 tgatacccga tttaggtttg gataaagttg tgatttacca ggtagatcgc caaacccagc
     7921 tactgaatga ggcttctgtt tcctattttt caacctctcc cggtgctgga cctcgccata
     7981 ttacttttga cccggagggg aggtttatgt atctcataaa tgaactacat tctactgtgg
     8041 ttgcccttga gtatcatccc gaaaatggta gtctcaccga gttgcaaact gtctcttcgc
     8101 tccctcccgg ggttgaagct gcccacaata ccggtgcaga tattcacatt acgcctgatg
     8161 gtgtctactt atacgcttcc aaccgagggc atgatagtct ggcctgttat cgggtagacc
     8221 agaaatcagg gttactctcc tacgttagct gtcagccttc aggaggcaag acccctcgca
     8281 actttgccct tgaccccacc ggacgttatc tattgtgtgc taaccaggac agcgataata
     8341 ttatagtttt tgagattaac tatcgcacag gggagctcag agaaaaatcg agaacaacga
     8401 ttcccactcc tgtttgtgtg aagccttatc ttttttgatt tcctgtccta tatgatacga
     8461 gatggaatgg tattattagc ttaaaatagg ataaaggagt ttactctaat attaaaaaag
     8521 gaggggagag atgtgaaaaa gtggggaaaa tttctgggag gaatgatatt tttgggggtg
     8581 ttgatgatgg cagggcaaag tgaaggagga gagaagatta cccttccccc accttgcctg
     8641 agaagcgagg ctaaagtaga agaggctctc tggcaaaggc gctccgtacg ctcttttctc
     8701 gatgaggcgc tatctttggg tgaagtttct caacttctct ttgctgctca gggggtaaca
     8761 gagaaaaatc ggggctttcg tactgcacca tcagcagggg cactttatcc tctggtggtt
     8821 tatgcggtag tggggaaagt agaagaacta agccccgggg tttatcgtta ttatcctcaa
     8881 gaacatgctc tagagaaaat gctggaagga gataaaagag aagagcttca ccgcgctgct
     8941 ctttctcagg gagctatccg ggaagctcca atatctttag tttttaccgc aatttatgag
     9001 aaaaccactg taaagtatgg agagcgaggg attcgctatg tccacatgga ggcaggacac
     9061 gcagcacaaa atgtctacct ccagactgaa gcgctggaat taggaacggt agctatcggt
     9121 gcttttcatg atgaggaagt tgcccgggtg ctttctctgc ctcctgagga ggtgcctctc
     9181 tacatcatgc cggtggggaa agtgaaaaag taaaaaagta aatataactc tgccgataat
     9241 ttttgaaagg cgagatttat gatatacgga gatagtcggt cccgtttttc aatatggtca
     9301 aggagcagca tttatttatc cttttctttt atcttttcag taaccaggaa acccattcca
     9361 caaactggtt cctgtgcttc aaaatagaaa aaatatagat ttttcaagag ctctaaatgc
     9421 caggaaggct aaaaagatga agaaactggc aaatctggga aaacaattat tgttttatag
     9481 ttaaccgcaa aatccattat aaagactatg agggggttta ccccctcata acccccaaaa
     9541 agcaaaagaa aagaacaaag acttaaaaag caaataaaca aaaactactg gtggaaaaat
     9601 atccaccagt atgctggaga aaaaggcagt ctgcgacgca agattaagaa aacttaaaaa
     9661 acaaaagaaa taaaggccct agagaaagac tttgtctttc tctatggcat agcgaccttg
     9721 agtttttgac tttcgatata atttactttc taaagttttt aaccagtttt gcgggatact
     9781 ataatcattc aagcaatttt tattttactt ttcttccttc taaaactttc cctgttcttt
     9841 ctccttcctg gaggatttct tttatttttg tttcctcact ctgagaaata cgaaattgta
     9901 cctctcctct atcaggataa atcgagagaa gccgcctatc ttgaataacc tctaccagtt
     9961 gatttaccac caggttaaaa taaacagggt taattttaac caaaaattct atttctctgt
    10021 gctttttttt acccgacttt tctaaaacca gctgagcagt ttttccataa gcgtcgatta
    10081 gtcctcttat ccccaatttt ttacccccga agaaacgcac cactactacc agggtatcgg
    10141 taaaatcgcc tcgccggata gcacctaata taggtctgcc tgcacttccc ggtggctctc
    10201 ctgcatcgga agaaaattct tctaccgtat cttctcctat ccggaaagcg taacaataat
    10261 gagtagcacc aggaaaaagc tccattacct catgcaactt ctcttttacc tcttcccgag
    10321 agcgcgcttc cagacttctc cccacaaatc gagagcgatt tatgactaat tctacttcct
    10381 cttcccgctg aagaaaaaac atcttatcaa ctacccagat aataatttat aaaattcaag
    10441 agagcagaat aatctttgac ttttcgaatt ccaagtggcc tgagagaata aagaggagct
    10501 agagacctga aatctctagc tcctctcggg gtaaagaact actttttagt ttcctcgcac
    10561 ttttgcaaaa gcctttccca ttcccggtct acataggcct ggaattcatc gatcagatgg
    10621 cggttagcag gggaaaagag atgttgaaat cggccctgca ctttcaacca ttcctctacc
    10681 ggctttttct ctttaggttt ataagtaagt cggtactgac cattttctac ctcataaaga
    10741 ggccagatac agcaatctac tgctaattgg ttcactttta gggtatcagc agggagatgc
    10801 cgccatcccc gattgcaaga agccagcaca ttaataaagg ctggtccttc agtgtgaaaa
    10861 gctttttctg ctttaagggt taaatctcgc cagttagaag gaaccgattg agccacataa
    10921 ggaatatcgt gagctacggc aatttcagtt aaattttttc tccactgagc ttttccgggg
    10981 agaaccttac ccaccgggct tgtggtagtc caggctccca ggggagtagc actggagcgc
    11041 tgaataccag tgttcatata agcttcgtta ttgtagcata catatacaaa ccgatgtcct
    11101 ctctcccagg ctccagaaag agattgtatg ccaatatcat aagttccacc atcaccgccg
    11161 aaggctacaa aatagacatc ttcatcgatt tccccttttt tcctcttcgc tcgatacata
    11221 gtctctactc cggtgatggt agcggctgca ttttcaaaag cgctatgaat ccagggaatg
    11281 ttccaggcag tgtaagggaa aatactggta gatacctcta aacaccccgt agcattggca
    11341 gcaacaactg gcttatcagc agcagccaga atcagtcgga tagcaatagg agctccacat
    11401 cctgcacaca gtctgtgccc agaagtaagc ctctctggtc ttttagctaa ttcttttaga
    11461 ggtggcattt tctaaaacct cctattctcg taaacctaaa taatcaatat gctgttctac
    11521 ctttcctgct tttaccactt cctctattct ccctaaagct ttttctatat cttcaggata
    11581 aatgtccctt cctcccagac catagaagaa gtctatggtt aaaggacgag aagaagcatc
    11641 gtaaagagcg gaacgaattt ctgtaaatag tggtccacca aatcctccaa aggaaacaga
    11701 gcggtctaaa actgctaccc ccttcaaagg agcaagagct ttaatcacac tttctcgagg
    11761 gaagggacga aaacagcgca tcttgagaag ccctactttt ttccctttct cccgcagaga
    11821 atcaatagct tcccgggcag ttccacaagt agaacccaga gccaccactg catattcagc
    11881 atcttctaat cgataacctt ccagaagttc gtactttctc ccggaaagtt ttccaaactc
    11941 cctgcccact cgctcaatta ccggtaagca gttatcaata gcctctattt gagacctttt
    12001 gtgttcaaaa taatagtcaa aaaggtcaat aggtccgtag gtaacaggat gctctaaatc
    12061 gaggagagga tagcgatttt tgtaaggacc aataaaattc ttcacttcct catcttctaa
    12121 agtttctact ctctctacag catgactgat gataaatcca tcctgagtaa ccataactgg
    12181 aagtcttact tctaaatcct cggcaatttt aattgcctga atggtgttat cataagcttc
    12241 ttgagcattc tccgaaaaaa gatggatcca tcccgcatct cttgctccca tagtatcaga
    12301 atggtcacag tgaatattga tattaccaga taaagctcgg ttagctacca tcatcacgat
    12361 gggaagacgc atactggcag caatatagag catttcccac ataaaagcca aaccattagc
    12421 agcagtggca gtcatcactc ttcctccagc agctgctgct cccacacaag cactcatagc
    12481 gctatgttca ctttctactg tgacaaattc agtgtctact aatccttcac tgacatactc
    12541 agcaaatctc tccacacaat cggtctgggg agtaatagga taagctgcca cgacatcagg
    12601 gttaatctgt ttcatagcat aagccactgc cgcattgccg ttaaaaccca caactttagc
    12661 catttccttc acctccctta ccgctccgct accatagtaa tggctccctt agcgggacaa
    12721 acgctggcgc atattccaca tcctttacag taatcgtagt taaaagccag cattttccct
    12781 gcctcagcat ccactatcac tgcattatcc gggcaataag cccagcaaaa gaaacagtga
    12841 atacattttt ccgggttaag ctccggtttt tcagttcgcc aatctccggt cttataatct
    12901 ctcgcattac ctggttccac aattaaccca ccgggcacca attctttcca tcctttgagt
    12961 tcacccatct acttgtgcct cctcataagc tttagtaaga caaagcaagt ttttttctat
    13021 aacttctggt ctcagttttt ctccaaactg ggaacgaaca gcctctaaga ggttctccat
    13081 gcttacttct gaaagcacct tatttagagc acccagcata ggggtgttaa ctaccactct
    13141 ccccaggaca tctcgagaaa tctgagaggc atctaccgta accactgttc ctcctttaaa
    13201 tcctaatgtt tctcgtagtt cttgaggaga accctcaaaa ttgaccacta tagttccttt
    13261 tccatccaac ccttctaaaa tttcagctaa acctaaaaga gtaggatcta ctaccaccac
    13321 tatttctgga ttagttactc cacagtgcat acgaagcggt tgagagctga tacgattaaa
    13381 agcctttacc ggagctccca ttctctccgg gccatactcg ggaaaagatt gaaaatattt
    13441 tccctctcgt aaagtcgcat cagctaaaat tcgagaggca gtaactactc cttgcccacc
    13501 tcgaccgtgc cacctgattt ctaagggctt tttcaagatt gttattcctc ctttctcggt
    13561 tttaggctaa ttcttcttct accttgtaaa gcttcttgca aagttacttc atcagcaaac
    13621 tccaggtctc caccagaagg caatcctcga gcgataagag tcacgagaat gggaaacgat
    13681 gataattttt gcgctaaata aagagcagtc gcttccccat ccaccgaagg actgatagct
    13741 aagatcacct cctttatttc cccttggctc actctttcta acaattcctt aattttgagc
    13801 ttttctggac cgatgccaga aagaggagaa ataataccct gcagaacatg atacaacccc
    13861 cgaaatcctg ttttctctat agccagaaca tcttggggtc tttctacgac gcaaactaca
    13921 cttcggtctc tctgggaact ttcacaaatt ttacatttat ccccttcagc ataaaacccg
    13981 cagaccgagc aaaaatggat tttttctttt acctcctgca gggaagacaa taattcttct
    14041 acctctaaag aaggagattt aaccagataa aaagccaagc gctgagcagt tttgggcccg
    14101 ataccgggca aacgagaaaa agcggaaatc agccttgaca gagcttcagg ataagattct
    14161 atcaacgatg tccctcttta cattaaacca ggaaggttta atcccccggt aattttaccc
    14221 atctcttctt cagctatttc tcgtgaacgg cgcaaagcct cgtttactgc cgctaaaact
    14281 aaatcttgca gcatttccac gtctttttct tccagtagtt cgggctcaat ttcaatattg
    14341 attatctctt gctgtccgtt acaaactact tttaccatcc caccaccaga ggaggcttcc
    14401 accgtttttt ctttcaactc ctcctgcatt ttggctattt tagcttgcat cttctgggct
    14461 tccttcatca gatttttcca gttctgcatc ttcccaccca cctttcgcaa tttcctcact
    14521 taaggaatag tcaaccagta ccccggagaa aaggtccata acctcaccaa gcaatgactt
    14581 ataattatga ggaaactgtt cctcttccct gtttttcttt tcctccctct cctttcctgc
    14641 ttctactgca ggaaccttct catctataac acattctatt tcccaattcc ccccccgtct
    14701 tttagctaaa acttccttaa ggatgttcaa gttctcttcc ctctcaacac tctctttgtg
    14761 aaattgacaa ttagaattaa aagttaaaac agcatgagaa ggacccattt ctctaaattc
    14821 agctgcctgc aaaaaagcat aaagagaaat tttagctttt ttcacctctt gtaaaatctc
    14881 tggccagaaa ttaccattag tcccttccgg tttctggata acttcctcct gtaccggatt
    14941 ttcttcaggt aattcaggtt gtgccaagca cttaattata tccaataaac aaagttctaa
    15001 caacacccgg ggttgtgaag aacgacggag ctcaaattcc agatagctta ctctctgtaa
    15061 agctttttgc agagcttctt cgctgactcc tttaattaaa gattgccact gctctttttt
    15121 cttttccctc ttgtcttgag taaggcataa tagtagcaat tcccgcagat aactttccag
    15181 gctatagctg atatcctccg ggcttagccc tctttgaatc cactgattaa gaagtaaaag
    15241 cacttttccc ttattccccc gaattatgag ctccaaaagc cgggtaaaat cctcactatc
    15301 tatttctctt aaaatcaagg aagcagtagc taaatctaca gcctcctctc ctccccaggc
    15361 taaaatttgt tccagcatcc cttcggcatc tcgcatagaa cctcccactt tacgagctat
    15421 gagagagaaa acttcctctt caatagtact tccttcttct ttagctatct cttttaactt
    15481 ttccaccaca gcttcttccg gtagcggatt aaaagtgatt tttacacagc gagaaatgat
    15541 ggtatcggga aggcggcgag gctcagtagt agccagcaca aatataacat gctctggcgg
    15601 ctcttccagg gttttgagca aagcgttaaa agcttcggtg gtaagcatat gcacttcatc
    15661 aattatgtaa actttgaagc gagattgtaa aggagcatat ttaactctct cccggagctc
    15721 tcgtatttca tctatacccc ggtgagaagc agcgtcaatt tctaccacat ccagagaatt
    15781 tccctcttgg atggaccggc aactttcgca ctccccacaa ggctgtgagg ttatcccctt
    15841 ctcgcagtta actgccatag ccagaaggcg agcagtagta gtttttccta ctcctcgtgg
    15901 tccacaaaaa aggtaagcat gatgaagtcg ttttttttcc aaggaacggg tcaaaatatg
    15961 taccgatacc tcttgtccca ctacctcttc aaaacgagac ggtctccact ttctatagag
    16021 tgacaggtaa gccaacgttc taccttctta tttttttcaa taaattgcct ctaatcgtac
    16081 accgacattc gataatttct tcccggtcgt agcagaggca gtaaactccg gttaggcgct
    16141 cttgcggcac ccgaagagct tcacgtaccg ctgctccctc ccaggcctga cggggttggt
    16201 gaagattcgt cgcgcaagac ccaaccttca tcgttccgtt cctccacccg gaaccgaaaa
    16261 gaaagaacct cctgtcggaa ttcagccctg ctgtagcgga ttgcaggtac agggcaccgc
    16321 tatctccccg cttagcacga ttagaggcaa aatctggcgg agagggtggg atttgaaccc
    16381 acgagacagg ttcgacgcct gtctacccgc tctccaggcg ggcgccttcg tccactcagc
    16441 cacctctcct tccaagaata attataaagg gaaagggaaa gttttacaag tttatctcac
    16501 cgaatgacga gtccttttaa ttttaagacg agaaaaacag attagattaa atcataatat
    16561 atacctctgg aaatacttta ttatggataa gacaccaacc accaaagttc atattttctc
    16621 cgggagatag ctttccacct cagtatctgt cactcccgga gattacattt ctaattctga
    16681 aaatatctct cacccatagg ttaaattcag gcatttcaat accttatttt ttcccgatca
    16741 ttacttgctg ctgcgtgttt tcggtagctt tattaaataa atcacgcaat tgttcatatt
    16801 tatcaagctc tacaatatgc attttagtct ttaagtccag taatatatgc atttgttttt
    16861 catcaaaatt aaaagtactt tttaaggttc cagcttcgtt atctatttcc acactttcgg
    16921 gcgtaaagtt aacgacataa ccttcaggca aatcaacaat tatctcccgg tgagcactta
    16981 gtttataacc tatatagaga ggagtgtctc ttttttcagg tcctactact gtacttacgt
    17041 tcaacccgat cataaaaggc ttaaaagtca tgacattacc cattatctga gcataatttt
    17101 taagatttaa atcaacttca acattaaatt cctgttctaa gccctcaact ccttttattt
    17161 cacatctatt aatatctccg tcaggataaa atgaattaat aatttgtttt aaaactaatt
    17221 cttgctgttc tctgctatat tgtttaaata tatttttata gataaaatca tataccccgc
    17281 tatcatacca gctgatatta atactggctg aaccatcagc agctaacgtt actttttctt
    17341 ttatactctc acagttttct tcagcaggag caagtggcgt ctctacaatt ttatttctcg
    17401 tcgggattaa tactttctta ccctggtcat acgccggtat attcccaaac atagttacat
    17461 cactagtggg gtcgagatat aaatcttcct ccggcagata aacaatcatg tggtcaaaat
    17521 attcgggact ggcaatttcc agattaaaat agcctcctgt accaattaaa acaggttctg
    17581 cctgaacacc aatctcaccc aacatagcaa tcagtaaagt agccttgtct ttacaatctc
    17641 catacttatt tttaaacact tctgtagatt tatgcggttt aaaaccatct ataccaaatt
    17701 ctaaccctac gtagcgaata ttagtagcta catagttata aagggcctta accttatctt
    17761 cacgcaactt accatttgct gtcaattcgt ttattttatc ccttattgcg ccatctaact
    17821 cgtactggtc tgtaataaga cttgaatacc agtcactgaa ttcctgccat gattgaaatg
    17881 tagaaatcct tactacaggt actatgtcta taagaggtgg catggatacc tcgagagtaa
    17941 ctgcaggtat atccagtttt ttccaggtat attttttata gccgttctct gtttctatct
    18001 ctggctcaag gtcgcccctc gttgtttgat aataaatatc cttattatcc ggtactttta
    18061 caattaaact gttttcttca atgggctctg tgtaaccata ctgctcggta taccagaaat
    18121 gaccttctat caaaggctct atttctttta ttaaatagct acagacaatt attgaaccag
    18181 gttctaatcc tggaaaatta atcactttat ctttgatatg tgaatatatc ctagcctcag
    18241 ttagctctgg tggagttaca tcatggactg cattctcgtc gacctcgata acttctccat
    18301 ctggctttat tgtatatgct ttaagtaatt caatctcttc ccttttttca tcatacctta
    18361 tcttgatttc actaaattct ccaatccctc tttcgttaaa tattttacaa attctattaa
    18421 cctgcgttct gtaaggaacc tcggtaaaat cgcataccgt ctcactcttt ataatcactc
    18481 ctcctgcatc agggtaatct tcctgggtag gagcgtttct tataatattt tctatttcag
    18541 tattatccac cgcagcaacc gcaacactta aaaacattga aacgaccacc aaaatcgata
    18601 tacttatgca tttctttccc attgccaatc tctcctctac ttcagtattt tgggcttttc
    18661 tctcaaataa ttgatagaaa gtcaaacaag aatgaacacc gcttgttttc taaggcaagg
    18721 ttacttacat caccacaacc actccagaaa aggcaaaatg atgtccagag tgcagtttag
    18781 cctattcttt aatttttgtc gagtaaagac taaaaaaccg tgctttgacc gggaaaaagg
    18841 caatagcttg tacatcacct gcaccatgtt tgtgggcact taacttccca gcaactgtag
    18901 agtagaagta gccagatgag tgacacaact ctctaataaa aaattcataa ttttttgtgt
    18961 agaagactac aaatattaaa aattagtttt tttatgataa aatcttgtca tgctgaaaaa
    19021 atactctttt tcttcagaat taataatttt aactacttta atttttattt ttactgtatc
    19081 tgtttttggt tacctgggat atcgttcttc tacccagcac ttcattatgc aggctttcga
    19141 caccatgcaa accattgttc gtctcaccca acaaagcatc tcccagacta tggaagaaag
    19201 aagaatcgaa ctcttgttac ttaaaaaaat gataggagaa aataagttag aaccctattt
    19261 gatagattac ctgcttcaat cttttgatac cttgttagta cttaaccgag agggaatagt
    19321 aataaaagcc tggcctgaga accaagaatc gttgattgga gaaaacctca gtcacagaga
    19381 atatttccag aaaacccagg aatccttaaa accttatcta agcggctata taaaatccat
    19441 attagacacc aatattatat cctatagcat tcccgtaata gaaaacggag aatttcaggg
    19501 agcaatagta ggaggaatgg ctttagaaca aggaaaaata aggcaattcg tatcttccac
    19561 tcaattcctc gaaactggaa gaattatcat cttagaccag aataataaca ttttactctc
    19621 cactaattca gaaaatatcg ggaaaacttt cccctctctt cctctaaaaa accagtctcc
    19681 tctgatacaa catattcaag gagaagatta tctaatgtgg atggaagaca tcccttactc
    19741 ttcctggaaa gtggtagcca gtatccagga gggagaaatt ttaagctatt ctcaaaaaac
    19801 tttaaaactt gctggaatct tggggatatt aattttagct gtcacccttt cccttcttta
    19861 tgtcctgctc aggaagctat cttggcctct ctctcatctt gctttcatag ctaaagaata
    19921 cgctcaagga aactattccc tctcccctct cgtagggggt cctcaggaaa tagttcagtt
    19981 atcagatgta ctgaatcata tgcggcaggc catagaaaaa agagaaacct tactcctcct
    20041 cgagcaaact tatcttaaaa ctttgctcca ggagatgagc caggcagtag tagttttaag
    20101 cctggaaggg aagctaaaat tcgccaactc ccagttgata aaactcactg gatattctga
    20161 agaagactta attggaaaaa atatcgacga gttatttcct tctggagaaa aagaaaaaat
    20221 accccatctc tacaaatggg atagcagcga agataaaaag gtggaaaggt cttacctctt
    20281 atcctcagaa ggcaagatga ttcctgtgct tctttccgcc agtcctttaa tcataaaaga
    20341 agagatgctg ggaactttga tggtaattac taacctcaga gaaatagagg aaaaagaaga
    20401 agagctaaaa caagctttgg aagagataaa aaccctcaac caggaattag aagaacggaa
    20461 tcaggaatta gaaatcgccc tggccaagct agacctgaag cttatggaag cagaccagga
    20521 aaaagaaact gctcaaaaaa tagctatcac agattctctc actcaacttt ttaaccgggg
    20581 atttttcgaa aagatgttcc ctcaagaatt cgaagctgct caatcaaata atcgcccgct
    20641 ttctttaatc gtagctgata ttgacctgtt caaaaagata aacgatacct atggccaccc
    20701 tgctggagat aaagtactcc aggacttggg aaaaatattg atatccagta ccagagaaga
    20761 agacctggta tttcgttttg gaggagaaga attcattatc atattacacg aagaaaaaga
    20821 aagagctcaa aaagtggcag aaagaatacg tcagagggta gaaaatttct cccgagaagg
    20881 caaagaagga ccaagcaaat tcaccattag tttaggagtt gccagctacc ccaaagatgc
    20941 ccaaacgcca agagaattgc taattaaagc cgatcaggct ctttatcagg ccaaaaagca
    21001 aggcagaaat caggtggtct cttaccagaa ttcctttact tctctttccc cttcccatat
    21061 cttttagcta acttcaagct aaatgaaaac gggaaaactc ctgaaaaagt tcctggtttt
    21121 tccagtatac tttcttatag aattgataaa attcttcgta aaatttttga gcttcaggat
    21181 cgggatttat aattgaatac gaagggaaaa aagagcctgg caattcattt agagaagaaa
    21241 cttctccccg gagcttacaa gataaaagaa atgctccctt ggccggagcc gaaggatagc
    21301 tactcagctc cacctttgtg cctaaaacat cacataatac tctctgccct ttatctgaag
    21361 aggcaaatcc tccgcttatc actattcggt gaggctctcc catcacttcc tgcaataaat
    21421 cataaataga gcgaacccga aatgccaccc cttcaaaagc agcttggaga agagctgctc
    21481 tttcatggaa ataggataac ccaaatataa tccctcgagc ataagcattc cagttaggac
    21541 ttcgctcccc agtcaaaaaa ggaagaaata agggacgttc ccgatgaata tcccattccc
    21601 actcttcttc ccctgttaaa tcttttaccc aggaatagac aattcctcca ttgtttatag
    21661 ctccacctcc aacccatatt ccatcagcca aataataaca ccagctccgt tctccagagt
    21721 caaatactgg atgaggagaa cacatcctca ctgctccaga agaacctact gttactacca
    21781 tttctccttc tcgaaaagct ccatcaccta aattagcaaa aggaccatct ccccctcccc
    21841 agataaccgg agtaccaggt agtaacccag tatcttgagt aaaatctccc ccttttaggg
    21901 gagcagtata ttctggagca acaagtagag gaagtttatc aagagaaatt cctgcaaaat
    21961 caagaatttc ctcgtcccaa gaaaaagagg aaaggtttaa aaggccagtg gctgaggcag
    22021 tagaataatc acagaaaagc tctccagtga ttttctctaa aatccaatct tttatgctga
    22081 gaactcgaga agttttttcc cacagatgag gctgattttt tctgaaccac cagactcgag
    22141 gaatatggta tatagtgtga agaggagcag cgtttttaaa atagagcttt cgctctcgat
    22201 aaagaggttt caatctctcc acttcatcag cacatctcgt atccatccag ttaagaagag
    22261 gatataaagg aattctctct ctatctacca gaagaaaact atggagcata gtatcaaaag
    22321 aaataaactc tacttctcgg ggagaaaaat cttccaacac ttcttctact gctcttttta
    22381 ttgcctggta gatttcttcc ggattttgtt cggcaaaacc aggagcagga gaataagatg
    22441 gatattctct tttactcgtt ttcaatattt ctcctcgagc atcaaatatg catactttac
    22501 aagaagaagt tccaatatct atagtaagca ctaagtgggc attcataact atactcccga
    22561 aaaagcgtta aaccctccat caataggcac tactactcca gtaacaaaag aagaggcagg
    22621 agaaagaagc cagattattg tcccgatcaa atcttctggc actccaaacc ggcctaaggg
    22681 agtgtgataa ataaccgcct ctcctcgagg ggtcaaacta ccatcttctt tagtgagaag
    22741 ataacgattc tggttggtga taaaaaatcc tggagccaga gcattcaccc gtactttagg
    22801 attgtattct tgagctaaat aaaccgctaa ccattgagta aagttactca ccgctgcttt
    22861 agccgcagaa taaccaacta ttcgagtaag agggcgaaaa gcattcatag aagaaaaatt
    22921 aattatattt ccttctcggc cactttctat catcatacga gcaaaaactt gagaagggat
    22981 gattgcccct ccaaagaggt tgagcatcac cactttttgc aaaggctcca agggcagatc
    23041 aaagaaagaa acctcaggag aggtagtagc atctttcata ttccctcccg cggcatttaa
    23101 tagagcatct attcccccaa attcctgttt taccttttgt gccacttcct ctacactttt
    23161 tttatccata gcatccatat aatacccttt ggcttctatc cccttacttt tcaaagcttc
    23221 taacctttct tccagaggag caatgtcggt gagaatgatt ttcgcttctg cttttcctaa
    23281 accttcacta atagctgaca tcaaaattcc ggctccgccg gtacacagca ccactttacc
    23341 ttgaatggaa aaaagctctt ctacataaga cattttattt acccctttct catctattct
    23401 actcgatatt ttcttccagg gaaaatagaa aacgaagatg tcgttccata ttttctctta
    23461 ctaaacgata atgttcttct tcatactgga aaaagagatg gctgagttct gacttaaaag
    23521 ctcgccagac cgaaccataa gaaatgtgta agaattgacg aattaggggg tgagaaaaaa
    23581 gaatgagaag gtcgctatct ggaatggtgt tcatgtctcg atgaatatct tctttggtaa
    23641 aagaaatttg gtaagctttg ctattttctt ctaactcttc ttcactcagt tcataaattt
    23701 ttcggaaaag ctgagggtct ttgtgagcta ctacctctaa acctttaagc cagctggtgc
    23761 ccgaggtttt caaatggaaa agttgattgg tctctcgaga aaaaacagga taaatgctga
    23821 atttgtcact tccagaatgc agactcaatc gataacctcc gatttcctgt tgcacaatca
    23881 catggctctg gagggagaaa gaaaattctt gaatattccc ctggtaatcg atagcttttt
    23941 caaaacttcc cgggaagcga ggagccaagc ttttaaaatc tatgcctctt cggtgtagct
    24001 cctcagctac aaaaaaatga acttcaggag aagttacccc ttctccttca tctagcgata
    24061 cctccaaatc ataagaagaa agtctctcct gcaagataaa attcattttc tctacaaaat
    24121 ttattaccgg aaaataaacc ttaaagagag gaaaaaactt ttcttttcta aaatctaact
    24181 ctaaacctcc ctgaagagtg agcgtccggt cagcataccg attaaaaatt tctctctctc
    24241 cttgaggcat actatcaaat atcttttgca attctttctc cgacttagaa agcatctcgt
    24301 agctggctac tccgcttcca tccagagtgt acatagaaaa accactctct atccccgccc
    24361 gaagatactc ttcatctttc aaatggtcag catccgcacc aaaaggggta agatttccgc
    24421 tctctaacac tccccaggcg gcaccgagca acacatcctg aaaggtcctt tccattttct
    24481 ggagttcccg gggagactgt tgagcaatca ccggaaaaac cggataatct ttaatcgctt
    24541 ctaagtgagc tcgagatact aaacccaacc tatctccaca acctaaggaa gttctcaccc
    24601 ctgcaggctg aggttttagg tcaggaaaat aaccactcaa ggttaaataa ttcttaaaat
    24661 gaaaaggata gaggaagaaa aatccccctt tttcttcctg tggttctaaa aaaagagggt
    24721 cggcaaagta aaatttctta tccctcgacc acactcctaa atatctctct ttttctccct
    24781 ttaccggata aattaccatt cccagttctt ctcgatgata attagaagaa taaactcgta
    24841 aaagaaggtt tatcctttcc cctattttct taactacttc ttttgtttta gaaaaatcac
    24901 tcattttttc ctcccgcttg ctttataaag catatttaga ttttggatct gaatatagtt
    24961 aaccgcaaaa tccattataa tgactatgag ggggaaagag tctccctcag tggggagcgc
    25021 ccccttataa cccccaaaaa agcaaaagaa aagaacaaag acttaaaaag caaataaaca
    25081 aaaactactg gtgggaaaat atccaccagt atgctggaga aaagacccca gcgtagcgac
    25141 cttgagtttt tgactttcga tataatttac tttttaaagt ttttaacatt ttaatcagtt
    25201 ttgcgggata ctataatgaa ccttcatctc ctgattaaaa ggtaagctga atatttcatc
    25261 ccataacctg gaaatatctt catagttttt cgttctcggg tctcttacca gaagttcctc
    25321 taatattttt ctatctcccg tagtaaaagc atctatagcc atttccatcc gcgttattct
    25381 tggcgtcaaa tagtatttta ttaccttatc tggcaaaggg ggctctattc tttcaggatg
    25441 tatcccgctt tcatcaactt caagaggtat ctcaacaact acatcgtcag atattccctt
    25501 tactaccccc tggtttagaa tattcaagac taacctggtg gctttattat tcactatcgc
    25561 gtttatgaac ggtacttgtt gctccccact catcttatcc atagaaaact cttctggcca
    25621 ggttttagtt aattttatcc cggagtcctt tgataattct attatcatcc tttttctctg
    25681 tctcagttct tcatagaatc tgggtctctc tatctcatta tctatacctc caaactttcc
    25741 atgccacttc ttcttagcat cgaggttata attatatttc caggttccat tgcgacaggt
    25801 atctccaact ggcaataaac cataaaatct atacatatcc actaccgcag ggcttagatg
    25861 aacatcccag gggttttctg gctgccaata ctgagatttt tcctctatcc atctatcaag
    25921 taaaggataa gcattttctc ctcggtagag aaaacgattt aaccatatac catgatttac
    25981 cccggcaact tgccagtcca cttcttccgg gttcaggccc agaacatgga aaactgcaaa
    26041 gagtcctgag aatccgtggc aaaaaccaat cgccttaaca gtagtttgtc tcaaaattaa
    26101 ctgagttatt tcaaaaacgg gattggccgt ttgtatgaag taagcatcag gacagatttt
    26161 ttctaccgat tttgctatat ctaaggaaag ctttaaatga ttataattgg tgaatgtata
    26221 ataatctgac accatattaa attcctggct gtctattcct ctataatacc catatttctc
    26281 cgctacatcc ctcatgatct catagctcac ataaccatct tcatggcctg gtgctcggta
    26341 caatgctgta ttaatcacaa aatccgcccc ctgcagtgat tcttccctat ccgttgtttt
    26401 ttctaatttt aagtttcctc ccacctcatc cgtatatctt tttgccagat tatatactgc
    26461 ttctaacctt tcttcatcca catccattaa cgaaatcaga cttcctgaaa gttctttcgt
    26521 ctttgctaaa tctccaacta atgcaagcgt ataccttata cttcctgctc ctataaaaga
    26581 tatctttacc aatttcatac ctcctctacc agtaatttaa ataagtagaa aataaagagc
    26641 caatcttcag ccagggaaaa tactgtacct atttgttgca attcttgaca taattcttat
    26701 caagcatcaa ataacagtat tttattaccc aacttctttt ttctgtaaac attttcctct
    26761 acacccttac tccgcaagag gacctgatta ctaattccgg cttcaagctt atctgattat
    26821 tcttaacttt ttccccgttt atcaatcgaa aaagtaaatc agcagctaat tttcccatca
    26881 tatatttagg ctgattgaca gtagtcagtg gtggatcaag ataagcagaa agaaaaatgt
    26941 catcgtatcc aatcagggaa atatctttag aaatcaacaa attcttctcc cttattgctt
    27001 tcatagctcc cagagctaca agatcgttat aagcaaaaat agcactagga ggattatcaa
    27061 tgctcaataa tttaagagta gctacatatc ctccttctac cacaggcaaa ttaaaactca
    27121 aaaggccttt cttaacctct cttcccgatt cctttaaagc tctcagaaat cccttttctc
    27181 gccacagact gggttgagca ctatttggac cattgattag agcaatcctc tcatgcccca
    27241 aactcaaaag atattttgtt gcctgataag caccgctttc ataatcagca aaaacactgg
    27301 ggatgccctc gattattcta tttataatta cacaggggat atcactattt actacttctc
    27361 taatctgctc ctctccaatc cgggaggacc ctaagataat gccatctact tcgcttacct
    27421 ttagagtctc aacatattgc ttttcccgtt ccatatcttc cctggtattc cataaactca
    27481 cgctataacc agaatcaaaa gctaaatcct ccacaccatg aacaatttct ggaaaaaaag
    27541 gattggagat atcggtgacc atgagagcta tgattctact ccttttcctg gtggaagatc
    27601 tttttctact tcttaccgga gtataattaa gttcttttgc tacttgcata acttttcttt
    27661 ttgtttcctc gcttattaca gggtcatcat taagcgccct ggaaacagta gaaggagaaa
    27721 caccacttct ccgggctaat tcatttatag ttatcttcac tatcctgcct ccattttcta
    27781 cgcttaccat agcacagttt ttgaaatttg caagatatta tgaaaaattt ctaaaaaata
    27841 tttttatgca gataattgct agatgttttt cataagttat gcaaattctc taaagaaggc
    27901 tttactgcta cggaaagaag gagggcttta ctgaattctt ctattcggct accctgggga
    27961 aaccctacat cgcaaaagtt gtatttttac attaaagaat tctcggcaag aataaaccgg
    28021 gataccgctt tgccttctcc ggagtggtga tgcaaatacc tcacccggga aagcaagcgg
    28081 tgtccacctt tattttctac caattatctg gagggaaaac cagtattaag gaaaattccc
    28141 tcgtatcttt cttgctttaa ttgtccgatc aagggccaac atataagcag cttctcgcag
    28201 agggactcta ttctgctctt gaaattccca tacttcctgg aaactcctct ccataatttt
    28261 ctggagaaaa gtattaactt tttcctcatc ccacattaaa gattgaatat tttgaaccca
    28321 ctctaagtaa gatactacca ctcccccagc attagataga atgtcaggaa caacggttat
    28381 gcccctttcg ctgagaattt gatctgcctc aagagtagtg ggtccattag ctgcttcgac
    28441 gataattttt gcctttatat cccgggcgtt atctttagtt atctggtttt ccctagctga
    28501 aggaactaaa atgtcaacct ctaaagttag taactcctga ttagtaagag atgaaatccc
    28561 ttcagcttcg tacccctcaa tgagatgaga aggatttttc tttacatagt ttgatatatc
    28621 cattatgtca agcccttctt ttttatacat tcctccgctg atatcactta tagctacaac
    28681 tcgatatcct tcctgatata gaagccgggc tatcgcttcg cctacttttc cgaatccctg
    28741 aatagcaaaa gatgctttac ttaaatcaat tcctttttgg agagcaattt gattgatggt
    28801 aaataacacc cctctaccag ttgcatcttc tctccctagc gatccaccaa tttctatagg
    28861 ttttcccgta actactcctg gaacagtaaa acctttaaat acactatagg tgtccataat
    28921 ccatcccata atctgggcat tggtgttaac atccggagca ggaatgtctt tttctggccc
    28981 gattaaagga agaatcattg ctgtataacg gcgggtaacc ctcataagct cccgggaaga
    29041 aagtttcccc gggtcacatt gcactccccc tttagctcca ccataaggga tattaaccac
    29101 ggcacatttc cacgtcatta gagcagctag agtcctcact tcatcaaggt cgacatctga
    29161 agaataacgc aatcctcctt tacacggacc tcgaacacta gaatgctgga cccgataacc
    29221 agtaaaaacc ttaacatgtc catcatccat ttccactgga atagaaacag ttagttctct
    29281 ttctggataa cggagaggaa cataatcatc ccagcaaact cccagctttt ctgcagaagc
    29341 atctaacacc ttgagcatgt tcgcataaga gtgaccttgc tctgacaagg aagaatcaca
    29401 acacttttgc tcattttgaa tttcaatatt atggttacag ttatctaagc tcaactaatt
    29461 tacctccctc gttttgctct aactatcttt cggataaaca acatcagcca gaaccagccc
    29521 tttaaactct tctactaaac tttacctgac tttttgtttc aaattaatct gctttccggt
    29581 aacctttctc tgttgataac aaatttagaa aggctccatt ttcctcactc cagagagaaa
    29641 tcaaagctcg ggatatcttc ttctattttt tctgaactct tttcctcacc acaacctgag
    29701 tcgaaccatc aaccagaagc taaattctca actccgtaga tgattttcgg gaaagaggaa
    29761 ctggagatat cggcgaccac aagaactatg attctacttt tctttttgtt ttttcactta
    29821 ttacagggtc accatcaagt gcttaaaagc agcaggagga gaaacaccgc ttcttaagat
    29881 taattcattt atgtttatct tcactatcct gcccccattt tctgcgttta taatagcaac
    29941 aatttttgaa attttcaaga tatcgcagcc tcatttaaat atatgctgaa tttgtattaa
    30001 aattcgattc tctcacctgt ctttttatca aaaatgtgta acctgctaaa atcaacactc
    30061 aggttaactt ctttaccaat atctttttca gttaattccg gtaatttggc gagcattatt
    30121 atgcttccca ttttaaagta aaccaaaaca gcatctcccg gttatgtcct atatcctcgt
    30181 aaaaatagaa aagtggatgg ccattgtggt aaagtacatt ttatggaaat tct
//
LOCUS       DAOSVE010000011        30148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_1033_length_30148_cov_70.979198, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000011 DAOSVE010000000
VERSION     DAOSVE010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 30148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30148
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1033_length_30148_cov_70.979198"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            585..1172
                     /locus_tag="PK016_01835"
     CDS             585..1172
                     /locus_tag="PK016_01835"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXL01851.1"
                     /translation="MIYYISKYLAIFLLKIFFSFQVKGASRIPLQGPCILVANHSSNL
                     DPIVLGCASPRRVYFVAKEELFRNPLASFFLRHLGAFPLHRGEGDKEAVKRIFSLLKE
                     GKVVGLFPEGTRNQGELGDFQRGALKLLIRAGVPVVVAGIRGTYESLPRGKNIPHPFP
                     IRVSFSPPFEVKNWSVEEVEKKIREEMGVLLGEKD"
     gene            1159..2526
                     /locus_tag="PK016_01840"
     CDS             1159..2526
                     /locus_tag="PK016_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HXL01852.1"
                     /translation="MRKINKEALLVFIFLLTFFSVAEGAPWEVERIIEEVGGDLPFLD
                     YPVGILVDDQNRSILINDWGNNRFLSLSEDGQIKKVLDSLKSPAGLGKDEERGRIYIV
                     EQKANQVRIIKSDDFSTLGFLKAKTPLQEPRGIWVDEEGKIYVADTGKSRIVVFSPGG
                     EELFTFGKEGMGNDEFYYPRGVAVDEEGHIWVVDTQHHCIKVFDEKGNFLFRFGQGGE
                     GKEDFNQPRYLFIQEDWVFISDYRNHRIKVYNRGGVLQEIIGKEGTGEGEFSFPEGLW
                     IDEGGRLWVADAGNNRLQVIDVNFLINPKLYLASLLEERKDQEFMQVMERLSPEDKKD
                     PEVARLLLEFFRKRGNLDQAIYQAEDLFLNDSENRDYWKKTLGELYYEKGVHLRGEGK
                     LKEAREFFHKSWQNGNSHSLFLYLWTSFLLLGGSNLFILILAIVLLFLIMIFSRLRKE
                     RYRGW"
     gene            2529..3380
                     /gene="amrB"
                     /locus_tag="PK016_01845"
     CDS             2529..3380
                     /gene="amrB"
                     /locus_tag="PK016_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HXL01853.1"
                     /translation="MVRKAAVAGYFYPGRERELRQMIEELFYSEWGPGGKEEELKKWK
                     EVSGVVSPHAGYIYSGAVACWSFYSLSLASPVDTIVIIGPNHRGVGKKVALSDTDVWE
                     TPLGEVAVDLEAVEFLASHYPLFSPDSRAHQLEHSIEVQIPFLQYFLPYPFQILPVTV
                     WDQTLEVALEISQALQDLSLKRKIVIVASSDFSHYEPAQVAEAKDSHAISYIVSLQTE
                     EFYKVIRRENISICGPAGIAALMDFHKSCSRGEGKLLFYSHSGKITGDYQEVVGYTSL
                     IFPVATD"
     gene            3507..4970
                     /gene="aglA"
                     /locus_tag="PK016_01850"
     CDS             3507..4970
                     /gene="aglA"
                     /locus_tag="PK016_01850"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032450 - maltose alpha-glucosidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase AglA"
                     /protein_id="HXL01854.1"
                     /translation="MAATKLGIIGAGSAAFSLRLVGDICKTKELSGSLISFMDIDKDR
                     LNAVYILARKYAEELGANLKFETTTSVEDAIKDSSFVVNTALVGGHGYFEKVRQISEK
                     YGYYRGIDSQEFNMVSDYYTISNFNQLKFMYDVAKMIEKFSPKAWLLQAANPVFELTN
                     LISRNVPINMVGICHGHHGIDQIIEVLGLDKNKVDWQVAGVNHGIWLTRFLYEGEDAY
                     PLIDKWFEKGLVSWKPTNPFDDQLSPVAKDMYEFYGKMPVGDTIRNGSWKYHYNLETK
                     KKWFGEPWGGVDSELGWKWYQERQAEVAIIMQKVAKYFQENPEARLLSQKTYDEIISL
                     AKGEVKEGFAQEVFRILDPERKSGEQHILLANALLNNEKVDLVLNVLNKGTIPGIPDD
                     VAVEIPVYADKDGIHPYQIEPDIPERIKKMYLMPRILRMEWALEAFLTGDRRVLEEFL
                     IRDPRTRSYDQVVKVIDEILALPENEEMKKHYSNSRK"
     gene            5945..6946
                     /locus_tag="PK016_01855"
     CDS             5945..6946
                     /locus_tag="PK016_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HXL01855.1"
                     /translation="MAKLKEIAERANVSVATVSHVINGTRPVSDALRKRVLEAMKELG
                     VEVSVSHPKNESSHLIAMVIDDIFNPFFIEVFAAAESTARTMGYHLLLLSIEKDYDTF
                     YLQDLGDKKVDGFILATRLSLSYLKEFFSAQIPLVFLGGVLDSPFSRSILFPDEEGAY
                     LATRHLVDLEHSRILCVGGPSKSSLFRNRFQGYKRALREKQIPVDPQLMVEITPTFEE
                     ARQLGLKYLSLENQISAVFAHNDPTACGIMAAAQELGRKVPEDLSIVGFDNTFFTQLT
                     CPPLTSVEIPKKLIGKRSVELLIRLMNNDGVQSYVIEEAKLIERKSTAFFRKNNLGV"
     gene            6950..8332
                     /locus_tag="PK016_01860"
     CDS             6950..8332
                     /locus_tag="PK016_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01856.1"
                     /translation="MDKEAKITVVGAGSAAFGLSTLVGILRHPDLQGVELFLHDINEN
                     GLEKIRKLAEKMNNAWGSGMKINSSVEREKALEGADFVVLSIAIDREKCWRLDRDIAI
                     RYGINHYAENGGPGAFAHTARNLSAIMPILQDMEKYCPQAWLLNFTNPVPKICTAANL
                     YSNIKTIGICHQINFGYFILAALFADELDLHLPRDFNFRWNDQSLSLFQIMSKKAKEK
                     FLIRAWGLNHFTWMVNVTEKGTGRNVYPEIEKRMETLPPSFEPLTQEVFQIFGLLPVP
                     GDCHLCEYLPYTHNINRKTWERYDIQMYDFEWAEKGRQKMWEKIDRVVEGKESLKGWE
                     EVETERGEFIIAGILKNLNSYEEAINIPNRGYIVNLPDEVIIEVPAILGSEGPVGLGG
                     CELPEPIAELCRRQSLINELVVRAIVEEDRKLAQEAFALDPMVDDLEVAKKLLDDYLK
                     TFDSYLSFKL"
     gene            8452..10332
                     /locus_tag="PK016_01865"
     CDS             8452..10332
                     /locus_tag="PK016_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL01857.1"
                     /translation="MRKIVWSVVLAILLVGAAGVSFAQVLPEIPRTETLIVDILHGRI
                     GNPGNFNVWIPGSQAGHGLQQMLMDALWYVDPQTGEWINALAAEEPKYNEDFTKMTIK
                     IREGIYWSDGEPFTADDVVFTVKNIQSHPGMNSQPQLQEYVKDIYKTDDYTVEVELTK
                     SYSRMHNIFTSLVYGALFMQPKHIWEKVENPLAFDFNPPASLGPYVLDSYDPQGYWFL
                     FKKRDDWQRTSVGQLYGEPKPNYVLFIYYGADDRKVMAQARHELDMIFDLTPEAWEVL
                     RERNEYSVVWYKDFPWAWMDDVNSRGMFLNNARYPFEIKDVRWALTLAVNVEEIVISG
                     YAGIERVSSLHSCPTQYFTEIYFKPLEPWLEDFTIDGDYKPYDTDVAYRIAEWAEKQY
                     NIKVEDPEAVWGIGWWKYDPQKAEDLLLKNGFSKDQNGKWLLPDGTPWKISIIAPADF
                     EIDATRLAFAVADQWRKFGIEVNVETLESGPFFSRQAIGDYDVGSFWGGMLASSGIPD
                     IWTFINGFKSEFLQPLGTAVTTANNYRWKVPERVDQIIDEMGTLYPGDPKIIELAQEF
                     YKLAVEDMPTIPMVINKKFNANDNYYWTNYPSADNPYMANVCWWAEFKFMLPRIEPTG
                     RE"
     gene            10535..11545
                     /locus_tag="PK016_01870"
     CDS             10535..11545
                     /locus_tag="PK016_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL01858.1"
                     /translation="MNALKTYVLPRLIQYLVVIFVGITITFIIPRLTPIDPVQSAINR
                     LTSYGGIYMEPQAVESLQNTLKELYGLEGSLLEQYFAFWRHLFTLDFGPSLSMFPAPV
                     IEIIANSLSWTVFLLLTTLLISWIIGNILGGLAGYFKDKKWSNVLAIIAMSIYPIPYY
                     VMALILVILFVYVFPVFPMMGGYSIGVQPSLTWAFILDVLRHSILPAISLIIIGIGWW
                     FLSMRSMTSNVVAEDYVTYIEFAGLPKHKILFSYVMRNAILPQVTALALQIGGIFNGA
                     VITEVAFSYPGVGLLLKNAITNGDFNLMMGIAVFSIIAIATASLVIDLVYPLFDPRIR
                     YR"
     gene            11555..12418
                     /locus_tag="PK016_01875"
     CDS             11555..12418
                     /locus_tag="PK016_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012896047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL01859.1"
                     /translation="MFVILKDLFKKDRKFLFGFTVICILAFLAILSFFSPYDPRSWSV
                     VPKDRPPSFQHILGTNSMGQDIFWNTTHALKNSFTLGITTAVVATIIGTIVGLIAGYR
                     GGTLDRFLMSINDSFIVLPSLPILVFLSFSLREKMTMFVLGLTLSVFSWPWGGKQVRA
                     QVLSLREREFTYTSVFSGMNMWKVVFREHLPFVIPWVIANFINTILWALGMEITLSVL
                     GLTSLETPTIGTTIHWAMQYQAIFRGIWWWLLTPIILCVLLFIALYMLSVSISEFLDP
                     RTRLQRIRMGG"
     gene            12425..13450
                     /locus_tag="PK016_01880"
     CDS             12425..13450
                     /locus_tag="PK016_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL01860.1"
                     /translation="MSLLEVENLSAYYITHAYGIKRDVRAVDNITFSIEENEIVGLAG
                     ESGCGKSTLIKVLLAIIKPPLRILSGQVNYSYDDKKFNLLTLEGKQLRRVQWEDISYI
                     PQGSMSTLNPVRKIRKTFEDVIKAHKRGESKEEYEKLMEEHIKYLGLPLEVLDSYPHQ
                     LSGGMRQRTTIALATILRPKIIFADEPTTALDVVVQRGVIQMLKKLHKEQKNTMLIVT
                     HDMAVHANVAEKIAVMYAGKIVEIAEKKELFTDPKHPYSQFLIQSLPRIGDKKRRTSA
                     PGNPPSLANPPSGCRFHPRCPYAKDVCKNYAPKLIKYEEFHNIACFLYSSETEEGEKI
                     VQEVIRS"
     gene            13425..14423
                     /locus_tag="PK016_01885"
     CDS             13425..14423
                     /locus_tag="PK016_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL01861.1"
                     /translation="MSRKLLEVKNVVKIFHIGGRFLGTKLKAVNNVSFEIDSEKPEIL
                     TFAGETGSGKTTLARMILGLEPPTLGEITYKGKNVTNIKKREELLEYMKEVQPIFQNP
                     FETFNPLRKVDSYLFETAGNYNLAKTRKEKMKVVEEALKSVGLTLNEVQDRYPNELSG
                     GQLQRTSVARALITRPSLLIADEPVSMVDASLRMSIVNLFSDLKNDYNVTVLYITHDL
                     ATAYYISDRIAIMLRGDVVEFGDIDKVMLSPLHPYTQVLLKSVPQPDPEGTWKEDIKL
                     SSLEVKEFSRLGCKFYERCPSAMKICKDVEPPNVFKNGRDVKCHLYSQKLEEREEA"
     gene            14489..15289
                     /locus_tag="PK016_01890"
     CDS             14489..15289
                     /locus_tag="PK016_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXL01862.1"
                     /translation="MQYIELYEARVPVIGLGTWDLRGEICQRAVLSALNLGYRHIDTA
                     QSYANEEEIGKALQSSPVPREEIFLTTKVWYTHLRYSEVIQALEESLRKLKTDYVDLY
                     LIHWPSEDVPLEETMSAMIKVKEEGKTRYIGVSNFDVPLLEKARQISPYPILTDQVEF
                     HPYYSQRELLNYCQKNKVILTAYSPLARRRLSDDLLLQEIGKKYGKTANQVALRWLIQ
                     QEMVVAIPKAASPEHQRENLDIFDFELTPEEMEKISRLDRREKVASRI"
     gene            complement(15337..15822)
                     /locus_tag="PK016_01895"
     CDS             complement(15337..15822)
                     /locus_tag="PK016_01895"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HXL01863.1"
                     /translation="MKRIGQVVKTEGKYSWVKMERGTSCGEGKCPLSSPLIDDSKSEF
                     YMVKAYNELSASPGDRVLVEIKDHAALGIAFLIYIFPILLTLFVYFLLRYLTPLTLYH
                     YLGVAISIIFSFFLLKKEDQRIQINYRITDLANSTCESCPLSDNTTRFSYPPGKEAIT
                     R"
     gene            complement(15825..16133)
                     /locus_tag="PK016_01900"
     CDS             complement(15825..16133)
                     /locus_tag="PK016_01900"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HXL01864.1"
                     /translation="MAEFKKVAEILELSSGRKEITIEENKILLLNSKGKIYAIGSRCP
                     HKGLSLAQGKIEENIITCPYHGSHFDITTGQVVKGPAKENLPVYEVKVEGKDILIGLQ
                     "
     gene            16437..16823
                     /locus_tag="PK016_01905"
     CDS             16437..16823
                     /locus_tag="PK016_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01865.1"
                     /translation="MVTISKGRVFRFYIESRYPFGEFALIRSRGNLGEVGFVLADENV
                     ERWPSLYRKAWEIANYFRLRIKELGLDNIAVAGVGRDLDFITASIIIDLSAFEKEDIE
                     KVADQIMGILKEANPYYNNKTGEIDE"
     gene            16816..18018
                     /locus_tag="PK016_01910"
     CDS             16816..18018
                     /locus_tag="PK016_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXL01866.1"
                     /translation="MSEKVGFITMGESKGIVENLPELRVGMLGYAFMGKAHTNAYKKY
                     PYIFWPPVAIPRLIAICGRNEKAVREAMIRYGYEKYYTDWREMVEDPAVQILDNSSPN
                     YLHAEPSILALEKDKNVLCEKPLAGNAQEAKKMWEAAQKSSAKAMVCFNYRFVPALRL
                     AKEFISSGKLGEIYHFRAQYLQEWIMDPDFPTIWRLDKSAAGSGALGDLGAHIIDLAR
                     FLVGEITTVSALTRTFIKERKVAGDPGKKVPVTVDDAFVALVEFERGAIGTLEASRFC
                     AGRKNYQFIEINGSRGSIRFNLERLNELEVYLVGEEPKIFQGFHDVLVTESFHPFYEY
                     WWPQGHIIGWEHTFIHAVAHFLDAIVNNKDVAPYGATFEDGYRCAVICDTILQSAESG
                     KREAIPQD"
     gene            18046..18303
                     /gene="purS"
                     /locus_tag="PK016_01915"
     CDS             18046..18303
                     /gene="purS"
                     /locus_tag="PK016_01915"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HXL01867.1"
                     /translation="MKYRGKVIVTLKPGVFDPQGVTIRSALHTLSYREVEEVKTGKYF
                     EIVLELENLSQAEKRLQGMCDQLLSNPVIESYSYQIEEMKD"
     gene            18312..19010
                     /gene="purQ"
                     /locus_tag="PK016_01920"
     CDS             18312..19010
                     /gene="purQ"
                     /locus_tag="PK016_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HXL01868.1"
                     /translation="MKFGIVVFPGSNCDYDCYHVLSQVLGVETVFIWYGDRNLKDCDC
                     IVLPGGFSYGDYLRAGAIARFSPVMEKVKEFAENGGLVIGICNGFQVLTEAGILPGAL
                     LPNRDGRFICRHVYLRVENNQTPFTLLFTPGEVVKIPIAHQQGNFFIDPLCQEEAKKQ
                     VVFRYCSSEGEVGSRYNPNGSWENAAGIISREGNVLGMMPHPERAAEAILGSADGRRV
                     FLSVIKWWEEKKWK"
     gene            19025..21257
                     /gene="purL"
                     /locus_tag="PK016_01925"
                     /pseudo
     CDS             19025..21257
                     /gene="purL"
                     /locus_tag="PK016_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
     gene            21212..22615
                     /gene="purF"
                     /locus_tag="PK016_01930"
     CDS             21212..22615
                     /gene="purF"
                     /locus_tag="PK016_01930"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HXL01869.1"
                     /translation="MDGKEKCGIFGIYGLKNASELTYLGLFSLQHRGQESAGIAVSDG
                     KTVREKKGMGLVSEVFTPQSLKGLPGHIALGHVRYSTTGSSSSRNIQPFIGESRFGHL
                     AIAHNGNLTNALALWKKLSERGAVFQSSMDTELILHLIAQGSFDGLEDSLKDALWQIQ
                     GAFSLGILSSHQLIAVRDPWGFRPLSLGKLGDGYIISSESCAFNVLGAEFVREIEPGE
                     MIIIDEKGLRSFFYAYSPRKAFCIFELIYFARPDSVVFSNQVYQARKNMGKVLAEEED
                     CSADMVVPIPDSGTFAALGFSEASGIPLEFAMVRNPYVGRTFIRPEQGARDLAVKIKL
                     DPIRELIRGKRIIVIEDSIVRGSTSRQRIATLKEAGAREVHMRVSSPPHIFPCHYGID
                     FPTRRELIASDKSIEEVKKYLGLDSLRYITIEGLKNSVTPPGENYCFACFNGDYPVTP
                     QEGGKLILEKRQVINQC"
     gene            complement(22660..23139)
                     /locus_tag="PK016_01935"
     CDS             complement(22660..23139)
                     /locus_tag="PK016_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HXL01870.1"
                     /translation="MSLALVLYYTRTGITEKMAQTIAGTLSQEGVSTDLVDVSQFQVD
                     DFKKYDLIVIGSPTYYGTMAAEIKEMLDESIKLHGDLNGKIGGAFSSSANRAGGNETT
                     IWSILSALLIHGMVIKGVAEGSHYGPVAVSTFDDGAKKDCQLYARELARLLKSLKPA"
     gene            complement(23156..25531)
                     /locus_tag="PK016_01940"
     CDS             complement(23156..25531)
                     /locus_tag="PK016_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HXL01871.1"
                     /translation="MKISLNGKWRVYDEEKEFDFEGDVPGTVQGDLVRLNLMPHPYVE
                     TNEKLFRRLEEKSWVYERTFYIDKLDQETNYDLVLEGVDTLSQVYVNGEFAGKTEDMF
                     IEYRLPVKEYLKEGENFLKIKIESPLEIPKILEKNYGKLHAGEETARVYIRKAQYSYG
                     WDWGARIATSGVYRNVYLDCYPDARIYGSTAYLEDLDGTVVFSGYIDSIGNEVETYKV
                     EIILDNKSIDSFPVTRNVKNFKFEGKRKIKDIKLWYPNGSGESYLYPAEFKLFKNEKE
                     IYTEKKKIGFRTVKVIRENDGEGESFIFEINGKKIFAKGANWIPGENILSWLTKEDYK
                     TLLQMAKDAHMNMLRIWGGGLYEDPAFYDYCDELGILVWQDFMFSCAEYPDHINWFRK
                     LANEEVRHNVIKLRSHPSIAIWCGNNENNWGFEEWDYKIKVDGKNLGNRLYLEDFPLI
                     CSEEDPSRLYWPSSPYGGERANSNKAGDRHVWEIWSGWQDYKYYYRDTSKFVSEFGFQ
                     AAPDAKTIDFFASEKDKNLFSPVMLNHNKQVEGPERLLKFINNHYGLISSFDSLVYLT
                     QLNQAEAIKAGVEHWRSRKYRTAGTLYWQINDSWPVFSWSSIDYFKRPKALYFYTRRF
                     YSSLLPIAKNKDGQIFISIINDGGYSEVNLEFELWGLKGNLLKKETYRNFKVPEDSVI
                     LVGQLEVDPSILESAVAYLKLKQKEEIIIENHEIFADLRLHHLEDPRISYEKAGKDLV
                     LKCEKPALGVNIRVNDENYSGENFFALFPGKEKIIKDIEGEISIKSMFDYL"
     gene            25776..26099
                     /locus_tag="PK016_01945"
     CDS             25776..26099
                     /locus_tag="PK016_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01872.1"
                     /translation="MIEEAIQEIRELENLKEREISSAELEAAKIMAQVEDQIAEMRKK
                     ALQEVEERAQSLRAEILKEGEEEAKKIEEEFRHQAQKLESAASSRMKDIVSQLVKEMG
                     EEYGN"
     gene            26089..28056
                     /locus_tag="PK016_01950"
     CDS             26089..28056
                     /locus_tag="PK016_01950"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HXL01873.1"
                     /translation="MAISEMEKVGIIAHHSLRKGILDRLQDLGVLEISEDEKELFDEF
                     VAPSGELGELERRLGELGYCLDFIDKYRSEKPGIVQSLFPAPIHMEKEDFLSCSFDYI
                     PLYKTFLGWDEELNNIRSNLNRLNSNLEFMKRIQSVKVSLENLRGTLYTRSFLLEVPP
                     ERKNVLEEKLKEKDLIWLGEEFPGKGKNTWWWVVIHKSGEEAMREILAELSLSPVSLP
                     QPFEGTPQEVVEDLEHRITELQEEQSKLLEKAALQEKFEKELKILYDYYLSLWERHQS
                     ETRLLKTKETCYISGWVKKEDLPLIEESLSQVGKEWVLYHREPEEGEDVPVILENNAW
                     VNNFAVLTHLYGLPNYTEIDPTPFVAGFFFFFFGMCLGDVIYGLVLALFGFIAAAYLD
                     VSDSTKTFMRMLAWGGVGAILVGICTGSWLGDLFDYLPSSLSFLTTLKNSLMVIDPLN
                     NPLPMLIFSLGIGIFQILIGIMLSTIKEWKRGHRAVAVMDHLSWFVFLTSIVLYIVGI
                     AALPLVASVALPVVIGSAIFLVATQGRHQKNPIMKFLSGLMSLYGLVSYLGDTLSYSR
                     LFALGLSSTIIAILARTLGSLFGGYPYIGWLIALLVALAFHVFNLLMSGLGAFVHSAR
                     LQYVEFFNKFYEDGGRNFKPFGYKTKYVKIS"
     gene            28074..28547
                     /locus_tag="PK016_01955"
     CDS             28074..28547
                     /locus_tag="PK016_01955"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HXL01874.1"
                     /translation="MIDGLAFALLGSALAIGLAGIGSALGVGIAGEAGAGVLTEDPNK
                     FGQVLLMQALPGTQGIYGLLAGFWVLIKLGLFGGTPVEITVAQGLQILFACLPIAIVG
                     LLSGISQGKTSAASIGMIARRPEETGRAIILPAMVETYAVLSLLATILLLNSIQV"
     gene            28560..29144
                     /locus_tag="PK016_01960"
     CDS             28560..29144
                     /locus_tag="PK016_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HXL01875.1"
                     /translation="MPLEDILKKIEKEAELKKKRILEEARAKANAQLERAQREIEKEA
                     EQLLSNHKREIELEVQRKISEARLAGKNEVGKVKYEIWERLRQELIEAFIKNVEAQEE
                     EWCKNFLSEQIINGDEEVWMAPREVEILGSNFLEKLNQEKGLSLRFGGVAEDLERGFL
                     LKRGGMVVDLSFISAVEDYIRKNEGRISALLFEG"
     gene            29148..>30148
                     /locus_tag="PK016_01965"
     CDS             29148..>30148
                     /locus_tag="PK016_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HXL01876.1"
                     /translation="MSVDLRKPASSSGDTRYTYLVGRVRELERGLLSQRTLETLLRSE
                     NLEQALRGVAEIPYWGEAFQNVPSRTYDIDRVLVTHYWQNIQEINSHSPGNKIARFFI
                     WPFDFSEMKLILKHRLVGKALEDTYPTTLDVVRVNRFLGGETREYLSDLWIKAITDSL
                     EAYENYRVIQVVEIVLDRHYLEQVYLLAQEMESKVLKNWLLAFVLLAYFRAVFRARYQ
                     QRKGELVRLLFFAQPLFPEGKLVEMLNASPEKVAEEVGNFGFDFLLPEKGVFRDDPSY
                     LAEIEKNMDNYLLDFIRSYRWEAFGPEPVFGFLWAQGIDVKNLRLILEGKYFGLENE"
BASE COUNT         8862 a         4983 c         6815 g         9488 t
ORIGIN      
        1 tttttgcttc ttccttttgt tttttgcttt tgggggttat aagggggttt accccactga
       61 gggatgtttt ctccctcagt agtctttgtt tttttaagtt tcttatcttt ttctttacct
      121 ttttggggga ttataaaggg gtctcccctt tatagttcgg ggttataagg gggagaagcc
      181 cccattattg ctgtttacag aatagaaaac tgaatggctt ttgcgataga acaaatcttt
      241 ttaaaaattt tgtagaatct gacttcttat tattttcttc ctatatctgc agactaaata
      301 caacttgttc ttctgcctaa tctgcggagt ctatttataa attacagaac taaaagcccg
      361 gtatacgaac aattaagata gctcggattt attgcctgtg gggattttat ttgttattct
      421 ttgttagtac ctgtttcatt ttgatgaggt aaaacgaaaa agtcatggat caaagctttg
      481 atttatttga gaaaattcag ttctaatata gaattttaac atcctgactt ttctctaaaa
      541 ttggagtaga acctcatggg gcaaagggag taaaatagag aaaaatgata tattatataa
      601 gtaagtactt agccattttt ttgttaaaga tttttttttc ttttcaagtg aaaggggcaa
      661 gtcgtattcc attgcaagga ccttgtattc tggttgctaa ccattccagt aatcttgacc
      721 ccattgtgct gggatgtgcc tctcctcgga gagtgtattt tgtagctaag gaggagttgt
      781 ttcgcaaccc cttagcttct tttttcttac ggcatttagg agcttttcct ctccatcgag
      841 gagagggaga taaagaagca gtgaaaagga ttttttctct tcttaaagaa ggaaaggtag
      901 taggtctttt tccggaaggg actcgtaatc agggggaatt aggagatttc cagagaggag
      961 cgctaaaact tcttattcgc gctggtgtgc cagtggtggt agcgggcatt cgggggactt
     1021 atgaaagtct accgcggggt aaaaatatac ctcatccttt tcccatccgg gttagttttt
     1081 cccctccttt tgaggtcaaa aactggagtg tggaagaagt ggagaaaaag atcagggagg
     1141 aaatgggggt attacttggt gagaaagatt aataaagaag ctcttttagt gtttatattt
     1201 ctgttaactt ttttttctgt ggcagaggga gctccctggg aggtagaaag aataattgaa
     1261 gaagtgggag gggatttacc ttttttagat tatccagtgg gtattttggt agacgaccaa
     1321 aatcggtcaa tcttaattaa tgattggggg aacaatagat tcctttcttt aagtgaagat
     1381 ggacaaataa aaaaagtttt agatagcttg aaaagtccag cagggttggg aaaagatgaa
     1441 gaacgtggaa gaatctatat cgtagaacaa aaagccaatc aggtgagaat tataaaaagt
     1501 gatgattttt ctaccttggg ttttttaaaa gctaaaactc ctttgcaaga accccggggg
     1561 atctgggtag atgaagaggg gaagatatat gtagctgata ccggtaaatc tcgaatagtg
     1621 gtttttagcc cgggaggaga ggagcttttc acctttggta aggaaggcat ggggaatgat
     1681 gaattttatt atcctcgagg ggtagcagtg gatgaagagg gccatatctg ggtggtagat
     1741 actcaacatc attgtataaa agtatttgat gaaaaaggta attttctctt ccgttttggc
     1801 cagggaggag agggaaagga agactttaat caacctcgct atttatttat tcaagaagat
     1861 tgggttttta tttccgatta tcgcaatcat cgcataaaag tttataaccg aggaggggtt
     1921 ctccaggaaa taattggaaa ggaaggtaca ggtgaaggag aattttcctt tcctgaaggc
     1981 ctctggatag atgagggtgg caggttgtgg gtagctgatg ctggcaataa ccgcctacag
     2041 gttattgatg ttaattttct aatcaatccc aaactttatt tagcttcttt attggaagag
     2101 aggaaagacc aggaatttat gcaggtgatg gagagacttt ctcctgaaga taaaaaagac
     2161 ccggaagtag ctcgtctatt gctggagttc tttagaaaaa gaggaaattt agaccaggca
     2221 atttatcaag cagaggactt gtttttgaat gatagtgaaa atcgggatta ctggaagaaa
     2281 accttaggag aactttatta cgaaaaaggt gttcatttgc gaggagaggg aaaattaaaa
     2341 gaagcacggg agtttttcca taaatcctgg caaaatggca atagccattc ccttttcctt
     2401 tatctctgga cttcttttct ccttctggga ggctccaatc tttttattct tattttggca
     2461 atagttctct tatttttgat tatgatattt tctcgtttga gaaaagaacg ttatagaggg
     2521 tggtaactat ggtgaggaaa gcggcggtag cggggtattt ctatcctggt agggagaggg
     2581 agcttcgcca gatgattgag gagctttttt attctgagtg gggtcctggt gggaaggaag
     2641 aggagttaaa aaagtggaaa gaggtaagtg gagtagttag cccccatgct ggttatattt
     2701 attctggagc agtagcttgc tggtcgtttt attctctttc tttagcttct ccggtagata
     2761 ccatagttat aattgggcca aatcatcggg gagtgggtaa aaaagtagct ttgagcgata
     2821 ctgatgtgtg ggaaacacct ttgggagaag tagcggtaga cctggaagca gtggaatttc
     2881 ttgcttctca ttatcctctt ttttctcccg attcccgggc tcatcaactg gagcattcta
     2941 tcgaggttca gattccgttc ttgcaatatt ttcttcctta tccctttcaa attttacctg
     3001 ttactgtttg ggaccaaact ttagaagtgg ctttagaaat aagccaggct ctgcaggatt
     3061 tatccctaaa gcgcaagata gtgatagtgg ccagcagcga tttttctcat tatgagccag
     3121 ctcaggtagc agaagcaaaa gatagccatg ccatatctta tattgtttct ttgcaaaccg
     3181 aggagttcta taaagtgata aggagggaaa atataagcat ttgtggcccg gcaggaattg
     3241 ctgctttaat ggactttcac aaaagctgtt cacgggggga agggaaactt ctcttttact
     3301 cccatagtgg gaagataacc ggtgactatc aagaagtagt aggatatact tctctcattt
     3361 tccctgttgc tacggactaa aagccagggt atcggtcttg ggggactaat ttgttctaaa
     3421 tttttagtaa gaagttttta gtagttttta ctggattctg gaatttatag agtactaaaa
     3481 gtcaacttta aggaggtatg gtgataatgg cagctaccaa attgggtatt attggagcag
     3541 gaagtgcagc attttccctg agattagtgg gagacatctg taaaacgaag gagttatctg
     3601 gtagtcttat atcctttatg gatattgata aggacaggtt gaacgcagtg tatatcctgg
     3661 ccaggaaata cgctgaagaa ttgggagcta atttgaaatt tgaaaccacc accagcgtgg
     3721 aagacgcaat aaaagactca tcttttgttg ttaatacagc actggttggt ggacatggtt
     3781 attttgaaaa agttagacaa atttctgaga aatatggtta ttatagaggg atagattctc
     3841 aggaatttaa tatggtttct gattattata cgattagtaa tttcaaccag ttaaaattta
     3901 tgtatgacgt ggcaaaaatg atagagaaat tttcaccaaa agcctggctt ttgcaagcag
     3961 caaatcctgt ttttgaactg actaatttga tatccaggaa tgttcctata aacatggtgg
     4021 gtatatgtca cggtcatcat ggtatagacc agataataga ggttctggga ttggataaaa
     4081 ataaagtgga ctggcaggtt gccggagtaa accacggaat atggctgacc aggtttttgt
     4141 acgaagggga agatgcctac cctctgattg ataaatggtt tgagaaagga cttgtaagtt
     4201 ggaaaccaac aaacccattt gatgaccagc tatctcccgt ggcgaaggat atgtatgaat
     4261 tctatgggaa aatgcctgtt ggagacacaa taaggaacgg aagctggaaa taccattata
     4321 atctcgaaac taagaaaaaa tggttcggtg aaccatgggg aggagtggat tcagagttag
     4381 gctggaagtg gtatcaggaa agacaggcag aagttgcaat aattatgcaa aaagtggcaa
     4441 agtactttca agaaaatcca gaggcaagat tgttatctca gaaaacatat gacgaaataa
     4501 tttctcttgc taaaggtgaa gttaaagagg gatttgcaca ggaagtattt agaattttag
     4561 atccggaaag aaaaagtgga gaacagcata ttctgttagc caatgcttta ttaaataatg
     4621 agaaagttga tcttgtttta aatgtactta ataaaggaac aatcccgggg attccagacg
     4681 atgttgcggt ggaaatcccg gtttatgcag ataaagatgg gatacatcct taccagatag
     4741 aaccagatat cccggaaaga attaaaaaga tgtaccttat gccaagaatt ttaagaatgg
     4801 agtgggcttt ggaagctttc ttgaccggag atagaagagt cttggaagag ttcttaataa
     4861 gggaccccag aaccagatct tatgaccagg tggttaaagt aatagacgag atacttgctc
     4921 ttcctgaaaa cgaagagatg aagaaacatt attcaaattc ccgaaaatag cacaatttct
     4981 tttgagagac gaggtaaggg actgataatt tcgaggagct atcttaaact ggtttatagt
     5041 atcccgcaaa actggttaaa atattaaaac ttttaaaaag taaattacac cggaagtcaa
     5101 aaactcaagg ttgttacaca cagagaaaga caaagtcttt ctctgtggcc tttatttctt
     5161 ttgttttttt aagttttctt aatcttgtgt cgcagactgc ctttttctcc agcatactgg
     5221 tggatatttt cccaccagca gtttttgttt atttgctttt tgggggttat aatggggttt
     5281 accccactga gggatgtttt ctccctcagt agtctttgct ttttaagttt cttatctttt
     5341 tcctttatct tttttggggg actataaaga ggagcacccc tttatagtct ttataataga
     5401 ttttgcggtt aactataaag attgttttaa gagcgttttt aaatcttcat ctaaaaaggt
     5461 ggcgtgatat tttaggactc tatcaaagat gaatattata gatataagag ttctagaatc
     5521 tcttttattg tccttgagga agttgttttt ttgcccggta aaagctctta ttcaagggtg
     5581 gagttgtgta tgctttttta accttttcgg gggatgaacc ccttatactg gaagatcttt
     5641 tctctccaag tagtcttttg ttttttctta ttttaccgcc tttttccttt ttcctttaca
     5701 gggagctagg atggggtatt gttcttccta gattcctgag gtttttatca ggaatctaaa
     5761 tgttctttaa aaacacagac ccggcttaaa cattgctggg tgacggaaga taagaagggt
     5821 agctcaagac ttacaaagat agatatgctt cttgagtttt tcaaacagcc tcacttttcg
     5881 ttgacaaatc ttatttaata ttgtaaaatt ttctaaactt tagaaaattt tacaaaggtg
     5941 aaaaatggct aaattaaaag aaatagccga gcgagcgaat gtctcagttg ctacagtttc
     6001 ccacgttata aacggtactc gtccggtgag cgatgctctt cgcaaaaggg ttttagaagc
     6061 tatgaaggaa ctgggggtgg aagtaagcgt ctctcaccct aaaaatgagt catcacatct
     6121 cattgccatg gttatagatg atatattcaa cccttttttt attgaagttt ttgccgcagc
     6181 ggaaagcact gctcgcacta tgggttatca tcttcttctc ttgtctatag aaaaagacta
     6241 tgacactttt tatcttcagg atctgggaga taaaaaagtt gatggtttta tattggctac
     6301 tcgtctgagt ttgtcttacc ttaaagaatt tttttctgct caaattcccc tggttttcct
     6361 gggaggagtt ttagatagtc ctttttctcg tagtattctt tttcccgatg aagagggggc
     6421 ttatcttgct actcgccatc tggtagattt agaacattcc cggattcttt gtgtaggtgg
     6481 cccttctaag agctctcttt ttcgtaaccg gtttcagggt tataagaggg ctttgaggga
     6541 aaaacaaatt cctgtagacc cacaattaat ggtagaaatt actcctactt ttgaagaggc
     6601 tcgtcaattg gggttaaaat atctttctct tgaaaaccag ataagtgccg tttttgctca
     6661 taacgatccg actgcttgtg gaattatggc tgccgctcaa gagctgggta gaaaggttcc
     6721 ggaggattta tcaatagttg gttttgataa tacttttttt acccagttaa cctgcccccc
     6781 cttgacctcg gtagaaattc ccaaaaaatt aatcggtaaa cggtcggtgg agctattgat
     6841 tcgcctgatg aataatgacg gagtgcagtc ctatgtaatt gaagaggcga agctaataga
     6901 gagaaaatct actgcctttt ttcggaaaaa taacctgggg gtgtaaagat tggataaaga
     6961 agcaaagata acggtagttg gagcagggag tgcagctttt ggtctgtcaa ctttggtggg
     7021 gatactgcgt catcctgatt tacaaggagt ggaactattt ttgcacgaca tcaatgaaaa
     7081 tggtttagaa aaaatcagaa aattggccga gaaaatgaat aatgcctggg gctccggtat
     7141 gaaaattaac agttccgtag agagagaaaa ggctctagaa ggagcagatt ttgtagttct
     7201 ttccattgct attgaccggg aaaaatgctg gagattagat cgagatattg ctattcgcta
     7261 tggtatcaat cattatgctg agaatggagg accaggtgct tttgcccata cggctcgtaa
     7321 tttgtcagct attatgccca ttcttcagga tatggaaaaa tattgcccgc aagcttggct
     7381 tttgaacttt actaacccgg ttcccaagat ttgcaccgcg gccaatttat attccaatat
     7441 aaagaccatt ggcatctgcc atcagattaa ttttggttat tttatcttag ctgctctttt
     7501 tgccgatgag ctggatttac acctccctcg agactttaac tttcgatgga atgaccaatc
     7561 tctatctctt tttcagatta tgagtaaaaa agctaaagaa aagtttctta tccgagcctg
     7621 ggggcttaat cattttacct ggatggtaaa tgtgacggaa aaggggacgg gaagaaatgt
     7681 gtatcccgag atcgaaaaaa gaatggaaac tcttcctcct tcttttgagc ctctgaccca
     7741 ggaagttttt cagatttttg gtcttttacc ggttcccggg gattgccatc tctgtgaata
     7801 tcttccttac actcataata taaatcgtaa aacctgggaa aggtatgaca ttcagatgta
     7861 cgattttgaa tgggcggaga aagggagaca aaaaatgtgg gaaaaaattg atagagtagt
     7921 agaaggaaaa gaatcactta aaggatggga agaagtagaa acagaaagag gagagtttat
     7981 tattgctggg atattgaaga acctcaattc ttatgaagaa gcaattaata ttcccaaccg
     8041 gggatatata gttaatttac ccgatgaagt gataatagaa gtcccggcga ttttaggtag
     8101 tgaaggcccg gtaggtttag gaggatgtga acttcctgaa cccattgctg aactgtgtcg
     8161 aagacagtct cttataaacg agttagtggt aagggctatt gtggaagaag atcgaaaatt
     8221 ggctcaagag gcttttgctc tggatcccat ggttgatgac cttgaagtag cgaaaaaact
     8281 tctggatgat tacctaaaga cttttgattc ttatttaagt tttaagttat aggaggtgat
     8341 gcggggaaat ctggctaaaa tagaaagagc tggtaagggg aagtcgtgta gtaattggtg
     8401 gtaaggaaac tttttagtat agtgtgattg attttgaaaa ggaggaaaaa gatgaggaaa
     8461 atagtgtggt cggtggtgct ggctattttg ctggttggtg cagctggtgt gagctttgcg
     8521 caagttttac cggagattcc cagaactgag actctcatcg ttgatatctt acatggacgg
     8581 attggaaacc caggaaattt taatgtttgg attccaggat ctcaagccgg tcatgggctt
     8641 caacaaatgt tgatggatgc tttatggtat gtagaccctc agacaggtga atggattaat
     8701 gctttagccg cagaagagcc aaaatataac gaagacttta ctaagatgac gataaaaatc
     8761 agagaaggaa tttactggag cgatggagag cctttcactg ctgatgatgt tgtatttact
     8821 gttaaaaata ttcaaagcca tcctggaatg aactctcagc ctcaacttca agagtacgtg
     8881 aaagatattt ataaaacaga tgattataca gtagaggtgg aacttaccaa atcatattct
     8941 agaatgcata acatatttac ttctctggtt tacggagctc ttttcatgca gcccaaacac
     9001 atatgggaga aagttgagaa tcctctggct tttgatttca acccccctgc ctcgctgggc
     9061 ccgtatgtac tcgatagtta tgatcctcag ggatactggt tcttgtttaa aaaacgcgat
     9121 gactggcaaa ggacaagcgt gggacaattg tatggcgaac caaaaccaaa ctatgtactc
     9181 ttcatttact acggtgcaga tgatagaaaa gtgatggccc aagccagaca tgaattagat
     9241 atgatcttcg atttaactcc tgaagcatgg gaggttttaa gagaaagaaa tgaatactca
     9301 gtagtgtggt acaaagattt cccgtgggca tggatggacg atgtaaactc aagaggaatg
     9361 tttcttaata atgccagata tccttttgag attaaagatg taagatgggc actaactctg
     9421 gcagtaaatg tagaagaaat tgttatatca ggatatgcag gaatagagag agtatcatct
     9481 ctgcattcct gtcccactca atattttaca gaaatttact ttaaaccgtt agagccgtgg
     9541 ttagaggatt tcaccataga tggtgactat aaaccatatg atacagacgt agcttataga
     9601 attgcagagt gggctgaaaa gcaatataac ataaaggtag aagatcctga agctgtctgg
     9661 ggaatcggtt ggtggaaata tgatcctcaa aaggccgaag atttactttt gaaaaatgga
     9721 ttctctaaag atcaaaacgg aaaatggcta ttacctgatg ggactccctg gaagataagt
     9781 ataatagccc ctgctgattt tgaaatagat gccaccaggc tcgcttttgc agttgcagat
     9841 cagtggagaa aatttggaat cgaggtaaat gttgaaactt tagaatcagg tccatttttc
     9901 tccagacagg ctataggaga ttatgatgta gggtctttct ggggtgggat gttagcctct
     9961 tctggtattc cagatatatg gacgtttata aatggtttta aatccgaatt cttgcaacct
    10021 ttaggaactg ctgtgactac cgcaaataat taccgatgga aagttcctga gagagtggat
    10081 caaataatag atgagatggg tactttgtat cctggagatc ctaaaataat agagctggct
    10141 caagaatttt acaaactcgc agtagaagat atgcccacca ttcctatggt aataaacaaa
    10201 aaatttaacg ctaacgacaa ttattactgg acaaattacc ctagtgctga taacccttat
    10261 atggcaaatg tttgttggtg ggctgaattc aagtttatgc tccccaggat tgaacccaca
    10321 gggagagaat agattctctt aactcgttga gcccgctcaa cttgagcggg ctcaatttgg
    10381 aaacaaggat tttaagtttt aaggataaaa tctacaagtt ggaaagccta aaattgagtg
    10441 ctgatgggta taaaataaac tataatatat tttctggagc agattgtaag actccagaca
    10501 caggactggt tagaaaagtg gagggaaaaa ttttgtgaat gccttaaaaa cttatgttct
    10561 tcctcgctta attcagtact tagtagttat ttttgttgga ataaccataa cttttataat
    10621 tcccagatta actcctatag accctgttca aagcgctatt aaccgcctga catcatacgg
    10681 agggatatat atggagccac aggcggttga gagcctacaa aatactttaa aagaattata
    10741 cggattagaa ggaagcttgc tggaacaata ttttgccttt tggagacatc tttttactct
    10801 ggactttgga ccatctttat ccatgtttcc agctccggtt atagaaatta ttgcaaattc
    10861 tctttcctgg actgtttttt tgcttttaac gactttgcta atatcctgga taattggaaa
    10921 tatccttgga gggttagcgg gatattttaa agacaaaaaa tggtctaacg tgttagctat
    10981 tatagctatg agcatatacc ctattccata ttatgttatg gcattgattt tagtaatttt
    11041 gtttgtatat gtgtttccgg tgtttccgat gatgggaggt tatagcatag gagtgcagcc
    11101 ttccctaact tgggctttca ttttagatgt tttaagacac agtatattgc ctgctatttc
    11161 tctcataata attggaattg gctggtggtt tttaagtatg aggtcaatga catccaatgt
    11221 ggtagctgaa gattatgtta cctatataga atttgcaggt ttacccaaac ataaaatact
    11281 ttttagctat gtcatgagga atgccatact ccctcaagta actgctttgg ctttacagat
    11341 aggaggtata tttaatggag cagttataac tgaggtagct ttttcttatc ctggggtggg
    11401 gttgttatta aaaaatgcta ttactaatgg agactttaat ttgatgatgg gaattgcggt
    11461 tttctcaata atagctatag ctactgcttc actggtgata gatttagtgt atcctttgtt
    11521 tgatccaagg ataaggtata gataaggtga tgggatgttt gtcatattaa aagatctctt
    11581 taaaaaagat agaaaatttc ttttcggttt taccgtgata tgtattttag cctttttagc
    11641 tatattatca tttttttctc cctatgatcc cagaagttgg agtgtggttc ccaaagatag
    11701 acctcccagt tttcaacata ttttagggac caattcaatg gggcaagata tattttggaa
    11761 tactactcat gctttgaaaa actcttttac acttgggata acaactgctg tcgtagctac
    11821 tattatcggt actattgtgg gtctgatagc agggtataga gggggaacat tggatagatt
    11881 tttaatgtcc ataaatgata gtttcatagt tttgccctct ttgccgattc ttgtctttct
    11941 ttccttttct ctaagagaaa agatgaccat gtttgttctg ggattaacgc tttcggtatt
    12001 ctcatggccc tggggaggta aacaagttcg ggctcaggta ttaagtttaa gggagagaga
    12061 atttacttac acatccgttt tttcgggaat gaatatgtgg aaagtagttt ttcgggaaca
    12121 cttacctttt gttattccat gggttattgc aaattttata aatacgatac tctgggcgct
    12181 gggaatggaa attaccttat cagttctcgg cttaaccagt ttggaaactc caacgattgg
    12241 aacaacgatt cactgggcta tgcaatatca ggccatcttt agaggaatat ggtggtggtt
    12301 acttacgcct ataattcttt gtgttttact ttttattgct ttgtacatgc tttctgtgag
    12361 catcagcgaa tttttggatc cgagaactag attacaaagg ataagaatgg gaggataatt
    12421 atagatgtcg cttctcgaag tggaaaatct ctctgcgtat tacatcaccc acgcttatgg
    12481 gataaaaaga gatgttagag cagtagataa tattacgttc tcaattgagg aaaatgaaat
    12541 tgtggggtta gcaggggaat cgggttgtgg gaaaagcact ttaattaaag tacttttagc
    12601 tataataaaa cctcctctcc ggattttgag tggtcaagtt aactattctt atgatgataa
    12661 aaaatttaat ctcctgaccc tagaggggaa acaattaaga agggtacaat gggaagatat
    12721 atcctatatt ccccaaggct ctatgagcac tcttaatccg gtaagaaaaa ttagaaaaac
    12781 ctttgaagac gttataaagg ctcataaaag aggtgaaagc aaggaagagt atgaaaagct
    12841 catggaagaa catataaaat atctgggtct tcctctggag gttttagatt cttaccctca
    12901 tcaactctct ggaggtatga gacaaagaac tacaatagct ctggcaacta tcctgagacc
    12961 taaaataata tttgctgatg aaccaaccac agctttagat gttgtggttc aaagaggagt
    13021 aatacaaatg cttaaaaaat tacataaaga acaaaaaaac acgatgctga tagtaaccca
    13081 tgatatggca gtgcatgcca atgttgccga aaagatagca gttatgtatg cggggaaaat
    13141 agtggaaata gctgagaaaa aagagctatt tactgatccg aagcatcctt attctcaatt
    13201 tttaatacag tccctaccta ggattggaga caagaaacga aggactagtg ctcccgggaa
    13261 ccccccatct ttagctaatc ctccctctgg atgtcgattt catccccgat gtccttatgc
    13321 gaaagatgtg tgtaagaatt acgcaccgaa gctcataaag tatgaggaat ttcataatat
    13381 agcttgtttt ttatactcct ctgaaacaga ggaaggtgag aaaattgtcc aggaagttat
    13441 tagaagttaa aaatgttgta aaaatctttc atataggggg aagattttta ggaaccaaat
    13501 taaaagcagt taataatgtg agttttgaaa tagattctga aaaacctgaa atattaactt
    13561 ttgcaggaga gacaggtagt ggaaagacaa ctctggccag gatgattctg gggttggaac
    13621 caccaacttt gggagaaata acatacaaag ggaaaaacgt caccaatatc aaaaaaaggg
    13681 aagaactgct agagtatatg aaggaagtcc agcccatatt tcaaaatccc ttcgagactt
    13741 ttaatcctct gagaaaagta gattcttacc tttttgagac agcgggaaat tacaatttag
    13801 caaaaactag gaaagaaaaa atgaaagtag ttgaggaagc cttaaaatca gtgggactga
    13861 ctttaaatga ggtacaggat agatacccga atgaattatc aggaggacag ctacaaagaa
    13921 catcggtagc cagagcctta ataaccagac cttctttgct gatagccgat gagcctgttt
    13981 ctatggttga tgcatctttg cggatgtcta tagttaactt atttagtgat ttgaaaaacg
    14041 attacaacgt tactgtttta tacataaccc atgatcttgc cactgcatat tatataagcg
    14101 atagaattgc aataatgttg agaggagatg tagtagagtt tggagatatt gacaaagtta
    14161 tgctttctcc actccatcct tatacccagg tactattgaa atcagtacct caaccagatc
    14221 ctgagggaac gtggaaagaa gatataaaac tatcttcttt ggaggtaaag gagttttcaa
    14281 ggttgggctg caaattttac gaaaggtgtc cttcggctat gaaaatctgc aaggatgttg
    14341 aaccgccgaa cgtttttaaa aatggaagag atgtaaaatg tcatctgtac tcccagaaat
    14401 tggaagaaag agaagaagct tgaagagatt tactgtaggg aagtaatatt tgcgtggtaa
    14461 aattagaaaa aaatggggag gtagagaaat gcagtatata gagctatatg aagctcgagt
    14521 tccggttatt ggtttaggaa cctgggattt acgaggagaa atttgccaga gggcagtatt
    14581 aagtgctctt aatctgggat atcgtcatat cgacactgct caatcttatg ccaatgaaga
    14641 agagatagga aaagcactac agagttctcc agttcctcgg gaagagattt tcctcaccac
    14701 taaagtttgg tatactcact tgcgttattc tgaagttatt caggctctgg aagaaagttt
    14761 gcgaaaatta aaaactgatt atgtagacct ctaccttatt cattggccca gtgaggatgt
    14821 tcctttagag gaaaccatga gcgccatgat taaggtaaag gaggaaggaa aaaccagata
    14881 cattggagtt agtaattttg atgttcctct tttagaaaag gctcgacaaa tttctcctta
    14941 tcccattctt actgaccagg tggaatttca tccctattat tctcaaagag agcttctgaa
    15001 ctattgtcaa aaaaataaag ttattcttac tgcttattct cccttagcga gaagaaggtt
    15061 gagtgatgat ctgttgttac aggaaatagg caagaaatat ggtaaaacgg ctaatcaagt
    15121 ggctctacgc tggctcattc aacaggagat ggtagtggct atccctaaag ctgccagccc
    15181 tgagcaccag agggagaatc tagatatttt tgattttgaa ctcactccgg aagaaatgga
    15241 gaaaatttct cgtttagacc ggagagaaaa agtagcttct cgtatctaaa atactcatca
    15301 tttctggaaa gtttcaaaca ctctgaagaa taaaaattag cgggtaatgg cctcttttcc
    15361 cgggggataa gaaaatcggg tagtattatc ggataaaggg cagctctcgc aggtagagtt
    15421 agcaagatcg gttattcgat agttaatctg aattctttgg tcttcttttt taagcagaaa
    15481 aaaggaaaaa attatactga tggctactcc taaataatga tataaagtta gaggagtgag
    15541 ataacgaagg agaaaataaa caaaaagagt aagtaagata ggaaagatgt aaattaaaaa
    15601 agctattcct aaagctgcat ggtcttttat ttctactaat actctatctc ctggggaggc
    15661 gcttaattca ttataagcct taaccatgta aaactcactt ttagagtcat caataagggg
    15721 agaggagagg ggacactttc cttctccaca agaagttccc ctctccattt ttacccagct
    15781 atattttcct tcggttttta ctacttgtcc aattcttttc atttttactg tagaccaatt
    15841 aaaatatctt tcccttctac tttgacctca tagactggta aattctcttt agctggtcct
    15901 tttactacct gacctgtggt tatgtcaaag tgagagccgt gatagggaca ggtaattata
    15961 ttttcttcta ttttcccttg agctaaagag agacccttgt gggggcagcg gctaccgata
    16021 gcataaattt tccccttcga atttaaaaga aggattttat tttcttctat agtaatttct
    16081 tttcttcctg acgacaattc taaaatttct gctacctttt taaactcggc catgtttctc
    16141 catcctcttt attggttgta aaagtctatt aaaaataaat ctctatttac agagacttat
    16201 ttccaataaa gaacaccttt taagaaatta tattccagac ctgaagtaaa agtgccttcc
    16261 tcattagctt ttgcttttat aactttttcc gctttttgat cttgcgttgc tgatacgcca
    16321 ggtatttttt cggtgttgct attttaattt cttaatcttt tatgcttttt gttttacatc
    16381 ttaagttatt gtttatttgc taggggagtt aagctataat atctcaagag tatacaatgg
    16441 ttactatttc taaaggaaga gtatttcgct tttatattga atctcgatat ccttttgggg
    16501 aattcgcttt aatccgttct cgggggaacc tgggagaggt aggctttgtt ttagctgatg
    16561 aaaatgtgga gcgttggccg tctctttacc ggaaagcctg ggaaatagcc aattatttcc
    16621 gtttaaggat taaagagtta ggtctggata atatagcggt ggccggggta ggaagagatt
    16681 tagattttat cactgcttct atcataatag acctttctgc ttttgagaaa gaagacatag
    16741 aaaaggttgc cgatcagatt atgggtattt tgaaagaagc taatccttat tataataata
    16801 aaactggaga gattgatgag tgaaaaagta ggatttatta ctatgggtga gtcaaaggga
    16861 atagtagaga accttcctga gttaagagta ggaatgttag gttatgcttt tatgggtaaa
    16921 gcccacacca atgcttataa aaaataccct tacattttct ggccaccggt ggcaattccc
    16981 agattgatag ccatttgtgg gaggaatgaa aaagcagtga gagaagctat gatacgctat
    17041 ggttatgaaa aatactacac cgattggaga gaaatggttg aggacccggc agtacaaatt
    17101 ctggataata gcagtcctaa ttatcttcat gctgaacctt ctattttagc tttagaaaaa
    17161 gataagaatg tcctttgtga aaagccttta gctggtaatg ctcaagaagc taaaaagatg
    17221 tgggaagcag ctcaaaaatc cagtgctaaa gctatggtgt gttttaacta tcgttttgtt
    17281 cctgctcttc gattggctaa agagtttatt tcttcgggta aattggggga gatatatcac
    17341 tttcgggcgc aatatctcca ggagtggatt atggaccctg attttcccac aatctggaga
    17401 ttggataaaa gtgctgccgg ttcaggagct ctaggggatt taggagctca tattatagat
    17461 ttagctcgtt ttttagtggg agaaattact acagttagtg ctttaactcg tacttttatt
    17521 aaggaaagaa aggtagcagg agacccgggg aaaaaagttc ctgtaaccgt agatgacgct
    17581 tttgtagctc ttgtggagtt tgaaagggga gccataggaa cgctggaggc ttctcgtttt
    17641 tgtgcaggaa gaaaaaatta ccaatttata gaaattaatg gttctcgagg tagtatccgc
    17701 tttaacttag aaagattaaa tgagctggaa gtttacctgg taggagagga acccaaaata
    17761 tttcaggggt ttcatgatgt tttagttact gaaagcttcc atcctttcta tgaatattgg
    17821 tggcctcaag gccatattat tggttgggaa cacactttta ttcatgcagt ggctcatttt
    17881 ctagatgcta tagttaataa taaagatgtt gctccttatg gagctacttt tgaggatggt
    17941 tatcgatgtg cagtaatctg tgataccata ctccagtcgg cggaatcagg taaaagagaa
    18001 gcaattccac aggattgaaa ataaacttta tttacagggg gaactatgaa atatcgaggt
    18061 aaagtaatag taactttgaa accgggagtt tttgatccgc agggggttac cattcgtagt
    18121 gctttgcata ccctgtctta tagggaagta gaagaagtta aaactggtaa gtactttgaa
    18181 atagttttag aattagaaaa tctttcgcaa gcagaaaagc gtcttcaggg aatgtgtgat
    18241 caactcctat ctaacccggt aattgaaagt tattcttacc agatagaaga aatgaaagac
    18301 tgaggatgaa aatgaaattc ggaatagtgg tttttcctgg gtctaattgt gactatgatt
    18361 gctatcatgt tttgtctcag gtcctggggg tagaaacagt gtttatctgg tatggtgata
    18421 gaaacctgaa agactgtgac tgtatagtat tgcctggtgg gtttagttat ggtgactatc
    18481 ttcgagccgg agctattgcc cgtttttccc cagtgatgga aaaggtaaaa gaatttgccg
    18541 aaaacggggg gttggttata ggaatctgta acggttttca ggttcttact gaagccggga
    18601 ttttacctgg agctcttctt cccaatagag atggacgttt tatctgtcgc catgtctatt
    18661 tgagagtgga aaataatcag actcctttta ctcttttatt tactccggga gaagtagtga
    18721 aaattcccat tgctcatcag caaggtaatt tttttattga ccctctttgt caggaagaag
    18781 ctaaaaagca ggtagttttc cgctattgta gttctgaggg agaggtgggc tctcgttata
    18841 accccaatgg ttcctgggaa aatgctgcag ggattatctc gagggaaggt aatgttctgg
    18901 ggatgatgcc tcatcctgag agagcagcag aagcgattct gggttcagca gatggtcgaa
    18961 gggtgtttct ctcagttata aagtggtggg aggagaaaaa gtggaagtaa caaatggcgc
    19021 aaatattgct aaagaattgg gtttgagaga ggaggaatac gaaaggattc ttactatatt
    19081 agggagagtt cctactccta ctgaaattgc tatgttttct gtagaatggt cagaacactg
    19141 tggttatcct cattcccgaa agtggttaga gttgcttccc cgagagggtg agttttctcc
    19201 tcttctggga gaggatgctg gcggtatagt ttatgatggt ttagctatag tattcaaaat
    19261 ggaaagccat aaccatcctt ctcagataga acctaaacag ggggcagcta caggaattgg
    19321 aggtattatt cgagatatat taggaagtgg agcgcgacct attgcttgcc tcgattctct
    19381 gcattttggc cctttggaag atcagcgttc tttgtatgta tatcggggag tagtagaagg
    19441 aatagcgttt tacggtaact gcgtaggagt acctacggta gccggagaat tgtattttta
    19501 tcctgctttt cgaggaaatt gcttggtaaa tgtaatgtgt ttaggtattg ctccccggga
    19561 gaacttaact caggctaaag cacaaggaga gggaaattat atcgtttatg tgggtaataa
    19621 aaccggaaga gatggaatag gaggatgcag tatattagct tctcaagaat tcggggtaaa
    19681 agaagagaaa aggcctagcg ttcagattgg tgaccctttc gcagaaaaat gcttgattga
    19741 ggctactctg gaagctctga ataccgggta tgtggtggga gttaaagata tgggagcagc
    19801 aggccttacc tgttctacaa gtgaaatggc tgccagtgga gaaagtgggg tagaaattaa
    19861 tctggattta gttcctctcc gggaagaaga aatggaacct tgggagataa tgatgtctga
    19921 atctcaagaa cgaatgctcc tctgtgtcag gagaggttat gaggaagata ttattcagat
    19981 tttccgaaaa tgggggttag aaggagaaat aataggagaa gttaaagata caggtagagt
    20041 gacaataaag tatcagggag aagtggtggc tgatataccg gcactagaat taactaaacc
    20101 tccggtttat actcctcctc gggaaaaacc tctttattta gatgaggtca ataatttttc
    20161 tctagaaagt attcccctcc cttcagatta tgaagaaatt ttatggcgta tggtttcttc
    20221 agaaaatctt tcttctcgtc gtcctgtttt tgagcaatat gaccatatgg tgggaattaa
    20281 taccgttctt ctccctggtt tgggaactgt agtattgagg gtgaagggca aaaaatgggg
    20341 aatagcagtt actactgatt gtaatccact ttattgttat ctttctcctc gagaaggagc
    20401 taaaatggcg gtggctgaag cagcccggaa tttgagtgct tgtggagcta aacctgccgg
    20461 agttaccgat tgcctgaact ttggcaatcc tgaaaagcct gaccgttatt ggcaatttgt
    20521 ggaagctatt gaaggtttaa gcgaagcctg tcgttttttt ggtattccgg tagtgagtgg
    20581 aaatgtttcc ttttacaatg aaaatcctca aggaacaatt ttccctaccc ctaccatagg
    20641 aatggtggga ataatagaag atgtagaaaa agtagttagt gctggattca gagaagacga
    20701 tttaatcgtg gttcttctgg gtgaaaccaa agaagaactg ggaggaagcg aatatctccg
    20761 ggttatccat catcgagaag aagggccccc tcctcaagta gatttgcaaa gagagaaaaa
    20821 tctgcaagag ctccttataa ctttagtaag agaaggggtt gtttcctcag ctattgactt
    20881 gagtgaagga ggactaattc tggcactcgt agaatcttct gttataaaag aaaaccctcg
    20941 aggagcaatt gtagacctgg aatgtctggg agatatgagg gaagatgttt tgctttttgg
    21001 agagagtgga ggaagggcgc tcgtgagtct gagaggagaa tcgttgagtt ctttacaccg
    21061 cctttccgga gaattaggtg ttccttacca ggtgatagga aaaactgggg gagagaaatt
    21121 aattattaaa aagggagaaa aactacttgt ttcattaccg gtagaggagt tgaaaaactg
    21181 ccgggagaga ggtttgggag aataagataa ggtggatgga aaagaaaagt gtggtatatt
    21241 tgggatatat ggtctgaaga atgcttctga gttaacttat ctgggtcttt tttctcttca
    21301 gcaccggggg caagaaagtg ctggtatagc agtatcagat ggaaaaacag tgcgagagaa
    21361 aaaaggaatg ggtctggttt ctgaggtttt tactcctcag agccttaaag gtcttcctgg
    21421 ccatatcgct ttagggcatg ttcgttattc tactactggt tcctcttctt ctcgtaatat
    21481 tcagcctttc atcggagaga gtcgctttgg tcatttagct attgctcaca atggaaacct
    21541 gaccaacgct ctggctctgt ggaaaaaatt gagtgaaagg ggagcggtat ttcaatcttc
    21601 catggatact gaactcattc tccatctcat agctcaaggt tctttcgatg gtttagaaga
    21661 ttctctgaaa gacgccctgt ggcaaataca gggtgctttt tctctgggga ttctttcttc
    21721 tcatcaacta atcgctgtgc gagacccctg gggttttcgc cctctgtctt tggggaaatt
    21781 aggtgatggc tatataataa gttcggagag ttgcgccttc aacgtactgg gagcagaatt
    21841 tgtgcgggaa attgagcccg gagagatgat tataattgat gaaaaaggat tacgttcttt
    21901 tttttatgct tattctcctc gcaaagcttt ttgtattttt gaattgattt actttgctcg
    21961 tcctgatagc gtggtattta gtaaccaggt ttatcaggct cgcaagaaca tgggcaaagt
    22021 attggccgag gaagaagatt gttcagcaga tatggtagta cctattcctg attcagggac
    22081 ttttgctgct ctgggatttt ctgaagcatc cggtatacct ttagaatttg ccatggtacg
    22141 taacccttat gtaggaagaa ctttcataag accggaacag ggagcaagag atttggcggt
    22201 gaaaataaaa cttgatccta ttagagagct cattcggggc aaaagaataa ttgttattga
    22261 ggattctata gtgcgaggaa gtacttctcg tcagagaata gctaccttga aggaagcagg
    22321 agctcgggaa gtgcacatgc gggtgagttc cccccctcat attttcccct gtcattatgg
    22381 tattgatttc cctacccgga gagaattgat agctagtgat aagtctatag aagaggtaaa
    22441 gaaatattta ggtctggata gtttaaggta tataactatt gagggcttaa aaaacagcgt
    22501 aacccctccg ggagaaaatt attgttttgc ctgttttaat ggtgattatc cagtcactcc
    22561 tcaagaagga ggaaaactga ttctggaaaa aagacaggtt atcaaccaat gttagccaat
    22621 gttagtttcc tccttcttgt tttttaaagc gtgacaggct caggcaggtt ttagtgattt
    22681 aaggagccta gctagttctc gagcataaag ctggcaatct ttttttgctc catcatcaaa
    22741 agtgcttact gctaccggtc catagtgact tccctcagcc actcctttta ttaccatccc
    22801 gtgaattaaa agagcggata gaattgacca gatggtagtt tcgttgcccc ctgcccggtt
    22861 agcagaagaa gaaaaagctc ctcctatttt accgtttaaa tcaccatgaa gtttgatgct
    22921 ctcatctaac atttctttga tttctgcagc cattgttccg taataggtag gagaaccaat
    22981 tactatcaag tcatattttt taaagtcatc tacttgaaac tgagaaacat caactaagtc
    23041 ggtagaaact ccttcttgac tcagagttcc agcaatggtt tgagccattt tttcagttat
    23101 tccagtacgg gtgtaataaa gcactaaagc tagtgacata tgatttttcc tctctctata
    23161 aatagtcaaa catgcttttt atggaaattt ccccttcaat atctttgata attttttctt
    23221 tacccgggaa cagagcaaag aagttttctc cagagtagtt ttcgtcatta actcttatat
    23281 ttactcccag agctggtttt tcgcatttta atactaggtc ttttcccgct ttttcataac
    23341 ttattcgggg gtcttctaaa tgatggagtc tcaaatcggc aaatatttca tggttctcaa
    23401 ttattatttc ttctttttgt tttaatttga gataagctac tgcagattct aaaatagaag
    23461 ggtcgacttc taattgacca actaaaatta ctgagtcttc gggaacttta aagttgcggt
    23521 aggtctcttt cttcaataaa ttgcctttaa gcccccataa ttcaaattct aaatttactt
    23581 cactataacc tccatcgttt attattgaaa taaatatctg tccgtcttta tttttagcta
    23641 tgggaagcaa gctactgtaa aatcttctag tatagaaata taaagctttg ggtcttttaa
    23701 aataatctat ggatgaccag ctaaaaactg gccaggagtc atttatttgc cagtaaagtg
    23761 ttccagcggt tctgtatttc ctacttctcc agtgttctac ccctgcttta atagcttctg
    23821 cctgatttaa ttgggtgaga taaacaagag aatcaaagct agatattaaa ccatagtgat
    23881 tgtttataaa ctttaatagt ctttctggtc cttccacttg tttattatga tttaacatga
    23941 ctggtgaaaa caggttttta tctttttctg aggcaaagaa atctatggtt ttcgcatcag
    24001 gtgcagcttg aaatccgaat tcgctgacaa atttggaggt gtctctatag taatatttat
    24061 aatcttgcca gcccgaccag atttcccata catgcctatc tccagcttta tttgaattgg
    24121 ctcgttcccc tccatagggg ctggaaggcc agtaaagtct tgaggggtct tcttctgaac
    24181 atatgagggg aaagtcttct aaatacaacc tattacctaa gtttttcccg tctactttta
    24241 ttttgtaatc ccattcctca aatccccagt tattttcgtt atttccacac cagatggcga
    24301 tactggggtg acttctgagt tttataacat tatgccttac ctcttcgttg gccagttttc
    24361 tgaaccagtt tatatggtcg ggatattcgg cacaggaaaa cataaaatct tgccagacta
    24421 aaatacctaa ttcatcacaa tagtcataaa aagcagggtc ttcatataat ccaccacccc
    24481 agattcgtag catgttcata tgggcatctt tagccatctg gagcaaagtt ttatagtctt
    24541 ccttggttaa ccagcttaag atattttctc ctggtatcca gttggcacct ttagcaaata
    24601 tttttttccc gtttatctca aaaatgaaac tttcaccttc gccatcgttt tctcttatta
    24661 cttttaccgt tctaaacccg attttctttt tttcggtata tatttctttt tcatttttga
    24721 acaatttgaa ttctgcagga taaaggtaac tttctcctga gccattggga taccacagtt
    24781 ttatatcttt tatttttctt ttcccttcaa acttaaaatt tttaacattg cgggttactg
    24841 gaaagctatc tatgctctta ttatccagga taatttctac tttgtaggtc tcgacttcat
    24901 ttcctataga atcgatatag ccggaaaaga ctacagtccc atctaagtct tcaagatacg
    24961 ctgttgagcc atagattcta gcgtcaggat aacaatctaa gtagacattt ctatatacac
    25021 cactagtggc aattcttgct ccccagtccc atccgtaaga atattgtgct tttcttatat
    25081 atactcgagc tgtttcttcg ccagcatgaa gtttaccata atttttttct aaaatttttg
    25141 gtatctctag gggagattct attttaatct ttaagaaatt ttctccctct tttaaatatt
    25201 cttttaccgg gagtcggtat tcgatgaaca tgtcttcagt ttttcctgca aattctccat
    25261 tcacatacac ttgagaaaga gtatctaccc cttctaatac taaatcgtag ttagtctcct
    25321 ggtctaattt atctatatag aaagttctct cataaaccca ggatttttct tccaatcttc
    25381 taaacagttt ctcgtttgtt tccacataag gatgaggcat taaattcaaa cgtaccaggt
    25441 ctccttgtac tgtacctgga acatcgcctt caaaatcgaa ttccttttcc tcatcataaa
    25501 ctctccattt cccgttcaaa gagattttca aataaactct cctccctcat gtctttttaa
    25561 tttttgtaac tcttagaaga gttactttcg actctaaaaa atatggctcg gattttttaa
    25621 gtttattgta ttatatcatt tggcattttg aatttactga aatattctca ttatagtatc
    25681 ccccaaaact agccagaact taatttttct tgccaaaagt aaactctttt gttaatatat
    25741 gccgtgatta ttgtcacggt tcggaggtaa agagaatgat tgaagaagct atccaggaga
    25801 ttagggaatt ggagaacctg aaagagagag agataagcag tgccgagctg gaggcagcta
    25861 aaattatggc tcaggtagaa gaccagatag ctgagatgag aaaaaaagct ttacaagagg
    25921 tagaagaaag agctcaatct ctaagagcag aaatactcaa ggagggagag gaagaggcca
    25981 agaagataga agaagaattt cgccatcaag ctcaaaaatt agaaagtgct gcttcttccc
    26041 ggatgaaaga catcgtttct cagctggtaa aagagatggg agaagaatat ggcaattagt
    26101 gagatggaga aggtgggcat cattgctcat cactctctac gaaaagggat tcttgaccgg
    26161 ttacaagacc tgggggtttt agaaatatcc gaagatgaaa aagagttatt tgacgaattt
    26221 gttgctcctt caggagaatt aggagaatta gaacgccgac tgggagagct aggctattgt
    26281 ttagatttta ttgataaata tcgctcagaa aaaccaggga ttgtgcagtc tctttttcct
    26341 gctcctattc atatggaaaa agaagatttc ttgagctgta gttttgatta tattcctctt
    26401 tataagacct tcctgggttg ggatgaagag cttaacaaca ttcgtagcaa cttaaatagg
    26461 ttgaattcta acctggagtt tatgaaaaga attcaatcgg ttaaagtttc tctagaaaat
    26521 ttacgaggca ctctttatac tcggagtttc cttttggaag tccctccgga aaggaaaaat
    26581 gttttagagg agaagctaaa ggagaaagat ttgatctggt tgggagaaga atttccgggg
    26641 aaaggtaaaa acacttggtg gtgggtggta atccacaaaa gtggagagga ggctatgaga
    26701 gagattttag ccgagctttc tctttcccct gtgagccttc ctcagccttt tgaaggtact
    26761 cctcaagagg tagtagaaga tttggaacac cgaattactg aacttcaaga ggaacagtca
    26821 aagctcctgg aaaaagcagc tctgcaagaa aaatttgaga aagagcttaa aatactttat
    26881 gactattatt tgagtttgtg ggagcgacac cagagcgaaa ctcggctttt aaaaactaaa
    26941 gagacttgtt atattagcgg atgggttaaa aaagaagact tacctctgat agaagaatct
    27001 ctctctcaag tgggtaagga gtgggttctc taccatagag agccagaaga aggagaagat
    27061 gtacctgtca ttctggaaaa taatgcctgg gtaaataatt ttgcagtttt aactcatctc
    27121 tatgggcttc ctaactatac tgaaatagac cctactcctt ttgtggctgg gtttttcttc
    27181 tttttctttg gtatgtgcct gggggatgta atatacggtt tggtgttggc tctttttggg
    27241 tttattgccg ctgcctatct cgatgtttct gattctacca agacttttat gcgtatgtta
    27301 gcctggggag gagttggagc tattctagta gggatttgta ctggttcgtg gttgggagac
    27361 ctttttgatt atctcccttc ctccttgtct tttttaacta ctctgaaaaa tagcttaatg
    27421 gtcattgacc ctttaaataa tcctcttcct atgctcattt tctctttagg aataggtatt
    27481 ttccagattt taataggtat aatgctttct acgattaaag agtggaaaag aggtcatcgt
    27541 gctgtggctg ttatggacca tctgagctgg tttgtgtttt taacttctat agtcctgtac
    27601 atagtaggta ttgctgctct tccccttgta gcctcagtgg ctttaccggt ggtaataggt
    27661 agtgctattt ttttagtagc tactcaagga aggcatcaga aaaatcccat tatgaagttt
    27721 ttatctggtc ttatgagtct ttatggtttg gtttcatatc tgggggatac tctttcctat
    27781 tctcgtcttt ttgctttagg tctttctagc accattattg ccattttggc tcgtacttta
    27841 gggtctttgt ttggaggtta cccttatatt ggatggctca tcgccttgct ggtagcttta
    27901 gcctttcacg ttttcaattt gttgatgagt ggattgggtg cttttgttca ctctgctcgt
    27961 ttacagtatg tagagttctt taataagttt tatgaagatg ggggcaggaa ttttaaacct
    28021 tttggttata aaaccaaata tgtaaaaata agctgaagga ggttttgtaa tctatgattg
    28081 acggactggc attcgcactt ttaggttctg ctttagctat aggattggca ggaattggtt
    28141 cagcactggg agtaggcatc gctggagaag cgggcgcagg agtgttgacc gaagacccca
    28201 acaaatttgg gcaggttttg cttatgcaag cactacctgg aactcaaggt atttatggat
    28261 tgttggctgg attctgggta ttgattaagc tgggtctttt tggtggaact ccagtagaga
    28321 taactgttgc tcaaggttta cagattttat ttgcctgtct tcctatagca attgttggtc
    28381 ttctctccgg tatttctcag ggtaaaactt cggctgcttc tattgggatg attgctcgcc
    28441 gaccagaaga aacaggaaga gctattattc tccctgctat ggtagaaact tacgctgtct
    28501 tgtctttgct tgctaccatt cttcttttga attctattca agtttagtag aggcgcgaga
    28561 tgcctttaga agatattttg aaaaaaattg aaaaagaagc cgagcttaaa aaaaagagaa
    28621 ttttggaaga agctcgagct aaagccaatg ctcagttgga gagagctcaa agagaaatag
    28681 aaaaagaagc cgagcagttg ttatctaacc ataaaagaga aatagagctg gaagtgcaac
    28741 gcaagatttc tgaagcaaga ctggcaggta aaaatgaagt aggtaaagta aaatatgaaa
    28801 tctgggaaag attgcgccag gagctgatag aggcttttat caaaaacgta gaagcccaag
    28861 aagaagagtg gtgtaagaac tttctcagtg aacaaataat taatggtgat gaagaagtat
    28921 ggatggctcc tcgagaggta gagattttag gcagtaattt tttggaaaag ttgaaccagg
    28981 agaaaggttt gtctctgcgt ttcgggggag tggcagaaga cttagagcgg ggctttctct
    29041 taaaacgagg aggtatggtg gtggatctga gttttatttc tgcggtagag gactatatca
    29101 ggaagaatga agggagaatt tccgctctcc tttttgaggg ttaaccaatg agtgtagatt
    29161 tgagaaagcc tgctagtagc agcggagata ctcgctatac ttatttagta ggtagagtga
    29221 gagagctaga gcgaggactt ttgtctcaga gaacgttaga aactttactt cgctctgaga
    29281 atttagaaca agctctgcgg ggagtggcag agatccccta ttggggagaa gctttccaaa
    29341 atgttccttc ccgtacttat gatattgata gagttttggt gacccactac tggcaaaata
    29401 ttcaggaaat aaacagccat tccccgggga ataaaatagc tcgttttttt atctggcctt
    29461 ttgattttag tgagatgaaa ttaattttaa agcaccgttt agtgggtaaa gctctggaag
    29521 atacttaccc tactacttta gatgtggtaa gagtaaacag gtttttagga ggagaaactc
    29581 gagaatacct ctccgatctg tggattaaag ctataactga tagtttagaa gcttacgaaa
    29641 attaccgggt tattcaggta gtggaaatag ttttagaccg gcattatttg gagcaggttt
    29701 atctcttggc tcaggagatg gagagtaaag ttctcaagaa ctggctgtta gcttttgtgc
    29761 ttttggccta tttccgagca gtttttcgag ctcgttatca gcaaagaaaa ggggagttag
    29821 tgcgccttct tttctttgct cagccccttt tcccggaagg gaaactggta gaaatgttga
    29881 atgcttctcc agagaaagtg gcagaagagg taggtaattt tggttttgat ttccttcttc
    29941 ccgaaaaggg ggtttttcga gatgatcctt cttatttagc agaaatagaa aaaaatatgg
    30001 acaattatct tttagatttt atccgctctt atcggtggga agcctttggt ccagagccag
    30061 tttttgggtt tctctgggct caaggtatag acgttaagaa tttgcggttg attctggaag
    30121 gaaagtattt tggtttagaa aatgaaaa
//
LOCUS       DAOSVE010000012        29173 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_1068_length_29173_cov_67.632495, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000012 DAOSVE010000000
VERSION     DAOSVE010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 29173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29173
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1068_length_29173_cov_67.632495"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PK016_01970"
     CDS             <1..591
                     /locus_tag="PK016_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXL01877.1"
                     /translation="GGRKHPHQEFIQINTENILFICGGAFVGLDKIVESRLKDRKIGF
                     GAREMSSVPADSFNPLSEVQPEDLVRYGIIPEFVGRIPVICALDSLGEEDLVRILIEP
                     RNSLVKQYQSLLKMEGVELIFTEGALRLIAQEAMAKGTGARGLRAIMEKLMVELMFEL
                     PSRKDIEKVVINEEAVKTGTLLPFLERENTKEEQTA"
     gene            604..3237
                     /locus_tag="PK016_01975"
     CDS             604..3237
                     /locus_tag="PK016_01975"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HXL01878.1"
                     /translation="MNERTTLSSVFEPSSVEDKWYEFWEEKGYFTPPPSPAPPFSIVI
                     PPPNVTGYLHMGHALNLTLQDILIRYRRMQGYRALWVPGTDHAGIATQNVVEKQLSQE
                     GLSRHDLGREKFLENVWEWKERYHERIVKQLKKIGASCDWTRERFTLDEGLSRAVREV
                     FVRLFEEGLIYKDEYIVNWCPRCETALADIEVEYAEVSAHLYYVRYYLKDEDNYLVVA
                     TTRPETMLGDVALAVNPRDERYQELVGKVAILPLVGRELPIIADEYVDIEFGTGVLKV
                     TPAHDPHDFELGRKYGLPIAKVIDERGFMNEKALYFSGLSREEAREKVVEKLKEGGYL
                     EKIENYSHSVGHCYRCSTVVEPLVSKQWFVRVADLAQPAIKVVKEKKIEFIPERWEKV
                     YFEWMENIKDWCISRQLWWGHRIPVFYCQSCGYVFAHRNTPENCPHCGGEVKQDEDVL
                     DTWFSSSLWPFSVFGWPEDTEDLRTFYPTSVLITGFDIIFFWVARMIMMGLKFMGDVP
                     FRKVYITPLVRDAQGKKMSKSSGNAIDPLEIIDQYGADALRFALAWLTIQGRDINFSQ
                     ERIEASRNFMNKLWNVARFVLMNEEDFSVCEGLIDLDVKDRWIISRFHRLLGEVKEAL
                     DSLDFGRYAQLLYDFVWGEYCDWYIEWSKVDLYRGGEREKRRAQNILMYLLSGILKLL
                     HPVVPFITEEIWQSLPVREKESIMIASWPYQEEKWLDEEAEKTISFLQDVIREIRYLR
                     AELSIPPKEGCQVIFNFSDEGSYRYIEGNWSYVESLAKCSILEAGYNLKKPGSVATGR
                     VPGVDIYLLVGNMVDLNKEVERLNKKLSRLQTEEKRLKERLNDLSFLHRAPEEVVERE
                     KKRFADLIKEKEKLETLLEGI"
     gene            3237..3827
                     /locus_tag="PK016_01980"
     CDS             3237..3827
                     /locus_tag="PK016_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HXL01879.1"
                     /translation="MSNPFAFLKRQDPVETIVILLGGLVVVVIAFFIARGDLDYYTEY
                     LLSGGVFEREIEIKEVKVSPSPYFPLEEFLSQELSQELSSFPVPAEEEASPSPVVVTP
                     VPREKEVALSQKAETPPRAVEALSGEFFVQCGAFSRPENAQKMVEVLKGFGYQAVSES
                     VSGLYRVRIYGFQSRDEAEKAVAKLKGQGIESFVGK"
     gene            3838..4188
                     /locus_tag="PK016_01985"
     CDS             3838..4188
                     /locus_tag="PK016_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HXL01880.1"
                     /translation="MTLREIKEILQAEVLAGEEFLDREPSSACGSDLLSDVLAFTKEN
                     SVLLTGLTNSQVIRTAEMSDIIGVIFVRGKRPDNYTTELATIKKIPLLATRLPMYESC
                     GRLYLRGLRGCSEV"
     gene            4189..4632
                     /locus_tag="PK016_01990"
     CDS             4189..4632
                     /locus_tag="PK016_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXL01881.1"
                     /translation="MREDKVLVEIEREIVGGDFQSAGDVSSELKKVLQQVGFPSSFNR
                     RIVIAAYEAEMNVVIHAYRGIFTTQIYEDRVHIEVDDQGPGIPDLELAFQEGYSTAPP
                     HIREMGFGAGYGLSNMKKCSDRLEIDTRVGERTKVTMDFFLNSEK"
     gene            4639..5988
                     /locus_tag="PK016_01995"
     CDS             4639..5988
                     /locus_tag="PK016_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003871510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HXL01882.1"
                     /translation="MKEEIFHSVRLDEEKCKGCTHCIRHCPTEAIRVKEGKARIDEER
                     CIDCGECIRICPNHAKYAQSDSLEVIKRFPYAIALPAPAFYAQFKVDISLGKILQGLL
                     HLGFQEVFEVARGAEILAEEINHHLRKREIATPVISSACPAVVRLIEVRFPSLIENLL
                     PLDSPLGVAAKLARIQAMKKGYRPEEIGVFFITPCPAKVTESKQSENGLGRIDGAISI
                     ANIYSRLVNIVGELEESPSLPLASFRGIGWARSGGEQESLKEGEYMAVDGIHQVISVL
                     EKIEMGELKNVVYCEAQACVGGCIGGVLAVQNPFIAKVTINHLLKKYTEFRPLKKEEM
                     EEWRGKRIFERKEIYQAKEVLKLDKDFKRALEKYQIIEKLLEKLPGLDCGACGSPTCR
                     ALAEDIVRERAEEIDCPFMLRDRLLNLSEEIHALASKVPQTMAKKGGIKNLESESNH"
     gene            5972..6319
                     /locus_tag="PK016_02000"
     CDS             5972..6319
                     /locus_tag="PK016_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HXL01883.1"
                     /translation="MNQIIEPLKAEVVVGEKMEEQDIEGGYCGDLLSDCIANAKEESV
                     WVTIQSHPNIVAVAVLVGMPCIVVSNYQEIGEETVKRAQKEGITILRTSLTSYQAVSV
                     LSSLGIPGIRRHG"
     gene            6312..7028
                     /locus_tag="PK016_02005"
     CDS             6312..7028
                     /locus_tag="PK016_02005"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HXL01884.1"
                     /translation="MAKGVFLADLHIHSVLSPCADRDMLPHCIILEAREKNIDLIAIT
                     DHNACDNVEVTVALGEKFGIWVIPGLEVETREEIHLLSLFPSLSQLYDFNQEMEKCLP
                     FIPLQEKVWGEEWVINEEGEIKEKKTNLLVYPLDLSVEQVVEKTRSRGGIVIPAHVDR
                     PSYSIISQLGFIPPHLEIKVVELSINTSEEKATNKLGLAGFRFLHSSDAHSLTQIGSV
                     TTSFLFSSFPDWRELEQILT"
     gene            7142..7573
                     /gene="mraZ"
                     /locus_tag="PK016_02010"
     CDS             7142..7573
                     /gene="mraZ"
                     /locus_tag="PK016_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HXL01885.1"
                     /translation="MFLGHYYHAIDEKNRVVIPSSFRGEISGKVYLTQGMERCIFLYS
                     EEVWNRFYQKIATLSLTRKETREFSRFFLANATCLDIDGQGRIILPDYLKNYAEINRQ
                     VVWVGVGERLEIWAEEIWRPYHEEMGKKFGELTEQIVDTGI"
     gene            7576..8472
                     /gene="rsmH"
                     /locus_tag="PK016_02015"
     CDS             7576..8472
                     /gene="rsmH"
                     /locus_tag="PK016_02015"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HXL01886.1"
                     /translation="MNTYVFHQPVMEKEVLYYLLGRKDGIYVDATVGGGGHALSILGE
                     LEEGAMLVGIDRDEEAIKMAKETLKNPSLPVYLFKERFSIIDRILFSLGIEKISGILF
                     DLGVSSWQLDKAGRGFSFQKEGFLDMRMDLSEERNAYQVVNEFPEKELADLIYYYGEE
                     RFSRRIARAIVKERERKPIATTQELARIIEGVVPQRGKIHPATRTFMALRIFVNEELK
                     EIEEALKKVSPLVEEGGRVVVISYHSLEDRIAKRFFKNGEEWEILTKKPVYPTPEEVK
                     DNPRARSGRLRAGEKRSKIKEG"
     gene            8476..8808
                     /locus_tag="PK016_02020"
     CDS             8476..8808
                     /locus_tag="PK016_02020"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HXL01887.1"
                     /translation="MKRVKSWLIFGLFFIFSLSFLCVTLQALLLEKSFLVEQKREDIE
                     NLLATKEKLKMEIAQLSSLERIKEVATNQLGMTYPDKTVYMVSSSTLGGGEGELALNK
                     ILKETQGE"
     gene            8805..10496
                     /locus_tag="PK016_02025"
     CDS             8805..10496
                     /locus_tag="PK016_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HXL01888.1"
                     /translation="MISRKFARRANFLFLLSLFLLGIVVVRVFSLQVLQGKFYREEVL
                     SRLVLEEILPSRGSILDRSGKTLAEDVRVLSISAYPPQMDDPEQIAEELSQLLSLSAL
                     ELQQKFQSGRSWIEITDNAPLYLKETLEEKGLKGINWKEKPTRFYPQAPLFSNLLGFV
                     GKDRRGLEGLEFAFDSYLTGKLGYSFYEKDALGREIPLTTKYLSSQSGNNLHLTVDST
                     IQFFAEEALDRAMERTKALRGVVIVNNPRNGDILALASRPSFDNRNFQDFSPESWKNL
                     AISMVFEPGSTVKPLVMAATLEEKKASLEERFSCPGFIMVYNHRVRDIKAHGEEKLED
                     LLINSCNVGIITLAERLGKDKLFYYLKSFGLGERCEIGLPGEEAGLLRPPRDWSLLSM
                     GAIPIGQEMMVTPLQLLRALSSLANKGVVMQPRLVSEITSPTGEVIESFSPTPLGEIV
                     SERTASLVLEMMEKAAKEGTGKKAYIEGHRIAGKTGTGQKVGSGGSYDPHKFYSSFVG
                     FFPLPEPRFGVIVVLDEPQGEYYGGDIAAPVFREIAQNIINYASIVSDKAEVELF"
     gene            10493..12022
                     /locus_tag="PK016_02030"
     CDS             10493..12022
                     /locus_tag="PK016_02030"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HXL01889.1"
                     /translation="MTVSQFLSSFSFEEGRIENPEEEVRGLAFDSRRVRPGYVFFALP
                     GSNTDGHFYIGEAIKRGAVLIVLEKKEFAPPLQVKVSWVSVSEGRRALALASSFLYGD
                     PSSSLKIVGVTGTNGKTTTCFFLRTIWEKSGFPCGLLTTARNIIGPKEEEAINTTEES
                     LSLYHHLYEMRKMGIKNAVVEVSSHALALGRVEGVKFDSALITNLIPEHMEFHRTSEH
                     YFESKRKLLRMVEGNLSKSYPRVVAVNGDDENCLKMIKGIGVPFLTYGLNKEAEVRGE
                     DINYSLQQSSFSVKSPRGKMKVVIHFPGIHNVYNALAAISLALFYGLDINNIQEALFQ
                     VHQVPGRWEFVNAGQDFTIIVDFAHNWHGLEKSLSTIRDLTPGKVITVFGCGGERDRS
                     KRPFIGETVARFSDVCIITTDNPRGENPEQTAFDALEGVKRVTKNKNLHFEVILDRVE
                     AIEQALKLAQKGDTVFLAGKGPERFQVCNGKMIPHNDHYIAWKLLKENEILSSRNSRN
                     H"
     gene            11985..13397
                     /gene="murF"
                     /locus_tag="PK016_02035"
     CDS             11985..13397
                     /gene="murF"
                     /locus_tag="PK016_02035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HXL01890.1"
                     /translation="MKFYPQEIVEITEAEIQKTGKAPFFEGVAIDSRSLNAGQLFVAL
                     KGERTDGHLFVGEAIKRGAPGILVSHFDTSDTIEEETYVFQVPDTLLALQHLGRGASR
                     KLSGYKIAITGTAGKTTCKSFFASLLSKRFAVEATPQSFNTVIGVASSLANFKEDNDY
                     LIIEAGINRKKEMEELAEMINPRIVVFTSFGEGHLEGLGGVEEVVEEKFKLVREKTEI
                     IYLNTDQKWSRLVKEKKEIEDKRIISFGFSPVNHIYLKSFLLDPLTWKAKVEIKMGEN
                     NFCFFVPVLFPEIAIMLLPAFYLAYELGIKVEEIEESLRDWQPPEGRGNFFFHQGGII
                     IDDSYNANPLSSRKALRFLNSLNPFGFEAWGVWGDMLELGDTTSQAHQEFLRELKKSS
                     LSRALLVGKEMRKAAEEVAEEEIQEGRFLLFSNSLEVREFLFRHLPGRERWAILFKGS
                     RKLELEKAIPSEWRENRERD"
     gene            13384..14346
                     /gene="mraY"
                     /locus_tag="PK016_02040"
     CDS             13384..14346
                     /gene="mraY"
                     /locus_tag="PK016_02040"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HXL01891.1"
                     /translation="MNEIKSLIFSLLAVFALGLFSLPFFIRWQEKAHIGQKIKREGPN
                     LHLHKENTPSMGGIIIILSGLVVLCFFQRFTSPLWIILITLLSFGGLGLLDDYFKSFC
                     GRPWSLKARHKFLLELVFSVFILILASPFFPHYLIVPWGDGIIKLGEVPFFLYGLFLI
                     LASTNAFNITDGLDGLAAGCGILTFGFWTVLFLLEGNFSFAQVAIIFSGALLSFLWFN
                     FWPARIFMGDCGSLSLGALMGVLALFSGYSWLLALAGFVYLLDTLSVILQVFSFKVFG
                     RRIFLMSPLHHHFELRGEKESKITVKFWIIQGLGVVAALLGMMR"
     gene            14351..15694
                     /gene="murD"
                     /locus_tag="PK016_02045"
     CDS             14351..15694
                     /gene="murD"
                     /locus_tag="PK016_02045"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HXL01892.1"
                     /translation="MQVVLGLGKTGIAVSRFLLKQKEKVLAVDDYIKEEQIPRDIRES
                     EGFEFLSPSDFLKTDLSSISEVITSPGIPPAHPVLQILEERRISVISEIELATRFISF
                     PLIGITGSCGKSTTVFWTGQILEKAGWSVYVGGNWGCPLIDSLSAHEKFDWGVIELSS
                     FQLSRIKKARFSVAAMLNLFPNHLDYHSTIEDYFQAKARIFENQKKDDFAIINFSLSD
                     WSTRFSRLIRSQLVPVARDKKLNEGFYILGDKVFQERKELFSLSHFSLLGKHQRENLL
                     IAISIALLLGVKEQAVEEAISSLSSLPHRLEKIGSWEGIDYFNDSKSTTPSSTRVAIE
                     SLSCPIVLILGGRTKIDDLSELSSALRSGKVKAVVIYGESRNLIEKFIPSHLERYLVF
                     SLEEAVKKAKTLAKEGDAILLSPACTSWDQYHNFEERGEHFRQLVYELEQNKNSH"
     gene            15663..16814
                     /gene="ftsW"
                     /locus_tag="PK016_02050"
     CDS             15663..16814
                     /gene="ftsW"
                     /locus_tag="PK016_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HXL01893.1"
                     /translation="MNWNKIKIPINSEELSFKTRWWDIDPYLFWSMLLLLLLGIIMVF
                     SSSMTTALYAYGDVFYFLKRHLLGILLGFLFLTVFTFISLDRLRSINRFLLLATFVSL
                     ILVFLPGIGRARGGSYRWINLGPFGPQPSELAELSIILYLADALVNYKERVEDFWRGI
                     IPFMILVGIICLLVLIEPDLSTAFFLFLIAFIMLFLGGSRLQHLLLILIVLIPSGVFL
                     TLFGGKEYWQERIFAFIDPWKDPLGKGFHIIQSLIALGSGGITGVGLGESRQKFFFLP
                     DRHTDFIFAIIGEELGFLGTSAVVILFIIILWRGWKISQESQDEFRGLVAMGVTLSII
                     LQAFINMGAVLKLLPVTGVTLPLVSYGNSSLIITLSELGILFNIARGTK"
     gene            16815..17918
                     /gene="murG"
                     /locus_tag="PK016_02055"
     CDS             16815..17918
                     /gene="murG"
                     /locus_tag="PK016_02055"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HXL01894.1"
                     /translation="MGTIFIAAGGTGGHIFPSLCIAQEMKKRDLQVIFLGTKRGMEEK
                     LVGEAGFPLLFVRARGWNRKWDFSFFSMLGDNLVGFFQTAFYFFRYRPRALLGMGSYL
                     SLLSAVWARLLGVPVYIHEQNIYPGLSNQLVSRWAKKVFVSAPESITYWKDREEKIEV
                     VGNPLREEVISWKGKREEARQELGLEGKRQTILVMGGSRGSEIINTSFLEIVGWLEKK
                     GLQVIHITGEEGFERTKEMVKKFPFPYLTYKFLPQPGIAYAAADLAITRAGANTIFEL
                     LWFELPSVVIPYGEATDNHQLYNARWLAKQGLARVIEEKNLTSSLLIEEMEKILNSSS
                     CVFCDLKGKYLKDSAFRIVQTIIEELKRGKRLG"
     gene            17911..19296
                     /gene="murC"
                     /locus_tag="PK016_02060"
     CDS             17911..19296
                     /gene="murC"
                     /locus_tag="PK016_02060"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HXL01895.1"
                     /translation="MDKAHYLLPRNLHFIGIGGAGMSSLASIAHEQGYVVSGSDITEN
                     EAIARLKRKGIRVFLGHRMEQVWGAEAVIVSSAIPEENEELKEARRLNLPIIPRGEML
                     ASLVNSKKGIVVAGTHGKTTTTSMISLLLEVAGWDPTVSIGGELEDIGGNAKAGEGEF
                     FIAEADESDGSFLKLNPFCAVVTNIEDDHLIFYGDLEKEKEAFIRFLHQIKKEGFGVV
                     CQDHPAIRSILPYLSSLEILTYGMEEGDIRGIIKEKKKDGFLFQVNWKDKKLADFELR
                     IPGLHNINNALAAITVGLKLGIELNYLKKALFSFRGVKRRFEKVGRVKGALVIDDYAH
                     HPTEMLSVWQTALDYARGNLVVIFQPHRYTRTRQLYREMAKVLEKFQQVFILPVYSAG
                     EKPIPGVSAELIYQEMKKDGFEKVQLVESSEDAAEECLKILKPEDLVVTMGAGDVWKA
                     GWILCSQNGGK"
     gene            19272..20183
                     /gene="murB"
                     /locus_tag="PK016_02065"
     CDS             19272..20183
                     /gene="murB"
                     /locus_tag="PK016_02065"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HXL01896.1"
                     /translation="MLSKWRKVISQLGNIPYLSIVPQPEMKDFTTWKVGGKVLALVEV
                     KSGENLDQVISLCEEEGIEWRVLGRGSNILVKDEGFPGVVIKLGGEFRELKREGGYIE
                     GGAGVPLPRLVNFALEECLGGMEFLVGVPGTVGGAVRINAGCFGREIGSIVEKVEVRK
                     KNGKREWLKRDDLCFSYRHSNLKKEKTVILKAVFGLFPENREVLEKRTRKFSCLRKER
                     QPLEWPSAGSVFLNPEGDSAARIIEEMGFKGLRLGRAQISSKHSNFIINLGGAKSREI
                     EYLINWVQKEIYINKGVWLEREVELWS"
     gene            20254..20889
                     /locus_tag="PK016_02070"
     CDS             20254..20889
                     /locus_tag="PK016_02070"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HXL01897.1"
                     /translation="MFSLVFQTGIFKVKRIEISGNQQLQEEELLNIVGVPLDSNILAL
                     RSWEVEKRLEELPQIKEAKVRKVFPYVLQIEIKERQPEAWVEIGGRAFCVDEEGVEIP
                     ASSSTDLVKIRLSGNQKNLLLESLNLIKSWKKEFELPLSAVVVENDRLFILQLQNGIF
                     IKCEGVNNLKKKSAILKPYLRDISVKYLDVGGFDLRAGDDLVIVPSEEGAF"
     gene            20886..22151
                     /gene="ftsA"
                     /locus_tag="PK016_02075"
     CDS             20886..22151
                     /gene="ftsA"
                     /locus_tag="PK016_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HXL01898.1"
                     /translation="MKSWRSLDSHSPILGSVDVGTSKICTLIGQKKGSAVEVLGVGLS
                     SSRGIRRGKIIDINKAAGAIRESFSLALEIARVVPDLVYAGIAGDGVSSLNLENEVFL
                     NKGGREISARDVDKALEGSKAGVNLEGKRILHLLPQEYIVDDQTGIADPAGMIGSRLR
                     AKVHLITVDENQFHNFSKSFQQAGLEVHRLVFQPLASAMAVLHPMEKEMGITLVDIGG
                     GTADIAIFYGGSMRYSGVVPLGGESITSDLAVGLRTSRDEAERIKLHHGCAFSRHIPE
                     DEVIEAKDLGLSSLQTIKRKYACEIIEARVKELFLLIRRVIKSSGFYPFLRGGVVLTG
                     GSALLEGIAELGSSILELPVRVGSPESKDHVGMIQTVSHPMFSTACGLLQFGLEEVTD
                     RRARKSLVLDLENQRSKVIDWFKDFFMLD"
     gene            22170..23216
                     /gene="ftsZ"
                     /locus_tag="PK016_02080"
     CDS             22170..23216
                     /gene="ftsZ"
                     /locus_tag="PK016_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HXL01899.1"
                     /translation="MRSNSQGLRNGKVNIKVIGVGGGGGNAVNRMVQAGLKGVTFVAM
                     NTDTQVLERSLANVKLQIGAKLTRGLGAGANPEVGRKAAEEDKEKIFEILEGVDMLFV
                     TAGMGGGTGTGASPVVAGLAKELGILTVAVVTKPFTFEGKKRMSQAEEGINLLRERVD
                     SLIVIPNDRLLQVASPSTSVKEAFSMVDNVLLQGVKSISDLINIPGIVNLDFADVKAI
                     MEGAGTSLMGIGKAGGENRAREAAHLAVNSPLLETPFSGAKGVLFNVTGGPNLSIWDV
                     NQVAEVITKSASEEANIIFGAIIDEKMKDEVEITIIATGFDQSKEEKEEKVSEISDSF
                     SFDDLDVPAFLRKK"
     gene            23221..24780
                     /locus_tag="PK016_02085"
     CDS             23221..24780
                     /locus_tag="PK016_02085"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXL01900.1"
                     /translation="MVESAKDYWKEEQGISPSSKVGKFLFALCFPGDYSLGMTNLGYQ
                     SIIHLLFEAPDWRGERFFYEYGSRSLESNLPLNSFDIIGFNFSFELDILNLLLLLQEG
                     GIPLFARDRKEKDPWVISGGPAATLNPEIIAPFMDLIVIGEAEKVFPLILALWKEGEE
                     KGESREEKKRILGKIPGVYIPSKIHPVYQQGELRGFEEEGFSFPVKRQWVNINEFETR
                     TFIYTPFSYFQDASLVEISRGCSYRCSFCAGRSIYHPLRQRSLELVEKMIDHVSPWSS
                     KIGLVGADLLSHPALEEIIKYVLDKGKKLTLSSLSALSLVKKTGVRELLSKGEIETIT
                     LAPESGSFSARMKLGKGLSDEEWVDLVRELLEKKIKRVKLYFMLGKPEGKVEEDLEFL
                     AKMVAQIKNPQQISVSYSFLVPKPHTALSDWRTPSIYAWEREKRIWERGLGKLGIPFS
                     GESPRLAWIQLLLARGDRLIGEKIPQLLSHPHPWSWRTWQSLLQGLGRDEEWVRSPWK
                     EVKPWQVVDMG"
     gene            24723..25460
                     /locus_tag="PK016_02090"
     CDS             24723..25460
                     /locus_tag="PK016_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HXL01901.1"
                     /translation="MGTFTLERGKALAGGRYGVVENWEAVKEKIAIFSQRGGRNPEDI
                     KVVAVTKGVEVSKIEDAISLGLVSLGENRVQEALKKMPLLPRGIKWHFLGRVQRNKVK
                     KVVEFFQYLHSLDSIDRGEELNNRARDLSKKFPVLIQVNLTGRETQGGVKEKELLSLV
                     YQVVKLSHLELRGLMTIGPLGGEEQEVRKVFSRLRELKEEVNREMPQITNFQELSMGM
                     SDDFPWAILEGATILRLGRAIFGERGK"
     gene            25462..26268
                     /gene="proC"
                     /locus_tag="PK016_02095"
     CDS             25462..26268
                     /gene="proC"
                     /locus_tag="PK016_02095"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HXL01902.1"
                     /translation="MEDSILFVGCGNMGSALIQGLCRDNWHKKFRFFLLDQVAEKARS
                     LASSLGLEWVDSLNNLETPRFVFLAVKPKDVKDALSSLQNFSQSVFISVAAGVKIKDL
                     EKFLPQQAVVRIMPNLCVEVGEGVVPVCFSPAVKAEEREDLLFLFSSLGWVFEAEEED
                     LDLFTALSGSGPGFIALFIESLADGGVKIGIPWEKSLKIATQTVLGVASLLKTRGMHP
                     AQLKNWVASPGGTTIAGIKKLEEGAFRGLVMEGVEEAFKRAKNIGEEERK"
     gene            26265..>26721
                     /locus_tag="PK016_02100"
     CDS             26265..>26721
                     /locus_tag="PK016_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HXL01903.1"
                     /translation="MNWWKPDDVREIDFNRSFRGYNENEVQEFLEELASQIESMVAER
                     EKILQDNRELKRRLGEKEEKLSKMEAQMEEWEKQAEVERELAKRESQMLLKEAEVRAQ
                     KVVEEALERKKEIEKGYQDLLEQYRLFQIRFRSLLQTFMDSLEWKEKDWD"
     assembly_gap    26722..26821
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <26822..27207
                     /locus_tag="PK016_02105"
     CDS             <26822..27207
                     /locus_tag="PK016_02105"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HXL01904.1"
                     /translation="KQWAVESLTEGTIVLIPGILTLTLRHNFGSAFGWKIMSKEGLTL
                     ITVFITIILLWIFIKVVYIRGISLFLAGAWGNLLDRWRFGYVIDFIEPSFWATFNLAD
                     VFIVVGIILILLSFWFDEKREDSSL"
     gene            27179..28090
                     /locus_tag="PK016_02110"
     CDS             27179..28090
                     /locus_tag="PK016_02110"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HXL01905.1"
                     /translation="MRREKIQVYEPAFCRIDIWLHNHFPEVSRSLIIKLVREGKIKLN
                     GETITKASKEVYPGDIIEVNWPVEDISHPPPQLLPLDVIYEDEDILVVNKRAGFAVHP
                     TTSWGGGTLVNALFYYQIPLSQYGFPLRPGIVHRLDKNTSGVMVIAKSDKAYLHLIEE
                     FKARQVKKEYLALVEGIWEGEKTINLPLGRNIYNPCRMEVRKKDGKVAHTEIEVVGKG
                     KEFSLLLVKPKTGRTHQIRIHLASQGYPIVGDTVYGSKKGKEFFPRQALHAFTLSFYH
                     PSSREWVSFAASLPQDLRKFISDFIKI"
     gene            28105..28899
                     /locus_tag="PK016_02115"
     CDS             28105..28899
                     /locus_tag="PK016_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004543221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL01906.1"
                     /translation="MDLGLKNKIAVITGGSVGIGYAVAQAFAHEGVHLALCARGEERV
                     KEAAHTLQDKYGIRAIGVKADVSKVEDINNFVKEIEKEWEVVDILVNNAGTGSAEKIM
                     EAEDEKWQFYWDLHVMAAVRMSRALIPLMKKRGGGVIINNASICARQPLDYEPIYNVT
                     KAALAMFSKCLANEVIKDNIRVNSINPGLIRTPDWDKTANIVSKEKGITPEEYLDKIA
                     KDNAPIGRFATPEELANFFVFLASDCASYCVGSTYYVDGGWLKVVI"
BASE COUNT         8534 a         4668 c         7119 g         8752 t
ORIGIN      
        1 ggaggaagga aacaccccca tcaggaattt atccagatta ataccgaaaa cattcttttt
       61 atttgtgggg gtgcttttgt aggtttagat aaaatagttg aaagtcgctt aaaagaccgc
      121 aagattggtt ttggagcacg agaaatgtct tccgttcctg cggattcgtt taatccttta
      181 agtgaggttc aaccggaaga cctggtgaga tacggtatta tccccgaatt tgtggggcgt
      241 attccagtga tttgtgctct tgactcctta ggagaagaag atctggtacg tattcttatt
      301 gaacctcgta atagcctggt caaacagtat cagagcctgt taaaaatgga aggagtggag
      361 ctcattttta ctgaaggtgc actacgtctt attgcccaag aggctatggc taaaggcaca
      421 ggagcacgag ggttgcgtgc cataatggaa aagctaatgg ttgagctgat gtttgagctg
      481 ccttctcgca aggatattga aaaagtggtg attaatgaag aagcagttaa aactgggact
      541 ctgcttcctt ttttagagcg agaaaacact aaagaagagc aaacggcgta aaggggaaaa
      601 agaatgaatg aacgaactac tctttcctcg gtttttgaac cttcttcggt ggaagataaa
      661 tggtatgaat tctgggagga gaaaggttac tttactcctc ctccctcccc ggctcctcct
      721 ttttctatag ttattcctcc tcctaacgtt actggctatc ttcatatggg gcatgcctta
      781 aatcttaccc ttcaggatat attaatccgt tatcgaagga tgcagggtta tcgggctctc
      841 tgggtgccag gaactgacca tgctggcatt gctactcaaa atgtggtgga aaagcaatta
      901 tcccaggaag ggctaagtcg ccatgattta ggtagagaaa agttcctgga aaacgtctgg
      961 gaatggaaag aacgctatca tgagagaatt gtaaaacaat tgaaaaagat aggagcttct
     1021 tgtgattgga cgagagagcg ttttacttta gatgaaggtt tatcccgggc agtacgggaa
     1081 gtttttgtcc ggctttttga agagggcttg atttataaag atgagtacat tgtaaactgg
     1141 tgtccccgtt gtgaaacagc actggctgat attgaggtag agtatgctga ggtttctgct
     1201 catctttatt atgtgagata ctatctcaaa gatgaagata attacttggt ggtagctact
     1261 acaagaccgg agaccatgct tggagatgta gctttagcgg taaaccctcg tgatgagcgc
     1321 tatcaggaac tggtagggaa ggttgctatt ttaccgcttg taggtagaga acttcccatt
     1381 attgctgatg aatatgtgga tatcgaattt gggactggag tgcttaaggt tactcctgct
     1441 catgaccccc atgattttga actgggaaga aaatatggat tacctatagc caaagttatt
     1501 gatgagcggg gctttatgaa tgaaaaagct ctatattttt ctggtctttc tcgagaagag
     1561 gcccgggaaa aagtggtaga aaagttaaaa gaggggggat atttagaaaa aatagaaaat
     1621 tattctcatt cagtggggca ctgttaccgt tgctctactg tggtagagcc tctggtttct
     1681 aaacaatggt ttgttcgagt agcggacctg gctcagccgg ctataaaggt agttaaagaa
     1741 aaaaaaatag agtttatccc tgagcgctgg gaaaaagtct attttgagtg gatggagaat
     1801 atcaaagatt ggtgtatttc tcgtcaattg tggtggggac atcggattcc tgtcttttat
     1861 tgtcagagtt gtggctatgt ttttgctcat cgaaatactc cagaaaattg tccccactgt
     1921 ggcggagagg taaagcaaga tgaggatgtt ttagatacct ggttcagttc ctccctctgg
     1981 cctttttctg tgtttgggtg gccagaagat actgaagacc tgagaacttt ttatcccact
     2041 tcggttttga ttactggctt tgatataatt ttcttctggg tagcacgcat gatcatgatg
     2101 ggtttgaaat ttatgggaga tgttcccttc cgaaaagttt atattactcc tttggtgcgt
     2161 gatgctcagg gaaagaaaat gagtaagtct tcaggtaatg ctattgaccc tttagaaata
     2221 atagaccaat atggtgcaga cgctttacgc tttgctcttg cctggcttac gattcaaggt
     2281 agagacatta acttttctca ggaacggata gaggcctcac gcaatttcat gaataaattg
     2341 tggaatgttg ctcgctttgt gttgatgaac gaagaggatt tttcagtttg tgaggggtta
     2401 atcgatcttg atgttaaaga ccgctggatt atttctcgtt ttcatcgttt actgggtgag
     2461 gtaaaagagg cactggatag cttagatttt ggccggtatg cccagcttct ttacgatttt
     2521 gtgtggggag aatattgtga ttggtatatt gaatggagta aggtagatct ttaccgagga
     2581 ggagagagag aaaaaagaag agctcaaaac atcctcatgt atctgctttc agggatttta
     2641 aaacttttac atcccgtggt tccgtttatc actgaagaga tctggcaaag tcttcctgtg
     2701 agggagaaag agagtattat gattgcttcc tggccttacc aagaagaaaa atggttagat
     2761 gaagaagcag agaaaactat ctccttttta caggatgtta taagagaaat tcgttattta
     2821 cgagcagaac tgagtattcc tcctaaagaa ggatgtcagg tcatatttaa tttctctgat
     2881 gagggaagtt accggtatat agagggtaac tggtcttatg tggaaagtct ggctaaatgt
     2941 tcgatactgg aggccggtta taatttgaaa aagcctggat cagtagctac gggaagagtg
     3001 ccaggagtgg acatttatct tttggtggga aatatggttg acttgaataa ggaggtggag
     3061 cgtttgaata agaagctgtc ccgtcttcaa acggaagaga aaaggttgaa agaacgcctt
     3121 aacgacctct cttttctgca tcgagctccg gaagaagtgg tagaaaggga aaaaaaacgt
     3181 tttgctgatt tgattaagga aaaagaaaaa ttagagactt tacttgaggg tatttgatga
     3241 gtaatccttt tgcttttttg aaaagacagg acccggtaga aacaattgta attcttctcg
     3301 ggggtttggt ggtagtagtt atcgcttttt ttatagcccg gggagattta gattattata
     3361 ctgaatacct tctcagtggt ggtgtctttg agagagaaat agagataaaa gaagtgaaag
     3421 tttctccctc tccttatttc cctctggagg agtttttgtc tcaagaattg tctcaggagt
     3481 taagttcttt tccagtacca gcagaagaag aagcttctcc ttctccggta gttgtaactc
     3541 ctgttccccg ggaaaaagaa gttgcacttt ctcaaaaagc tgaaacccct cctcgagcgg
     3601 ttgaggctct ttcgggagaa ttttttgttc aatgtggagc attttctcga ccagaaaacg
     3661 ctcaaaaaat ggtagaggtc ttgaaagggt ttggctatca agctgtttct gaatccgttt
     3721 caggattgta tcgagtgaga atatatggtt tccaaagtag agacgaagct gagaaagcag
     3781 ttgccaagtt aaagggtcag ggaatagaat cttttgtggg aaagtaggga aaattatatg
     3841 accttgcgag agataaaaga gatattgcag gccgaggtgt tagcaggaga agaattcctg
     3901 gatagagaac catcctctgc ttgcggtagc gaccttttaa gtgatgttct ggcttttacc
     3961 aaggaaaact ctgtgctcct taccggttta actaattctc aggtaatccg tactgcggaa
     4021 atgagtgaca taataggggt tatttttgtg cgaggtaaaa ggcccgataa ttataccact
     4081 gagctagcta ccattaagaa aattccactt ttagctaccc ggttacctat gtatgaatcc
     4141 tgcgggagac tttatttgcg gggactgaga ggttgtagtg aagtatgaat gagagaagat
     4201 aaggttttag tagaaataga gcgggaaatt gtgggaggag attttcaaag cgctggtgat
     4261 gtgtccagtg aattaaaaaa agttcttcag caggtaggtt tcccttcttc ttttaatcgt
     4321 cgaatagtaa ttgccgctta tgaagcagaa atgaatgtag taatccatgc ttaccggggg
     4381 atttttacca ctcaaatcta tgaggaccgg gtgcatattg aagtagatga ccagggtccg
     4441 ggtataccag atttagagct ggcctttcaa gaaggttatt ctactgctcc tcctcacata
     4501 agagagatgg gttttggagc gggatatggg ctttccaata tgaagaaatg ttctgatcgt
     4561 ctggaaattg atacccgggt aggagagaga actaaagtga ctatggattt ttttcttaac
     4621 tcggagaaat aggttatgat gaaggaggaa atttttcatt ctgtccgcct ggatgaagaa
     4681 aaatgcaaag gttgtaccca ttgtattcgc cactgtccca cggaggccat tcgagtaaaa
     4741 gaaggaaaag ctcgcattga cgaagagaga tgtattgatt gtggagaatg tatcaggatc
     4801 tgtcctaatc atgctaaata cgctcaatct gattctttag aagttataaa aagattccct
     4861 tacgccatag ctcttcctgc tcctgctttt tatgctcagt ttaaagtaga tatttcgctg
     4921 ggcaaaatac tccagggtct tttgcatctg ggctttcagg aagtttttga ggtggctcgg
     4981 ggagcagaaa ttctggcgga ggaaattaac catcatctga gaaagcgaga aatagctact
     5041 ccggtgattt cttctgcttg tcctgcagta gtaaggctga tagaggtcag gttcccttcc
     5101 ttaatagaaa atttgcttcc tctggattct cctctaggag tagcagcaaa gttagcaagg
     5161 atacaggcta tgaaaaaggg ataccgtccg gaagaaatag gagttttttt cattactcct
     5221 tgtccggcta aagtaactga atctaaacaa agcgaaaatg gtctgggacg tatagatgga
     5281 gctatctcta tagctaatat ttattctcgt ctcgttaata tagtagggga gctagaagaa
     5341 tctccttctt tacctctggc cagtttccgg ggtattggct gggctcgctc aggaggagaa
     5401 caggagtcat taaaagaagg agagtatatg gctgtagatg gtatccatca agttatttca
     5461 gtactggaga aaattgagat gggagaattg aaaaatgtgg tttactgtga ggctcaagcc
     5521 tgtgtaggag gatgtattgg aggagtattg gcagtgcaga atccttttat tgctaaggta
     5581 actattaatc atttgcttaa aaaatatacg gaatttcgac ccctaaaaaa ggaagaaatg
     5641 gaagaatggc gtggaaagag aatatttgaa aggaaggaga tttatcaggc taaagaagtt
     5701 ttaaaattgg ataaggattt caaacgagcc ctggaaaaat atcaaataat tgaaaagctt
     5761 ttagaaaaac ttcccggtct ggactgtgga gcctgtggtt ctcctacctg tagagctctg
     5821 gccgaagata tagtaaggga gagagcggaa gagatagatt gcccttttat gttacgagat
     5881 cggttactca atttatctga agaaattcat gctttagcca gcaaggttcc gcaaaccatg
     5941 gctaaaaaag gaggaataaa aaatcttgaa agtgaatcaa atcattgaac cgttgaaagc
     6001 ggaagtagta gtaggggaga aaatggagga gcaggatata gaaggggggt actgtggaga
     6061 ccttctcagt gattgtattg ccaacgctaa agaggaaagt gtctgggtta ccattcaatc
     6121 tcatcctaac atagtggcgg tggcagtact agtaggtatg ccctgtatag tggtttccaa
     6181 ttaccaggag ataggagagg aaacagtaaa gagagcccaa aaagagggta taactatatt
     6241 gcgcacttct ttaaccagct atcaggcagt tagtgttctt tcttctctgg gaataccagg
     6301 catcagaaga catggctaaa ggagtttttt tagctgacct ccacattcac agtgttttat
     6361 ctccttgtgc tgatcgggat atgttacctc actgtatcat tctggaagcc cgggaaaaaa
     6421 acattgattt gattgccata actgaccaca atgcctgtga taatgtagag gtaactgtgg
     6481 ccttgggaga aaagtttggt atctgggtga taccaggatt agaggtggaa accagggaag
     6541 aaatacatct tctctccctc tttccttctt tatcccagct ttacgatttt aatcaagaaa
     6601 tggaaaaatg tcttcctttt attcccttgc aagaaaaagt atggggggaa gagtgggtaa
     6661 taaatgagga aggagagata aaagaaaaaa agaccaatct cctggtttac cctttagatt
     6721 tatctgtaga acaagtggtg gaaaaaacaa gaagccgggg aggaatagtt attcctgctc
     6781 atgtagaccg tcccagctat agtattattt ctcaacttgg ttttatccct ccccatctgg
     6841 aaataaaagt ggtagagtta tcaatcaata cttctgaaga aaaagcaaca aataaattgg
     6901 gtttagcagg ttttcgattc ctccactctt ctgatgctca ctctcttact caaataggtt
     6961 cagtcactac ttctttttta ttctcttctt ttcctgactg gagagaactg gaacaaatcc
     7021 tcacctgaag tttttcttcg gtaaaaaatc cttttttaaa acgaacgttc tatttatctt
     7081 tcctgtttat tagtttatgc taagtggtgt ataatggtaa aaagtggggg aaagtgggaa
     7141 aatgttttta ggccattatt accacgctat tgacgaaaag aaccgggtag taattcccag
     7201 ttcttttcgg ggagaaattt cgggaaaggt ttatcttact caaggaatgg aaagatgtat
     7261 ttttctctat tccgaagaag tgtggaacag gttttatcaa aaaattgcca ctctttctct
     7321 tactagaaag gaaactcgag agttttctcg cttctttcta gctaatgcta cctgtctcga
     7381 tatcgatgga caaggaagaa ttattctccc cgattacttg aaaaattatg ctgaaataaa
     7441 ccgccaggtg gtctgggtag gagtgggaga gcgattggaa atctgggctg aagaaatctg
     7501 gaggccctat catgaggaga tgggaaagaa gtttggtgag cttactgaac agatagtgga
     7561 tacgggtatt taaggatgaa cacttatgtt ttccatcagc cggtaatgga aaaagaagta
     7621 ctctattatc ttctcgggag gaaagacgga atttatgtag atgctacagt gggaggagga
     7681 ggccatgctc tatctatttt gggggaactt gaagaaggag ctatgttagt gggaatagac
     7741 cgagatgaag aagctataaa aatggccaaa gaaaccctga agaatccttc ccttccagta
     7801 tatttattta aagaaaggtt ttctataatt gaccgaattc ttttttctct gggtatagaa
     7861 aaaatttcgg gaatattgtt tgacctgggg gtgtcttcct ggcagttaga caaagcagga
     7921 agaggcttta gcttccagaa ggaaggattt ttggatatga gaatggattt atctgaagaa
     7981 cgaaatgctt accaggtggt caatgaattt ccggagaaag aattagccga tttgatttat
     8041 tattacgggg aagaaaggtt ttctcgaaga attgctcggg ccatagtaaa agagagagag
     8101 cgaaaaccca tagctactac ccaagaatta gcccgaatta tagaaggagt agttcctcaa
     8161 cgaggaaaaa ttcaccctgc tactcgtacc tttatggctc tgcgtatatt tgtgaatgaa
     8221 gaattaaagg aaatagaaga agctttgaaa aaagtttctc ctctagtaga ggagggggga
     8281 agagtggtgg tgattagcta tcattctctg gaggatcgga tagcaaaaag gttttttaaa
     8341 aatggagaag agtgggagat actgactaaa aaacctgtgt atcccactcc cgaagaagtg
     8401 aaagataatc ctcgagctcg tagtggccgt ttaagggccg gagaaaagcg aagtaaaatt
     8461 aaggagggat aagggatgaa gcgggttaaa tcctggttaa tatttggttt attttttatt
     8521 ttttctctct ctttcctctg cgttaccttg caggctttac ttttagagaa aagttttttg
     8581 gtggagcaaa aaagagagga tatcgagaat ttactggcaa ccaaggagaa attaaaaatg
     8641 gaaattgctc aactatcttc actggagaga ataaaagaag tagcaacaaa ccagctgggt
     8701 atgacctatc ccgataaaac tgtctatatg gtatctagct ctactctagg aggtggggaa
     8761 ggagagttgg ctctgaataa aatacttaag gagacacagg gagaatgatt tccagaaaat
     8821 ttgcccgccg ggcaaatttt ctgttccttt taagcttgtt ccttttagga attgttgtag
     8881 ttcgtgtctt ttcccttcag gtattacagg gtaagtttta tcgagaagaa gttttgtctc
     8941 gtttggtatt agaggaaatt ttgccttctc gagggtcaat tctggaccgc agtggtaaaa
     9001 cactggctga ggatgtaaga gtcctttcta ttagtgctta ccctcctcag atggatgacc
     9061 cggagcaaat agcggaagaa ttatctcagc ttctttccct ttctgcgctt gaattacaac
     9121 aaaagtttca gagtggacgt tcttggatag agataaccga caacgcacct ctttatttaa
     9181 aagagacttt agaggagaaa ggactgaaag ggataaactg gaaagaaaaa ccaactcgtt
     9241 tttaccctca agctccttta ttcagtaatc tcctgggctt tgtgggaaag gacaggcggg
     9301 ggttagaagg tttagaattt gccttcgatt cttatcttac tggtaaatta ggttattcat
     9361 tttacgaaaa agacgcgcta gggagagaaa taccccttac tactaaatat ttatcatctc
     9421 agagtggtaa caatctccat ttaactgtag atagtactat ccagtttttt gctgaagaag
     9481 ctctggaccg ggctatggaa aggactaaag ctcttcgagg agtagtaata gtaaataatc
     9541 cccggaatgg ggatatttta gctctggcca gtcgtccttc ttttgacaat cgcaactttc
     9601 aagatttttc tcctgaatcc tggaaaaacc tggccatcag tatggttttt gagccaggct
     9661 ctacggtgaa gccactggta atggctgcaa ctctggaaga aaaaaaagct tctcttgaag
     9721 agagattctc ttgtcctggt tttattatgg tgtataatca tcgggtgcga gatataaaag
     9781 ctcatggtga ggaaaaattg gaagatttgt taattaattc ctgtaatgtg ggaattatta
     9841 ctttagctga gagattgggt aaagataaac tattttatta tttgaagagt tttggattag
     9901 gagaaagatg tgagataggg ctaccggggg aagaagcagg tcttttacgt cctccccggg
     9961 attggtcctt actttctatg ggagccattc ctattgggca ggagatgatg gttactcccc
    10021 tacaactttt aagagcgctt tcttctctgg ctaataaggg ggtggtaatg caacctcgtc
    10081 tggtgagtga aattacctct cccacgggag aagtgataga aagtttttct cctactccct
    10141 taggggagat agtatcagag cggacagcct ctttagttct ggaaatgatg gaaaaggcag
    10201 cgaaagaagg gacaggaaag aaagcttata tagaagggca tcgtatagcg ggaaagactg
    10261 gaaccggtca aaaagtaggc tctggtggta gctatgatcc tcataagttt tattcttctt
    10321 ttgtgggttt tttccctctt cccgagcctc gctttggagt tattgtggtt ctggatgaac
    10381 cacaaggaga atattatggt ggagacattg ctgctcctgt ttttagagaa attgctcaaa
    10441 atatcattaa ctatgcttct atagtgtcag ataaagcgga ggtagagttg ttttgactgt
    10501 aagtcaattt ttatcttcgt tttcttttga agaaggaaga attgaaaacc cggaggaaga
    10561 agttagaggt cttgcttttg attctcgtcg ggtacggcct ggttatgttt tttttgccct
    10621 tcctggttct aacaccgatg gtcactttta tattggagaa gcaataaaaa gaggagcagt
    10681 tttaatagtt ttagaaaaaa aagaatttgc tccccctcta caggttaagg tgtcctgggt
    10741 ttcagtctct gaggggagac gagctctggc tttagcttct tcctttcttt atggtgatcc
    10801 ttcttcttcc ctcaagattg tgggagtaac aggcactaat ggaaagacca ctacttgttt
    10861 ttttctccgt actatctggg aaaaaagcgg atttccctgt ggtctcctta ctacagcgcg
    10921 taacattata ggtcccaaag aagaagaagc gataaacact actgaggagt ctctttctct
    10981 ttatcatcat ctttatgaga tgcgaaaaat gggtataaaa aacgcagtag tcgaagtttc
    11041 ctctcatgct ttagctttag gaagagtgga aggagtgaaa tttgattctg ccctaattac
    11101 caacttaatt cctgaacata tggagtttca ccgtacttcc gaacattatt ttgaaagtaa
    11161 aagaaaactc ctacggatgg tagaggggaa tctttctaag agttatcctc gggtggtagc
    11221 agtaaacggg gatgatgaaa attgccttaa gatgataaaa ggaataggag ttccttttct
    11281 tacttatggt ttgaacaaag aggcagaggt gaggggagaa gatattaatt actctcttca
    11341 acaaagttct ttctcagtaa agagtcctag ggggaaaatg aaggtggtaa ttcattttcc
    11401 cggtattcat aacgtgtaca atgctcttgc tgccatttct ctggcccttt tttatggttt
    11461 agacataaac aatattcaag aagcactatt tcaggttcat caagttcctg gccggtggga
    11521 gtttgtaaat gcaggccagg attttaccat catagtagat tttgctcata actggcatgg
    11581 attagaaaaa agcctctcta ctattcgaga tttaactccg ggaaaggtaa ttactgtatt
    11641 tggctgtggg ggagaaaggg acaggagtaa aaggcctttc attggagaaa cagtggctcg
    11701 cttcagtgac gtgtgtatta ttaccactga taatccccgg ggagaaaatc cggaacagac
    11761 tgcttttgat gctttagagg gagtaaaaag agtaacgaag aacaaaaatc tacattttga
    11821 agtaattctg gatcgggtgg aagctatcga gcaagctttg aaattggctc aaaaagggga
    11881 tacagttttt ctggctggaa aaggccctga gagattccag gtgtgtaatg ggaaaatgat
    11941 tcctcacaat gaccattata tagcctggaa attgttgaaa gaaaatgaaa ttttatcctc
    12001 aagaaatagt agaaatcact gaagctgaaa tacagaaaac agggaaagcc cccttttttg
    12061 agggggtggc tattgatagc cgttccctga acgcagggca gctttttgtg gcacttaaag
    12121 gcgaaaggac tgatgggcat ttatttgtgg gggaagcaat aaaaagaggc gctccaggaa
    12181 ttctggtcag tcattttgat acttctgata ctatagaaga ggaaacttat gtttttcaag
    12241 tgccggatac tcttttagct ctccagcatc ttggaagggg ggcttctcgg aagttatcgg
    12301 ggtataaaat tgccataacc ggcacagcag gcaaaactac ctgtaagagt ttttttgctt
    12361 ctctcttatc caagcgtttt gcagtagagg cgactcctca gagttttaac accgtaatag
    12421 gagtggcaag ctccttagct aattttaaag aagacaatga ttatttaata atagaagcag
    12481 ggataaacag aaaaaaggag atggaagaat tagcggaaat gattaaccct cgcatagtgg
    12541 tttttacttc tttcggggaa gggcacctgg agggtttagg tggtgtagaa gaagtagtag
    12601 aagaaaaatt taaactggtc agggaaaaaa ccgaaataat ttatcttaat accgaccaga
    12661 aatggagcag gttagtaaag gagaaaaaag aaatagaaga caaaagaatc atttcttttg
    12721 gcttttctcc tgtgaatcac atttatttaa aaagtttttt acttgaccct ctgacctgga
    12781 aagctaaagt agaaataaag atgggagaga ataacttttg tttttttgtt cctgttcttt
    12841 ttcctgaaat agcaattatg ttattacctg ctttttatct agcctatgag ctgggtataa
    12901 aggtggagga aatagaagag agtttgaggg actggcaacc cccggaggga agaggtaatt
    12961 ttttcttcca ccagggagga attatcatcg atgattctta taatgccaat cctctttctt
    13021 ctcgtaaggc tttacggttt ctgaattctt taaatccttt cggttttgaa gcctggggtg
    13081 tgtgggggga tatgttagaa ttaggagata ccacttctca agctcaccag gagtttttga
    13141 gagaactgaa aaagtcttct ctttctcggg ctctattagt gggaaaagaa atgaggaagg
    13201 cagctgaaga ggtggctgaa gaggaaatac aggaaggccg atttttactc ttttctaact
    13261 ccctggaggt gcgagaattt ctcttccgtc atcttccagg tagagaacgg tgggcaatac
    13321 tttttaaggg gtcgagaaaa ttagaactgg aaaaagctat tccttcagag tggagagaaa
    13381 atcgtgaacg agattaaaag cttaattttt tctcttctag cagtttttgc tctggggtta
    13441 ttttcccttc ctttttttat tcgctggcaa gaaaaagccc atataggtca gaaaataaaa
    13501 agggaaggtc ccaaccttca tcttcataag gaaaacactc ccagcatggg aggaataatc
    13561 attattctct ctgggttagt ggtactttgt ttttttcagc gatttacttc tccgctctgg
    13621 attatactga taactttgct atcttttggc ggattgggtt tattagatga ctactttaaa
    13681 agtttttgcg gaaggccctg gagcttaaaa gctcgccaca aatttttatt agaactggta
    13741 ttttctgttt ttattttaat tttggcgtct cccttttttc ctcactattt aattgttccc
    13801 tggggagatg gaataataaa attaggggaa gtgccttttt tcctttatgg cttattttta
    13861 atcctggcct ctactaatgc ttttaatata actgatggtc tggatggatt ggcagcaggt
    13921 tgtggaattt tgacttttgg cttctggaca gtgctttttt tgctggaggg aaatttttct
    13981 ttcgctcagg tagcaatcat ttttagcgga gcgctgcttt cttttctctg gtttaatttc
    14041 tggccggccc gaatttttat gggagattgt ggttctctgt ctttaggagc tcttatggga
    14101 gttttagctc ttttttccgg ttattcctgg ctattggctt tagctggttt tgtctatctt
    14161 ctggatactc tttcggtaat tcttcaagta ttttccttca aagtgtttgg aagaagaata
    14221 tttcttatga gtcctcttca tcaccatttt gagctccgag gagagaaaga aagtaaaatt
    14281 actgttaaat tctggatcat ccagggatta ggagtggtag ccgcactttt aggaatgatg
    14341 aggtgaaaaa atgcaggtag ttctgggtct gggtaaaaca ggaatagcag ttagccgatt
    14401 tctcctcaag cagaaagaga aagttttagc tgtagatgac tatattaaag aggagcagat
    14461 acctcgggat ataagagaaa gcgaaggatt tgagtttctt tctccgtccg actttttaaa
    14521 gactgacctt tcttctattt ctgaagtcat aaccagtcct ggtataccgc cagctcatcc
    14581 agttttacag attctggagg aacgtagaat atcagtgatt agcgagatag aattagctac
    14641 tcggtttatt tcctttcctc tcattggaat taccggttct tgtggaaaga gtaccactgt
    14701 tttctggacc ggacaaattt tagagaaagc tggctggtca gtttatgtgg gaggtaactg
    14761 gggctgtcct ttgatagata gtctttccgc tcacgaaaaa tttgattggg gagtaataga
    14821 gttgagtagc tttcagttgt ctcgcatcaa gaaagctcgt ttttcggtag cagcaatgtt
    14881 gaatttgttt cctaatcact tagattatca ttctactata gaagattatt ttcaagctaa
    14941 agctcgcatt tttgagaatc aaaaaaaaga tgactttgct atcatcaatt tttctctcag
    15001 tgactggtca actcgttttt ctcgtttgat tagatctcaa ttggtgccag tagctcgaga
    15061 taaaaaactt aacgagggat tttatatcct gggagataaa gtttttcaag agaggaaaga
    15121 gcttttttct ctttctcact tttctcttct cgggaaacat cagcgagaga atttactcat
    15181 tgctatatcc atagctttac ttttaggagt aaaggagcag gcagtggaag aggcaatatc
    15241 ttctctttct tctcttcctc atcgtttaga gaagatagga agctgggaag ggatagatta
    15301 ttttaatgat tccaaatcca ccactccttc ttcgaccaga gtggctatag agtctttatc
    15361 ttgccctatt gttttaatcc tgggaggaag aacaaaaatt gatgacctca gtgaacttag
    15421 ctctgctctc cggagtggta aagttaaagc agtggttatc tatggggaat ctcgaaatct
    15481 gatagaaaag tttattccct ctcatcttga acgatattta gttttttctc tggaagaagc
    15541 ggtaaagaaa gcaaaaactt tagctaaaga gggtgatgct attcttcttt ctccggcctg
    15601 taccagctgg gaccaatatc ataattttga ggaaagggga gagcacttcc gtcaattggt
    15661 ttatgaattg gaacaaaata aaaattccca ttaatagtga agaactttct tttaaaacca
    15721 ggtggtggga cattgatccc taccttttct ggtccatgct tttgctgcta cttttgggaa
    15781 ttatcatggt ttttagctcc agcatgacta ctgctttgta tgcttatggt gatgtttttt
    15841 actttttaaa aaggcatctt ttaggaattt tactgggatt tcttttttta actgtgttta
    15901 cttttatttc tttagaccgc ttacggagta taaatcgatt cttacttttg gctacttttg
    15961 tttctttgat tttagttttc ttacctggta taggtagagc tagagggggt tcttatcggt
    16021 ggataaattt ggggcctttc ggtcctcaac cttcagaatt agctgagttg agtataattt
    16081 tatacttagc cgatgctctg gtaaactaca aagaaagagt ggaagatttc tggcgaggga
    16141 ttataccttt tatgatttta gtgggaataa tctgtcttct agtattgatt gagccagatc
    16201 tgagtaccgc attctttcta tttcttattg cttttattat gctattttta ggaggtagca
    16261 ggttgcagca tctccttttg attttgatag ttctaatacc cagtggtgtt tttctcaccc
    16321 tttttggagg taaagaatac tggcaagagc ggatttttgc ttttattgat ccatggaaag
    16381 accccttagg aaaggggttt cacatcattc aatctcttat agccttaggt tcaggcggga
    16441 taacaggagt agggttaggt gaaagtcgcc aaaagttttt tttccttccc gatcgccaca
    16501 ctgatttcat ttttgctatc ataggagaag aattaggatt tctagggact tcagcggtgg
    16561 tgatactatt tatcattatc ttgtggcgag gttggaaaat ttctcaagaa tctcaagatg
    16621 aattccgagg acttgtggct atgggtgtta ccctcagtat tatattgcag gctttcatta
    16681 acatgggagc ggtgttgaaa ctgcttccag ttacgggagt gactttgcct ttagtaagct
    16741 atggtaactc ttctttgata attactctca gtgagctggg aattttattt aatattgcgc
    16801 ggggcacaaa atagatgggt acaattttta ttgctgcagg agggacaggg ggacatattt
    16861 ttccttctct ctgtattgct caagaaatga aaaagaggga tctgcaggtc atatttttgg
    16921 ggactaaacg gggaatggaa gaaaaactgg tcggagaggc gggctttcct ctcctttttg
    16981 tccgggctcg aggctggaac aggaaatggg atttttcttt tttctctatg ctgggagata
    17041 atctggtggg atttttccag actgcttttt attttttccg gtatcgtcct cgggctttac
    17101 taggcatggg aagctacctt tctctcttga gtgctgtatg ggcaagactt ttgggtgttc
    17161 ctgtttatat tcatgaacag aatatttatc ctggattatc taatcaatta gtatctcgct
    17221 gggctaagaa agtttttgtt tctgctccag agagtattac ttactggaaa gatagagagg
    17281 aaaagattga agtagtgggt aaccccttac gggaagaagt gataagttgg aaggggaaga
    17341 gagaagaggc tcggcaagaa ttaggcttgg aaggaaaaag acagactatt ctggtgatgg
    17401 ggggaagccg gggctcagag ataattaaca ccagttttct agaaattgta ggctggttag
    17461 aaaaaaaggg attacaggta atccatatta ccggtgaaga aggttttgag cgtactaaag
    17521 aaatggtaaa gaagtttccc tttccttatt taacttacaa atttctgcct caaccgggaa
    17581 tagcttacgc tgcagcagac ctggctatta cccgggcagg agctaacact atttttgaac
    17641 ttctctggtt tgaattgcca tcagtagtga ttccttatgg agaagcaacg gataatcatc
    17701 aattatataa tgctcggtgg ttagcaaaac agggattggc tcgagttata gaagaaaaaa
    17761 atttaacttc ttccttactt atagaagaga tggaaaagat tttaaattct tcctcttgtg
    17821 ttttttgtga tttaaaggga aaatatctaa aggattcagc ttttcgaatt gtacagacca
    17881 taatcgagga actaaaaagg gggaaaaggc ttggataagg cacactatct gttgccccgt
    17941 aatctccatt ttattggtat cggaggagcg ggaatgagta gcttagcatc gatagctcat
    18001 gaacaagggt atgtggttag tggctctgat attacggaga atgaagctat agctcgttta
    18061 aagagaaaag ggatacgagt ttttctgggc catcgcatgg aacaggtctg gggtgcagaa
    18121 gcagtgattg tttcttcagc cattcctgaa gaaaacgaag agttaaaaga ggctcggaga
    18181 ttaaatcttc ctattattcc ccggggagag atgttagcat ctttggtaaa cagtaaaaag
    18241 ggcattgtag tggccggaac tcacggtaaa accactacta cttctatgat ctctcttctt
    18301 ttagaagtgg cgggctggga ccctactgtt tctataggag gagaattaga agatattgga
    18361 gggaatgcta aagcaggcga gggagaattt tttatagccg aggcagatga aagcgatggc
    18421 tcttttctaa aacttaatcc tttctgtgct gtggttacca acattgaaga tgaccacttg
    18481 attttttatg gagacctgga aaaggaaaaa gaagctttta tccgtttttt gcaccagata
    18541 aaaaaagaag gatttggagt agtctgtcaa gaccaccctg ccattcgttc tatattgccc
    18601 tatctttctt ctttagaaat tttgacttat ggtatggaag aaggagatat aagaggaata
    18661 attaaagaaa aaaagaaaga tgggtttctt tttcaagtaa actggaaaga taaaaagttg
    18721 gctgattttg agttgcgaat accaggactg cacaacataa ataatgcttt agctgctatt
    18781 acggtagggt taaaattggg aatagagctg aattatttaa aaaaagccct tttttctttt
    18841 cgaggagtga aaaggcgttt cgagaaggta gggagagtaa agggagcgct ggtaatagat
    18901 gattatgctc accaccctac agaaatgttg agcgtatggc aaactgctct ggattatgcc
    18961 cgaggaaatc tggtggttat atttcaacct caccgttaca ctcgaacccg ccagttatat
    19021 cgagaaatgg ccaaagttct ggaaaaattc cagcaggttt tcattttgcc tgtttactct
    19081 gccggagaaa aacccatccc cggtgtttct gcagaattaa tttaccagga aatgaaaaaa
    19141 gatgggtttg aaaaagttca gctggtagaa agttcagaag atgcagcaga ggagtgtctt
    19201 aaaatattga agccagagga cttagtggtt actatgggtg caggagatgt atggaaagca
    19261 ggatggattc tttgctctca aaatggagga aagtaataag tcagctggga aacattcctt
    19321 atctatctat tgttcctcag ccggaaatga aagattttac cacctggaaa gttggaggaa
    19381 aggttcttgc tctcgttgaa gtgaaaagtg gagaaaattt agaccaagtc atctccttat
    19441 gtgaggagga aggaatagag tggagagttt taggacgtgg ttctaacatt ttggtaaaag
    19501 atgaaggatt tcctggagtg gtaattaagt taggaggaga atttcgcgag ttgaaaagag
    19561 aaggaggtta tatagaagga ggagcgggag ttcctcttcc tcgtcttgta aattttgctc
    19621 tagaggaatg cctgggagga atggagtttt tagtaggagt accaggaaca gtaggaggag
    19681 ctgttaggat taatgctggt tgttttggga gagaaattgg ctccatagta gaaaaagtgg
    19741 aagtacggaa gaaaaacgga aagagagaat ggctaaaaag agatgattta tgcttttctt
    19801 atcggcattc taatttaaaa aaagaaaaaa cagtaatttt aaaagccgtt tttggtcttt
    19861 tccctgaaaa tcgagaagtt ttggaaaaga gaactagaaa attttcctgc ttgcgtaagg
    19921 aaagacaacc tttagagtgg ccttctgcag gtagtgtttt tcttaatccc gaaggggata
    19981 gtgctgctcg aataatagaa gaaatgggct ttaaaggctt acgtttagga agggctcaaa
    20041 tttcttccaa gcattctaac tttattatca atttgggggg agcaaaatcc cgggaaatag
    20101 aatatcttat aaattgggta cagaaagaaa tatatataaa taaaggtgtc tggttggaaa
    20161 gagaagtaga actatggtct taatgcgaaa aacaaccaag cttttcgggc aaatcttttt
    20221 ttttattttg ttttagggtt atgttccttt gctttgttca gtttggtttt tcaaacgggg
    20281 atttttaaag taaaaaggat agagataagc ggcaaccaac agttacagga ggaagaatta
    20341 ttaaatatag ttggtgttcc tctggatagt aatattcttg ctctccgttc ctgggaagtt
    20401 gaaaagaggc tggaagaact tcctcagata aaagaagcaa aggtacgcaa ggttttccct
    20461 tatgttctgc aaatagaaat aaaagaacgg caaccagagg cctgggtaga aataggaggc
    20521 cgagcctttt gtgtggatga agaaggagta gaaatacctg cttcttcatc cacggacctg
    20581 gtaaagattc gcttatccgg gaatcaaaag aacttattat tagaatctct gaatttgatt
    20641 aaaagctgga agaaggaatt tgagcttcct ctttctgctg tggtggtaga aaacgatagg
    20701 ctgtttattt tgcagctcca aaatggtata tttataaagt gtgaaggagt taataatttg
    20761 aaaaaaaagt cggctattct caagccctat ttgagagata tatcagtaaa gtatctggat
    20821 gtaggagggt ttgaccttcg agcaggagat gatctggtta tagtgccgag tgaggagggt
    20881 gcgttttgaa gagctggcgt tccctggata gccattctcc tattttaggg tcagtggatg
    20941 taggaactag taaaatttgc actctgattg ggcagaaaaa aggcagtgct gttgaggttc
    21001 taggagtagg cttgagtagc tctcgaggta taagaagagg gaaaataata gatattaata
    21061 aggcagcagg agcgattcga gaatcgtttt ctttagctct ggaaattgcc cgtgtggtac
    21121 ctgatcttgt gtatgccgga atagccggtg atggtgtatc ttctcttaat ctggaaaatg
    21181 aagtttttct gaataaggga ggacgagaaa ttagtgcccg agatgtagac aaagctctag
    21241 aaggttctaa agcaggagta aacctggagg gcaaaaggat actgcatctt ttgcctcaag
    21301 aatacatagt ggatgatcag acaggaatag ctgacccagc aggtatgatt ggctctcgtc
    21361 ttcgagcaaa ggttcatctt attactgtag atgaaaatca atttcacaat ttttctaaaa
    21421 gtttccagca ggcaggcctg gaagttcata gattggtttt tcaacctctg gcctctgcta
    21481 tggctgtttt gcatcctatg gagaaagaga tgggtattac tcttgtggat ataggaggag
    21541 ggactgcaga tatagctata ttttacggag gttctatgcg ttattcaggg gtagttcctt
    21601 taggaggaga gagcattacc tctgatttag ctgtgggtct gcgtacttcc cgagacgaag
    21661 cggaaagaat caaattgcac catggttgcg ctttcagccg acacattccc gaagacgagg
    21721 ttattgaagc taaagatttg gggctctctt cactacagac gataaaaagg aagtatgctt
    21781 gtgagataat agaagcacgg gtaaaagaac tatttttgtt aatccgtcga gtgattaaat
    21841 cttcgggctt ttaccccttt ttgcggggag gagtggtttt gactggaggg agtgctcttt
    21901 tagagggtat tgcagaatta ggttcttcta ttttagaatt gccggttaga gtaggttccc
    21961 cggagagtaa agaccatgtg ggaatgattc aaactgtatc tcatcctatg ttttctactg
    22021 cttgtggctt gctgcaattt ggtttggaag aagtcacgga tagaagagcc agaaaatcgc
    22081 tggtccttga tttagagaat cagcggtcta aagttataga ttggtttaaa gactttttta
    22141 tgctggacta aaggcggagg gtggtagcta tgcgttcgaa ttctcaagga cttcgtaatg
    22201 ggaaagtaaa cataaaagtt ataggtgtgg gtggcggagg gggaaatgca gttaatcgta
    22261 tggttcaggc tggccttaaa ggagttactt ttgtagctat gaatactgat actcaagttc
    22321 tggaaaggtc cttggctaat gttaagttgc aaattggagc aaagctaacc cgagggttag
    22381 gagcaggtgc taatccagag gtaggtcgaa aggcagcaga agaggacaag gagaagattt
    22441 ttgaaatttt agaaggggta gatatgttgt ttgtcactgc cggtatgggc ggaggaacag
    22501 gtactggagc gtcaccggtt gttgctggac tggctaaaga attaggcatt ttgactgtag
    22561 ctgtggttac caaacctttt acttttgagg gcaaaaaaag gatgtcccag gcagaagaag
    22621 ggattaatct tttgcgggag cgagtggata gtttaattgt aattcccaat gatagactat
    22681 tacaggttgc cagcccctct acttcggtaa aggaagcttt tagtatggtg gataatgttc
    22741 ttttgcaagg agtaaaaagt atttctgacc ttatcaatat cccgggaata gtgaacctgg
    22801 actttgctga tgtcaaagct ataatggaag gagcaggaac atctcttatg ggtattggta
    22861 aagctggtgg tgaaaacagg gctagagaag cggcacatct tgcagtaaac agtcctttat
    22921 tggaaacacc tttttctggt gctaaaggag tgctctttaa tgttactggt ggtcccaatc
    22981 tcagtatctg ggatgtaaac caggtggcag aggtgattac taaatctgct tctgaagaag
    23041 ccaatatcat tttcggagca ataattgatg aaaaaatgaa agatgaagta gagattacca
    23101 ttattgctac gggttttgac caaagtaagg aagagaaaga agaaaaagtg agtgaaattt
    23161 cagactcttt ttcctttgat gacctggatg tccctgcttt cctgagaaaa aaataagtta
    23221 atggtagaaa gcgcaaaaga ttactggaag gaagagcaag ggatttctcc ctccagcaag
    23281 gtgggtaagt ttttgtttgc cctttgcttc ccgggagatt attctttagg aatgactaac
    23341 ttgggttacc agtctattat tcaccttctt tttgaagctc cagactggcg gggagaacga
    23401 tttttttatg agtatggttc tcgttcttta gaaagcaatt tacctcttaa ttcttttgac
    23461 ataatagggt tcaatttctc tttcgagcta gacattctta atcttctcct tttgttgcag
    23521 gaagggggaa ttcctctttt tgctcgagat agaaaagaaa aagacccctg ggtgataagc
    23581 ggtggccctg ctgctactct taatccggaa attattgctc ccttcatgga ccttatagtg
    23641 ataggagaag cagagaaagt tttcccttta attcttgctt tatggaaaga aggagaagaa
    23701 aaaggtgaaa gcagagaaga gaagaaaaga attctgggga aaattcccgg ggtatacatc
    23761 ccgagcaaga ttcaccctgt ttatcaacaa ggggagctcc ggggtttcga ggaagaaggt
    23821 ttttcttttc cggtaaaaag acaatgggtg aatattaatg aattcgaaac tcgtactttc
    23881 atttacaccc ctttttccta ttttcaggat gcttctctag tagagataag caggggctgt
    23941 agctatcgtt gttctttttg tgctggtaga agtatatatc accctttacg ccagcgatct
    24001 ttggaattgg tagagaaaat gattgaccat gtttctccct ggtccagtaa aataggtttg
    24061 gtaggagcag atttattatc ccaccctgct ctggaagaga taataaaata cgtactggat
    24121 aaaggtaaaa agctcaccct ttcttctctt tctgctcttt ccttggtgaa aaaaactgga
    24181 gtgagggagt tgttgtctaa gggggagatt gaaactataa ctcttgctcc ggagagcgga
    24241 agtttttctg ctcgaatgaa actgggaaag ggactttccg atgaagaatg ggtagattta
    24301 gtgagagagt tgttagaaaa gaagataaag agggtaaaac tttattttat gttgggtaaa
    24361 ccagaaggga aggtagaaga ggatctggag tttttggcta aaatggttgc tcaaataaaa
    24421 aatcctcagc aaatttcagt ttcttacagt tttttagtac ctaaacctca tactgctctt
    24481 tctgactgga ggactccttc tatttatgct tgggagagag aaaaaagaat ctgggaaaga
    24541 ggtctgggta agttaggaat tcctttttca ggggagagtc cccgcctggc ctggattcaa
    24601 ttacttcttg ctcgaggaga ccggctgata ggagagaaga ttcctcagtt attgtctcat
    24661 cctcacccct ggtcatggag gacgtggcaa tccttacttc aagggctagg tagagatgaa
    24721 gagtgggtac gttcaccctg gaaagaggta aagccctggc aggtggtaga tatggggtag
    24781 tggaaaattg ggaagcagtt aaagaaaaga ttgctatttt ttctcagcga ggaggaagga
    24841 accctgagga tataaaagta gtagcagtta ctaagggggt agaagtgagt aaaattgaag
    24901 atgcgatttc acttggatta gtctctctgg gagaaaatag agttcaagaa gcgttaaaaa
    24961 aaatgcctct attgcctcga ggtataaaat ggcatttttt aggccgcgta caaagaaata
    25021 aagtgaaaaa agtggtagaa ttttttcagt atcttcattc tttggatagt atagatagag
    25081 gagaggaact aaataataga gcaagagatt taagcaaaaa atttccggtt ttgattcagg
    25141 ttaatcttac tggtcgagag actcaaggag gggtgaaaga aaaagagtta ctgtctctag
    25201 tttatcaagt tgttaaactt tctcatttag agctgagagg ccttatgact atcggtcctc
    25261 ttggagggga agaacaagaa gtaaggaagg ttttctcccg tttacgggaa cttaaagaag
    25321 aggtgaaccg cgaaatgccc caaattacta atttccagga gctatctatg gggatgagcg
    25381 atgactttcc ctgggctatt ttagagggag ctactatact ccgtttaggt cgggctatat
    25441 ttggagagag aggtaaataa gatggaagat agcatactct ttgtcggttg tgggaatatg
    25501 ggaagtgctc taattcaggg tttatgccgg gataactggc ataaaaagtt ccgatttttt
    25561 cttctggacc aggtggcaga aaaagcgaga agtctagctt cttctttagg tttagagtgg
    25621 gtagatagct taaataactt agaaacccct cgttttgtgt ttttggctgt aaaacctaaa
    25681 gatgttaagg atgctctttc ttctcttcaa aatttttccc agtcagtttt tatttcggtg
    25741 gcagcgggag taaaaattaa agatttggag aagtttcttc ctcagcaagc ggtagtgcga
    25801 attatgccca atttgtgcgt ggaagtggga gaaggtgtag tcccggtgtg cttttctccg
    25861 gcggtgaagg ctgaagaacg agaagacctt ttgtttctct tttcttccct gggatgggtt
    25921 tttgaagcag aggaggagga tctagacctt ttcactgctc ttagtggtag tggtccgggc
    25981 tttatagctc tttttataga atcattagct gatgggggag tgaagatagg catcccctgg
    26041 gagaagagtt tgaaaatagc tactcaaacg gtattgggtg tagcatctct tttaaaaacc
    26101 cggggtatgc atcctgctca gctcaaaaat tgggtggcct ctcccggagg aactactatt
    26161 gccggaataa aaaagctgga agagggagct tttaggggat tggtgatgga aggagtggaa
    26221 gaggctttta agcgagctaa aaatatagga gaggaggaaa gaaaatgaac tggtggaaac
    26281 cggatgacgt acgagagata gattttaatc gttctttccg tggttataat gaaaatgaag
    26341 tacaggagtt tttagaagaa ctagcttctc aaatagaaag tatggtagca gagagagaaa
    26401 aaattctgca agataatcgg gaacttaaaa gaaggttggg ggaaaaagaa gaaaaacttt
    26461 ctaagatgga agcccagatg gaggagtggg aaaaacaggc agaagtagag agggaattag
    26521 ctaaaagaga atcacaaatg cttttaaaag aagcagaggt tagggcacaa aaagttgtgg
    26581 aagaagctct ggaaagaaaa aaagaaattg aaaaaggata ccaggatctt ctggaacagt
    26641 atcgtctatt tcaaattcgt ttcaggtcat tattacaaac ttttatggat agtttagagt
    26701 ggaaggaaaa ggattgggat annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26821 nccaagcagt gggctgtgga aagtttgact gaaggtacaa tcgttcttat ccccgggatt
    26881 ctcaccctta ctttacgtca taattttggt agtgcttttg gctggaaaat aatgagtaaa
    26941 gaaggattga ctctaattac tgttttcatt accattattc ttctctggat ttttattaaa
    27001 gttgtctaca ttagaggaat ctctcttttt ttagctggtg cctggggtaa tcttttagac
    27061 cgctggcgtt ttggatatgt gatagacttt atagagcctt ccttctgggc tacttttaac
    27121 ttggctgatg tttttattgt ggtagggatt attttgatcc tcttaagctt ttggtttgat
    27181 gagaagagag aagattcaag tttatgagcc ggctttttgc cggatagata tctggctgca
    27241 taaccatttt ccagaggttt ctcgttcctt aattattaag ctggttagag aaggaaaaat
    27301 aaaattaaat ggagaaacta tcaccaaggc cagtaaagaa gtataccccg gagacataat
    27361 agaagttaac tggccagtag aagatatttc tcaccctcct cctcagcttt tgcctttaga
    27421 tgttatttat gaagatgaag atatcctggt ggtgaataag agagctggat ttgctgttca
    27481 tcccactact tcctggggag gggggacttt agtaaatgct cttttttatt atcaaattcc
    27541 tctttctcaa tacggttttc ctctgcgccc tggcattgtt catcgtctgg ataaaaacac
    27601 ttccggggtg atggtaatag ctaaatccga taaagcttat cttcatctga tagaagaatt
    27661 taaagctcgc caggtgaaaa aagaatatct ggctttagtg gagggaatat gggagggaga
    27721 aaaaactatt aacctcccct tggggagaaa catttataat ccctgtcgga tggaagtaag
    27781 aaaaaaagac ggaaaagtag ctcatacaga aatagaggta gtagggaaag gaaaggagtt
    27841 ttctttattg ttagtaaagc caaaaacagg aagaactcac cagattagaa ttcatcttgc
    27901 ctcacaaggg tatccgatag tgggggatac ggtgtatgga agtaaaaaag gaaaagagtt
    27961 ttttcctcgt caagctttgc acgcttttac tctttctttt tatcatcctt cttccagaga
    28021 gtgggtttcc ttcgctgctt ctctcccgca ggatttaaga aaattcatca gtgacttcat
    28081 aaaaatttaa aggaggtttt actcttggat ttaggactta aaaataaaat agcagtaata
    28141 accggaggta gtgtaggaat tggatatgct gtagctcaag cctttgctca tgagggagta
    28201 catttagctc tctgtgctcg aggagaagaa agggtaaaag aggctgctca tactctccag
    28261 gataaatatg ggattagagc tataggagta aaagcagatg tatctaaagt agaagacatt
    28321 aataattttg tgaaggagat agaaaaagag tgggaagtag tagatatttt agttaacaat
    28381 gctggaacag gaagtgcaga gaagattatg gaagcagaag atgagaaatg gcagttttac
    28441 tgggacttac atgttatggc agcagtaagg atgtctcgag ctttaattcc attgatgaaa
    28501 aagcggggag gaggagtaat aataaataat gcttctattt gtgctcgcca acccttagat
    28561 tatgagccta tctataatgt aactaaagca gctctggcga tgttttctaa gtgtttagct
    28621 aatgaagtga ttaaagataa catcagagta aactccatca accccggtct cattcgcacc
    28681 ccggactggg ataaaacagc aaatattgtg agtaaagaaa aaggtataac tcctgaagaa
    28741 tatctggata agatagctaa agataatgct cctattggta gatttgccac acctgaggag
    28801 ctagctaatt ttttcgtttt cttagcttct gattgtgcca gttactgtgt gggttctacc
    28861 tactatgtag acgggggatg gcttaaagta gttatttagt gaaagaccaa gagataagac
    28921 aatagtatac cctaaggaga aaaagggtag caaaggattt ttggaaaaac cgcatataat
    28981 ctatagcgtg aaattcttgg aaaagataag aaaggcaaat gaaaaatacc gagtaatagt
    29041 gatagataac tttctcagtc acaggagata acttctctaa aagaacgagt tatgccagag
    29101 gttggatgaa aggattctta ttcctcatct atcaacctca taagcaggag attatattcc
    29161 ctctcttttc ttt
//
LOCUS       DAOSVE010000013        27823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_1127_length_27823_cov_69.884867, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000013 DAOSVE010000000
VERSION     DAOSVE010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 27823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27823
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1127_length_27823_cov_69.884867"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..79)
                     /locus_tag="PK016_02120"
     tRNA            complement(5..79)
                     /locus_tag="PK016_02120"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45..47),aa:Asn,seq:gtt)
     gene            complement(107..1150)
                     /gene="rpoD"
                     /locus_tag="PK016_02125"
     CDS             complement(107..1150)
                     /gene="rpoD"
                     /locus_tag="PK016_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HXL01907.1"
                     /translation="MERGKQKGYITFKELNDALGSEAINVDKLDDVYNTLLGLGIEVS
                     DEEALQEEQPTIEAEDFYFEDIKGIGVDDPVKMYLKEIGQVPLLKPEEEVELAKRVEK
                     GDPEAKRKLIEANLRLVVSIAKKYVGRGMLFLDLIQEGNLGLIRAVEKFDYRKGYKFS
                     TYATWWIRQAITRAIADQARTIRIPVHMVETINKLTRTARQLVQELGREPLPEEIAEK
                     MGITPEKVREILKTAQEPLSLETPIGEEEDSHLGDFIEDTGALAPPQAASYSLLKEQL
                     DNVLNTLTDRERKVLKLRFGLEDGRPHTLEEVGQIFGVTRERIRQIEAKALRKLRHPT
                     RSKKLRDYLDETE"
     gene            complement(1216..2967)
                     /gene="dnaG"
                     /locus_tag="PK016_02130"
     CDS             complement(1216..2967)
                     /gene="dnaG"
                     /locus_tag="PK016_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HXL01908.1"
                     /translation="MFDDQYLEEIKSRIDIVEFISHYVSLQPAGHNYRALCPFHEEKT
                     PSFLVSPEKGIFHCFGCGVGGNIFTFVMKIENLTFPEAVAFLAQKCGMELPALGKSST
                     RQTKERDKFFLLNETLNRYYYQCLLNPPEELATTACSYIYEQRKLKPEIVEQFGIGFS
                     PASGKESIAFLMEAGFSGQDIIKAGVGVVTRSGELLDRFRGRITFALHNVQGRIVGFG
                     GRSLRGEEPKYVNVSDTPIFSKGHNLYGLFASRNFIQRSHQAILVEGYMDFLTLFQEG
                     INNCVASMGTSLTRDQAMLLRRYAEEVIICYDSDIAGQKAAARGINILQEQGITVRVL
                     SIPAPHDPDSFLREVGREEFLKILEETPSLFDFQLELFIKDYGFNSLEAKAKIIRNMV
                     PFLQSIKDPVERSLKVSYLAEKLKVGESVIYSALRQEKTPEAALNSFLSKEPGKIKAE
                     KMLLRAMIESQFWRETIIKELEVKNFSRPEHRRIFRVFADSQGQGSFSPSDLIDIFSG
                     DEGLSRCVAEIMTREDLQGGVNQEVVEDLIRRLKLASVEEEMQKIQEELSREDSEERL
                     QYYHLLSQEREKLKRLD"
     gene            complement(3183..5171)
                     /locus_tag="PK016_02135"
     CDS             complement(3183..5171)
                     /locus_tag="PK016_02135"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HXL01909.1"
                     /translation="MGIAIFAPVAGLIAVIYAIFLMRRIDSFDPGNEKMQSLSKAIQE
                     GAMAFLKREYSFLVFFVIGMFILLALTRGVTEAICYVCGAICSVGAGFVGMRVATRSN
                     SRTTQAATKSVSDALTVAFSSGAVMGMCVVGFGLLGLGTLYLITKDPNVINGFALGAS
                     SVALFARVGGGIYTKAADVGADLVGKIEAGIPEDDPRNPAVIADNVGDNVGDVAGMGA
                     DLFESYVGSIVATMVLGAIPGAVYGGVSGVVFPLLIAALGVVASIIGSLFVKAKNEKD
                     LAPALNRGTYISAILTAIFVLISSLLVFRGLGTGKALGPFVATISGLIAGIAIGLISQ
                     YYTASDYKPVKQLADAAKTGPATVIINGLALGMESTVFPLLLVCAAILVAYWAAGIYG
                     IAIAGLGMLSIVAMTVSVDAYGPVADNAGGIAEMAALDPKVRKVTDSLDSLGNTTAAI
                     GKGFAIASAALTALALFVAYSQTVGLTAIDLLHPYTVVGLFIGGMMPFLFSSIVMKAV
                     GAAAFKMVEEVRRQFREIKGLMEGKAQPDYARCVDISTRGAITRMIFPGILAVAVPII
                     VGLVLGEEALGGVLAGSITTGLLLAIMMANAGGAWDNAKKYIEAGNLGGKGTPTHAAA
                     VVGDTVGDPFKDTAGPSLNILIKLMSIVALVFAPLFMR"
     gene            complement(5198..6271)
                     /locus_tag="PK016_02140"
     CDS             complement(5198..6271)
                     /locus_tag="PK016_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HXL01910.1"
                     /translation="MAKTIADIKRFWETKEKEILSPFATLSTNSRGREKEEVECELRN
                     CFQRDRDRIIHSKSFRRLKHKSQVFLSPEGDHYRTRLTHTLEVAQIARTVARALRLQE
                     DLVEAISLGHDLGHTPFGHAGEEALNEVLQPQGFRHNEQSLRVVDFLEKDGKGLNLTW
                     EVREGIAKHSKTWAEDLVFSAEELPSTPEGQVVRWADVVAYVNHDIDDALRGKVIKEN
                     ELPASALEVLGYTHRERIDRMVKDLVLNSFNQPLVKMSKEVREATEVLRRFLFERVYL
                     SERQKAERDKAKGLVKELYRFFLEHPDILEREYKLYRKGNADLQQSVCDFISGMTDRY
                     ALYIYQKYFIPSSWPWEGRRNTD"
     gene            complement(6336..9002)
                     /gene="ppdK"
                     /locus_tag="PK016_02145"
     CDS             complement(6336..9002)
                     /gene="ppdK"
                     /locus_tag="PK016_02145"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HXL01911.1"
                     /translation="MKKKYVYFFGEGDASMKLLLGGKGANLAEMTRIGLPVPPGFTIT
                     TEACTHYYKNNQSWPEGLAEEVEENLKLLEEKAGKKLGDPDNPLLVSVRSGAPASMPG
                     MMDTILNLGLNDQVVEGLAKLTNNRRFAYDCYRRFIQMFGNVVMGIGHDEFEAILDEV
                     KREVGAKLDTDLDANALRKVIDRYKELYKKATGEDFPQDPKEQLRRAINAVFESWNNK
                     RAITYRKINNIPEDWGTAVNVQMMVFGNMGFDSGTGVGFTRNPSTGEKEYYGEYLLNA
                     QGEDVVAGIRTPKPIVEIQKDLPQVFEELKKVYEILEKHYKDMQDFEFTIEKGKLYLL
                     QTRTGKRTAQAAIKIAVDMVREGLIDEKTAVKRVEPDQINQLLHPRIDRSQPLKVLAK
                     GLPASPGAAYGKVVFDADDAERLANDGEKVILVSPETTPDDIHGMAVAQGVLTSRGGM
                     TSHAAVVARGMGKPCVAGCETIKIDLDKRMFSVNGVVVKEGDYITIDGSTGEVILGQV
                     KTILPQLSSDFQTLLSWADKFRRLGVRANADTPTDAKKALEFGAEGIGLCRTEHMFMA
                     QDRLPWVQKMIMAQSPEERKEPLSKIGPMQKGDFKEIFRVMEGKPVTIRLIDPPLHEF
                     LPSLEELLVEVTTLRVKGVTGPELEEKERLLRVVQGMHENNPMLGLRGCRVGILYPDI
                     VEMQVRAIIEAACELSQEGVKVIPEIMIPLVGHRNEIKVLKEKLDEVARDVINKYQVD
                     INYSFGTMIEVPRAALVADQIAEYAEFFSFGTNDLTQMTFGYSRDDAEGKFLFFYEEN
                     GILEGDPFQVLDQEGVGQLMRMGVEKGRSARPNLKCGICGEHGGEPRSVKFCHEIGLN
                     YVSCSPFRVPIARLAAAHAALEGEESNKKFDV"
     gene            complement(8999..9859)
                     /locus_tag="PK016_02150"
     CDS             complement(8999..9859)
                     /locus_tag="PK016_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HXL01912.1"
                     /translation="MGESSSAYKVSDDFNIFVISDGTGKTATSVVQAAISQFDLPRVN
                     IMRFTHIRNREQIYSILDIVQPQKDVVAYTVVNEELAQVLRAESLNRGILAIDLLGPV
                     VEVLKKVSSRQPRGTPGLFRRLEEELSPKMEALEYAWKESSGFSISNLKDADVIILGV
                     WYPRREEGILMLAERGIKAAFLMLDPALPLPPNFEEIVACSPDKPIIGLRMNVDYLSR
                     VREERVKALGLDSLLSKAKKEVIKEELDFAQRIYDKLGCQVIDITGLSSKDLVSTIIS
                     QIKRKREEMR"
     gene            complement(9862..11958)
                     /gene="glyS"
                     /locus_tag="PK016_02155"
     CDS             complement(9862..11958)
                     /gene="glyS"
                     /locus_tag="PK016_02155"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HXL01913.1"
                     /translation="MQEKDLLLEIGMEELPISLISDIFQEWETKFAQGFRESRIDYQE
                     LKVVGTPRRMTVYLRGVAERQSPVLKEIKGPAKKVGVSEAGEFLEPAHAFARSQGIGL
                     SDIVVKETERGVYLFGVKKEEGRPVEEILPTLLPRIISSLEFPRSMLWGEGEFRFIRP
                     IRWVCALWGDQEIAFEIAGIKSSRFTRGHRFLSPEPVELQEASQYFTSLLKAKVVVNQ
                     EERRKKIEESLSREEKLVGGHWLKDESLIQEVNFLVEYPDAITGRFDLQYLRLPHRVL
                     TTVMKHHQKYFACVDDRGELLPFFFIVLNRPREGGEKIVKGNERVLKARLEDALFFFE
                     EDRKKPLRDRVEALSGIIFQESLGTMKEKVERLEKLACYLGEELGFDSGRIKRAEEAA
                     HLSKVDLACEMVKELPELQGYMGRVYALLDGEEEEVAIALEEQYYPLPGEENYPGTIA
                     GTILSLVDKMDNIVSSFSLGRVPSGSQDPWGLRRMAQGIVNIILHCQWYLSLSPWVER
                     NIFLLEKQGLGKGNGKVKTEVVEFLFNRLRLRLLSSGFNYSVINAVLSTSVDDLYEAF
                     KKIETLEKLRRERKSFLSEIVTGFTRANNISKGFSGNENLREDLLQEESEIKLYRNLQ
                     KVEKDFNQAVAGGNYLAAFEIFYSLLPLLNQFFDQVLVMCEEKDLRENRLNLMKKVVN
                     LWEDLADLSQVVIEGE"
     gene            complement(11990..12877)
                     /gene="glyQ"
                     /locus_tag="PK016_02160"
     CDS             complement(11990..12877)
                     /gene="glyQ"
                     /locus_tag="PK016_02160"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HXL01914.1"
                     /translation="MYFQDIILTLSDYWKKIGCVIVQPYDVEVGAGTMNPATFLRVLG
                     PEPWRVAYCEPSRRPADGRYGENPNRLYEHYQFQVIIKPSPSDIQDLYLKSLFHLGIK
                     SEEHDIRFVEDNWESPTLGAWGLGWEVWLDGMEVTQFTYFQQAGGIDVKPIAVEITYG
                     LERMAMYLQEKENVFEVEWNEYLTYGDIRLQEEKEHSISSFDESNPPVLFTLFGLYEE
                     EAKRLLDKKLVLPAYDYVLKCSHIFNLLDACGGISVMERNQHMGRIRQLANRCAQLYL
                     EEREALGFPFSSKIKVALL"
     gene            complement(13235..14011)
                     /gene="recO"
                     /locus_tag="PK016_02165"
     CDS             complement(13235..14011)
                     /gene="recO"
                     /locus_tag="PK016_02165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HXL01915.1"
                     /translation="MRDRFSRYWKDEGIVIRSTNLGERDRLITLFTQGKGKIKVLAKG
                     ARKIGNRFGASLDVFNYSEFMFYSGAGMPILMQGLIKFSYRELASSLGKWLEGSYLLY
                     LLDRCWQWEKPEEGLLERVLYFWEIVLRKGENVYSSFLRFKLDLAFYLGIAPHLENCV
                     SCNQDIEGEAYWSSQEGGVICPSCALRVQEKVVFPQDLRAILKYLSSPGGNSLPLRLS
                     FFQFEELDKILSDYLSYEVGEKILSWHDFQSLYQGAVFSS"
     gene            complement(14008..14892)
                     /gene="era"
                     /locus_tag="PK016_02170"
     CDS             complement(14008..14892)
                     /gene="era"
                     /locus_tag="PK016_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HXL01916.1"
                     /translation="MAFHSGFVTIWGQPNVGKSTFLNYVLGRKVAIVSSKPQTTRQVL
                     RGILNLSDAQIVFLDTPGLHSPRDEFSRWMVQEAERALIGVDVLLYMIAPHNREEEDR
                     KYLDSLHSFPGPVFLVLNKKDLVKEEEAQKILARFSPLFAFRGKAAVSSLTGEGIPEL
                     LSEVVEVLPPGSPYYEGDIISDTWERNIVAEIIREKIWWYTYQEVPYGIEVRVEEFKD
                     RGEVLYIKALIYVERESHKKIVIGQEGKMLKKIGEEARKEIEFNWGKKVYLDLWVKVE
                     KNWRKRPEVLGRWGYKSK"
     gene            complement(14898..15725)
                     /locus_tag="PK016_02175"
     CDS             complement(14898..15725)
                     /locus_tag="PK016_02175"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009215536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HXL01917.1"
                     /translation="MNRVEVANQTEESFLTRKKIQKIVDLVLKEEGKDFSGCLSIAVV
                     DAEEIRSLKKRFFHQDVYTDVISFFYGFSDKDDIWGEIIICPSVIKQEARNRGESEEK
                     ALSIVLIHGLLHLLGYEDEDAEKAKAMEEKQEILYRLLEEEEEREKLIKRAIQVRAHA
                     YAPYSCFPVGAALLGYNGDIFTGCNVENTSLGLTICAERVAIFKAVSQGIREFKKIAI
                     IGDSSSYCFPCGACRQVLYEFAPQLEVVAAKSDRDYEVVSLSSLLPQAFFLRNDRKE"
     gene            complement(15694..17802)
                     /locus_tag="PK016_02180"
     CDS             complement(15694..17802)
                     /locus_tag="PK016_02180"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HXL01918.1"
                     /translation="MKNLRKKLSLVKPSFLSFRFLNFLFYGIMLFFLFWFSSWGEEWN
                     IKEGEVANRDIIAPRSVEIIDIPSTTEAQKRILSAVPPVYRVDSQTINIVEQELEEFF
                     RSIEEARPTYSLLSTDFKDTFSKKWRITPGVLEWLITSPQEEYERVKGVTFDLINRYI
                     ANPIREEELENKILEAYSELERMDLTGNEIRVAGTLIFRFLRPTAVVDTESTQRERLK
                     ALESLQPVRRIIKRGEVILRKGEVATKEDIELLRAMGFGGEAGRWFQLLAWALVITGV
                     VVGEYFYIRRFSPALLENDSLLLLRLIAVGGVLAMNILTFRLSSFFIIISALPLILLS
                     LLGKDYALGGSLILFPLLLWGDKMDFLQGFYVYLNLFLPLFWLSKISKMRDLAKAGMM
                     MALVNMGLSFVFGLQEGKELTKVLADAFYGWGGGMGGAVIALGGIILLESTFHITSDF
                     RLMELLNPTHPLLKRLLMEAPGTYSHSLMVANLAEAAGERIGAHPFLVRAGAYYHDIG
                     KIKRPYFFIENQMEGHNIHDRLSPNLSSLIIQSHVKDGVEIARQYNLPPEIQEIIARH
                     HGTTLIRYFYDKALKERKDEVREEEFRYPGPLPHTVEEALVFLADSVEAAVRSTAHPT
                     PSRVDAIVNGILQKYLREGQLDESPLTMRDIRQIAQAFVVVLNGMIHSRVPYYPEEEI
                     LKTVRGKDVKNEPGRSSKPD"
     gene            complement(17768..18760)
                     /locus_tag="PK016_02185"
     CDS             complement(17768..18760)
                     /locus_tag="PK016_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HXL01919.1"
                     /translation="MSRGLDLKDKEIRISLESVKSIKSLFGYLDSKVKVIESVFQVEV
                     DIEKEDLVIKTKGDAGEELLARDFLQELSSFSPQEDLSSEDLQKMALSFKENKKAWWN
                     DKVEPTILTTAYHKPVRAKSPGQMNYVQAVRDADITFCIGPAGTGKTYLAMAMACAAL
                     QSKKVSRIVLVRPAVEAGESLGYLPGDLKEKIEPYLRPLYDALYEMLSPERFQKYSEK
                     GVIEVAPLAYMRGRTLNDSFIVLDEAQNTTPEQMKMFLTRMGFGSKVVVTGDITQIDL
                     PSGKKSGLVVVQEILEDIKGIEFVYLSEKDVVRHQLVQKIILAYEKFEEKTLSR"
     gene            complement(18770..19225)
                     /locus_tag="PK016_02190"
     CDS             complement(18770..19225)
                     /locus_tag="PK016_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HXL01920.1"
                     /translation="MKEVNSLKIQIQEQMKEALKAHDQVRLDTLRLLLSEIRNKEIEK
                     KGDLEEAEIIVLIQKEIKKRQESEEYFKKGNRSELLEKAEREIAILQDFVPPQLSEEE
                     IERVVDEVLANWSGEPSLGPMMKEIMSRLQGKAEGKVVSKIVRQKINVG"
     gene            complement(19222..19401)
                     /gene="rpsU"
                     /locus_tag="PK016_02195"
     CDS             complement(19222..19401)
                     /gene="rpsU"
                     /locus_tag="PK016_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HXL01921.1"
                     /translation="MTEVKVGKGESIDEALRRFKKKCQRNGVISEMKRREHYEKPSEK
                     KRKKAQAAARRKRRG"
     gene            complement(19952..20890)
                     /locus_tag="PK016_02200"
     CDS             complement(19952..20890)
                     /locus_tag="PK016_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019941234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HXL01922.1"
                     /translation="MKKVILDCDPGHDDAIAIMLACASSELEVLGITTVAGNQTGEKT
                     FINALKILTLIGKDLPVARGFDKPLLRELVTALEIHGETGLDGADLPAPLIKPWEIHA
                     VNFIVEMLLSADESVYLIPTGPLTNIAGALLLSPGIKEKIKKIVLMGGGALESNKTPA
                     AEFNIYVDPEAAKIVFESGIPITMVGLDATNKALFSLEDIEALSRMGGKVSTIAAQLL
                     TFYARQLEKFSGVKGAHLHDPLAVAAVIEPAVVDTKFLHVDIETRGEFTRGATVVDFY
                     GVTGKKPNASVALGLDLSKFKRLVFDAIKKLDNSGQ"
     gene            complement(21393..22160)
                     /locus_tag="PK016_02205"
     CDS             complement(21393..22160)
                     /locus_tag="PK016_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011752873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXL01923.1"
                     /translation="MMREKTPHVEMKDIYKSFGNVQALRGVDFTVYENEVVGLVGDNG
                     AGKSTLVKILTGVYQPDRGEIYYRGQKVKLSSPADAHALGIEPVHQSGATIDEMVVWE
                     NFFLGRELVRKFFFTELLDKKEMRRITQETLRQLGIELDSVDRPIGTLSGGQRQAVAI
                     GRAIHFGGELLVLDEPTAALSLKETEKVLKYIEGVKKLGRSVILISHLTLHVYPIADR
                     FVILDRGEKIGDFPKEQISRKELEEIIVRGRMATEVH"
     gene            complement(22172..23179)
                     /locus_tag="PK016_02210"
     CDS             complement(22172..23179)
                     /locus_tag="PK016_02210"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL01924.1"
                     /translation="MEGKMAATTGKERLVIGLAIAVILAAAGFAVGIADFIHLFILGL
                     NAMVPIVLAAVGEVLNERGGLFNIGIEGIMLLASFGAVYAAELSGSWFIGLLGGLLVG
                     ALVAYLFALIATYGAGNQVVAGVGINMLALGLVAYFLLVAWGSPGFHLLSNYEIRIPK
                     ITIWGYSVSWMIIVALGITGLIHFVLENTRFGLRLKAAGYNPFVTDVSGINVYRLRTV
                     ACVIGGVLAGLGGAYMSLDWLGLSASTLIQGRGFIALACVVFGGLDPILTLEAGYLFG
                     LLSALGLWLQNIPWAAPLMLKGGSYLFLMLPYLAVLAVLTVFPGRQRLSKEIGMPYRR
                     G"
     gene            complement(23179..24237)
                     /locus_tag="PK016_02215"
     CDS             complement(23179..24237)
                     /locus_tag="PK016_02215"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL01925.1"
                     /translation="MEMRYFWQTLKIAGGPIFAVLIGLIAGMVVLGASGYNPLYIFRG
                     LEYSFFTKHGIISALAMATPLMLTAITWAVGIRAGVFNVGAEGTLMIGAVMAIAAGTL
                     IRLPIVLHHLVVMVFAMGAGVLWTIPVAYLKIKRNVHEVVSTIMLNWVANFLVSFLVV
                     WPLRDPAWPMRSLKILPTARFQPLVAGSNLTLVFFLSLVFSLVSYVLLWHTKIGQHIR
                     ATGFNPDTARNCGINVSRATVFAFGIGGAAAGLAGYALTAGLPPLWSISEKLGTLVNY
                     GFNGICVAAIGVNHPLGIIFAAIFFGGILSSRSYMQMFLGVAPEITDVVAGVIVLILA
                     LPELSKMFGHWRRGKVTV"
     gene            complement(24253..25032)
                     /locus_tag="PK016_02220"
     CDS             complement(24253..25032)
                     /locus_tag="PK016_02220"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HXL01926.1"
                     /translation="MAGKLQAHLLIDEGDIAPYVLLPGDPGRVLRIGEKMDFFQEVAY
                     NREFRTITGNYEGIPVTVTSSGIGGPSAAIAVEELANSGAQVVIRVGSCGAYNSGVEV
                     GDVVIPSGVVREDGTSKAYISPSYPAVPDLQVLCALIKAAKDLHIPFHVGIGLSHDIF
                     YARGNELVKFWSEQGVIAADMESACVLTVGRMRNLQAGTVLTVVSAYDEAFHPWVGVK
                     DYATQTKNGAGKAMLGEEQTILLALRAIVYLDKMGGDLLES"
     gene            complement(25092..26336)
                     /locus_tag="PK016_02225"
     CDS             complement(25092..26336)
                     /locus_tag="PK016_02225"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HXL01927.1"
                     /translation="MKKFLYVILLLVFVLIAGVSSAQTQAEGIKVALVVTPPGKSDLG
                     WNFVHWLGCERAKDSGLVQEYAEVVSTETTVLSDLTLLAQSKEYDLIIVCGWEFLDAL
                     QVVANNFPDQNFIMTDVVAEFGEDEPGKFNTLSILFQQEQPSALVGALAALLAVHYDY
                     PHVGIVLGVEGPVLYEFEMGYKWGVNWAVNWVKEHHPDLAAQSKFINTPEKERVLWTY
                     TGTWSDPAKGREATEIQIRQGAVAVYAVAGSTGLGVLSYIDDHHKEQNIPFTDPPFAI
                     GVDIAQDWMNPYIVASAMKRADVAVVEAVKLIQAGTFKERIAEDNILWMNFANQGTWM
                     SDEQTLDEFMQYMVRLGSLKAEERTKILEQWKELRAAQPSWVWEAIDELREAIINGTV
                     DIPRPFGDPERWPIEELRAEYG"
     gene            26750..26932
                     /locus_tag="PK016_02230"
     CDS             26750..26932
                     /locus_tag="PK016_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HXL01928.1"
                     /translation="MALRVDEELCSGCELCVQICPDVFEMDSNGISRVKNGADESLDC
                     VEEAIDSCPTAAIIQE"
     gene            27002..27078
                     /locus_tag="PK016_02235"
     tRNA            27002..27078
                     /locus_tag="PK016_02235"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27037..27039,aa:Glu,seq:ttc)
     gene            complement(27394..27573)
                     /locus_tag="PK016_02240"
     CDS             complement(27394..27573)
                     /locus_tag="PK016_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01929.1"
                     /translation="MKWKKQLLEELPSIFSDKRAKTEKEKEELEAELYRQIGQLKVEL
                     EWLKKNLLSLSGGKA"
BASE COUNT         8068 a         6922 c         4711 g         8122 t
ORIGIN      
        1 aatctggctc cccgggcagg actcgaacct gcaaccctcc ggttaacagc cggatgctcc
       61 accattgagc taccgaggag cggctacatc aaggattata gagaacttat tctgtttcgt
      121 caagatagtc gcgcaatttt ttgctacgag tgggatgccg aagctttctc aaagccttag
      181 cttcaatctg gcgaattctt tctctggtta ctccaaaaat ctgccctact tcttctaaag
      241 tatgaggtct tccatcctcc aagccaaagc gaagctttaa aacctttctc tcccggtcgg
      301 taagagtatt caaaacatta tctaattgct cttttaaaag agagtaagaa gcagcttgag
      361 gaggagcaag cgctccagta tcttctataa aatcacccag gtggctatcc tcttcctccc
      421 caataggagt ctcaagagac aaaggttcct gagctgtttt tagtatctct ctgacctttt
      481 ccggagtgat gcccatcttt tctgctatct cttcaggaag gggctctctt cctagctctt
      541 gaaccaattg ccgagcagtt cgagtaagtt tattaatagt ctctaccata tgaaccggta
      601 tacgaatggt ccgcgcctgg tcagcaatag cccgggtgat agcctgccgg atccaccagg
      661 tagcataagt actgaattta taaccctttc gataatcaaa cttctctact gcccggatta
      721 agcctaaatt gccttcctga ataaggtcca gaaagagcat acctctacct acatattttt
      781 tagcaatact tactaccagt cgtaagttag cttcgatgag tttccttttg gcttcggggt
      841 ctcctttttc tactcttttg gctaactcta cttcttcctc gggtttcaaa agaggaactt
      901 gcccaatttc tttcagatac atttttaccg ggtcatcgac ccctatacct tttatatcct
      961 caaagtaaaa atcttctgct tctatagtgg gctgttcttc ctgtaaagcc tcttcatccg
     1021 acacttcaat gcccaaaccc aaaagggtat tataaacatc atccagctta tctacattta
     1081 tagcctcgct acctaaagca tcattcagct ctttaaaagt aatatatcct ttttgttttc
     1141 ctctttccat taaagaagaa atttccaaag aagatttatt tttattatta tcgcccaccc
     1201 gcatctctcc tctctttaat ctaacctttt caatttctct ctttcctgtg agagtaaatg
     1261 ataatattgc agcctctctt cactatcttc ccgagaaagt tcttcctgta tcttttgcat
     1321 ttcctcttct accgaagcta attttagccg acgtattaaa tcctccacca cctcttgatt
     1381 cacccccccc tgcaaatctt cccgagtcat tatctctgcc acacaacgag acaagccttc
     1441 atcaccggaa aaaatgtcta tcaaatcaga aggagaaaaa ctaccctgcc cttgagaatc
     1501 ggcaaaaaca cgaaaaatac gacggtgctc aggtcgagaa aaatttttaa cttccagctc
     1561 tttaatgatt gtttccctcc agaactgact ttctatcata gctcgtagaa gcatcttctc
     1621 tgcttttatt ttacctggtt ctttactcaa aaaagaattt aaagctgctt caggtgtttt
     1681 ttcttgcctt aaagcactat aaatcactga ttctcccact tttagtttct ctgctaaata
     1741 actaaccttt aaagacctct ccacggggtc cttaattgat tgaagaaagg gaaccatatt
     1801 tcgaataatt ttcgctttcg cttccagaga gttaaaacca taatctttaa taaataattc
     1861 caactgaaaa tcaaaaagag aaggagtttc ttctaaaatc ttcaaaaatt cctctcttcc
     1921 cacctctcgt aagaaagaat cagggtcatg aggcgcaggg atagacaaca cccgtacggt
     1981 tattccctgt tcctggagaa tgttgatacc tcttgcggct gctttctgtc ctgctatatc
     2041 tgaatcataa caaataatca cttcttcagc ataacgacgc aagagcatag cctggtcccg
     2101 ggtgagagaa gtgcccatag aagccacaca attattaatt ccctcttgaa aaagagtgag
     2161 gaaatccata tacccttcta ctagaatcgc ctgatgagag cgctgaataa aattccgcga
     2221 ggcaaacagg ccataaaggt tgtgcccttt ggaaaaaatc ggggtatcag aaacgttaac
     2281 gtatttgggc tcttcccctc tcagacttct tcctccaaac cctactattc ttccttgaac
     2341 attatgtaaa gcaaaagtaa ttctaccccg aaaacggtct aaaagttccc cgcttcttgt
     2401 aactactccc acaccagctt taattatatc ctgtcccgaa aaccctgctt ccatcaaaaa
     2461 agcaatggat tctttacctg aagctggaga aaaacctatg ccaaactgct ctactatttc
     2521 gggcttcagt ttcctttgtt cataaatata agagcaagca gtggtagcaa gctcctccgg
     2581 ggggtttaac aaacattgat aataatagcg atttaaagtt tcatttaaca gaaaaaattt
     2641 gtctctttct ttagtttgcc gggtgctgct tttccccaga gctggcaatt ccataccgca
     2701 cttttgagct aaaaaagcca ctgcttcagg gaaagtcaag ttttctattt tcattacaaa
     2761 agtaaaaata ttaccgccta ctccacatcc gaagcaatga aaaattccct tttccggaga
     2821 aaccaaaaaa gaaggagtct tctcttcatg aaaaggacaa agggcacgat agttgtggcc
     2881 tgccggctgg agactaacat agtgggaaat aaattccact atatctatac ggcttttaat
     2941 ttcttccaga tactggtcat cgaacaacaa ttaaatcacc tatttctccc cattcaggaa
     3001 gaacaataat gttaatagaa acttgctgtc cgaggttaga accccccctc ttttaaaacc
     3061 ttactcaaaa agattaatta aatttcaccc cggaaagcac tttttctctc aagagagaac
     3121 tgcataactt ctaaattaaa ttatttctcc ctcgggaata ttcccgaggg agaaataatt
     3181 tatcagcgca tgaaaagagg agcaaatacc aaagctacaa tggacataag tttaatcaat
     3241 atattaagag aaggaccagc ggtatctttg aaaggatcgc ccacggtatc tcctactacc
     3301 gctgcagcat gggtaggagt tccttttcct cctaaattcc ctgcttctat gtatttttta
     3361 gcgttatccc aggctcctcc agcattggcc atcataatag ctaaaagaag accggtggta
     3421 attgacccag ccagcactcc ccctaaagct tcttccccta aaactaaacc tacaatgata
     3481 ggcactgcta ctgctaggat tccagggaaa atcatccggg taatagctcc ccgagtgctg
     3541 atatctacac accgagcata atcaggttga gcttttcctt ccatcaatcc ttttatttct
     3601 cggaactgac gtcgcacttc ttctaccatc ttaaaagcag ctgctcctac tgccttcata
     3661 acaatagaag agaaaaggaa aggcatcatt cctccaatga acagtcccac cacggtgtag
     3721 ggatgtaaga ggtctatagc agttaaaccc acggtttgag aataagctac aaacaaggca
     3781 agagcggtaa gagctgcaga agcaatggca aaacctttac ctatagctgc tgtggtgttt
     3841 cccagagagt ctaaagaatc ggtaactttg cgcaccttcg ggtctaaagc agccatttca
     3901 gcaatacctc cggcattatc agctaccggg ccataagcat ccactgacac tgtcatagct
     3961 acgatggaaa gcatacctaa accggctata gcaataccat aaatccctgc tgcccagtaa
     4021 gctacaagga tggcagcaca gaccagaagg aggggaaaca cagtactctc catacccaga
     4081 gctaaaccat taattatcac tgtagccggg ccggttttag ccgcatctgc cagttgcttt
     4141 accggtttat aatcactggc agtatagtat tgagaaatga gaccaatggc aataccagct
     4201 ataagaccag aaatagtggc tacaaatgga cccagggctt tccctgttcc caaaccccgg
     4261 aatactaaca gagaagaaat caaaacaaaa atagcagtta gaatagcgct gatgtaagtt
     4321 ccccggttaa gagcaggtgc taaatctttt tcatttttgg ctttcacaaa gagacttcca
     4381 ataatagaag ccactactcc taaagcagca ataaggagag gaaatactac cccgcttact
     4441 cctccatata ctgctccagg tatagcacct aataccatag tagctacaat ggagcccaca
     4501 taagattcaa aaaggtcagc tcccattccg gccacgtctc ctacgttatc acctacgtta
     4561 tcagcaataa ccgccgggtt acgagggtca tcttcgggaa ttccggcttc tattttacct
     4621 accaaatctg ctcccacatc agcagcttta gtgtagatac ctcctcctac acgagcaaaa
     4681 agagcaacgg agctcgctcc taaagcaaaa ccattgatga cattcgggtc tttggtaata
     4741 agataaagtg tccccagtcc cagaagacca aatcctacca cgcacattcc cataactgca
     4801 cctgaagaaa aggcgaccgt cagggcatca cttacgcttt tagtagctgc ctgggtggtg
     4861 cgggagttac tgcgagtggc tactctcata cctacaaaac cagcacccac agaacatata
     4921 gcaccacaca cataacatat agcttccgtt actcctcgcg taagagccag aagaataaac
     4981 ataccgataa caaagaaaac cagaaaggaa tattctcttt tcaagaaagc catagctcct
     5041 tcctggatgg ctttagacaa actctgcatt ttctcgtttc ccggatcaaa agaatcaatc
     5101 cgacgcatga ggaaaatagc ataaatgaca gcaattaaac cagcaacagg agcaaaaatc
     5161 gcaattccca taatgtctac ctccctttgt aagttattta gtcagtattc cttcttcctt
     5221 cccagggcca ggaagaagga ataaaatatt tttgatagat gtataaagcg tacctatccg
     5281 tcattcctga aataaagtca caaactgatt gctgtaaatc agcgttccct tttcgataga
     5341 gtttatactc tctttctaag atatccggat gctctaaaaa aaaccgatac agttctttaa
     5401 ccaatccctt agccttatct ctttctgctt tctgtctttc actcaagtac actctctcaa
     5461 aaagaaaacg acgtaaaacc tctgtggctt ctcgtacttc tttgctcatc ttgaccagtg
     5521 gttgattaaa actatttaag actaagtcct taaccatccg gtctatccgc tcccgatgag
     5581 tatatcctaa aacttctaaa gctgaagcag gaagctcatt ttcttttata accttacctc
     5641 gcagagcatc atcaatatca tggttaacgt aagccaccac atccgcccac cgcaccactt
     5701 gtccctctgg agtagaagga agttcttcgg cactaaaaac taaatcttca gcccaggttt
     5761 tggagtgttt agcaattccc tctctcactt cccaggtcaa gttaagtcct ttcccgtctt
     5821 tttccaaaaa gtctactact ctcaaactct gctcattatg cctgaaacct tgaggttgaa
     5881 gaacttcatt aagcgcctct tctccagcat gcccaaaagg agtatgaccc aggtcatgac
     5941 ctaaactaat tgcttctact aaatcttctt gcaaacgtaa agctcgagcc acagtccgag
     6001 caatctgcgc tacttccaga gtgtgggtta agcgagtgcg atagtggtca ccttccgggc
     6061 tcaagaaaac ctgactttta tgttttaaac gtctaaaaga tttagagtga atgatgcgat
     6121 cgcgatccct ctgaaaacaa ttccgcagct cacattctac ctcttccttt tctctccccc
     6181 gactattagt gctcaaagtg gcaaatggtg ataatatttc tttttctttt gtttcccaga
     6241 accttttgat gtctgcgatt gtttttgcca ccgcttcacc cactcctttg gctttttatc
     6301 ccctacccca tttttggggc aggggataac acaattcaaa cgtcaaactt cttattactt
     6361 tcttcgcctt ctaaagcagc atgagcagca gctaaacggg cgataggtac ccggaaagga
     6421 gaacaactga catagttcag gccaatttcg tggcagaatt tcaccgacct tggctctcct
     6481 ccatgttctc cacagattcc acattttaag ttaggacgag cgctgcgacc tttttccact
     6541 cccatccgca tcaattgccc cactccttcc tggtctaaaa cctggaaggg gtctccttct
     6601 aatatgccat tttcctcata gaagaataag aatttacctt ccgcatcatc ccggctgtag
     6661 ccaaaagtca tttgagtgag atcgttagtt ccaaaggaga aaaattcagc atattcagca
     6721 atctggtccg ctactagcgc cgctcgagga acttcaatca tagtaccaaa gctgtagtta
     6781 atatctacct ggtatttatt gataacatcc cgagctactt catctaattt ttcttttaaa
     6841 accttaatct cgttgcgatg gccaaccaga ggaatcatga tctcgggaat aaccttaact
     6901 ccttcctgag atagctcgca agctgcctct ataatagccc tcacctgcat ctccacgatg
     6961 tcaggataga gaatacccac ccggcaaccc cgcaaaccca gcatggggtt attttcatgc
     7021 attccctgaa ctactctgag caatctctct ttttcttcta attctggacc ggttaccccc
     7081 ttaacccgta aagtagtcac ctctactaag agctcttcta agctgggtaa aaactcatgc
     7141 aaaggagggt caatcaaacg aatggtaact ggtttgcctt ccataacccg gaatatttcc
     7201 ttgaaatctc ccttttgcat ggggccaatt ttagataatg gttcttttct ttcctcggga
     7261 ctctgagcca tgatcatctt ctggacccag ggcaggcgat cttgagccat aaacatgtgc
     7321 tctgttcggc aaagaccaat tccctctgct ccaaattcca gagctttttt agcatctgtc
     7381 ggggtatcag cattggctcg cactcctaga cgccggaatt tatctgccca ggaaagaaga
     7441 gtttgaaaat cagaagacaa ttgaggaaga atagttttaa cctgacctaa aatgacttct
     7501 ccggtgcttc catcaatagt gatatagtcg ccttctttta ccaccactcc gttcaccgag
     7561 aacattctct tatccagatc gattttaatt gtctcacaac cagctacaca gggtttaccc
     7621 ataccccgag ctactaccgc cgcatggctg gtcattccac ccctactggt caaaactccc
     7681 tgcgctactg ccattccatg aatgtcatca ggggtggttt ccgggctgac cagaataact
     7741 ttctcaccat cattagctaa ccgctccgca tcatcagcat caaaaactac ttttccataa
     7801 gctgcaccag gagaagccgg taagcctttg gctaatacct tcagaggttg actgcggtca
     7861 atacgaggat gaagtagttg attgatttgg tcaggctcta ctcttttaac tgctgttttt
     7921 tcatcaatca gcccttctct aaccatatct acagcaattt taattgctgc ctgagcagtt
     7981 ctcttcccgg ttctggtctg caaaagataa agtttacctt tttcaatggt aaactcaaag
     8041 tcttgcatat ctttgtaatg cttttctaat atctcataaa cttttttcaa ctcctcaaaa
     8101 acctgaggaa ggtctttttg tatttccaca atgggtttag gagttctaat accggctact
     8161 acatcttctc cctgagcatt caagagatat tctccgtaat attccttttc tccggttgag
     8221 gggttacgag taaagcccac tccggttccg gaatcaaacc ccatattgcc aaaaaccatc
     8281 atttgaacgt ttactgctgt tccccaatct tcagggatat tgttaatttt ccggtaagtg
     8341 atggctctct tattattcca ggattcaaat acggcgttga tggctctacg taattgttct
     8401 ttggggtctt gaggaaaatc ttcgcctgtt gcttttttat aaagctcttt gtatctgtca
     8461 atcacttttc tcagagcatt ggcatccaaa tcggtatcta atttagctcc tacttctctt
     8521 tttacttcgt ctaaaatagc ttcaaattca tcatggccta ttcccatcac tacgttacca
     8581 aacatttgaa tgaaacggcg ataacagtca taggcaaaac gacggttgtt ggtgagttta
     8641 gctaaaccct ctactacctg gtcattaaga cccaggttaa gaatggtatc catcattcca
     8701 ggcatagaag ccggagcacc agaacgaacc gaaactaaaa gaggattatc ggggtcgcca
     8761 agcttcttac cggctttctc ttccagaagt tttaagtttt cttccacttc ttcagccaat
     8821 ccttctggcc agctttggtt attcttataa tagtgagtac aggcttcggt ggtaatagtg
     8881 aatcctggag gcaccggtaa accgatacga gtcatttctg ctaagttcgc tcccttaccc
     8941 cccagtagta atttcattga ggcgtcacct tcgccaaaaa aatagacgta tttctttttc
     9001 atcgcatttc ctccctcttt cttttaattt ggcttataat cgtacttacc aaatctttac
     9061 tgctcaaacc agttatatct attacttgac aacctaattt atcataaatt ctctgcgcaa
     9121 aatccagctc ttcctttatc acctcttttt tggctttact caacaggcta tccagaccca
     9181 gagccttgac tctttcttcc ctcacccggg acaagtagtc aacattcatc cgaaggccaa
     9241 taatcggttt atctggagag caagccacta tctcctcaaa attaggagga agaggtagag
     9301 ctgggtctaa catcaaaaaa gccgctttaa ttcctctttc tgctaacatc agtatccctt
     9361 cttctcggcg aggataccat acccctaaaa taattacatc agcgtctttc agattagaaa
     9421 tgctgaatcc actgctttct ttccaggcat actctaaggc ttccattttg ggagacaact
     9481 cttcctctaa gcgacggaaa agaccaggtg ttcctcgagg ctgacgagag ctgacttttt
     9541 tcaatacctc tactaccggc cccaaaaggt ctatagctaa tattcctcga ttaagtgatt
     9601 cggctcgtaa gacctgagct aactcttcat ttaccactgt ataagctacc acatcttttt
     9661 gaggctgcac tatatccaaa atactgtata tttgctcccg gttacgaata tgggtaaagc
     9721 gcataatatt gacccggggt aagtcaaact gcgaaatggc agcctgcact actgacgtcg
     9781 cagtcttacc cgttccatca gaaattacaa aaatattgaa atcatcgctc actttatagg
     9841 ccgaactcga ctctcccacc tttattctcc ctctatcact acctgagata agtcagctaa
     9901 atcctcccac aagtttacca cttttttcat caaatttaag cgattttctc tcaaatcttt
     9961 ttcttcacac attactaaaa cctgatcgaa aaattgattg agtaaaggta aaagagaata
    10021 aaatatttca aaagcagcga ggtaatttcc ccccgctact gcctgattaa aatctttctc
    10081 cactttttgc agatttctat ataattttat ttccgattct tcttgcaaaa ggtcttctcg
    10141 caaattctca ttcccagaaa accctttact gatattatta gcccgagtaa aaccggtaac
    10201 tatttcagat aaaaatgact tcctttctct cctcaatttt tccagagtct ctattttttt
    10261 aaaagcttca taaaggtcgt caacagaggt gctcaaaacc gcattaatca ccgaataatt
    10321 aaagccggag ctcagaagac gcaaacgaag acgattaaac aggaattcta ctacttccgt
    10381 ttttaccttt ccatttcctt ttcccagacc ctgcttttct aaaaggaaaa tatttctctc
    10441 tacccaggga gataatgata gataccactg acaatgtaat attatattca cgataccctg
    10501 agccattctc ctcaaccccc aggggtcttg agaaccactg ggaactctcc ctaaggaaaa
    10561 gctggaaact atattatcca ttttgtccac taaagaaaga atcgttcctg ctatcgtacc
    10621 gggatagttt tcctctcctg gtagaggata atattgctct tctaaggcta tggccacttc
    10681 ttcttcctca ccatctaaaa gagcataaac tctacccata taaccttgta gctcaggaag
    10741 ctcctttacc atctcacagg ctaaatccac tttagataga tgagcagctt cctcggcccg
    10801 ctttatcctt ccggagtcaa aacccagctc ttctcccagg taacaagcca gtttttctaa
    10861 tcgctccact ttttctttca tagttcccag agactcttga aaaataatac cagaaagagc
    10921 ttctactcta tctctcaagg gcttctttct atcctcctcg aaaaagaaaa gggcatcttc
    10981 taaccgggct ttgagcactc gctcatttcc ttttactatc ttttctcccc cttctcgtgg
    11041 tcgattcaaa actataaaga aaaagggtaa tagttctccc cggtcatcaa cacaagcaaa
    11101 atacttctga tgatgtttca taactgtagt aagtactcga tgaggaagcc tgagatactg
    11161 aagatcaaac cgaccggtta tagcatcagg atattccact aaaaaattga cttcctggat
    11221 taaactctca tctttcagcc agtgaccacc aacaagtttt tcttcccggc taagagattc
    11281 ctcaattttc ttccttcttt cttcctggtt aactaccact tttgccttca gtaaagaagt
    11341 aaaatactga gaagcctctt gcaactccac aggttcaggg ctgagaaaac gatgtccccg
    11401 ggtaaaacga gaagatttta ttcccgctat ctcaaaagct atttcttggt ctccccagag
    11461 ggcacaaacc cagcgaatgg gacgaatgaa tcgaaattct ccttctcccc agagcataga
    11521 acggggaaat tccagagagg aaataatccg aggaagcaaa gtaggtaaaa tttcctccac
    11581 cggtcttcct tcttcttttt tcactccaaa aagataaact cctctttcgg tctcctttac
    11641 cacgatatca gataaaccaa taccctgaga gcgagcgaaa gcatgagcgg gttctaaaaa
    11701 ttctcccgct tcagaaaccc ctactttttt ggctggacct ttaatctctt taaggacagg
    11761 gctctgcctc tctgccaccc cccggagata aactgtcatc cgccggggag tccctaccac
    11821 tttcaactcc tggtaatcta tacgagattc tcgaaaacct tgagcaaatt tagtttccca
    11881 ttcttgaaaa atatcggata ttaagctgat aggcagctct tccatcccta tctctaacaa
    11941 aaggtctttt tcttgcacta tttatccttc cctttccagc ttaaaatcac taaagtagag
    12001 caacttttat tttggaagaa aaagggaacc ccagagcttc tctttcctca agatacaatt
    12061 gagcacaacg attagctaat tgtcttatcc ttcccatatg ttggttcctt tccataacgc
    12121 ttattccccc acaagcatct aacaggttaa agatatgaga acatttcaaa acgtaatcat
    12181 aagccggaag aaccagcttc ttatccagta accttttagc ttcttcttca taaagcccaa
    12241 agagggtaaa aagaaccggg gggttgcttt catcaaaact ggatatagaa tgttcctttt
    12301 cttcctggag acggatatct ccataagtca aatattcatt ccactctact tcgaatacat
    12361 tctccttttc ctgcaaatac atagccattc tttctaaacc ataagttatc tccacagcaa
    12421 tgggctttac atctattcct ccggcttgct gaaagtaagt aaactgggta acttccattc
    12481 catctaacca aacttcccag cccaaacccc aggctcctaa agtaggagat tcccagttat
    12541 cctccacaaa acggatatcg tgttcttctg atttaatacc cagatgaaaa aggcttttta
    12601 aatataaatc ctgaatatca gaaggtgaag gtttgataat aacctgaaat tgataatgtt
    12661 cataaagccg gttgggattc tctccatatc ggccatcggc gggacgacga gaaggctcgc
    12721 aatacgctac cctccagggc tcaggcccca gaactcgcaa gaaagtagcc gggttcatag
    12781 ttcctgctcc tacttctaca tcgtaaggct gaacgatgac gcaacctatt tttttccagt
    12841 aatcactaag ggtcagaatg atgtcttgaa aatacacggc ttcctggata aataaactat
    12901 accaaccgat atagtttgct gagatatttt tattccgcct tttcccggct gtcttcactc
    12961 agcccgcggt tttaagacag cccttgcccc cttctcctta aaagagcaaa gctcaaaagt
    13021 actctatcat ttctaaaaag gcttaatagt ggcagggagg ataaagtttc cagtatttca
    13081 agattttcta tagccagact ttcagtaaac gctgcttcat tttctgctat agttgttgtc
    13141 ccgtatttag ctgaatatct tcccggtaag tataattatc ttattcctcc ctgagcacac
    13201 caagcttaga atatagcaat tttaggaaat cctgtcaact ggaaaaaacc gctccctgat
    13261 aaagagattg aaaatcatgc cagctcagga ttttttctcc cacttcgtag ctgagataat
    13321 cagaaagaat tttatctaac tcttcaaact gaaaaaagct gagtctcaac ggcaatgaat
    13381 ttccgcccgg agaagaaaga tatttaagta tagccctcaa atcttgaggg aaaaccactt
    13441 tttcttgcac tcgtaaagca caggaaggac agattacacc cccttcctgg ctactccagt
    13501 aagcttctcc ctctatatct tgattacaag aaacacagtt ctcgagatgt ggagctattc
    13561 ccaaataaaa ggctaaatct aatttgaacc gaagaaaaga agaataaacg ttctctcctt
    13621 ttctcaaaac tatttcccag aaatatagca ctctttctaa aagtccttct tctggttttt
    13681 cccattgcca gcatcggtct aaaagatata gtaaatagct tccttctaac cacttcccca
    13741 gcgaagaagc cagctctcga taagaaaatt taattaaacc ctgcatgagt atgggcattc
    13801 ctgctccaga gtaaaacata aattcggaat aattaaaaac atctaaagaa gctccaaaac
    13861 gatttcctat tttacgagct cctttggcca gaactttaat ttttcctttc ccctgggtga
    13921 aaagagtaat taaacggtct cgttctccca agttagtaga gcgaattact atcccttcat
    13981 ctttccaata acgactgaat ctgtctctca cttagactta tatccccatc ttcccagaac
    14041 ttcaggtctt ttcctccagt ttttttccac tttaacccat aaatcaagat aaactttttt
    14101 gccccagtta aactctattt cttttcttgc ttcttctcca atttttttca acatttttcc
    14161 ttcctgaccg atgacaattt tcttgtgtga ctctctttcc acatatataa gggccttaat
    14221 ataaagaact tcaccccggt ctttaaattc ctctactctt acttctatcc cgtaagggac
    14281 ttcctgatag gtataccacc agattttttc ccttattatt tctgccacta tgtttctctc
    14341 ccaggtatca gaaataatat ctccttcata ataaggagac cccggaggaa gaacttccac
    14401 aacctcagaa agcaactccg gaatcccttc tccagtgaga gaagacactg ccgctttccc
    14461 tcggaaggca aaaagaggag aaaatcgagc gagaattttt tgggcctctt cttcttttac
    14521 taaatctttt ttattaagca ctaaaaaaac cggaccgggg aaagaatgca aactatctaa
    14581 atattttcta tcctcttcct ctctattgtg aggagcaatc atataaagaa gcacatccac
    14641 acctatgaga gctctttccg cttcttgaac catccaccga ctaaactcat ctcgaggaga
    14701 atgcaaaccc ggagtatcta aaaaaactat ctgagcatcg gagagattaa gaattcccct
    14761 taaaacctgg cgagtagtct gaggcttgga agaaacaata gccactttac gccctaaaac
    14821 ataatttaga aaagtagact ttcccacatt gggttgcccc caaatagtaa caaaaccaga
    14881 gtgaaaagcc aagttattta ctcctttcta tcatttctca gaaaaaaagc ctgcggtaaa
    14941 agagaagaaa gagaaaccac ctcataatct ctatcacttt tggctgctac tacttccaat
    15001 tgaggagcaa actcataaag aacctgccgg caggctccac aaggaaaaca gtaagaagaa
    15061 gagtcaccaa taatggctat tttcttaaat tctctgattc cctgcgatac agctttaaaa
    15121 attgccaccc tttcagcaca aatggtgaga cccagagagg tattctccac attacaacca
    15181 gtaaaaatat ctccattgta tcccaatagc gctgctccta ccggaaagca agaataagga
    15241 gcataagcat gcgctcttac ttgaatagct ctttttatca atttttctct ttcttcttcc
    15301 tcttctaaaa gtcgatagag gatttcttgc ttttcttcca ttgctttagc tttttcagca
    15361 tcttcatctt cgtatcccag aagatgaagc aacccatgaa ttaaaacgat actcagtgct
    15421 ttctcttcgc tttctccccg gttacgtgct tcttgtttga taaccgaagg acaaattatt
    15481 atctcccccc agatatcatc tttgtccgaa aaaccataaa aaaaagatat aacatcagta
    15541 taaacatcct gatgaaaaaa cctttttttc aggcttcgaa tctcctctgc atccactacc
    15601 gctatgctta aacaccctga aaagtctttc ccttcctcct ttaaaaccag atctactatt
    15661 ttctgtattt tttttctggt taaaaagctt tcttcagtct ggtttgctac ttctacccgg
    15721 ttcatttttc acgtctttcc ccctgactgt ttttaatatt tcttcttcgg gatagtaagg
    15781 aacccgggaa tgaatcatcc cgtttagaac caccacaaaa gcttgagcaa tctgccgaat
    15841 atctctcata gtgagaggag attcatccaa ttgtccttct cgcaagtatt tttgtagaat
    15901 cccgttaact atggcatcca cccgactggg agtaggatga gcagtgctac gtaccgctgc
    15961 ttccacacta tccgctaaaa acaccagcgc ttcttcaaca gtatgtggta gaggcccggg
    16021 ataacgaaac tcttcttccc tcacctcatc ttttctctct tttagagctt tatcgtaaaa
    16081 ataccgtatc agagtggttc catgatgacg agcaataatt tcttgaatct cagggggcaa
    16141 attatattgt ctggctatct ccactccatc tttaacatgg ctctggatga ttaaagagct
    16201 caaattagga gaaaggcggt catggatatt atgcccctcc atctgatttt caataaaaaa
    16261 gtaaggcctc tttatcttac ctatatcatg ataataagcc ccagctctca ctaaaaaagg
    16321 atgggctcca attctttctc ctgcagcttc agcaagatta gccaccatca aactatggct
    16381 ataagtaccg ggagcttcca tgagaagtct ttttaaaaga ggatgagtag ggttcaacaa
    16441 ttccatcaac cgaaaatcag aagttatatg aaaagtgctt tctaaaagaa taattccacc
    16501 cagagcgatt accgctcctc ccatccctcc tccccatcca taaaaagcat cggctaaaac
    16561 tttagttagc tctttccctt cctgcaaacc aaaaacaaaa cttaaaccca tattcaccag
    16621 agccatcatc attcccgctt ttgccaggtc tctcatttta gagattttgc tcaaccagaa
    16681 aagaggtaag aataaattaa gatagacata aaatccttgc agaaaatcca tcttatctcc
    16741 ccacagaaga agaggaaaaa gaattaagga tcctcccaaa gcgtaatctt tacctaaaag
    16801 ggatagaaga ataagaggaa gagcgctgat gatgataaaa aaagaagaaa ggcggaaagt
    16861 cagtatgttc atggccagaa cacctcccac tgctattaag cgcagaagga gtaaggagtc
    16921 attttctaaa agggcagggc tgaaccttcg aatatagaaa tattccccca ctaccacccc
    16981 agttattact aaagcccagg ctaaaagttg aaaccaccga cctgcttctc ccccaaaacc
    17041 catagctctt aaaagctcta tatcctcttt agtagctacc tctcccttcc ttaaaattac
    17101 ttctcctcgc ttgataattc tccttaccgg ttgcaaagac tccagagctt tcaaccgttc
    17161 tctttgtgta ctctcagtat ctactaccgc agtaggtcga aggaaacgaa aaatcaaggt
    17221 tcctgctact cttatttcgt ttcccgttaa atccattctt tccagctcac tataggcttc
    17281 caggatttta ttttccagct cttcttcccg gatagggtta gctatataac gattaattaa
    17341 atcaaaggta actcctttaa ctctttcata ttcttcttga ggggaggtaa tcaaccattc
    17401 cagaactccc ggggtaattc tccacttctt actgaaagta tctttaaaat cagtagataa
    17461 aagagaataa gtaggacgtg cttcttctat agatctaaaa aattcctcta attcttgctc
    17521 tactatattt attgtttgag agtcaacacg ataaacagga ggaacagcag aaagaattct
    17581 cttctgtgcc tctgtagtac tgggaatatc gataatttct accgagcggg gggcaatgat
    17641 atctcgatta gctacttctc cttcttttat gttccattcc tctccccagg aagaaaacca
    17701 gaagagaaag aaaagcatta tcccgtaaaa aagaaaattt aaaaagcgaa aagaaagaaa
    17761 agaaggctta acgagagaga gttttttcct caaatttttc ataagctaga ataattttct
    17821 ggactaattg atgtctgact acatcttttt cactcaaata aacaaattct atccccttaa
    17881 tatcttcaag gatttcttgt accaccacta atcccgattt tttgccacta ggaaggtcaa
    17941 tctgggtgat atcaccggtc accaccactt tagacccaaa acccatacgg gtcaaaaaca
    18001 ttttcatctg ttcaggagtg gtattttgtg cttcgtccag aactataaaa gagtcattca
    18061 gcgtccttcc tctcatatag gcaagaggag ctacttcaat cactcctttt tcgctatact
    18121 tctgaaatct ttcaggagaa agcatttcat aaagagcatc atagagagga cgcagataag
    18181 gttcaatttt ctcctttaag tctccaggta gatatcccag actctctcct gcctctactg
    18241 ccggcctcac taaaacaatc ctgctcactt tcttactttg cagagcagca caagccatag
    18301 ccatagctaa ataggtcttc cccgtaccag cgggaccaat acaaaaagtt atatctgcat
    18361 ctcttactgc ctgaacatag ttcatctgtc ccgggctttt ggctcgaaca ggcttatgat
    18421 acgcagtagt caaaatagtt ggctctactt tatcattcca ccacgctttt ttattttctt
    18481 taaaactgag agccattttt tgcaggtctt cagaactcaa gtcttcttgg ggagaaaaag
    18541 aagaaagttc ttgcaaaaaa tctcgcgcta aaagctcttc tccagcatca cctttagttt
    18601 taatcactaa atcctctttc tctatgtcca cctctacttg aaacacactt tcaataactt
    18661 taactttgct atccagatag ccaaaaagtg atttaatgct tttcacactt tctaaagaaa
    18721 tacgaatttc tttatctttc aggtcaagcc cccgagacaa gtttttttat caccccacat
    18781 ttatcttttg acgcactatt ttagaaacta ctttcccctc tgctttacct tgaagacgag
    18841 acattatctc cttcatcatc ggacctagag acggctctcc agaccagtta gctaatacct
    18901 catctactac ccgctctatc tcctcctctg aaagctgggg aggaacaaaa tcttgcaaaa
    18961 tagctatctc tctttctgct ttttctagaa gttcagagcg atttcctttt ttaaaatatt
    19021 cttcactttc ctgcctcttc ttaatttctt tttgaattaa aacaattatc tctgcctctt
    19081 ctagatcccc ttttttttca atttctttat ttcttatttc cgaaagaagt aaccttaaag
    19141 tatctaacct cacctggtca tgagccttaa gagcttcttt catttgttct tgaatctgga
    19201 tttttaaaga atttacctct ttcatcctct tctttttctt cgggcagctg cttgagcctt
    19261 ctttcttttc ttttcgctgg gtttctcgta atgctcccgg cgcttcattt ctgaaatcac
    19321 tccattacgc tggcactttt tcttgaaccg gcgtaaggct tcatctatgc tttctccttt
    19381 accgacttta acttcagtca cccttattcc tccctaaatc caaagtagaa tcattataag
    19441 aaattaaaag ggaagtgtca aataaaccgg aggctgttgg aaaaatcctg ccttttagaa
    19501 agtataattc cttcctatac tcaagatgca tatctacctt taggggtctt gagctaccct
    19561 tttatactag tggatgattt tcctaccagt ggtcttttga ttttcggggg tgatgggggg
    19621 tctttttccc actaaggaag gcacttctcc ttcctcagtt gtctttgttt ttttatgctt
    19681 ttttgttttt ttgtttctgg ggattataaa ggggctttgc ccctttatag ttctgttttg
    19741 gattcctgat agaaccattc aggaatgaca gacacggagg tcttttcctc cgtgttgtat
    19801 ttgtcttttc atattttgtc tttatgtttt tctcttttcg gggggttata ggggacttac
    19861 cccctattgt tcggttataa ggggcttcac cccactgagg gaagcttttc tccctcagta
    19921 gtttttgtct ttttattttg ctttttggga gttattgacc actattatcc agtttcttta
    19981 tcgcatcaaa gacaagtctt ttaaatttag agaggtcaag ccccaaggct actgaggcgt
    20041 taggcttttt gccggtaaca ccatagaagt caacaacagt ggcacctctt gtgaactccc
    20101 ctctggtctc aatgtctacg tgcaagaact ttgtatccac aacggctggc tcaatcaccg
    20161 cagctacagc aagcgggtca tggagatgag cacctttaac accagaaaat ttttctaatt
    20221 gccgggcata aaaagttagt aactgagcag caattgtaga gactttccca cccatccggg
    20281 aaagagcttc tatgtcttct aaagaaaaca atgctttgtt tgtagcatcc aatccgacca
    20341 tggttatcgg tatgcctgat tcaaacacaa tttttgctgc ttcaggatca acatagatgt
    20401 taaactcagc agctggggtt ttgttggatt ctaatgctcc accccccata agcacaattt
    20461 tctttatctt ttccttaatc ccaggagaca ggaggagcgc accagcaata ttggtcagcg
    20521 gtccagtagg aatcagatat accgactcat cagcgctgag gagcatttct acaatgaaat
    20581 taaccgcgtg gatttcccag ggtttaatca atggtgcagg gagatcagca ccatcaagac
    20641 cagtttcgcc atgtatttcc agagcagtta caagctcccg taatagtggt ttatcaaacc
    20701 cccttgctac cgggaggtct ttcccgatca aagtcaatat tttcagagca ttgatgaagg
    20761 ttttctcccc ggtttggtta ccggctacag tagtaattcc caaaacttca agttctgagg
    20821 aagcacaggc aagcatgatg gcaatcgcat catcatgccc cgggtcacaa tcaaggatta
    20881 cttttttcat agcatcccct ctattaaaga tataatatcc cgcaaaaacc ccaactatag
    20941 ggggtaagaa ccccctttat aaccccggaa aaaaacaaaa gacggaaaaa taaaagacga
    21001 aagactacaa agacaaaaac actgagagaa aaagcgtccc tcagttataa gggggcaaag
    21061 tcccttctca tccgtcattc ctgaatgtat ttatcaggaa tccaaattat aagggaaaaa
    21121 actatttaga ttcccaataa aagatttcgg gaatgacaaa aaagggtgtc attcctgaat
    21181 acctctatta ccctttatcc gtcacccctg aatggttcta cgaggggtcc acagtattga
    21241 aaaaacctgg attcccgcct agatttcggg aatgacaaag caatactata agacctcttt
    21301 atttttccct ccctcgattt ctctttcttt tcccctcaaa aaactctttt ccctcgctcc
    21361 ccctttaatt agctatacct taaaaagtgc ttttaatgga cttcggttgc cattctgcct
    21421 cgaactatta tctcttctaa ttcttttcta gaaatttgtt ctttaggaaa atcgccaatt
    21481 ttctcccccc ggtctaaaat tacgaaccgg tcagcaatcg ggtatacgtg aagggtaaga
    21541 tgggatataa gaataacaga acgccctaat tttttgactc cctcgatata tttaagaact
    21601 ttttctgtct cttttaagga aagcgcagcc gtaggttcat ccagaaccag aagttcaccg
    21661 ccaaagtgga tagctctacc aatcgccacc gcttgcctct ggccaccgga gagagtacct
    21721 attggcctat ccaccgaatc taactctata cctaattggc gcaaggtctc ctgtgttata
    21781 cgccgcattt ctttcttatc taacagttca gtaaaaaaga attttctaac aagttcacgt
    21841 cccagaaaga aattctccca taccaccatt tcatctatag ttgctccact ttgatgtaca
    21901 ggttcaatcc ccagagcatg ggcatcagca ggtgaggaga gttttacttt ctgaccccgg
    21961 taataaatct cacctctatc cggttggtat accccggtga gaatcttcac caaagtgctc
    22021 tttccggctc cgttgtcacc taccaatcct acaacttcat tctcataaac tgtgaagtct
    22081 actcctcgca aggcctgaac attcccgaag gatttataga tgtccttcat ttccacatgt
    22141 ggggtctttt ctctcatcat ctttttctcc gctatccccg acgatagggc atgcctattt
    22201 ccttagaaag tcgctgcctt ccaggaaaaa cagtaagcac cgccagcaca gccagataag
    22261 gtaacattaa gaaaagataa cttccaccct tcaacatcaa aggcgcagcc cagggaatat
    22321 tttgtaacca aagtcctaaa gcactgagca agccaaaaag ataacctgct tccagggtca
    22381 gaatagggtc taatcctcca aacaccacac aagctaaagc tataaaacct cgtccctgaa
    22441 taagagtaga agcagataag cctaaccaat cgagggacat ataagcccca cccagaccag
    22501 ccagtacccc accaatgaca caagctacag tgcgaagcct gtaaacattt attccagaaa
    22561 catctgtaac aaaaggatta tatccggcag cttttagtcg gagaccaaaa cgggtatttt
    22621 ctaaaacaaa atgtattaac ccggttatcc ctaaagctac aataatcatc cagcttaccg
    22681 aataacccca gatggtaatt ttaggaatcc ggatttcata gttactgagt aaatgaaatc
    22741 cgggagaacc ccacgctacc agtaaaaaat aagctacaag acccagagca agcatattaa
    22801 tccctacacc ggcaactacc tggtttcctg caccatacgt ggcaatgaga gcaaagagat
    22861 aggcaactaa tgcacctacc aacaaccctc ctaagagccc aataaaccag ctcccactta
    22921 attcagctgc gtatacagca ccaaatgatg ctaaaagcat tatcccttca attcctatat
    22981 taaaaagccc tcctctttca tttaagactt cacccaccgc agccaagaca attgggacca
    23041 tagcattaag tcctaatata aacaaatgaa taaaatcagc gatacccact gcaaatccag
    23101 cagcagcaag aattacagcg atagctaaac ctattaccag ccgttctttg cccgtagtag
    23161 cagccatctt gccttccatt agactgtaac cttccctcgt cgccaatgcc caaacatttt
    23221 tgatagctct ggcaaggcga gaattaaaac tataacgcca gctactacat cagttatctc
    23281 cggtgcaaca cctaagaaca tttgcatata gctgcgcgaa cttaaaatac ccccaaagaa
    23341 aatggctgca aaaataatcc ctaagggatg atttacacca atggctgcaa cacaaattcc
    23401 attaaatcca taattgacta gtgtacctaa cttctcggaa atactccaca gcgggggaag
    23461 tccagcagtt aaagcatatc ctgccaggcc agctgccgct ccaccgatac caaaggcaaa
    23521 gactgtagcc cgggaaacat ttattccaca atttctggca gtatcagggt taaaaccagt
    23581 agcacggata tgttgtccta tcttagtgtg ccataaaagc acataagaaa ccagggaaaa
    23641 aacgagggat agaaaaaaca cgagagtcaa attacttcca gcaactaaag gctgaaatcg
    23701 agcagtggga agaattttta acgacctcat tggccatgca ggatctcgga gaggccagac
    23761 caccaggaag ctcaccaaaa aattagccac ccagtttaac attatggtag acaccacttc
    23821 atgaacattt ctttttattt tcagatatgc taccggaata gtccacaaaa ctccagcgcc
    23881 catagcgaaa accattacca ctaaatgatg caaaactatc ggcaacctga taagggtgcc
    23941 agctgcaata gccataactg cgcctatcat cagggttcct tctgctccca cattgaacac
    24001 accagctctt attcccacag cccaggtaat agctgtgagc atcagcggtg tagccattgc
    24061 aagagcactg ataataccat gtttagtaaa gaaagagtat tccagacccc gaaatatata
    24121 caggggatta taaccgcttg ctcctaagac taccatacct gctatcaagc ctattaaaac
    24181 cgcaaaaata ggaccccctg ctatttttaa agtttgccag aaatatctca tctccatact
    24241 gccacccttg cctcaacttt ccagtaaatc accgcccatt ttatccagat agacaatcgc
    24301 tcttaaggca agaagtatag tttgctcctc acccagcatt gctttaccag ctccgttttt
    24361 cgtttgagta gcgtaatctt taacccccac ccagggatgg aaagcctcat catatgctga
    24421 cactacagta agtacagtcc ctgcttgcaa gtttctcatc ctgccaacag ttaaaacaca
    24481 agccgattcc atatcagcag ctattactcc ttgctcactc cagaatttaa ccagttcgtt
    24541 accccgagcg taaaaaatgt cgtggctaag ccctatcccc acatgaaaag gtatatgcag
    24601 gtccttcgca gccttgatta aggcacataa tacctgcaaa tcaggcacag ccggatagga
    24661 aggtgaaata taagcttttg atgttccatc ttctcttact accccactgg gtattactac
    24721 atcacctact tcaacaccgg agttataagc accacaagag cctacccgta tcactacttg
    24781 tgctcctgaa ttagctaatt cttcaaccgc gatagcggca ctcggaccac caatccctga
    24841 tgaggtaact gtaactggaa ttccttcata atttcccgtt atagttctaa actctctatt
    24901 gtaagccact tcctggaaaa aatccatttt ctcacctatt cgaagaactc taccagggtc
    24961 accaggaagt aaaacatagg gagcaatatc accctcatct atcaataaat gagcctgcaa
    25021 tttaccagcc acattattac tcctctataa tatttgcagg gagtcgagcg ctcgactccc
    25081 tgcaaatcaa atcatccata ttcagctctt agctcttcta tgggccatct ctcaggatcg
    25141 ccaaacggcc ggggaatatc aaccgtacca ttgattatcg cttcccggag ctcatcaata
    25201 gcttcccaaa cccaggaagg ttgagctgcc ctcaactctt tccactgttc taaaatcttc
    25261 gttcgttcct ctgcttttag agaacctaaa cgtaccatat attgcataaa ctcatccaga
    25321 gtttgctcgt cactcatcca ggttccctgg ttggcaaagt tcatccacag gatattatct
    25381 tcagctattc tctccttaaa cgtgccagct tgaataagtt taactgcttc tactactgcc
    25441 acatcagctc ttttcatcgc actggcaaca atgtaaggat tcatccagtc ttgagcaata
    25501 tcaactccta tggcaaaggg aggatcagta aacggtatat tttgttcttt atgatgatca
    25561 tcaatatagg atagaactcc caagcctgtc gaccctgcta cagcataaac ggccactgct
    25621 ccctgtctaa tctggatctc cgtagcttca cgtccttttg cagggtcaga ccaggtgcct
    25681 gtgtaagtcc agagaactct ttctttttca ggagtattta taaacttact ctgcgctgct
    25741 aaatcagggt gatgttcttt aacccagtta acagcccaat taacgcccca tttatatccc
    25801 atctcaaatt catataaaac aggtccttca acacccagaa ctataccaac atggggatag
    25861 tcatagtgta ccgccagcaa tgcagccaaa gctcccacaa gagcgctggg ctgctcctgc
    25921 tgaaagagaa tagaaagagt attaaatttt cccggttcat cttcgccaaa ttctgccact
    25981 acatctgtca taatgaaatt ctggtctgga aagttattag ccacgacttg caaagcatca
    26041 aggaattccc agccacaaac tataatcaag tcatattctt ttgactgagc aagtagagtt
    26101 aaatcagaaa ggaccgttgt ttcagtgctc accacttctg catattcctg taccagccct
    26161 gagtctttgg cacgttcaca gcctaaccag tgcacaaagt tccaccccaa atcgcttttg
    26221 cctggaggag taacgaccag ggcaactttg attccctccg cctgagtttg cgctgatgac
    26281 acacctgcaa tcaacacaaa taccaaaagc aatattacat ataaaaactt cttcacctca
    26341 tacaccccct ttgttttaga tttctttgct cacaaaatta agtttacaag ctaagatact
    26401 atttatctaa accatctctc cacgtgctac cagaaaagct gaaatagtaa ttattgaagg
    26461 attcaactca ctatgcttct cactaacctg attaataatc atatagaatc attgtcctca
    26521 aaatagcact gcaagagctg aaagtcaagc tattaagggc tcttaacgag gagagacacc
    26581 atttttctct attgtttatc tgattcattt agaatgccgg ccctatcccc tcccgaacgc
    26641 ctcaaatact aaagaaattt tataatttga ttcatcaatt ttagataagt gtgttataat
    26701 gtcttccggt ttgcaaaaat tacactttta tcagaggagg atacagctta tggcactacg
    26761 agtagatgaa gaattatgtt ccggatgtga actatgtgtt cagatttgtc ccgatgtttt
    26821 tgagatggat tcgaacggta taagcagagt aaaaaatgga gctgatgaga gtttagattg
    26881 cgtagaagaa gccattgatt cttgtcctac tgctgctatt attcaggaat aggaaaactt
    26941 tttcttttgc cccttgcttt atataaaggg gttagtataa aataagtcat caaataaaag
    27001 cgcccccatc gtctagcctg gtaaggacac cgccctttca aggcggcggc agcggttcaa
    27061 atccgcttgg gggcgccatt atttttgtag agccattttt agcacttccc tccttctctt
    27121 aatcccgaag ggcatcgtcc ataaaaaagt ggttttgaga atggaaaaag ctttaaatat
    27181 atttcaacct aaaacctgga gagaacattt agaagagttt ctcctctaaa agcaaattaa
    27241 aggcttaaga ccacccaccc taaagaagcc tgttttttgt cttgacaatg gggtccacct
    27301 tacttttccc cataataata agaagagcgg ggaagtccta taaactcaca ttgtctatat
    27361 actggtatca gaggattcat tggctctatc attttacgct tttcctccac tcaaggataa
    27421 cagatttttt ttgagccatt caagttctac ttttaattgt cctatctgtc tatatagctc
    27481 tgcttctaat tcttctttct ctttctcagt ctttgctctt ttatcactga atatagaggg
    27541 taattcttca agtaactgtt tcttccattt cattatctga ttagaatgta ccccataggc
    27601 actggactct cttatagtct tatctccttc ggtgcttctc gtgctatttt agcttttagc
    27661 agttttgggg gtccattata cccttttttg tcattcccga aattttcaat cgggaatcta
    27721 agtttttgct ttttctttct ttttggaact gtaggggcgt cacctcccat aatttggatt
    27781 cctgatagag acattcagga atgacggact tcaagagaca ccc
//
LOCUS       DAOSVE010000014        27597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_1142_length_27597_cov_69.314247, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000014 DAOSVE010000000
VERSION     DAOSVE010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 27597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27597
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1142_length_27597_cov_69.314247"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PK016_02245"
     CDS             <1..245
                     /locus_tag="PK016_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01930.1"
                     /translation="LRLTIIKAVILMITKDTPVEEIMQKYDVLAYFLENGISPFSCAG
                     AFSQSLGKLLEIKKVKDPDAFIAGLNALIDEKERAL"
     gene            complement(487..2487)
                     /locus_tag="PK016_02250"
     CDS             complement(487..2487)
                     /locus_tag="PK016_02250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXL01931.1"
                     /translation="MTTTIYYLGFVIALLFIFFSLDDLFWDAYYYFSRWKKTRNHLQM
                     KDLDTVPRRLLAVLIPAWNEAEVIGPMVENLIRSVNYPSSLFHIFIGIYPNDSDTLRE
                     IKSLEEKYPNVHGVLNPKSGPTSKAQNLNQVLKFILHFENQKHLRFASFVVHDAEDII
                     HPTSFKLVNYLTFQYEVVQLPVFPLQPYPTWRTFFKFITSSTYADEFAENHYRGLLAR
                     DLSGAFVPSAGTGFILSHSVLDKVGRQDVFEEDSITEDYKLSFRLGELGISTHFFLEG
                     VNRVLDNGKVVTEYIATREIFPNTLREAVKQKSRWIYGISFQSFSLGKVIRDKNFSLI
                     VKYSLYRDWKAKYGNLLSIPGYVVFTYFVFSLFYVLPPIYLARSLSWWLSVVLTFLMV
                     ERQIMRGTALWKVYGWRSAIAGCLIPPLLPLRAVWGNVINFLATVSAWRICFFGFPKN
                     RPRWQKTKHSYLPPEVLLRYQRRLGDLVLEKQIVSPSILAEFLKEAKEKGEKIGEVLV
                     REGIISQEELNSILAEVLKSGYIELNNRSVRIAEITPEGIEKFKKYCVVPVAVSKYSV
                     ILAAGAPLSLEIEQEIKESLGGRNLSVVLTSPSLVAQTLQSLEEPDSWSQIPRLGEKL
                     LDQGLINEEQLVEALRAQKFYPKSLGQILVEMGVMRPEDLEN"
     gene            complement(2510..4066)
                     /locus_tag="PK016_02255"
     CDS             complement(2510..4066)
                     /locus_tag="PK016_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXL01932.1"
                     /translation="MEEQIEITPQFSETLELLENTSQSVFITGKAGTGKSTLLRVFRD
                     NTKKKIVVLAPTGVAALNVRGQTIHSFFRFKPDITQDKVKKITKKGSENIYKEVDTIV
                     IDEISMVRADLLDCVDKFLRLNSGRAKEPFGGKQMVFIGDLYQLPPVVKTKEREIFKT
                     WYETPYFFSAHAFQDLSFEFIELEKIFRQKDEIFIEILNAIRNGTITEELLAILNQRY
                     KPDYVPREGEFYVYLTSLNRLANRINEEQLARIPGSTYDLTGIIEGDFDEHDLPTERV
                     ISLKIGAQVMLLNNDPYGQWVNGSVGKVVEIEKDKSGEVKTVVVELRDGEEVYVEPFT
                     WEIFEYQYDEASASIKTHTLGSFTQFPMRLAWAITIHKSQGKTFDRIILDISGGAFSP
                     GQVYVALSRCTTLEGIVLKQPLQKKHIFMDWKVPRFLSEYQYRISEEKMPQEDKIRFL
                     REAIEEKKSLEIVYLKGNNEKSRRIIDPLYLGELDYEGKKFTGLQAYCHKRQEIRHFR
                     VDRILEIRKV"
     gene            complement(4068..5540)
                     /gene="gltX"
                     /locus_tag="PK016_02260"
     CDS             complement(4068..5540)
                     /gene="gltX"
                     /locus_tag="PK016_02260"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HXL01933.1"
                     /translation="MAEVRTRFAPSPTGFLHLGGARTALFNWALARKEGGKFILRIED
                     TDVARSTEEAVEVILDSLRWLGLYWDEGPYFQSKRLEIYRQHALLLLERGLAYECFCT
                     PEELEERRQKTMARGENWRYDRKCWNLKEEEKEKLKKEGRKSVLRFYIPPGETTFVDM
                     LRGEVHFQNEELDDFVIMKSDGMPTYNFACVVDDALMKISHVIRGDDHISNTPRQVLL
                     YQAFGFPIPQFAHIPMILGKDKTRLSKRHGSPSVTYYRDQGYLPEALFNYLARLSWAS
                     GEEEKEIFSREEIVERFSIENVTKHAAVFDLDKLNWMNSVYIRQADNKYLCSILEEIL
                     VRRGRIKPEEITPAHREYWEKVIALMKERIKYLAQLEEEASYFFTEEVEFDPPAFEQV
                     LRGEGVKEILKECEEVLSRVEDFRAPVLEEAVREMAERRGVKAARIIHPLRVAVSGKK
                     VGPGLFELLEVLGKERVIKRIQRARSLIRETCFPKNSGGE"
     gene            complement(5533..5943)
                     /locus_tag="PK016_02265"
     CDS             complement(5533..5943)
                     /locus_tag="PK016_02265"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HXL01934.1"
                     /translation="MNFVDDNNCLFCGSQNPYGFHLVFRQEDSFTVTETTVPWYYQGF
                     KGVVHGGIIAALLDEVMSHSLKNAGIEAVTGTLEARYIKPLLTEKPLITRGQIVKKEG
                     RIIETQGEVIQGGEVVARGKGVFVVPRERSEKNG"
     gene            complement(5940..7028)
                     /locus_tag="PK016_02270"
     CDS             complement(5940..7028)
                     /locus_tag="PK016_02270"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HXL01935.1"
                     /translation="MRKPWFRKPTLWIILFLLAWSLFLLWGEWQKEEVNNHYLTLAKF
                     YPQNKEKFLALFLLEEIKKDAPLEVVKTPEEGGAWEVQVTLDSQESREEFSQKMVDLF
                     RLVSSFGFVVKEEDGEKNFYSIFRGTEPWFFLETFIPPRARVALVIDDLGYNLEMAER
                     FLDLPVKLNVSIFPHLPLAKKIASLAQEKGKEVLIHFPMEAEDARENSGEAFLLRVGD
                     SEEKVQRKVEEALRLIPQAKGVNNHKGSRATSDYLLMSCFTDSLKGKGVYFLDSLTSS
                     NSLAYRLASQKGIPSFRRDVFLDGELSLSYVMTQLQKTVEIAKKNGQAVAIGHPNEVT
                     YEAIRQFVSEFSEPGVEFVFLSEMEGRN"
     gene            complement(7006..8208)
                     /locus_tag="PK016_02275"
     CDS             complement(7006..8208)
                     /locus_tag="PK016_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HXL01936.1"
                     /translation="MAEVGEWRVKSKKIWLLECVAVLLLLVIAFVWENQESKWGYGLG
                     EDINLKEWQPFFETLLLLHDNFYSDVPLEEKVLMEESIRGVLKATGDPYARYLNEEDY
                     KMENSDRVEGEFSGLGIVIAIKDNKLTVISPFQGSPADKAGIKAGDIIVEIDGMSALG
                     MSSDEAVRRMRGERGTSVTLTIQREGEEENIVVEVVRDIIKIQSVEYEILSGDIGLIR
                     ISEFHGRTQEELKKALSDLQAQGAKGLILDLRNNPGGLLQSAILTSGMFVPRGSPLLI
                     VEDREGKRESIYNFVDPVWRGPVVLVINKGTASGAEILAGVLRVHLGAELVGETTFGK
                     GVIQEIFPLSHGGGVIFTVSKYRLPDGTDLNGKGLEPDIVVSEEEEQIKKAEEELRKL
                     IDEETLVS"
     gene            complement(8196..9368)
                     /locus_tag="PK016_02280"
     CDS             complement(8196..9368)
                     /locus_tag="PK016_02280"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HXL01937.1"
                     /translation="MRRVACWVLVFLILLFLNLPGRAQDLEEEISEQQTKLEKLRSEQ
                     LKLDQELDKLLKDEKNLVKEIENLDKQIESLEKNAAQSRAKILQLEAERAELQKEIKT
                     LSQKIEEDKEKVKNILVQAYKYNGGVGFWELLMTTSDPNQWEEQWFLLRKYYQSQNYN
                     LERYLEEKGTKEEKEKELAQMIRLELVLKEQALLENQKLAKLKDTREKMRQKIASDKK
                     QFERSKNELVKAQKELENLIAKLQEDIKKRRESQPSVSIAPVKQGRLFWPVQGTIIEG
                     FGERKDPRYGISFYNPGIDIGAEKGSPVFAAHDGVVILAQTLRGYGKTILLDHGDVIT
                     MYAHLSEIKVEGGQKVKGGEVIGTVGDTGLTERPLLHFEVRVGSSAREEDPLKWLK"
     gene            complement(9365..10231)
                     /locus_tag="PK016_02285"
     CDS             complement(9365..10231)
                     /locus_tag="PK016_02285"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HXL01938.1"
                     /translation="MSKSKEAFYHRGTSYLVMGFLSVFFTQLLLNLFIFGYLELEQMG
                     GVWQSSFTVQAFFRENLSPEVIEKVKEEVETLPLVGDTRLISSEEAKSRFLNYFNLQE
                     KDLGLEENPFPPSLEVRARRVEELPLLAQELEKIEEFEEVFYGGENTEALLRFYHFFL
                     IAGGILLLALFVFSLLVVTAVTKASIQARQIEIEVWNLMGATKRFIRAPFIYQGIMEG
                     LGGGAVALVVSLVFFHFLLDFLRTSFPFFFWISWEELVLPLGVVSILMSSFLGFLGAI
                     LACNSMEEESAK"
     gene            complement(10243..10908)
                     /locus_tag="PK016_02290"
     CDS             complement(10243..10908)
                     /locus_tag="PK016_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXL01939.1"
                     /translation="MGFLEFKRVYKVYPVGVKALDNVNLSLEKGEFVFLWGATGAGKT
                     TLMKLITREELPTSGEVWLERCKISSLEERYLPVLRRNLGVVFQDLKLVPHWTVYENL
                     ISPLLVLGVKRRYAHRLAGEILELVHLQDKKYLKTEWLSGGEKQKLCLGRALIHRPYL
                     VLADEPTGNLDSATALEVVGTLFEFSKKSEATVIVATHSEDLVNRFPARLVVLDKGKI
                     IQS"
     gene            complement(10930..11667)
                     /locus_tag="PK016_02295"
     CDS             complement(10930..11667)
                     /locus_tag="PK016_02295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HXL01940.1"
                     /translation="MKTVTRELFGFSFYDCSLSELLEVLEDKIEKKEKVHVVTANPEI
                     LARGFQEEGLSEVLKEAEIVVADGIGVVMASRFLGEAINHRLPGVEIGEALMRRGEEK
                     GWRFYFLGGREEVVEKAVNELKKRHPHLEVSGFHHGYFEDDGEIIEDINRANPHILFV
                     GLGSPRQEYWIQKNRERINALVMIGVGGSFDVWSSTKKRAPQWMRKLNLEWAYRVICE
                     PRRLRRVIPAFFNFARVVWKDKRNQMV"
     gene            complement(11664..12701)
                     /locus_tag="PK016_02300"
     CDS             complement(11664..12701)
                     /locus_tag="PK016_02300"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HXL01941.1"
                     /translation="MLGYYGFGNWGDELSLRAVLEDLERIGEELSISLLYQVLYRESN
                     FLLPLPENTHPVKRDNFFSILQVIKNSDYILVGGGSLLQDVTSFRSLLYYFSLLRWAQ
                     WWNKKVIFYRVGLGPLIRRESRFLVSHLLKKSSLSIFRDEESLSLALELGSASPFLGA
                     DPLFSWKGKEKKGKGEKIALFIRSFPEEKKEALCRAFLQFSEVEGENLEVVAFHPEKD
                     GEIAEEVAKSLNCSWVIMSQCSQIIPYFEKLKTIFTLRLHPAILATLLDIPWFALDLD
                     PKIRAFSKWWKGENLLSFSDLEENKLEEVYQKRDTLREKGKEIKRELGQKSEESVKIL
                     EEFLRKAEKMK"
     gene            complement(12709..14583)
                     /locus_tag="PK016_02305"
     CDS             complement(12709..14583)
                     /locus_tag="PK016_02305"
                     /inference="COORDINATES: protein motif:HMM:NF039831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5693 family protein"
                     /protein_id="HXL01942.1"
                     /translation="MRRNIFLLVGFLIIFFFSLPILSERWERESQSKVVELVVDWNDI
                     EWVGKIEGTDPLLLAEQLKKEGFDTLGISELTWRQLVSQGRAVSVAPSPFSSPLLRYF
                     QISDPLLRETVKNHLLLLGKEVEEKGEILGVNILYEEEDKIGVGWDYRVLQSLQEQGF
                     HLILRPYNRYNIPPSVLENLLQEKVWEVAEGVIFSGDSVLGAPRLDSLRLLSSFLEEK
                     GLFWGYLEFVGQKGESTLASLVSPFTLRAHSIPPEELKGYTPVEARDRFLRAVKERSV
                     PILYLRLFTDPPGFGNWDKNLSYLVSVKKALTDNGYTVGKVSVPFPSFSPPLYSLIFF
                     SLALSFFAFYFWGSFFRSSFWVIPLCFIPLAGLIFYNPLWGMRVLGLLAGIIYPTLGV
                     IILIDTIFSGKGKLQGLGWAFITLFSGALTVSLGLYNWLFVLRIYQYWGVKVSLLVPP
                     LLTLLYLFRKGYWGLSIRQVLLEDLKRFELLVVAFLGVAALLYLTRSGNFPLLPAGGL
                     EIRIRALLERLLFMRPRTKEFLIGYPALWLLFYWNKDKVRPSYSLILWLGVAVGFATF
                     FNSFCHIHTPFLYILLRFLNALVLSLPVAVIYIIAIKLALFLWQRVGRWGERSHSAEN
                     "
     gene            complement(14580..14936)
                     /locus_tag="PK016_02310"
     CDS             complement(14580..14936)
                     /locus_tag="PK016_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HXL01943.1"
                     /translation="MRKFLVRVNGEEYEVEVAEVREVEENHWNIRNIKKVVPSSSTLQ
                     VTTTLRAPMPGRVLAINVAKGEKVETGQVVIILEAMKMENEIAVERGGVVKEVYVEEN
                     DTVDSGDPLLEIEEER"
     gene            complement(14940..15128)
                     /locus_tag="PK016_02315"
     CDS             complement(14940..15128)
                     /locus_tag="PK016_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01944.1"
                     /translation="MVISQYDLGVGLNPQVVAAIVAAIFQILDEENKPSSSYVPKKSK
                     NWKELGKHERCWVLRLRR"
     gene            complement(15109..16668)
                     /locus_tag="PK016_02320"
     CDS             complement(15109..16668)
                     /locus_tag="PK016_02320"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HXL01945.1"
                     /translation="MPKKTLLELVEELKKEKALLLQGGGEKALKKQHEAGKLTARERI
                     ELLVDEGSFEEIDLFVEHRADRFGMAERKLPADGVITGFGTIEGRVVFVYSQDFTVMG
                     GSLGEMHARKITKIMDMALKVGAPLIGINDSGGARIQEGIDSLSGYGGIFFRNTQASG
                     VIPQIALILGPCAGGAVYSPALMDFIFMVEGESKMFVTGPQVVKAATGEEISAEELGG
                     AATHATKSGVAHFVASTEEECFLEVRTLLSYLPSNNVEEPPVKDSSDPWDRDNSPLLE
                     IVSPHPNKPYDMKEVIEELVDKDSFLEVQKDFARNIIVGFSRIGGQVVGVVANQPYHL
                     AGCLDIDAADKASRFIRFCDCFNIPLLTLVDVPGFLPGSNQELGGIIRHGAKMLYAYS
                     EATVPKITVILRKAYGGAYLAMCSRDLGADLVLAWPTAEIAVMGAEGAVDIIFRQEIE
                     KALDSKAEREKLLEQYRQEFYNPYQAARRGYVDEVIDPRETRIKIAKALEVFWSKREE
                     GKGKKHGNFPV"
     gene            complement(16661..17488)
                     /locus_tag="PK016_02325"
     CDS             complement(16661..17488)
                     /locus_tag="PK016_02325"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HXL01946.1"
                     /translation="MKVYTLIGPSGTGKSYHVPHLAQKYKIDCIIDDGLLISEGRIIA
                     GRSAKAEINKIRATKIALFYFSQDRQAVKRALKKLAPQNIMILGVSLPMVEKICEHLE
                     LPPPEEILEIEKIASPQEIELALSARENEGKHTIPVPLVELKRNLWNSLVDTVPVTIW
                     RWFYSSREKTVVRPPFSYLGKLVVSEKALLSLLNFFLSRFPWIEEIKGLSIRQFEGGV
                     EIVVNCVINSGFSLSQAGHLTQKRLKEGLEYLTGVDVRAVNVNVEGIADREEKLTYA"
     gene            complement(17494..18598)
                     /gene="prfB"
                     /locus_tag="PK016_02330"
     CDS             complement(join(17494..18534,18536..18598))
                     /gene="prfB"
                     /locus_tag="PK016_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_143156243.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HXL01947.1"
                     /translation="MPDVIEEIEEVKSKLEEVGGYFDQLKLEEKIKEIEKKMSQEGFW
                     DNRNGENQILGEYKSYRQIWERYQNLQERLREAQEFSSLINEADPEFSELRKEIRSLV
                     QEAEDLKIQLLFSEVEDSRNAIVTIHSGAGGVESQDWVEMLFRLYLRFCERKGFEVRV
                     TDILEGEEAGLKYVTFLVQGEYAYGYLKSEKGVHRLIRLSPFDANHRRHTTFALVEVT
                     PEMGDDIKVEIDPQDLKIETFRAGGHGGQHVNVTDSAVRIIHIPTGIVVQCQNERSQH
                     QNKALAMRILKSRLYEYYQEEQRKKIQEIKGERKDIAWGNQIRTYVFHPYSLVKDHRT
                     GHETGNVERVMNGEIDDFIEVFLEKRQISPEKG"
     gene            complement(18612..21290)
                     /gene="secA"
                     /locus_tag="PK016_02335"
     CDS             complement(18612..21290)
                     /gene="secA"
                     /locus_tag="PK016_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HXL01948.1"
                     /translation="MSWIKDFWADRAQAKQIRELQDKYVEAVNAREKEVSHLSDEELR
                     QKTEEFKRRIQEGSSLDDLLVEAFAVVREAARRITGMRPFDVQVMGGVVLHQGKIAEM
                     ATGEGKTLVATMPAYLNALEGKGVHIVTVNDYLAKRDRYWMGPIFEFLGLSVGLIQHD
                     SSPAERKKAYLCDITYGTNTEFGFDYLRDNMVIRFEDVVQRELNYAIVDEVDSILIDE
                     ARTPLIISGPAEENTEIYYKVDKVVRKLSYPEDFDYEEKTRSIWLTERGVTKVENLLH
                     IDNLYEETGKDSLEHLVRQALRAHHLYKKDVDYVVKDGEVIIVDEFTGRLMHGRRYSD
                     GLHQAIEAKEGLRVANENQTLASITYQNYFRMYNKTCGMTGTAKTEESEFIYTYGMPV
                     VVIPPNRPLRRTNHPDVIYRKEEEKFAAVVEEIEKQHKAGRPVLVGTVSIEKSEKLSK
                     LLHHRKIPHQVLNAKHHEKEAEIVAQAGKKGAVTISTNMAGRGTDILLGGNPDFLARE
                     EMRNRGFKEEIILEAAEGLPTNLPEVVEARKLYRELLSQFKETTEREGEEVKAAGGLH
                     VIGTERHESRRIDNQLRGRAGRQGDPGSSQFFLSLEDDLLRLFGSDRISQLMEKLGME
                     EGVPLEHPLVTRAIESAQKRVEGHYFQIRKTLLQYDDVMSKQREVIYGQRRTVLREKN
                     LRPYILGMMEEVIKDIVSAYAPEKVYPEEWDWEGLSQRLFSVLGWEWRVNEEKKLNYS
                     PSLLQEDILQSLREAYEKRVQEIGEPLFQDLERYVLLRVVDNYWKDHLRNMDHLREGI
                     GLRAVGQRDPFVEYQLEAFDMFEDMIAHIREDTIRYLLHLQVVAEAKKSSPRVSPSRA
                     PREKVTTVRSKNKVGRNDPCPCGSGLKYKYCCGKNE"
     gene            complement(21624..22415)
                     /gene="rsmA"
                     /locus_tag="PK016_02340"
     CDS             complement(21624..22415)
                     /gene="rsmA"
                     /locus_tag="PK016_02340"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HXL01949.1"
                     /translation="MSSFHHSRRWGQHFLIDKAVRDIIIDEAELEKEDTVVEVGVGEA
                     VLTEILVQKAGRVIGWEIDEQLYRMVQEKLKSYSNLVLYREDFLSADLPSLLSPFSPL
                     KLVSNIPYAISSPFFSKILESNIRWNLLILMVQREFGEKILQEKGSPLSVGVNLLYKV
                     EKIKEVSPGSFSPPPAVHSLILKFTPLSSQIEPDTYKEIMRWVHFLFSQRRKKIEKLL
                     RPKLGKEGAKEILAQARINPEWRAEDLMVEHWMALSSQLRTKNND"
     gene            complement(22654..22998)
                     /locus_tag="PK016_02345"
     CDS             complement(22654..22998)
                     /locus_tag="PK016_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01950.1"
                     /translation="MPFSEEPGEIKVSVSTLVKMAARSAFPVEGVKGDFDYLLKNTKV
                     MVRGEALSPNDRVSTKEEVFINISLKLDSRANWREVAQEVQKRVANAIREWEFPLRGV
                     NIEVAEVEWVLE"
     gene            complement(22988..23479)
                     /locus_tag="PK016_02350"
     CDS             complement(22988..23479)
                     /locus_tag="PK016_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HXL01951.1"
                     /translation="MNRNLIIGVIGQHSCDAQLYEIAYQVGKGIAERGAILLCGGLGG
                     VMEAACRGAKEAGGFTIGVLPGNFEGDANPYVDLKIVTGLGEVRNLIIVLTASALIAC
                     GGQFGTLSEIAFALSHGKVLAGLNTWKIQDSTGEGDFFPQFQNAEAALNYVWKKLERN
                     NAF"
     gene            complement(23469..23960)
                     /locus_tag="PK016_02355"
     CDS             complement(23469..23960)
                     /locus_tag="PK016_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HXL01952.1"
                     /translation="MIRMKVQGLMFDQKNAMAVVVLSDEEEKRSLPIWIGLFEAQAIL
                     LGLQGVKTPRPLTHDLIASLIHQLGGELEKIVITKIVDNTFYALLYIQVEGNEVTVDA
                     RPSDGIALSLRMGAPIFASEEVLSSTTVATGEIDDKEIEEFRQFLDNLKPEDLKKYLG
                     YES"
     gene            complement(23981..24775)
                     /locus_tag="PK016_02360"
     CDS             complement(23981..24775)
                     /locus_tag="PK016_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HXL01953.1"
                     /translation="MSFWVDVHAHLEGEEFLKDREEVINRAQSAGVEIIVNAATSLPS
                     VQETLKLVERYPSLYASVGIHPQEIGDELPDFSILEEYVQKEKVLAIGEVGLDYYWDR
                     SHIKKQKEAFIQQIELAEKFSLPLIVHSRESNRDLWQIIKERVREVVVVWHCFSGDEE
                     ILQEALLQPVYFSLGGVVTYPKATHLREVVKKIPLSRVLLETDSPYLPPQSRRGKRNE
                     PAFLIETASFLAQLWGISLLTLREKVWENFYSIFGRKLNLRYNNRM"
     gene            complement(24772..26647)
                     /gene="metG"
                     /locus_tag="PK016_02365"
                     /pseudo
     CDS             complement(24772..26647)
                     /gene="metG"
                     /locus_tag="PK016_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
     gene            complement(26719..27201)
                     /locus_tag="PK016_02370"
     CDS             complement(26719..27201)
                     /locus_tag="PK016_02370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HXL01954.1"
                     /translation="MAEDLSPREREEVLEFLYQCLMEKRIFVFSTTPQFGRKCLEKNP
                     GGMVITGHYSVSERKLARVAAKYVGGCGAGRAYCALQPDGVITPCVFMPIASGNIIEQ
                     SFSQIWKESVELKDLGDRTLYGGHCENCDYRLVCGGCRVRTYAYFKDYLAPDPGCKFN
                     "
BASE COUNT         8125 a         6818 c         4502 g         8152 t
ORIGIN      
        1 ttttgcggtt aactataata aaggcggtga ttttaatgat tactaaagat accccggttg
       61 aggaaattat gcaaaaatat gacgttcttg cttatttctt agaaaatggg atctctccat
      121 ttagctgtgc cggtgccttc tctcaaagtt taggtaaatt gctcgaaatt aaaaaagtaa
      181 aagaccccga tgcttttata gcaggactta acgcattaat cgatgaaaaa gagcgggctt
      241 tataaagccc gctcttttat aactctttta taacaacata aggtagatag tcttttccgt
      301 ttactcgact taatcagata aaaacatgtt tgtaatagta atgcaatatt ctgaatagtt
      361 ctggtttcac tgcagtatat aaggatagtt gttttgtgct tacatcccag gtaaaaattt
      421 gacgggaatc atcccctcgc tgaggggcca atatctccag ccaatcataa ctaaaaagaa
      481 aaatagtcaa ttctccaggt cttcaggcct cattacaccc atctctacca atatttgtcc
      541 caaggattta gggtagaact tctgggctct caaggcttct accaattgtt cttcgttgat
      601 taatccctga tccagaagtt tttctcctaa tcgagggatt tgagaccaag agtctggctc
      661 ttccaaactt tgaagtgttt gagcgacaag agagggactg gttaaaacta ccgagagatt
      721 ccttcctccc agactctctt ttatttcctg ttcaatctct aaagaaagag gagctccggc
      781 agctaaaatt acggaatact tgctcaccgc tactggcaca acgcaatatt ttttaaactt
      841 ctctattcct tcgggagtaa tttctgctat tctaaccgaa cggttgttca gctctatata
      901 gccacttttc agaacctctg ccaaaatcga gttcaactcc tcttgagaaa taatcccttc
      961 tctaaccaga acttctccta ttttttctcc tttttccttt gcttccttaa gaaattcagc
     1021 taaaattgat ggagatacaa tttgtttttc caatactaaa tctcctaacc tacgttggta
     1081 acgcagtaga acctcagggg gaagataaga atgttttgtt ttctgccacc gaggacgatt
     1141 tttgggaaaa ccaaagaaac atattctcca agcagaaaca gtagccaaaa aatttataac
     1201 gtttccccat accgctcgta gaggtaaaag aggaggaatc agacaaccgg ctatggctga
     1261 gcgccatcca taaactttcc acagggctgt acctcgcatt atttgccgct ccaccattaa
     1321 aaaagtgagc actacactca accaccagga taaagaccgc gccaggtaaa tagggggtaa
     1381 aacgtaaaac agggagaaaa caaaataagt aaagactaca tatccgggaa tagagagaag
     1441 attcccatat tttgctttcc aatcccggta aaggctatac ttaacaatga gagaaaaatt
     1501 tttatccctg ataactttcc caagagaaaa gctctgaaaa gaaataccat aaatccagcg
     1561 cgacttctgt ttgacagcct cccgaagagt attagggaaa atttctcgag tagctatata
     1621 ttctgtgacc actttcccat tatccagcac tctattgacg ccctccagaa aaaaatgagt
     1681 agaaataccc agctcgccta agcgaaaaga taatttatag tcttcagtta tactatcttc
     1741 ttcaaacaca tcctgccttc ctaccttatc taacacagaa tgagaaagaa taaaccccgt
     1801 ccctgccgaa ggaacgaaag caccgcttaa gtctcgggcc aaaagccccc gatagtgatt
     1861 ttcagcaaat tcatcagcat aggttgaaga agtaatgaac ttaaagaaag tacgccaggt
     1921 aggatagggt tgaagaggaa aaactggtaa ctgtaccacc tcatactgga aagttaaata
     1981 gttgaccagc ttgaaagaag tagggtgtat tatatcttca gcatcatgga ccacaaaaga
     2041 agcaaaacga aggtgttttt gattttcgaa atggagtata aatttcaaaa cctgatttaa
     2101 gttttgagct ttgctggttg gtcccgactt ggggttcagt accccatgca cattgggata
     2161 cttttcctcc aaacttttta tctccctcaa ggtatccgag tcattgggat aaataccgat
     2221 gaaaatatga aaaagcgaag aaggatagtt gacagaacga atcaaattct ccaccatagg
     2281 accaataact tctgcttcat tccaggctgg gattaaaacg gctaacaagc gccggggaac
     2341 agtatctaaa tctttcattt gcaggtgatt tctggttttt ttccatcggg aaaaataata
     2401 ataagcatcc cagaataggt catccaggct aaaaaagatg aataaaagag caatgacaaa
     2461 tcccagataa taaatcgtcg tagtcaaaga aatagtttac tccaattatt tatacttttc
     2521 tgatttctag aatgcggtct acccggaagt gtcttatttc ttgccttttg tggcaataag
     2581 cctgaagacc agtgaatttt ttgccctcat aatctaattc tcccagatag agagggtcaa
     2641 ttattcgtct cgacttttca ttatttccct tgaggtaaac aatctctaaa ctctttttct
     2701 cctcaatagc ttcccgaaga aagcggattt tgtcctcctg aggcattttt tcttccgaaa
     2761 tccgatattg atattcactc aggaagcgtg gcactttcca atccataaag atatgcttct
     2821 tctggagagg ttgcttaagc acgattccct ccagagtggt acatcggctc agagctacat
     2881 agacctgacc gggggaaaaa gcgcctccgg atatgtctag aattattcgg tcaaaagttt
     2941 taccctgact tttatggatg gtaatagccc aagctaaacg catcggaaac tgggtaaaag
     3001 aacctaaagt gtgagtttta atcgaagctg atgcttcatc atattgatac tcaaatattt
     3061 cccaggtaaa aggctcaaca tatacctctt ccccatctct caactctact accactgttt
     3121 tcacttcccc ggatttatcc ttttcaattt ctaccacctt tcccacactt ccatttaccc
     3181 actgaccata ggggtcatta ttcaagagca taacctgcgc tcctatcttc aaagagatga
     3241 ctctctctgt gggaaggtca tgctcatcaa aatcaccttc tattatcccg gtaaggtcat
     3301 aagttgaacc ggggattctg gctaattgct cttcatttat ccggttagcc aagcggttga
     3361 gggaagtaag gtagacatag aattctccct cccggggtac gtagtcaggt ttatacctct
     3421 ggttaagaat cgccaaaagt tcttcagtaa tagttccatt acgaatggca tttaaaatct
     3481 cgataaatat ttcgtctttt tggcggaata ttttttccag ctctataaac tcaaaagaca
     3541 aatcttgaaa agcatgagca gagaaaaaat aaggcgtctc ataccaggtt ttaaaaattt
     3601 ctctctcttt tgtcttaact accggaggaa gctgataaag gtcgcctata aaaaccatct
     3661 gttttccacc aaagggctct ttcgccctac cactattaag gcgcaaaaac ttatctacac
     3721 aatctaaaag gtcagctctc accatcgaaa tctcatctat tacgatggta tccacttctt
     3781 tataaatatt ttcactgcct tttttagtaa tcttctttac tttatcctga gttatatctg
     3841 gtttaaaccg gaaaaaagag tggatggttt gccctcgaac attcaaagca gcaacgccag
     3901 taggagctaa aaccactatc ttctttttgg tattatcacg aaagactcgt agaagagtgg
     3961 attttcctgt ccccgctttt ccagtaataa aaacactctg cgatgtattt tctaaaagtt
     4021 cgagagtttc ggaaaactga ggagtaatct caatttgctc ttccacatca ctcaccacca
     4081 ctatttttag gaaagcaagt ttctctaatc aagcttctgg ccctctggat tctctttatc
     4141 actctttctt ttcccaatac ctcgagaagc tcgaaaagtc ccggacctac ttttttcccc
     4201 gataccgcca cccgcagagg atggataatc cgtgctgcct ttacccctcg cctctccgcc
     4261 atttccctca ctgcttcctc taaaaccggt gcccgaaaat cctctacccg agaaagcact
     4321 tcctcacatt cttttaatat ttcttttact ccttcccctc ttaaaacctg ctcaaaagca
     4381 ggagggtcaa actccacttc ctcagtaaaa aagtaactgg cttcttcttc cagttgagct
     4441 aagtatttaa tcctctcttt catcagagct atcacttttt cccagtattc ccgatgagca
     4501 ggagtaattt cttctggctt tatccttccc ctgcgcacca gaatctcctc taaaatagaa
     4561 cacagatatt tattatctgc ctggcgaata tagacgctat tcatccagtt tagcttatcc
     4621 aggtcaaaaa ctgcagcatg cttagtaacg ttttctatag aaaatctttc tactatctcc
     4681 tcccgggaga atatttcctt ctcctcttct ccgctagccc agcttaaacg agctaaataa
     4741 ttgaaaagag cctcaggaag ataaccctgg tctcgataat aagtaaccga aggagaacca
     4801 tgcctcttac ttaaacgagt tttatcttta cctaaaatca tgggaatatg agcaaattga
     4861 ggaataggaa agccaaaagc ctgataaagc aaaacttgcc ggggagtatt ggaaatatgg
     4921 tcatcacccc ggattacatg agaaatcttc atcagggcat catccaccac acaagcaaaa
     4981 ttgtaggtag gcattccatc tgatttcata attacaaaat catctaattc ctcattctgg
     5041 aaatgaactt ctcccctcaa catgtctacg aaagtagttt ccccaggggg aatataaaag
     5101 cgtaaaactg actttcttcc ttctttttta agcttttctt tttcttcttc ttttaaattc
     5161 caacacttcc ggtcatagcg ccagttctcg cctcgagcca tggttttttg ccttctctct
     5221 tctaactcct caggagtaca gaaacactca taagctaaac ctctctctag aagcaagaga
     5281 gcatgctgtc gatagatttc caggcgttta ctttgaaagt aagggccttc atcccaatac
     5341 agccctaacc accttaagct atctaaaatc acctctactg cttcctcagt agagcgagct
     5401 acatcggtat cttcaattct taaaataaat tttccacctt cttttcgagc caaagcccaa
     5461 ttaaatagag ccgttcttgc tcctcccaga tgtaaaaatc cagtaggact gggagcaaat
     5521 ctagttctca cttcagccat ttttttcact cctttctcgg ggtacgacaa aaactccttt
     5581 tcctcgagcc actacttctc ctccctgaat tacttctcct tgtgtttcta ttatccttcc
     5641 ttctttttta actatttgac cacgagtaat gagaggcttt tcggtcaaaa gaggttttat
     5701 gtacctggct tctaaagtac cagttactgc ttctattccc gcatttttca agctgtgaga
     5761 cataacttca tctaaaagag ctgctattat tcctccatgc actactccct taaatccctg
     5821 ataataccag ggaactgtgg tctcagtaac ggtaaaacta tcttcctgtc taaacactaa
     5881 atgaaaacca taaggatttt ggctcccaca gaataagcag ttattatcat ctacaaagtt
     5941 caatttcttc cctccatttc cgaaagaaaa acaaattcta ctcccggctc agaaaattca
     6001 cttacaaact ggcgaatagc ctcgtaagtc acctcattgg gatggccaat agccacagcc
     6061 tgtccatttt tcttcgctat ttctactgtt ttttgtagtt gagtcataac ataagaaaga
     6121 gaaagttctc cgtctaagaa cacatcccgg cgaaaagaag gaattccttt ctgggaagct
     6181 aaacgataag ccagagaatt agaagaagtt aagctgtcca gaaaatatac tccctttcct
     6241 tttaagctat cggtaaaaca actcatcagt aaataatcag aagtagcccg agaaccttta
     6301 tgattattta cccctttagc ctggggaatt agtcgcaaag cctcctccac ttttctttgg
     6361 actttctctt cactatctcc tacacgaagc aaaaaagctt ctccactgtt ctctcgggca
     6421 tcttctgcct ccataggaaa gtggataagc acttcttttc ctttctcctg agctaaagag
     6481 gcaatttttt tagccaaagg aagatgaggg aaaatagaaa cattcaattt cactggcagg
     6541 tctaaaaaac gttctgccat ctccagatta taccccaaat catcgataac cagcgctact
     6601 cgagctcggg gaggaataaa agtttccaga aaaaaccagg gttctgttcc tcgaaaaata
     6661 gaataaaaat tcttctctcc atcttcttct tttaccacaa aaccaaaaga agaaaccaac
     6721 cggaataaat ccaccatctt ctgagaaaat tcctctcgcg attcttggct atccagagta
     6781 acttgaactt cccaagctcc tccctcttcc ggggttttaa ctacctcgag gggagcatct
     6841 ttttttattt cctcaagaag gaaaagagcc agaaatttct ctttattttg aggatagaat
     6901 ttagccagag tcaagtaatg gttatttact tcttcttttt gccattctcc ccacaaaaga
     6961 aataaactcc aggccaataa aaaaaggata atccacagag tgggcttacg aaaccagggt
     7021 ttcctcatcg attaatttac gtaactcttc ctccgctttt ttaatctgtt cttcctcctc
     7081 actcactaca atatctggtt ctaaaccctt accattcaaa tctgttccat cgggaagacg
     7141 atatttagaa acagtaaaaa ttacccctcc cccatgagaa agggggaaaa tttcctgtat
     7201 tactcctttg ccaaaagttg tctctcccac caattccgca cctaaatgaa cacgcaaaac
     7261 tccagccagt atttctgcac cacttgctgt tcctttattt attactaaaa ccaccggacc
     7321 tctccatacc gggtctacaa aattgtagat actttctctt tttccctctc tatcttctac
     7381 aatcaaaaga ggactacctc gaggaacaaa catacctgaa gttaaaatcg cgctctgcag
     7441 aagtccaccg gggttattac gcaaatccaa aattagacct tttgctcctt gagcttgcaa
     7501 atcactcaag gcttttttta attcttcctg agtccgacca tgaaactcag aaatacgaat
     7561 aagtccaata tccccggaga gtatttcata ctctacactt tgaattttga ttatatctct
     7621 caccacttcc actacaatat tctcttcttc tccttctctc tgaatagtca aagtcaccga
     7681 agttccccgc tcccctctca ttcttcgtac tgcttcatct gaagacatac ccagagctga
     7741 catcccatct atttctacaa tgatatctcc agctttaatt cccgccttat ccgccggaga
     7801 gccttgaaaa ggagaaataa cagtaagttt gttatcctta atagctatga ctatgcccag
     7861 gccagaaaac tctccctcta cccggtcaga attttccatt ttataatctt cctcattcag
     7921 atagcgagca taagggtcac cggttgcctt taataccccc cggatagatt cttccatcaa
     7981 gaccttttct tcaagaggca catcagaata aaaattatca tgtagcagga gaagggtttc
     8041 aaaaaaaggt tgccactctt tcaggtttat gtcttcccct aaaccataac cccacttgct
     8101 ttcctgattt tcccagacaa acgctattac cagtaaaagt aaaacagcta cgcactccaa
     8161 taaccagatt tttttacttt tcactctcca ctctcctact tcagccattt gagggggtct
     8221 tcttctcgag cgctacttcc taccctcacc tcaaagtgaa gaagaggtct ctctgtaaga
     8281 ccggtatcac ctaccgtgcc aataacctct cctcctttta ctttttgtcc tccttccacc
     8341 tttatctccg agagatgggc atacatagtt atcacatctc catggtctaa aaggatggtt
     8401 ttcccataac cccggagagt ttgagccaga atcaccactc catcgtgagc agcaaaaacc
     8461 gggctacctt tctccgctcc tatatctatg cccggattat aaaaggaaat tccataacga
     8521 gggtcttttc gttccccgaa accttcaata attgtaccct gtactggcca gaaaagacgc
     8581 ccctgcttta ctggagctat agaaacagag ggctggcttt ctcttctctt ttttatatct
     8641 tcctgtagtt tggcaatcaa attttctaat tccttttgag ctttaaccag ttcattttta
     8701 cttctttcaa attgtttttt atccgaagcg attttctggc gcattttctc ccgggtgtct
     8761 tttagtttag ccaatttctg gttttctaaa agtgcttgct cttttaacac caactctaaa
     8821 cgaatcatct gcgctaattc tttctccttc tcctcttttg tccccttttc ttctaaataa
     8881 cgttcaagat tataattttg agattgatag tatttacgga ggagaaacca ctgttcttcc
     8941 cattgattag gatcactggt agtcataagt aattcccaga agccaactcc cccattatac
     9001 ttgtaagcct gaactaaaat attttttacc ttttctttgt cttcttctat cttttgagaa
     9061 agagttttaa tttccttctg caattctgcc ctctccgctt ccagctggag aatcttagcc
     9121 cgagactgag cagcattttt ctccaggctt tcaatttgtt tatccaaatt ctctatttct
     9181 ttaaccagat ttttttcatc tttcaataat ttatctaact cctggtctaa tttcaactgc
     9241 tcacttctca gtttctctaa ttttgtctgc tgctcagaaa tttcttcttc taagtcttga
     9301 gctcttcctg gaagattgag aaaaagtaaa ataagaaata cgagcaccca gcaagcaact
     9361 cttctcattt agcactttcc tcctccatcg agttgcaagc cagaattgcc cccaaaaagc
     9421 ctaaaaaact gctcatcaaa atactaacta ctcccagtgg taaaaccagt tcttcccagc
     9481 ttatccagaa aaagaaagga aaagaagtac gaagaaaatc aagtaagaag tgaaaaaaaa
     9541 ccaagctaac caccaaggct actgctcccc cacctaaacc ttccataatt ccctgataaa
     9601 taaaaggtgc tctaataaag cgtttagtag ctcccataag gttccagact tcaatttcta
     9661 tctgtcgagc ctgaatagaa gctttagtaa cggcagtaac cactaaaagg gaaaaaacaa
     9721 aaagagccag gagtaaaatt cctccggcaa tgaggaaaaa gtgataaaac cgcagaagag
     9781 cctcggtatt ctctccccca tagaaaactt cttcaaattc ttctatcttt tctagctcct
     9841 gagccagaag aggtaattct tctacccttc ttgctcgaac ttccagagaa gggggaaaag
     9901 ggttttcttc taaacccagg tctttttctt gaagattaaa gtaattcaaa aatctgcttt
     9961 tagcttcctc tgaactaatc agcctagtat ctcccacaag gggtagagtt tcaacctcct
    10021 ccttcacttt ttctatcacc tcaggagata aattttcccg gaaaaaagcc tgtacagtaa
    10081 agctgctttg ccacactccc cccatttgct ccaattccag atagccaaaa atgaaaagat
    10141 ttaaaagcag ctgagtaaag aaaacactta aaaaccccat cactaaatag gaagttcctc
    10201 gatgatagaa agcctctttg cttttagaca aaccctttct cctcaagatt gaattatttt
    10261 acctttatct aatactacta aacgagctgg aaaacgattt actaagtctt cgctgtgagt
    10321 agccacaatc actgtagctt cagacttttt agaaaactca aacaatgttc ctaccacctc
    10381 caaagcagtt gcgctatcca gatttccggt gggctcatca gctaaaacta aataagggcg
    10441 atgaattaaa gctctaccca ggcataattt ctgcttttct ccccccgaaa gccactctgt
    10501 tttaagatat tttttatctt gcagatgcac taattccaga atttcacccg ccagacggtg
    10561 agcatatctt cttttcaccc ctaagactaa aagtggggaa atgagattct cataaactgt
    10621 ccagtgaggc accaatttta aatcctggaa aaccactccc agattgcggc gcaaaacagg
    10681 tagataccgc tcctccagag agctaatttt acaccgctcc agccacactt cccctgaagt
    10741 tggtagttcc tccctggtga tgagtttcat caaggtagtt tttcccgctc ccgtagctcc
    10801 ccagaggaaa acaaattccc ctttttccaa ggaaagattt acattatcta aagctttgac
    10861 ccctacagga taaactttat agactctttt aaattccagg aatcccattt tgcttcctct
    10921 tattcggtat tataccatct ggtttctctt gtctttccat accacccgag caaaattaaa
    10981 gaaagcggga atgactcttc gcagacgccg aggctcacaa ataacacgat aggcccactc
    11041 cagattcaat ttcctcatcc actgaggagc tctcttttta gtactgctcc acacatcaaa
    11101 acttccaccc actcctatca ttaccagagc atttattctc tcccgatttt tttgaatcca
    11161 gtattcctgg cgaggagaac ccagacccac aaagagtata tgaggattgg ctcgattaat
    11221 atcctctata atttctccat catcctcaaa atacccatga tgaaaaccac taacttcaag
    11281 atgaggatgc cttttcttca gctcgttcac tgccttctct actacttctt ctcttccccc
    11341 taaaaaataa aaccgccaac ctttttcctc tcccctcctc ataagagctt ctcctatctc
    11401 taccccaggt aaacgatggt ttattgcctc tcccagaaaa cgggaagcca tcaccactcc
    11461 tatcccatca gctaccacaa tttctgcttc ttttaacact tcactcaaac cctcttcctg
    11521 aaacccccga gccaaaattt ccggattagc agttactaca tgcacttttt cttttttttc
    11581 tattttatct tctaacactt ctaaaagctc agacaaagaa caatcgtaaa aagaaaagcc
    11641 gaaaagctct cgggtaacgg ttttcatttc atcttctcag cctttcttaa aaattcctcc
    11701 agtattttca cgctctcctc tgatttttgc cctaactccc tctttatttc ttttcccttt
    11761 tctctcaaag tatctctttt ctgataaacc tcttccagtt tattttcttc caggtcagaa
    11821 aaagaaagaa ggttttcccc tttccaccac ttcgaaaaag ccctgatttt agggtctaaa
    11881 tccagagcaa accaggggat atccagcaaa gtagccagaa ttgcaggatg taaacggaga
    11941 gtaaaaattg tttttagttt ttcaaaataa ggaattattt gagagcattg agacataata
    12001 acccaagaac aatttaggga tttagccact tcttcagcta tttctccatc tttttcggga
    12061 tgaaaagcta ctacctccag attctctcct tctacctcac tgaattgtaa aaaagcccgg
    12121 cataaagctt cctttttctc ttctggaaaa gaacgaataa ataaagctat tttttccccc
    12181 ttaccttttt tctcttttcc cttccaggaa aaaaggggat cagctcccag aaaaggagag
    12241 gcagatccca actctaaagc gagagaaaga ctctcttcat cacgaaaaat ggaaagagag
    12301 ctctttttta gaaggtgaga gaccagaaac cgactttctc tccggataag aggtcccaaa
    12361 cctacccgat aaaaaataac ttttttattc caccactgtg cccagcgcaa aagagaaaaa
    12421 taataaagaa gagagcgaaa gctggtaacg tcctgcagga ggcttcctcc tcccaccagg
    12481 atataatcac tattttttat tacctgaaga atggaaaaaa aattgtctct cttcaccgga
    12541 tgtgtattct cgggaagagg aagaagaaaa ttactctccc ggtacaaaac ttgataaaga
    12601 agagaaatgc ttaattcttc tccaattctc tctaaatctt ctaaaactgc tcggaggctg
    12661 agttcgtctc cccagtttcc aaaaccataa taccctaaaa taaagaactt aattttctgc
    12721 tgaatgactt ctctctcccc atctccccac tctctgccac aaaaagagag ctaattttat
    12781 cgctataatg taaattactg ctacaggaag actcagcacc aaagcattaa gaaatcgcaa
    12841 aaggatgtaa agaaaggggg tatgaatgtg acagaaagaa ttgaaaaagg tggcaaatcc
    12901 tactgctact cccaaccata gaattagact gtaggaggga cggactttat ctttgttcca
    12961 atagaataaa agccagagag caggataacc aatcaaaaac tctttagtac gaggtcgcat
    13021 aaacaaaagg cgctctaaga gagctcgaat cctgatttct aatccaccag caggcagaag
    13081 aggaaagttg cccgagcgag taaggtaaag tagcgctgct actcctaaaa atgccaccac
    13141 caatagctca aatcttttta aatcttccag caaaacctgt cggatactca aaccccaata
    13201 accttttcta aaaagataaa ggagggtcaa aagaggaggg acaaggagag aaactttaac
    13261 tccccaatat tggtaaattc gcaaaacaaa taaccaatta taaaggccta aagagacagt
    13321 taaagctcca gaaaagaggg ttataaacgc ccaccctaaa ccctggagtt ttcccttccc
    13381 tgaaaaaata gtgtcaatca aaataattac tcccaaagtg ggataaataa taccggcgag
    13441 aagtcccaag accctcattc cccacagagg attatagaaa attaatcctg caagaggaat
    13501 aaaacacaag gggataaccc agaaagaaga acggaaaaag gaaccccaga aataaaaagc
    13561 aaaaaaagat aacgctagcg aaaagaaaat caaagagtaa agaggagggg aaaaagaagg
    13621 gaaaggaaca ctcacttttc ctacagtata cccattatcg gttaacgctt ttttaaccga
    13681 gaccaaataa gataaatttt tgtcccagtt tccaaaacca ggagggtcgg taaaaagacg
    13741 gagatagagg atgggaaccg acctttcttt tactgcccgc aagaatcggt ctcgagcttc
    13801 caccggggtg taacctttca gctcttcagg aggaatgcta tgagctcgca gggtaaaagg
    13861 agagacaaga gaagcaaggg tgctttctcc tttttgcccc acaaattcaa gatatcccca
    13921 aaatagccct ttttcctcta aaaaagaaga gagaagacgc agcgaatcca agcgaggagc
    13981 gcccagaaca ctatctccag aaaaaataac tccttcggct acttcccaga ctttctcctg
    14041 gagcaaattt tctaaaaccg aaggaggaat attataacga ttgtaaggac gaagtataag
    14101 atgaaaccct tgttcttgta aggattgcaa aactctataa tcccagccta ctcctatttt
    14161 atcttcttct tcatagagaa tatttacccc taatatttct cctttttctt ctacttcttt
    14221 ccctaaaaga agaaggtgat tttttaccgt ttcccgcaaa agaggatcag aaatctgaaa
    14281 ataacgtaaa agaggagaag aaaaaggaga aggagctact gaaaccgccc tcccctggct
    14341 gactaattgc ctccaggtga gttcgctaat tcctaaagta tcaaaccctt cttttttcag
    14401 ttgttcagca agaagtaaag ggtctgtgcc ttctatcttt cctacccact ctatatcatt
    14461 ccagtctact accaactcta ccactttaga ttgagactct ctttcccatc tctcagaaag
    14521 aatggggaga gagaaaaaga aaatgattaa aaaccctacc agcaaaaaga tatttctcct
    14581 catctttcct cctcaatttc taaaagagga tctccactat ccacggtgtc attttcctcc
    14641 acgtagacct cttttaccac tccccctcgc tctacagcga tctcgttttc cattttcata
    14701 gcttccagaa tgattaccac ctgtcctgtt tccacttttt ctccctttgc tacattaata
    14761 gctaaaaccc ttcccggcat gggagcccga agagtggtag taacctggag agtagaagat
    14821 gaaggaacta ctttctttat atttcttata ttccaatgat tctcctctac ctctctaact
    14881 tctgctacct caacttcata ttcttctcca ttaactcgca ccaaaaattt cctcatcgct
    14941 tacctcctca atcgaagtac ccagcaccgc tcgtgtttac ctaattcctt ccagttttta
    15001 cttttcttcg gtacataaga tgaagaaggc ttattttctt catccaaaat ttggaaaata
    15061 gcagcaacaa tagctgccac tacttggggg ttcaagccca cacctaaatc atactgggaa
    15121 attaccatgt ttctttccct tcccttcctc tcgtttagac cagaaaactt ccagagcttt
    15181 agctatttta atccgggttt ctcggggatc gatgacttcg tccacataac ctctcctggc
    15241 tgcctgataa ggattataaa actcctggcg atattgttcc agtagctttt ccctctctgc
    15301 tttagaatct agagcttttt ctatttcctg acgaaaaata atgtctaccg ctccttctgc
    15361 tcccataaca gcaatctcag cagtaggcca ggctaaaacc agatccgccc ccaggtctct
    15421 actgcacata gctaaataag ctcctccata agctttccgt aaaatcaccg ttattttagg
    15481 aacagtggct tctgaataag catagagcat tttagctcca tggcgaataa tcccccccaa
    15541 ctcctggtta ctacccggta aaaagccagg aacatcaact aaagttaaaa ggggaatgtt
    15601 aaaacaatca caaaaacgga taaaccgaga agctttatcc gcagcatcaa tatccagaca
    15661 ccctgccaaa tgataaggct ggttagccac tacccctacc acttgtcctc ctattctgga
    15721 gaaccccact attatattac gagcaaagtc tttttgcacc tctaaaaaac tatctttatc
    15781 cactaattct tctattacct ctttcatatc atagggttta ttgggatggg gagagactat
    15841 ttctaaaaga gggctattat ccctatccca ggggtcagaa gaatctttta ccggaggttc
    15901 ttctacatta tttgaaggaa gataactcaa aagagtacgt acttctaaaa agcactcttc
    15961 ttcagtagaa gccacaaagt gagctactcc actttttgtg gcgtgtgtcg ctgctccgcc
    16021 cagttcttca gctgaaatct cttcaccggt ggctgccttt actacctgag gtccggtaac
    16081 gaacatcttg ctctcccctt ccaccataaa aataaaatcc ataagcgcgg gagaataaac
    16141 tgcccctcca gcacaaggtc ctaagataag agcaatctgg ggaataacac cagaagcctg
    16201 ggtattgcga aagaaaatcc ccccatagcc agaaagagaa tctatgcctt cctggatgcg
    16261 agcacctcct gaatcattta ttcctataag aggcgcaccc actttaaggg ccatatccat
    16321 aattttggta attttccggg cgtgcatctc tcctaaggaa ccacccatca cagtaaaatc
    16381 ttgagaatag acaaacacta ctctcccttc tattgttcca aaaccggtta tcactccatc
    16441 agcaggaagc tttctttctg ccataccaaa ccggtcggct cggtgctcca caaaaaggtc
    16501 aatttcctca aagctcccct catccaccaa cagttctatt ctctctcgag cggtgagctt
    16561 ccccgcctcg tgttgctttt ttagggcctt ttctcctccc ccctggagaa ggagagcttt
    16621 ttcttttttt aattcttcca ctaactctaa caaggtcttt ttaggcataa gtcaactttt
    16681 cctctctatc cgctattcct tctacattga cgttcacagc tctcacatcc accccggtca
    16741 aatattccaa tccttctttt aatctctttt gagttaagtg tcccgcttga gaaagtgaaa
    16801 aaccagaatt aataacacaa ttcaccacta tctctacccc tccctcaaac tggcgaatag
    16861 atagcccttt tatttcctct atccagggaa aacgagaaag gaaaaaattc aggagagaaa
    16921 gaagagcttt ttcagaaacc actaatttac ccagataact aaaaggaggt cgcactactg
    16981 ttttctctcg tgaagaataa aaccacctcc agatggtaac tggcacagta tcgaccaggc
    17041 tattccacaa gtttctcttc aactctacaa gaggcacggg aatagtatgc tttccctcat
    17101 tttcccgagc tgatagtgct aactctatct cctgaggaga agctattttc tcaatctcta
    17161 aaatttcttc aggaggaggg agttccagat gctcacaaat tttctccacc ataggaagag
    17221 agactcccag tatcattatg ttttgaggag caagtttctt cagagctctt ttaactgcct
    17281 gccggtcttg agaaaaatag aaaagggcta tcttagttgc acgaatttta ttaatctctg
    17341 ctttagcaga gcgaccagca ataatcctcc cttccgaaat aagaagccca tcatcaatta
    17401 tacaatcaat tttgtatttc tgagctaaat gaggaacatg ataactcttt cccgttccac
    17461 tcggtccaat caaagtgtaa actttcatgg atgttacccc ttttcgggag aaatttgcct
    17521 cttctcaaga aaaacctcta taaaatcgtc aatttcccca ttcatcactc gctcaacgtt
    17581 gcctgtttcg tgaccggtac gatggtcttt caccaggcta taaggatgga aaacgtaagt
    17641 gcgtatttga ttaccccagg caatgtcttt acgctctcct ttaatctctt gaattttttt
    17701 tctttgttcc tcttggtaat attcataaag tcgagacttc aatattctca tagccaaagc
    17761 tttattctga tgctgagagc gttcattctg acattgtact actatgccgg taggtatgtg
    17821 aataattcgg accgctgaat cagttacgtt tacatgctgg cctccatgac ctccagcacg
    17881 aaaagtctct atcttcaaat cttgaggatc aatttccact ttaatatcat ctcccatctc
    17941 aggagtaact tctaccaaag caaaggtagt atgacgacga tggttggcat caaaagggga
    18001 aagacggata agacgatgca cccctttttc tgatttgagg tagccatagg catattctcc
    18061 ctgcaccagg aaagtcacat attttaaacc tgcttcctcg ccttctaaaa tatcagttac
    18121 tcgaacctca aaaccctttc tttcacaaaa acggagataa aggcgaaaaa gcatttccac
    18181 ccaatcttga gattctactc ctcctgcccc ggaatggatg gtaactatgg cattacgact
    18241 atcttccacc tcagaaaaaa gaagctgaat ttttaagtct tctgcttctt gaaccagact
    18301 tctaatttcc tttctcagct cggaaaattc tgggtccgct tcattaatca gggaactgaa
    18361 ttcttgcgct tccctcaacc gctcttgtaa attctgatat cgctcccaga tctggcgata
    18421 agatttgtat tctcccagaa tctggttttc tccatttcta ttatcccaga aaccttcctg
    18481 agacatcttt ttttctattt cttttatttt ctcttctaac tttaactggt caaagataac
    18541 cccctacttc ttccaattta ctcttaactt cttctatttc ttctattaca tccggcatct
    18601 tttctctcct ttcattcatt cttcccacag caatatttgt attttaaacc actcccgcaa
    18661 gggcaggggt catttcttcc caccttattt ttacttcgta cagtggtcac tttttcccga
    18721 ggagccctgg aaggggaaac cctgggtgac gactttttag cttcagcaac cacttgtaga
    18781 tgcaaaagat aacgaatggt atcttctcga atatgagcta tcatatcctc aaacatatca
    18841 aaagcttcca gttgatattc aacaaagggg tctcgttgtc ctaccgctcg caatccaatc
    18901 ccttcccgga gatggtccat attccgcagg tggtctttcc agtagttatc taccactcgc
    18961 agaagcacat acctctctaa atcctggaat agaggctccc ctatttcttg cactcttttt
    19021 tcataagctt ctcgtaaaga ctgcaaaata tcttcttgta aaagagatgg agaatagttg
    19081 agtttctttt cttcatttac tctccactcc catcctaaaa cagaaaacaa gcgctggctc
    19141 aacccttccc aatcccattc ttcaggataa accttctccg gagcataggc agaaactata
    19201 tctttaatca cttcttccat cattcccaga atataaggac gaagattttt ctctcgaagt
    19261 actgttcttc tctgtccgta gattacctct ctctgcttac tcatcacatc atcatactgg
    19321 aggagagttt ttctaatctg gaagtaatgt ccttctactc ttttctgagc tgattctata
    19381 gctcgagtaa ctaaagggtg ctcgagagga actccttcct ccattcctaa tttttccatg
    19441 agttgagata ttctatctga accaaaaaga cgcaacaagt catcttctaa agaaaggaaa
    19501 aactgcgatg agccaggatc gccctgcctc cctgctcgcc ctcgcaattg attatcgatt
    19561 cttctacttt catgtcgctc tgtgccaata acatgcaaac caccggcggc tttaacttct
    19621 tctccctccc gctcagtcgt ttctttgaac tgagaaagaa gctcccggta aagctttcga
    19681 gcttctacta cttctggtaa attagtaggt aaaccttctg ctgcctcaag aattatttct
    19741 tctttaaatc ctctatttct catttcctcc cgggccagaa aatcggggtt acctcccaaa
    19801 agaatatctg ttcctcgccc agccatatta gtggaaatag taactgctcc ttttttgcct
    19861 gcctgggcaa caatttctgc ttctttttca tgatgtttag cattaagcac ctggtgaggg
    19921 attttccgat gatgaagaag cttactcagc ttttctgatt tttcaataga aaccgtgcct
    19981 actaaaaccg gccttcctgc cttatgctgt ttttcaattt cttcgactac tgctgcaaat
    20041 ttctcttctt ccttacggta aataacatct ggatgattag tccggcgaag aggtcgatta
    20101 ggagggataa ctaccactgg cataccataa gtgtaaatga attcactctc ttcggttttt
    20161 gctgttcccg tcattccaca ggttttgtta tacattcgaa aataattctg gtaagtaatg
    20221 gaagctaagg tttgattctc attagctact ctcaagcctt ccttggcctc tatggcttga
    20281 tgaagaccat cgctgtaacg acgcccatgc atgagccgac cagtaaactc atctacaatg
    20341 atcacttccc catcttttac tacataatcg acatcttttt tataaaggtg atgagctcgc
    20401 agggcttgtc taaccagatg ttccaatgaa tcctttccgg tctcctcgta gaggttatcg
    20461 atatgaagca ggttttccac cttggtgact cccctttccg tcaaccatat ggaacgggtc
    20521 ttttcttcat aatcaaagtc ttcaggatag gatagttttc tgactacttt atctactttg
    20581 tagtaaattt ctgtgttttc ctctgctggt ccggaaataa ttaaaggagt acgcgcctca
    20641 tcaatcaaaa tgctatccac ttcgtcaaca atggcataat ttagctctct ctgtaccaca
    20701 tcttcaaagc ggataaccat attatcccgc agataatcaa aaccaaactc ggtattagtt
    20761 ccataagtga tatcgcagag ataggctttc ttccgctctg caggagaaga atcatgctga
    20821 atcaagccca ctgataaccc caaaaattca aagatcggcc ccatccagta acggtctctc
    20881 ttagctaaat aatcattaac tgtaacaata tgtacccctt ttccttctaa ggcgttgaga
    20941 taagcaggca tagtggctac cagggttttc ccttcaccgg tggccatttc agcaattttt
    21001 ccctgatgaa ggactacgcc tcccataact tgtacatcaa aaggacgcat accagttatt
    21061 cttcttgctg cctctcgtac cacagcaaaa gcttccacca ataaatcatc taaactactt
    21121 ccttcctgaa ttcttctttt gaattcttca gtcttttgcc gtagttcttc atccgaaaga
    21181 tgagaaactt ctttctcccg agcattaact gcctctacat atttatcttg caattctcta
    21241 atttgctttg cctgagctct atcagcccaa aaatctttaa tccagctcat tattttcctc
    21301 ctgaatatac ctctaaatta taacataact atagggggga aatgccccct atgacctcct
    21361 gcaaaaatcg aagaaaaaca aaaaaattac tgaggaagat agaatattcc tcagtaaaag
    21421 tatttagatt cccgattgaa gatctcagga atgacaaaag gaggtctatc attcctgagt
    21481 gtatttatca gttgctcgcc gtcattcctg aatgtattta tcaggaatct acattattta
    21541 aggacgaagg cctttagatt cccgattaaa gcgctcggga atgacgaaga gagggaattt
    21601 tccttcctct aatgcttttg tgatcaatca ttatttttcg tcctcagctg ggaactcaga
    21661 gccatccaat gttctaccat taagtcttct gcccgccact caggattaat acgagcttga
    21721 gccaaaattt ctttagctcc ctcttttccc aatttaggac gaaggagctt ttctattttc
    21781 ttccgccgct gagaaaataa aaaatgaacc cagcgcataa tttccttata agtatcaggc
    21841 tctatctggg aagaaagagg agtaaattta agaatcagag aatgaactgc aggaggagga
    21901 gaaaaagaac caggagaaac ctcctttatc ttttctactt tatagagaag attaactccc
    21961 accgaaagag gacttccttt ttcctgtaat attttttccc caaattctcg ctgaaccatt
    22021 aaaatcaaaa gattccacct gatgttactt tctagaattt tagaaaaaaa aggagaagaa
    22081 atagcataag gaatattaga aaccaatttg aggggggaaa aaggagaaag aagagatggt
    22141 aaatcagctg ataaaaaatc ttctcgataa agaactaaat tagaataact ttttaacttt
    22201 tcctgaacca tccggtagag ttgctcatca atttcccagc ctatcactct accagctttc
    22261 tgtaccaaga tttcagtaag gacagcctcc cctactccca cctctactac tgtatcttct
    22321 ttttctaact cggcttcatc aattattatg tctcgaacag ctttatcaat caagaaatgc
    22381 tgtccccatc ggcgagagtg atggaaacta ctcaaactta cctccttaaa ctacaaaact
    22441 atcaggctaa gggtttaaca tagaataatt ctccttcgga tgttccaggt gtccagggaa
    22501 aaaataatca cagcgatcca ctggctcttg tctttctttt tgctctccca ataagcactt
    22561 ataaatcccc ttctttttca tttccggatc gcaatgcctg cacacctggc ataaaccttc
    22621 aggattgtac aattcaaatc acctcttttt attttattct aaaacccact ctacctcagc
    22681 aacctctata ttcacccccc tcaaagggaa ttcccactct cttattgcat tagccactcg
    22741 tttctgcact tcctgtgcca cttccctcca gtttgcccga gaatccaatt ttaaagagat
    22801 attaataaat acctcttctt tagtcgaaac tctatcgtta ggagagagag cttctcctcg
    22861 caccatgact ttagtattct ttaagagata gtcaaagtct cccttcaccc cctctaccgg
    22921 gaaagcactg cgtgccgcca ttttcaccag tgtagataca ctcactttta tttctcctgg
    22981 ctcttcgcta aaaggcatta tttctctcca gtttcttcca aacataattt agcgccgcct
    23041 cagcattttg aaattgtggg aaaaaatctc cttctcctgt agaatcctgg attttccagg
    23101 tattcaatcc agcaaggact ttaccatggg aaagagcaaa ggcaatttca cttaaagttc
    23161 caaattggcc tccacaggct atgagagccg aagcagtaag gacaattatc aaattacgga
    23221 cttctcccaa accggttaca atttttaaat ccacataggg attagcatct ccctcaaaat
    23281 taccaggtaa aacccctatg gtaaaaccac cagcttcttt tgctcctcga caagccgctt
    23341 ccatcactcc tcccaatcct ccacaaagga gaatagcgcc tcgctcggca ataccttttc
    23401 ctacctgata agcaatctca tatagttgcg catcacagga atgttgacca ataactccta
    23461 ttatcaagtt acgattcata gcccagatat ttttttaagt cttccggctt taaattatct
    23521 aaaaattgac gaaactcctc tatctcctta tcgtctatct ctcctgtcgc cacagtagta
    23581 gagctgagaa cctcctcaga agcaaagatg ggagctccca ttcgtaaaga aagcgctata
    23641 ccatcactgg gacgagcatc cacagttacc tcgtttccct ctacctggat atatagaagg
    23701 gcgtaaaaag tgttatccac aattttagta atgactattt tttccagctc tccccctaac
    23761 tgatggataa gagaagctat aaggtcatga gtaagaggac ggggagtttt aactccctgc
    23821 aaacccaaaa gaatagcctg tgcttcaaat aaaccaatcc agataggaag gctccttttt
    23881 tcttcttcat cgctcaggac tactactgcc atggcgtttt tctggtcaaa catcaaaccc
    23941 tgaactttca ttctgatcat aactaaaaat cctttctatc ttacattcta ttattataac
    24001 gtaaattaag tttccttcca aaaatcgagt aaaaattctc ccatactttc tctctcaaag
    24061 ttaaaagaga aataccccat aattgagcta gaaacgaagc agtttcaatt aaaaaagctg
    24121 gttcgtttct cttgcctctc cgcgattgag gaggaaggta aggagaatca gtctctaaaa
    24181 gaacccgaga gaggggaatt tttttaacca cttctcgaag atgagtagcc ttagggtaag
    24241 tcactactcc ccctaaggaa aaataaaccg gttgaagcag cgcttcttgc aaaatctctt
    24301 cgtctcctga aaaacaatgc cagactacca ccacttctcg caccctctcc tttataatct
    24361 gccacaaatc tctattagat tcccgagagt ggacaattaa aggaagagaa aacttttctg
    24421 ctaattcaat ttgctggata aaagcctctt tttgtttctt gatatgactc ctatcccagt
    24481 aataatccaa tcctacctct cctatagcta gaaccttttc cttttgtaca tattcctcga
    24541 ggatagagaa gtcgggcaat tcatccccta tctcctgagg atgaataccc acactggcat
    24601 aaagcgaggg ataacgctct actaacttta aagtttcctg aaccgaaggg agggaggtag
    24661 cagcattgac tataatttcc actccagcgc tttgcgctct atttataacc tcttctcgat
    24721 cttttagaaa ctcctcccct tctaaatggg catggacatc cacccaaaag ctcatcttac
    24781 tctgcttccc ggtgctatat ctcgatccac agttaaaata ctaaccattc cttttccatc
    24841 atcagcagct aaaagcatcc cctctgattt aatcccccgg atattagctg gttttaaatt
    24901 agtgaggacg atgattttct tccccatcaa ttcttcaggg gtgtaataat tagctaaacc
    24961 tgccaccagg gttctttctt cttctcccag attaactcgc aaagctaaaa gagctttagt
    25021 tccttctatt ctttctgcct ttactatctc tcccacccgc aagtcgatat tttgaaaatc
    25081 ttccagatta atttctggca acaccatctt tttcactcct ttattctata attctcggaa
    25141 acaaaggttg agcttctccc actgaataaa cttctgggcc ttctctccag tacagggctt
    25201 cttccaaatt ttcttttctc aggtcccggg gcaaacctaa aagctctctc aacttcaaag
    25261 cagtatcagg cataaaagga taaaggaaaa aactcagtaa tcgagcatta tctaataatc
    25321 gataaaggag attctctacc tctttttgtt ttccttcttt gtgtaacttc cagggagctt
    25381 ctcggtcaat atagcggtta gcaaaatgca ccagtttcca aacctgctcc aaagcctgcg
    25441 caaaagcaaa ctgctccata tgatgagaaa aaccctcttt cacctctttc agcaactttt
    25501 cccattctcc atcttgataa ggagaaggtc tccttactac tcctttttta tatctccata
    25561 ccattcctaa agttcgatga ataaaattac caaagttatc ggataagtca gagttagaac
    25621 gttctatcag tgctttttcc gaaaaatctc catccaaacc aaagctcact tccctcaaga
    25681 gaaagtatcg gaaacggtct accccataac ggtcaatcat taaataaggg tctactacat
    25741 taccccgaga tttggacatt ttttctcctt ctaccgtcca ccaaccgtgg gcaaatattc
    25801 tccgaggtaa aggtaaagat gcagccataa gcatcccggg ccagagaaca gcgtgaaagc
    25861 gaaggatatc tttacctact aaatgatagg tagaaggcca aaaagtagaa aatttttctt
    25921 ctgaacttcc ccaccctgct acagttaaat aattaaccaa agcatcaaac cacacataaa
    25981 gagcatgacg agggttagag ggaacaggta tcccccattc cagccccagt cgagagacac
    26041 tctggtcttt aaggcctcct cttataaaac tctcaatctc atgataccga ctttcaggca
    26101 ttataaaatc gggatgactc tccaaatatt ctagaagtgg ttcctgaaaa cgagaaagag
    26161 cgaaaaaata actctcttct ttcaatcttt ctaaaggcct tccgcaatcc ggacaaatca
    26221 ggcgttcgtc taattggttc tccggccaga aagtctcaca gggaacacaa taccacccct
    26281 cataatctcc cagataaatg tagcctcgct catccagtaa ccggaaaaaa tcctgcacca
    26341 cttttatgtg gcggggatca gtggtgcgga taaactcatc gtaagaaata ttcattttct
    26401 cccacaagtc ctgaaaacga accaccactt tatccactaa ttcttgagga gaaatcccct
    26461 gatctttagc agctttttct attttttgtc cgtgttcatc ggtaccggta gaaaaaagga
    26521 ctcgataccc ccgtaagcga taatagcgag ccagaacatc agcagcgcaa gtagtatagg
    26581 catgaccgat atgaggaaca tcatttacat agtaaatagg agtggtaatg taaaaactat
    26641 tatccatctt tcacctcttc aattaaatca aaaatttgcc agataaggct cttctttttc
    26701 tatctcttcc cagaattctt aattaaactt acaacccggg tcaggagcta aataatcctt
    26761 aaaataagca taagtccgaa cccggcaacc tccgcagacc aaccgataat cacagttctc
    26821 gcaatgtccc ccataaagtg ttctatcacc caaatctttt aactccaccg attccttcca
    26881 gatctgggag aaagactgtt ctatgatatt tcctgaagca ataggcataa atacacaggg
    26941 ggtaatcact ccatcaggtt gcagagcaca ataagctctt ccggccccac atcctcccac
    27001 atatttcgct gccactcgag ccagtttcct ctctgatacc gaataatgac cagtaatgac
    27061 cattcctccc ggattcttct ccaaacattt cctaccaaat tgaggggtag tactgaaaac
    27121 aaatatcctt ttttccatta aacactgata gagaaattct aacacttctt ctcgctcccg
    27181 gggagagagg tcttcagcca aaattacttt tgcccttact gtaatgggta agattttact
    27241 ccaatcttac ttctccatct tctgtcggct cactaacccg gcgaaatatc ccccgaatta
    27301 acctggagtt gagatatctc cttatccttt ctggattcat aaaatgacta tccatctccc
    27361 ccacctcttt gacttatctt atcctatttt atacctattt tatcaagaaa aaaacaaaag
    27421 aactcttttt tgtaaagata gaggaatttt atcggttctc tttttcctga actttatttg
    27481 taattcttga cgtaaaagtg ggacagctta acggcccctc ttttctactt tccccgaaga
    27541 tgagccactc caccctttaa ttttcagggt gacattatcg cagaataagc acattga
//
LOCUS       DAOSVE010000015        25956 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_1234_length_25956_cov_77.229489, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000015 DAOSVE010000000
VERSION     DAOSVE010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 25956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25956
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1234_length_25956_cov_77.229489"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="PK016_02375"
     rRNA            complement(<1..985)
                     /locus_tag="PK016_02375"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1783..2144
                     /locus_tag="PK016_02380"
                     /pseudo
     CDS             1783..2144
                     /locus_tag="PK016_02380"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2264..3013
                     /gene="mgtE"
                     /locus_tag="PK016_02385"
                     /pseudo
     CDS             2264..3013
                     /gene="mgtE"
                     /locus_tag="PK016_02385"
                     /inference="COORDINATES: protein motif:HMM:NF013895.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
     gene            3138..3608
                     /gene="rpiB"
                     /locus_tag="PK016_02390"
     CDS             3138..3608
                     /gene="rpiB"
                     /locus_tag="PK016_02390"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HXL01955.1"
                     /translation="MKIGIGSDHAGFAFKEAIKENLSKEGHEVIDAGAQDEQPVATAP
                     YAQAIAEGLLNGEFERGILICGTGGGMSVTANRYPGIRAVLCFNPFTARMARSHNNAN
                     ILVLGSRTITLPEALELVDIFLSTSFAGGKYAERLKYLEEIEKKVAKILQERFA"
     gene            3757..4842
                     /locus_tag="PK016_02395"
     CDS             3757..4842
                     /locus_tag="PK016_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HXL01956.1"
                     /translation="MLLKIMDRYLIKDSTSGMFTWVGAVTVIMLVQTLFELADFFVNK
                     KVPLLIVLEILLLYIPAHMVMTFPISGLLASELNLGRLSRDSELIALEASGVSLRRVI
                     LPYIIFSILVSVGSFTLNDLVVPETNHRAQVLIREYVYKEGPPQIEKNVFFRDSQNRY
                     FYVNELDNKTWEMKDVIVYEIGKNRSFPDVILAKSALWLEDRWLLKEGVVHRYNENGL
                     LIQEIEFSEMVIDMKEELKSFFTEQRSPEEMASRELKKQIDILKEAGAKTETFEVAYH
                     LKYSIPFSALVFILMGIPLGIQRTKDTRTIGVIVTVILAFAYYMLLSIFRSLGRGGIM
                     EPIIAAWMPNIIFGLPGFILYLTVDRK"
     gene            4851..5531
                     /locus_tag="PK016_02400"
     CDS             4851..5531
                     /locus_tag="PK016_02400"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXL01957.1"
                     /translation="MKKILFYLIISVLVISWGGGWLRAEENLFDQAMEARGKGEDEEA
                     LKIFERCVEEGIEVHQAYYEMGKILLDQKKYRRAIIVSEKAIEAYKEHLKNHPEDHNA
                     WWELGDIYGTRSNYNYSDEWKEAQEALEKAVELSPSNSFYLLYLGWIYYQEGDNEKAE
                     SIFRKILDKNPEDFQARRFLIDALIEEGRKKEAKEELAFILEKCPKEDELYSWAEKKW
                     EELEGENQ"
     gene            5528..6712
                     /locus_tag="PK016_02405"
     CDS             5528..6712
                     /locus_tag="PK016_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXL01958.1"
                     /translation="MKTKVIFLISLILVVGVFSGCFSVTQKGSLEGEVWGESGPLAGA
                     VVEGGGSTSITNEEGKFLIEGLPSGETYLFFSCLGYTGKAIKVEVKAGETGKINQEGR
                     IYLVPQDEESLKDYIFELYSLGFYERAISQVDLFLTDYPEGNSLPQVLFIKGASLYHL
                     KNFQDAISLLSHIRDNYPDNEFSDDAQYLLAKSFGEGLKNYENAIVEYQRLIDSYPDS
                     EFLGIAYYEMGDCYYILGSYSEAYLSYQKAQTYGGEVEKGALYSSAHCLYKLELYPQA
                     ALLFSEYVERYPQTDISDDAQYFAGAAWYKAEEYSQALVAFQHCVNNYPQGTWYNGIL
                     IAPAALLNQGLCLEKLGRYREAYNIYLQIIREYPGAKWADGSSLIKNVQFRIEWLENN
                     VL"
     gene            6724..7305
                     /locus_tag="PK016_02410"
     CDS             6724..7305
                     /locus_tag="PK016_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXL01959.1"
                     /translation="MHEEKSDRELITEVLNGNEDLFYFLVRRYEKQILNYIYRLVKQR
                     EEAEDLAQETFTKAFFALRQYDHTYEFSTWLFRIALNVCRDYFRRRKIAFFSLNTPVG
                     EEEETEWGELIEQNSFPDPDGELDNRELRRELEKAIDHLPLKFKEVIVLRHLEGLSYS
                     DISEITNLPAGTVKTYLHRARKKLQKELKKYLD"
     gene            complement(7375..7866)
                     /locus_tag="PK016_02415"
     CDS             complement(7375..7866)
                     /locus_tag="PK016_02415"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HXL01960.1"
                     /translation="MELVYRVLNRGTLLWILCLKPQLLFMRYNFNVLTEEGEKIIVTI
                     DKKELSLLIEELLEKLPPLFKNNISNVEFVVEDYPDSEIQGRFGSRFLLGLYQGIPLP
                     RRSGNYNLVMPDRIVIYRENLERIAGKNWPEELEKVLWHEIGHYFGLSDEELWKLSKQ
                     KEK"
     gene            complement(8149..8466)
                     /locus_tag="PK016_02420"
     CDS             complement(8149..8466)
                     /locus_tag="PK016_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01961.1"
                     /translation="MVDPVSMQNVVIRSPEVARIQKVEDNASTLSSYALAQEAQDESQ
                     KVEREVIPSPETDRYPKEEKKKEERDMIDTEKKEKKEKKEKKEKKQLDVKGDKGNIVD
                     VRV"
     gene            complement(8481..9896)
                     /locus_tag="PK016_02425"
     CDS             complement(8481..9896)
                     /locus_tag="PK016_02425"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HXL01962.1"
                     /translation="MLKSLKLLEEDNRIQLFISGDGLRSELVVKKPLSEEELSSYEEL
                     KDFLNSRGITVGIKEEVLERGKKLLQEEGRHLVAEGKRPQRGKDGEVKILFTQELEKK
                     LEEDESGRVDLFDFVKIPEVKEGDLLAELIPPGEGVPGQDIFGKEIPALLGRPAKIRV
                     GKNVELVEEGTKAVAKVAGRPVLIGKTISVLPLFEVDGDVNTSTGNISFIGSIVIRGT
                     IRSGFKVEAEGDIEVWGNVEAAIVQSQGNIMIRGGIFGREKGLVRARGDVKAKVIENA
                     TVEAGQDVIVGEAIFHSQISAGRKVVVGGRGLAVGGKIKAGNLVWVKTLGSSMGTITE
                     VMVGIDPTLREEYNFLMGELERVKKQIEEGEKVFRTISERQKVGKGLDPGMIKVLEKL
                     EKTCRLAREKKEFCEARLEELEEIISGGEGKVLVEEKVYPQVRITVGNLTYPVRDEIP
                     YASFYKKDQEVVLGSFEKPRS"
     gene            complement(9909..10670)
                     /locus_tag="PK016_02430"
     CDS             complement(9909..10670)
                     /locus_tag="PK016_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HXL01963.1"
                     /translation="MNLTPEIENIDKVWESYLKTRDSAEREKLAEHYLYLVKIALGRL
                     LYILPSYVDREDLESYGVIGLLQALDRYRPDKGLRFETFALSRIRGAVLDYLRSLDPL
                     TRRERRGWKEVMATYQELEKRKGREPTLSEIAKELDISVGEVTWIIERGKSAFFFSLE
                     EEKEEGKKLLDGVKEERLMFNPEALLENEELLDYLGKAIEELPERERLSITLYYFEGL
                     TLKEIGAILEVKESRVSQIISRALLKLKTKLSDWK"
     gene            complement(10648..10890)
                     /locus_tag="PK016_02435"
     CDS             complement(10648..10890)
                     /locus_tag="PK016_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01964.1"
                     /translation="MEVRQAKNLARLLALFLSWILLVLYFIWGFWRNLSFLTLVWRGL
                     LLFSVAYLIMFYYFLWIISSGGNKETEVKDESNSGD"
     gene            complement(10893..11369)
                     /locus_tag="PK016_02440"
     CDS             complement(10893..11369)
                     /locus_tag="PK016_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HXL01965.1"
                     /translation="MYNRVVIGMAEYWVSSNPEDVLCILGLGSCVGLCLYDPVKKIGG
                     MVHILLPESIPGQSNPFKFADAAVPALWGKLKNGGASPKNVFAKIAGGSKMFSGTDSL
                     FDIGKRNVEVVKAELQKLNIPLRADDTGGNRGRSIMFYLEDGRLEIKTIGQKPVVV"
     gene            complement(11371..11997)
                     /locus_tag="PK016_02445"
     CDS             complement(11371..11997)
                     /locus_tag="PK016_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HXL01966.1"
                     /translation="MEISFEKLNEIQLDALREVGNIGAGNAATALSKVVGKRVKMEVP
                     LVKILPLKDVPDWLGGPEKEVLGVYLTMSGAATGHILFVMTIEDALKIMHILLGDLTP
                     SREQVLEMDEIARSAMEEIGNILSSSYLSALADFTGLRLNHSVPALAIDMAGAIVDVI
                     LIEISKKGDYALLIETEFVEEENRIKGYFMLIPDTRSLEIILRSIGVI"
     gene            complement(12004..12792)
                     /locus_tag="PK016_02450"
     CDS             complement(12004..12792)
                     /locus_tag="PK016_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HXL01967.1"
                     /translation="MLKREDIQYLRDKVLEKTGIDLAYYKENQLQRRLPFIISRAGAQ
                     SVEEYVELLDKNPQVLEDFKNRFAINVSEFFRNPERYEDLKNKVLPKLMKEAGLILKI
                     WSAGCSVGSEPYSLAMVLEEMPHRKPYRIWATDIDEDALEKAKEGVYSEDFISNVPSV
                     YLEKYFQFQGEGKYAVVHQLKKRITFEKHDLLQDEVKETFALVVCRNVIIYFEDEAKK
                     IAFKKMSDALQKGGYLWVGSTERITDPSSLGLRYVLPFFYQKGG"
     gene            complement(12794..13852)
                     /locus_tag="PK016_02455"
     CDS             complement(12794..13852)
                     /locus_tag="PK016_02455"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HXL01968.1"
                     /translation="MKKIKVMIVDDSSFMRKIVSDLLNSDPEIEVIATARDGLDAWDK
                     LRSLDPDVILLDVEMPRLDGLGLLEKIFEERPRRVVMLSALTQEGSEITIQALDKGAL
                     DFIPKPSGSISLDIEKKKEEIIAKVKSASTIPLSQVGKFVTQGRERKKISVREKEGRE
                     CPRLVFIAASTGGPKVLHTLVNNLSPLKKSGMLIVQHMPSGFTKSLAERLNNSAPFPI
                     KEASNEDKLVTNCALLAPGGKHLIIEESKEVRLSDDPPVKGLKPCADITLLSAVKVFG
                     GENILAVVLTGMGKDALEGCRALKKNGGTVIAQDESTSLIFGMPRAVIEEKLSDRVLP
                     LQDIPSAILEWDSRGDGS"
     gene            complement(13852..14496)
                     /locus_tag="PK016_02460"
     CDS             complement(13852..14496)
                     /locus_tag="PK016_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXL01969.1"
                     /translation="MKISQVFRPNRRISLGMNRKNEKGEEVVFYYPSRIELVGEEELW
                     VAAPQEKGVLVPIGVGEKLFVYVIGENEIFHFEGEVKNRLRKENMAFLILSLPQEVKR
                     EQRRNYVRIDVAIPLLIRKDDRTFLGYTRNLSGGGMQASFSGERNFLGIGDEVVFSLS
                     LEDKSIIGRAKVVRKDDPSCYAFQFVEITDQDKEEIIHYIFKQQIEIRKKGLLI"
     gene            complement(14509..16560)
                     /gene="flhA"
                     /locus_tag="PK016_02465"
     CDS             complement(14509..16560)
                     /gene="flhA"
                     /locus_tag="PK016_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HXL01970.1"
                     /translation="MNEALTKTLEKSLKNSDFLFAFLFILIILMMVIPLPPTVVDFLL
                     SCNITLSVMILLTTTYITNPLQFSVFPSLLLFATLFRLALNVSTTRLILLQGYAGKLI
                     ATFGSFVVGGNYVVGFIIFLILVIIQFVVITNGAGRIAEVAARFTLDAMPGKQMSIDA
                     DLNAGLIDEVEARRRRREIERQADFYGAMDGASKFVRGDAIAGLIITLVNFLGGIIVG
                     SLQRGLSIQDALSAYALLTVGDGLVAQIPALLISTASGLIVTRAASEDNLGQEVTREL
                     IQVPRVILLSAILLLVLGLIPGLPKLPFFLLASAAFVFYYLQGKEEKKGEEPAVTLSE
                     KKEELPRSPEEILKLIEVDPIELEIGYGLVPLVDASAGGTLLERITQMRINMAREKGF
                     VVPPIRVRDDLRLRPNEYVIKIRGVEVGRGDIMPHYYLAISPRGEAATIQGIPTKEPA
                     FNLPAYWVNEVNKEEAEIAGFTLVDSESVLITHLSEVIKAHAPSLLTRQDVQMLIDQV
                     KKVNQAVVDELIPQAMSIGEVQKVLQNLLDEGIPIRDLSLILETLADYAPQAKNIEGL
                     TEMVRRKLGRSIVKQYLEEDGKLHILTLDTNLENLLQRALQGEETLEPDFFRSFLDRL
                     SESVERIVGLGHFPILATTGKIRRGLRDLVSGYLPQVVVLAFEEIPKDVEVKVEGMIR
                     M"
     gene            complement(16553..17632)
                     /gene="flhB"
                     /locus_tag="PK016_02470"
     CDS             complement(16553..17632)
                     /gene="flhB"
                     /locus_tag="PK016_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HXL01971.1"
                     /translation="MPPQQDKTEEPTPRRREELRQKGKVPISVDFTSGFSFIVLFLLA
                     TFIFPSLSREMMSLIEEIWAGNYYSREVDSQWVFHLAQIGVGYFARGVLPFVVVAAGL
                     ALFLGFLQTGFVISGEKLKPNFDYVNPVRGLERLFSLRLLVEALKISIKVFLGIVLAY
                     VVVKDALPRLTDLIYMELLAGIETVEEIAERLGLYLGGLFLGVGFFDFFYQRFEYERN
                     IKMTKEELKEEYRRTEGDPIIKSHIRRRQREIARRRMMQEIPKAQVVVTNPTHIACAL
                     RYERGKMRAPVLVAKGQRLIAEKIKKIAQENGVPIVENKKVAWELYKSCEIGQEIPSF
                     LYRAVAEILAFVYRLKATYGGRRNE"
     gene            complement(17713..18480)
                     /gene="fliR"
                     /locus_tag="PK016_02475"
     CDS             complement(17713..18480)
                     /gene="fliR"
                     /locus_tag="PK016_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HXL01972.1"
                     /translation="MDFTFFLSHFLTLLLVWMRLLGLFLLAPVFNSPLIPVLAKVGLS
                     FLLTVTLLPSLTSQDSFTSLDGYYLLALVSEILVGLVLGLLSGLVFSCIQIAGEVIDF
                     QMGFSYANVVDPLSNLGFSVMGQFYFLLTIIYYLGIGGYRLTLQGLVRSFSLIPLGSF
                     KLGLNATTSFVGLVGEVMVIALQLGAPVIATLFLSNLTLAIISRAVPQMNVFIVGLPL
                     NIGLGLFMSLMILPYFLPYVDEVVSLFLRSFFQIFAP"
     gene            complement(18484..18753)
                     /gene="fliQ"
                     /locus_tag="PK016_02480"
     CDS             complement(18484..18753)
                     /gene="fliQ"
                     /locus_tag="PK016_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HXL01973.1"
                     /translation="MSEELLLEIGRETLLIVLKLALPILGVTLIVGILVSIFQAVTQI
                     HELTLTFVPKILATILVVAFLGSWMLRSLLDFTNNLFWNLPLFLR"
     gene            complement(18769..19530)
                     /gene="fliP"
                     /locus_tag="PK016_02485"
     CDS             complement(18769..19530)
                     /gene="fliP"
                     /locus_tag="PK016_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307429.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HXL01974.1"
                     /translation="MAKKAIVLVLASLILLFLSSSAFSQEIPLVFPKITVEAGEVNEP
                     QELVLSLQILLLLTILSLAPAILIMVTSFTRIIVVLGFVRNALGSPQIPPTMVLIGLS
                     LFLTFFIMAPTFSEIQENALSPYLAGNITQKEALDRGVDSLRNFMFRQTQEKDLALLV
                     SFTDLPRPKTRSDIPTHVLIPAFILSELKAAFTLGFLVYIPFLVIDMVVASILMSMGM
                     MMLPPVMISLPFKLLLFVLVDGWDLIARGLTLSFR"
     gene            complement(19523..19981)
                     /locus_tag="PK016_02490"
     CDS             complement(19523..19981)
                     /locus_tag="PK016_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HXL01975.1"
                     /translation="MRRFCKILFFLGFIVITRGIVFAQEEELNLPEIQPPVSSSDGGS
                     ALRFIGAFLVVVAILWGFFYLMKRLTGQNLRLSSSRYMNLVDFLNIKPNLSLYLVEVG
                     DRVIVIAQSGNTIQEITELSKDELEENASFDGFSGYLDSWFGRKKDKRDG"
     gene            complement(19983..20972)
                     /gene="fliY"
                     /locus_tag="PK016_02495"
     CDS             complement(19983..20972)
                     /gene="fliY"
                     /locus_tag="PK016_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch phosphatase FliY"
                     /protein_id="HXL01976.1"
                     /translation="MPEDTLTLEEKDVIGELGNISMGAAATALSTILGRKVEITVPRV
                     EVINKEEVTSLFPEEHIAVKVQYREGLQGSNLLLVKEEDARTLISLMMGGAEAEEALG
                     EMEISALGEAMNQMMGSAATAMSEFLKRKISISPPEVIGQEVQSTEEEWLEEQEEEHL
                     VEVFFHLVVDGAIDSNMIQLLPFSFSRAIVSSLTVKQKEAVLPDEEASMTETPPRKEG
                     QEVRAQSVEFAEFKKKEEAPSRINMQLIMDVNLPLVVELGRTQLSIREILDLGPGSIV
                     ELDKLAGEPADLYINDVLFARGEVVVIEENFGVRITEIVSPEERIKTLKEARA"
     gene            complement(20973..21953)
                     /gene="fliM"
                     /locus_tag="PK016_02500"
     CDS             complement(20973..21953)
                     /gene="fliM"
                     /locus_tag="PK016_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HXL01977.1"
                     /translation="MSDILSQEEIDALLKALNSGNLEAEAIREERKEGGRFKVYDFRR
                     PEKFSKDQIRTFHMIFSALARLASTSLAAYLRSRCQVELVSVDQLTYDEFIRSVPNPT
                     FVCIFSLPPLEGRCVLQMNLDMVFPMLDRLLGGSGSTVFHPRPLTEIENRIMLEVTER
                     IMSALKEAWANIQQVEPQIEALESSLEFVQVVSGNDMIILFTFSVEVGEREGMMTICI
                     PYLVVEPITQRLSASLWFASRKSNDPQIQEKVKKKINQVRLPVVVELGRTNISLRELV
                     SLEIGDVVVLDKRAGEPLNILVGKKTKMKGMPGKSGRRLAIKIMELEEEE"
     gene            complement(21963..22442)
                     /locus_tag="PK016_02505"
     CDS             complement(21963..22442)
                     /locus_tag="PK016_02505"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HXL01978.1"
                     /translation="MPEKTENSEEKKEKGFSFQMIILVVTLVLLAFMVFVYLQQSGIW
                     SKLFASSPQEVNSAPSFIYIFEQNFLVNLSDKDVLRYLKITLGVEVPNQRVVDEIKNK
                     IVEIRDAIITILSAQSSEDLSTTEGKEKLKTLIADSINKFLTSGKVTAIYFVDFVMQ"
     gene            complement(22457..23176)
                     /locus_tag="PK016_02510"
     CDS             complement(22457..23176)
                     /locus_tag="PK016_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXL01979.1"
                     /translation="MPRKRAEEETPPGAPLWSLTYGDFMSLLLCFFIMLFALSTIDVA
                     RFKEVISSIQGALGVLEGGPRVLNPSDIPVPKPPTQISPSGLVELKLAGLMKEIERQL
                     IKEGHLSPDNVSFKIDERGLVITFLDTVFFDVGKADLKPGVFPILDALADALEEVENS
                     VRIEGHTCDLPIHTSQFPSNWELSAARAIAVLRYLVEQKGLPPERLIAVGYGEYRPLV
                     PNLDEEHRQKNRRVEIVILRD"
     gene            complement(23187..24008)
                     /locus_tag="PK016_02515"
     CDS             complement(23187..24008)
                     /locus_tag="PK016_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HXL01980.1"
                     /translation="MDKATIIGLVVGVVLLFWGMTNAAANNMRIYYDFASILIVGGGT
                     LAATLISFHLEQVMKAFQLFMIAMRSRLPQPSEIIITLVGLAEKARKEGLLALEEETE
                     RIEDPFFKRGIQLVVDGTDPQLLKNILETELIFLEERHKKGKAIFETMGAFAPSFGLI
                     GTLIGLVAMLVSLDDPKKVGPGMAVALLTTLYGAILANLICLPIANKLKLRSSEEVLL
                     KEVIIEGILSIQAGDNPRIVEEKLKSFFAPSIREELEREREEVGKVIPLKRESEV"
     gene            complement(24011..24238)
                     /locus_tag="PK016_02520"
     CDS             complement(24011..24238)
                     /locus_tag="PK016_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HXL01981.1"
                     /translation="MITLTRFNGSSFVLNMDLIETIEATPDTIITLVTGRKYVVKESV
                     DEIIGKILDFRRQSGVSKILIQNLPENELEE"
     gene            complement(24289..25506)
                     /locus_tag="PK016_02525"
     CDS             complement(24289..25506)
                     /locus_tag="PK016_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HXL01982.1"
                     /translation="MMRSLFSGVSGLKNHQTRMDVIGNNIANVNTVAFKSGRVNFEDI
                     LSQTVEGARSPQTGGMGGVNPKQVGLGMGISSIDTLFTPGSLQSTDNPTDFAVQGEGF
                     FIVSDGSQVYYTRDGAFKTSADGALVNSNGFRLQGWMADADGNIDSTQALQNITIPVG
                     AQMKPKATTEIAFEGNLDANAADSSQWITSVQVYDSLGNPHNLTITFTKTATANEWGW
                     AADLDGISGDSGAIEFNPDGTVFSGGTGMASFTSIPGADSIGLSIDFSQLTQFGGNPT
                     AYISFQDGYEAGSLDMVSTDSGGVLTGIFTNGQSKALAQIALATFSNPGGLLKEGGNL
                     YQISNNSGLPNIGTASSGGRGSIAVGALEMSNVDLAQEFTNMIVTQRGFQANSRVITT
                     SDEMLQDLINIKR"
     gene            complement(25551..25916)
                     /locus_tag="PK016_02530"
     CDS             complement(25551..25916)
                     /locus_tag="PK016_02530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02530 family flagellar biosynthesis protein"
                     /protein_id="HXL01983.1"
                     /translation="MVQKIENFREFIPGIQPSTPLREKAKTTANFEQVLQEEELSFSL
                     HAQRRIKEREIPLSPDTVERLQEAVQKVKEKGGRNSLVLVGEVAFVVNVPERVVVTCM
                     DSNSRKDNIFTNIDSAIIA"
BASE COUNT         7819 a         6015 c         4557 g         7565 t
ORIGIN      
        1 ggtaaggttc ctcgcgttgc atcgaattaa accacatgct ccaccgcttg tgcggacccc
       61 cgtcaattcc tttgagtttc aaccttgcgg ccgtactccc caggcggggt acttaatgcg
      121 ttaactgcgg cacggagggg gtcgaacccc ccacacctag tacccatcgt ttacggccag
      181 gactacccgg gtatctaatc cggttcgctc ccctggcttt cgctcctcag tgtcagggac
      241 agtccagaga gccgtcttca ccactggtgt tcctcccgat ctctacgcat ttcaccgcta
      301 caccgggaat tccactctcc tctcctgccc tctagcttct cggtttccca cgaccctccc
      361 cagttgagcc agggaatttc acgtgagacc taagaaacca cctacgagct ctttacgccc
      421 agtaaatccg gacaacgctc gctccttacg tattaccgcg gctgctggca cgtaattagc
      481 cggagctttc tccggggata ccgtcaggga gagaagctat tcactcctct cctgttcttc
      541 tccccgaaca ggagtttacg acccgaaggc cttcttcctc cacgcggcgt cgctgggtca
      601 ggctttcgcc cattgcccaa gattcctcac tgctgcctcc cgtaggagtc tgggccgtgt
      661 ctcagtccca gtgtggctga ccatcctctc agaccagcta cccgtcatag ccttggtagg
      721 ccattaccct accaactagc tgatgggacg cgagcctctc tcggggtggc agcttgcgcc
      781 acctttcctc cttcctctta tgagaaaaga gaatatgggg tattagccac cttttcagat
      841 ggttatcccc ctcccccagg ggagttactc acgtgttact cacccgttcg ccactcatct
      901 gtaccgaagc acagatgcgt tcgacttgca tgtgttaggc acgccgccag cgttagtcct
      961 gagccaggat caaaccctcc atgaaaactt ttaatcaaag ctcattgaga gcttgatctt
     1021 gcaaaatttg tatatacact cttcaggaaa taggcggaat attcatcttt cagggtgctc
     1081 cctcttccgg agaagaggca aggagtaatt taccacagga gcaagggatt tgtcaatggg
     1141 gagagagaaa taagataact atttattctg gcattctgac cagtacttaa aacaagaaag
     1201 gaggctcgaa aacgaactcc gctagatgag gatgacaatt ctcaaagaga aaaccgggaa
     1261 atttttattc ttttcttcca gaaacaccaa tataggtatc taaagcggtt gaatctctca
     1321 aaaaccagga aatatttctc accttctaca aggcttcagc ttacattcgt aacatcttcg
     1381 aactactata gtatcccgta aaactggtta aaaactttag gttgttacac acagagaaag
     1441 actttgtctt tctctgtggt tcttgtcttt tgtgttttgt gtttgtttct ttgttctttg
     1501 tcttttgggg ggttataatc tcatcattac ccataagttt tttctggaca ccatagaagg
     1561 ttttcaaaac agataaatgg atagaaagga ataactacta actacctggg agattaaact
     1621 atatcctcta aaccctgtaa acccaatcat ataagttcaa gagagatatc atagttaatt
     1681 tatgtactca ccgaggagat ttctaatctt cttgaagagg gacaatatag caaagcaaaa
     1741 agacggatca atcttcttca tcccgtggat gttgctgaga ttatggcttc tctcgatttc
     1801 cgaaaacagg cacttctctt ccgtcttctc aataaaaatc aggctattac agtgtttgaa
     1861 caactcgatt ttgagcaaca gaaaatcttc tccgccattt cactgatgag ggagtagctc
     1921 gaattttaaa ccagatgtct cccgacgacc gaacggaact ttttgacgaa ctccctacta
     1981 aagtggtcaa gaaattcttg gatcttctgg aacctgaaga gcgcagcatt gctgccagga
     2041 tgctggggta tccagaaaat agcgctggca ggattatgag cacccatatc attgacctta
     2101 aagaatttta tacggtagaa caagcactaa atcccggcgc ttaagccccc cagaagaact
     2161 tgcttgcact gcttatatta ttgatgcaga gcatcacctt ctaggaatag tgactctccg
     2221 aaaccttgtt cttgcttcac tagaaaagcg agtaggggaa attatgagcc gggattttgt
     2281 ttttgtttcc actgatgacg accaggaaaa ggtagctcga atcgttcaaa aatatgacct
     2341 tcttgctata ccggttgttg accgggaagg tcgactggta ggagcagtaa ccatcgatga
     2401 tgtcattgat attattgaag cagaagctac ggaagattta caccggctgg ctgctatcgt
     2461 aattaccgaa gatgagcatc ttcgcgctcc cctgggacag aaaattaaaa gccgcttctt
     2521 ttggctcgct attcttctca ttatgggaac tttcaccagt tttgtaatgc aaagctttga
     2581 gggcattctt cgagctgccg tagcactctc cttttttatt cccatgctca ccgatactgg
     2641 gggaaatttg gggagccagt ctaccactgt ggtggtaaga gagttagctc tgggaaaact
     2701 ggaaggtcat tttctctgga aaatgattct ctctgaagct attatcggtg taactttagg
     2761 ggttcttcta ggaattgtta ctgttgcacg ggtcgtcttt ctcagagaat cactacttct
     2821 tggccttgta ctagcgcttt cagtatagat aattgttttt gtttccaacc ttgtcggtgt
     2881 gtttttaccg gtgcttttca agaaaatgaa actcgacccc gctattgccg ctacaccagt
     2941 aattaccacg atagcggata ttataggggt tctcatatat ttagagatag cacagatttt
     3001 attaccactg taagaagtta gtaatttcca aattattgtc atcacatagg taaagaaaaa
     3061 cgataaacct tttgtggtaa aataacaaag gtaagaaggt ttattcttac agaaaccgag
     3121 aaaaaaaggt gaaagacttg aaaataggaa tcggttccga ccatgctggg tttgcgttca
     3181 aagaagcaat aaaggagaat ttatcaaaag aaggacatga agtgattgat gcaggtgctc
     3241 aggatgagca gccggtagca actgctcctt atgctcaagc aatagctgaa ggtttattaa
     3301 atggtgaatt tgaacgaggt attctcattt gtggaacagg tggaggaatg tcagttacag
     3361 ctaatcgcta tcctggtatc cgggcagtac tctgttttaa tccctttacc gcccggatgg
     3421 ctcgcagcca taataatgcc aatattttag ttctggggtc tcgtactata actctacctg
     3481 aggctctcga gttagtagat atttttcttt ccacttcttt tgctggagga aagtatgctg
     3541 aaaggcttaa atatctagaa gagatcgaaa agaaagtagc aaagatttta caggagcgat
     3601 tcgcttgaaa aaggaaacgt cttcatattc ttttcgggta gtttggagta tttattaatt
     3661 ttggaagata ttaataataa agaataaaat ggggaagaag aagacgcctg ggtgagaatt
     3721 gatgtgttgg agagtaaata aggaaggtga aaaatattgc tgcttaagat aatggaccgc
     3781 tatttaatta aagatagtac tagtggcatg ttcacctggg taggagcagt aactgttata
     3841 atgcttgtac agactctttt tgagctagct gacttctttg ttaacaaaaa agtccccctc
     3901 ctcatcgttt tggaaattct tctcctttac attcctgctc atatggtgat gaccttcccc
     3961 atttctggtt tattagcgtc agaactcaac ttaggacgtc tttcccggga tagtgagtta
     4021 atagctttag aagccagcgg agtgagctta agaagagtta tactgcctta tatcattttc
     4081 tctattttgg tgagcgtggg gtcttttact ctcaacgatt tagtagttcc tgagaccaat
     4141 caccgagccc aggttttgat ccgggaatat gtctataaag aaggtcctcc ccagattgaa
     4201 aaaaatgtgt ttttccgaga ttctcagaac cgttattttt atgttaacga actggataat
     4261 aaaacctggg agatgaaaga tgtcattgtt tatgaaatag gtaaaaatcg ttccttccct
     4321 gatgttattt tagctaaaag tgctttatgg ttagaggatc gctggttgtt gaaagaagga
     4381 gtagtacatc gctacaatga gaacggttta cttattcagg aaattgaatt ttctgagatg
     4441 gttatagata tgaaagaaga gctaaaaagt ttttttaccg aacagagaag cccagaagag
     4501 atggcttctc gagaattaaa aaagcagatt gatattctga aagaagcagg agccaaaacc
     4561 gaaaccttcg aagtagctta tcatctcaag tattctatcc ccttttccgc tctggttttt
     4621 attttgatgg gaataccttt aggtattcaa cgtactaaag acacgagaac cataggagtt
     4681 attgttactg ttatattagc ttttgcttat tatatgttgc tttccatttt tcgctctctg
     4741 ggaagaggag ggataatgga acccataata gcagcctgga tgcccaatat tatttttggc
     4801 ctgcctggtt tcatactcta tttgacggtg gatagaaagt gaggtataaa atgaaaaaaa
     4861 tcctttttta tcttataata tcagtgctgg tcatctcctg gggtggagga tggttaagag
     4921 cggaagaaaa tctttttgac caagccatgg aagctcgagg aaaaggggaa gatgaagaag
     4981 cgctgaaaat tttcgagcgg tgtgtagaag aagggataga ggtgcatcaa gcttactatg
     5041 aaatggggaa gatacttctg gaccagaaaa aatatcggcg agctattatt gtttccgaaa
     5101 aagctataga agcttacaaa gaacatttga aaaatcaccc agaagaccac aatgcctggt
     5161 gggaattagg cgatatttat ggtactagaa gtaattataa ttactctgat gagtggaaag
     5221 aagctcaaga ggctttagaa aaagcagtag aactttctcc ttctaattcc ttttatcttc
     5281 tctacttagg ttggatttat tatcaagagg gggacaatga aaaagctgaa agtatcttta
     5341 gaaagatact ggataaaaat cctgaagatt ttcaggctcg ccgtttttta attgatgcat
     5401 taattgagga aggaagaaaa aaagaagcta aagaagaatt ggcttttatt ttagaaaaat
     5461 gtcctaagga ggatgagtta tatagttggg cagaaaaaaa atgggaagaa ttagaggggg
     5521 aaaatcaatg aagactaaag ttatcttctt aataagttta atcctggtag taggtgtttt
     5581 ttctggctgt ttttcggtga cacaaaaggg ttctttagaa ggagaggtat ggggagaaag
     5641 cgggcccctt gcaggagcgg tggtggaggg gggaggaagt acatctatca ctaacgaaga
     5701 aggaaaattc cttatagaag gattaccatc cggagaaact tatcttttct tttcctgtct
     5761 cgggtacaca ggtaaagcga taaaagtaga agtgaaagct ggagaaacgg gaaagattaa
     5821 tcaagaaggt agaatatatc tggttcctca ggatgaagaa agccttaagg attatatttt
     5881 tgagctctat agtctgggtt tttatgagcg agctatctct caggtagact tgtttcttac
     5941 tgattatccg gaaggaaatt cattacccca ggtccttttt atcaagggag cttctttata
     6001 tcacttgaaa aattttcaag atgctatttc tcttttgtct catatcaggg acaattatcc
     6061 ggataacgaa ttttctgatg atgcgcagta tctgctggct aaaagtttcg gcgaagggtt
     6121 aaaaaactac gaaaatgcta ttgtagaata ccagcgattg atagatagtt atccagatag
     6181 tgaattttta ggcatagctt attatgaaat gggagattgt tattacatct taggctctta
     6241 ctctgaagcc tacctcagtt accagaaagc tcaaacttat ggtggggaag tagagaaggg
     6301 ggctctctat tcttccgctc attgtcttta taagctggaa ctttatcccc aggcggcgct
     6361 tcttttttct gaatatgtag aacgttatcc tcagaccgat atatcggatg atgctcaata
     6421 ttttgccggt gctgcctggt ataaagccga ggaatacagt caagctttag tcgcttttca
     6481 acattgcgtt aataactatc cccagggaac ctggtacaat gggattctca tcgctccagc
     6541 agcccttttg aaccaaggtc tttgtttaga aaagctgggt agataccgag aggcttataa
     6601 catttatctc cagattatcc gggagtatcc cggggcaaag tgggctgacg gtagctcttt
     6661 aattaaaaat gttcaatttc gtattgaatg gcttgaaaat aatgtcttgt aaaaccaacc
     6721 aacatgcacg aagaaaaaag cgatcgggaa cttattactg aggtattaaa tggtaatgag
     6781 gacctgtttt attttttagt acgccgttat gagaagcaaa ttttaaatta tatctatcgt
     6841 ttagtaaaac agagagagga agcagaggac ttagctcagg aaactttcac caaagctttt
     6901 tttgcccttc gccaatatga tcatacttac gagttttcta cctggctttt tcgaattgcc
     6961 cttaatgttt gtcgagatta ttttcgaaga agaaagattg cctttttctc tctaaatact
     7021 ccagtagggg aagaagaaga aacagagtgg ggagaattaa tagagcagaa ttcttttcca
     7081 gacccagatg gagagctgga taatcgggaa ctacgaagag aattagaaaa agccattgac
     7141 catcttcctc ttaagttcaa agaagtaata gttttgcgcc atttagaagg tttatcttat
     7201 agtgatattt ctgaaattac taatttgccg gcgggcacag taaaaactta tcttcaccgt
     7261 gctcgtaaaa agctccagaa agaacttaaa aaatatctgg attaaacaaa aaatgaaccg
     7321 gtaggttatg atatcagcta actcgagtac gcaacacgaa aactttaagt ttatctattt
     7381 ctctttctgc tttgataatt tccagagctc ctcgtcactt aatccaaagt aatgtccgat
     7441 ttcgtgccat aaaactttct ctaattcttc tggccaattc ttaccggcta ttctttccag
     7501 attttcccta tagattacta tccggtcagg catcaccagg ttgtaattgc cacttctccg
     7561 aggaagggga ataccctgat aaagtcccag taaaaaacga gagccaaacc gtccttgaat
     7621 ctcgctatcc ggataatcct caactacaaa ttctacgtta cttatattat ttttaaatag
     7681 aggaggaagt ttttccagaa gttcctctat caataaagaa agttcctttt tatctattgt
     7741 cacaataatt ttttctcctt cctctgtcag aacattaaaa ttatacctca taaataatag
     7801 ttgcggtttt aagcacaaaa tccacagcaa tgttcctcta ttcagcactc tatataccag
     7861 ctccactgga aagtaaaact cctccatcat cttttaatac tggaaaagaa acggaaaata
     7921 acccttacaa atgcagcccg ggaagaaatt gccacatcag gcaaaaagag gaatctacaa
     7981 agatagagaa gaagagtcag gatgaattgt tagaattata aaataactca tcttctaacc
     8041 cctgaagagg aaatattaca gtattattta tcacaactca accagcgata ttcaacacca
     8101 caacgaattg aagaaagtct caagaataaa ataaccagtt ctcgattgtt acactcgaac
     8161 atcaacaata ttccccttat ctccttttac atccagttgt ttcttctcct ttttctcctt
     8221 tttctccttc ttctccttct tctcggtatc tatcatgtct ctttcttctt tcttcttctc
     8281 ttcctttgga taacggtcag tttccggaga aggaattact tctctctcca ccttttgaga
     8341 ctcatcttgt gcttcttgag ctaaagcata agatgataaa gtagaagcat tatcttccac
     8401 tttctgaata cgggctacct ctggggaacg aatcactaca ttctgcatac ttactgggtc
     8461 taccacaaaa accaccctcc tcagcttcga ggtttttcaa aactccccaa gactacttcc
     8521 tggtctttct tgtaaaagga agcgtaagga atttcatccc tcacgggata ggtaagatta
     8581 cccaccgtga ttcttacctg aggataaact ttttcttcca ccaagacttt cccttctcct
     8641 ccagaaatta tttcttccaa ttcttccaat cttgcctcgc aaaattcttt tttttctctg
     8701 gctaatcgac aagttttttc taatttttct aaaactttta tcataccagg atcaagacct
     8761 ttccccacct tttgtctctc actaatagtt ctaaaaactt tttccccttc ttcaatctgc
     8821 tttttcactc tttctaactc ccccataaga aaattatact cttcccggag agtagggtct
     8881 attcctacca ttacttctgt gatagtgccc atagatgatc ctaaggtttt tacccatacc
     8941 aaattccctg cttttatctt tcctcccact gccaaacccc gtcctcctac taccactttt
     9001 ctacctgccg aaatctgact atgaaatatc gcttctccta ctataacatc ttgacccgct
     9061 tctactgtag cattttctat tacttttgct tttacatctc ctcttgctct caccaagcct
     9121 ttttctcgcc caaatatccc cccccttatc ataatgtttc cctgagactg cacaattgct
     9181 gcttccacat taccccaaac ctctatatct ccttccgcct ccaccttaaa accactacgt
     9241 atcgtccctc gaatcactat agaaccaata aaagagatat tgcccgttga agtattaaca
     9301 tccccatcta cttcaaaaag gggcaaaact gatatagttt tgcctatcaa aaccggtctt
     9361 cctgctacct tagcaacagc cttggtaccc tcttctacta actctacgtt cttccccact
     9421 cttattttgg ccggtcttcc aaggagagcg ggaatttctt ttccgaaaat gtcctgaccc
     9481 ggtacacctt ctcccggagg aataagctcc gcaagtaagt ctccttcctt cacttctggt
     9541 attttcacaa aatcaaataa gtccaccctt ccactctcat cttcttctaa ctttttttcc
     9601 aattcttggg taaataaaat ttttacctcc ccatccttcc ctctttgtgg ccttttccct
     9661 tcagcaacaa gatgacggcc ttcttcttgt aaaagttttt tccccctctc caatacctct
     9721 tcttttattc ccacagttat tcctctacta ttcaaaaagt cttttaactc ctcataagag
     9781 gaaagttctt cctcgctaag aggttttttg accaccaatt ccgatcgtaa accatcacca
     9841 gaaataaaaa gctgaatccg attatcctct tccaaaagct taagagactt gagcaactat
     9901 acctccctct acttccaatc ggaaagtttt gtctttagtt ttagaagagc acgagaaata
     9961 atctgagaaa ctcgtgactc tttaacttcc aaaatagcac caatctcttt gagagtcaaa
    10021 ccttcaaaat aatagagagt aatgctcaat ctttccctct ctggcaattc ttcaatagct
    10081 tttcctaaat agtctaacag ctcttcattc tccagaagag cttcaggatt aaacattaag
    10141 cgctcttctt ttactccatc aagaagtttt ttcccctcct ctttttcttc ttctaaagaa
    10201 aagaaaaagg cagatttacc ccgctctatt atccaggtta cctccccaac agaaatatct
    10261 aactcctttg ctatttctga taatgttggc tctcgccctt ttcttttctc tagctcctgg
    10321 taagtagcca taacttcttt ccaaccccga cgctcccggc gagttaaggg gtctaaactt
    10381 cgcaaataat ccagcaccgc tcctctaatt cgagacaggg caaaagtctc aaatcgcaaa
    10441 cctttatccg gtcggtacct atctaaagcc tggagtaaac caatcactcc ataactttcc
    10501 aaatcttccc tgtctacata agaaggaaga atataaagta accttcccag agctatttta
    10561 accagataga gataatgttc tgccagtttt tctctttctg cagaatccct agtcttaaga
    10621 tagctctccc agactttgtc tatattttca atctccggag ttagattcat cttttacttc
    10681 cgtttcctta tttccaccag aagaaataat ccataaaaaa taataaaaca taattagata
    10741 agccacagaa aaaagtaaaa gccccctcca taccaaagtt aaaaaactta aattacgcca
    10801 gaagccccaa ataaaatata ataccaacaa aatccaggat aaaaaaagag caagaaggcg
    10861 cgccaggttt ttggcctgtc tgacttccat ttctaaacta ccacaggttt ctgtccaata
    10921 gtttttattt ccagtcttcc atcttcaagg taaaacataa tactcctccc ccgatttcct
    10981 cctgtatcat ctgctcgtaa agggatattt aatttttgaa gctctgcctt aaccacctca
    11041 acatttcttt taccaatgtc aaaaagagaa tccgttcctg aaaacatctt cgatcctcct
    11101 gcaatcttgg caaaaacatt ttttggtgaa gcacctccgt ttttaagctt cccccacagt
    11161 gcaggaactg cagcatcagc aaatttaaaa ggattactct gccctggaat actttcagga
    11221 agaagaatat gaaccatacc gccaattttc ttaacagggt cataaagaca caaacctaca
    11281 caagaaccaa gtcccagtat acataaaaca tcctcgggat tactactcac ccaatactct
    11341 gccattccta ttaccactcg attgtacatt ttagattact cctatagacc gcagaattat
    11401 ttccagagac ctcgtatccg gtataagcat aaaataacct tttattctgt tttcttcttc
    11461 cacaaattcc gtttctataa gcagggcata atcgcctttc ttagaaatct caatcaatat
    11521 cacatctaca atagcaccag ccatatcaat agccagagca ggaaccgaat gattcagtcg
    11581 cagaccggta aaatcagcca aagcagataa ataagaagaa gaaaggatat ttcctatctc
    11641 ctccattgct gaccgggcta tctcatccat ctctaatacc tgttcccgag aaggagtaag
    11701 gtctcctaaa agaatatgca tgattttcaa ggcatcttca atagtcataa caaagagaat
    11761 gtggccagtt gcagctccag acatggttaa ataaactcct aaaacctctt tttccggccc
    11821 tcccagccaa tcaggaacgt ctttaagagg taaaatctta accaggggta cttccatctt
    11881 tacccttttt cctaccactt tagataaagc agtagcagcg ttacctgctc caatgttacc
    11941 tacttctcga agagcgtcca gctgtatttc attcaatttt tcaaaagaaa tttccatact
    12001 cactcaccca cctttctgat aaaaaaaggg caaaacataa cgtaacccta aagaagaagg
    12061 atcggtaatt cgttcggtac ttcccaccca caaatatccc cctttttgca aagcgtcact
    12121 cattttttta aacgcaattt ttttagcctc atcttcaaag taaataatca cattacgaca
    12181 taccacaaga gcaaaagttt cttttacctc atcctgcaaa agatcatgtt tttcaaacgt
    12241 aattcttttt ttcagttgat gtacaacagc atattttcct tctccttgaa actgaaaata
    12301 tttctctaaa taaacagagg gaacattgct tataaaatct tcagaataca cgccttcctt
    12361 agctttctct aaagcatctt cgtctatatc agtagcccag atacgatagg gtttacgatg
    12421 aggcatctcc tctaatacca tagctaaaga atagggttca cttcccacgg agcaccccgc
    12481 actccatatt tttaaaatta aacctgcctc tttcataagt tttggtaata ctttattttt
    12541 taagtcttca tagcgctcag gattacggaa aaactctgaa acattaatgg caaaacgatt
    12601 tttaaaatct tctaaaacct gaggattttt atctaaaagt tcaacgtatt cctccacact
    12661 ctgagcaccg gctctactta taataaaagg aagacgcctt tgcagctgat tttccttata
    12721 gtaagcaagg tcaattcctg tcttttctaa cactttatca cgcaaatact ggatatcttc
    12781 tctctttaac attttagctg ccatctcccc gggaatccca ttctagaatt gctgagggaa
    12841 tatcttgtaa aggtaagact ctgtcagata gcttctcctc tattactgct ctgggcatac
    12901 caaatatgag agaagttgac tcatcctgag caataactgt ccctccattt tttttgagag
    12961 ctcggcaacc ttctaaggcg tccttcccca ttccagtaag aactacagct aaaatattct
    13021 ctcctccaaa gacttttacc gccgaaagaa gagtaatatc agcacagggt tttaaacctt
    13081 tcaccggagg atcatcacta aggcgaactt ctttactctc ttctattata agatgctttc
    13141 cccctggagc gaggagagca caattcgtaa ctaacttatc ttcgttgctc gcttctttaa
    13201 tgggaaaagg agcagagtta ttcagccttt ccgccaggct cttggtaaaa ccactgggca
    13261 tatgctgaac tatcaacatt ccactttttt ttaaagggga aagattatta acaagagtgt
    13321 gtaaaacctt aggaccaccg gtagaagcag ctataaacac caaacgagga cattcccttc
    13381 cttccttttc ccgaacagag attttcttcc tttccctgcc ttgagtgaca aatttcccta
    13441 cctgagaaag aggaatagtg cttgctgatt taaccttggc aattatttct tcctttttct
    13501 tttcaatatc taaggatata ctccctgaag gtttaggaat aaagtccagt gcccccttat
    13561 ctaaagcttg aatagtgatt tcacttccct cctgagtcaa agcactgagc attaccaccc
    13621 gacggggacg ctcctcaaaa attttttcga gcagtcctaa accatccaaa cggggcattt
    13681 ctacgtccag aagaataaca tcagggtcta aacttctcag tttatcccag gcatccaatc
    13741 catcccgggc ggtagctatt acctcaattt ccgggtcaga attcaataaa tcactaacta
    13801 tcttgcgcat aaaactcgag tcatctacta tcataacttt gattttcttc atcaaattaa
    13861 aagtcctttc tttcttattt ctatctgttg cttaaaaata taatggatga tttcttcctt
    13921 atcctggtca gttatttcca caaactggaa agcataacag gaggggtcat cttttcttac
    13981 tactttagct ctaccaataa tacttttatc ctcaagagaa agagaaaata ctacttcgtc
    14041 tccaatcccc aggaagtttc tctccccgct aaacgaggct tgcattcctc ccccactcag
    14101 gtttctggta taaccaagaa aagtcctgtc atcttttcta attaagagcg ggatcgcaac
    14161 atctattctt acataatttc ttctctgctc cctttttacc tcctgaggca aactcaaaat
    14221 gaggaatgcc atattctctt tcctcagccg atttttgact tctccctcaa aatgaaagat
    14281 ctcattttct cctataacat aaacaaataa tttttctccc actcctatcg gaactaaaac
    14341 acctttttct tgaggagcag ccacccataa ttcttcttct ccaactaact caatacggct
    14401 aggataatag aaaactactt cttctccttt ttcatttttc ctattcatcc ctaaacttat
    14461 tctccgatta ggtcgaaaaa cctgagaaat tttcatgttt cctgaacttc acatccttat
    14521 cattccctct actttaacct ccacatcttt gggaatttcc tcaaaagcta gtactaccac
    14581 ctgaggtaaa taaccactaa ccaggtcacg aagacctctt ctaattttac cagttgtggc
    14641 cagaattggg aaatgcccta aaccaactat tcgttctaca ctctcactca aacggtctaa
    14701 aaaagaacgg aagaaatcag gctctaaagt ttcctctccc tgtaaagccc gttgcaacaa
    14761 attctccagg ttagtatcta aagtaaggat atgaagcttt ccatcttctt ccagatactg
    14821 ttttactatg cttctaccca gctttctcct caccatctca gttaaacctt ctatattttt
    14881 tgcctgagga gcataatcag ctaaggtctc aagaataaga gaaagatctc gaataggtat
    14941 cccttcatcc agaagattct gtaaaacttt ttgtacttct cctattgaca tagcctgagg
    15001 aatcaattcg tccactactg cctgatttac cttttttacc tgatcaatta gcatttgcac
    15061 atcctggcgg gttaatagag agggagcatg agctttaatt acttctgaaa ggtgagtgat
    15121 gagaactgat tctgaatcta ctaaagtaaa tccagcaatt tctgcttcct ctttattaac
    15181 ctcattcacc cagtaggctg gaagattaaa agccggttct ttagtgggga ttccctgaat
    15241 agtggccgct tctcctcggg gactaatggc cagataataa tgaggcatta tatctcctct
    15301 tcctacttct actccccgaa tttttatcac atactcatta gggcgaaggc gtaaatcatc
    15361 tcttacccgg ataggaggaa ctacaaaccc cttttcccga gccatattaa ttctcatctg
    15421 agttattcgt tctaaaagtg tacccccggc tgaagcatcc accagaggca ccaatccata
    15481 acctatttcc agctctatag ggtctacttc tataagcttc aatatttctt ccgggctacg
    15541 aggtagttct tctttcttct ccgatagagt aacagcaggt tcttctcctt ttttttcttc
    15601 tttcccctgc aaataataaa aaacaaaagc cgccgaggct aaaaggaaaa aaggtaattt
    15661 gggtaaacca gggattaatc ccagaactaa aagtaaaata gcagaaagga gtatcacccg
    15721 gggaacttga attaattccc gggttacctc ctgacctaaa ttatcttctg aagcagcacg
    15781 ggtgacaatt aagccactgg cggtagaaat caaaagagca ggaatttgag ctactaatcc
    15841 atcccctacg gttaataaag catacgcgga gagagcatcc tgaatgctca atcctcgctg
    15901 taaactccct actataatgc ctcctaaaaa attcaccagg gtaataatca agccagcaat
    15961 ggcatctccc ctaacaaact tgctggctcc atccatagct ccatagaagt cagcctgtcg
    16021 ctctatttct cgccttctcc gacgtgcttc tacctcgtct atgagtccgg catttaagtc
    16081 agcatcaata ctcatctgtt ttcctggcat agcatccaga gtaaaacgag ctgctacctc
    16141 agctatacgg cctgctccat tagtaataac tacaaattga attatcacca gaattaagaa
    16201 aatgataaag cccactacat agtttcctcc caccacaaag ctaccaaagg tagcaatcaa
    16261 ttttcccgca tacccctgca gaaggattaa acgagtggtg gaaacattta aggccaggcg
    16321 aaatagagta gcaaaaagca acagtgaggg aaaaactgaa aattgtaaag ggttggtgat
    16381 ataggtggtg gtgagaagaa tcataaccga tagagtaatg ttgcaactta aaagaaaatc
    16441 tactactgtg ggaggtagag gtataaccat catcaagatg atgagaatga agagaaaagc
    16501 aaagaggaaa tcgctatttt tgagactctt ttctaaggtt ttagttagag cttcattcat
    16561 ttcttcttcc tccataagta gccttcaatc tataaacgaa agctaaaatt tctgctactg
    16621 cccggtataa gaaactgggg atttcctgcc ctatctcaca gcttttatag agctcccagg
    16681 ctactttttt attctccact attggaactc cattttcctg agctattttt ttaatctttt
    16741 ctgctatcaa tctctgcccc ttagctacca gtaccggcgc cctcatttta cctcgttcat
    16801 agcgaagagc acaggcaata tgagtggggt tagttaccac cacttgagct ttaggaattt
    16861 cttgcatcat cctccgccgg gctatttcac gctgacgccg tcgaatatga gactttatta
    16921 tcgggtctcc ttcagttctc cggtattctt cctttagttc ttctttggtc atctttatat
    16981 ttctctcgta ttcgaaacgt tgataaaaaa aatcaaaaaa acccactccc agaaacaagc
    17041 cccctagata aagccccaat ctttctgcta tctcttccac cgtttctatt ccagcaagaa
    17101 gctccatata aataaggtca gtgaggcgag gcagagcatc ctttactacc acataggcca
    17161 acactatccc cagaaaaact ttgatagata ttttcaaagc ttctactaaa agacgcaaag
    17221 aaaaaagtct ctctaacccc cgcactgggt taacataatc aaaattgggt ttaagctttt
    17281 ctcctgatat tacaaagcca gtttgcaaaa aacctaaaaa taaagctaaa ccagcggcta
    17341 ccactacaaa aggaagcact cctcgagcga aatatcctac tccaatctga gctaaatgga
    17401 aaacccactg agaatctacc tctcttgagt agtagtttcc cgcccatatt tcttctatca
    17461 aactcatcat ctccctgctc aaggagggaa aaataaaggt ggccagaaga aagagcacaa
    17521 taaagctaaa gccgctggta aaatctacac taataggaac ttttcctttc tggcgtagct
    17581 cttctcgacg acgaggcgta ggttcctcag tcttatcctg ctggggaggc atactttcac
    17641 ctctttttat acttcaataa ttacaattct ttaatttcag gataaaattc tttgccaaaa
    17701 tgacctctca acttaaggag caaatatctg aaaaaaactg cgcaaaaaaa ggcttaccac
    17761 ttcatcgaca taaggtaaaa agtaaggaag aatcattaaa ctcataaaca aacccagacc
    17821 gatattaagg ggcaacccta caataaaaac attcatttgg ggaaccgccc gggaaataat
    17881 agccagagtc aaattagaca aaaaaagagt ggcaattacc ggtgccccca actgcagagc
    17941 aataaccatt acttctccta ctaggcccac aaatgaggta gtagcgttta aaccaagttt
    18001 aaagctgccc agagggatga gggagaaaga acggactaaa ccctgcaaag taagacgata
    18061 tccccctata cccaagtaat agattatagt cagcaagaaa taaaattgtc ccattaccga
    18121 aaatcctaaa ttggaaagag ggtctaccac gttagcataa ctgaaaccca tttgaaaatc
    18181 tattacctcc ccagcaatct gaatacagga aaaaaccaat cctgacaata accccaatac
    18241 cagtcctact aaaatttcag aaaccagagc taaaaggtaa taaccatcca gtgaagtaaa
    18301 agaatcttgc gaggtaaggg agggaagcaa ggttactgtc aaaagaaaag aaagaccaac
    18361 cttagcgaga accggaataa gggggctatt aaacaccgga gcaagaagga aaagacctaa
    18421 aagcctcatc cagaccagca aaagagtaag aaaatgagaa aggaaaaaag taaaatccat
    18481 gggttagcgc aaaaaaagag gcaagttcca gaaaagatta ttggtaaaat ccagaagact
    18541 gcgcaacatc caagaaccta aaaatgccac tacgagaata gtagccagaa tcttaggaac
    18601 aaaagtcagg gtgagctcgt gaatttgagt tactgcctga aaaatactca cgagaattcc
    18661 tactataagg gtcacaccca gaataggcaa agctaatttc aaaacaataa gtaatgtctc
    18721 tctacctatc tctaacaata attcttcact caaatttatc tcctcccctc atcgaaaact
    18781 gagtgtcaac ccccgggcga tcagatccca gccatctact aaaacaaaaa ggagcagctt
    18841 aaaaggcaaa gaaatcatca ccggtggaag catcatcatt cccatagaca tcaaaatact
    18901 ggctactacc atatcgatga ccaaaaaagg aatataaact aaaaaaccca aagtaaaagc
    18961 agctttcaac tcgcttaaaa taaaagcggg aatgagaaca tgggtaggaa tatcacttct
    19021 ggtcttagga cgaggaaggt cagtaaagga aactaaaaga gccagatctt tttcctgagt
    19081 ctggcgaaac ataaagtttc gcaaactgtc caccccccga tccagagctt ctttttgagt
    19141 aatgttaccc gcaagataag gagacaaagc attttcctga atttcactga aagtaggagc
    19201 cataatgaag aaagttagaa acaaggacaa acctatcaac accatagtgg gaggaatttg
    19261 aggagaacct aaagcattac gcacaaaacc caaaactact ataatacggg tgaaagaagt
    19321 aaccatgatt aaaatagcag gagctaagct taagatagta agaagaagta atatctgtaa
    19381 agataaaacc aactcctgag gctcgtttac ttctccagct tctacagtga ttttcggaaa
    19441 aacaagagga atttcttgag aaaaagctga agaagaaaga aaaagaagaa ttaaagatgc
    19501 taaaactaaa acgattgctt ttttagccat ctctcttgtc ttttttcctc ccaaaccagc
    19561 tatccagata gccggaaaag ccatcaaaag atgcattttc ttctagttca tctttactta
    19621 attcggtaat ctcctgtatt gtattgccac tctgggcaat cacaatcacc cggtctccta
    19681 cttccactaa ataaagagat aaattaggtt tgatattcag aaagtctacc agattcatat
    19741 atcgagaaga agaaagacgg agattttgac cagtcaatct tttcatcaga taaaaaaacc
    19801 cccagagaat agctacaaca acaagaaagg ctcctataaa acgtaaagcg ctacctccat
    19861 cactagaaga aacgggaggc tgaatctctg gtaaattcaa ttcttcttcc tgagcaaaaa
    19921 ctattcctct tgttatcact ataaaaccga gaaaaaacag aattttacaa aatcttctca
    19981 tcctaagctc gagcttcctt caaagtttta atcctttcct ccgggctcac aatttcagtt
    20041 attctaactc caaaattttc ttctatcacc actacctctc cccgagcaaa taatacatca
    20101 ttaatgtaaa ggtcagctgg ttctccagcc agcttatcta actctactat cgaacccgga
    20161 cctaaatcta aaatctcccg aatagaaagc tgagtcctac ccagttctac cacaagtggc
    20221 aggttaacat ccattatgag ttgcatatta atccgggaag gagcctcttc tttcttttta
    20281 aattcagcaa actccactga ctgagcccta acttcttgtc cttcttttct aggaggggtc
    20341 tcagtcatgg aggcttcctc atctggaaga acagcctctt tctgcttaac agttaaagaa
    20401 gaaactatag ctcgagaaaa agaaaaaggt aaaagttgaa tcatattact atctatagca
    20461 ccatccacta ccaagtggaa aaaaacctcc actaaatgct cttcttcttg ttcttctaac
    20521 cattcctctt cagtactctg cacctcctgt ccaataacct cagggggaga aatgctgatc
    20581 ttccttttca aaaattctga catagcagta gctgctgagc ccatcatctg gttcatagcc
    20641 tctcctaaag cgctgatctc catttctccc agggcctctt ctgcttctgc accacccatc
    20701 attaaactta tcagtgtccg agcatcttct tccttcacca aaagaagatt acttccttga
    20761 agtccctctc gatattgaac tttaaccgca atatgttcct ctggaaaaag gctcgttact
    20821 tcttccttgt ttatcacttc cacccgagga acagtaattt ccactttccg tcctaaaata
    20881 gtggatagag cagtagctgc tgcacccata gaaatatttc ccaattctcc aataacatct
    20941 ttctcctcca gagtcaaagt atcttctggc attcactctt cctcctctaa ttccataatt
    21001 ttaatagcca gccttcgacc tgactttcct ggcattcctt tcatcttcgt tttttttcct
    21061 accagaatat ttaaaggctc tcccgccctt ttatctaaca ccactacatc ccctatttct
    21121 aaactcacaa gttctctcaa gcttatattt gtcctaccca gttctaccac caccggcaag
    21181 cgaacctggt taattttctt tttcaccttc tcctgaatct gggggtcatt agatttacgg
    21241 ctggcaaacc ataaagaggc actcaatctc tgggtgatgg gttctactac caggtaggga
    21301 atacaaatgg tcatcatccc ctcccgttct cccacttcta ccgaaaaagt aaaaagaatt
    21361 atcatatcat taccagaaac tacctggaca aactctaaac ttgattctaa agcttctatc
    21421 tgtggttcca cctgctggat atttgcccag gcttctttta aagcactcat aatcctttct
    21481 gtcacttcca gcataattcg attctcaatt tcagttaaag gacgaggatg aaaaacggta
    21541 cttcctgaac ctcccaataa gcgatctaac atgggaaaaa ccatatccag attcatctgc
    21601 aaaacacacc gtccctctaa agggggaaga gagaaaatac atacaaaagt aggattggga
    21661 actgaacgaa taaattcatc ataagtcagc tggtctactg aaactaattc tacctgacaa
    21721 cggctacgca gataagcagc taaactggta gaggctaaac gtgccagagc tgaaaaaatc
    21781 atatgaaaag tgcgtatctg gtctttagaa aatttctccg gacgtcgaaa gtcataaacc
    21841 ttgaaacgcc cgccctcttt tctctcttct ctaatggctt ctgcttccag gtttcctgag
    21901 tttaaagctt taagcagagc atcaatttct tcttgagata gtatatcact cattttctgc
    21961 tcctactgca taacgaaatc cacaaaataa attgctgtta ctttccccga ggttaaaaac
    22021 ttattaatgc tatcagctat gagtgttttc aatttttctt taccttcggt agtagagagg
    22081 tcttcggagc tttgagcgct gaggatggta attatagcat cccgaatttc cactatctta
    22141 tttttaatct catccaccac tcgctggtta ggaacttcca ctcccagggt tattttgaga
    22201 taacgcaaca catctttatc actcaagtta actagaaaat tttgctcaaa aatataaatg
    22261 aaagaaggag ctgagtttac ttcttgggga gaagaagcaa atagcttact ccatatcccg
    22321 ctctgctgga gataaacaaa aaccatgaaa gccaagagaa ctaaggtaac aaccagtatt
    22381 atcatctgaa aagaaaaacc cttttctttc ttttcttctg aattttcagt tttttccggc
    22441 ataatctttt ctcctttcaa tctcgtaaga taactatttc tactcgccga ttcttctggc
    22501 ggtgttcctc atccaggttg ggaaccagag gtcggtattc tccataacct actgctatta
    22561 atcgctcagg cggtaatcct ttttgctcca ccagataccg caacacagca atagctcgag
    22621 cagcagacag ctcccaatta gaaggaaact gagaggtatg aatgggtaaa tcacaggtat
    22681 gcccttctat cctcaccgag ttttcaacct cctcaagagc gtcagctaaa gcatccaaaa
    22741 taggaaagac tcccggcttt aaatctgctt tccccacatc aaaaaaaacg gtatctaaaa
    22801 aagtaatcac taatcctcgc tcgtctattt tgaaactaac gttatccgga gaaagatgac
    22861 cctcctttat taattgcctc tcaatttctt tcataaggcc agccaacttt aactccacca
    22921 accctgaggg actgatctga gtggggggtt taggaacagg aatatcagaa ggattgagaa
    22981 ctctagggcc accctccagg actcctaacg ctccctgaat agaagaaatt acctccttaa
    23041 atcgggccac atcaatggta gaaagagcaa aaagcatgat aaaaaaacaa agtaataaag
    23101 acataaaatc tccataggtc aaagaccaca gaggcgctcc ggggggcgtt tcttcttccg
    23161 ctcttttacg gggcatctaa ttcacctcat acttcacttt ctctttttaa aggaatcact
    23221 ttacctactt cctccctctc tcgctccagt tcttctctaa tactaggagc aaagaaggac
    23281 ttaagttttt cttccacaat gcgagggtta tctcctgctt gaatggaaag aattccctca
    23341 atgataactt cttttagcaa cacttcttca ctactcctta actttagctt attggctata
    23401 ggcagacaaa tcagattagc aagtatagct ccatagagag tggtaagtag tgctactgcc
    23461 atacctggcc ctactttttt ggggtcatct aaactaacca acatagctac caaaccaatg
    23521 agcgttccaa ttaaaccaaa agacggagca aaagcaccca tagtctcaaa aatagctttc
    23581 ccttttttgt ggcgttcttc cagaaaaatc aactcagtct ccaggatatt tttcaataac
    23641 tgagggtcag ttccatctac taccaattga attccccgtt taaaaaaagg gtcttctatt
    23701 ctctcggttt cttcttccag agctaaaagt ccttctttac gagctttttc tgctaaaccc
    23761 accagagtaa tgattatctc cgaaggttga gggagtcgag aacgcatggc aatcataaac
    23821 agctgaaaag ccttcatcac ctgctccaga tgaaaactaa taagagtagc ggctaaagtt
    23881 cctcctccta caataaggat agaagcaaag tcatagtaaa ttctcatatt attagctgcc
    23941 gcattggtca taccccagaa aagtaaaact actcctacta ccaggccgat tatagttgcc
    24001 ttgtccatag ttactcctcc aactcatttt caggtaaatt ctgaattaat attttactca
    24061 ctccgctctg tcggcgaaaa tctaaaattt ttccgattat ttcatctact gactctttaa
    24121 ccacatattt cctaccggta accagggtta taatcgtatc tggagtagcc tctatggttt
    24181 caatcaaatc catatttaaa acaaaacttg aaccattaaa tcgagttaaa gtaatcatcc
    24241 tctctccctc tcacgcttta gaaaaagagg ggcttgcgcc cctctttttc accgtttgat
    24301 attaattaaa tcttgtaaca tttcatcaga cgtggtaatc actcgagaat tagcttgaaa
    24361 acctcgctga gtaactatca tattggtgaa ttcttgtgct aaatcaacat tagacatctc
    24421 gagcgccccc acggcgatgc ttcctcttcc tccactactg gctgttccga tattgggaag
    24481 accagagtta ttggaaatct ggtacagatt tccaccttct ttcaataaac ctcccgggtt
    24541 agaaaaagtg gctaaagcaa tttgagctaa ggctttagat tgaccattgg taaaaatccc
    24601 ggttaacact ccaccagaat cagtgctcac catatctaaa gacccggcct catacccatc
    24661 ctggaagctg atgtaagcag tggggttacc accaaactga gtaagttgag aaaaatcgat
    24721 gcttaggcca atagaatcag ctccaggaat agaagtaaaa ctagccatac cagtaccacc
    24781 cgagaaaacc gtaccatcag gattaaactc aatagcacca gaatcaccag atattccatc
    24841 taaatccgct gcccaacccc actcatttgc tgttgcagtt ttcgtaaaag ttattgttaa
    24901 gttgtgaggg tttcctaagg aatcataaac ctgaacactg gtaatccatt gagagctatc
    24961 ggcagcgtta gcatctaaat tcccttcaaa ggcaatttct gtggtagctt taggcttcat
    25021 ctgagcaccc accgggatag ttatattctg gagagcttgg gtagaatcga tatttccatc
    25081 ggcatcggcc atccagcctt gcaggcgaaa accgttacta tttaccagcg ctccatcagc
    25141 agaagttttg aaagctccat ctcgggtata atatacttga cttccatcac tcactatgaa
    25201 aaacccctct ccctgcacgg caaaatcggt ggggttatca gtgctctgta aactccctgg
    25261 agtaaaaaga gtatcaatgc tcgagatacc catccccaaa cctacttgtt tggggtttac
    25321 tcctcccatt cctccggttt gaggactgcg agctccttct acagtctggc ttaagatatc
    25381 ttcaaaattt actcgaccag atttaaaagc tacggtatta acattggcaa tattatttcc
    25441 tattacatcc atccgggtct ggtgattttt aagacccgaa actccggaaa acaaagagcg
    25501 catcatctta atttacctcc tttcggggag cccccctaag gaccagctcc ctaagcaata
    25561 atcgctgaat caatattggt aaagatatta tcttttcgag aattagaatc catacaggtt
    25621 accaccactc gctccgggac attcactaca aaagccactt ctcccactaa aaccagagag
    25681 tttcttcctc ccttttcttt tactttctgt actgcctctt gcagacgctc tacggtatcg
    25741 ggagaaagag gtatctctct ctcttttatt cttctttgag catggagaga aaaactcaac
    25801 tcctcttctt gcagaacttg ttcaaagttc gcagtagttt ttgccttctc ccgaagagga
    25861 gtagaaggtt gaatcccagg aatgaattct cgaaaatttt ctatcttttg aaccatcagc
    25921 tctctcctac ccttaccact tcgctaatat tgtact
//
LOCUS       DAOSVE010000016        25439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_1274_length_25439_cov_68.827608, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000016 DAOSVE010000000
VERSION     DAOSVE010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 25439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25439
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1274_length_25439_cov_68.827608"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..1073
                     /gene="folD"
                     /locus_tag="PK016_02535"
     CDS             201..1073
                     /gene="folD"
                     /locus_tag="PK016_02535"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HXL01984.1"
                     /translation="MSAQILDGKKISAEVREELKPRIAYLSERGCQPGVAVVLVGDDP
                     ASQIYVRNKEKACEKLGIYSIQHRLPADTSQEEVIELVNSLNENERIHGILVQLPLPS
                     HIREKEVLYHISPDKDVDGFHPYNLGRLMIGDPIFLPCTPWGVQELLLRYNLSPEGKN
                     VVIVGRSNIVGKPLSMMLVQKGKGANATVSVCHTGTRNLSSYTKMADILIAACGSPHL
                     ITQDMVREEAVVVDVGINRVGDKTVGDVDFERVKEVASYISPVPGGVGPMTIAMLMAN
                     TVKAAERALQRKTA"
     gene            1153..1647
                     /locus_tag="PK016_02540"
     CDS             1153..1647
                     /locus_tag="PK016_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HXL01985.1"
                     /translation="MKIAVISDTHIPQRGRNLPDYLLHFLGGVSLILHAGDITEWWLL
                     EELMKYAPVQAVRGNMDSPLVRERLSDKEILEIEGVKIGIAHGEGSPAGIEERVGKLF
                     NGENLQVIVYGHTHQPSKEWRGEVLYFNPGSPTDWVFAPFFSFGFLEVREGKIIEAEI
                     LKVD"
     gene            1658..3226
                     /locus_tag="PK016_02545"
     CDS             1658..3226
                     /locus_tag="PK016_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HXL01986.1"
                     /translation="MAEKLMVNITWHMHQPFYWNEEKGVFGFPWVRTHATKDYLFMGK
                     LVERFPSLRVTFNFAPSLLRQFQLYLEGKEDMVMKLAKKEASTISEEDKKLIRENFFV
                     VSSPRTFEKWPRFAELREKPWESFTSQDFLDLQILYQLIWFDPLWREENPVGRELWER
                     GRNYREEDKKKIWKATEEIFREIFTQYRRLLEKKQIEISFSPFYHPILPLLIDTDVAR
                     ESNPSTILPHRFSFPEDAQEQIKRGKSFSQDIWGSDFMGMWPSEGAVSEEAIRLAIQE
                     GVSWLATGEEILFRSLGKSKEVAWQELYLPHVWGREGRNIVIFFRDTGLSDLIGFSYQ
                     YFPPQEAVEDFINHLRAIKKALPENKPYLVNIILDGENAWEYYIDNGLPFITALYQAL
                     SESEEFVTITPLEYLQKFSHLPQISHIVPGSWICGNLNTWIGHPEKNWAWEKLRRVRE
                     LWSREKGYLSKEEEERIQDLIYQAEGSDWFWWLGDDNPSSQKENFRQHFLCLIHGILR
                     TLTKEERPCIKEKF"
     gene            3205..4488
                     /locus_tag="PK016_02550"
     CDS             3205..4488
                     /locus_tag="PK016_02550"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HXL01987.1"
                     /translation="MYKREVLTLILAGGEGKRLDILSEKRAKPAVPFGGKYRIIDFPL
                     SNCVNSGLYCVGIPTQYNPRSLHEHIGIGKFWDLDRMEGGVFLLQPYISDVVTNWYRG
                     TADAIYQNLRFIRDQDPRLVLILSGDHVYKMDYRNLIRFHQERKADLTVAVMEVPWEE
                     AHRFGVLSLDEQGRVTDFEEKPPHPHSNLINMGIYIFNREVLEEEVEEEALREDTSFD
                     FGKDIIPRMVSQRKVYGFCFSGYWKDVGTIDAYWEANMELLGSSPALDLYDKQWPIYT
                     FIEDRPPVKLSHRAKIKRSLLGSGAIIDGSVEDSVIFSGVYVSRGAQVRHSIVMSDTL
                     INEEAVVDRAILDKRIVVGRRAKVGYGEELRPNRKIPDMLRSGLTIVGRNAHIPPEAI
                     VGRNCRIGTDVGEEDFPSLILESGESIEKAEEGWK"
     gene            4485..6008
                     /locus_tag="PK016_02555"
     CDS             4485..6008
                     /locus_tag="PK016_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01988.1"
                     /translation="MKKTWVVLLLIFFLWSGWAGAQTFEFDVSSSEASQIDSLPLSPF
                     IFLLSDRGEWGLYYPGESIILQYGSSRDGYVSIFDYTPDGKARIIRNNEPIAEGVQRK
                     IYGVVSGPEGIERFLMILSEQVIPDRLLIEIMRNPSRIEGILGYSLPLHYCRIQVATK
                     ERTLSPSFISFDQVPSEIPAGGKLKVRAFLSDGEGNALFNRPIYWEVNQGTLDNYEIY
                     TSTSGVAEVWYSAPSLSDSTEVILKAHFPGDMVYQEAEGEIHFLVNPEKIQTTIALTP
                     KSFHIAGGETLDFQAELRDLRGRPVEGRSIRWETSVGSWEKEITYTDSAGITTNRLYA
                     PKVAGQEMVEVKASFAGARNLLPSEDYAYGTISGLEVYPKEGLFFLDFTSGEPYTNFD
                     HLVYRGELLSGYAVNPAWVLSVSGGEFVEVGFSLSQPLRFGALYLWGKAQDEVFLKIF
                     LNDQLIFSGRAAEGTIAPAEAQMIYIADYLKLGNNLLRIEIDSSWGLYYLQRLLVAF"
     gene            6018..6455
                     /locus_tag="PK016_02560"
     CDS             6018..6455
                     /locus_tag="PK016_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXL01989.1"
                     /translation="MKKDFNDWWEEINYFQEEMQKFVKHISEGKAGFTEFGEEIWIPS
                     CDIFETSHHVVIVVELAGVSPQEVEVLIEGKKLIVKGQRKELPYSSKENYHLMEIRFG
                     PFYREISLPEEIGEVKAKYSNGFLVIEGLKNVRKRKISLTQEI"
     gene            6459..8897
                     /gene="lon"
                     /locus_tag="PK016_02565"
     CDS             6459..8897
                     /gene="lon"
                     /locus_tag="PK016_02565"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HXL01990.1"
                     /translation="MEWDNAWFVLEEGLMGEENVSLSGDSPSSEENKENEFPEEIPIL
                     PLKDNVLFPEIALPWIVTGEPWVKLINDAVLDKKILGVVSLRKEARGEELSPADFYEI
                     GVIGKISRLIKLPDDAVQVLIFGLVKFRIKEWVKWEPYPVAKIEIIKEEETHSDEVEA
                     LVRNVVSLFQQVVELAPYLPREAFITALNIKKPSRLANFIASNLNLDVENKIELLSAP
                     DLSTKLTRLSFYLTRELEILQIGSRIQSQVQQEMAKTQREYFLREQLKAIQQELGELD
                     ERSSEIKELKEKLESLDLPPEVRKEAEKEIERLSRLSPNSFEYPVIRNYLDWIIELPW
                     KVSTEDNLDIDLARKILDEDHHDLEKVKERILEYLAVYKLKKDIKGPILCFVGPPGVG
                     KTSLGKSIARAMGRKFVRISLGGIRDEAEIRGHRRTYVGALPGRIIQGIRKSGTNNPV
                     FMLDEVDKMGMDFRGDPASALLEVLDPEQNSNFVDHYLGVPFDLSRVMFIATANVLDT
                     IPLPLLDRMEVIFLSGYTDLEKIAIAQRYLIPKQCEQNGLQPQNVIIEADIIQKIIRE
                     YTREAGVRNLERNIATIFRKIAKEVAQGVQGPFVVDEEKLREFLGPRRFLDEVMEGKD
                     EIGVATGLAWTESGGEILFVETVVLPGKGSLLLTGNMGEIMQESAKIALSCVRARAGE
                     WNIPPDFYEKNDIHVHVPAGAIPKDGPSAGITMVVSMISALAQIPVRHDVAMTGEITL
                     TGKVLPIGGVKEKVLAAYRAGIRKVILPRENEKDLQEVPEEVKKTMNFSLVENINQVL
                     DLALVKEELVEVKN"
     gene            8913..9971
                     /locus_tag="PK016_02570"
     CDS             8913..9971
                     /locus_tag="PK016_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HXL01991.1"
                     /translation="MEKDRIDFLRNLIDTPSPSGFEEKVQGVFLERIKDRVDQYYKDV
                     HGNAFGVVNPEGKIKVMLAGHCDEIGMMVEYVNDQGYIYFSTVGGIDSHITPGKQVLI
                     HTSGGVIPGVIGKKPIHLLEEKERQKVAKIEEQWIDIGAQSKEEALKQVTVGDPITFD
                     AGFKSLLGTRVVGKGFDDKVGAFIVGEVLRCLEKDKLHCAVYGVSTVQEELGLRGART
                     SAFGIAPEIGIAIDVTFASDCPDIDKRKVGEVALGKGPVISRGPNINPRIWQRMINIA
                     KEGDIPYQVQAESRATGTDANAIQITREGVATGLIGIPNRYMHTPGEVVDLEDLEHAI
                     ELLVRFLHSLEGNEEWIP"
     gene            9993..11006
                     /gene="galT"
                     /locus_tag="PK016_02575"
     CDS             9993..11006
                     /gene="galT"
                     /locus_tag="PK016_02575"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015920093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HXL01992.1"
                     /translation="MPEFRKDPLFGRWVIIAEERGTKPYQFGEGWERESSFPCVFCPG
                     RESYTPPEVFSIREKDTPPNERGWRVRVVPNRFPALLPGEPLRQYVDGFYERMSGFGV
                     HEVIIETPDHYSDLVDLPLKQVEEVLWVYRERMRELGEDSRLRYCLIFKNQGEKAGAS
                     LPHAHSQLIATPVIPKKVQEELKGAREFYEEEGNCFFCSLLEEERKRERLVWEEERFI
                     AFVPYAARFPYETWIFPQRHCAFFSEIEEEEIRNLAYVLKGVLSAIKTLLHDPAFNFI
                     LHAAPYSRSLENYADYYHWHMEILPSLGKMAGFEWGTGFYINSVIPEKAAKALRQFIK
                     DKN"
     gene            11016..12269
                     /gene="glgD"
                     /locus_tag="PK016_02580"
     CDS             11016..12269
                     /gene="glgD"
                     /locus_tag="PK016_02580"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HXL01993.1"
                     /translation="MSIKYAMILAGGKGDRLSVLSVDRAKAAVPFGGCYRLIDFPLSN
                     CVNSGIYDVGILTQYQPRSLIEHIGVGRPWDLDRKKGGIEFLQPYLSRTAKGWYRGTA
                     DAIYQNLNILSRKKIAHLLVLSGDHVYLMDYNPLFSYHLERDADVTLVATRVDKKDAP
                     RFGIMEVDENNWITGFEEKPFSPRTDIAFMGIYIFRMEFLVEKLINNAQEGKFDLVRN
                     VIIENVGKAKMQAYFYNDCWFDVGTVKAYWEANMHLLEPLPAFNLYNPDWMVFTNRGH
                     YPPTHTLEKADISSSIIGEGAVIKGKVVHSVIFPGVVLEEGTEVIDSIVFNNTVVKEG
                     AKVNLSILDKNIVVGQDVIIGYGEDFTPNKARPELLDWGVNVVGKGSIIPARMVIHRN
                     CLIGIGIKEEHFGERKELKSGEVLL"
     gene            12291..12542
                     /locus_tag="PK016_02585"
     CDS             12291..12542
                     /locus_tag="PK016_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL01994.1"
                     /translation="MLLGILLIVVVLYGLLLFWILNERLKDLDYSVNKVQESLKEMDN
                     KLVLLLNLKSEEEEKDSALEETFSLHILQGETKKKGDKS"
     gene            12542..13477
                     /locus_tag="PK016_02590"
     CDS             12542..13477
                     /locus_tag="PK016_02590"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXL01995.1"
                     /translation="MKKQAIFWSVVLILLLSLGWGVFAQEVQETQQEVQEIQEESSKK
                     TVVAEVNGEPIYLEDVQEIWDTLPEVYRIQFPGGIQDILEQLIRETLLVQQAQKLGLE
                     NDPVILEQLEQIKRQLLVTELIKREIVDKVQVSDEEIEKEYTSNPSLYTEPEQVKAKH
                     IMTSTKEKADEVLQELQSGRPFEEVAKEKSESPDAQQGGEMGYLKKGDLDPNLEKVLF
                     DLAPGKFSDVVETSYGFHIFLVEEHLKPRLKEVEEVKDEISNRLLPRKQQEAFEKLIE
                     ELKSQTEIAIIEENLPKEATPQPEASPETGGESQP"
     gene            13474..14850
                     /locus_tag="PK016_02595"
     CDS             13474..14850
                     /locus_tag="PK016_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HXL01996.1"
                     /translation="MMEVLEKLIRLPQKGEYMEVRLESIFSTTIRVQNGVVEKIRVGT
                     DEGGYIRLLAPRGSWWFESFQHWDGLQEKVERAINGASLLPKSDIEVIRKPPVEDEKK
                     VTLTDDFRFKSLKDKTSLLLSYDRILREISPLLVSSLVEYRDEFRQIYLANSDGSLIY
                     WEKPDISISFMATARKGDNIEVYRNGVAGKAGFELVKNQETLAQEVGGKALLLLEAPS
                     FSGGVYDVILDPVLAGVFIHEAFGHLSESDTLYHNEELKKIMAKGKKVSSPILNVFDD
                     GSLDNLRGSSPYDDEGIPTGRTYLIKDGYLSGRLHSRETAFLMQEETTGNARTINYGF
                     PPIVRMTNTAIEEGKTTREDLFSDIERGLYAVEYIGGNTALELFTFSAAYGYLIEKGK
                     IKEPVKDVVLSGNLFQTLDKIDAVANDFRWTEVGGCGKGGQMGLPTPTGSPHIRLREV
                     LVGGSHND"
     gene            14843..16147
                     /locus_tag="PK016_02600"
     CDS             14843..16147
                     /locus_tag="PK016_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HXL01997.1"
                     /translation="MIEVASLLKKRGIPGDIFAHQIFNQEVSYEANLFKGYRKEEREG
                     YGLRVINPEGKLGFYAFNRLLNPQEALDKALEVSQLGDRVDFSIPTYPSSWEWENFVD
                     PRVENLEAQEMIELGDYILSRIREEYPRVLVDIVISAGKEEKSLLNHHEEELKFARTF
                     FEIGVSANQTREGDILEVYTERSWGNRDIDVEGLVQEIVSKLEFSQRVANLNSGKYPV
                     VFTPSGSLVLFIALFYGLNGKNWVFGRSPLGDKEGKVLFSSLFSLEEIQEPWALGSCP
                     FDDEGIMTKAQRSIIDRGRVESAFWDLWSAAKKGIKPTGNGFRSSYRDLPEPGFSSLR
                     VKNGLRDRDTLIGELEEGLIVDSVLGLGQSNVASGYFSCNVQLGFYVKKGEIQGRVKD
                     VMISGNAYPALFNLKEISRDSRWIGGRILLPYLLIEPVEVNK"
     gene            16196..19348
                     /gene="ileS"
                     /locus_tag="PK016_02605"
     CDS             16196..19348
                     /gene="ileS"
                     /locus_tag="PK016_02605"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HXL01998.1"
                     /translation="MFKALGKLSDLAQQEEEILKFWKEKDIFKKSLKQREGSSRFTFY
                     EGPPTTNGYPHAGHIIGRSIKDLIPRYRTMCGYYVPRRAGWDTHGLPVELEVEKELGI
                     SGKPDIERYGVDKFNEKCKESVFKYIKEWEKLTERLGIWMDMEHPYITCSNDYIETLW
                     WVLKELWERKLLYKGHKVLPYCARCGTSLSSHEVALGYRDTEDPSVYVKFALQNREKT
                     YFLVWTTTPWTLVANVALAVGKGIDYLEVVNEENGEHYILGEDSFYRLLGEDANYKIV
                     RKYKGEELTGIKYEPLLDFLVADKGYEVFSADFVTTEEGTGVVHIAPAFGEDDLQLAQ
                     REGLPLLQPVKRDGKYGEEITPWQGMWVKDADPLIIDYLKEKGKLFKQEIYRHSYPFC
                     WRCDTPLLYYAKESWFIKTTAFRDSLLENNQKINWYPPHIKEGRFGDFLSNVVDWALS
                     RERYWGTPLNIWQCESCDFQEAVGSIEELKSRSREKIEEIELHRPYVDGVTLSCPRCG
                     GAMKRVPEVIDCWFDSGAMFVAQLHFPFENKEEFQQSFPADFVCEAIDQTRGWFYSLH
                     VLATVLFNSPAYRNCLVTELGLDEKGQKMSKHIGNVVNPWELIENYGADVLRWYVFSV
                     SPPWVPKRFGKEAIRGVLSKFFDTLWNVTNFFILYANIDAFEPPISFLPLEKRSAFDR
                     WIISCLEELIKEVRSSLDHYEVSRASKAIEDFVIEDLSNWYIRRSRRRFWKAEKDEDK
                     ISAYHTLYYVLTNLTRLLAPFIPATAELLYQILEADVQGKKESVHLENYPLPEEKNID
                     RELILAMEVVRKLTNLGRSVRNKVNIKLRQPLRKAILVVPQARERDRALPFASIIQEE
                     LNVREIEWISHLPEEVTVSLKPRFSILGPRYGQKVKEIARLLKNLEEDKSRQFWEEGE
                     VSLLLEGEEIILKREEVELELQTREGLAVESEGEYAVILDTFLDQGLQEEGMVRDLIH
                     AIQVWRKEAGLEVEDRIELQVREGTDPEWRDLIEKYSSLIQSEVLASSLSLSSNGGGE
                     MTSREWELSGKKLALALRRK"
     gene            19351..20481
                     /locus_tag="PK016_02610"
     CDS             19351..20481
                     /locus_tag="PK016_02610"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HXL01999.1"
                     /translation="MESKRRLLFLFLFLVSTWVFPAWADSYHVVESGDTLWAISRRYQ
                     IPLEKLLQINGLTEKSTLRIGQKIKLEEGANEAIYQIKAGDTLWGISRQFNIPLSSLL
                     AANGLTEKSILRVGQKIILPSSSKEEAKAVVTSSNVTHIVKKGESLWLISRLYGVDMD
                     SIMKANKLTADSILSIGMKLTIPQVRVASSPVKEDNFEVYRVQSGDTLWDISRRYRVS
                     LQDLMKVNNLNEKSTLQIGQSIRIPSSLPKNYEKASSSFLWPVSGRVSSTFGMRGGRL
                     HSGVDVLAPSGTLIKASRSGVVSFSGWMNGYGRVVIVDHQDGWQTLYAHNSVNLVNKG
                     QKVNQGDAIARVGMTGNATTCHVHFEVRKNGKCLDPLNYLGK"
     gene            20483..21037
                     /locus_tag="PK016_02615"
     CDS             20483..21037
                     /locus_tag="PK016_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HXL02000.1"
                     /translation="MEREEAWTLLKKHLKNRNLLKHSIACEVIMQFLARQWGENEKEW
                     ALAGLLHDIDYEETNGELTEHGRLGAEILKEAGLPEEIIRTVQAHNPEATGIKPESLM
                     EKALYAVDPVSGFIVACALIHPEKKLDSLDLSFLLHRFSEKSFARGANREAMKSIEST
                     GLSLEEFLKIALSAMQTKKEELGL"
     gene            21132..21566
                     /locus_tag="PK016_02620"
     CDS             21132..21566
                     /locus_tag="PK016_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXL02001.1"
                     /translation="MAINRWDPFRDLAELQGEIDRVLGSTFSRRGKETTVGSWLPPVD
                     VKEEEEEFVLFVDLPGVDEKAVEITVVGDQLTIKGERKAPEEGENFLRQERVFGPFHR
                     SFLLRMPVEVDKVSATYKNGVLEIHLPKSSAAKPRKVSVQVE"
     gene            21622..22668
                     /locus_tag="PK016_02625"
     CDS             21622..22668
                     /locus_tag="PK016_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HXL02002.1"
                     /translation="MEFKDYYQILGVPRGASEKEIKKAYRRLARQYHPDLNPGNKEAE
                     KKFKEVNEAYEVLSDPEKRKRYDELGSAWNNYGRQNTDEFWSDFYRRYGGGPAYTTQE
                     EEFEAGDFSDFFKTIFGDLLGGTRQSSRGRGFRFHTQGEGIRREPPPEVQSEVEISFE
                     ESFRGTDRNIQVEYEELCSNCGGTGRNSEGKLCRSCGGRGKEKKSRKVNVAIPPGVSD
                     GTKLRIPQILGGRDLYLKVRVKPHPFFRREGDNVILELPVTIYEATLGTEVEVPTLEG
                     KAKMKIPPETQNGTIFRLRGLGFPRTKEGGRGDQLVKIKVVVPRGLTERERKLLEELA
                     SLRKENPRQHLVRI"
     gene            22680..23036
                     /locus_tag="PK016_02630"
     CDS             22680..23036
                     /locus_tag="PK016_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HXL02003.1"
                     /translation="MEKEKKPNGYFLIKEVANITQLHPQTLRYYEKIGLVKPHRSQGN
                     ARLYSLEDIERIKKIKVYTRDLGVNLAGVEVIFKLTEQIEELQKEKDKIKEQLEVKNN
                     KMDTEPPVKERRRKIK"
     gene            23033..>25439
                     /locus_tag="PK016_02635"
     CDS             23033..>25439
                     /locus_tag="PK016_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXL02004.1"
                     /translation="MRFEQFTEKAKEVISLAQDILLEYHHNQLDVEHLLLALLKQKEG
                     LAGQILEKCKVDPQIVAREVEEALQRFPQVYYQGGEEMQIYLTPRAKSVLDNAEEEAQ
                     RLKDSYVGVEHLLIAVVREESGASSRILKSHGLDVEKIYQALQEIRGKRRVDDPYAEG
                     KYMALEKYGRDLTQLAREGKLDPVIGRDEEIKRVIQILSRRTKNNPVLIGDPGVGKTA
                     IVEGLAQKIVKGDVPESLKGKRLIALDMGALLAGAKFRGEFEERLKAVLEEVQKSEGK
                     IILFIDELHTVVGAGAAEGAIDAANILKPALARGELRCIGATTLDEYRKYIEKDSALE
                     RRFQPVYIGEPSIEETKEILKGLRDKYEAHHRVKISDNAIEAASELSSKYITDRFLPD
                     KAIDLMDEAASKKRIEIESVPSEIKTLEEELQKLAKEGMAAVQAQEYEKAAALRDKAE
                     ALQKKYQEEKEKWMKEREISEVVGEEDIAQIVSSWTGIPVAKLVEKEKDRLLHMEEEL
                     HKRVVGQDDAVRAIAEAIRRSRAGITEGRRPIGSFLFLGPTGVGKTELAKSLADFLFG
                     DENAILRIDMSEYMEKHSVSRLIGAPPGYVGYEEGGQLTEVVRRRPYQVILFDEIEKA
                     HPDVFNILLQILDDGRLTDGQGRTVDFTNTVIIMTSNLGSSYFRGIDPEKDRESLRRA
                     KEKVLEEVRNHFKPEFLNRIDEIIVFNPLSKEEIKKIVDLMINRLRNRLQVQGMDIVL
                     TQEVKNYLAQTGYDPDYGARPLRRLIQREIESPLSGELIRGTFQPGDVIEVYLEEGEI
                     KFRR"
BASE COUNT         7921 a         4075 c         6209 g         7234 t
ORIGIN      
        1 gaattctttc ccccttatac tgaggaaagc gtttttcttt cctcagtagt ttttgtcttt
       61 tcagttttta attttttgct tttgggggtt atagggggat cattttcccc cctatagtcg
      121 ggattataaa ggggtgctcc ctctcatagt cattataatt agttttgcgg ttaactataa
      181 atagaaagag gaggtaaaag gtgagtgctc aaattttaga tggaaaaaaa atttcggcag
      241 aggttagaga agagcttaaa cctcgtattg cctatcttag tgagcggggt tgccagcctg
      301 gtgtggcggt agttttagtg ggggatgacc cggcaagcca aatatacgtg cggaacaaag
      361 aaaaagcttg cgaaaaactg ggtatttatt ccattcaaca tcgcttacct gctgatactt
      421 ctcaggaaga ggtaatagaa ttagtgaata gtttgaacga aaatgaacgc atccacggga
      481 tattagtgca acttcctctt ccttctcaca tccgggaaaa agaagtttta tatcatattt
      541 ctccagacaa agacgtagac ggttttcatc cttataatct gggaagattg atgattggcg
      601 acccgatttt tcttccctgc actccctggg gggtgcaaga attgctatta cgttataatt
      661 tgtctccaga ggggaaaaat gtagtaattg tgggtaggag caatattgta ggtaaaccgc
      721 tatctatgat gttagtccaa aaaggtaaag gagctaatgc tactgtttct gtgtgccaca
      781 ccggcacccg taatctttct agctacacca aaatggctga tattttgatt gctgcttgtg
      841 gctctcctca tttaattact caggatatgg taagagagga ggcagtggta gtagatgtgg
      901 gaatcaatag agtgggagat aaaacagtag gggatgtaga ttttgagaga gtgaaagaag
      961 tagcgtctta tatcagtcca gttcctggag gagtaggccc tatgactatt gccatgctta
     1021 tggctaatac ggttaaagca gcagaaagag ctttacagcg gaaaactgct taaagtaagg
     1081 agtaatgtta aataagagag aaatgtttta gaaaaagcaa aaccgtgata aaattaaaaa
     1141 tggtgaaagg gaatgaaaat agcagtgatt tctgatactc atatccccca gcgagggaga
     1201 aatctccccg actatttgct tcatttttta ggaggagttt ctctcattct tcatgctgga
     1261 gatataaccg aatggtggtt gttggaagag ttaatgaagt atgccccggt gcaagcagta
     1321 agaggaaata tggattctcc tctggtccgg gagagacttt ctgataaaga aattctggaa
     1381 attgaaggag ttaaaatagg aattgctcat ggagaagggt ctcctgcggg gatagaagag
     1441 cgagtgggaa agttattcaa tggggaaaat ctccaagtaa tagtatacgg tcatactcat
     1501 caacccagta aggaatggcg aggagaagta ttatacttta atccgggaag tcctactgac
     1561 tgggtatttg cccctttttt ctcttttggt tttttggaag tgagagaggg gaaaataatc
     1621 gaagcagaga tattaaaggt ggattgaggt gttataaatg gcggaaaaat tgatggtcaa
     1681 cattacctgg catatgcatc agccctttta ttggaatgaa gaaaaaggag tttttggttt
     1741 tccctgggtt agaactcatg ctaccaagga ttaccttttt atgggtaaat tggtggaaag
     1801 gtttccttcc ctccgagtta cctttaattt tgctccttcc ttgctccggc agtttcaact
     1861 ttatctggaa ggtaaagaag atatggtgat gaaattagct aaaaaggaag cctctaccat
     1921 tagcgaggaa gataaaaaat taatacgaga gaatttcttt gtagtttctt ctcctcgtac
     1981 ttttgagaaa tggccccgtt ttgccgagtt aagagaaaaa ccctgggagt cctttacctc
     2041 tcaagatttt ttagaccttc agattctcta tcaacttatc tggtttgacc ctttatggcg
     2101 agaagaaaat ccggtaggtc gagaattatg ggaaaggggt aggaattata gagaagaaga
     2161 taagaaaaaa atatggaagg ctactgaaga aatatttaga gagattttta cccagtaccg
     2221 aagattgtta gaaaaaaaac agatagaaat ttccttttct cccttttatc atcctatttt
     2281 acctcttctt atagatacag acgtagctcg ggaatctaat ccttctacaa ttttacctca
     2341 tcgtttctcc ttccccgaag atgctcaaga gcaaataaag agaggcaaaa gtttctctca
     2401 agatatatgg ggttctgatt ttatggggat gtggccttct gaaggagcag tgagcgagga
     2461 agctatccgt ttggctatcc aggaaggagt aagctggtta gccacaggag aagagatttt
     2521 gtttcgttct ctaggaaaga gcaaagaagt agcctggcaa gaactttatc ttcctcatgt
     2581 ctggggtaga gaaggaagaa atatagttat atttttccgg gataccggct tatcagactt
     2641 aatcggattc agctatcagt atttcccccc tcaagaagca gtggaagatt ttatcaatca
     2701 cttgagagct ataaaaaaag cactgcctga aaataaacct tatctggtaa atatcatttt
     2761 agatggagaa aatgcctggg aatactacat agataatgga ttgcctttca ttactgctct
     2821 gtaccaggcg ctttctgaaa gcgaagagtt tgtaactatt actcctcttg agtatttgca
     2881 aaaattttct catcttccgc aaatttctca tattgtacct ggctcctgga tttgtggtaa
     2941 tctaaatacc tggatcggac acccggaaaa aaactgggcc tgggaaaaac ttcggcgggt
     3001 gagggaactc tggagcaggg agaagggata tttatcaaaa gaagaagagg agagaatcca
     3061 ggatctcata tatcaggcgg aaggcagcga ctggttctgg tggttaggag atgataatcc
     3121 ttcatctcaa aaagaaaact ttcgccagca ttttctctgt ttgatacacg gtattttacg
     3181 tactttaaca aaggaggagc ggccgtgtat aaaagagaag ttttaactct gattttagcc
     3241 ggaggagagg gtaagagatt agatattctc tcggaaaaga gagctaaacc agcggtacct
     3301 ttcgggggta aataccgaat tattgatttt cctttgagta attgtgtcaa ttctggtctg
     3361 tattgtgtag gaattccgac tcagtataat cctcgttctc tacacgaaca cattggaatt
     3421 ggtaagttct gggatttaga ccgaatggaa ggtggagttt ttctcctcca gccttatatc
     3481 agtgatgttg ttactaattg gtatcgagga acagcagatg ctatatatca gaatctccgt
     3541 tttattcgag accaggaccc tcgtctggtg ctgattctat ctggagacca tgtatacaaa
     3601 atggactatc gtaacctgat tcgttttcac caggaaagga aagcagattt aactgtagcg
     3661 gtgatggagg ttccctggga agaagctcat cgttttggag tgttatctct tgatgagcaa
     3721 ggaagggtaa ctgattttga agaaaagcct cctcatcctc atagtaactt aataaatatg
     3781 ggaatatata ttttcaatcg tgaagtcctt gaagaagagg tagaagaaga agctctccgg
     3841 gaggacacct cttttgattt tggtaaagat atcattccta gaatggtgag ccaaaggaaa
     3901 gtttatggat tctgtttttc tggttactgg aaagatgtag ggactataga tgcttattgg
     3961 gaagctaata tggaattact tggatcttcg ccagctcttg acctttatga taaacaatgg
     4021 cctatatata ctttcatcga agaccgccca ccggtaaaac tgagtcatcg agcaaaaatt
     4081 aaacgcagtt tgttaggtag cggagccatc atagatggaa gcgtggagga ttcggtaatc
     4141 tttagcggag tttatgttag tcgaggtgca caagttcgcc attctattgt aatgagtgat
     4201 actttaataa atgaagaagc agtggtggac cgggctattt tagataaacg tatagtggtg
     4261 ggcaggagag ctaaggtagg atatggcgaa gaattgcgcc ctaatcgaaa aattcccgat
     4321 atgcttagaa gcggtttgac tatagtaggg agaaatgctc atattcctcc tgaagcgata
     4381 gtaggtagaa attgtcgtat tggaacagat gttggcgaag aagatttccc ctctcttatt
     4441 ttagagagtg gtgaaagtat agaaaaagct gaggagggat ggaaatgaaa aaaacctggg
     4501 tagttctact acttatcttt ttcctgtgga gtggctgggc tggggctcaa acttttgaat
     4561 ttgatgtttc ttcttctgaa gcaagtcaga ttgattctct gcctctttct cctttcatat
     4621 ttttgctttc tgatcgagga gaatggggtc tttattatcc tggagaaagt attatcttgc
     4681 aatatggtag ttccagagat ggctatgtca gtatattcga ttatactccg gatggtaaag
     4741 cacgtattat cagaaacaat gagccaatag cggaaggagt gcaacgaaaa atttatggag
     4801 tagtatctgg accagaagga atagaaagat tcttaatgat actttctgaa caagtaatac
     4861 cagaccgctt gctgatagag attatgagaa atccttcccg tatagaagga attctgggat
     4921 actcattacc cttgcactat tgtaggattc aagtagcaac taaagaaaga acattatctc
     4981 cttcttttat ttcttttgac caggtgccca gtgaaattcc tgcgggaggg aaactgaaag
     5041 tacgagcctt tttgtctgat ggggaaggaa atgccttgtt taatcgacca atttactggg
     5101 aagtcaatca aggaactctg gataattacg agatttatac ttctacctca ggtgtagccg
     5161 aggtctggta cagtgctcct tctttatcag atagcacaga agttatatta aaggcccatt
     5221 ttcctggaga tatggtttat caagaagcgg aaggagagat tcattttctg gtgaatccgg
     5281 aaaaaataca aactactatt gcccttactc ctaaatcttt tcatatagct ggaggggaga
     5341 ctcttgattt tcaggcagag ctccgggatt tgcgaggaag accggtggaa ggaagaagta
     5401 tcaggtggga aacaagcgtt ggtagctggg aaaaagagat cacttatacc gatagcgcag
     5461 gtataactac taatcgttta tatgctccta aagtagcagg acaagaaatg gtggaggtta
     5521 aagcttcttt tgccggagca agaaatcttc ttccctcaga agattatgct tatgggacga
     5581 taagcggttt ggaagtttat cctaaggaag ggctattttt cctcgatttt acttccgggg
     5641 agccttatac taattttgac catttagttt atcgagggga attgctctct ggctatgcag
     5701 ttaatcctgc ctgggttctc tctgtgagtg gtggagaatt tgtggaagta gggttttctc
     5761 tttctcagcc tctccgtttc ggagctttat atctctgggg taaggctcaa gatgaggtgt
     5821 ttctcaaaat ttttctcaat gaccagctga ttttttcagg tagagctgca gaaggaacaa
     5881 ttgctcctgc tgaggctcaa atgatttaca ttgccgatta tttgaagtta ggtaataatc
     5941 tattgcggat agaaatagat tcttcttggg gattgtatta tttacagaga ttattggtgg
     6001 cattttagga ggaaattatg aaaaaagact ttaatgattg gtgggaagaa attaactact
     6061 ttcaagaaga aatgcaaaaa tttgtaaaac atatctctga agggaaagca ggctttactg
     6121 aatttgggga agaaatctgg ataccctcct gtgatatttt tgaaacctct caccatgtgg
     6181 taatagtagt agaactggct ggtgtttctc ctcaagaggt agaagtattg atagaaggga
     6241 aaaaacttat tgtgaagggg caaaggaaag agctgcctta ttcctctaag gagaattatc
     6301 atttgatgga gattcgcttt ggtcctttct atcgagaaat tagcttacca gaggagatag
     6361 gggaagttaa agctaaatat tccaatggtt ttttagttat tgaaggatta aaaaatgttc
     6421 gaaaaagaaa aatttccttg actcaggaga tatagaagat ggaatgggat aatgcctggt
     6481 ttgttctgga agagggattg atgggagaag agaatgtgag tctttcaggg gattctcctt
     6541 cttcagagga aaataaagaa aacgaattcc ctgaggaaat acctatttta cctttaaaag
     6601 ataacgttct tttccccgag attgccttac cctggatagt aacgggagaa ccatgggtaa
     6661 agctgataaa tgacgcagtt ttagataaaa aaatcctggg ggtagtttca ctgcggaaag
     6721 aagccagagg agaagaactt tctccggcag atttttatga aataggagtg atagggaaaa
     6781 tttctcgtct tatcaagctt cctgatgacg cagtacaggt tttaattttt ggtctggtta
     6841 aatttcgaat caaggaatgg gtaaagtggg agccttaccc agtggctaag atagaaataa
     6901 taaaagaaga agaaactcat tctgatgagg tagaagctct ggtaagaaat gtagtgagtc
     6961 ttttccagca ggtagtagaa ttagctcctt atcttccccg tgaagctttt ataacagctc
     7021 taaatattaa aaagccctct cgtttagcta attttatagc ttccaacctt aacttagatg
     7081 tagaaaacaa gatagaactt ctatctgctc ctgatttatc taccaagctt accaggttaa
     7141 gcttttacct tactcgagaa ttggaaatac tgcagattgg tagtcgaatc caatctcaag
     7201 ttcaacagga aatggctaaa actcaaaggg agtatttctt acgagagcag ttaaaagcta
     7261 ttcagcaaga gctgggggaa ttagacgaga gaagttcgga aataaaagaa ctgaaggaaa
     7321 aattggaaag tctggatcta cctcctgaag tacgaaagga ggcagagaag gaaatagaaa
     7381 gactctctcg tctttctcct aattcttttg aatatccggt aatcaggaat tacctggatt
     7441 ggattattga actgccctgg aaggttagta cagaggataa tttagacata gatttagcca
     7501 ggaaaatact ggatgaagac caccatgatt tagaaaaagt aaaggaaaga attttagaat
     7561 atttagcagt gtataagtta aaaaaagata ttaaaggacc aatactttgt tttgttggtc
     7621 ctccaggggt aggaaagacc tctttaggga aatctattgc ccgggctatg ggaagaaaat
     7681 ttgtgcggat ttctttggga ggcataaggg atgaggcaga aatccgcggt catcgtcgta
     7741 cttatgtagg agcgctgcca ggaaggataa ttcagggtat caggaaatca ggaactaata
     7801 atccggtctt catgctcgat gaagtagata aaatgggcat ggattttaga ggagaccctg
     7861 cttctgctct tttagaggtt ttagacccgg aacaaaattc taattttgta gaccattacc
     7921 tgggtgttcc ttttgacctt tctcgggtta tgtttatcgc cactgctaat gttctggata
     7981 ccattcccct tcctctttta gaccggatgg aagttatttt cctttccggt tataccgatt
     8041 tagaaaaaat agccattgct caacgatacc ttatacctaa gcagtgtgag caaaatgggc
     8101 ttcagcctca aaatgttatc atagaggcag atattattca gaagataatt agagaataca
     8161 cccgggaagc aggagtgcgt aatttagaga ggaatattgc tactattttc cggaaaatag
     8221 ctaaagaggt agcccaaggg gttcagggtc cttttgtagt ggatgaggag aaactgagag
     8281 agtttttagg ccctcgccgg tttttggatg aagtgatgga agggaaagat gaaataggag
     8341 tagctacggg tttagcctgg actgaaagcg gaggagaaat actttttgta gaaacggtag
     8401 ttttaccagg taagggaagt cttcttttaa ctggaaatat gggagagatt atgcaggagt
     8461 cagctaaaat tgctctttct tgtgtccgag ctcgagcagg agagtggaac attcctcctg
     8521 atttttatga gaagaatgat atccatgtac atgttcctgc tggtgctatt cccaaagatg
     8581 gaccatcagc aggaattact atggtagtat ctatgatttc tgctctggcc caaatacctg
     8641 ttcgtcacga tgtggctatg actggagaaa ttactcttac tggtaaagta ttacctattg
     8701 gcggagtaaa agaaaaggtt ttagctgctt atcgggcagg aattagaaaa gtaattcttc
     8761 ctcgagaaaa cgagaaagat ttacaggaag taccggaaga agttaaaaaa acaatgaatt
     8821 tctccctggt agagaatata aatcaagttc tggatttagc tctggttaaa gaggaattag
     8881 tggaggttaa aaattaagga ggtaaaacaa taatggaaaa agaccggata gattttctta
     8941 ggaatcttat tgatactccc agtccctctg gttttgaaga aaaggtgcag ggagtttttt
     9001 tagaacgaat taaagatcga gtagaccaat attacaaaga cgttcacggt aatgctttcg
     9061 gggtagttaa tccagaaggg aaaattaaag ttatgctggc tggtcactgt gacgagatag
     9121 gaatgatggt agaatatgta aatgaccagg gatatattta cttttccacg gtagggggga
     9181 ttgattctca tattaccccg ggaaaacaag tgctcattca tacatcagga ggagtaatac
     9241 caggagtgat aggtaaaaaa cctatccatc ttctggaaga aaaagaacga caaaaggtgg
     9301 caaaaattga ggaacagtgg atagatattg gtgcccagag caaggaagag gctttaaagc
     9361 aagttactgt gggtgaccct attacttttg atgcaggatt taagtctctt ttaggcacta
     9421 gagtagtggg aaaaggattt gatgataaag tgggagcatt tatagtaggg gaagtattac
     9481 gctgtctcga aaaagataag ttacattgtg ctgtttatgg agtgagtaca gtgcaagaag
     9541 aattagggtt gcgaggagcc aggaccagtg cttttggtat agctccggaa ataggcattg
     9601 ctattgatgt tacttttgcc tctgattgtc cggatataga taaaaggaag gtgggagaag
     9661 tagcattagg taaaggcccg gttatttctc gaggtcctaa tataaatcct cgtatctggc
     9721 agcggatgat aaatatagct aaagaggggg atatccctta tcaagtgcag gccgaatccc
     9781 gtgctacagg tactgatgct aatgctattc agattactcg agaaggagtg gctacaggat
     9841 taattggtat tcctaaccgt tacatgcaca ctccggggga agtagtggat ttagaggatc
     9901 tagaacatgc tattgaactt ttagtacgat tcttacacag tttagaggga aatgaggaat
     9961 ggattccttg agaggagaac agttactctt aaatgccaga gtttagaaaa gatccgctat
    10021 ttgggagatg ggttattatt gccgaagaaa gaggcactaa accttatcaa tttggtgaag
    10081 gctgggagag agaaagttcg tttccctgtg tcttttgccc gggtagggag tcttatactc
    10141 ctccagaggt attcagtatc cgggaaaagg atactcctcc caacgagaga ggctggagag
    10201 tgagagtggt gcccaatagg tttcctgctc ttttacctgg tgagcctttg cgtcagtatg
    10261 tcgatggatt ttatgaaaga atgagtggtt ttggggtgca cgaagtaata atagaaactc
    10321 ccgaccacta ttcagacctg gttgaccttc ccctgaaaca ggtagaagag gtgctctggg
    10381 tttatcgaga gagaatgaga gaattaggag aagactctcg cttacgttat tgtctaattt
    10441 ttaaaaatca aggcgagaaa gcaggagctt ctctacctca tgctcattct caattaattg
    10501 ctactccggt tattcctaaa aaagtacagg aggaattaaa aggggcgaga gaattttatg
    10561 aggaagaggg aaattgtttt ttttgttccc ttctggaaga ggagagaaaa agagaacgat
    10621 tggtgtggga ggaagagaga tttatagcct ttgtccctta tgctgctcgt tttccttatg
    10681 aaacctggat tttccctcag cgccactgtg cttttttctc agaaatagaa gaagaagaaa
    10741 taagaaatct tgcttacgtt ctgaaaggag ttctttcggc aattaaaact cttcttcatg
    10801 acccggcttt caattttatc ttacatgcgg caccttattc ccgctctttg gagaactatg
    10861 cagattatta ccattggcat atggagatat tgccttcttt aggtaaaatg gcggggtttg
    10921 aatggggtac aggattttat attaattcgg taattccaga aaaagcggcg aaagctcttc
    10981 gccaatttat aaaagataaa aattaggtga ggaatttgtc tatcaaatat gctatgattt
    11041 tagcaggtgg aaaaggagac cgtttgagcg ttctttctgt agacagagcg aaagcggctg
    11101 ttccttttgg aggatgttat cgtcttatcg attttcctct cagtaattgt gtaaattcgg
    11161 gcatttatga tgttggtata cttactcagt atcagccccg atctcttatt gagcatatcg
    11221 gggtaggacg tccctgggat ttggaccgga agaagggggg gatagaattt ttacaaccct
    11281 acttgagtag aacagctaaa ggctggtatc gaggaacagc agatgctatt tatcaaaacc
    11341 tcaatatcct gagccgtaag aagatagctc atcttttagt tctttctggt gaccatgttt
    11401 atctgatgga ctataatcct ttgttttctt atcacttaga aagagatgct gatgtaacgc
    11461 tggtagctac tcgagtagat aaaaaagatg ctcctcgttt tggtatcatg gaagtggacg
    11521 aaaacaactg gattaccgga tttgaagaaa agcctttttc tcctcgtact gatattgctt
    11581 ttatgggtat atacatcttt cggatggaat ttttggtaga aaaattgata aataatgctc
    11641 aggaaggaaa gtttgatctg gttcgtaatg ttataatcga aaatgtgggg aaagctaaaa
    11701 tgcaagctta tttctataat gattgctggt ttgatgtagg aacggttaaa gcatactggg
    11761 aagctaacat gcaccttttg gaacctctac ctgcttttaa tctttacaat cccgattgga
    11821 tggtttttac taaccggggg cattatcctc ctactcatac tttagaaaaa gcagatatat
    11881 catccagtat aataggagaa ggggcagtaa ttaaaggtaa agtagttcat tccgtaattt
    11941 tccccggagt ggtattggaa gaaggaacag aagtgataga ctctattgtt ttcaataata
    12001 cggtagtaaa ggaaggagct aaagtaaatt taagcatcct ggataaaaat atagtagtag
    12061 gacaggacgt cattattggt tacggagaag attttactcc caacaaagcc cgtcctgagc
    12121 ttttagactg gggggttaat gtggtgggga aggggagtat aattcctgct cgtatggtta
    12181 tacatcgtaa ctgtcttata ggtataggga taaaagaaga acattttggt gagagaaaag
    12241 agcttaagag tggcgaagtc ctgctttgaa agaaatggga ggtcgagaaa ttgcttctgg
    12301 gtattttatt aattgtagtg gttctttatg ggcttttact cttctggata cttaatgaaa
    12361 gactgaagga tctggattac tcggttaata aagtgcaaga aagcttgaaa gagatggata
    12421 ataagttagt tcttttattg aacttaaaaa gtgaggaaga agaaaaagat agcgctctgg
    12481 aagaaacttt ttcccttcat attttacagg gagaaactaa aaagaaagga gataagtctt
    12541 aatgaaaaag caagcaattt tctggtcagt ggttttaatt ctacttttga gtttaggatg
    12601 gggagtattc gctcaagaag ttcaagaaac tcaacaggaa gttcaagaaa ttcaggagga
    12661 aagtagcaaa aaaactgtgg tagctgaggt aaatggagaa cctatttatc ttgaggatgt
    12721 acaggagatt tgggataccc ttccggaagt ctatcgaatc caatttccag gagggattca
    12781 agacatttta gagcagttaa ttagggaaac tttgctggtt cagcaagctc aaaaattagg
    12841 tttagaaaat gaccctgtaa tactggagca attggaacag attaagcggc agcttttagt
    12901 aacggagttg attaaaagag aaatagtgga taaagttcag gtaagcgacg aagaaattga
    12961 aaaggagtat acttccaatc cttctcttta tactgagccg gagcaggtaa aagctaaaca
    13021 cattatgacc tctactaaag aaaaagctga cgaggttttg caggaattac aatctggtag
    13081 gccatttgaa gaagtagcga aggaaaaatc ggaatctccc gatgcccagc aagggggaga
    13141 gatgggttac ctgaaaaaag gagatttaga ccctaattta gaaaaagttc tttttgacct
    13201 tgctccaggt aaatttagtg atgttgtaga aaccagctat ggatttcaca ttttcttggt
    13261 agaagaacac cttaaacccc gtcttaagga agtagaagaa gtaaaagatg aaatatctaa
    13321 ccgccttctt cctcggaaac aacaagaggc ctttgagaaa ttaattgagg aactaaaaag
    13381 tcaaacagaa atcgcaataa tagaagaaaa tctccctaaa gaggcaactc cgcaaccaga
    13441 agcctctcca gagacaggtg gggaaagcca gccatgatgg aagttctgga gaagttgatt
    13501 cgtcttcccc agaagggaga gtacatggaa gttcgtctgg aaagtatttt ttccaccacc
    13561 atacgggttc aaaatggggt agtagaaaag ataagagtgg ggacggatga gggaggctac
    13621 atccgtctcc ttgctcctcg aggtagttgg tggtttgaaa gttttcaaca ctgggatggg
    13681 ttgcaagaga aagtagaaag agctataaat ggcgcctctc ttttacctaa aagcgacatt
    13741 gaagtaataa gaaaaccacc agttgaggat gaaaaaaaag tcactctcac tgatgatttt
    13801 cgttttaaat cccttaaaga taagacctct cttcttctct cgtatgaccg aattttaagg
    13861 gaaatttctc cactgctggt aagttcttta gtggaatatc gggatgaatt ccgacagatt
    13921 tatttagcta attctgatgg ttctttgatt tattgggaaa aacctgatat cagcataagt
    13981 tttatggcta cggcaagaaa aggagataac atagaagtat atagaaatgg agtggcgggg
    14041 aaagcagggt ttgaactggt taaaaatcaa gaaactttag ctcaagaagt agggggaaaa
    14101 gcccttcttc ttttagaagc tccttctttc tctggaggag tatatgacgt aattttggac
    14161 ccggtactag cgggagtttt tattcatgaa gcatttgggc atcttagtga atctgacact
    14221 ctttaccaca atgaagaact gaaaaaaata atggctaaag gtaaaaaagt atcttcgcca
    14281 atattaaacg ttttcgatga cggaagttta gataatttgc gagggtcttc tccctacgat
    14341 gatgaaggta tacctacagg tagaacttat cttattaaag atggttattt gagcggaaga
    14401 ctccattccc gggaaactgc ttttttaatg caagaagaaa caacaggtaa tgcccggact
    14461 attaactatg gttttcctcc tatagtgcgg atgactaata cagctataga ggaaggtaaa
    14521 accactcgag aagatttgtt ttctgacata gaacggggat tgtatgctgt agaatatatt
    14581 ggtggaaata ccgctcttga gctatttact ttttccgctg cttatggcta cttgattgaa
    14641 aagggtaaaa taaaagaacc ggttaaagat gtggtactga gtggaaatct attccagact
    14701 ctggataaaa tagatgcagt ggctaacgat tttcgctgga cagaagtggg gggatgcgga
    14761 aaaggggggc agatgggact tcctactcct acgggaagtc cacacatacg actgagagaa
    14821 gttttagtgg gaggtagtca taatgattga agtagcgagc ttgcttaaaa aaagagggat
    14881 acctggtgat atatttgctc accagatttt taaccaggag gtgagctatg aggctaatct
    14941 ttttaagggg tacaggaaag aagaaagaga aggttatggt ctgcgagtaa ttaatccgga
    15001 ggggaaatta ggattttatg ctttcaatcg tcttttaaat cctcaagaag ctctggataa
    15061 agctttagag gttagtcagt taggagatcg ggtggatttt tctattccca cttatccatc
    15121 ttcctgggaa tgggaaaatt ttgttgaccc ccgggtagag aatttggaag cacaggagat
    15181 gatagagctg ggagattaca ttctttcccg gataagggaa gaatatcccc gggttttggt
    15241 ggatatagtt atcagtgcgg gtaaggaaga aaaatctctc ttgaatcacc atgaagaaga
    15301 gctaaaattt gctcgaacct tttttgaaat aggagtaagc gctaatcaaa ccagagaagg
    15361 agatatttta gaagtttata ccgaaaggag ctggggtaac cgagacatcg atgtagaagg
    15421 tctggtacag gagatagttt ccaaactgga gttttctcag agggtagcta atttaaatag
    15481 cggaaaatac ccggtagtct ttactccttc tggtagtctg gttttattta ttgccctctt
    15541 ttatggattg aatggtaaaa actgggtatt tgggaggtcg cctctggggg acaaagaagg
    15601 gaaagtgcta ttttcctctc ttttttcctt agaagaaata caagagccct gggctttggg
    15661 atcctgtcct tttgatgatg agggaataat gactaaagct caaaggtcta tcattgatag
    15721 aggaagggtg gaaagtgctt tctgggattt gtggagtgca gcaaagaagg ggattaaacc
    15781 tacaggaaat ggttttcgca gctcatatcg agatttaccc gagccaggat tttcttctct
    15841 ccgggttaag aatggtttac gggataggga tactttaata ggagagctgg aagaaggttt
    15901 aatagtagat agtgtcctgg gcttagggca gagtaacgtt gcttctggct atttttcctg
    15961 taatgtccag ctaggtttct atgttaaaaa aggagaaatc cagggacggg taaaagatgt
    16021 tatgattagt ggaaatgctt atccggctct ttttaatctt aaagaaattt ctcgggatag
    16081 tcgctggata gggggcagga ttttgcttcc ttaccttttg atagagccag tagaagtcaa
    16141 taagtaatca tcaagaggca agagtctatt aataaaagca ggaaggtgag aattgatgtt
    16201 caaagctctg ggcaagcttt cagatttagc ccaacaagaa gaagagattt taaaattctg
    16261 gaaagaaaaa gatatattca aaaagagcct gaaacagaga gaaggctcat cccgctttac
    16321 cttttacgaa ggtcctccta ctactaatgg ctatcctcat gccggtcata tcattggccg
    16381 cagcattaaa gatttgattc ctcgttatcg gactatgtgt ggttattatg ttcctcgccg
    16441 ggcaggctgg gatacccatg gtcttccggt agagctggaa gtagaaaaag aattgggaat
    16501 ttcgggaaaa ccagatatcg aacgctatgg agtagataaa tttaacgaga agtgtaaaga
    16561 aagtgttttt aagtacatta aagaatggga aaaactaact gagagattag gtatctggat
    16621 ggatatggaa cacccttata ttacctgtag taatgactac attgagactt tgtggtgggt
    16681 gttaaaagaa ctatgggaga ggaagctgct gtataagggg cataaagttt taccctattg
    16741 cgctcgttgc ggaacctctc tttctagcca tgaggtggcg ctgggttatc gggatacgga
    16801 agacccctcg gtttatgtta aatttgcttt acagaacagg gaaaaaactt attttctggt
    16861 gtggactacc actccctgga ctttggtggc caacgtggct ttggcagtgg gtaagggtat
    16921 tgattatttg gaagtggtca atgaggaaaa tggtgaacat tatatccttg gtgaagatag
    16981 tttttatcgt ttgctgggag aagatgctaa ttataagata gtcagaaaat ataaaggaga
    17041 ggaattgacg gggataaaat acgaaccctt actggatttt ctggtggcag ataaaggata
    17101 cgaagttttc tctgccgatt ttgtcactac tgaagaaggg actggggtag tacatattgc
    17161 ccctgctttt ggtgaagacg atctgcagct agctcaaaga gaaggacttc ctcttttgca
    17221 gccggtaaag cgagatggaa aatatggaga agaaattact ccctggcagg gaatgtgggt
    17281 aaaagatgcc gatcctttga ttatcgacta tttaaaagaa aaagggaagt tatttaagca
    17341 agaaatttat aggcattctt atcctttctg ctggaggtgt gatactcctc tcctttacta
    17401 tgctaaagaa agctggttta ttaaaaccac cgcttttcga gattcactac tggaaaataa
    17461 ccaaaaaatt aactggtatc ctcctcatat taaagaggga aggtttgggg acttcttgtc
    17521 caatgtagta gattgggctc tttctcgaga gagatattgg ggaacgcctc tcaatatctg
    17581 gcagtgcgaa agttgtgatt ttcaagaagc tgttggttct atagaagagt taaagagcag
    17641 atcacgagaa aagatagaag aaatcgaatt acatcgccct tacgtggacg gagtcactct
    17701 ttcttgcccc cgctgcggtg gagcgatgaa aagagtgcca gaagtgatcg attgctggtt
    17761 tgattctgga gcaatgtttg tagctcaact tcattttcct tttgagaata aagaagaatt
    17821 tcaacaaagt tttcccgctg actttgtctg tgaggctata gatcagaccc gaggctggtt
    17881 ttacagtctc catgttttag ccaccgttct ttttaatagc ccagcgtatc gcaattgcct
    17941 ggttactgaa ttagggctgg atgaaaaagg gcagaagatg agtaaacaca taggcaacgt
    18001 cgtcaatccc tgggagctaa ttgaaaacta tggagctgat gtattacgct ggtacgtttt
    18061 ttctgtttct cctccctggg tacctaaaag attcgggaaa gaagcaataa gaggagttct
    18121 tagcaaattt tttgatactc tgtggaatgt aactaacttt tttattcttt atgctaatat
    18181 tgacgctttt gagccgccaa tttctttttt gcccctggaa aaaaggagtg ctttcgaccg
    18241 gtggattata tcttgcctgg aagaacttat aaaagaagtg cgaagttcat tagatcacta
    18301 tgaggtctct cgagcgagta aagctataga agattttgta atagaagatt taagcaactg
    18361 gtatatcaga cgctctcgtc gccgcttctg gaaagcagag aaagacgaag ataaaattag
    18421 tgcctatcat acactttatt atgttcttac aaacctgacc cggcttttag ctccttttat
    18481 tcctgctacc gccgaactgc tttatcaaat tctggaagct gatgttcagg gaaaaaaaga
    18541 aagcgttcat ctggaaaatt atcctttacc cgaggaaaaa aatatagata gagagctgat
    18601 acttgctatg gaggtggtaa gaaaacttac caacttaggc cgttcggttc gcaataaagt
    18661 gaatatcaaa ttgagacaac ctctcaggaa agcaatttta gtagttcccc aagcgagaga
    18721 aagagatcga gctcttcctt ttgcttctat tattcaggaa gagttaaatg ttagagaaat
    18781 agaatggatt tctcatcttc ctgaagaagt cactgtttct ttgaagcctc gtttttccat
    18841 tttgggccct cgatatggcc aaaaggtgaa agaaatagct cggttgttga aaaatctgga
    18901 agaagataag agccgccaat tctgggaaga gggagaagta tctttacttt tagagggaga
    18961 agagattata ctaaagcgag aagaagtaga attagaattg caaacccggg aaggccttgc
    19021 agtagaaagt gaaggagagt acgcggttat tttagatact ttcttagatc aagggcttca
    19081 ggaggaagga atggtaagag atttaatcca tgccatccag gtttggcgta aagaagctgg
    19141 cttagaagta gaagaccgga tcgaattaca agttagagaa ggtactgacc cagaatggag
    19201 agatttgata gaaaaatata gttccttgat tcaaagtgag gttttagctt cctctctttc
    19261 tttatccagt aatggaggag gagaaatgac cagcagagaa tgggagctaa gcgggaaaaa
    19321 attagcattg gctttaagga ggaaatgata atggaatcaa agaggagact tcttttcctg
    19381 tttctatttt tagtcagtac ctgggttttt cctgcctggg cagatagtta tcatgtggta
    19441 gaatcaggag atactctttg ggccatatct cgccgttatc agatacctct cgaaaaactt
    19501 ttgcagatta atggtcttac ggaaaaatct accctccgga taggacagaa aatcaaatta
    19561 gaagagggag ctaacgaggc aatctaccag attaaagcgg gagatactct ctggggaata
    19621 tcccgtcaat ttaacattcc tctttcttct cttcttgcag ctaatggtct tacggaaaaa
    19681 tctatcctcc gggtgggaca gaaaattatt ttaccttctt cctcaaaaga agaagccaag
    19741 gctgtcgtta cctcttctaa tgttactcat atagttaaaa agggagaatc cttatggttg
    19801 atatctcgcc tttatggggt ggatatggat tccattatga aagctaataa attaactgct
    19861 gatagtattc tctccattgg catgaaactc accattcccc aggttcgtgt tgcttcttct
    19921 ccggtaaaag aagataactt tgaagtttat cgggtccaat ccggagatac cctttgggat
    19981 atatcccgcc gttatcgggt ttccttacag gatttgatga aagttaataa ccttaatgag
    20041 aaaagtactt tgcaaatagg gcaaagtatt cgtatacctt cttcccttcc taaaaattat
    20101 gaaaaagcca gcagttcatt cctctggcct gtttctggcc gggtttcttc tacttttggt
    20161 atgcgaggag gaaggcttca ctccggggtt gacgttttag ctccttcggg tacgctgatc
    20221 aaagcttctc gtagtggagt ggtttctttt agtggatgga tgaatggcta tggtagagtg
    20281 gtgatagttg accatcaaga tggctggcaa actttatatg ctcataattc cgttaatcta
    20341 gttaataaag gacaaaaagt aaaccaggga gatgccattg ctcgagtggg gatgacaggg
    20401 aatgctacta cttgccatgt tcactttgaa gtgagaaaaa atggtaagtg tcttgaccct
    20461 ttaaactatt taggtaagtg aaatggaaag agaagaagcc tggactttat taaaaaaaca
    20521 tctgaaaaat cgaaatcttt taaaacacag tatagcctgt gaggttatta tgcagttttt
    20581 agctcgtcaa tggggagaaa atgaaaaaga atgggctcta gctggacttc tgcatgatat
    20641 agattatgaa gaaaccaatg gcgaacttac ggagcacgga agactgggag ccgaaatact
    20701 caaagaagca ggtcttcctg aagaaataat cagaacagtt caggctcaca atcccgaagc
    20761 tactggtatt aagcctgaat ctcttatgga aaaagccctt tatgctgtag acccggtaag
    20821 cggttttatt gtggcctgtg ctctcataca tccagaaaaa aagctggata gtttagacct
    20881 ttcttttctt cttcatcgtt tttcagagaa aagttttgct cgaggtgcca atagagaagc
    20941 gatgaaaagc atagaaagta caggcctctc tctggaagaa tttttgaaaa tcgctctctc
    21001 agctatgcaa accaaaaaag aagagctggg tttataactt ttgaggtttg tttgaccatt
    21061 cttgacaaaa ttatgagctt cttttataat tactccgtaa ggtcaagaaa ggtcaaagga
    21121 ggtaaagtaa gatggctatt aatcgttggg atccttttcg agacttagct gagttacagg
    21181 gagaaattga tcgtgtttta gggagcactt tctctcgtag aggaaaagaa actactgtgg
    21241 gaagctggct tcctcctgtg gatgtgaaag aagaggagga ggagtttgtg ctgtttgttg
    21301 atctacctgg ggtagacgaa aaggcagtgg aaattactgt ggttggagat cagttaacca
    21361 tcaaaggaga aagaaaagca ccagaagagg gagagaactt cttgcgtcag gagcgggttt
    21421 ttggtccatt ccatcgttct tttctcctta ggatgcctgt agaagtggac aaagtgtcag
    21481 ccacctataa aaatggagtt ttagaaattc atttgcccaa atcttctgct gctaaaccca
    21541 ggaaggtaag cgttcaggta gagtaaaaga ggtagaataa ttccgggggt ggaaattttc
    21601 cacccccaga ggtgataaaa attggaattc aaagattact atcaaatttt gggggttccc
    21661 cgaggagcta gcgaaaaaga gattaaaaaa gcctatcggc gtttagcccg tcaataccat
    21721 cctgacctta atccaggcaa taaagaagca gaaaaaaaat ttaaagaggt taatgaagcc
    21781 tatgaggtat tgagtgaccc agaaaaaagg aaaaggtatg atgaattagg gtcggcatgg
    21841 aataattatg gaagacagaa tactgacgaa ttctggagtg atttttatag aagatatgga
    21901 ggagggccgg cttataccac ccaagaggaa gaatttgaag caggagattt tagtgatttc
    21961 tttaaaacta tctttggtga tttattagga ggaactcgtc agtcctctcg aggacggggt
    22021 tttcgtttcc atactcaggg tgagggaata agaagagagc cgcctcctga agtacaaagc
    22081 gaggtggaaa tttcttttga agaaagtttt cgaggaacag atcgaaatat ccaggtagag
    22141 tatgaagagt tatgctctaa ttgtggagga acgggtagaa atagtgaggg gaaactgtgt
    22201 cggagttgtg gaggaagggg aaaagaaaag aaatctcgaa aggtaaacgt tgccatccct
    22261 cccggagtta gtgatggtac aaaattgaga attccccaga tattgggagg gagagactta
    22321 tatttgaaag ttcgggtaaa accccatccc tttttccgta gggaagggga taatgtcatt
    22381 ttagaactgc cagttactat ttatgaagca actttaggaa cagaggttga agtccccact
    22441 ttagagggta aagccaagat gaaaattcct cctgaaactc aaaatggaac tatttttcgt
    22501 ctgcggggtt taggttttcc tcggactaaa gaaggaggaa gaggagacca attagtgaaa
    22561 atcaaagtgg tagtgccccg ggggttaact gaaagggaaa gaaaattatt ggaggaatta
    22621 gcttctttac ggaaagagaa tcctcgccaa catctggtga gaatttgaga ggtaatggta
    22681 tggaaaaaga aaagaaacct aatggatatt ttcttattaa agaggtggca aacataactc
    22741 agttgcaccc tcaaactctg cgttactacg aaaagatagg gctggtgaaa cctcatcgca
    22801 gccagggtaa tgctcggctt tatagtttag aagatataga acgaattaaa aaaataaaag
    22861 tttacactcg agatttagga gttaacttag ccggtgtaga agtgattttt aagcttactg
    22921 aacaaataga ggagttacaa aaagaaaagg ataaaataaa agaacagcta gaagtaaaaa
    22981 ataataaaat ggatactgaa cctccagtta aagagcgaag gagaaaaata aaatgaggtt
    23041 tgagcaattt actgaaaaag ctaaagaagt tatctcttta gctcaagata ttttattgga
    23101 ataccatcat aaccagctgg atgtagagca tctcctttta gctcttttga agcaaaaaga
    23161 gggattggct ggccagattc tggagaaatg taaagttgat ccccagattg tagctcgaga
    23221 agtcgaagaa gctctgcaac gttttcctca ggtttattac caggggggcg aggagatgca
    23281 gatttatctt acccctcgag ctaaatcagt tttagataat gctgaagaag aagcacagag
    23341 gctaaaagat tcctatgtag gggtagaaca tctcctgata gcggtggttc gggaagaaag
    23401 cggagcaagt agtcgtattc ttaaatctca cgggttggat gtagaaaaaa tttaccaggc
    23461 tttgcaggaa ataaggggaa aaagaagggt agatgatcct tatgctgaag ggaagtacat
    23521 ggctttagaa aaatatggta gagacttaac ccagctggct cgggaaggta aattagaccc
    23581 ggttattggt agagatgagg aaattaaaag agtaattcag attttgagcc gtcgtactaa
    23641 aaataacccg gttttaattg gtgacccagg agtgggtaag accgctattg tagaagggtt
    23701 ggctcagaaa attgttaagg gagacgttcc cgaaagtttg aaaggtaaaa gattaattgc
    23761 tctggatatg ggagcactgt tagctggtgc gaaatttaga ggagaatttg aagagagatt
    23821 gaaggcagtt cttgaggaag tacagaagtc agaagggaaa atcattcttt tcatcgatga
    23881 acttcacact gtagtggggg caggagcagc agaaggagca attgatgccg ctaatatttt
    23941 aaagcctgct ctggctcgag gagaattgcg ctgtattgga gctacaactc tggatgagta
    24001 tcgaaaatac attgaaaaag attcagctct ggagcgtcgc ttccagccag tgtatatagg
    24061 agagccatct atagaagaaa ctaaagaaat acttaaaggt ctgcgagata aatatgaagc
    24121 tcatcatcgg gtaaaaatca gtgacaatgc tattgaggcc gcttctgaac tatctagcaa
    24181 atatattact gaccgttttc tacctgacaa agctatagat ttgatggatg aagcagccag
    24241 taaaaagaga atagaaatag agagtgtccc ttcagagata aaaactttgg aagaagaatt
    24301 gcaaaaatta gctaaagaag gtatggcggc agtgcaagct caggagtatg aaaaagctgc
    24361 tgccttacga gataaagcag aagcattgca aaagaaatac caggaagaga aggaaaaatg
    24421 gatgaaagag cgggagatta gcgaagtggt gggggaagaa gacattgctc agatagtatc
    24481 cagctggaca gggataccag tagctaaatt ggtagaaaaa gagaaagata ggttgcttca
    24541 tatggaagaa gagttgcata aaagagtagt gggacaagat gatgcagtca gggcaatagc
    24601 agaagctata cgtcgttctc gagctggtat tactgaagga agaagaccaa taggttcttt
    24661 tctctttctt ggtcctactg gggtaggtaa aacggagtta gctaaatctt tagctgattt
    24721 tctatttggt gatgaaaatg ccattttgcg tattgacatg tctgaatata tggaaaagca
    24781 ttctgtctct cgtctgattg gtgcccctcc cggatatgtg ggctatgaag agggagggca
    24841 attaaccgag gtagtaagaa gaagacctta tcaagttatt ctctttgatg aaatagagaa
    24901 ggcccatcct gatgttttta atattttgtt gcaaatactg gatgatggaa ggctgactga
    24961 tggccaagga agaacagtag actttacgaa cacggttatc attatgactt ctaacttggg
    25021 aagctcttac tttcgaggaa tagatccgga aaaagataga gaaagtctgc gtcgagctaa
    25081 agagaaagtt ttggaagagg tacgaaatca ttttaaaccg gaatttttaa atagaattga
    25141 tgaaataatt gtatttaacc ctctgagtaa agaggaaata aagaaaatcg tggatttgat
    25201 gataaatcgt ttacgaaatc gacttcaagt tcaaggaatg gatatagtcc ttactcaaga
    25261 ggtaaaaaat tatttagctc aaacaggtta tgaccctgat tacggagctc gtcctcttcg
    25321 ccgtctaatt caacgggaga tagaaagtcc tctttctggg gaactgattc ggggcacttt
    25381 tcagcctggt gacgttatag aagtttattt agaagaaggc gagataaaat tccggcgta
//
LOCUS       DAOSVE010000017        24140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_1367_length_24140_cov_68.588001, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000017 DAOSVE010000000
VERSION     DAOSVE010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 24140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24140
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1367_length_24140_cov_68.588001"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..137
                     /locus_tag="PK016_02640"
     tRNA            62..137
                     /locus_tag="PK016_02640"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:95..97,aa:Ala,seq:tgc)
     gene            complement(144..266)
                     /locus_tag="PK016_02645"
     CDS             complement(144..266)
                     /locus_tag="PK016_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02005.1"
                     /translation="MRKMIAAYCNLIPCPREKEKKQKQKEEKETEKIKVAEIGF"
     gene            931..1899
                     /locus_tag="PK016_02650"
     CDS             931..1899
                     /locus_tag="PK016_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL02006.1"
                     /translation="MQQETTEKKNSRNFHFREMNILVALILMCLLITIMNPRFLNVFN
                     LQTIARQVAIYGLLAVGETLVIITGGIDLSVGSLVALTGVLVALFMKSGLGVVFSIIV
                     VLFISSVIGLTHGLFVTKLNVPPFIITLGTLTAARGLASAITKGWPIIGLPESFFFIG
                     QGYIVGIPFPLIILLIFAAVAFFVTRYTVLGRQLYATGGNIEAARLSGVDVDRRRMFA
                     YIFNSFLAGVTGVILASRLSQGQAGVGSGYELNAIAAAVIGGASLAGGEGTILGALIG
                     ATIMSVIYDGLILLEISSYWHDVVMGLVIVAAVTIDVWRKRRSKTR"
     gene            1985..3004
                     /locus_tag="PK016_02655"
     CDS             1985..3004
                     /locus_tag="PK016_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HXL02007.1"
                     /translation="MRKFKLSLVFALCFLVLFVSAGYAEEIHVAVIGKSVHPYWAEVE
                     LGVKKAAEDLGVKATFFVPQKEDIQAQISQMESFIAMGVNGIAIAPSDPTALAPTIET
                     AISKGISVITLDTDAPESKRLAYIGTDNYSAGKLAGQVMAQLLPEGGEVAIGTGSLTA
                     MNSLERMRGFTDGIAENENIVVVTKPALCDFEDTGRAVTLVEQALLTYPNLKGFFGVY
                     AINGPAAAKAIKTAEKVGEVLVVCFDTTAEHMQLIKEGAIAATIGQRPYMMGYKSVEL
                     LSKMAAEGVDAALAELPENRIIDTGVDIVAADAYVETLRSLGANSVEEYREKLSGLGI
                     PVEGW"
     gene            3060..>4468
                     /locus_tag="PK016_02660"
     CDS             3060..>4468
                     /locus_tag="PK016_02660"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HXL02008.1"
                     /translation="MKQELLLKMEKISKAFPGVQALSGVDFEVYKGEILALVGENGAG
                     KSTLMKILTGVFQKDEGKIIFKGKEIEARNPHHARQLGISMIYQEFSLVPNLDIATNI
                     FLGNEPTKRFDIFDYDKARVEAERLLKMVGLEVSPDVLVGDLTVAQQQIVEVAKALAS
                     KSELIIMDEPTSALSKEDSERLFGIMRKLKEEGISIIFITHRLEEVFSVADRIVVLRD
                     GQRVGELSCEETRISEVIKLMVGRDVRVVPRVGVSAGNVVLEVRNLSRPPMIKDVSFS
                     LKKGEVLGFAGLIGAGRTEIMRALFGADLKASGEIFIEGQKVEIKSPNDAVRLGIGFV
                     PEDRKLQGLILIMAVYENISLPILPFMFSNGIIDFKEEYSLAESLVERLNIKTPTIWQ
                     IVNNLSGGNQQKVVLAKWLALKPRVLILDEPTRGIDVGSKAEIHHLIDELAKEGIGVI
                     LISSELPEILALSDRILVVS"
     assembly_gap    4469..4568
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4810..5604
                     /locus_tag="PK016_02665"
     CDS             4810..5604
                     /locus_tag="PK016_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013523874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HXL02009.1"
                     /translation="MSVNIQNVIDTIMPPTLGQENTVDKLEFGDPETIVQGIAVTFLA
                     TQEVIEKSIKLGLNLIITHEGIFYNHLNKIKAIESDPVYQCKRETIKSGKIALFRFHD
                     TAHRYKTDVIMTGLLRRLGWDSYEVKKQQTYSVLIIPEMTLREVMMHIKKSLSIEYVR
                     FIGDLTMKCRRIGILVGYRGTGEMTIPLFHQENLDLMVYGEGPEWETPEYVRDTIQQG
                     RQKSLLVLGHAESEMPGMEYVASILREKFPDIPVHHISQSPLFLVF"
     gene            5781..7514
                     /locus_tag="PK016_02670"
     CDS             5781..7514
                     /locus_tag="PK016_02670"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL02010.1"
                     /translation="MKRAVLACLLVLVLACGGVAFGQQVQIPREEALYVAGFQWGPPT
                     TDNPFTGSPMTFVSNPRQHIWIYEPLFTYDGLLTGDYVPILGESYQWRDDLTLEVKLN
                     PNAYFHDGEKVTADDVVYAYKFGQKYPVGYSSIWDWLEDVYKEDDQTVIFKMKADNPN
                     KLMVEDGLGAVYIIPEHIWAKVEEDAGNNLEEIRKFDNKNPVGSGPYRVYYESPETII
                     LERVDNYWGVVLHDGELPKPKYVVHPIFKSNDEGNLAFENGEVDVSQQFMPRIWEMWE
                     NKGLPVGTWYKEIPYHVPATMPSLWFNVNKHPLDLPELRRAIAYCIDYAKISELAMSN
                     YSPQVEASLIVPYGAQAKYFNKELVQQEGWEYNPEKAVEILENELGAKKGNDGIYVLP
                     DGTRLGPFKVECPYGWTDWNAALEIVAQSAKAIGIDVQTSFPDSPVAYDNRQTGNFDM
                     TMWTPSEPSPAQPWLNFQIVLYSGGVPEYGKIAYRNFGRYKNERVDEIIDLIPKTSDE
                     AELQKLYDELDSIYRKDVPVIPLMYRPWVFYEYNETYWKGFPNADNPYAPGMPCVGAG
                     MEILWHIEPVK"
     gene            7596..8606
                     /locus_tag="PK016_02675"
     CDS             7596..8606
                     /locus_tag="PK016_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019003674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL02011.1"
                     /translation="MRKFRRYILQKLFWYVLAFFIALFLNFLLPRLVPGDPISVLVSQ
                     MMSGAVSSEAQERVYQSFIQEFGLDKPFFVQFFIYVKNVFKGDLGTSFSLYPLSVNKI
                     MADALPWTLFLQFPAIIVGWIVGNFLGALAAYRKGNFDRILFPVSLFINSIPYYCLAI
                     ILLYIFGVYLRWFPIGGGYSRTLLPFWSWTFVVDVLHHYFLPFISIVLVTIGGQAIGM
                     REMSIYELNTDYVTYCKMLGIGDRKIQSYVFRNAMLPQITGLAISLGTMIGGSLITEI
                     VFGYPGIGTTLFNGIRRLDYPLIQGSTLIIALTVLVANFIIDMVYGLVDPRIKAAQTE
                     GS"
     gene            8608..9441
                     /locus_tag="PK016_02680"
     CDS             8608..9441
                     /locus_tag="PK016_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL02012.1"
                     /translation="METLKLLLRSPKFIVGLGIFAVLFLTAFIYPAVVPKNPFEMVGL
                     MYEPPSSRYLLGTDNFGRDTFVELIHGMKTSLIIGLFSGIIATAIGVTIGLFAGYKGG
                     NVDNILNSITNIFLVIPPFVILILITVSLSSRSLVVMGLVLGITSWPWTARAVRAQSM
                     SLRGREHVDIARLNGASVVEIIWREIMPYILSYIFMAFVLQVASGILNEAGISMLGLG
                     PSNVVSLGTMLSWALLFESVRSGAWWAFVPPAITIALITFSLYFMNTGMDEIFNPRLR
                     S"
     gene            9444..10463
                     /locus_tag="PK016_02685"
     CDS             9444..10463
                     /locus_tag="PK016_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL02013.1"
                     /translation="MEESRKIILEIKGLKAYYRTRSGERIRAVDDVSFGLMEGEILGI
                     AGESGCGKSTLAMAMSGLFLPPLTYESGTVFLDGENIIGKKEEELRKKILGRRYSYIP
                     QSAMNALNPTLRIKNFVIDLLKEHHPNMSKKEVLDLTTERLKSLSLPPWVIDLYPLEL
                     SGGMKQRVVIAVSTLMNPKVVVADEPTSALDVTSQKIVIKLIKDLFDEGIVKSVIFIT
                     HELPILRHVCDRIAVMYAGEFVEMGKTEDIIFNPLHPYSQALMQSILVPEVGIKGKKL
                     PNLPGSPPDLRKPIKGCRFADRCYLAIEDCRKETINMTEVGGREVRCIRVDYIKAKEE
                     VAPYV"
     gene            10456..11256
                     /locus_tag="PK016_02690"
     CDS             10456..11256
                     /locus_tag="PK016_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL02014.1"
                     /translation="MSNNDEIIRTENLTRVFGSGKKAIKAVDNVNLSIKRKEIISLVG
                     QSGSGKTTLAKMILRLLKPTTGKIIFEGQDVTNIEGKEKKAYWRKVQAIFQDPYASFN
                     IFSPVKKVLLDAFKLFDNKFTREEKWKMVCESLEAVNFRPDEVADKFPFELSGGQRQR
                     IMIARAHLIKPELLIADEPTSMIDANLRSGILELLLDLRNTEGTTIMFVTHDLGLAYY
                     VSERLLIMHEGKIVEEGSADKIISKPEHPYTKQLIADVPKLSHEWLLE"
     gene            11660..12994
                     /locus_tag="PK016_02695"
     CDS             11660..12994
                     /locus_tag="PK016_02695"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HXL02015.1"
                     /translation="MVAKLFPEDFVWGVATASYQIEGSPLADGAGPSIWHRFSHTPGT
                     ILNGDTGDIADDHYRRYREDVALMKELGLKGYRFSIAWSRIFPYGKGKINEKGLDFYN
                     RLVDELVSAGITPFVTLYHWDLPAALQDLGGWANRDIACWYADYADFMFQRLGDRVKN
                     WITLNEPWVAAFVGYYEGRHAPGMRDLYAAFLATHNQLRAHSKAVRAFREETRGGKIG
                     ITLSNSSRDPVSSSKEDKEAAQLAHEWANYPLFLHPIYKGDYPEKVRERAREFLPPHY
                     EKDMAEIKEPIDFVGINYYYGVLVQADPQSPLGGKAVERGLPKTEMGWEIYPEGFYKI
                     LKGVQNEYDPAEVFVTENGAAFEDKVEDGKVHDEKRINYLQEHFGQALKAIEDGVKLK
                     GYFVWSLLDNFEWALGYSKRFGIVYVDYETQKRIIKDSGYWYSKVIKSNSIE"
     gene            13122..14183
                     /locus_tag="PK016_02700"
     CDS             13122..14183
                     /locus_tag="PK016_02700"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXL02016.1"
                     /translation="MAKIKVGVIGAGNIGVVHALAYRDVPEAELWAIADIDSERLFEV
                     QKRLEIPKAYTNYEDLLGDPEIEAVSICLPNFLHHRAACAALESGKHVLLEKPMAMNA
                     KEAHEIVDCAKKNGKILMIGFSYRFRSEVITLKSWVEEGLLGEVYHAQSIILRRRGIP
                     AMGSWFTTKAKSGGGALIDIGVHYLDLGMWLMGYPEPVSVSGFTFDKIATRQPYIYVD
                     MWGEPVPGGPFDVDEYAISLVRFQNGAALWMESSWALNAQDEDRVQIFGDKAGAKLSS
                     KEGLTLFGELGSKIADVKPQIKSQTPGAIYREEVLHFLQCVKEGKEPMASGEHGLIVQ
                     KVIDAIYESEKQQQEIKIS"
     gene            14205..14978
                     /locus_tag="PK016_02705"
     CDS             14205..14978
                     /locus_tag="PK016_02705"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HXL02017.1"
                     /translation="MRLAVSSWSLRDHVNRDFPLQDFPRVIKERYGVEAVELCQMHFR
                     AQDSKYLDEIINGLKAAGSVVINMPIDTGNISQLDERKRNHDIKIIEGWMDVAAYVGS
                     PNIRVNTGSQNQGVVDLSITVDSYRKLAEYGEKIGVMVLLENHGGISADPHNIVRLFE
                     EVGHKNFGACPDFGNFSPEVRYQGLEMMAKYAVIAHAKTYDFDEETKMSEEDFVKCLN
                     ILKSAGFDGYLSVEFEGKKDQYSGVKRAIELIKVSCKTD"
     gene            complement(15414..17306)
                     /locus_tag="PK016_02710"
     CDS             complement(15414..17306)
                     /locus_tag="PK016_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL02018.1"
                     /translation="MKKSSLILFVSLVILAMGFTQTGFAQLAEDIPREETLIATGPGP
                     RVATAGNFNVFTTTWRYVQCGIHQLMLEPLWMNEHVLGEIINALATEGPIYNEDFTQM
                     TVKLREGCYWSDGVPITADDIVYGIETTMKYPGMNYNAQFNLYVEKVYKTDDYTVVFE
                     LKEPNARFHTNFVDRWGAWRPFPKHIFEKVEDPVSFEFNPPVSSGPYILKSYDPAGYW
                     VLWERREDWDKTPTGILYGMPKPKYVLFPSYGEVTPIVIDQVNHNLDIADLDIEGFRA
                     VISKNPYSRGYRKEYPWVVNTDPCITGITFNTEAYPFNLKDVRWALTLAIDIVDYIAI
                     ALDGAATMGVLHVPPTPVYQKWYYEPMEEWLKNFTLDIEVNGERFKPYDPDAPLRAVE
                     YAKERGYSVSEDPEQIKEIFGPGWWKYAPDVAEQLLLRNGFTRDENGKWLLPDGTPWK
                     IVILAHPNPVHGQHRNSLAATQQWRNFGIDVEMRTSEAQGTLAGVGDFEVTDLWPATE
                     PWGGHPDLHRSFTPFYSSFYRPIGENTPGHVSRWTDSRLDEIVEEMEKVDWNDPRNME
                     LGMEGLKILIEEMPTIPTFGYPGVVGWDEYYWTNYPGAENPYSQPYHHFPNFKYMLPF
                     LEPVKR"
     gene            17840..18799
                     /gene="rbsK"
                     /locus_tag="PK016_02715"
     CDS             17840..18799
                     /gene="rbsK"
                     /locus_tag="PK016_02715"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HXL02019.1"
                     /translation="MSKPKILVVGSLNIDMILKIERMPEVGENLFVKEFSMLPGGKGN
                     NQAVACARLGALVSVIGKVGDDEFGERLFANLEQENVDFAFVTRQALTHTGLAFIFVD
                     EKGENRILVAPGANMELSITDLDNALELFKANEFLVLQLEIPQEVVKKCIEYAHRFGM
                     RVVLNPAPAQPFPIEILTANDIITPNKYEAEVLSGIKIKSLSDALKAIRVLDERCPAR
                     KVITLGGEGAVASYENNQFVYLPAREVKVEDTTAAGDAFNGGLVVGIGMGKDWIGALK
                     LANNAGAIACTKVGAQSALPHLRDVERLVKKIGEGEIKIILGD"
     gene            18844..20121
                     /gene="hisS"
                     /locus_tag="PK016_02720"
     CDS             18844..20121
                     /gene="hisS"
                     /locus_tag="PK016_02720"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HXL02020.1"
                     /translation="MSIIKAPKGTRDIWGEEIYLWHRVEEVVRSLASSFQYEEIRTPI
                     FESTDLFARGVGEVTDIVQKEMYTFPDKGGRSLTLRPEGTAPVMRAYLEHGLNISNPR
                     VKWFYMGPMFRYERPQAGRMRQFHQFGFEAIGFPQPACDAEIILIAWLVSKKLGLENL
                     SLEINSLGCNNCRDKYIKVLQDFLNREENNLCANCKTRTEKNPLRVLDCKEETCQEVI
                     HSSVFPVVQDFLCEECREHQENLMEILKELGIPFSINPYLVRGLDYYTRTAFEIKTGE
                     LGAQDAVAGGGRYDNLSSSLGGPPVPGVGFAAGMERMILLLAKKYEPSSFKEEPLFYL
                     APLDAPGEKTLLQLASFLGERAIPFYLEFADKGVKYHLKKAQKMGINNVIIVGSKERD
                     REEFIWKDMRVGEQRAMKREELLATLLEEREKC"
     gene            20115..21866
                     /gene="aspS"
                     /locus_tag="PK016_02725"
     CDS             20115..21866
                     /gene="aspS"
                     /locus_tag="PK016_02725"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HXL02021.1"
                     /translation="MLRTHTCGELRKEDAGKEVQLTGWVSRLRDHGGILFIDLRDRWG
                     ITQLVVDNKEEIYNRASRIRPEYVVKVKGVVKERPEGLVNPRISTGEVEVAVEDLEVL
                     STSELPPFEIEGNKETDESIRLRYRYLDLRRDKMQKNLILRHRVAQLIRRFLSEREFL
                     EVETPFLTKSTPEGARDFLVPSRLNPGHFYALPQSPQLFKQILMISGLDRYFQIVKCF
                     RDEDLRADRQPEFTQVDIEMSFIEPEDIIQLIEGMMGYVFKESLGVAIETPFPRLSYD
                     EAMEKYGTDKPDLRIPWEIDDLTELLGREITSFSRGGNNFAVKGLFFPSGEEFSRKKL
                     DVLSQKAKESSLSLSWVKGGSSALSSPLRGKLSEESWQAIEEKYPFGENTLLLLAWGE
                     KTDLVNFMGGLRFELGEEEIKNKKPFQFCWITDFPLVEWNEEEKRWDSVHHPFTSPRE
                     EDLELLDSHPERVKSKAYDLVLNGYEVGGGSIRIHRMDLQRRIFTLLNLKEEEIEQKF
                     GFFIEALQYGCPPHGGIALGFDRLVMLMNGENSIREVIAFPKTQKGVCLLTGAPAPVE
                     EKQLEILGISLQKQEER"
     gene            22218..22919
                     /locus_tag="PK016_02730"
     CDS             22218..22919
                     /locus_tag="PK016_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HXL02022.1"
                     /translation="MRGLRGLNDLWETARNVLPLVIALVFFQLVILRKPIKSVKEFAI
                     GVLLSIFGLHFFLKGISMSLLPLGDSVGENLVVLERKWLMVAVAFVIGYFGTLVEPAL
                     KALALEVEEISIGAIPNKVLVHAVAIGFGSGMGIGVYKILNNISYIKVVAPLLLVILV
                     LIFFAPEEFVSIAMDSASATTGPVNIPLNMALAIGLAKVLENVDPLLSGFGIVGLTSI
                     GAVISVLVLGILTRI"
     gene            22936..23259
                     /locus_tag="PK016_02735"
     CDS             22936..23259
                     /locus_tag="PK016_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HXL02023.1"
                     /translation="MDVKCIVAIVERGKADKIVDRAKKAGAQGATILYGRGTGQSEAL
                     EFFNIHIEASKEIILILSEIDKYKPIYDAIVEAGRLDEPGTGIIFTFLVSELLGLHHR
                     EQIKE"
     gene            complement(23328..23414)
                     /locus_tag="PK016_02740"
     tRNA            complement(23328..23414)
                     /locus_tag="PK016_02740"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23378..23380),aa:Leu,seq:cag)
     gene            complement(23682..24080)
                     /locus_tag="PK016_02745"
     CDS             complement(23682..24080)
                     /locus_tag="PK016_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HXL02024.1"
                     /translation="MPSEFMVRTHSQEELVDITNLVKEAVTSSHIQRGVALVYVPHTT
                     AGVTINESADPNVVEDILETLQKIVPAHFPYHHLEGNAPAHIKASMMGSSVSILVEKG
                     NLLLGTWQGIFFCEFDGPRTRQVWVEVRGE"
BASE COUNT         7159 a         3994 c         5584 g         7303 t
ORIGIN      
        1 ggctggatca cctcctttct aaggagtaag aaggaaatag gcggaatatt ttcttgtttg
       61 tggggatgta gctcagctgg gagagcgccg gctttgcaag ccggaggcca cgggttcgaa
      121 tcccgtcatc tccaccagaa gcctcaaaag ccaatttcag ccactttaat cttttcggtt
      181 tctttttctt ctttttgctt ttgttttttc tccttttctc gagggcaagg gatcaaattg
      241 cagtaagcag ctatcatttt cctcattaag aaatctttaa tctcttctat attctatttc
      301 tctatttctc attttctctt tattttgtag tggcattatt ttgtgatgtt tttatgtttt
      361 tctattgaca ttaggtattg tatatagcta ttatgtagta aaagagagag aagaggggta
      421 gaagaatgtt catcaaaaga accagagggg gaagtaaaga aaaacccctt tattatctcc
      481 agttagtaca atcctataga gatgaaacag gtaaacccaa acatgagtaa tatgcacttt
      541 aggaagagaa gaggatttga taactccctt gtttatgtaa tacttcactc tttagtatcc
      601 ttctcataag tggatggtct cctttctata aaatgtactt taccataaag gccatccact
      661 tttttatttt tacgaggata taggacataa tcattggaat aacactaaag taggatttat
      721 ggaaatggat ttagtatctc atgaaggtgt aacccccgag gagattttgc ttttagctta
      781 actcttaccg atgtatgctc aggatgggca gaaaatagag caataaaaaa cacagaataa
      841 ctatcaaagc cccgcgaggg ggttgacttt taccatagtt gtcattaata tttggagtaa
      901 ggatacgaga ggagggtagg ctaacctact atgcaacaag aaacgactga aaaaaagaat
      961 tccagaaact tccattttcg agaaatgaat attttggttg ctcttattct catgtgtttg
     1021 ctaataacta ttatgaaccc tcgcttttta aacgttttta acctccagac gattgcaaga
     1081 caggttgcta tatatggact cctggcagta ggggaaactt tagttatcat tactggaggg
     1141 attgaccttt cggtagggtc tttggtggct cttaccgggg tgttggtagc cctttttatg
     1201 aaaagtggct tgggtgtggt cttttctata atagtagttt tgtttatctc ttctgttatt
     1261 ggacttacgc atggtttatt tgtcaccaag ctcaatgttc ctccatttat aattacttta
     1321 ggaactctta ctgccgcaag agggctggcg tcagcaatta caaaaggatg gcccattatt
     1381 ggtcttccgg aaagtttctt ctttatagga cagggatata ttgtaggtat tccttttccc
     1441 cttataattc ttttaatttt tgctgccgtt gctttctttg tcacgagata cacagtttta
     1501 ggaagacaac tttatgctac tggtggaaat attgaggcag caaggctttc gggagttgat
     1561 gtggatagaa ggagaatgtt cgcttacata tttaattcct ttctggccgg agttacagga
     1621 gtaattcttg cttcaagatt gagtcaggga caagcgggag tgggtagtgg ttatgaatta
     1681 aatgccatag ctgctgcagt gattggagga gcatctcttg caggaggaga agggacaatc
     1741 cttggggctc ttataggagc taccataatg agcgttattt acgacggatt aattcttctg
     1801 gagatttctt cttactggca tgatgtggtt atgggtcttg tgattgttgc tgctgtaaca
     1861 atagatgtct ggagaaaaag gaggagtaaa acgaggtaaa atcttagggg gcaataaaaa
     1921 tgtttaagat ttttgttttt aattttcaag taacacaaaa tgcttaaaaa ggaggtttgg
     1981 tcaaatgagg aagtttaagt tatctttggt ttttgctctt tgtttcttgg ttttgtttgt
     2041 ctctgcaggg tatgctgaag aaattcatgt ggcagtcatt ggaaaatctg tccatcctta
     2101 ctgggcagag gtagagttgg gggtaaagaa agcagctgaa gatctgggag taaaagcaac
     2161 attttttgta cctcaaaagg aggacattca ggctcagatt tctcaaatgg aatcctttat
     2221 agctatggga gttaatggaa tagcaattgc tccttctgac cccactgctc ttgctcccac
     2281 tattgagacc gcaatcagta aaggaatatc tgtaattact ttggataccg atgctccaga
     2341 aagcaagaga ttagcttata ttggaactga caactacagt gccggtaaat tagcaggaca
     2401 agtgatggca cagctcttac ctgagggagg agaggtagct ataggtactg gctctctgac
     2461 tgctatgaac tccttagaga gaatgagagg ttttacggat ggcatagctg agaatgaaaa
     2521 tatagtggta gtgacaaaac ctgcgctctg tgattttgaa gatacaggaa gagcagtaac
     2581 gttggtagaa caggcgttac taacttatcc aaaccttaaa gggttttttg gagtttacgc
     2641 cattaacggt cctgctgcgg caaaagccat taaaaccgca gagaaagtgg gagaagtatt
     2701 agtggtatgt tttgatacta cagcagaaca tatgcaatta ataaaagaag gtgccattgc
     2761 agcaactata ggacagagac catacatgat gggttataag agtgtggagc ttctctccaa
     2821 gatggctgca gaaggtgtgg atgctgcact tgcagaacta cccgaaaaca gaataataga
     2881 tactggagta gacattgtag ctgctgatgc ctatgttgaa accttgcgaa gtttaggggc
     2941 aaatagtgta gaagagtaca gagaaaaact ttccggtttg ggaattcctg tagagggttg
     3001 gtgagaaatt gagaaggggg gggaagatgc cccccttcct tttttaggag gagaataatt
     3061 tgaaacaaga gttattatta aaaatggaga agatttccaa ggcattcccc ggggtacagg
     3121 ccctttctgg ggtggatttt gaggtctata aaggggaaat tttagccctg gtgggagaaa
     3181 atggagcagg caagtccact cttatgaaaa ttttgactgg tgtttttcaa aaggacgagg
     3241 ggaaaattat atttaaaggc aaggaaattg aggcgagaaa cccgcatcac gctcggcaac
     3301 tgggtatatc tatgatttat caggagttta gtcttgtccc taatctggat attgctacta
     3361 atatatttct ggggaatgag cctaccaaaa ggtttgacat ttttgattat gataaagcga
     3421 gagttgaagc tgaaaggtta ttaaagatgg taggtttaga agtttcgcct gatgttttgg
     3481 tgggagatct tacggtggct caacagcaga tagtagaagt agcaaaggca ttagcttcca
     3541 aatcagagtt aatcattatg gatgaaccta cttctgcctt atcgaaggaa gattcggaaa
     3601 ggctctttgg tataatgaga aaattaaaag aagagggaat atctataata tttataactc
     3661 acaggttaga agaggtcttc tcagttgcgg acaggatagt agtattgagg gatggccaga
     3721 gagtgggaga actttcctgt gaagaaacca gaataagtga agttataaaa ttgatggtag
     3781 gaagagatgt aagagttgta cccagagtag gggtaagtgc tggaaacgtt gtcttagaag
     3841 ttcgcaacct ttcccgtcca cctatgatta aagacgtaag cttttctctt aaaaagggag
     3901 aggttttagg gtttgctgga cttataggag caggaaggac agagattatg agagcgcttt
     3961 ttggagcaga ccttaaagct tctggcgaaa tattcataga ggggcaaaaa gtagaaataa
     4021 agtctcccaa tgacgcagta agactgggta tcgggtttgt tcccgaggac agaaaacttc
     4081 aaggtcttat tcttattatg gcagtatatg aaaacatttc tcttcctatc ttacctttta
     4141 tgttttccaa cggtattatt gattttaaag aagagtattc tttagcagaa agtcttgttg
     4201 aaagacttaa tataaaaact cctactattt ggcaaatagt taataacctc tcgggtggca
     4261 accagcagaa ggtagtttta gctaaatggt tagctttgaa gcccagggtt ttaatactgg
     4321 atgaacctac gagaggaata gatgtggggt caaaggcaga gattcatcat ttaattgatg
     4381 agctggcaaa agaaggtatc ggagttatcc ttatatcttc agaacttcca gaaattcttg
     4441 ctttatctga tagaatactg gtagtctcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnga aaaaaagacc tctcttataa tcctctgtaa aaaagaaaag gttaaccaag
     4621 gttaagaaaa acactttcct cagcgcttgt ggtataagaa gatggtttat agtattttat
     4681 aaaagtctct atgggtagta ttgtgatgag gtcactataa aaggatagaa gttaaaaatt
     4741 ctcaatttgg gaaaaaagag tgtcaagaat ttagtataat ggaattaact ttaaaaggag
     4801 cgattgaaat tgtcagttaa tatccagaat gtgattgaca cgattatgcc acctacgctt
     4861 ggtcaagaaa atactgttga taaacttgag tttggggatc cagaaacaat agtgcaagga
     4921 attgctgtga cttttctagc cactcaagaa gtaattgaaa agtcaattaa actcggcctt
     4981 aacctgataa ttacgcatga aggaattttt tacaatcact taaataaaat taaagcaatt
     5041 gagtcagacc ctgtatatca atgtaaacgc gagacgataa aatcaggtaa aatagcccta
     5101 tttaggtttc atgacactgc tcacagatac aaaacagatg ttattatgac aggattacta
     5161 aggcgtctgg gttgggatag ctatgaggtg aaaaagcaac aaacatattc ggtattaatt
     5221 attcctgaaa tgactttacg ggaagttatg atgcatataa aaaaatcttt aagcatagaa
     5281 tatgtccgtt tcattggaga cttgaccatg aaatgcaggc gcattggaat attggttggt
     5341 tacagaggca ctggtgagat gacaatacct ctttttcatc aggaaaacct ggatttgatg
     5401 gtttatggag agggtccgga atgggaaacg cctgagtatg taagagacac aattcaacaa
     5461 gggagacaaa aatcattgct tgtgttaggt catgcggaaa gtgagatgcc tggaatggag
     5521 tatgtagcaa gcattctgag ggaaaaattt ccagatattc cggttcatca tatctctcaa
     5581 agcccccttt ttctagtttt ttgaagcttt tgtcaataaa atcttctttc ttctggttta
     5641 cacctctttt tttcaccgtg cttcttgtaa atatttttca tagagcctct tgtggggctt
     5701 gacttgataa ttgtttacgt tataattgtg tacagctata gtttccctat ggctaaacta
     5761 catataaagg agtgataaaa gtgaaaaggg cagttttagc gtgtctcctg gtgttggttc
     5821 tggcttgtgg aggagtagct tttggtcaac aggtccagat tcctcgggaa gaagcactct
     5881 atgtagccgg atttcagtgg ggtcctccta caactgataa tccctttacc ggaagtccaa
     5941 tgacctttgt ttctaacccc cggcagcata tttggattta tgagcccttg tttacttatg
     6001 atggtctctt aaccggtgat tatgttccca tacttggaga atcataccag tggcgggatg
     6061 atttaactct ggaagtaaag ctgaatccca atgcttattt ccatgatgga gaaaaagtta
     6121 ccgctgatga cgtagtttat gcttataaat ttggccaaaa gtatccagta ggctattctt
     6181 ctatttggga ttggttagaa gatgtttaca aagaagatga ccaaactgtt atttttaaga
     6241 tgaaagctga taaccctaat aagttgatgg tagaagatgg tttaggcgct gtatatataa
     6301 ttcctgaaca tatttgggca aaagtagaag aagatgctgg taataactta gaagaaatca
     6361 gaaaatttga taacaagaac cctgtaggtt caggacctta ccgagtctat tatgagagtc
     6421 ccgagactat aattctagag agagtagata attactgggg agtagtcctt cacgatggtg
     6481 agctacccaa accaaaatat gtcgttcatc ctatctttaa gagtaatgac gaggggaact
     6541 tagcttttga aaatggagaa gtggatgttt ctcagcagtt tatgcctcgg atttgggaaa
     6601 tgtgggagaa taaagggttg cccgtcggaa cctggtataa agagattcct tatcatgttc
     6661 cagcaactat gccttctctg tggttcaacg taaataaaca tcccttagac cttccggagc
     6721 taagaagagc tattgcttac tgcattgatt atgccaaaat ttctgaatta gctatgtcta
     6781 attactctcc tcaggtggaa gcaagtttaa tagtgcctta tggtgcccag gcaaaatact
     6841 ttaataaaga actggtacaa caggaaggct gggaatacaa tccggaaaaa gctgtagaga
     6901 ttttagaaaa cgagttagga gctaaaaaag gaaatgatgg aatatatgta cttcctgatg
     6961 gaacgagatt aggccccttc aaagttgaat gcccatatgg atggacagac tggaatgctg
     7021 ctttagaaat agttgctcaa tcagctaaag ctataggtat agacgtacaa acttccttcc
     7081 ctgattctcc tgtggcctat gataataggc aaactggtaa ctttgatatg acaatgtgga
     7141 caccttctga gcccagccct gctcaaccct ggttgaactt ccagatagta ctttattctg
     7201 gaggagtacc agaatacggg aaaattgcct accgaaactt cggaagatat aaaaatgaac
     7261 gagtagatga gattattgat ttgataccca aaacttcaga tgaagcagag ttgcaaaaat
     7321 tgtatgatga gctggatagt atatatcgta aagatgttcc tgtaatacca ttaatgtaca
     7381 gaccctgggt attctatgaa tacaatgaaa cttactggaa aggattcccc aacgctgata
     7441 atccctatgc tccaggtatg ccttgtgtag gagcaggtat ggagatactg tggcatattg
     7501 agcctgtaaa ataatttagt cgcaattttt attaaagccc gtggatttca ctccacgggc
     7561 tttaataaaa tatagactgc gtagaggtaa ctccagtgag aaaattcaga agatatattt
     7621 tacaaaagct tttttggtat gttctggctt tttttattgc cctgttttta aactttcttc
     7681 ttccccggct tgtgccggga gatcccatat cagttttggt ttctcaaatg atgtctggag
     7741 ctgtatccag tgaagctcag gaaagagttt atcaatcttt tatacaggag tttggactgg
     7801 ataaaccatt ttttgttcaa ttcttcatct atgtcaagaa tgtgttcaag ggtgatctcg
     7861 ggacctcctt cagtctctat cctctctcgg ttaataaaat aatggctgat gctcttcctt
     7921 ggacactttt tttgcagttt ccggccataa ttgtagggtg gatagtaggg aatttcttag
     7981 gagctcttgc tgcctataga aaagggaatt ttgacagaat tttatttcct gtttcattgt
     8041 ttattaactc tataccttat tattgtttgg caataattct actatatatt tttggcgtgt
     8101 atctgcgctg gtttccaata ggtggaggat acagcagaac tctgcttcct ttctggtcct
     8161 ggacctttgt agtagatgta ttgcatcact actttttgcc ttttatatct attgtgctgg
     8221 taacgattgg aggacaggct ataggaatga gagaaatgtc aatatacgaa ttaaacaccg
     8281 attatgttac atactgtaag atgttaggta taggggacag gaaaattcaa agttacgtat
     8341 tcagaaatgc aatgctaccc cagataactg gtctcgctat cagcttagga actatgatag
     8401 gagggtcgtt gataaccgag attgtatttg gctatccagg aataggaaca actttattca
     8461 atggaataag gcggcttgat tatcccctga ttcagggttc gactttaata atagctttaa
     8521 cagtgttggt ggcaaacttc atcatagata tggtatatgg tctagtagac ccccgaatca
     8581 aagccgctca aactgaggga agttgatatg gaaactctga aactactctt aagatctcca
     8641 aaatttatag taggattagg tatttttgct gttttgtttt taaccgcgtt tatatatcct
     8701 gcagtggttc ctaagaaccc ctttgaaatg gtagggctca tgtatgaacc cccttcttca
     8761 agatacctac tgggaacaga caattttgga agagacacgt ttgtggagct aatacatgga
     8821 atgaaaactt ccttgataat aggtttgttt tcaggaatca tagctacggc tataggggta
     8881 actattggtc tttttgccgg atataaagga ggtaatgtag ataatatttt gaactccatt
     8941 accaatatat ttttggttat accaccgttt gtcattttga tactgataac tgtgagcttg
     9001 agcagtcggt ctcttgtagt aatggggttg gtattgggga ttacttcctg gccctggacg
     9061 gctcgggcag tgagagctca gtctatgagt ttgcggggta gagaacacgt tgatatagca
     9121 agactcaatg gggcaagtgt cgtggaaata atatggagag agattatgcc ctatattttg
     9181 tcttacatat ttatggcttt tgttctccaa gtggcaagcg gaatacttaa cgaagcaggt
     9241 ataagtatgt tggggctcgg tcccagtaat gtagtttcac ttggtactat gttatcctgg
     9301 gcgcttttat ttgaatcggt gagatccgga gcgtggtggg cttttgtgcc tccggcaata
     9361 actatagctt taatcacttt ctctctctat tttatgaata ctgggatgga cgaaattttt
     9421 aatccccgat taaggagcta gaagtggaag aatccaggaa aataatatta gaaataaagg
     9481 gtctcaaagc atattataga acaaggtcag gagaacgcat cagagcagtt gatgatgttt
     9541 catttggttt gatggaagga gaaatattag gcatagcggg tgagtcaggt tgtggtaaat
     9601 cgacattggc tatggctatg tccggtcttt ttttacctcc tctaacttat gaaagtggca
     9661 cagtgttttt agatggtgaa aacataatag gtaaaaaaga agaagagttg aggaaaaaaa
     9721 tactgggaag gagatactcc tatatacctc aaagtgctat gaatgcatta aatcctactt
     9781 taagaattaa aaattttgtt atagacctct taaaagaaca tcatcctaac atgagcaaaa
     9841 aagaagtttt agatttaacc acggagagac taaagtcact ttctctgcct ccctgggtga
     9901 tagaccttta tcctctggag ctaagcgggg gtatgaaaca gagggtagta atagcagtgt
     9961 caacacttat gaatcctaaa gtggtagtgg cagatgagcc cacttctgct ttggatgtaa
    10021 cctcacaaaa gatagtgata aagttgataa aagacctttt tgacgaagga atagtaaaaa
    10081 gcgtgatatt cataactcat gagttaccta tattgagaca cgtatgcgat aggatagcag
    10141 taatgtatgc cggagaattt gtagaaatgg ggaaaactga agatattata tttaatcctc
    10201 ttcatcctta ttctcaagct ttgatgcaat ccatattagt gccggaggtg gggataaaag
    10261 gtaaaaagct tcctaactta cccgggtcac ctcctgatct aagaaaacca ataaaagggt
    10321 gtagatttgc tgatagatgt tatttagcaa tagaagattg ccggaaagaa actataaaca
    10381 tgacggaagt gggcggtaga gaagtgagat gcataagagt ggattacatc aaggcaaaag
    10441 aggaagtggc accctatgtc taataatgat gaaataatta ggacggaaaa tttgaccagg
    10501 gttttcggaa gtggtaaaaa ggctataaaa gcagtagata atgttaatct ttccataaaa
    10561 aggaaagaga ttatctcttt agtggggcaa agtggaagtg gaaaaactac tcttgctaaa
    10621 atgattctta gacttttaaa accgaccacc gggaaaataa tatttgaagg tcaagacgtt
    10681 accaacattg aaggaaaaga aaagaaagct tactggagaa aagtgcaagc tatatttcaa
    10741 gacccttatg cttcttttaa catattttca ccggttaaga aagtactatt ggatgctttc
    10801 aaactatttg acaataaatt taccagggaa gaaaaatgga agatggtatg tgaatcttta
    10861 gaagcggtaa attttagacc ggatgaagtg gcagataaat ttccctttga gctcagtggg
    10921 ggacaaagac aaagaataat gatagccagg gctcatctca taaaacctga gcttttgata
    10981 gctgatgaac ccacttctat gatagatgct aacttgaggt caggaatatt agaactgtta
    11041 ttagatttaa gaaataccga aggaaccacg ataatgtttg ttacccatga cttaggttta
    11101 gcttactatg tgagtgaacg gttattgatt atgcacgaag gaaaaattgt agaagaggga
    11161 agcgcagata aaataatatc caaacctgag catccttaca ctaaacagct gatagctgat
    11221 gttcccaaat tatcccatga gtggttatta gaatgaagca cagtggggta gagaagagag
    11281 ggtatatgtg ttttactttt accctgtagt ttctgatgtt tttatgctgt aattgccagg
    11341 ggtgtatatg ttttatcaca atacccctgg attatatttg cgattttgta acataaatta
    11401 tataatagct ttcttacctg tcgttatgaa cctcgttttt gtgtgttggc gacccttctc
    11461 tcccaatata agggtcgctt ttcttgtttc cggtagtagc ctggcttttt agaaaaaaag
    11521 ttattctatt actattattt atttcttgca aaaacggaaa aaaagaagct ctggaagtat
    11581 tccgtgttta gaaaataagg tttggataaa taaatttttc ataaaattaa gcattgatta
    11641 tatagaaagg gaggaatgtt tggtggcaaa actatttcca gaagattttg tatggggagt
    11701 ggcaacggcg tcataccaga tcgaagggtc acctcttgct gacggtgcag gaccatcaat
    11761 atggcacagg ttctctcaca ccccaggcac tattttaaac ggggataccg gagatatagc
    11821 cgatgaccat taccgtagat accgagaaga cgtagctttg atgaaagaat tagggttaaa
    11881 gggatatcgg ttttctatag cctggtcaag gatttttcct tatggtaaag ggaaaataaa
    11941 tgaaaaagga ttggattttt ataacaggtt agtggatgag ctggtgagcg ctggtataac
    12001 tccatttgtt actctatacc actgggattt gcctgctgcc ctccaggatt tggggggatg
    12061 ggctaataga gatatagcct gttggtatgc agattacgct gattttatgt tccagaggtt
    12121 aggtgataga gtaaaaaact ggataacatt aaacgagccc tgggtagctg cttttgtagg
    12181 ttattatgaa ggaagacatg ccccggggat gagagattta tatgcggcgt ttttggctac
    12241 ccataatcaa ctgagagctc attctaaagc agttagagca tttagagagg aaacgagagg
    12301 aggtaaaatt ggtataactt tatctaacag ttctcgtgat ccggtcagta gctcgaaaga
    12361 ggataaggaa gctgctcaac ttgctcacga atgggctaat tatccgttat ttttacaccc
    12421 tatatacaag ggagactatc cggagaaagt tcgagagcgg gcaagagaat ttttgcctcc
    12481 ccattacgaa aaagatatgg cagaaataaa agagcctata gattttgtag gtataaatta
    12541 ttactatggc gttctggttc aggcagaccc tcaatcacca ctggggggaa aagcagtgga
    12601 aaggggatta cccaaaacag agatgggttg ggagatatac ccagaaggtt tttataagat
    12661 attaaaaggg gttcaaaatg aatatgaccc ggcggaagtt tttgtgacag aaaacggagc
    12721 agcatttgag gataaagtgg aagatggaaa agtacatgat gaaaagagaa tcaactattt
    12781 acaagaacat tttgggcaag cattgaaggc aatagaggat ggggttaagt taaaaggata
    12841 ctttgtatgg tcactattag ataactttga atgggcttta ggatactcga agagattcgg
    12901 tatagtatat gtggattatg agactcagaa aagaataata aaagatagtg gctattggta
    12961 ttctaaggta ataaaaagta attctattga ataaagtagg aaggaaggct ttagttttac
    13021 ttgacccttt atcaaaaagg gagaaaaatc gggaagttta taagttagat aatgtttatt
    13081 ttgttttttt gaatataaaa aagtgaatga ggagagaagc tatggctaaa atcaaagtag
    13141 gggtgattgg agcaggtaat attggtgtgg ttcatgctct tgcctataga gacgttcctg
    13201 aagcagagct ctgggctatt gctgatatcg attctgagcg cctttttgag gtgcaaaaga
    13261 ggttagaaat tcccaaagct tacactaatt atgaagacct tttaggagat ccagaaattg
    13321 aggcggtgag tatatgttta cctaattttc tccatcaccg ggctgcttgt gctgccttag
    13381 aaagtggtaa acatgtactt ttggaaaaac ctatggctat gaatgctaaa gaagctcatg
    13441 agattgttga ttgtgcaaag aagaatggca aaattctcat gattggtttt tcttatagat
    13501 ttaggtcgga agttataacc cttaagtcct gggtagaaga gggtcttctg ggagaagtat
    13561 atcatgctca gtctattatt ttgaggagac ggggtatccc ggctatggga agctggttta
    13621 ccacgaaagc aaaatctggt ggtggagcac tgattgacat aggagtccat tatttagacc
    13681 tggggatgtg gcttatggga tatcctgaac cggttagcgt gagcggcttt acttttgata
    13741 aaattgccac tcgccagcct tatatttatg tggatatgtg gggtgaaccc gtgcctggtg
    13801 ggccttttga tgtagatgag tatgctatta gccttgtccg attccaaaat ggggctgctc
    13861 tgtggatgga gagtagctgg gcactcaatg ctcaggatga ggatagagtt cagatttttg
    13921 gagataaagc gggagcaaaa ttgagctcta aggaagggct tactctcttt ggagaattgg
    13981 gaagtaaaat tgctgatgta aaaccccaaa ttaagtcaca aaccccaggt gctatatata
    14041 gagaagaggt tcttcacttt ttgcagtgcg taaaagaagg taaggagcct atggcttctg
    14101 gtgaacatgg actcattgtt cagaaggtaa ttgatgctat ttatgagtct gaaaagcaac
    14161 aacaagaaat aaaaataagc taaaattgga aaggagttca agaaatgaga ttggcagtgt
    14221 cttcatggag tttacgcgat cacgtgaata gagattttcc tcttcaagat tttcccagag
    14281 tgataaaaga gcgttatggt gtggaagcgg tggaactttg ccagatgcat tttcgagctc
    14341 aggattctaa atatcttgat gaaataataa acggactgaa agcagctggg tctgtggtta
    14401 taaacatgcc catcgatact ggaaacatat ctcaactgga tgagcgaaaa cgtaatcacg
    14461 atattaaaat tatcgaaggc tggatggatg tggcagcgta tgtgggttct cccaatatca
    14521 gagttaatac tggaagtcag aaccagggag ttgtggattt atccataact gtagattctt
    14581 atcgaaagtt agcagaatat ggcgaaaaaa ttggagttat ggtgcttctg gaaaatcatg
    14641 gaggaatatc agcagatcct cacaacatag taagattatt cgaagaagtt ggacataaaa
    14701 actttggagc ctgtcctgac tttggaaatt tctcacccga ggtacgctac caggggctgg
    14761 aaatgatggc aaaatatgcc gtaatagctc atgctaaaac ttatgatttt gatgaagaga
    14821 ctaaaatgtc tgaagaagat tttgtgaagt gtctgaatat tttaaaatct gcagggtttg
    14881 atgggtatct atcggtggaa tttgaaggta aaaaagacca gtattctggt gtaaagcggg
    14941 caattgaact tattaaagta tcctgcaaaa ctgattaaaa tgttaaaaac tttaaaaagt
    15001 aaattatatc gaaagtcaaa aactcaaggt tgttacacac agagaaagac tttgtctttc
    15061 tctgtggttc ttgtcttttg tgtttgtttt tgagtttttc ggggtttata agggggcttt
    15121 gcccccttat agtttgtaat ctttatcatc tattgtgcct ttatcttacg tcgtagacag
    15181 tgagggatac tataattttt ccagtagtct tttgctttta ttttttgttt ctttgttctt
    15241 tgtcttttgg gggactataa aggggtgctc ccctttatag tattcgtaat ggattttgcg
    15301 gttaactata aattatagct gtcaaagtca gggaagaagt ggcggagaaa agactctgaa
    15361 acgatactgc ttatcaatgc cctcatagag ggcattgata agcagtgagc aatttacctt
    15421 tttacaggtt ctaagaaagg caacatgtat ttaaagttgg gaaaatggtg gtagggttga
    15481 ctgtatggat tttcggcacc aggataatta gtccagtagt actcatccca gcctaccact
    15541 cctggataac caaatgtggg aatagtgggc atttcttcta taagtatttt taacccttcc
    15601 atacccagtt ccatatttcg ggggtcattc caatccacct tttccatttc ttcaacaatc
    15661 tcatctagcc gggaatctgt ccaccttgag acatgtcctg gtgtattttc ccctataggt
    15721 cgatagaatg aagagtagaa aggagtgaat gaacgatgta agtcagggtg tcctccccag
    15781 ggctctgttg ctggccataa atcggtcact tcaaaatctc ctaccccggc cagagttccc
    15841 tgagcttctg aggttctcat ctctacgtca attccaaaat ttctccactg ctgggtggca
    15901 gcaagtgaat ttctgtgttg cccgtggaca ggattgggat gtgctaatat gacgattttc
    15961 cagggagttc catcggggag gagccacttt ccgttttcat ctctggtgaa cccatttctg
    16021 agtaatagtt gttctgctac atcgggagca tatttccacc atcccggtcc aaatatttct
    16081 ttgatttgtt cgggatcctc tgatacgcta tagcctctct cttttgcata ttcaacggct
    16141 cttagtggtg cgtcagggtc ataaggttta aagcgttctc catttacctc aatatctaaa
    16201 gtaaagttct tcaaccattc ttccattggt tcataatacc acttttgata cacaggtgtg
    16261 ggaggtacat gtaaaactcc catagtagct gctccatcca gtgcgattgc aatgtagtct
    16321 actatatcta tagctaaagt caaagcccac ctgacgtctt ttaaattgaa cggatatgcc
    16381 tcggtgttaa aagttatacc cgtgatacag gggtctgtgt ttactaccca gggatattct
    16441 tttctgtatc ctcttgaata tggattttta cttatgacag ccctgaaccc ttcaatatct
    16501 aaatctgcta tgtcaaggtt gtggttgacc tggtcaataa ctataggagt tacctcgcca
    16561 taagagggga aaagaacata tttaggtttg ggcataccat acaatattcc ggtgggagtt
    16621 ttatcccagt cttcccttct ctcccagagt acccagtatc ctgctggatc gtaacttttc
    16681 agtatatatg gaccactgct tacgggagga ttaaactcaa aagataccgg gtcttctacc
    16741 ttttcaaaaa tatgtttggg gaaaggtctc cacgctcccc acctatctac aaaattggtg
    16801 tgaaatcgag cattaggttc tttaagctca aaaactactg tgtagtcatc agttttgtag
    16861 actttttcta cataaagatt aaattgagca ttgtaattca tccccgggta tttcatagtt
    16921 gtttctattc cgtatactat atcatctgca gttatcggga ctccatcact ccagtagcaa
    16981 ccttctctca gttttactgt catctgagta aagtcttcgt tgtaaatagg gccttctgta
    17041 gctaaggcgt ttataatctc gccaaggacg tgttcattca tccataatgg ttccaacatt
    17101 agctggtgga ttccgcactg tacgtatctc caggtagtag taaacacgtt aaagtttccg
    17161 gcagtggcta ccctcggtcc tggacctgta gctatcaatg tttcctctct ggggatatct
    17221 tcagctaact gggcgaagcc cgtttgagta aaacccatcg ccagtatcac taaagagaca
    17281 aacaaaatca aagaactttt tttcattagt tacacctcca gtttgaatat ggttttggtg
    17341 ctttttatca gagttttggg ttgtttttac ttcacctcct ctcctattta cccccgcttt
    17401 aaaatgtatg aagattgttg aagtggaagt ctaactttta tttttcatgt tctgtttgtt
    17461 tttttgatat actttgcttc atcttcccta ttatactgca aaaaataatt ttctctaaga
    17521 gaatcttttc tactttggta agaaacatcc tttcctttgt gaaaaagttt catttctgtc
    17581 atgcaagacc tcttcttgaa ggcgagagca atttagcagg ctggtgttaa atgtccatta
    17641 atttcgaata tactttcctg agtggatgaa gctttcctcc attttcagaa ggaggagagc
    17701 tatttagaaa agtttcctga aaaggcatct cctcaaagcc tgttcttaag acttttactg
    17761 cagaatattt cccccggggg tagcaattat agtagaatat attgcagaat agagttagaa
    17821 gtgctgtggg ggtgaaattt tgtctaagcc aaaaatttta gtagtgggaa gcttaaatat
    17881 agacatgata cttaaaatcg agcggatgcc cgaagtagga gaaaatttgt tcgtcaaaga
    17941 attttctatg cttcctggag gtaaaggtaa taaccaggct gtagcctgtg ctcgtctggg
    18001 agcactggta agtgtgattg gtaaagtagg agatgacgag tttggagaac ggttatttgc
    18061 caatctggag caggagaatg tagattttgc ttttgtcact agacaggctt tgacccatac
    18121 tggccttgct tttatatttg tagatgaaaa aggggaaaac cgaatcctgg tagctcctgg
    18181 tgctaatatg gagctttcaa ttactgatct ggataacgct ttagagctct tcaaagctaa
    18241 tgaattttta gttttacaac tggagatacc ccaggaagtg gtgaaaaagt gtatcgagta
    18301 tgctcaccgc tttggtatga gagtagtact caatcctgcg cctgctcagc cttttcctat
    18361 agaaattttg actgccaatg atataattac tccgaataag tacgaggcag aagttttaag
    18421 cgggattaaa attaagagtc tcagtgacgc cttgaaagct atccgggttc tggatgaacg
    18481 ttgtccagct cggaaggtga ttaccttagg aggagaagga gcagtggcga gctatgaaaa
    18541 taatcagttc gtctatctcc ctgcccgaga ggtaaaagtg gaagatacca ccgctgcagg
    18601 agatgccttt aatggtgggt tggtggtggg aattggtatg ggaaaagatt ggataggggc
    18661 tctgaaactt gctaataacg ctggagctat tgcctgtact aaagtgggag cccaaagcgc
    18721 ccttccccac ctccgggatg tggagagatt ggtcaaaaaa ataggggagg gcgaaataaa
    18781 gataattttg ggagattgaa ctttccctcc ggggaaagca agggataaaa gaggaggaaa
    18841 gtgatgagca tcattaaagc ccccaaaggg actcgagata tatggggaga ggaaatttat
    18901 ctctggcacc gagtagaaga agtggttcgt tctctggctt cttcttttca atatgaggag
    18961 ataagaaccc ctatttttga aagtaccgac ctttttgccc ggggagtggg ggaagttact
    19021 gacattgtgc aaaaggaaat gtataccttt ccagataaag gaggacgtag ccttactctt
    19081 cgtcctgaag gaacagctcc agttatgaga gcttacttgg aacatggttt aaatatttct
    19141 aaccctcgag ttaagtggtt ttatatgggt cctatgtttc gttatgaacg acctcaggca
    19201 gggcgaatga gacagtttca ccaatttggc tttgaggcta tcggttttcc tcaacctgcc
    19261 tgtgatgcag aaatcattct tatagcctgg ctggtaagca aaaaactggg attggagaat
    19321 ctctctttag aaattaacag tctgggttgc aataactgta gagacaaata tataaaagtt
    19381 cttcaggatt tcttaaacag agaagaaaat aatctctgtg ctaactgtaa aacccgaaca
    19441 gagaagaatc ctctccgggt tctggattgc aaagaggaaa cctgccagga agttattcat
    19501 tcttccgttt ttcccgtggt gcaagatttc ctatgtgagg aatgtcgaga gcatcaggag
    19561 aatttgatgg agatcttaaa agaactgggc attcctttct ctatcaatcc ttatctagta
    19621 agaggtttag attattatac gagaactgct tttgaaatta aaaccgggga attgggagca
    19681 caagatgctg tagcaggagg agggaggtat gacaatctat cttcctcttt aggaggtcct
    19741 cctgttccgg gggtaggttt tgctgccgga atggaaagga tgattcttct tttagctaaa
    19801 aaatatgaac cttcttcttt taaggaagag cccctgtttt atcttgctcc cttagatgcc
    19861 ccgggagaga aaactctgct acaattggct tcatttctgg gagagagagc gattcctttc
    19921 tatttagagt ttgctgataa aggagtaaaa tatcatctta agaaggctca aaaaatgggg
    19981 attaataacg tgattatagt aggtagtaaa gagagagata gggaagagtt tatctggaaa
    20041 gatatgagag tgggagaaca aagagcgatg aaaagggaag agctattagc cacattattg
    20101 gaggaaagag agaaatgttg aggactcaca cctgcggtga attgagaaaa gaagatgcgg
    20161 gaaaggaagt gcaattaact ggctgggtaa gtcgtttgcg agaccatgga ggaattttgt
    20221 ttatcgattt gcgggataga tggggtatta cccagctggt ggtagataat aaggaggaaa
    20281 tttataatcg agcttcccgg attagaccgg agtatgttgt gaaagtaaaa ggagtggtaa
    20341 aagagcgccc ggaagggctg gttaaccctc ggatttctac cggagaagtg gaggttgcag
    20401 tagaagattt agaagtttta tctacctcag agttgcctcc ttttgagata gagggcaata
    20461 aagaaacaga cgaatctatc cgccttcgtt atcgctattt agatcttcgc cgggataaaa
    20521 tgcagaaaaa cttaatcctc cgtcatcgtg tagctcagct aatacgccgg tttctttctg
    20581 agagagaatt tctggaagta gagactcctt ttctcactaa aagcactcct gagggagctc
    20641 gggatttttt ggttcccagc agactaaacc cgggccattt ttatgctctt cctcaatctc
    20701 ctcagctttt taaacagatt ttgatgattt ctggtttgga ccgttatttt caaatagtta
    20761 agtgttttcg ggatgaagac ctgcgagctg atcggcagcc agagtttact caggtggata
    20821 tagaaatgtc ttttatagag ccagaagaca taattcagtt gatagaaggg atgatgggtt
    20881 atgtttttaa ggaatcttta ggagtagcaa ttgaaactcc tttccctcga ctttcctatg
    20941 atgaggcgat ggaaaaatac ggtacagata aacctgattt gcgtattccc tgggagattg
    21001 acgatcttac tgaacttttg ggaagagaaa taacttcttt ttctcgggga gggaataact
    21061 ttgcggttaa aggtcttttc tttccctcgg gggaggagtt ttctcggaaa aaactggatg
    21121 ttctttccca gaaagcaaaa gaaagttctc tttctctatc ctgggttaaa ggaggaagct
    21181 ctgctctttc ctctcccctg aggggcaaac tttctgagga gtcctggcaa gcgatagagg
    21241 aaaaatatcc ttttggagaa aataccctgc ttcttttagc ctggggagag aaaactgatt
    21301 tggtgaattt tatgggtggt ttgcggtttg aactgggcga agaagagata aaaaataaaa
    21361 aaccttttca attctgctgg ataactgatt ttcctctggt agagtggaat gaagaagaaa
    21421 aacgctggga ttcggttcat catcctttta cttcacctcg ggaagaagac cttgagcttc
    21481 tggatagcca tccggaaaga gtaaaatcta aagcttatga tttggtcttg aatggttatg
    21541 aagtaggggg ggggagtatt cgtattcacc gcatggattt gcagaggaga atttttactc
    21601 ttttgaattt aaaagaggag gaaattgagc agaagtttgg tttctttatc gaagctctgc
    21661 aatatggttg tcctccccat ggggggattg ctctgggttt tgaccgattg gtgatgttga
    21721 tgaatgggga gaattctatc cgagaggtta ttgcttttcc caagacccag aagggggtat
    21781 gccttctaac tggagctcca gccccagtag aagaaaaaca gctggaaatt ttaggtattt
    21841 ctcttcaaaa gcaagaggaa cgatgattgt ctataaagtt tccgggttat tgagtaagtc
    21901 tacccatgta atctataagt aaatataaaa gttaaaattg taaggagagc aaaagaacca
    21961 ctttaatatt aatagagggt agttctttct ttctctattt gtaccataat aatccctgag
    22021 taaacacctc cgaaaaagta ttttgagata ctaatataca gataaagcaa gaagactatt
    22081 gggagaccac agtccatctg taaaagcgga gtttagaatg acagggtata agtagattaa
    22141 tattgagggt tggtttatac taatagaagc tgcatgtgtg tttggcgtaa agattatact
    22201 cccgaaagga gcacaatatg agaggattaa gaggcttgaa tgatttatgg gaaactgcaa
    22261 gaaacgtgct tcctctggtt atcgctcttg tttttttcca attagtgata ctgaggaagc
    22321 ctattaaaag tgttaaagaa tttgctatag gagtcttatt aagtatattt ggcttacatt
    22381 tttttctaaa aggtatctct atgagtcttt tacctctggg tgattcggtg ggagagaatt
    22441 tagtagtatt agagcgaaaa tggttgatgg tagctgtggc tttcgtaata ggatattttg
    22501 gtactcttgt ggagccagct ttaaaagctc tggctttaga ggtcgaggaa atatccatag
    22561 gagctatacc taataaggtt ttggttcatg ccgttgctat tggatttggc tcgggaatgg
    22621 gtataggagt atataagata cttaacaata tatcttatat aaaagttgta gcacctttgc
    22681 ttttagtaat tctcgtatta attttttttg caccggagga atttgtatct attgctatgg
    22741 attcggctag tgccacgaca ggtccagtca atattcccct taatatggct ttggcaatag
    22801 gtttagcaaa ggtactggaa aatgttgatc ctttattatc tggttttggt atagtgggac
    22861 ttacctccat tggagcagtg atatcggttc tggtattagg aatattaacg aggatataaa
    22921 gagaggaaat attatatgga tgttaaatgt atagttgcca ttgtcgagag aggaaaagca
    22981 gataagattg tggatagagc aaagaaagct ggagcacaag gagctactat tctttatgga
    23041 agagggacag gacaatcaga agccttagaa ttttttaata tccacattga agcatcaaaa
    23101 gaaattattt taatactcag tgaaattgac aagtataaac caatatatga tgctatcgtt
    23161 gaagcaggta ggcttgatga accaggtact ggtatcatat tcacttttct ggtatctgaa
    23221 ttgctgggat tacatcatag agagcaaata aaagaatgat tagtggtcgc gctttttagc
    23281 tattgggttt gtacccggga aagagaagca accgcaaaaa acttttctgg tgccgaaggc
    23341 gggagttgaa cccgcatgag ctttcactca ctggagcctg attccagcgc gtctgccatt
    23401 tccgccactt cggcaaccca gagtaaaata tcttaaattt ccttacttgt caagattaga
    23461 tacggcagca atagcgccaa ctcattgtag gagtatttct gtgtattttt tacttttccc
    23521 tccatttttg atttgggatt tattctttct caacctccag ttttcatctt cagagataaa
    23581 aattcctata acgttagtct aaataacctc atagactaac ttaattggat atcttcttaa
    23641 gaaggacata gtgtcgtctg cttctattta atagtagtaa gctattcccc tctaacctct
    23701 acccacacct ggcgagtgcg ggggccatca aattcacaga aaaaaattcc ctgccaggtt
    23761 cctaaaagta gattgccctt ctctactagt atcgaaacag aagaacccat catgctggcc
    23821 ttaatgtgag caggagcatt cccttccaga tggtgatagg gaaaatgagc aggaactatt
    23881 ttttgcagag tttctaaaat atcctctact acattggggt cggcgctttc gttgatggta
    23941 actccagcag tggtatgggg aacataaacc agtgctaccc ccctttgaat atgagaagaa
    24001 gtgactgcct ccttaactaa attggtgata tcgacaagct cttcttgaga atgagttcta
    24061 accataaatt cactgggcat ttttatacct cctattctgt aaacggcaat aatggggggc
    24121 gaagcccccc ttataacccc
//
LOCUS       DAOSVE010000018        23558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_1403_length_23558_cov_71.440157, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000018 DAOSVE010000000
VERSION     DAOSVE010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 23558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23558
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1403_length_23558_cov_71.440157"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(404..1321)
                     /gene="ftsY"
                     /locus_tag="PK016_02750"
     CDS             complement(404..1321)
                     /gene="ftsY"
                     /locus_tag="PK016_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HXL02025.1"
                     /translation="MMEKETIWQRWVKGMGKMKEEVKEHFSQLWSKKEVGEKDWEELE
                     ELLIQADMGPALSLEIVEDFREFKKSQREGDWQEWLYQKLFSLLQGNDPSLFRSAEEG
                     ELKAIILSGVNGVGKTTVAGKLAYLFKNKGEKVNLIAADTFRAAAMEQLEIWAQRAKC
                     HYFRGTEGADPGAVVFDGINSARHQGDTLVIIDTAGRSHVNRNLLAELEKVVRVTKKL
                     ILPSNLESLLVIDALAGQNAFLQAQSLFPVADLSGIILTKWDSQAKGGIVFRIGKEFH
                     LPVKYLGVGEGIEDLVSFDAEEFVKTIVY"
     gene            complement(1333..4452)
                     /locus_tag="PK016_02755"
     CDS             complement(1333..4452)
                     /locus_tag="PK016_02755"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007076 - mitotic chromosome condensation
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation SMC family protein"
                     /protein_id="HXL02026.1"
                     /translation="MYVKKIIIQGFKSFAHFTAIELSPGLNVVVGPNGSGKSNLIDAL
                     RWVWGDTARELRVTQNREVIFHGSRTAKPLGMADIEVWWEDSSGADLKVARRIFASGE
                     SEYFFRDEKIRFRDWKEKLEKKGLGIERLSAGVVGAGELQSLLYETPLERWQWLEMIS
                     GAGEWKKKLSQVSLRIEKLESRRKLLEERLKELHIQLEKWKLWAQEEEEYLSLEREWR
                     DTKALYIQEMIRLHEEKNSLLKKEIETEEEKIAEGEKEERKWKDIFSSLSIVRDKCQR
                     EKKDIERERGEKEREKKKEEEKLYYLLTEMRENRKNILRIKEKFSSWERTVNLWEERE
                     KKWREDFSFPEDKVEEIDDEELMLQLEERANTLRELEKKKIYLQECKRHGEEDKKKLE
                     ASLQDEIRREKRIGEEILELKTKIKKGEENIGEVENKIERGKEEEKILREKLEKGKVI
                     LQSISRKLNNLKQRKQGSEEWEKELSVLGWSRRAVQALNWFWESQNIKEEGFDSEPDL
                     PGSKQWYISSSAIPPALWRECQKREVFHLFQSGKLPEENLVSSDGNLVFLRSGFLLFP
                     GQLISSVGESHFYHSWKKREKKWQDKVAFWESRIRDISKEEKNEEEKLWKRKLEIERE
                     KEKLVYREKEKEERERKIRELEKEIELVAGKKNAEEKELSVTEEEIVRIKENISQIQC
                     SIKEREKDKRKKEEREREKERFLWEIQSLKKEIGEALEVLKVEEEEKRKYGKRLSEEG
                     SKIQVIQVSIAEKMKEQKQKEEEEKSLDGKISLAEKKIQEIEKEKEERRKKLDKALFL
                     EEKLDLEVKELSQELEKMGNFRPAPSSICIDIKELERSLQEKEELLGKKKFIPGAGEE
                     FVRVEERFSSLKEKNECCEAGLSLVREGVSLARREIRKSFQEFLYQVNKSFSGFFKEI
                     FPGGEAYLLFQEEKAEIEVRLPGKRKQILSLLSSGEKSLVALCFLFAVLETANLPFCF
                     LDEVDANLDHANSLLLAQLLKNISHNRQVVVVTHQEEVMEVADRIIGMTMNEPGVSQA
                     VCFDGESFQ"
     gene            complement(4452..5159)
                     /gene="rnc"
                     /locus_tag="PK016_02760"
     CDS             complement(4452..5159)
                     /gene="rnc"
                     /locus_tag="PK016_02760"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HXL02027.1"
                     /translation="MFSSDIDFSVLEERIRYVFRNEELLKIALRHKSALEGKDGSCND
                     KLEWLGDAVVGFFVTDYLFRHYDKPRSWLSLTKSKWVSEECLAYLARKIELAKFIELG
                     KGEERSGGREKDSILASSLEALAGAVFVDSSSYEETKKVLKTIFCEAGDIDSLNLSVN
                     YKGLLQRWCLQYYNCLPHYQILNQGEENLYQIEVKIGENSIARGEGKNKKRAEQEAAK
                     KALEVLSSDNQLLNKGQ"
     gene            complement(5171..5404)
                     /locus_tag="PK016_02765"
     CDS             complement(5171..5404)
                     /locus_tag="PK016_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007555299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HXL02028.1"
                     /translation="MDIFSKVKEIVVEQLGVDEEDVTLDASFIDDLGADSLDIVELIM
                     AFEEEFDIEIPDEDAEKITTVGEAIEYIESKIE"
     gene            complement(5432..6178)
                     /gene="fabG"
                     /locus_tag="PK016_02770"
     CDS             complement(5432..6178)
                     /gene="fabG"
                     /locus_tag="PK016_02770"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HXL02029.1"
                     /translation="MSLENQVAMVTGGRRGIGFSIAQVLGRGGARVAINDVAPQEEIE
                     KAVTELKEQGIEARGYVADVTKKEEIKTVVQDIIKTWGQVDILVNNAGITRDALLVRM
                     KDEDWRAVLDVGVQGVYYCTKEVLRYMMKKRYGRIINISSVVGVVGNAGQTNYSTAKA
                     AILGFTKSLAREVAPLGITVNAVAPGFIDTEMTEKLPEEVREVWLQQVPLRRFGEPEE
                     VAQAVAFLASRAAGYITGQTIHVNGGLVMI"
     gene            complement(6175..7116)
                     /gene="fabD"
                     /locus_tag="PK016_02775"
     CDS             complement(6175..7116)
                     /gene="fabD"
                     /locus_tag="PK016_02775"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HXL02030.1"
                     /translation="MGWVVLFPGQGAQKAGMTKIWWENYRQETEEILGKASQICGVDL
                     LHLTLEGPEEELSLTFNAQPAILATSLLIWRIWQREFSLPVDAVAGHSLGEYSALCAA
                     GSIDFEEAIFLVRKRGELMQEAIPPGEGAMVAVLGLPQEKVENVVEEFSSGDKVEIAN
                     INSSSEVVLSLSRSLLPQVVDRLKKEGSKKVVELKVSAPFHSSFMKEAAEKFSSLLQE
                     IEIKPPRYRYVSNVNAQYVSRPEEIRDLLVKQMFSPVRWKEVMDVLYRGESHQVWEMG
                     PGTVLSKLFSREYPEVEVKNVNSWDGGRKENKEEKEK"
     gene            complement(7118..8065)
                     /gene="fabK"
                     /locus_tag="PK016_02780"
     CDS             complement(7118..8065)
                     /gene="fabK"
                     /locus_tag="PK016_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HXL02031.1"
                     /translation="MMRGRVTEILGIKYPILQGGMAWVSTAPLVAAVSEAGGLGIIGA
                     GGMTGEGLREEIRRTTSLTGQPFGVNLMLLSTYVKEQIEVVKEEKVPVVTTGAGNPGE
                     LVEDFARRGILTIPVVASVALAKRLLRRGIKLIIAEGMEAGGHIGEITTMCLVPQIVD
                     AVSPLPVIAAGGIADGRGMAACFALGAEGIQMGTRFVCTEECEVHPLYKEAIVKAQDR
                     STVVTGNTTGHPVRCLRNRLTRKFEELEAQRAPLAEIEALGSGRLREAVCGNVEEGSV
                     MSGQVAGLIKDIKPVRQVIEDIIAEFKETVKKLPQMVDD"
     gene            complement(8072..9058)
                     /gene="plsX"
                     /locus_tag="PK016_02785"
     CDS             complement(8072..9058)
                     /gene="plsX"
                     /locus_tag="PK016_02785"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HXL02032.1"
                     /translation="MRIAVDGWGGDFAPEEIIKGVREAYSPDYTLVVVGPRQKLLSLY
                     SQWGMDEEHFPIEDASQIVGMGESPTEALKKKPDSTIAKGIDLLAQGKIDAFISAGNS
                     GAVMATAWMKLKRVSQIERPAITALIPNSNSCTVLLDVGANVDCKPKHLLHFAVMGAE
                     YAKIVLNLPHPRVGLLNIGEEEGKGNELVKSTYQLFRSQSDFLHFDFAGNVEGYNIVD
                     GKADVVVCDGFTGNALLKFGEGLIALISSHLPQEIGSPQEEEKLKEFWRKLDRSEYGG
                     APLLGIEGVCLICHGSSKARDIKSAIKGAKELVEKGIVDKIRERLSQLNLKS"
     gene            complement(9071..9250)
                     /gene="rpmF"
                     /locus_tag="PK016_02790"
     CDS             complement(9071..9250)
                     /gene="rpmF"
                     /locus_tag="PK016_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HXL02033.1"
                     /translation="MANLTNKTSRCRRNKRRTHWVVKAPQLVDCPRCHQPKLPHRVCP
                     ACGYYKGKQVVKVKD"
     gene            complement(9349..9891)
                     /locus_tag="PK016_02795"
     CDS             complement(9349..9891)
                     /locus_tag="PK016_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HXL02034.1"
                     /translation="MMRLYIGEVKKSVGRTFQDIEEEDFSSLSWEGVRLPFAQPVRVD
                     ALLTNCGGEILVKGVVDTALVMPCSRCLEPSIQKIKAVFYLEYRNLNRIHRPSDIEAE
                     ARESDEVGYFWEEDNYVEVTQEVEEVILLNLPMKPLCSPDCRGICPICGANRNKEKCS
                     CEEAPDPRLAILKNWAKENL"
     gene            complement(9905..11086)
                     /locus_tag="PK016_02800"
                     /pseudo
     CDS             complement(9905..11086)
                     /locus_tag="PK016_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956840.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
     gene            complement(11105..12100)
                     /gene="pta"
                     /locus_tag="PK016_02805"
     CDS             complement(11105..12100)
                     /gene="pta"
                     /locus_tag="PK016_02805"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HXL02035.1"
                     /translation="MDILSKIRERAKALKKKVALPESQDERVLKAAEIAQKEEVASVV
                     LVGEEEKIAKKAQELNLDLSGVEIVNHLKDEKREFYIDDLYQLRKNKGLSREGAEDLL
                     KSSMYYACMMLREGRVDGVVGGAVLSSPDVIRPALQIIKAAPGIKLASSCFLIVVPDC
                     PYGENGAFLYADSGLNPNPNAEELAHIAITTARTATFLLNAEPRVAMLSFSTKGSAKH
                     ELVDKVIEATNIAHSLNPQLLLDGELQGDAALVPEVARKKAPGSPVAGRANVLIFPDL
                     DAGNISYKLTERLAKATAIGPILQGLAKPVNDLSRGAKAEDIVDQIAVTVLQTQF"
     gene            complement(12184..12687)
                     /gene="coaD"
                     /locus_tag="PK016_02810"
     CDS             complement(12184..12687)
                     /gene="coaD"
                     /locus_tag="PK016_02810"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HXL02036.1"
                     /translation="MLTAIYPGSFDPLTNGHLDIIERGRKIFDHFVVAVLKNPHKQPL
                     FTPEERKEMIEEAVKCFSNVEVEIFDGLLVNFAREKKCQVIIRGLRAISDYEYETQIA
                     VTNRKMAPEIETLFLPTSTEFSYLNSTVVKEIASFGGCIRGLVPPVVEKRLRDKFSKK
                     HSAGNSF"
     gene            complement(12693..13265)
                     /gene="rsmD"
                     /locus_tag="PK016_02815"
     CDS             complement(12693..13265)
                     /gene="rsmD"
                     /locus_tag="PK016_02815"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HXL02037.1"
                     /translation="MGYLYLGGGKDRGRKIVTPSGKQVRPMTGFLRKVIFDIVQEEVP
                     GSVVFDFFAGSGSLGFEALSRGASQVYFLEKDPHLCLLLARNIKTCGFEERARVLKKD
                     FMRSFPFPSRKIKPDIVFVDPPFSLNSRAVLEKLYNFRMVLEDALVVFRYPEEQDPFL
                     DCGFFEIKNRRTYGRSVLVFGSFKLDSQAG"
     gene            complement(13265..15622)
                     /gene="recG"
                     /locus_tag="PK016_02820"
     CDS             complement(13265..15622)
                     /gene="recG"
                     /locus_tag="PK016_02820"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HXL02038.1"
                     /translation="MTKEKINKELVLKIIEREKQRLDNLSVFGGFSNFLSSWFAERGE
                     GEDFSSLLLEYDSLTVEQKRKLLQDMEEKLKSQSDGDGELNSPSPVLPSPEKEKSLST
                     PVRYIKGVGPALEKKLNRLEIKTAEDLLFFFPRYYRDRGEVKPISHLRGEGWETIQGV
                     VSSQSRIATRRGPLLKIAVSDGTGKVNLVCFNRNYLAKTLRKGVKVQVSGNFRRSFGN
                     WEVADFDYQIVDQENSSWDRIVPIYSLTEGLSLKKLQSIIYYALDNFLEEIKDILPSY
                     LVVKHNLLPKRVAIEELHRPSRSSVGELNSRRSKAHLTIIFEEFLLFALSLKIRRQEL
                     AVYDVPPCVSDSALVKNFLAQLPFTLTSAQKRVVKEIHEDLVSGRLMNRLIQGDVGSG
                     KTLVALLAALQVIDSGRQVALMVPTEILAEQHYHRFKDLLSSLGVRVGLLKGGTNKEK
                     KELVEKIKKGEIDLVIGTHALIEENITFPRLGLTIVDEQHRFGVLQRAKLKEKASIPH
                     LLVMTATPIPRTLALTLYGDLDISVVDEKPAGRKPVTTLCFSIKERFKAYNRVRKQIE
                     EGRQAFVVCPAIEESEEEISNVAEVTELLRSSWLSGFRIEMIHGKLPSREKEEIMERF
                     SRGDIDVLVATSVIEVGIDIPNASVMVIENAERFGLAQLHQLRGRIGRGEAPGLCILL
                     AEAHTEEARRRMEIMVATEDGFRIAEEDLRLRGPGEFYGVRQHGVPEFRLADPFEDWS
                     ILEKANEEAKVILDEDPRLEKSENRSLQEEIDRRFGRYLEFGEVS"
     gene            15708..15893
                     /gene="rpmB"
                     /locus_tag="PK016_02825"
     CDS             15708..15893
                     /gene="rpmB"
                     /locus_tag="PK016_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019106947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HXL02039.1"
                     /translation="MARCEICGKTTSFGNKISHSHRVSGRVWHPNIQKVKAVMGGKTR
                     KINVCTSCLKAGKVKKA"
     gene            15907..16596
                     /locus_tag="PK016_02830"
     CDS             15907..16596
                     /locus_tag="PK016_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015415891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HXL02040.1"
                     /translation="MLPLGTLVNAFAILIGGIVGIVIRSRLPERISLIVFQGIGLFTL
                     SLGVSLAIKTQNFLLLIFSIVLGSIIGELLDLEKWLNHLGESLKFLIKSKNDRFAEGL
                     VTAFLLFCMGSMTILGAIEEGLGNPPHLLLAKSVLDGFSSIALSASLGAGVIFSIIPL
                     LLYQGGITLFASSLQNFFNDSLILELTAVGGLLLIGLGINILEIKKLKVLNMLPSLVV
                     VVILKMLFFPN"
     gene            complement(16642..17583)
                     /locus_tag="PK016_02835"
     CDS             complement(16642..17583)
                     /locus_tag="PK016_02835"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HXL02041.1"
                     /translation="MRNCKKKNGTKTSAFGSPGRINHNSTPFYKSRLYEGLPKEEFTK
                     YVENPILPQYLDKVFCKSSEKMEELPDNSVHLMVTSPPYNVGKEYDEDLTLNEYRVFL
                     KRVWGEVKRVLVPGGRACINIANLGRKPYIPLHVFIIEDMLDLGFLMRGEIIWNKASS
                     GSPSTAWGSWLSAKNPTLRDIHEYILVFSKGMFSRESLGRESTIPKEEFLEFTKSVWT
                     FAAEPATKVGHPAPFPVELPYRLIQLYAFEGEVVLDPFMGSGQTAIASIKTKRHYVGY
                     EINEEYVKLAEKRVKEFSLEFNSPRLFEFNGKKDDGY"
     gene            complement(17584..18216)
                     /locus_tag="PK016_02840"
     CDS             complement(17584..18216)
                     /locus_tag="PK016_02840"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010901240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThaI family type II restriction endonuclease"
                     /protein_id="HXL02042.1"
                     /translation="MSDYLIEIFRDEKLVEKIKRRLPYLFQLAELESSRAGKLGMEVG
                     SVREKIIVALLIYKFGEANVETEIPITEPEVDVKLFGEPVSIKTITGKNFSGVKLVWT
                     VDAQKAKEFCENYYPHCDILLVQINWNDIGGFYYIPLEAQKRLFGKIGRENYVKLPKL
                     GTNPRGVEITKEALLSLVGDNSSRSMAINWQRIKIEFNPYRRWVDLWKED"
     gene            complement(18391..19644)
                     /locus_tag="PK016_02845"
     CDS             complement(18391..19644)
                     /locus_tag="PK016_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXL02043.1"
                     /translation="MKKIIEQTVAEYYLKSNDFNGIQIWNLLSVVGKEWTEIKPLIQD
                     LIKSDLIRIIDGKTDINPNIIRIGFEPLEVQLEKANADEPGYMCLYPGKLILKKMVNP
                     DDYKSEPYKLRLALGEPQLSYVSFDLSVLEFYRNDPRYRYENDDIQGRIHYNNEQMPE
                     SDKAFLETYGFAYDESLNRAVAVFLRYLADLTPEHQQIWKAKELPDSFKLHPAYYRNT
                     IIGDWGEGVPICQALLMELFIINQMSEAMERPPLFRNDYGKDRPKKFHFLIRPTLEEF
                     NNFVLLFDKMLSDNINKDFFMNDICYEEEQRRKDGKIIVTQKGTLQLLDEWIRKFFRP
                     ADWLIWNEAITSLKEVRKLRQKPAHVIEEDIFDQKYFKDQRDLILNAYSAIRTIRLIF
                     ANHPKVKKASIDIPDCLREGKIWDI"
     gene            complement(19811..21199)
                     /locus_tag="PK016_02850"
     CDS             complement(19811..21199)
                     /locus_tag="PK016_02850"
                     /inference="COORDINATES: protein motif:HMM:NF037531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02044.1"
                     /translation="MIKEFEYKGIWWLPDNPEKRISGTLRFTPNEGAILDLIGSIEDI
                     KNMNKTLEPEIVLGISSNGKNITLYKCFETKSSFSSEGFQTSLFYANFVFIGVHFQKS
                     EDIRFKSISVHCLHLDEWVNISGFDIKPLGEEKEIIIKYKLPESFQADIGDGLKILIN
                     IQVTGPSLAFVQKEAAIKQETEIEIETSEDKSFEDCIKVIRQIQNFLTLGITEPAYPL
                     AITGLTEVNKEMVEGKICYPPVEIFYRLPDISKASKTLLPVDMLFTFKDISNRFESFL
                     RTWFGEKDSLEPVYDLYFGTLYNPRMYLEHHFLSLIQAIESFHQCTCGGEYLSDEGYK
                     AVYKALVTAIPENVGSDLKDRLKEYLKYGNEFSLRKRLKEFFDKYRKILDKFIEDEDA
                     FIEKVVNTRNYQTHHDKELKERAVSGEDLYHLTQKLKMCLEVCLLAELGFSLEEIKDL
                     FSRNSRYRQESI"
     gene            complement(21308..21937)
                     /locus_tag="PK016_02855"
     CDS             complement(21308..21937)
                     /locus_tag="PK016_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02045.1"
                     /translation="MGIFNIFKKESKQIQGKIGYFGLGDWWLSNFSEEEREHIIKTFQ
                     PFGSSGESLIKGNIASTSETPIGLLSALASWFSKKDDRTIAYRIIKKAEEMIDESSNI
                     LDIHFLYQSKIEIYYRNRDNDPDALEKAIEACKQQIEISPKAKAAFQREYKDSPLPAH
                     KGFGQLAIIEEKRKNFEAAIQISKAALEQGWAGDWEKRIERCSRKLDKK"
     gene            22861..23139
                     /locus_tag="PK016_02860"
     CDS             22861..23139
                     /locus_tag="PK016_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HXL02046.1"
                     /translation="MPRVTFKLDYPQAQSVFIAGGFNNWDTQAYPMRKTQKGHWSCTL
                     TLPCGRYEYRYIVDGEWYTDPSTPRVPNEYGSENSVIEVKGRETKKGG"
     gene            23162..23434
                     /locus_tag="PK016_02865"
     CDS             23162..23434
                     /locus_tag="PK016_02865"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HXL02047.1"
                     /translation="MCKVIFEVYAPRAKSVYLVGDFNEWDIRACPMKKKNGGWAASLD
                     LPPGKYEYRYLIDGGWYNDPQTYRVFTDWGENSILEVEEDCSSISV"
BASE COUNT         6550 a         5744 c         3750 g         7514 t
ORIGIN      
        1 gtcattcctg agtgtattta tcaggaatcc agaactataa gggggtaaac ccccaaaaga
       61 caaaagaaca taaagaaaaa acttactgag gaaggaaaag catcttcctc agtggattcc
      121 cgattgaaga tttcgggaat gacgagtaat gaagattccc ggctaaatct cgggaaggac
      181 aaaaactaaa ttcttgactc ttcttccccc ctccttatcc accctaccaa atcattgatt
      241 tggtagggga cccgggcact cccttccggg aggagaaaaa ggcaaaagac cactggagaa
      301 aatatagtat cccttactgc acggcaattt ccccattttt tgacttccga tgtaatttgc
      361 ttttaaaagt ttaatgttta acaggtttta tggaatacta tatttaataa actatagttt
      421 ttacaaattc ttccgcatca aaggaaacta aatcctctat cccttctcct acccctaaat
      481 acttcactgg aagatgaaac tctttaccaa tgcggaaaac aattcctcct ttagcctggc
      541 tatcccattt agtgagaatg ataccactga ggtcagctac cggaaataga gattgagcct
      601 gtaaaaaagc attctgccca gctaaagcat caataactaa aagactctct aaattagaag
      661 gaagaattaa ttttttagtc actcgcacta ctttttccag ctcggcaagg agattcctat
      721 taacatgaga cctccccgcc gtatcaatga taactaaagt gtctccctgg tggcgagcag
      781 aattgattcc gtcaaagacc accgctcctg gatctgcgcc ttctgtccct cgaaaatagt
      841 ggcatttcgc tctttgagcc caaatctcta attgttccat agctgcagcc cgaaaagtat
      901 cagcggcgat gaggttaact ttctcccctt tatttttaaa gagataagcc agcttaccag
      961 ctacggtggt cttccccact ccattaactc cagaaagaat tatagctttg agctccccct
     1021 cttcagcgga acgaaaaaga gaagggtcat ttccctgtaa aagagagaac agtttttgat
     1081 acaaccactc ttgccagtcg ccttccctct gagatttttt gaattcccga aaatcttcca
     1141 ctatttccaa agatagtgca ggacccatat cggcctgaat aagcaattct tccagctctt
     1201 cccagtcttt ttctcctact tcttttttgc tccataactg ggaaaaatgt tcctttacct
     1261 cctccttcat ttttcccata ccttttaccc atcgctgcca gatagtttct ttctccatca
     1321 ttttctcctt ttctattgaa aggactcccc gtcaaaacaa actgcctggg aaactcctgg
     1381 ttcattcata gtcattccaa ttattcgatc tgctacttcc atcacttctt cctggtgagt
     1441 taccaccacc acctgccggt tgtgagaaat attcttcagt agctgagcca gaaggaggct
     1501 gttagcgtgg tctaaattgg catccacctc atccagaaag caaaaaggta aattagctgt
     1561 ttccagaacc gcaaaaagaa aacacaatgc caccagagat ttttccccac tggaaagaag
     1621 agataaaatt tgttttctct ttcccggtag acgtacttct atttctgctt tctcttcttg
     1681 aaaaaggaga taagcttctc ctccaggaaa aatctcttta aaaaagccag aaaaactttt
     1741 attcacctga taaagaaact cctgaaaact ttttctgatt tctctccggg ccagagaaac
     1801 cccctccctc accaaagaga ggcccgcttc acagcattca tttttttcct ttaaggaaga
     1861 aaagcgctcc tctacccgaa caaactcttc acctgctcca ggaataaact tctttttccc
     1921 caacaattct tctttttcct ggagagaacg ttccagctct tttatatcta tacagattga
     1981 ggaaggtgca gggcgaaaat tccccatttt ttctaattct tgcgagagtt cttttacctc
     2041 taaatctaac ttttcttcta aaaaaagagc tttatctaat tttttccttc tctcctcttt
     2101 ttccttttct atctcctgaa tttttttctc agccagacta atctttccat ctaaactctt
     2161 ttcctcttcc tctttttgtt tttgttcttt catcttctct gctattgaaa cctgtatcac
     2221 ctggatttta cttccctctt cactcaatct ttttccatac ttcctttttt cctcttcttc
     2281 tacttttaga acttcaagag cttctcctat ttctttcttc agagattgta tttcccacag
     2341 gaacctctct ttttcccttt ctctctcttc tttctttctt ttatctttct ctctttcttt
     2401 tatactacac tggatttgag aaatattttc ttttatcctt actatttctt cttctgtgac
     2461 acttaattct ttttcctcag catttttttt accggctacc aactctatct ctttttccag
     2521 ttctcttatc tttctttctc tctcctcttt ctccttttcc cggtaaacta atttctcttt
     2581 ctctctttct atttccagct ttctcttcca caacttctct tcttcattct tctcttcttt
     2641 agaaatatct cgaatcctac tttcccagaa agctactttg tcctgccact tcttctccct
     2701 tttcttccag gaatggtaaa aatgagactc tcctacagaa gaaattaatt gtccaggaaa
     2761 caaaagaaat ccactgcgca gaaagacgag atttccatcg gaggatacta gattctcctc
     2821 cggaagttta ccactctgga aaagatgaaa cacctccctt ttctgacact ctctccagag
     2881 agcaggaggt atagcagaag aagaaatata ccattgcttt gatcctggca aatctggctc
     2941 tgaatcaaaa ccttcttctt taatattctg gctctcccag aaccagttaa gagcctgtac
     3001 tgctcgtcga gaccagccca gaacacttaa ttctttctcc cactcctcac ttccttgctt
     3061 tctctgcttc aagttattta gcttccggga aatactttgc agaatcactt tacctttttc
     3121 taatttttcc cttaaaattt tttcctcctc ttttcccctc tctattttat tttctacttc
     3181 tccaatgttt tcctctccct tttttatctt agtttttagc tccagaatct cctctcctat
     3241 tcttttctct ctgcgtatct catcctggag ggaagcttcc aactttttct tatcctcctc
     3301 tccgtggcgt ttacattcct gcaaataaat tttctttttt tctaactccc ggagagtgtt
     3361 tgctctttct tcaagctgga gcatcaattc ttcgtcatct atttcctcta ccttatcctc
     3421 cggaaaagaa aaatcctctc tccacttctt ttctctttct tcccagagat taaccgttct
     3481 ttcccaggaa gaaaattttt ccttaattcg caaaatgttt tttctatttt ccctcatctc
     3541 agttaaaaga tagtagagtt tttcttcttc cttctttttc tccctttcct tctctcctct
     3601 ttctctttct atatctttct tttctctctg gcatttatcc cggacaatag aaagagaaga
     3661 aaaaatatcc ttccactttc tttcctcttt ctctccttcg gctatttttt cttcttctgt
     3721 ctctatttct ttcttaagga gagaattttt ctcttcatgg agccttatca tctcctgaat
     3781 ataaagtgct ttagtatctc tccattctct ctctaaggag agatactctt cttcctcttg
     3841 agcccataat ttccattttt ccaattgaat atggagctcc ttcaatcgct cctccagaag
     3901 ctttcttcta ctctcaagtt tctctatgcg gagagaaact tgagagagct tttttttcca
     3961 ttccccagca ccgcttatca tctctaacca ttgccaccgc tccagtggtg tctcataaag
     4021 cagagattgt agctcccctg cacccactac accagcgctt aaacgctcta ttcccaaacc
     4081 ttttttttct aatttctctt tccaatctct aaagcggatt ttttcatcac gaaagaaata
     4141 ttctgactct cctgaggcaa atattctcct tgctactttt agatctgcac ctgaggagtc
     4201 ttcccaccac acttcaatat cagccattcc cagaggttta gctgtcctgg acccatggaa
     4261 tataacctcc cggttttgag taaccctgag ctcccgtgct gtatctcccc atacccagcg
     4321 cagagcatca atgagattac tcttacctga accattagga cccacaacca cgtttaaacc
     4381 tggagaaagt tcaatagcgg tgaaatgggc aaaagatttg aagccttgaa tgattatttt
     4441 ctttacatac attattgacc tttattgaga agttgattat cactgcttaa aacttctaaa
     4501 gctttcttag ctgcttcctg ttctgctctt tttttattct ttccctctcc ccgggctata
     4561 ctattttctc caatttttac ctctatctga taaaggttct cttccccctg atttaaaatc
     4621 tggtaatgag gaagacaatt ataatattgc aaacaccatc gctgaagcaa ccctttataa
     4681 ttcactgaca ggttaagaga atcaatgtct cctgcctcgc aaaaaattgt tttcaaaact
     4741 tttttagttt cctcataaga agaagaatca acaaaaactg ccccggccag cgcttctaag
     4801 gagctggcca gtatagaatc tttttcccgt cctccacttc tttcttctcc tttacctaac
     4861 tcaataaact ttgccagttc aatcttccgg gctaaataag ccaaacactc ttcgctcacc
     4921 cattttgatt tagttaaaga taaccagctc cggggtttat cataatggcg aaagaggtag
     4981 tcggtgacaa aaaaaccgac taccgcatct cccaaccatt ctaacttatc attacagcta
     5041 ccatcttttc cttccagtgc tgatttatgg cgtagggcaa ttttgagcaa ctcctcattc
     5101 ctgaaaacat acctgattct ttcttccagc actgaaaagt caatgtctga actaaacacg
     5161 gggaaacaaa ctactctatt ttagattcaa tgtattctat cgcttccccc actgtggtta
     5221 ttttttctgc atcttcatcc ggtatttcaa tatcaaattc ttcttcaaaa gccataatta
     5281 actccacaat atccagagaa tcagccccta aatcatcgat aaaagaagca tcgagagtaa
     5341 catcttcttc atctactcct aactgttcca ccactatttc tttaacttta gaaaaaatat
     5401 ccatagttac acctcctacc tgaaatagtt tttaaatcat caccaatccg ccattgacat
     5461 ggatggtttg accagtaata tatccagcag ctcgagaagc cagaaaagca accgcttgag
     5521 ccacctcttc aggttctcca aagcgacgta gaggcacctg ctgaagccag acttctctca
     5581 cctcctccgg cagtttctca gtcatttctg tatctataaa accaggagct acggcgttaa
     5641 ccgttatacc taaaggtgct acctcccggg ctaaagattt agtaaaacca agaatagccg
     5701 ccttcgcagt agaatagttg gtttgaccag cattacctac tactcctacc actgaagaaa
     5761 tattgattat ccttccatat ctctttttca tcatataccg caagacttct ttggtacaat
     5821 aataaacccc ctgcactcct acatcaagta ctgctcgcca gtcctcatct ttcattcgca
     5881 cgaggagcgc atctcttgta atcccagcat tattaactaa tatatcaacc tgcccccagg
     5941 tctttattat atcctgcact acagttttaa tctcttcctt tttggtaaca tccgcgacat
     6001 atccccgggc ttctatccct tgctccttta gctctgtcac tgccttttct atttcttcct
     6061 gaggagccac atcatttata gccacccggg caccacccct acctaaaact tgagcaatac
     6121 taaaaccaat gcctcgtcta cccccggtca ccatggctac ctgattttcc aggctcattt
     6181 ttctttttcc tccttattct cttttctccc cccatcccag gaatttacat tttttacttc
     6241 cacttcagga tattcccggg aaaaaagttt actcaatact gttccagggc ccatctccca
     6301 gacctggtga gactcaccac gatagagaac atccataact tccttccatc gaaccggaga
     6361 aaacatttgc ttaaccagta aatccctgat ttcttcaggc cgggaaacat attgagcatt
     6421 cacattactt acatatcggt aacggggagg cttaatttct atttcctgca aaagagaaga
     6481 aaatttctct gccgcttctt tcataaaact ggaatgaaaa ggagcactga ctttcaattc
     6541 caccactttt ttactcccct cttttttaag tctatccacc acttgaggta aaagagaacg
     6601 ggaaagagaa agaactacct ctgaggaaga attaatatta gctatttcta ctttatcccc
     6661 cgaggaaaac tcttctacca cattctccac tttttcttgc ggcagcccta aaactgctac
     6721 cattgctccc tccccgggag gaatggcttc ttgcataagc tctcctcttt ttcgcaccag
     6781 aaaaattgcc tcttcaaagt ctatgctccc tgcggcacaa agggccgaat attcccctaa
     6841 actgtgtccc gccactgcat ccacgggaag agaaaattct ctctgccaga ttcgccagat
     6901 gagtaaactg gtagctaaaa tagctggttg agcattaaaa gtgaggctca gttcttcctc
     6961 cggcccttcc agagttaagt gcaacaggtc tactccacaa atttggctcg ctttccccag
     7021 aatttcctcg gtctcctggc gataattctc ccaccaaatt ttagtcatac ctgctttctg
     7081 tgccccctgt ccggggaaaa gaactaccca gcccatttta atcatccacc atctgaggca
     7141 attttttaac cgtctcctta aactctgcaa ttatatcttc aatgacctgt cgaacaggtt
     7201 taatatcttt tataaggccg gcaacttgcc ctgacattac cgaaccttct tctacattac
     7261 cacaaactgc ctctctcagt ctaccggagc ccagagcttc tatttctgca agaggagctc
     7321 tttgagcttc cagctcctca aacttgcgcg tgagacgatt acgcagacag cgcaccggat
     7381 gaccagttgt attaccggta accacggtag aacgatcttg agcttttaca atcgcttctt
     7441 tgtaaagagg atgcacctca cattcttcag tgcaaacaaa acgagtgccc atctgtattc
     7501 cttctgctcc taaagcaaaa caagctgcca tacctcttcc atcggctatc cctcccgcag
     7561 cgatcacggg aagaggacta acagcatcca ctatttgagg aaccaaacac atggtggtta
     7621 tctctccaat atgaccaccg gcttccattc cttcagcaat aattaatttt attccccgac
     7681 gcaatagcct tttagccaaa gcaacagatg ctaccacagg aatggttaag atacctctcc
     7741 gggcaaaatc ctccactagc tctcctggat ttcctgcgcc ggtagtaacc acgggaactt
     7801 tttcttcttt aaccacttcg atttgctctt ttacataagt agataaaagc atcaagttta
     7861 ctccaaaagg ttgtccggta agagatgtag ttcttcgtat ctcctctcgc agaccctctc
     7921 ccgtcatccc ccctgccccg attataccta aacctccggc ttctgaaacc gccgccacca
     7981 aaggtgcagt agatacccag gccattcccc cctggaggat gggatacttg atacctaata
     8041 tttctgtaac tcttcctcgc ataatccacc cctaactttt caaattaagc tgggacaaac
     8101 gctcacgaat tttatctact attccttttt ctaccagctc tttagccccc ttaatggcgc
     8161 tttttatgtc tcttgctttt gagctaccgt ggcaaattaa gcatactcct tctattccca
     8221 aaagaggagc tcctccatat tcacttctat ctaatttacg ccagaattct ttcagcttct
     8281 cttcctcctg aggactacct atttcttgag gaaggtgaga agaaataaga gcaattaaac
     8341 cttctccaaa ttttaaaaga gcattgccag taaaaccatc acataccact acatctgctt
     8401 ttccatccac tatattgtag ccttccacat taccagcaaa gtcaaagtgc aaaaaatcac
     8461 tttgagagcg aaacagctga taagtagatt tcacgagttc gtttccttta ccttcttctt
     8521 ctccaatatt caataacccc acccgggggt gaggcagatt caaaactatc ttagcatact
     8581 ctgcccccat aaccgcaaaa tgaagaagat gtttgggttt acaatctacg ttggctccca
     8641 catccagaag aacggtgcag gaattagaat tgggtataag agcagtaata gctggtcgct
     8701 ctatctggga aacccgcttt agcttcatcc aggcagtagc cataactgct ccgctattcc
     8761 ctgctgatat gaaggcgtct attttcccct gagccaataa atcaataccc ttagctatag
     8821 tcgaatcggg ttttttctta agagcttcgg taggtgattc tcccatgcct actatttgag
     8881 aagcgtcttc aatgggaaaa tgttcttcgt ccattcccca ttgagaatag agagatagga
     8941 gtttctgtcg agggcctacc actaccaggg tataatcagg agaataggct tccctcactc
     9001 cttttattat ttcctcggga gcgaaatctc ctccccaccc atccacagca atcctcatat
     9061 tcagcctctt ttagtcttta accttcacca cttgcttacc tttatagtaa ccacaagccg
     9121 gacagacccg atgaggcaac ttaggctgat ggcagcgagg acaatctacc agctgaggag
     9181 ctttcaccac ccagtgagtt cttctcttat tacgtcgaca acgagaagtc ttattagtta
     9241 aattagccat attcttataa tacctcctct tttgctctcc atcctgaatg taggatagta
     9301 taaaataatt tcctgcctat tgcaaatgaa attacttcat tccccgaatt ataagttttc
     9361 tttagcccaa tttttcagta ttgccagtcg agggtcaggc gcttcttcac aggaacactt
     9421 ttctttatta cgatttgctc cacaaatcgg acatatcccc cgacaatcag gagaacacaa
     9481 aggcttcata ggtagattca gaagaataac ttcttctacc tcctgtgtta cctctacata
     9541 attatcttct tcccagaagt accctacttc atcagattcc ctggcctctg cttctatatc
     9601 agaaggacga tggatacgat ttaagttccg gtattccaag taaaaaacag cttttatttt
     9661 ctgtatcgaa ggttctaaac agcgggaaca gggcatcacg agtgccgtat ccactactcc
     9721 ttttaccagg atttcacctc cacagttggt aaggagagca tctaccctca ccggttgagc
     9781 aaaaggtaac cttactcctt cccagcttaa agaggaaaaa tcctcttctt caatatcctg
     9841 aaaagtcctc cccacacttt ttttcacttc cccgatgtaa agccgcatca tcctcacctc
     9901 tttctcttgc cagaacccag gtatctcggg cgattatcag ttcttcgtta gtaggaataa
     9961 ccattactgc tacccgggac tggtcgctac ttataaaagc ctcttttctc ctcactttat
    10021 tccgctcatc atctatcaat atacctaagt gctctaatcc ctgacaaacc gactttctaa
    10081 tataggagga attctcccct ataccagcag taaaaactaa accatccagt cctcctaaaa
    10141 tagcgtaata agagccaata tattttttag cccggtaagc tatcaaatcc agagttagct
    10201 gagcccgatg attaaaagga gcagcatctt cgatatcccg agtatcgctg ctaactcctg
    10261 agacacccag cactccactt tttttattca atatatcatc tatttcatca actgccagat
    10321 tttcttttct catcaaaaat agaggtatag ccggatctaa atctcccgag cgcgttccca
    10381 tcaccaaccc ctctaaggga gtaagcccca tagaagtatc tatagattta cctcccttta
    10441 tggctgcaaa gctcaccccg ctacccatat gacaggtaat taattttaaa tcgccaaggt
    10501 cttttcctaa cattttagcc gctctctcgg acacatatcg atgagaagtt ccgtgaaaac
    10561 cgtatcttct gacccggtat ttttcataat actcataagg aatagcataa atataagcat
    10621 attcgggcat agtgccatga aaagcagtat caaaaactgc cacctggggc acatcgggca
    10681 ttaactcctg gcaagcttct attcctaaaa tattggcagg gttgtgtaag gggctcaatt
    10741 cagcacattc cttgataatc tttaaaactt catcatcaat caacactgaa ccagtgagtt
    10801 tttctcctcc atgcaccact cgatgaccta tggcttcaat tctattagtc tgagtttgtt
    10861 cccgggttaa aacttccatt acccactgca tagctacccg atggttagaa acttctcttt
    10921 cttctacaaa actatggtta tcatttttgt gctctaaccg agaacccggt gttccaattc
    10981 tctctaccat accttttgct aatacctggt aatcttcctc cccttccata ccaaacagtt
    11041 gatatttaat cgtcgagctt ccacagttga ccaccagtat attcaatttt ctatcctcct
    11101 aaaattaaaa ttgagtttgt aaaacagtaa ctgctatctg atctactata tcctctgcct
    11161 tagctcctcg agaaaggtca ttgaccggtt tagcgagacc ttgcaaaata ggaccaatgg
    11221 ccgtagcttt agctaagcgc tcggttagtt tgtaagaaat attaccagca tccaggtcag
    11281 gaaaaattaa aacattagct ctcccggcca ccgggcttcc cggcgccttt tttctggcca
    11341 cttcaggaac caaagccgca tcaccctgta actccccatc taaaagcaat tgaggattca
    11401 aagaatgagc aatattagtt gcttctataa ctttgtccac tagctcatgt ttggcgctac
    11461 ctttagtaga aaaagaaagc atagccactc gaggttcggc attcaaaaga aaagtagccg
    11521 tgcgtgcagt agtaatggca atatgagcta attcctcagc attggggttg gggtttaacc
    11581 ctgaatcagc atacagaaaa gcaccattct ccccgtaagg gcaatcggga accactatca
    11641 aaaagcaact ggaagctaat ttaattcccg gtgccgcttt aataatctgc agagctggtc
    11701 ttataacatc aggactggac agaactgctc ctcctactac cccatctact ctaccttccc
    11761 gcaacatcat acaggcatag tacatagaac ttttcagtaa gtcttctgct ccctctcgag
    11821 aaagaccctt atttttcctc aactggtaaa ggtcgtcgat ataaaattcc cttttctcat
    11881 cctttaagtg gttgactatc tctacccctg aaaggtcaag attgagctcc tgtgctttct
    11941 tagcaatttt ttcttcttct cctacaagaa ctacagaagc tacttcttct ttttgggcta
    12001 tttctgctgc ttttaaaact cgctcatctt ggctttcagg taaagcaact tttttcttca
    12061 gcgctttggc tctttccctg attttactta atatatccat tctatctacc cctttttata
    12121 atattaaaat ctcttttatt accccactac caaaaatctt agtccaaaaa gggaatatct
    12181 tattcagaaa gaattccccg cggagtgttt cttgctaaat ttatctctca gcctcttttc
    12241 tactacagga ggaactagcc cccggataca accaccaaaa gaagcaattt cttttactac
    12301 agtagaatta agataggaaa actcagtgct ggtgggcaaa aataaagttt ctatttctgg
    12361 ggccatcttt cgattagtaa cagcaatttg agtttcatat tcgtaatcag aaatagctct
    12421 caaacctcta atgattacct gacacttctt ttcccgagca aagtttacca gtaagccatc
    12481 gaaaatttcc acctctacat tggaaaaaca ttttactgct tcctcaatca tttcttttct
    12541 ctcttcggga gtaaagagag gttgcttgtg aggattcttt aatactgcca ctacaaaatg
    12601 gtcaaaaatt tttcttcctc gctcaatgat atccagatgt ccattagtta aggggtcaaa
    12661 gctccccgga tatatagctg ttagcaccgc cttcaccctg cctgagaatc aagtttaaaa
    12721 gagccaaata ctaaaacact tcttccatat gttctccgat tttttatttc aaaaaaacca
    12781 caatccagaa aagggtcttg ctcttcaggg taacgaaaca ccaccaaagc atcttcaaga
    12841 accatcctaa aattatacaa cttttctaaa acagctctac tatttaaaga aaagggagga
    12901 tctacaaaaa cgatatccgg cttaattttt cgagaaggga aaggaaaaga gcgcataaaa
    12961 tcctttttta aaacccgagc tctctcttca aagccacagg tttttatatt tctggcaaga
    13021 agcagacaga ggtgagggtc tttctccaga aaataaacct gacttgcccc ccggcttaaa
    13081 gcttcaaaac ccagagaacc actcccggca aaaaagtcaa aaaccacact ccccggtact
    13141 tcttcttgca ctatgtcaaa tattacttta cgtaaaaaac cggtcatagg acgaacttgt
    13201 ttccccgaag gggtaactat tttccttcct ctatcttttc ctcccccgag atataaatag
    13261 cccattagct tacctctcca aattccagat aacggccaaa ccgccggtca atttcctctt
    13321 gaagagaacg attttcactt ttctccagcc ggggatcttc atcaaggata acttttgcct
    13381 cctcattagc tttctcgaga atagaccaat cctcaaacgg gtcagctaaa cggaactcag
    13441 gcactccgtg ttgtctcact ccataaaact cacccggccc ccggagacga aggtcttctt
    13501 cggcaatgcg aaaaccatct tcagtagcca ccattatctc catccggcgg cgagcttctt
    13561 cagtatgagc ttcagccaaa agaatacaga gacctggtgc ttctcctctt ccaattctac
    13621 ctctcaattg gtgcagttgt gccaaaccaa acctctcagc attttctatc accataacac
    13681 tagcattggg gatgtcaatc cccacctcga ttactgaggt agccactaaa acgtcgatat
    13741 ccccccggga aaagcgctcc atgatttctt ctttctcccg agaagggagc ttaccatgaa
    13801 tcatctctat ccgaaagcct gaaagccagg aagaacgcaa aagttctgtc acttccgcta
    13861 cattactgat ttcttcctca ctttcttcta tagccgggca caccacaaag gcctgtctcc
    13921 cttcctctat ctgctttcgc actcgattat aagctttaaa tctttcttta atggaaaagc
    13981 agagagtggt tactggtttt ctacccgccg gtttttcatc caccacggaa atatctaaat
    14041 ccccgtaaag agtcaaagcc aacgttcggg gaataggagt ggcagtcata accaggagat
    14101 gcggaataga agctttttct tttaattttg ccctttgcaa tactccaaag cgatgttgtt
    14161 catctacaat agtaagaccc agccggggaa aagtaatgtt ttcctcaata agagcatgag
    14221 tgccaattac gagatctatt tctccttttt taattttttc caccaattct ttcttttcct
    14281 tattagttcc ccccttaaga agccccactc tcactcctaa agaggataac aaatctttaa
    14341 accgatggta atgctgttcg gctaaaatct cagtaggaac catcaaagct acctgtcttc
    14401 ccgaatcaat aacctgaaga gccgcaagta aggctaccag cgttttccct gaacccacat
    14461 ctccctgtat taaacgattc atcaatcgtc cactcactaa atcctcatga atttccttta
    14521 ccaccctctt ttgagcggaa gtcaaagtaa aaggaagttg agctaaaaaa ttttttacca
    14581 gagctgaatc agaaacgcag ggaggaacat cgtaaactgc taattcctgc cggcggattt
    14641 tcaagctcaa agcaaaaagt aagaattcct caaaaatgat agttaagtga gctttgctac
    14701 gtcgagagtt taactcccct actgaagaac gggaaggacg atgcagctct tctatagcca
    14761 ctcttttagg taaaagatta tgctttacca ctaaataaga aggaagaatg tcttttattt
    14821 cttctaaaaa attatccaaa gcataataaa taatggattg cagttttttt aagcttagac
    14881 cttcagtaag gctgtagata gggactattc tatcccagct ggaattttcc tggtccacta
    14941 tttgatagtc aaaatcagct acctcccagt taccaaaaga ccgacgaaaa tttcccgaaa
    15001 cctgaacttt cacacctttt cgcaaagtct tagccagata gtttctatta aagcacacta
    15061 gattcacttt acctgttcca tcactcaccg caattttaag tagcggcccc cgtcgagtgg
    15121 caatccgact ttgagagcta actactccct ggatagtttc ccatccttct ccccggagat
    15181 gagaaatagg ttttacttcc cctcggtccc gataataacg agggaagaaa aagagaaggt
    15241 cttctgccgt tttaatttcc agacgattta attttttctc cagagctggc cctactcctt
    15301 taatgtatct taccggggta gagaggctct tttccttctc cggggaaggt aaaactggag
    15361 aaggactatt aagttcacca tcgccatcac tttgagattt cagcttttcc tccatatcct
    15421 gcaacagttt tctcttctgt tctacggtta agctatcgta ctcgagaaga agggaagaaa
    15481 aatcttctcc ctctccccgt tcagcaaacc agctactcaa aaagttactg aaccctccaa
    15541 aaacggagag attatccagc ctctgttttt ctctctctat tattttcaat actaattcct
    15601 tatttatttt ttccttagtc attttttcta ttttaaccca tcttgcatta catcttcaaa
    15661 actgctatat ttttacccga ttctgaaaaa ggaaaggagc aaagacaatg gcaagatgcg
    15721 aaatctgtgg caaaactact tcctttggta ataaaataag tcactctcac cgggtaagcg
    15781 gaagagtatg gcatcccaac attcagaaag taaaagcagt catgggtggt aaaaccagaa
    15841 aaataaatgt ttgcacctcc tgtttgaaag caggaaaagt aaaaaaagcc tgaattacca
    15901 ccttgtatgc tacccttagg aaccctggtc aatgcttttg ccatccttat aggaggaata
    15961 gtagggatag tcatccgttc ccggctacca gagcggatat cacttattgt tttccagggt
    16021 attggtttat ttaccctctc tctgggggta tctttagcca tcaaaaccca gaatttttta
    16081 ctcttaatat tcagtatcgt cctgggttcc ataataggag agctgctcga tttagaaaaa
    16141 tggctcaacc atttaggaga aagccttaag ttccttatca aatcgaaaaa cgaccgtttt
    16201 gctgaaggtt tggtaaccgc ttttttactt ttttgcatgg gttctatgac cattttagga
    16261 gctatagagg aaggtttagg taatcctccc catctacttt tagctaaatc ggtattagat
    16321 ggtttctcct ccattgctct atctgcctct ttaggagcgg gagtgatttt ttccatcatt
    16381 cctcttcttc tctaccaggg tggcataacc ttgtttgcca gttctctcca gaatttcttt
    16441 aacgattctc tgatactgga gctgaccgca gtaggaggtt tgctcctcat aggtttagga
    16501 atcaacattt tagaaataaa aaagctaaaa gtgctcaata tgctacccag cctcgttgtg
    16561 gttgtaattt taaaaatgtt attctttcct aattaaacca caaaactaac cccagttgca
    16621 cccccagaaa aaagagcaac ttcagtatcc atcatctttt ttgccattaa attcaaatag
    16681 cctgggagaa ttaaactcta aggaaaattc tttaactcgt ttttctgcca gttttacata
    16741 ttcttcattt atttcatatc caacgtaatg acgctttgtt tttatcgatg caattgcagt
    16801 ctgcccacta cccataaatg gatccagaac aacctcaccc tcaaaagcat aaagctggat
    16861 cagcctgtaa ggcaattcca ctgggaatgg tgcaggatga ccaacttttg ttgctggttc
    16921 tgcagcaaat gtccacacac ttttagtaaa ttcaagaaac tcctccttag gaattgtact
    16981 ttcccttccg agactttccc ttgaaaacat ccctttagaa aagaccaaaa tatattcatg
    17041 aatgtctctc aatgtcggat ttttagcaga aagccagcta ccccacgcag ttgaagggct
    17101 accacttgag gctttattcc aaatgatttc acctcgcatt aaaaatccta aatcaagcat
    17161 gtcttcaatg ataaatacat gaagggggat gtacggtttc ctccccagat tggcgatatt
    17221 aatacatgcc cttccgccgg gaacaagaac tcttttaact tcgccccata ctctcttcaa
    17281 aaatactcta tactcattta aagtaaggtc ttcatcatat tcttttccta cgttataagg
    17341 cggcgatgta accattagat gaacactgtt atccggtagc tcttccatct tttcgcttga
    17401 cttgcaaaaa actttatcca ggtattgagg tagaattggg ttttctacgt attttgtaaa
    17461 ttcttctttg ggcaacccct catatagcct actcttataa aaaggagtag aattgtgatt
    17521 gattcgtcca ggagagccaa atgcgcttgt tttagtcccg tttttcttct tacagtttct
    17581 catttagtcc tccttccaga gatcaaccca tcttctatag gggttaaact caatctttat
    17641 tctttgccaa tttattgcca tgcttctgct gctattatct ccaactaaac ttaggagagc
    17701 ttctttagta atttcaacgc ctcttggatt tgttccaagt tttggaagtt taacatagtt
    17761 ctctctacct attttaccaa aaagcctctt ttgagcttct aaaggaatat aataaaatcc
    17821 tcctatatcg ttccagttga tttgaacaag caaaatatca caatgagggt aataattttc
    17881 acaaaattcc tttgcctttt gggcatcgac agtccatacc agtttgacac cactaaaatt
    17941 tttacctgta atcgttttaa tcgatactgg ctcaccaaat aatttcacgt ccacctcggg
    18001 ttcagttata ggaatctcag tttcaacatt tgcctcacca aatttataaa taagcaaagc
    18061 cacaattatt ttttctcgaa ctgaaccaac ttccattcca agtttacccg ctcttgaact
    18121 ctctaactct gcgagctgga acaagtaagg taaacgtctt ttaattttct ctaccagttt
    18181 ttcgtctcta aatatttcta tcaggtagtc agacattatt tactcctcta ttctttcgta
    18241 agcaaaatga cgcataacga tttcggtgta tgcgaatttg agaagcgaag tggagtaatt
    18301 tgagcatagg agcagatgcg tcaaagatag gtctacgctg ttgtgtgctg taccttgagc
    18361 aaagactctt tgccggtagt gcatcctttt ctaaatatcc catatttttc cctcccttaa
    18421 gcaatcagga atatctatgc tggctttctt tacttttgga tggttagcaa atatcaacct
    18481 aatagtacgg atagcactat aggcattcag tatcagatcc cgctgatctt taaaatattt
    18541 ttggtcgaag atatcttctt cgattacatg tgcaggcttt tgcctcaatt ttcgaacttc
    18601 ttttagggat gtgattgctt cattccatat taaccagtcc gctggtctaa agaatttacg
    18661 tatccattcg tcaagaagtt gaagagttcc tttttgcgta acaattattt taccatcttt
    18721 tcttctttgt tcctcctcat aacaaatatc attcataaaa aagtctttat ttatgttatc
    18781 agatagcatt ttatcaaaaa gcagtacaaa attattaaat tcttctaatg ttggcctaat
    18841 tagaaaatgg aatttcttcg gtctatcttt tccataatcg tttctaaaga gaggaggtcg
    18901 ttccattgct tcggacattt gattaataat aaagagttcc attaataggg cttggcagat
    18961 aggaacgcct tctccccaat caccgataat agtattccga taataagcag gatgtaattt
    19021 gaaactgtca ggcaactctt ttgctttcca aatttgttga tgttcaggtg ttaagtcggc
    19081 aaggtacctc aagaagacag caactgccct gtttaggctt tcatcataag cgaatccata
    19141 tgtctcgaga aatgccttat cactttctgg catttgctca ttattgtagt gaatacggcc
    19201 ttgtatgtcg tcattttcat atctatatct tggatcgttt ctataaaatt ctaagactga
    19261 caagtcaaat gatacataag aaagttgtgg ttctcctaaa gcaaggcgta gcttatatgg
    19321 ttcagattta tagtcatcag gattaaccat ttttttaagt attaatttgc ctgggtacaa
    19381 gcacatatac ccaggttcgt ccgcattagc cttctctagt tgaacttcga gcggctcgaa
    19441 ccctattctt ataatatttg ggttgatgtc tgtctttcca tcaataatgc gaatcaagtc
    19501 actttttata agatcttgaa tcaatggttt aatctcggtc cattctttcc caactacgga
    19561 taacagattc caaatttgaa tgccattgaa atcattcgat tttagatagt attctgctac
    19621 cgtttgctct attattttct tcactttatt acctttcgat tgtttataaa acgccccaag
    19681 atgtcttcac ctcccattta gccaagacat tgtacctagt accccgaacg tatctaaagt
    19741 tctccaaaga gcaattttgg cggaatacca aatgtaagga accagatata ttcttttagc
    19801 caaaacacca ttaaatagat tcctgcctat atctgctatt tcttgaaaaa agatctttga
    19861 tttcctctaa actaaaccct aactccgcca ataaacatac ttctaaacac attttcagtt
    19921 tctgggtaag atggtaaagg tcttctccgc tgacagcacg ttcttttagc tccttatcat
    19981 gatgtgtctg ataatttctg gtattaacca ctttttcaat aaaggcatct tcatcttcta
    20041 taaatttatc taaaattttt cgatatttat cgaaaaattc tttcaacctt tttcttaaag
    20101 aaaattcatt accgtacttc aaatattcct tcagtctatc ttttaagtca cttccgacat
    20161 tctctggaat tgcagttaca agagctttat ataccgcttt ataaccttca tctgataaat
    20221 attctcctcc gcaggtgcat tgatgaaacg actctatggc ttggatcaga cttaaaaaat
    20281 gatgttcaag atacatgcga ggattatata acgttccaaa gtataaatca taaacaggct
    20341 ctaatgaatc cttttcccca aaccaagtcc tcaggaaaga ctcaaatcta ttggagatat
    20401 ctttgaaagt aaaaagcata tcaactggaa gtaaagtttt agacgctttt gagatatctg
    20461 gcagtctgta aaatatttca actggtggat aacaaatttt accctccacc atctctttgt
    20521 ttacttctgt tagccctgtg atggctaatg gataagcagg ctctgtaatt ccgagagtca
    20581 aaaaattctg aatttgacgg ataactttta tgcaatcttc aaaagattta tcttcagaag
    20641 tttcaatttc tatctctgtc tcctgtttga tggccgcttc tttctgaaca aaagcaagtg
    20701 atggccctgt aacttggata ttaatcaata ttttgagccc gtcaccaata tcagcctgaa
    20761 aggattcagg caacttatat tttattatta tttctttttc ttcccctaaa ggctttatgt
    20821 caaacccaga aatatttacc cattcgtcca aatgcaagca atgaacagag atacttttaa
    20881 atcttatgtc ctctgacttc tgaaagtgaa cacctataaa aacaaagtta gcgtagaata
    20941 aagaggtttg aaatccttcg gagctaaaac ttgacttagt ttcaaaacat ttgtatagag
    21001 taatattttt cccattagaa gagatgccca atacaatctc gggttctaac gttttgttca
    21061 tatttttgat atcttcaatg gaacctatta agtccaaaat tgctccttcg ttgggggtga
    21121 atctaagtgt acctgaaatc cgtttttcgg gattatcagg aagccaccat atacccttgt
    21181 attcaaattc tttaatcatt tttcatctcc aatagcattt cagataacat tttgcttttt
    21241 tgcaaagtgt tagatattca gctaaacgaa actaaccgca aacacgctat tagctggtat
    21301 gcaactgcta ttttttgtct aattttcttg aacatctctc tatacgcttt tcccaatcac
    21361 cagcccaccc ttgctctaaa gctgcctttg agatttgaat tgcagcttca aaatttttac
    21421 gtttttcctc aattatagcc aactgcccaa aacccttatg agctggcaaa gggctgtctt
    21481 tatattctct ttgaaaagca gctttagctt tgggggatat ttctatctgc tgtttacaag
    21541 cctcaattgc tttctccagt gcatcagggt cgttatctcg atttctgtag tatatttcta
    21601 ttttgctctg gtacaaaaag tggatgtcta aaatgttact tgattcatct atcatttctt
    21661 ctgccttttt gataattcta tatgctattg ttctgtcatc ttttttacta aaccagctcg
    21721 caagagctga taataaaccg atgggagttt cgcttgtgct agcaatattg cctttaatta
    21781 aagattctcc gcttgaacca aatggttgaa atgtttttat gatatgttct ctttcctctt
    21841 ctgaaaaatt tgataaccac caatcaccaa gcccaaaata tccgattttt ccttgaatct
    21901 gctttgactc ttttttgaaa atattaaata tgcccatatt tattatcctc taaaaaataa
    21961 ataaaaacag tctaagcata acatttccgg caactcgtac atagacaaac tatcttctct
    22021 atttcagcgg atgaattgcc agaactcccc actcttataa taaattctgt catttagtac
    22081 ccttccaggt ttaaactacc taacgaactt tttgtgattt ctatgaccca acaattcttg
    22141 aatatatctc aacttcccga caaatacgtt gtagagttaa tcgttccgac cgcaatatct
    22201 ctggttttta tcccttcaaa caaccacgtt tcaggtttgt atttggtcca catcacccca
    22261 aaacactcag agccctcttt agtagcacaa tgcgtatggc cttttcccta tagagcttct
    22321 gatgcgaatt aatgtttgtt tcacatctca tctgatgaaa gaattgttac aactccttcc
    22381 cggattgcga tgatgagcaa cttttgtctt tgcgaggtct gcgaggtctt ttagctcgta
    22441 tataattttc ctttgagcat tatatcatag ttaaatttca acggcattga tagcccgatt
    22501 caggataaac ccatcggctt gccacccggt tttagcagca atataaattg ttagatttct
    22561 cttatagcct ttggctactc ttcttcccag ataaacaaaa ttgagatggt ttcagtaact
    22621 tttggacact tcaaggagat actctccttt atggttctgg aacaattgcc tgttgtctgg
    22681 aatatttata aataggcagg gttttaaaat tatgaaagat acagcaaaag ttttttaacg
    22741 ccaacgtaga attgaatctc ccatcgttgc aagcaatatt tgtcttttga agtaaaagca
    22801 ttaaccgttt ctatcagcct tagggagagt ttatcttata agaagaagga ggaagagcaa
    22861 atgcccagag tgacttttaa acttgattat ccacaagctc aaagtgtttt tatagcagga
    22921 ggtttcaata actgggacac ccaggcatat cctatgcgca aaactcagaa aggtcactgg
    22981 agttgtaccc tcacactccc ctgcgggcga tatgaatatc gctatattgt ggatggagaa
    23041 tggtataccg acccatctac tcctcgcgtt cccaatgaat acggctcaga aaattcggtg
    23101 attgaagtca aagggaggga aactaaaaag ggagggtaat aattttttag gaggtattaa
    23161 aatgtgcaaa gtaatttttg aagtttatgc tcctcgggca aaatcagttt acctggtagg
    23221 ggattttaat gagtgggata ttagagcctg ccctatgaaa aagaaaaatg ggggctgggc
    23281 tgccagcctc gatcttcctc ctggaaaata tgaataccgg tatctaattg atggaggatg
    23341 gtataatgac cctcaaacat accgggtttt cactgactgg ggagagaatt ctatactgga
    23401 ggttgaagaa gattgttcat caatctccgt ttagctattt ttatagttaa ccgcaaaact
    23461 aatcataatt actataaagg ggtaaacccc cttataacct catcattacc cataagtttt
    23521 ttctggacac catagaaggt tttcaaaaca gataaatg
//
LOCUS       DAOSVE010000019        23311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_1431_length_23311_cov_76.613433, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000019 DAOSVE010000000
VERSION     DAOSVE010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 23311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23311
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1431_length_23311_cov_76.613433"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="PK016_02870"
     CDS             <1..518
                     /locus_tag="PK016_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02048.1"
                     /translation="KRPQRAVEVQHYLDIWLSELYQRKPHSALSGKTPEQVFKEDDRP
                     LRWATKEELDWAFTFTEQRLVDKTGCISFSGIPWEVGEDLIGMKVEVVSRCDNPEELE
                     IFHEGFEPRKIKPLKITEHTNPHRGVLPSEENPPSTSRELDAATREYEKHKDFSQTAI
                     SYRSLIEGEGQ"
     gene            515..1315
                     /locus_tag="PK016_02875"
     CDS             515..1315
                     /locus_tag="PK016_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXL02049.1"
                     /translation="MIETFYGFTKTPFTKEVTEEAFFENEQIEEILARLHYAASKQWF
                     ALLTGDCGTGKSTLVRKLSSTLSTSDFQVLYLSDSQLKPYHFYHGLLEQLGVTGRYYR
                     GDAKRLLHREVEILGGVQGLKPVVIIDEAHLLEREMLEEIRFLLNSQMDSRNPLALIL
                     VGQTEIRDKLRKPVYTAIAQRLDLHCHLAHFDEAGTRSYLLHHLHYAESPSDIFSDEA
                     IKEIFRYSAGSARLINKACTHCLMYGEQHQKKIIDDHIVRYVIEKELL"
     gene            1464..1571
                     /locus_tag="PK016_02880"
                     /pseudo
     CDS             1464..1571
                     /locus_tag="PK016_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012264517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1884..3419
                     /locus_tag="PK016_02885"
     CDS             1884..3419
                     /locus_tag="PK016_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL02050.1"
                     /translation="MPAIEKVEKTVEVAFHSVTKRFPPDILANDHVSFGVKKGTVHAL
                     LGENGAGKTTLMNILYGLLPPDEGEIIIRGKPTKISSPREAINLGIGMIHQHFKLIPA
                     HTVSENIILGLASTPFFFPAEKAESEIRSISEKYGLKIDPRARIWQLSAGEKQKVEIL
                     KALYRKAEILVLDEPTSVLTPQEVEELFATLRLMKEEGKTCIFITHKLNEVKEIADVV
                     TVMRKGKVVSTILPSQVSLEELARMTVGKEVISVVPRKTVSDKIILQLQEVSAMGDRG
                     FPALLNLSLTVKEGEIVGVAGVAGNGQKELAEVIVGLRQVTGGHIFLEDREITNLLPR
                     DIASLGVAYIPEERHLGLVGEMSVAENLLLRGYSQPPFSQGFWVDWAKAEAYSEKLIS
                     NYHINPPLADIPVRLLSGGNRQRVIIARELERKPKLVIASNPTAGLDVSGVEAIHRLL
                     VKAQEEGAGVLLISGDLDELLKLSNRIVIVFRGEIVGEKEKEHWTEEDRQEIGLRMGG
                     IRR"
     gene            3420..4526
                     /locus_tag="PK016_02890"
     CDS             3420..4526
                     /locus_tag="PK016_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL02051.1"
                     /translation="MGRRKLAQFHVELSLQPTLLSVVFLSFLSIVLAFACGSVIFLFY
                     HISPWMGYKTFFQGAFGSWYAFSETLKRTIPLLLVGEGLALAFRAQVLNIGAEGQILL
                     GAVAATWVALFSGLSSFLLLPVMFLLGFVAGAGWALIPAFFKTRFAVNEVLSTLMLNY
                     VASNLVLYLIGGPWKGKGVRGFTYTDTFPEAAWLKTMGITRVPYFTLLVGIVVAVGGY
                     FFLKKTALGFELRIVGSSPETAKLLGINEEKIILFTMLLSGGLAGFAGVGEVAGIHHL
                     LRHPDHISLGYGYTAIIVAWLAQANPLATILTSFLFGALLSGGDALRVSLGIPFQVVS
                     VFNGLILFFLISTEHLSRYRIIWGNKKVVEKSGS"
     gene            4516..5445
                     /locus_tag="PK016_02895"
     CDS             4516..5445
                     /locus_tag="PK016_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL02052.1"
                     /translation="MALELVLGTLNRALAYGTPLLLATLGEIYVERAGVLNLGIEGMM
                     MMGAFSSFVAAYTTSNPWLGVLVGAMVGGLFSLIHAFSSITLRANQTVSGLALTMLGT
                     GIAETLGRSWEGKILASPIRPFSEDFLSWFPLWMKTLFQDSNLLVVGSIILALILWFF
                     LFKTKPGLFLRSCGENPAACDALGINVWKLRYLAVFWGGIMAGLGGAYISVVYRPAWS
                     SGMTQGMGWLAVALTIFSFWNPIYAPLGAYLFGALFHLAFRLQVFVAPEILNSLPYLF
                     PVVALVFVSRLAFRKKIGVPGSLGRPYERGESE"
     gene            5479..7527
                     /gene="tkt"
                     /locus_tag="PK016_02900"
     CDS             5479..7527
                     /gene="tkt"
                     /locus_tag="PK016_02900"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015449178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HXL02053.1"
                     /translation="MEASVEQLTVKTIRMLALDMVQKAKSGHPGLPLGAAPLGYVLFR
                     RIMKHNPRNPRWFNRDRFILSAGHGSALLYALLYLSGYEKMSIEELKNFRQWGSRTPG
                     HPERCIDCGIETTTGPLGQGFATGVGVAIAEAHLAARFNRPEFPIIDHYTYALVSDGD
                     LMEGVAQEAASLAGHLKLGKLIYLFDNNRVSLSGETAICFTENIKMHFEAYGWQVLEV
                     KDGNDLEELTQAVEEAQKDKSHPSLIMVSTHIGYGSPKQDKFEVHGSPLKPEEVLETK
                     KFFGWPPDQDFHIPEEALNHMREIIKKGEEKEGEWNSLFSSYAREFPELAQELTMLMK
                     GELPPGWDKDLPVFDPASGSVATRSAGGKILNALADKISYLIGGSADLDPSTNTVLKG
                     KGSFQAPGSCPPGAQGLVEGPLGYEGRNIAFGVREHAMGAILNGIAIHGGLIPFGATF
                     FVFSDYMRPAVRVAALSRCKVIYVWTHDSVALGEDGPTHQPVEQLMSFRVMPEIVCIR
                     PADANETREAWKVALERKEGPVALILTRQNVPVIDRDKYGKAEGLKRGAYIIADCAPH
                     LPQLIIIATGSEVSLALEAYEKLTFEGVHVRVVSMPSFELFEEQSEAYRREILPPEVK
                     KRMAIEAGATLGWYKYVGEEGKVIGIDRFGASAPGKVVLENLGISLENILAQAQSLLG
                     "
     gene            8144..9310
                     /locus_tag="PK016_02905"
     CDS             8144..9310
                     /locus_tag="PK016_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HXL02054.1"
                     /translation="MSKKVILVMVLLVSFLFAGVAFSEEAKLKAGFIYVGPIGDLGWS
                     HAHEQARQVLEEVYPWLDTIYVEAVPEGEVEGIIDRLVNQEKVDVVVTTSFGFMDGTL
                     NAAKRYPDKIFFHCSGFKRASNLATYMADFHQIYYLNGIMAGALTKSGKIGYVGTFPT
                     PELKRHINAFTLGVRRVNPQAEVNVRWLQSAWYDPQGAKEAAEALIAEGIDVLAFTED
                     SATVAQVAEEKGLPSFGHYSPMLSYAPNHLVSGQLVHWETIYLDFFAKVYAGLYNNEN
                     LENVDYWWLLNDGAVELGADFGVPINPAFEETLKSYMVSDPIYGDVSAYDLVFKLEKA
                     MADPEYTFSPFTGPIKDRKGNLRVPSSVRLSVEDLITMEWAVEGVVGPWPGEPE"
     gene            9422..9496
                     /locus_tag="PK016_02910"
     tRNA            9422..9496
                     /locus_tag="PK016_02910"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9454..9456,aa:Gln,seq:ttg)
     gene            9656..10849
                     /locus_tag="PK016_02915"
     CDS             9656..10849
                     /locus_tag="PK016_02915"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HXL02055.1"
                     /translation="MDKMSWWREAKFGMFIHFGLYSILGRGEWVMYQERIPKEEYRKL
                     ADIFNPGNYNPEEWVKLAKKTGMKYMVLTTRHHDGFSLFDSQVSDFTAPRSPAGRDLV
                     KEYVDACRKYDMKIGFYYSLLDWRYDTYFKGPEKDPQGWKELVGYVHAQVEELMTNYG
                     KIDILWYDGAWPYSAEDWQSQKLNEMVRRLQPGIIINNRSQLPEDFDTPEQYVPGHFP
                     NPDAPERDWESCMTLNDHWGYCKGDNNWKHPRIVIWNLARCASGGGNYLLNVGPEPDG
                     TIPQESLDILEQVSKWVDKNRESIYGTVRTKVGFPHGVTTLKGNKLYIHIFYWNSEFA
                     LAPCKIDVKRAYFLETGEEVKYTVKGERIIFSGLPDKAPEALDTIIVLEFEGGVTALE
                     PFRAL"
     gene            11074..12624
                     /gene="purH"
                     /locus_tag="PK016_02920"
     CDS             11074..12624
                     /gene="purH"
                     /locus_tag="PK016_02920"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HXL02056.1"
                     /translation="MIQIKTALISVSDKTGLEELATFLQKQGVEIIATGGTARFIRNL
                     GIEVKEVSEVTGFPEILGGRVKTLHPVIEGGILARRNNPEDQEDLGKHQIKPLDLVVC
                     NLYPFLKMIEAGETSLEKVVEEIDIGGITLLRASAKNFRYVVVVSSPCQYQELMEEMV
                     KNGGGFPEDKSQKWAIEAFLQTSAYDSYIANYLTKLTYPEEELFYPQFSLLLRKKSPL
                     RYGENPHQRGAFYEDLSLDPQGFMRYMEHWGGKELSFNNLYDVQAAFGLVREYEEPAV
                     VIVKHNNPCGVATDSNLSVAAQKALSGDPVSAYGGIVAFNRPVDKESAEVFRKLFIEV
                     VIAPSYEEDAREIFSSKKNLRVIQAPLSSPSYFDLKKVDGGFLLQDKDIIDLDKDKLQ
                     VVTEKKPTPQEEEDLLFAWKVAKHVKSNAIVLAKDHQTVGIGAGQMSRIDALYIALLK
                     AQGREKGAVLASDAFFPFSDVVEEAGKNGITAIIQPGGSIRDEDSIQACNKLGISMVF
                     TGIRHFRH"
     gene            12658..14514
                     /gene="mnmG"
                     /locus_tag="PK016_02925"
     CDS             12658..14514
                     /gene="mnmG"
                     /locus_tag="PK016_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HXL02057.1"
                     /translation="MEKYYDVIVIGGGHAGCEAAHSSARMGAKTLLLTTTIHHLALMP
                     CNPAIGGPGKGQVVREVDALGGLMAKATDLSTIHIRRVNTGKGPAVQTLRAQTRRDLY
                     SLIMRKFLESVPNLHLFQGEATEILQDEEGKIQGVRVKNQTTFFAPAVVLCTGTFLNG
                     MIKIGELSYPAGRQGEFPSVDLAYNLRNMGLQLGRLNTCSPPRLDRRTIDFSSLEEQK
                     SDEEPLCFSFTGVPQVYKGASVFIAHTSKETRDIIKANFHRSPLLYSASLTSSVRECP
                     SLEDKVYRFPERENHLLFLEPEGEESSEIYVQGLFTSLPEEVQWEIVHSIRGLERAHI
                     IRPGYGIEYDYVLPTQLRASLECREIPGLFLAGQINGTSGYEEAAGQGIVAGINAASY
                     VKNQPPLILRRDESYIGVMVDDLVTKGVDEPYRLRTGKVEYRLVVRHSNADLRLSFYA
                     YERGIISKKEYERVEEKRDKIQKEIERLSHLTIFPTAQLQTLLAQKGTNPVKEATKAS
                     FLLSRPEISYRDLAPFDWGRPFLEPQVIEEVEIEIKYRGYIERQKREIEEFKKIENKI
                     IPPDFDFSSVQVISQEAREKLQKIKPATLGQAARVAGVTPSDIVALYILLEK"
     gene            complement(14515..15831)
                     /gene="glnA"
                     /locus_tag="PK016_02930"
     CDS             complement(14515..15831)
                     /gene="glnA"
                     /locus_tag="PK016_02930"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HXL02058.1"
                     /translation="MSSNFQDVLKKAQEKNINFVRLQFVDVLGRAKNVEIPIKKLPAA
                     LEEGVNFDGSSIQGFVRIEESDMKLIPDPDTFIICPWEEDSVARIICDVRRADGSVFE
                     GCSRTNLKRVLKEVEKRGWDFEVGTEAEFFIFKYENQKILVSDRGSYFDLLPLDLEES
                     LRRDMVIALEDLGFNIEASHHEVAPSQHEIDFEYNKALKIADSLITLKLAVKTLALKE
                     GLCATFMPKPLYGVPGSGMHTHLSLFRGEENLFYDPEGPDGLSKIARGFIAGLLQHAP
                     ALTAITNPLVNSYKRLVPGYEAPVYIAWAEQNRSPLVRVPPQRGKKTRAELRNPDPSC
                     NPYLAFAAILRAGIDGVDKELDPGKPCNSVNLYRMSREEREEWNIRSLPRNLREALEE
                     LEKDSIVREALTPHILDFFLEAKYQEWEEFESAVHPWELERYLELY"
     gene            complement(15821..17275)
                     /gene="argH"
                     /locus_tag="PK016_02935"
     CDS             complement(15821..17275)
                     /gene="argH"
                     /locus_tag="PK016_02935"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HXL02059.1"
                     /translation="MTNLWGSRMEKDLDREVMEFSTSIPEDFLLYKYDLWGSAAHCKM
                     LEKVGIISEKESKEILRGLRKIFEEIEEGKIDPSLFEDIHSLVEIRLREITGEEIGGK
                     LHTARSRNDQIVLDERLFLREKIEEVIEGINSLQEALLQKARENKDVVMPGYTHLQPA
                     QPVLFSHHLLAYFFMLERDVERLEDTRKRVNVLSLGAGALSGTSLPIDRQYVARLLAF
                     PRVQENSMDAVSDRDFILEFLGDLSILFLHLSRWGEEIVLWSSPAFHFLKIDDAFTTG
                     SSIMPQKKNPDVAELLRGKTGEVVSSWVSLAITLKGLPLTYNRDLQQDKPPLFRAVKE
                     ALSSLRIAARLAQNIFPDRESMKKALSTGFLTATDLCEYLVERGVPFRQAHTWVGKIV
                     KKLSSSGKHLRDLKKEDWGEYQDLFPQNWKDIIDEEKSVRRKISEGSTSPQEVEREIK
                     MGEEFLAQTRERVSSWRKIEKESWENLVGENCVL"
     gene            complement(17272..18477)
                     /locus_tag="PK016_02940"
     CDS             complement(17272..18477)
                     /locus_tag="PK016_02940"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009333268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HXL02060.1"
                     /translation="MSQQKVVLAYSGGLDTSVAIRWLQEQFNAEVYALTLDLGQGKDL
                     EEVRKKALSIGAKEALVFDTREEFLNEYVLPALGAQAIYEGRYPLATALSRPLIAKYL
                     VKVAEEKGATIIAHGCTGKGNDQVRIEVSVMALNPDLQVVAPARVWKWTREEEIEYAH
                     ANGIPVPVTLEQPYSIDQNIWGRSIECGVLEDPLVEPPEEVFEWTKNPQDAPLEPTYL
                     EIGFEEGVPRLLGGKYYSLLSLVEELNRVGGENGFGRVDHVENRLVGIKSREIYECPA
                     ALILLEAYRDLQNLVLPREVIHFKPHLEEEYAQLVYEGLWYSPLREALDAFMKSFLPR
                     MTGVVRIKLYKGSMQVVGRKSGYSLYDWGLATYDRGDLFDHRASEGFIKIWGLPTRVQ
                     ATLEKRKKL"
     gene            complement(18500..19687)
                     /locus_tag="PK016_02945"
     CDS             complement(18500..19687)
                     /locus_tag="PK016_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HXL02061.1"
                     /translation="MTSSEIIEKEARYFLGTYSRLPVVLERGEGCYVFDLEGKRYLDL
                     MAGIAVNILGYSFPPLKSAVKEEVDKLLHASNLYYSVPQVELAERLVKKSGFSQVFLV
                     NSGAEANEVALKMARFYRKRKYGKGYKFISFYRSFHGRTLLTLGLTAQEKFHKDLDPF
                     PPGIEYATLNNLESVEKVLDDEVCAIIVEPVQGEGGIYPCNKEFLLGLRKLCDEKDLV
                     LIFDEVQCGMGRVGKFFAFESFEVRPDIVTLAKGLGGGLPLGAVLVDEETSSSVHRGD
                     QGSTFGGNPVCSRAACVVVDEVSKESFLSTVREKGKYFEEQLKKLASIFPMVKEVRGR
                     GLMLGFEVPGKAKKITEEMLKEGVLVNACNDDTVRFLPPLIITQEEIDEGVEALKKVL
                     ERI"
     gene            complement(19719..20516)
                     /gene="argB"
                     /locus_tag="PK016_02950"
     CDS             complement(19719..20516)
                     /gene="argB"
                     /locus_tag="PK016_02950"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HXL02062.1"
                     /translation="MEIVVKIGGKNIDFKNEDCFQVLTQLIREGIKLALVHGGGAQIT
                     KFLEKEGRESQFVEGLRVTPPEDMDITEMVLSGLLNKMLVGIFHRQGVPACGISGRDG
                     FLFEGEKYYLTSGGKKVDLGRVGEIKKVNPRLVRVLWEGGFLPIISPVAADKEGESLN
                     VNADWAAARLAVALQAEKLFLFSDVPGVLQDMEDKNSLIEVLPRAGIETLKKEGKIRG
                     GMIPKLEMAEEALQGGVKEVFIGEGKEMQNLSFFLAGGKMKGTKVVL"
     gene            complement(20676..21887)
                     /gene="argJ"
                     /locus_tag="PK016_02955"
     CDS             complement(20676..21887)
                     /gene="argJ"
                     /locus_tag="PK016_02955"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HXL02063.1"
                     /translation="MESWKILEGGLDNVSGFSSYGINCGIKKEKNDLAAILSDTPATA
                     AGVFTSNRFSAAPVLVTREHLLKERKLQLVVANSGVANACTGERGIEDARKMADFAAS
                     CFKVDSHLVAVASTGVIGEFLPLPKIEKGIEELSRILPVKKSSREAVKAIMTTDTFPK
                     ERALSFSWKGKTVHLGGIAKGSGMIKPNLATMLSFIVTDLGIDAGTLNQVLKEVVDES
                     FNRVTVDGDTSTNDMVLILANGKSGIEAQEKDEVFPLFREALLWLAQELSRDLARDGE
                     GATKFVEIIVQGAKNKEEARRGAFTVAESPLVKTALFGEDPNWGRIMAALGRSGIEID
                     PHKVNLEINGVTLVEGGMKAKGVGREQGQEAMKKKELRIIVDLGIGEGEFGVWTCDLS
                     LDYVRINSHYS"
     gene            complement(21898..22929)
                     /gene="argC"
                     /locus_tag="PK016_02960"
     CDS             complement(21898..22929)
                     /gene="argC"
                     /locus_tag="PK016_02960"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HXL02064.1"
                     /translation="MIHVGVMGATGYTGLELLRILSHHPGVEVCWVGSESFPGMTLED
                     YCPAWRGRSSLTLQKQEVKKLEGIEVIFLGLPSGVAMDYVPSLLQKGIRVIDLGADFR
                     LKNLEAFQKYYQLEHRVPHLLREAIYGIPEIYEESLKEARLVANPGCYPTSVIIPLYP
                     FLKEGVVSPSIIVDSKSGVTGAGKKATEDNHFCEVDENFKAYKVGEHRHQPEIKEQLE
                     LAKGGEVNLVFTPHLLPLKRGILTTIYLTLEKDMKEEQLLSIWKDYWGRKPWVRIFPL
                     HRFPELRWVVGSNFIDLGLKRVGENQLIVISALDNLVKGAAGQAVQNLNLMFGRGEKE
                     GLPEDVLYP"
BASE COUNT         6503 a         5012 c         4947 g         6849 t
ORIGIN      
        1 taaaaagacc ccagagagct gtagaggtac aacactatct ggatatctgg ctatcggaac
       61 tctaccaacg taaaccccat tctgctctat ccggtaaaac ccccgagcag gtgttcaaag
      121 aagatgaccg tcccctaagg tgggcaacta aagaagagct ggattgggct tttactttca
      181 ccgagcaaag actggtagat aaaactggct gtatctcttt ttccggtatc ccctgggaag
      241 taggagaaga cctaataggt atgaaagtag aggtagtctc ccgatgtgat aaccccgagg
      301 agttagagat tttccatgaa ggatttgaac ctcggaaaat taaacctcta aagattaccg
      361 aacacactaa tccccacaga ggtgttcttc catctgaaga gaatcctcct tccacttctc
      421 gggagttaga tgctgctacc cgggagtatg agaaacataa agacttctcg caaacggcca
      481 tctcttatcg ctctcttata gaaggagaag gacaatgata gagactttct atggctttac
      541 taagactcct tttaccaaag aggtaactga agaggctttc tttgagaatg agcaaataga
      601 agagatcctg gctcgtctcc actatgccgc ttctaaacaa tggtttgccc tgcttaccgg
      661 tgactgtgga actggtaaat ccactctggt tcggaaatta tcctctactc tttctacctc
      721 tgatttccag gtcttgtatc tctctgactc tcagcttaaa ccttatcatt tctaccatgg
      781 actattagaa caattaggag taactggccg ttactaccgg ggggatgcta aaagactcct
      841 ccatcgggaa gtagaaatcc taggaggagt ccaaggttta aaaccagtgg taatcataga
      901 tgaagctcat cttttagaac gggaaatgtt agaagagatt cggtttcttc tcaactccca
      961 aatggattcc cgaaatccct tagctcttat cctggtggga caaactgaga ttagagataa
     1021 actcagaaag ccagtctaca ctgctatagc tcagcgcctt gacttgcact gtcatctagc
     1081 tcactttgat gaggctggta ccaggtctta cctcttacat catctccact atgctgagtc
     1141 tccctctgat atcttctcag atgaagccat aaaggagatt tttcgttatt cagctggtag
     1201 tgcccgattg attaacaaag cctgcaccca ttgtttgatg tatggggaac aacatcagaa
     1261 aaagattatt gatgaccata tagttaggta tgtgatagag aaagaactcc tgtaagctga
     1321 ctgtgaggac tgaggtattc tcaaggaaga gtatctcagt cctttcttat tcttggatac
     1381 aatatctggc agttagagga cattttatcc tggcaactac cagacaaaat agcctggtag
     1441 aagcaaagtg gagaaaagaa cagggaatag agatagaggt cctatatcta cctccctact
     1501 cccccaactt gaatctggta gaaaggctat ggaagtatgc aaagaagaaa atacttggag
     1561 tttattatga tgaactatgg aaattcaaat cagcagtgaa gaacttcttt gaaaacacag
     1621 taaaagacaa agcttataac atggaattga agcaattcat aggaactgct tttcaggtcc
     1681 taagtagtta aattgggatt tcagatttaa agttgattta gtatagttct gtttcctctc
     1741 ccagtggttt ttatcttttt acgtttttct gtttttttct tttaatccct taaggggggt
     1801 gataaggggt cttcccctat agtataatac tctggtgagt ctattgagaa ataaggtata
     1861 atcctatatg gagatgaatt aatatgccag caatagaaaa agtagaaaaa acagttgaag
     1921 ttgctttcca ttctgttacc aagcgttttc ctcctgacat tttagccaat gaccatgtgt
     1981 cttttggagt gaaaaaaggt actgttcacg ctttgctggg agaaaatgga gcgggtaaga
     2041 cgacgctcat gaatattctt tatgggcttc ttcctcctga tgaaggagaa ataattatca
     2101 gaggaaaacc gacgaagatt tcttctccca gagaggctat taacttagga attggcatga
     2161 ttcatcagca ttttaagctc attcctgctc atacggttag tgagaatatc attttgggat
     2221 tagcatctac tccttttttc tttcctgcag aaaaagcgga gtccgagatc aggtctatct
     2281 cagaaaaata tggtttgaaa atagatcctc gggctcgaat ctggcagctt tcagcaggtg
     2341 aaaaacaaaa ggtggagatt cttaaagctc tctatcgtaa agctgaaatt cttgttctgg
     2401 atgaacctac ttcagttttg actccccagg aagtggaaga gctatttgct actctccggt
     2461 tgatgaaaga agaaggtaaa acctgtattt ttatcactca taagcttaat gaggttaaag
     2521 agattgccga tgttgtgacg gtgatgcgca aagggaaagt agtttctact attcttccct
     2581 cccaagtttc tctggaagaa ctagcccgaa tgacagtggg gaaagaagtt atttctgttg
     2641 ttccccgcaa aaccgtatca gataaaatta ttcttcagct tcaagaagtt tctgctatgg
     2701 gagacagagg atttcccgct cttttaaatc tttctcttac agtaaaagaa ggagaaattg
     2761 taggagtggc aggtgtagcg ggaaatggac aaaaagaatt ggcagaagta atagtaggtt
     2821 tacgccaggt tacaggaggg catatttttt tagaggatag ggaaatcact aatcttttgc
     2881 ctcgagatat tgcctcttta ggggtggctt atattcctga agagcgacat ctggggctcg
     2941 tgggagagat gagtgtggcc gaaaatcttt tattgcgagg ttattctcag cctccttttt
     3001 ctcagggttt ctgggtggac tgggcaaagg cggaagctta cagcgaaaaa cttatttcta
     3061 attatcacat caatccgcct cttgccgata ttccagtgcg gcttctttcg ggggggaatc
     3121 gccagagggt aatcattgcc cgagagttgg agcggaaacc gaagctggtg atagcttcca
     3181 accccactgc tgggttagat gtttctggtg tagaagctat ccatagattg ttggtaaaag
     3241 ctcaagaaga aggggcgggg gttttgctta tttctggaga cctggatgag ctcttaaaac
     3301 tttctaatag aatagtgatt gtgttcagag gagaaattgt gggagagaaa gaaaaggagc
     3361 actggacaga agaagaccgg caagaaattg gcctgaggat gggaggaata agaaggtaag
     3421 tgggcaggag aaaacttgct cagttccacg tagagctatc actacaacca actcttctct
     3481 cagttgtatt tctctctttt ttgtctattg ttcttgcttt tgcttgtggt agcgttattt
     3541 ttttgtttta ccatatttct ccctggatgg gttataaaac cttttttcaa ggtgcttttg
     3601 ggagctggta tgctttctcc gaaactctga aacgaactat tcctctcctt ttagtaggcg
     3661 aaggattagc tttagctttc cgggctcaag tgctcaatat tggagcagaa gggcaaattc
     3721 ttctaggagc tgtggctgct acctgggtgg ctcttttttc tggactttcc tcttttcttt
     3781 tgcttccggt gatgtttctc ttgggctttg tagctggagc aggatgggcg ctaattcctg
     3841 ctttctttaa aactcgtttt gccgtgaatg aagttctttc cactcttatg cttaattatg
     3901 tagcttctaa tttggttctt tacttgattg gaggtccctg gaaaggtaaa ggggtgcggg
     3961 gttttaccta tactgatact tttcctgaag cagcctggct taaaactatg ggaataacca
     4021 gagttcctta ttttactctt ttagtaggta ttgttgtggc tgtgggaggc tatttttttc
     4081 ttaaaaaaac cgctttagga tttgagctcc gcattgtagg tagtagtcct gaaacggcga
     4141 agctcctggg aataaatgaa gaaaaaatta ttctttttac catgctcctc tccggagggc
     4201 tagctgggtt tgcgggagta ggagaggtcg ctggtattca tcatcttttg aggcatcctg
     4261 accatatttc tttaggctat ggatatacgg cgataattgt agcctggtta gctcaagcca
     4321 atcctctggc tacaatttta acctccttct tatttggtgc tcttctctcc ggtggtgatg
     4381 ctctccgggt atcgctgggt attccttttc aggtggtgtc ggtgttcaat gggcttattc
     4441 tcttttttct tatttctacc gaacacttga gccgatatcg tatcatatgg gggaataaga
     4501 aagtggtgga gaaaagtggc tcttgaactt gttttaggaa ccttaaaccg ggctctggct
     4561 tatggtactc cccttctttt agctactctg ggagagattt atgttgagcg agcgggagtt
     4621 ttaaatctgg gaatagaggg gatgatgatg atgggagcat tttcctcttt tgttgcggcc
     4681 tatacgacct ctaatccctg gctgggggtg ttggtaggag ccatggtggg gggattgttt
     4741 tctctaattc atgccttttc tagcattact ctacgtgcta accaaactgt gtcagggctg
     4801 gctctcacca tgttaggaac ggggattgct gaaactttag gaagaagctg ggaggggaaa
     4861 atacttgcct cacctatcag acccttttcg gaggattttt tgtcctggtt tcctttgtgg
     4921 atgaagaccc tttttcagga tagtaatctc cttgtagtag gaagtattat tctggctctg
     4981 atactctggt tttttctttt taaaactaag ccggggcttt ttttacgttc ttgtggagaa
     5041 aaccctgcag cttgtgatgc tctggggata aatgtgtgga aattgcgcta tctagcagtt
     5101 ttttggggag gaattatggc tgggctagga ggagcatata tttctgttgt ctatcgtcca
     5161 gcatggtctt cgggtatgac tcagggaatg ggttggttag cagtagcatt aactattttc
     5221 tctttctgga atcctattta tgctcctctt ggcgcttacc tttttggagc attgtttcac
     5281 ttagcttttc gcttgcaagt gtttgtagct ccagaaattt tgaattctct gccctacctt
     5341 ttcccggtag tagctctggt atttgtttct cgccttgctt ttcgtaaaaa gattggcgtt
     5401 ccgggaagtt taggaagacc ttacgagcgg ggagaatcag agtaaataaa tataggaatt
     5461 aagaaaggag tgaaaaaatt ggaagcctct gtggagcagt taacagtcaa aaccattcgc
     5521 atgctggctc tggatatggt gcaaaaagca aagtctggac atccgggatt acctctgggt
     5581 gctgcacctc ttggctatgt tctctttcga cgaattatga aacataatcc tcgaaatccc
     5641 cggtggttta atcgagaccg ttttatcctc tcagcagggc atggttcagc attgctttat
     5701 gcccttcttt atctgagcgg atatgagaaa atgagtattg aagaattaaa aaatttccgc
     5761 cagtggggga gccgtactcc tggccatccc gagcgctgta tagattgtgg tatagaaact
     5821 accactggac ccctaggaca gggttttgca accggggtag gagtagcaat tgccgaagct
     5881 caccttgctg cccggtttaa tcgtcccgag tttcccatta ttgaccacta tacttatgct
     5941 ctggtaagtg atggggattt aatggaagga gtggcacaag aagcggcctc tttagccggg
     6001 catctaaagc tgggtaagct gatttacctc tttgacaata accgggtgag tctttctgga
     6061 gagacggcaa tttgttttac cgaaaatata aaaatgcact ttgaagccta tggatggcag
     6121 gtgctggaag taaaagacgg aaatgacctg gaagaactca cccaggcagt tgaggaagca
     6181 caaaaagata aaagccatcc ttctcttata atggtttcta ctcatattgg atatggaagt
     6241 cccaaacagg acaagtttga agtccatggt tcgcctctga aaccagaaga ggtactcgaa
     6301 acgaagaaat tttttggttg gccaccagac caggatttcc acattcccga ggaagcttta
     6361 aatcacatgc gagaaattat aaaaaaaggt gaggaaaaag aaggagagtg gaatagcctc
     6421 ttctcttcct acgcccggga gtttcctgaa ttagcacagg agttgaccat gctaatgaag
     6481 ggagagcttc ctcctggttg ggataaagac ctccctgttt ttgaccctgc ctcaggaagc
     6541 gttgccactc gctcagccgg aggaaaaatc ctcaatgccc tggcagataa aatttcttat
     6601 ctcatcggtg gctcggcaga tttagatcct tctactaaca cggttttaaa aggtaaagga
     6661 agttttcaag cacctggttc gtgtcctccc ggagctcaag gtcttgtgga aggacctctc
     6721 ggatacgagg gaagaaatat cgcttttggt gtgcgtgaac atgctatggg agctatttta
     6781 aatggtatag ccattcatgg ggggcttatt ccttttgggg caacattttt tgtattttct
     6841 gattacatga gaccagccgt tcgagtggca gctctctccc gttgtaaagt tatttatgtg
     6901 tggactcacg attcggtagc tttaggagaa gatgggccaa ctcatcaacc agtagagcag
     6961 cttatgagtt tccgggtaat gcctgagata gtttgtatta gaccggctga tgctaatgaa
     7021 acccgagaag catggaaggt agccctagaa aggaaagaag gaccggtagc tctcattttg
     7081 acccggcaga atgtgcctgt gattgataga gataaatatg gaaaagcgga aggactgaag
     7141 agaggagctt atatcattgc cgattgtgca ccacatttgc ctcaacttat cattattgct
     7201 acaggttcag aagtaagcct cgctctggag gcttatgaaa aacttacttt cgagggggtt
     7261 catgtgcggg tggtaagtat gccttccttt gaattatttg aagaacaatc tgaagcatat
     7321 cgccgagaaa ttctcccccc ggaagtgaaa aagcgaatgg ccattgaagc aggagcaacg
     7381 ctggggtggt ataaatatgt aggagaagag ggtaaagtta tcggcattga ccgttttggt
     7441 gcttctgctc caggtaaagt ggtgttagaa aacttaggta tcagtctgga gaatatttta
     7501 gctcaagctc aatcccttct gggataaaga gaagtaaagc gcaccttgaa aattaaatag
     7561 agtatataaa atggggtcag tgctccttta tcggctctct ctatccgttg cccctgaatg
     7621 tttctatcag gaatccaaat tataagggaa aaagactatt tagattcccg actactgatt
     7681 tcgggaatga cggggtaaga tgtcattcct gaatggttct atcaggaatc taaacttaca
     7741 agggatattt tcccccttat aacctctggg aagaaaaagg cggaggacta agaactaaaa
     7801 actaaaaact gagagagaaa actccctcgg caaaaacctt tggatgtctg atgaaagatt
     7861 tcagacataa aagtaggagg agaaaagggg gcagtctacc tttttcctct atagaataga
     7921 aaaaagtagg attgctacgg gaagaaagct acaattctta caatggcggc acgaggtgtg
     7981 atgcagaacg taaagagtag aagttactgt gttttttgta actgtacata agtaaggaag
     8041 ttgaactgga gaaataaatg agagagtcat ggttctttag ttcaaagcta catatttttg
     8101 gaatttctgg aaattttatt atcatactta gggaggtgtt tcaatgagta aaaaggtaat
     8161 tttagtaatg gttcttcttg tatcttttct ttttgcagga gtggcttttt ctgaagaggc
     8221 taaactgaag gcagggttta tctatgtggg gccgattgga gatttaggtt ggagccatgc
     8281 ccatgaacaa gctcgtcaag ttttagaaga agtatatccc tggttagata ccatatatgt
     8341 agaagcagtt ccggaaggag aagtagaagg aatcattgat cgcctggtga atcaggaaaa
     8401 agtagatgta gtagtgacca cgagctttgg ctttatggat ggaactttaa atgcagccaa
     8461 aagatacccg gataaaattt tctttcattg ttcggggttt aaacgggctt ctaatttagc
     8521 cacttacatg gctgattttc atcaaattta ttatttgaac ggaattatgg ctggagcact
     8581 aactaagtca ggaaagattg gttatgtagg aacttttccc actccagaat tgaaaaggca
     8641 tattaacgct tttactctgg gagtacgccg ggtaaatcct caagctgaag ttaatgtacg
     8701 gtggttacag agtgcctggt atgatcctca aggagcaaaa gaagcagccg aagctcttat
     8761 tgctgaagga atagatgtcc tggcttttac tgaggattca gcaacagtgg ctcaagttgc
     8821 agaagagaaa gggcttccca gttttggcca ttattctccc atgctatcct atgcacccaa
     8881 tcatctcgtt tctgggcaac tggtacattg ggaaacaatt tatctcgact tttttgccaa
     8941 ggtgtatgct ggactctata ataacgaaaa cttagagaat gttgactact ggtggctttt
     9001 aaatgacgga gcagtagagc tgggagctga ctttggtgtg cctattaacc ctgcgtttga
     9061 agaaacttta aagagttata tggtgagcga ccctatatat ggagacgtga gcgcctatga
     9121 tttagttttt aagttggaaa aggctatggc tgaccctgaa tatactttca gtccttttac
     9181 tggacctata aaagaccgta agggaaattt acgcgttcct tcttcagttc gcctttctgt
     9241 tgaagattta atcaccatgg agtgggcagt agagggggta gtcggtccct ggccaggaga
     9301 accagagtaa gctaaaagga gggaaggata ccttccctcc ttttaattat gtagaatcta
     9361 tatttagatg gtaaaaattt gattgacagc ttttttccgc tcttatataa taaaaaggtc
     9421 ttggggcgtg gccaagcggt aaggcaaggg actttggatc ccttactcgg aggttcgaat
     9481 cctcccgccc cagccatttc aggagagact cttgaaaata aagctgttct ttccggcgac
     9541 tcttttgata aattatgttt cagaatttaa cccctcatct cgcttcttta ggaagatacc
     9601 ctataaggta gctatgaatg tatttattat aacaaacaaa gaggaggtaa cctagatgga
     9661 taaaatgtcc tggtggagag aggcaaagtt cgggatgttc atacatttcg ggttgtacag
     9721 tattctcggt agaggagaat gggtgatgta tcaggagaga attcccaaag aagagtaccg
     9781 aaaactggct gatatattta atccaggtaa ctacaaccct gaggaatggg tgaagctagc
     9841 taaaaagaca ggcatgaaat atatggtgct cactaccaga catcacgacg gattttcttt
     9901 atttgatagc caggtgagcg acttcactgc tcccagatcc cccgctggac gtgacctcgt
     9961 aaaggaatat gtagatgctt gcagaaagta tgatatgaaa attggttttt attattctct
    10021 actggattgg agatatgata cttattttaa aggaccggaa aaagatcccc agggctggaa
    10081 agaactggtc ggttatgtac acgctcaagt agaggagcta atgacaaatt atggaaagat
    10141 cgatatcctc tggtatgatg gagcatggcc ctatagtgca gaagattggc aatcacagaa
    10201 gttaaatgaa atggtaagga gactccagcc aggtattatt ataaataata gaagtcagct
    10261 tcccgaagat tttgatacac ctgaacagta tgtacccgga cattttccaa accccgatgc
    10321 tccagagaga gattgggaaa gctgcatgac tcttaatgac cactggggtt actgtaaagg
    10381 agacaataac tggaaacatc cccggatagt tatctggaac cttgccaggt gtgcttcagg
    10441 gggaggaaat tacctcttaa atgtgggtcc tgaaccggat ggaacaatcc cccaggagtc
    10501 actggatata ctggagcagg tcagcaaatg ggtagataaa aacagagaaa gtatctatgg
    10561 gacagtcaga acgaaagtgg gatttcccca tggagtaaca accctcaagg gtaataaact
    10621 ttatattcat attttctact ggaacagtga atttgcctta gccccttgca aaattgatgt
    10681 taaaagagcg tatttcttag aaactggaga agaagttaaa tatacagtaa aaggtgagcg
    10741 cattatattc tcggggttac ctgataaagc tcctgaagct ctggatacta ttatcgttct
    10801 agagtttgaa ggaggtgtaa ctgctctgga gccattcaga gcactttagt aagttaaaac
    10861 ggcccggtgg atagagagta gctcttttaa aacttttttt ctcagcagtc tttctttttc
    10921 ctctaattac gggggttagc caggggtgtt ctttgtttcc ggggtcgtag aaacgaaact
    10981 ccttatagtt cttgctacaa caaatcttaa ttcggtataa tatttgattg tagttaacca
    11041 aagaaactaa agtaaatcca ggaggtgaga agattgattc aaataaagac cgctctcatt
    11101 agtgtctccg ataaaaccgg tttagaagaa ttagccactt ttctacaaaa acaaggcgtg
    11161 gaaataatag ctaccggtgg aacggccaga tttatccgta atttaggaat agaggtaaaa
    11221 gaagtttctg aggttaccgg gtttccagaa atactgggag gccgggtaaa aactttacac
    11281 ccggtaatcg aaggaggaat attagcccgg agaaacaacc ccgaagacca ggaagacctg
    11341 ggaaagcacc agataaaacc cttagattta gtggtgtgca atctctatcc ttttctaaaa
    11401 atgatagaag caggggaaac ttccttagaa aaagtggtgg aggaaattga tatcgggggt
    11461 atcactctcc ttcgtgcctc agctaaaaat ttccgatatg tggtagtagt ctcttcccct
    11521 tgccagtatc aagaattaat ggaagaaatg gttaaaaatg gaggaggttt ccctgaagat
    11581 aaatctcaga agtgggctat agaggctttc ttgcagactt cggcttatga tagttatatc
    11641 gccaactatc tcactaagct cacttatccg gaagaagaac tcttctatcc tcaattttct
    11701 cttttactcc ggaaaaaatc cccgcttcgc tacggagaaa atccccacca gcgaggagct
    11761 ttttacgaag acctgtcttt agaccctcaa ggttttatga gatatatgga acactgggga
    11821 ggaaaagaac tttccttcaa taacctctac gatgttcaag cagccttcgg tttggttaga
    11881 gaatacgaag agccagcagt ggtaatagtg aagcataata atccctgtgg agtagcaact
    11941 gattccaatc tctcggtagc ggctcaaaaa gctctctctg gagacccggt atcagcgtat
    12001 ggagggattg tggcttttaa ccgtccggta gataaagaaa gtgctgaggt attcagaaag
    12061 ttgtttatag aggtggttat tgctccttct tatgaagaag atgcccggga aattttttct
    12121 tctaaaaaga atttacgggt catccaggct cccctatcct ctccttccta tttcgactta
    12181 aaaaaagtgg atggaggttt tctattgcag gataaagata taatcgacct ggataaagat
    12241 aaactgcagg tggtgacaga gaaaaaacct actccccagg aagaagaaga tttattattt
    12301 gcctggaaag tagctaaaca tgttaaatct aatgccatcg tgttagctaa agaccaccag
    12361 acggtgggaa ttggagcggg acagatgagt agaattgatg ctttatatat agctcttctt
    12421 aaagcccagg gacgagaaaa gggagcagtt ctggcttccg atgctttctt tcctttctcc
    12481 gatgtagtag aagaagcagg gaaaaatgga attaccgcca tcattcagcc tggtggttcc
    12541 attagggatg aagattctat ccaggcttgt aataagttag gaatcagcat ggtgttcact
    12601 ggaatccggc atttccggca ttgatatcgc aacctattag ctttacagga aaaatttatg
    12661 gaaaaatact atgatgtaat tgtgattggg ggaggccatg ctggttgtga agctgctcat
    12721 tcttcagccc ggatgggagc aaaaactctt cttttgacta ccaccattca ccatttagct
    12781 ctcatgccct gtaacccagc tattggcgga ccaggaaaag gacaggtagt aagagaagta
    12841 gatgctttag gaggtctgat ggctaaagcc accgaccttt ctaccattca cataagaagg
    12901 gttaacactg gaaagggacc agcagtgcaa accctgcgag ctcaaacccg gagagattta
    12961 tacagtttga ttatgagaaa attcttagaa agtgtgccca atctccacct ttttcaagga
    13021 gaagcaactg aaatattgca agatgaagaa gggaaaatac aaggggtaag agtgaaaaat
    13081 caaaccactt tttttgctcc tgctgtagtt ttgtgcaccg gtacattttt gaatggaatg
    13141 ataaaaatcg gggagttgag ttatcctgca ggaagacagg gggaatttcc ttcagtggat
    13201 ttagcttata acttacgcaa tatgggatta cagctgggcc gactcaatac ttgcagccct
    13261 cctcgcctgg accgaagaac tatagatttt tcatctctag aggagcagaa aagtgatgag
    13321 gaacctctat gcttttcctt caccggggtt cctcaagttt ataaaggagc atcagttttt
    13381 atcgctcata cctccaaaga aacccgtgat atcatcaagg ctaactttca tcgttctcct
    13441 ctcctttatt cagcttctct tacctcctct gtccgcgaat gtccttccct ggaagataaa
    13501 gtttatcgct ttcccgaaag ggaaaatcat cttcttttct tagaaccaga gggagaagaa
    13561 agtagcgaga tttatgttca aggtcttttt acctccctac cagaagaggt gcagtgggag
    13621 atagtccact ctataagggg gctggagaga gctcatataa taaggccagg atatggtatt
    13681 gaatacgatt atgttttgcc cactcagctt cgagcgtcct tggagtgtcg agaaataccc
    13741 gggttatttt tagccggaca aatcaacggc acttcaggat atgaagaagc agcaggacag
    13801 ggaatagtag ccgggattaa cgctgcaagt tacgtcaaaa atcaacctcc tctaattctt
    13861 cggcgagacg aaagctacat tggagttatg gtcgatgact tagtgactaa aggagtagat
    13921 gaaccctacc ggctgcgcac tggaaaagta gagtatcgct tagtggtacg ccacagcaac
    13981 gctgacctcc gtctcagctt ttatgcttac gaacgaggaa taataagtaa aaaagagtat
    14041 gagcgagtgg aagaaaagag agataaaatc cagaaagaaa ttgagcgcct ttctcatctt
    14101 actattttcc ccactgctca attacaaact ttgctggctc aaaagggaac aaacccagtg
    14161 aaagaagcca caaaagctag ttttcttctt tctcgtccgg agattagcta ccgggattta
    14221 gccccttttg attggggaag gccctttttg gaaccccagg taattgaaga agtagaaata
    14281 gaaataaaat atcgaggcta cattgagcgg caaaaaaggg aaatagaaga atttaaaaaa
    14341 atagagaata aaattattcc tcctgacttt gacttctctt ctgtgcaggt gatttctcag
    14401 gaggcacggg aaaaactgca gaaaataaaa cctgccacct taggacaggc tgcccgggtg
    14461 gcaggagtta ctccttctga catcgttgct ctctatattt tgttagagaa gtaattaata
    14521 taattccagg taccgctcta actcccaggg atgaacagca ctttcaaatt cttcccattc
    14581 ttggtattta gcttctaaaa agaaatccaa aatatgggga gtgagagctt ctcgaactat
    14641 ggaatctttt tctaattctt ccaaggcctc tcgcaggttg cgggggagac tgcggatatt
    14701 ccattcctct cgctcctcac ggctcatgcg ataaaggttg acactgttgc agggcttacc
    14761 ggggtctaac tctttatcca ctccgtctat tcctgctctc aaaatggcag caaaagctaa
    14821 ataagggttg caggaagggt cagggttacg caattctgct ctggttttct ttccccgttg
    14881 aggaggaact cgtaccagag gactgcgatt ttgctctgcc caggctatgt aaaccggagc
    14941 ttcataacca ggtaccagac gcttatagga attaactaaa ggattagtta tagcagttag
    15001 agctggagcg tgctgtaaaa gaccggcaat aaatcctcga gcaattttac tcaaaccatc
    15061 aggcccctcc gggtcgtaaa ataggttttc ttcacctcga aacagagaaa ggtgagtgtg
    15121 catacctgaa cccggtactc cataaagagg tttaggcata aaagtagcgc ataggccttc
    15181 cttgagagct aaagttttta ctgccagctt tagcgttatt aaactatcgg caatttttaa
    15241 cgccttattg tattcaaaat caatctcatg ttgtgaagga gccacttcat ggtgagaggc
    15301 ttcaatatta aaacccagat cctcaagagc aatcaccata tcccggcgta aactttcctc
    15361 caggtccaga ggtagaaggt caaagtaact tcctctatca ctcaccagaa ttttctgatt
    15421 ttcatactta aagataaaaa attctgcttc tgtacctacc tcaaaatccc atcccctttt
    15481 ttctacttct tttaataccc tttttaaatt agtacgagag catccttcaa aaacactacc
    15541 atctgcccgc cggacatcgc agatgattcg ggctacagaa tcttcttccc agggacagat
    15601 gataaaagta tccgggtcag gaattaattt catgtccgat tcttctattc tcacaaaacc
    15661 ttgaatagaa gaaccgtcaa aattaactcc ttcctccagg gcagcaggca actttttgat
    15721 gggaatttcc acatttttag ctctgcctaa aacatctaca aattgcaaac ggacaaagtt
    15781 aatattcttt tcttgagctt ttttcaatac atcttgaaaa ttagaggaca caattttcac
    15841 ctaccaggtt ttcccagctt tctttctcta tttttctcca ggaggaaact cgctctcggg
    15901 tttgagccaa aaactcttcc cccattttta tctctctttc cacttcttga ggagaggtgc
    15961 taccttccga tatttttctt ctcaccgatt tttcttcatc tattatgtct ttccagtttt
    16021 gaggaaacag gtcttgatat tctccccagt cttctttttt caaatcccgg agatgctttc
    16081 cgcttgagga aagctttttc actattttcc ccacccaggt atgagcttgg cgaaaaggaa
    16141 ctcctcgctc taccagatat tcacagagat cagtagcggt taaaaatcct gtagatagag
    16201 cctttttcat gctttctcta tcaggaaaga tgttttgagc gagacgagca gctatgcgaa
    16261 gagaggaaag agcttctttt actgctcgga aaagaggagg tttatcctgt tgtaaatccc
    16321 gattataagt gagagggaga ccttttagag taatagctaa actaacccag gagctcacca
    16381 cttctccagt ttttccccgc agcagttctg ccacatcagg attttttttc tgaggcataa
    16441 tactcgagcc agtcgtgaaa gcatcatcaa ttttcaaaaa gtgaaaagct ggagaactcc
    16501 acaaaactat ctcttccccc caccgactga ggtgaagaaa gagaatagac aggtctccta
    16561 aaaattccag aataaaatcc cgatcggata cggcatccat actgttttcc tgcactcggg
    16621 gaaaagctaa aagtctggct acatattgtc tatcgatagg cagggaagtt cccgatagtg
    16681 ctcctgctcc cagggaaagc acatttactc tttttcgagt atcttctaat ctttctacat
    16741 ccctctcaag cataaaaaaa taagccagaa ggtgatggga aaaaagaact ggttgtgccg
    16801 gctgaagatg agtatagcca ggcataacta catctttatt ttctcgagcc ttttgcaata
    16861 aagcttcctg aagagaatta atcccttcta taacctcttc tattttttct cgtaaaaaaa
    16921 gtcgctcatc taaaactatc tgatcattac ggctacgggc agtatgaagt ttaccaccta
    16981 tctcttctcc tgttatttct cggaggcgaa tttccaccag actatgtata tcttcaaaga
    17041 gggaagggtc tattttccct tcttctatct cttcgaatat ctttcgtaaa cctcgcaata
    17101 tttctttact ttctttctct gatattattc ctactttctc cagcatctta cagtgagcgg
    17161 cgcttcccca caggtcatat ttatagagga gaaaatcttc cggaatagaa gtggaaaact
    17221 ccatcacttc ccggtctaaa tctttttcca tccgacttcc ccataagttg gtcatagctt
    17281 cttccttttc tccagagtag cctgtacccg ggtaggaaga ccccatattt taataaaacc
    17341 ttcgctagcc cggtggtcaa ataaatctcc ccggtcatag gtagctaatc cccaatcata
    17401 gagagaatac cccgattttc ttcccactac ttgcatgcta cctttataaa gtttaattct
    17461 caccactccc gtcatccggg gtaagaaaga cttcataaaa gcatccagag cttcccggag
    17521 tggcgaatac cagagaccct catacactaa ctgagcatat tcttcttcca ggtgaggctt
    17581 aaaatggatt acttctcggg gaagaaccag attttgaagg tcgcggtaag cttcaagaag
    17641 gattaaagct gccggacact cataaatttc ccgggacttt attcccacta atcgattttc
    17701 cacatggtct acccggccaa agccattttc accacccact ctatttaact cctctaccag
    17761 gctcaaaagg gagtaatatt ttcctcctaa gaggcgaggc actccctctt caaatccaat
    17821 ttccagatag gtaggttcca gaggagcgtc ttggggattc ttcgtccact caaatacttc
    17881 ttccggcggt tcaaccagag ggtcttctaa aactccgcac tcaatgcttc ttccccagat
    17941 attctggtca atgctataag gttgctctaa agtaacggga accggaatac cattagcatg
    18001 agcatactca atttcttctt ctcttgtcca tttccaaacc cgggcaggag ccactacttg
    18061 taaatcagga tttaaggcca ttactgaaac ttcaatgcgt acctggtcgt taccttttcc
    18121 cgtgcaacca tgggctatta tggttgctcc tttttcttct gctactttga ccaggtactt
    18181 ggcaatcaaa gggcgagaaa gagcagtagc taaaggatac cggccttcat aaattgcttg
    18241 agcccctaga gcaggtaaaa catactcatt caaaaattct tcccgggtat caaaaaccag
    18301 tgcctctttc gctccaatac tcagagcttt tttacgcact tcttctaagt ctttaccctg
    18361 gcccaggtcc agagtaagag cataaacctc agcattaaat tgctcttgca gccagcggat
    18421 agccactgag gtgtccaatc ctcctgagta agctaaaact actttctgtt gactcatttc
    18481 agatcctcct tttcataaac tatatccttt ctaaaacttt cttcagtgct tctactccct
    18541 catctatttc ttcttgagtg ataatcaaag ggggtaaaaa cctcactgta tcatcattgc
    18601 aggcattgac taaaactccc tctttgagca tctcttcagt aatctttttt gctttcccgg
    18661 gaacttcaaa acctaacatc aaacctctac ctcgaacttc tttaaccatc gggaaaatag
    18721 aagctaactt tttgagttgc tcttcaaaat atttcccctt ttctctcact gtagagagga
    18781 aactttcctt acttacctca tctaccacca cacatgctgc ccgagaacac accggatttc
    18841 ctccaaaagt acttccctgg tctcctcgat gaaccgaaga agatgtttcc tcgtcaacca
    18901 aaaccgctcc caggggcaga cctcctccta ggcccttagc taaagtaact atatcaggcc
    18961 tgacttcaaa actctcaaaa gcaaagaact ttcccactct tcccatgcca cattgcactt
    19021 cgtcaaaaat taaaaccagg tctttctcat cgcataattt tctcaggccc agtagaaact
    19081 ccttattgca gggataaatt cctccttccc cctgtactgg ctctactatt atggcacaca
    19141 cctcatcatc cagaaccttt tctacacttt ccaaattatt aagggtagca tattctattc
    19201 ccggagggaa ggggtctaag tccttatgaa acttttcttg agcggtgagc cccagagtca
    19261 aaagagttct tccatgaaaa gagcgataga aggaaataaa tttatagcct ttaccatatt
    19321 ttctcttacg ataaaaacgg gccattttca aggctacctc atttgcttct gcaccactat
    19381 tgactaaaaa tacctgcgaa aaaccggatt tcttcaccag acgctctgct aattccacct
    19441 gaggcacact ataatacaga ttagaagcgt ggagcagttt atctacttct tcttttacag
    19501 cgctcttaag gggaggaaaa gaataaccta aaatattgac tgcaatcccg gccatgaggt
    19561 ctaaatatct ttttccttct aaatcaaaaa cataacaccc ttctcctcgc tctaatacca
    19621 ctggcaaacg actataagtt cccaaaaagt agcgtgcttc cttctctatg atttctgaag
    19681 aagtcattta cttcaccttc cttatattac tcatttcttc aaagcaccac ttttgttcct
    19741 ttcatctttc ccccagccaa aaagaaggaa agattttgca tttcttttcc ctcgccaatg
    19801 aacacttcct ttactccacc ctgcagggct tcttcggcca tctctaattt ggggatcatc
    19861 cctcctctaa ttttcccctc ttttttaaga gtttcaattc cagctcgggg caaaacttcg
    19921 attagagaat ttttatcttc catatcctgt agaactccgg ggacatcgga aaagagaaac
    19981 agtttctctg cttgcaaagc tacggctagg cgagcagctg cccaatcagc gttcacgttt
    20041 aagctctctc cttctttatc agctgctacc ggagaaataa tgggcaaaaa tcctccctcc
    20101 cacaaaaccc gcaccagacg aggatttact ttctttattt cccctactct tcccagatct
    20161 acctttttac ccccggaggt gagataatac ttttctcctt caaacaaaaa gccatctctt
    20221 ccacttattc cgcaagcagg aactccctga cgatgaaaaa tacccaccag catcttattt
    20281 aaaaggccgc tcagaaccat ttcggttatg tccatatcct cgggaggggt aactcgtaaa
    20341 ccttccacaa actggctttc tctaccttct ttttctaaaa atttagtgat ttgagcgcct
    20401 cccccatgta ccagcgctaa ttttatcccc tcccttatca gctgggtaag tacttgaaaa
    20461 cagtcctcgt ttttaaaatc gatattttta cctccaattt ttaccactat ttccataatt
    20521 ttctcctccc gatttacagg gaataacagt taatcacaaa aaccatagca attcctcttt
    20581 ttagcacttt actctatcct cggggcaatc tcgtttttcc cggtgttatt tatattttta
    20641 gtctcttttt taatctttac gccgtaaggc cgttctcagc tataatgact atttatccgt
    20701 acataatcta aagataaatc acaagtccat actccaaatt ctccttctcc tattcccagg
    20761 tccacaataa ttctgagctc ttttttcttc atcgcctctt ggccttgctc tctccctact
    20821 cctttagctt tcatccctcc ttctactaaa gtaaccccat taatctctaa attaacttta
    20881 tgagggtcta tctctattcc gctgcgcccc agagcagcca tgattcttcc ccagttgggg
    20941 tcttctccaa aaagggcagt tttcactaga ggagattcgg caacagtaaa ggcacctctt
    21001 cgtgcttcct ctttgttttt tgctccctgt actattatct ccacaaattt agttgctcct
    21061 tctccatccc gggctaaatc tcgggatagc tcctgagcta gccacaaaag cgcttcccgg
    21121 aaaaggggga aaacttcgtc tttttcttga gcctctattc cgcttttgcc gttagccaga
    21181 attaatacca tatcattggt gcttgtgtct ccgtctactg tcacccgatt aaagctctca
    21241 tccactactt ctttcaaaac ctgatttaaa gtcccagcat ctatacccag atcagtaact
    21301 ataaaagaca gcatagtggc caaatttggt ttgatcattc ctgagccttt agctattccc
    21361 cccaggtgca cagttttacc tttccaggaa aaagaaagag ctctttcttt ggggaaagtg
    21421 tcggtagtca ttatagcttt aactgcttct ctacttgatt tttttaccgg cagtatccgg
    21481 gataattctt ctatcccttt ttctatttta gggagaggca aaaattctcc aatcaccccg
    21541 gtagaagcta ctgccacgag gtgagagtct actttaaaac aggaagcagc aaagtcggcc
    21601 atttttcttg catcttctat tcctcgctca ccggtacagg cattggctac tccactattg
    21661 gctaccacca gttgcagctt tctttctttt aacaaatgtt cccgggtaac cagaaccggt
    21721 gctgctgaaa atcggttgct ggtaaaaact cctgctgccg ttgccggcgt atcagataaa
    21781 atagccgcca ggtcattttt ttctttcttt attccgcaat ttattccata ggaagaaaac
    21841 cctgaaacat tatccaatcc accttctaaa attttccagc tctccatctc tacctcctta
    21901 aggatataat acgtcctctg gtaaaccttc tttttctcct cttccaaaca tcagatttaa
    21961 attctgcacg gcctgcccgg ccgctccttt aacaagatta tccagggcag aaataactat
    22021 cagctggttc tctcccactc ttttaagccc caggtcaata aagttagaac ccaccaccca
    22081 gcgtagctca ggaaagcgat ggagggggaa aattcgcacc cagggtttcc ttccccagta
    22141 atctttccat atggataata gctgttcctc tttcatatct ttttccaggg tgagataaat
    22201 agtggttaaa atccctcttt tcaaaggcaa aagatgaggg gtgaaaacga gattgacttc
    22261 ccctcctttt gccagttcca gttgctcttt tatctccggt tggtggcgat gttctcccac
    22321 tttataagct ttgaaatttt catccacttc acaaaaatgg ttatcttcgg tagccttttt
    22381 gcccgctccg gtcactcctg atttactgtc cactattata gaaggtgaaa ctaccccctc
    22441 ttttaaaaag ggataaagag gaataatcac cgaagtagga taacaaccgg gattggctac
    22501 cagtcgcgcc tcttttaagc tctcttcata aatttctgga ataccatata ttgcttcccg
    22561 caaaagatga ggaacacggt gttctaattg ataatatttc tggaatgctt ctaggttttt
    22621 aagacgaaaa tcagccccta ggtcaataac ccgtattcct ttttgtaaaa gagaaggaac
    22681 ataatccata gccactcctg aagggagacc cagaaaaatt acctctattc cttctaattt
    22741 tttcacttcc tgtttttgca aagttaagct acttcttccc cgccaggccg ggcaataatc
    22801 ttctaatgtc atacctggaa aactctcgct acccacccag caaacttcta caccgggatg
    22861 gtgggataaa atgcgcaaaa gctcaagacc agtgtatcca gtagcgccca ttactcctac
    22921 atgtatcatc gttattcctc cattctaccc aataaaaaaa cctcgccttt agacgaggtt
    22981 tttaaagacg gacaggggtg caatttggtg cacggtgcca ccctgctata ctctttgctt
    23041 taacggcttt aaccggacct cctacttaga gaaaaactct tttcttcgga gtcagctcca
    23101 gggctctatc ttccttagcg tcacggggaa gctcacacct tgccttcctc gctgaaccat
    23161 aacgctaatc tcttcccttt cattgctttg agaaatattt aagcattttc tggtaattaa
    23221 atcaagtagg ttctgactat attgtgcttc cactttgaaa aattaaattt gctataatat
    23281 gacaaaacta atttttaggt gctaccttct t
//
LOCUS       DAOSVE010000020        22816 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_1473_length_22816_cov_73.115329, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000020 DAOSVE010000000
VERSION     DAOSVE010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 22816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22816
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1473_length_22816_cov_73.115329"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            419..2425
                     /gene="malQ"
                     /locus_tag="PK016_02965"
     CDS             419..2425
                     /gene="malQ"
                     /locus_tag="PK016_02965"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HXL02065.1"
                     /translation="MKNWEEKVKELSHLSGIQLYYYDTKGKKIEASLSTLLSLLQVMG
                     VEDLSEKGLEEKIKEKEEEKNNPFPPVVVTSERKLCFGPLSPQKVVLMVKGEKGEEWR
                     EEFKVENHSSLEWNFPPSMEWGYYELTFLLGEKESAYSVFLVFSPGRCFLPSHKMWGI
                     QVPVFALTTEKSQGIGDWRDLLFVEDKLKKAGGEFVGVLPLHLTENRSPHGISPYLPL
                     DRLLWNPIYLPLEEVASFLGYEEGKKYLAEISLFFSSFSPEKEIDYDEVWALKQKFLR
                     QTFQLFWERRNEDPRFLELDSFFQEERIRRASFFYAFAERQGLNWRSWPSPYRYKERK
                     ALEDYIQAHNEEALYHAYLQWLMERFLSFGSSLGFDLPVGASRGGSECFLEEDCFAFQ
                     ASLGAPPDDFAPAGQNWGFPPPFPEKERKNGYRHFIALIRKNMQFARYLRIDHILGWQ
                     RLYWIPEGSEARDGAYVRNFLPDLLGIAALESMRNKVTIIGEDLGTVDPLLKENLREF
                     QVLSTRVFYFEKDEQGFPLAPSQYPHLSLATFNTHDLPPLSSFWEGEDILLREKAGMY
                     EKKEVETYLKERKEFQEKVLQKLSQWGLWEEGGNLWEALLRFLALTPSLIKVVGLNDV
                     LGDKRMINLPGTIDEYPNWRLRLSIRESDLEETLHQIGKIMKGG"
     gene            2430..3227
                     /gene="mutM"
                     /locus_tag="PK016_02970"
     CDS             2430..3227
                     /gene="mutM"
                     /locus_tag="PK016_02970"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HXL02066.1"
                     /translation="MPELPEVEVIKREIEEKVTGSRILFVRVEDSQFYLPPDMILGKK
                     IKKLERKGKNLFLILEDESSLLFHLGMTGRLLFSPEEELFPYLKILLGLSSGFLSLTD
                     QRKLGKVAFLTREEREEFLRQVGIDPLSSEYTKENFDKVLKGKRIRIKDFLLSQRYIS
                     GIGNIYASEILFRSSIHPEKRTNDLTAGEKKKLFITIPQVLEEAIAQNGTTIRNYVRS
                     GGEQGEFQNSLLVYGKEGERCPRCGGEIVRRKIASRSTYFCPGCQTN"
     gene            3652..4101
                     /locus_tag="PK016_02975"
     CDS             3652..4101
                     /locus_tag="PK016_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HXL02067.1"
                     /translation="MEKILEVGMHLILDGYCQDSLIIGNLSEVHKFLDKFPEEMGMTK
                     VLPPYVFQYQSSSPRDSGISGIVIIAESHISIHTFPAQNYLSVDIFSCKPFDAEEAIQ
                     RLTDYFNLVEYSHQLFDRGIEYPSDLNLSLPLVIEERLKNLEKMVHT"
     gene            complement(4159..4512)
                     /locus_tag="PK016_02980"
     CDS             complement(4159..4512)
                     /locus_tag="PK016_02980"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HXL02068.1"
                     /translation="MEEKEKEEFRAQLLELSKRIKSILKREIDATRDYNPLSESRDEA
                     DLGSATADLALQMSSQKTLLEMLELVEKALKKIESGNYGLCELCKREISAQRLKMVPY
                     ALYCVECQSRMENKK"
     gene            complement(4765..5760)
                     /locus_tag="PK016_02985"
     CDS             complement(4765..5760)
                     /locus_tag="PK016_02985"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXL02069.1"
                     /translation="MRVKIFPPVGEVQKSGKGNDQGSRSKLEMSDRKGKPASFDAGKW
                     LLERVGIPFLLGVLESLALVLPPVLIKKFAFFLGNMFYRFSDLQLAKTQWKQIMKEND
                     DLEVNRHIQRVSRHFALSIMELMLLKKKGEQVLEDLVAEVEGEEFLKEALSQSRGVIL
                     VTAHLGNWELLGAWLGSHGYPVTTLVSSLPIPVVQRFMEEVRSAMKIELIERTNLLSA
                     RSILRQGKILGVLTDLRIPGGVVCDFLGREASSPATPAVFHLLLGSPIIPAFIIREGE
                     EMKHRIVFLPPLDWPLASSREENILRGTACCNQVVGEFVLRYPDQWNWFYQRWND"
     gene            complement(6136..6684)
                     /locus_tag="PK016_02990"
     CDS             complement(6136..6684)
                     /locus_tag="PK016_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HXL02070.1"
                     /translation="MKVSSLVRCALFIALYVAFAILFRFSSGVVPFSFLPFLVIMSGF
                     ILSPREAFFSLLGYIFLGLAGFPVFASPPFGGLTYLFNPTFGFLLGFLISAPFLSFIF
                     RRGKIHSLGKMILLSFGAVLLLYLPGLFYLWWSMTFLLGKPLSFYATLQIGFFPFIVF
                     DLLKAGGASFLAFHVLRRLSLF"
     gene            complement(6684..7448)
                     /locus_tag="PK016_02995"
     CDS             complement(6684..7448)
                     /locus_tag="PK016_02995"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HXL02071.1"
                     /translation="MFWQRKMEIPGLKKILSYETIDSTNNQAKLLAQDGYPEGTLVIA
                     EEQTKGRGRKGRSWISLPGKSLTFSLLLRPPQNTSLALLCALSCAWALEEKGFYVLLR
                     WPNDILLGDKKVGGILVESSFSGDDLLYVIMGVGINVNETEEDFPPSLRERATSLFLA
                     SSCQWQRGEILKSFLSFFFPAYFAWKEKGDFSPWVKDYEKRSSLLGKEVVVRWGKETV
                     QGIARGIKEDGSLVLENCGGETLISWGEATLHEDQK"
     gene            complement(7411..8511)
                     /locus_tag="PK016_03000"
     CDS             complement(7411..8511)
                     /locus_tag="PK016_03000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HXL02072.1"
                     /translation="MVGTNPSPLSPPRYDPNRLKRQVLIPVFISLAAIVFIFSFSSLR
                     GISLDLSSFRFFPLGISLVLIVCAWLIDSLRLFLTVRAWGKKIHFFHAFQAMLSNYFL
                     SSITPFSTGGSPAQAYILSRRGLTWGEAGSLVTVCGILYQVGLLTLLFVFIFVFHIGV
                     RLEGVLLHLLYSFAIFYSVVLSLLFFFLYRPSLLYRLTNWGISWVKKRFKRVKFSEEA
                     VREWIEEFFTDFQRGFTILFLRKPQYLVWNIICYMFNYWFIFSIAYFVLLSLGVKPSY
                     LEVVGAQIPLYYIFSFIPSPGASGGVELTLASVFLRFSGARRLGMFILLWRLITYYLP
                     MVVGGALFFQILGEGNNNNHNVLAEKNGNSRA"
     gene            complement(8519..9481)
                     /gene="cbiB"
                     /locus_tag="PK016_03005"
     CDS             complement(8519..9481)
                     /gene="cbiB"
                     /locus_tag="PK016_03005"
                     /inference="COORDINATES: protein motif:HMM:NF015164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HXL02073.1"
                     /translation="MVNTLNLLMGFLLDFFLGDPGGRWHPVVGIGKLIEGLEKVLFPR
                     ERHFRREFALGFVVVGIMVGGLGVTYFFLRRFLYSYSLVLFSVVEAYLIFNFLSLRTL
                     LRRGEEVKQALEARDLPGARKNLKNLVGRDTDYLSEWEVVRGCVESLAENFNDGVIAP
                     LFYISLFGALGGLIYKVTNTLDSMLGYKDFRYFFFGFASARLDDIFNFLPARLSILFI
                     ALAASILGRWGRSALFVAFRDARKHKSPNSGWPESAMAGALGVRLGGVNYYGGKREET
                     AFLGDFVLDLTPERIGEALSIVRLSSFLALSFFVALSILMGRVW"
     gene            complement(9481..10077)
                     /locus_tag="PK016_03010"
     CDS             complement(9481..10077)
                     /locus_tag="PK016_03010"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXL02074.1"
                     /translation="MKKIYLLRHGDTDATEKGYFAGWTDVPLSLKGKKRIDKVREILK
                     GIEFERFFVSPLARARETIQLIAPSCNWEVREEIKERSFGSWEGRHWEDLEEEFPEEV
                     RKWKEAPFLFVPPGGESFQAVLERVTPFWEELLHRPEEKVLVVTHGGVIRSIFAHLLG
                     VGFATTSCFLFDPGVLVKIRGDHSFCQVVTILNTEVEP"
     gene            complement(10074..10955)
                     /locus_tag="PK016_03015"
     CDS             complement(10074..10955)
                     /locus_tag="PK016_03015"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02075.1"
                     /translation="MRKRAICPGSMGELVQGLREGREVLISLPIDRYSEMELIWGEEE
                     RERKKNTLWKSEKALQIALREWGVEHLAEQITFRRLRKIPEGKGLASSTADIAALLGA
                     LARLLGKPVREEEIARIALQVEPTDGVFFPELSIFDHLEGRIKLSLPLPRYLGVVVVE
                     LPGERETLAVNREKLRENWLRHRREVEEAFRLVERGLEEGDLSLIGRGATLSGQIMQE
                     VEKEDLWEKVVSLSREMGASGVNRAHTGKAFGILFDRRVYTPREMLSRVREALKKEEV
                     KIEVNAVISGGVRVVEL"
     gene            complement(10952..11719)
                     /locus_tag="PK016_03020"
     CDS             complement(10952..11719)
                     /locus_tag="PK016_03020"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HXL02076.1"
                     /translation="MATEIKRAGKEAIRRLKLSWSFLTIFPVNTGEYKEGDLSRASFF
                     FPVVGTVIGAANALLFYLLYFLTRDSLLSAFGVTFLGIFLTRGLHLDGVADSFDALFS
                     GEPQERKQEILKDSHHGTFGVVGILLLTGAKIFLLQGMESNPLLFLFLAPLGGRVGAL
                     EWGAFFHPFPRSKRGLGEEFIGRVPPFYFFFWLAVIEVVSLMLGGTMTLLKIALVFAI
                     IYPLGRKLSEVFGGLNGDLVGLGIEAIEILILIGGRF"
     gene            complement(11704..12243)
                     /gene="cobU"
                     /locus_tag="PK016_03025"
     CDS             complement(11704..12243)
                     /gene="cobU"
                     /locus_tag="PK016_03025"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HXL02077.1"
                     /translation="MPESWDKSILFLGGARSGKSRLALEWVEKSGLSPLYLATGVATD
                     SEMEERIIRHQRERSPEWKVEEVPSGLGKVLERDWSEWGIVLDCVTFLVNNVFWEEKD
                     AERAYERLCRELSGILEKRKREHFLLLLVSNEVGMGVVPEYPAGREFRDLQGRVNQWI
                     AHQVDEAYLVVAGIPWQLK"
     gene            complement(12224..13258)
                     /locus_tag="PK016_03030"
     CDS             complement(12224..13258)
                     /locus_tag="PK016_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXL02078.1"
                     /translation="MIVHGGRAIEWQRKSGRTCLDFSASLNPWGPPREVKEKWEELFY
                     YATVYPPLDYSFYRKELANIYNLSPSFILPTNGATAGIYLLAWELPGETVLILEPCFS
                     EYARSFSRAGKRVERVFFPASSFSLPPADIVVWGNPTNPWGVSPPPFLLDYLWEMSLK
                     KGTILLVDEAFQEFLEEKSSWVFRLPENPRLYIIRSLTKYFTLPGLRGGFIAAHPSSC
                     SFLERYLEPWSINSLLARVLEIIAQGDLASFRATTQENLVREKRFLEENLEGGIMKFF
                     PSQVNFYTLQLKRGKEFFSSFLEERGILVRSLSDFFGLDDSYFRLAVRTHEENEQIVE
                     VVREFARILG"
     gene            complement(13262..15682)
                     /gene="gyrA"
                     /locus_tag="PK016_03035"
     CDS             complement(13262..15682)
                     /gene="gyrA"
                     /locus_tag="PK016_03035"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HXL02079.1"
                     /translation="MEETGKIVPVDVNREMKDAYLSYAMSVIVGRALPDIRDGLKPVQ
                     RRILYAMHEMNLRHSEPYKKSARLVGDVLGKYHPHGDMAVYDALVRMAQNFTYRYPLV
                     DGHGNFGSIDGDPPAAMRYTEVRMSPIASEMLRDLEKETVDFIPNFDESLQEPMVLPA
                     AFPQLLANGASGIAVGMATNIPPHNLTELIDACLYLLDHPQASVDDLLEIIPGPDFPT
                     YGKVVGREGIRDYFREGKGKLVLRGEVEVEEIGRHKMALLIKELPYQVNKANLVENIA
                     HLIDEKKLSDVSEVRDESDREGIRIVLELKKGAQPQRITRYLYRHTALEVSFGVILLA
                     LVDGRPETLGMKEALLHFLEHRRQVILRRSAHDLREGKDRLHILEGFLRALDDIDRVI
                     QLIRSSSSVSEAKERLQKEMDFSEKQAQAILDLRLQRLVALEREKILTEYEELKKEIR
                     RLEEILEKEEALKGVIRKELEEIKEKYGDARRTRIVEEEGGEGEIEDVELYPEEDVVI
                     TLTRDGYIKRTSLDSYRRQGRGGKGVTGILTKEEDAVSQIVVSTTWHRLLLFTSSGRV
                     FQLLSRNLPDASRSSRGVHLVNLLPLEEGERVFTFLSLRDFGVGKHLFFVTSKGKVKK
                     VDLLEFVSITRRGLKAIELEEGDELASVFISGGKDHVLLATAQGYGITFSEENVRPMG
                     RGARGVRGINLRPGDRVVGAVPLEANKHLVLVSTGGWGKRLEHALLPRHRRGGKGIRI
                     MQINKKVGEVVGIALAEEKDEILLSSQEGILIRLAVANIPVQGRAARGVKLIRLNEGD
                     RLASFALS"
     gene            complement(15693..17600)
                     /gene="gyrB"
                     /locus_tag="PK016_03040"
     CDS             complement(15693..17600)
                     /gene="gyrB"
                     /locus_tag="PK016_03040"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011229521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HXL02080.1"
                     /translation="MVEKKNRDYGASDIQVLEGLEAVRKRPSMYIGNVGVEGLHHLVF
                     EVVDNSIDEAVMGFCSRIEVTILEDDSVRVVDDGRGIPVEIHEAVGKSAVEVVLTRLH
                     AGGKFGGTAYQISGGLHGVGVSVVNALSEWLEVEIHREGKVWRQRYERGKPQGELKEV
                     GVSEHTGTVIHFKPDPLIFPDTHFHGDIISQRLKELAFLNPGLTIVFRDKTQEQEKVY
                     HFEGGISALVSYLNRGKEVLHPDPIYFSGEKDGIKVEVALQYNDGYLENILSFANNIR
                     TDEGGAHVTGFKSALTRAIIDYAEKRGLLKDKEETPTGNDVREGLCAVINVLIPDPQF
                     EGQTKTKLGNPEARSLTEEVTFSSLSSIFEENPDVARPVVEKVMQTVRARLAAKKARE
                     LARKKDSLDSISLPGKLADCAEKNPEIAEIFIVEGDSAGGSAKQGRDRKFQAILPLRG
                     KILNVEKANIHRILSNQEIRNIAAALGCGLGFDFSREKLRYHKVIIMTDADVDGAHIR
                     TLLLTFFYRYMKPLVEGGNVYIALPPLYRVREGKNDYYAFDDEELEQVLRNLRGKKYT
                     IQRYKGLGEMNPEQLWTTTMNPETRTLKQVTVEDAIEAEEIFSILMGDAVEPRRDFIR
                     EHAREVVNLDI"
     gene            complement(17613..17924)
                     /locus_tag="PK016_03045"
     CDS             complement(17613..17924)
                     /locus_tag="PK016_03045"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HXL02081.1"
                     /translation="MGKKEVKPFFGKPTPLREALEDFLKKEGLFPQLHSFILLSRFGE
                     WFPQLADFCRPVRLQEGVLYLEVDDPLWTLEVKKCIPDILLRYKEEGLEVKKIKIQCR
                     Y"
     gene            complement(17925..19010)
                     /gene="recF"
                     /locus_tag="PK016_03050"
     CDS             complement(17925..19010)
                     /gene="recF"
                     /locus_tag="PK016_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889914.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HXL02082.1"
                     /translation="MYLEKIELVNWRNLEKVELSFPEKVLVLGGDNAQGKTNFLEALF
                     LLARGVSPRRARDEEMIRWGKEFSYLRAQIREENLHFTREVVVKKEGKKEWKRDGKRE
                     TRRTSLPLVGYFPQDQEIIEGPPQKRRDFLDEAISFLSPSYGGELREYEKLLYRRNFL
                     LREHSPSELLEIYGEKLISCGAKVVETRIRYLHQFVPYFQGFHRELSGEDVSLQVAYR
                     SSGYRWEEGIEEGLRKARERVNEEERERGMTLFGPHRDEIVFLLRGREARDFASQGEK
                     KRMAISLRLAETSMAESFYQKEVVILVDDIFSELDRKRRNLLWQKIKDGSQVFLTTTE
                     EEEVKELKGSYPLSFFRVEGGRIERGE"
     gene            complement(19013..20098)
                     /gene="dnaN"
                     /locus_tag="PK016_03055"
     CDS             complement(19013..20098)
                     /gene="dnaN"
                     /locus_tag="PK016_03055"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HXL02083.1"
                     /translation="MDTIIKTEEFRRVLGKVYRVVPSRPPYPALSGILLEAKNGFAKL
                     TATDLEVGITSYCPVEVKEEGSAVLPGKVLYNLVKSLDSSELEIISLPDGNSEIKGGK
                     SYYKLSSFPPDDFPLFPPPSTEISSISGEKLKEGLMKVYFTASKDENNLPLTGVLFHF
                     EEEGKLSLVASDDHLLSICSIETRKGGGAEYILPLKAIIEIIRLAEGEVKIFPGTGEI
                     LFQWANLSLFSRLIEGEYPNYKRVIPQSFITQVSFSKEEFRKSLERVSLVVSPESPIV
                     GLLIGEGKIEISSSGEVGIAKEEVEAEIKGEEIKIFFNARYLLEVLRAFDEEKATLEI
                     SGQVTPTRLKGEGESLQYILMPVEIKE"
     gene            complement(20278..21630)
                     /gene="dnaA"
                     /locus_tag="PK016_03060"
     CDS             complement(20278..21630)
                     /gene="dnaA"
                     /locus_tag="PK016_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HXL02084.1"
                     /translation="MKVSPEELEGLWIKTLSLLEKQISVPEYRAWVENIQPLSLLNQR
                     LTIAVPSELSKEKIEKKYVEAIRYAFQKAAGTNFSGLEIGLVVKPSSVKEEEILSFSS
                     VKEEEKLSLNPRYTFSTFIVGNSNKFAHAAAMAVAENPAHSYNPLFIYGGVGLGKTHL
                     MHAIGHHILSKDPRVRIVYASSEKFTNELINSIRDDQTEAFRHKYRHVDILLIDDIQF
                     LAGKERTQEEFFHTFNTLHEAFKQIVLTSDRPPKEIPTLEDRLRSRFEWGLIADIQPP
                     DLETRVAILSKKAELERLELPSDIIYFIAMNIPSNIRELEGALVRIKAFCDLNKITPT
                     LEVAQEVLKDLLPKQETSSIPISSIQKVVAEYFNIKPALMKAKKRTKEVAYPRQIAMY
                     LARELTNYSLPSIGEEFGGRDHTTVLYAWEKVKSDIDSNPQLFQEISGLIEKLKREKS
                     "
     gene            22001..>22816
                     /locus_tag="PK016_03065"
     CDS             22001..>22816
                     /locus_tag="PK016_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02085.1"
                     /translation="MQIKTKISLGFIVLTLAALAIGVIAIFAFWQINTSLEEAQSYNP
                     LLLVTSRLKDLISQNSSLVSSYFREENVENLNQIEEKFTEINNRFLLYLEALRLGTES
                     EDFEKQHLGAWEKESFPYSLSPLPEDSPLFSRVQELKNLQINYQARVNMVKTLWKQYL
                     TTQEGRNKKSLEMGEPYGTYGIIMQFFQLVNDSVKQLMDPLDSTYNLLFRYAVSGDPD
                     GHLGKNIDNSFASLQRGIENSQVFSDETRKNIVTQIEDWKNKWEGLKGLLFTEE"
BASE COUNT         6729 a         5460 c         4345 g         6282 t
ORIGIN      
        1 gaaccttcct cagtggattc ccgataaaaa atctcgggaa tgacgaaaag agaagctctc
       61 gacgggagag ctttacatct tttttccctc gcccttgaag ggagagggtt agggtgaggg
      121 taggctataa gggggagaaa aagtttcccc ccttatagcc ccctaaaaag acaaatacaa
      181 cacggaggga aaaacctctg tgtttgtcgt ttctgaatgt ttctatcagg aatccaaatt
      241 ataggggaaa aagactattt agattcccaa ttaaaaaatt tgggaatgac ggaatggaga
      301 tgtcctccct ttttcttctc ccggataaaa gtggaggact gcaaaagttt tatcagtaac
      361 tctaaaatat tctcaaccga gagataaagc gtgatatgat attataagga gaagaaatat
      421 gaaaaactgg gaagaaaaag taaaagaatt gtctcacctc tcaggaattc aactatatta
      481 ctatgatact aaaggtaaaa aaatagaagc ttccctttct accctccttt cccttttaca
      541 ggtaatgggg gtagaggatt tgtccgaaaa agggttagaa gagaaaataa aagaaaaaga
      601 ggaagaaaaa aataatcctt ttcctccagt tgtggtaacc agcgaaagaa aactctgctt
      661 tggccctctt tctcctcaga aagtggtttt aatggtgaaa ggtgaaaaag gtgaagaatg
      721 gagagaagag tttaaggtag aaaatcattc ttccttagag tggaattttc ctccttctat
      781 ggaatgggga tattatgagc ttacctttct tttaggagaa aaagagtcag cgtattctgt
      841 ctttttagtt ttctctcctg gcaggtgttt tcttccttcc cataaaatgt ggggtatcca
      901 ggttccggtt tttgctctta ctactgaaaa aagccagggt attggagact ggagagattt
      961 actgttcgta gaagacaagc taaaaaaagc gggaggagaa tttgtagggg ttttgcctct
     1021 ccatcttact gaaaatcgtt ctccccacgg cattagccct tatctacccc tggatcgatt
     1081 actttggaac cctatttatc ttcctcttga agaagtagca tcttttctgg gatacgaaga
     1141 agggaagaaa tatttagccg agatttctct ctttttctcc tctttttctc cggaaaaaga
     1201 gatagattat gatgaagtgt gggcactaaa gcagaaattc ttgcgtcaaa ccttccagct
     1261 attctgggaa agaagaaacg aagaccctcg ctttttagag ttagattctt tttttcaaga
     1321 agagagaata agacgggcgt ctttctttta tgctttcgcc gaaagacagg gtttaaactg
     1381 gagaagctgg ccctctcctt accgatataa agaaagaaaa gccctagagg attatatcca
     1441 ggctcataat gaagaagcac tataccatgc ctaccttcag tggttgatgg aaagattcct
     1501 ttctttcggc tcctctctgg ggttcgattt accagtgggg gcctcacgag gggggagtga
     1561 gtgtttctta gaagaggatt gctttgcctt ccaggcttct ctgggagctc cacctgatga
     1621 ctttgctccg gcagggcaaa actggggatt cccccctccc tttcccgaaa aggagaggaa
     1681 aaacggttac cggcatttta ttgctctcat ccgtaaaaac atgcaatttg ctcgttatct
     1741 ccggatagac catattttag gatggcagcg cctttactgg attcccgaag gtagcgaggc
     1801 tcgagacgga gcttatgtca ggaactttct tcctgactta ctgggaatcg cagctctaga
     1861 aagtatgcga aacaaagtca cgataattgg agaagacctg ggaaccgttg accccctttt
     1921 aaaagagaac ttgcgagagt ttcaggtgct ttctacccgg gtattttact ttgaaaaaga
     1981 tgaacagggt tttcctcttg ctccttctca atatccccat ctttccctgg ccacctttaa
     2041 cacccatgac cttcctcctt tatcttcctt ctgggaagga gaggatattt tgctccggga
     2101 aaaagcagga atgtatgaga aaaaggaagt cgaaacctat ttaaaagaaa ggaaggagtt
     2161 tcaagaaaaa gtgcttcaaa agctttccca gtggggatta tgggaagaag gaggaaattt
     2221 gtgggaggca ttgctgcgct ttctggctct aactccttct ttaatcaaag tggtggggtt
     2281 gaatgatgtt ttaggagata agagaatgat taatcttcca ggaactattg atgaataccc
     2341 taactggagg cttcgcctat ccattcggga aagcgacctt gaggagaccc tgcatcagat
     2401 agggaaaatc atgaaaggag ggtgacaata tgcctgaact tcctgaagta gaggtaataa
     2461 aaagggagat agaggaaaaa gttacaggaa gtcgcatcct ctttgtccgg gtggaagatt
     2521 ctcagttcta tcttcctcct gatatgattt tagggaagaa gataaaaaag ctggaacgaa
     2581 aagggaaaaa tttgttttta attctggaag atgaaagctc actgcttttc catttaggaa
     2641 tgacaggaag gttgcttttt tctcccgagg aagaactctt tccgtacctc aaaattcttt
     2701 taggcttatc ttccggcttt ctctccttga ctgaccagcg gaaactggga aaggtagcgt
     2761 tcttaacccg ggaagaaagg gaagaatttt tgcgccaggt agggatagac cctttatctt
     2821 ctgagtatac caaggaaaat tttgataagg tcttaaaggg gaaaaggata aggattaaag
     2881 actttctcct ttctcagcgc tatatttctg gtataggtaa tatttatgct tcagaaattc
     2941 tctttcggag ctccatccat cccgagaaaa gaacaaacga tttaacagcg ggagaaaaga
     3001 aaaagctgtt tataaccatc cctcaagttt tagaggaggc cattgctcaa aatggaacca
     3061 ctattcggaa ttacgtccgt tccggaggag aacaaggaga gttccaaaat tcattgttag
     3121 tttatggaaa agaaggggag cgatgtcccc gctgcggggg agaaattgtg agaagaaaaa
     3181 ttgcttcccg cagtacttat ttctgtccag gttgccaaac aaactaaaaa atttgtttgc
     3241 cttattgaca tactcttgat taactatata ataggtaagg taataaaaat tgccaggggg
     3301 tggaaatcga gggctaactg aaggtgtttt cgcaccacca tattatttgt ccctccgcta
     3361 acacccggat agggaagtgc cccttcccta aggggggaag tgccccccgt attaatttgt
     3421 tttcggataa attcatccga aaggggagca cctaatgaaa agggttatca agctcaaatt
     3481 ctggttttta agaatttaat gaagtatggt cggggagatt acccgtttcc ctgaccattg
     3541 cttttgttaa aatatctatc cttgctcatg ttagcaagga tagatatttt attcttcggc
     3601 actttttagt caatagccgg tacaaaattc aaagtgaggg agggacagcc catggagaaa
     3661 atactagaag tggggatgca tctcatcctt gatggttatt gtcaggactc tctaatcatt
     3721 ggaaacctga gtgaagtaca taagtttctg gataagtttc ccgaggaaat gggtatgacc
     3781 aaggttcttc cgccttatgt tttccagtat cagagcagtt ctccccggga tagcgggata
     3841 tcaggtatag taataattgc cgaaagccac atcagcattc acacttttcc agctcaaaat
     3901 tacttaagtg tggatatatt ctcttgtaaa ccgtttgatg ctgaagaagc aatacaaagg
     3961 cttactgatt acttcaatct ggtggaatac agccatcaat tatttgatcg gggaatagaa
     4021 tatccttctg accttaattt aagtcttccc ctggttatag aggaaaggct caaaaacctg
     4081 gagaaaatgg ttcacacttg actcactact tatccaccag gaagggtgag cgtgcaccta
     4141 agaggctacg cgctcaccct attttttatt ctccatacgg gactgacatt ctacacaata
     4201 aagagcgtag ggaaccattt ttaaccgctg cgcagatatt tccctcttac acaattcaca
     4261 aagaccataa tttcccgatt ctatcttttt caaagctttc tctaccagct ctagcatctc
     4321 aagaagagtt ttctgggagg acatctggag agctaaatct gcagtggcac tccccaaatc
     4381 tgcttcgtct ctggactcac tcagaggatt ataatcccgg gtggcatcaa tttcccgctt
     4441 aagaattgac tttattcttt tgctcagctc tagaagttga gcgcgaaatt cttccttttc
     4501 tttttcttcc atacccttca tctccaagaa tataataacc catatttttt ctttatacaa
     4561 ccttatgtag gccctgggat agtgtcaacc cattttatct attattagtt tcggtctctc
     4621 tctccttaaa tttctctaca aaaagtttac ggcatacccg tctcgctggg tgaagttttg
     4681 gttaaaggat attgttttca gtagagcgaa gagaagctga aacgacaaaa ggttatccac
     4741 tttagtatga gatagcccta caggttaatc gttccatcgt tgataaaacc agttccactg
     4801 gtcggggtaa cgaagaacga actctcctac aacctggtta caacaagccg tccctcgaag
     4861 tatattttcc tcccgagaag aggcaagagg ccaatccaga ggaggaagaa aaacaattcg
     4921 atgcttcatt tcttctccct cacgaatgat aaaagctgga ataatcggac ttcccagcag
     4981 aagatgaaaa acggccgggg tagcaggaga agatgcttct cgacctaaaa aatcgcaaac
     5041 tactcctccc gggatacgca ggtcggttaa tactcccagt attttcccct gccgcaagat
     5101 tgagcgagct gaaagtaagt tggttcgttc aatcagctca attttcatag ctgatcttac
     5161 ttcttccata aatctctgaa ccaccggaat cgggagagag ctaaccagag tagtaacagg
     5221 gtaaccatga ctccccagcc aagcccccaa gagctcccag ttccccagat gagctgtcac
     5281 taaaatcact ccccgacttt gtgatagcgc ctcctttaaa aattcctctc cttccacttc
     5341 tgctaccaaa tcctcaagaa cttgctcccc tttctttttc aggagcatca actccataat
     5401 agataaggca aagtggcgag atacccgctg aatatggcgg ttaacctcta aatcgtcatt
     5461 ctccttcata atctgcttcc actgggtttt agccagctga agatcggaga aacgataaaa
     5521 catgtttccc agaaaaaagg caaatttttt gataagcacc ggaggaagaa ccaaagccag
     5581 actctccagc accccgagaa gaaaaggaat ccctactcgc tccaataacc actttcccgc
     5641 atcaaatgat gcgggcttcc ctttccgatc agacatttct aattttgatc ttgatccctg
     5701 gtcgtttccc ttgccactct tttgtacctc accaaccgga ggaaagattt tcactcgcat
     5761 aagtttcttc tcaccagtag ctctaccctg ctagttattg aattcctgtt cgatccagca
     5821 ctgtacttct ttttgcataa cctgagtgaa acgtcctcca atttcttaaa tttctcctca
     5881 gcatataaca gaaagccgct ggtcgagcca ggctcctcta ataagcctct tctgcttgcc
     5941 tcaaccagtc aagggcaggt gccaatctcc ttcaagtatt gtagcatagt tacgatttaa
     6001 tccccgcgct tccccttcag agataacaac aactgtaata acccacatga gaaacctaaa
     6061 aaaactgtgt aatatgttat tcctcttcac caaacttata gtcgtagatg ctgcccctcg
     6121 ctcaccattc gtaattcaaa aaagggagag gcgacgaaga acatggaaag cgaggaatga
     6181 agctcctcca gcttttaaaa ggtcgaacac aataaagggg aaaaatccta tctgtaaagt
     6241 ggcataaaaa gaaagaggtt tacccaaaag gaaggtcata ctccaccaca gataaaacag
     6301 cccgggaaga taaagaagca aaaccgctcc gaaggagaga agtatcattt tccccaaaga
     6361 atggatttta ccccggcgaa aaataaaaga aagaaaggga gcagatatta aaaaacccag
     6421 caaaaatcca aaagtaggat taaaaagata ggttaatcct ccaaaagggg gagaagcaaa
     6481 aactggaaaa cccgccaacc ccagaaaaat ataccccaga agagaaaaga aagcttcccg
     6541 gggggagagg ataaaaccac tcataataac taaaaaagga agaaagctga aaggcacgac
     6601 tcccgaagag aaacgaaaca aaatagcaaa agccacatat aaagcgataa atagagcaca
     6661 tcttactaga gaagagactt tcattatttc tgatcctcgt gaagagtagc ttctccccac
     6721 gaaatcaggg tttctcctcc acaattttcg agaacaagag aaccatcctc tttaatgccc
     6781 cgggctatac cctggacagt ctcttttccc cagcgtacca ccacttcttt acccagaaga
     6841 gaagagcgct tctcataatc ttttacccag ggagaaaagt cccccttttc cttccaagca
     6901 aaataagcag ggaagaaaaa ggaaagaaaa gacttcaaaa tttctcccct ttgccactgg
     6961 caagaagaag ccagaaaaag agaggtggct ctctcccgca aagaaggagg aaaatcttct
     7021 tcagtttcat ttacgtttat ccccaccccc atgatcacat aaagtaagtc atccccggag
     7081 aaagagctct ctaccagaat ccctcccact tttttatctc ccagaaggat atcgttaggc
     7141 cagcgaagaa gaacataaaa tcccttctct tctaacgccc aggcacaaga aagagcacaa
     7201 agaagagcta aagaggtatt ctgaggagga cgaaggagaa gtgaaaaagt gaggctcttt
     7261 cctggaaggg aaatccagct tcttcccttt cttcctctac ctttggtctg ttcttctgct
     7321 ataaccagcg ttccctcggg ataaccatcc tgagcaagga gcttagcctg gttgtttgta
     7381 gaatcaatgg tctcgtaact gagaattttt ttaagcccgg gaatttccat ttttctctgc
     7441 cagaacatta tggttattat tgtttccctc tcctaaaatc tgaaaaaaca aagcccctcc
     7501 caccaccatg ggaagataat aggttatgag tctccataac aagataaaca tccccagacg
     7561 tcgagcacca gaaaaccgca gaaaaaccga agccagagta agctccacac ccccactcgc
     7621 ccctggagag gggatgaagg agaaaatgta ataaagggga atctgagctc ctaccacttc
     7681 taaataggaa ggttttaccc ctaaagaaag gaggacaaaa taagcgatgg aaaaaataaa
     7741 ccaataattg aacatatagc agataatatt ccaaactaaa tactgaggtt ttcttaaaaa
     7801 aagaatggta aacccccgct ggaagtcagt gaaaaattcc tcaatccact ctctgactgc
     7861 ttcttcagag aattttactc gtttgaacct tttttttacc caggaaatcc cccagttagt
     7921 cagacgataa agaagagagg gacgatataa aaagaaaaaa agcaaggata agaccacact
     7981 ataaaatata gcaaaagaat aaagaaggtg aagcaacact ccttctaagc gaaccccgat
     8041 gtgaaaaaca aagataaaga cgaaaagcaa agtcaataag cccacctggt agagaatgcc
     8101 acaaaccgtt accagactcc ctgcctcacc ccatgttaac cctcgacgag ataaaatata
     8161 agcctgagcc ggactaccac cggtagaaaa aggagtgata gaggataaaa agtaattgga
     8221 aagcatagct tgaaaagcat ggaaaaaatg gatttttttc ccccatgccc gaacggtaag
     8281 aaaaagacga aggctatcga tgagccaggc acacacaatt aataccaggc ttatccctaa
     8341 agggaagaat cggaaagaag aaaggtcgag agaaatcccc cgcaaagacg aaaaagaaaa
     8401 aataaagaca attgctgcca ggcttataaa taccggaatc aatacctgcc ttttcagccg
     8461 attagggtca tagcggggag gagaaagagg ggaaggatta gttcctacca tgttccaatc
     8521 accataccct gcccattaaa atcgaaagcg ccacaaaaaa gctcaaagcc agaaaagaag
     8581 aaagacgcac aatcgataat gcctctccaa tccgctccgg agtgaggtct aaaacgaaat
     8641 ctcccagaaa agcagtttct tctcttttcc ccccgtagta attcactccc cccaatctca
     8701 cccctaaagc gccggccata gcactttctg gccatccaga gttgggactc ttgtgtttgc
     8761 gggcatctcg aaaggccaca aacaaggcac tccttcccca acgacccaga atagaggcag
     8821 caagagcaat aaaaagaata ctcaagcgag cgggaaggaa attaaatata tcatcaagcc
     8881 gagcagaggc aaatccaaag aaaaaatagc gaaaatcttt atagcccagc atagaatcca
     8941 gagtattagt tactttataa atcaaccctc ctaaggcacc aaaaagagaa atgtaaaaaa
     9001 gaggagctat caccccatcg ttaaagtttt ctgccagaga ttccacacac cctcttacca
     9061 cttcccattc tgagagatag tcagtatctc ttcctacgag gtttttcaag ttttttcgcg
     9121 ccccgggtaa atcccttgcc tccagagctt gctttacttc ttctccccga cgcaagagag
     9181 tacgcaaaga caaaaaatta aaaatgaggt aagcctctac caccgaaaag agaaccaaag
     9241 aataagaata gagaaagcgg cggaggaaaa agtaagtcac ccccaaccct cctaccatta
     9301 ttcccaccac cacaaaaccc agagcaaatt cccgccgaaa gtggcgttcc cgagggaaaa
     9361 gaaccttttc taggccttca attaatttac caatccccac caccggatgc catctccctc
     9421 ccgggtctcc cagaaaaaaa tccagaagaa agcccatgag gagattcaaa gtattaacca
     9481 ctaaggttcc acctcagtat ttaatatggt caccacctgg caaaaagaat ggtcacctct
     9541 tattttaacc agaaccccag ggtcaaagag aaaacaggag gtagtagcaa aacccactcc
     9601 cagaaggtgg gcaaatatag agcgaatgac tcccccatgg gtaaccacaa gcaccttttc
     9661 ttccggccgg tggaggagtt cttcccagaa aggagtcact cgctctaaaa cagcttgaaa
     9721 gctttctccc cccggaggaa caaaaagaaa aggcgcctct ttccactttc taacttcctc
     9781 gggaaattct tcttctaagt cttcccagtg ccttccctcc cagcttccaa aagagcgctc
     9841 tttaatttct tctctcacct cccagttaca ggagggagct attaattgga tggtctcccg
     9901 agctcgtgcc agagggctta caaaaaaccg ctcaaactct attcccttta gaatttctct
     9961 cactttatca attcttttct tgcctttcag agagagagga acatcggtcc agccagcaaa
    10021 ataacctttt tccgtagcat cagtatcacc atggcgcagc aaataaatct ttttcatagt
    10081 tccaccaccc tcactcctcc agaaataact gcgttaacct ctatttttac ttcttctttt
    10141 tttagagctt ctcttacccg agagagcatc tccctcgggg tatacactct tcggtcaaat
    10201 aagattccga aagcctttcc ggtgtgagcc cggtttactc cggaggcgcc catctcccga
    10261 gaaagagaaa ccaccttctc ccacaaatct tctttttcca cttcctgcat aatttggccg
    10321 cttaaagtag ctcctcgtcc tatcaaagag aggtctccct cttctaatcc ccgctctacg
    10381 aggcgaaaag cttcctctac ctcccggcgg tgtcttaacc agttctctcg caacttttct
    10441 ctatttaccg ccaaagtttc tctttctccc ggtaactcta ccaccaccac tcctaaatag
    10501 cgaggtagag gtaaagataa tttaattctc ccctctaagt ggtcaaaaat agagagctcg
    10561 gggaaaaaaa caccatcggt gggctccacc tgtagcgcta ttcttgcgat ttcttcttcc
    10621 cggaccggtt tcccaagaag tcgagctaag gcccccagaa gagcagcgat atcggcagtg
    10681 cttgaagcca gccccttccc ttccgggatc tttcgaaggc ggcgaaaagt gatttgctct
    10741 gctaagtgtt ccacacccca ctcccttaga gctatttgca acgccttttc gctcttccac
    10801 agagtgtttt tctttctctc cctttcttct tccccccaga ttaattccat ttcactatag
    10861 cggtcgatgg gcaaagatat aagcacctct cttccttctc gcaggccttg taccaattcc
    10921 cccatacttc ctggacaaat agctcttttt ctcaaaacct acctcctatc aaaattaaaa
    10981 tttctatcgc ttcgattcct aaacccacca ggtctccgtt caatcctcca aaaacttcac
    11041 tcaatttcct tcccagagga taaataatag caaagacaag ggcaattttc aaaagggtca
    11101 tcgttccccc taacattaaa ctcacaacct ctataacagc aagccagaag aaaaaataaa
    11161 agggaggaac tcgtcctata aattcttctc ccagacccct ttttgagcga gggaaaggat
    11221 ggaagaaagc tccccactcc agagccccca ccctccctcc caaaggggca agaaacaaaa
    11281 aaaggagagg attgctttcc attccctgca aaagaaaaat cttagctcct gtgagcaaca
    11341 aaattcctac cactccaaaa gtgccatgat gagagtcttt gagaatttct tgctttcgct
    11401 cctgcggctc accagaaaaa agagcatcaa aagaatcagc tactccatct aagtggagac
    11461 cccgggtgag gaaaatcccg aggaaagtta ctccaaaagc agaaaggagg gaatcccggg
    11521 tcagaaaata gagaagataa aaaagcagcg cattcgccgc tccaattact gttcccacta
    11581 caggaaagaa aaaactagct cgagaaaggt cgccttcttt atattctccg gtatttacag
    11641 ggaaaatggt gagaaagctc caggatagct ttaaccttcg tattgcttct ttgcctgctc
    11701 tttttatttc agttgccatg gtatccccgc tactactaaa tatgcttcat ccacctgatg
    11761 agctatccac tgattgactc ttccctgtaa gtctcgaaac tctcttcccg caggatactc
    11821 cggcactacc cccatcccca cttcattgct cactaaaagc aacaaaaaat gctctctctt
    11881 ccttttttcc aggatacccg atagctctct gcacaaacgc tcataagctc tttcggcgtc
    11941 tttctcttcc cagaaaacat tattcaccag gaaagtgacg caatccagca ctatccccca
    12001 ttcactccag tctctttcca gaactttccc cagaccagaa ggaacttctt ctactttcca
    12061 ctctggagaa cgctccctct ggtggcgaat aattcgctct tccatttctg agtcagtagc
    12121 cactccagtc gctaaatata agggagaaag accgcttttt tccacccact caagagctaa
    12181 tcgacttttc ccactccgtg ctcctcctaa aaaaaggatg cttttatccc aggattctgg
    12241 caaactccct caccacctct actatttgtt cattttcttc atgagttcgc accgccaggc
    12301 gaaaatagct atcatctaaa ccaaagaaat cagaaaggct cctcaccagt atccccctct
    12361 cctccaaaaa agaagaaaag aactctttac cccttttgag ctgcagggta taaaagttaa
    12421 cttgagaagg aaaaaatttc ataattcctc cttctaaatt ttcttccaga aatcgctttt
    12481 ctctgaccag gttctcctga gtggtagctc gaaaagaggc cagatccccc tgggcgataa
    12541 tttctaacac ccgggccaaa agggaattta tactccaggg ctccaaatat ctctccagga
    12601 aagaacaaga agaaggatgg gcagcaataa atcctccccg caaaccgggg agagtaaaat
    12661 atttggtaag agaacggatg atataaagac gagggttctc cggaaggcga aaaacccagg
    12721 aagacttctc ttctaaaaac tcctgaaaag cctcatccac aagaagaata gttccttttt
    12781 ttaagctcat ctcccagaga taatctaaaa gaaaaggagg agggctcacg ccccagggat
    12841 tagtggggtt tccccacact acaatatccg ccgggggaag ggaaaaggaa gaggccggga
    12901 aaaatactct ctctactctt tttccagcac gagaaaaact tcgagcatat tcagaaaaac
    12961 aaggctccag aatcaggact gtttctccag ggagctccca ggctaaaaga taaatccccg
    13021 ccgtcgctcc attggtgggc aggataaaag aaggagaaag attatagata ttggcgagct
    13081 cttttctata aaaagagtaa tcaagagggg gataaaccgt agcataatag aaaagctctt
    13141 cccacttttc ttttacctcc cggggaggac cccagggatt taagctggca gagaaatcca
    13201 aacaggttct accgcttttt ctctgccact ctatagctct tcctccatgc actatcatag
    13261 gttaactcag agcaaaagag gccagccggt ctccttcatt caggcgaatt aactttactc
    13321 cccgagcggc acgtccctgc accgggatat tagccaccgc gaggcgaatc aaaattccct
    13381 cttgggagga gagcaatatt tcatcctttt cctctgctag agcaataccc accacttcac
    13441 cgactttttt attgatttgc ataattctta tcccttttcc tccccggcga tgacgaggaa
    13501 gcaaagcatg ctccagcctt tttccccacc ctccagtaga aacgagaacc agatgtttgt
    13561 ttgcctccag gggaactgct cccaccactc gatccccggg gcgcaggttt attcctctca
    13621 ctcctctcgc tcctcgaccc ataggtctca cgttttcctc actgaaagtt attccatacc
    13681 cctgggcagt ggccagaagc acatggtctt tccctccgga gataaaaaca gaagccagct
    13741 catctccttc ttctaactct atggctttca gccctcggcg agtgatagag acaaattcca
    13801 gcaagtctac ttttttcact ttacctttgc tggtaacgaa gaaaaggtgt ttacccaccc
    13861 caaaatcacg caaagaaagg aaagtaaaca cccgctctcc ttcctctaga ggaaggaggt
    13921 tgacaaggtg aaccccccga gaagaacgag aagcatccgg caaattacga gagaggagct
    13981 gaaaaactct tcccgagctc gtgaagagca aaagccgatg ccaggtagta gagaccacta
    14041 tctgactaac ggcatcctct tctttggtca aaatacccgt aactcctttt cctcctcttc
    14101 cctgacggcg ataagaatct aaagaggttc ttttgatgta tccatcccgg gtaagagtta
    14161 tgactacatc ttcttcgggg tacagctcta cgtcttctat ctctccctct cctccttctt
    14221 cttccacaat gcgagttcgt cttgcgtcgc catatttttc tttgatttct tccagttcct
    14281 tccttataac ccccttcagg gcttcttctt tttctaaaat ctcttccaat ctccttatct
    14341 cttttttcaa ttcctcgtat tcggtaagga ttttttctct ctctaaagct accaagcgct
    14401 gcaagcgtaa gtccagtata gcctgtgcct gtttttcact gaagtccatc tccttctgca
    14461 gccgctcttt agcttcagaa acgctggaag aagaacggat gagctgaatc acccggtcaa
    14521 tgtcatccag agctctgaga aacccttcca gaatgtgaag ccggtccttc ccttctctta
    14581 agtcgtgagc gctccggcgt aaaattacct ggcgacgatg ttctaagaaa tggagtagag
    14641 cctctttcat tcccagagtt tccggtcgtc catccaccaa agcaagaagt atcaccccaa
    14701 aactaacttc gagcgccgta tgtctataga gataacgggt tatccgctga ggttgagctc
    14761 cttttttcaa ttccaaaact attcttatcc cttcccgatc cgattcatct cgaacttctg
    14821 aaacatcact aagttttttc tcatctatca aatgagcaat attctccact aaattggctt
    14881 tattcacctg atagggtaat tctttaatta aaagtgccat tttgtggcga cctatctctt
    14941 ctacttctac ttctcctcgc aagacgagct ttccttttcc ttctctaaag taatcacgaa
    15001 tcccttctct tcccaccact tttccataag taggaaaatc cggtccgggg atgatttcca
    15061 gaaggtcatc tactgaagcc tgaggatggt ctaaaaggta aaggcaggca tcaattaatt
    15121 ctgttaaatt gtggggagga atattggtag ccatgcccac tgcaatacct gaagcaccat
    15181 tggctaaaag ctgaggaaag gctgctggta aaaccatcgg ttcttgcagt gattcatcaa
    15241 aattgggaat aaagtctacc gtctcttttt ctaaatcccg aagcatctct gaagcaatag
    15301 gagacatcct cacctccgta taacgcatgg ccgcaggagg atctccatcg atggagccga
    15361 agttgccatg tccgtctacc agaggataac ggtaagtaaa attctgggcc attcgcacca
    15421 gggcatcata aactgccata tccccgtgag gatgatactt acccagcaca tctcccacca
    15481 gacgggcgct ttttttataa ggctcgctat gacgaagatt catctcatgc atggcataga
    15541 gaatgcgtct ctgcaccggt tttaacccat cccgtatatc aggaagggct cttcccacaa
    15601 taacgctcat agcatagctc aaataagcgt ccttcatttc tctatttaca tctactggaa
    15661 caatttttcc tgtttcttcc ataattatct ccttatatat ctaagtttac cacttcccgg
    15721 gcgtgttccc ggatgaagtc ccggcgaggc tctactgcat ctcccatcaa gatactgaaa
    15781 atttcctccg cttcgatggc gtcttctact gtaacctgtt tcaaggtgcg ggtttccggg
    15841 ttcatagtgg tagtccagag ttgttcgggg ttcatctccc ccagtccttt atagcgctgg
    15901 atggtatact ttttccctcg caagtttcgc aagacctgtt ctaactcttc atcatcaaaa
    15961 gcatagtaat cgtttttccc ctctcgcact cgataaagag ggggaagagc aatatagaca
    16021 tttcctcctt ctactaaggg tttcatatac cgatagaaaa aagtaaggag aagagtacga
    16081 atgtgagccc cgtctacatc agcatcggtc ataatgataa ctttatgata gcgcaatttt
    16141 tcccgggaaa aatcaaagcc cagaccgcaa cctaaagcag cggcaatatt tctgatttcc
    16201 tggttagaaa ggatacggtg aatattagct ttttctacgt ttaaaatctt accccggaga
    16261 ggtaaaatag cctgaaattt ccggtctcga ccctgtttgg ctgaacctcc tgccgaatca
    16321 ccttccacga tgaaaatctc tgctatctca ggatttttct ccgcacaatc agccaatttt
    16381 ccgggaagag agatagaatc cagactgtct tttttccgag ctaactcccg tgctttttta
    16441 gccgctaagc gagccctaac tgtctgcatc actttttcca ccacaggacg ggcaacgtcc
    16501 gggttttcct caaaaataga ggaaagagag gaaaaagtga cttcttccgt taaactcctg
    16561 gcctcggggt tacccagctt ggttttagtc tgcccttcaa attgagggtc aggaatcaaa
    16621 acattaatca ccgcacacaa cccctctcga acatcgtttc ccgtaggtgt ttcctctttg
    16681 tcttttagca accctcgctt ttctgcatag tcaatgattg cccgggtaag agcggactta
    16741 aatcccgtaa catgagctcc cccctcatca gtgcgaatat tattagcaaa agagaggata
    16801 ttctctaaat atccatcatt atattgtaga gctacctcta cctttattcc atccttttct
    16861 cccgaaaaat aaatggggtc aggatgcagt acttctttgc ctcggttgag ataagaaact
    16921 aaagcagaaa tccctccttc aaaatggtag accttttcct gttcttgcgt tttgtcacga
    16981 aaaacaatgg ttaacccggg gttcagaaaa gccaattctt taagccgttg agatattata
    17041 tccccatgga aatgggtatc ggggaaaatt aaagggtcag gcttaaaatg aatcacagtt
    17101 ccggtgtgct cactaactcc cacttctttt agctctccct gaggcttacc ccgttcatat
    17161 ctctgcctcc acacttttcc ctcccgatga atttccacct ccagccattc agaaagagca
    17221 ttaactactg atacccccac tccatgcagg cctccagaga tctgataagc tgttcctcca
    17281 aatttccctc cggcatgcag gcgagttaaa accacttcta ctgccgattt acccacggcc
    17341 tcgtgtattt ctaccggtat acctcgccca tcatcaacta ctctcacact atcatcctcg
    17401 agaatagtca cttcaatccg gctacagaag cccatcaccg cctcatcaat actattatct
    17461 accacttcaa acactaagtg atgtagcccc tccactccca cgttaccgat atacatactg
    17521 ggacgttttc tgaccgcctc taacccttcc agaacctgga tgtcacttgc tccataatct
    17581 cgattttttt tctctaccaa aactttatca cctcaatagc gacattgaat tttgattttt
    17641 tttacctcta atccctcctc cttatagcga aggagaatat ccggtataca ctttttcact
    17701 tctaaagtcc acagtgggtc atccacctcc aaataaagga ctccctcttg taatctgaca
    17761 ggacgacaaa aatcagctaa ctggggaaac cactccccaa aacgggaaag taaaataaaa
    17821 ctgtgtaact gagggaaaag cccctctttt tttaagaaat cttccagcgc ctctcttaga
    17881 ggagtaggct taccaaagaa aggtttaacc tcttttttcc ccatttattc ccctctttca
    17941 atccttcccc cctccacccg gaaaaaagag aggggataag aaccttttag ttctttcact
    18001 tcttcctctt cagtagtagt taagaatacc tgactcccgt ctttaatctt ctgccacaga
    18061 agatttctcc tttttctatc cagttcagag aaaatatcat ccaccagtat tactacttct
    18121 ttctgataaa aagattccgc catagaagtc tctgctaagc gtaaagaaat agccatcctt
    18181 tttttctctc cttgagaagc aaaatcccgg gcttctctac cccggaggag aaatactatt
    18241 tcgtctcgat gaggaccaaa aagagtcatt cctctttccc tttcttcttc atttactctt
    18301 tctctggctt tgcgcaaacc ctcttctatt ccttcttccc accggtagcc tgaagaacga
    18361 taggccacct gaagtgaaac atcctctcct gagagctccc gatgaaagcc ttgaaagtaa
    18421 ggaacgaact ggtggaggta tcggatacgg gtttctacca cttttgcacc acaactgatt
    18481 aatttctctc catatatttc aagaagttca gaaggagagt gctcccggag gaggaaatta
    18541 cggcgataga ggagtttttc atactctctg agctctccgc cataagaagg ggaaagaaaa
    18601 gaaatagcct catctagaaa atctctccgt ttctgaggag ggccttctat aatttcctgg
    18661 tcctgaggaa aataacccac gagaggaaga gacgttctcc gagtttctct ctttccatct
    18721 cttttccatt ccttttttcc ctcttttttt actactactt cccgggtaaa atggagattt
    18781 tcttcccgga tttgagctct caggtaagaa aattcttttc cccaccttat catttcctcg
    18841 tcccttgccc ggcggggaga taccccccga gctaagagaa ataaagcctc caaaaaatta
    18901 gtctttccct gagcattatc tccccctaaa accaggactt tctctggaaa agatagttct
    18961 actttctcta aatttctcca gttgaccagc tctattttct ctaaatacat tactactcct
    19021 tgatttctac cggcatcaga atatattgta gagattctcc ctctcctttt aaccttgtcg
    19081 gggtgacttg tccacttatc tccagagtcg ctttctcttc atcgaaggct cggagaacct
    19141 ctaagagata ccgagcatta aaaaatatct ttatctcttc tccttttatc tctgcttcta
    19201 cctcttcttt agctatcccc acttctccag aggaggaaat ctctattttt ccctccccaa
    19261 ttaagagccc tactattgga ctttcaggag aaacaaccaa tgatactctc tctaaggatt
    19321 ttctaaactc ttctttagaa aaagatacct gagttataaa gctctgcgga ataactcttt
    19381 tataattggg gtattctcct tcaatgagac gagaaaaaag ggagaggttt gcccactgaa
    19441 aaagaatttc tcctgtacca ggaaaaatct ttacttctcc ctcagcgagg cgaataattt
    19501 ctataatcgc tttcaaaggg agaatgtatt cagctcctcc tcctttcctt gtttctatgc
    19561 tacatatgct caagaggtgg tcatcacttg ctactaaact taacttcccc tcttcctcaa
    19621 aatggaataa cactcccgtc agaggaaggt tattctcatc ttttgaagcc gtaaaataaa
    19681 ctttcatcaa tccttctttg agtttctctc cagaaataga agaaatttca gtagaaggag
    19741 gaggaaaaag aggaaaatca tcaggaggaa agctagacaa tttataataa ctttttcctc
    19801 ctttaatttc agagttacca tcaggtagag aaataatctc taattctgaa ctatctaagc
    19861 tctttaccag attatataat actttacccg gtaaaactgc acttccctct tccttgactt
    19921 ctaccggaca gtatgaggta atacctacct ccaggtcagt agcagttagt ttcgcaaaac
    19981 cattcttcgc ttctagaagt atccctgata gagcaggata aggaggacga gaaggtacta
    20041 ctcgatatac tttacctagt actcttctaa actcttcagt tttaattatt gtatccatac
    20101 tttttctccc caagtttttc tctcctatta ctattatttt ttaaatataa aaaaactaat
    20161 agtagtaaca gagaaaattt attttgttaa gaagtaacta atacttatta ttcatttact
    20221 ttttcttctc ttaataacct gaacacaatc ccgggataag tggttggtaa actgtagtta
    20281 acttttctct ctctttaatt tttctattaa tccactgatt tcttgaaaaa gttgtggatt
    20341 actatctata tcactcttga ctttctccca ggcataaagg acagtagtat ggtcccttcc
    20401 tccaaattcc tcaccgatag agggaagaga atagttggtc agttctcggg ccaagtacat
    20461 agcaatttgc cgggggtaag ccacttcttt agtccttttc ttcgctttca tgagagcagg
    20521 tttaatatta aagtattctg ctaccacttt ctgaattgat gagataggaa ttgacgaagt
    20581 ttcttgctta ggtaaaaggt cctttagcac ttcctgagct acttccaaag tgggagttat
    20641 tttatttaag tcacagaaag ctttaattct tactaaagct ccttctaact ctctgatatt
    20701 ggaaggaata ttcatagcta taaaataaat aatatcggaa gggagctcga gcctctccaa
    20761 ctctgccttt ttactcaaaa tagcaacccg ggtttctaaa tctggaggtt gaatgtcagc
    20821 tattaacccc cactcaaaac gagaacgaag acgatcttct agggtaggaa tttctttagg
    20881 tggtctatca ctggttagga caatttgttt aaatgcctca tgaagagtgt taaaggtatg
    20941 gaaaaactct tcttgagttc tttcttttcc agctaagaac tgaatatcat cgatgaggag
    21001 gatgtctaca tggcgatatt tatggcgaaa agcttctgtc tggtcatctc taatagaatt
    21061 aattaattcg ttggtaaact tctctgaaga ggcataaact atccgtactc tagggtcctt
    21121 actcaaaata tgatggccta tagcatgcat aaggtgagtt ttacccagtc ctactccccc
    21181 gtaaataaac agagggttat aagagtgagc aggattttcg gccacagcca tagcggcagc
    21241 gtgagcaaat ttattactat ttcccactat aaaagtagaa aaagtataac gagggttaag
    21301 gctgagtttt tcttcttctt ttacagaaga aaaactcaag atttcttctt cttttacaga
    21361 agaaggtttt actaccaacc cgatttctaa tccggaaaaa ttcgttccag ctgctttttg
    21421 aaaagcataa cgaatagctt ctacgtattt cttttctatc ttttctttag ataactcact
    21481 cggtacggct atggtgagcc gctggttgag caaagataag ggctggatat tctccaccca
    21541 ggcacgatat tccggcacgg aaatttgttt ttctaataga gatagagttt ttatccacaa
    21601 accctctaac tcttctggag agactttcat ggccttgatc tcccattttt atttttttca
    21661 tcccccagtt ttccacaggt aattttacct gttagattaa attataacct cggaaataaa
    21721 ggaaaaaata tttttttctt tctcttccct tgtaaaagag gggatcattt ttttgctttt
    21781 taatttaacc ataaatacaa atttctggca agttattcac agcttatcaa cttttgtgga
    21841 taactgtaag aacaagaaaa attatttcct ttttaataga actgagttaa tattaaaagc
    21901 tgaaagtctc tataaagttt tttcttaaat tgccgatttg tattgttgaa aaagtatttt
    21961 ttaactgagc tagagaaagt ttgagaggga gggaaaaacc ttgcagatta aaacaaaaat
    22021 ttctttaggt tttattgttc ttactctggc agcattagct attggagtta tagctatctt
    22081 tgctttctgg cagattaata cttctcttga agaagcccag agttataacc ctttactttt
    22141 agttacttct cgattgaaag atttgatttc tcaaaatagt tctttagttt cttcttactt
    22201 ccgggaagag aatgtggaaa acctcaatca aatagaggaa aagtttactg aaatcaacaa
    22261 tcgcttcttg ctttatctcg aagcacttcg tctgggtact gaatcagaag attttgagaa
    22321 gcagcatctc ggagcatggg agaaagaaag cttcccctat tctctttctc ctttacctga
    22381 agatagccct cttttctccc gggtgcagga actaaaaaat ttgcagatca actatcaagc
    22441 ccgggtgaat atggtaaaaa ctctatggaa acaatatctt actacccaag aaggaaggaa
    22501 caaaaaatct ttggagatgg gtgagcccta cggaacttac ggcataataa tgcaattctt
    22561 ccagttggtg aatgactcag taaagcagtt gatggacccc ctagatagta cttataacct
    22621 tctttttcgt tatgcggtca gtggtgaccc tgatggacat ttaggaaaga atattgataa
    22681 ctcctttgcc agtttgcaaa gaggtataga aaattctcaa gttttctctg atgaaaccag
    22741 aaaaaatata gttacccaga tagaagattg gaaaaacaaa tgggaagggt taaagggtct
    22801 tttgtttact gaagaa
//
LOCUS       DAOSVE010000021        22278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_1512_length_22278_cov_71.558745, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000021 DAOSVE010000000
VERSION     DAOSVE010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 22278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22278
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1512_length_22278_cov_71.558745"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PK016_03070"
     CDS             <1..452
                     /locus_tag="PK016_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL02086.1"
                     /translation="LLNGRLDIFWDAFLHLIGPVLTLTYVQFAFILRITRSSMLEVLQ
                     KDYVRTARAKGLDERVVINKHARRNALIPVATVSGQTIIGLLAGAVIVETIFNRTGIG
                     SFMSLAAQQLDYASIMGGTLFFGFILVLGNLIVDISYAFIDPRVRLE"
     gene            456..1337
                     /locus_tag="PK016_03075"
     CDS             456..1337
                     /locus_tag="PK016_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL02087.1"
                     /translation="MNKETRRIFRRLFSNTSAILGFVLLIFFVVVAIFAPFIAPPPPN
                     KDPYIMPIVTWSSNPLPPSPEHPFGVAGKRDIFYGVVWGTRTAFRVGIVVTLISTVLG
                     LVVGSVAGYFGKLWDEVLMRITDIFMAIPFLVAAMVLTTILGTGLDKVMMAMTVFSWM
                     GPARLIRGNILQVKEEQFVLAAKALGVSDFSIILRHVLPNTIFPVLIQASMRMGSLVI
                     TAAALSFLGVGAPQGYADWGALLNYARNWMLGGQGEALKYWYMTVFPGLAMVLFVLAW
                     NLVGDTLRDAFDPRLRL"
     gene            1340..2347
                     /locus_tag="PK016_03080"
     CDS             1340..2347
                     /locus_tag="PK016_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL02088.1"
                     /translation="MEENNLLLQVQDLKVYFYTEEKEELRAVDGVSFYLKEGETIALV
                     GESGCGKSVTSLAILRLIDSDGKIQGGKIILEGRDLLSLSEKEMREIRGRDVSMIFQE
                     PSSALNPVYTIGDQIMEAIILHQNLSRPQARKAAEEVLRLVGIPEAHKRLDEYPHELS
                     GGMKQRAMIAMALSCHPKILIADEPTTSLDVTIQAQILELIEELQRQLNMGVILITHD
                     LGVVAGMAERVVVMYAGKVVEEGSRRDIFHNPSHPYTWGLLHSIPRLDVKQEKLAIIP
                     GVVPDPLNFPSGCRFRNRCPLEDEMCLTEPKRCEIEDQHYVYCHHYSQIEMLKTRGFE
                     Q"
     gene            2344..3336
                     /locus_tag="PK016_03085"
     CDS             2344..3336
                     /locus_tag="PK016_03085"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HXL02089.1"
                     /translation="MSDAPLLQVQNLKKYFPIKAGVFRRTVGWVKAVDGVSFEVLPGE
                     TLGIVGESGCGKSTLGMTIMRLYEPTAGNIVFQGQDISSLPASQLKSIRRNIQMIFQD
                     PFTSLDPRMHIGSIVEEGMKVHRIASSPEVRRRALDLLEKVGLGAEYFSRYPHEFSGG
                     QRQRIGIARALALNPRLVVADEPVSSLDVSVRSQILNLLKDLKREYQLTYIFISHDLA
                     VVRQICNRVMVMYLGKVVEIADGDVFFSSPSHPYSVALISAVPVPDPDYEVKRIILSG
                     DVPSPINPPPGCRFHTRCLVAQEVCSEVEPALEERGEGHWVACHFPGSLSWERV"
     gene            3404..3919
                     /gene="infC"
                     /locus_tag="PK016_03090"
     CDS             3404..3919
                     /gene="infC"
                     /locus_tag="PK016_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HXL02090.1"
                     /translation="MSIEVKYRVNHQIGAREVRLISPEGEQLGVVPLKKALEMAEEAG
                     LDLVEVAPDVHPPVCRIMDYGKFRYEKEKKIKLSRKKQKTSELKQLNLRPKIDQHDYQ
                     VKLKNAIRFLEDGDRVKIMVRFRGREAAFHERGEELLKKMIADLEEVGKVEQDIKYEG
                     PHLTVTLAPKK"
     gene            3932..4129
                     /gene="rpmI"
                     /locus_tag="PK016_03095"
     CDS             3932..4129
                     /gene="rpmI"
                     /locus_tag="PK016_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003871006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HXL02091.1"
                     /translation="MPKMKTHRGAAKRFKITATGKIKRGQAGKSHLLAHKDRDRKRRL
                     KKSVILDKSDEKRVRKLLARA"
     gene            4146..4499
                     /gene="rplT"
                     /locus_tag="PK016_03100"
     CDS             4146..4499
                     /gene="rplT"
                     /locus_tag="PK016_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HXL02092.1"
                     /translation="MPRAKSSVASRERRKKIMKSAKGYRGARSRSYRRAKEQVMRSQA
                     YAYRDRRNRKRDFRRLWIVRINAAAREEGMSYSKFMGALRKAGINLDRKNLSHLAIVD
                     QEAFQKLVEIAKSQL"
     gene            4585..5595
                     /locus_tag="PK016_03105"
     CDS             4585..5595
                     /locus_tag="PK016_03105"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HXL02093.1"
                     /translation="MQELEEYRESFQKEIEKVRTLSDWNSLKGKYIGKKSRWGEFLGK
                     LKELPAEEKRVWGKKINQLKKEMEELLEEKYQELLSIEEKLEIDITLPGKKGWSGSFH
                     PVFLTRDWILAIFQSLGFRIEEGPEVETEFYNFEALNIPPDHPARDDQDSFYLAPGYL
                     LRTHTSPAQIRVMEKLKPPFKVVVPGRCYRRDAFDATHSPVFHQVEGLVVARDISMGD
                     LKGTLETFVRRFFGEERKIRFRPGYFPFTEPSAEVDVSCGVCGGKGCRSCGYSGWLEI
                     LGAGLVHPQVFVHAGCSSQDVRGFAFGLGVERITMLKFGIKDIRWFWENDMQFLEQFN
                     IF"
     gene            5613..7964
                     /gene="pheT"
                     /locus_tag="PK016_03110"
     CDS             5613..7964
                     /gene="pheT"
                     /locus_tag="PK016_03110"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HXL02094.1"
                     /translation="MRISYNILKRFLPGLDIPQEELAPLLTEQGLMVERTWKVKELFP
                     FPLVVATLKRINKGILYSEGEIHYQGRVWKIKVNNWGMPEEGSKIILGMEGEVPHFLP
                     WGGEGKEEIIDFLITLPFPAEEGEEVSALLLGDDIVLDVEITANRGDCLSSLGLAREV
                     GAKLGLEWQIPPVSYEEADTPSDFSLENQAPDLCPYYTGKHLVKLSIRPSPWWVVKEL
                     SFLGLRPINNVVDFTNLVMMEIGQPLHAFDASVIKGRKVVVRRAEEGEKLITLDGIER
                     ELSSGMLVIADAERPIGLAGIMGGKESEITSFTSEVFLEAAIFDRVAVRKTAKALGLR
                     TEASARFERGVDPERVIYASGRVLQLLGKDGESAPGWFIAGTPPREEREVSFFPEEVE
                     KTLGCSIKREEMIKILVGLGFGIKSLPGEETIQVKVPSWRSDIYLPVDIAEEVGRIYG
                     YDKIISCLPQFSFDPGNQDSNTLKEEKLRRHLVDKGLKEIISFPLISPRLVELWGEKE
                     EQIEIKNPLSQEQSYLRPCLLLSALEVLKVNITRGRNNWGLFETGKVFRKGVDTPFQE
                     EKRLFIALTGYSLPPLWQRGGEVDIFLLKGLIEELLEIAGFSREEVRFENDGGLALEP
                     SCSFRVKISGKEAGRGGLLSSSWVRELGFWGKIYVAELSLDLLTAGEGKVFRLKEVVT
                     FPSIARDISILVDETTKWGEIEEMVKRTISREKMEVEEIEIFDLFKGENVPEGKKNFS
                     FRIIFRSPERTLNDEEVDKWVQKIKADLKEKRGLLLREELVNF"
     gene            7961..8497
                     /locus_tag="PK016_03115"
     CDS             7961..8497
                     /locus_tag="PK016_03115"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HXL02095.1"
                     /translation="MTVDWYSIIGLVILGINIIRSAFRGFSKEVLGLAGFLGGVVIGL
                     QYYPVIGGFFAHLFGRYYIWFSPLGFLLLFLPLVLASSWLGVRFRRVFQNLDITWIDS
                     ILGSFAGIAKGILWALILTLLLLNLSYFPVVNQLIYRSSFYLRLIQPLLVYLYEWVLK
                     IPHLQFLQGILQKGIGWV"
     gene            8488..10836
                     /locus_tag="PK016_03120"
     CDS             8488..10836
                     /locus_tag="PK016_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HXL02096.1"
                     /translation="MGIRLSPLVLETFDFDKIVERLVSFCSSSPTEELARSIAPFSPE
                     EVDLKTQEAREALDMIQFDGSLSLRGLPDLRSLFPRIEVVDASLSLENLMEVLRFFRL
                     TLETREEARERKIKEKYPSLSFYFDKIKDFRPLVKKMEKVISKEGYILDTASDRLWEL
                     RKKAKILQNRIGRELEEMLNSSQLAPLLQDRNYTQRRGRYVIPLKIQYRNAIDGLIID
                     YSSSGSTVFIEPARILELDNELELNRLQEEEEIARLLRELTSELRPFLEEIKETYDAL
                     IYLDLLQAKANLGKSWKGKVPEWGEKLVIRGGHHPLLGEKAVPFDLELSPDKSILVIS
                     GPNAGGKTVLLKSVALVVFLTFCGIPPPLEEGSSLPLYRYLFVDIGDHQDLENELSTF
                     TYRLSSLKKALQEAESPALFLIDELGGGTDPEEGSALAIAILQYLQKRGVTTLVTTHL
                     PAVKQYAFQEEGAIPASLAFDPRTLEPTYRLVVGEIGPSYGIHIAERVGIPAEIIQNA
                     ITIVDKREWEFNQLLLSLSQRREDLDRLAREYENKLRELREKEEELRHKEKELEKKRE
                     KIEKEEREEFADYLRQTQREISQLVGKLRKEERLDQEEYQKLKKRLGEEKKRWEELAK
                     NVMVSSPEENWQEGETVIVDFLKQKGKIISLDFKKKEAVVEIEGRKVKTSFSHLEKTE
                     KEEDDSPLFAAINSSPLPQVKNQIEIRALRWEEAWEKLEKYFDQVIIAGFHTVYIIHG
                     KGEGILREATHQFLHDNPYVESFRLGYPEEGGIGVTVVTLKY"
     gene            complement(10833..12743)
                     /locus_tag="PK016_03125"
     CDS             complement(10833..12743)
                     /locus_tag="PK016_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HXL02097.1"
                     /translation="MKRTKKSSPGWIFLSGLLAGGVIWLLLFAFVLSDLKITSNQLDD
                     FSPPLTTRLYDIKGRLLAELFTENREYVELSQVPDLIKKAFIASEDQNFYHHPGIDPA
                     GIIRAALQNLINMDIVEGASTITQQLSRNLFLSQQRTWKRKLQEMFLAFTLEKKYTKD
                     EILEAYLNQIYFGHGAYGIKAAAKIYFGKNLEELNLAEIAMLTGVARSPGNFSPYVDL
                     STAQRQQRRVLRRMQEMRFISQEQLEEALSTPIVLSGLEENRSLAPYFVDYLLKELQK
                     SYDEETIFGGGLKIYTTIDLDIQKIANQALKESGYQGAILCIEPQTGYIKAMVGGRDY
                     DESKFNRAVQAYRQPGSTFKPFIYSAALDSGFTPSTVLVDEPLTFPNGWKPQNYEKTF
                     NGPITLREALEKSVNIIGIKLLQTTGVDKVISYARKMGIKSDLRRDLSLALGTSEVNL
                     LELTNAYCTFANRGLVPEPIAIVRIENFSGEVLYESQRVLRRALSEDTAYIMARLLQG
                     VIERGTATAARIDRPAGGKTGSTEDFIDAWFVGFTPDLVCGIYLGNDDRTPLGPRKTG
                     GVIAAPLFSKVMKEAHKDIPIHDFYPPPGVREVKVCIKSGKLPSPGCKTVTVPFKAGT
                     EPEEWCRECTGN"
     gene            12820..13317
                     /locus_tag="PK016_03130"
     CDS             12820..13317
                     /locus_tag="PK016_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HXL02098.1"
                     /translation="MNKSQDQEKIKKISRSALIATLYVLLTILPPFYSFSYGPIQVRV
                     AECLTVLPFLFPETIWGLGLGCFLANLIGGLGFYDFFLGTLFTILAGYLTSRVPYSFL
                     APLPPILVNGFGVSIYLSFWFKVPYFYSVLYVMLGETIATYGIGYPLLTFLITKLKYH
                     RKEYR"
     gene            13320..14555
                     /gene="tyrS"
                     /locus_tag="PK016_03135"
     CDS             13320..14555
                     /gene="tyrS"
                     /locus_tag="PK016_03135"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HXL02099.1"
                     /translation="MSQEARLDKDMALIERGVEEIIGVEDLKRKLLRFYQTGKPLVVK
                     EGFDPSAPDIHLGHTVTLRKLRQFQDLGHQVVFLVGDFTGRIGDPTGKSETRRQLNEE
                     EVRRNAETYAQQVSKILDLQKITIEFNSKWLSKLTLVDLIEIASRITVARMLEREDFA
                     KRLKTGKPIGLHEFLYPVMQGYDSIALRADVELGGRDQRFNLLVGRDLQREFGQEPQV
                     VITMPLLEGTDGIEKMSKSLGNYIGVDEPPFTMFGKVMSIPDSLIEKYFLLLTDVSME
                     DIQRMKTEYEKGELHPRDWKKRLAFELVRMYHGEKEALLSQEDFERAFSHKEMPEEAQ
                     EIVISSSLLKEGKIWTLKLLQKTGIFSSNAELRRLIEQGGVYFNGERIQDVNTDLPVK
                     DGDYLKAGKKGFFRIKLED"
     gene            14757..16826
                     /locus_tag="PK016_03140"
     CDS             14757..16826
                     /locus_tag="PK016_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HXL02100.1"
                     /translation="MMKKYSPVSEKVPHFLHGADYNPDQWLKYPEVLKEDIRLMKLAN
                     CNVVSVGIFAWSALEPEEGKFNFEWLDEVIENLYKNGIYAILATPSGARPAWMSQKYP
                     EVLRVESNRVRNLHGKRHNHCYTSPVYREKVNIINTKMAERYSKHPGVIAWHVSNEYG
                     GECHCNLCQEAFRNWLKEKYKTLDNLNDAWWTAFWSHTYTDWSQIESPAPHGEDLIHG
                     LNLDWKRFVTYQTVDFFRNEIKPLKDINPKLPVTTNFMGIYEGLNYWEFSEYLDVISW
                     DSYPRWHSSDDDSDLASEISMIHDLNRSLKGGKPFMLMESTPSTTNWMSVSKLKRPGM
                     HLLSSIQAVAHGSDTVQYFQWRKSRGSSEKFHGAVVDHCGHENTRVFNDVKEVGETLK
                     KLDEIIGTGVNPEVAIIFDWENRWALKDAKGPRNAGIHYEETAKANYKPFWKNGVPVD
                     VINMDCDFSRYKVLVAPMLYMVKPGVGERIEKFVEDGGTFVATYWSGIVNETDLCFLG
                     GFPGPLRKVLGIWSEEIDALYDGEERLVAFNEGNELNLEGDYKAKELCDLVHLEGAVS
                     LATYQDDFYAGRPALTVNNFGKGKSYYIASRNEERFNDMFYGKLIKKLGLKKVINTQL
                     PHGVTAQMRTDGENDYVFIMNFTQEEKVIGLDDRDYFDLVADKMVKGEIKLPGYGVKI
                     LKRKRVL"
     gene            17014..19083
                     /locus_tag="PK016_03145"
     CDS             17014..19083
                     /locus_tag="PK016_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HXL02101.1"
                     /translation="MFPYSKEVDYQCWLNYGPLENTYFEEQYRDYLESIVVSENGLVV
                     DSIKNELCYFTKKLLNIDTVIAPVSAKNKFTIISAVGTSEFVDKFIKKEEASALKEEG
                     FLMKTVNDSDKSFILLASKSHRGLLYGTFKLIQHIQMGKSLDNLNLLENPGVSLRMIN
                     HWDNLSGSVERGYAGKSIFYSNNELVGNLDRIRDYARLLSSIGINSIVVNNVNVGEEE
                     TKLIDSKIDIVEKLNEIFRSYGIKVFLSINFASPAIIGGLETADPLNEEVASWWKERV
                     DFIYSRIPDFGGFLVKADSEGQPGPFSYHRSHVDGANMLARQLAPYRGFLIWRAFVYN
                     CYQDWRDEKTDRAKAAYDTFMPLDGKFLDNVILQIKNGPMDFQVREPVNPLFGGLQKT
                     NEILELQITQEYTGQQKHLCYLIPQWKEILEFDTFMFGEGSTVKKIISGSLFNSRNSG
                     MAGISNVGDDINWTGHTLAQANLYGFGRLAWSPDLKSEEITEEWIKCTFGNNEYFVEN
                     LTPMMLKSWNIYEKYTAPLGIGWMVNPGNHYGPNVDGYEYSRWGTYHRADHFGIGVDR
                     TLKSGTGYTAQYHKQNFEKFEHLDSCPDELLLFFHHVSYIHKLENNKTVIQHIYDTHF
                     EGVEDVQWLIDTWQQLKEFVDSKRYESVLSRLLEQKEHAEEWRDVINTYFYRKTMISD
                     EKSRKIF"
     gene            complement(20520..20810)
                     /locus_tag="PK016_03150"
     CDS             complement(20520..20810)
                     /locus_tag="PK016_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02102.1"
                     /translation="MSEMAKKEGKPKLIMSSEEWKNIVPSYMANNDVTGFGDEVCKLA
                     ENIDSIEEANKWLALAMNIWNTTPQPDRRGKSAIEIIRQQRGNRKSSPPSSR"
     gene            complement(20861..21460)
                     /locus_tag="PK016_03155"
     CDS             complement(20861..21460)
                     /locus_tag="PK016_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02103.1"
                     /translation="MIYMTLTNSTIERLVEWCYKSDIIANIRAQAREQFFGDDEVKAS
                     DYLADTGSFNGRERRFMGWFTFSFKLPDGRHPAELAAQAILKEPKLSLALDAIQKTRY
                     VTGVVTKIMNDRGFDMELEDEKFVINSPMLSRILRKGQLISAHILPNGQGRWLVGPGW
                     LTWPISPGPHMRPRLKTLFQPNPIEVERFLQEKQKHRKR"
     gene            complement(21457..21897)
                     /locus_tag="PK016_03160"
     CDS             complement(21457..21897)
                     /locus_tag="PK016_03160"
                     /inference="COORDINATES: protein motif:HMM:NF015629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HXL02104.1"
                     /translation="MDEKRSMKINLEDLCLAMENNSYDLDYYLDLETGEIIFISDYMD
                     DANLEELKDRIDENPDRYEPIPKAESYEDYDDMVDFISTVEDEHLVELLEVAIDGKGA
                     FRRFKDVLARYPEEKERWYHFKEERMKERAISWLEDIGISLQGE"
BASE COUNT         6549 a         3916 c         5140 g         6673 t
ORIGIN      
        1 ctcttctcaa tggaagactg gatattttct gggatgcttt tcttcatctt ataggcccgg
       61 ttttaacttt gacttatgtc caatttgctt ttattttgcg cattactcgc tcttctatgt
      121 tagaagtgtt gcagaaagat tatgttcgta ccgctcgagc taaaggattg gatgagcggg
      181 tggtaattaa taaacacgct cggcggaatg ctctcattcc tgtagccaca gtaagcgggc
      241 agactattat cggtctttta gccggagcag taattgtaga aactattttt aatcgtactg
      301 gtattggaag ttttatgtct ttagctgctc agcagctgga ttacgcctct attatggggg
      361 gaactctatt cttcgggttt atactggtgt taggaaatct gatagtggat atctcttacg
      421 cttttattga cccccgggtg aggttagagt agttgatgaa taaagaaact cggcgtatat
      481 tcaggagatt gttttccaat acttctgcta ttttaggttt tgttttgctt attttctttg
      541 tggtggtggc aatattcgct ccttttattg ctccacctcc tcctaataaa gatccttata
      601 ttatgcccat tgttacttgg agctctaatc cccttcctcc ttctcctgaa catccttttg
      661 gagtggcggg gaagcgagat atattttatg gagtggtatg gggaactcgc acagctttcc
      721 gggtgggaat cgttgtgact ttaatttcta ctgttttagg gcttgtagta gggtctgtgg
      781 ctggatattt tgggaaacta tgggatgaag ttttgatgag gatcaccgac atatttatgg
      841 ccattccttt tttggtggcg gctatggttt tgaccactat tctggggaca ggattagaca
      901 aggtaatgat ggctatgaca gtgtttagct ggatgggtcc tgcccgcctc attcgaggaa
      961 atatcctgca agttaaagaa gaacaattcg ttttggcagc taaagcttta ggagtcagtg
     1021 atttttctat cattcttcgt catgttttac ctaatactat ttttccggtt ttaatccaag
     1081 cttctatgcg tatggggtct ttggttatta ctgctgcggc tttgagtttt ttgggagtgg
     1141 gagctcctca gggttatgct gattggggag cactcttgaa ttatgctcga aactggatgt
     1201 tagggggaca gggagaagct cttaaatact ggtatatgac cgtgtttcct ggtttagcca
     1261 tggtgctttt tgtgttggcc tggaatttag taggagatac cctgcgagac gcttttgatc
     1321 ctcgtctgag gttataaata tggaagaaaa taatttactt ttacaagtcc aggatttaaa
     1381 agtttatttt tatactgaag agaaagaaga gctgagagca gtagatggag tgagttttta
     1441 tcttaaagaa ggggaaacta tagctttagt gggggagtca ggatgtggta aaagcgttac
     1501 ttctttagct attctgcggt tgattgattc tgatggaaag attcagggag ggaagataat
     1561 cttagaaggc agggatttgc tttctctttc agaaaaagag atgcgagaaa ttagaggtag
     1621 agatgtctct atgatttttc aggagccctc ttcagctctg aacccagtgt atactatagg
     1681 agaccagata atggaagcga taattttaca ccagaatctt tctcgacctc aagctcgaaa
     1741 agcagcagag gaagtactca ggctggtggg tattcccgaa gctcataaaa ggttagatga
     1801 atatcctcat gagctttcag gaggaatgaa gcagagagcc atgattgcca tggctttgtc
     1861 ttgccatcct aaaattttaa ttgctgatga gcctactact tctttggatg ttaccattca
     1921 ggctcagatt ctggaattaa ttgaagaatt gcagcgacag cttaatatgg gagtgattct
     1981 tatcactcat gatttaggag tggtagctgg tatggcagag agagtggtgg ttatgtatgc
     2041 tggaaaagta gtggaagaag gaagtcgccg ggatatattc cataacccct ctcatcctta
     2101 tacctgggga cttctgcatt ctatacctcg tctggatgtt aaacaggaaa aattggctat
     2161 cattcccggg gtagttcctg accctcttaa ttttccttct ggttgcaggt ttcgcaatag
     2221 atgcccctta gaagatgaaa tgtgtcttac tgagccaaaa cggtgtgaaa tagaagacca
     2281 gcattatgta tattgccatc attattctca gatagagatg ttaaaaactc ggggttttga
     2341 acaatgagcg atgctcccct attgcaagta cagaatttga aaaagtattt tcccattaaa
     2401 gcaggagttt tccgaagaac tgtaggatgg gttaaagcag tggatggggt gagttttgaa
     2461 gttttgcccg gagaaactct gggtatagtg ggagaatcag gttgtgggaa gagtactctg
     2521 ggaatgacta ttatgcggct ttatgagccc actgcaggaa acatagtttt tcagggacaa
     2581 gatatctctt ctttgccggc ttctcagcta aaaagcattc ggcgaaacat tcaaatgatt
     2641 ttccaagacc ctttcacctc tttggaccct cgcatgcata ttggttctat agtggaagaa
     2701 ggcatgaaag tgcatcgtat agcttcctct cctgaagttc ggcgcagagc actggattta
     2761 ctagaaaaag tgggtttagg agcggaatat ttttctcgtt atccccatga attttccgga
     2821 ggtcagaggc agagaattgg tattgcgaga gccttagctc tcaatcctcg tttagtggtg
     2881 gcagatgagc cggtttcttc gctagatgtt tcggttcgct ctcaaatttt aaacttatta
     2941 aaagacttaa aaagagaata tcaactgact tatattttta tttctcacga tctggcagtg
     3001 gtgaggcaga tctgcaaccg agttatggtt atgtatttag gaaaggtagt ggaaatagca
     3061 gacggagatg ttttcttttc ctctccttct catccttaca gtgtagcttt aatttctgct
     3121 gtgccggttc ccgatcccga ttatgaagtt aaacgaatta ttttgagtgg agatgtcccc
     3181 agtcctatta atcctccccc gggatgtcgt ttccataccc gctgtctggt ggctcaagag
     3241 gtgtgctccg aagtagagcc ggctctggag gaaagaggag aaggccactg ggtagcctgc
     3301 cactttccgg gtagcttatc ctgggaaaga gtttaaagta attgactaag atgaataaat
     3361 atggtatgtt tttgaaccat ccaagaaaga agctagggag gcgataagta tagaagtaaa
     3421 ataccgcgtc aaccatcaaa ttggagcgcg ggaagtacga ttgatttctc cggaaggaga
     3481 acagttagga gtcgttcctt taaaaaaagc cctggaaatg gctgaagagg ctggtttaga
     3541 cctggtagaa gtggctcctg atgttcaccc tcctgtatgc cggataatgg actatggaaa
     3601 gttcagatat gaaaaggaga aaaagattaa gctttcccgc aagaagcaga aaacttctga
     3661 actgaagcaa cttaacttaa ggcctaaaat tgatcagcat gattatcagg taaaacttaa
     3721 aaatgccatt cgcttcttag aagatggcga tcgggtaaaa ataatggtca ggtttcgagg
     3781 gagagaagca gcttttcatg agagaggaga agaactctta aagaaaatga tagctgactt
     3841 agaagaagta gggaaagtgg agcaggatat aaaatatgag gggccccatc ttacggttac
     3901 cttagcccct aagaagtgag gaggaatgaa catgcctaag atgaaaaccc atcgtggtgc
     3961 agcgaaacga tttaaaatta ccgctactgg taaaataaaa agaggacagg cagggaaaag
     4021 tcatcttctg gctcataaag atagagatag aaagaggaga cttaaaaaat ctgttatcct
     4081 ggataaatca gacgaaaaaa gagtgcgaaa attgcttgcc cgtgcttaaa aggagtgaat
     4141 gaactatgcc tagagcaaaa agtagtgtag cttctcgaga gagaagaaag aaaattatga
     4201 aatcagctaa aggttatcgg ggagctcgtt ctcgttctta ccgaagagct aaagagcaag
     4261 tcatgagatc ccaggcttat gcctatcgag accgaagaaa ccgcaagcga gatttccgcc
     4321 gtctatggat tgtgcgaata aatgctgcag caagagaaga aggaatgagt tatagtaagt
     4381 ttatgggagc tctccgtaaa gcaggaataa atctggaccg gaaaaacctc tcccatttag
     4441 ctattgttga ccaggaagcc tttcagaaac tggtagaaat agctaaatct cagctttaaa
     4501 tcctttgatt ttgctctttc atcctgaaaa attgggaact atcaatagtg ttccgattgt
     4561 ttttatggga atggaggaaa tattttgcag gaattagaag aataccggga aagttttcag
     4621 aaagagatag aaaaagttcg tacccttagt gactggaatt ctttaaaagg taaatatata
     4681 gggaaaaaaa gccggtgggg ggaattttta ggcaagctga aagaattgcc tgcggaggag
     4741 aaaagagtct ggggcaaaaa aattaaccag cttaagaaag aaatggaaga actgctggaa
     4801 gaaaaatacc aagagcttct ctccatcgag gaaaaattag agattgatat tactcttccg
     4861 ggaaagaaag ggtggtcagg ttcttttcat cctgttttcc ttacccggga ttggatttta
     4921 gctatttttc agagtctggg gtttcgaata gaagaagggc cagaggtaga aactgagttt
     4981 tacaattttg aagctctgaa cattcctcct gaccatcctg ctcgagatga ccaggattct
     5041 ttttatctgg ctcccggtta tttactccgc actcacactt ctcctgctca aatccgggtg
     5101 atggaaaaat taaaacctcc ttttaaagtg gtggttccgg gaaggtgtta tcgccgggat
     5161 gcctttgatg ctactcatag ccctgtattt catcaagtag agggcttagt ggtagctcga
     5221 gatatttcta tgggagacct gaagggtact ttagaaactt ttgtgcgtcg tttttttggc
     5281 gaggagagaa aaataagatt ccgcccggga tattttcctt ttaccgaacc tagtgcagaa
     5341 gtggatgttt cctgtggagt gtgtggggga aagggttgtc gttcctgtgg atatagtggg
     5401 tggttagaaa ttctgggagc tggtctagtt catcctcagg tttttgtcca cgctggttgt
     5461 agctctcaag atgtgcgagg ctttgccttt ggcctggggg tagagcgaat taccatgctt
     5521 aaattcggta taaaagatat aaggtggttc tgggaaaacg atatgcaatt tctggagcag
     5581 ttcaatattt tttaggcgag gccgatacct ttatgaggat ttcttataat attttaaaaa
     5641 ggtttttacc tggtttagat atccctcaag aagaattagc tcctcttctt accgagcaag
     5701 ggttaatggt ggagagaacc tggaaagtga aagaattatt tccctttcct ttagtggtag
     5761 ccactttaaa aagaataaat aaagggatac tttattccga aggggaaatt cattatcagg
     5821 gacgggtgtg gaaaataaaa gtaaataact gggggatgcc tgaggaagga agtaaaataa
     5881 tattaggcat ggaaggagaa gtgcctcatt ttttaccctg gggaggggag ggaaaagaag
     5941 agataataga ttttctgatt actcttcctt ttcccgcaga agaaggagaa gaagtctctg
     6001 ctcttctttt gggtgatgat atagttctgg atgtagaaat tacagctaat cggggagatt
     6061 gtctttcttc tctagggctg gcgcgtgagg tgggggcaaa attgggttta gagtggcaaa
     6121 ttcctccggt cagctatgaa gaagccgata ccccctccga tttcagtctg gaaaatcaag
     6181 cgccagacct ctgtccttat tacaccggga agcacctggt aaaactttct attcgacctt
     6241 ctccctggtg ggttgtgaaa gagctttctt ttttaggttt gagaccgatc aataatgtgg
     6301 tggattttac taacctggta atgatggaga ttggccagcc tttgcatgct tttgatgcct
     6361 cggtaattaa aggacgaaaa gtagtggtaa gaagggcaga agaaggagaa aaactgatca
     6421 cccttgatgg aatagaaaga gaattatctt cagggatgct ggtgatagct gatgctgagc
     6481 gtcccatagg cctcgcgggg ataatggggg gtaaagaaag tgaaattact tctttcactt
     6541 ccgaggtatt tttggaagca gcaatttttg atcgggtggc agttaggaaa acagctaaag
     6601 ctctgggtct gagaactgaa gcttctgctc gttttgagcg aggagtagac ccggaaagag
     6661 taatttatgc ttccggtcgg gtacttcagc ttttgggaaa agacggagaa tctgctccgg
     6721 gttggtttat agcgggcact cctcctcggg aagaaaggga agtatctttt tttccggagg
     6781 aggtagagaa aactctgggc tgttctataa aaagggaaga gatgataaaa attctggtcg
     6841 ggttaggttt tgggataaag tctcttcctg gagaagaaac aattcaggtt aaagttccca
     6901 gctggcggag tgatatttat cttcctgtgg acattgccga agaagtaggt cggatatatg
     6961 gttacgataa gattatctct tgtcttcctc agttttcttt tgaccctgga aaccaggata
     7021 gtaataccct gaaagaagaa aagctgagga gacacctggt agataaaggt ttgaaagaga
     7081 taataagctt ccccctgatt agtccccgat tagtggagtt atggggagaa aaggaagagc
     7141 agatagaaat aaaaaatcct ctttctcaag agcaatctta tcttcgcccc tgtcttcttt
     7201 tatcggcgct tgaggtttta aaagttaata tcacccgggg gagaaataac tggggattat
     7261 tcgaaacagg aaaggtattt cgaaagggag tagatactcc ttttcaggaa gagaaaaggc
     7321 tgtttatagc tttaaccggt tattcattac ctcctctgtg gcagagaggt ggagaagtag
     7381 acattttttt acttaagggt ctaatagagg agcttttgga gatagctggt tttagccggg
     7441 aagaagttcg ttttgaaaat gacggaggat tagctttaga accttcttgt tcttttcggg
     7501 taaagatttc cggaaaagag gcaggaagag gaggtctttt atcttcttcc tgggtcaggg
     7561 aattggggtt ctggggtaaa atatacgtgg cagagcttag cctggatttg ttaactgcag
     7621 gagagggaaa agttttccgg ctaaaagaag tagttacctt tccttctata gcccgggata
     7681 tttctattct ggtagatgag actacaaaat ggggagaaat agaggagatg gtgaagcgca
     7741 ctatctccag ggaaaaaatg gaagtagaag aaatagaaat ttttgacctt tttaaaggag
     7801 agaatgtccc tgagggtaag aaaaattttt ctttccggat tatttttcgc tccccggaga
     7861 ggactttaaa tgatgaagaa gtggataaat gggtgcaaaa aattaaagcg gacctcaaag
     7921 aaaaaagggg tctacttctg cgggaggaac tggtgaattt ttgaccgtag attggtattc
     7981 tatcataggc ctggtaatac tgggtatcaa tattataaga agtgctttcc ggggctttag
     8041 caaggaggtt ctgggattag ccggtttcct gggtggcgtg gtgataggtt tacaatacta
     8101 tcctgtaata ggtggttttt tcgctcattt gttcggtaga tactatatct ggttttctcc
     8161 tctgggtttt ttgctacttt ttctgcctct ggtattagct tcctcctggt tgggggtacg
     8221 cttccgaagg gtatttcaaa acctagatat aacctggata gattcaattc tgggctcgtt
     8281 tgcaggaata gccaaaggaa ttttatgggc tttaattctc actcttttac ttctcaatct
     8341 ttcctatttt ccggtggtaa accagcttat ttatcgttct tctttttatc ttcgactgat
     8401 tcaaccttta ttggtttatt tatatgaatg ggtattgaag attcctcatc ttcaatttct
     8461 acagggaatt ttgcaaaaag gaataggatg ggtataaggc tttctccttt agttctcgaa
     8521 actttcgact ttgataagat tgtagaacgt ctggtatctt tttgctcttc ttctcctacg
     8581 gaagagttag ctcgttctat tgctcctttt tcccctgaag aggtggatct taaaactcaa
     8641 gaagcccggg aagccctgga tatgattcaa tttgatgggt ctttgagtct tcgaggatta
     8701 cctgaccttc gctctctttt tccccggata gaggtggtgg atgcttccct tagtctggaa
     8761 aatttaatgg aggttttgcg ttttttccga ttgaccctgg aaacccggga agaggcgaga
     8821 gagcgaaaaa taaaagagaa atacccttct ctttcttttt actttgataa aataaaagat
     8881 ttccgtcctc tggtaaaaaa aatggagaaa gtgatttcta aagagggcta catcttagat
     8941 acagcaagtg ataggctctg ggaactgaga aaaaaagcaa aaattctcca gaatagaatt
     9001 ggtagagaat tagaagaaat gcttaatagt tctcaattag ctcctcttct tcaggataga
     9061 aattataccc agagaagggg acggtatgtc attccgttga aaattcaata tcgcaatgct
     9121 atagatggtt tgataataga ttattcttct agtggttcaa ctgttttcat tgagccagcc
     9181 agaattttgg aactggacaa cgaactggaa ttgaatcgac tgcaggaaga agaagaaatt
     9241 gctcgtttac tccgcgagct aacttctgaa cttcgccctt ttttagaaga gataaaggag
     9301 acttatgacg ctttaattta tctggattta ctgcaagcta aagccaattt aggtaaatca
     9361 tggaaaggga aagtccccga gtggggagaa aagttagtaa ttcgtggtgg tcatcatcct
     9421 ttactggggg aaaaggcagt cccttttgat ctggaattgt ctcctgataa atctattttg
     9481 gtgataagtg gaccgaatgc tggaggtaaa acagttcttt tgaaaagcgt ggctttggta
     9541 gtttttttga ctttctgtgg cattcctccg cctttagagg aaggttcgtc tcttccttta
     9601 taccgctacc tttttgtaga tattggtgac catcaagacc tggaaaacga attaagcact
     9661 tttacctatc gtctgagcag tttaaaaaaa gcattgcagg aagctgaaag tccggcatta
     9721 tttttaattg atgaattagg aggaggaaca gacccggagg aaggttctgc tttagccata
     9781 gccattcttc agtatcttca gaaaagaggg gttactactc tcgtgactac ccaccttcca
     9841 gcggtgaaac aatatgcttt ccaggaagag ggtgcaattc cggcatcttt agcttttgac
     9901 ccccggactt tagagcctac ttatcgattg gtagtgggag aaattggacc cagctatgga
     9961 attcatattg ccgaaagagt gggtattcct gcggagataa ttcaaaatgc cattactatt
    10021 gtagataaaa gggagtggga gtttaatcag ctcctccttt ccctctccca gcgccgggag
    10081 gacctggata ggttggcgag agaatatgaa aataagttaa gagagctaag ggaaaaagaa
    10141 gaagaattga gacacaaaga aaaagaactg gaaaagaaga gagagaaaat agaaaaagag
    10201 gaaagagaag agtttgctga ttatcttcgc caaactcaga gagagatatc tcagctggta
    10261 ggaaaattga gaaaagagga aagattggac caggaagaat atcaaaagct aaaaaagcgc
    10321 ttgggagaag agaaaaagcg gtgggaagaa ttagctaaaa atgtaatggt ttcctctcct
    10381 gaggaaaatt ggcaggaggg agaaacggtc atagtggatt ttctcaaaca aaaaggtaaa
    10441 atcatttctc tggatttcaa gaagaaagaa gcagtggtgg aaatagaagg aaggaaagtt
    10501 aaaacttctt tttctcacct ggagaagaca gaaaaagagg aggacgattc gcctttgttt
    10561 gctgctatta attcttcgcc tttacctcag gttaaaaacc aaatagaaat aagagcactc
    10621 aggtgggaag aggcctggga aaagttagaa aaatattttg accaagtaat tattgctgga
    10681 tttcatactg tttatatcat tcacggaaaa ggagagggaa ttctccgtga agctactcat
    10741 caatttttgc acgataaccc ctatgtggaa agcttccgtt tagggtatcc agaagaagga
    10801 ggaattggag ttaccgtagt cactcttaaa tattaattcc ccgtacactc cctacaccac
    10861 tcctcaggtt ccgttcctgc tttaaaagga acggtaactg tcttgcatcc aggagatggt
    10921 aactttccgc tttttataca tacctttact tctcttactc ccggaggagg gtaaaaatcg
    10981 tgaatcggaa tatctttatg agcctccttc attaccttag aaaacaaagg agcagctatt
    11041 actcctccag tttttcgtgg acctaggggg gtacggtcat cattacctaa gtatattccg
    11101 caaaccagat cgggagtgaa acctacaaac caggcatcga tgaaatcttc ggtacttccg
    11161 gttttacccc ctgctggccg gtctattctg gctgccgtgg ctgttcctcg ctctattacc
    11221 ccctgtagga ggcgagccat gatgtaggcc gtgtcttctg atagagctcg gcgtaaaact
    11281 ctttggctct catagagaac ttctccggaa aaattttcta tccgtacaat ggcaataggt
    11341 tcaggaacca ggcctctgtt ggcaaaagta caataagcat tggttaactc taaaagattc
    11401 acttctgaag ttcctaaagc cagggataag tctcgacgaa ggtcactctt aattcccatt
    11461 ttgcgagcat agcttattac tttatccacc ccggtagtct ggagaagttt tatcccgatt
    11521 atgtttaccg atttttctaa agcttctctt aaggtgatag ggccattaaa agttttttcg
    11581 tagttttgag gtttccaacc attggggaaa gttagcggct catctaccag aaccgtactg
    11641 ggtgtaaaac cgctatccag ggcagcagaa taaatgaagg gcttaaaagt ggagccgggc
    11701 tggcggtaag cctgtaccgc ccgattaaat ttactttcgt cataatctct tccccccacc
    11761 atagctttga tatatccggt ttgaggttca atacacagaa tagccccttg atatccgctt
    11821 tcttttaaag cctggttggc tattttctga atgtctaaat ctatggtggt gtatattttt
    11881 agtcccccac caaatatagt ttcttcatca taactttttt gcaattcttt aagaagataa
    11941 tctacaaaat aaggagcaag agagcggttt tcttccagcc cggagagaac tatgggggta
    12001 gaaagagcct cttctagctg ctcttgggaa ataaatctca tttcttgcat ccttctcagc
    12061 actcttcttt gctgtctctg agcggtggat aagtccacat agggagaaaa attgcctgga
    12121 gagcgagcca ctccggttag catggctatt tctgccagat tgagctcttc caaatttttt
    12181 ccaaaataaa ttttggctgc agcttttatc ccgtaggcgc catgcccaaa ataaatctgg
    12241 tttagataag cttctaatat ttcatcttta gtgtattttt tttctaaggt aaaagccaga
    12301 aacatttcct gtaatttcct tttccaggtt cgctgctggg aaagaaacaa attccgggaa
    12361 agctgttgag ttatagtgct tgctccttcc actatgtcca tatttattaa gttttgtaaa
    12421 gctgctctga tgatacctgc ggggtctatg ccggggtgat gataaaagtt ttggtcttca
    12481 ctggcaataa aagctttttt tattaaatcc gggacctggg aaagttcaac atactccctg
    12541 ttttcagtga agagctctgc taaaagtcgc cccttaatat catagagacg agtggtaaga
    12601 ggaggagaaa agtcatccag ctgattagaa gttattttta aatcactcaa aacaaaagca
    12661 aaaagtaaaa gccatattac ccctcctgct aaaagaccgg aaagaaaaat ccacccggga
    12721 gagctctttt ttgtcctttt catagctttc atttatttat ataatgtcga tataaaatcc
    12781 tttctttatt tagtatagca tgtataataa aatcgtttta tgaataaaag tcaagaccaa
    12841 gaaaaaatca aaaaaatctc tcgctcagct ttaatagcta ctctttatgt tctattaacc
    12901 attcttcccc ccttttattc tttttcttat ggtcccatcc aggtgagggt ggcggagtgt
    12961 ttgactgttc tgccttttct tttccctgaa accatctggg gtttgggttt aggttgtttt
    13021 ttagccaatt taatcggggg gttaggtttt tatgacttct ttttaggaac tctttttacc
    13081 atccttgctg gatacttaac atctcgagtt ccttactctt ttctggcacc tttgcctcct
    13141 attttagtga acggatttgg tgtatctatt tacctttctt tctggtttaa agttccctat
    13201 ttttactcgg tgctttatgt tatgctggga gaaactatag ctacttatgg tatcggatat
    13261 ccacttttga catttctaat aactaaatta aaatatcaca gaaaggagta tcgataaaat
    13321 tgtctcaaga agcaagatta gataaagaca tggctctcat tgagcgagga gtagaagaaa
    13381 tcattggagt cgaagacctg aaaaggaaat tattgcgttt ttaccagacc ggtaaacccc
    13441 tggtggtaaa agagggtttt gaccccagtg ctcctgatat tcatctggga cacactgtta
    13501 ctttaaggaa attacgtcaa tttcaagatt tagggcatca ggtagttttt ttagttggtg
    13561 attttacagg aagaattgga gaccccactg gtaaaagtga aactcgtcgg caattgaacg
    13621 aagaagaagt caggcgaaat gctgaaactt atgctcagca ggtttctaaa atcctcgatt
    13681 tacaaaaaat taccattgag ttcaacagta agtggttgag taaacttact cttgttgacc
    13741 tgattgagat tgcttctcgt atcaccgtag ctcgaatgct ggaacgagaa gattttgcta
    13801 aaaggttaaa aacgggaaag ccgataggat tgcatgagtt tctttacccg gtgatgcagg
    13861 gttacgactc tatcgctctt cgagctgatg tggagttagg agggagagac cagcgcttta
    13921 atcttttagt gggtcgggat ttacagagag aattcggtca ggagcctcag gtagtaataa
    13981 ccatgcctct tttggaagga actgatggga tagagaaaat gagcaaaagt ttaggtaact
    14041 acattggtgt agatgagcct ccttttacca tgtttggcaa agttatgtct atccctgact
    14101 ctctaattga aaaatatttc ctcctgttga ctgatgtttc gatggaagat atccagagaa
    14161 tgaaaactga atatgaaaaa ggtgaacttc atccccggga ttggaagaag agattagcct
    14221 ttgagctggt tcggatgtat cacggggaaa aagaagctct tctctcccag gaagatttcg
    14281 agcgggcttt cagtcataaa gagatgcctg aggaagctca agagatagta atttcctctt
    14341 ctcttcttaa agaggggaaa atctggactt tgaagcttct ccaaaaaacc ggtatttttt
    14401 cttctaatgc tgaactacgg agacttatcg agcagggtgg agtatacttt aatggagaaa
    14461 ggattcagga tgtgaatact gaccttccag taaaagatgg tgattatctg aaagcaggca
    14521 aaaaaggctt tttccggatt aaattagaag attgattttg cgagttagat ctcactaatt
    14581 attctactta atcttttttc gtgtttgtga gggaagcccg acgattctct tttggaattg
    14641 tactcagttt tgcgtgatga ttcttctgta tctggtacaa tgaaaaacgt aatacagcga
    14701 aggttaaaag gtgtttttat gctttcggaa agacttttta aaaaggagcg agaaaaatga
    14761 tgaaaaagta tagcccggta agtgaaaaag tccctcattt tttgcatgga gctgattaca
    14821 atcctgacca atggcttaaa tatccggaag ttttaaaaga agatataagg cttatgaagc
    14881 ttgcgaattg taacgtggta tcggtgggga tatttgcctg gagtgctctg gagcctgaag
    14941 aaggaaagtt taattttgaa tggctggatg aggttataga aaacctttat aaaaatggga
    15001 tatacgctat tttagcaaca cccagtggtg caagacctgc ctggatgtct cagaaatatc
    15061 ctgaagtttt aagagtagaa tctaacagag tgagaaatct tcatggtaaa cgtcataatc
    15121 actgttacac ctcacctgtt taccgagaaa aagtcaatat aataaacaca aagatggctg
    15181 agagatactc aaaacatcct ggagttatag cctggcatgt atctaacgag tatggaggag
    15241 aatgccattg caatctttgt caggaggctt tcaggaactg gttgaaggaa aaatataaaa
    15301 cccttgacaa tctaaatgat gcttggtgga ctgctttctg gagccatacg tacaccgatt
    15361 ggtcacagat agaatctcct gcaccccacg gagaagacct gattcatggt ctgaatttag
    15421 actggaaaag gtttgtaact taccagacag ttgatttttt caggaatgag ataaaaccgt
    15481 taaaagatat taacccgaaa ttacccgtga caacaaattt tatgggaata tatgaaggtt
    15541 tgaattactg ggaattttct gaatatttag atgtgatttc ctgggacagt tatccaagat
    15601 ggcatagttc agatgatgat tctgatttgg caagcgaaat aagcatgatt catgatttga
    15661 atcggtcctt aaaaggcgga aaaccgttca tgctcatgga gagtactcca agcacaacta
    15721 actggatgtc tgtcagcaaa ttgaaaaggc cggggatgca cttactgtca tctattcaag
    15781 cggttgcaca tggctcagat acggtgcaat attttcaatg gagaaaaagt agaggaagca
    15841 gtgaaaagtt tcatggtgct gttgtagacc attgtgggca tgaaaacacc agggtgttta
    15901 acgatgtgaa agaggtggga gaaacactta aaaaattgga tgaaataatt ggtacaggtg
    15961 taaatcctga ggtagcgata atctttgatt gggaaaatag atgggcgcta aaggatgcca
    16021 aaggaccgag gaatgcggga atacattatg aggagacagc taaagccaat tataaacctt
    16081 tctggaaaaa tggggtgccg gttgacgtca taaatatgga ctgtgatttt tcccggtata
    16141 aagtattagt tgctccaatg ctttatatgg ttaagccggg agtgggggaa agaattgaga
    16201 agtttgtaga agatggaggg acatttgttg ccacttactg gtcggggata gtgaatgaaa
    16261 cagacctctg ttttttaggc ggatttccag gtccactgag aaaggttctt ggtatctggt
    16321 ctgaagaaat tgacgctctt tatgatgggg aggagagact tgttgctttt aatgaaggaa
    16381 atgagttaaa tctggaagga gattataagg ccaaagaatt gtgcgattta gttcatttag
    16441 aaggcgcagt ttctttagca acataccagg atgacttcta tgccggccgg ccggctctaa
    16501 cagtaaataa ttttggaaaa gggaagtctt actatatagc ttccaggaat gaagaaagat
    16561 ttaatgatat gttttatggt aaattgataa aaaaactggg gctgaaaaaa gtgattaata
    16621 cccaactacc ccatggcgtt actgctcaga tgagaactga tggagaaaat gattacgttt
    16681 ttataatgaa cttcacccag gaagaaaaag taattggatt ggatgaccgg gactattttg
    16741 accttgttgc agataaaatg gttaaagggg aaataaaatt gcctggctat ggagttaaga
    16801 ttcttaaaag gaagcgggtt ttatagcacc ccgtaaaacc tgctaaacta ttttgtcttt
    16861 cttgggggtt ataagggggc agagcctcct tatagtttgc tattaggtat caggagagcc
    16921 tcgtgttgat agttaatcac aacaggtttg gtggttaact ttataattct gccttaaagg
    16981 taaggtaata gttatcaaaa ggagatttct aaaatgtttc cgtattccaa ggaagttgat
    17041 tatcaatgct ggttaaacta tgggccctta gaaaatactt attttgaaga acagtatagg
    17101 gactatcttg agagtattgt agtcagtgaa aacggcctgg tggttgattc tattaagaac
    17161 gagctttgtt attttactaa aaagctatta aacattgaca ctgtgatagc tcctgtctct
    17221 gcaaaaaata aatttactat tatatccgca gtggggacga gtgaattcgt tgataagttc
    17281 ataaaaaaag aagaggcttc ggcattaaag gaagaaggtt ttctgatgaa aacggtaaat
    17341 gactcagata aaagttttat ccttttagcc tcaaagtctc accggggttt attatatggc
    17401 acttttaaat tgatacaaca tatccaaatg ggaaaatctt tagataattt gaatctgctg
    17461 gaaaaccccg gtgtttccct aaggatgatt aatcattggg ataacttaag cggaagtgtg
    17521 gaaagaggat atgctggaaa gtccattttt tattctaaca acgaattggt tgggaatctg
    17581 gatagaataa gagattatgc aagattgctt tcatctatag gtatcaacag tatagtggtt
    17641 aataatgtca atgttgggga agaagaaaca aaattaatag atagcaaaat agatattgtg
    17701 gaaaagctca acgaaatttt tagaagttat ggcataaaag tttttttaag tataaatttt
    17761 gccagtcctg cgataattgg tggtttagag accgctgatc ctttgaacga agaggtggct
    17821 agctggtgga aagaaagggt tgactttatt tatagtcgta ttcctgactt tggtggtttc
    17881 ctggtgaaag ctgattcgga aggacaaccc ggaccgtttt cataccatag aagccatgta
    17941 gatggtgcaa atatgttggc caggcaactt gccccctatc gtgggtttct aatctggcgt
    18001 gcctttgttt acaattgtta tcaagattgg agagacgaaa aaacagatag agcgaaagct
    18061 gcatatgata catttatgcc tcttgacgga aaatttctgg ataacgtgat tctgcagata
    18121 aaaaatgggc cgatggactt tcaggttaga gaacccgtaa atcctctttt tggtggtctt
    18181 cagaaaacaa acgaaatact ggaactccaa atcacccaag aatatacagg gcaacaaaaa
    18241 catctttgtt atcttatccc tcagtggaag gaaattctgg aatttgatac ttttatgttc
    18301 ggagagggct ctacggttaa aaaaataatt tccggttctt tatttaattc ccgaaattct
    18361 ggaatggcag gtatatcaaa tgtcggagat gatatcaact ggacaggtca cactctggca
    18421 caggccaacc tttatggatt tggtcggctt gcctggtctc ctgatttaaa aagtgaggag
    18481 ataacagaag aatggataaa gtgtaccttt ggaaataatg aatattttgt ggagaattta
    18541 acgccgatga tgttaaaatc atggaacatc tatgaaaaat atactgcccc gttgggtata
    18601 ggctggatgg tgaaccccgg caaccattac ggtcctaatg ttgacggcta tgaatattct
    18661 agatggggaa cttatcatag agcagatcac tttggaatag gagtggatag aactctaaaa
    18721 tcagggaccg gttatactgc tcagtatcac aagcaaaatt ttgaaaaatt cgagcatctg
    18781 gattcctgtc cggatgaatt attgcttttt ttccaccatg tttcttatat tcataaatta
    18841 gaaaataata aaacggtaat tcaacatatc tatgatactc actttgaagg agttgaggat
    18901 gttcagtggt taatagatac ctggcaacag ttgaaagaat tcgttgactc caaaagatat
    18961 gaatcggttc tcagccggct tttagaacag aaagagcacg ccgaggaatg gcgagacgta
    19021 ataaatacat atttttatag aaaaacgatg atcagtgacg aaaaaagtag aaagatattt
    19081 tgaaaaaatg gacactgctt gctttcccgg gtaaagtatt tgtaccacca ctctggagaa
    19141 ggtaaagcga tgtcttaaat acagttcaat ctattctcgc tggaaattct ttgatgataa
    19201 aaatacaact tgttcgccat tatagttaac cgcaaaatcc attacaaaga ctatgagggg
    19261 gtaaaccccc tcataacccc cttcaaaaag gcaaaaaagt taaaaacaac tgagggagaa
    19321 agagtctccc tcagcgggac aaactccttt ataattccct tttttcgtca ccctggcaac
    19381 tttcgagtcg tttttagtct gcctctgaat gtatttatca gggggtctac agtatttaaa
    19441 agcatggatg tctgataaaa gcgctcaggc atgacagatt aggaagattc cccgctaaat
    19501 ttcaggcctg acgagtgatg aagattcccg attgaggatt tcgggaatga gagaaaggaa
    19561 agccccccga gggagagggt tagggtgagg gtaggttata agggggagaa aaagcttccc
    19621 cccccttata accccctgaa aagacgaaaa gacaaataca acacggaggg aaaaacctct
    19681 gtgtttgtca tttctgaatg tttctatcag gaatccaaat tataggggaa aaagactatt
    19741 tagattcccg ataaaagatt tcaggaataa cagagaggga agtcattcct gagtggttct
    19801 atcaggaatc taaattactt aagccgaagt tcaacctaaa atggttataa tgacttattt
    19861 tagtgggttt ctttcgaata agtagtgtag acttttcttc ctatttatgt ctaaatatct
    19921 cagataatgg ttgatttatc tgttattgcg atttaatata gtagtgtaaa ctaagaataa
    19981 tagggttaat gttcattaga gtaattcttt ggaaaaaagc aataaaactt agtattatta
    20041 ctaaattagc agaaaagata ctaatactcc ctaaattaaa aatagagaga tctttagtgt
    20101 agtatagact tttttatcta aaaaacctta aaatccactc actaagggct tttttccgat
    20161 atcgggttga acttcggctt aagggcaaaa acatctgaat tcccgatcaa agatttctgg
    20221 gataacggaa gagaactata agggggaaaa taaatcttcc ttatcaaccc ctggaaataa
    20281 aggaacaaaa aagcaaaagc ataaaagtaa aaaaccactg aagagtttat agtatccctt
    20341 actacatggc aatttcccat tttttgactt ccgatgtaaa ttacttttta aagttttagc
    20401 agtttaacca gttttgcggg atactataaa tattcctctg caattagaaa acactattct
    20461 tcggggagtg gctgagttgg aggagtgctc tttgccacct ttttatttta ttagctcgct
    20521 taccggctgc ttggtgggga agactttctg ttaccccttt gctggcgtat tatttcaata
    20581 gctgattttc ctctccgatc tggctgaggt gttgtattcc agatattcat agctagagct
    20641 agccacttat ttgcctcctc gatagagtca atattctccg ctaacttgca aacctcatca
    20701 ccaaacccgg taacatcgtt atttgccata taggatggga caatattttt ccattcctct
    20761 gatgacatga tgagtttagg ttttccttct ttcttagcca tctcagacat acgggctact
    20821 gctgcttcaa gcgtatcatc ttgaggatgt tcgacatttt ttacctcttc cggtgttttt
    20881 gtttttcctg aaggaatctc tctacttcta tggggttagg ctgaaagaga gtttttagcc
    20941 gtggacgcat atgtggccct ggagagatag gccaagttaa ccagcctgga cccaccaacc
    21001 atcggccttg cccgttgggc aaaatatggg cagagataag ctgtcctttc ctcaaaatac
    21061 gacttaacat cggactgttt attacgaact tctcatcttc cagctccata tcaaaacctc
    21121 ggtcattcat aattttggtg actacgccgg ttacatatct tgtcttctgg atagcgtcaa
    21181 gagcaagaga aagtttgggc tcttttagta tagcctgggc tgccaattca gccggatgtc
    21241 gcccatcagg caatttgaaa ctaaaggtaa accaacccat aaaccgcctc tcgcgaccgt
    21301 taaagctacc agtatcagcc agataatcac tcgcttttac ttcatcgtct ccgaaaaatt
    21361 gctctcgagc ctgggctcta atgttagcaa taatatcact tttataacac cattctacca
    21421 accgctctat tgtgctatta gtcaatgtca tataaatcac tccccctgca ggcttatgcc
    21481 tatatcctcg agccaggaaa ttgctctttc cttcattctc tcttctttaa aatgatacca
    21541 tctctccttt tcctcgggat agcgagccag aacatcttta aatcggcgga aggcaccttt
    21601 accgtctata gctacctcaa gcaactcgac aaggtgctca tcttccacag tggaaataaa
    21661 atctaccata tcgtcataat cctcataaga ctcagctttg ggtatcggtt cataacggtc
    21721 gggattctca tcaattctgt cctttagctc ttcaaggttt gcatcatcca tataatcaga
    21781 aataaatatt atctctcctg tttccaggtc taggtaataa tcaaggtcgt aggaattatt
    21841 ctccattgcc aaacaaaggt cttccagatt tattttcatg cttcgctttt catccatatt
    21901 ttcattttcc tcttttatca tctaagttat aagtcagttt atattataac cccacagaag
    21961 ttaaaagtaa tataaattta catcaatttc ccgctgaccg ctgctaaatc aggtattcaa
    22021 gcaactctat accatcgtga agagtaaaca gtatggtatt cctaaggtga gcgctactgg
    22081 ctccccttct gtttcagaaa gaaatgtgaa tgcaccgcct ggtaaaataa ttattgccgg
    22141 acgattgggg agaggattcc cattcatagt tttatttagg atgtatgtta tcaagtttac
    22201 acgcccgtcc ggtgtaaggt taatggtttt atacagcata ttgactcctc cttagataaa
    22261 ttgattctcc tgcatttt
//
LOCUS       DAOSVE010000022        22205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_1523_length_22205_cov_71.352957, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000022 DAOSVE010000000
VERSION     DAOSVE010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 22205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22205
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1523_length_22205_cov_71.352957"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PK016_03165"
     CDS             <1..363
                     /locus_tag="PK016_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HXL02105.1"
                     /translation="RMLTMEQIYHIRFEHREKDKSLRRIAQETGFNFRTVRKYVERED
                     FNIKKKAIHTRKGKLDPYKETIDKWLTEDLKARPKQRHTAQRVYNRLKEIYQDDFQVS
                     DRELSLCSKEKERAIFRK"
     gene            complement(743..2476)
                     /gene="glgP"
                     /locus_tag="PK016_03170"
     CDS             complement(743..2476)
                     /gene="glgP"
                     /locus_tag="PK016_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HXL02106.1"
                     /translation="MSIYMFDKLKNEPIIAYLSMEIALSSDIPTYSGGLGVLAGDTVR
                     SAADLSLPFVAVTLISRKGYLNQKISAEGWQIEQPVEWEIEKILTPLPVQVEVEIEKR
                     KVKVGAWVYPWKSARGIIIPVIFLDTNIPQNAPEDRSITDYLYGGDSRYRLKQEIVLG
                     IGGSRILKALDLEVRKYHINEGHAALVTIELLQSFKNWDVEAVKNLCVFTTHTPVEAG
                     HDKFPYDLVKDVLGEPIPLSLLQQLGGIDCLNMTLLALNLSGYINGVAKKHREVSLHM
                     FPGYDIHSITNGVHSFTWTSPTFQTLFDRYIPGWAQEPELLTRADIISNQEIWVAHQK
                     EKTQLFQLVKEKNNIEMDEEVLTLGFARRMTAYKRPHLIFTDIERLKKINRAGKLQIV
                     FAGKAHPYDFEGKKLIQQIIQYSRELSSEIKIAFLENYNLEVARKIVAGVDVWVNTPM
                     KPLEASGTSGMKAAHNGVINFSVLDGWWIEGCLEGITGWSIGPGSEEEENPQETFQKE
                     IDDFYGKLEYVIVPIYYHHREQWIKMMKNSISKIASYFNAHRMMESYITEAYFHQPFL
                     PLSNGLMVKTE"
     gene            complement(2506..2937)
                     /gene="dut"
                     /locus_tag="PK016_03175"
     CDS             complement(2506..2937)
                     /gene="dut"
                     /locus_tag="PK016_03175"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HXL02107.1"
                     /translation="MKEVVIRFKRLSERAFLPCFSFSDDACFDLFSPVDTTIIPQESR
                     IIDLEIASEFPRGYEVVLRPRSGMGINHGIMVHLGTIDCGYRGSWKVRLFNLGKEIYG
                     IKRGDRIIQGALREIPRVKILETNSLSPSERGECGLGSTGR"
     gene            3020..3553
                     /locus_tag="PK016_03180"
     CDS             3020..3553
                     /locus_tag="PK016_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02108.1"
                     /translation="MVIVREETRFSLSLLGVAAIALTVETLIMRIFSFIFGYHFVSLV
                     ISLALLGYGAAGSLYSFLPRRFKEHIPYILAFSLLLLALGIVFLPLDIYKFWVNYYNW
                     FYFAILLFLAFLPFFVHGSYQLYAFELYPSHFPRFYATNSVGSSLGIGISYLILLFFL
                     ALFSLANVTRMKKIPLF"
     gene            3916..4158
                     /locus_tag="PK016_03185"
     CDS             3916..4158
                     /locus_tag="PK016_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02109.1"
                     /translation="MTSFLKLEKLDWITSSTLFASFLKDNFFPYSEPRIISPRKFLAQ
                     GGKYDLIFLEVPIPQATVFPGSFSLQEDLLFTEEGL"
     gene            4282..4929
                     /locus_tag="PK016_03190"
     CDS             4282..4929
                     /locus_tag="PK016_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02110.1"
                     /translation="MDKHLVIIKNLDFALLVIKKSPWRENEIKKIKEKVKEEHFDFTY
                     YPDIKKEEAEIVFQTRKRYYQIVEDILSGKETRSTFDLRPSRDNRPYFSNFFRLEQLR
                     EAWINLGKRWLPFGGSGFWLVVLILVLVITLAFFLIFFPAKTKRKYSLCSKNFESGRN
                     VDWSKFYDDRNFSIYKTGDDSRFAFLHFFSSPSGSPGFFWVGKLAHKAGIEFPRS"
     gene            5350..6156
                     /locus_tag="PK016_03195"
     CDS             5350..6156
                     /locus_tag="PK016_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HXL02111.1"
                     /translation="MRIIAIANQKGGVAKTTTCINLGASLAYHARKVLIIDMDPQAHS
                     TLGLGFEPDEFNKTVLNILEPPRSPYHLELKEVIIPTRTPNLYLAPANIDLAGAEYRL
                     IDKLGREDFLNSSIQRANFSFDYILIDCPPSLGILTVNALKACREVIVTIQPHYFAMR
                     GIEEFMETISLMRENLNHDPEVYVLITIADVRTNLYREVMEEIKKYFGDHLFETVIHR
                     NISLAEATSHGVPVMEYDPSSSGAQHYLNLAKEVIKLEEEKPGKRLLRKN"
     gene            6113..7423
                     /gene="ftsZ"
                     /locus_tag="PK016_03200"
     CDS             6113..7423
                     /gene="ftsZ"
                     /locus_tag="PK016_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HXL02112.1"
                     /translation="MKKKSLERGFSERIDFKRELIRDTTKGIRREESLSSKKEEQKAS
                     SEVVKKNLEKDKTKIEKKAEDRLAIIKVVGIGGGGNNAVNHMAELGLDGVDLIAVNTD
                     IQSLRKSLAQEKLQIGLNLTRGLGTGGDPRLGEEAARQDKEKLSQLVEKADLVFVAAC
                     MGGGTGTGAAPVLANISKESGALTIGVVTKPFNFEGLKRTRQAEEGINRLQKEVDALI
                     VIPNERLFQITKKDTPLQEAFRTADHILYQAVRGITDLITSPQEINLDLADLRTVLSG
                     AGMVLIGIGHGRGREKAKQAAEEAIQSPLLEISVKGAKSIILNITGGQDMTLSEVTEI
                     VNTVTNAAGTETDILWGYRVDDTLSNETMVTVIATRFEVSEEKAGEIEETIDEIDSKI
                     IIEDELDIPAFLRETQKRKEVSEEKRSGDRMFPFFWSGGKNDSE"
     gene            7447..8538
                     /locus_tag="PK016_03205"
     CDS             7447..8538
                     /locus_tag="PK016_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HXL02113.1"
                     /translation="MANLWGKEMTREEILKRVGDISQIGGVKLAELSEGAERGVRIAE
                     VNSGGGLIYTVLLDRGLDIAWTSFKGINISWRSATRNLAPSFFEPEGLGWLRGFHGGL
                     MNTCGLTYAGAAGRDKSTHPFLEEEDFGLHGRASYLPATNLWVDGAWQGDDYLMWVQG
                     KIRESIVFGDKMALYRKIWSRLGGATIHIEDQVVNEGWNESPFMILYHINFGYPLLDE
                     GARLFLPAEKTVPRDKMAEEGKEEWHLFSSPQKGYFEKVYLHYPKTLEDGYSASLLVN
                     NKLKTGVYVKFSKDTLPYFTEWKMMGEGEYVIGMEPGNCFPLGRRREREERRLVTLQP
                     GESKKIYLEIGILEGEEKIEEFKKYLKMG"
     gene            complement(8535..10619)
                     /locus_tag="PK016_03210"
     CDS             complement(8535..10619)
                     /locus_tag="PK016_03210"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HXL02114.1"
                     /translation="MKVREPLFFGLFLIIVGTVAFFNVNFSLANETDLYSLFQQGDYE
                     TIVSLKPGEGEELFLYLVSLLNLNREEEVEKEISSLLPEKDYFWLDHLLYLSSLHFIE
                     KEDLDKAVIWEKRLENYFPSSPLLPYITLRLAQSFEENNLFNSALLYAMYTFTYSLKE
                     EDKLSALKITFQSLAHLGYLKEATFLLRRTYYAFSTLSSQETRQLIQPFISQIFPSDF
                     SLEERLSLADFFFSLGFTEKAKDFLDQIEKYLPEKEEEIFTLHARILVREENWKELEQ
                     LINENLLKRGEKEEILFYWGVLEQRKANYGKATQIYERLLFLYPESEYAFNTFQNLAF
                     SFRVMGKEKGYLETMEKIISRFPGKSAPIWELFWFYYNKNQMLSAQEMLEKLKSFPEE
                     RNKALFWWFKIDSSPEHLEEIVKSGVIDYYYARAWQELEERGFFPSFPFGEEKPFPQV
                     DPTNISYPEHWEKYQFLKKIGMWENAEIEVLFLLFQNSQKKGFYRELSELYTQKGDYQ
                     KSILYSLYLQEGEEIPLNLARRIYPEFFLSSIQKFIGDELDPYLILALIRAESFFNSA
                     AVSSAGAIGLAQIMPSTASWVIEKGWADGGEEDISLLLLNAEKNLEIGVSYFSYLWER
                     FGGKLYPSICSYNAGPGRVDQWLETLPPDPDLFVESIPFSETQNYLKKVIANYFAYSI
                     IYQGRFSPPPYL"
     gene            10664..12391
                     /gene="polX"
                     /locus_tag="PK016_03215"
     CDS             10664..12391
                     /gene="polX"
                     /locus_tag="PK016_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HXL02115.1"
                     /translation="MRNREVAQLLENIATLLEMKGENRFKVVAYEEAAHRIENWPEPI
                     EEVWKEGRLKEIPGIGDSIASKIEEYLSTGKLSYLEELTKEIPPEIIHLTAIPGVGPK
                     IAKLLYDQLGVKSIEDLEKAIAEQKLRHLPRLGVKSEEKIRKGIEMLKRQSGRMLLGQ
                     ALPLVEEIVGALRERSGVTKISPAGSVRRMKETIGDIDILVASSAPEPIMEIFTSLPQ
                     VREVIARGITKSSIVTQEGIQVDLRVVEETSFGAALQYFTGSKAHNIKLREIALKKGF
                     KVNEYGIFRLDNDKRVGGEKEEEIYETLGMEWIPPEMREDQGEIELAQEKKLPILIDI
                     SDLQGDLHVHSNWSDGVTSIEEMAEAALSRGYKYLAICDHTQSLAVASGLNPEEIRER
                     RKEIELWNKKGKGLRLLDGVEANIMSDGSLDFPDSELEKLEVVVAGLHSGLGQGEEKI
                     LHRLEGAMKNKYVQMISHPTGRILNKRDAYQVNREKLFSLARETGTYLEINSQPERLD
                     LSDGDARRARDLGVKLVISTDAHDPSSLDYIRYGVAQARRGWLQKEDVLNTFNLEKLL
                     EFLDRKKKN"
     gene            complement(12388..13122)
                     /gene="nadE"
                     /locus_tag="PK016_03220"
     CDS             complement(12388..13122)
                     /gene="nadE"
                     /locus_tag="PK016_03220"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HXL02116.1"
                     /translation="MRDWKEETQKIITWMRKKVEEAKAQGLILGMSGGIDSSVVAVLA
                     QKALGKKSLGIMMPCYSIPQDLEDALKVAQMFSIPHRIIPLEKPYDAFLEILGEKQKN
                     NRSLALANLKPRLRMCTLYYFANRLNYLVCGASNRSEITVGYFTKYGDGGVDILPIAH
                     LTKSEVRELARFLDIPSFIITKPPSAGLWEGQTDEEEMGITYEKIDEFILHKKVEEPY
                     LSRIKNKIRESEHKRKMPATLEKIAT"
     gene            complement(13135..14613)
                     /gene="xylB"
                     /locus_tag="PK016_03225"
     CDS             complement(13135..14613)
                     /gene="xylB"
                     /locus_tag="PK016_03225"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HXL02117.1"
                     /translation="MEQFLGIDLGTQGVKVVGWDEEGNFLGSASREYSILTPQIGFAE
                     QDPETWWEKTKEAIKELVSSIPQVNIKGIGFSGQMHGTVIIDDNLKPLCPAIIWADQR
                     THQEVKIIRENLKDKLATICGGDVATGFMAPTLLWLKENQKEIFSRIRWAILPKDYLK
                     IKMGLTPSTDVADASSTLLFNIRKRNWSKEILEILELNHSIFPPAYESTQVIGQMKEE
                     IKEELGLKGKVFLIAGAGDQHCAALGNGITEEGELLVTIGTGGQVFTPLYEPLVDDKL
                     RIHTFCHAIPAFWHLLGATLCAGLSLSWFKKSILDSPLQAFDFKELDREAQKVEAGSQ
                     GLIFLPYLIGERTPYMDPRARGAFLNLHLTHGRAHFIRAIMEGVCLGLRNAIEVFKEL
                     GVKPQHIVFSGGGAKSALWRKIMASVLDTPLFTTQVREEAATGACILAMIGIENSSEE
                     DIISRIIRYGNPVFPEKKAAEIYAELYLKFRDAYPALKNLFP"
     gene            14762..16426
                     /gene="argS"
                     /locus_tag="PK016_03230"
     CDS             14762..16426
                     /gene="argS"
                     /locus_tag="PK016_03230"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HXL02118.1"
                     /translation="MSRLITEMLKEEMEKVLAVNFPGIVEEREITVEPTKEKNHGDFA
                     TNLAFILSRKVRKPPRELAINLQSFLSRSLEKFARVEVAGGGFINFFLEDEILLSFIQ
                     EVEKEEEDFGTVDLGKGKKTQVEFVSVNPTGPLHVGHGKCAAFGDSLSRILKKAGFQV
                     EKEYYINDAGRQIDLLGASLEARFRQLLGEKVEVPEDGYQGEYLIPIAEELLQEKGRE
                     ILNLSPSERLEVFRTFAVQKILNSIRHDLDDFRVHFDVWFRESSLYQRKEAEEILSLL
                     KERGYTYNKDGALWLRTTDWGDDKDRVLVRENGLPTYFASDIAYHWNKWERGFQLVID
                     IWGADHHGYIPRMYAAMRALGLPEDFLEIFIVQFVTLLRRGQPERMSTRQGEFVPLRQ
                     LIDEVGVDVARYYFLMRDPATHLEFDIEEAKKQSMDNPVYYVQYAHARIASLFKEAEK
                     RGFVSFPLADTLSRFPSEEEREIVKKIAYFPETVKRSALLRQPYLICNYLQDLCGDFH
                     SFYNLYRIIDEDNVELTYFRLNLCRAVQIVLKNAFQLLGISAPDTM"
     gene            16518..18149
                     /locus_tag="PK016_03235"
     CDS             16518..18149
                     /locus_tag="PK016_03235"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013289447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HXL02119.1"
                     /translation="MDKGKYIFVTGGVVSSLGKGITAASLGRILKSRGFRVTMQKFDP
                     YINVDAGTMNPYQHGEVFVTYDGGETDLDLGHYERFIDEELSRLSNVTTGKIYSSVIE
                     KERRGDYLGATVQVIPHITNQIKEEIFRLREESKADISIVEIGGTVGDIEGLPFLEAI
                     RQIKNDIGKSNCLYIHVTLVPYLEAAGELKSKPTQHSVKELRSIGIQPDIIVCRSSHP
                     ITPEVMAKISLFCDLDKDAIIPLMDVDSIYEVPLVLEEKKVGDLIASRLEMKSTSSDL
                     GEWSQIVQKVKKADKEVNIGLVGKYTESKDAYLSVCEALKHGGIESGLKVSIKSVEAE
                     KLEGNEASCFLSKVDGIIIPGGFGQRGIEGKIRAVRYARENGIPFLGICLGMQAAAIE
                     FARNVLHFYSAHSTEFDPLTPYPVIDLLPSQKDAQKMGGTMRLGNYRCVLENSLSKSL
                     YKREEVWERHRHRYELNNLFVNDLERAGLRMAGYNPDYQVVEIIELKNHPFFIGVQFH
                     PEFKSRPNQAHPLFCGFVEAAYCYQQERRRNRLGI"
     gene            18139..18960
                     /gene="murI"
                     /locus_tag="PK016_03240"
     CDS             18139..18960
                     /gene="murI"
                     /locus_tag="PK016_03240"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HXL02120.1"
                     /translation="MEFKRPLGIIDSGIGGLSVVNAVRALLPAEDIIYIADPLNFPYG
                     EKGKEELLEIVKPIIGYLFQDRKVKLIITACGTLSSLCLPDLAQLINIPIMGIVQPAC
                     QEAAKVTKQGRIGVLATRATVKAGAFCRTLKEINPDIEVIEEAWPEFVAAVEKGKFNS
                     PEWKRQIKNRLISLKERGVDTLIMGCTHFSLISWFFQELGGKELQVVDPAWVCAQETK
                     NKLYSERETGSSSGKLSIFVRGDGFSFRKSLENLPFNLKEEINLFPVDSGVNIGV"
     gene            18953..19849
                     /gene="ispE"
                     /locus_tag="PK016_03245"
     CDS             18953..19849
                     /gene="ispE"
                     /locus_tag="PK016_03245"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HXL02121.1"
                     /translation="MSEKFILPSYGKINWFLKIGEIRPDGYHDIESLMQKIAFGDEIE
                     VALEERDHITCNFPIPTGEDGILGRLLIELRKIAPPLRKVGFNIKIKKNIPPGSGLGG
                     GSSNIASILQLINSVFKLNLDWEEVKSIAVRIGSDVPFFARGAPFAWVRGRGEKVEPL
                     FFVPQRFLVLLFPPFSISTAWAYQCWEERKGEFKKSKKVGMSLKEFLKVKNPELIEEI
                     IWNDFEEVIFQNLPDLNSYRRLLLESGCKKVFMSGSGSTLVGVVENKEEGEKIVRKTS
                     ASNVKVILTSTWGAKEGGENYD"
     gene            19842..20591
                     /locus_tag="PK016_03250"
     CDS             19842..20591
                     /locus_tag="PK016_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HXL02122.1"
                     /translation="MTDAIVLAGGGEGEIEKKFGVTNRSLLVIKDKFMIEYVIEALKS
                     VSSIKRIIVVGPINEIKSRIGGLVEEVVPPGKNPFESAWKGLEVLQTREKVLVSGSDL
                     PLLKGEMVEDFLLRCSKEEADFYYPIIRREVYEKKLNDSQRTFVRLRDGCFTGGDILF
                     LNPQVMEKKKEWISRIIENRKNPVALARLIGAKIIFKYFTKQLTIKDVEERVDKVLGI
                     RALAVITPYPEMGFDVDKVGHVQVAERILKK"
     gene            20676..21944
                     /gene="rho"
                     /locus_tag="PK016_03255"
     CDS             20676..21944
                     /gene="rho"
                     /locus_tag="PK016_03255"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HXL02123.1"
                     /translation="MQFSLADLKRKTNAELFEIAQSLGISGYSRKRKNDLVFEILKKS
                     AESKGYLFSEGVLEITPEGYGFLRTSDDFSPSDSDVYVSPSQIKRFMLSVGDKIAGQV
                     RPPKEGERYYALLRIEAINDEDPDQAKKRPLFENLTPIFPDEQLVLETVSEEISTRII
                     DLFVPIGKGQRGLIVSPPKAGKTVLLEKIANGIMANYPEVVLIVLLVDERPEEVTQME
                     RSVKGQVIASTFDQKPENHLRVAELTLERAKRLVEEGKDVVILLDSITRLARANNLVV
                     PTTGKTLSGGIDSSALHWPKHFFGAARNIEEGGSLTIIATALIDTGSRMDEVIYEEFK
                     GTGNMELHLNRALAESRIFPAIDIHRSGTRKEELLLKKEDLERIWMLRKLLSTVETQE
                     ATQMVIERMRNTRSNKEFLKIIDQVIKASR"
BASE COUNT         6793 a         3933 c         4766 g         6713 t
ORIGIN      
        1 aggatgctaa caatggagca aatatatcat atcagatttg aacatcggga aaaagataaa
       61 tcattgagaa gaatagccca agagaccgga tttaacttcc gaaccgttcg gaagtatgtg
      121 gaaagggagg attttaatat caagaaaaaa gctattcata cccgtaaggg taaactggat
      181 ccctataaag aaaccataga taagtggctt actgaagacc taaaagcccg acctaagcaa
      241 aggcatactg cccaacgggt ctataatagg ttaaaagaga tatatcagga tgacttccaa
      301 gtttctgata gggagttaag cttatgtagc aaagaaaaag aaagagctat cttccggaag
      361 taaagcctat cttcccttag agcataaacc aggagaatct caggtagact ttggggagtg
      421 tcagtttata gaacgaggta ttaaatatga tggtttctat ctcaacctct ctttccctca
      481 cagtaatgga ggatattctc agttattcag agctcaatgt atttggcaat tccattttta
      541 acttccgata caatttgttt ttttaaagtt ttagcagttt aacaggtttt tgtaggatac
      601 tataaaaggt ctcaggcaat agagggaaga gtagaggtct atagaaagca aaaatgctca
      661 agttttgtat tacaaatggc aggataaccc tgcagatgtg gaaccaaggt tttactgatg
      721 agcttataac actacataag ctttattctg ttttgaccat caagccattt gataaaggaa
      781 gaaagggctg atggaaataa gcttcagtta tgtaactctc catcattcgg tgagcattga
      841 agtaagaagc aattttagaa atagaatttt tcatcatctt aatccattgc tctcggtggt
      901 gataataaat agggacgatg acatattcta acttaccata gaaatcatct atctcttttt
      961 ggaaagtctc ctgaggattc tcctcttctt cacttcctgg tccaatagac catccggtta
     1021 ttccctcaag gcacccttct atccaccatc catcgagcac actgaaatta attacaccat
     1081 tgtgagctgc tttcattcca cttgtgcctg aagcctccag cggcttcata ggggtgttta
     1141 cccagacgtc cactccagca actattttcc tggctacttc taagttgtaa ttttctaaga
     1201 aagctatttt tatttctgaa cttaattctc gagaatattg aattatttgc tgaataagtt
     1261 ttttaccctc aaaatcatag ggatgagctt taccagcgaa tacaatctgt aattttcctg
     1321 cgcgatttat tttttttagt ctttctatat cagtaaagat aaggtggggt cttttataag
     1381 cagtcatacg gcgagcaaaa cctaaagtaa ggacttcttc atccatttct atattatttt
     1441 tttctttaac taactgaaag agctgggttt tttctttttg atgagccacc cagatttcct
     1501 gattagatat aatatcagct cgagttaaaa gctctggttc ttgagcccat ccaggaatgt
     1561 aacgatcaaa aagcgtctgg aaggtagggg aggtccaggt aaaactgtgt actccattag
     1621 taatggaatg gatgtcataa cccgggaaca tatgaagaga aacctctcta tgttttttgg
     1681 ctactccgtt aatatatccg ctgagattta aggctaaaag agtcatgttc agacagtcta
     1741 tccctcctaa ctgttgaaga agagaaaggg ggataggttc acctaacaca tcttttacca
     1801 ggtcataagg aaatttatca tgtcctgctt ctactggggt gtgggtagta aaaacacaca
     1861 agtttttaac tgcctctaca tcccaatttt tgaaactttg taacagctct atggttacca
     1921 gggcagcatg tccctcatta atatggtatt tccttacttc taaatccagg gctttcaata
     1981 tcctacttcc tcctatacct agaacaattt cttgtttcag gcgataacga ctatctcccc
     2041 catagagata gtcagttatg gaacggtcct caggagcatt ttgagggata ttggtatcca
     2101 agaaaattac tggtattatt attcctctgg cacttttcca gggataaacc catgctccca
     2161 cttttacttt ccttttttcg atttctactt ctacctgaac aggaagggga gtcagaattt
     2221 tctcgatttc ccattccacg ggttgttcga tttgccatcc ctcagcgctt attttttgat
     2281 ttaggtagcc tttgcgactt atcagagtta cagccacaaa aggaagagaa agatcggctg
     2341 cagaacgaac tgtatcccca gccagaactc ccagcccccc actataggtg ggtatatccg
     2401 aagaaagagc tatttccata gataaataag ctataattgg ctcgttttta agcttgtcaa
     2461 acatatatat actcatagga ttatctgttt cctccctttt gaaatttagc ggccggtaga
     2521 acccaggcca cattcccctc tctcgctagg agataatgaa ttagtctcta aaattttaac
     2581 ccggggaatt tctcgcagag ctccttggat aattctatct cctcttttta taccataaat
     2641 ttctttacct aaattgaaaa gtctgacctt ccagctacct cgatatccac agtcgatagt
     2701 acccagatgt accattatac catggttaat acccattcca cttcgaggcc gcaatactac
     2761 ttcataccct cgagggaatt cggaagcgat ttctaaatct ataattctac tctcttgagg
     2821 aatgatggta gtatccacag ggctgaagag atcgaaacag gcatcgtcag aaaaagaaaa
     2881 acaaggaaga aaagctcttt ccgaaagtct tttaaatctt attactactt ctttcatagt
     2941 tttcctcccg ctaaattaat acaggttata ttttagcaga cctatcaagt ggttttctta
     3001 agagttaaaa tatgtaatag tggttatagt gcgggaggaa acaagatttt ctctttctct
     3061 tctgggagta gctgcgatag ctctaacagt ggaaactctc attatgagaa tatttagctt
     3121 catttttggg taccattttg tttccttagt tatttcttta gctcttttag gttacggggc
     3181 agcgggaagc ctttactctt ttctcccccg gcggtttaaa gagcacattc cttatattct
     3241 cgcattttct cttttactcc ttgccctagg gatagtcttc ttgcctttag atatttataa
     3301 attctgggtc aattactata actggtttta ttttgctatc cttctctttt tagctttttt
     3361 gccttttttc gttcatggtt cgtatcagtt gtatgctttt gaactttatc cctcacattt
     3421 tcctcgcttt tatgctacca attcggtggg ctcttcctta gggataggga taagttactt
     3481 aatccttctc tttttcctgg ctcttttttc tctggcaaat gtaaccagaa tgaaaaaaat
     3541 tcctcttttt tagagataat tataggagtt actttatttt tttctcctct cgaaatttat
     3601 ctttctcctt acgttccttc tcgagcaatt aaagaagtag agaaaaacca gctcgttaaa
     3661 gtttatcata gtccggcaga atcactagaa gtttttttat actccttatt ccagactggc
     3721 atcgggattg agctctaagt tccctgcctt tcctccccct tcctggactt tagtttatga
     3781 taaacaccaa tcctggcttt ttcctgttta ccctgatttt ctcttttatc ccatacctta
     3841 tattatttac ctgtagacat actttccccc agacaaattc tcattgtgga aggcagaagc
     3901 ggtttagagg ggtatttgac ctcttttcta aaattagaaa agttagattg gataacttct
     3961 tctacccttt ttgcttcttt tctgaaggat aactttttcc cctattcaga accgcggatt
     4021 atttctcccc gtaagttttt agctcaagga ggaaaatatg atttaatttt cctggaagtt
     4081 cctatacctc aagcaaccgt tttccccggt agtttctctt tacaggagga ccttttattt
     4141 actgaagaag ggttatgaag cgtcttttct tccctatctg aagaaagagt agcggtgttt
     4201 tctctctttc ttcagaatcc tcctgcagta ctccctaagc tggtaagcct tattaaagaa
     4261 tcctggaggg aagaaaaaaa tatggataag cacctggtga taataaagaa tttagatttt
     4321 gccctattag taattaagaa atcaccatgg agagaaaacg aaataaaaaa gataaaagaa
     4381 aaagttaaag aagagcattt cgattttact tattatccag acataaagaa agaagaagct
     4441 gagatagttt tccaaactcg gaagagatat taccaaatag tagaagatat actttcagga
     4501 aaagaaacaa ggtctacttt tgaccttcgt ccttcccgag acaatcgtcc ttatttttct
     4561 aatttttttc gcctggaaca gctacgggaa gcttggataa atctgggaaa aagatggtta
     4621 ccttttggag ggagtggctt ttggttggtg gtattaattt tagttctggt tataactttg
     4681 gctttctttc taatcttttt cccggcaaag acaaaaagaa aatactccct ctgcagtaaa
     4741 aattttgaaa gcgggaggaa tgttgactgg agcaagtttt atgatgatag aaatttctct
     4801 atttataaaa ctggagatga tagtaggttt gcctttttac actttttctc ttctccttct
     4861 ggttctcctg gttttttctg ggttgggaag ctggcgcaca aggcggggat tgaatttccg
     4921 agaagttaaa aggttatctg gctacaccct tcagttcttt ttgtttattc tggatttctg
     4981 ctccttttag aagagaggct actccattta cctgtctggg aaagcttact ggtcactctt
     5041 ttacctctgg ccggggtggc ttatctttgt ggattaccct ttcctttact ttcttatctg
     5101 tattctactt ttcatccccg cttttttgga caggtttttg cctggaatgg ctttttgtca
     5161 gtcatttcct ccctgattgt tcatttactt tctgtattct ggggattgaa aaattctctg
     5221 ctattagcag tatttatcta cttaattttt tggttattaa tggtatttta ttaccgaaca
     5281 tttgccctga attgtggtat gatatcatag taaaaattat aaggggaata agctttctga
     5341 gataatgcta tgcgaataat tgccattgct aaccaaaaag ggggagtggc gaaaaccacc
     5401 acctgtataa atttgggagc ttctttagct tatcatgcac ggaaagttct catcattgat
     5461 atggatcctc aagctcattc tactctgggc ttgggttttg aacccgatga atttaataaa
     5521 accgttctca atatactgga accacctcgt agtccttatc atctcgaact taaggaagta
     5581 attattccta cccgtactcc caatctctac cttgctccag ctaatatcga cttagctgga
     5641 gcggaatatc gtttaattga taagctgggt agagaagact ttttaaatag tagtattcag
     5701 agagccaatt tctcttttga ttatatcctc attgactgcc ctccctcttt ggggatttta
     5761 actgttaatg ctcttaaggc ctgtcgggag gtaattgtaa ccattcagcc tcattatttt
     5821 gctatgaggg gaattgagga atttatggaa actataagtt tgatgagaga aaatttaaat
     5881 catgacccag aggtctatgt tcttatcact attgctgatg ttcgtaccaa tctttatcga
     5941 gaggtgatgg aagaaataaa aaaatatttt ggagaccatc tttttgaaac tgttattcac
     6001 cgcaatattt ccctggctga ggctaccagc cacggagtgc cggttatgga atatgatcct
     6061 tcttcttctg gagctcagca ttacttaaac ttagccaagg aggtgatcaa gcttgaagaa
     6121 gaaaagcctg gaaagaggct tctccgaaag aattgatttt aagagagaat taattcggga
     6181 tactactaaa ggtataagac gggaagaaag cctatcttcc aagaaagagg aacaaaaagc
     6241 ctcaagcgaa gtagttaaaa agaatttgga gaaagataaa acaaaaatag aaaagaaagc
     6301 agaagataga ttagcaataa taaaagtagt aggaattgga ggaggaggta acaacgcggt
     6361 taatcatatg gctgagctgg gtttagatgg agtagacttg atagcagtta ataccgatat
     6421 tcagtcttta aggaaatccc tggctcaaga aaaattgcaa attggtttaa accttacccg
     6481 gggtttggga acggggggag acccccgctt gggagaagaa gcagccagac aggataagga
     6541 aaaattaagc cagttagtgg aaaaagccga tctggtattt gtagctgctt gcatgggagg
     6601 aggcaccggt acaggagcag ctccggttct ggctaatata tctaaagaat caggagctct
     6661 gactatcgga gtggtgacca aaccatttaa ttttgagggt ttgaagagaa caaggcaggc
     6721 cgaagagggt attaatcgat tacaaaaaga agttgatgct ttgattgtaa ttcccaatga
     6781 gcggcttttc cagatcacta aaaaagacac tcctctacaa gaggctttta gaacagcaga
     6841 ccatattctt tatcaggcag tgcggggtat tactgacctt atcacttctc ctcaggagat
     6901 taatttagat ttggctgact tgagaactgt tctcagtgga gcaggtatgg tcttaatcgg
     6961 aataggccac ggaaggggaa gagaaaaagc caaacaagca gcagaagaag ctattcaaag
     7021 ccctctttta gaaatctcgg tcaaaggtgc taaatctatc atccttaata tcaccggtgg
     7081 ccaggatatg actttaagtg aagttacgga gattgttaat actgttacca atgctgctgg
     7141 aacagaaact gacattttgt ggggttaccg ggtggatgat accctgtcta acgaaacgat
     7201 ggtaacagtt attgctactc gctttgaagt ttcagaagaa aaagcagggg aaatagaaga
     7261 aacaatagat gagatagata gtaaaattat aatagaagat gaattagaca ttcctgcttt
     7321 tttgcgggag acgcaaaaga gaaaagaggt atcggaagaa aagaggtcag gagatagaat
     7381 gtttcctttt ttctggtcgg gcgggaaaaa tgattcagaa taaatgactc taggaggaat
     7441 aactatatgg ctaacttatg gggaaaagaa atgaccaggg aagagatctt aaaaagagtg
     7501 ggcgatatct ctcaaattgg aggggtgaag ctagcggaat taagtgaggg cgcagaaaga
     7561 ggggtaagaa tagccgaggt taattccggt ggagggttaa tttatactgt tcttttagat
     7621 cgaggactgg atatcgcctg gaccagtttt aaaggcataa acattagctg gcgttctgct
     7681 actcgaaatt tagctccctc cttttttgag ccagaaggtc tggggtggct gcgagggttt
     7741 catgggggat taatgaatac ttgtggccta acctatgctg gagctgcagg acgagataag
     7801 tctactcacc cctttttaga agaagaagat tttggtttac acggtagagc ttcttatctt
     7861 ccagctacca acctgtgggt agacggagct tggcaaggag acgattactt gatgtgggta
     7921 cagggaaaaa taagggaatc tatagtgttt ggagataaaa tggctcttta ccgaaaaatc
     7981 tggagccgtt taggaggggc aactatccac attgaagacc aggtagttaa cgaaggctgg
     8041 aatgagagcc cgtttatgat tttatatcat atcaactttg gttatcctct cttggatgag
     8101 ggtgctagat tattcctccc ggcagagaaa actgtacctc gagataagat ggcagaggaa
     8161 ggaaaagaag aatggcatct tttttcctct cctcaaaagg gatattttga aaaagtttat
     8221 ctccattatc ccaaaacctt agaagatggt tatagtgctt ctttattggt gaataataaa
     8281 ctcaaaacgg gggtctatgt taaatttagt aaagatactc tcccttattt tacggagtgg
     8341 aagatgatgg gagaaggaga atatgtgata ggcatggaac caggtaattg ttttcctctg
     8401 ggtcgacgtc gagaaagaga agaaagaagg ctggtaactc ttcaacccgg ggaatctaag
     8461 aaaatttatc tagaaattgg tattttagag ggagaagaaa aaatagagga gtttaaaaaa
     8521 tatttaaaga tgggctaaag gtagggagga ggactaaatc taccctgata aatgatagaa
     8581 taagcaaagt aattggctat aactttcttt aagtaatttt gtgtttctga aaaaggaatg
     8641 gattcaacaa aaaggtcagg gtcaggaggg agagtctcta accattggtc tactctacct
     8701 ggcccggcat tataagagca aatgctggga tagagctttc ccccgaaacg ctcccagaga
     8761 taggaaaaat agctcactcc aatctctaag tttttttcag catttaaaag aagaagggaa
     8821 atatcttctt ctccaccatc tgcccaccct ttttctatta cccatgaagc ggtagagggc
     8881 ataatctgag ctaaacctat ggctccagca gatgaaacag ctgctgaatt aaaaaaactt
     8941 tccgcccgga taagagcaag aatcaaataa gggtctaact catctccaat aaatttctga
     9001 atagaagata gaaaaaattc tgggtaaatc cgccgggcta agttaagagg gatttcttct
     9061 ccttcttgaa gataaagaga ataaagaatg cttttttgat aatctccttt ttgagtatat
     9121 aattcactca attctcgata gaaacctttc ttttgggaat tctgaaagag gagaaataga
     9181 acttctatct cagcattttc ccacatccct attttcttta aaaactggta tttttcccag
     9241 tgctcagggt aagaaatatt agttggatct acctgaggaa agggtttctc ttctccaaag
     9301 ggaaaagaag gaaaaaatcc tctttcttcc agctcttgcc aggctcgggc gtaatagtaa
     9361 tctatgactc cactttttac tatttcttcc agatgttctg gcgaagagtc aattttaaac
     9421 caccagaaaa gtgctttatt tctttcctcg gggaaacttt ttagcttttc taacatctct
     9481 tgagcggaaa gcatctgatt tttgttgtag tagaaccaaa atagttccca gatgggtgcg
     9541 ctctttcctg gaaaacggga gataattttc tccatcgttt ctaaatatcc tttttctttt
     9601 cccataaccc ggaaggaaaa agctaaattc tggaaagtat taaaagcata ttcactctcg
     9661 ggataaagaa ataataatct ctcataaatt tgggttgctt ttccataatt ggctttcctt
     9721 tgctccaaaa ctccccaata gaatagaatc tcttcttttt ctcctctttt taataaattt
     9781 tcgtttatta gctgttctaa ctctttccag ttttcttctc ttaccaaaat acgagcatga
     9841 agagtaaata tctcttcttc cttttcggga aggtacttct ctatctggtc taaaaaatct
     9901 ttagcttttt cggtaaagcc cagagaaaag aagaaatcag ctaaagaaag acgctcttcg
     9961 agagagaaat ctgaaggaaa gatttgagaa ataaaaggtt ggatgagttg tcgagtttct
    10021 tgagagctca gggtagaaaa agcataataa gtgcggcgaa gtaaaaatgt agcttcttta
    10081 agatagccga gatgagctaa agattgaaag gttattttta aagctgataa tttgtcctct
    10141 tcttttaggg aataagtaaa agtgtacata gcatataaaa gagcggaatt aaagaggttg
    10201 ttttcctcaa aagattgagc taaacgtaag gtaatataag gtaaaagagg ggaagaagga
    10261 aagtagttct ctaatctctt ttcccatatg acagctttat ccaggtcctc tttttctata
    10321 aagtgaagag aagagagata aaggagatgg tctagccaaa aataatcttt ttctggcagc
    10381 agggaagaaa tttccttctc tacttcttcc tctcggttaa gattaagaag agatacaaga
    10441 taaagaaaaa gctcttctcc ttctccaggc ttaagagaaa ctatagtttc gtaatctcct
    10501 tgctgaaaaa gagagtacaa gtcagtttca ttagctaaac taaaattaac gttgaaaaaa
    10561 gcaaccgttc ctacaattat taaaaacagc ccaaaaaaaa gcggctctct tactttcatt
    10621 gctttttaat gatagcatat taagagaaag gaggaaagag tgtatgagaa atcgggaggt
    10681 agcacaactc ttagagaata ttgctactct tttagagatg aaaggagaaa atcgttttaa
    10741 agtagtagcc tatgaagaag cggctcatcg catagaaaat tggcctgaac ctattgaaga
    10801 agtgtggaag gaaggaagat taaaagagat ccctgggata ggtgatagca tcgccagcaa
    10861 aattgaagaa tatctctcca ctggtaagtt atcttatctg gaagaattaa ctaaagaaat
    10921 ccctcctgaa attattcatc ttaccgctat accgggggtg gggcctaaaa ttgctaagct
    10981 tctttatgac cagttgggag taaagagcat tgaggactta gaaaaagcta ttgccgagca
    11041 gaagttacgt catcttcctc gtttgggagt aaaaagcgaa gaaaaaatcc gtaaaggcat
    11101 agagatgctt aaaagacaaa gcgggagaat gcttctaggt caagctcttc ccctggtaga
    11161 agaaatagtt ggagctcttc gagagagaag tggggtaacc aaaattagcc cggcaggaag
    11221 tgttcgccga atgaaagaaa ctatcggaga tattgacatt ttagtagctt cttctgctcc
    11281 tgaacctata atggagattt ttacttcttt acctcaagta cgagaggtaa ttgcccgggg
    11341 aattactaaa tctagcattg ttactcagga aggaatacag gttgatttgc gagtggtgga
    11401 agagacctct tttggagcag ctctacaata ttttaccggc tccaaagcac acaacatcaa
    11461 gttaagagaa attgccctta aaaaggggtt taaagtgaat gaatatggaa tattccggct
    11521 ggataatgat aaacgagtgg gaggagaaaa agaagaggaa atttatgaaa ctttaggtat
    11581 ggaatggatt cctccagaaa tgagagaaga ccaaggagaa attgaactgg cgcaggagaa
    11641 gaaattgcct atcctcattg acattagtga ccttcaagga gacctgcatg ttcactctaa
    11701 ctggagcgac ggagtaactt ccatagagga aatggcggaa gcagctctct cccggggcta
    11761 caaatatctg gctatttgtg accacactca atctctggcc gtagcttcgg gtcttaatcc
    11821 cgaggaaatt agagaaagaa gaaaggaaat tgagctatgg aataaaaaag ggaaaggcct
    11881 tcgactttta gatggagtag aagccaatat tatgagtgat ggttctcttg atttccctga
    11941 ttctgaacta gaaaaattag aagtagtggt ggcgggatta cattctggtc taggacaggg
    12001 ggaagaaaaa atcctgcacc gcctggaagg agcaatgaag aacaaatatg ttcagatgat
    12061 cagtcatcct accggacgca ttcttaataa gagagatgct tatcaagtaa atcgagaaaa
    12121 acttttttct ctagctaggg aaaccggaac ctatttagag attaattctc aacctgagcg
    12181 tctggattta agtgatgggg atgctcgacg agctcgagat ttaggggtga aactggttat
    12241 ttctaccgat gcacatgacc cttcttctct ggattatatt cgctatggag tagcccaagc
    12301 tcgccgaggc tggttgcaaa aagaagatgt actgaatacc tttaatttag aaaaactact
    12361 ggagtttctt gaccgtaaga aaaaaaacta agtagcaatt ttttccaatg tagcaggcat
    12421 cttccttttg tgttcacttt cccggatttt gttttttatc cgggaaagat aaggctcctc
    12481 cactttttta tgaaggataa attcatcaat tttttcataa gtaatcccca tttcttcttc
    12541 atcagtttgc ccctcccata gtcctgcaga aggaggcttt gtgatgataa aagaagggat
    12601 atcaagaaag cgagctaatt ccctcacctc gcttttagta agatgagcaa tgggtaaaat
    12661 atctactcct ccatcaccgt atttggtaaa ataacccacg gttatttcac ttcgattaga
    12721 ggctccacaa actaaataat ttaatcggtt agcaaagtag taaagcgtac acatccgcag
    12781 acggggtttt aagttagcca gagctaaaga acggttattt ttttgttttt ctcctaaaat
    12841 ttccaagaaa gcatcataag gtttttctaa agggataatt cgatggggaa tagagaacat
    12901 ttgcgctact tttaaagcat cttctaaatc ctgaggaata ctatagcagg gcatcatgat
    12961 tcccagagat ttttttccca gggctttttg agctaaaacg gctactactg aggaatcaat
    13021 gcctccgctc atccccagta tcaatccttg agctttagct tcttctactt tttttctcat
    13081 ccaggtaatg attttttgtg tttcttcctt ccaatctctc ataattttct cctgttaggg
    13141 aaataaattt tttaaagcag gataggcatc tctaaattta agatatagtt cagcataaat
    13201 ttctgctgct tttttctctg ggaaaacagg gtttccgtag cgaatgattc tagaaataat
    13261 atcttcttct gaagaatttt ctatccctat catagccagg atacaagcac cggtagctgc
    13321 ttcttccctc acctgggtgg taaaaagagg ggtatcaaga accgaggcca tgatttttct
    13381 ccataaagcg cttttagctc ctcctccgga aaacactatg tgttgcggct tcactcctag
    13441 ttctttaaac acctctatag catttctcaa ccccaggcat actccctcca tgatggcacg
    13501 aataaaatgg gctctcccgt gagtaaggtg aaggttaaga aatgcccccc gagctcgggg
    13561 atccatataa ggcgttcttt ctccaatgag ataggggaga aaaatcaaac cctgggaacc
    13621 agcttccact ttttgagctt cccgatccaa ttccttaaaa tcaaaagctt gaagcggaga
    13681 atcaagaatt gattttttaa accagctaag agagagtcca gcacaaagag tagcacctaa
    13741 aagatgccag aatgcaggaa tagcatgaca gaaagtgtga atgcgcaact tatcatcgac
    13801 cagaggttcg tagagagggg taaatacttg tcctccagtt cctatagtta ctaaaagttc
    13861 tccttcttca gttatgccat ttcccagagc tgcacaatgc tggtcacctg ctccagctat
    13921 taaaaacact ttccctttta aacctaattc ttctttaatc tcttctttca tctgaccaat
    13981 tacctgagtg gattcataag caggaggaaa gatgctgtga tttaactcta aaatttctaa
    14041 aatttctttt gaccagtttc tttttcgaat attaaaaaga agagtggaag aagcatcggc
    14101 tacatcagta gaaggggtaa gacccatttt aatcttcaaa taatccttgg gtaaaattgc
    14161 ccaccttata cggctaaaaa tttctttctg gttttctttg agccataata gagttggtgc
    14221 cataaagccg gtagctacat cgcctccaca aatagtagct aatttatctt ttaagttttc
    14281 ccgaatgatt tttacttcct ggtgagtacg ctggtctgcc cagatgatag caggacaaag
    14341 gggtttcagg ttatcatcta ttattactgt tccatgcatt tgaccggaga agccaatgcc
    14401 tttgatgtta acttggggaa tagagcttac cagctctttg attgcctctt tagttttttc
    14461 ccaccaggtt tcaggatcct gttcagcaaa cccgatttga ggagttaaaa tggagtattc
    14521 acggctggcg gaacctaaga aattcccctc ttcgtcccaa cctactactt taactccctg
    14581 agttcctaaa tcaatgccca aaaactgttc cattcttttt tcccctttga tttaacgaaa
    14641 taaaacaact cgtttccttt attatgttta tcaagtataa cataataaaa tacccattga
    14701 accctggttt aaaaaaatat ataatgacca gagttaaatc caagatttaa ggaagaggtc
    14761 tatgagtcgc ctgattactg aaatgttaaa agaggaaatg gaaaaagttc tggcggtaaa
    14821 ttttccggga atagtggaag aaagagaaat aactgttgag cctactaagg agaagaacca
    14881 tggtgatttt gctactaact tagctttcat tctttctcga aaagtaagga agcctccccg
    14941 agaattagct attaatttac aatcgtttct ttcccgaagc ttggaaaaat ttgcccgggt
    15001 ggaagtggca ggaggagggt ttattaactt ctttttagaa gatgaaattt tattatcttt
    15061 tattcaggaa gtggagaaag aagaagaaga ttttgggaca gtagacctgg gtaagggcaa
    15121 gaaaactcag gtagagtttg tgagtgtcaa tcctaccggt cctttgcatg tgggtcacgg
    15181 taaatgtgct gcttttggag atagcttatc tcgcatctta aaaaaagctg gttttcaagt
    15241 ggaaaaggag tattatatta atgatgcagg gaggcagatt gatcttttgg gagcttcctt
    15301 ggaagcgcgt tttcgtcagc ttttaggaga aaaggtagaa gtcccagaag atggttatca
    15361 aggagaatat ctcattccta ttgcagaaga attattacag gaaaaaggaa gggagatttt
    15421 aaacctttct ccttctgagc gtttggaagt gtttcgaacg tttgcagtgc aaaagatttt
    15481 aaatagtatt cgtcatgatt tagatgattt tcgagtgcat tttgacgtat ggtttcgaga
    15541 gagttcttta taccagagga aagaggcgga agaaatatta tcccttctta aggaacgagg
    15601 atatacttat aataaggatg gtgctctctg gctccggact actgattggg gagatgacaa
    15661 agatagagta ttagtgcgag aaaatggatt gcctacatat tttgcttctg atattgctta
    15721 tcactggaat aagtgggaaa gagggttcca gctggttatc gacatctggg gagcagacca
    15781 tcacggttat atcccgcgaa tgtatgcagc aatgcgtgct ctgggtcttc ctgaagattt
    15841 tttggaaatc ttcattgttc agtttgttac tctcctgcga agaggacagc cagaaagaat
    15901 gtctacaagg cagggtgagt ttgtacctct cagacagttg attgacgagg taggagtgga
    15961 tgtagcccgc tattattttt taatgagaga cccagctact catctggagt ttgatataga
    16021 agaagctaaa aaacagtcca tggataaccc ggtatattac gtccagtacg ctcatgctcg
    16081 cattgcttct ctatttaaag aagcagaaaa gaggggattt gtgtcttttc ctctggctga
    16141 tactttatct aggtttccca gcgaagagga aagagaaatt gtcaaaaaaa tagcttattt
    16201 tccggaaaca gtgaaacgca gtgctctctt gcgccagcct tatttgattt gtaattatct
    16261 tcaggatttg tgtggagatt ttcactcttt ttataatctt taccggataa ttgatgaaga
    16321 taatgtagag cttacttatt ttcggctgaa cctttgccga gcggtacaga ttgtactgaa
    16381 aaatgccttt caacttttgg gaatctctgc tcctgacact atgtaaaaat aactaaaatt
    16441 ctttcttaac tgaggggaat cattctctcc ttaaagtgaa aacttagtga taccatactg
    16501 gataaaagga gttaaaaatg gataagggaa agtatatatt tgtaactggt ggagtagtat
    16561 cgtctttagg gaaaggtata accgctgcct cattagggag aattttaaaa agtagaggat
    16621 ttcgggttac catgcagaag tttgaccctt acataaatgt tgatgccggc actatgaatc
    16681 cttatcaaca tggagaagta ttcgtaactt acgatggagg agaaactgac ttagatctgg
    16741 gacattatga gcggttcatc gatgaggagt tatccagatt aagcaatgta actaccggga
    16801 aaatttatag ttcagttatt gaaaaagaaa gaagaggtga ttacctggga gctactgtcc
    16861 aggtaattcc ccatataact aaccagatta aagaagagat tttccgatta agagaggaaa
    16921 gtaaagcaga tatctccatt gtggagattg ggggaacagt aggagatata gaagggctgc
    16981 ctttcttaga agctattcgc cagataaaaa acgatatagg aaaaagcaat tgcctttata
    17041 ttcatgtgac tctcgtgccc tatcttgaag cagccgggga actaaagtct aaacctactc
    17101 agcatagcgt aaaggaatta cggagcattg gtattcagcc agatataata gtttgccgct
    17161 cttctcatcc tatcaccccg gaagtgatgg ccaagatttc ccttttctgt gacctggata
    17221 aagatgccat aatccctctt atggacgttg attctattta tgaggtacct ttagttctgg
    17281 aagaaaaaaa agtaggagat ttgatagcca gtcgtttaga aatgaagtcc acttcttctg
    17341 atctgggaga gtggtctcaa attgttcaaa aggttaaaaa agcagataaa gaagtaaaca
    17401 ttggtttagt aggaaagtat accgaatcaa aagatgctta cttaagtgtt tgtgaggcgc
    17461 ttaaacatgg agggatagag tcaggattaa aagtaagcat taagtcagta gaagcagaaa
    17521 aactagaggg taatgaggca agttgttttc tttctaaagt ggatggtata attatccccg
    17581 gaggttttgg tcagcgagga atagaaggta aaattcgagc tgttcgatat gcccgagaaa
    17641 acgggattcc ttttttagga atatgtctgg gaatgcaggc agcagcgatc gagtttgctc
    17701 gaaatgttct tcatttttat agtgctcatt ctactgaatt cgatccctta actccttatc
    17761 cggtgattga tttattacct tctcaaaagg atgcccaaaa aatgggtggg accatgcgtc
    17821 tgggcaacta tcggtgtgtt ttagagaatt ctctgagtaa atctctttat aagagggaag
    17881 aagtttggga gagacatcgt catcgttatg agcttaataa cctgtttgta aatgacctgg
    17941 aaagagcagg gttgcgaatg gctggttata atcctgatta ccaagtagtg gaaataatag
    18001 agttaaaaaa tcatcccttc ttcatagggg tacaatttca ccctgagttt aaatctcggc
    18061 ccaatcaagc tcatccttta ttctgtggtt ttgtggaagc agcttattgt tatcaacaag
    18121 aaagaaggag aaatagactt ggaatttaaa cgtcctctgg ggataatcga ttcaggtatt
    18181 ggtggtttaa gtgtagtaaa tgcggtcaga gctcttttac cggcagaaga tattatctat
    18241 atagccgatc ctcttaattt tccttatgga gagaaaggta aagaagagtt actggaaata
    18301 gtaaaaccca ttattggtta tctattccaa gatagaaaag taaaattaat tattactgct
    18361 tgtggcactc taagctctct atgtcttcct gatttagctc aattaataaa tattccgatt
    18421 atggggattg ttcagccagc ctgccaggag gcggcaaaag taacaaaaca aggcagaata
    18481 ggagtactgg ctacccgggc tacagtgaag gcgggagctt tttgcaggac tttaaaagag
    18541 attaatccgg atatagaagt aattgaagaa gcctggccag aatttgttgc tgcagtggaa
    18601 aaagggaaat tcaatagccc ggaatggaaa aggcaaatta aaaataggct tatctctttg
    18661 aaagagagag gagtggatac cttaattatg ggttgcactc atttttctct tatcagctgg
    18721 ttttttcaag aactgggggg aaaagaactg caagtggtag accctgcctg ggtctgtgct
    18781 caggaaacaa aaaataaatt atattctgag agagaaacgg gctcatcttc aggtaagcta
    18841 tctatttttg tgcgaggcga tggtttttct tttcggaagt ctttagagaa tttgcccttt
    18901 aatttgaaag aggaaataaa tctttttcct gtcgattcag gtgtgaatat cggtgtctga
    18961 aaagtttatt ttgccttcct atggaaaaat caactggttt ttaaaaattg gagaaatacg
    19021 ccctgatggt tatcatgata ttgaatcgct aatgcaaaag attgcttttg gagatgaaat
    19081 tgaagttgct ttagaggaaa gagaccacat tacctgtaat tttcccattc ccaccgggga
    19141 agatggtatt ctgggcaggt tattgataga attacgtaaa atagctcccc ctcttcgaaa
    19201 agtcggtttt aatataaaaa tcaaaaaaaa cattcctccc ggaagcggac tggggggagg
    19261 aagtagcaat atagcttcta ttttgcaact gattaattca gtttttaagt taaatctgga
    19321 ctgggaagaa gttaaatcta ttgctgtccg tattggttca gatgttcctt tttttgctcg
    19381 gggggcgcct tttgcctggg tgagaggaag aggagaaaaa gtagagcctc ttttttttgt
    19441 tcctcagcgg tttttggttt tgcttttccc tcccttttct atttctactg cctgggctta
    19501 tcaatgctgg gaagagagaa agggagaatt taaaaaaagt aaaaaagttg gaatgagcct
    19561 taaagaattt ctaaaagtaa agaatccaga actgatagaa gaaataattt ggaatgattt
    19621 tgaagaagta atttttcaga atcttccgga tttaaactct tacaggaggt tgcttttaga
    19681 atcagggtgt aagaaagtct ttatgagtgg tagtggttct actctggtag gggtggtgga
    19741 aaacaaagaa gaaggtgaga agatagtgag aaaaacttcc gcttccaatg ttaaagttat
    19801 tttgacttct acctggggag caaaagaagg aggagaaaat tatgactgat gccatagttt
    19861 tagctggtgg aggagaagga gagattgaaa aaaaatttgg agttactaat cggtccctac
    19921 ttgtcataaa ggataagttt atgatagagt atgtaataga agctctcaaa agtgtctcct
    19981 ctattaagag aattatagta gttggaccaa taaatgagat aaaaagccga attggtggtt
    20041 tagtggagga agtagtacca ccagggaaaa atcctttcga aagtgcctgg aaaggtttgg
    20101 aagttttaca aactagagag aaagtcctgg tatccggtag tgacttaccc cttctaaaag
    20161 gtgaaatggt agaagatttt ctgcttagat gtagcaaaga agaagctgat ttttattatc
    20221 ctattatccg tcgagaagtt tatgaaaaaa agttaaacga ttctcagaga acctttgtac
    20281 gtttgcgaga tggatgcttt acaggtggcg atattctatt tctcaatcct caggtgatgg
    20341 aaaagaaaaa agagtggatt tctcgaataa ttgaaaatcg caaaaatcct gttgctcttg
    20401 ctcgattgat aggggcaaaa attattttta aatacttcac caagcagtta actatcaaag
    20461 atgtagaaga gcgagttgac aaggtattgg gcataagggc tttagctgtt attactccct
    20521 atcctgaaat gggatttgat gtagataagg tggggcacgt tcaggtagca gagagaattt
    20581 taaaaaaata ataggaggaa tcaaaagtgg ataacaatga taaccaggta aaagagttag
    20641 tggtggagaa agaaacagat aaaaaaatac ccgcagtgca gttttccttg gctgacctga
    20701 aaaggaaaac taacgctgaa ttatttgaaa ttgcccagag tttaggtata tctggttata
    20761 gtcgtaaaag aaaaaacgat ttggtgttcg aaattcttaa aaaatctgct gaatctaagg
    20821 gttacctttt cagtgaaggg gttttggaaa taacccctga gggctacggg tttttgcgca
    20881 cttctgacga tttcagtcct agtgatagcg acgtttatgt ttctccttca cagattaaac
    20941 gctttatgtt aagtgtagga gataaaatag caggacaggt gcgtcctccc aaggaaggag
    21001 aaagatatta tgcccttttg cggattgaag ctataaatga cgaggaccca gaccaggcta
    21061 aaaaacgccc tctttttgag aatctcaccc ctatttttcc tgatgaacag ttggttttgg
    21121 aaaccgtttc cgaggagatt tctacccgta tcatcgatct ttttgttcct attgggaaag
    21181 gccaaagagg attaatagtt tctcctccta aggcgggaaa aactgtgctt ttggagaaaa
    21241 ttgccaatgg cataatggcc aattatcctg aagtagtatt aattgttctt ttggttgacg
    21301 agcggccaga agaagttact caaatggaac gctcagtcaa aggacaagtt atagcttcca
    21361 cttttgatca gaagccggaa aatcacttgc gagtggccga actcaccctg gagcgagcta
    21421 aacgtttagt agaagaaggc aaagacgtgg tcatcctttt agatagtatt acccgtctgg
    21481 cacgagctaa taacctggtg gttcctacca caggaaaaac tctttctgga ggcattgatt
    21541 cttctgctct tcactggcct aagcattttt ttggagcagc tcgcaacatt gaggaaggtg
    21601 gcagcttaac tattatagcg actgctttga ttgatactgg ttcaagaatg gatgaggtaa
    21661 tttatgaaga gtttaaggga acaggtaata tggagcttca cttaaatcgt gctttagcag
    21721 aaagccgtat attcccggct atagatattc atcgttcggg aactcggaaa gaggagctct
    21781 tgctaaagaa ggaagattta gagagaatct ggatgttaag aaagctttta tctaccgtag
    21841 aaactcagga agctactcag atggttattg agcggatgag aaacactcgc tctaataaag
    21901 aatttctcaa aattatcgac caagttatca aagcttcacg atgataggtg tatagctttt
    21961 atagttgtta actacgaaga ttgctatagc cgtttcttcc tcttaagaag gaaatttgtt
    22021 tttagggtgt cgcaagtatg ctaggcctat tttcctaacg tgatttgctt cagttcttac
    22081 ctttaatttt tgagtttgga gggtttggca tcataaatcc gcaaattcag caacaaaggc
    22141 tatgatacat atgatacaat ctgtttccag acctaggaaa ggcatgaaaa actcataata
    22201 tagta
//
LOCUS       DAOSVE010000023        21778 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_1568_length_21778_cov_72.155089, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000023 DAOSVE010000000
VERSION     DAOSVE010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 21778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21778
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1568_length_21778_cov_72.155089"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..719
                     /locus_tag="PK016_03260"
     CDS             264..719
                     /locus_tag="PK016_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HXL02124.1"
                     /translation="MEKIWEKEFDYFNKRVTPYRRAIIEIFEEEGRHLSAEEVYILLK
                     KKQEKAGIATVYRNLDLLSRMGLLHRVNFGDGKEHYEMTKVSPGQHHHHLVCTRCGKV
                     VDYSELVGEEHEFLNRLEKKLSNKHGFLIQSHQIYFYGLCEDCKSNSGS"
     gene            755..1006
                     /locus_tag="PK016_03265"
     CDS             755..1006
                     /locus_tag="PK016_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA domain-containing protein"
                     /protein_id="HXL02125.1"
                     /translation="MVKLEKNKVSLVEMLPGQEGTVIAIDGGQGVVNRLWVMGIFPGR
                     KITKLSSLPAQGPVVIKVGNQELALGRGMAQRVIVEVTQ"
     gene            1012..2712
                     /locus_tag="PK016_03270"
     CDS             1012..2712
                     /locus_tag="PK016_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HXL02126.1"
                     /translation="MVLAGNPNVGKSVIFTQLSGVYAISSNYPGTTVDYSKSAVRKNG
                     TWFELIDAPGTYALSAEAEAEKIAEKLIEEGDIVINVVDATNLERNLYLTFELLEKRK
                     PMILVLNMWDEAKKRGIEIDRQALEKALGVPVIPVVATTGEGIKDLIRALDRAQIPKL
                     IPQDHEKRWEEIGSLLEKVQRFSYREPQWVDFLYRATISPVGGLLIGIGVLVGSFFLI
                     RLIGEGIINYVFDPLFDRFYLPLLEKLDQLLSFSPFLQSLLIGQKIEGVIDLEQSLGV
                     LSTGVYIPVAVVLPYILAFYLILGLLEDIGYLPRLAVLLDGFLHKVGLHGYAIVPTLL
                     GLGCNVPGILGTRVLESEAERFIVATLISVAVPCAAQQALIVGVLGDFGIGPVILVYS
                     VLFVVWMVTALFLKLVVPGYRPALIIEIPPYRLPYPKAVASKLWMRIREFLKDALPIV
                     LLGVLGINLLYTTNIFSHLAETFSPFLSRWLGLPGEAVFALFLGFLRKDLAMSLLIPL
                     ELSLKQLIISSVALAMFFPCVATFVIILRELGWKRLLQATAIMVSLALLVGGFLNVVL
                     "
     gene            3057..4616
                     /gene="murJ"
                     /locus_tag="PK016_03275"
     CDS             3057..4616
                     /gene="murJ"
                     /locus_tag="PK016_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HXL02127.1"
                     /translation="MKEEHSLVKNTLVVGMGTFLSRISGFLREVLISSSFGATWVTDA
                     FLIAYTIPNLLRRLFAEGALSASVVPVFSCYREEKESSQEFISASFTGFTLIVSLVCI
                     LGMIFSPLLVNIIAVGFRGEVEKISLTTDFTIIMFPFLLLVSWSALLMGFLNTLNIFS
                     WSAVAPILFNLGTIFSLWFFRSFLGPYALAWGVILGGIGQFLFQVPPLYRAGFHLHWD
                     WRFWENEGFRKVIGLMLPVSFSLAASQINTIVDRIIASTCQEGAVSALYFADRLMELP
                     LGVFGVALSVAILPSLSQKFSKGEERGWENVLAQGMRWIIFLMLPVAAFLVIFATPLV
                     KLVYERGAFDFIASSMTARALIFYTLGLPFFSLNLLFTRAFYSRGDTRTPVKVSLISV
                     FSNVGLDLLLVRWMDFGGLALASSLAAFLNSFLLILCLSRARGLLKIGTLLPGKWMGK
                     ILAQVASFTFFCQFLQGKFYPGGIERGLFSFIGIIILVSGFYLLLTFLFQMEEGKAIV
                     YLFRNRRNNAG"
     gene            4616..6418
                     /gene="lepA"
                     /locus_tag="PK016_03280"
     CDS             4616..6418
                     /gene="lepA"
                     /locus_tag="PK016_03280"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HXL02128.1"
                     /translation="MDRNKIRNFGIIAHIDHGKSTLADRILEICGAVPKGKMREQFLD
                     RMELERERGITIKAKAVRVEYFSPPRREKYILNLIDTPGHADFSYEVSRSLAACEGVL
                     LVVDASQGVEAQTLAHAHLAISQGLVILPVINKIDLPSADPERAMREIETILGEDHLP
                     FTLVSAKLGTGVEELMEKIVQFIPPPEERDSPFLRALIFDSVYDSYRGVIPFIRVREG
                     EIKKGMEIMMFSTRCSFPVLEVGVFTPEMKEVDSLGPGEVGYVVANIHHLEDSQVGDT
                     ITSFSYPAPSPIPGYKKVKPLVFCSFYPVSYEDYSKLKEALEKLQLNDAALSFEADFS
                     EALGFGFRCGFLGLLHMEITQERMEREFGVEVVATAPHVVYHILTKKGAVLEVKSPKD
                     FPSPGEIEMMEEPYVEMSITVPAEYLGGVMDLCQSRRGIFKDMVYLSEQLVLLRYELP
                     LAEILLDFYDRLKAVSRGYASLDYEFKGYRPAELVRVDILINQEKVDGLSFISPEENA
                     YYRGRELISRLKELIPRQLFPISLQAAIGGKIIAREDIPALRKDVLQKCYGGDITRKR
                     KLLEKQKEGKKRMKAIGKVRIPQEAFLAVMKSEK"
     gene            6415..7560
                     /gene="hemW"
                     /locus_tag="PK016_03285"
     CDS             6415..7560
                     /gene="hemW"
                     /locus_tag="PK016_03285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HXL02129.1"
                     /translation="MKKLSLYFHFPFCVKRCRYCDFVSYPGRDRSFHLKYKEAVFEEI
                     ALKVVEENLQGRELTTIYLGGGTPSLLLPEDVEEILNYLQCFFTFSSSIEITMEVNPE
                     TVNREKINSFRKAGVNRLSVGVQSFNPRFLRFLGRASSLEQIENVLLQIKKARYPSWN
                     LDLLYSLPFQSFSEWQKEVECALAYHPPHLSIYNLTLSPLVPLYQLVKYHPRIFPSLD
                     EEALYFEWAMEKLEREGYKHYEISNFAQPGAECRHNLNYWREGEYLGVGVSAWSYLEG
                     RRQKNTSSLKNYLKRIEEGKSPLTFQEKLEPYQKLAEDIILHLRTKEGIETSYLLARY
                     QREEVENKLKCLEYLFQEGFIIKEAERFYLSKKGILVANQIFQEVLD"
     gene            7589..8272
                     /locus_tag="PK016_03290"
     CDS             7589..8272
                     /locus_tag="PK016_03290"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HXL02130.1"
                     /translation="MIPIRDELPTRKFPVVTVIIIIANVIFFIYELLLGPQLESFFFQ
                     AGLVPAVLWGKARLINPSIPPFLTIFTSMFLHGNLFHLLSNMLYFWIFGNNVEDFLGH
                     FHFLFFYLVCGLAAGLSHALIFPSSTVPTVGASGAISGVMGGYLILFPFARVLVAVIW
                     GFFFNVVRVPAALLLGIWIFLQLIYGLTSLLSPTLGGTAWFAHLGGFVVGCLWCKIAS
                     NYYERKTYW"
     gene            8322..8756
                     /locus_tag="PK016_03295"
     CDS             8322..8756
                     /locus_tag="PK016_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXL02131.1"
                     /translation="MLVEEIMLPEVITVNDFDLIADVVEFLARRKVSGAPVVDLEQRP
                     VGYFSSPLFFRQLIESAQKEIAITGNFISAIKGKIKEFSSQEVSEFMSSDFDYLEADE
                     GLEEAIDLLLDTDLELIPVIQGGKIVGVVNHNRILQYFVERK"
     gene            8772..9965
                     /locus_tag="PK016_03300"
     CDS             8772..9965
                     /locus_tag="PK016_03300"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HXL02132.1"
                     /translation="MNKKTIRDIPQEELKGKRVLVRVDFNVPLDENRQVTDDTRIVES
                     LPTIKYLKERGAKVILVSHLGRPKGKFAEELRMDPVARKLEELLGEKVYKVDDCIGEK
                     VEEAVSKLKDGEVLLLENIRFYPQEEANDEEFARALSRLADIYVNDAFGTAHRAHAST
                     AGVAKFLPAYAGFLMEKELEALGEKLNNPVRPFLAILGGAKVSTKIGVIRKLLEKVDV
                     LLLGGGMAYTFVKALGYAVGNSILEESMIGEASDVLRIARERGVRLELPEDFVVAPDA
                     KEGVETKVVKWNQIPPDMAGFDIGPEAVEKFGRYIQEARTIFWNGPLGVFEVEPFSRG
                     TVAIAKKIAESGAISVIGGGDTIAAIKKAGVEDKITHISTGGGASLEFLEGRALPGVE
                     ALLDK"
     gene            complement(9977..10426)
                     /locus_tag="PK016_03305"
     CDS             complement(9977..10426)
                     /locus_tag="PK016_03305"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HXL02133.1"
                     /translation="MVLPKFLADCMLGKLARWLRILGYDTKYFRYLDDPQIVALARFE
                     HRTLLTKDQALAKSASDVACLVSSSRVEEQIQEVLKTFRLTYPTPFPRCPYCNELLFD
                     LSQEEAKKEVPLFVSLTFFQFKKCPRCHRIYWPGTHWQRIIQTLSSS"
     gene            10481..12040
                     /locus_tag="PK016_03310"
     CDS             10481..12040
                     /locus_tag="PK016_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HXL02134.1"
                     /translation="MPNTKPPYFLGIDVGTQGLRSGIFDSRGNVVALSFYSYPEHHPR
                     PGWAEQDPADWWKAIQDTVRRCVIQGEVQPEEIAALSVSASSCTVLPVDQFGNPKYRA
                     IMWMDVRAFEQAERINATHNPILHYAGGSESPEWMIPKALWLKENLPDVFANSDYLVE
                     CQNWVVFKLTGRWVTSLNNAICKWNYCPPEGGWPVSLLRELNFEEVLDKWPRDLIPVG
                     GRAGELTKGAASDLGLIPGIPVIQGGIDAYAAMVGLDVVHPQRLAMVIGSSTCHLALS
                     ERPIFSPGIWGPYQGAVLPDMWILEGGQSSTGSIIKWFRDNFLQEEFREKEEVENIFD
                     AMDRIAAQIPPGSDGLVILDNFQGNRSPYQDPLARGAIWGLSLSHTRAHILRAMYEGT
                     AYGTFHILETLARDGFQVEEIYASGGGARSKLWLQIHADVADIPIYLTTVEEASTLGT
                     AIYAAVGVGFYGSIPEATLKMVQISGQIYPRRQNRDIYRFYYEQYVKSYWQLRDLMHR
                     VSTRLGAIPGV"
     gene            12180..13499
                     /gene="lysA"
                     /locus_tag="PK016_03315"
     CDS             12180..13499
                     /gene="lysA"
                     /locus_tag="PK016_03315"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HXL02135.1"
                     /translation="MLGNKSINESNHLEIAGCDVVELAQTMGTALYVYDEALLRSNMQ
                     EYEKSFRDNYPHFSLAYAGKAFLCSALCSMMEKEGWWLDVVSGGEYYLASTANFPVER
                     IIFHGNNKTQEERCLVLRGGVGRWVVDNEVELNFLLEEAPLYASYKVPLFFRLIPGVD
                     PHTHQYITTGKIDSKFGFPLGEVENAILKAIQSEDLEVWGLHCHIGSQIDQIEPFLKA
                     VEVMLQFMVDFRKKHGFVFRELDLGGGLGVPYVDEEKGRFPSFASYVKAITEKVKEKC
                     DEFSYPLPHLYIEPGRSVVNTAGSTIYRVGSVKEIPGVKKYVAVDGGMTDNPRPILYG
                     AKYQAQIGNRVVGKKEKVSVVGKCCESGDVLIEEIELPSPYPGDILVVEATGAYNYSM
                     ASNYNLIPRPGVVFLREGRPELVVRPESWHDLVRRDIIPLENREGER"
     gene            13496..14302
                     /gene="dapB"
                     /locus_tag="PK016_03320"
     CDS             13496..14302
                     /gene="dapB"
                     /locus_tag="PK016_03320"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HXL02136.1"
                     /translation="MMKEIGVFLVGAAGKMGREIVRAIQKEEGIVLRGAADKQEVGKD
                     AGELSGVGPLGITIEENLAEAINKVKPDVICDFTQAAALRENLPLFLDGKIPLVIGTT
                     GLSSEEIETLRRTCNDRGIGCVIAPNFALGAVLMMYFSRIAARYLERAEIIEYHHSQK
                     KDAPSGTAIKTAQGIGEIKSTSFSDKSEEILSGARGALLGSVNIHSVRLPGFIAHQEV
                     IWGGEGEILTIRHDSLSRSSFMPGVMRSIKKVYEEPRFYYGLEEVIGLTY"
     gene            14346..15200
                     /gene="dapF"
                     /locus_tag="PK016_03325"
     CDS             14346..15200
                     /gene="dapF"
                     /locus_tag="PK016_03325"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HXL02137.1"
                     /translation="MRFVKMHGLGNDFVIFPEEETEGISSWSSFSLKACDRHRGVGGD
                     GVILVLSSSVADLRMRIFNSDGSEAEMCGNGVRCLAKFAFERGMVNKKEFTVETKAGV
                     IILRITEEEKGKVKKVRVNLGRPRFQPREIPVALEEREVKNYPLEVGDRIFLVSALSL
                     GNPHVVIFEIPPHWEEYGREIENHPLFPEKTNVEFVEVKNNKEAVVKVWERGAGATLA
                     CGTGAAASLIAGVREGKLDRKANIYLPGGPLEVEWQEEVYIEGPAEEVFEGEINEEVE
                     KEWKLLSG"
     gene            15179..16342
                     /locus_tag="PK016_03330"
     CDS             15179..16342
                     /locus_tag="PK016_03330"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HXL02138.1"
                     /translation="MEVAQRIKNLPPYLFAEIERKVNQARAEGKEIIDLGIGDPDIPT
                     PSFIVERLCEEARRPENHRYPSYQGLAAFREAVALWYKKRFGVNLDAEREVVSLIGSK
                     EGIAHLPLCLVDPGDLVLASDPGYPVYKIGTMLAGGIPVELPLLAQNDFLPDLHSFPE
                     SIWEKVKLVFLNYPNNPTASIAPLAFFKEIVKLARRFDFVIAHDLAYSEISYDGYQAP
                     SILEVEGAREVAIEFHSLSKTFNMTGWRIGFAVGNERIIEALGRVKTNIDSGIFNAIQ
                     WAGVEALSRVDEVLEDIIPIYTERRNLVVETFSSIGFDIIPPRATFYLWIPVPPEFNS
                     LEFTNYLLDKSQVVVTPGTGFGKYGEGFVRISLTTPTPVLEKAMVRIKETWDR"
     gene            16349..17575
                     /gene="dapG"
                     /locus_tag="PK016_03335"
     CDS             16349..17575
                     /gene="dapG"
                     /locus_tag="PK016_03335"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HXL02139.1"
                     /translation="MKIVVQKFGGTSVNTRELREKAALKVKETQEEGFSPIVVVSAMG
                     REGAPYATDTLISLAREEFSNLDPRDLDLLLSCGEIISAVVMSQTLRRMGMKSVAFSG
                     AQAGIITDDRFGDASILRVEPDRIKKYLDKGCVVVVAGFQGVTEEGEVTTLGRGGSDT
                     TAVVLGIYLQADYVDIFTDVNGVFTADPHIVPEARILQGLTYTEAAEIIQQGAKVIHS
                     KAMNLAREYRVPLRVRSLSDEGRGTLICDISTLTKEGLRILSQEEPVYSIAYRRGLAQ
                     VRVKLADSGNGELKIFQSLAQQGISIDLVSLFPESVAFVVDENKGSKVEEILNKEGFE
                     VSLTLPCAKISLVGSGMADRPGVVAQLVEALKEANIEILQTGDSHITISCLVKAQDME
                     RAIQILHHKFQLENVC"
     gene            17587..18480
                     /gene="dapA"
                     /locus_tag="PK016_03340"
     CDS             17587..18480
                     /gene="dapA"
                     /locus_tag="PK016_03340"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HXL02140.1"
                     /translation="MRGNFGNLLTAVVTPFDQDWTINYFEFRELLRALVEDGSDGVVV
                     SGTTGESPTLSREEKLRLFEVALEEIGDQAKVIAGTCNYNTKESILLSKEAEELGVHG
                     ILAVAPYYNKPPQEGLYQHFRAIASAVNVPVMIYNIPSRTGVNIEPETIARLAEIPNI
                     VALKEAAGSTDQISRIKALVPSDFYLYSGDDFMTLPLLALGGVGVVSVASHLVGKEIK
                     KMIQLFNEGKTKEALEIHLELYPLFKALFISTNPIPVKAALNLVGWRVGGVRPPLSPL
                     DKEKEEKLKSVLEKIHSVNSL"
     gene            complement(18772..19251)
                     /locus_tag="PK016_03345"
                     /pseudo
     CDS             complement(18772..19251)
                     /locus_tag="PK016_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(19322..20380)
                     /locus_tag="PK016_03350"
     CDS             complement(19322..20380)
                     /locus_tag="PK016_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02141.1"
                     /translation="MKKIFLLLLFAFMFMGNSVSTQELLQYSQKEEYLLVFPDGQVFG
                     QELWPVNFQRGTNQFLWQRTEGEELENVSWKVQGGMLKNITHELNSLTSYLSIEADKD
                     GQGEIILLYPREDIESQFIYQINWEKGNTYPQVLLWVTIKELGSYSSPGVKLKILGRE
                     FTLDLEPQRERRLLFVSTAPEEAEKVIKYHSKEGAAKLFWRLKLPYHLLLPAKVECFE
                     KEGQAVTFLGEGTFYPSGAEYLELPLGEAQDIILKETIVYQEKVNQVRDSTGKEVLYD
                     TKEEKLYHLQNRGEEVKKVIVYDRVFPSLEIISSSLPAELEEADLLSFEIILSPQEEK
                     EIRISVQGKKLTKGWIWN"
     gene            complement(20377..21552)
                     /locus_tag="PK016_03355"
     CDS             complement(20377..21552)
                     /locus_tag="PK016_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02142.1"
                     /translation="MNKKFLSKLTAFVLCFFVLSTITPADAFVSRPLTPEESRIIVYQ
                     GVNKAWIEELFSLNFSSGENKLIFSQEDLAGKRLFLAPLSEEVKVEKIENIPGGQEIS
                     FTARSQGKHQILLAFFEEGWDWREFYDGIIQKEKNELVLHPSILITNQSKRGGKEVFL
                     SWFLGTPLWEEEIPVFSTKAFDERIIEEATSLEVSSAPIMERVSEYQLFTFPERVEIL
                     PLNILQFFLPPQVFIFEEIIRFQEGEIYQILTFENPGEPLPPGIINLFEEDRLLIKKF
                     FPGARTGEKVELSVSSPPLISIKRKNTRYQRKETRFSDEKEVVGFISQREITFEIENQ
                     RDEEIYVELIEPVPAPPNWILTAGWEWKDNQVSKKIFLPPREKRKVILSYQFEEAIP"
BASE COUNT         6026 a         3755 c         5134 g         6863 t
ORIGIN      
        1 ttgtaatgga ttttgcggtt aactatataa tgaagcagaa cttaaataga agcatgaaag
       61 atggcattta atgtggtctt tattggcttg tcctctagaa tataggaaat atcccttttg
      121 ttatatcttt gtaaccctga ggtgtaaaaa taactacaca aacagcctct tccttattgc
      181 tggttgattc tgacagtggt ataatgagaa acaagaatga tattgataat cattatcaaa
      241 ttattgtcag ggggagcaat gttatggaga aaatatggga gaaggagttt gattatttta
      301 acaaaagagt taccccctat cggagagcca tcatagaaat ttttgaagaa gaggggagac
      361 acctttcggc ggaagaagta tatatactct taaagaaaaa gcaggaaaaa gcaggaatag
      421 ctacggttta tcgtaatcta gatttactgt cccgaatggg acttctccac cgggtaaatt
      481 ttggtgatgg taaagaacat tatgaaatga ccaaagtgtc tcccggccag catcatcacc
      541 atttagtttg tactcggtgc gggaaagtag tggattatag tgagttagta ggagaagaac
      601 atgaattttt gaatcgctta gagaaaaaac taagtaacaa acatggtttt ttaatccagt
      661 ctcatcaaat atatttttac ggactctgtg aggattgcaa atcaaattct ggatcttgaa
      721 gtgctgagct tgtaatacct aggggaaagg tagagtggta aagttggaga aaaataaggt
      781 gtctttggta gagatgttgc cgggacaaga aggaacagta atagcgattg atggtggaca
      841 aggggtggtg aatagattat gggtcatggg aatttttccg ggaagaaaga taacaaaatt
      901 gagttctctg cccgctcaag gtccggtagt gatcaaagtg gggaatcaag agctggctct
      961 aggaaggggg atggctcaaa gggtgatagt agaagttacc caataaaaaa agtggttttg
     1021 gctggcaacc ccaatgtggg taaaagtgtt atttttactc aattaagtgg agtttatgcc
     1081 atttcttcca attatcccgg tacgactgtt gattattcta aaagtgcggt tcggaaaaac
     1141 gggacgtggt ttgagttaat tgatgctccg ggtacttatg ctctctctgc tgaagccgag
     1201 gcagaaaaaa tagccgaaaa gttgatcgaa gaaggagata tagtgataaa tgtggtagat
     1261 gctactaatc tggagcgcaa tctttacctc actttcgagc ttctggaaaa gagaaagccc
     1321 atgattttag ttttaaatat gtgggatgag gctaaaaaac gagggattga gatagaccgc
     1381 caggctttag agaaagctct aggagtgcct gttattccag tagtagctac cactggagag
     1441 ggaatcaaag atttgattcg tgccttagac cgagctcaaa ttcccaaatt gattcctcaa
     1501 gaccatgaaa agcgctggga agaaatcgga agtttattgg agaaagtcca gcggttctct
     1561 tatcgagaac cacagtgggt cgattttctt taccgggcta ctatatctcc tgtgggagga
     1621 ctattaattg gaataggagt tttggtagga tctttttttc tgattcgcct aattggtgag
     1681 ggaataatta attatgtttt tgaccctctt tttgaccgtt tttatcttcc tttattagaa
     1741 aagttagacc agctactttc cttttctcct tttcttcaaa gtcttttgat tggtcagaaa
     1801 atcgaaggag ttattgatct ggaacaatct ctgggagtgt tatctactgg agtttacatt
     1861 cctgtagcag tagttctacc ttatatttta gctttttatc taattttagg tctcttagaa
     1921 gatataggtt atcttcctcg gcttgcagtt ctgcttgatg gctttctcca caaagttggt
     1981 ttgcatgggt atgctattgt acctaccttg ctgggtttag ggtgtaatgt cccggggatt
     2041 ttaggcaccc gggttttaga aagcgaagca gagcggttta ttgtagctac tcttatatcg
     2101 gtagcagtcc cctgtgctgc ccaacaggct ctcattgtgg gggtattagg tgacttcggg
     2161 ataggtccgg tgattttggt gtattccgtt ctttttgtgg tctggatggt taccgctctt
     2221 tttctcaaac tcgtagttcc tgggtatcgt ccggcattaa ttatagaaat tcctccttat
     2281 cgcttacctt atcctaaagc agtagcttcc aagttgtgga tgagaattcg agagtttttg
     2341 aaagatgctc ttcccatcgt tttattagga gttctgggga ttaatcttct ttatactact
     2401 aatatttttt cccacttggc tgaaactttc agtccctttc tttcccgatg gttaggatta
     2461 cccggggagg ctgttttcgc tttgtttcta ggctttttgc gtaaagattt ggctatgagt
     2521 ctgcttattc ctcttgagct ttcattaaaa caactgatca tatccagtgt ggcgctggct
     2581 atgtttttcc cctgtgtagc tacttttgtg ataatattac gggagttagg ctggaagagg
     2641 cttcttcaag ctactgcaat tatggtttct ttggcactgc tggtgggagg attcctgaat
     2701 gtagttttat aatttccaga gtcttttctt ttccaacgat gagagcttga agatggagca
     2761 attatccatc cagaaaaggg agaaattgag gtatttcttg ttgtagaagc tttcccctat
     2821 tcctctcgac aaaaattcaa taatgggtta ggctatattg gattggcttt ttgatacaat
     2881 actttgctca aagaaataag cgatgtccac ttttgtttat gttgtctact atagaggaga
     2941 aggttaaaat taagtatttg tcaaagaggg agaggtcagt atatagcgaa gaaaaatatt
     3001 tcctgtcccg ctctcttttc tggccaatat tctttccttc tttaccagga taaaacatga
     3061 aagaagaaca ttctttagta aaaaatactt tagtagtggg aatgggtact ttcctgtccc
     3121 gaatttcggg ttttttaagg gaagttttaa tttcctcttc ctttggggct acctgggtta
     3181 ctgacgcctt tttaattgct tacaccattc ccaatctttt acgtcgactt tttgctgaag
     3241 gagcgttgag tgcttcagta gtcccggttt tttcttgcta tagagaggaa aaagaatctt
     3301 ctcaggaatt tatttccgct tcttttaccg gttttacttt aattgtttcc ctggtctgta
     3361 ttctgggaat gattttttcc cctcttctcg tgaatattat agcggtaggt tttcggggtg
     3421 aggtagaaaa gatatcttta actactgact ttaccattat tatgtttcct tttctccttc
     3481 tggtttcctg gtctgccttg ctcatgggtt ttcttaatac tcttaatatt ttcagttggt
     3541 cggcagtggc accgatttta tttaatctgg gaactatttt ctctctctgg ttttttagat
     3601 cttttttagg cccttatgct ttagcctggg gagtaatttt aggaggaatt ggtcaatttc
     3661 tgtttcaagt tccccctctt tatcgagcgg gtttccatct tcactgggac tggaggttct
     3721 gggagaatga aggtttccga aaagtcatcg gtcttatgct tccggttagt ttttctctgg
     3781 cggcgtcaca aatcaatact atagtggata gaattattgc ttctacctgt caagaaggag
     3841 cagtgtctgc cctttatttc gctgatagat tgatggaatt acccttagga gtctttgggg
     3901 tggcactttc cgtcgccatt ttaccttctc tttctcaaaa attttctaaa ggagaagaaa
     3961 ggggatggga aaatgtttta gctcagggta tgcgatggat tatatttttg atgttacccg
     4021 tagctgcttt tctggtcatt tttgctactc cgcttgtgaa actggtttat gaaagaggag
     4081 catttgattt tatagcttct tctatgactg ctcgggcatt aattttttat actctcgggt
     4141 tacctttttt ctccttgaat cttctcttca cccgtgcttt ttattctcga ggagatacca
     4201 gaactccagt taaagttagt ttaatttcgg tattttctaa tgtagggtta gaccttctcc
     4261 tggttcgatg gatggatttt ggcggtctgg ctttagctag ctctttagct gcttttctaa
     4321 attctttttt acttatttta tgtttaagcc gcgcccgggg attgcttaaa atcgggactt
     4381 tgctgcctgg taaatggatg gggaaaattt tagctcaagt ggcctcgttt actttctttt
     4441 gccaatttct ccaggggaag ttttatcccg gaggaataga aaggggttta ttttctttta
     4501 ttggtattat aattttagta agcgggtttt atcttttact tacttttctt ttccagatgg
     4561 aagaaggtaa ggcaattgtt tacttatttc gtaacaggag gaataacgct ggataatgga
     4621 tagaaataaa atcaggaact ttggtatcat tgctcacatc gaccatggta aatcgaccct
     4681 ggctgataga attctggaaa tttgcggggc agttcccaag gggaaaatga gagagcaatt
     4741 tctagaccgc atggagctgg agcgggaaag aggaataacc atcaaagcaa aggcagtaag
     4801 agtggagtat ttttcccctc caaggaggga aaaatacatt ttgaatttaa tcgatactcc
     4861 tgggcatgct gattttagct atgaagtttc gcggagttta gcagcttgtg aaggagtttt
     4921 actggtggtg gatgcttctc agggggtaga agctcaaact ttagctcatg ctcatcttgc
     4981 tatttctcag ggtcttgtta ttcttccagt aataaataaa attgaccttc cctctgctga
     5041 tcctgaaaga gcgatgagag aaatagaaac tattctggga gaggaccatc ttccttttac
     5101 tctggttagt gccaaactgg gaactggggt agaagagtta atggaaaaaa ttgttcaatt
     5161 tattcctcca cccgaagaga gggattctcc ttttctccgg gcgttaattt ttgattcagt
     5221 ttatgattct tatcggggtg ttattccttt catccgggta agagagggag aaattaaaaa
     5281 aggaatggaa atcatgatgt tttccactcg ctgtagtttt cccgttttag aggtgggagt
     5341 ttttactccg gagatgaaag aagtagatag cttaggtccc ggagaagtag gctatgtagt
     5401 tgctaatatt catcatttag aggatagtca ggtgggagat accattactt ctttctccta
     5461 ccctgctcct tctcctattc cggggtacaa aaaagtaaaa cccctggtgt tctgcagttt
     5521 ttatccggtg agctatgagg attattctaa attaaaagag gctttagaga aattgcagct
     5581 taatgatgct gctttgagtt ttgaagccga tttttcggag gctctgggtt ttggcttccg
     5641 ttgtggattt ctgggccttc tccacatgga aataacccag gaaagaatgg agcgggaatt
     5701 cggagtagaa gtggtggcta ctgctcctca tgtggtgtac catattttga ccaaaaaagg
     5761 ggcagtgtta gaagtgaagt ctcccaaaga tttcccttct ccaggagaaa tagaaatgat
     5821 ggaagagcct tatgtggaga tgagcataac tgtcccagcg gagtatttag gaggagtgat
     5881 ggacctctgc cagtcccggc gagggatttt taaagatatg gtttatttga gcgaacaact
     5941 ggttcttttg aggtatgaac tgcctcttgc tgaaatttta ctcgattttt atgaccgctt
     6001 aaaagcagtg agcagaggtt atgccagctt agactatgaa tttaaaggat atcgtccggc
     6061 agaattggtg agagtggata ttttgattaa tcaagaaaaa gtggatggcc tttcttttat
     6121 ttctccggag gagaatgctt attaccgggg tagggagtta atttccaggt tgaaggagct
     6181 cattccccga caactttttc ctatttcttt gcaagcagcc ataggaggaa aaataattgc
     6241 ccgggaagat ataccggctc tgcgcaaaga tgtactgcag aagtgctatg gtggagatat
     6301 aaccagaaag aggaaactcc tggaaaaaca aaaagaaggc aaaaagagga tgaaagccat
     6361 tggcaaagtt agaattcctc aggaagcttt tttagcggtg atgaaaagcg agaaatgaaa
     6421 aaattatctc tttattttca tttccctttt tgtgttaaaa gatgtcgcta ttgcgacttt
     6481 gtctcctacc cgggaagaga ccggtccttt caccttaagt acaaagaagc agtttttgaa
     6541 gaaatagctt taaaagtggt agaggaaaat ctccagggga gggaattgac caccatttat
     6601 ctggggggag ggactccttc tttgctactt cctgaagatg tagaggaaat actgaactac
     6661 ctccaatgtt tctttacttt ctcatcttct atagagataa ccatggaagt gaacccggaa
     6721 acagtgaaca gagagaaaat aaattctttt cggaaagcag gagtaaatag actcagcgta
     6781 ggagtacaat cttttaatcc ccgttttttg cgttttttag ggcgggcttc ttccttggaa
     6841 caaattgaga atgttttact tcaaattaag aaagctcgct atccctcctg gaatttagac
     6901 cttctttata gcttgccttt tcagagtttc tcggagtggc aaaaagaggt ggagtgtgct
     6961 cttgcctatc atccgcctca tctttccatc tataatctta ctctttctcc tctagttcct
     7021 ctttatcaac ttgttaaata tcacccccgg atttttcctt ctcttgacga agaagctctt
     7081 tattttgaat gggcgatgga aaaattagag agggaaggat acaagcatta tgagatttct
     7141 aactttgctc aaccgggagc agagtgccgc cataatctca attattggag agaaggagaa
     7201 tatctgggag tgggggtttc agcgtggtct tatctagaag ggagaagaca aaaaaatacc
     7261 agtagcttaa aaaactacct caagcggata gaagagggaa aaagtcctct tacttttcaa
     7321 gaaaaactgg agccctacca aaaattggcc gaagatatta tacttcactt aaggactaaa
     7381 gaggggatag aaacctcata tcttttagcc cgataccaaa gggaggaagt agaaaataaa
     7441 ttaaagtgtc tggagtattt atttcaagaa ggttttataa ttaaagaggc agaaaggttt
     7501 tatctttcta aaaaaggaat tttagtagct aaccagatat ttcaagaagt tcttgattaa
     7561 ggagggtagg ttgtgctatt tttagaacat gataccgatt agagacgagt tgccaaccag
     7621 gaaatttccc gtagtaactg taattattat tatagctaat gtgatatttt ttatttatga
     7681 attgcttttg gggcctcagc tggaatcttt tttctttcag gctgggttgg ttccggcggt
     7741 tttatggggt aaggctcgcc ttataaaccc ttctatcccc ccttttctca ccatttttac
     7801 ttctatgttt cttcatggta atcttttcca ccttctatct aacatgctct atttctggat
     7861 ttttggtaat aatgtggaag atttcctggg tcattttcat tttttattct tttatctggt
     7921 ttgtggtctt gcggcaggtc tctctcatgc gctgattttt ccttcttcta ccgttcccac
     7981 agtgggagca agtggtgcta tcagtggagt gatgggtggg tatttaatac ttttcccttt
     8041 tgctcgagta ctggttgcag taatctgggg atttttcttc aatgtagttc gagttccggc
     8101 ggctttactt ctggggatct ggatttttct ccagttgatt tatggcttaa cctctcttct
     8161 ttctcctact ttaggaggaa cagcatggtt tgctcacctg gggggttttg ttgtgggctg
     8221 cttatggtgt aaaatagctt ctaattacta tgaacgcaag acctactggt agtgatttat
     8281 ggtaggaaag attaaataaa ttgataggga ggaatagtta agtgctggta gaagaaatta
     8341 tgttacccga ggttatcacg gtaaacgact ttgatttgat tgctgatgtt gtagaatttc
     8401 tggcccggag aaaagttagt ggagcacctg tcgtagattt agaacaaaga cccgtgggtt
     8461 atttttcttc tccccttttt ttccgacagc ttatagaatc ggctcaaaaa gaaatagcta
     8521 ttactggcaa ctttatatct gctattaaag gaaaaatcaa agaattttcc tctcaagaag
     8581 tttctgaatt tatgagtagt gattttgatt atctggaggc agatgagggg ttagaagaag
     8641 ctatagattt attgcttgat actgacttag agctcatacc tgtgatacag ggggggaaaa
     8701 ttgtaggagt ggtaaatcat aatagaattt tgcaatattt tgtagaaaga aaataaggga
     8761 ggtgcagaaa gatgaacaag aaaaccattc gagatatccc tcaagaagaa cttaagggaa
     8821 aaagagtact ggtgagagtg gattttaatg ttcctctgga tgagaaccgg caggtcaccg
     8881 atgatacaag gattgtagaa agtttgccca ccataaagta tcttaaagaa agaggagcta
     8941 aagttattct ggtttctcac ctcggccgtc ctaaggggaa atttgctgaa gagttgagaa
     9001 tggaccctgt agcccgaaag ttagaagagc tcttaggaga gaaagtttat aaagtagatg
     9061 attgtatagg agaaaaagta gaagaagcag tttctaaatt aaaagatgga gaagtacttc
     9121 ttctcgagaa tattcgcttt tacccccagg aagaagccaa cgatgaggaa tttgctcggg
     9181 ctctttctcg cttagctgat atttatgtga atgatgcttt tgggactgct caccgggctc
     9241 atgcttctac tgccggggta gctaaatttt tgcccgctta tgccgggttt ttgatggaaa
     9301 aagagctgga agctctggga gagaagctca ataatccagt acgacccttt ttggctattt
     9361 taggaggagc caaagtgtca actaaaattg gagtgattag gaaactcctg gaaaaggtgg
     9421 acgttcttct tctaggaggt gggatggctt atacttttgt taaagcttta ggctatgcag
     9481 tgggtaactc tattttagaa gaatccatga taggggaagc ttctgatgtt ctgcgaatag
     9541 ctcgagaaag aggagtgcgt ctggaacttc cggaagattt tgtagtggct ccggacgcta
     9601 aagaaggagt agaaactaaa gtagtaaaat ggaatcaaat ccctcctgat atggcagggt
     9661 ttgatattgg tccggaagcg gtagagaagt ttggtcgcta catccaggaa gctcgaacta
     9721 ttttctggaa tggacctctc ggggtttttg aagtagagcc tttttctcga gggacagtgg
     9781 ccatagccaa aaagattgct gaaagtggcg ctataagtgt aataggtggt ggagatacca
     9841 tagctgctat taaaaaggca ggagtagagg ataagattac tcatatttct accggtggtg
     9901 gtgcttcttt ggaatttttg gaaggacgag ctttgcctgg ggtagaagcg ttgctagata
     9961 agtagaatta taaaggtcag gaactcgaga gggtctgaat tattctctgc cagtgagttc
    10021 ctggccaata tattcggtgg catcggggac attttttgaa ttgaaaaaaa gttagagaaa
    10081 cgaaaagagg gacttctttt ttagcttctt cttgagagag gtcgaaaagt aattcgttac
    10141 aataggggca gcggggaaag ggagtaggat aagtaaggcg aaaagtttta aggacttctt
    10201 gaatttgttc ttctacccga gaagaggaaa ctaggcaagc aacgtcagaa gcactttttg
    10261 ctaaggcctg gtctttagta aggagagttc ggtgttcaaa acgagctaat gccactattt
    10321 gggggtcatc cagatatcga aagtacttgg tatcatatcc caaaattcgc agccacctgg
    10381 ctaacttacc taacatacaa tcagctaaaa atttggggag aaccatgttt ttattttaac
    10441 ataataaagt taattttctt gttatggagg tctgaagatt atgcctaata ctaaaccccc
    10501 ttatttttta ggtattgatg tggggactca gggattacga agtgggattt ttgattcccg
    10561 tggtaatgtg gtagcccttt ccttttattc ttatcctgaa caccatcccc gtccgggatg
    10621 ggcagagcaa gaccctgctg attggtggaa agcgattcaa gatactgttc gccgttgcgt
    10681 tatacaggga gaagtgcaac cagaagaaat tgccgctttg agtgttagtg ctagctcttg
    10741 tactgttctt cctgtggacc agtttggtaa tcccaaatac cgggctatca tgtggatgga
    10801 tgtccgggct tttgaacagg cagaaagaat taatgctact cataacccta ttcttcacta
    10861 tgccggagga agtgaatcac cggagtggat gatacctaaa gctctgtggc ttaaggagaa
    10921 tttacctgat gtttttgcta actctgacta cctggtggaa tgccagaact gggtggtttt
    10981 taaacttacc ggtcgctggg tgacttctct caataatgct atttgcaaat ggaattactg
    11041 tccccccgag ggaggatggc cagtttctct cttgcgagaa cttaattttg aagaagtact
    11101 ggataagtgg cctcgggatt taatcccggt agggggaaga gcaggggagt taactaaagg
    11161 ggcagcttct gacctgggac ttattcctgg tattcctgtt attcagggag gaatagatgc
    11221 ctatgctgct atggtagggt tagatgtggt tcaccctcag agattagcta tggtaattgg
    11281 ctcttctacc tgtcatttgg ctctttctga acgtcctatt tttagccccg gtatctgggg
    11341 accttatcag ggagcagtgt tacctgatat gtggattttg gagggaggac agagttcaac
    11401 cggttccatc atcaagtggt ttagagacaa tttcctgcag gaggaattca gagaaaaaga
    11461 agaagtggag aatatttttg atgctatgga caggatagcg gctcaaattc ctcctggttc
    11521 tgatggtctt gtgatattgg ataatttcca gggtaaccgc agcccttacc aggatccttt
    11581 agctcgaggc gccatctggg ggctttcttt gagtcacacc cgggctcata ttttgcgagc
    11641 catgtatgaa ggcacggctt acgggacatt ccatatttta gagactctgg ctcgggatgg
    11701 ctttcaggta gaagagatat atgcaagtgg tggaggagca aggagtaaac tgtggttgca
    11761 aattcatgct gatgtggctg atattcccat ttacctgacc actgtagagg aggccagtac
    11821 cttggggacg gcaatttatg ctgcagtggg agtaggtttt tatggaagca ttcctgaagc
    11881 tacgctcaaa atggtgcaga tatcaggtca aatatatcct cgtcgacaga atagagatat
    11941 ttatcgtttt tattatgagc aatatgttaa aagttattgg caacttcgag acctgatgca
    12001 tcgggtctct accaggctgg gagctatccc gggagtttaa ttattaaaat atcaaaaatt
    12061 taacttgttt ttataatgtt gatagttaaa aatttatctt cttgagaatc aacaccgtag
    12121 taaaagattg tgcctttgaa gcagttgaat tggagaaatt aaaaagtggg aggaaggaaa
    12181 tgctggggaa taaaagtata aacgagagta atcatttaga gattgccgga tgcgatgtag
    12241 tagaattagc tcaaactatg ggaactgctc tttacgtgta tgatgaagct ttactccgct
    12301 ctaatatgca ggaatatgag aaaagcttta gagataacta tcctcatttt tctctggctt
    12361 atgcaggtaa agcttttttg tgctctgctt tatgctctat gatggagaaa gaaggctggt
    12421 ggttagatgt ggtgtcagga ggggaatatt atcttgcctc cacagctaat tttccagtag
    12481 agcgaatcat ctttcacggt aataataaaa ctcaagaaga gcggtgctta gtccttcgag
    12541 gaggagtggg acggtgggta gtagataatg aagtcgagct aaattttctc cttgaagaag
    12601 ctccgttgta tgcctcctat aaagtccccc ttttttttcg tcttattcct ggagttgacc
    12661 ctcatactca ccagtatatc accactggta aaattgatag taagtttggt ttccccttag
    12721 gggaagtaga aaacgccata ttaaaagcta ttcaatcgga agatttagaa gtgtggggcc
    12781 tccactgtca tattggctca caaattgacc agatagagcc ttttttgaaa gcggtagaag
    12841 ttatgcttca atttatggtt gattttagaa aaaaacatgg ttttgtgttt cgagaattag
    12901 atttaggagg agggttgggg gttccttatg tagatgaaga aaagggccgc tttccttctt
    12961 ttgcttctta tgtaaaagcg atcactgaaa aggtaaagga aaaatgtgat gagttttctt
    13021 accctttacc ccatctttat atagaaccgg ggcgttcggt ggtaaatact gctggtagca
    13081 ctatttaccg ggtagggagt gtaaaagaaa ttccgggggt gaagaaatat gtagctgtag
    13141 acggagggat gactgataac ccccgtccta ttttatacgg tgctaagtat caagctcaga
    13201 ttggtaatcg agtggtaggg aaaaaagaaa aagtttcggt ggtaggtaaa tgttgcgaat
    13261 cgggggacgt tttaatagag gaaattgaac ttccctctcc ttatcctggg gatattttag
    13321 tagtggaggc tactggagct tataattatt ccatggcttc caattataat cttatccctc
    13381 gcccgggagt ggttttcctc cgggagggga ggccggaatt ggtggtgcgt ccggaaagct
    13441 ggcatgattt agtaagaaga gatattattc ctttagagaa ccgagaaggt gagagatgat
    13501 gaaggaaatt ggggtttttt tagtaggagc agcaggtaaa atggggaggg aaatagtaag
    13561 agctattcag aaagaagagg gcatagtttt acggggagcg gccgataagc aggaagtggg
    13621 caaagatgca ggagagctat cgggtgtggg acctctggga attacaatag aggaaaattt
    13681 agctgaggcg ataaataaag taaaacctga tgttatctgt gactttactc aggctgcagc
    13741 tctgcgggaa aatcttcctc tttttctgga tggtaaaatc cctttagtga ttggaacgac
    13801 gggtttaagt agcgaagaaa tagaaactct gcgccgtaca tgcaatgata gggggatagg
    13861 ttgtgtaatt gctcctaatt ttgctctggg agcggtattg atgatgtatt tttctcgcat
    13921 tgctgctcga tatttagaac gagcagaaat aatcgaatat catcactctc aaaaaaaaga
    13981 tgctccttca gggacagcaa taaagactgc tcaggggata ggagagatta aaagtacctc
    14041 tttttctgat aaaagcgaag aaatattatc cggagcgcga ggagcacttt tgggttcggt
    14101 gaatatccac agtgtaagat tgcccggctt tatagctcat caggaagtta tctggggagg
    14161 agaaggagag attctcacca taagacacga ttctctgagc cggtcatctt ttatgccggg
    14221 agtgatgcgc tccattaaaa aagtttatga ggaaccccga ttttattatg gactggaaga
    14281 agttataggt ctcacttatt gattgataga tgtgataaaa tttacctcaa attgaggaga
    14341 gaaaaatgcg ttttgtaaaa atgcacggtt tgggtaacga ttttgttatt ttccctgaag
    14401 aggagactga aggaatctct tcctggtctt ctttttcttt aaaggcttgt gaccgtcatc
    14461 gaggagtggg aggagacgga gtgattttgg ttttatcttc ttcggtggct gacctcagga
    14521 tgcggatttt taactctgat ggtagtgaag cagaaatgtg tggtaatgga gttcgctgtc
    14581 tggctaagtt tgcttttgag cggggaatgg tgaataaaaa agaatttacg gtggaaacta
    14641 aagcgggtgt tattattctc cggattacag aagaagaaaa agggaaagtg aaaaaagtga
    14701 gagtgaatct ggggaggcct cgctttcagc cccgggaaat ccctgtagct ttagaggaga
    14761 gagaagtaaa aaattatcct ttagaggtgg gagatagaat tttccttgtt tcagctctct
    14821 ctttaggtaa tcctcatgta gtgatttttg aaattcctcc ccattgggaa gaatacggga
    14881 gagaaataga aaaccatcct ctgtttcctg aaaaaaccaa tgtggagttt gtagaggtaa
    14941 aaaataataa agaagcagtg gttaaagtct gggaaagagg agcaggagct actctggcct
    15001 gcggaactgg agcagcagca agtttaatag ccggagtaag agagggaaaa ttagatagga
    15061 aagctaatat ctacttaccc ggaggaccac tggaggtaga atggcaagaa gaagtctata
    15121 ttgaaggacc agcagaagaa gtgtttgaag gagaaatcaa tgaggaggta gaaaaagaat
    15181 ggaagttgct cagcggataa agaaccttcc tccttacctt tttgccgaaa ttgagagaaa
    15241 ggtaaatcaa gctcgggcag aaggtaaaga aattatcgat ttagggattg gagatcctga
    15301 cattcccact ccctctttca ttgtagagcg gttgtgtgaa gaggctcgac gccccgaaaa
    15361 tcatcgctac ccttcctatc agggtctggc tgcttttcga gaagcagtag ctttgtggta
    15421 taaaaaaagg tttggagtta atctggatgc agagcgggaa gtggtctctc ttattggctc
    15481 taaagaaggt attgctcatc ttcctttgtg tctggtagac cctggagacc tggttttagc
    15541 tagcgacccg ggttatccag tgtataagat tggtacaatg cttgcggggg gtataccggt
    15601 tgagcttcct cttttagctc aaaatgattt tcttcccgat ttacatagtt ttcctgaaag
    15661 catatgggaa aaagtgaagt tagtgttctt gaattaccct aataatccta cggcgagtat
    15721 tgcccctctt gcttttttta aagaaatagt aaaattagcc cggcgttttg actttgtaat
    15781 agctcacgat ttagcttatt cggagataag ctatgatggt tatcaggctc cgtccattct
    15841 ggaagtagag ggagctcggg aagtagcgat agaatttcat tctctttcta aaacttttaa
    15901 tatgacggga tggagaatag gttttgcagt gggaaatgag agaataatag aagctctggg
    15961 gagagtaaaa actaatattg attcaggaat tttcaacgcc attcaatggg caggggtaga
    16021 agctctgagt cgcgttgatg aggtattaga agatattatt cccatctata ccgagcggcg
    16081 taatttagta gtagaaacct tttcttctat aggttttgat atcattcctc ctcgggctac
    16141 cttttatttg tggattccag ttcctccgga atttaattcc ctggaattta ctaattatct
    16201 cttagataaa tctcaggtgg tggttactcc tggaactggt tttggtaaat atggagaggg
    16261 ctttgttcga atttctctta ccactcccac tcctgtttta gagaaagcta tggtaaggat
    16321 aaaagaaact tgggataggt gaatgaccat gaagatagtg gtgcaaaaat ttgggggaac
    16381 ttcagttaat acccgggagc tcagggaaaa agcagctcta aaagtgaaag aaactcaaga
    16441 agaagggttt tcccctatag tggtagtatc agctatgggt agagaaggag cgccttatgc
    16501 taccgatact ttaatttctt tagctcgaga agagttttct aaccttgacc ctcgagattt
    16561 agaccttctt ctttcctgtg gagagattat ctcggcagta gttatgtctc aaaccttgcg
    16621 tcggatggga atgaaaagtg tagctttctc tggagctcaa gccggtatca tcactgatga
    16681 ccgttttggt gatgcttcca tattaagagt agagccggat agaattaaaa aatatctgga
    16741 taaaggatgt gtagtagtag tagccggttt tcaaggagta actgaggaag gagaagttac
    16801 cactttggga agagggggta gtgacactac ggcagtggtt ttgggaattt atctgcaagc
    16861 tgactatgta gatattttta ccgatgtgaa cggagttttt actgctgacc ctcatattgt
    16921 tcctgaggct cgaattcttc aagggctaac ttacactgaa gctgcagaga taatccaaca
    16981 gggagcgaaa gttattcata gtaaagctat gaatttagcc cgggagtatc gagtgcctct
    17041 ccgggttaga agtttaagtg atgaaggtag agggactttg atttgtgata tatctactct
    17101 cactaaagag gggttgagaa ttttgtctca ggaagagccg gtttatagca tcgcttatcg
    17161 tcggggcttg gctcaggtgc gggtgaaatt agcagattca ggaaacggag agctgaagat
    17221 ttttcaatct ctagctcaac agggtatcag cattgacctg gttagtcttt ttcctgaatc
    17281 tgtggctttt gtagtggatg aaaataaagg aagtaaagta gaagaaatat taaacaaaga
    17341 agggtttgaa gtaagcttaa ctcttccttg tgctaaaatt tctctcgtgg gttcgggtat
    17401 ggctgaccgc ccgggagtgg tagctcagtt ggtggaagct ctcaaagaag ctaatataga
    17461 gattcttcaa actggagatt ctcatattac catttcttgt ttggttaaag ctcaggatat
    17521 ggaaagagcg attcaaattt tgcatcataa atttcaactg gagaatgttt gttaggggga
    17581 gaggaaatga ggggaaattt tggtaatctt ttaaccgcag tggtcactcc ctttgaccag
    17641 gattggacaa taaattactt tgaatttcga gaattattgc gagctttggt ggaagatggc
    17701 tcagatggag tggtagtttc tggaacgact ggagaatctc ctactttatc ccgggaggaa
    17761 aaacttcgtc tttttgaagt agctctggag gaaataggag atcaggcgaa agttatagct
    17821 ggaacatgca attataacac taaagaatcc attcttcttt ctaaagaggc tgaagagcta
    17881 ggagtacatg gcattttggc tgttgctcct tattataata aacctcctca agaaggactt
    17941 taccagcatt ttcgggctat tgcttcggca gtaaatgtcc cggttatgat ttataatatc
    18001 ccttctcgca caggagtgaa tattgagcca gaaactatag ctcgtctagc ggagatcccg
    18061 aatattgttg ctctaaaaga agcagcgggg agcactgatc agatttctcg gattaaagct
    18121 ctggttcctt ccgattttta cctttatagt ggagatgatt ttatgaccct tcctcttctg
    18181 gctttagggg gagtgggagt ggtaagtgtc gcttctcatc tggtgggtaa agagataaaa
    18241 aagatgattc agttatttaa tgagggaaaa actaaagaag ctctggaaat acacctggaa
    18301 ctttatcctc ttttcaaagc actttttatc tctaccaatc ctattccggt aaaagctgct
    18361 ttaaacttag taggatggag agtgggagga gttcgacctc ctttatctcc tctggataaa
    18421 gaaaaagaag aaaaattgaa atcagttctg gaaaaaatcc attctgtcaa tagcttatga
    18481 agttttacct gaaagaggat gacattctgt gataataaaa tcaccctctt tcaggtggca
    18541 ccttttctcc ggtttcaatt cataaataat atagtcatgt tgcacgttta tcccgaatta
    18601 agagctatta tagagtagca ctttttggct tcgcctcact tcaatatgga aaaaagcagg
    18661 ttccgtcttt ttcctgactt tgacacttca cttagctaaa acccacatca aacctatatt
    18721 ctttaaatta gaatctgcta ttttgtcact acactcatta cttccggtag tttatagaga
    18781 gagttcttct ttaggagtga tatttttagg gggtttaatt ttgaggagtt ttaagatttt
    18841 attactcagt tctgttcctt tgattttgat taagaactgt tgattttcca tttctacctc
    18901 agcaaagttt agagagttca aggcttcccg ggtttcttct ggaaaagctt tctcttcgct
    18961 ttcctttaac ttgaactcca gcgtcctctc taaaaggaaa gccagaaagc agataacgaa
    19021 atgtcctttg attctatttt ctgtccaggg aaagataggt ctgacttcta aagtactttt
    19081 cataattcgg aaggattctt ctatcttcca taaagtatgg taagcttcta aagcatcttg
    19141 aggtttgata tcttgctcac tggtctctat accatagtaa ccgtcaaact tctcatctcg
    19201 atgtagagct tcttcatcca gataccaggt ttctttactg gtattcttga gatatttctt
    19261 gccccctctt ttattactgg cagtaactgt tactacaaaa aactctcctt agaaacaaag
    19321 gctaattcca tatccaacct ttggttagct ttttcccttg tacagaaata cgaatttctt
    19381 tttcctcctg aggggaaagt attatttcaa aagagagaag gtctgcttct tctaactcgg
    19441 ccgggagcga agaggaaatt atttccaagc tagggaacac ccggtcatat actataactt
    19501 ttttgacttc ttccccgcgg ttttgcaaat gatacagttt ttcttcttta gtatcataaa
    19561 gtacttcttt tcctgtagag tcccgcacct gattgacttt ttcctggtaa acgatggttt
    19621 cttttaagat gatatcttgc gcttctccca aggggagttc tagatactct gctcctgaag
    19681 ggtaaaaggt tccctctcct aggaaagtga ccgcttgtcc ttctttttca aaacattcta
    19741 ctttggctgg aagtaaaaga tgataaggta attttagcct ccagaataac ttggctgcgc
    19801 cttctttact atggtatttg attacttttt ctgcttcctc tggcgcagta gagacgaaaa
    19861 ggaggcgcct ttctctttgg ggttcaaggt ctagagtaaa ctctcttcct aatattttta
    19921 acttgactcc tggagaagaa taggagccta actcctttat tgttacccag agaaggactt
    19981 gaggataggt gtttcccttt tcccagttta tttgataaat aaactgggat tctatatctt
    20041 cccgagggta gagaagaata atttctcctt gtccatcttt atctgcttca atggaaagat
    20101 aggaagttaa actattgagc tcatgggtga tgttttttaa catccctccc tggactttcc
    20161 aggatacatt ttctaattct tctccctctg ttcgttgcca taagaactga ttggtgcccc
    20221 tttgaaaatt tactggccat aattcttggc caaatacctg accatcaggg aaaaccaaca
    20281 ggtactcttc cttttgactg tattgaagaa gttcttgagt agatacagaa ttccccataa
    20341 acatgaaggc aaaaagtaga agtaaaaaaa tctttttcat gggatagcct cctcaaattg
    20401 ataagataaa attacttttc tcttttctcg gggaggaaga aatatttttt tcgagacttg
    20461 gttgtctttc cactcccatc ctgcagtgag tatccagtta ggaggagcag ggaccggttc
    20521 tattagttct acgtaaattt cttcgtctct ttggttttct atctcaaaag taatttctct
    20581 ctgggatata aaacctacca cttctttttc gtcgctaaag cgagtttctt ttcgctgata
    20641 cctggtattt tttcgcttta ttgaaattag aggaggagaa gaaacagaaa gttccacttt
    20701 ttctcctgta cgggcaccgg ggaaaaactt tttgataagg agtcggtctt cctcgaagag
    20761 attgataatt ccagggggaa ggggttcccc ggggttttcg aaggtaagaa tctggtaaat
    20821 ttccccttcc tggaaacgta taatctcttc aaaaataaaa acctgaggag gaagaaaaaa
    20881 ttgcaaaata tttaaaggta aaatttccac cctctctgga aaagtaaaaa gctggtattc
    20941 agaaactctc tccattatag gagcagaaga aacttccaaa gacgttgctt cttctattat
    21001 tctttcatcg aaggctttag tggaaaaaac agggatttct tcttcccaga gaggagtgcc
    21061 aagaaaccag cttaaaaaga cctcttttcc tcctctttta ctctggttag tgatgagaat
    21121 agaagggtga agtactaatt cgtttttttc tttttggatg atgccatcgt agaactctct
    21181 ccagtcccac ccttcttcaa agaaagccag aagtatttgg tgttttccct gggaacgggc
    21241 agtaaaagaa atctcttggc ctccagggat gttttctatt ttttctactt taacttcttc
    21301 agatagagga gcgagaaata atctttttcc tgctaagtct tcctgagaga agatgagttt
    21361 attttctcca gaagaaaaat taagagaaaa cagttcttct atccaggctt tgttcactcc
    21421 ttgatatacg atgatacgag attcctcggg ggttaaaggc cgagaaacaa acgcatcagc
    21481 aggggtaatg gttgacagaa cgaaaaagca aaggacgaag gcagtcaact ttgaaagaaa
    21541 ttttttattc actttttccc tccttttatc agaatcgttt taattatagc atttttgaca
    21601 tgtacttctt agcttgctat attttccttc ctcagcaatt cacagtgaga tgtagaataa
    21661 ctaaaaaatt tatctattac ctcttgccaa atatgcttta acctattata atatcctttg
    21721 ctttttatcg taccttgaca agtaaataag gtggaaacaa gagatattcc tgctaatc
//
LOCUS       DAOSVE010000024        20084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_1739_length_20084_cov_74.496380, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000024 DAOSVE010000000
VERSION     DAOSVE010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 20084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20084
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1739_length_20084_cov_74.496380"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(540..983)
                     /locus_tag="PK016_03360"
                     /pseudo
     CDS             complement(540..983)
                     /locus_tag="PK016_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062226.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1006..1416
                     /locus_tag="PK016_03365"
                     /pseudo
     CDS             1006..1416
                     /locus_tag="PK016_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013401794.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(1836..2636)
                     /gene="truA"
                     /locus_tag="PK016_03370"
     CDS             complement(1836..2636)
                     /gene="truA"
                     /locus_tag="PK016_03370"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HXL02143.1"
                     /translation="MVLEYDGSRYQGWQRQSRGKTIQGIIEGVGERILQEKITLYASG
                     RTDAGVHATGQIASFTTSTHLSIEAIKKAFNALLPPDIRVVDILDAPLSFHPRKSAVR
                     KIYVYVFSPSLSPVFWQPFVYSFSPRDFNLDLVKECLSLLKGTYDFTSFSSSKGVGTS
                     VRTIYSIQIEEKHRGLLRLWVEGNGFLYNMVRIIAGELWKVGMGKKKPEEIKFMLEHP
                     SRSFNRLVLPSRGLFLMQVCYPPNFNPYQNLQLVDEGWIVPLWREKKV"
     gene            complement(2657..3457)
                     /locus_tag="PK016_03375"
     CDS             complement(2657..3457)
                     /locus_tag="PK016_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009430967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HXL02144.1"
                     /translation="MWGRSLVIGQYIPLDSPIHRLDPRVKIVFVGISIIDLFILQDWF
                     SFLLWGIALIFLVSLSHLSFAYLLRSLRPLLFLLLLTLVLHFFFTPGEYIWEYGFLHI
                     SREGVSKGLLIVTRLCLLILVTSLLTLTTSPVELTDGMEYLLSPLKRWGFPAHELAMM
                     MTIALRFIPTLLEEADRILKAQMARGMNLDSGGIIQRAKNLIPLLVPLFVNSFKRADE
                     LAIAMESRCYRGGKGRTRLRVLELTSSDYYFLALSFSFVFLLSLHSFI"
     gene            complement(3447..4319)
                     /locus_tag="PK016_03380"
     CDS             complement(3447..4319)
                     /locus_tag="PK016_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HXL02145.1"
                     /translation="MFIKLEKVSFTYLPGTPLAITALEDIDLEIERGESIGIVGRTGC
                     GKSTLIQHFNALLIPQSGRVLVDGVDTGKKGVPLQEIRRKIGLVFQYPEDQFFEETVF
                     KEVAFAPHNLGVEGKELEYKVRWALGAVGLNFEAIKDKSPFELSGGQMRRVAIASILS
                     MEPEALVLDEPTAGLDPEGKRIILNQLEKLQKERGLTLIIVSHSMEDLARVAKRIIIM
                     DSGKIKFDDDIRQVFSQVEWIRSSGIFPPVTAQVAWLLRERGFDVTPGVLTPQELAEE
                     IQLKILERKKANVG"
     gene            complement(4310..5140)
                     /locus_tag="PK016_03385"
     CDS             complement(4310..5140)
                     /locus_tag="PK016_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005955923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HXL02146.1"
                     /translation="MIEFKKVNFWYGEEDEPEVGNEEVLREVDLEIPQGKFMVLLGRN
                     GSGKSTLAKHTNGLLLPKSGEVIVDGMNTGDEESIWEIRRRVGLVFQNPDNQIIATTV
                     EEDVAFGPENLGLPREEILARVEEALNLVGMAEYRNKEPHLLSGGQKQRVAIAGVLAM
                     HPEYLILDEATSMLDPEGREELMKVIINLRERIAVFHITHNVEEATEADEVLIMDEGR
                     IVARGKPREIFSQVEKIEEWGLELPQVVETALLIKKQFPSLFEVLPLSPGELVQELCL
                     "
     gene            complement(5171..5524)
                     /gene="rplQ"
                     /locus_tag="PK016_03390"
     CDS             complement(5171..5524)
                     /gene="rplQ"
                     /locus_tag="PK016_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HXL02147.1"
                     /translation="MRHRRRTEKLGRTTAHKKSMMSNLLVSLIKSGKIQTTEAKGKAL
                     KRYFDRVVYLAIKGDNASLREVVSCLRDKEAFKVLFREIAPRFRERRGGYCRLVKMGY
                     RQGDAAPVVLVEIVE"
     gene            complement(5502..6518)
                     /locus_tag="PK016_03395"
     CDS             complement(5502..6518)
                     /locus_tag="PK016_03395"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HXL02148.1"
                     /translation="MLDIRNITRCDLLDWEEDHTTKVQYGKFSIEPLEVGWGITVGNA
                     IRRVLLSSIEGAAVTGVQIEGVIHEFSSLPGVREDITDIILNLKGLVLRSYSDEETLY
                     LEVEGTPGELREVTARDIQPNSNVEIINLDHHLAEIDEDARLSMKISVKRGRGYLEVE
                     GGEIKEDYDFIPVDALFTPVRKVNFQVLDTRVGQFTNYDKLIIEIWTNGAITPQQALS
                     QSLDLLTGEFSRFGELLKEKIAENVSTEEAEEDEEMAEPETSIEELELSVRAYNCLKR
                     AHIDTVQELVNVLQNRPEDLKKIKNLGQKTYEEIVKKVQKWSSEAESREEKGVNKSEA
                     PAEN"
     gene            complement(6535..7155)
                     /gene="rpsD"
                     /locus_tag="PK016_03400"
     CDS             complement(6535..7155)
                     /gene="rpsD"
                     /locus_tag="PK016_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HXL02149.1"
                     /translation="MSRYTDAKCRLCRRHGSKLFLKGPRCYTDKCAMEKRPFPPGVHK
                     GARRRLSSYGLQLKEKQRVRFMYGITENQFKNYFEKANRLPGVTGENLLSLLERRLDN
                     VVFRAGWADSRSDARQMVLHGHFLVNGRKVDIPSYLVDEGDVVEISPRGKENQKIKEL
                     MEEEKTPAPAWLGVGEDGKATILRAPRREDIDYAVEEQLIVEFYSK"
     gene            complement(7171..7557)
                     /gene="rpsK"
                     /locus_tag="PK016_03405"
     CDS             complement(7171..7557)
                     /gene="rpsK"
                     /locus_tag="PK016_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HXL02150.1"
                     /translation="MAKAGRKKKREKRTVREGIAHIKSTFNNTIVSITDKQGNVLSWS
                     SAGASGFKGTKKGTPFAAQLAAEQAAKKAMDQGLREVEVLVKGPGSGRETAIRSLQAA
                     GLIISSIKDVTPIPHNGCRPPKQRRV"
     gene            complement(7593..7961)
                     /gene="rpsM"
                     /locus_tag="PK016_03410"
     CDS             complement(7593..7961)
                     /gene="rpsM"
                     /locus_tag="PK016_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HXL02151.1"
                     /translation="MARIAGVDLPNHKRIDIALTYIYGVGRKLAAKILEETKVDPGIR
                     VKDLSDDELARIQKVVEKYPVEGELRAQVSQNIKRLISIGCYRGMRHRQGLPVRGQRT
                     RTNARTRKGPRRTAGAKRGR"
     gene            complement(7974..8087)
                     /gene="rpmJ"
                     /locus_tag="PK016_03415"
     CDS             complement(7974..8087)
                     /gene="rpmJ"
                     /locus_tag="PK016_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HXL02152.1"
                     /translation="MKVSASVKPRCEKCKVIRRRGKVMVICSNPRHKQRQG"
     gene            complement(8104..8322)
                     /gene="infA"
                     /locus_tag="PK016_03420"
     CDS             complement(8104..8322)
                     /gene="infA"
                     /locus_tag="PK016_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012506584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HXL02153.1"
                     /translation="MGKEDIIEVKGTVLEALPNAMFKVELETGKVILAHISGKMRMHY
                     IRILPGDKVTVQISKYDLTKGRIVYRYK"
     gene            complement(8325..9083)
                     /gene="map"
                     /locus_tag="PK016_03425"
     CDS             complement(8325..9083)
                     /gene="map"
                     /locus_tag="PK016_03425"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HXL02154.1"
                     /translation="MSKLVSEDEIEKIRQSGKILAEVIREIKKLIIPGVKTSFLNQVA
                     ADLIKSHGARPAFLGYRGYPASICISLNEQVVHGIPGERIIKPGDLVSVDIGVEFQGF
                     YTDAAFSVIAGDGNPLAQKLVEVTEKSLYEGIKRALPGNRIGDISHAVQETIESAGFS
                     VVRDFVGHGVGRALHEEPQIPNFGKKNRGPVIEEGAVLAIEPMANEKGYQVEILDDDW
                     TVVTKDGGLSAHFEHTVWVTKKGPVILTRCGDEE"
     gene            complement(9080..9739)
                     /locus_tag="PK016_03430"
     CDS             complement(9080..9739)
                     /locus_tag="PK016_03430"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012940719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HXL02155.1"
                     /translation="MRIVLLGPPGAGKGTQAKMMEKDLGISTLSTGDIIRLAIKNQTP
                     WGKKAEEFVKSGRLVPDDVVIGIVKEKIDQDNLWSGFILDGFPRTIKQAEALEEMLKE
                     KNLSLDVVLYFAVDKEEIIKRLSSRRVCENCQTPYNLLSNPPHQDEVCDRCGGKLVQR
                     DDDKPEVIRKRLDTYEEETKPLIDFYEKRNLLQVIPSSAPIEEVYDRVKEVLKEVMAK
                     Q"
     gene            complement(9756..11024)
                     /gene="secY"
                     /locus_tag="PK016_03435"
     CDS             complement(9756..11024)
                     /gene="secY"
                     /locus_tag="PK016_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HXL02156.1"
                     /translation="MWESLLKLFRIPDLKRRILFTLSMLVVFRIGAHIPVPGIDPGAL
                     ANIFSQGGILGFLDMFAGGALSNFSILALGIVPYINASIIIQLLTAVIPRLEELSKSG
                     EEGRDKIAQYTRWFAVVLALVQGFGITSWIEGMGVVLYPSWGYKITVLLTLTAGTIFL
                     MWLGELISDWGVGNGTSLIIFAGIVARLLPQLVRTFSLIKVGEINLLYFILSLVMNVA
                     IIAVVVAFQEAQRRIPVQYSKRVVGRRMYGGQSTHIPIRVVQGGVIPIIFASSILLFP
                     ATLAQFFQGSEIMKSIANALSPNSPLYLSLYAALIIFFTYFYTAVSFNPMELADNMQK
                     YGGFIPGIRPGRPTVEYLDRTLSRITLWGALFLTFVAIVPNIITDLTKVTTFYFGGTA
                     ILIIVGVAIETVRQLEAQLLMRHYEGFIRK"
     gene            complement(11026..11466)
                     /gene="rplO"
                     /locus_tag="PK016_03440"
     CDS             complement(11026..11466)
                     /gene="rplO"
                     /locus_tag="PK016_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HXL02157.1"
                     /translation="MGVNELKPQDGSFHYRKRVGRGLSSGHGKTSGRGHKGQKSRSGS
                     GIPPYFEGGQMPLVRRVPKRGFRSWDRKEWQIINLEALNRFEEGEEVNKELLQKAGLI
                     KNLNLPVKVLARGELNKKLTVKADAFSLSAKEKIEEKGGRAEVV"
     gene            complement(11468..11668)
                     /gene="rpmD"
                     /locus_tag="PK016_03445"
     CDS             complement(11468..11668)
                     /gene="rpmD"
                     /locus_tag="PK016_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014194602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HXL02158.1"
                     /translation="MGEKLLKITLKKSPIGRPESHKRTVKALGLRKLNQTVYHKDSSS
                     LRGMVNKVAYLLEVESVEEGER"
     gene            complement(11682..12191)
                     /gene="rpsE"
                     /locus_tag="PK016_03450"
     CDS             complement(11682..12191)
                     /gene="rpsE"
                     /locus_tag="PK016_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HXL02159.1"
                     /translation="MRRVKPEGLELKERLIGVSRVSKVVKGGKRFGFRALVAVGNGEG
                     VVGIGTGKAKEVPEAVRKATERARKSLIQFPMRGTTITHELVGKAGASQVVLRPAAPG
                     TGVIAGGAVRAILEVAGIKDVLTKSLGSNNPVNLARATMQGLADLKDPREVARLRGKE
                     VKDFFTVSR"
     gene            complement(12209..12568)
                     /gene="rplR"
                     /locus_tag="PK016_03455"
     CDS             complement(12209..12568)
                     /gene="rplR"
                     /locus_tag="PK016_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HXL02160.1"
                     /translation="MSVEEKREKRDKRHRRVRKKIQGSAEYPRLAVFRSLRHIYGQLI
                     DDSEGKTLVAVSSLTSELKGKDGSKVEIAKEVGKLLAQKAREKGIETVVFDRGGYKYH
                     GRVKALAEGARECGLRF"
     gene            complement(12583..13119)
                     /gene="rplF"
                     /locus_tag="PK016_03460"
     CDS             complement(12583..13119)
                     /gene="rplF"
                     /locus_tag="PK016_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017434007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HXL02161.1"
                     /translation="MSRVGKKPIIIPDGVNVEIEGRTIVAKGPLGTLEREIHPRVKVE
                     KEGNVLQVIREGEGKLDRSLHGLTRALVANMVEGVSRGFEKALEISGLGYRAQKKGQN
                     LVLNLGFSHPMEVEPPPGITFEIEGQVVKVKGVDKEKVGQVAANIRKLRKVEPYHGTG
                     IKYVGEITRRKQGKATAK"
     gene            complement(13133..13534)
                     /gene="rpsH"
                     /locus_tag="PK016_03465"
     CDS             complement(13133..13534)
                     /gene="rpsH"
                     /locus_tag="PK016_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009306342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HXL02162.1"
                     /translation="MAHTDPIADMLTRVRNALKAGENTVKIPASRSKIEITRILKQEG
                     YINDYKVLAQEGGKSDLIIELKYGPNRERVINGLKRISKPGLRVYAGKNEIPVLLGGM
                     GTVVVSTSQGVMTGKEAKKLGVGGEVICYIW"
     gene            complement(13550..13735)
                     /locus_tag="PK016_03470"
     CDS             complement(13550..13735)
                     /locus_tag="PK016_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HXL02163.1"
                     /translation="MARKAKIEREKKDLKFKVRYRNRCSLCGRPRGYLRDFGLCRICF
                     RTLASKGEIPGVTKSSW"
     gene            complement(13749..14288)
                     /gene="rplE"
                     /locus_tag="PK016_03475"
     CDS             complement(13749..14288)
                     /gene="rplE"
                     /locus_tag="PK016_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015390349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HXL02164.1"
                     /translation="MSITKEKYQKEVIPRLVETFGYRNVMQVPKMEKIVINMGVGDAT
                     QNSRYLDLAVEELTIIAGQKPLVTRAKKSISNFKLRRGASIGCKVTLRKNLMYEFMDR
                     FLNIAVARIRDFRGFPDSSFDGRGNCTIGIKEQLIFPEISYDKVERVRGMNITFVTTA
                     STDEEAKALLEALGLPFRR"
     gene            complement(14306..14653)
                     /gene="rplX"
                     /locus_tag="PK016_03480"
     CDS             complement(14306..14653)
                     /gene="rplX"
                     /locus_tag="PK016_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HXL02165.1"
                     /translation="MESREKKNGAVIKKGDVVEIVRGKNRGKRGKVLKVFPQEGRVVV
                     EGINMVKKHTRPTQDNPQGGIINRENPIRISNLRLVCSRCNQLTKVQRVKVAESRFRV
                     RVCKKCGEIIDKV"
     gene            complement(14668..15036)
                     /gene="rplN"
                     /locus_tag="PK016_03485"
     CDS             complement(14668..15036)
                     /gene="rplN"
                     /locus_tag="PK016_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HXL02166.1"
                     /translation="MIQPRTMLEVADNTGAKKIMCIRVMGGSNRRYASIGDVIIAAVK
                     EAEPHSNVKKGEVVRAVVVRTRKEVGRSDGSFVRFDDNAAVLVTNQDVPRGTRIFGPV
                     GRELRDRNFSRIISLAPEVV"
     gene            complement(15011..15301)
                     /gene="rpsQ"
                     /locus_tag="PK016_03490"
     CDS             complement(15011..15301)
                     /gene="rpsQ"
                     /locus_tag="PK016_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HXL02167.1"
                     /translation="MGARKRLTGEVVSNAMDKTVVVKIVRISEHPLYGKKVKKVRRFP
                     AHDENKICAVGDRVVIEETRPLSKTKRWRVVTVVQKARVKGGEEIDSAENYA"
     gene            complement(15302..15514)
                     /gene="rpmC"
                     /locus_tag="PK016_03495"
     CDS             complement(15302..15514)
                     /gene="rpmC"
                     /locus_tag="PK016_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HXL02168.1"
                     /translation="MKTLELRNLTDEELNQRFKDLRTELFNLRFQSITGQLGNPKRIQ
                     AVKKDIARVQTVLRERELSKEKQGVK"
     gene            complement(15526..15936)
                     /gene="rplP"
                     /locus_tag="PK016_03500"
     CDS             complement(15526..15936)
                     /gene="rplP"
                     /locus_tag="PK016_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HXL02169.1"
                     /translation="MLMPNRVKYRKQHRGRMKGLAARGNKVDFGDFGLQALEPAWITN
                     PQIEAARVALSRHMKKGKIWIRIFPDKPYTKKPTETRMGKGKGPVEGWVAVVKPGRIL
                     FEIGGVDRDTAQEALRLASHKLPIKTRVVERSID"
     gene            complement(15920..16648)
                     /gene="rpsC"
                     /locus_tag="PK016_03505"
     CDS             complement(15920..16648)
                     /gene="rpsC"
                     /locus_tag="PK016_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HXL02170.1"
                     /translation="MGQKVNPIGLRLGIIKDWQSRWYAENKFKDYLIEDLKIRKEVKS
                     RFYHSGVSRVEVERLANQIKIIIKTSRPGIVIGRKGSEVEKLRQDIQSLTGNQNIQIS
                     VEEVSIPELDAQLVAENVASQIERRVSYRRAMKQAITRALRMGAQGIKISCSGRLAGA
                     EIAREEWYREGRLPLHTLRADIDYGFAESLTTYGRIGVKVWIFKGEVISPGELLERES
                     TAVLEKKQEEASYEEVAEDHVDAQ"
     gene            complement(16649..16990)
                     /gene="rplV"
                     /locus_tag="PK016_03510"
     CDS             complement(16649..16990)
                     /gene="rplV"
                     /locus_tag="PK016_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HXL02171.1"
                     /translation="MEVKATAKYLRISPRKARQVVDLIRGKSAQEALATLQFIPKKSA
                     RMVYKVLKSAVANAENNYNMDVDQLVVSQAFVDQGPTSKRVRPRAMGRGYLIRKRTSH
                     ITVAVSEKEEQ"
     gene            complement(17005..17289)
                     /gene="rpsS"
                     /locus_tag="PK016_03515"
     CDS             complement(17005..17289)
                     /gene="rpsS"
                     /locus_tag="PK016_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HXL02172.1"
                     /translation="MGRSTKKGPYVDLRLRERIEELNRKGEKRVVKTWCRSCMIIPDM
                     VGHTIAVHNGKKHIPVYVTEQMVGHKLGEFAPTRTFRGHGNPTERSTALK"
     gene            complement(17291..18115)
                     /gene="rplB"
                     /locus_tag="PK016_03520"
     CDS             complement(17291..18115)
                     /gene="rplB"
                     /locus_tag="PK016_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HXL02173.1"
                     /translation="MANLKEYKPVTPGRRHMSGCTFEEITPGGPEKSLLKPLKSKAGR
                     NNQGRISVRHQGGGHKRKYRCIDFKRDKFGIPARVERIEYDPNRSARIALLKYVDGEK
                     RYILAPLGVVVGDVLMSGEEADVRPGNALPLRNIPVGTFIHNLELRKGKGGQLARSAG
                     AYAQLMAKEGSYAQVRLSSGEVRLIHIDCLATIGQVGNIDHENVAIGKAGRKRWLGVR
                     PTVRGVAMNPIDHPHGGGEGKAHGGRHPVTPWGVPTKGFKTRKNKKTDKWIVKRRK"
     gene            complement(18131..18418)
                     /gene="rplW"
                     /locus_tag="PK016_03525"
     CDS             complement(18131..18418)
                     /gene="rplW"
                     /locus_tag="PK016_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HXL02174.1"
                     /translation="MNNKREILISPIITEKTVNLQKESKYIFKVDSRATKAEIKKAVE
                     DTFGVTVTKVNTVRVEGKTKRLGRFEGKSSSKKKAIVFLKPGEKIKAFDIT"
     gene            complement(18411..19037)
                     /gene="rplD"
                     /locus_tag="PK016_03530"
     CDS             complement(18411..19037)
                     /gene="rplD"
                     /locus_tag="PK016_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HXL02175.1"
                     /translation="MELKVWDKEGKVVDTITLEDIVLSDKDYHLLYLSVKAFLDNQRL
                     GTAKTKTRGEVRGGGKKPWRQKGTGRARQGSIRSPIWKGGGVTFGPVPRSYYHSLSRK
                     EREKALILALGEKINQGLLHLVEEIDFDTPQTKKAKQFLSPLVGDEFRKVLVVTGDNK
                     ENVRRAFSNLPDVKVKSAGELTAYFLVDSRVVVMEKKAFSLLWRQEYE"
     gene            complement(19056..19670)
                     /gene="rplC"
                     /locus_tag="PK016_03535"
     CDS             complement(19056..19670)
                     /gene="rplC"
                     /locus_tag="PK016_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HXL02176.1"
                     /translation="MTYGMIGEKVGMTRIFTPEGASIPVTVIKCGPCYVTGKKTEERD
                     GYQAWQVGYEEIKEKKLNKPRLGHLKKGGLPPLKFLKEFSFKDAQYQPGEVLKVDGFQ
                     VGDRVDVAGKSKGKGFAGVMKRLGYRGGEASHGSMFYRAPGSIGGTDAGRVFKGKGLP
                     GRMGGKRVTVRNLEVVGINAERDLLLLKGAVPGPAGSLLVIIRK"
     gene            complement(19706..20011)
                     /gene="rpsJ"
                     /locus_tag="PK016_03540"
     CDS             complement(19706..20011)
                     /gene="rpsJ"
                     /locus_tag="PK016_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HXL02177.1"
                     /translation="MAQKIRIKLKAYDHKILDQSALKIVQAVERTGAAVSGPVPLPTE
                     IAKYTVLRSPHVDKKSREQFEMRTHKRLIDIMDPNPKTVDALMHLDLPAGVDIEIKL"
BASE COUNT         5326 a         5233 c         3312 g         6213 t
ORIGIN      
        1 agcgtagtct tctcagagag aaaagccagc gtttcccatt gtggtctaag agagctctct
       61 ttcttcggtc ataaggtttt agttctccct ggcagtaagg acagtgaatg gtttcctccc
      121 ctgtcactag aaaaagtggt gagtgttatc ccattctttg gtatagtgtg atacaataat
      181 catacctttg gggggccttg gggaagttag ttatccatcc ttgaggtcct tctcctttcc
      241 tcagtatttt ccttaaagaa tttcccctta aagattatta cttggacatt atatatggca
      301 atttagagac agacaatctc ggtagctttt ggacatcctg ggttggtata aacaattggg
      361 tatctggtta ttgtccaatc ttccccatcc aaccggaaca tagaaggtaa aactcaagag
      421 atctgctccc ttcgatatag catcaaaata ttttgctagg gcactgggat cagttatgtc
      481 aaggccggcg tataattgac ccactttggc cggtttaaaa ttgacccatc tcctggtttt
      541 caacctgatg aattagaatt aggtgtccca taaattccgg ctctgatcct atctttaagt
      601 cgataactgt taccccggat atttatcacc tgggcatggt ggagtaatct atccaatagg
      661 gcagtagcca gaatgggatc tcccatcacc tccccccatt cgccaaagcc tttgttactg
      721 gttatgatga tacttccccg ctcataacga ctactcacta actgaaatag taaagtagcc
      781 tctaagtggt ctaagggaag ataacccact tcatcgatga tgaaaagctt aggccggaga
      841 tggagactca agcacttatc caaccggttc tctttatagg ctcgttttaa atcctctagg
      901 agatgactta agctggtaaa gtagacacct accccctgta agagagcttc tactcctaag
      961 gccactgcta agtgcgactt accgttatgg aaagctttcc ataacggtaa aagccatctg
     1021 gcgactgcta taggagtaga agcctgtaat caaggtcaaa gggtaaaatt cttctctact
     1081 gcttctcttg taaatcagct taccgatctc aaagagaggg gggaactgag gaagtttatg
     1141 aaccagatag agaaatggga cctcttgatt tgtgatgagt ggggttatgt acctttggag
     1201 aaaggaggtg cccaattact ttttcaggtg atatcggaat gctatgaaaa acgcagtatc
     1261 attattacta ccaatctgga attcagcaag tggaatggaa tattttatga tgagagaatg
     1321 agagctgcca tcattgaccg tttaattcac cacagtcatt tactggtctt ttccggaccc
     1381 agttaccggt tgactcattc tactatgaac tgttaattct ggaattaagg gtgctgcaaa
     1441 aattaacttc cggaatgctg caaaaattaa cttccagaat gctgcaaatt cctcttgcca
     1501 aatacatcct acaatgagtt gacactatca ggtcttttcc tccgtgttat atttgtcttt
     1561 catattttgt ttttgtgttt ttctcttttt tggaggttat gagggggcaa gcccccttat
     1621 tgttctatct aatttgtttt ttgtttacgc cgtagactgt cttttctttt ctctgtgctc
     1681 ttttgttttt taagttttct taacttacgt cgcagactgc tcttttccca gcgtactggc
     1741 ggagcttttt ccaccagtaa tctttgctta tggttctacc tcaataaggg tgaaacttta
     1801 gccctataat tttcatcacc tatttatcct cagaactata ctttcttttc cctccagagg
     1861 ggaactatcc atccctcatc caccagctgt aaattctggt agggattaaa atttggagga
     1921 tagcaaacct gcatcaaaaa taatccccgg gaaggaagaa ccaatcggtt aaaggaacga
     1981 gaagggtgtt ccaacataaa ttttatctct tccggctttt tcttccccat ccccactttc
     2041 cacagctctc cagcaataat tctcaccata ttgtataaaa acccattccc ttccacccac
     2101 aggcgcagaa gcccccggtg cttctcttct atctgaatag aataaatagt ccgaactgat
     2161 gttcctactc ctttcgagga gctaaaagag gtaaaatcat aagtcccttt aagaagggat
     2221 aggcattctt tcactaaatc taaattaaaa tcccggggag agaatgaata tacaaaaggc
     2281 tgccaaaaaa ccggagaaag cgaaggagag aaaacgtaaa catagatttt acgaaccgca
     2341 ctctttcgag gatgaaaaga aagaggagca tctaaaatat caactactct aatatcaggg
     2401 ggaagtagag cattaaatgc tttttttata gcttctattg aaagatgagt agaagtggta
     2461 aaagaagcaa tctgccctgt agcatgaact ccagcatcag ttctaccaga agcataaaga
     2521 gtgattttct cctggaggat tctttctccc accccctcta ttattccctg aatagttttg
     2581 cccctgctct ggcgttgcca accctgatat cttgagccat catattctaa taccaaactc
     2641 aggttacgga gagacatcag ataaaagagt gaagggaaag gagaaagaca aaagaaaaag
     2701 ataaagccag aaaataataa tcagaagaag tgagctccaa aacccgcagg cgagttcttc
     2761 cttttcctcc tcgataacat cgagattcca tagcaatggc caactcatca gctcttttaa
     2821 aagaattaac aaaaagagga accaaaagag gaattaagtt tttagccctt tgtattatgc
     2881 ctcccgaatc caagttcata cctcgagcca tttgggcttt taaaatccga tctgcttctt
     2941 ctaaaagagt gggaataaaa cgaagagcaa tggtcatcat catagctaac tcgtgagctg
     3001 gaaatcccca tctttttaaa gggctcaaca aatactccat cccatcggtt agctctaccg
     3061 gtgaagtggt gagagtcaaa agagaagtaa ctaaaatcaa gaggcataac cgcgtcacga
     3121 tcaaaagtcc ttttgatacc ccctcccggg aaatgtgtaa aaaaccgtat tcccagatat
     3181 attccccagg agtgaaaaag aaatgtagca ccagagtaag aagaaggaga aatagaagag
     3241 gtctcaagct tctcaagaga taagcaaaag aaagatgaga aagagaaact aaaaatatta
     3301 aggctatccc ccacaacaga aaggaaaacc aatcttgtaa gataaaaagg tcgataatgg
     3361 aaatccctac gaatacaatt ttaacccggg ggtctaaacg gtgtatggga gaatccaatg
     3421 gaatatattg accaatgact aaacttctac cccacattgg ctttctttct ctccagaatt
     3481 ttaagctgga tttcttctgc cagttcctga ggggttaaaa ctcccggggt aacatcaaag
     3541 cccctctctc tcaaaagcca ggccacctgg gcagtcacag gaggaaaaat gcccgaactt
     3601 cttatccatt ctacttgaga gaaaacctgt cggatatcat catcaaattt tattttcccg
     3661 ctatccatta ttattatcct ttttgccact cgcgccaggt cttccatact gtgggaaacg
     3721 attataaggg tcaaacctct ctctttctgg agtttttcca gttgattcag tatgattctt
     3781 ttaccttccg ggtctaaacc agcagtaggt tcatccagaa ctaaagcctc gggctccata
     3841 gagaggatgc tggcaatggc tactcgtctc atctgtcctc ctgaaagttc aaaaggactt
     3901 ttatctttga tagcctcaaa attgagccct actgccccca aagcccatct tactttatat
     3961 tccagctctt tcccctccac ccctaagttg tgaggagcaa aagctacttc tttgaaaacc
     4021 gtctcttcaa aaaactggtc ttcgggatat tgaaaaacta gacctatttt acggcgaatt
     4081 tcctgtaaag gaactccctt tttgcctgta tccactccat ccaccagtac tctgccactc
     4141 tggggaatca aaagagcatt gaaatgctgg ataagagtag atttaccaca accagttcta
     4201 cccacaatgc caatactctc cccccgttca atttccaggt ctatgtcttc cagtgctgta
     4261 atagcaagag gcgtaccagg tagataagta aaagatactt tttctaattt tataaacaca
     4321 attcctgtac cagttctccg ggagaaagag gcagcacctc aaagagagaa ggaaattgct
     4381 tctttataag aagagcagtt tctactacct ggggaagttc caatccccat tcttctattt
     4441 tttctacctg agaaaatatt tctcgcggtt tcccccgggc tactatcctt ccttcgtcca
     4501 taatcaatac ttcatctgct tcagtggctt cttccacatt atgggtgata tgaaaaacag
     4561 caatcctctc tcgcagattg ataatcacct tcatcagttc ctcccttcct tcggggtcga
     4621 gcatagaggt agcttcatcc agtattaaat actccggatg catggccaga actccagcaa
     4681 tagccactcg ctgcttttga cccccagaga gaaggtgagg ctctttatta cgatattccg
     4741 ccatacctac taagtttaga gcctcttcca cccgggcaag aatttcctcc ctgggtaaac
     4801 ctaaattttc cgggccaaaa gctacgtcct cttctactgt ggtagcaata atttgattat
     4861 cggggttctg aaaaaccaga cctacccggc gtctgatttc ccagatagat tcttcatccc
     4921 cggtgttcat cccatctact attacttccc cgcttttggg gagtaataag ccattagtat
     4981 gttttgccag agtagatttg cctgaaccat tgcgacctaa taaaaccata aatttaccct
     5041 ggggtatctc taaatctact tctctcaaaa cttcttcatt ccctacttct ggctcgtctt
     5101 cctccccata ccaaaagttt acctttttaa attctatcaa ttccttggta ctccagaggc
     5161 aacaaactat ttactctact atttctacca aaactaccgg agctgcatct ccctgacgat
     5221 atcccatctt taccaggcga caataacccc ctcttctttc tctaaaacga ggcgctattt
     5281 cccggaaaag gactttaaat gcttctttat ctctaagaca ggaaactact tctcgtagag
     5341 aagcattatc tccttttatt gccaggtaaa ccacccggtc aaaatatctc ttcagtgcct
     5401 tacctttcgc ttccgtagtt tgtatcttcc cgctttttat caaagaaact aacaggttag
     5461 acatcatgct ttttttatgc gccgtagtcc tccctaattt ttcagttctc cgccggtgcc
     5521 tcacttttat tcactccttt ttcctctctg ctttctgctt cagaactcca cttttgcact
     5581 ttttttacta tttcttcata ggttttttga cctaaattct tgattttttt caaatcttca
     5641 ggccgatttt ggagaacatt taccagttcc tgtaccgtat cgatatgagc ccttttaaga
     5701 caattgtaag ctcttactga aagctctagc tcttcaatac tggtttccgg ttcagccatt
     5761 tcctcgtctt cttcagcctc ttcagtcgat acattttccg ctatcttttc tttcaacagc
     5821 tccccaaaac gggagaactc tccagtcaaa aggtccagag actggctgag agcttgctgg
     5881 ggagtaatag ctccattagt ccatatttct ataatcagtt tatcataatt ggtaaactgc
     5941 cccacccggg tatccagaac ctggaaattt acttttctca ccggggtaaa caaagcatca
     6001 acaggaataa aatcgtaatc ctcttttatt tctcctcctt ccacctccag gtaacctcgt
     6061 ccccttttca ctgatatttt catagaaagc ctggcatcct catctatttc tgctaaatga
     6121 tggtctagat tgattatttc tacattgctg ttaggctgta tatcccgagc cgtaacttcc
     6181 cgcaattctc caggagtccc ctctacttct aaataaaggg tctcctcatc agaataagag
     6241 cgtaaaacca ggcccttaag atttagaatt atatcggtta tgtcttctct tactccaggt
     6301 agagaagaaa actcatggat taccccttca atttgtaccc cggtaactgc tgctccttct
     6361 atagaggaaa gtaaaacccg acgtatagcg ttacctaccg taatacccca acctacttcc
     6421 agaggctcga tagagaactt accatattga accttagtag tgtggtcttc ttcccagtct
     6481 aaaagatcac acctcgttat atttcgaata tccagcatta ccaacacctc aaaattattt
     6541 agaataaaat tccactatga gctgctcttc cactgcgtaa tcaatatctt ctctccgtgg
     6601 ggctcgcaat atagtagctt taccatcctc acccactccc agccaggcag gagcaggagt
     6661 tttttcctct tccattagct ccttaatttt ttgattttct ttcccccgag gagatatttc
     6721 caccacatca ccttcatcca ccaggtaaga tggtatatcc accttgcgcc cgttaactag
     6781 aaaatgacca tgcagcacca tttgcctggc atcagaacga gagtctgccc atcctgctcg
     6841 aaaaactacg ttgtccaatc ttctctctaa aagagaaaga aggttttctc ccgttactcc
     6901 aggaagacgg ttggcttttt caaaataatt tttaaactga ttttcagtta tgccatacat
     6961 aaatcgtacc cgctgttttt ccttgagctg caaaccataa ctggacaatc tccggcgagc
     7021 ccctttatgt actccaggcg gaaaaggccg cttctccata gcgcatttat cagtataaca
     7081 gcgaggtcct tttaagaaaa gtttgctccc atgtcttcga cagagtcggc atttagcatc
     7141 agtgtacctt gacattttct atatcctcct tcataccctt ctttgtttag gtggccggca
     7201 accattatga ggaataggag taacatcttt tatagaactg atgattaaac cagctgcctg
     7261 caaagaccga atggcagtct ctctccctga accaggacct tttaccagta cttctacttc
     7321 tcgcaaaccc tggtccatag ctttcttcgc tgcttgctct gcagctaact gagcagcaaa
     7381 aggagttcct ttcttcgttc ctttaaatcc actggctcca gcactggacc aggataaaac
     7441 attcccctgt ttatcagtta tggaaacaat ggtattatta aaagtagact ttatatgagc
     7501 aataccttcc ctgaccgttc ttttttctct tttctttttc ctacctgctt tagccaaatt
     7561 aatctaaacc tccctttatc atacttttgt aattatctac ctctttttgc tcctgcagtc
     7621 cttctaggac ctttacgagt tcgggcatta gtacgggttc tctgccctct caccggtagt
     7681 ccttgtctat gccgcatacc ccggtaacat ccaatggaaa tcaagcgctt gatgttttga
     7741 gaaacctgag ctcttagctc tccttccacc ggatattttt ctaccacttt ttgaattcgt
     7801 gctaactcat cgtcacttaa atccttaact ctgattccgg ggtctacttt agtctcctct
     7861 aaaatttttg ctgccaactt cctgcccact ccataaatat aagtaagcgc aatatctatc
     7921 cttttatggt tgggcaaatc aactcctgca attctggcca taagacaaac ctcctaaccc
     7981 tgtctctgct tatgccgggg attagaacag ataaccatta cttttccccg ccgacgtatc
     8041 actttacatt tttcacatct cggtttcacc gaagcactaa ccttcatctt cttacctcct
     8101 tattcactta taccgatata caatccgccc tttagtaagg tcatattttg atatctgtac
     8161 ggtaacttta tcaccaggaa ggattctaat ataatgcatt ctcattttac ctgaaatatg
     8221 agctaatatc actttacccg tttccagttc tactttaaac atagcattgg gtaaagcttc
     8281 taaaactgtc ccctttactt caataatatc ttccttaccc attattactc ttcatccccg
     8341 caacgggtta aaatcaccgg gcccttttta gtaacccaga cggtatgttc aaaatgagct
     8401 gataggccac catctttagt taccaccgtc caatcatcat ccagtatttc tacctggtat
     8461 cctttttcat tagccatagg ttcaatagct aacactgcac cctcctcaat aactggccct
     8521 cgattctttt ttccaaaatt aggtatttga ggttcctcgt gtagagctct acctacacca
     8581 tgcccaacaa agtctcttac cactgagaaa cctgcagatt caatagtttc ttgaacggca
     8641 tgagatatat caccaattct attaccaggt aaagcccttt ttattccctc atatagagat
     8701 ttttccgtta cttctaccag cttttgagcc agagggttac catcacctgc tatcacactg
     8761 aaagcagcat cagtataaaa cccttgaaac tccactccta tatccacact cactaaatct
     8821 ccaggcttga ttatccgttc tccaggaata ccatgcacca cctgctcatt aagagaaata
     8881 caaatagaag ccggataacc tcgataacct aaaaaagctg gtcgagctcc atggcttttt
     8941 attaagtctg cagctacctg attcagaaaa gaagttttaa ctcccggaat tatcagcttt
     9001 tttatttccc tgataacctc ggctaaaatc tttccactct ggcgaatttt ttcaatttca
     9061 tcttcactca ctaacttact cattgtttgg ccatcacttc cttcaaaacc tctttaactc
     9121 tatcatacac ttcttctata ggagcactgg atggtataac ctgcaataag tttcgtttct
     9181 cataaaaatc aattaaaggt ttagtttctt cttcgtaggt atccagccgc ttgcgaatta
     9241 cttccggttt atcatcatct ctctgtacca gctttccgcc acatcggtcg caaacctcat
     9301 cttgatgagg aggattagaa aggagattat aaggagtttg acagttctca caaacccgac
     9361 gagaggaaag tctctttatt atttcctctt tatccaccgc aaaataaaga accacatcta
     9421 atgatagatt cttttctttg agcatttcct ccagagcttc tgcctgcttt atggtgcgag
     9481 gaaaaccatc cagaataaaa ccagaccata aattatcctg gtcaattttc tctttaacta
     9541 ttcctattac cacatcatca ggaactaatc gcccgctctt tacaaattcc tcggcttttt
     9601 taccccaggg agtttgattt tttatagcca ggcgaattat atcaccggtg gacagggtgc
     9661 taatacctaa gtctttttcc atcatcttag cctgtgttcc cttacctgct ccagggggtc
     9721 ccaataaaac tatgcgcaat ttcccttctc ctttctcatt tacgtataaa accttcataa
     9781 tgtctcatga gtagctgagc ttccaattgt ctcactgtct caatggctac tcctactata
     9841 atgagtattg ccgttcctcc gaagtaaaaa gtggtaacct tagtcagatc agttatgatg
     9901 ttgggaacta tagcaacaaa agttaaaaat aaagctcccc acaaggtaat acgggagaga
     9961 gtacggtcta aatattccac tgttggtctt cccggtctta tccccggaat aaaaccacca
    10021 tatttttgca tgttatcagc cagctccata gggttaaaag aaactgcagt ataaaaatag
    10081 gtgaaaaata tgatcaaagc cgcataaaga gaaagataca aaggagaatt aggagaaaga
    10141 gcgttagcta tgcttttcat aatctccgac ccttggaaaa actgagctaa ggtagcagga
    10201 aaaagaagaa tagaagaagc aaaaatgata gggatcactc ccccttgcac cactctaata
    10261 ggtatatgag tactctgccc tccatacatc cttcttccca ccactctttt agagtattgg
    10321 actggaattc tcctctgcgc ttcctgaaaa gctactacca cagcaatgat tgctacattc
    10381 atcaccaggg acagaataaa atacagaaga ttgatttctc ctactttaat taaagaaaaa
    10441 gtcctcacca attgaggaag tagccgagct acgataccag caaagataat gagagaagta
    10501 ccgttaccca ctccccaatc agagatcaat tctcccaacc acatcagaaa tatggtcccg
    10561 gcagtaagag taagaagaac agttattttg tatccccagc tcgggtaaag aaccactccc
    10621 ataccttcta tccaggaggt tattccaaac ccttgtacca gagccaaaac aacagcaaac
    10681 catctcgtat actgagcaat tttatctctt ccctcttctc cactcttgct taattcctct
    10741 aaacgaggga tgaccgcagt tagaagctga atgataatag aagcattaat atagggaacg
    10801 atacccagag ctaagataga aaagttactc aaggcaccac cggcaaacat atccagaaaa
    10861 cccaaaattc ctccctgaga aaaaatattg gctaaagctc cggggtcaat tcccggaaca
    10921 gggatatgag caccaatgcg aaaaaccacc agcatagaga gagtaaaaag aattctcctt
    10981 ttcaggtctg gtattcggaa taatttcagc agagattccc acatcttata ctacctctgc
    11041 tcttccgcct ttttcttcga tcttctcttt agccgaaaga gaaaaggcat ccgctttcac
    11101 cgttaacttt ttattcaact ctccccgggc taaaacctta accggaaggt tcaggttctt
    11161 gatcaaccct gccttttgta aaagctcttt gtttacttct tctccttctt caaaacgatt
    11221 aagagcctcc aggttgatta tctgccattc ctttctatcc cagctccgaa aacccctttt
    11281 aggaacccga cgcacgagcg gcatctgtcc cccttcaaag taaggaggga ttccgctccc
    11341 cgagcgagat ttctgccctt tatgtcctcg tccgctggtc ttaccatggc ctgaacttaa
    11401 gcctcgaccc actctttttc gataatggaa agagccgtcc tgaggtttta attcgttaac
    11461 tcccatatta tctttctcct tcctctacgc tttctacttc taaaagataa gctaccttgt
    11521 taaccatacc tcgcaaggag gaagagtcct tatgatacac agtttgattt aacttgcgca
    11581 gacccagagc tttaactgtt cttttgtgag actccggtct accaatggga cttttcttta
    11641 atgtaatctt cagtaatttc tcccccattt tattctctcc ttcagcgaga aacagtaaag
    11701 aagtctttta cttctttccc ccgcagccgg gccacttccc gagggtcttt caaatctgcg
    11761 agtccttgca tagtcgctcg cgctaaattt acaggattat tgcttcccag agatttagtc
    11821 aacacatcct ttatccctgc tacttctaga attgctctca ccgctccgcc agcaataact
    11881 ccagtccccg gggcggcagg tctcaaaacc acctggctag ctccagcttt tcccaccaat
    11941 tcatgagtga tggtagtacc tctcatagga aactgaatta aagattttcg agctctctca
    12001 gtggcttttc tcactgcttc gggaacttct ttagctttac ctgtaccaat gcctaccact
    12061 ccttctccat ttcccaccgc cacgagagcc cggaaaccaa aacgcttacc ccctttaacc
    12121 actttgctaa ctcggctcac tcctatcaat ctttctttaa gctctaaacc ttctggcttt
    12181 actctcctca tctttcaacc tccatctgtt aaaatcgcaa tccgcattct ctggctcctt
    12241 cagctaaagc tttaacccga ccgtgatatt tatatcctcc ccgatcaaaa accacggttt
    12301 ctattccctt ctcccgggct ttctgagcca ggagtttccc tacctctttg gctatctcaa
    12361 ccttagagcc atctttacct ttaagttctg aagtaaggga agaaacagcc accagagtct
    12421 taccctcact atcatcaata agctgtccat atatatgcct gaggctgcgg aaaacggcaa
    12481 ggcgagggta ctccgcgcta ccctgtattt ttttccgtac tcttctatgt cttttatctc
    12541 ttttctctct cttttcttct accgacatat aagactctcc cttcacttcg cagttgcttt
    12601 accctgcttt cttctcgtga tttcacccac gtacttaatt cctgtaccat gataaggctc
    12661 tacttttctt agtttcctga tattagctgc cacctggcct actttttctt tatcaacacc
    12721 tttcactttc actacctgcc cttctatttc aaaggtaata ccagggggag gctctacttc
    12781 cataggatga gaaaatccca ggttaagaac taaattttgc ccttttttct gagctcgata
    12841 gcctaatccg ctaatctcca gcgccttttc aaacccccgg gacactcctt ccaccatatt
    12901 cgccaccaga gcccgggtta aaccatgaag agagcgatct aatttaccct ctccttctct
    12961 aattacttgg agaacatttc cttctttctc cactttcact cgaggatgaa tttccctctc
    13021 taaggtgccc agaggacctt tagccactat cgtgcggccc tcaatttcta cgtttacacc
    13081 atctggtatt attatcggtt tttttcctac acgggacata gctaatcctc cttcaccaaa
    13141 tgtagcaaat aacttccccg cctacaccta atttcttagc ttcttttccc gtcatcacac
    13201 cctgagaagt agagaccacc accgtcccca ttccccccag aagcacgggt atctcatttt
    13261 ttccagcata tactctcaag cctggcttgc ttattctttt caaaccattg attactctct
    13321 ctcgattagg accatatttt agctcaatga ttaaatcact cttcccacct tcctgggcaa
    13381 ggactttata atcattaata taaccttcct gtttaagaat ccgagttatt tcaattttag
    13441 agcgagaagc aggaatttta actgtattct ctcctgcttt cagggcattt cttaccctcg
    13501 tcaacatatc agcaatgggg tctgtatgag ccactatgct tttcctcctt taccagctcg
    13561 acttagttac tccaggtatt tcacccttgc tcgctaaagt gcgaaaacaa attctgcaca
    13621 ggccaaaatc ccgcaagtag ccccgaggtc ttccacacaa actacacctg tttctatagc
    13681 gtaccttaaa ttttaaatcc tttttttctc tttctatctt agctttacga gccattaaaa
    13741 aacctcctct acctcctgaa tggtaaaccc agtgcttcca acaaagcctt agcttcttca
    13801 tcggtggaag cagtagttac aaaagtgata ttcatgcccc tgacccgttc cactttatca
    13861 tagctaatct cagggaaaat caattgttcc ttgataccaa tggtgcaatt tcctctccca
    13921 tcaaaagaac tatccggaaa tcccctgaaa tcacgaatac gagccacagc tatattcaga
    13981 aaccggtcca tgaactcata cattaaattc tttctcaaag taaccttaca accaatagat
    14041 gctccccggc gtagtttaaa gttagatata gactttttag ctcgagtcac caatggtttc
    14101 tgcccggcaa taatagtcaa ttcttctact gctaaatcaa ggtagcgaga attttgagta
    14161 gcatctccta cccccatatt gatgactatt ttttccattt taggcacttg cataacattc
    14221 cggtaaccaa aagtctctac gagacgggga attacttctt tttggtattt ttccttggta
    14281 atggacacag ccacaggcct ccttgctaca ccttatctat gatttctccg cattttttac
    14341 ataccctgac tcgaaaacga gactcagcca ctttaactct ctgaacttta gtaagttggt
    14401 tgcagcggct acaaaccaac cttaaattag agattcttat ggggttttct ctgtttatta
    14461 ttccaccctg ggggttatcc tgagtaggac gagtatgttt tttaaccata ttaattccct
    14521 ctactactac tcttccctcc tggggaaaaa cttttaaaac tttccctctc ttcccccggt
    14581 ttttaccccg aacaatttcc accacgtcgc ctttttttat aactgcacca ttttttttct
    14641 ctcggctttc catcttaacc accttcctca cactacctca ggagccagtg agataatacg
    14701 cgaaaaatta cggtctctca attctcttcc caccggacca aaaattcgag ttcctcgagg
    14761 cacatcttga ttggtgacta aaacagcagc attatcatca aaacggacaa agctaccatc
    14821 tgaccttcct acttcttttc tggttcttac cactactgct ctcaccactt ctcctttttt
    14881 cacattggaa tgaggctctg cttctttcac cgcagcaata atcacatccc caatacttgc
    14941 ataacgacga ttagagccac ccatcacccg gatgcacatt atttttttag ctcctgtatt
    15001 atcagctacc tcaagcatag ttctcggctg aatcaatttc ttctcctccc tttaccctgg
    15061 ctttctgcac caccgtaact actctccatc ttttagtctt gctcaagggc ctggtttcct
    15121 caatcacaac ccgatctccc actgcacaga ttttattttc atcatgagca ggaaatcggc
    15181 gtactttttt aaccttcttt ccataaagag ggtgttcaga aattctcact attttaacca
    15241 ctactgtttt atccatagcg ttactaacca cttcaccagt taatcttttt cttgctccca
    15301 ttcacttaac cccctgcttt tctttagaca attctctttc tctcaaaacc gtttgtactc
    15361 gagcaatgtc ttttttaacc gcctgtatcc ttttaggatt acccaactgt ccagtaatgc
    15421 tctgaaaacg caaattgaaa agttcagtcc gcaaatcttt gaatctttga tttaactcct
    15481 catcagtcaa attacgaagc tctaaagtct tcatatctct cactcctaat caatacttct
    15541 ctccaccacc ctggtcttga tcggcaattt gtgggaagcc aatcggagag cttcctgagc
    15601 tgtatctcta tctactcccc ctatctcaaa aagaatcctt cccggcttca ctaccgctac
    15661 ccatccctct accggtccct ttcctttacc cattcttgtt tccgtaggct ttttagtata
    15721 aggcttatca ggaaagatgc gaatccatat ttttcctttc ttcatatgac gggaaagagc
    15781 tactcgagct gcctcaatct ggggattagt aatccaggcc ggctctaaag cctgaaggcc
    15841 aaaatctcca aaatcaactt tattaccccg agcagctaac cctttcattc tccctcgatg
    15901 ctgttttcta tatttaactc tattgggcat caacatgatc ttctgctacc tcctcataac
    15961 ttgcttcttc ctgctttttc tctaaaacag cagtactctc tctttctaac agctctccag
    16021 gagaaatcac ctctcctttg aatatccata ctttaactcc gattctcccg taagtggtga
    16081 gagattcagc aaaaccataa tctatgtccg cccgtaaggt gtggagagga agacgacctt
    16141 cccgatacca ctcttcccga gcaatttctg ctccagctaa acgaccggaa caggatatct
    16201 ttataccctg agcacccatc cttaaagccc gagtaatggc ctgtttcata gctcgccgat
    16261 aagaaactct ccgctctatc tgggaagcaa cgttttcggc taccagttga gcatcaagtt
    16321 cagggataga tacttcttct actgaaattt ggatgttttg attcccagtc aaactttgga
    16381 tatcttgacg aagtttttct acttcactac cttttctacc aataacaata ccaggccggg
    16441 aggttttaat aattatcttt atctggttag ccagtcgctc tacttccacc cgggagacac
    16501 cagaatgata aaatctactt ttaacttcct tgcgaatctt caagtcttct atcaggtaat
    16561 ctttaaactt attctctgca taccagcgag attgccaatc cttaataatt cccaaacgta
    16621 acccaattgg atttactttc tgccccaatt actgttcctc cttttccgaa actgccactg
    16681 taatatgaga ggtccttttt ctgatgagat accctctccc catagcccga ggacgtactc
    16741 gttttgaagt cggtccctgg tctacaaaag cttgagatac tactaactga tccacatcca
    16801 tattatagtt attctctgca ttagccaccg cagacttcaa aaccttatat accattcggg
    16861 ctgatttctt aggaataaac tgtaaagtag ccagagcttc ctgtgccgat tttcctcgaa
    16921 tcaaatcaac cacttgcctt gctttacgag gcgagatacg taagtatttt gccgttgctt
    16981 tcacttccat atttccatcc tccctcactt caacgccgta gagcgttccg tgggattacc
    17041 atgccctcta aaagttcgag taggagcaaa ctctcctaac ttgtgtccta ccatttgttc
    17101 agtaacataa acaggaatgt gcttctttcc attgtgaaca gcaatagtat gtcctaccat
    17161 atcaggaatt atcatacagg accggcacca ggttttaact accctttttt ctccctttcg
    17221 gttaagctct tcaattcttt ctcgcaaacg caaatctaca taaggaccct tttttgtaga
    17281 tctacccatc ctatttcctc ctcttaacaa tccatttatc ggtcttctta ttctttctgg
    17341 ttttaaaacc tttagtaggc actccccaag gggttaccgg atgtcttcct ccatgagctt
    17401 tcccttctcc tccgccatga ggatgatcga tagggttcat agccactcct cgtaccgtag
    17461 gtctcactcc cagccatctt tttcttcctg ctttaccaat agccacgttc tcatggtcaa
    17521 tgttacctac ctgacctatg gtagctaaac aatctatatg gatcaggcgc acttccccag
    17581 aggaaagtcg aacctgagca taacttcctt ccttggccat taactgggca taggcacctg
    17641 ccgaccttgc taactgccca cctttgcctt tcctgagctc cagattatga atgaaagtcc
    17701 ccaccggtat attccggaga ggtaaggcat taccagggcg aacatcggct tcttcaccgc
    17761 tcatcaaaac atctcccact acaactccta aaggagcaag aatatagcgc ttttctccat
    17821 caacatattt taaaagagca atacgagcag agcgattggg gtcatattca attctctcca
    17881 ctcgagctgg aatgccaaac ttatctcttt tgaaatcaat gcaccgatat tttctcttgt
    17941 gacctccccc ttgatgcctt actgaaatcc ttccctggtt atttctccct gctttacttt
    18001 tcagaggctt caaaagagat ttttctggcc caccgggagt aatttcttca aaagtgcaac
    18061 cactcatatg tcttcggcct ggtgtaactg gcttgtattc tttcaaatta gccatatcgc
    18121 tctctccttt ttaagtgata tcaaaggctt ttattttttc tcctggcttt aagaaaacta
    18181 ttgctttctt tttcgaacta cttttaccct caaagcggcc cagccttttt gtttttcctt
    18241 ccactcttac cgtgttcact ttggttactg ttaccccgaa ggtatcctct accgccttct
    18301 ttatctctgc cttagtggct cgggagtcca ctttaaagat atatttactc tccttctgga
    18361 ggtttaccgt cttctcagta ataatgggac taattaatat ctctcgctta ttattcatat
    18421 tcctgcctcc aaagcaagga gaaggctttt ttctccatca ccactactct tgagtccacc
    18481 agaaagtaag cagttagctc accagctgat ttgactttaa cgtcagggag attggagaaa
    18541 gctcttctca cattttcttt gttatcccca gtcaccacca gcactttcct gaactcatcc
    18601 cctaccagag gagaaaggaa ctgcttcgcc tttttggttt gaggagtatc aaagtcaatc
    18661 tcttccacca gatgcaataa tccctgattt attttttctc ctaaagctaa aattaaagcc
    18721 ttttctcttt ccttccgaga aagactgtgg taataagagc gaggaacagg accgaacgtc
    18781 actcctcccc ctttccagat aggagagcga atgcttccct gccttgctcg gccagtccct
    18841 ttctgcctcc agggtttttt ccctcctcct cttacttctc ctcgggtttt agttttggct
    18901 gtccccagtc tttgattatc caaaaaagct ttaaccgaaa ggtaaaggag gtgataatct
    18961 ttatcggaca ggactatatc ttctaaggta atagtgtcta ctacctttcc ttctttatcc
    19021 cacactttta gctccatctt ttatccccct attggttatt ttcgaattat taccaggaga
    19081 cttcctgctg ggccaggaac tgctcctttt aaaaggagca ggtctctttc tgcgtttatt
    19141 cccaccactt ccagattacg aactgttact ctctttcctc ccattctacc aggaaggcct
    19201 tttcctttaa aaacccttcc ggcatctgtg ccacctatgg agccaggcgc tctataaaac
    19261 atagaaccat gagaggcttc tccaccacgg tagcccagcc tcttcatcac tccagcaaat
    19321 cctttacctt tggatttacc agccacatcc acccggtctc ctacctgaaa gccatctact
    19381 ttcaacactt cacctggctg atattgagca tctttaaaag aaaactcttt caaaaattta
    19441 agaggaggaa gtccaccttt tttgagatgt cccagacgag gtttattgag ttttttttct
    19501 ttaatttctt cataacctac ctgccaggcc tggtatccat ctcgctcctc ggtcttttta
    19561 ccagtaacat agcaaggacc acatttaatt accgttaccg gaatagatgc tccttccggg
    19621 gtaaagatcc tggtcattcc tactttttct cctatcattc cataagtcac tgttttcatc
    19681 tgtatctcct tccccaatca actcatcaaa gcttaatctc tatgtctacc ccagctggta
    19741 aatcaagatg catcaaagca tcaaccgttt tagggttggg gtccatgata tcaatcaatc
    19801 ttttatgagt tcgcatttca aattgctctc gagatttttt atctacatga ggagaccgta
    19861 aaacagtata tttagcaatc tcggtaggta aaggtacagg tcccgagact gctgctccgg
    19921 ttctctccac tgcctgcact atttttagcg ccgactgatc cagaatttta tgatcataag
    19981 cctttaactt tattcttatt ttttgagcca ctttttacgc ctccctctcc ctattcaatg
    20041 atatcagcaa ctactcctgc tcctactgtt cttcctccct cacg
//
LOCUS       DAOSVE010000025        19287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_1823_length_19287_cov_67.689736, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000025 DAOSVE010000000
VERSION     DAOSVE010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 19287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19287
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1823_length_19287_cov_67.689736"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..719
                     /locus_tag="PK016_03545"
     CDS             78..719
                     /locus_tag="PK016_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HXL02178.1"
                     /translation="MAEILDQIAKELFAGNAKAVAELTQEALSKGFSPQEILNEGLIK
                     GMNEVGMKFKANEIYVPEVLIAARAMKAGMEILKPKLAETGVEPVGKMIIGTVKGDLH
                     DIGKNLVSMMMEGAGFEVIDLGIDVPAEKFIQAVKKYQPQLAGMSALLTTTMIQIREN
                     IKAFQEAGIRDKVKIMIGGAPVTQKFADEVGADGYAPDAASAVDKAKELLGIS"
     gene            1083..2777
                     /locus_tag="PK016_03550"
     CDS             1083..2777
                     /locus_tag="PK016_03550"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HXL02179.1"
                     /translation="MLSDLEIAQRAQMKPIVEIAESLGLSPDDIDLYGKYKAKVSLEV
                     LKNLSEKPQGKYIDVTAITPTPLGEGKTVTTIGLSQALNKIGKKSIVCIRQPSLGPVF
                     GIKGGAAGGGYSQVVPMEDFNLHLTGDTHAVATAHNLLAAFIDNHLHHGNKLNIDPFT
                     ISWPRVVDISDRALRKIVIGLGGKTNGVPRESSFDIAVASEVMAILALTTDIFDLRAR
                     LGRIVIGYDNNKKPVTAEDLHCAGAMTVLMKEAIKPNLLQTLENTPCFVHAGPFANIA
                     HGNSSIIADQIALRLSDYVVTESGFGADCGMEKFMDIKCRYSGLIPNCVVMVTSVRAL
                     KMHSGKYKVVAGKPLDTGLTCEDIEAVEKGAENMVKQIENARLFGVPVVVAINLFASD
                     TDREVETVGKIALENGAFAVEVSEVWEKGGEGGKDLAEAVVRACNEPQNFHFLYPLDI
                     PIKEKIEIIATQIYGADGVTYEPKAEAGIKRFTELGWDKLPICMAKTHLSLSHDPNLK
                     GRPRGFKVPVRDIRPSIGAGFLYPLCGEMSTMPGLPSEPAGNKVDIDGEGKVVGLF"
     gene            2789..3766
                     /locus_tag="PK016_03555"
     CDS             2789..3766
                     /locus_tag="PK016_03555"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXL02180.1"
                     /translation="MNYDLVIIGGGVAGLTLAQTLQDKLEVLVVEESSLGGMASKLGC
                     KATDECLSCGVCRFINVERELCFKKFDIWEGRKITSVDKSGRGFHLKTEKGEKVTSQL
                     LAVATGASPLEIKNLFHLGGGLPRVYSGWDVERGLNEGIISSFRDFSSLAFIQCAGSR
                     NFKEKRGYCSQVCCRYALRIAENLKFSFPSMDIDFYYMDLQILGKKKEKLWEIAGKIN
                     LIRHLPYEVKKEGGKLSLVFEEQGVERKNYDAIVLSVGMVPSSGTKEIGKWLGLNFDS
                     AGFIKSYGLGHTSREGVFVCGTACGPKDIETTQEEAQKVGEEIWRVRRG"
     gene            3768..5174
                     /locus_tag="PK016_03560"
     CDS             3768..5174
                     /locus_tag="PK016_03560"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXL02181.1"
                     /translation="MNKLLWLANEAFSFPDFWIEEVERNNDWEIEVKKWGDKLGSFTE
                     YDRIFILGDSFSFTQEKISLLSEEWEVNPLKVRVFPGEEVKIIFPESFLSILEQFIIQ
                     SLSQPLFPVEARRVVPENRVLVLPQLSPTLEQTLKKRGIDIIVPPSSFYLRREGINFR
                     LYPSRDNVGAIVVSPLEEKKFPASFPCEVQDTRRLVDIEFMEKEIEKKDFRKETIAFI
                     IPKKGVAEEEWRKAFRLSQILVERDKAEVFVLVEEVLVAGENLEKEYRKARSSGVIFE
                     KVDFSKLGTRPTLDMRGIEVEFTTERDKLRREVKADWLVLLPERNILPFDLAPFFCGD
                     KIGGEILPLENPNVLPFSSGIEGIFLSPPGEEEELSLVIKKYLTEGVVREIGRGEVDE
                     EKCVLCLTCLRTCPWEAIEIEGERKKARINEERCHLCGLCASFCPAHAIEIKGLSPEN
                     WVSAVSLGGKNESSSFRL"
     gene            5149..5511
                     /locus_tag="PK016_03565"
     CDS             5149..5511
                     /locus_tag="PK016_03565"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HXL02182.1"
                     /translation="MRVVALGCDKSGYPALLRWGEEDSLPFDEVKIFSLPCLGLVTEE
                     VIFRILENSDALLLAGCPLDSCHNQKGSQGAYLKVQRVNSLLQEADISKKVAIAFVTS
                     GKVGEIEKVSEELLWREK"
     gene            5508..6158
                     /locus_tag="PK016_03570"
     CDS             5508..6158
                     /locus_tag="PK016_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HXL02183.1"
                     /translation="MIIAERKPIKEILSFLGPGEKILVLGCGTCVTVCMSGGEKESRE
                     LSSLLRLKGWEAKDLTVERQCEPEFNLRVREEIAKVDTMISLACGVGVQALAECFPGK
                     KIFPGLNTTFMGMPKEQGLWEERCWGCGNCIVHLFGYICPITRCAKSLLNGPCGGSKE
                     GKCEVKPDRDCAWQLIYDRLKRENRLEVLYALQPPRDWSTSRYGGQRKMIREDVRL"
     gene            6155..7066
                     /locus_tag="PK016_03575"
     CDS             6155..7066
                     /locus_tag="PK016_03575"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HXL02184.1"
                     /translation="MSSLEEILRRGEFAVTAEVGPPKGTNREVIEKKCAELQGFVDAV
                     NITDNQTAIVRLSSFASCLIAKDKGIEPVMQMVCRDRNRIALQSDFLGACAWGVENFL
                     CLTGDHQSMGNHPQSKNVFDVDSIQLLQIFKNMRDEKVFQNGEPLKGEIKAFLGAGEN
                     PFADPLEFRVIRLAKKISAGAQFIQTQAVFDLEIFSRFMEEVRKMGLDKKAFILAGVI
                     PVKSLAALNYMKNEVPGLVVPDYLVERMREAKDPKEEGVKICLETIEQLKKIEGVRGV
                     HIMAISWESIVPDIVKGANLYPRSGRS"
     gene            7071..7556
                     /locus_tag="PK016_03580"
     CDS             7071..7556
                     /locus_tag="PK016_03580"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXL02185.1"
                     /translation="MAVDYQFKYQVANRLGGEGLLVCFSCGICTAGCPVAEVEGGFNP
                     RRIIHEVLLGEREKVLRSKFIWMCVGCYRCTAHCPQDVEFTNILKVLREMATEEGYVD
                     THWIKMVEDIDRRTQKLRRDLIDYLWEEKIPSFQEYVNFYQEETRKLPWVEKQGEGKN
                     D"
     gene            7549..11943
                     /locus_tag="PK016_03585"
     CDS             7549..11943
                     /locus_tag="PK016_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXL02186.1"
                     /translation="MTENKVGKVLVVGGGIAGIQSALDLANSGFKVYLVESSPSIGGL
                     MARLDKTFPTNDCAMCILSPKLVECGRHLNIEVLSYSEVESIEGEAGNFTVGVRRKAR
                     FIEEDKCTGCGDCAEVCPVDLPNEFEGKLSNRKAIFRYFPQAFPGAFTIEKKGMPNCQ
                     AGCPLEQKAEGYIALIKKGKFEEALRTVYMDNPFPGICGRACHHPCMERCQRAILDEP
                     LGIPYLKRFLADYGKEKGLRPLTLPEEKKGERVAIIGAGPSGLSCAYFLSLRGYEVTV
                     LEKEKKAGGMMTFGIPSYRLPREVVGEEIKIIENSGVKIVYGREWGKDFSISTLFEEG
                     FSSIYVAAGAWKGMKLGVEGEDHPRVWDGLRYLVSLNQGNDIPEAENVVVIGGGNVAI
                     DCARSALRQGAKRVSVYYRRSREEMPARAEEIEEAQEEGVEFYFCASPRRFTERDGHL
                     FMETYVMRLCAPDESGRRRPEIIPGAGFEVEGDLFLVATGQKVDLPEGELKLTPSGTI
                     MVDDALRTSREGVFAGGDVVLGPATLVEAIAQGKRAALSIASFLEGKEFREEKRHPAP
                     LNIPWNREREPQVKIKKLEPSERVKDFREVSAGYTGEEAQKEAKRCLACGGCAECGQC
                     VLACQRGAINHQEKDREEKIEVGAIICATGAKVFDPSSAYRELGYKKYPNVVTSLDLE
                     RILNASGPFQGKVVRPGDGKVPRKVAFIQCVGSRDPERGQPYCSSVCCMYAIKEALVA
                     KEHLLLEGEKDFQVAIFYIDLRAYGKDFERYYERAKKEGIRFVRSKIDKVKELDNGDL
                     ELYYSNPQGGVEKENFDLVVLSVGLEVEEKEKDTLENLGFSLSPEGFPYTDSLFPVRS
                     LKEGVWIAGTLGGPKDIPDTVVEASAAASEVASFLHSSRFQEVKEKEYPRERDVESEP
                     LRIGVFICHCGINIAGVVNIEEVAEKVKKLPGVAYVETNLYTCSQDTQERIKEKIKEY
                     HLNRIIVASCSPRTHEVLFQETLREVGLNPYLFEMANIRDQCSWVHQLQPEEATKKAQ
                     DLVSMAVAKASFLEPLQPISLPVDKSVLIVGGGIAGLVAAKEAQSQGLRVYLVEKEKE
                     LGGQLRGFAHRFTLEGKDLVEFKNSLEKDISEHPDIEVFREAEVVEVNGFVGNFESKL
                     RIRGEEKVIRHGGVIIATGATEYHPQEFHYGEDQRIITQVELEKMMERGLSSFPRRVV
                     MIQCVGSRDEKHPYCSKVCCSTALKNALALKKLSPQTEICILYRDMRSYGLAESYYRE
                     AREKGVVFIPYPDDALPSLSLGEDITLHLRSSLGEEVTLLSDLVVLSAGIEPHSNDKV
                     AQLFKVPLNQDGFFLEAHVKLRPVDFATDGVYVCGLAHSPKPARESILQAKACVSRLM
                     NVLSKDTIQSGAQIAEVIKERCTACGDCEKVCQYRAVKVNQEKKVAEVNAALCKGCGL
                     CSATCKSGAIRVQGFTPEEIVSEVEYIL"
     gene            11940..12374
                     /locus_tag="PK016_03590"
     CDS             11940..12374
                     /locus_tag="PK016_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HXL02187.1"
                     /translation="MKNDWEPKIVAFLCHWCSYAGADLAGISRIQYPPNIRVIRVPCS
                     GAVNPLYILKALREGADGVLVSGCHPGDCHYLSGNYFARRKFFVLHELLQWLGVEKDR
                     VQFSWVSASEGQKFSQVVEETVEKVKKLGPAQKLVKEFNFYV"
     gene            12367..13302
                     /locus_tag="PK016_03595"
     CDS             12367..13302
                     /locus_tag="PK016_03595"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXL02188.1"
                     /translation="MFEKEIRRKAKELLEAKAVDLVIGYTSGSLPFQARPILVSSPED
                     TEQLVWNHFCSPNLARYLLPYRESSKKIALLAKGCDARAVVVLLREEQFPRERIHIIG
                     LPCSGIISKRKLEEKLGEIKEEDFSWEENSFFCQGKKWEVDSFLEDTCARCRYPNPPL
                     WDELIGEKRDSRGSGVDYLEKISSQAEEERWQFFTEELSRCIRCYACRNACPLCYCKE
                     CFAESDVPRWISGVPTLSDNLFFHLGRSMHLAGRCVECGACERACPEEIKIGLLPLKV
                     EEIIKNNFSFEAGLSLGEEAPLLTYKENDPGDFIK"
     gene            13311..14291
                     /locus_tag="PK016_03600"
     CDS             13311..14291
                     /locus_tag="PK016_03600"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXL02189.1"
                     /translation="MSEGLIAKNKWWEWIEDKKDRIFLTERAKERYGEWFPPEVKMVK
                     NGKEVLLPPGEEMYRYLWAGEEWKIEEREVSGEPSFLFLLPCEARAIIEVTDKVFLHA
                     PVDKNYWLRRKSLSLGILACLQPEPTCFCSLVGGGPFWRNEEALFLVPRKEDIYIEGR
                     GEPFSFSEEAGEKIKQEVQALQKEAEKGLSSPLPSYFSQELYHHFEDEEWNALSWRCI
                     NCGACTFLCPTCYCFDLNVEGKLKGLQLRSWDSCMFPKFTLHASFHNPRPTGKERVRQ
                     RVMHKFSYFPEREGNFACVGCGVCVEKCPVNWDIREVLERMVEKIDRLRA"
     gene            14272..15114
                     /locus_tag="PK016_03605"
     CDS             14272..15114
                     /locus_tag="PK016_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HXL02190.1"
                     /translation="MTASELEVNNPYLPLLLPIVEIIEETRDIKTFRLQGDSIFSYLP
                     GQFAEVFVPGRGEAPFSITSSPTSRGVLEFSIKRIGTVTTALHRLGEGDKIGVRGPYG
                     NGFPLEELKNHNLLFVGGGIGLAPLRSLINYVLSPQHRGEFGEVFILYGARSPEDLVF
                     KWELEKWQERKDLHLLVTVDRGNNSWKGRVGLVPQVLKEEVKVEVEEWKSIVCGPPIM
                     IKFTIKSLLEMGFSPSQIITTLEMKMKCGLGKCGRCNIGPFYVCQEGPVFSYEVLQNL
                     PEEY"
     gene            15136..16482
                     /locus_tag="PK016_03610"
     CDS             15136..16482
                     /locus_tag="PK016_03610"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HXL02191.1"
                     /translation="MTYEEAVKYLYGLINYEKINFSYSDLKLDRMRELVSRLGNPQND
                     FPSILVAGTKGKGSTAYFLERLFLAWGWKCGFYTKPHLLTFRERIRLGGNLIPASDLA
                     QLVEEIKPVVEEMKNSPWGRPTYFEVSVALAFLYFSRQKVDMAVIEVGLGGRLDATNV
                     CEPQLTVITPISFDHTEILGNTLSAIAREKAGILRKGVKLVLSPQEEEARETIVKEAQ
                     EKGAPVSEVKKECQWQILSRGKDGSLFSFSVKDWGEGVSHLSLLGDHQVTNLLTALLT
                     AREWGLPFDQEKLSKALKDLYWPGRVQVISPEPLVVFDVAHNQASFSQLINTLRNFLG
                     VEKAVFLLGLLEGKDYPGIARELRGFAPYVFLTLPLNPKALLPQVISPYLEEEGIKVE
                     IVKDSFLARDLAFQYAQEQKLPLVVAGSFYLAPLFAHQLLLPPEEEKAYVNSSRKN"
     gene            16457..17257
                     /locus_tag="PK016_03615"
     CDS             16457..17257
                     /locus_tag="PK016_03615"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HXL02192.1"
                     /translation="MLIVAERINATRKPIREALERKDEAFFIQEAKKQEAAGAHFIDV
                     NAGTEASQEIENLPWLVELIQDEVSIPLSLDSANDQALARALQVYNKKEVMINSFTAE
                     EERIKAILPLAKDYNALVVGLAMGKGGIPQTVEDRIKLVDELVEAVDYYKIEKDKLLV
                     DPLVVPIGTDHYQGKVFLETVKAVKEKYNEVRTICGLSNISYGMPNRKLLNRTFLVLA
                     LGYGLDASIMDPLDQEMMSGLYAALALLGYDEFCMNYLSAYRSGKLGS"
     gene            17311..18138
                     /locus_tag="PK016_03620"
     CDS             17311..18138
                     /locus_tag="PK016_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02193.1"
                     /translation="MFKLVLAAILLAILAFPALASEDVILDYALCVGLDQDGMPLERR
                     DIFIVEEEKILCWVFISEVHKGDELRWEFQPSQGEVYESILIVEEDKQYVGATGELPL
                     VTYHNTISPGEWKVTFYLNGEKYLELPFTLLQSDIKESETLEEAIQRTIAVVRKLGYE
                     VLDLNIDDSNQAYLRMNMVASTLDQRVWNQIGIGFESLQRIFPQVSWFLVQLILNDEY
                     VLSFQVRAYDFDMWRKGNLNTDEFWKKKVRRYVYDLKNKEEIQDVSSFYLEKFEVIY"
     gene            18153..18983
                     /locus_tag="PK016_03625"
     CDS             18153..18983
                     /locus_tag="PK016_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02194.1"
                     /translation="MREVFSKFSPRDFQFLADLVGENRDKSYFIDLVTKDPDSLDLVL
                     EEEEIFKKAAQQKENVFVRISPYLYFELLLRQAIRDIKRDTYTLERVGYKTRVPVFDS
                     QKVLEILSRDEIRDYLVELLVSFIRVNQFTVYFRRGKGIYYKKVLSDMDFDLLVEMSE
                     IVDEPYRFVFLKRAGDLALFLSGIFPEQLFGEQSYSLARNKRSIPWANKEEDLEKKGS
                     ELYRKASESEVAKEKELSSILTMMADNFTFTKKPLNVMADRYLYLRKHDIFPFIDNQN
                     "
BASE COUNT         5655 a         3231 c         4872 g         5529 t
ORIGIN      
        1 aattatatag tatagttata gattttccca tcaccttatg ttaagatagc gaaaaattaa
       61 gagagggagg gtccgaaatg gcggaaattt tggaccagat agcaaaggag ctttttgctg
      121 gtaatgccaa agctgtggca gagctaacac aagaagcttt gagtaaaggt ttttctcctc
      181 aagaaatttt gaatgaaggc ttaataaaag gtatgaacga agtagggatg aaatttaaag
      241 ctaatgagat ttatgttcca gaagttttga ttgctgctcg agctatgaag gcggggatgg
      301 agattctaaa acccaaatta gcagaaaccg gagtagaacc agtgggcaag atgattattg
      361 gaacagtaaa aggagacctt catgatattg gtaagaattt ggtgagtatg atgatggagg
      421 gagctggctt tgaggtaatt gatttaggta tcgatgtgcc ggcggagaag tttattcaag
      481 ctgtaaagaa atatcaaccc cagctcgcgg gtatgtctgc tcttttaact accactatga
      541 tacagattag agagaacata aaagcttttc aagaagcagg tatcagagac aaggtaaaga
      601 ttatgattgg aggagctcca gtaactcaaa aatttgctga tgaagtagga gcggatggct
      661 atgctcctga tgctgcttca gctgtagata aagccaagga gctactgggt attagctaac
      721 tgtatattct gatagctaac agggaaaaac attcctttat tgactataaa tgtgattata
      781 gttaaccata aatctatggc gatgtttctc cgcttgaagt ccaatttttc ttcgtgttgt
      841 atgattccct ggtgtcaatt gcgagcgctg aggggaaaag ttcccctcag caatttttta
      901 ttgcttttaa gtttttgtat ttttaatctt acgctatcag gtggctctat tttcttgcat
      961 acgagtgatt ccaaatttta aactcgatgg ggatctcttt ttaattttgg ggattatatc
     1021 aaggtttgtg aaatattata attactttag ttaatgaata atttaaactc aggagggtag
     1081 aaatgttaag cgatttagaa attgcgcaaa gagcgcagat gaaacctatt gttgagatag
     1141 cggaaagtct gggattgtct cctgacgata ttgatctcta cggcaaatac aaagctaaag
     1201 tttctttaga agtattaaaa aacctttctg aaaaacctca gggtaaatac attgacgtta
     1261 cagctattac ccctaccccc ctgggagagg gaaaaacagt aaccaccatt ggtctttccc
     1321 aagctctcaa taaaattggt aaaaaaagta tagtttgcat ccgccagccc tccttgggtc
     1381 cggtttttgg aattaaagga ggagcagcag gaggtggata ttcacaagta gtacctatgg
     1441 aagattttaa tcttcatctt actggtgata ctcatgctgt ggctactgct cacaacttac
     1501 tggctgcttt tattgacaac catctccatc atggcaataa attaaatatt gaccccttta
     1561 ctattagctg gcctcgagtg gtggatatca gcgaccgagc gctgcgcaaa atagtcatcg
     1621 gcttgggagg gaaaaccaat ggagtccctc gagaaagtag ctttgatatt gctgtagctt
     1681 cggaagtaat ggctattttg gctttgacta ctgatatttt tgacttgcgg gcacgattag
     1741 ggcgaatagt tattggttac gataacaata aaaaaccggt taccgctgaa gacttgcact
     1801 gtgccggagc aatgacggtt cttatgaaag aagctattaa acctaatctt ttacaaacct
     1861 tagaaaatac cccttgtttt gttcatgctg gaccttttgc caatattgct catggaaata
     1921 gctctattat tgctgaccag attgctctgc gcttgagcga ttatgtggtt accgaaagcg
     1981 gctttggtgc cgattgtggt atggaaaaat ttatggatat caaatgtcgc tatagcggcc
     2041 taattcccaa ttgtgtggtg atggttactt ccgtcagggc cttgaaaatg cacagtggaa
     2101 aatataaggt agttgctggt aaacctctgg acacggggct cacttgtgaa gatatagagg
     2161 cagtggaaaa aggagcagaa aatatggtga aacagattga aaatgctcgt ctttttggag
     2221 tgccggtggt agtggctatc aatctgtttg cttctgacac tgatagagaa gtggaaacgg
     2281 tgggaaaaat cgctctggaa aacggagctt ttgctgtgga ggtatcagag gtatgggaaa
     2341 aaggtggaga aggaggaaaa gatttagcgg aagcggtggt aagagcctgt aatgaacctc
     2401 aaaatttcca cttcctctat cccttagata ttcccattaa agagaaaatc gaaattatag
     2461 ctacccagat ttatggtgcc gatggagtaa cgtatgaacc caaagccgaa gcagggatta
     2521 aaagatttac cgagttaggt tgggataaac tccctatctg tatggccaaa actcatcttt
     2581 ctctctctca tgaccccaat ttgaaaggta ggcctcgagg atttaaagtt cctgtccgag
     2641 acatccgccc ttctatcggt gctggatttc tctatcctct ttgtggagag atgagcacta
     2701 tgcctggctt gccctctgaa ccggcaggga ataaagtaga tattgacgga gaagggaaag
     2761 tagtaggtct tttttaaggg agggaaaagt gaactacgac ttggtaataa tagggggagg
     2821 agtagcagga ttaactctcg ctcaaacctt gcaggataag ctggaagttt tagtggtaga
     2881 agagtcatct ctggggggga tggcctcaaa gttaggatgt aaagctactg atgagtgcct
     2941 ctcctgcgga gtttgccgat ttatcaatgt agaaagagag ctttgtttta aaaaatttga
     3001 tatttgggaa ggtagaaaga taacttctgt agataaatcc ggcaggggat ttcacctaaa
     3061 aactgaaaaa ggagaaaaag ttaccagcca gctgctggct gtggcaactg gtgcctctcc
     3121 tttagagatc aaaaatcttt ttcacctggg agggggctta ccccgggttt atagtggctg
     3181 ggatgtagaa agaggtctaa atgaaggtat tatttcctct tttcgagatt tttcctcttt
     3241 agcttttatc cagtgtgctg gctctcgcaa tttcaaagaa aagcggggtt attgctctca
     3301 agtttgttgc cgctatgctt tgcgtatcgc tgaaaatctc aaattttctt tcccttctat
     3361 ggacattgat ttttattata tggatttaca aattttagga aagaaaaaag agaaattatg
     3421 ggagatagct ggtaaaatta atctaattcg ccacctacct tatgaagtaa aaaaagaagg
     3481 gggaaagttg tctctggttt ttgaagaaca gggagtggag aggaaaaatt acgatgccat
     3541 tgtactttcg gtgggaatgg ttccttcctc aggaactaaa gaaataggaa agtggttagg
     3601 gcttaatttt gattctgccg gttttataaa atcttatggt ttgggtcata cctcccgtga
     3661 gggagttttt gtatgtggaa cagcttgcgg tcctaaggat attgaaacta ctcaggaaga
     3721 ggctcaaaag gtgggagagg aaatctggcg agtgaggaga ggatgaagtg aataaacttc
     3781 tctggttagc aaatgaggct ttttcttttc ccgatttctg gatagaagag gtggagagaa
     3841 ataacgactg ggaaatagag gtaaaaaagt ggggcgataa actcggtagt tttaccgaat
     3901 acgataggat ttttatttta ggagatagct tttcctttac gcaagaaaaa atatccctcc
     3961 tctctgagga gtgggaagtg aatcctctaa aggttcgagt tttccccggg gaagaagtga
     4021 aaattatttt tcctgagtct tttttatcta tcttggagca gtttataatt caatctctct
     4081 cccaacccct tttccccgtt gaagcccgga gagtggttcc cgaaaataga gttttagttt
     4141 tacctcaatt atctccgact ttagaacaaa ccttgaaaaa aagagggata gatataatag
     4201 ttcctccttc ttctttttat cttcggagag aagggataaa ttttcgcctt tatccttccc
     4261 gagataacgt tggagcgata gtagtctcac ctcttgaaga aaagaaattt ccggcttctt
     4321 tcccttgtga ggtgcaggat actcgaaggt tagtagacat agagtttatg gaaaaagaga
     4381 tagaaaaaaa ggatttcagg aaagaaacta tagcgtttat cattcctaaa aaaggagtag
     4441 ctgaagaaga atggcggaag gcttttcgcc tttctcaaat tctagtagaa agagataaag
     4501 cagaagtttt tgttttagta gaagaggtgt tggtagctgg agaaaacttg gaaaaagaat
     4561 atcggaaggc tcgctcctcc ggggttattt ttgaaaaagt ggatttttcc aagttgggaa
     4621 cgaggcctac tctggatatg cgagggatag aggtagagtt cactactgag cgagataaat
     4681 tgaggcggga agtaaaagcg gattggttgg ttctgcttcc cgagaggaat atccttcctt
     4741 ttgatctcgc tcctttcttt tgcggagata aaataggggg agaaattttg cctctggaaa
     4801 atcccaatgt tttgcctttt tcttcgggaa tagaaggaat ttttctttct ccccccggag
     4861 aggaagagga gctttctctg gttataaaaa aatatttaac cgagggagta gtaagggaga
     4921 taggtagagg agaggtagac gaagaaaaat gtgttctgtg tcttacctgt ttgcgcacct
     4981 gtccctggga ggctatagaa atagaagggg aaaggaaaaa agctcgaata aatgaggaac
     5041 gatgccatct ctgtgggctc tgcgcttctt tttgcccggc tcacgctatc gaaataaaag
     5101 gtttatcacc tgaaaactgg gtctctgcag tctctttagg aggaaaaaat gagagtagta
     5161 gctttaggtt gtgataaaag tggttatcct gctcttcttc ggtggggaga agaagattct
     5221 cttccttttg atgaagtaaa aattttttct ctcccttgtc tgggtttggt gaccgaggag
     5281 gtaattttcc gcattttgga aaatagtgac gctctgcttt tggctggttg ccctctggat
     5341 agctgccaca atcaaaaggg aagccaggga gcctatctca aagtacaaag ggtgaattct
     5401 ctattacaag aagccgatat ctcgaaaaaa gtagctatag cttttgtcac ttccggtaaa
     5461 gtaggggaga tagaaaaagt gagtgaggag ctcctctgga gagaaaaatg ataatagctg
     5521 agcgaaagcc gattaaagaa attttgtctt ttttagggcc cggagaaaaa atcctggttt
     5581 taggttgtgg gacttgtgtc acagtgtgta tgtcgggagg agaaaaagaa tcccgggagc
     5641 tctcttctct tttgcgcttg aaaggatggg aagcaaaaga tttaaccgta gagaggcaat
     5701 gtgagccaga atttaatcta cgggtgagag aagaaatagc gaaagtagat acaatgattt
     5761 ctttagcctg tggagtggga gtacaggctc tcgccgagtg ctttccgggg aaaaagattt
     5821 tccccggcct caacactacc tttatgggta tgccgaaaga gcagggctta tgggaggagc
     5881 gatgctgggg ttgtgggaac tgcatcgtac atctttttgg ttatatctgt ccgattactc
     5941 gctgtgctaa aagtctcctg aatggaccct gtggaggttc aaaagaggga aaatgtgaag
     6001 taaagcccga tagagattgt gcttggcagc tcatctatga ccggctaaaa agagaaaatc
     6061 gattggaggt tctgtatgcc cttcagcctc cccgggattg gtctacttct cgctatgggg
     6121 gacagaggaa aatgatacgg gaggatgtcc ggttatgagc tctttagaag aaattcttcg
     6181 ccggggagag tttgcagtta cggcagaagt gggacctccc aaaggaacca accgagaagt
     6241 aatagagaaa aaatgtgctg aactgcaggg ttttgtggat gcggtaaata tcaccgataa
     6301 tcagactgcc attgttcgtc tttccagctt tgccagctgt ttgatagcta aagataaagg
     6361 gatagaaccg gtgatgcaga tggtctgcag agaccgaaat cgtatcgctc tccagagtga
     6421 ttttttaggt gcttgtgctt ggggagtgga gaattttctc tgccttactg gagaccacca
     6481 aagtatgggg aatcaccctc aatctaagaa tgtttttgat gtagattcta tacagctttt
     6541 gcaaattttc aaaaatatga gggatgaaaa agtttttcaa aatggtgaac ccttaaaagg
     6601 agagataaaa gcttttctgg gagcaggaga aaaccctttt gccgaccctc tggaatttcg
     6661 ggtaattcgc ttagctaaaa aaatatcagc gggagcacag ttcatccaga ctcaggcagt
     6721 ttttgacctg gaaattttct cccgatttat ggaagaggta aggaaaatgg gtctggataa
     6781 aaaagccttt attttagccg gggtgattcc agttaagtct ctggcggctt tgaattatat
     6841 gaaaaacgaa gttccggggt tagtggtccc tgattattta gtagagagaa tgagggaggc
     6901 taaagacccc aaagaggagg gagtaaaaat ctgcttggaa actattgagc agctgaaaaa
     6961 gatagaagga gtgaggggag tgcatattat ggctatatcc tgggaaagca tagtcccaga
     7021 tatagtaaag ggagctaact tgtaccctcg ttcggggagg agctaaagtg atggctgtgg
     7081 attatcaatt caaatatcag gtggcgaatc gtctaggggg agaaggatta ttggtttgtt
     7141 tttcctgtgg tatctgtact gccgggtgtc cggtggcaga ggtagaagga ggttttaacc
     7201 ctcgacgaat aattcatgaa gtgttattgg gagaaagaga aaaagtgtta cgctccaagt
     7261 ttatctggat gtgcgtgggg tgttaccgtt gcactgctca ttgtcctcaa gatgtagagt
     7321 tcacaaacat tttgaaagtt ttacgagaga tggccaccga agaaggttat gtagataccc
     7381 actggataaa aatggtagaa gacatagacc gccgaaccca gaagttacgt cgggatttga
     7441 ttgattatct gtgggaggaa aaaatcccct cttttcagga atatgtcaat ttttaccagg
     7501 aagaaactcg gaagctcccc tgggtggaaa aacaaggaga aggaaagaat gactgaaaat
     7561 aaagtgggta aagttttagt ggtaggagga ggaatagcgg gcattcagtc ggcactggac
     7621 ctggctaact cgggctttaa ggtatatctg gtagaatctt ctccttctat tggggggctg
     7681 atggctcgtc tggataaaac tttccccact aatgattgtg ctatgtgtat tctctctcct
     7741 aaattagtgg aatgtggaag gcatctcaat atcgaagttc tctcctactc agaagtagag
     7801 tctattgagg gagaagcagg taatttcacc gtaggggtga gaagaaaagc ccgatttata
     7861 gaagaagata agtgcactgg ttgtggtgat tgtgcagaag tttgtccggt ggatttacct
     7921 aacgagtttg aaggaaagtt gagtaacaga aaagccatat ttcgctattt tcctcaggct
     7981 tttcctggtg cttttactat tgaaaaaaaa ggtatgccta actgccaggc aggctgccct
     8041 ctggagcaaa aagcagaggg ctacatcgct cttataaaaa aaggaaagtt tgaagaagcc
     8101 ttgcggactg tctatatgga taatcctttt cccggtatct gtgggagagc ttgtcatcac
     8161 ccctgtatgg aacgctgcca gcgagctatt ttagatgagc ctctgggaat accttacctc
     8221 aagcgatttt tagctgatta cggaaaagaa aaaggtcttc gtccacttac cttgcccgaa
     8281 gaaaaaaagg gagaaagagt agccataatt ggagcaggac ccagtggttt gagctgtgcc
     8341 tattttttaa gtttaagagg ctatgaagta accgttttag aaaaggaaaa gaaagctgga
     8401 ggaatgatga cctttggtat tccttcttat cgtcttcctc gagaggtagt gggagaagag
     8461 ataaaaatta tcgaaaactc gggagtaaaa atagtctatg gccgggaatg gggtaaagat
     8521 ttctctattt ctaccttatt tgaggaaggt ttctcttcta tatatgtagc tgcgggagcc
     8581 tggaagggga tgaagttagg ggtagaagga gaagatcacc ctcgagtatg ggatggcctg
     8641 cggtatctgg tttctctaaa ccagggaaat gatatccctg aggctgaaaa tgtagtggta
     8701 attggaggag gtaacgtcgc tatagattgt gctcgcagtg ctctgcgcca gggagcaaag
     8761 cgggtttcgg tgtattatcg ccgttctcga gaagaaatgc cggctcgggc tgaggaaatc
     8821 gaagaagcgc aagaggaagg ggtagagttt tatttttgtg ccagccctcg gagattcact
     8881 gagcgagatg gccatctatt tatggaaact tatgttatga gattgtgtgc tcctgatgag
     8941 tcgggaagaa ggagaccaga aattatccct ggagctggtt ttgaagtgga aggagacctt
     9001 ttcttggtgg ctacgggaca aaaagtagac ttaccagaag gggagctaaa attaactcct
     9061 tcggggacaa taatggtaga tgatgctttg cgtacttctc gagaaggagt ttttgctggg
     9121 ggagatgtgg ttttaggtcc ggctactttg gtagaagcta tagctcaggg aaaaagagcc
     9181 gctctttcta ttgccagttt cttagaaggg aaagaattcc gagaagaaaa aagacatcct
     9241 gccccgttga acattccctg gaatagagaa agagagcctc aagtaaaaat aaaaaagctc
     9301 gaaccctcag agagagttaa agattttaga gaggtgagtg caggatacac cggcgaggag
     9361 gcacaaaaag aagcgaaaag gtgccttgct tgtgggggat gtgcagagtg cgggcagtgt
     9421 gttttagcct gtcagagggg agctataaac caccaagaaa aagatagaga agaaaaaata
     9481 gaggtaggag caataatttg tgctaccgga gcgaaagtat ttgacccctc ttcagcttat
     9541 cgagaactgg ggtataagaa atatcctaat gtggttacca gccttgattt ggagcgcatt
     9601 ctcaatgctt ctggtccttt ccaggggaaa gtggtaagac ccggagatgg gaaagttccc
     9661 cgaaaagtgg cttttatcca gtgtgtgggc tcccgagacc cggaaagagg ccaaccttat
     9721 tgctcttcgg tgtgctgtat gtatgctatt aaagaagcgc tggtagctaa agaacattta
     9781 ttgttagaag gagagaaaga tttccaggtt gctatttttt atattgacct tcgcgcttat
     9841 gggaaagatt ttgaacgcta ttatgaacga gctaaaaaag agggaatccg ttttgtccgg
     9901 agcaaaatag ataaagtgaa agaactggat aatggagatt tggaacttta ttattctaat
     9961 ccccagggag gggtagaaaa agaaaatttt gatttagtag tgctgtcagt agggttggaa
    10021 gtggaagaga aagaaaaaga tactctggaa aatttgggtt tctctctcag cccggagggc
    10081 tttccctata ccgattctct ttttcctgtt cgttccttaa aagaaggagt atggatagca
    10141 ggaacattgg gtggacctaa ggatatcccc gatacggtag ttgaagccag tgctgctgca
    10201 agtgaggtgg cttcttttct tcattcttct cgcttccagg aagtaaaaga aaaggaatat
    10261 ccccgggaga gggatgtgga atctgaacca ttacgcattg gagtgtttat ttgccactgc
    10321 ggtataaaca tcgctggagt ggtaaacata gaagaagtag cggaaaaagt aaaaaagctt
    10381 cctggggtag cttatgtaga gactaacctt tatacctgtt ctcaagatac tcaggagagg
    10441 atcaaagaaa aaataaagga atatcatcta aatagaataa tagtggcttc ttgttctccc
    10501 cgcactcatg aggttctctt tcaggaaact ctccgggaag tggggctaaa cccttatctt
    10561 tttgaaatgg ctaacattcg cgaccagtgt tcctgggtgc accaacttca accagaggaa
    10621 gccaccaaaa aagctcagga tttagtatct atggcagtag ccaaagcctc ttttttggag
    10681 ccgctacaac ctatttccct tccggtagat aaatcagttc tcatcgtggg aggaggaatt
    10741 gccggtttag tggcagccaa agaagcccaa agtcaaggcc tgcgggtgta tcttgtggaa
    10801 aaagaaaaag aattgggagg gcaactgcga ggttttgctc accgctttac tttagaaggg
    10861 aaagatttgg tagagtttaa aaatagtttg gagaaagata tcagcgaaca cccagatata
    10921 gaagtgtttc gagaggcaga agtagtagaa gtcaacggtt ttgtgggtaa ttttgaaagc
    10981 aaactccgta tcaggggaga agaaaaagtt atccgccatg gaggagtgat tatcgctacc
    11041 ggcgctacag aatatcatcc tcaagagttc cactatggag aggaccagag aattattacc
    11101 caggtagagt tagaaaaaat gatggagaga ggattatctt cttttccccg gcgagtggtt
    11161 atgatccaat gcgtgggctc aagagacgag aaacatccct attgcagtaa agtctgttgc
    11221 tctacagcgt taaaaaatgc tctagctctc aaaaaactct ctcctcaaac cgagatatgc
    11281 atcctttatc gagatatgag aagttatggg ttagcagaaa gctattatcg ggaagctcga
    11341 gaaaaagggg tagtttttat cccttatcct gatgatgccc ttccttctct atctttaggg
    11401 gaggatatta ctttgcatct ccggagttcc ttaggagaag aggttaccct tcttagtgat
    11461 ttggtggttt tgagtgccgg gatagagcct catagtaacg ataaagtggc tcagctattt
    11521 aaagtgcctc tgaatcaaga tggtttcttt ttggaagctc atgtaaagct aaggccggtg
    11581 gattttgcca ccgatggggt ttatgtttgt ggtttagctc acagtcctaa accagcccgg
    11641 gaaagtatcc tacaagctaa agcctgtgtt tctcgactta tgaatgtttt aagtaaagat
    11701 actattcaat ccggggcaca aattgctgaa gtgataaaag aaagatgcac cgcctgtggt
    11761 gattgtgaga aagtttgtca gtatcgagcg gtcaaggtaa accaggagaa aaaggtagct
    11821 gaggttaatg ctgctttgtg caaagggtgt ggtctttgta gtgctacctg taaaagtgga
    11881 gctattcggg tgcaaggctt taccccggaa gaaatagtct ctgaggtgga atatatatta
    11941 tgaaaaatga ttgggagcct aaaattgtag cttttctgtg ccactggtgt agctatgccg
    12001 gtgctgattt agcaggaata agcaggattc aatatcctcc taacatccgg gtgattcgag
    12061 ttccttgttc gggagcagtt aatcctcttt atattttgaa agctctgcga gaaggagcag
    12121 atggggtttt ggtttctggt tgtcatcctg gtgattgtca ctacttaagc ggtaattatt
    12181 ttgcccgacg caaatttttt gttcttcatg aattgctaca gtggctggga gtagaaaaag
    12241 atagggtgca attttcctgg gtttcagctt cggaagggca aaaattttct caagtggtag
    12301 aagagacagt agagaaagtg aaaaaattag gtcctgctca aaagttggtg aaggagttta
    12361 atttctatgt ttgagaaaga gatacgaaga aaagctaaag agcttttaga agcaaaggca
    12421 gtagacttag ttattggcta tacctccggg tctctacctt ttcaggccag acccattttg
    12481 gtttcttctc ctgaagatac agagcaactg gtgtggaatc acttttgcag ccccaattta
    12541 gctcgctatc ttttacccta ccgagagagc tctaaaaaaa ttgctctttt ggctaaaggg
    12601 tgtgatgctc gggcggtggt agttctcctc cgggaagagc aatttccccg agagagaatc
    12661 cacataatag gattaccgtg ctcggggata atcagtaaaa gaaaattaga agagaagctg
    12721 ggagagataa aggaagaaga tttctcctgg gaagaaaata gttttttctg ccagggtaaa
    12781 aaatgggaag tagactcttt tttggaagat acctgtgccc gttgccgata tcccaacccc
    12841 cctctttggg atgagctgat aggagagaaa agagactcgc ggggatccgg cgtagattat
    12901 ttagaaaaaa taagtagcca agccgaagaa gagagatggc agttttttac cgaagaattg
    12961 agtcgctgta ttcgctgtta tgcctgtcgc aatgcctgtc ctttgtgcta ttgtaaggaa
    13021 tgttttgccg aaagtgatgt gcctcgctgg ataagtggtg ttcccactct ctccgataac
    13081 ttatttttcc atttaggtag aagcatgcat ttagctggaa ggtgtgtaga gtgtggggct
    13141 tgcgaacggg cttgtccgga ggagataaaa attgggcttt tgccgctaaa agtagaagag
    13201 ataatcaaaa ataatttttc ttttgaagcc gggttgtcac ttggggaaga agctcctctt
    13261 ttaacttata aagagaatga ccccggggat tttataaagt gagggaagcg atgagtgagg
    13321 gattaatagc taaaaacaaa tggtgggagt ggatagaaga taaaaaagac agaatatttt
    13381 tgaccgagcg ggctaaagag agatatggag aatggtttcc cccggaggta aaaatggtta
    13441 aaaatggaaa ggaagtgctt cttcccccgg gagaagaaat gtatcgttac ttatgggcgg
    13501 gagaggaatg gaaaatagaa gaaagagaag tttctggtga gccttctttt ctttttctcc
    13561 ttccctgtga ggctcgggca ataattgaag taaccgataa agttttttta catgccccgg
    13621 tagataaaaa ttattggttg agaaggaagt ctctttcttt gggaatttta gcttgtctcc
    13681 agccggagcc cacttgtttt tgctctttag tgggaggagg tcctttttgg agaaatgagg
    13741 aagctttatt tttggttcct cgaaaagaag atatttatat cgaagggaga ggagagcctt
    13801 tctcctttag tgaagaggca ggagaaaaaa taaaacaaga agttcaagcc ctacaaaaag
    13861 aagctgaaaa ggggctttct tctcctctac cttcttattt ttctcaggaa ctttaccatc
    13921 attttgaaga tgaagaatgg aatgctctca gctggcgttg tataaattgt ggcgcctgca
    13981 cttttttgtg tcctacctgt tattgctttg acttgaatgt agagggaaaa ttaaaagggc
    14041 ttcagctccg cagctgggat agctgtatgt ttcctaaatt tactctgcat gcttcttttc
    14101 ataaccctcg tcctacagga aaagaaagag tgaggcaaag agtgatgcac aagttttctt
    14161 atttcccgga gcgagaggga aattttgcct gcgtgggttg tggagtatgc gtagaaaaat
    14221 gcccggttaa ctgggatatt agagaggttt tagagaggat ggtggaaaaa attgaccgcc
    14281 tcagagcttg aagttaataa tccttatcta cctcttcttt tacctatagt agaaattatt
    14341 gaggaaacca gagatattaa gacttttcgc ttacaaggag actctatctt ttcctatctt
    14401 cccgggcagt ttgctgaagt gtttgttccc gggagaggag aagccccttt ttctataact
    14461 tcttctccca ccagtagggg agttttagag ttcagtatta aaagaatagg aacagtaacg
    14521 acggctctgc atcgtttagg agaaggagat aaaatagggg tacgaggtcc ctatggaaat
    14581 ggttttcctt tagaagagct aaaaaaccac aatctccttt ttgtgggagg agggattggg
    14641 ttagctcctt tgcgttctct cattaactat gttctttctc ctcaacaccg aggagagttt
    14701 ggagaagtgt tcatccttta tggagcgagg agtccggaag atttagtgtt taagtgggag
    14761 ttagaaaaat ggcaggaaag aaaagacctc catctactcg ttactgtaga ccggggaaat
    14821 aactcctgga agggaagagt ggggttagtt cctcaagttc taaaagaaga ggtaaaagta
    14881 gaggtggagg agtggaaaag tatagtttgt ggcccaccga ttatgataaa atttaccatc
    14941 aaatctcttt tagaaatggg tttttctcct tcccagatca ttaccacttt ggagatgaag
    15001 atgaagtgcg gtttagggaa atgtggtcga tgtaatatcg gacctttcta tgtttgccag
    15061 gaaggaccgg ttttttctta tgaggtgttg cagaatttac cggaagaata ttgaaaggga
    15121 aaagagggag cggcattgac ttatgaagag gcagtaaagt atctctatgg tttgataaat
    15181 tatgaaaaaa ttaacttttc ttattctgat ttaaaattag atagaatgag agaattggta
    15241 agcaggttag gtaatcctca gaatgatttt ccctctattt tagtagccgg gacgaaaggg
    15301 aaaggtagca ctgcttattt tttagaacgc ctctttttag cctggggatg gaagtgtggt
    15361 ttttatacca aacctcacct tttgactttt agagaaagaa tccgcttagg tggtaatcta
    15421 attccggctt cggacttagc gcagctagta gaagaaataa aaccggtggt agaggaaatg
    15481 aagaattctc cctgggggag gcccacttat tttgaagtat cggtagcttt ggctttcctt
    15541 tatttttccc ggcaaaaagt agatatggcg gtaatagaag tggggttagg aggaaggtta
    15601 gatgctacca atgtttgtga gcctcaactt accgtcatca cccctatcag ctttgaccat
    15661 acggaaatct tgggaaatac cctttctgcc attgcccgag aaaaagcggg gattttgaga
    15721 aaaggagtaa agttagttct ttcccctcag gaggaagaag ctcgagaaac cattgtaaaa
    15781 gaagcacaag aaaaaggcgc gccggttagc gaagtaaaaa aagagtgtca gtggcaaatt
    15841 ttatctcgag gaaaagacgg ttctcttttt tccttttcgg taaaagactg gggagaggga
    15901 gtctcccatc tttctctttt aggagaccat caggtaacta atcttcttac tgccctttta
    15961 actgctcggg agtggggatt gccttttgac caggagaagc tgagcaaagc actgaaggat
    16021 ctctactggc caggtagggt tcaagttatc tctcctgagc ctttagtggt atttgatgta
    16081 gctcacaatc aagcctcttt ttctcaatta attaacactc tccggaattt tttgggagta
    16141 gaaaaggcgg tatttttact cggtctttta gaagggaagg actatccagg tatagctcgg
    16201 gaattaagag gttttgctcc ttatgtcttt ttgactttac ccctaaatcc caaagctctc
    16261 cttcctcagg tgatatcacc ttacttagaa gaagagggca taaaagtgga aatagttaaa
    16321 gactcttttt tagctcgtga cctggctttt cagtatgctc aagagcagaa gttaccttta
    16381 gtggtggctg gttcttttta cctcgctcca ctttttgctc accaattact tttaccccca
    16441 gaggaggaaa aagcttatgt taatagtagc agaaagaatt aacgccactc ggaaacctat
    16501 tcgagaagct ttagagagga aagatgaggc tttttttatt caggaagcta aaaagcagga
    16561 agcagcggga gctcatttta tagatgtaaa tgccggaact gaagccagcc aggaaataga
    16621 aaacctgccc tggctcgtgg aattaatcca ggatgaggta agtatccctc tttctctgga
    16681 ttcggctaat gaccaggcgt tagctcgagc tctccaggtt tataataaaa aagaggtgat
    16741 gattaattct ttcactgctg aagaggaaag aataaaagca attttacctt tagccaaaga
    16801 ttataacgct ttggtagtag gtttggctat ggggaaggga ggaattcccc aaactgtaga
    16861 agaccgcata aaattggtag atgagctggt agaagcagtg gattattata aaattgaaaa
    16921 ggataaactt ctggtagacc ctttagtagt gcctattggc actgaccact atcaagggaa
    16981 agtttttctg gagacagtga aggcggtgaa agaaaaatat aatgaggttc gaactatttg
    17041 cggtctcagt aacatctctt atggcatgcc caatcgaaag ttattgaacc gcacctttct
    17101 ggttttagct ctgggctatg gtttggatgc ttctatcatg gaccctttag accaggagat
    17161 gatgagtggc ttgtatgcgg ctttagccct tttgggttat gatgaatttt gtatgaacta
    17221 tttatcggct tatcgatcag gtaaattagg aagttaaact ggtattaaaa tttattctcc
    17281 ttttaataaa aaagctggag gtatggtatt ttgtttaaat tagttttggc tgctattttg
    17341 ttggctattt tagcttttcc tgccttggct agtgaagacg taattctgga ttatgctcta
    17401 tgtgtgggat tggatcagga tggaatgcct cttgagcgcc gagatatatt tattgttgaa
    17461 gaagaaaaaa tactttgctg ggtatttatt agtgaagtcc acaagggaga tgagttacgc
    17521 tgggagtttc aaccctcaca gggagaagta tatgaaagca ttttaatagt ggaagaggat
    17581 aagcaatatg taggtgctac tggggagtta ccccttgtta cttatcataa tactatctct
    17641 cctggggaat ggaaagtaac tttttatctc aatggagaga agtatctgga acttcctttt
    17701 actctcttac aaagtgatat taaagaaagc gaaactttag aggaagctat tcagagaacc
    17761 attgccgttg ttagaaaact gggatatgaa gttctggatt taaatattga tgatagcaat
    17821 caagcttatt taagaatgaa tatggtggct tctacccttg accagcgagt gtggaaccag
    17881 attggtatag ggtttgaatc cttacaaagg attttccccc aggtatcctg gttcctggtt
    17941 cagcttatat taaatgacga gtatgtgttg agttttcaag taagagctta tgattttgat
    18001 atgtggagaa aaggtaacct taatactgat gagttctgga agaaaaaggt gcgtcgttac
    18061 gtttatgatt taaagaataa ggaagagatt caggatgtgt cttcctttta tctggagaag
    18121 tttgaggtaa tatattgaga aggtgagttt caatgaggga ggtatttagc aaattttcac
    18181 cgagagactt tcaatttctg gctgatttag taggagaaaa tagggataaa tcctatttta
    18241 tcgatctggt aactaaagac cctgattctt tagacctggt actggaagaa gaggaaattt
    18301 ttaagaaagc agcccaacag aaggaaaatg tttttgtccg tatcagccct tatctctatt
    18361 ttgagcttct tctccgtcag gctatccggg acataaaaag agatacctat acactggagc
    18421 gagtaggtta caaaactcga gtgccggttt tcgacagcca aaaagtttta gagattctct
    18481 cccgggatga aatcagggac tatctggtag agctattagt ttcttttatc cgggttaatc
    18541 aatttactgt ttactttagg cgaggcaaag gcatttatta taaaaaagtt ttgtccgata
    18601 tggattttga ccttctggta gaaatgagcg aaatagtaga tgaaccttat cgctttgttt
    18661 tcctgaaacg agcaggagat ctggctttat tcttaagtgg tatattcccc gaacaacttt
    18721 tcggggagca atcttattct ttagccagaa ataaacgatc aattccctgg gctaataaag
    18781 aggaagacct ggagaaaaaa ggaagcgagt tgtatcgcaa agccagtgaa agtgaagtag
    18841 ctaaggaaaa agaactgagc tccatactga ctatgatggc tgataatttt actttcacta
    18901 aaaaacctct taatgttatg gcggatcgat atttatacct cagaaaacac gacatctttc
    18961 cctttattga taaccagaac tgattcttta aataaggttt gtttcttatt ttgcaatata
    19021 gttagccaca aaatttatga tgatatttct catttataga gagggtctct tttctaaggg
    19081 ttatagaggg cgaaaaggtt ctagctgaag taatactctg aaggggcttc aaccaccgtt
    19141 tataatatac aacattgtta tttctgtctg cttccgtgat gaaagaagat tatgcctaac
    19201 tttgttgcaa caccgaaaga gcacagggtc gttgtttaac agagttataa tcaaaagttg
    19261 tgtatggcca aatgaaaaca ggtggcg
//
LOCUS       DAOSVE010000026        17814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_1994_length_17814_cov_84.857424, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000026 DAOSVE010000000
VERSION     DAOSVE010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 17814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17814
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1994_length_17814_cov_84.857424"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..505)
                     /locus_tag="PK016_03630"
     CDS             complement(74..505)
                     /locus_tag="PK016_03630"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HXL02195.1"
                     /translation="MKDIVQQSFRYLNRKMYTRDELSRKLTRDGFLKDEIEKTVQFLE
                     ERGYLDDEEFVKVYIEARRKLNPRGLQTMKDELRRRGISRELLESLREFFPREEEVGD
                     ARGLIKEWLGEGKEKEDINNRLLRRGFSWEVIEEAWYREES"
     gene            complement(502..1533)
                     /gene="recA"
                     /locus_tag="PK016_03635"
     CDS             complement(502..1533)
                     /gene="recA"
                     /locus_tag="PK016_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HXL02196.1"
                     /translation="MSEEKKSRDLREDVINQAISSIERKYGKGAIMYLSQDVLTADVE
                     VIKTGSVLLNIALGVGGFPRGRVVEIFGPEASGKTTIALHAIAEAQKQGGIAAFVDTE
                     HALDPSYASRIGIQIDKLLISQPGSAEEALDIVDALVRTGGVDIVVLDSVAALVPQME
                     LEGGMSDSYIGLQARLMSQALRRLTAYISKTKTVAIFVNQLREKIGMTYGPSETTPGG
                     RALKFYASVRLDVRRRDYLRSGEEIIGALTRVKVVKNKVASPFKEAELELIYGEGISR
                     EGEILNLGDTTGVIKKSGSWFSFGEYRLGQGRDNARQFLKENPEIAKEILSAALSQMG
                     VNPSVAFNS"
     gene            complement(1526..2101)
                     /gene="thpR"
                     /locus_tag="PK016_03640"
     CDS             complement(1526..2101)
                     /gene="thpR"
                     /locus_tag="PK016_03640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HXL02197.1"
                     /translation="MSSVTIRSFVALDFLPEAQDFLEELINQEKKLYPQAKWVKREQL
                     HLTLHFFSAFPSGGVEEMNRILKEVSSKFTAFPVTLERWGAFPSLHRARVIWIGVDQE
                     GEEKIKNIREEVEEEIVSRGWEREDREFSPHITLARFRSPFTLPSLEVENIRFSTEIK
                     KLVFYQSTLTPRGSVYQELGAFSLEEVKKNE"
     gene            complement(2082..3347)
                     /locus_tag="PK016_03645"
     CDS             complement(2082..3347)
                     /locus_tag="PK016_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HXL02198.1"
                     /translation="MKAAILGIGTELLTGLVRDYNAYFLAQKLVDKGVLPQFILFVPD
                     QIEKIVNALQFLMREEEVKLVIVSGGLGPTEDDLTREAVAQTFGRGLVFSSEAWDRIQ
                     FFYRELRNTEPPENNKRQAFIPQGAEILYNERGTAPGFKLEVDGKSIFVIPGVPREAE
                     FFWSLIEKDLPQEEEYFYRSPIFKFCGIGESNLAKEIEPFLSHLPSSFNLAFLPNYGE
                     VWLYVYSSKFSPEEKESALLFLDEVAHHLDKFFFSPYGNTLEEAVGNVMKEKGLKLGL
                     AESCTGGLVANRITDIAGSSQYFERGYVVYSNEAKKEDLSVPEEILKEKGAVSEETAQ
                     FLARGVRQRTKVDLGLGITGIAGPGGGSKEKPVGLTYIALSSEEGVRVQKFHFSGDRL
                     MNKRFASQSALVMVFQYLKEKFGHEFSYH"
     gene            complement(3344..4675)
                     /gene="rimO"
                     /locus_tag="PK016_03650"
     CDS             complement(3344..4675)
                     /gene="rimO"
                     /locus_tag="PK016_03650"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HXL02199.1"
                     /translation="MKKIAFVSLGCDKNLVDAEVALGTLLSRGYNFFSSPEEAQVLIL
                     NTCAFIEEACRETEAWIEKLSFLKEAYPPKKLLVMGCYAQRWGREKSFVYPAVDYWVG
                     VNDFPSLVDVLEDKFPGKCFLDSPPFVYNHETERVLSTPSHYAYLKIAEGCNHRCSFC
                     LIPSIRGSLRSRTMESIEKEALNLVAQGVKEIILVAQDLSSYGLDLYHRKMLSPLLRR
                     LDEILPPGIWLRLLYFSPEGLDCGFIKAMRDSSHIVKYLDIPLQHVDSALLQRMRRPS
                     SIEEIREKLEKLRESVPDIFLRTTFLVGFPGEDEEAFQRLLSFVEDFRFERMGAFTYS
                     ESPDVSSASLYPKVEEKVKQERYKELMKAQEKVMREFHSSLVGRELEVIVDEVQRENG
                     INHCWGRTYGDAPEVDGKISFSSRKSKLKRGEIVKVKVTESSSYELKGERI"
     gene            complement(4672..5424)
                     /locus_tag="PK016_03655"
     CDS             complement(4672..5424)
                     /locus_tag="PK016_03655"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXL02200.1"
                     /translation="MVEGQLKLGEILREKREEKKLTYDEVSGATHISSRYIAALEEEN
                     WDKLPGRAYALGYLKIYSRFLGLNEEEMVSLFNQVYSVKPVDWKSLDWKKTEEPGLRE
                     AKDRRNSRSTLFKRLLAVLISLAVFFAFLFIFFSFNHLPEEGEVVVVNPPVSAIEPEE
                     IFPSPSAEVGPQFSLTVTLLPEKVAWAEIWSGGNEVFSGILVPNKEYVIKSNYPIEVE
                     AKGGNGVKATVNGEDRGYLSEKEEASRQVFEP"
     gene            complement(5426..7522)
                     /locus_tag="PK016_03660"
     CDS             complement(5426..7522)
                     /locus_tag="PK016_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HXL02201.1"
                     /translation="MNKKLLVVCFLSFFAIYSLLIVFSFDVGQLGELIGFYLFRFLGV
                     GAYILPFFLGYLAYEVYCFQPALKFRVMGRIISFSLWIVVFLTLSQRAAEKAGFSFPG
                     GDFAGIAGGFLYRHLVYYLGDTGSLLALFFVAFLAVYLGGEGKLLRQLSQGWRFLKRK
                     GEELFSARREVPQREVPPPRTSRKVKVQEKEEFISPRPEEVLDEIFAPLELETREEIQ
                     TKKKSTSSPKKKKSTTTWALPSPQLLKVYPSRGGEKTKKEIMEEIDKLEQTLAEFNVL
                     GKVISVQVGPTITRFEFQPAPGIKVQKITSLSNDLALAFAVAAVRIEAPIPGRSAVGI
                     EVPNRYKEIVSLRDLIESEEFKKNESPLTIALGRDVSGRPLIWDLREAPHLLIAGATG
                     SGKSVCINSILTSFLYRATPNDLRIGLVDPKRVELSLYSGLPHLCAPVISDARWVVRF
                     LKWLCREMDNRYELLSEVSARNIEEYNQMVEEKEKLPYVVIVIDELADLMMTAPAEVE
                     NSLCRIAQIARAVGMHLVVATQRPSVDVITGLIKANFPSRLAFAVSSQVDSRTILDSP
                     GAEKLLGNGDLLFAPLGASKPIRGQGAFVSTAETKKLVEYWLNQSGEIYYPLEYVPRE
                     ERGEGGGEEDDELYQEAVRVVIENGKASTSLLQRRLRVGFNRAARLIEQMEREGIVGP
                     YEGSKPRKVITPRGEG"
     gene            complement(7546..9681)
                     /locus_tag="PK016_03665"
     CDS             complement(7546..9681)
                     /locus_tag="PK016_03665"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HXL02202.1"
                     /translation="MTKQFEIGFGKESLLVETGKLAKQAGGAVTVRYGDTVVLVTATA
                     TEEPKEEVDFTPLLVEFEERFYAAGKIPGGFFKREGRPGQGAILNARLVDRSIRPLFP
                     ELFRNDIQVVVTVLSVDETHPPDTISILGASLALGISDIPFDGPLAACRMGLVGDELV
                     INPNLEELSSSKLDMIIAGSPEGITMIEAGALEVQEAQVAQAMMLGYQEITKVIKAQE
                     DIISQIGKTKREVIVPPYFKDLSFWLEDNYRASIREAITIPEKTEREKEAGLLMKKAQ
                     EEAMEKFESLSLFTVAWERIMREEVKELILKSERRQDGRTPKEIRPISCEVGFLPRTH
                     GSSLFTRGQTQALVITTLGASSEEQKVDALQEEEAKRFMLHYNFPPYSTGEVRPMRGP
                     GRREIGHGALAERALECFIPPEEEFPYTIRVVSEILESNGSSSMATVCGGSLSLMDAG
                     VPVRSSCAGLSIGLVKSEEKYLLLRDILGSEDHYGEMDFKVAGSRNGITAIQLDVKNR
                     GLSWEILGQALEEAKEGRFYILDIMDGAIDRPRSSVSPYAPRIEILDINPDRIGALIG
                     PGGKVIKKIVEESGATIDIKDDGKVIVFSRTEEGMNKAINMIRSITQDVEVGQVYLGR
                     VTRVTDFGAFIEIFPGREGLCHISQFSSERGKKVEDFVKVGDDVLVKVTGIDSMGRIA
                     LSHKEAVNNNDNSEKSNRGRESKHRSRER"
     gene            complement(9698..9967)
                     /gene="rpsO"
                     /locus_tag="PK016_03670"
     CDS             complement(9698..9967)
                     /gene="rpsO"
                     /locus_tag="PK016_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HXL02203.1"
                     /translation="MALTKERKQEIIEQFKIHEADTGSPEVQIAILTERIKDLTEHLK
                     THPKDFHSRRGLFLMVGQRRRLLNYLKKYHVDRYYAVLEKLGLRK"
     gene            10430..11839
                     /locus_tag="PK016_03675"
     CDS             10430..11839
                     /locus_tag="PK016_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HXL02204.1"
                     /translation="MASLKDNTQNISFFLEAKTPADIRKAILKLALPAMLEFMLMMLV
                     QMIDMIQVGSLGPWAIAATGLAAQPIMLIFSVFSALVVGTTALVARAAGAGDREYASR
                     VTRQSFVVVLLMGVIVTAVGIPASRTIIRLMGADVDTIDPGTIYMKIVMAGTVFTVGN
                     MVLAASLRGVGDMITPMISNIVANGVNIFMNWVLINGKLGFPRLEVAGAAIATSFSRL
                     IAFCITMHAVYARDKYISISIHDSYRFEPDVIRSVLNIGTPAAIEQLIMRTGALLFAR
                     LIAGFGTIMFAAHQIAIIIDGLGMVPAMAFQMATTTLVGQGIGAKKPELSEEATRQSI
                     IVSTIIMSLIGVAYFFLGKYAIMLFTRDTQVVALGENALKILAFSQPFTAAYFVVAGA
                     LRGAGDTKYAMYASAIGMWVTRLGIGYVLADLLNMALVGAWIAVTIDMAVRMVLVLRR
                     FSSGVVFKEYSKESLSEGT"
     gene            12261..13997
                     /locus_tag="PK016_03680"
     CDS             12261..13997
                     /locus_tag="PK016_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL02205.1"
                     /translation="MKKLLSYLKPYWKPTLLAPILMLIEVICDLSQPTLLASIVDNGV
                     AKSNLPYILNTGALMLGIALIGMAGGMGCSVASSIASQNFGASVRSAVFKKIQSLSPL
                     NIDRFKTSSLVTRLTNDITQLQMVVLMSLRIMVRAPLLFIGGIIMAVSLNARLTLILT
                     VSIPLLAAVIYYRMKKSMPLFSFMQQKLDRVNAVIRENLSGIRLVKAFVRADHEKKRF
                     ATANDELMHATLRAFNLIITMIPMVMLIMNFSIVAVLWFGGWDINRGNMEVGQIIAFV
                     NYITQILFSLMMIGNILLFVSRASASAERVNEVLDTDIDIKNADNPDFTPLSSGEVTF
                     EKVSFGYSGDKNQLVLKEISFHAPSGKTIAILGSTGSGKSTLVNLIPRFYDVTSGRVM
                     VDKRDVRSMDLATLRSGMGIVPQDTILFSGTIKDNIRWGKEDATDEEIIEAARVAQAH
                     EFIMSLPEGYNTQLGQRGVNLSGGQKQRIAIARAIIKKPPILILDDSTSAVDVVTEQR
                     IQKALKEFIRGSTIFIIAQRISSVMDADKILVLNEGRIEAEGTHEELLKSSPIYQDIY
                     NSQLGEGVMVDA"
     gene            13990..15804
                     /locus_tag="PK016_03685"
     CDS             13990..15804
                     /locus_tag="PK016_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL02206.1"
                     /translation="MHNPGRGHHDAVYVVKPKDAKNTLKRLWWYLSEHKIRLFLVFFL
                     VAITSMLTLMGPYLIGKAIDNYIIPRDFNGLFRLLMLMAAIYILMSLSTWLQTRTMIN
                     VAQLTIRNMRKDAFDKLEILPINFFDTRSRGDIMSRLTNDIDLINNALSSSLTQIFSS
                     IITLTGTVILMLWLSPLLTGVSMITVPIMLITTNIVTKHTRRYFSEQQRVLGILNGFA
                     EENISGQKVVKAFVRENKEIERFEATNKELKNVGIKAQIYSGIMGPLMNVLNNIGFAI
                     VALSGGWLAVQQVITVGTIAIFINYTRQFTRPLNELANQINTIQSAIASAERLFQIMD
                     ELPEPPDPPDAIELKSVKGDVEFRNVSFSYKPDEVVLKNINFHAYPGQTIALVGPTGA
                     GKTSIISLLTRFYDPDSGDIFIDGYNIQKITRKSLRSSLGIVLQDTYLFSESVKENIR
                     YGRLGATDEEVKAAARLANAEQFILSLPEGYDTILSEDGGDLSQGQRQLLAIARAILA
                     DPAILILDEATSSVDTRTEQHIQEAMLKLMEGRTSFVIAHRLSTIRKADMILVINDGE
                     IIERGNHTQLLKEKGFYYNLYMSQFAHILHQVEVCKTY"
     gene            complement(15940..17712)
                     /locus_tag="PK016_03690"
     CDS             complement(15940..17712)
                     /locus_tag="PK016_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007502864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HXL02207.1"
                     /translation="MSEMLFKKVDYMVVKLLEDIAVGEIGLPDIQRPFVWDTTRVRDL
                     FDSMYRGYPIGHLLFWENGFPGEHRLIGVDAKQKVPHLLIVDGQQRLTALYAVMKGVP
                     IRDKDFRERRVRIAFNPLEERFEVANTAIERDVSWIADISTLWKSDSYLYTFISKFLE
                     RLEQRQAVSEENRNRISQAIQHLSNLKNYPLTALEISMQATEEQVAEIFVRINSQGRT
                     LNQSDFILTLMSVFWDEGRKQLESFCYQAKYPPTDSRPSSYNHYFQPAPDQLLRVDVA
                     LAFRRARLEHVYSILRGKDLQTGEFSPARREEQFARLHEAQEYVLDLQNWHDFLHVLK
                     RAGYLHPSLISSQTAVVYTYALWLIGKRDFGLPFYALRDVMARWFFMASLTGRYTTSP
                     EARMEQDLALLRGCTRDYEFVKALEQEINAVLTSDYWEVTLPNELATASARNPAQFAY
                     FAALCILDAPVLYSTMKVRDLLDPTVQAKKKALERHHLFPREYLKNIGVQENRDINQV
                     ANYALVEWQDNINISNQPPSKYAPNAERRFSLEQLEEMYSFHALPEGWYDMSYFPFLE
                     ERRKRIAAIIRKGFQYLSDTGAVL"
BASE COUNT         5224 a         4180 c         3339 g         5071 t
ORIGIN      
        1 tcacccctta ttcacttttc aatgtgcgat tcatgtaggt aaaagaatat tttaatggta
       61 ccttacaatc aagtcaagat tcttccctat accatgcttc ctctatcact tcccaggaaa
      121 aaccccgacg aagaagacgg ttattaatgt cttctttttc tttcccctcc cctaaccatt
      181 ctttaattaa tcctcgagca tctcctacct cttcttcccg ggggaaaaat tctcgtagag
      241 actcaagcag ctcacgagag atacctctcc tgcgcaactc atccttcatg gtctgtaaac
      301 cccgggggtt cagttttctt cttgcctcta tataaacttt tacaaattct tcatcatcta
      361 agtagccgcg ttcttccaaa aactgaaccg ttttttctat ctcatctttc aggaaaccat
      421 ctcgggttaa tttccgagaa agttcatccc gggtatacat tttccgattg agatagcgaa
      481 aactttgttg gactatgtct ttcacgaatt aaaagcaact gaagggttaa cccccatctg
      541 agaaagagca gcggagagaa tttcttttgc tatttcgggg ttctctttca agaattgccg
      601 ggcgttatct ctaccctgac ctaaacgata ttccccaaaa gaaaaccagc tgcctgactt
      661 ttttatcact cctgttgtat ctcccagatt caatatttct ccttctcggg atatgccctc
      721 accataaatc aattctaatt ccgcctcttt aaatggagag gctactttgt ttttaaccac
      781 tttcactctg gttagagccc ctattatttc ttctcctgac ctcagataat ctcttcttct
      841 cacgtctaac cggacactgg cataaaattt gagagctctc cctcccggag tagtctccga
      901 aggtccataa gtcataccaa ttttttctcg gagttggtta acgaaaatag ccacagtttt
      961 agtcttgctg atataggcag tcaatcttct caaagcttga gacatcaacc gagcctgcag
     1021 gccaatgtaa gagtcagaca tgcctccttc taactccatt tgaggaacta aagcagccac
     1081 cgaatcaagc accactatat ccactccccc ggtgcgcacc aaggcatcta ctatatctaa
     1141 agcctcttct gcgcttccag gctgagaaat cagcagtttg tcaatctgga tcccaattct
     1201 acttgcgtaa ctagggtcta aggcatgttc tgtatccaca aaagcggcaa taccaccttg
     1261 cttttgagct tcagcaatgg catgcaaagc aatggtagtt ttacccgaag cttcaggacc
     1321 aaaaatttcc accactcttc ctcgaggaaa ccctcccacc cccagagcga tgttgagaag
     1381 aactgagcca gtctttatta cctctacatc agcagttaaa acatcttgag acagatacat
     1441 aatggcacct ttgccatatt ttctttctat agagcttata gcctggttaa tcacatcttc
     1501 tctcaaatcc ctagactttt tttcctcact catttttctt cacctcttcc aaactaaaag
     1561 cacctaattc ctgataaact gaacctcgag gggttaaggt actttgataa aaaaccagtt
     1621 ttttaatctc ggtagaaaat ctaatattct ctacctccag agaaggaaga gtaaaaggag
     1681 agcgaaagcg agccagagta atgtgaggag aaaactctct atcttccctt tcccatcccc
     1741 tgctaactat ttcctcttca acttcttctc ttatattttt aatcttttcc tctccctcct
     1801 ggtctactcc tatccatatc actcgagccc gatgaagaga agggaaggct ccccacctct
     1861 ctaaagttac agggaaggct gtaaatttgc tacttacctc ttttagtatt ctattcattt
     1921 cctccacccc accagaagga aaagcggaga aaaagtggag cgtaagatgg agttgctctc
     1981 ttttaaccca ttttgcttga gggtaaagtt ttttttcttg gttaattaac tcttccagaa
     2041 agtcctgagc ctccggtaaa aaatctaaag ctacaaaact tctaatggta actgaactca
     2101 tgaccaaatt tctcctttaa atactgaaaa accatcacca gagcagattg agaagcaaat
     2161 cttttattca tcagtctatc ccctgaaaaa tggaatttct gtactctaac accttcctca
     2221 ctcgataagg caatgtaggt caggcctact ggcttttctt tgcttcctcc tcctggacct
     2281 gcaataccgg taatcccgag acctaagtct acttttgtcc tctggcgtac cccgcgagct
     2341 aaaaattgag cagtttcttc actcactgct cctttttcct ttaaaatttc ctctggaacg
     2401 ctcagatcct ctttcttagc ctcgttacta tagaccacat atcctcgctc aaaatattga
     2461 gagctacccg ctatatcggt tattcgatta gctaccagac ctccggtgca cgactctgcc
     2521 aaccctaatt ttaagccttt ttccttcatc acattaccta ccgcttcttc caaagtatta
     2581 ccataaggac tgaagaaaaa tttatccagg tgatgagcta cctcatctaa aaaaagaagg
     2641 gcggattcct tttcctctgg agaaaattta gagctataga catagagcca cacctctcca
     2701 tagttgggaa gaaaagctaa attaaaagaa gagggtaaat gagagagaaa cggctctatc
     2761 tctttagcca gattagattc tccaatcccg caaaatttga agataggaga acgataaaaa
     2821 tattcctctt cttgaggaag gtctttctct atcaggctcc agaaaaactc tgcttcccgg
     2881 ggaacaccgg gtatgacaaa aatgcttttt ccatccactt ctaacttaaa accgggagca
     2941 gttccccgct cattataaag tatttctgcc ccttgaggga taaaagcctg ccttttatta
     3001 ttctcaggag gttcagtatt cctcagctcc cgatagaaaa actgaattct atcccaggct
     3061 tcagagctaa aaactaaacc tctaccaaaa gtttgagcta ctgcttcccg ggtcaggtca
     3121 tcttcagtag ggcctaggcc tcctgataca attaccagtt tcacttcttc ttctctcatg
     3181 agaaattgta aagcattcac tattttttct atctggtcag gcacaaaaag aataaattga
     3241 ggcaataccc ctttatctac caatttctga gccagaaagt aagcattgta atccctgacc
     3301 aacccggtca acagttctgt ccctatgcca agaatagcag ctttcaaatt ctttctcctt
     3361 tcaattcata gctactactt tcagtaactt taacttttac tatctctcct ctttttaact
     3421 tacttttccg ggagctaaag cttatcttgc catctacttc cggagcgtct ccgtaagtcc
     3481 ttccccaaca atgattaatt ccattctctc tttgaacttc atccactatt acttccaatt
     3541 cccgacccac taaagaacta tgaaactctc gcatcacttt ttcttgcgct ttcataagtt
     3601 ccttgtagcg ctcctgtttt actttctcct ccactttagg gtaaagggaa gctgaagata
     3661 catcgggtga ttcagaatag gtaaaagccc ccattcgctc aaagcgaaag tcttctacaa
     3721 aactcagcaa gcgctgaaaa gcttcttcgt cttcccctgg aaaacctact aaaaaagtag
     3781 ttcgcaaaaa tatatcgggg acactttccc tcaatttctc caatttttcc cttatttctt
     3841 ctatcgagga aggacgcctc atcctttgaa gaagagcaga atccacatgt tgaagaggga
     3901 tatcaagata ttttacaata tgagaagaat ccctcatcgc ttttataaat ccgcaatcca
     3961 gcccttcggg agaaaaatac aacaatctca accatattcc ggggggtaaa atttcatcta
     4021 accgacgaag aagaggtgat agcatcttcc gatgatagag gtctaatccg taagaagata
     4081 ggtcttgagc caccaggatt atttctttaa ctccctgagc caccaaatta agagcttctt
     4141 tttctatcga ctccatagtt ctactccgca aagaaccccg gatggagggt attagacaga
     4201 aactgcaccg atgattacaa ccttcagcta ttttgagata ggcatagtga gaaggagtag
     4261 aaagtactct ttctgtttcg tggttgtaaa caaaaggagg ggaatctaaa aagcatttcc
     4321 cgggaaattt atcttccaga acatcaacca gggaaggaaa atcattcact cctacccagt
     4381 aatctactgc aggatacaca aaactttttt ctctccccca gcgttgagca taacaaccca
     4441 taaccagcaa ttttttagga ggataagctt cttttagaaa agataatttt tcaatccatg
     4501 cttcagtttc ccgacaggct tcctcaataa aggcacaagt attgagtatc aatacctgtg
     4561 cttcttctgg agaagaaaaa aaattatatc ctcgagaaag caaagttccc agagctactt
     4621 cagcatccac taagttttta tcacaaccta agctaacaaa agcaattttt ttcacggctc
     4681 aaatacttgg cgcgatgctt cctctttctc actgagataa ccccggtctt ccccatttac
     4741 tgttgcttta actccattac ctccttttgc ttctacttct atagggtaat tgctttttat
     4801 cacatattct ttgttgggaa ccaatattcc agaaaaaact tcatttcctc ctgaccagat
     4861 ttctgcccag gccacttttt caggtaaaag agtaacggtg agagaaaatt gaggcccaac
     4921 ttctgcagaa ggagaaggaa aaatctcttc tggctcaatc gccgatactg gaggattaac
     4981 tactactact tctccctcct ctggcaaatg attaaaacta aagaaaatga aaagaaaagc
     5041 aaaaaacaca gctaaagaaa tcagaaccgc gagaagcctt ttaaataaag tagacctgct
     5101 gttccttcta tctttagctt ctctaagccc tggctcttcg gtttttttcc aatccagact
     5161 tttccaatcc acaggtttta ctgaataaac ttgattgaag agagaaacca tctcttcttc
     5221 attcaacccc agaaaacggc tgtaaatctt caaatatcct aaagcataag ctcttcctgg
     5281 caatttatcc cagttttcct cttccagagc agcaatatac cgagaggaaa tatgtgtagc
     5341 tccgcttacc tcgtcatagg tgagcttttt ttcttctctt ttttcccgca aaatctctcc
     5401 taatttaagt tgtccttcca ccattttatc cctctcccct gggagtaatt acttttcgag
     5461 gtttacttcc ttcataagga ccaacaattc cttctctttc catttgctca atcagacgag
     5521 ctgctcgatt aaaccccact ctcagtcttc tctgtaaaag agaagtggaa gctttaccgt
     5581 tttctattac cactctaact gcttcctgat aaagctcgtc gtcttcttct cctcctccct
     5641 cccctctttc ttcccgagga acgtattcca gaggatagta aatctctcca gactgattca
     5701 gccagtattc taccagcttt ttagtttctg cagtactgac aaaagctcct tgcccccgga
     5761 tgggcttact cgcccccagg ggagcaaaaa gcaaatctcc attacctaag agtttctccg
     5821 ctcctggact atccagaata gtccgggaat ccacctgaga agaaacagca aaagccagac
     5881 gggaaggaaa gttagcttta attaacccgg taataacatc taccgaaggt ctttgagtag
     5941 ctaccaccag atgcattccc actgcccggg ctatctgggc tatacggcac aaagaatttt
     6001 ccacctctgc aggagcagtc atcatcaggt cagccagctc atctataact attactacat
     6061 agggaagttt ttccttttct tctaccatct ggttatattc ctctatgtta cgagcagaaa
     6121 cttcagaaag aagctcgtaa cggttatcca tctcccgaca cagccatttg agaaacctta
     6181 ccacccatcg agcatcagaa atcactggag cgcaaagatg aggaagcccg gaataaagag
     6241 aaagctccac tcttttaggg tctacaagcc caattctcaa atcattagga gtagcccgat
     6301 agagaaaact ggtcaaaata gaattgatgc acacactttt acctgagcca gtagccccgg
     6361 caatcaggag atggggtgcc tctcgcaaat cccatatcag aggcctacca ctcacatctc
     6421 tacctaaggc aatggtgaga ggggattcat tctttttaaa ttcttcactt tcaatcaaat
     6481 ctcgtaaaga aacaatttct ttataacggt tgggaacttc aatccctact gctgaccttc
     6541 cggggatggg tgcttcaatt ctcactgcag caacagcaaa agccagggct aaatcgttag
     6601 aaagactggt aattttttgc actttaattc ctggagcagg ttgaaactca aaacgagtta
     6661 tggtgggacc tacctggacg cttatcactt ttcccagcac gttaaactca gccaaagttt
     6721 gttctaactt atctatttct tccattatct cttttttagt tttctctccc ccccgggaag
     6781 gataaacttt taaaagctga ggggaaggaa gagcccaggt ggtggtgctt tttttctttt
     6841 ttggagaaga agtggatttc tttttggttt gaatttcttc cctggtttcc agttctaacg
     6901 gagcaaaaat ctcgtccagg acttcctctg gacgaggaga gataaattct tctttctcct
     6961 gtactttcac tttccgggaa gtcctgggtg gaggaacttc cctttgagga acttctcttc
     7021 ttgctgaaaa gagctcctct ccttttctct ttaagaaacg ccagccctga gaaagctgac
     7081 gaagcaactt cccctcccct cccagataaa cagctaaaaa agccacaaaa aagagcgcca
     7141 gaagactacc ggtatccccg agataataga ccagatgacg gtagagaaat cctccggcta
     7201 ttccagcaaa atctcctccg ggaaaggaaa aacccgcttt ttctgctgct cgctgactta
     7261 aagtgaggaa aactactatc cacaaggaaa aactgattat ccggcccata actcgaaatt
     7321 taagagcagg ttgaaaacaa taaacctcat aggccagata acctaaaaaa aaggggagaa
     7381 tataggcacc cactcctaaa aagcgaaaaa ggtaaaaccc gattaactcc cctaattgcc
     7441 ctacatcaaa agaaaaaaca atgagcaagc tatatatagc aaaaaaactc aggaagcaaa
     7501 ctaccaaaag tttcttgttc actttgcttg actccgaagg tatatttacc tttctctaga
     7561 tcgatgtttc gattctctac cccggttgct tttttctgaa ttgtcattat tatttactgc
     7621 ttctttgtgg ctaagagcga tgcgccccat agagtctatt cccgttactt ttaccagtac
     7681 gtcatctccc actttgacaa agtcttccac ttttttccct ctttccgagg aaaactggga
     7741 aatatgacac aagccttctc tccccggaaa tatctctata aaagctccaa agtcagttac
     7801 tctggtaact cgacccagat acacctgacc tacttctaca tcttgagtga tacttctaat
     7861 catgttgatg gctttattca tgccttcttc agtacgagaa aaaactatca ctttcccatc
     7921 atctttgata tcaatagtgg ctcctgattc ttctacaatc ttcttgatga cttttcctcc
     7981 cggaccaatt aaggcaccaa tgcggtcggg gttaatatcc agaatttcta tccggggagc
     8041 ataaggagat acagaactcc gaggtcggtc tatcgctcca tccatgatat ccagaatgta
     8101 aaatcgccct tctttagcct cttctagagc ctgccccagt atttcccagc tcagacctct
     8161 gttctttaca tctagctgga tagcggttat gccgtttctg cttcctgcta ctttaaaatc
     8221 catctctcca taatggtcct ctgagcctaa aatatctcgc aagagaagat acttttcttc
     8281 cgatttaaca agacctatgg ataaaccagc acaagaagaa cgaaccggca ctccagcatc
     8341 catcagcgac aaacttcctc cacaaactgt agccatagaa gaagaaccat tagattccag
     8401 aatttccgaa accactctta tagtataagg aaactcttct tcaggaggaa taaaacactc
     8461 cagggctctc tctgccagag ctccatgacc aatttctctc cgtcccggac ctcgcatagg
     8521 ccgaacttct ccagtactat aaggaggaaa attatagtgc aacatgaacc ttttggcctc
     8581 ttcttcctga agagcatcta ctttttgttc ctcgcttgaa gcccctaaag tagtaataac
     8641 cagagcctga gtttgacctc gagtaaagag gctggagcca tgagtacgag gtagaaatcc
     8701 tacttcgcaa ctgataggtc ttatttcctt gggagtccga ccatcctgcc ttctttcact
     8761 tttcaagatc aactctttaa cttcctctct catgattcgc tcccaggcca cggtgaaaag
     8821 agataggctc tcaaatttct ccatcgcttc ttcttgagct tttttcatga ggaggcctgc
     8881 ttccttttcc cgttcggttt tctccgggat agtaattgcc tctctaatag aagcacgata
     8941 attatcttcc agccagaaag aaagatcctt aaaataagga ggaacaatta cttctctttt
     9001 agttttccct atttgagaga tgatgtcttc ttgagcttta atgactttag ttatttcttg
     9061 atagcccagc atcatagcct gagctacctg tgcttcttgt acctccagcg ctccagcttc
     9121 aatcatggta attccctccg gagagcctgc tattatcata tccagtttac tggaggataa
     9181 ctcttctaag ttgggattaa tcaccagctc atctcccact aaacccatac ggcaggcagc
     9241 taaaggccca tcaaagggaa tatcagaaat ccctaaagct aaggaagcac ccaaaatgga
     9301 tatcgtatca ggaggatgag tttcatccac tgaaagaaca gtaaccacta cttggatatc
     9361 attgcggaaa agttcgggaa aaaggggcct gatagaacga tctaccaggc gggcattcaa
     9421 aatagctccc tgtcccggtc taccttctct tttaaagaac cctccgggga ttttgcctgc
     9481 agcataaaaa cgttcttcaa actccacgag aagcggtgta aaatcaactt cttccttggg
     9541 ctcttctgta gcagtagcgg tcaccaacac tacggtgtct ccatatctga ctgtcaccgc
     9601 tcctccagcc tgcttagcca attttcctgt ctctactaat aaactttctt taccaaaacc
     9661 aatttcaaat tgcttcgtta tattaaacaa cctcccttta ctttcttaat cctaattttt
     9721 ctaaaacagc ataataacgg tccacatgat atttcttcaa ataatttaat aatctccttc
     9781 tctgccctac catcaaaaag agtccccgcc gagagtgaaa atccttagga tgggttttca
     9841 aatgctcggt gaggtcttta attctctcgg taagaatggc aatctgcact tcaggagagc
     9901 cagtatctgc ctcatgaatt ttaaactgtt ctatgatttc ttgttttctc tctttagtta
     9961 aagccaagta tttacacctc ccatttatac tccttcagta gtttatcaca ccctgactac
    10021 actgtaaata atagattgcc taacccctta aagaagcaat tttctttcag gagaaaaagc
    10081 aaaaactctc agtattaggt tataaaacca gataaaaaaa ccatcggttc caacaagctt
    10141 tagccttttc tctccccgct tttcagcgag aaacttcagg atggacttcc actccttaaa
    10201 gctttgagct tctcatcttt caaacagaag ctcttttttc catcctccgg cctagcttct
    10261 cagaaccgat ggcaataata gaatatcacc actttcaaaa tttgtcaata gaccgcgtca
    10321 agtattgata aaaagcgtta attcttgtat gataaaatta tgtagcactt ttaaccgagc
    10381 tcatgattgt gggatagtgt aatattttaa aaagcaaaag gtggttttta tggcatcatt
    10441 aaaagataac actcaaaaca tatcattttt tttagaagct aaaacgcctg ctgatatacg
    10501 taaagcaata cttaaattag ctttaccagc aatgcttgag tttatgctta tgatgttagt
    10561 tcagatgata gatatgatac aggtaggctc tctaggtccc tgggccattg ctgcaaccgg
    10621 gttagcagct caaccaatta tgctaatttt ttcagtattt agcgccctgg ttgtcgggac
    10681 tacagctctt gtcgcaagag ctgcgggagc cggagaccgt gaatatgcct ctcgtgtaac
    10741 cagacagtct tttgtagtgg tgctacttat gggagttatt gtaaccgcag taggtattcc
    10801 cgcttctaga actatcatac gattaatggg tgcagacgtt gatacaattg accccggaac
    10861 aatttatatg aaaattgtaa tggcaggaac agtgtttact gtgggcaaca tggtgttggc
    10921 agcttcttta agaggcgtgg gcgatatgat aacacccatg atttcaaaca ttgtggcaaa
    10981 tggcgtcaat atatttatga actgggtatt gattaatggc aaactcggat ttcctcgatt
    11041 agaagtggca ggtgcagcaa tagctacatc tttttccagg ttaatagcct tttgcattac
    11101 tatgcacgca gtgtacgccc gagataaata tatcagcata tctattcatg attcatatcg
    11161 ctttgagccc gatgtaatac gaagcgtgtt gaatattggc accccggcag cgatagaaca
    11221 gctgattatg aggactggtg cattattgtt tgcacgattg attgccggat ttggtactat
    11281 aatgtttgcc gcccatcaga tagcgataat tattgatggt ctgggaatgg tgccggcaat
    11341 ggcatttcag atggccacaa ctaccctcgt agggcagggt ataggcgcaa aaaaacctga
    11401 actttcagaa gaagcaacac gacaatcaat aattgtcagt acgataatca tgtcattaat
    11461 tggggttgct tactttttct taggaaagta tgccataatg ctttttactc gagatactca
    11521 agtagttgct ttgggggaaa acgctttaaa aattttagca ttttcccaac ctttcactgc
    11581 tgcttacttt gtcgttgccg gtgccctgcg gggtgcagga gatacaaaat atgccatgta
    11641 tgccagtgca attggcatgt gggtcacccg cttaggcata ggatatgtat tagcagattt
    11701 attgaatatg gcacttgttg gtgcctggat tgccgttacc atagatatgg cggtaagaat
    11761 ggtactggta cttagaagat tttcaagcgg cgttgtattt aaggaatata gtaaggagag
    11821 tttaagcgaa ggtacatagc tatacctctt tttgtcatcg ggagaggcaa acagccatgg
    11881 tgttctttct gcgaaatcgc tacaggtcgc aaaaaaatag cctatggcgt ttccccctct
    11941 ccttcatcac gaggagcgaa gcggcgaagc gatcccgagt ttttcaccta atgtaacctt
    12001 tgaagttcat tcaagaggga gattaccgcg tattgatgct ataagcttac cataacaata
    12061 ttcaaaaagg ggcatatcga aaacaggttg tgccgcaaaa atttagagtg cttacccctc
    12121 ttgcaaatat agggtataat tattcaccta gagcattttc tttttcttat tcatacagga
    12181 acttatttaa ccggtataat ataagaatga gacaatatat gtagataatt aagcgctgaa
    12241 caattttgcc aggaagataa atgaaaaagt tattatcgta tctgaaacca tactggaagc
    12301 caacgctgtt agctcctatc ttgatgctaa tagaagtaat atgcgattta tctcaaccca
    12361 ctttattagc cagcatagtg gataacggtg tagctaaaag taatttacct tacatattaa
    12421 ataccggtgc cctgatgctg ggcatagcct tgataggaat ggctggcggg atggggtgtt
    12481 cggtagcctc cagtatagcc agtcaaaact ttggagccag tgtgcgctct gctgttttta
    12541 aaaagatcca atccctttcg cctcttaaca tagatagatt taagacctca tcgctcgtga
    12601 cccgcctcac caacgacata actcaactac aaatggtggt tttgatgtcg ttgaggatca
    12661 tggtacgcgc accgctacta tttataggtg gcatcataat ggctgtatca ctaaatgcca
    12721 ggctaacgct tatcctcacg gtatctatac ccctactggc tgcggttatc tattaccgaa
    12781 tgaaaaaaag tatgccatta ttttctttta tgcaacagaa gctagataga gtcaatgctg
    12841 ttatcagaga aaacctttcc ggtatccggc tggttaaagc ctttgtgaga gctgaccacg
    12901 aaaaaaaacg gtttgccact gctaacgatg agcttatgca tgccactctt cgagctttta
    12961 accttattat aactatgata ccaatggtga tgctcataat gaactttagc attgtggcgg
    13021 tgttatggtt cgggggatgg gatataaatc ggggcaatat ggaagtaggc cagataatag
    13081 cttttgtaaa ttatataact caaatactct tctcgctcat gatgattggt aacatattat
    13141 tatttgtttc ccgcgcttca gcttcagcag aaagagtgaa cgaagtgtta gacacagaca
    13201 tcgatataaa aaacgccgat aatccggatt tcacgcctct atcttcagga gaggtaactt
    13261 ttgaaaaggt atcttttggg tatagcggag ataaaaacca gctggtgctc aaagaaataa
    13321 gttttcatgc cccatcgggt aagaccatcg ctattcttgg ctctaccggt tcaggtaaat
    13381 ccacactggt caacctcata ccccgttttt atgacgttac tagtggtcgg gtgatggtag
    13441 ataaaagaga tgtgcgttca atggatttag ccactttgag gtctggtatg ggtatagtac
    13501 cgcaggatac catattattt tcaggcacta taaaggacaa tattcgctgg ggtaaagaag
    13561 atgctacaga cgaggaaata atagaagcag ccagagtagc gcaggctcac gaattcataa
    13621 tgagccttcc cgaaggctac aatacccagt taggtcagcg aggtgttaat ttatccggag
    13681 gacagaagca aagaatagcc atcgctcgag caataattaa aaaaccaccc attcttatac
    13741 ttgacgatag caccagtgct gttgacgtag ttactgaaca gcggatacaa aaagcgctga
    13801 aggaatttat aagaggctcc actattttta taatagctca acgcataagc tcggtgatgg
    13861 acgccgataa aatattagta ttaaatgaag gccgcataga ggcagaaggc actcacgaag
    13921 agttgctaaa aagtagccct atatatcaag acatatataa ttcccagtta ggagaaggag
    13981 ttatggtaga tgcataaccc gggaagaggg catcacgatg cagtttatgt cgtaaagccc
    14041 aaagacgcga aaaatactct aaaaagacta tggtggtatt tatcagagca taaaataaga
    14101 cttttcctcg tcttcttcct tgtcgccata acatcaatgc taaccctgat gggaccttat
    14161 ctcataggga aagccataga taattatatc attccccgcg atttcaatgg attatttcgt
    14221 cttttaatgc ttatggcagc tatatacatc ctcatgtcct tatctacatg gttacagacc
    14281 cgcactatga ttaatgtagc ccagctcact atacgtaata tgagaaaaga tgctttcgac
    14341 aaactcgaga ttttgcctat aaactttttc gacactagat ctcgggggga tatcatgagc
    14401 agactgacaa atgatataga ccttataaat aatgctttaa gttccagttt aacccagata
    14461 ttttccagca ttattactct aacaggaact gtaatactga tgctatggct aagccctctt
    14521 cttacaggag tcagcatgat aacagtgcct ataatgttaa tcaccaccaa cattgtaacc
    14581 aagcacaccc gacgatattt ttcagaacag caaagagttt taggtatatt gaatggcttc
    14641 gctgaagaga atatatcggg acaaaaagta gttaaggcat ttgtccggga gaataaagaa
    14701 atagagcgat ttgaagccac taataaagaa cttaaaaacg tgggcatcaa agctcaaata
    14761 tattcaggca taatggggcc tttgatgaac gtattaaata atataggatt tgccattgta
    14821 gccctatccg ggggatggtt agcggtgcag caggtaataa ctgtgggtac catagccatt
    14881 tttatcaact ataccagaca atttacccgt cctctgaatg aattagctaa ccagattaat
    14941 accatccaat cagccatagc aagcgctgaa agactatttc aaataatgga tgagctacct
    15001 gaaccacctg accctcccga tgccatagag ctcaaaagcg tcaaaggcga cgtagaattt
    15061 cggaacgtat cattttctta taaaccagac gaagtagtgc tcaaaaatat aaatttccat
    15121 gcttatcccg gacaaaccat agccttagtt ggccccaccg gagcggggaa aactagcata
    15181 atcagcttac tcactcgctt ttatgacccg gatagcgggg atatattcat agatggatat
    15241 aatatccaga aaattacgag aaagagtctt cgctcatctt tagggatagt gttacaagat
    15301 acctatctct tttctgaatc agtaaaagaa aatatccgtt acggtcgatt aggtgccact
    15361 gatgaagagg taaaagctgc cgctcggtta gctaacgccg aacaatttat attaagttta
    15421 cccgaaggat acgatactat cctctccgaa gatggcggag acctgagcca gggtcagcgt
    15481 caactgctgg ctattgctcg agccatatta gccgacccgg ctatattgat actggacgaa
    15541 gccacgagct cagtagacac ccgcactgaa cagcacatcc aggaagctat gctcaagttg
    15601 atggaaggtc gtaccagttt tgtgatagct caccggctca gcaccatccg taaagccgat
    15661 atgatactgg tgataaatga tggagagata atcgagcgag gtaaccacac gcagttatta
    15721 aaagagaaag gcttttatta taacctgtac atgagccaat ttgcccatat ccttcaccag
    15781 gtagaagttt gcaaaacata ctagaatcct atttctagag caagataatc ataaaagctt
    15841 taagcattag ttccactgaa aatagatgaa tccagcccac ttcctaaatg ctgagagaca
    15901 aaaggattat aagctacaat aacattcact atcaaaggct tatagtacag cccccgtatc
    15961 agacagatac tgaaaccctt ttctaatgat agcagctatg cgcttacgac gttcttcaag
    16021 gaagggaaag taactcatat cataccatcc ttcaggcaat gcgtgaaagc tatacatttc
    16081 ctccaattgc tcaagtgaaa accgtctttc tgcattaggc gcgtatttgg atggaggctg
    16141 gttgctgata ttaatattat cctgccattc cacaagagca tagttagcca cctgattgat
    16201 atcacggttt tcctgaaccc caatgttttt aaggtactcc cgtggaaata aatggtgacg
    16261 ctctaaagct tttttcttag cctgaacagt cgggtcaagc aaatctcgta ccttcatcgt
    16321 agagtagagc actggtgcat ccagaatgca aagagcagca aaataggcaa actgagctgg
    16381 attacgagca gaagcagtag ctaattcatt tggtagggtt acttcccaat aatccgacgt
    16441 gagcaccgcg tttatttcct gttcaagtgc tttaacaaat tcgtaatccc tggtacaacc
    16501 acgtaataga gcaagatcct gttccatacg cgcttctggc gaggtagtat atcgtcccgt
    16561 caaggatgcc ataaaaaacc agcgagccat gacatcccgt aaagcataaa aaggaaggcc
    16621 gaaatctcgc ttaccaatta gccagagagc ataagtatac acaaccgcag tttgagaaga
    16681 aatcaggcta ggatgtaggt atccagcacg cttgagaacg tgtaagaagt catgccagtt
    16741 ttggagatct aagacatatt cctgtgcttc gtgcaggcgg gcaaattgtt cttcccgacg
    16801 cgcgggagaa aactcaccgg tttgcaagtc tttacctcgc aggatagaat agacatgctc
    16861 taatcgagca cgcctgaaag ccaaagcgac atctacccgc aataattgat caggagctgg
    16921 ctgaaaataa tgattgtacg atgaaggccg gctatccgta ggtggatact tggcctgata
    16981 acaaaaagac tccagctgct tacgcccttc atcccagaac acagacataa gggtaagaat
    17041 gaaatcagac tgattaagag tacgcccctg actgtttata cgcacaaaaa tttcagcgac
    17101 ctgctcttcc gtcgcctgca tggatatttc cagcgcggtc aagggataat ttttcaagtt
    17161 agacaaatgc tggatggcct gcgaaatccg gttgcgattc tcctcggaaa ctgcttgtcg
    17221 ctgctcaaga cgttccaaaa acttggaaat aaaagtatac agataggagt cagatttcca
    17281 aagtgtactt atatctgcaa tccaagatac atcacgctca atagcggtgt tggcaacctc
    17341 aaaacgctct tctaaggggt tgaatgcgat acggacacgg cgctcacgaa aatccttatc
    17401 tcttatagga acacctttca taactgcata tagtgccgtc agacgttgct gaccatccac
    17461 aattagcaga tgtggaactt tttgcttggc atctacacct ataagccggt gttcgccggg
    17521 aaaaccattt tcccaaaaaa gcaaatgacc gatgggataa ccacgataca tagaatcaaa
    17581 aaggtcacgc acgcgggtgg tatcccacac aaaggggcgc tgaatgtcag gtagaccaat
    17641 ttccccaacg gcaatatctt ctagcagttt aactaccata taatccactt tcttaaaaag
    17701 catctcactc atacttttcc ctccaaactt ggcaacaaac ttgcatcaat atccacgctt
    17761 ttgtaaccat caaacgttta cataatccta aagggcacat ccagcataac cagt
//
LOCUS       DAOSVE010000027        16859 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_2109_length_16859_cov_68.262676, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000027 DAOSVE010000000
VERSION     DAOSVE010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 16859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16859
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2109_length_16859_cov_68.262676"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(349..783)
                     /locus_tag="PK016_03695"
     CDS             complement(349..783)
                     /locus_tag="PK016_03695"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HXL02208.1"
                     /translation="MVNTFKVTGEWKKGLRIDTRVRNFALSFDEPPSLGGEDSACNPV
                     EGLLASLAGCLGIVSRVVAQEKNLPIEKIEVEVEGDLDPRGFMGQYDQVRPGLLAIRY
                     QLKVSGNLSDKELKEFREEVDKRCPVSDTLKNGTKVEGKVTL"
     gene            complement(799..1386)
                     /locus_tag="PK016_03700"
     CDS             complement(799..1386)
                     /locus_tag="PK016_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013683044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HXL02209.1"
                     /translation="MEEKKIWYFPYPGPDNTSVTLDIALERAKELGIKYIVVASRSGD
                     TALSLERKAREVTYSGNLIMVTYHAGFYGGDSISFTPSRKEELEEKRVKIVMGSHSLS
                     GVSRSFRQKFGGVSIPEIIAESYRRISQGFKVAVEVAIMAADGGAIPTTEDVVSMGGN
                     SKGVDTAIVLRAAHQNSFFDLKIKEILCLPREEKD"
     gene            complement(1400..2062)
                     /gene="phoU"
                     /locus_tag="PK016_03705"
     CDS             complement(1400..2062)
                     /gene="phoU"
                     /locus_tag="PK016_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HXL02210.1"
                     /translation="MEIVRERFTQQLKELQEDLVSMTHAAEKMLQLSIESLKERNVEK
                     AQEAIVLDDVVDDFNFKIEERCLSLIALQQPVAKDLRIIAAILKVITDVERIGDYSVD
                     IAKFSIRLVEKPLFKPLIDVPRMADLVKGMLEEAITAFIQRDLTIVQKVVEADNEVDE
                     LYRSVHEEVVENIERDPSVTRQAIWVLMIARYLERIGDHVTNITERIYYMETGELIEL
                     HQ"
     gene            complement(2089..2568)
                     /locus_tag="PK016_03710"
     CDS             complement(2089..2568)
                     /locus_tag="PK016_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXL02211.1"
                     /translation="MKVGILSDSHDNLLLLKRALDILEKEKVDLILHAGDYVAPFSVE
                     LLTRYSIPWEGVLGNNDGEVLGIFRKSGGKVNSPFWEGEKNNFRIWVSHFYLPAELAF
                     ESRKYNLVVYGHTHEAVIKKEDDFFLINPGEVCGLLSGKPTLALCDLEKKVAKLIDI"
     gene            complement(2581..3075)
                     /locus_tag="PK016_03715"
     CDS             complement(2581..3075)
                     /locus_tag="PK016_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HXL02212.1"
                     /translation="MEVLEVLKKRRSIRQFKNLPVEREKVEEIIDCARLAPSGNNFQP
                     WEFIVVTDHLKRREIANVADYGKFITQSPVCIAVFCRDTRFYLEDGCAATENILIAAR
                     ALGLGSCWIAGDKTPYALQVAKILGVPANYHLVSLIALGYPEAEPAPPQKRSLEEVLH
                     WEKF"
     gene            complement(3088..4167)
                     /locus_tag="PK016_03720"
     CDS             complement(3088..4167)
                     /locus_tag="PK016_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HXL02213.1"
                     /translation="MVRRAFADLPLHGGKCPPWLFERMKVLGGLIIEAIVEFFGPEEV
                     LRRVSDPVFFQSLGCLLGFDWHSSGLTTTLCGALKEGVRGREKDLWLFICGGKGATSR
                     KTPEEIYIHAEKNALPFADQLVYLSRMSAKVDNSALQDGYNLYHHSFFFTSSGKWAVV
                     QQGMNPENKYARRYHWWGEGVKDLVCEPHQGIWGEKREEEVLNLVARESTDCRNGMVE
                     LVQEKPEKLKKDMALIPRFSLPSRHFIKEGDFNLGRIDKTLQMIYEKKPQNFENLLAL
                     PGVGPKTLRALALTTEVVMGAPPSFTDPVSFSFAHGGKDGIPFPVDRETYQETIEFFT
                     KVVQRSHASSTDKKKMYEQLKLLMR"
     gene            complement(4180..4662)
                     /locus_tag="PK016_03725"
     CDS             complement(4180..4662)
                     /locus_tag="PK016_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HXL02214.1"
                     /translation="MEVKIVKIENPQGYNLILGQAHFIKTVEDLYETLITSSPHLKFG
                     LAFCESSGPCLVRYAGNDEELTELAKSNAWNISAGHTFIIFLKEGYPINVLNAVKMVP
                     EVCHIYCATANPLEVVIVETEQGRGVMGVVDGFKSEGIEGEKEIADRKRLLRQIGYKL
                     "
     gene            complement(4666..7257)
                     /locus_tag="PK016_03730"
     CDS             complement(4666..7257)
                     /locus_tag="PK016_03730"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, SERCA-type"
                     /protein_id="HXL02215.1"
                     /translation="MVKKGVNWHELSIEEVVKILDSDEKEGLGEEEAKQRLTKYGRNA
                     LPQTKKKTVFTMFLEQFKDFLVLILVGAALVSLLLGETTDSLVIIAILILNAVLGVVQ
                     ESRANQALEALKKMTVPECEVIRGGKPHKISSEEIVPGDIVVLREGDFVPADLRLIEI
                     HALKIDESSLTGESVPVEKNIAPLPADAPITDRLNMVYSGTLVTYGRGKGIVVATGMD
                     REIGKIASLIQEEEETVTPLQKRLAGLGKLLGFLTLGICALVFLVGFWRGEPPFGMFM
                     TAVSLAVAAIPEGLPAIVTVVLALGVYRMSQHRAIIRKLPAVETLGSTTYICTDKTGT
                     LTENRMVVREIWTESEEKLYQIAVLCNDALLEDREGEEVRFGDPTELALLDYVKKQGF
                     DFSSWRKIYSRQGEVPFDSNRKMMSTLHNMDEETVLLVKGAPDIVITHCSFYEREGEI
                     IPLSSEKEQEIRKVMEEMAGKALRVLAFAWREMEEGITPSPEEEKDLIFVGFMGMIDP
                     PRPEARSALEEANKAGITTVMITGDNPLTARAIAEELGMFSPQNLVITGPELANFSPE
                     ELKANIKKIKVFARVWPEQKLKIVEALQANEEVVAMTGDGVNDAPALKKADIGVAMGV
                     SGTEVAKESADMILTDDNFATIVHAIKEGRVIFDNIRKFVVYLLSCNLGEIFAIFIPI
                     LVGWHSPLVPVQILLINLVTDGLPALALGMDSPEPDVMMRKPRHPREGIVNSHYMRIV
                     LFNAFFITLAVVASFGMGLNLWGRELSQTLAFATLGFDELWRAYSFRSEKRNFWQINP
                     RGNLYLVGACLLSFLVIILVILLPPLQRIFQTASLSLAQWIWVILFSLIPVSAYEIRK
                     LFHKKGE"
     gene            complement(7239..8057)
                     /locus_tag="PK016_03735"
     CDS             complement(7239..8057)
                     /locus_tag="PK016_03735"
                     /inference="COORDINATES: protein motif:HMM:NF024622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HXL02216.1"
                     /translation="MPIIGKDEQKVKAFGNVSKRLSLSNRVYRGMQTIPVNKIVGSVG
                     RVADLLPGFRLKNPDARYYRIRKAMERGEVLPPIIVYQVGDEYYVLDGNHRVAVAKDL
                     GVKYIDAEVIEFFPSELRESRALRKKRAEFESLTGLKGIDASEPRHYDSFLSYIQDYA
                     KRMEAFLGREVPLKEASLNWFLSVYTPAVQAIVERGLEEAFPDKSLADIFAYVLDYKW
                     YKSQKEGYDIGFEQALEGFIQEIVGKKESEEDRKTLMQRVGGLLEEVLPWLRKE"
     gene            complement(8069..8791)
                     /locus_tag="PK016_03740"
     CDS             complement(8069..8791)
                     /locus_tag="PK016_03740"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HXL02217.1"
                     /translation="MVNLNVLISFLAGLLSFLSPCVLAIAPAYVSYISGVESTEQEKS
                     KLLTHTLLFVTGFSGVFILMGLGASFLGSVLLPYRVWFNRLAGAIIVIFGLQVSGVLK
                     IRWLYADKHFRPKEMALSRVRSLVLGVSFGLGWTPCVGPILGSILLYASSMGQVWQGG
                     VLLFFYALGLALPFIFLGIGWGRLTTFFRGLQKRGRWVEIISGILLVFLGVVIFTGEM
                     NTLFSLLGVDASFNPELLLLRE"
     gene            9005..>10251
                     /locus_tag="PK016_03745"
     CDS             9005..>10251
                     /locus_tag="PK016_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HXL02218.1"
                     /translation="MYWEKQLETIPREELSLLQAERLQKTIINAQKTSFYRRLFARNN
                     IDPEKIKSPEDLKVIPPTTKADLREGFPFDFLAVPLEEVIRLHSSSGTTGTPTVIYYT
                     ASDLDRWTNQVARSLYAVGMRKNDVFQNMMSYGLFTGGLGMHYGAERLGALTIPIGAG
                     NSKRQIWFLKFFKSTAIHIIPSYALLLAQLIKAEGLDPHQDFNIRIMLLGAEPHSEET
                     RLRIEEIYGAKAYNSYGLSEVNGPGVAFECVFQEGMHLWEDYYILEILNPQTLSPTPL
                     GEVGEIVLTTIYRDATPLIRYRTGDLAFLLEEPCPCGRTHQRISRIQGRTDDMFIFRG
                     VNIFPMQIEKVLMSNPEVGNNYRIILEREDYRDKMMVEVEIKPELFSGDLQKLARLKE
                     RIQSELHSEILVTPEVVLVEPGS"
     assembly_gap    10252..10351
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10352..10796
                     /locus_tag="PK016_03750"
     CDS             <10352..10796
                     /locus_tag="PK016_03750"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HXL02219.1"
                     /translation="KGGNKMARQLSIFAENKPGRIEKITRLLSQENINVRAITISSAN
                     GFGVIKILANEPQKAFDLLRDNNFPVYLQEVIAVVMNDEPGGLHRVAQTLSANQINIE
                     DAYGFVIKSGEKAVLVIQVENQPQAHSILERNGFQLLTDEEIYQL"
     gene            11102..11488
                     /locus_tag="PK016_03755"
     CDS             11102..11488
                     /locus_tag="PK016_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02220.1"
                     /translation="MKRVVIIVMLLVTTILAGCTEEVSGKQSPEELKYDLNFLIQTLE
                     DVHPDLYRYTPKEEIYKMKQDIEEYLSEPLTRLEFYRKVAPLVTRIGDGHTYITYPVE
                     ELNEYINEGGVFSRSRSESLMKDYLS"
     gene            11725..12603
                     /locus_tag="PK016_03760"
     CDS             11725..12603
                     /locus_tag="PK016_03760"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HXL02221.1"
                     /translation="MTLNELTRRAQELQKESTKKEEVKEEDRTKIQYPYYSYTSVSEL
                     KTGVIDFRAFQNPEQFNSFLEETFRKIKEEEIEYLIIDIRNNSGGNSTLGDALLDYLT
                     DKPWRQFSKIELKVSKQIKEYYQDPLPVLKKIYPESYESAYQTSREWYQSVYQEIASL
                     PEGEISFSSPEFTEPSENPLRFYGKLYVLIGSHTFSSAVNFAATVKDYGIGTLIGEET
                     GGLATQFGDVYPFWLPNTDLSAGVSHKKFYRPSGIDDGRGVLPDIEVTTTLEDVLKGK
                     DPVMEQVLEIIKSKVL"
     gene            complement(12644..12720)
                     /locus_tag="PK016_03765"
     tRNA            complement(12644..12720)
                     /locus_tag="PK016_03765"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12684..12686),aa:Arg,seq:acg)
     gene            complement(12773..14101)
                     /locus_tag="PK016_03770"
     CDS             complement(12773..14101)
                     /locus_tag="PK016_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02222.1"
                     /translation="MNKKTIFFIVVGILVLGGSISWAQAPLFREQLVYSLTSFNGSGF
                     SSSFCPQSEGTLYFIANFNSVVSPRKTIVYYWPITRRYMAGFSTLNEEVPGKLEIWQG
                     GKLINTLDKQKYVLYYPEGYWSEKATIYLDDEAQKYYDEYKAAVDEFNKKLQEYYQAM
                     NYYQQQLNNFFEEVRRRREAGDTGPLDIPIPQQPEPPEFVKFYVTEPAEGFILNLPVG
                     NYEIKVRMEDGTIFEGSEKNLIVFTSRRREGVGYEIIPGNRWTKRENCDDPSWIIHAV
                     GKNELYFNPFYQEEYNELYYNKLQDPQNIGRLESWKWVHVDPVNNAQIILGRREEILQ
                     TVDKAPYFVRQVSGPELGYEILPYDEELKKSGYQPTFESYKVTLGEDLPRADYQIYTI
                     SRETGEHIPKSERTIRFLKKENGAYLYAVAFLPLVVGAIAIIGRSYRVEK"
     gene            complement(14101..14919)
                     /locus_tag="PK016_03775"
     CDS             complement(14101..14919)
                     /locus_tag="PK016_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXL02223.1"
                     /translation="MAGKVTKTLFKRDFLYTLYSWGYYAAIFVSFLVSSFILKNFIDG
                     IREEDILVSAYPLHQPLYITLVIISIYLVIVSAISISREREQGTLEVLFYGPVTAGNF
                     LWSKYFKDLLLGIIALCFTAVYFYLVSYFTNLGFTIGLVKALFMGIFLISCVVSFGLF
                     ISSLTGRIRSSVITLLAILGAFLAIQFAYGALLGLGEEATISLPLLYLRQTLSYVFQG
                     INWISPFAFLNRGLDAVVLESWPLYLTNIAYCLIYSLIFMVLSIFIMNRRGVKA"
     gene            complement(14922..15644)
                     /locus_tag="PK016_03780"
     CDS             complement(14922..15644)
                     /locus_tag="PK016_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02224.1"
                     /translation="MKKKAGLSIFLSIVGVIVVLFVVSYFTVRLNYGILQGKVTDYYS
                     QDVVRKLHLTVDGRSDILFQSKDYQFTHLSPGKYTLQAEAPYYYPFSEELEIKRGVNV
                     FNFTMKGKEIPDLAGIICFADPVDRGIQIEIRFKDSNGTGISDFPALPLQLEGRLYVR
                     EGEDENNYSRGRLIFEGPIELFWDPDAYLSRNKGIITWDKIQIDPEKEKYGIMELILH
                     TPQGDFEDVIEDVELQLPKKEE"
     gene            complement(15641..16588)
                     /locus_tag="PK016_03785"
     CDS             complement(15641..16588)
                     /locus_tag="PK016_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL02225.1"
                     /translation="MIQTINLTKEFDGFKAVDNLNLQVDKGDIYGFLGPNGAGKTTTI
                     NMILGLIPPTSGKIFLLGEEFYPGKVEVKRKIGVVSESLYFYKEMTAREYLLFFAELY
                     QVPQVSKRIGELLEALGLVNDQNKKVGAFSKGMQQKLAFARALLHDPELLILDEPVAN
                     LDPNSIKQIRDLIEDENHRGKTLFISSHLLSEVERLCKKVAIINKGRLLAEDTMDNIR
                     RRLTDAVEMEIELDKEAGDLASSLEKLDFLKGFNIEGHKLEVNLKTDRDYRPELSQFL
                     YRNGYLPLSMKAKEMSLEEAFITITQQNISLLARGEEAA"
BASE COUNT         5196 a         3861 c         2898 g         4804 t
ORIGIN      
        1 cgtagcgacc ttgagttttt gactttcgat ataatttact tcctaaagtt tttaaccagt
       61 tttgcgggat actataatct tgtagactgt cttgcgacat ttcccccatg tcagtgagtt
      121 cttttcctct cctggtagct ttttatattt ctcttgtgag agccatagaa attatcccac
      181 ccattgcttt cttatttcac tcccaatatt tagccggtaa tctattttct tctatcattc
      241 ccaaatgttt aaatcgggaa tctaaatact actggtagga ttttcctacc agtagtattt
      301 gtttttcatg tttttttata tttttatctt acgtcgtagg ctgtcctgtt atagagtaac
      361 tttgccctct actttagtac cattttttaa agtatcagaa actggacaac gtttatctac
      421 ttcttctctg aattctttaa gctctttatc gctgaggtta ccacttactt tgagctggta
      481 gcgaatagcc agcaatcctg gacgaacctg gtcatattgt cccataaaac cccgggggtc
      541 taaatctcct tctacttcca cctctatttt ctcgatgggt aaattcttct cctgagctac
      601 cacccggctg acaatcccta aacacccagc aagagaagcc agtaagcctt ccaccgggtt
      661 acaggcacta tcttctccac ctaaagaggg tggctcatca aaggaaagag caaaattcct
      721 cacccgggta tctatccgta aacccttttt ccattcccca gtaaccttaa aggtattaac
      781 cattaaaatc tctcctttct aatctttttc ttctcgaggt aaacaaagta tttctttaat
      841 tttcaaatcg aagaaagaat tttgatgagc agccctaaga acaatagcag tatccactcc
      901 cttactatta cctcccatag acacaacatc ctcagttgtg ggaatagctc caccatcagc
      961 agccataata gctacctcca ctgccacttt aaagccttga gagatgcgtc gatagctctc
     1021 ggcaataatt tcagggatgc tcacccctcc aaatttttgt cggaaagagc gacttactcc
     1081 acttagggaa tgggagccca tgacaatctt cactctcttt tcttctagct cctctttacg
     1141 ggaaggggta aaagatatac tatctccgcc atagaaacca gcatgatagg taaccataat
     1201 caaattacca gaataggtta cttcccgggc tttcctctcc agagataaag cagtatctcc
     1261 agagcgagaa gctactacga tatattttat ccccagctct ttcgcccttt ctagagcaat
     1321 gtccagggtt actgacgtgt tatccggacc aggataagga aaataccaga tttttttctc
     1381 ctccattttt cctctccctc tactgatgta gttctatcaa ttcgccagtt tccatataat
     1441 aaatacgttc agtaatattg gtaacatgat ctccaattct ttctaaatag cgagcaatca
     1501 ttaaaaccca gatagcctgg cgagttacac tggggtctct ttctatattt tctactactt
     1561 cttcatgaac agaacgataa agttcatcca cctcgttatc ggcttctact actttttgca
     1621 cgatggttaa atctctctgt ataaaagcag tgatagcttc ttctaacatc cctttaacca
     1681 ggtctgccat tctgggtaca tctataagag gcttaaataa cggtttctct actaagcgaa
     1741 tactaaattt ggcaatatca acagaataat cgccaatcct ctccacatcg gtaataactt
     1801 ttaatatagc agcgattatt ctcaaatctt tagctaccgg ctgttgcaaa gcaatgagtg
     1861 ataagcatct ttcttcaatc ttgaagttga agtcatccac cacatcatct aaaacaatag
     1921 cttcctgggc tttctctaca tttctttcct tcagtgattc aatagataat tgtaacattt
     1981 tttctgcagc atgggtcata ctcaccaaat cttcttgtaa ctcttttaat tgttgagtga
     2041 aacgctccct aacgatttcc attatcgtcc cccttcctct ctattaaatt agatatcaat
     2101 taattttgcc acttttttct ccagatcgca aagagccaga gtaggctttc cactgagtaa
     2161 accgcacact tcacctggat taatcaagaa aaaatcatct tccttcttta tcactgcttc
     2221 atgagtatgt ccataaacta caagattata ttttctactc tcaaaagcta actctgccgg
     2281 taaataaaaa tgggagaccc agatgcgaaa gttgtttttc tctccctccc agaaaggaga
     2341 atttactttc cctcccgatt tccgaaaaat gcccagtact tccccatcgt tattgcccag
     2401 cactccctcc caggggatag aatagcgagt aagaagctct accgaaaaag gagcgacata
     2461 gtccccagca tgaaggatga ggtctacttt ctctttttct aaaatatcca aagcccgctt
     2521 gagtaaaaga agattatcgt ggctatcaga taaaataccc actttcattt ttaatctccc
     2581 tcaaaatttt tcccagtgta aaacttcttc taaacttctc ttttgcggtg gggcaggttc
     2641 cgcttcagga tatcctaaag ctatcaagct cacaagatga taattagctg gaacgccaag
     2701 aattttagct acctgtaaag cgtaaggtgt tttatcaccg gctatccagc aggagcctaa
     2761 acctaaagct cgggcagcaa tgagaatatt ttctgtagca gcgcaaccat cttccagata
     2821 gaagcgagtg tctcggcaaa atactgctat gcaaaccgga gactgagtaa taaactttcc
     2881 gtaatcggca acattagcaa tttcccgccg cttgagatgg tcagtcacca ctataaattc
     2941 ccagggctga aaattattac ccgaaggagc caagcgggca caatctatta tctcctcaac
     3001 tttttctctt tccaccggca aattcttaaa ttgacggatt gagcgtctct tctttaatac
     3061 ttccagaact tccatatttg tttcctccta tctcattaaa agtttaagct gctcatacat
     3121 tttcttttta tcagtagatg aggcgtggga acgctgcacc accttagtaa aaaactctat
     3181 agtttcctgg taggtttctc ggtccaccgg aaagggaatc ccatcttttc ctccatgggc
     3241 gaaagaaaaa ctcaccgggt cagtaaagct gggaggagct cccatcacca cctcagttgt
     3301 gagagctaaa gcccgtaaag ttttagggcc caccccggga agagctaaaa gattttcaaa
     3361 attctggggc tttttttcat aaatcatctg taaagtttta tctatccttc ccagattaaa
     3421 atctccttcc tttataaagt gacgggaggg aagagaaaag cgaggaataa gagccatatc
     3481 tttttttaat ttctcgggct tttcctgcac cagttctacc ataccatttc tacaatctgt
     3541 actttcccgg gctaccagat tgagaacttc ttcttccctt ttttctcccc aaattccttg
     3601 atgaggttcg caaactaaat cttttactcc ttctccccac cagtgatacc gacgggcgta
     3661 tttattctca ggattcatac cctgttgcac caccgcccac tttcctgaag aagtaaaaaa
     3721 gaaactatga tgatagagat tgtaaccatc ctgcagagct gaattatcca ctttagccga
     3781 cattcgactc agataaacca gctggtcagc aaaaggaaga gcattttttt cagcatggat
     3841 atagatttct tccggggttt tgcgagaagt agctcccttt cctccacaaa taaaaagcca
     3901 taagtccttt tctctgcccc tcactccctc cttcagcgct ccacacaaag tagtagtcaa
     3961 gccactggaa tgccagtcaa atcccagaag gcaacccaga gactggaaaa aaaccgggtc
     4021 agaaactcga cgcagcactt cttccggtcc gaaaaattct actatggctt caataattaa
     4081 gcctcccaga actttcatcc gttcaaaaag ccagggagga cacttccctc catgaagagg
     4141 aaggtcagca aaagctctcc ttaccaccac accactcctt taaagtttat aaccgatttg
     4201 ccgcaatagc ctcttgcggt cagcaatttc cttttctccc tctatgcctt cacttttaaa
     4261 cccatctacc actcccatta ctcccctacc ctgctcggtt tctactatta ctacctccaa
     4321 gggattggca gtagcacaat atatgtggca cacctcagga accattttca ccgcgttaag
     4381 aacattaata ggataacctt ctttcaaaaa aattataaaa gtgtgtcctg ccgatatatt
     4441 ccaggcatta ctctttgcta actcggtaag ttcttcatca ttcccggcat aacgaactaa
     4501 gcacggtcct gaagactcac aaaaagcaag gccaaacttc aaatgaggag aggaggttat
     4561 caaagtctcg taaaggtcct ccacagtctt aataaaatga gcctgtccca aaattaagtt
     4621 ataaccctgg ggattctcaa ttttcactat ttttacttcc atattttatt cccccttttt
     4681 atggaatagt ttcctgattt cataagcact aacaggaata agggaaaaaa gaataaccca
     4741 aatccactga gcaagactta aacttgccgt ctggaaaata cgttgcagag gaggtaaaag
     4801 tatcaccaat attataacca gaaaagatag taaacaggct cccaccagat ataaattacc
     4861 tcgaggattg atttgccaga aatttctctt ttctgaacga aaactataag ctctccataa
     4921 ctcatcaaaa cctaaggtgg caaaagctaa ggtttgactt agctctcgtc cccataaatt
     4981 taatcccatt ccaaaagaag ccactactgc taaagtaatg aaaaaagcgt tgaacagcac
     5041 aatcctcata taatgcgaat taacaatccc ctctcgagga tgacgaggtt tacgcatcat
     5101 tacatctggt tctggactat ccatccctaa agccagagcc gggagaccat cggtcaccaa
     5161 gttaatgagg agaatttgta ccggtactaa gggactatgc caccccacca aaatgggaat
     5221 aaaaatagca aatatctctc ccaaattaca ggataaaaga taaactacaa attttctaat
     5281 attatcaaat atcaccctac cttctttaat agcatgcaca atggtggcaa agttgtcatc
     5341 agttaaaatc atatcggcgc tctctttggc tacttccgta cctgaaacac ccatagccac
     5401 accaatatca gcttttttaa gagccggagc atcattaacc ccgtcaccag tcatagctac
     5461 tacctcttca ttagcttgta gagcttccac aattttgagc ttttgctctg gccatactcg
     5521 agcaaacact ttgatttttt taatatttgc ttttaattcc tccggagaaa aattagccag
     5581 ctccggtcct gtaatcacaa gattctgagg agaaaacatt cctaactctt ctgctattgc
     5641 ccgagcagtg agaggattat caccagtaat cattactgta gtgataccag ctttatttgc
     5701 ctcttctaaa gctgacctcg cttctggccg aggggggtca atcataccca tgaaacctac
     5761 aaaaattaaa tctttttctt cttccggtga aggggtaatt ccttcttcca tttctctcca
     5821 ggcaaaagct aaaacccgga gagcttttcc agccatttct tccatgactt tccttatttc
     5881 ttgctctttt tctgaagaaa gtggaataat ctctccttct ctttcataaa aagagcaatg
     5941 agtaataact atatcaggag cacctttaac taagagcact gtttcttcat ccatattatg
     6001 aagagtggac atcattttcc ggttagaatc aaaaggaact tccccctgac gagaataaat
     6061 tttcctccag gaggaaaaat caaagccctg ctttttgaca taatctaaaa gagccagttc
     6121 ggtaggatct ccaaatcgca cctcctcgcc ctccctgtct tcaagtagtg catcattaca
     6181 aagaacagca atttgataaa gtttttcttc agactccgtc catatttctc gaactaccat
     6241 ccgattttca gtaagagttc ccgttttatc ggtacagata taagtagtag aacccagggt
     6301 ttctacagca ggcagtttac gaataatagc tcgatgctgg ctcatacgat aaactcccaa
     6361 agccaggact acagttacaa tggctggcaa accttcagga atagccgcta cagcaagact
     6421 tactgcagtc ataaacatac caaaaggcgg ttccccgcgc caaaagccaa ctaaaaaaac
     6481 gagagcgcat attcccaggg tcaaaaagcc caggagtttt cccaaaccag ccagtcgttt
     6541 ttgcaaagga gttactgttt cttcttcttc ctggattaag ctggcaattt tccctatctc
     6601 tctatccata ccagtggcta ctactattcc cttacccctc ccgtaggtaa ccagagtccc
     6661 agaataaacc atattaagac ggtcagtgat aggggcatca gcaggaagag gagcaatatt
     6721 tttctccacc ggtaccgact caccagttaa gctcgactca tcaattttca aagcgtgaat
     6781 ttctataagg cgcagatcag ccggaacaaa atctccttct cggagaacga ctatgtctcc
     6841 aggaactatt tcttctgagc ttatcttatg aggttttcct cctcttatca cttcgcattc
     6901 cggaacagtc attttcttta aagcttccag agcctggtta gctctacttt cctgcactac
     6961 tcctaaaaca gcattcaaaa tcaaaatagc aataattacc aaagaatcgg tagtctctcc
     7021 taaaagaaga gaaactaaag ctgcccccac taaaattaat accagaaaat ctttgaactg
     7081 ctccaaaaac atagtaaaga cagttttttt cttcgtctga ggcaaagcat ttcttccata
     7141 ttttgtcagc ctctgctttg cctcttcttc gcctaatcct tctttttcgt cactgtctaa
     7201 aatcttaact acttcttcaa tactaagttc gtgccaattt actcctttct taaccaaggt
     7261 aatacctcct ccaatagccc tcccactctt tgcataagag ttttcctatc ctcttcactc
     7321 tcttttttac ccactatttc ctgtatgaaa ccttccagag cctgttcaaa accaatgtca
     7381 tatccctctt tctgactctt ataccactta taatcaagaa cataggcgaa aatatcagcc
     7441 aagctcttat cggggaaagc ttcttccaac ccccgctcta caatagcctg tactgctgga
     7501 gtgtaaactg acagaaacca attaaggctg gcctctttaa gaggcacctc tcttcccaaa
     7561 aatgcttcca tccttttagc ataatcctgg atataactta aaaaagaatc ataatgtcga
     7621 ggctcactgg catcaatacc tttaagtccg gttaagcttt caaattctgc tcttttcttg
     7681 cgcaaagctc gactctctct caattcggaa ggaaagaatt caatcacttc agcatcaatg
     7741 tattttacgc ccaggtcttt ggctactgct acccgatgat taccatctaa aacataatat
     7801 tcatctccta cctggtaaac aataatagga ggaaggactt ctcctctctc catagctttc
     7861 ctgatgcgat aataacgagc atcagggttc ttgaggcgga aaccgggaag caagtctgcc
     7921 actcttccca cactacccac aattttattt accggtatag tttgcatacc tcgatatact
     7981 cgattagaga gacttaacct tttagaaaca tttccaaaag ctttaacttt ttgttcatct
     8041 ttaccgatga taggcataag aaaaacccct attctcttaa cagcaaaagt tcaggattaa
     8101 aagaagcatc cactcctaaa agagaaaaaa gagtgttcat ttctccagta aatattacca
     8161 cacccaggaa aacgagcaat atcccgctta ttatttctac ccatctccct cttttttgta
     8221 aaccacgaaa aaacgtagtg agacgtcccc agcctattcc caaaaaaata aaaggcaaag
     8281 ccaaacccag agcataaaag aaaaggagca cccctccttg ccaaacctgt cccatactag
     8341 aggcataaag aagaatagac cccaaaatag ggccaacaca aggtgtccac cccaggccaa
     8401 aagatacacc caaaactaag cttcgcactc gagaaagagc catctctttg ggacggaagt
     8461 gtttatcagc atacaaccag cgaattttca aaactcctga aacctgcaaa ccaaaaatca
     8521 caatgatagc gcccgccaag cgattaaacc acacccggta agggagaaga acactcccca
     8581 aaaaagaggc tcctaatccc attaaaatga aaactccact aaaacccgta acaaaaagaa
     8641 gggtatgggt taataattta cttttttcct gttcagtaga ttctactccg ctaatgtagc
     8701 tcacataagc aggagcaata gctaaaacac aaggagaaag aaaagaaaga agaccggcta
     8761 aaaaactgat aaggacgttt agatttacca aattatactc acctcttcac tatactttcc
     8821 tactactatt tccttttctc cctctccgtt cctaaaggaa aagctggata cataaagtta
     8881 tttttaccca tgataagtta acaaaagcgt tttcgattat accaattccc tacattttct
     8941 caattgagat ataattatct ttagttcatt atacgttaac aaaataaaaa aggagagaaa
     9001 aaaattgtac tgggaaaagc aattagaaac tataccccgg gaggaactat ctttactaca
     9061 agcagaaaga ctccaaaaaa ccataatcaa tgcccaaaaa accagctttt atcgccgtct
     9121 ttttgctcgc aataatatag acccggaaaa aatcaagtct cctgaagatt taaaagttat
     9181 tcctcccacc actaaagcag atttacgaga gggtttccct tttgatttct tagctgtacc
     9241 tttagaagaa gtgatacgcc tccattcctc ttctggaacc actggcactc ctacagtaat
     9301 ctattacact gcctcagact tagaccggtg gactaatcag gtagctcgtt ctctatacgc
     9361 tgtaggaatg agaaaaaacg atgtttttca gaatatgatg agttacggac tttttactgg
     9421 aggtttgggt atgcattacg gagcagaaag attaggagct cttaccatcc ctataggtgc
     9481 aggaaacagt aaaagacaaa tctggttttt gaaattcttt aaaagcacag caattcatat
     9541 tattcccagt tacgcccttc tccttgccca attaataaaa gctgaaggct tagaccctca
     9601 tcaggatttc aatattcgca tcatgctttt aggagctgaa cctcacagcg aagagactcg
     9661 actccgtata gaagaaattt atggagctaa ggcttataat tcttatggac tatcagaagt
     9721 caatggacca ggagtagctt ttgaatgtgt cttccaggaa ggaatgcatt tgtgggaaga
     9781 ttattacatt ttagaaatac tcaatcccca gactctctct cctacccctc tcggagaagt
     9841 gggagaaata gttctcacca ccatttaccg ggatgctact cctcttatcc gttaccgaac
     9901 cggcgattta gcttttcttt tagaggagcc gtgtccctgt ggtagaaccc accaacgtat
     9961 ttcccgtatt cagggaagga ccgatgatat gtttatcttc cggggagtaa atatctttcc
    10021 catgcagata gaaaaagtgt taatgagtaa tcccgaagtg ggcaacaact accgcattat
    10081 tttagaaaga gaagattatc gagataaaat gatggtagaa gtggaaataa agccagaact
    10141 tttctctggt gatttgcaaa aattagccag gttgaaagaa agaatccaat ctgagctcca
    10201 ctcagaaatt ctcgtaactc cagaagtggt cttagtagaa ccaggaagta tnnnnnnnnn
    10261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naaaaggagg aaataaaatg gctcgccagc
    10381 tttccatttt tgccgagaat aaaccaggaa gaatcgagaa aattactcgc cttctttccc
    10441 aggaaaacat caacgttcgg gctatcacta tttctagcgc taatgggttt ggagtaatca
    10501 aaattttagc taatgaaccc caaaaagctt tcgatcttct tcgcgataat aattttccgg
    10561 tttatttaca agaagtcatt gccgtagtaa tgaatgatga gccaggaggg ttacatcgag
    10621 tagcacaaac tctatctgca aatcaaatca atattgaaga tgcctatgga tttgtaatca
    10681 aaagtggaga aaaagccgtt ctggtcatcc aggtagaaaa tcaaccccag gctcatagta
    10741 tcctggaaag aaatggcttc cagctcttaa ctgacgaaga aatttaccag ctgtgaaaat
    10801 agaatcacaa gaggagaaat actccttaaa gattaaaata caaaaattag aataaaccat
    10861 atcagattga gctctagaag accagcctat tctatttttc gatccggata attcctggag
    10921 atgggtagcc agacgctgga tcccaggatt actggttatc tatttaatcg acttagatcc
    10981 aaaacaatac tttttcctgg ccacatcagt ttttcgggag atacgaacca gcgagcatat
    11041 tttacatttt tgatttagtt acacccctgg agtaataaaa attagtaaag gtgggaagaa
    11101 aatgaaaaga gtcgtaataa tcgtcatgtt gctggtaaca acaattttag ctggttgcac
    11161 agaggaagtt tcaggaaagc aatcaccaga ggaattaaaa tacgatctta attttctaat
    11221 ccagacactg gaagatgttc atcctgattt atacagatac actcctaaag aggaaatcta
    11281 taagatgaaa caagatattg aggagtactt gagtgagcca ctgaccaggc ttgaatttta
    11341 tagaaaagtt gcacctctgg taaccagaat tggggatgga cacacctata ttacctatcc
    11401 agtggaagag ttaaatgaat atataaatga agggggcgtc ttttcccgct cgaggtcaga
    11461 atcattgatg aaagactatt tgtcgtaatt gattacactg gagagattcc tccgggttca
    11521 gaaatactcc aagtcaacgg actctctacc tcgcagctcg tagaggagct cactaaatac
    11581 gtgagcggag agaaattatc gtaccgaata gagtcagtta ataaaaactt ccgcgcccta
    11641 ttatggtttg tatatggtgg ggaagaattt agcataaaat attcatctcc ttctagtgaa
    11701 actctccaga aacaaataaa gggaatgact ctaaatgagc tcaccagaag agctcaggag
    11761 cttcaaaagg aatccactaa aaaggaggag gtaaaagagg aagatagaac aaaaatccag
    11821 tatccctatt acagttatac ttcagtgtcg gagttaaaaa caggggtaat tgatttcaga
    11881 gcctttcaga atccagaaca gtttaattca tttctggaag aaacattcag gaaaataaag
    11941 gaagaagaaa tcgaatatct tattatagac attcgcaata acagtggagg aaactctact
    12001 ctgggcgatg ctcttctcga ttatctgaca gacaaaccct ggaggcagtt ttcaaagatt
    12061 gaacttaaag taagcaagca aataaaggaa tattaccaag acccccttcc agtattaaaa
    12121 aagatttatc cagaatccta cgaatcggcg tatcaaacat cccgggaatg gtaccaatca
    12181 gtgtatcagg aaattgcttc cctgcctgaa ggcgaaatat ccttctcctc tccagagttt
    12241 acagagcctt ccgaaaaccc ccttcgattt tatgggaagc tatatgtatt aataggctct
    12301 cacacctttt ccagtgccgt caattttgca gccacagtca aagactacgg cattggcaca
    12361 cttattgggg aagaaacagg aggactggct actcaatttg gtgatgttta ccccttctgg
    12421 ctacccaaca ccgacctctc ggcaggggtt tcccacaaga agttctatag acccagcgga
    12481 atagacgacg gaagaggagt tttaccagat atagaagtta ctaccactct tgaagatgtt
    12541 ttaaaaggta aagacccggt gatggaacag gtgctggaga ttatcaaaag caaggtttta
    12601 tagagtttaa attaaagaag agaaaccaca attgacagtg tactggcgcg cctggaggga
    12661 tttgaacccc caacctacag atccgtagtc tgttgctcta tccagttgag ccacaggcgc
    12721 accttaactt gccataatta tatttctact cggctataaa atcaaccttc ctttacttct
    12781 ctacccggta agaacgaccg attattgcta ttgctcccac cactaaaggc aagaaagcca
    12841 cagcgtaaag ataagctcca ttttctttct tcaagaaacg aatagtccgt tcgctctttg
    12901 ggatatgctc cccagtttcc cgggatatag tatatatttg ataatcagct ctggggagat
    12961 cttctcctag ggtaacttta tagctttcaa aggtgggttg ataaccactc ttttttagct
    13021 cctcatcata gggaagtatc tcatacccca gctccggacc ggaaacctgc cttacaaaat
    13081 aaggagcctt gtccaccgtt tgcaatattt cctctctcct tcccagaata atttgagcat
    13141 tattcaccgg gtcaacatgc acccatttcc agctttctaa ccgaccaata ttttgagggt
    13201 cttgaagctt gttataatat agctcattgt actcttcctg ataaaaggga ttgaaataga
    13261 gttcattttt acccacagcg tggataatcc aagaagggtc gtcacaattt tctcttttag
    13321 tccagcggtt tccagggata atttcatatc ccactccttc tcgccggcgg gaggtaaaga
    13381 ctattaaatt tttttcgctc ccttcaaaaa tagttccatc ttccattctc actttaatct
    13441 catagttacc cacaggaagg ttaagaataa aaccttctgc tggctcggtg acatagaatt
    13501 tcacgaattc aggaggctct ggttgttgag gaataggaat atcaagaggc ccggtgtcac
    13561 cagcctctcg gcggcggcgt acctcttcaa agaaattatt cagctgttgc tgataataat
    13621 tcatagcctg gtaatactct tgaagttttt tattgaactc atccactgct gctttatatt
    13681 catcataata tttttgagcc tcatcatcca gataaatcgt agctttttct gaccaatacc
    13741 cttcagggta atagagaaca tacttctgtt tatccagagt gttaattaat tttcctcctt
    13801 gccagatttc cagttttcct ggcacttctt catttaaagt actaaaccca gccatatacc
    13861 ggcgggttat aggccaatag taaacaatag ttttccgggg gcttaccacg ctattaaaat
    13921 tggcaatgaa gtataaagtt ccttcgcttt gaggacagaa actactgcta aagccagacc
    13981 cattgaaaga agtcaaggag taaaccagtt gttcccggaa aagaggtgct tgagcccagg
    14041 aaatacttcc tcccagaacc aagatgccca ctacaataaa gaaaatcgtc tttttattca
    14101 tcaggcctta accccccttc gattcataat aaatatagaa agaaccataa aaatgaggga
    14161 ataaatcaga cagtaggcta tgttagttaa atacaatggc cagctttcca aaaccacagc
    14221 atcaagccct cggtttaaga aagcaaaagg tgatatccag tttataccct gaaagacata
    14281 agagagagtt tgacggagat aaagaagggg gagggatata gttgcttctt ctcctaaacc
    14341 taaaagagca ccataggcaa attgaatggc taaaaaggct cccagaatgg ctaaaagggt
    14401 gattactgag ctgcgaattc gaccggttaa agaagaaatg aaaagtccga agcttaccac
    14461 acaagatatg agaaaaatcc ccatgaacaa ggctttaact aaacctatgg tgaaccccag
    14521 attggtaaag taactcacta agtaaaagta aacagcagtg aaacataaag ctatgatccc
    14581 taaaagtaaa tccttgaagt atttactcca gagaaagttc cccgctgtca ccggcccata
    14641 gaaaagaact tctaaagtcc cctgctccct ttctcgagag atggaaatgg ccgatactat
    14701 tactagatag atagagataa taactaaagt gatatagaga ggttgatgaa ggggataagc
    14761 cgaaaccagg atatcctctt cccggatacc atctataaaa ttctttaaga taaaagaaga
    14821 aaccagaaaa gagacaaaaa tggccgcgta atatccccag ctatataaag tatagagaaa
    14881 atccctttta aaaagagttt tagtcacttt tcctgccatt gtcattcctc ctttttaggt
    14941 aactgtagct ctacatcttc aataacatcc tcaaaatctc cctgaggagt atggagtatc
    15001 aattccatga tgccatattt ttctttctcg ggatcaatct gaattttgtc ccaggtaata
    15061 atacctttgt tacgagaaag ataagcatca ggatcccaga aaagttcaat cggtccttcg
    15121 aaaatcaacc ttccccggct gtagttattt tcatcttctc cctctctaac atagagtcta
    15181 ccttccagtt gaagtggcag agcaggaaaa tccgatattc cagtcccatt agaatcttta
    15241 aagcggattt caatttgaat gcctctatct acggggtcag caaaacatat gataccagct
    15301 aaatcaggaa tttctttccc cttcatggta aagttaaaaa cattaacccc tctttttatc
    15361 tctaactcct cggaaaaagg gtaataatag ggagcttcgg cctggagggt gtattttccg
    15421 ggagaaagat gagtaaattg ataatcttta ctttgaaaca aaatatcaga acgcccatca
    15481 acagtaaggt gtaattttct aaccacgtct tgcgaataat agtcggtcac tttgccctgc
    15541 aaaattccat aatttagccg gacagtaaaa taagaaacca caaataaaac cacaataact
    15601 cctacaatac ttagaaaaat agagagacca gcttttttct tcatgctgct tcctcccccc
    15661 gggctaatag agaaatattt tgctgggtga tggtgataaa ggcttcttct aaactcattt
    15721 ctttagcttt catagaaaga ggaaggtatc cattccgata aagaaattga gataactccg
    15781 gacgataatc ccggtcagtc tttaaattca cttctaactt atgtccttct atattaaatc
    15841 ctttcaaaaa gtccagtttt tctaaagagg aggcgaggtc ccccgcctct ttatctaact
    15901 ctatttccat ctctacagca tcagtcaatc ttctgcgaat gttatccatg gtatcttcag
    15961 ctaaaagcct tcctttattt ataatagcca cttttttgca taatctttct acctcggaga
    16021 gaaggtgaga agaaataaaa agtgttttcc cccgatgatt ttcatcttca attaaatctc
    16081 gaatttgttt gatgctattg gggtctaagt tggccaccgg ctcatctaag ataaggagtt
    16141 ccgggtcatg taaaagagca cgagcaaaag ctaacttttg ctgcatacct ttagaaaaag
    16201 ctcctacttt cttgttctgg tcgttaacta atcccagagc ttccaataat tctccaattc
    16261 ttttggaaac ttgaggtacc tgatagagtt ctgcaaaaaa gagaagatat tcccgagcag
    16321 tcatctcttt ataaaaatat aagctttcag ataccactcc aatttttctt tttacctcta
    16381 ccttaccagg atagaattcc tctcctaaaa gaaaaatttt accactggta ggaggtatta
    16441 accctaaaat catattaata gtggtggtct tccctgctcc attgggacct aaaaaaccat
    16501 atatatctcc cttatctacc tgaagattta agttatccac agccttaaag ccatcaaact
    16561 ccttggttaa gtttatggtt tgaatcatga attttctcta cctcctcttt ttccaaatta
    16621 ctttatttta gcagaaagaa aaaatcaaac caccattttt ggcccttcat tttataagcg
    16681 tatctcaaga gattagcgat agttccttaa aatttttctt ttttataata tctcgtggct
    16741 aatttaagaa tgccagaaga gcaattaccc aaaccaacat atattgttgt cccgggtaaa
    16801 agtcaggatt atagtatccc gcaaaactgg ttaaaatatt aaaactttta aaaagtaaa
//
LOCUS       DAOSVE010000028        15924 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_2258_length_15924_cov_77.676224, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000028 DAOSVE010000000
VERSION     DAOSVE010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 15924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15924
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2258_length_15924_cov_77.676224"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            560..1432
                     /locus_tag="PK016_03790"
     CDS             560..1432
                     /locus_tag="PK016_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HXL02226.1"
                     /translation="MKSMTGFGYAEGAGKLGFYKIRLKTLNHRFLELNCRIAREMNMW
                     EDKITNLVKENISRGKVEVKIEFEPQEGAFSVEANVVLARSYWKALQKIVQELSLLQQ
                     PDLSLLLQVGEIVQIKEESSLWEEEWENFYPLLDRAIQSLVSYREAEGNRLREDLLFW
                     LKELQRLTEEAEKKAPLVETYYRNKLLQRIGEFLPQVKVDETLLAQEVIFYVEKSDIN
                     EEIVRLKAHINRMNKLLSQNKSVGRELEFILQEMNREVNTMGSKSCQVEVSSLVVEMK
                     TIIEKMWEQVRNVE"
     gene            1436..2002
                     /gene="gmk"
                     /locus_tag="PK016_03795"
     CDS             1436..2002
                     /gene="gmk"
                     /locus_tag="PK016_03795"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HXL02227.1"
                     /translation="MSGLLFVLSGPSGVGKSTLRKEVMKRCPALKYSISCTTRAPRRD
                     EKEGVDYHFIDMKTFEEMKEKGELIEWAKVHGNYYGTPRRPVEEWLKEGFDVILEIDV
                     QGAKQVKSNCPDGIFIFVAPPSLKTLEQRLRERNSDPEDEILLRMTNAHLEMQSIREY
                     NYLVINDVLEEAVDKLRSIILAERCRIR"
     gene            2012..2206
                     /gene="rpoZ"
                     /locus_tag="PK016_03800"
     CDS             2012..2206
                     /gene="rpoZ"
                     /locus_tag="PK016_03800"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HXL02228.1"
                     /translation="MLSIDDLVQKVGNIYILAVVIAKRVKQLNEGAVPLIELKEPHSP
                     LFVALEELSTGKISFDFVEE"
     gene            2203..3420
                     /gene="coaBC"
                     /locus_tag="PK016_03805"
     CDS             2203..3420
                     /gene="coaBC"
                     /locus_tag="PK016_03805"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HXL02229.1"
                     /translation="MIIYHPTQFWKGKKIVLGVTGGIAAFKAISLASYLAKAGAQVRT
                     CLTENALSLVGKSSFEAVTGERAYIGLFEGGGEILHINLVRESDAIVIVPATANILGK
                     AACGIGDDLLSTILLVDPGKVILAPAMNVTMWNNPVVQDNLKKVMSRGMKVIYPASGS
                     LACGEEGEGRLPELEELVEGIFYHLYPRTELQGKKVLISAGATREWLDPARFISNPAS
                     GFMGGALASLVRACKGEVKMVVGNVSSRPPSGVEIERVETAQEMKIALEKEWEDSDIL
                     FMNAAVSDFRPLSFSPSKIKKEGKEELTLTLVKNPDILSELAKNKGNRIVVGFCAETD
                     NPQEEAWKKLRKKNADIIVANLITPGESGFEASTNKAWIVDCRGGEKELPLLDKRDLA
                     EEIVSYLFAHFRG"
     gene            3417..5435
                     /locus_tag="PK016_03810"
     CDS             3417..5435
                     /locus_tag="PK016_03810"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02230.1"
                     /translation="MNAHFASLALPLPLFSEFSYLVPPSLEDKIQVGSLVEVEFKNRH
                     LLGWVVALEDKSPPGLEIKEILRLAPVVPLDARKLLWAKTLSQLYLIPRGMMLSFFTP
                     ALLSSFWEAAGKRIFPGTYAEATPESFSVPPQGMNFFVFQEKFGRKKKKWEKLIKNGV
                     LRREEEIFEVRESIHPEKDSGWWSIVTRGEREKFLLSFIEKVQKENRKALFVFPDFRA
                     LDYFTSFIESQFPQLRVMRYDSRLASRRRFLAYKLAEEGDFDLILGTRGSVFLPARDL
                     YYHVIFDPEDKGHYSDRPPFHDTFRLLHEKVKIEGGSLEVVGITPSLSMYHFLSTKEF
                     RKRDFPVFPRISPPPVNLVSYEKKWDSSLTFSVRKAILQVVSKKGTVLVWVQKRGYTP
                     ALGCRDCGFYYICPHCGVAFRYYKETQTLICPLCGKKGKIDLFCPRCGGVWLEGWGEG
                     VEKVGEEMKKVFSHQQVAVIDSEKEEEMGESLSFSILVGTSLLLREEILQKASLLVIW
                     SLEDWLYLPDMNAREEFYLNMQRIRTFLGLNSPFPPRIFIQGRKGMEKEIGQFLQPPH
                     DFYPPLLEKRKILGYPPYGVLAHVIKRYRNREKDGLVLKKLRGCLEEKGIEAGGPFPA
                     QGRKWGEESEEITIHYSREKAGEVFDVVSQFFSQEGKRKGEWQVEVEF"
     gene            5453..5956
                     /gene="def"
                     /locus_tag="PK016_03815"
     CDS             5453..5956
                     /gene="def"
                     /locus_tag="PK016_03815"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HXL02231.1"
                     /translation="MPIREIKIYGSPVLREKAQPIENIDQKVWNLAQDMKEIMHLAKG
                     VGLAGNQVGVLWRIITFTNPEEQKDQALINPQIIHLSEDKEKDEEGCLSFPDIYDQVE
                     RAKKVVVKGWDLQGKEVTIEGEGLLARILQHEIDHLDGILFVDRLSPTRRLLLHNKLR
                     KISVARE"
     gene            5960..6889
                     /gene="fmt"
                     /locus_tag="PK016_03820"
     CDS             5960..6889
                     /gene="fmt"
                     /locus_tag="PK016_03820"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HXL02232.1"
                     /translation="MKIVFMGTSSFAVLVLEELLKEKKNNNIVGVVTKRDRPAGRGRK
                     LRPSPVKEKAEEEGLTIWQPERVNAPEFLELLRGVSPDIIIVAAYGQILRRDLLSIPP
                     KGVINVHASLLPRFRGPDPIRSALLQGEKETGVTIMLIDEGVDTGPVLAQKKVEIEKE
                     DNYQSLTEKLGKEGGKLLVETLPRWVRGEIAPRPQEGEVSYAPILKKEKARINWEDSA
                     FNIVNQIRAFSPYPGAYTYFRDRRIKILRASLKEGISAPPGTVVEIHPQGFVIATGEG
                     GVVIEEVQPENRPAMTSREFCCGYPLREGDQLL"
     gene            6902..7579
                     /locus_tag="PK016_03825"
     CDS             6902..7579
                     /locus_tag="PK016_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006905031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HXL02233.1"
                     /translation="MFFFDYTFILLIPALILAFYAQAKVSSTYSKLSKKRASAGLSGR
                     EVAERLVDSLGLRVRVEEIPGALTDHYDPTREVLRLSQAVANGSSIADYSIVAHEVGH
                     VLQKREQYPAFALRSTLVPVASFGSQLALPLFLIGLIFYLPALMDLGIIFFSLAVLFQ
                     LVTLPVEYNASSRAYTVLDRAGIIAVEEKPYIKQMLNAAALTYVAATAMAALQLLRLV
                     LLRGSRN"
     gene            7576..8853
                     /locus_tag="PK016_03830"
     CDS             7576..8853
                     /locus_tag="PK016_03830"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HXL02234.1"
                     /translation="MSDGRRKDALQILNRLYSSRVSFLDAEYNRWIKKGDYTEKDRSW
                     LVSLLRNLVKHQAFIDKVLKEYLPRWEKLPLATQNLLRMGVAELLFHSSPSFAIVNET
                     VELAKATGAEFAPLVNAVLRKVARKRDYYYAILEEEGIELPPFLEEEWRNWIGEEELK
                     LLKKSLSLPPLLYVRVNTLKIGEDELMERWREKGIESSKTVLPGALKVDVDYSTLLAL
                     PEYQEGLFYPQDLGSQLVVKTINPFIRGRVIDVCAGVGGKTFTMAQYLSGGEILAWDK
                     KESKVSTLSKFISLFNLSKVKPMVVDALHPPREFFSTADLVVVDAPCSGWGTLRRNPE
                     ILLKNPDLEDLSRQQLSLLLSASSLVKRGGVMVYCVCSLTQEETKEVVKKWETQEGKG
                     WEKVDLGAREKEMVIWPHQLNSDGFFVTVWRKK"
     gene            8850..9899
                     /locus_tag="PK016_03835"
     CDS             8850..9899
                     /locus_tag="PK016_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HXL02235.1"
                     /translation="MREEKKETWSSFILRVFLYALLFGGGLALSVYLGLILFQYYLTS
                     DILSLPDFRNQSLVEAINQMSRLGLQAEIKRIENNPEFPSNWVIQQDPPPGVEVKKNH
                     RVKLVINGGEGIAEGGSISGGESPSFSASAPTSVSVPDVRQMSLEEAQSLIESSSLKL
                     ERVAEVTHDTIPAGYVVSQNPPPESRVAKGSVMSLLVSKGKSETEVLNLVVVPDLVGL
                     RLEEAKRLLAQSNLTVGNVEEVAAEGRSAGVVVGQSPAPGEEVEPGEKIHLQVTKPEE
                     EASGLKELSLRFVLPSSRNPIEVKVVVTDELGERVVYEKEHQGEEMVELNTSTKGEGR
                     VVIYLNGYYYWDKTL"
     gene            9921..10586
                     /gene="rpe"
                     /locus_tag="PK016_03840"
     CDS             9921..10586
                     /gene="rpe"
                     /locus_tag="PK016_03840"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HXL02236.1"
                     /translation="MARLAPSILSCDFSQLKEELLAAQEGGAEIIHVDVMDGHFVPNL
                     TFGLPILKAVRKILPRVTIDVHLMTDNPRLYIADFIREGANMVSFHLEASSDYSSIVD
                     EITKRGAKPGVALSPGTPWQCLSPVLDKLYYVHLMTVHPGFGGQKFIPGMEDKIASLR
                     REIRRRGLSTEIEIDGGVNDQNIEYLAFCGASIIAAGSLVFAHPENIAARVKMLSEKI
                     KPY"
     gene            11243..11590
                     /locus_tag="PK016_03845"
     CDS             11243..11590
                     /locus_tag="PK016_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02237.1"
                     /translation="MMFSNPSLASVLGAAFPAQFVLAPLFMGGEIEEGFTWKEKDEEG
                     KETEISVISTKTRFGKEAFWVEIKTNGMVVMQILAEKETYFLFILDIKDKELLEPEQQ
                     RIYLEVEEVTWRE"
     gene            11864..12139
                     /locus_tag="PK016_03850"
     CDS             11864..12139
                     /locus_tag="PK016_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02238.1"
                     /translation="MDIKNYTREMADRRFAALETLKTITEWLNATKPTSFLEKGAPVG
                     DDQIREIWRRWVDAYSDLFEKTKVIPDLLIEDVRKLYASEKGTSEGQ"
     gene            12105..12422
                     /locus_tag="PK016_03855"
     CDS             12105..12422
                     /locus_tag="PK016_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02239.1"
                     /translation="MPPKREHLRASRILLGTQTPVVHELLELAVRKQGMEHGYLVSNS
                     DYLGMIGNLLGQEAKREAVLHILQDWGIVTQEDVDHFSTFIPSRRKQTMSSNSGKKKK
                     SFQ"
     gene            complement(12848..14563)
                     /locus_tag="PK016_03860"
     CDS             complement(12848..14563)
                     /locus_tag="PK016_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HXL02240.1"
                     /translation="MEDYKNFRVALYCPAPFLKDATFNELEEGFNFFKKHLKISKVYL
                     ETHRGNSDVEKEKILKVKEFFEKEGIKTSGGITTTILLENDEFKDLRIFNTFCYTNPE
                     HLKKLVEIVEFTASIFDEIILDDFYFTSCRCPLCIEKKGDLSWEEFRLNLMASISEEY
                     VVKPAKRVNPEVKMIIKYPNWIESYQETGYNPRAQAEIFDYVYTGTETRNPLFTQQHL
                     PRYLSYSLMRFFDEVKPGKNLGGWFDSFDCIYNIGYYLEQAYLTLFSRTEEITLFDFS
                     SLRNSVFIPPLGHQLGLLDSILGELGNPSGIPVYYPFNSYGEDHLYDYLGMAGIPLYP
                     TPYFPEDSDLIFITANSVKDKDILYKLKKCLLTGKNIVMTSGFLKAVQGKGGEDFTSL
                     RVTDNKVISNFFGVKTQNSGFSQYVHSQKKIVFPVLNYRNNATWPEIVSIDGENNFPI
                     LMNDSYGRGRIFTLTIPDNFSDVYYLPREVLTEIRKIFMMGNPFYIECEGKVSLFTYD
                     NDAFVIESFLPHRTLVNIYIKDKNRRLIDAKEGFEYQPIFSSEKESLYKINLEPSSFG
                     VFKMV"
     gene            14956..15468
                     /locus_tag="PK016_03865"
     CDS             14956..15468
                     /locus_tag="PK016_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HXL02241.1"
                     /translation="MKEENLAGMLESVLAGRVKEYKLVDPKTVVVAEWVRFKCQFGCD
                     GYAQSFTCPPYSPEPSRTKRMLEEYELAAILWKPDSYENLRKITTDLERTLFLSGYYK
                     ALAIPSGPCELCNPCPLEYPCQHPEYARPSMEACGIDVYATVRQHGFPIEVVRSRNCQ
                     PNYYALVLIE"
     gene            15488..>15924
                     /locus_tag="PK016_03870"
     CDS             15488..>15924
                     /locus_tag="PK016_03870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXL02242.1"
                     /translation="MATLFHSEASFQFSTNKKKWLIVLLFIGGIFVRLFLLGVIPPGL
                     NQDEASIGYDAWALLHYGIDRNACHNPVHLISWGSGQNALYAYLSMPFILLFDLNPFS
                     VRILNALLGCLSSVIFYLLVKKIWDEDTGIIALFLLVISPWHI"
BASE COUNT         4864 a         2886 c         3571 g         4603 t
ORIGIN      
        1 cattacccat aagttttttc tggacaccat agaaggtttt caaaacagat aaatggacag
       61 aaaggaataa ctactaacta cctgggagat taaactatat cctctaaacc ctgtaaaccc
      121 aatcatataa gttcaagaga gatatcatag ttaaccaaaa atcttactgc cattctccgt
      181 catcgcgagc ccggacatcc tgcggcacaa gattaaaagg caaaagcgat gaaaaggact
      241 aaaactacgg aggaagaaaa agccttcctc cgggcgtagc gatcccgagt ttttgacttc
      301 cggtgtaatt tactttttaa aagttttaat attttaacca gttttgcggg atactataat
      361 agcattcctc ataaatttcc tgcatattta ccattgtaat aaaaatgata cacctctcga
      421 aaatcactcc cgggtaaggt agctttttca gagacctttc tttctccgat gaataataat
      481 tgacagggtt aaacttatta aataatatag tattatattt caagctctac tacctgattt
      541 tagaaggagc ataaatttaa tgaaaagtat gaccggtttt ggctatgcag aaggagcagg
      601 aaaattaggc ttctataaga ttcgtttgaa gactttaaac caccgtttcc tggaacttaa
      661 ttgtcgtatt gcccgggaga tgaatatgtg ggaagataaa ataacaaatc tggtgaagga
      721 aaatatttcc cggggaaaag tggaagttaa aatagagttt gaacctcaag aaggggcttt
      781 ttcagtagaa gccaacgtag ttttagcccg ttcttactgg aaagctcttc agaagattgt
      841 gcaggaatta agcctgcttc agcaacctga cctttctctt cttcttcagg taggagaaat
      901 tgtgcagatt aaagaagaaa gctctttgtg ggaagaagaa tgggaaaact tttatcctct
      961 tcttgaccgg gctattcaat ctcttgtttc ttatcgagaa gcggaaggaa atcgtctccg
     1021 ggaagatctc ctgttctggt taaaggagct acaaaggttg acagaggaag cagaaaagaa
     1081 agccccttta gttgaaactt actatcgcaa caaattactt cagcggatag gagaattttt
     1141 gcctcaggta aaagttgatg aaacgcttct agctcaggaa gtaatttttt atgtagaaaa
     1201 aagcgatatc aacgaagaga tagtgaggtt gaaagctcac attaaccgca tgaataaact
     1261 cctttctcaa aataaatcag tgggtaggga gttagagttc attcttcagg agatgaatcg
     1321 agaagttaat actatgggct caaaatcctg ccaggtagaa gtctcatctt tagtagtaga
     1381 aatgaagacc ataatagaga aaatgtggga gcaggttcgt aatgtggagt aaatgatgag
     1441 tggattgctc tttgtcctgt ccgggccctc gggagtaggt aaaagtaccc tgcgtaaaga
     1501 agtgatgaag agatgtccag ctttgaaata ttctatttcc tgtaccaccc gtgctcctcg
     1561 ccgggatgaa aaagaaggag tggattatca ttttatagat atgaaaactt ttgaggaaat
     1621 gaaagaaaag ggggagctta ttgagtgggc aaaagtccac ggtaactact atggtactcc
     1681 tcgccgtccg gtagaagaat ggttaaagga aggatttgat gtgattttag agattgatgt
     1741 ccaaggggca aaacaggtga aaagtaattg tccggacggg attttcattt ttgtcgctcc
     1801 tccttctctt aaaactttag aacagaggct cagggagagg aattctgacc cggaagacga
     1861 aattctattg cgtatgacca atgcccatct tgagatgcaa tccattaggg agtacaatta
     1921 tttagtgatt aatgatgtcc tagaagaagc agtggataag cttcgttcta taattttggc
     1981 tgagagatgc cggataaggt gaggtgagag tatgctctcc attgatgatc tggttcaaaa
     2041 agtaggtaat atatatattc ttgctgtagt gatagctaaa agagttaaac aattaaacga
     2101 aggtgctgtg cctcttatag aattgaaaga acctcatagt cctttatttg ttgctcttga
     2161 agaattaagt accgggaaaa tcagctttga ttttgtagaa gaatgattat ttaccatcct
     2221 actcaattct ggaaaggtaa aaaaattgtt ctgggagtga cgggagggat agcggctttt
     2281 aaggctataa gcctggccag ctatctcgcc aaagccggtg ctcaggtaag aacttgtttg
     2341 actgaaaatg ctctatctct cgtaggtaaa agttcttttg aagcggtaac aggagagaga
     2401 gcttatattg gcctttttga agggggagga gaaatcctcc atattaattt agtaagagaa
     2461 agtgatgcca tagttattgt tccagccact gctaatatct taggaaaagc agcctgtggt
     2521 attggcgacg acctcttgag caccattctt ttagtagacc cgggcaaagt tattttagct
     2581 ccagctatga atgttaccat gtggaataac ccggtggtgc aggataatct gaaaaaagtt
     2641 atgagccggg gaatgaaagt gatttatcct gcttcaggaa gtctggcctg tggagaagaa
     2701 ggagaaggaa ggttaccgga actcgaagaa ttagtagagg gaatttttta tcacctttac
     2761 ccccgcaccg aactccaggg taaaaaagtc ttaatcagtg ctggtgccac ccgggaatgg
     2821 ttagacccgg cccggtttat ttctaacccg gccagtggct ttatgggagg agccttagcg
     2881 tcacttgtgc gagcctgcaa gggagaagta aaaatggtag tgggcaacgt ttcttctcgt
     2941 cctccttccg gagtggagat agaaagagtg gaaactgcgc aggagatgaa aatcgcctta
     3001 gaaaaagagt gggaagatag tgatatttta tttatgaatg ctgcagtttc tgactttcgc
     3061 cctctttcct tttctccttc taaaattaag aaagagggaa aagaagagtt aactcttacg
     3121 ctggttaaaa atcctgatat tctatctgag ttagctaaaa ataaaggaaa tagaatagta
     3181 gtgggtttct gtgctgaaac ggataaccct caagaagaag cctggaagaa attgcggaaa
     3241 aagaatgcag acataatagt ggctaacctc atcacccccg gggaaagcgg gtttgaggct
     3301 tctactaata aggcctggat agtagactgc cgggggggag aaaaagaatt acctctttta
     3361 gataaaagag atttagcaga ggaaatagtt tcttatcttt ttgctcactt ccggggatga
     3421 atgcacattt tgcttctcta gctttgcctc ttcctctttt ttctgagttt tcttatttag
     3481 ttcctccttc tcttgaagat aaaattcaag taggtagttt agtagaagta gagtttaaaa
     3541 atcgccacct tttaggatgg gtagtagctt tagaggacaa atctcctccc gggttagaaa
     3601 tcaaagaaat attacgattg gctcctgttg ttcctttaga tgcaaggaaa ctactgtggg
     3661 ctaaaactct ctctcagctt tatttaatcc cccggggaat gatgctttct ttttttaccc
     3721 cggcgctttt gtcttccttc tgggaggcag cgggtaaaag aattttccct ggcacatacg
     3781 ctgaggcaac gccagagagt tttagcgttc ctcctcaggg gatgaatttt tttgttttcc
     3841 aggaaaaatt tgggcgaaaa aagaaaaagt gggagaagtt gattaaaaat ggagttctgc
     3901 gccgggaaga agaaattttt gaggtaagag aaagcattca cccggaaaaa gattccggct
     3961 ggtggtctat tgtcacccgg ggagagcgag aaaaatttct tctatccttt atagaaaaag
     4021 tgcaaaaaga gaaccggaaa gctcttttcg tgtttcctga cttcagagct cttgattatt
     4081 ttacttcttt tattgaaagc cagtttcctc aattgagagt gatgagatac gatagccggc
     4141 tggcctctcg ccggaggttt ctggcttata agttagctga agagggagat tttgatttaa
     4201 ttctgggcac tcgaggaagc gtatttttgc ccgctcgaga cctttactat catgtgattt
     4261 ttgacccgga agataaaggg cattattctg accgccctcc tttccatgac acttttcgac
     4321 ttctccatga gaaagttaaa atagaaggag ggagcttaga agtagtaggt atcacgcctt
     4381 ctctttctat gtatcatttt ctaagcacca aagagttccg aaagagagat tttcctgtct
     4441 ttccccggat ttctccccct ccggtcaatt tggtttctta tgaaaaaaag tgggattctt
     4501 ctttaacttt ttcagtgcga aaagctattc tccaggtggt gagtaaaaaa ggtacggttt
     4561 tagtctgggt acagaaaaga ggttatactc cagctctagg ctgtcgagat tgtggttttt
     4621 attatatttg tcctcactgt ggagtggcat ttcgctatta taaagaaacc caaaccctga
     4681 tatgtcctct gtgtggtaaa aaggggaaaa tagatttatt ctgcccccgc tgcggaggag
     4741 tgtggttaga aggatgggga gaaggagtag aaaaagtagg agaagagatg aaaaaagttt
     4801 tttctcatca gcaagtggcg gttattgatt cagaaaaaga ggaggaaatg ggagagtctc
     4861 tttctttttc cattttagtg ggcacttctt tgcttctgcg agaagaaata ttacagaaag
     4921 cctctctcct tgttatctgg tcactggagg actggctgta tcttcctgat atgaatgcca
     4981 gagaagaatt ttatttgaat atgcagcgaa tacgaacttt cctgggtctt aactctcctt
     5041 tccctccccg gatttttatt cagggaagga aaggaatgga aaaagagata ggtcaattct
     5101 tgcaacctcc ccatgatttt tatcctcctc ttttagagaa aagaaagatt ttaggttacc
     5161 ccccctatgg ggtgttagct cacgtaataa agaggtacag gaatagagag aaagatggat
     5221 tggttttaaa gaaattaagg ggttgtttag aggagaaagg aatagaggcg ggcggccctt
     5281 tcccggccca ggggagaaag tggggagagg aaagcgaaga aataaccatc cattattccc
     5341 gggagaaagc aggagaagtg tttgatgtgg tttctcagtt tttttctcaa gaaggaaaaa
     5401 gaaagggaga atggcaggta gaagtagaat tttaaaaaag gagtgaaatt agttgccaat
     5461 cagggaaata aaaatttatg gcagtcctgt attaagagaa aaagcacagc ctatagaaaa
     5521 tatagaccag aaggtgtgga atctggctca ggatatgaaa gagattatgc atcttgccaa
     5581 aggagtgggg ttagcaggga atcaagtagg ggttttgtgg cgtatcataa cttttactaa
     5641 ccctgaagag caaaaagacc aggctctcat taaccctcag ataatccact tgagtgagga
     5701 caaagaaaag gatgaagagg gatgtttgag ttttcccgat atctatgacc aggtggaaag
     5761 agccaaaaaa gtggtagtaa aaggttggga tttacaaggg aaagaagtca ccatagaagg
     5821 ggaaggactc ttagctcgta ttttgcaaca cgaaattgac catctagatg gaatattatt
     5881 tgtagaccgc ttaagcccta cccgtcgttt gctccttcat aataagttga gaaaaatttc
     5941 tgtagcgagg gaatgaaatt tgaagatagt ttttatggga acttcctctt ttgctgtttt
     6001 agttttagag gaactcctaa aggagaaaaa aaataataat atagtaggag tggtgaccaa
     6061 acgggaccgt ccggcgggaa gaggaagaaa attacgtccc tctccagtta aagaaaaggc
     6121 cgaggaggaa ggactgacca tctggcaacc agagcgagtg aatgctccag agtttttaga
     6181 gcttttgcgg ggcgtttctc cagatattat aatagtggct gcctatgggc agattttacg
     6241 ccgggattta ttgtccatcc ctcccaaggg ggtaataaat gttcatgctt ctcttcttcc
     6301 tcgctttaga gggccagacc ctattcgctc tgctctcctg cagggagaaa aggagaccgg
     6361 ggttaccatt atgctcatag atgaaggggt agataccggt cctgtattgg ctcagaagaa
     6421 agtggaaata gagaaagagg acaattacca atctttaacc gagaagctgg gaaaagaagg
     6481 ggggaaactt ttagtagaaa ctttacctcg ctgggtaaga ggagagattg cccctcgtcc
     6541 tcaagaggga gaagtttctt acgctccgat attgaaaaag gaaaaagcca gaataaactg
     6601 ggaagatagt gcctttaata tcgtaaatca gatcagagct ttttctcctt acccgggggc
     6661 ttatacttat tttcgggata ggaggattaa aattttaagg gcctccctaa aagaggggat
     6721 ttctgctcct cccgggacag tggtagaaat tcatccgcag ggatttgtaa ttgctacagg
     6781 tgaaggagga gtagtgatag aggaagtgca gcccgagaac cggccagcta tgacttctcg
     6841 agagttttgt tgtggatatc ctttaagaga aggagaccaa ttactatagg aggtgaaggg
     6901 aatgtttttc tttgattata cgtttattct tttaattcct gcgctcattc ttgcttttta
     6961 cgctcaggct aaagtaagca gtacttattc taagctatct aaaaagagag ccagcgcagg
     7021 attaagtggt agagaagtag cggaaaggtt agtagatagt ttagggctaa gggtaagagt
     7081 ggaggaaata ccaggagcac taactgacca ttatgacccc actcgagaag tgttgcgtct
     7141 ttcccaggca gtggctaatg gttcatctat cgctgattat agtatcgtag ctcatgaggt
     7201 ggggcacgtt ctgcaaaaaa gagagcaata tcctgctttt gctttgcgct ctacacttgt
     7261 tccggtagca agctttggct cacagttagc tttgcctctc tttttgatcg ggttgatttt
     7321 ttaccttcct gctcttatgg acctgggtat catctttttc agtctggcag tcctcttcca
     7381 gctggttact ctaccggtgg aatataatgc cagttccaga gcatatactg ttttagatag
     7441 agcagggata atagcggtag aagaaaaacc ttatattaaa cagatgttaa acgctgctgc
     7501 tcttacttat gtggctgcta ctgcgatggc tgcactgcaa ctcttgcgat tggtgctcct
     7561 gcgggggagc aggaattgag tgatggtcga aggaaagatg ctttacagat cctcaaccgc
     7621 ctttacagta gccgggtttc ttttttagac gctgaatata accggtggat taaaaaaggg
     7681 gattatactg aaaaggaccg gagctggtta gtcagtttat tgagaaactt agtaaaacac
     7741 caagcattta tagataaagt gcttaaagag tacttaccta gatgggaaaa gctaccgctc
     7801 gctacccaga atcttttacg gatgggggta gcggagcttt tgtttcactc ttctccttct
     7861 tttgccatag tgaacgaaac ggtggaatta gcgaaagcga caggagcaga atttgctccc
     7921 ctggttaatg ctgttctgcg aaaagtagcg agaaaaagag actattatta tgctattctt
     7981 gaggaggaag gaatagagct tcctcctttt ttagaagaag aatggaggaa ctggatagga
     8041 gaagaggagt taaaattgct taagaaatct ttgtctttgc ctcctctttt atacgtgcgt
     8101 gtgaataccc tgaaaatcgg agaagatgaa ttaatggaga gatggagaga aaaagggata
     8161 gaaagcagta aaaccgtgct tccgggagct ttgaaagtgg atgttgatta ctctactctt
     8221 ctagcccttc ctgaatatca ggaagggttg ttttatcctc aggatttagg gtctcaactg
     8281 gtggtcaaaa ccatcaatcc ttttatccgg ggaagggtaa tagatgtctg cgctggagta
     8341 ggaggaaaga cttttaccat ggctcaatat ttatctgggg gagaaattct ggcctgggat
     8401 aagaaagaga gcaaagttag caccctctct aaatttattt ctctgtttaa tcttagcaaa
     8461 gttaagccca tggtggtgga tgctcttcat cctccccggg aatttttttc tactgctgat
     8521 ttagtggtag tggatgctcc ttgttcaggt tggggaactc tccgtcggaa ccccgaaata
     8581 ttattgaaaa acccggactt agaagattta tctcgtcagc aactttctct tcttctttcc
     8641 gcttcttctt tggtaaaaag aggaggagta atggtatact gtgtttgcag ccttactcaa
     8701 gaggaaacca aagaagtggt aaagaaatgg gaaacacagg aaggaaaagg gtgggaaaaa
     8761 gtggatttag gagcgagaga aaaggaaatg gtaatctggc ctcatcaact taatagcgat
     8821 ggattttttg ttactgtctg gaggaaaaaa tgagagagga aaaaaaggag acctggagtt
     8881 cttttatact tcgagttttt ctttatgccc ttctttttgg tggaggttta gcactcagtg
     8941 tttatttagg cttgattctt tttcaatatt atcttacaag cgacattcta tctttaccgg
     9001 attttcgcaa ccagagccta gtagaagcta tcaatcagat gtctcgcctg ggtttacagg
     9061 cagagataaa gagaatagaa aataacccgg aattcccttc caactgggtt attcaacaag
     9121 atcctcctcc gggagtagaa gtaaagaaaa atcatcgagt aaaactggta atcaatggag
     9181 gagaaggaat agcggaagga ggaagtatat ccggcggtga atctccttct ttttctgcct
     9241 ctgcccccac atctgtttct gttcccgatg ttcggcagat gagcctggaa gaagctcaaa
     9301 gtcttattga aagtagcagc ctgaagctgg agagagttgc agaagtaaca catgacacca
     9361 ttcctgcggg ttatgtagtt tcccagaacc ctcctccaga gagcagagtg gcaaaaggta
     9421 gcgtgatgag ccttctcgta agtaagggaa aaagtgaaac tgaagtgctc aatttagttg
     9481 tggttcctga tttggtggga ctgcgcttag aggaagccaa aagattactg gctcagagta
     9541 acctcactgt gggaaatgtg gaggaagtag cggcagaagg aagaagtgct ggggtagtgg
     9601 taggacaaag tcccgctccc ggagaagaag ttgaaccagg agagaaaatt catcttcagg
     9661 taactaaacc tgaagaagag gcatcaggct tgaaagagtt aagtttaagg ttcgttttac
     9721 ccagttctcg aaatcccatc gaggtaaaag tggtagtcac tgatgagcta ggagagcgag
     9781 tagtttatga aaaagaacat caaggagaag aaatggtgga gctgaacact tccacaaaag
     9841 gtgagggtag agtggttatt tatcttaatg gttactatta ctgggataaa actctttaat
     9901 tgacgtaagg agggaaatta atggccagac tcgctccttc tattctatct tgtgatttct
     9961 ctcagcttaa agaagagtta ttagccgccc aggagggagg agcagaaatc atccatgtag
    10021 acgtaatgga tgggcacttt gttcccaacc ttacttttgg acttcctatt ttaaaagcag
    10081 tgaggaagat tttgccccgg gtgactatag atgtccatct tatgactgat aacccccgtc
    10141 tttacatcgc tgactttatt cgagaaggag caaacatggt tagcttccac ttagaagcgt
    10201 catctgacta tagctcaata gtagatgaaa ttacaaagcg gggagctaag cctggagtgg
    10261 cactgtctcc tggaacaccc tggcaatgtt taagtccggt tctggataaa ctttattatg
    10321 tacatttgat gactgttcat cctggttttg gagggcagaa gttcattccc ggtatggaag
    10381 ataaaatcgc ttctcttcgc cgggagataa gaagaagagg cctgtctacc gaaattgaaa
    10441 ttgacggagg agtaaacgac cagaatattg aatatctggc tttttgcggt gcatccatca
    10501 tagcagcagg ttctttagtt tttgcccatc cagaaaacat tgccgctcga gttaagatgc
    10561 tctcagaaaa gataaaacca tactgattat aaagggagag accccctcat aaccccgaac
    10621 tataaagggg ctctgctcct ttataatccc caaaaacaaa aacctaaaag aacaaagacc
    10681 actgaggaaa aaactccctc agtataaggg ggaatgtttc ccccttatca actccttcct
    10741 tttcaccctc ccctcatcca tccttctaag agagggagaa ggagaagact tccacgagtt
    10801 tcacaaagag caacctgcag tgcaaaataa gaaaaattaa atatcttaaa aacaggcatt
    10861 tttatgggaa aagttgcaag ttggataact ctttcaattc tatatctcta tcttttacag
    10921 tgtgctacaa taaaaagact aaaatttgaa caggggggga caaatttgaa aataaaaagc
    10981 cggcctttag aagttacttt tctgctggta gtttttgctt tacttctttc gtcctgtata
    11041 gatttaggaa atgtggaaaa agtaaaaatt caatatcctt gtagaggaag aaaaaggttc
    11101 caaagaggga tatctttttc tccaagttat taccaaagga gaagataaat ttcagggaac
    11161 cataaacttt agcctgggag acgagaaatt ttctaatact ttcactaccg aagaagatga
    11221 ccttcaaaat gttctcacct caatgatgtt ttccaatcct tccttagcca gtgttctagg
    11281 tgctgctttt cctgcccaat ttgtgcttgc ccctcttttt atgggtggag aaatagagga
    11341 agggtttacg tggaaagaaa aagatgaaga aggtaaagaa acagaaatca gcgttatttc
    11401 aaccaagacc cgttttggta aagaagcctt ctgggtggag attaaaacca atggaatggt
    11461 agttatgcaa attttagccg aaaaagaaac ctatttcctc tttatattag atatcaaaga
    11521 taaagaatta ctcgaacctg aacaacaaag aatatacctc gaggtagagg aagttacttg
    11581 gcgagaataa attttaaacc agttttaaaa cttagtagag gttatttact ttcaccattg
    11641 caaagattac catctataat ctaaagatca caactttatg atgatacaag ggtagttacc
    11701 gggagggtat attaactctc cgggggaaaa gctattttgg tagacagctt taacaaagca
    11761 gtattatacg aataaattcc tgagaacatg taaataatct tcttgataga aagagacaga
    11821 aaatgtagaa taaagaaaca aaagatgtaa aggaggaatt attatggata ttaaaaatta
    11881 cactcgagaa atggctgacc ggcggtttgc tgccctggaa actttaaaaa ccattaccga
    11941 atggcttaat gccaccaaac ccacatcttt tttagaaaaa ggggctccag ttggtgatga
    12001 tcagataaga gagatatggc ggaggtgggt ggatgcttat agtgatttat ttgaaaaaac
    12061 taaagtcata cctgatttac tgattgagga tgtgagaaaa ctttatgcct ccgaaaaggg
    12121 aacatctgag ggccagtaga attttactgg gaactcagac tccggtagta catgagcttt
    12181 tagagctggc agtaagaaaa caggggatgg aacatggtta tctggtttct aattcagact
    12241 acctgggtat gataggcaat ctcctcgggc aggaagcaaa aagagaggct gttttacata
    12301 tcttacaaga ttggggaata gttacccagg aagatgttga ccacttttct accttcattc
    12361 cctcccggag aaagcagacc atgtcttcaa actccgggaa aaagaaaaaa agcttccagt
    12421 agctctccaa actggcttta tcgcctggcc ttgagagaaa actttagaat atattacatt
    12481 ttgaccccag gtgtagaaaa gtttttagaa cctcttgagg agctgaaaga agggtaaaaa
    12541 gagagccgaa gttggtgttg agctttagaa accagcttct ctacttccct taagagttat
    12601 ctcccttatc gagcgaggta taaatttagt ttaagatttg ccatgcatca caggtttttc
    12661 agtaatagga ctgtatctgg actatcaagt ccagataccg agagcgttaa aatacgccga
    12721 cttctttaat ttctattgaa aaagcagtaa ttttgctttg attttaacta aggcacctca
    12781 atcgttataa tttaacaatt gcccagatga agagagccct gatgcaaggg ctctctaaag
    12841 tccagactta caccattttg aaaactccaa agctagaagg ttccaggttt attttatata
    12901 aactttcttt ttctgaagag aatataggtt ggtattcaaa tccttctttt gcatcaatta
    12961 atcttctatt tttatctttg atatagatat ttaccagagt tcggtgaggc aagaaagact
    13021 caataacaaa agcatcgtta tcatatgtaa acagactgac tttcccctca cattctatat
    13081 aaaaagggtt tcccatcata aatatttttc taatctcagt aagaacttcc cggggaagat
    13141 aatacacatc agaaaaatta tcaggtatgg tgagagtaaa aattctccct ctcccataac
    13201 tgtcgttcat taagatagga aaattgtttt ctccatcaat acttactatc tctggccatg
    13261 tggcattatt tctataattt aatacaggaa agacaatctt tttctggcta tgaacatatt
    13321 gagaaaaacc tgaattttga gtctttactc caaagaaatt agaaattact ttgttatcgg
    13381 tgacccttaa agaagtaaaa tcctctcctc cttttccctg cacggccttc aaaaaccccg
    13441 aggtcataac gatatttttg cctgttaata aacatttttt taacttatat aaaatatctt
    13501 tatccttaac tgaattagct gtaataaata ttagatcgct atcttcagga aaataaggag
    13561 taggataaag gggaatacct gccatgccta aatagtcata taagtgatcc tctccataag
    13621 agttaaaagg gtaataaacc ggaatccccg aagggtttcc cagctctccc agtattgaat
    13681 ccagtaaacc taactgatgc cctaagggag gaataaatac agagtttctc aaggaactga
    13741 aatcaaaaag agtaatttct tcagttctgg aaaataatgt taaatacgct tgttcaagat
    13801 aatagcctat gttgtatata cagtcaaaag agtcaaacca gcctcccaga tttttacccg
    13861 gctttacctc atcgaaaaat cgcatcagag aatagcttaa atatctcggt aaatgttgtt
    13921 gagtgaaaag aggattacga gtttcagtcc ctgtgtaaac atagtcaaaa atttctgcct
    13981 gggctctagg attataccct gtttcttgat aggactctat ccagttggga tatttaataa
    14041 tcattttcac ttccgggttt actcttttcg ccggcttaac cacatattct tcagaaattg
    14101 aggccattaa gttaagcctg aattcttccc agctcaaatc cccctttttt tctatacaca
    14161 aaggacaacg acaggaagta aaataaaagt catctaatat aatctcatca aatatagaag
    14221 cagtaaattc tacaatttcc actaactttt ttaaatgttc cgggttagtg taacaaaagg
    14281 tgttaaaaat tcttaaatcc ttaaattcat cattttctaa taaaatagtc gtagttatac
    14341 ctccagaagt tttgattccc tctttttcaa agaactcctt tactttaaga attttctctt
    14401 tctctacatc cgaattccct cggtgagttt caagataaac ttttgatatc ttcaaatgct
    14461 ttttgaaaaa gttgaagcct tcttcgagct cgttgaaggt agcatccttc aaaaaaggtg
    14521 ccggacaata tagagccacc cggaaatttt tataatcctc catatcttct ccctctcttt
    14581 tttataacca atttttcgtc actttccctg aaaagaattt cagagtcaag catctcactc
    14641 cctgtattct cttcgtaaat ggatgacctc ccttctacaa aattttacat aagatctttc
    14701 atcacaaagc cttctgcttt tctattctat aaggatgtag aatttaatca gattttagca
    14761 taaaacagag cccgaaagaa acttcttgta attcctgaca tcattaccag acagtctttt
    14821 ccttgcctgt aatcctctcc aggctcatta tgagtcttta tatcccttcc gcggtaatac
    14881 tgtaattgct aatgtttagc gctaactttt aagttttgag gtacaatgaa gaaaatatct
    14941 aaaggggaga aagttatgaa agaggaaaat cttgcgggaa tgttagaaag tgtgcttgca
    15001 ggaagagtta aagaatataa gttagttgac cctaaaacag tggtagtggc tgaatgggtg
    15061 cgattcaaat gccagtttgg ttgtgatgga tacgcgcaga gttttacctg ccctccctat
    15121 tctccagagc cctcccgcac taaaagaatg cttgaagaat atgagttagc tgccatcctc
    15181 tggaaaccag acagttatga aaatctgcga aagattacta ctgatttaga gagaactctt
    15241 ttcctttccg gatactataa agccctggct attccctcag gaccatgtga gctgtgcaat
    15301 ccctgtcctt tggaatatcc ctgtcagcac cccgaatatg ctcgtccctc tatggaggcc
    15361 tgtggaattg atgtatatgc aactgtgagg caacatggtt ttccaattga ggtggtacgc
    15421 tcaagaaatt gccagcctaa ctactatgct ctcgttctaa tcgaatagag aagaaaaaac
    15481 aggaagaatg gctacgttat ttcactctga agcttcgttt cagttttcca cgaataagaa
    15541 aaaatggcta attgttctac tatttatcgg ggggatattt gtccgtttat ttttattggg
    15601 agttattcct ccgggattga atcaagatga ggcctccatt ggttatgatg cctgggctct
    15661 tctgcattat ggtatcgata gaaatgcttg ccacaacccc gttcatctta tttcctgggg
    15721 aagtggtcaa aatgcgcttt acgcttattt atccatgcct ttcattcttc tatttgactt
    15781 aaaccctttc tcggtaagaa ttttaaacgc cctgttaggg tgtttatctt cagttatctt
    15841 ttacctcctg gttaaaaaaa tctgggatga agatacaggg ataatagctc tattcctttt
    15901 ggttataagt ccctggcata taat
//
LOCUS       DAOSVE010000029        15745 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_2293_length_15745_cov_72.170236, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000029 DAOSVE010000000
VERSION     DAOSVE010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 15745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15745
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2293_length_15745_cov_72.170236"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(366..1538)
                     /gene="larC"
                     /locus_tag="PK016_03875"
     CDS             complement(366..1538)
                     /gene="larC"
                     /locus_tag="PK016_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HXL02243.1"
                     /translation="MGNFLYLDCFSGISGDMLLSALLDLGISASIFIPEIKKIPIPFE
                     IEIKKVQKHNISATQVKIKEEEHHHHRPARELIRIIQESPFPSSIKEKAQEIVGKIAE
                     VEARIHGTNPQEIHLHEVGGVDTLVDITGALIALHMLEIEEIRSSPIPTGHGWVETAH
                     GLLPIPAPATLELLRGIPIYSGEVEGELTTPTGAALISSLSKSFGSLPFCQIEKVGYG
                     AGERDYSYPNVLRAIVGKRETHLKEEKNLVIETNIDDMSPQIVEYLTEKLFQEGALDV
                     FVTPIFMKKQRPAFQLSVITSPFLKGKMEEVILKETTTLGVREYQVDKYFLPREEIEI
                     PTPWGKVRGKLSSFGEMIKITPEYEDCKKIAAQEKIPLLQVIQEAQKEGEKIAEGK"
     gene            complement(1538..2218)
                     /gene="larB"
                     /locus_tag="PK016_03880"
     CDS             complement(1538..2218)
                     /gene="larB"
                     /locus_tag="PK016_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HXL02244.1"
                     /translation="MRTYPFEDLGFAKIDYHRGIRKGFPEVIFCPGKTKEEILTIMQK
                     LANEYPNILATRANEEIYELVKSALPDARYHPQARAIFLEREPREKKGQIALVSAGTS
                     DLPVAEEAALTAEIAGSKIERIFDVGIAGLHRLLPYLPELWKANVIIAVAGMEGALPG
                     FLAGLVNRPIIAVPTSTGYGANLKGIAPLLTMLNSCSPGVAVVNIDNGFGAGFLAHMI
                     NQLAEGVR"
     gene            complement(2322..2876)
                     /locus_tag="PK016_03885"
     CDS             complement(2322..2876)
                     /locus_tag="PK016_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02245.1"
                     /translation="MVKIMFNRKKSFILILVVLELMVGSGGRAFSSVREAPVFTLYTL
                     KGEEVEFHPSNFTFLYFLSPDCEDCIYPLLALHQNLKENEKAKDITLLTICPDCSWRE
                     VKSLKETLPSEIEIYFGNYLKALWGVWELPSLFLVNSQNKVVEKWEGNLDTEKILARL
                     PQPDNNKKSEKKKATSSCPQELCY"
     gene            complement(2896..4089)
                     /locus_tag="PK016_03890"
     CDS             complement(2896..4089)
                     /locus_tag="PK016_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02246.1"
                     /translation="MKRIEIITIIIIMVLFISVSEGKERALFLDSTSSQVFAPLHLFL
                     ERGERWEWVEAPPGLQEAENLDFSELQKKGDLLFRGYEKDDLLFFEVLDLKTSHKILA
                     LVRKKEDQDYMLKELKEKLAREFAFHREIKIIFVREEYGENSIVFSDIEGRKQISVYT
                     TEEGYIEKPLLSPDARYLIFVLDKGWDKGIYRLDLENGKVEALSEAGFKDYSPALSSR
                     GNKIVFVSERKNKQSIFQMNGDGSSQKLLIDKDNPLQWPAFSPDDRRLAYCELIDGKW
                     TLKVYDLLAGEEKIFDFPLNVKQPVFTSEGKRILFVGEEEGNSDIYLLDLVNQNLTRL
                     TYDNSPKENPFPSPDNSWVAFSSQKEGNNWDIFLLDMAKGTVKRLTSSWSREADPSFS
                     PLPIY"
     gene            4154..5077
                     /locus_tag="PK016_03895"
     CDS             4154..5077
                     /locus_tag="PK016_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HXL02247.1"
                     /translation="MANFSIITISREVESYGEEIAEKLSQRLKVELVDKERFVSFLKE
                     ISPQFPAEPPQDEKERDQWIIKVRSFLHQKASQRPFILVGRGGQKILENCSFAFHVLI
                     VASQVTRLHRMMEKYQQDDVTASRILTEQDQQRERFLRQTFGIDWREPLHYHLVVNTD
                     FYGVNEAADLILEAQDKVATPPQPRKVEQQPLFPREDLGQKTSFSHPSEKEFARMLDF
                     YGVRWLYEPRTFPLEWDEEGNLAEAFAPDFYLPDFDLFIELTTQRQKLTRKKNRKVRR
                     LKELYPQVKIRVVYARDYEYILNHLKGENNE"
     gene            5070..5642
                     /gene="hpt"
                     /locus_tag="PK016_03900"
     CDS             5070..5642
                     /gene="hpt"
                     /locus_tag="PK016_03900"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HXL02248.1"
                     /translation="MSERRLGSLLFGREEIEERIEEVAKDISLDYESRRPVLAVILKG
                     AVYFAVDLTRNLEISFDLDFLALSGFKPGLLRVEIEKDLDIDIKEREVLIIEDIVDTG
                     LTLNFLIRELEKRFPRSIRICTFLDCPARRIADIPIHYRCFEIPDVYVVGYGLDFQGN
                     FRNLVEVYTLYDQSGRSTQILKQMGKGGKK"
     gene            5639..6691
                     /gene="hisC"
                     /locus_tag="PK016_03905"
     CDS             5639..6691
                     /gene="hisC"
                     /locus_tag="PK016_03905"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HXL02249.1"
                     /translation="MSYFREVINFMEEYIPGEQPEEEGYIKLNTNENPYPPSPLVEEK
                     IKEALSEGRISLYPPPLGGKLREKAQEVYGLPRDWIAVGNGSDELLNLLFRLFLGKGD
                     RVAYPFPTYTLYRTLAFLQEAEREEIPFPPDFSLPDSFLGCSAKLKVVSNPNSPTGTF
                     LSLSQIEKLLSLSSCPVIVDEAYVDFAPYSVFSLLQDFPNLIIVRTLSKSFSLAGLRV
                     GLLFASPDIVRGILKIKDSYNVGILPQVGGAAALEDIEYMKKNVEKIKSTREWFSEEM
                     RQLGFLVYPSQANFIMVKKERRDLKFLYEELKRRKILIRYFPEWPDSLRITIGKEKEM
                     KILLAAIQEIIEGVNV"
     gene            6684..6968
                     /locus_tag="PK016_03910"
     CDS             6684..6968
                     /locus_tag="PK016_03910"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HXL02250.1"
                     /translation="MFKLFIWDLDNTLIASSSLLWGAFSWVAEEYGNKKMTPQEIVSL
                     YGPPEDVVIEKIVGKEKKEERRRKRLFRNSIVSIRKSMMSWLKFSLSCWK"
     gene            6914..7345
                     /locus_tag="PK016_03915"
                     /pseudo
     CDS             6914..7345
                     /locus_tag="PK016_03915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
     gene            7358..9406
                     /locus_tag="PK016_03920"
     CDS             7358..9406
                     /locus_tag="PK016_03920"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HXL02251.1"
                     /translation="MSWEKVRKRNGELEDFSPTKITRAIYKALSACGRGSEEEAENLT
                     QKVLKILPERVKSSIPEVEEIQDAVEETLMREGFPEVARAYILYREKRRELREAKQKL
                     FGVKDDLKLSLNAVQVLQERYLLRDEEGRIIETPREMMRRVAKAIAQVDARYGEDVEK
                     TCSEFYEMLTSLYFLPNSPTLMNAGTPLGQLSACFVLPVEDSIESIFDALREMALIHK
                     SGGGTGFTFTHLRPRGDRVSTTGGRASGPVSFMRIFDTATEVVKQGGKRRGANMGILR
                     FNHPDVREFIHAKEKANFLENFNLSVAITDEEVEKVQRGEELELRNPKDEKVWEKINA
                     RELFKMLAESAWRSGEPGLIFLDEINRHNPTPSQGRIEATNPCGEVPLLPYESCNLGS
                     INLARMVEEGKIDYERIKETVKRAVHFLDNVIDANLYPLPQVQKMVEGNRKIGLGVMG
                     FADMLVKIGVSYYSEEALKIAEEVMNFISFQARFASQELAEKRGTFPNYPQSIWADKD
                     LSLRNATLTSIAPTGSISLIASVSSGIEPFFALAFRRYVLDNTELLEINPLLEEKLKS
                     YQIPEETWKIIWEKGNLEGIDVPEELHRLFITALEIPPAFHIRMQATFQKYVDNAVSK
                     TINLPASATPQDVEEAYLLAQELKCKGITVYRYGSRPQQVLYLGVEEKSCETCWRRIF
                     "
     gene            complement(9312..10547)
                     /locus_tag="PK016_03925"
     CDS             complement(9312..10547)
                     /locus_tag="PK016_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02252.1"
                     /translation="MAKILLISNGYGENSFSSLLLESLIQEIEKEKLPLKVETVSLVG
                     EGKIFDPWLSHPQVSILHSSPPLPYGGVYLGGIRERTTHFIKDFQIQRKNLRAIKKKL
                     APLQDEIDWVIGVGDFLPVLISKLFLKKKVFLVACYHSHLLKNSKKPYEKIGRYTAYL
                     FRHYTFRVYTRDYPTAKWFKSLGINAHYLGFLGPSPSILVPQRREIILLTGSREDWKE
                     NLQFLAKTLQYISPSLLQSFSIHWVFPPERKEEEIEREMQKVKGSFTFSRGDYLEHLS
                     QGALVVGFAGTAMEQAAFLGIPSLQPYREKAIQSNPYFINKRQKLLLGDALITGADTP
                     QETAKILEEALVNLSFHQKRAQEFSEKTWGRKREGAKNIAQDLAKEFKKSFSNKFHKT
                     FPPPPDTVPVEVDYRSDRR"
     gene            11336..12406
                     /gene="thrC"
                     /locus_tag="PK016_03930"
     CDS             11336..12406
                     /gene="thrC"
                     /locus_tag="PK016_03930"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HXL02253.1"
                     /translation="MRWPGVLEYFAEYLPVTSHTPLITLNEGNTPLVESYHLVKKLGV
                     GKLYFKFEGMNPTGSFKDRGMVVAVAKAKEEGRSVVMCASTGNTSASAAAYAQRAGLS
                     CIVLIPQGKIALGKLSQALMHGARVLAVRGNFDDALSMVRYITSHYPVALVNSINPYR
                     IEGQKTAAFEVVEWLGESPAYLALPVGNAGNITAYWKGFKELKERGKINSLPRMLGFQ
                     AEGAAPLVSGKPVPEPQTVATAIRIGNPASWKGAIAARDESGGVIEAVSDEAILQAQR
                     MLGEEEGLFVEPASAASLAGVLKKSREGFFTGRETVVCVLTGHGLKDPDIAIKQGEGR
                     IEEVGASKEEVVEILGLEGQDV"
     gene            12399..13298
                     /gene="thrB"
                     /locus_tag="PK016_03935"
     CDS             12399..13298
                     /gene="thrB"
                     /locus_tag="PK016_03935"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HXL02254.1"
                     /translation="MFEVQVPATSANLACGFDTLGLALNLFYRVEVSSADEGFHLYHE
                     GEGKGIIKEEDNLFFQAVKKTWEKVGFSGLGMRVRAFNEIPISRGLGSSAACIVAGVT
                     SANELMGRVLSLSQMIELASQIEGHPDNVLPAFLGGFTIAFSKEGKIFYHQLAFLPEV
                     EIVVCVPDYTLSTEEMRKILPPSYPRDKVVFNLSSLAYLLGSIFTEDKEGFLLSLEDE
                     IHQPYRGAKIKGFCEVKEYIQKRNLGNVAISGSGPTLILFLSRPLQDKEEKDVEGIFQ
                     SQGVNINIKKVEWVKEGVKVRKL"
     gene            13295..14527
                     /locus_tag="PK016_03940"
     CDS             13295..14527
                     /locus_tag="PK016_03940"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HXL02255.1"
                     /translation="MSLIVQKYGGSSLADLDKIRLVASRVKKYGNEGHDLVVVVSAMG
                     KTTDQLISLAKSLHPSPPERELDMLLSTGEQVSIALLAIALSSLGVKSVSLTGGQAGI
                     ITTDTYTKAKIFHIDTRRIKELLLNHQVVIVAGFQGRTSQGDITTLGRGGSDITACAL
                     AAALNADFCEIYTDVPGVFTADPHLVPQAQKIARLSYEEMSEMATLGAKVLQPRAVDF
                     SQAYRVRVHVRSTFSEEEGTWVEELNNMEEVRVKAITHSGDMLKIVLLGVPNQPGVAA
                     QIFKTLSDFSVFIDMIIQSEGREDYKDLAFVVSREDEDKVKKAISSLEKELPFLGMKF
                     DETMSRVSLVGAGIASNPNIAYRMFNALAQAGINIDMISTSNSRISCLIPRDLMGEAV
                     KALHEEFIIQEVKLNEGI"
     gene            14514..15530
                     /locus_tag="PK016_03945"
     CDS             14514..15530
                     /locus_tag="PK016_03945"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HXL02256.1"
                     /translation="MKEFRVAVVGVTGAVGQEMLKVLEERKFPVSEIRPLASSRSQGK
                     KVNFRGREWTVEVLDQNSFRGVDIALFSAGASISREFAPLAAQSGCVVIDNSSAFRQD
                     PDVPLIVPEINPHRIKDYRQRNIIANPNCTTIISLMPLKPLHDYGKIKRVIAASYQAT
                     SGAGAKAMAELEEQTKAYISQEEIVPHVFPQQIAFNVIPHIDVFLDNYYTKEEMKMVW
                     ESRKILEEENLLISATCVRVPVLRAHAVAINIETEQKITRDKAIELLSSFPGVKVVDD
                     PEHLLYPTPLMAAGKDECLVGRIREDISSPLGLNLWVVGDQLRKGAALNAVQIAELLA
                     PEIS"
     gene            15579..15662
                     /locus_tag="PK016_03950"
     tRNA            15579..15662
                     /locus_tag="PK016_03950"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15612..15614,aa:Leu,seq:tag)
BASE COUNT         4591 a         2920 c         3504 g         4730 t
ORIGIN      
        1 ccagtagttt ttgtttattt gctttttaag tctttgttct tttcttttgc tttttggggg
       61 attataaagg ggcacagccc ctttatagtt cggggttata agggggcagc gcttcactga
      121 ggaaggtttt ttcttcctca gtagtttttg agtctttact tttatatttt tcaagtttta
      181 atcttttttg ggggttatga ggggggtctt ttcccccctc atagtcttta taatggattt
      241 tgcggttaac tataacccca ctattagact tctctggttg agtttcagag ttagctctta
      301 tagtatgcat tttttgatgg ctcttacctt taaaattctt tccgtgggga agtggcaagt
      361 taagactatt tcccttcggc gattttttct ccctcttttt gggcttcttg tatcacctgg
      421 agaaggggta ttttctcttg agcagctatt tttttgcagt cttcgtattc tggggtaatt
      481 ttaatcattt ctccaaaaga agataattta cctcttactt taccccaggg ggtgggtatt
      541 tctatctcct ctcgaggaag aaagtattta tctacttgat attctctcac tcccagggtg
      601 gtagtttctt ttaaaattac ttcttccatt ttacctttta agaaaggaga tgtaattacc
      661 gagagctgaa aagctggcct ttgctttttc ataaaaatgg gagtaacgaa aacatccaga
      721 gctccttcct ggaaaagctt ttcagtgagg tattctacaa tctgcggtga catatcatcg
      781 atattggttt ctattaccag gtttttttct tctttaagat gagtctctct tttccccact
      841 atagctcgca agacattagg ataagaataa tctctttctc ctgctccata tcccactttt
      901 tctatctggc agaaaggtaa gctgccaaag gatttgctca aagaagatat aagggctgcc
      961 ccagtagggg tagtcagctc tccttctact tctccagaat aaataggtat acctcgcaaa
     1021 agctctaagg tagcaggagc ggggataggt agtaagccat gagcagtttc tacccaacca
     1081 tggcctgtgg ggataggaga agaacgaatc tcttctattt ctaacatatg cagagctatt
     1141 aacgctccgg ttatatctac taaagtatcc actcctccta cttcatgaag gtgaatttcc
     1201 tgggggttag ttccatggat tctggcttct acttcggcaa ttttccccac gatttcttga
     1261 gccttttctt ttatggagga gggaaaaggg ctttcctgaa tgatacgaat taattccctc
     1321 gctgggcgat gatggtgatg ctcttcttct tttattttaa cctgagtagc agagatattg
     1381 tgtttctgga cctttttgat ctctatctca aaagggatgg gtatcttttt aatttcagga
     1441 ataaaaatgg aagcagaaat tcccagatct aagagagcac tcaaaagcat atctccgctt
     1501 attcctgaaa aacaatccag ataaaggaaa ttacccatta tctcacccct tcagctaact
     1561 gatttatcat atgggctaaa aatccagctc caaacccatt gtctatattt accaccgcca
     1621 ccccgggaga acagctatta agcatagtaa ggaggggagc aatccctttc aagttggctc
     1681 catatccagt actggtagga acagcaataa taggtctatt tactaaccca gctagaaacc
     1741 cgggtagagc tccttccatc cctgctacgg cgatgatgac attggctttc cagagctcag
     1801 gaagataagg taacaggcga tgcagaccag cgatacccac atcgaagatt ctttctattt
     1861 tacttccggc aatttcagca gtgagtgctg cttcttctgc tactggtaag tcagaagttc
     1921 cagcactcac cagagcaatt tgccccttct tttctcgagg ttcccgctct aaaaatatgg
     1981 ctcgggcctg agggtggtag cgagcatcgg gtagagctga tttaaccagc tcataaattt
     2041 cttcattagc ccgggtggct aaaatattgg gatattcatt agctaacttt tgcattatag
     2101 ttaatatttc ttctttagtt tttcccggac agaaaatcac ttccgggaat cctttgcgaa
     2161 tacctcgatg gtaatcaatt ttggcaaacc ctaagtcttc aaaagggtaa gttctcaatt
     2221 tttgtttggc ttcctcagga gttatttttc tctcccagag atcttttaat atttcttccc
     2281 acttttctgg aatggtattt tccccctttt ctttttatga tttagtaaca taattcttga
     2341 ggacaagaag aagtagcttt tttcttctct gattttttat tattatcggg ttggggcagg
     2401 cgagccaaaa tcttttccgt atcgaggttc ccttcccatt tttctactac tttgttttgg
     2461 gagttgacca gaaacagtga aggtagttcc cagacccccc acagagcttt taaataatta
     2521 ccaaaataaa tctctatttc agagggtaac gtctccttta aactcttaac ctctcgccag
     2581 ctacaatcag ggcatatggt taaaagagtt atgtctttag ccttttcgtt ttcttttaaa
     2641 ttttggtgga gggcaagaag aggataaata caatcctcac aatcaggaga caaaaaatac
     2701 agaaaagtga agttggaagg atggaattcc acttcttcgc cctttaaagt ataaagagta
     2761 aaaacaggag cttctcttac agaagagaaa gccctgcctc ctgaacccac cattaactcc
     2821 agaactacta agattaaaat gaaactcttt ttccggttga acataatttt taccattata
     2881 atggtaatct gttctttagt aaatcggtaa aggagagaag ctgggatcag cttctcgact
     2941 ccaggaggaa gtaagacgtt ttaccgtccc ctttgccatg tctaataaaa atatgtccca
     3001 gttgttaccc tccttctgag aggaaaaagc tacccaacta ttgtcaggag agggaaaagg
     3061 gttttctttg ggggaattat cataggtgag ccgggttaaa ttctggttta ccaggtccaa
     3121 taaatatatg tctgaattac cctcttcttc tcctacgaaa aggattctct taccttctga
     3181 agtaaaaaca ggttgtttta cattcagggg aaagtcaaaa attttttcct ccccggctaa
     3241 aaggtcatac acttttaatg tccattttcc atcaattagc tcacaataag ccaggcgtcg
     3301 gtcatcggga gaaaaagctg gccactgaag aggattatct ttatctatca gtagtttttg
     3361 ggaagagccg tctccattca tctggaaaat actctgttta ttctttcttt cagaaacgaa
     3421 aactatttta ttgcctcgag aagaaagggc tggagagtaa tccttaaaac cagcttcgct
     3481 tagtgcttct acttttccat tttctaagtc gagccgataa atgcctttgt cccagccttt
     3541 atctaaaacg aatatcaaat agcgagcatc gggagataag agaggttttt ctatataacc
     3601 ttcttcggtg gtataaacgg aaatttgttt tcttccttct atatcggaga aaacgataga
     3661 gttttctccg tattcttctc tgacaaagat gattttaatt tcccgatgaa aggcaaattc
     3721 ccgagctagc ttctctttta gctcttttaa catataatcc tgatcttctt tctttctcac
     3781 caaagccaga atcttgtggg aggtttttaa atccaggacc tcaaaaaata gtaaatcatc
     3841 tttttcataa ccccggaata gcaggtctcc tttcttttga agctcagaga aatcgagatt
     3901 ttctgcttcc tgtaagccag gaggtgcttc tacccactcc catctttcac ctctttctaa
     3961 aaagagatgt aggggtgcaa agacttgaga agaagtagaa tcgaggaata atgctctctc
     4021 ttttccttct gatactgaaa taaataagac cattattata attattgtta ttatttctat
     4081 ccttttcact tcatcgcccc cttttttata aatcttttat ttgatagtat aatataaaac
     4141 tggtgaaaag aaagtggcta atttttctat tattactatt tctcgagaag tagaaagcta
     4201 tggggaagag attgccgaga aactttctca gagattgaaa gtggagctgg tggataaaga
     4261 aagattcgtt tcttttctaa aagaaatttc tcctcaattt cctgctgagc ctcctcaaga
     4321 cgagaaagaa cgggaccaat ggattataaa agtacgctct tttctccatc aaaaggcttc
     4381 tcagagaccc tttattttag tggggagagg agggcagaaa attttagaaa attgttcctt
     4441 tgcctttcat gttttaatag tggcttcaca agttactcgt ctacaccgga tgatggagaa
     4501 atatcagcag gacgatgtga ctgcaagccg gattcttact gaacaagacc agcaaagaga
     4561 aagattttta aggcaaactt ttggaataga ctggagagaa cctcttcact accatctggt
     4621 agtcaatact gacttctatg gtgtaaacga agctgccgat ttgattttag aagcccagga
     4681 taaagtagca actcctcctc aacctagaaa agtggagcaa caacctcttt tcccccggga
     4741 ggatttggga cagaaaacgt ctttttccca tccttcagaa aaggaatttg cccgaatgtt
     4801 agacttttat ggtgttcgct ggctttatga gccacgtacc tttcccttag agtgggatga
     4861 agaaggcaac cttgctgaag cttttgctcc tgacttttac cttcctgact ttgacctttt
     4921 tattgaatta actactcaga gacagaagct aactcgaaag aaaaatcgaa aagtgagaag
     4981 gttaaaagaa ctttatcctc aggtaaaaat ccgggtggtc tatgctcgag attatgagta
     5041 tattttaaat catttaaagg gagaaaataa tgagtgaaag acgcctagga agcttgctat
     5101 ttggaaggga ggaaattgaa gaaagaattg aggaggtggc aaaagatatc tccctggatt
     5161 atgaaagcag aagaccagtg ctcgcagtga ttttgaaagg agcagtttat tttgccgtag
     5221 atttaactcg aaatttagaa atatcttttg accttgattt tctggctctt tcagggttta
     5281 aacctggctt attacgagtg gagatagaaa aagacctgga tatagatata aaagaaagag
     5341 aagtattgat aatagaagat attgtagata ctggtttaac tctcaatttt ttaattcgag
     5401 aattagaaaa gaggttcccc cggagtataa gaatttgcac ttttctggat tgcccggcac
     5461 gaaggattgc tgatattcct atacattatc gttgttttga gatacctgat gtctatgtgg
     5521 tgggttatgg tttagacttc cagggtaact ttcgtaattt agtcgaggtt tatactttat
     5581 atgaccaaag tggaagaagt acccagattt tgaagcagat gggaaaggga gggaaaaagt
     5641 gagctatttt cgagaggtaa tcaattttat ggaagaatat attcccggtg aacagccaga
     5701 agaagaagga tacataaaac ttaataccaa tgaaaacccc tatcctcctt ctcctctggt
     5761 agaggaaaag attaaagagg ctctgagcga aggtagaatt tctctttacc ctcctcctct
     5821 gggagggaaa ttgagggaaa aagctcagga agtttatgga cttccccggg attggatagc
     5881 ggtggggaat ggttcggatg aacttttaaa tctacttttt cgcctttttt taggtaaagg
     5941 agaccgggtg gcttatcctt tccctactta tactctttat cggacgttag cttttcttca
     6001 ggaagcagag agagaagaaa ttcccttccc tccggatttt tctcttcctg atagtttctt
     6061 aggttgttca gccaagttaa aagtagtctc taaccctaat tcacccactg gtaccttcct
     6121 ttctctatct cagatagaaa aattactttc tctttcttcc tgtcccgtaa tagtagacga
     6181 ggcttatgtt gattttgctc cttatagtgt tttttctctt cttcaggatt ttcctaatct
     6241 tataatagta agaactcttt ccaagtcttt ttccttggct gggcttcgag tggggcttct
     6301 ttttgcttct cccgatatag taaggggaat tttaaaaatt aaagattctt acaatgtagg
     6361 aatcttacct caagtgggag gggcggcagc tttagaagat atagaatata tgaaaaaaaa
     6421 tgtagaaaaa attaaatcca cccgggaatg gttttccgaa gagatgaggc aactgggctt
     6481 tttagtttac ccttcccagg ctaattttat tatggtaaag aaggagagaa gagatttaaa
     6541 gttcctttat gaagagctaa aaaggagaaa aatactcatt cgctattttc ctgagtggcc
     6601 ggactctttg cgtattacca taggaaaaga gaaagaaatg aaaatcctcc ttgctgctat
     6661 acaggaaata atagaagggg taaatgttta aactttttat ctgggattta gataatactt
     6721 tgatagccag ttcttctctt ctgtggggag ctttttcctg ggtagcagag gaatatggaa
     6781 ataagaaaat gactcctcag gaaatagtta gtctgtatgg tccacctgaa gatgtggtta
     6841 tagaaaaaat agtaggaaaa gagaagaagg aagagagaag aaggaagagg ctcttcagga
     6901 attctatcgt ttctatcagg aaaagcatga tgagctggtt aaagttttcc ctctcttgct
     6961 ggaaataatt aactatcttc gagaacgtgg ggtaaaacaa gctcttttta cctccaaggg
     7021 aagaaaaagt gctgaaataa cgttgcaaaa gctgaatata aaggattttt ttgaccttgt
     7081 attatgtgga gacgaagtgc ttcatcccaa accttatccc gatggggtga taaaaatttt
     7141 aaaaactcta ctggttgagc cagaggaaac tctatatctg ggagattctc ctctggatgc
     7201 agaagccgcg tacaaagcag gagtgcattt tgctcttgct ctctgggact ttcttcctcg
     7261 agaaaataaa agggaagaag aggcagagtg ggtgttccat tctcccgaag agctctggga
     7321 atgggtgcag aaaatttatg attgaggagg gataattttg tcctgggaaa aagtgagaaa
     7381 aagaaatggg gaattagaag atttttctcc gacaaaaatt actcgagcta tttacaaagc
     7441 tctctctgct tgtggaaggg gaagcgagga agaagcagaa aatcttactc agaaagtatt
     7501 gaagattcta ccggaacggg tgaaatcatc tatacccgag gtagaagaaa tccaggatgc
     7561 ggtggaagag acgctgatga gagaaggatt tccagaagta gcccgagctt atattctcta
     7621 tcgagaaaaa agaagagagt tgcgagaagc gaaacaaaaa ctttttggag tgaaagatga
     7681 ccttaagctt tctctcaatg ctgtgcaagt gctacaggag cgctatcttt tgagagatga
     7741 agaaggaagg ataatagaga ctccccgaga aatgatgagg cgggtggcaa aagctatagc
     7801 tcaagtagat gctcgttatg gagaggatgt agagaaaact tgctctgaat tttacgaaat
     7861 gctgacttct ctttactttt tgcccaattc tcctactttg atgaatgccg gtactcctct
     7921 cgggcaactt tcggcttgtt ttgttttgcc agtagaggat tccatcgaat ctatatttga
     7981 tgccttgcga gaaatggctc ttatccataa atccggggga ggaacaggtt ttacttttac
     8041 tcatcttcgt cctcgaggag acagagtttc tacaaccggt ggtagagctt caggaccggt
     8101 ttcttttatg agaatttttg ataccgctac tgaagtagta aaacagggcg ggaaacgccg
     8161 gggagccaac atgggtattt tgcgcttcaa ccatcctgat gttcgagaat ttatccatgc
     8221 taaagagaag gctaattttt tagaaaattt taatctttct gtagctataa ctgatgaaga
     8281 ggtagagaaa gtacagaggg gagaggagct ggaactccgt aaccccaaag atgaaaaagt
     8341 gtgggagaaa ataaacgccc gagaactttt taaaatgtta gccgaaagtg cttggagaag
     8401 tggagaaccg ggtcttatat tcttagatga aataaaccgt cataatccca ctccctctca
     8461 aggtagaatt gaagctacta acccctgtgg agaagttcct cttttacctt atgagtcctg
     8521 taatttaggc tccattaatt tagctcgtat ggtggaggag ggaaaaatcg attatgaaag
     8581 gataaaagaa acggtaaaaa gagcagtgca ttttttagat aacgttattg acgctaatct
     8641 ttatcctctt cctcaagttc aaaaaatggt agagggaaac cgaaaaatag gtctgggagt
     8701 gatggggttt gccgatatgt tggttaaaat cggagtttct tattatagcg aagaagcctt
     8761 gaagattgct gaagaagtga tgaattttat ttcttttcaa gctcgatttg cctctcagga
     8821 actagctgag aaacggggaa ctttccccaa ttatcctcaa agtatctggg cagataaaga
     8881 tttatccctt cgcaatgcca ctctcacctc tattgctcct acggggagta taagtttaat
     8941 tgcttcggta tccagtggga tagagccttt ttttgctttg gcctttcgtc gctatgttct
     9001 agataatacc gagcttttgg aaataaatcc tctgttagaa gaaaagttaa aaagctatca
     9061 gattccggag gaaacctgga aaataatttg ggaaaaggga aacttagaag gaattgacgt
     9121 tccagaagaa ctgcatcgtc tctttatcac tgccctagaa attcctcctg cttttcacat
     9181 tcggatgcag gctactttcc agaaatatgt agataatgct gtttctaaaa ccattaacct
     9241 cccggcaagt gctacccctc aagatgtgga ggaggcatat cttttggctc aagagctaaa
     9301 atgtaaagga attaccgtct atcgctacgg tagtcgacct caacaggtac tgtatctggg
     9361 ggtggaggaa aagtcttgtg aaacttgttg gagaaggatt ttttaaattc tttcgctaaa
     9421 tcctgggcga tattttttgc cccttctctt tttcttcccc aggttttttc gctaaactct
     9481 tgagctcttt tctggtggaa ggagagatta acgagggctt cttctaagat tttagcagtt
     9541 tcttgaggag tatctgctcc ggtaatgaga gcatcaccga ggaggagttt ctggcgtttg
     9601 tttataaagt agggattact ttgaatagct ttctctcgat agggttgtaa ggagggaata
     9661 cctaaaaaag cagcttgttc catggctgtg cccgcaaacc ccacaactaa tgctccttgg
     9721 gagagatgtt ctaaataatc tcctcggcta aaagtaaagc ttccctttac tttctgcatc
     9781 tctctttcta tttcttcttc ttttctctcg ggcgggaaaa cccagtggat agaaaaagat
     9841 tgcagaaggg aaggagaaat atattggaga gttttagcta aaaactggag attttctttc
     9901 caatcttctc gactcccggt aagcaaaatt atttctctcc tttggggaac caatatagaa
     9961 ggtgagggac ctaaaaatcc tagatagtgc gcattgatgc ctaaactctt aaaccactta
    10021 gctgtaggat aatctcgagt atagactcgg aaagtgtagt ggcggaaaag ataagcagta
    10081 tatcttccta ttttctcata aggtttttta ctatttttta gaagatgact atgatagcaa
    10141 gctacgagaa aaactttctt ttttaaaaat aacttactga taagaacagg taaaaaatcg
    10201 cccactccaa ttacccaatc gatttcatct tgcaaaggag caagcttttt cttaatcgct
    10261 cttaaatttt tcctctggat ctggaagtct ttaataaaat gggtggttcg ttctcttatc
    10321 cctcctagat atacaccccc ataaggtaga ggaggagaag aatgcaatat cgatacttga
    10381 ggatgagaaa gccagggatc gaaaatcttt ccctctccca ccaaagacac agtttctact
    10441 tttagaggaa gcttctcttt ctctatctcc tgaattaagc tttccaataa gagactggag
    10501 aaagagtttt ctccgtatcc attgctaatt aaaagtattt tagccatcac ctcaatttta
    10561 aacattagtg cttaaaatag caagacaatc tacgacagac gataaggggg caagccccct
    10621 tataaaccct cgaaaaacaa aacataaaga taaactatga aaaagatcaa aaatagcccg
    10681 gagaaaaaac ctctgtgtgt cacccctgga tggttctacc atcacttatc tatcattcct
    10741 gaatgcttct atcaggaatc cgaactatag ggggaaaccg atccccctat tacccccttg
    10801 gaaaaacaaa aaacacaaag aataaagact actgaggaag ggaaaatatc ttcctcagtg
    10861 gggtgccccc tttataatcc cccaaaaaag gaaaaaataa agaataaaaa attaaaagat
    10921 taaaggcaaa aaaactgagg gagaaaggct tccctcagtg gattcccgat tactgatttt
    10981 gggaatgacg ggaggaggga ggccacccct gaatgattcc aacaggggcc tgcagctttt
    11041 aaaaacatct ggatctcaat aaaagatttc aggtgtgatg gaacaatgct ataaggttaa
    11101 aagatccttc tttttccttt cccttccggg gagagagata gaatgaggat gttttcctaa
    11161 aatattctct ctttttatcc ccggaaagat gaaaagagag aaatcgtctt tatcaagact
    11221 tgtagctaac cgtaaatctc cttttagttt ttaaatttag tattgaaaac tggtgattca
    11281 gaagaatagt tgtggtatgc taatgaaaat ttgttttagt gaaggaggtt taactttgcg
    11341 ctggcctgga gtattagagt attttgctga gtatcttcct gtgacttctc acactccttt
    11401 aattacttta aatgagggaa acactcctct ggtcgagtct tatcatttgg taaagaaatt
    11461 aggagtagga aagctttatt ttaaatttga agggatgaac cctactggct catttaaaga
    11521 ccgggggatg gtggtggcag tggccaaagc caaagaagag ggtagaagtg tggtgatgtg
    11581 tgcttctact ggcaatactt ctgcttcagc tgcagcttat gcgcagcgag ctggcctttc
    11641 ctgtatcgtt ctcattcctc aggggaaaat tgctctgggt aaactttctc aggctcttat
    11701 gcatggagca cgagttctcg cggtgcgggg aaactttgac gatgctctat ctatggtaag
    11761 gtatataact tctcactatc cggtggctct ggttaactcc attaatcctt atcgtattga
    11821 aggacaaaaa actgcagctt ttgaagtggt agagtggctg ggggaaagtc ctgcctatct
    11881 tgcgctcccg gtgggtaatg cgggaaatat cactgcttat tggaaagggt ttaaagagct
    11941 aaaagagaga ggaaagatta actctctccc tcgaatgtta ggttttcaag ctgaaggagc
    12001 agctcctttg gtttctggaa aacccgttcc tgagcctcaa actgtagcta ctgctatccg
    12061 gattggcaat ccggccagct ggaagggagc tattgcggct cgggatgagt cagggggtgt
    12121 gattgaagca gtgagtgatg aagcaatttt gcaggcgcaa aggatgctgg gagaagaaga
    12181 ggggctattt gtagagccag cttctgcagc gtctttggct ggagtgctga aaaaatcccg
    12241 ggaaggattt tttaccggta gagaaaccgt agtttgtgtt ttaaccggac acgggcttaa
    12301 agaccccgat attgccataa agcaaggtga gggaagaatt gaagaagtag gagcatctaa
    12361 agaagaggta gtagaaattt tggggctgga ggggcaagat gtttgaagta caggttccag
    12421 ctacctcagc gaatttagct tgcgggtttg acactttagg cttggctctt aatctctttt
    12481 accgggtaga ggtaagtagc gcagatgagg gtttccactt gtatcacgaa ggagagggaa
    12541 agggaataat taaagaagaa gataacctct tctttcaggc agtgaagaaa acctgggaaa
    12601 aagtgggttt ctccgggctg gggatgagag taagagcttt taatgagata cctatttccc
    12661 ggggtttagg aagtagtgcg gcatgtattg tagctggagt tacctctgcc aatgagctaa
    12721 tgggtagagt tttgagttta tctcagatga tagagttagc ttcgcagata gaagggcatc
    12781 cggataatgt tttacccgcc ttcttaggag ggtttaccat agctttttct aaagagggaa
    12841 agatttttta tcatcagctt gcttttttac cggaagtgga aatagtagtt tgtgttcctg
    12901 actacactct ctctactgag gaaatgagaa aaattcttcc cccttcttat cctcgggata
    12961 aagtagtttt taatttatcg tctctggctt atcttttggg aagtattttt actgaagata
    13021 aagaaggatt ccttttgtct ttagaagacg agattcatca accctatcgg ggagctaaaa
    13081 ttaaagggtt ttgcgaggtt aaagagtata tccaaaagag aaatttgggg aatgtagcca
    13141 ttagtggttc agggcctact ttaatccttt ttttgtcccg gcccttacaa gataaagagg
    13201 aaaaagatgt ggaagggatt tttcaatctc agggagtaaa tataaacata aaaaaagtag
    13261 aatgggtgaa agaaggagta aaggtgagga agctatgagc ctcatagtgc aaaaatacgg
    13321 cggctcttct ttagctgact tggataaaat cagattagta gcttcccggg taaaaaagta
    13381 tgggaatgaa gggcacgatt tggtagtagt agtttcagct atgggaaaaa ctactgatca
    13441 gctcatttcc ctggctaaga gtttgcatcc ttctcctcca gaaagagagc tggatatgtt
    13501 gctttctacc ggggaacaag tttccatagc tctcttagct attgctcttt cttctttagg
    13561 agtgaaatct gtgtccttaa ccggggggca agcaggaatt attaccactg acacttatac
    13621 taaagctaaa attttccaca tagatacccg gagaataaag gagctccttc tcaatcacca
    13681 ggtggtgata gtggctggct ttcaggggag gacttctcaa ggagatatta ctactttagg
    13741 aagaggagga agtgatataa ctgcttgcgc tttggcagca gctctgaatg ctgatttctg
    13801 cgagatttat accgatgttc cgggggtatt cactgccgat ccccatcttg ttcctcaggc
    13861 ccaaaaaatt gcccgtctct cttatgaaga aatgtcagag atggctacct taggagctaa
    13921 ggttttgcaa ccccgggcag tagatttttc tcaagcctat agagtgagag tacatgtacg
    13981 ttctactttt tcagaggagg agggaacctg ggtggaagag ttgaataata tggaagaggt
    14041 aagggtaaag gccatcactc acagtgggga tatgctaaaa atagtattat tgggagtccc
    14101 taaccaaccg ggagtggcgg ctcagatttt taagacttta tctgattttt cagtttttat
    14161 cgatatgatt attcagagcg agggaagaga ggactacaaa gacttagctt ttgtggtttc
    14221 tcgagaagat gaagacaaag ttaaaaaagc cattagtagc ttagaaaagg aattgccttt
    14281 tctagggatg aagtttgatg aaacgatgag ccgggtgtct ttagtaggag caggaatagc
    14341 cagcaaccct aatattgctt atcggatgtt taacgctctg gctcaggcag ggataaacat
    14401 agatatgatt agcacttcta attctcggat ttcttgtctc ataccccggg atttgatggg
    14461 agaagcggta aaagctttgc acgaagagtt tattattcag gaggtgaaac tcaatgaagg
    14521 aatttagagt ggcagtagta ggagtaactg gggcagtagg gcaggagatg ctcaaagttt
    14581 tagaggagag gaagtttccc gtttcagaga ttcggccttt agcttcttct cgttctcaag
    14641 ggaaaaaagt aaattttcgg ggtagagagt ggacagtgga agttttagac caaaatagct
    14701 tccggggagt ggacattgcg ctttttagcg ctggagcttc cataagccga gaattcgctc
    14761 ccttagcagc gcagagtggc tgtgtagtga tagataattc ctcagctttt cggcaagacc
    14821 ccgacgttcc tttaattgtc ccggagatta atcctcaccg gattaaagat tacaggcaaa
    14881 gaaatatcat tgctaaccct aactgtacta ccatcatttc tcttatgcct ttgaaacctt
    14941 tgcacgacta tgggaaaatt aaaagggtaa tagcggctag ctaccaagct acttcgggtg
    15001 caggagctaa agctatggct gagttggaag aacaaaccaa agcctatata agccaggaag
    15061 aaatagtgcc tcatgttttt ccccagcaaa ttgcttttaa cgttataccg catatcgatg
    15121 ttttcctgga taactactac actaaagaag agatgaaaat ggtctgggag agtcgaaaaa
    15181 tcctggaaga agaaaacctg ttaattagtg ctacttgtgt ccgagttcca gtgcttcgcg
    15241 cccatgccgt ggctattaat atagaaacag aacaaaagat aaccagggat aaagccatag
    15301 aacttttatc ctctttcccg ggggtgaaag tagtagatga tccagagcat ctactttatc
    15361 ctactccttt aatggctgca ggaaaagatg aatgtttagt aggaagaata agggaagaca
    15421 tttcctctcc tttgggctta aatctctggg tagtgggaga ccagttgaga aagggagcag
    15481 cactaaacgc cgtacaaatt gctgagcttt tagccccaga aatttcttaa ccattagggg
    15541 ttgcgagaaa acagaaatta ttttaaaatt gcaatgctgc gagagtggcg gaatggcaga
    15601 cgcgctggac ttaggatcca gtggcaatcg ccgtgcgggt tcaactcccg cctctcgcac
    15661 catatgttct aaattagacg ttttaaattc ttctaaaatt ctattttcca aaatatatga
    15721 ggtaaaattt gaaattttaa gtgat
//
LOCUS       DAOSVE010000030        15729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_2300_length_15729_cov_65.796032, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000030 DAOSVE010000000
VERSION     DAOSVE010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 15729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15729
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2300_length_15729_cov_65.796032"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /gene="rsxC"
                     /locus_tag="PK016_03955"
     CDS             <1..1146
                     /gene="rsxC"
                     /locus_tag="PK016_03955"
                     /inference="COORDINATES: protein motif:HMM:NF003454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HXL02257.1"
                     /translation="ADVEAFISAPLHSPLAGKVAGIEDYFHPLLGRFPALTIENDGSG
                     EEERKDPLLDIEGKNPTFLREWIREGGMVGLGGAAFPTHVKVSPPSGKKIDSFILNGA
                     ECEPYLTCDYRVMVERPTTVLTGLRVLVRACGAEKAFIGIEENKTEAIEKFSELVKDL
                     PFPTCVVPLLTKYPQGSEKQLIFSILGREVPPGGLPLDVGVVVNNVQTSWAIGRMAEE
                     GLPLIERVITLSGECLRNPENLQVPLGVILSDLVDFCGGWVETPKKVVMGGPMMGVAQ
                     TSLQVPVIKGTSGFIFLNHLYEKKEYPCIRCRRCIDACPMRLFPTMLGKFSEYQAFDE
                     AKENRVLDCMECGACSYVCPSNISLTHLIRLAKAEIMRRSRRGKANG"
     gene            1139..2155
                     /locus_tag="PK016_03960"
     CDS             1139..2155
                     /locus_tag="PK016_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HXL02258.1"
                     /translation="MDEELKLVITSSPHLRDETTVPWIMRQVVLALLPATIWGMCFFG
                     VSSTLLTILLSIGSTIGFEALNDKIFKKPLSITDGSAVVTGLLLGLSLPPSAPFWLPI
                     VGGGAAIFLGKEMFGGLGQNVFNPALVGRAVLLVSWPKVMSTWWVNTPFDFSTFAEGG
                     AASWGVRMVTTPTPLGVARIYGYSALHQADPRILWHLFLGQVPGSIGEISVLLLIIGF
                     LYLWWRGIVHWDIPLFYFIGLSLVAILGGQSAIFYILSGGAVMGACFMATDYVTSPIS
                     SKGRYLFGLGAGAITGVIRLLGAYPEGVTFGILTMNALVPFLDHLLPQRFGVKKSNPG
                     VRKL"
     gene            2152..2709
                     /locus_tag="PK016_03965"
     CDS             2152..2709
                     /locus_tag="PK016_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HXL02259.1"
                     /translation="MKNIWKVALPLAVFCVVAAFALAAVNSVTQKEIERRSQEGLEEA
                     LQKVAPFAQEFQKWETVPAVQASTKDFSILEIYEALKEGKREGLVFRVSTTGYGGPVI
                     LLVGISEEGKLTGMEVLEHQETPGLGSNIESTDFLSQFLGKSLTDSFEINKDIKAISG
                     ATISTRAIVRACQGLASYWEEIGKP"
     gene            2706..3305
                     /locus_tag="PK016_03970"
     CDS             2706..3305
                     /locus_tag="PK016_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HXL02260.1"
                     /translation="MKEFWSCFKNGIIGENPVLRLMIGLCSVLAVSVRLENCLYMGAA
                     VIFVLTGSNMVISAIRNFVPNEVRIPIFIVVISTFTTIVDLVMKAYLPALSEALGVFI
                     PLIVVNCIIMARAEAFASRKPVLLTMADSLGMGVGYTLVITAIGIIRELFGYGQIFYH
                     QIFPSSYQGMMIMILPPGAFLLIGLCIAILNHFYRRNNL"
     gene            3311..3886
                     /locus_tag="PK016_03975"
     CDS             3311..3886
                     /locus_tag="PK016_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit A"
                     /protein_id="HXL02261.1"
                     /translation="MDLFKIFISAAFVDNIILARYIGLCPYIGMSSNVANSLGMGMAV
                     TFVMVLASIATWVLWNFIFVPLGLEYLQIMVFILVIAVLVQLLEAILKKMVPNLYQAM
                     GIYLPLITTNCAILAVTFFGVDYHYNLIQVVVYSIGVSLGFTIALLLLAGIREKLRFS
                     KVPQFYQGYPMVFISTCLLAIAFMGFKGLVK"
     gene            3896..4741
                     /locus_tag="PK016_03980"
     CDS             3896..4741
                     /locus_tag="PK016_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HXL02262.1"
                     /translation="MNILMPTILIGVLGLVFGVMLAWFSQKFAVETNPLVQKAEKILP
                     GANCGACGFPGCSALAEKIAAGEAPPDACPVGGPKVWNELSSLLGKEIDSNREAQKAI
                     LLCQGEKEVAGEVATYQGVKDCRAAHLLGNSFKVCRYGCLGFGNCVKVCPFEAMILDE
                     EKGLPVILWEKCTGCGKCVEECPRSIIALLPAREQVVEACVSLASPRETRQSCKKGCI
                     KCSICVKSCPEGAMTLEGGRIKIDRDKCNLCGICIEKCPTKCLVWVEKKPSLEEKEVG
                     VRVGG"
     gene            4749..6221
                     /locus_tag="PK016_03985"
     CDS             4749..6221
                     /locus_tag="PK016_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HXL02263.1"
                     /translation="MLLGIDVGTTNTKLRVYDQDGEIKGEESFPTPLFPDEEGELYDP
                     QEILREILRAINRFPGEVKQEIVALSVSSYAEVLVKVGENGEPLGGSIPWYDNRAEKY
                     FEDVELDREEVYRISGLYPYHIYSFYKLLWHREREKETFEKTKFWTSMSGFILFYFSG
                     VPSFDYSLASRTMLFRQRERCWWEEMLERVGLSVKNMAPLYPSGRVLGTVREKVAREC
                     GLSPDTLVITGGHDHLCAALSVGVYKEGRALISTGTTESLTMALESLPSLSIEGEEKP
                     FSWGHHVAYPYYYAMNGIYSGGYSVDWALKLLKEDYSFLENCSFSLSSGVPLFFPYLL
                     GGDYEGARGAFFNLTGNTGREELILGLLLGLCFEYRNIWEKMEEKLGFKVKEAVNVGG
                     GTQNKIWMKLKSVTLGKNIFVPRDREGSCRGAALLAGLGAGVYKDFEEAFQKTFRVKE
                     MFKPQEESQKSLEKWFRTYVEVEKDLRIINKKLGVLYGRE"
     gene            6208..6462
                     /locus_tag="PK016_03990"
     CDS             6208..6462
                     /locus_tag="PK016_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HXL02264.1"
                     /translation="MAENRVGVVGIVVTNREKQAEQVNEILGEFGELIVGRMGIPYRE
                     RNISVIALIVDGSTDELGALTGRLGNIPGVKVKSALVSFS"
     gene            complement(6459..7655)
                     /locus_tag="PK016_03995"
     CDS             complement(6459..7655)
                     /locus_tag="PK016_03995"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL02265.1"
                     /translation="MRILYSRPKKRSVPNFFIFLSIASASNFIFALTSVLFSIFATDM
                     GFSPPEIGLAISALTAGSLIGGPLWGKLVDKFGKRKIALLVSLSGQALFCLLTPLMKH
                     LLSLVIVRFFFGFFTVAQALILNELTVKIEEEERRSKEISAINVARAAGYSSGCLLSG
                     ILGDLHPNFSFYLGSLAVILVFFLTFYLREETEAATPLPQDKNPTEHWFWQKGVVPFY
                     LSIFLRSTAVNGLSYFLPLFWKNMGQTTALIGSVIAISNLAQLLFFPLSGKVSSRSDL
                     MAFRSARFGLLLTIIPFLIAPFFHQGVLILLPQCILSISWAFFYIGTILSLRSFVPPK
                     RQGEALGWLEVAFNLGGIVGPNLFAFSLSSFHNNFLLAFFSLSFLPLVAFFISRKNEK
                     RLLSRN"
     gene            complement(7639..8772)
                     /locus_tag="PK016_04000"
     CDS             complement(7639..8772)
                     /locus_tag="PK016_04000"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL02266.1"
                     /translation="MALFLFHFLADFFASFPSPLSPYFIELYKVDSRTIAMFIATLSL
                     LGSLSQLFFATRLLFQKPEIQFYISLSCLMLPWLFIPFSSHIYTLFFILIVAFLANAS
                     FHPLGAALSGELGPQGVTFFVSGGIVGGALGPIFITLLVSKAGASGIALLTLGLWACW
                     TVLFLRKRDYHIPSFSPPEKFSLRLFPPLLPIWIMVSVRTFFTDIFHTFVPIYLSLQG
                     YSLVVGGTLLSAGVMSGLLANLVGSRIRIQFNNRLVNLISFGGLGICILTFTWVEEIT
                     FLAIFYLLADFCSFLSMSTNVCEAQDLLPQHRALASSVAMGLSWAFGHFLHLLYSSLL
                     GREVILVLQSAGLFSIGVVILLLLFPSFFQRKTYGVERVENII"
     gene            9197..10318
                     /locus_tag="PK016_04005"
     CDS             9197..10318
                     /locus_tag="PK016_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL02267.1"
                     /translation="MRRLISLSTVLLLVLSFSGVVLAQETVKIGANLEITGGVAAYGQ
                     MIWEGVNVAKEVVGDEVLGKKVELVLVDNKSDRVESANAATRLIDYEKVVVMVGPAIS
                     GSMLAVGPICEEKQVPVISASATSPLVTQGKKFVFRACFRDDDQAKAAAQFARSELQA
                     QTAAILSDIAQDYCVALGNFFKDAFIERGGQVVSEQNCRSGDQDFSAQLTTIINANPD
                     VIYVPNYYTETALICQQARDLGYEGMILGADGADAPELVSIGGDSVEGVYFTAHADTT
                     QAITEIGQKFFDLYREKHGREPSAFEALGGDCYVIILDAIKRAGELDPVKIRDAMEDT
                     ENLEVITGVATIVDGDNKKPIVVKRVENGNFNFVTTIEY"
     gene            10373..11266
                     /locus_tag="PK016_04010"
     CDS             10373..11266
                     /locus_tag="PK016_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXL02268.1"
                     /translation="MTWVSFFQQIMNGVSLGSLYALLAIGYTLVYGILGFINFAHGDV
                     FMLGAYFSFFMIGVFLLPWWLGVIIGVALCAIVGMSIERVAYRPLRNAPRISALITAV
                     GVSFFIENLGLVTVGARPKGFPRPELMKNVYILAGARIQGVTFWVPIISILVLLVLLY
                     LVYRTKMGMAMRAVSKDMETTRLMGVDVDRIILYTFALGSALAGIGGVLWACKYPQIN
                     PLMGTFPGWKAFTAAVVGGIGNVVGAMIGGFIIGLVEIMIVAFFPGLSGYRDAFVFSI
                     LIIFLLVKPSGILGESLKEKV"
     gene            11269..12330
                     /locus_tag="PK016_04015"
     CDS             11269..12330
                     /locus_tag="PK016_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXL02269.1"
                     /translation="MGKEEKRDLILTLLLFAVLMWGVSLAGRYLNTYLIRILNTACIY
                     VVAAVAYNLINGITGQFSLGPNGFMAIGGYTVAILMLPLVQKQQVYFLQPLIWPFNSF
                     SFPSSLFILALFLGGVAAALAALLIGIPTLRLRGDYLAIATFGFGEIINVLANNFIPL
                     TNGALGIKGIPEYTNLWWTVNWAFWSVFMIKGLANSSFGRALKAIRENEVAAEAMGVN
                     LFRHKLLSFVLSGFFAGVAGGLFVTLISTVSPSLFTFTMTFNLLIIIVLGGLGSITGS
                     VITAFIFAFLQEILRVVEAPITIGAWTFPGIPGMRMVVFSVLLVVMMIFYRQGLFGNK
                     EISWQLIIDWIRGRNESGK"
     gene            12314..13096
                     /locus_tag="PK016_04020"
     CDS             12314..13096
                     /locus_tag="PK016_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL02270.1"
                     /translation="MKVENKEPLLSLEDLTIAFGGLVAVSDFSLQLFPGDLVGLIGPN
                     GAGKTTVFNLITGQYRPTKGRIIFQGKDITGFSPDVVTTVGIARTFQNIRLFGNLTVL
                     ENVLVSFHVRLQSSFLAPMFSLPSYWREERAIREEAYELLKAVGLADATKEEAQSLPY
                     GEQRRLEIARALATHPSLLLLDEPAAGMNPQETQELMRFIQKIRDDFNLSIFLIEHDM
                     KVVMGICERIAVMDFGMKIAEGTPEEIRNNPRVIEAYLGVEN"
     gene            13096..13818
                     /locus_tag="PK016_04025"
     CDS             13096..13818
                     /locus_tag="PK016_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL02271.1"
                     /translation="MEEDLILKTEDLHVFYGGIHALKGINIKVPVGKIVALVGANGAG
                     KSTTLRTISGLVRARQGSIEFLNRDITREASHHIVEMGLAMAPEGRRIFPNLTVWENL
                     LLGSYTRLDREEIKKDLDWVFQIFPRLKERVRQKGGTLSGGEQQMLAVARALMSRPHL
                     LMMDEPSLGLAPLLVKEIFNIVQEINRNGTTILLIEQNAKAALQVADYAYVLETGSVV
                     LEGMGKELLENEKVRKAYLGEE"
     gene            13822..14025
                     /locus_tag="PK016_04030"
     CDS             13822..14025
                     /locus_tag="PK016_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02272.1"
                     /translation="MATGREEYRDKGIQEVYDTPYEKKLKKWFWIKDKRIKGRYLHPR
                     EESKIMDELVCLRCGKKCRGECQ"
     gene            complement(14058..14696)
                     /locus_tag="PK016_04035"
     CDS             complement(14058..14696)
                     /locus_tag="PK016_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HXL02273.1"
                     /translation="MEKEGAKRILCFGDSLTWGADPETGGRHPVSSRWTTLLQKNLGE
                     NYWIIEEGLGGRTTIWDDPIEGDKNGRRHLPILLESHRPLDLVVIMLGTNDLKSRFHV
                     SAFEIAQSASTLVEVVKRSSAGRGGNPPLVLLIAPPPLGRLTIWQEEFGEGVEKSRNL
                     ARFYSYFAQLNQCHFFDAGEVVKTSDIDGIHWEANANRQFAEALAEKIKGIL"
     gene            complement(14918..15466)
                     /locus_tag="PK016_04040"
     CDS             complement(14918..15466)
                     /locus_tag="PK016_04040"
                     /inference="COORDINATES: protein motif:HMM:NF039195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsbP-related protein"
                     /protein_id="HXL02274.1"
                     /translation="MKRIYFILALSILIIGFLFVYFVYFTQENSATYTYTSSAGFSLT
                     FPPDWEVNNSPSTLNPYLMMLGQDSEAQVEVTLEYYNYSSLEDFFEKVKEEVSQFPGV
                     KIISQGEESINGAPSYWFTYSFLASIGPSDPQEPFQAILYLFSQGGNFFRIICLTKED
                     RFNDYLNTFQTIARSFTTLTEN"
BASE COUNT         4537 a         2688 c         3747 g         4757 t
ORIGIN      
        1 gctgatgtag aagcttttat ttctgcccct ctgcattctc cattggctgg taaagtagcc
       61 gggatagaag attatttcca tcctctactg gggaggtttc ctgccctaac catagaaaat
      121 gatggttcag gggaagaaga aaggaaagat cctctcctcg atattgaggg taaaaacccc
      181 actttcctcc gggaatggat tagagaaggg ggaatggtag gcttaggagg agctgctttt
      241 cctactcatg ttaaagtttc tcccccttca ggtaaaaaaa tcgatagttt tattttaaat
      301 ggggcagaat gcgaacctta cctgacttgt gattaccggg tgatggtaga aagaccaact
      361 actgttttga ctggcttgcg agttttggtt agggcttgtg gagcagagaa agctttcata
      421 gggatagagg aaaataaaac tgaagctata gagaagttta gcgagttagt aaaagacctc
      481 cccttcccta cttgtgtagt tcctcttctt actaaatatc ctcagggttc agagaaacag
      541 ttgatttttt ccattttggg aagagaagtt cctccgggag gattgcccct cgatgtggga
      601 gtggtggtaa ataatgttca aactagctgg gcaataggaa gaatggctga ggagggtctt
      661 cctctcatag agcgagttat aactttaagt ggagaatgtc ttcgaaatcc agaaaacctt
      721 caagtacctt taggagttat tttatcagac cttgtggatt tttgtggagg atgggtggaa
      781 acgcccaaga aagtggttat gggaggacca atgatgggag tggctcaaac ctccctgcaa
      841 gtaccagtga taaaaggaac ttctggtttt atatttttga accatcttta tgaaaagaag
      901 gaatatcctt gcattcgttg ccgcagatgc atagatgcct gccccatgcg tctcttcccc
      961 accatgctgg gtaaattttc tgaatatcag gcttttgacg aagctaagga aaatcgggtt
     1021 ttagattgta tggaatgtgg agcctgttct tacgtttgcc cttccaatat ttctcttact
     1081 catctcatca ggttagctaa agcggaaatt atgcgccgtt ccagaagggg gaaagcaaat
     1141 ggatgaagaa ttaaaattgg ttattacttc ttcgcctcat cttcgagatg aaactactgt
     1201 tccttggata atgagacaag tagtgctggc tcttttacct gctactattt ggggaatgtg
     1261 cttttttgga gtttcttcta ctctcttgac cattctttta tctataggga gcacgatagg
     1321 atttgaggct ctcaatgata agatttttaa aaaacctctc tctattaccg atggaagtgc
     1381 agtagttacg ggcttgcttt tgggtttatc tcttcctcct tctgcgcctt tctggctacc
     1441 tatagtggga ggaggagcgg ctattttcct ggggaaagaa atgtttggag gtttgggaca
     1501 aaatgtattt aacccggctt tagtagggag agcagtttta ttggtttcct ggcccaaggt
     1561 tatgtctacc tggtgggtta atactccttt cgatttttct acttttgctg agggcggtgc
     1621 tgcttcctgg ggagtaagga tggttactac tcctactcct ttgggtgttg caaggatata
     1681 tggctatagt gcattacatc aagcagaccc tcgtattttg tggcatcttt ttttagggca
     1741 agttcctggt tctattggag agatttcggt gctactactc atcattgggt ttttgtatct
     1801 gtggtggcgg gggattgtgc attgggatat ccctcttttt tatttcattg gtctctcttt
     1861 ggtagctatt ctgggaggtc agagtgctat cttttatatc ttaagcgggg gggcagttat
     1921 gggagcatgc tttatggcca cagattatgt gacttctccc attagctcaa agggacgtta
     1981 tctttttggt ctgggagctg gagctatcac aggagtaatc cgtcttttgg gggcctatcc
     2041 tgagggagtt acctttggca tccttactat gaatgcctta gttccttttt tagatcatct
     2101 gcttccccag aggtttgggg taaaaaaatc aaatccgggg gtaagaaaat tatgaagaat
     2161 atctggaaag ttgctcttcc tttagctgtg ttttgtgttg tagctgcttt tgctttagca
     2221 gcagtaaatt ctgtcaccca aaaagaaatt gaaagacggt cgcaagaagg cttagaagaa
     2281 gctttacaaa aagttgctcc ttttgctcaa gagttccaaa aatgggagac tgtgcctgcg
     2341 gtgcaagctt ctactaaaga tttttccatt ttagaaattt atgaggcgtt aaaagaagga
     2401 aaaagagaag gattggtgtt tcgagtgagc accaccgggt atggcggacc agttatactc
     2461 ttagtgggaa tttctgagga agggaagctc acggggatgg aagtattaga acaccaagaa
     2521 accccgggac tgggctctaa tattgaaagt actgactttc tatctcaatt tcttggtaaa
     2581 tccttaaccg actcctttga aattaacaaa gatattaaag ccataagcgg ggctactatt
     2641 tccactcgag ctatagtccg ggcttgccag ggtttagcaa gctattggga ggagatagga
     2701 aaaccatgaa agaattctgg agttgtttta aaaatggtat tataggagaa aacccggttt
     2761 tgcgcttgat gattggtttg tgttcggtat tagcggtgtc agtgaggtta gaaaactgtc
     2821 tctatatggg agcagcagtt atttttgttt taactggttc taacatggta atatcggcga
     2881 taaggaattt tgtccccaat gaagttcgta ttcccatatt cattgtagtg atttctactt
     2941 ttactaccat tgtagatttg gtgatgaaag cttatcttcc tgctctttct gaagccttgg
     3001 gagttttcat ccctctgatt gtagttaatt gtattattat ggctcgagca gaagcttttg
     3061 cttctcgaaa accagtgctg ctcacaatgg ctgattctct ggggatggga gtaggctaca
     3121 ctctagtaat cacagccatt ggtataataa gagaactttt tggttacgga caaatttttt
     3181 accaccagat atttccttct tcttaccaag ggatgatgat aatgattctt cctcctggag
     3241 cttttctcct tattggcctt tgcattgcta tactaaatca cttttatcgg aggaataacc
     3301 tatgaatgct atggaccttt tcaaaatttt tatcagtgct gcttttgtgg ataatattat
     3361 tttggctcgc tacattgggt tgtgtcccta tatcggaatg tcttctaatg tagcaaactc
     3421 tttagggatg gggatggcag ttacttttgt catggtttta gcttctatag ctacctgggt
     3481 tttgtggaac tttatttttg ttcctttagg gttggaatat ctccagatta tggtgtttat
     3541 tctggtaatt gccgttttag tgcaattgtt ggaagcaatc ctcaagaaaa tggtgcctaa
     3601 cttataccag gctatgggca tataccttcc tctcatcact actaattgtg ctattttggc
     3661 ggttaccttt tttggagtag attatcatta taaccttatt caggtggtag tctatagtat
     3721 aggggtgtct ttggggttta cgatagctct gttacttctg gctggaatca gggaaaaatt
     3781 gagattttct aaagtccctc aattttacca gggttatcct atggtattta tctctacctg
     3841 tcttttagct attgccttta tgggttttaa gggtctagtg aaataggaga aagatatgaa
     3901 cattctaatg cctactattc ttattggagt tttaggtttg gtttttggag tgatgttggc
     3961 ttggttttct caaaaatttg cagtagaaac taacccttta gtgcaaaaag cggaaaagat
     4021 tcttcctgga gctaattgtg gagcttgtgg ttttccagga tgttctgctt tagcagagaa
     4081 aattgctgct ggagaggctc ctcctgatgc ttgtccggta ggagggccaa aggtgtggaa
     4141 tgagctatct tctttattag gaaaagaaat tgactctaac cgggaggcac agaaagctat
     4201 tctcctttgt caaggggaga aagaagtggc aggagaggta gcaacctacc agggagtaaa
     4261 agattgcaga gctgctcatc ttttgggcaa ttcctttaaa gtctgccgct atggatgcct
     4321 gggtttcgga aattgcgtga aagtttgccc ttttgaggct atgattttag atgaagaaaa
     4381 aggattacct gtgattctct gggagaaatg tactggttgc ggaaaatgcg tggaagagtg
     4441 tcctcgctct ataattgctc ttcttcctgc ccgggaacaa gtggtagaag cttgtgtttc
     4501 tttagcttcc cctcgggaga ccaggcaatc ctgtaaaaag ggatgtataa aatgcagtat
     4561 atgtgtgaaa tcctgcccgg agggagctat gactctcgag ggaggaagga taaagataga
     4621 ccgggataag tgtaatttgt gtggaatttg tatagagaaa tgtcctacta agtgtttggt
     4681 atgggtagag aaaaagccct ctttagaaga aaaagaggta ggagtgaggg tgggaggata
     4741 aactggctgt gcttttaggg atagatgtag gaaccactaa tactaagtta cgggtttatg
     4801 accaggatgg agaaataaag ggggaggaaa gttttcctac ccctttattt cctgatgaag
     4861 aaggagaact ttacgatccg caggagattt tacgagagat tctccgggct ataaatcgat
     4921 ttcccgggga agtgaaacaa gaaattgtag ctctcagtgt atccagctat gcggaggtac
     4981 tggttaaagt aggtgagaat ggagaaccac tgggaggaag tataccctgg tatgacaatc
     5041 gcgccgaaaa gtattttgaa gatgtagagt tagacaggga agaagtttat cgaattagtg
     5101 gcctttatcc ttatcatatt tattcttttt ataaattgct ctggcaccgg gaaagagaaa
     5161 aagaaacttt tgagaaaacg aaattctgga cctctatgtc tggttttatt ctcttctact
     5221 ttagcggagt tccttctttt gattattctt tggcttctcg tactatgctt ttccgccaaa
     5281 gagagaggtg ctggtgggag gaaatgttag agcgagtggg attatcagtg aaaaatatgg
     5341 cacctcttta tccctctggg agagttttag gaacagtaag agagaaggta gcaagagagt
     5401 gtggtttatc ccctgatacg ctggtaataa ccggagggca tgaccatctc tgtgctgctc
     5461 tttcagtggg agtatacaaa gaagggagag ctttaatctc taccggaact actgaatctt
     5521 tgaccatggc actggaaagt ttgccttctc tctccataga gggggaagaa aagccttttt
     5581 cttgggggca tcatgtagct tatccttatt attatgctat gaatgggatt tatagtggag
     5641 gctatagtgt tgattgggca ctaaaattgt taaaagaaga ctatagtttt ctggaaaatt
     5701 gctctttttc tctctcttcc ggtgttcctc ttttttttcc ttatctttta ggaggagatt
     5761 atgagggagc aaggggagca ttttttaacc tcacgggaaa cacaggaaga gaagagttaa
     5821 tcctgggtct tcttttaggt ctgtgttttg aatatcgaaa tatctgggag aaaatggagg
     5881 agaagttagg atttaaggta aaagaagcgg ttaatgtggg agggggaact caaaataaga
     5941 tatggatgaa gttaaaatct gttactctgg gcaaaaatat ctttgttccc cgggatagag
     6001 aaggaagttg ccggggggct gctcttctgg ctggtttagg tgctggagtt tataaagatt
     6061 ttgaggaggc attccaaaaa accttccggg ttaaggaaat gtttaagcct caagaggaga
     6121 gtcaaaaaag tttagagaag tggtttagaa cttatgtgga ggtagagaaa gatttgagga
     6181 taattaataa aaagttaggg gttctctatg gcagagaata gagtaggggt agtgggaata
     6241 gtggttacca atcgagaaaa acaagctgaa caggtgaacg aaattctggg ggagtttgga
     6301 gagctaatcg tagggagaat gggtatccct tatcgagaga gaaacatttc ggtaattgct
     6361 ttaattgtag atggtagcac agacgagctg ggggctttaa ctggtagatt gggcaacatt
     6421 ccgggagtaa aagtaaagtc agctctggtt tctttttctt aatttcgaga aagaagtctc
     6481 ttctcgtttt tcctggaaat gaagaaagct acaaggggca aaaaagaaag ggaaaagaag
     6541 gcaagtagaa agttattgtg aaaagaagaa agagaaaagg caaaaagatt aggacctact
     6601 atacctccta aattgaaggc tacttctagc catcctaaag cttctccctg tctctttggg
     6661 ggaacgaaag aacgaagaga aagaatagtc ccgatgtaaa agaaagccca gctaatgctc
     6721 aaaatacatt gaggaagaag gattaatact ccttgatgaa aaaaaggagc aatgagaaaa
     6781 ggaataatag ttagcaaaag cccgaaacgg gcagaccgaa aagccatcag atctgaccgg
     6841 gagcttactt tcccacttaa agggaagaaa agtaattggg ctaaattaga gatagcaatg
     6901 accgagccaa tgagagcagt agtttgcccc atatttttcc agaaaagggg caagaaataa
     6961 gaaagtccat ttactgcggt agagcgaaga aagatggaaa gatagaaagg cacaactcct
     7021 ttttgccaaa accagtgttc agtaggattt ttatcttgag gcaagggggt agctgcttcg
     7081 gtttcttccc ggagatagaa agtaagaaaa aaaaccagaa taacagctaa acttcctaaa
     7141 tagaaactga agttagggtg gaggtctcct aaaattccgc tcaaaagaca acctgaagag
     7201 taacctgctg ctcgagcgac attgatggct gatatttctt tgcttcttct ttcttcctct
     7261 tctatcttga ctgtcaactc attaagtatt aaagcctggg ctaccgtgaa aaatccaaag
     7321 aaaaaccgta ctattactaa acttaagaga tgcttcatca ggggagtaag caagcaaaaa
     7381 agagcctgtc cggaaaggct gactaaaaga gctatttttc tcttgccaaa tttgtctact
     7441 aattttcccc ataaagggcc acctattaaa gatccagcgg tgagagcaga tatggctaaa
     7501 ccaatttccg gaggtgaaaa tcccatgtct gtggcaaaga tagaaaagag aaccgaagtt
     7561 aaggcaaaaa taaaattaga agcggaagca atacttaaga atataaaaaa gtttgggacg
     7621 ctcctttttt tgggccggct atataatatt ctcaactctc tcgaccccgt aagtttttct
     7681 ctggaaaaaa gaaggaaaaa ggagaagaag gataactact cctatagaaa agaggccagc
     7741 actctgaagc accaagatta cctctctacc cagtaaagaa gagtagagaa gatgtaaaaa
     7801 atgtccaaaa gcccatgata ggcccatggc taccgaagag gcaagagcac gatgctgagg
     7861 aagcaaatct tgagcttcac aaacattggt gctcatactc aaaaaagagc aaaaatcggc
     7921 taagagataa aaaatggcca aaaaagttat ttcttccacc caggtgaaag ttaaaatgca
     7981 aatacctaat cctccaaaag agattagatt taccaatcga ttattgaact gtattctaat
     8041 tctgcttcct actaagttag ctaaaagccc ggacattact cctgcggaga gtaatgttcc
     8101 ccctactact aagctatatc cttgtaagct caggtagata ggaacaaagg tatggaaaat
     8161 atcggtgaaa aaagtgcgga cactcaccat tatccaaata ggtaacaaag gaggaaaaag
     8221 tcgaagggag aatttctctg gaggactaaa ggaaggtata tggtaatccc tttttctaag
     8281 gaataaaaca gtccagcatg cccaaagtcc taaagttagc agtgctattc cagaagcacc
     8341 tgccttgctt accagcaaag taatgaaaat agggcctaga gcgcctccga caatgcctcc
     8401 agaaacaaag aaagttactc cttgaggtcc aagctcacca gagagagctg ctcctaaggg
     8461 atggaaagaa gcattggcta aaaaagctac tataaggata aagaaaagcg tataaatatg
     8521 agaagaaaaa gggataaaga gccagggtaa cattaaacag gaaagggaaa tatagaactg
     8581 tatctccggt ttctgaaata aaagtcgggt agcgaagaaa agctggctaa gagaacccag
     8641 aagagagagg gtggcaatga acatggctat ggttcgagaa tctactttgt ataactctat
     8701 aaaataagga ctaagcggtg agggaaaaga agcaaagaaa tcagctaaga aatgaaaaag
     8761 aaaaagcgcc aaattttatt tttcctctct ggtgttagtt atttattcct atgaacctga
     8821 taaatttaca caattttatc acggtaaaaa ttaaactcaa gatattgagc tgcctcagtt
     8881 acaattaaag ctaaattgtt atttctttaa aaggatgttc ccaagagggt tacaagtgag
     8941 gtatctcttg caaacacaaa aagagattat ttatgagata agtaaaggac ataagaagct
     9001 atccaaaaaa gatagaaact ttttaatctg ataaccatta ctggttacaa tcgtgattat
     9061 gttgataatt actattttga taaattttct tgtcttctat actccgatag agcccggttt
     9121 ttcttgacaa ggagctggtg cagtgataat tttatggcaa gttttaacca aaaaatagcg
     9181 aaagggggat tttagattga gacgattaat tagtttatcc acagttcttc ttttagtttt
     9241 gagcttttcc ggggtagttc tagctcaaga aacagtgaaa attggagcta acctggaaat
     9301 tactggagga gtagcagctt atgggcagat gatctgggaa ggagtaaatg ttgctaaaga
     9361 agtagtagga gatgaagttc tggggaaaaa ggtagagctg gttttagtag ataataagag
     9421 tgaccgggta gagtcagcta atgctgccac tcgcctgata gattacgaaa aagtggtagt
     9481 tatggttggc ccggcaataa gtggtagtat gttagcggta ggcccaatat gtgaagaaaa
     9541 acaggttccg gttatttctg ccagtgctac cagtcctctg gtcactcaag ggaaaaaatt
     9601 tgtattccgg gcttgtttcc gggatgatga ccaggctaaa gcggctgctc aatttgctcg
     9661 cagtgagctt caagcccaaa ctgcggccat tttatctgat atcgctcaag actattgtgt
     9721 agctctgggc aacttcttta aggatgcttt tatagaaaga ggagggcaag tggtaagcga
     9781 gcaaaattgt agaagtggag accaggactt ttctgctcag cttactacca ttataaacgc
     9841 taatcctgat gtcatttatg ttcccaatta ctacactgaa actgctctga tttgtcagca
     9901 ggcccgggat ttaggttatg aagggatgat tttaggggct gatggagcag acgcaccgga
     9961 attggttagc atcggtggag attcagtaga gggggtttat ttcactgctc atgctgacac
    10021 tactcaggcg ataactgaaa taggacagaa gttctttgat ttgtaccggg aaaaacatgg
    10081 tagagaaccc agcgcttttg aagctttggg tggtgattgc tatgttatta ttttagatgc
    10141 cattaaaaga gcgggagaat tagacccggt taaaatacgt gatgctatgg aagatactga
    10201 aaatctggaa gttataactg gagtggctac tattgtagat ggagacaaca agaaacctat
    10261 agtggtaaaa agagtggaaa atggtaactt caattttgtg actactattg aatattgaat
    10321 tggcagcacc aggcaaccgg gaaagttgcc tggtgctgta taaactaatg cgatgacctg
    10381 ggtttccttt tttcagcaaa taatgaatgg agtttcctta gggagtttat atgctctttt
    10441 agccattggt tatactctgg tttatggcat tttgggattt ataaactttg ctcatggcga
    10501 tgtattcatg ctgggagctt atttttcttt ttttatgata ggggtttttc ttctcccctg
    10561 gtggttggga gtgataatag gggtagctct gtgtgctata gtgggaatga gcattgaacg
    10621 ggtagcttat cgaccgctac gcaatgctcc tcgtatttct gctcttataa ctgcagtggg
    10681 agtttctttc tttattgaaa atctgggtct agtaactgtt ggtgctcgtc ctaaaggttt
    10741 tcctcgtccg gagcttatga aaaatgttta tattttagct ggtgctcgta ttcagggagt
    10801 aactttctgg gtgcctatca tcagtatttt agttcttctt gttttacttt atctagttta
    10861 ccgaactaaa atgggaatgg ctatgcgagc tgtatctaag gatatggaaa ccactcgtct
    10921 tatgggagta gatgtagata ggataattct ttatactttt gctttaggtt cggcattagc
    10981 cgggattggt ggagtacttt gggcttgtaa atacccccag attaatcctt taatgggcac
    11041 attcccgggc tggaaagctt ttactgcagc ggtagtagga gggattggta atgtagtagg
    11101 ggctatgata ggtggtttta taattggatt ggtagaaatt atgattgtgg cctttttccc
    11161 tggtttgtct ggctatcgag acgcctttgt tttcagcata ttaatcatat ttttgttggt
    11221 aaagcccagt ggaattctgg gtgaatccct gaaagaaaaa gtatagagat gggaaaagaa
    11281 gaaaaaagag acttaatttt aactttactt ctctttgcag tcttgatgtg gggagtaagt
    11341 ttagcgggaa ggtatctaaa tacttattta atccgcattt tgaataccgc ctgtatttac
    11401 gtagtagcag cagtagccta taatttaatt aatggaatta ccgggcagtt ttccttgggg
    11461 cccaatggtt ttatggctat tggcggttat actgtagcta ttttgatgct tcctctggtg
    11521 caaaaacaac aggtttattt cttgcagcct ctcatctggc cttttaattc tttttctttt
    11581 ccttcttctc tttttattct ggctctcttt ttaggaggag tagctgccgc tcttgctgct
    11641 ttattaattg gtattcctac tctccgtttg cgaggtgatt acttggctat tgctactttt
    11701 ggttttggag agatcattaa tgttctggcc aataatttta ttcctttgac taacggagct
    11761 ttaggaatca aaggaatacc ggaatacact aatctgtggt ggacggttaa ctgggctttc
    11821 tggtcggtat ttatgattaa gggtcttgcg aattccagtt ttggtcgagc tttaaaagct
    11881 atcagggaaa atgaagtagc agcagaagct atgggagtta accttttccg ccataagctt
    11941 ctgtcttttg tattaagcgg attttttgcc ggcgtggcag gagggttatt tgtaacttta
    12001 atcagcactg tttctccctc tctttttact tttactatga ctttcaattt attaattatc
    12061 attgttctgg ggggattggg tagcattacg gggtcagtaa ttactgcttt tatttttgcc
    12121 ttcttgcaag aaattttacg ggtagtggaa gctcccatca ctatcggagc atggactttt
    12181 cctggaatac caggaatgag aatggtcgtg ttttctgttc ttttagtagt catgatgatt
    12241 ttttatcgac agggattatt tggtaataaa gaaatttcct ggcagcttat tattgactgg
    12301 ataagaggaa gaaatgaaag tggaaaataa agagcccctt ctttctttgg aagatcttac
    12361 tatagctttt ggcgggctgg tagcagttag tgacttttct ttgcaacttt tccctggaga
    12421 cctggtgggt ttaattggac ctaatggagc aggtaagact acggtattta acttgattac
    12481 cggtcaatat cgtcctacga aaggacggat aattttccag ggaaaagata ttaccgggtt
    12541 ttctcccgat gtggttacca ctgttggtat tgctcgtact tttcagaaca ttcgtctctt
    12601 tggaaattta accgttttgg aaaatgttct ggtttctttt catgttcgct tgcagagctc
    12661 ttttttagct cctatgttca gtctcccttc ttattggaga gaagaaagag ccataagaga
    12721 agaggcttat gagcttctta aggcagtggg attagcagat gcaacaaaag aagaagccca
    12781 gtccttaccc tatggggagc aaaggcgttt agagattgcc cgagctttag ctactcatcc
    12841 ttctctttta cttttagatg agccagcagc gggaatgaat cctcaagaaa ctcaagaact
    12901 gatgagattt atccagaaaa tacgagacga ctttaatctt tccatatttc tgatcgaaca
    12961 tgacatgaag gtagtgatgg gaatttgtga gcggatagca gtaatggatt ttggaatgaa
    13021 aatagctgaa ggtacaccag aagagataag aaataaccca cgggttatcg aggcttatct
    13081 gggggtagag aattgatgga ggaagactta atacttaaga ccgaagatct tcatgtgttt
    13141 tatggtggta ttcatgctct caaagggatt aatattaagg ttccggtagg gaaaattgta
    13201 gctttggtgg gagcaaatgg agcaggtaaa agcacgactt taagaactat atcgggtctc
    13261 gtgagagctc ggcagggtag catagagttc ctgaatagag atattactcg agaagcttct
    13321 catcatattg tggaaatggg actggctatg gctccggaag ggaggagaat ttttcctaat
    13381 cttactgtct gggaaaatct gcttctggga agttacaccc ggctagatag agaggaaatt
    13441 aaaaaagatt tagattgggt tttccaaatc tttccccgtt tgaaggagag ggtaagacaa
    13501 aaaggaggta ctttaagcgg aggagagcaa caaatgcttg cggtagcgag agccttaatg
    13561 tctcggcctc atcttttgat gatggatgag ccttcgctgg ggttagctcc acttctcgtg
    13621 aaagaaattt ttaatattgt tcaggaaatt aaccgtaatg gaaccactat tctgctaata
    13681 gaacagaatg ctaaagctgc tttgcaggtg gctgattatg cctatgttct ggaaaccggg
    13741 tctgtggttc tcgagggaat gggtaaagaa cttctggaaa atgagaaagt acgtaaagcc
    13801 tatctcgggg aggaataaaa tatggccaca ggaagagaag aatatcgaga taaaggaatc
    13861 caggaagttt acgatacccc ttatgaaaag aaattaaaaa aatggttctg gataaaagat
    13921 aaaagaataa agggacgcta ccttcacccg agggaggaaa gcaaaataat ggatgagtta
    13981 gtgtgtctgc gatgcggtaa aaaatgccga ggtgagtgcc aatagtaaag caaaccttag
    14041 aaaggcctga tttttcctca gagaatacct tttatttttt ctgccagtgc ttcagcaaat
    14101 tgccgatttg cgttagcttc ccagtgaatg ccatctatat cgctggtttt aactacttcg
    14161 ccagcatcaa aaaagtggca ttggttaagc tgggcaaaat aagaatagaa acgagctaaa
    14221 ttcctggatt tttctactcc ctctccaaat tcctcctgcc atattgtaag tctccctaaa
    14281 ggtggaggag caataagaag aaccagagga ggatttccac ctcttccggc actacttctt
    14341 tttactactt ctaccagagt gctggcgctt tgagcaatct caaaggcaga aacatgaaag
    14401 cgagatttta aatcattggt tcccagcatt ataactacca aatccagagg tcggtgagat
    14461 tctaataata ttggtaaatg tcttcttcca tttttatctc cttcaatggg gtcatcccag
    14521 atggtagtgc gtccacctag tccttcttct attatccaat agttttctcc taaattcttt
    14581 tgcagtagag tggtccagcg agaactcact ggatggcgac ccccggtttc aggatcagca
    14641 ccccaggtca gggaatctcc aaaacagagt atgcgttttg ccccttcttt ttccattttt
    14701 tcacctctta gcttggtgtt attttctttt ataaaatggt gttatagtta accgtaaaat
    14761 tcataataat atttttctat ttaatgctcc atcttctcaa gagataatca tgcgacaatc
    14821 cttactaaac cgtcgttgcg aacctcgtgt gctcgcctgg catggcaatc tctccttttc
    14881 agagagaaat tataacaggt tttgcaagat actataatta attttctgtt aaggtcgtga
    14941 aactacgagc aatggtttga aaagtgttta aataatcatt gaatcgatct tctttagtta
    15001 gacaaattat acggaaaaaa ttgcccccct gggagaaaag atagagaata gcctggaagg
    15061 gttcttgagg gtcggaaggt cctatcgaag ctagaaaact ataagtgaac cagtaactgg
    15121 gagctccatt tatagactct tccccttggg aaattatttt cactccggga aattgactta
    15181 cttcttcctt cactttttca aagaaatctt ccagtgaaga ataattataa tactccaaag
    15241 taacctcgac ctgggcttcc gagtcttggc ctaacatcat aaggtaggga ttgagggtag
    15301 aaggagaatt attgacctcc caatcagggg gaaaagtgag ggaaaatcct gctgaagagg
    15361 tataagtata ggtcgctgaa ttttcttgag taaaatatac aaaatatacg aaaagaaaac
    15421 ctataattaa tatgctcaaa gctaagataa agtaaatcct cttcactttt tcctcctcga
    15481 taacttttca ctattatatc atcttgctat caggaatccg aactatttaa ggacaaaaac
    15541 atttagatac ctgataaagg atttcaggca tgacggaggg gaagctcttg aattatccct
    15601 tcttttcttc tccctttaag ggttagggtg aaggtgattt cccttctgtc attttataag
    15661 gccaattaca ttgtgttaaa aactcgggat cgccacgcac ggtaagatac gccgtgctcg
    15721 cgatgacgg
//
LOCUS       DAOSVE010000031        15633 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_2314_length_15633_cov_67.940108, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000031 DAOSVE010000000
VERSION     DAOSVE010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 15633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15633
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2314_length_15633_cov_67.940108"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2867
                     /locus_tag="PK016_04045"
     CDS             <1..2867
                     /locus_tag="PK016_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02275.1"
                     /translation="CNEKAKEEGMLCWLYDEDRWPSGFAGGYVTKDNRYREHFLVFTP
                     FPYENMEEEISHLLPEGKTVKGKNRKLLAKYEVVLENGYLVYYQRLKEETGKEGKIWY
                     AYSEISADDPWFNNQAYVDTLNRKAIEKFIEITHQRYYEILGEDFGKSIPAIFTDEPQ
                     FVRKDCLSFSEEEREVILPFTDDLEESYYKVYHQSLLDHLPELIWELPDNKASLVRYR
                     YHDHVTERFVQAFADTIGEWCEKHKIMLTGHMMEEPTLESQTAAIGEAMRPLRSFGLP
                     GIDMLCDRREFSTAKQAQSVTHQYGREGVLSEIYGVTNWDFDFRGYKLAGDWQAALGV
                     TVRVHHLTWVSMEGEAKRDYPPSIGYQVPWYKEYSLIEDHFSRLNTVLSRGKPDVKVG
                     VIHPIESYWIHWGPKDKTLLKRAELETNFKNIIEWLLFGLIDFDFISESLLPSLSEVQ
                     ENNRFEVGEMSYEVIIVPCCETLRSTTLERLEAFQKAGGEVIFLGEPPTLVDGVPSDR
                     AKKLADKSSIIPFSRSKLEEKLNKYRDIEIWKEDGTPSDNLFYQMRKEEEKKWLFISH
                     VHKMKNPDIAELEKIKMRIRGEWNPEIYDTLTGEVKECAAEIKDGGTLIKHEFYPHDS
                     LLLKLAPGMPERKERQNDSCSSTGEVNLLDPVSFSLSEPNVLLLDMAEYSFDNGDWQP
                     QEEVLRIDNKFREILNYPLRMNKIAQPWTNKKVEPYEHVLRLKFVVTSRIEIEDLKLA
                     LENPENTKIFVNGEHVESEITGWFVDKSIKKVPIPALPIGDTEIVLEIPFNSQTNVEW
                     CYLLGDFGVEVHGKHAKIVEPVKELTFGSWTEEGLPFYAGNVTYHCPVECDEGELIIE
                     VPQFRAPLLSISLDGEEKGKVAFAPYTLSLGGVEKGKHLIDITAYGNRVNAFGAVHNC
                     DDTYFWFGPNAWRTTGNQWSYEYRLKPVGILVSPRVYIKK"
     gene            2994..3791
                     /gene="larE"
                     /locus_tag="PK016_04050"
     CDS             2994..3791
                     /gene="larE"
                     /locus_tag="PK016_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HXL02276.1"
                     /translation="MKYKKLKENLLALERVIVAFSGGVDSTFLLWVSKETLGENTLGV
                     LVDLPFLPRREREEAEKIARDLKLPLKVIEISGEDEPLIWRNTKERCYFCKKRMMQEL
                     LTLAQKEGYASVVDGTNQDDRGDFRPGFRATQELEIRHPLLEVGFTKYEIREASRKLH
                     LPTWNKPSSSCLATRIPYGVEITLPLLFQIEKGEELLHNLGFSPLRLRHHGEVARLEI
                     SPEQFPLLLEEELRQKVISGLQALGYTFITLDLEGYHSGSMNRLAND"
     gene            complement(3788..4846)
                     /gene="ltaE"
                     /locus_tag="PK016_04055"
     CDS             complement(3788..4846)
                     /gene="ltaE"
                     /locus_tag="PK016_04055"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HXL02277.1"
                     /translation="MKTVTTSQKWIDLRSDTVTLPTEEMLEAMLKAEVGDDVYGDDPT
                     VNLLQEEAAKKMRKEAALFVPSGTFGNQLAIFTHTQSGDEVLIPESNHILEHEVGAPA
                     VISRVGLRPVPDEWGKVDIKDLEKRYREEDLHHPRTGLICMENAHSSGKVVPLSNLQE
                     VYQFAQEREIPVHLDGARIFNAATFLKIEPAEIALYCDSVMFCISKGLCAPVGSLLVG
                     ARSFIEKARKNRKLLGGGMRQVGYLAAAGLIALEKMPRRLSEDHENARYLAKKLKEFE
                     GIKVAEDRLDINMVFFTVDRENFSPSNFLQFLKEKSIKINPPSRGEFRLVTHYGINRE
                     DIDYFIEVSKFYFSTYHS"
     gene            complement(4843..6786)
                     /locus_tag="PK016_04060"
     CDS             complement(4843..6786)
                     /locus_tag="PK016_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXL02278.1"
                     /translation="MKEEPEERVLFSNGKNGGKKTKSLGPVVDLEEHVPPDWWKRIFN
                     SLYLKTDGDVVEDQNITRQEVDYFISILHLSPGDRILDLCCGQGRHSLELARRGFSRV
                     KGLDRSHYLIQKARTQARREGLGVQFKEGDARKLPYPTDSFDAVLILGNSFGYFESTQ
                     DDLKVLTEVFRVLKPGGKIFIDVADGEYLRENFQPHSWEWIGKKQFVLRERSLSKDRQ
                     RLISREIITHIENGVIADQFYAERLYSAKELISLLEKSGFSHVITHGEHAPSSQRNQD
                     LGMMERRIFLSGEAQKEWTRLKRMKGVTLKVAVLLGDPHQPDPLKPQTVFDEDDLYTV
                     NQLKAALGELKNCHFIYLDQHRTLLHDLLQLRGKVDLVFNLCDEGFNNDARQELHVPA
                     LLDILGLPYTGSGPQCLAYCYDKSLVRGIAKELNIPVPCAFLIKPEDFTFKLPFSFPV
                     LLKPNFGDSSFGITQKSVATNLDDFVEAVSEIRNKFGYDKPILVEEFLPGKDLSLGII
                     GNPPESYQILPITEEDYSTLPPGLPPICGYEAKWLPDSPYGKVKSLPSDLPEETEKII
                     VESSLKLFERLECRDYARFDWRLDGEGRPHLLEVNPNPGWCWDGHLAKMANFAGISYH
                     QMLEAILKAAICRLNVITSGDKL"
     gene            complement(6867..7985)
                     /locus_tag="PK016_04065"
     CDS             complement(6867..7985)
                     /locus_tag="PK016_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HXL02279.1"
                     /translation="MASPVYFVNLRAYDERSSVLEKLSRLTRTLLKERLEKGDLVAVK
                     LHFGERGNHGFLKPVFLRYIVEAVKESGGKPFLTDTNTLYLGFRHNAVDHLETAAFHG
                     FGYPAVPAPLIIADGLRGSDYQEVEVNLKHFKTVKVASAIQEADFLLVVTHVKGHIEA
                     GLGGAIKNIGMGGASRVGKQLQHGETFLPDPKEELCIGCQRCIKHCPQNALYLDEKRK
                     ARVKKELCIGCAECVIYCNQEAITTIWSSSASILQERMAEYAYGAVKSKENKVAFINF
                     LTEISPDCDCTPWHDASLVPDIGILGGNDIVAIDKASADLIKKSVGLQNSRLKNAFSS
                     GEDKLLDVHPRTQWEIQIDYGHSIGLGDKIYELKNFPK"
     gene            8330..8800
                     /gene="bcp"
                     /locus_tag="PK016_04070"
     CDS             8330..8800
                     /gene="bcp"
                     /locus_tag="PK016_04070"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003353815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HXL02280.1"
                     /translation="MELKIGMKAPDFTLPDSQGNDVSLSNFIGKNVILYFYPKDNSGG
                     CTAEAIDFRDNYEKIEKTNTVVIGISKDSAKSHLNFAHKYNLPFILLSDKEGKVCELY
                     GVWKEKKLYGRTYMGIERTTFLINQQGFIMKIFPRVKVKGHVEEIVEDLYSLLS"
     gene            9430..11064
                     /locus_tag="PK016_04075"
     CDS             9430..11064
                     /locus_tag="PK016_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL02281.1"
                     /translation="MKSKGMLVLLLTSLLILGTTTAGLTQEKVLVFGSSGDVVRLDPA
                     DVTDGESISRMDNIFEGLVEYEANSTEIKPCLAESWDISEDGKEIVFHLRKGVKFHDG
                     TDFNADAVVFSFERQYNPEHPHHQYGEWAYWGYMFSDIDRVEKIDDYTVKLILSEPNA
                     SIMTSLAMFTVCIVSPANAEKYKEDAFRYPCGTGPFKFVEWVKDDHVTLEANEDYWRG
                     RPKIDKIIFRVIPDPSARLLSLEVGEIHGMEYPNPADFDRIKANSDLVLMSEPGMNIG
                     YMAMNTGYGYVDANKNGVKDPDEPLEKTPGYFEPLTKKEVRQAINMAIDKEAIVRDIF
                     MGTASVAKNGMPPFMLGYNDEIEDYPYDPARAKELLAEAGYPDGFEVTLYVMPVSRPY
                     MFDPPKIGEAIQSYLAAVGVKVNFYQVDWATYLQETEAGMHQMCLLGWTGDNGDPDNF
                     LNVLYGLNACSIGTAGNHAFYTNEENQELLTKALRTYDVEKRVEYYKKAQELIHEDAG
                     WVFLAHSNQNMVFRKNVEGFVLHPASRMFFYPVDIK"
     gene            11231..12235
                     /locus_tag="PK016_04080"
     CDS             11231..12235
                     /locus_tag="PK016_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL02282.1"
                     /translation="MRWYITKRLLMLIPVLLGASILAFSLIHLAPGDPARTIAGEHAS
                     AQTIERIQTKYGLDKPLITQYWIWLKQALRGDFGRSIVSNEYVSKEILSRFPNTLELA
                     LFSMFIAITVGVLAGIVSASRQYSLLDYGFMGVALFGVSMPVFWLGIMLMMIFGVFLR
                     WLPISGRIDIMIPFTRITGLYLLDSIITGNWAAFISSLRHLILPSLALGTIPMATIAR
                     VTRSSMLEVLKQDFIRTERAKGLPERLVIYKHAVRNALIPVVTVIGLNFGLLLSGAIL
                     TETVFSWPGVGRYVVNAVQMRDYPAVQGCVLFFAFIFVVVNLITDIIYVYIDPRIKYQ
                     "
     gene            12269..13141
                     /locus_tag="PK016_04085"
     CDS             12269..13141
                     /locus_tag="PK016_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL02283.1"
                     /translation="MDKESVGSELLRRFKHNKSAVFGLIIMLFMIFCAIAADFIAPND
                     PYPSPPLLANKLKPGFWSDERAPGMPLGSDGLGRCVLSRIIYGARVSLTAGFVAVAIA
                     MILGITFGVLSGYFGGWVDAVIMRIVDIMFAFPALLLAIVIVAVVGQGLEKTMIAIGI
                     VYSPQMARIIRSSVLYIKEMEYIEIQKAIGSSHWRIIFRHILPNSIAPVIVYGTLMLA
                     SAILDCAALGFLGLGAQPPIPEWGAMLADSYSFIVSGAWWAATFPGIAILLSVLGLNL
                     LGDGLRDILDPRLK"
     gene            13152..14156
                     /locus_tag="PK016_04090"
     CDS             13152..14156
                     /locus_tag="PK016_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL02284.1"
                     /translation="MKDNKKKELLKVNNLKTYFYTHEGIIKAVDAVSFSVNQGETLGI
                     VGESGSGKSVTALSIMRLIPDPPGKIVDGEIIFEGRNLCLLSEKEMQKIRGRKISMIF
                     QEPMTSLDPVFTVGHEIMETILLHQKLSKQEAREKAIQMLEVVGIPDAARRMKSYPHE
                     LSGGMRQRIMIAMALSCNPALLIADEPTTALDVTIQAQILRLMNDLKKKFNTSVLIIT
                     HDLGVISEMCDYVAVMYAGHIVEYTDVYTIFDNPLHPYTRGLNRSIPRMDEDRETLET
                     IKGIVPDLLYLPPGCPFHPRCEHALERCFREVPEFTEVEPHHLVKCHMVEREYKSWIN
                     "
     gene            14141..15106
                     /locus_tag="PK016_04095"
     CDS             14141..15106
                     /locus_tag="PK016_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008881547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL02285.1"
                     /translation="MMDKLIEVKNLKKWFPIGKDVAGKKAILKAVDDVSFFINRKEVL
                     GLVGESGCGKTTCGKVLLRIIEPTSGSIFFDNVEITHLNRQEMKNFRRRMMIIYQDPY
                     GSLDPRMRIGKAIAEPLEVHNIGSPKEREERVIQLMEKVGLSADQVNRYPHEFSGGQR
                     QRIGIARALATSPEFIVADESVSALDVSIQAQIINLLKELQQEFGLTLLFVAHDLSVV
                     KHISNRVAVMYLGKIVEMAEKKSLFSEPKHPYTQALLSAIPVPDPRQRKQQEIIMGDV
                     PSPVNPPKGCRFHTRCPKVMDICKVEEPVLRGIKPDYFVACHLCD"
BASE COUNT         4713 a         2811 c         3348 g         4761 t
ORIGIN      
        1 cctgcaatga aaaggccaaa gaagaaggga tgctatgctg gctatacgac gaagacaggt
       61 ggccatcggg ttttgctggt ggatacgtta caaaagataa ccgatacaga gagcactttt
      121 tggtttttac tccttttcca tatgaaaata tggaggaaga aatatcacat ttacttccgg
      181 aaggaaaaac ggttaaaggt aaaaatagga aattgctggc caagtacgaa gtggttttag
      241 aaaatggtta tttagtttat taccagaggt taaaagaaga aacaggaaaa gaaggaaaaa
      301 tctggtatgc ctattcagaa atatcggcag atgacccctg gttcaataat caagcctatg
      361 ttgatacact caatcggaaa gcaattgaaa agttcatcga gataactcac caaaggtatt
      421 atgaaatatt aggtgaagac tttggtaaat ctatcccggc tatctttact gatgagcccc
      481 aatttgtgag gaaagactgt ctgagctttt ctgaagaaga aagagaagtg attcttcctt
      541 tcaccgatga tttagaagaa agttattata aggtgtatca tcaaagttta ttagatcacc
      601 tgccagaatt gatctgggag ttgccagaca ataaagcttc tctcgttaga tatcgctatc
      661 atgaccatgt gacagaaaga tttgttcagg cttttgctga tactatcgga gaatggtgcg
      721 aaaaacataa gattatgtta accggccata tgatggaaga acccactttg gaatcgcaaa
      781 ctgcagctat aggggaagct atgaggccac tcaggtcttt cgggctgcct ggaattgata
      841 tgctgtgtga tcggagagaa ttctccactg ccaaacaagc tcaaagcgta acccatcaat
      901 acggtagaga aggagtttta agcgagatat atggagttac caactgggat tttgacttta
      961 ggggctacaa actggcaggt gactggcaag ctgcgctggg agttactgtt agagttcacc
     1021 acctcacttg ggtatcaatg gagggagaag caaagagaga ttatcccccc agtattggat
     1081 atcaagttcc ctggtataag gagtattctc tgattgaaga tcatttcagc cgtttgaata
     1141 cggtcctgag ccggggaaaa cccgatgtta aggtgggagt tattcatcct atcgaatctt
     1201 actggattca ttgggggccg aaagataaaa ccttacttaa aagagcagaa ttagaaacca
     1261 actttaaaaa tatcatagag tggcttttgt tcgggcttat tgattttgat tttatatccg
     1321 agtcattgct gccttcgtta tcagaagttc aggaaaacaa tcgttttgaa gttggggaaa
     1381 tgtcttatga ggtgataatt gttccctgtt gcgaaactct tcgctccact accttagaga
     1441 gattagaggc tttccagaaa gctggtggtg aggttatatt tttaggcgag cccccgacac
     1501 tggtagatgg tgttccctca gatagggcaa aaaaattagc ggataagtcc tccattattc
     1561 ctttttccag aagtaaattg gaagagaaac tcaataaata tcgtgatata gaaatatgga
     1621 aagaagacgg cactccttcc gataatttat tttatcaaat gagaaaagaa gaagaaaaaa
     1681 aatggttgtt tatttctcat gttcataaaa tgaaaaatcc tgatattgca gagttagaaa
     1741 aaataaaaat gaggataaga ggggaatgga atcctgaaat ctatgacacc ttaacgggtg
     1801 aggtaaaaga gtgcgctgca gaaattaaag acggaggcac attgataaaa cacgaatttt
     1861 atcctcatga tagtttactg ctgaaacttg caccaggaat gcctgagaga aaggaaaggc
     1921 aaaatgatag ctgttcaagt acgggagagg taaatttgct cgatcctgtt tctttttctt
     1981 tatcggagcc caatgttctc ttactggata tggcagagta ctcatttgat aatggagatt
     2041 ggcaaccaca agaagaagtg ctcaggatag ataacaaatt tagagaaatt ctgaattacc
     2101 ctctacggat gaacaaaata gctcagccat ggactaataa aaaagtagag ccatatgaac
     2161 atgtactgag attgaaattc gtagttacct cacgtataga aatagaggat cttaagttag
     2221 ctcttgagaa tccagaaaat actaaaatat ttgttaatgg agaacatgtt gaatctgaaa
     2281 ttactggctg gtttgtggat aaaagcataa aaaaggtccc gattccagct ctccccatcg
     2341 gggatacgga aattgtactg gaaataccgt ttaattccca aaccaatgtg gaatggtgtt
     2401 atctactggg agattttgga gtagaagtcc atggaaaaca tgctaaaatt gtagaaccag
     2461 taaaggagct tacttttgga agttggactg aagaggggtt gcccttttat gctggaaatg
     2521 taacctatca ttgtccggtg gagtgtgacg aaggagaatt aataatcgaa gtgccccaat
     2581 ttagagctcc tctactatca atatctttag atggagaaga aaaggggaag gtggcttttg
     2641 ctccttatac gctaagtcta ggtggggtag agaaggggaa acatttaata gacattaccg
     2701 cctacggaaa tagagtaaat gcttttggtg ctgtgcacaa ctgtgacgat acttattttt
     2761 ggtttggccc gaatgcctgg agaactaccg gcaatcaatg gtcttatgaa tacagactca
     2821 agccagtggg gattttggtt tcgccgcggg tatatatcaa aaaataagct taacgtaatc
     2881 taaagctgaa gtgcgctaac tttctttgct ggttttaagc aaaaaaatac cggatagtat
     2941 taaaacttgg ggaaggtgag acaacggaag aaaatatttg ttcttctgga aatgtgaaat
     3001 ataaaaaatt aaaggaaaac cttttagcgc tggagagggt aatagttgct ttctctgggg
     3061 gagtggattc tacttttctt ctctgggtga gtaaagaaac tttaggagaa aataccttag
     3121 gagtcttagt agatttacct tttttacccc gccgagagag ggaagaagcg gaaaaaattg
     3181 cccgggattt aaaacttcct cttaaggtca tagaaatttc gggagaagat gaacccctca
     3241 tctggcgcaa cactaaggaa cgctgttatt tctgcaaaaa aaggatgatg caggaattat
     3301 tgaccctggc acaaaaagaa ggctacgctt cagtagtaga tggaactaat caagacgata
     3361 ggggagattt ccgccccggt tttcgggcta cccaggaatt agaaatcagg catccacttt
     3421 tagaggtagg atttacaaaa tatgagatcc gggaagcctc tcgaaagctt caccttccca
     3481 cctggaataa accttcatct tcttgtttag ctactcgtat tccttatgga gtggaaataa
     3541 ctctccccct tttgtttcaa attgaaaaag gagaggaact cttgcataac ttaggttttt
     3601 cccccctccg attgaggcat catggagagg tggctcgttt agagatatcg ccagaacagt
     3661 ttccccttct tttagaagaa gagctgagac aaaaagtaat ttctggtctg caagctctgg
     3721 gttatacttt tattactcta gacttagagg gatatcattc tggaagtatg aacagactgg
     3781 caaatgatta actatgatat gtagagaaat aaaatttgct aacttctata aaatagtcaa
     3841 tatcttctct gtttattcca taatgagtaa ccagacgaaa ctctcctcga gaaggagggt
     3901 taatttttat gctcttttct tttagaaact ggagaaaatt tgacggagaa aagttttccc
     3961 tatccacggt aaaaaatacc atattaatat ccagacggtc ttcggccact tttatcccct
     4021 caaattcctt tagtttttta gctaagtagc gggcattttc atgatcttcc gagagacgcc
     4081 ggggcatttt ctccagtgct atcaatcctg cagcggctaa atatcctact tgccgcatac
     4141 ctcctcctaa aagtttacga tttttgcgag ctttctcgat aaaagagcga gcccctacaa
     4201 gtagggatcc tactggggca cagaggcctt tagaaataca gaacatcacc gaatcgcaat
     4261 aaagagcaat ttctgctggt tctattttta gaaaagtagc agcattaaaa atcctcgctc
     4321 catctaagtg tacgggaatt tctctttctt gggcaaattg atagacttcc tggaggttag
     4381 aaagtggaac tactttaccg gatgagtggg cattttccat acagattagc cctgttcgag
     4441 gatgatgaag gtcttcttct cggtagcgct tttccaggtc ttttatatct acttttcccc
     4501 attcatcagg aacagggcgg agtcccaccc gggatatcac cgctggggct cccacttcat
     4561 gctctaaaat gtgattactt tcagggatca atacctcatc cccgctctga gtatgcgtaa
     4621 atatagccag ctgattaccg aaagttcctg aagggacaaa aagagccgcc tcttttctca
     4681 ttttttttgc tgcttcttct tgtagaaggt ttacagtagg gtcgtcaccg taaacatcgt
     4741 ctcctacttc tgcttttagc atagcttcta acatttcttc agtaggaaga gttacagtat
     4801 cgctgcggag gtcaatccat ttttgagaag tggtgaccgt tttcatagtt tatctcctga
     4861 ggtaataaca ttgagacggc agatagccgc ctttaaaata gcttctaaca tttgatgata
     4921 ggaaattccg gcaaagttag ccattttagc taagtgtccg tcccagcacc atccagggtt
     4981 gggattaacc tccaatagat ggggacggcc ttctccatcc agtcgccaat caaaccgagc
     5041 atagtctcga cattctaagc gctcgaacaa tttaagggag ctttccacaa ttatcttttc
     5101 cgtttcttct ggtagatcgc tgggtagaga ctttacttta ccgtagggag aatcaggtaa
     5161 ccacttagct tcatagccac aaatgggggg aagccccgga ggaagggtac tgtaatcttc
     5221 ctcagtgatg ggtaatatct gataagactc gggagggtta ccaataatcc ctaagctcaa
     5281 atctttacca ggaaggaact cttccaccag gatgggttta tcataaccaa acttatttct
     5341 tatttctgat actgcttcta caaaatcgtc taagttagtg gccacacttt tctgggttat
     5401 gccaaaactg gaatcaccaa agttaggttt gaggaggaca ggaaaactaa agggtagctt
     5461 aaaagtgaaa tcttccggtt taatcaagaa agcacaagga acaggaatat tcagctcttt
     5521 ggctattccc cgaactaaag atttatcata gcaataagct aaacattggg ggcctgaacc
     5581 ggtgtaaggc aaccccagta tatctaaaag agcaggaaca tgaagttctt gtcgggcatc
     5641 attgttaaat ccctcatcgc aaaggttgaa aaccaggtct accttccctc gcagctgaag
     5701 taaatcgtgc aataaagtac ggtgctgatc taaatagata aagtggcagt tttttaattc
     5761 tcctaaagct gcttttaatt ggttgacagt ataaaggtcg tcctcatcaa aaacagtctg
     5821 aggcttaaga gggtcaggtt gatgagggtc gcccagtaat actgctacct ttaaagtgac
     5881 acctttcatc cttttaagac gtgtccattc tttttgagct tcgccactta agaaaattcg
     5941 tctttccatc attcccaggt cctgattccg ctgagaagaa ggagcatgtt ctccgtgagt
     6001 gataacgtga ctaaacccgg acttttctag aagagatatt aattctttag cagaatacag
     6061 tctttcggca tagaattggt cagctattac tccattttca atgtgggtaa ttatttcccg
     6121 ggaaattaaa cgctgccggt cttttgataa agaccgttct cgcaatacaa attgtttttt
     6181 accaatccat tcccaggagt gaggttgaaa attttctctc aggtattcac catcagctac
     6241 atcgataaag attttgcctc caggtttaag cactctaaaa acctctgtca gcactttcaa
     6301 atcatcttga gtgctctcaa agtatccaaa actatttcct aaaatcaata ccgcgtcaaa
     6361 actatcagta gggtagggta gcttacgagc atctccttct ttaaattgga cacctaatcc
     6421 ttccctccgg gcttgggtcc tggccttctg gattaagtag tgagaacggt ctaacccttt
     6481 tacccggcta aatccccgtc gagctaattc taaagaatgc ctgccctgtc cacagcaaag
     6541 gtctaaaatt cgatctccag gagagaggtg aagaatactg atgaaataat ctacttcctg
     6601 tctcgtaata ttttgatctt ctactacatc tccatcagtt ttcaaataaa gagaattaaa
     6661 aattcttttc caccaatctg gtggaacgtg ttcttctaaa tccacaactg gtcctaaaga
     6721 cttggtcttt ttgcccccat ttttaccatt agaaaataaa actcgttctt cgggttcctc
     6781 tttcattttc ctccaccttg ctcaggagat tttttattat agatgattat agaaaaatgg
     6841 aattaaaaaa caatatggga ataaaattat ttggggaaat tttttaactc ataaatttta
     6901 tctcctaaac caatggagtg tccatagtca atctgtatct cccactgagt ccggggatga
     6961 acatctaaaa gtttatcctc cccagaagag aaagcgttct tcaaccttga attttgtaac
     7021 cccactgatt ttttgattaa gtcagcagaa gctttatcaa tagcgacaat atcatttcct
     7081 cctaaaattc caatatcagg taccaagctg gcgtcatgcc agggagtgca atcgcaatca
     7141 ggacttattt cggtgagaaa attaatgaaa gctaccttgt tttctttact ttttactgct
     7201 ccataagcat attctgccat ccgctcttga agaatagaag cagaagaaga ccagatagta
     7261 gttatggctt cttgattaca gtaaataaca cactcagcac aaccaataca taactctttt
     7321 ttcacccgag ctttccgttt ttcatcaaga taaagagcat tctgagggca gtgcttgata
     7381 catcgctgac aaccaataca taattcttct ttagggtcag ggagaaaggt ttctccgtgt
     7441 tggagttgtt taccaactcg agaggcacca cccatgccta tattttttat agctcctccc
     7501 aagcccgctt ctatatgtcc tttaacgtgg gttaccacta aaagaaaatc ggcttcctgg
     7561 atggcagaag ctacttttac tgttttaaaa tgctttaaat taacttctac ctcttgataa
     7621 tcacttcctc gcagaccatc agcgatgata agaggggcag gaacagctgg gtaaccaaaa
     7681 ccgtgaaaag cggcagtttc taaatggtct acagcattgt ggcgaaagcc taaatacaaa
     7741 gtattggtat cagttaaaaa gggtttacct cctgattctt ttaccgcctc tacaatataa
     7801 cgcagaaaaa ctggttttaa gaaaccatgg ttacctcgct ctccaaaatg gagttttact
     7861 gccaccaaat ctcccttttc caatctttct tttagaagag ttctggttaa cctgcttaat
     7921 ttttctaaaa cagaacttct ttcgtcataa gctcgtagat taacaaaata taccggtgaa
     7981 gccatatcaa gcctccttta gttgtgattt ctgacaaatg tattctccag tattttacca
     8041 gtttttgaaa gtaatgatca taagaggtag cagaatagtc tgagcataat gctggatatc
     8101 gaccggagag ctttttctat tgtagttgac cgcaaaagtt atgtgatact agagaaaacg
     8161 ttttatccct cagtgttttt tttgaggtag cagaggaggt cattcaatgg cagatgttga
     8221 attattgcca aacattttag gtaaaccccc tgcacctgta ttctcgtgta tgatatgatt
     8281 aaattaaaac aattttgagg aagttgatct aaaataatag gagaaaaaaa tggaattaaa
     8341 aatagggatg aaagctcctg attttacttt gcctgatagc caggggaatg acgtttcctt
     8401 atctaatttt atcggtaaga atgttatttt atatttttat cccaaagata acagtggtgg
     8461 atgtacggca gaagcaattg atttcaggga taattacgag aaaatcgaaa aaactaatac
     8521 tgttgttatc ggcataagca aagatagcgc caaatcccac ctcaactttg ctcacaaata
     8581 taatttgcct tttatattac tcagcgataa ggaaggtaag gtttgtgaat tgtatggtgt
     8641 ctggaaagaa aagaaattat atggacgcac atatatgggt attgagagaa ccactttttt
     8701 gattaatcag cagggattta taatgaaaat atttcccaga gttaaagtaa aaggtcatgt
     8761 ggaagaaatt gtggaggacc tatattcatt gctttcttag cacttgtcgt aacaaaacct
     8821 ttctcataag tttgttctga attcgaataa gtaaaatata cacccatccg tctcagaaat
     8881 tgctgatcac tggttagact ggtataataa aaattgtttt attttataac agctattaga
     8941 ttgattaata aaattgatgg taaagtttgg gggtcttatc caagagagtt gctcctagta
     9001 aagggggtct ttgatgatta agaataaatg ttcgatatca tattggagca aaattaatag
     9061 ctgttttcag ataaccttcc aaaatctttt cagttctggt aatttttgga attggggttt
     9121 atttttaaaa agaaggtatt ttttactctt caagagtaaa cttttactcc actttattac
     9181 ctctttttct ttcttggata aagttcttta tttcagaaaa gtattctgta cctatctttg
     9241 ttccactttt ctatcaacta tttgggagag gggcttattt ttttaaacac tgataaagaa
     9301 ggaggttctc tttaaaaaaa taagaacgag gaaaatcact ggtattttag aaaaaaggag
     9361 gtgatgttat tttaactttg cgagattaat aattatagct ttgttttaat aagaaaggag
     9421 aaaaaaagaa tgaaaagtaa agggatgtta gttttattat tgacctctct tctgattctg
     9481 ggtacaacaa cagcaggtct aacacaagag aaggtcttgg tttttggaag tagtggagat
     9541 gtggtccgtc tggatccggc agacgtaacc gacggtgaat ctatctcaag aatggataat
     9601 attttcgaag gactggtgga atatgaagct aactcaacgg agatcaaacc ctgcttagca
     9661 gaatcatggg atatatctga agatggtaaa gaaattgtct ttcatttaag aaaaggggtt
     9721 aaattccatg acggtaccga ttttaacgct gatgcagttg ttttctcctt tgaacgccag
     9781 tataatccag aacatcctca tcatcaatac ggggagtggg cctactgggg ttatatgttc
     9841 agtgatattg atagggtgga gaaaattgat gattacaccg taaaactcat cttaagtgag
     9901 cccaatgcct ctataatgac cagtctggcc atgtttactg tttgtatcgt cagccccgct
     9961 aatgcagaaa agtataaaga agatgccttt cggtatccct gtggaaccgg tcctttcaaa
    10021 tttgtagaat gggtgaaaga cgaccatgtt accctggaag ctaatgagga ttactggagg
    10081 ggaagaccga aaattgataa gataattttc agggttattc cagatccttc tgcccgttta
    10141 ctgtctttag aagtgggaga aattcatggt atggaatatc ccaatcctgc tgactttgac
    10201 cgtattaaag ctaactccga tcttgttttg atgtctgagc cgggcatgaa tatcggctac
    10261 atggctatga acactggtta tggttatgtg gatgctaata aaaatggagt gaaagaccct
    10321 gacgaaccac tggagaagac acctggttac tttgagcctc tcaccaaaaa ggaagtcaga
    10381 caggccatta atatggctat agataaagaa gccattgtta gggatatctt tatgggtacg
    10441 gcttcggtgg ccaaaaacgg aatgcctccc tttatgctgg gatacaacga tgaaatagaa
    10501 gattatcctt acgatccagc aagagccaag gaactattag ctgaggccgg ttatcctgat
    10561 ggttttgaag ttaccctata tgtcatgcct gtctccagac cttatatgtt tgatccaccc
    10621 aaaattgggg aagctataca gagctatctg gcagcagtgg gagttaaagt taatttctat
    10681 caggtagact gggcaaccta cttacaggag accgaagctg gtatgcatca aatgtgtctt
    10741 ttgggatgga ctggtgataa tggcgatcct gataactttt tgaatgtcct ctatgggctt
    10801 aatgcttgct ccattggaac agctggaaat cacgcctttt ataccaacga agaaaaccag
    10861 gaacttctga ctaaagcgct aagaacttat gacgtagaaa aaagagtgga atactacaag
    10921 aaagcccagg agttaataca tgaagacgcc ggatgggttt ttctggctca ctctaaccag
    10981 aatatggtat tccggaagaa tgtagaaggc tttgtgttac atcccgcttc cagaatgttt
    11041 ttctatccgg ttgatataaa gtaatagctg ataaaaggga tgaactatat tggttcatcc
    11101 cttttattta tgagtgtgcc cggatagctt ggtggtgaaa gtctgctctc tatcctaatt
    11161 tgtgtatgta gttataattc atatgataga ataaactatt atttgaaatg gagaaaataa
    11221 atttggccag atgagatggt atattaccaa aagattgttg atgttaattc ctgttctatt
    11281 aggagcttct attttggcat tctctttgat acatttagca ccaggggatc cagcaagaac
    11341 cattgctgga gaacatgcct ctgctcaaac cattgaaaga attcaaacaa aatatggtct
    11401 ggataaaccc ctaataaccc aatattggat ctggcttaaa caggccttac ggggcgattt
    11461 tggtcgttct attgtttcca atgaatatgt gagcaaagag attttgagcc gttttcctaa
    11521 tactcttgag ttagcactgt tttccatgtt tattgccata accgttgggg tattagcggg
    11581 aattgtttct gcttcccggc agtattcttt attagattat ggctttatgg gtgttgcttt
    11641 attcggggtg tccatgccgg ttttctggct gggtattatg ttgatgatga ttttcggagt
    11701 gtttttgcgc tggctaccaa ttagcggtcg gatagatata atgattcctt ttaccaggat
    11761 aaccggctta tatcttttag atagtattat taccggtaat tgggcagcgt tcataagttc
    11821 cctgcgtcat cttattctcc cctctcttgc tttaggcacc attccgatgg ctaccattgc
    11881 tcgagtaact cgttccagta tgctggaagt actaaaacag gattttatta gaaccgaaag
    11941 agcaaaaggg cttcctgaaa gattggtgat atataaacat gcggtaagaa atgctttaat
    12001 tccagtggtt accgttattg gtttgaactt tggtttgctt ttgtcgggcg ctattttaac
    12061 tgaaacagtt ttttcctggc ctggtgtagg tcgttatgtg gtaaacgcag tccagatgag
    12121 agattatcct gctgtgcagg ggtgcgtatt gttttttgcc tttatttttg tagtggtaaa
    12181 tctaataact gacattattt atgtttatat tgacccgaga ataaaatatc aatagctggt
    12241 ctcgtatata aaaaaataag gagttacaat ggataaagaa agcgtgggaa gtgaattatt
    12301 acgccgtttt aaacacaata aatctgctgt ttttggttta ataattatgc tttttatgat
    12361 cttttgcgct attgctgctg attttatcgc ccctaatgat ccttatccat cccctcctct
    12421 tttggctaat aaattaaaac caggattctg gagtgatgag agagctccgg gtatgcctct
    12481 tggttctgat gggctgggga gatgtgtctt aagtagaatt atttatggtg ccagagtcag
    12541 tttaacggct ggttttgtgg cagttgctat agctatgatt ctgggaatta cctttggggt
    12601 tttatctggt tattttggtg gctgggttga tgcggttatt atgaggatag tggatattat
    12661 gtttgctttt ccagctttat tgttagccat tgtcattgtc gcagtagttg gtcaaggact
    12721 ggaaaaaacc atgatagcaa taggtatcgt ttattctccc cagatggcta gaattattcg
    12781 tagttcagtt ttatatatca aggaaatgga atacattgaa atacagaagg caattggttc
    12841 aagtcactgg cggataattt ttcgccatat tcttcctaac agcattgctc cagttattgt
    12901 ttatgggact ttaatgttag ctagtgcaat tttagattgc gctgcactgg gctttttggg
    12961 tttaggggct caacccccta ttcccgaatg gggagctatg ttagctgaca gctattcttt
    13021 cattgtgagt ggagcctggt gggcagcaac ctttccaggg atagctattc ttttatccgt
    13081 cttggggctc aatttattgg gtgatggact gagagatatt ttggatcccc gattaaaata
    13141 atataataga tatgaaagac aataaaaaga aagaattact taaggtaaat aatttaaaaa
    13201 cctactttta tacccatgaa ggaattatta aagctgtaga tgcagtatca ttttcggtta
    13261 atcagggaga aactctgggt attgtgggtg agtccggctc gggtaaaagc gtaaccgctc
    13321 tctcaattat gcgcttaatc cctgatcctc ctggaaaaat tgtcgatgga gaaataatat
    13381 ttgaggggag aaatttgtgt cttctcagcg agaaggaaat gcaaaagatt cggggtagaa
    13441 agatatcaat gatttttcaa gaacccatga cttctctgga tccggttttt acagttggac
    13501 atgaaattat ggaaactatc ttattacatc aaaagctatc caaacaagag gccagggaaa
    13561 aggccattca aatgttagag gtcgttggga taccagatgc agcgagaaga atgaagagct
    13621 atcctcatga gttatccggt ggtatgcgac agcgaataat gattgctatg gctttatcct
    13681 gtaatccggc attattaatt gctgatgagc caactactgc actagacgtt actatccagg
    13741 ctcaaatttt acgtttgatg aatgatctaa agaaaaaatt taatacttct gttttgatca
    13801 ttacccatga tttaggggta atatctgaga tgtgtgatta tgtggcggtg atgtatgccg
    13861 gacatattgt ggaatatact gatgtttata ccattttcga caatccgctg catccttata
    13921 ctagaggact gaatagatcc attcccagaa tggatgaaga tagagaaact ttagaaacta
    13981 ttaagggtat agtgccagat ctactttatt taccacctgg ttgtcctttc catccccggt
    14041 gtgaacatgc tttggaacgt tgttttcggg aggttccgga atttactgaa gttgagcccc
    14101 atcacctggt taagtgccat atggtggaaa gagagtacaa atcatggata aattgattga
    14161 agtaaaaaat ttaaaaaaat ggtttcctat aggaaaagat gtcgctggaa agaaagcaat
    14221 tttaaaagcg gtagatgatg taagtttctt tattaatcgt aaagaagttt taggtttagt
    14281 tggtgaatct ggatgtggaa aaactacttg tggtaaggtg cttttaagaa ttattgaacc
    14341 aacttctggt tccatcttct ttgataatgt agaaattact catctcaatc ggcaggagat
    14401 gaagaatttc aggcgtagaa tgatgattat ttatcaggat ccctatggtt cgcttgatcc
    14461 cagaatgaga ataggtaaag caattgctga gcccttggaa gttcataata ttggctcacc
    14521 taaagaaagg gaagaaagag ttatccaact aatggaaaag gttggtttgt ctgccgatca
    14581 ggtcaatcgt tatccacatg aatttagtgg gggacaacgc cagagaatcg ggattgccag
    14641 ggctctggct actagtcctg agtttatcgt agcagatgaa tctgtctctg cattggatgt
    14701 ttccatacaa gctcaaatta ttaatctatt aaaggaatta cagcaagaat ttggcttaac
    14761 tttgctcttt gttgctcatg acctgagtgt ggtaaaacat attagcaatc gggtagcagt
    14821 aatgtattta ggtaaaattg tagaaatggc cgaaaagaaa tctctattta gtgagccgaa
    14881 acatccttac actcaggcat tgctttctgc cattccggtt cccgacccaa ggcagagaaa
    14941 gcaacaggaa attataatgg gggatgttcc tagccccgtt aatccaccta aagggtgtcg
    15001 ttttcatact cgttgcccga aagttatgga tatctgtaag gtggaagaac cagttttaag
    15061 gggaattaaa cccgattatt tcgtcgcctg tcatctttgt gattaaataa ggttgttgtg
    15121 tagttttcag gaatactgat aacgctaaat ttttcagggt tcttctccca aatagttgat
    15181 ggaaaagtga aacaaagata ggcatagcct tcttttcctg gataaagtat tctatccagg
    15241 aaaagaagta atcaaaggaa gttagtattt gcttttgaag aataaagagc accttctaaa
    15301 gagaaaccaa actctagaaa ttactaaaac cgcagaggtt ttggaaaccc atctaaaatc
    15361 aattattgat tttatgtgtg tggtaggcaa gcaaacatag aataagaaag aagttctaaa
    15421 gattactttg tcttttccaa acctaatcct tcgtgttcta tgtaccactg gttggtagcg
    15481 cttcaaagta agtaccaaaa gaaagagaat ggaaatagag gaattcttct acggtgtttt
    15541 tacctccaaa ccctgactca atttatacat aattgctcac ccctttactc taattccgtt
    15601 tataaatgta gtttttactt gaaagtggtc atc
//
LOCUS       DAOSVE010000032        15553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_2328_length_15553_cov_70.648084, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000032 DAOSVE010000000
VERSION     DAOSVE010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 15553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15553
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2328_length_15553_cov_70.648084"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            378..911
                     /locus_tag="PK016_04100"
     CDS             378..911
                     /locus_tag="PK016_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HXL02286.1"
                     /translation="MKSREITKISLLVATGVVLPSLFHLSGIPGVVFLPMHLPALLGG
                     FFLSLGSAAFLLGFILPLLNFFLTGMPPFPNFLVMMAELGGYGILIFFLYRKRRWGIF
                     PSLFASLLGGRLISILGNWVLIVLVLGKKFSLLPVLNTLFVISLPGIAIQIVLIPLIV
                     KGVENFAQRRTANTGNF"
     gene            874..1470
                     /locus_tag="PK016_04105"
     CDS             874..1470
                     /locus_tag="PK016_04105"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXL02287.1"
                     /translation="MLSEEQQIRETFNRLAWEWDERVFSSPDKIRYLLSFLTISPSST
                     ILDVGSGTGVLIPFLRERMGSSGLIVELDIAEEMLKVAREKFGEEGIGYHRKSVEKYK
                     SSRRYDLVIVYSAFPHFWDRDLALQNLISHLKEGGELAIAHSESRETINQRHRGDGAP
                     QAFLLPPGGELAQELKKRGLEITRIIDNQELYLVVGRK"
     gene            complement(1482..1883)
                     /locus_tag="PK016_04110"
     CDS             complement(1482..1883)
                     /locus_tag="PK016_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HXL02288.1"
                     /translation="MNQVEKILCPVCQVSFILREEKEVGKRIICPVCGAVLVLEKDNN
                     TWVARRPKDLIPEEEIRARMENFARLRGYKFNELKEPLVEGLLKKYERYGDFYCPCKI
                     DNIPENVCPCLETRQGSVERNGRCHCGLFWK"
     gene            complement(1880..2128)
                     /locus_tag="PK016_04115"
     CDS             complement(1880..2128)
                     /locus_tag="PK016_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HXL02289.1"
                     /translation="MKKVMLYALSTCPFCQMTKKYFEKHNIPYDFVDVDLLEPEEKEK
                     TVAKVQKISGRRAFPVIVIGDKVIVGYDELSISEALKE"
     gene            2270..3076
                     /locus_tag="PK016_04120"
     CDS             2270..3076
                     /locus_tag="PK016_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HXL02290.1"
                     /translation="MFDIRAVSTNTYHGCSLEEALSGIAKAGFSCVELACVEGWTNHF
                     SIINYREEDLKSLRSSLSRLGLRVVSISAHSDLSTPEGVSYLEKALDLARILGAQVVN
                     TGTIEEEKKKGILISNLQVLAPEAEKRGVKIGLEIHGNFLKDGLRARELMEELNHPAV
                     GINYDTGNAIFYGGVCPEEDVKNCLPWIVHFHLKDKRGGYKVWDFPPLGEGDIDFGKI
                     FALAREEKIEVPWSVEIEFDGKFQHSREFVDQAVKKSFDYLENLLKKGKE"
     gene            3081..3317
                     /locus_tag="PK016_04125"
     CDS             3081..3317
                     /locus_tag="PK016_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02291.1"
                     /translation="MIYSGEKEQTPLMIYRNGETKKEIEVRKEDPYYLELKYFLDCVE
                     NNKLVEKGSPEQAILALVARDSAEKGEMVKFSRQ"
     gene            3317..4591
                     /locus_tag="PK016_04130"
     CDS             3317..4591
                     /locus_tag="PK016_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXL02292.1"
                     /translation="MRKILLVEKSALWREKIGNWLREEGYYLIAVRDRDVAINCLEKE
                     EIDIILVEEKVLREDSFSLLSFIRQSFPDMVIIALGESNDSFPLQELFSKGVYDYIST
                     SFAPSRILSVIKRAEEKISLLEENKDLKKKVAGRPSFAGITGVSEKMQRVFSLILQVS
                     QVKRPILLVGEQGTGKELVARAIHQYAFSEEKPFFKVLCATLPLEILPREKPEEGTIY
                     FEDVHLLPYKQQAEMLEFLEEYRFGKKSSDLRIIASSEVPLEEKLAEGSFRDDLYYFL
                     NAVKIEIPPLRERKEDIPFLVDRFLQEIEEETGKEEIRVDKEALRFLIEYDWPGNVIE
                     LRNTLEGMVILSSSDLLTSEDLPDYIKKRQREELLGVKVGMSLEEVEKILIQETLKFT
                     RFNKTRAAHILGISLRTLYRKMEQYNLSLRRK"
     gene            4769..5059
                     /gene="groES"
                     /locus_tag="PK016_04135"
     CDS             4769..5059
                     /gene="groES"
                     /locus_tag="PK016_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HXL02293.1"
                     /translation="MKIKPLGDRILVQRVEEEEVKKGGIIIPDTAKEKPQQGKVIAVG
                     TGKVSEDGKRMPLEVKEGDKILFGKYAGTEVKIGDEEYLIMREDDVLGIIEG"
     gene            5076..6695
                     /gene="groL"
                     /locus_tag="PK016_04140"
     CDS             5076..6695
                     /gene="groL"
                     /locus_tag="PK016_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907993.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HXL02294.1"
                     /translation="MMAKQILFDEEARQKVLGGVKTLAEAVKVTLGPRGRNVIIEKKF
                     GSPLITKDGVTVAKEIELEDPNENVGAQLVKEVASKTSDIAGDGTTTATVLAEYIYRD
                     GLRAVIAGSNPMLLKRGIDKAVDAVTKKLKSISKEVSGKKEVAQVATIASNNDESIGN
                     IIADAMEKVGKDGVITVEEAKGLETTLEVVEGLQFDRGYLSPYFVTDPDRMEAVLEDT
                     YILIYEQKISAVKDLLPILEKVVQRGRPLLIIAEDVEGEALATLVVNKLKGVINCVAV
                     KAPGFGDRRKEMLQDIAVVTGGTFISSDLGIKLENITLDDLGQARKVVVDKENTTIVE
                     GAGKKEDIMARMNQIKKQIEETTSDYDREKLQERLAKIAGGVAVIRVGAATEAEMKEK
                     KARVEDALNATRAAVEEGIVPGGGVALIRCESAINELSLDGDEKIGALVVKKSLSEPA
                     KMIAQNAGEEGSVIVEKIKASDNLNFGYNALTGKFVDMLEDGVIDPAKVVRVALQNAS
                     SVAGIMLTTESVVTEIPEKEKVPVAPPMPEY"
     gene            6781..7053
                     /locus_tag="PK016_04145"
     CDS             6781..7053
                     /locus_tag="PK016_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013473915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HXL02295.1"
                     /translation="MPIYEYQCTQCGKTFEELQSFSDKPVTQCPFCKGRVKKLIGRNV
                     GLVFKGSGFYCTDYRSSGSSSSTNSSSCSTCSSSNCSTCSGGSSSQ"
     gene            7059..7691
                     /locus_tag="PK016_04150"
     CDS             7059..7691
                     /locus_tag="PK016_04150"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HXL02296.1"
                     /translation="MDLWGYLLIAGLLVFFASLRGVKRRGSSVSVSEFWQEKEKEFGE
                     KKILSGFSRYLGGHPRFEAISDGLLFLMSRSLWFENFEKGPSIFGIAPPFKKVIFRIL
                     LSQIEDVESLPEKELLKRGFDSRQLWKYHRLSRRPFYLAVKYRDEWEKEKTLYFDSMI
                     DADIWREELEKAKSNYVPEEGIKESGVCPQCGKKIDPDFKLCPYCGCGLS"
     gene            7705..8592
                     /locus_tag="PK016_04155"
     CDS             7705..8592
                     /locus_tag="PK016_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HXL02297.1"
                     /translation="MSFQVGHYQDFSKATGCTVFLFEEPNRAVAAVRGGAPGGREIST
                     LFPGNLVEGVDAVFFSGGSALGLSCGEGVVDFLRERKKGFKTPGGIVPIVAGAVIYDL
                     EVGEPYSPDREWGYKAALSIEEVIKEGTIGAGTGATVGKLMGMGKAVKGGLGRGKAKA
                     GGEEISAFVVTNSLGNIYHPQTGELVAGVKIERKGAAISPFNTTLTLVVFDCFLSREE
                     LFSLMGVVHSALASCIRPFATLYDGDTIFLVSTGKKRVNDYLALVSGIYESVGEAVLN
                     SVTKATGLAGILAKNDIIV"
     gene            8683..9186
                     /locus_tag="PK016_04160"
     CDS             8683..9186
                     /locus_tag="PK016_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HXL02298.1"
                     /translation="MERRTKKFVLAGVLGAVSVILSLTPLGLIPVPNLASYATTMHIP
                     AIIGGIIGGPEVGALVGLILAFSTLHLFAGNILACFLPRLLIGVVAYYLWQWSGRQDW
                     GIVLGSLGGTFTNTIGVLSLMVVTGYFTWAQVLPVFVLNGVLELIISGILVLPLVRIL
                     KRVVKFE"
     gene            9188..9949
                     /locus_tag="PK016_04165"
     CDS             9188..9949
                     /locus_tag="PK016_04165"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HXL02299.1"
                     /translation="MLVIDVGNTHTTWGVYEDKILLSHWRVSTSSHRTSDEWSFVFRN
                     LLEEEGIKRATLYRVVVSCVVPPVLGALEYLFREKWRLPYLSVGPGIKTGLSLRVEAK
                     EVGADRVVNALAASSLYGGDLIIVDFGTATTFCAVTKNKEYLGGVIAPGISISAEALY
                     EKTAKLPRVDIEIPSHIVGKNTVEAMHSGVFFGHIGLSREIVKRMKEEFSLEAKVIGT
                     GGWSIFLHRYCDFIDIFDPVLTLEGLRIIYELNQE"
     gene            9930..10298
                     /locus_tag="PK016_04170"
     CDS             9930..10298
                     /locus_tag="PK016_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HXL02300.1"
                     /translation="MSSTKNEEQELAIVGGQSKISGSFEGGGFLVVEGEFAGSIQCRE
                     LVITRKGKIEAWVEVENMEVWGEVNGFSRAKKAICRKTSRIGGCIMGSGIALEPGAII
                     EGALTLEKVEEIEGEGKESS"
     gene            10276..11181
                     /locus_tag="PK016_04175"
     CDS             10276..11181
                     /locus_tag="PK016_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007956159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate 5-dehydrogenase"
                     /protein_id="HXL02301.1"
                     /translation="MKEKRVVSASLGSSKRNHRAEIELLGCKVFIERIGVDGDKNKFV
                     ELLQSLDGKVDAIGLGGADLYLRAGKYRYEVEDVARLVSVLKETPIVDGGELKETWER
                     EVPRCLREEEEIELKGKTALIMSGMDRYGLAEGLYQEGCHLLIGDLPFAVGVPGALRR
                     LGTLRFLSVLMMPVLRRLPIDVLYPTGKRQEERKPRFSGYFEKADIIGGDFLYIWRFM
                     PDRLPGKMIITNTVTSQNISEMKERGIKILVTTTPEINNRSFGANVLQAIFVAVLERK
                     PEDIKPEEYLDLMKELHIKPRVIKL"
     gene            11241..11744
                     /locus_tag="PK016_04180"
     CDS             11241..11744
                     /locus_tag="PK016_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HXL02302.1"
                     /translation="MTIRKAQLEDLQQITEIYNQAVLKTISTFHLYPRSWEEQVNWFK
                     SHGDKYPLLVAEESGSVIAWACLSPYSDREGYQYTVTDSIYVREEFRRQHIGYELLNI
                     LIGEAQKLGYHSVIAFIARENLASIGLHEKVGFVYRGELKEAGFKFGRWMDVCFYQRM
                     LERGERR"
     gene            11741..12274
                     /locus_tag="PK016_04185"
     CDS             11741..12274
                     /locus_tag="PK016_04185"
                     /inference="COORDINATES: protein motif:HMM:NF016024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rad52/Rad22 family DNA repair protein"
                     /protein_id="HXL02303.1"
                     /translation="MKNREILEKKFPKKEVKTRPGREGREVYYLETASVIQRLNEAWE
                     GDWSFEVKDKGIDKEAGYVWVLGRLTCGEVIKEQFGSKIISLSSDGTMVDLGDDLKAA
                     ASDALKKCATLLGVGLYLYQEEEEETFPRPASQRQKDFLRNLLKERGKVVDEEMLETI
                     SSVEASRLIEELKEKRP"
     gene            12296..12838
                     /gene="amaP"
                     /locus_tag="PK016_04190"
     CDS             12296..12838
                     /gene="amaP"
                     /locus_tag="PK016_04190"
                     /inference="COORDINATES: protein motif:HMM:NF033218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HXL02304.1"
                     /translation="MNFWGKFFTFIVGIILIGKALGIVAMTFKLVPLLTLQEWVVATY
                     LFFSQDIFSQILGVILAVILVFVAFLVFRLLISKPEKTLVYHTEQGDIKVSFSSLQSL
                     AQEALRSFDEILQSRAEIEKVGNEARLILHLAVKPEASIPELSPAVQNKVKEKIERQT
                     GVTVKEVKLTIDLKPEEEAV"
     gene            complement(12812..13426)
                     /locus_tag="PK016_04195"
     CDS             complement(12812..13426)
                     /locus_tag="PK016_04195"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HXL02305.1"
                     /translation="MANNAISLEKWGAKLFPHRGIIWGALGLIIFLFAHPHHPLFSWG
                     IALTCLGEGLRIWGVGYIKNYRGPMQEVERLTTAGPYAYLRNPLYLANGIIGTGIAII
                     SGLWWSIILFWFAFYLLYFPIIQAEEKFLENNFGETYKAYIRRVPRFFPRFSPYPDRN
                     GTFSWRVIGKEETTTLFTLGAAVLFFYLRGFGFLKILDRLFLGF"
     gene            complement(13402..14394)
                     /locus_tag="PK016_04200"
     CDS             complement(13402..14394)
                     /locus_tag="PK016_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase family protein"
                     /protein_id="HXL02306.1"
                     /translation="MEKVGKIPPSVLERLVFPFQGKKRKEVIWGAQLGRDCGIVQLGK
                     ELISLTCDPITGSSFHLGLLATQVVVNDLVCSGAEPVALLVTLIFPPGISEKEIEIIM
                     RELDEVGKELGVSIIGGHTEISEAVNRSIIHCTGLGWVKDNHLPDVSKIEAGDYIVMT
                     KGAGIEGTAILFSEKGKEWQHLLDEETINKGKEFWKMMSVFQEGKIALPFHPHALHDA
                     TEGGVLGAIWEACTSKGLGFEIEEEKILVYPQTRVLAQLLNLDPLKLISSGTLIIFTP
                     APLPLLQALREVVPAEVIGRVKKDPSRILIHQNGEKEEIKECPQDELWRIMQSA"
     gene            complement(14378..14806)
                     /locus_tag="PK016_04205"
     CDS             complement(14378..14806)
                     /locus_tag="PK016_04205"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HXL02307.1"
                     /translation="MRELESLDKWSKSLSFKSRHDFLNRFLRECPVEVFLPPLVTLEG
                     NIETRGLVRVEGILKGKVKCPIIVIAENARATVEVEAQCVYVEGFLRGIVNSSFFYLS
                     SSGKVEGEIKTEILFVDQGAWMKGKLLIEKGRNHGDGKSG"
     gene            15402..15479
                     /locus_tag="PK016_04210"
     tRNA            15402..15479
                     /locus_tag="PK016_04210"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15437..15439,aa:Pro,seq:tgg)
BASE COUNT         4528 a         2622 c         3669 g         4734 t
ORIGIN      
        1 agaaggctaa ggtttgttag aaccgatggc ttttttattc cgcttaaact ttgatagccc
       61 ttttaaaaat aacgtctcaa gttattgtgg tgcgctaaaa attatgtttc cttccttatc
      121 tactatctac tattgccaga ctctcggttt tgagagttcc agtatttatt ccccgaaaat
      181 aaattattcc agttgaagat gcccccggat catgatttat tacccgccta ctgtaaagat
      241 ggtctttcga taccgaaaca ttggaggaaa agcaaaggcg tgacacctct cgctactcaa
      301 gccagagaat tttagcttcc ttgacaggtt atgattcgat gttacaatct tccaatggag
      361 cgattgttga ataataaatg aagtctcgag aaataaccaa aatttctcta ttagttgcta
      421 caggagtcgt tttaccttct ctttttcatc tttctggtat tcctggggtg gttttcctac
      481 ctatgcattt acccgctctg ttaggaggtt tttttctttc cttaggaagc gctgcttttt
      541 tactgggttt tatacttccc ctgctcaatt tttttcttac cggtatgcct ccttttccta
      601 actttttagt tatgatggca gaactggggg gatatggaat attaatcttc tttttatata
      661 gaaaaaggag atggggtatt tttccttctc tctttgcttc tcttctgggg ggaagattaa
      721 tttccattct gggtaattgg gttttgattg tcttagttct gggtaaaaaa ttctccctcc
      781 tcccggtttt aaatacctta tttgtgattt cccttcccgg gatagctatt cagatagttt
      841 taattcccct gatagtgaaa ggagtggaaa attttgctca gcgaagaaca gcaaatacgg
      901 gaaactttta atcgtttagc ttgggagtgg gatgagcgtg ttttctcttc tccggataaa
      961 attcgttatc tcttgtcttt tctcaccatt tctccttctt ctactattct cgatgtaggc
     1021 tcaggtacag gggtattaat tccttttttg cgagaaagaa tgggttcctc tggcttaatt
     1081 gtagagttag atatagctga agaaatgtta aaagtggcac gagaaaagtt tggagaggaa
     1141 ggaattgggt atcatcggaa aagcgtagaa aaatataagt ccagtcgtag gtatgacttg
     1201 gttattgttt attctgcttt tcctcatttt tgggaccgtg atttagcgct tcagaattta
     1261 atttctcact taaaagaggg aggggagtta gctattgctc actctgaaag ccgggaaacc
     1321 attaatcaac ggcaccgagg agatggagct cctcaagctt ttctgcttcc cccaggagga
     1381 gagctggctc aggaactgaa gaaaagaggt ttagagataa cgagaattat tgataaccag
     1441 gagctttatc ttgtggtagg gaggaaatag ggggatcccc cctatttcca gaataatcca
     1501 caatgacaac gtccgttgcg ctctacgcta ccctgccttg tttctaaaca aggacatacg
     1561 ttttcgggaa tgttatctat tttacaaggg cagtaaaaat ctccatagcg ttcatatttt
     1621 ttcagcagtc cttctaccag gggctcttta agttcgttaa atttataacc tcgcagccgg
     1681 gcaaagtttt ccattctcgc tcttatttct tcttccggaa ttaagtcttt aggtcttcta
     1741 gctacccagg tattgttatc tttttccaga actaaaactg caccacatac tggacaaatt
     1801 atcctctttc ctacttcttt ttcttcccga agaataaaac ttacttgaca caccggacat
     1861 aatatttttt ctacctggtt cattctttga gagcctccga gatgcttaac tcatcatacc
     1921 ctacgattac cttatctcct atgactatca ctggaaaagc ccttcttcct gatatttttt
     1981 gcactttagc cactgttttt tctttctcct caggctctaa gaggtctaca tctacaaaat
     2041 cgtaggggat attgtgtttt tcaaagtatt ttttggtcat ctggcaaaaa gggcaagtgc
     2101 tcaaagcata aagcataact tttttcatga ttttcactcc tttttagaag attatagttc
     2161 aaaccggaaa ataagaaatt tattcttatt ctattcctca ttgaactatt ttatcccttt
     2221 tcagaaaaag aaaagggtga tactatttta tttgaaaagg aggattaata tgtttgatat
     2281 ccgggcagtg tctaccaata cttaccatgg ttgttctctt gaggaagcct taagcggtat
     2341 cgcaaaagct ggcttttcct gtgtagaatt agcttgcgta gaaggctgga ctaatcactt
     2401 tagtataatt aactaccggg aagaggattt aaagagtttg cgtagctctc tgtctcgttt
     2461 aggacttcga gtggtgagta tcagcgctca ttcggattta tctactcccg agggagtttc
     2521 ttacttagaa aaagctctgg accttgctcg aattctagga gcacaggtgg ttaatacggg
     2581 aactatagaa gaggaaaaga agaaaggtat tttgatttct aatttgcaag ttttagcacc
     2641 tgaggcagaa aaaaggggag tgaaaattgg tttagagatt catggaaatt ttttaaaaga
     2701 tggactcaga gctcgagaat taatggagga attaaatcat ccagcagtgg ggattaacta
     2761 tgatacgggg aatgccatat tttatggtgg tgtctgtcca gaggaggatg ttaaaaattg
     2821 ccttccctgg attgtccatt ttcatctcaa agataaaagg ggaggctata aagtatggga
     2881 ttttcctcct ttaggagaag gagatataga ttttggtaaa atatttgctc tggcaagaga
     2941 agaaaagata gaagttccct ggagtgtaga aattgagttt gatggtaagt ttcagcattc
     3001 ccgggagttt gtggaccagg cagtgaagaa atcttttgat tatctggaga atttgctgaa
     3061 gaagggaaag gagtgatttt atgatttact cgggagagaa agaacaaact cccttgatga
     3121 tttatagaaa tggggaaacg aaaaaagaaa ttgaagttcg aaaagaagac ccctattatc
     3181 tggagcttaa atattttctg gattgtgtag aaaataacaa acttgtagag aaggggagtc
     3241 cagagcaggc tattctggct ttagtcgctc gggattcggc agaaaaggga gagatggtga
     3301 agtttagccg ccagtaatga ggaagatact tttagtagaa aaaagtgctc tttggcgaga
     3361 gaaaataggt aattggttgc gagaagaggg ctattacctc attgccgtta gagaccggga
     3421 tgtggctatc aactgcctgg agaaagaaga aatagatata atactggtag aggaaaaagt
     3481 tttgcgagaa gatagtttct ctctgctttc ttttatccgt caatcttttc ccgatatggt
     3541 gataattgcc ttaggagaaa gcaatgattc ttttcctctt caggaattgt tttctaaggg
     3601 agtatatgat tatatttcta cctcttttgc tccttctcgt attctctcgg tgataaaaag
     3661 ggcggaagaa aaaatttctc tgttagagga aaataaagac ttaaagaaaa aggtagctgg
     3721 tcgtccttct tttgctggta tcaccggagt atcagagaaa atgcagcgag ttttcagttt
     3781 aattcttcag gtatcccagg tgaaaagacc aattcttttg gtgggagagc aagggacagg
     3841 taaagagctg gtagctcggg ctattcacca gtatgctttt tctgaggaaa aaccgttctt
     3901 taaagttctc tgtgccacct tgcctttaga gattttacct cgagaaaaac cagaagaggg
     3961 tactatttac tttgaagatg tgcacctttt accttataaa caacaggctg agatgttaga
     4021 gtttctggaa gaatatcggt tcggtaaaaa atcatcagat ttgcgaatta ttgcttcttc
     4081 cgaggttcct ttagaagaga aactggctga gggttctttc cgtgatgacc tttattattt
     4141 tctcaatgcg gttaaaattg aaattccccc cttgcgagag agaaaagaag atatcccctt
     4201 tttagtagat cgttttttac aagaaattga agaagaaacg ggaaaagagg aaatcagggt
     4261 ggataaagaa gcgctacgtt ttctcataga atatgactgg ccggggaatg tcatcgaatt
     4321 gcgcaatacc ttggaaggta tggttattct ttcctcttca gacttactta cctctgaaga
     4381 tttgcctgat tatattaaaa agagacaacg agaagagtta ctgggggtaa aagtggggat
     4441 gagcttagaa gaggtagaaa aaatcctcat tcaagaaact ttgaagttta ctcgcttcaa
     4501 taagaccaga gctgctcata ttttgggaat aagtctacgt actctttatc gtaaaatgga
     4561 acaatataat ttgagtctga gaaggaaata aatttttgtc attttgtcga aatattttac
     4621 tatttttgtc aaaaatctaa acatataaga atattttttg tcattttgtc aaaaaattta
     4681 ccttaattta tcttgccata ctcttgaaaa attgggttag aggaagtaaa atatgtagcg
     4741 gttaaattga agaaaggggt gatagtgaat gaagattaaa ccattaggag atcgcatatt
     4801 agtacaaaga gtggaagaag aagaagtgaa aaagggaggc attataatcc cggatacggc
     4861 gaaagagaaa cctcaacagg ggaaagtgat agcagtaggt acaggcaaag ttagcgaaga
     4921 tggtaagcgt atgcccttag aagttaaaga gggagataaa attctctttg gtaagtacgc
     4981 tggcacagag gtgaaaattg gggacgaaga atacctcatc atgcgtgaag atgacgtttt
     5041 aggtataatt gaaggttaag agaagggagg tatgattgat ggctaagcaa attttgtttg
     5101 atgaagaagc acgacagaaa gttttagggg gagttaaaac cctggctgag gctgtgaaag
     5161 ttaccctggg accccgggga agaaacgtaa tcatagagaa aaaattcggt tctcctctta
     5221 ttactaaaga tggggttaca gtggctaaag aaatcgaatt ggaagacccc aatgaaaacg
     5281 taggggctca actggttaaa gaagtagctt ctaaaaccag cgatatagct ggagatggaa
     5341 ctactactgc tacagtttta gctgaatata tatatagaga tggattacga gcggtaatag
     5401 ctggttctaa tcctatgctt cttaaacggg gcattgataa ggcagtagac gcagtaacta
     5461 agaagctaaa aagcataagc aaggaagtaa gtggtaagaa agaagttgct caagtagcaa
     5521 ctattgcttc taacaacgat gaatctattg gaaatatcat tgccgatgct atggagaaag
     5581 taggtaaaga tggagttatt actgttgaag aagctaaggg cttggaaacc actttggaag
     5641 tggtagaagg attgcagttt gataggggtt atctttctcc ttattttgta accgaccccg
     5701 ataggatgga agcagttctt gaagatacct acattttaat atacgagcag aaaatatcag
     5761 cggtaaaaga cttgcttccc attctggaaa aagtggtaca gagaggaaga ccgctgttga
     5821 ttattgctga agatgtagaa ggagaagctc tggctacttt ggtagttaat aagctcaaag
     5881 gagttattaa ctgtgtggca gtgaaagctc cgggctttgg tgatagaagg aaggaaatgt
     5941 tacaagacat agctgtggtt actggaggaa cattcatttc cagtgactta ggaataaaat
     6001 tggaaaatat tactcttgat gatttaggcc aagctagaaa agtggtagtg gataaagaga
     6061 ataccaccat tgtagaagga gccggtaaaa aagaagacat tatggctcgc atgaaccaga
     6121 ttaagaaaca aattgaagaa accacttctg actacgaccg agaaaaactg caggagcgtt
     6181 tagctaaaat tgctggtgga gtggcagtaa tccgggtagg cgcggcaact gaagcagaga
     6241 tgaaggaaaa gaaggctcga gtggaagatg ccctgaatgc tacaagagcg gcagtagaag
     6301 aagggatagt cccgggaggt ggagtagctc tgattcgttg cgagtcagct attaatgaac
     6361 tgagcttaga tggtgatgaa aaaatcggtg ctttagtagt gaaaaaatcc ttgagtgaac
     6421 cagcaaaaat gattgcccag aatgctggtg aagaaggctc agtaatagta gaaaagatta
     6481 aggcctccga taaccttaac tttggctata atgcgcttac cgggaaattt gtagatatgt
     6541 tagaagatgg ggtgattgac ccggcaaaag tagtacgtgt tgctctgcag aatgcatcta
     6601 gtgtagcggg cataatgctt actaccgaat cagtagttac tgaaatccca gaaaaagaga
     6661 aagttccagt agcgccacct atgcctgagt attgataggt gaggtataat agtaacggtg
     6721 aatgaaatca cccggtagag gtatctctac cgggtgattt accatctgga ggtgaaagcg
     6781 atgccgattt atgaatatca atgcactcag tgcggtaaaa cttttgaaga gcttcaatct
     6841 tttagtgata agccagtgac tcaatgtcct ttttgcaaag gtagagtgaa aaaactgatc
     6901 gggagaaatg taggcctggt gtttaagggg agtggctttt attgtactga ttaccgttct
     6961 tctggctctt ctagttctac gaactcttct agttgcagta cttgctcgag tagtaactgc
     7021 tccacttgta gtggaggaag cagttctcag taaagggcat ggacctttgg ggataccttt
     7081 taattgctgg attattggtt ttttttgctt ccttgcgggg ggtaaaaaga aggggttctt
     7141 cagtttcagt atctgaattc tggcaagaaa aggagaaaga atttggagaa aagaaaattc
     7201 tttctggatt ttctcgctat ttgggaggcc atcctcgttt tgaagcgata agtgatggcc
     7261 tccttttttt aatgtccaga agtttgtggt ttgaaaactt tgaaaaaggt cccagtattt
     7321 ttggtattgc tcctcctttt aaaaaagtaa tttttcgtat ccttctctcg caaatagaag
     7381 atgtagaatc tcttccggaa aaagagttgc taaaaagagg ttttgatagc cgccagttat
     7441 ggaaatacca tcgtctatct cgtcgtcctt tttatcttgc tgttaaatat agagatgaat
     7501 gggaaaaaga gaaaactctc tactttgata gtatgattga tgcagatatt tggagagagg
     7561 agctggaaaa ggctaaaagt aattatgttc cagaagaagg aataaaagaa agcggggttt
     7621 gccctcagtg tgggaagaag atagaccccg attttaaact ttgtccttat tgtggatgtg
     7681 gactttctta aggaggtagg atagatgtct tttcaagtag gacactacca ggatttttct
     7741 aaagctacag ggtgtacagt ttttttgttt gaagagccca atcgagcggt ggccgcagtg
     7801 cgaggaggag ctccaggagg tcgagagatt tctactcttt ttcccggaaa cctggtagag
     7861 ggagtggatg ctgtattctt tagtggaggt agcgctttag gtctttcctg cggagaagga
     7921 gtggtagatt ttttgcggga aagaaagaag ggatttaaga ccccgggggg aatagttcct
     7981 attgtagctg gggcagtgat ttatgattta gaagtgggag agccatattc tcctgataga
     8041 gaatggggat ataaagctgc tctatctata gaagaagtaa taaaagaggg aactatcgga
     8101 gcagggacag gagctacggt ggggaaactt atgggaatgg gaaaagcagt aaagggggga
     8161 ctgggaagag gaaaggcaaa agcaggagga gaggaaatat ctgcctttgt agttacgaac
     8221 tctctgggaa atatttatca tcctcaaact ggagaattgg tggcaggggt taaaatagaa
     8281 agaaaaggag cagcaatttc tccttttaat actactttga ctttggtggt ttttgattgt
     8341 tttctctccc gagaagaatt attttctctt atgggggtag ttcattctgc tctggcatct
     8401 tgtattcgcc cttttgccac tctttacgat ggcgatacca tttttctcgt atctacaggg
     8461 aaaaagagag taaatgatta tttggctcta gtctcaggaa tttatgaaag tgtgggagag
     8521 gcggttttaa attcagttac caaagctacg ggactggctg gaattctggc taaaaatgat
     8581 ataatagttt aagtataccg ttgaccacgg gcagcgagtg agtttgatta gtagtatttt
     8641 cccgtctggt atctaaaaag aactggaacg gaggtgcttt ctttggaaag aagaactaag
     8701 aaatttgttc tggctggtgt tttgggggcg gtttcggtga ttttgagtct tactccttta
     8761 ggacttattc ccgttcccaa tcttgcttct tatgctacca ctatgcatat tcctgctata
     8821 atagggggta tcataggtgg tccagaggtg ggggctttag tggggttgat tttagctttt
     8881 tccactctcc acctgtttgc gggtaatatt ttagcttgtt tcttgcctcg tcttcttatt
     8941 ggagtggtag cttattatct ctggcaatgg tcagggagac aagactgggg aatagttctg
     9001 ggaagtttag gaggaacttt taccaatact ataggagtat taagtttaat ggtagtgacc
     9061 ggttatttca cctgggcgca ggttttacct gtttttgttc ttaacggagt tttagaatta
     9121 attatttctg gtattttagt gttacccctg gtacgtattt taaagcgggt ggtgaaattt
     9181 gagtagaata ttggtcatcg atgttggaaa tacccatact acctggggag tatatgagga
     9241 taagatactc ctttcacact ggcgagtatc cacctcctcc caccgtacct ctgatgaatg
     9301 gagctttgta tttcgaaatc tattggaaga ggaaggaata aagagagcta cgctataccg
     9361 ggtagttgtt tcctgtgtgg ttccgccggt tttaggtgct cttgaatatc ttttccggga
     9421 gaaatggcgt cttccttatt tatctgtggg gccaggaatt aagactggtc tatctcttcg
     9481 ggtggaagct aaagaggtgg gagcagaccg ggtagttaac gctctggctg cttcttctct
     9541 ttatggagga gatttaatta tcgtagattt tggtaccgct actacttttt gtgcggtaac
     9601 gaaaaataaa gaatatttag ggggagtaat tgctccgggg ataagcatat cggctgaagc
     9661 actttacgaa aaaaccgcta aacttccccg ggtggatatt gaaattcctt ctcatattgt
     9721 gggaaagaat acggttgaag ctatgcactc cggggtgttc tttggtcata ttggtttatc
     9781 ccgggagatt gtgaaaagga tgaaagaaga attttcttta gaggcaaaag taataggtac
     9841 aggagggtgg agtatttttc tacaccgcta ttgtgatttc attgatattt ttgacccggt
     9901 tttgactttg gaaggtttga ggattattta tgagctcaac caagaatgaa gaacaagaat
     9961 tagccattgt aggagggcag tctaaaatta gcggtagttt tgagggaggg ggatttttgg
    10021 tggtggaagg agagtttgct ggttcaatcc agtgtcggga gctagtgata acgagaaaag
    10081 ggaagataga ggcttgggta gaagtggaga atatggaagt atggggcgaa gttaatggtt
    10141 tctcccgagc gaagaaagca atttgccgca aaacctctcg cataggaggt tgtattatgg
    10201 gaagcggtat tgctctagag ccaggagcaa taattgaagg agctctgacg ctggagaagg
    10261 tggaggagat agagggtgaa ggaaaagaga gtagttagcg cgagcttagg ttcctcaaaa
    10321 agaaatcatc gtgcggaaat agagcttttg ggatgcaaag tctttataga gagaatagga
    10381 gtagatggag ataaaaataa gtttgtggag ctattgcaga gcttagatgg taaggtagat
    10441 gctattggtt tagggggagc agacctttac ctgcgggcag gaaagtaccg ctatgaagta
    10501 gaagatgtag cgcgcctggt atcggtcctt aaagaaacac ccattgtaga tggaggagag
    10561 ttaaaagaaa cctgggagcg agaagttccg cgttgtttac gagaggaaga ggagatagaa
    10621 cttaaaggga aaaccgcttt gataatgagc gggatggacc gttatggatt ggctgaagga
    10681 ctttatcagg aaggatgtca tcttctcatt ggagatttac cttttgctgt gggtgttcct
    10741 ggagctttac gaaggttggg gacccttcgc tttttgtcgg tgttgatgat gcctgttttg
    10801 cgccgtcttc ccattgatgt tttgtatcct acaggaaaaa gacaagaaga gcgtaaacct
    10861 cgttttagtg gttattttga aaaagcagat ataattggag gagattttct ctatatctgg
    10921 cgttttatgc ctgaccgttt accgggtaaa atgataatta ctaataccgt tacttcgcaa
    10981 aacatatcgg agatgaaaga aagaggaata aagattctcg ttaccactac cccggagata
    11041 aataatcgtt cctttggtgc caatgtttta caggctatat ttgttgctgt tctggaaaga
    11101 aaaccggaag atataaagcc tgaagaatat ttagacttga tgaaagagtt gcatattaaa
    11161 ccccgagtga taaaattata gctcatcttt ataaaaaaaa taaaaagata tacaatattg
    11221 aagctatgga agaaaaagtt ttgactataa gaaaagcaca gttagaggac cttcaacaga
    11281 ttaccgaaat ttataatcag gcggtattaa aaactatttc tacttttcat ctctatcctc
    11341 gttcctggga agagcaggta aactggttta aaagtcatgg agataaatat ccccttttag
    11401 tagcagaaga aagtggttct gtgatagcct gggcgtgctt gagcccctat tctgacagag
    11461 agggttacca gtatacagtt actgattcca tatatgtgag agaggaattc cgtcgccagc
    11521 atattggtta tgagcttttg aatattctta taggagaggc tcagaaattg ggttatcatt
    11581 cagttatagc ttttattgcc cgggaaaatt tagccagtat cgggcttcat gaaaaagtgg
    11641 gttttgtata tcgaggggaa ttgaaagaag caggtttcaa atttggtagg tggatggatg
    11701 tgtgtttcta tcagagaatg ttggaaaggg gggagagaag atgaaaaata gagagatttt
    11761 agaaaaaaag tttcctaaaa aagaggtaaa aactcgtccg ggtagagaag ggcgagaggt
    11821 ttattacctg gaaacagctt cggtgattca gaggttgaac gaggcttggg aaggcgattg
    11881 gtcttttgag gtaaaagata aaggcataga caaagaagca ggttatgtgt gggttctggg
    11941 gaggttgact tgtggagaag tgataaagga acagtttggt tctaaaatta tttctctgag
    12001 ttctgatgga actatggttg atctgggtga tgacctgaaa gcagcagcaa gtgatgcact
    12061 gaaaaaatgt gccacccttc tgggagtggg gctttatctt tatcaggagg aggaagaaga
    12121 aactttcccc cgtcctgctt ctcagaggca aaaggacttt ttgcgcaatc ttttgaaaga
    12181 aagaggtaag gtagttgatg aagagatgct tgagactatc tcttctgtgg aagcttctcg
    12241 tttgattgag gagctgaaag aaaaaagacc ataaagatat ggaggagggt ttactatgaa
    12301 cttttggggt aagtttttca cttttattgt aggaataatt ctgataggta aggctttggg
    12361 aatagtggct atgactttca agctggtacc tcttcttacc cttcaagaat gggtggtggc
    12421 cacttatttg tttttcagtc aggatatttt ctcccagatt ttaggagtta tactggctgt
    12481 tatccttgtt tttgtggctt ttttagtttt tcggctgtta atatctaaac cggagaaaac
    12541 cctcgtatat catactgaac aaggagacat aaaggtttct tttagttcgt tgcagtcttt
    12601 agctcaggaa gctctgcgga gttttgatga aattttgcag tctcgggcgg agatagagaa
    12661 ggtagggaat gaagcccgtt tgattcttca tttggcagta aaaccagaag cttctatccc
    12721 tgaactttcg ccagctgttc aaaataaagt gaaagagaaa attgaacgcc agactggtgt
    12781 aactgttaaa gaagttaagt tgactattga cttaaaaccc gaggaagagg cggtctaata
    12841 tttttaaaaa cccgaaaccc cggagataaa agaaaaggac agcagctccc agggtgaaaa
    12901 gggtagttgt ttcttctttc ccgattactc tccaggaaaa tgttccattc ctatcgggat
    12961 aaggtgaaaa acgggggaag aaacggggta ctctccggat ataagcttta taggtttccc
    13021 cgaaattgtt ttctaaaaat ttttcctcag cctggataat cgggaaatat agaagataaa
    13081 aagcaaacca gaatagaatt attgaccacc ataaaccaga gatgatggct ataccagttc
    13141 caattatacc attagctaaa tagagagggt tacgcaggta agcatatggg cctgcagtgg
    13201 ttaacctttc tacttcttgc ataggtccac ggtaattttt gatataaccc actccccaga
    13261 ttctcagccc ttctcccagg cacgttagag ctatccccca gctaaataaa ggatgatgag
    13321 gatgggcaaa aagaaaaatt atcaacccta acgcccccca gatgatgccc cggtggggga
    13381 aaagtttggc accccatttt tctaagctga ttgcattatt cgccatagct catcctgagg
    13441 gcattccttt atttcttctt tttccccatt ttggtggatt aaaatcctgg aggggtcttt
    13501 ttttactctt cctattactt ctgccggcac aacttcccga agagcctgaa gaagaggtag
    13561 aggcgcaggg gtgaaaatta taagagtgcc acttgaaatt agtttcagag ggtctaaatt
    13621 caggagttga gctaaaactc ttgtttgagg ataaaccagg attttttctt cttctatttc
    13681 aaatcctaaa cccttactgg tgcaagcttc ccagatagct cctaaaactc ctccttctgt
    13741 ggcatcatga agagcgtgag gatgaaaagg aagagcaatt tttccttcct ggaaaacgct
    13801 catcattttc cagaattctt ttcctttatt tattgtttcc tcatccagga gatgttgcca
    13861 ttctttccct ttttcggaaa ataaaatagc tgtaccttca attcctgctc ctttggtcat
    13921 cacaatatag tcgccggctt ctattttgct tacatcggga agatggttat cttttaccca
    13981 tcccagccct gtacaatgaa tgatagacct gtttactgct tctgatattt ctgtatggcc
    14041 tcctataatg ctgactccca attccttccc tacttcatct aactccctca ttattatttc
    14101 tatttctttt tctgaaattc cgggagggaa aataagagta actagaagag ccacaggttc
    14161 ggctccagaa caaaccaggt cattcaccac tacttgagtg gctaaaagtc ctaagtgaaa
    14221 tgaagaacca gttatggggt cgcaggtaag agatatcagc tctttcccta attgtactat
    14281 tccacagtct cttcctaatt gggctcccca gattacttcc tttctcttct ttccctgaaa
    14341 agggaaaact aatctctcca gaactgaagg agggatttta cccacttttt ccatcaccgt
    14401 gatttcttcc tttctctatt aataatttgc ctttcatcca ggctccctgg tctacaaata
    14461 gtatctcagt ttttatctct ccttctactt ttccagaaga agaaagataa aaaaaggaag
    14521 aattaacaat gcctcgtaaa aaaccttcca catagacgca ttgtgcttcc acctcaactg
    14581 ttgcccgagc gttttccgct ataactataa tcgggcactt taccttacct tttaaaatcc
    14641 cttccactcg cacaagaccg cgggtttcta tatttccctc cagggttact aaaggaggga
    14701 gaaaaacttc taccggacac tcccgcaaaa aacggtttaa aaaatcatga cgggacttaa
    14761 aagataaaga tttgctccac ttgtctaaag attctaactc acgcatttgt aagtattata
    14821 tcagaataac agatagcaac taacttgctg attttctaat ttatattatt tttgttcccc
    14881 agtttgagtt tcatctatag ttaagcgcaa attatggtaa tgcctcttct tagtattctg
    14941 gttctctcat taagataact catttataag ttatttaata ccctattaat atacaataac
    15001 agacacgagg attttcttct cccgtcattc ccgagcactt ttatcgggaa tccaaaggtt
    15061 tttactgaag aaagtctttt tctttcctca acgttggagc gctttttccc cagcgtaatt
    15121 attctaaatc ttttcccatc ttttgtgttt tttatcttac atcgtagata attctctttt
    15181 tttcgggggg ggttataaga gggtatgaag aggtgctttt tttataactt tgacagttat
    15241 aatccataaa atattgatat attaaaggaa taaaagcctc cttttttatt tactccccta
    15301 cagtatcact gaggataaac ttaggaatat ttatccttaa agcttggatt ttcaggaaaa
    15361 aactactttc tctttgaatt ttatgttata attctcttct gcggggcgta gcgcagcctg
    15421 gttagcgcac ctgccttggg agcagggggt cggccgttca aatcggctcg ccccgaccag
    15481 aattataatg ctcgcaggaa gcgcattcca aatagagcag cttttcaccg gaagcctggt
    15541 ttttggtgag tca
//
LOCUS       DAOSVE010000033        15282 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_2379_length_15282_cov_72.015564, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000033 DAOSVE010000000
VERSION     DAOSVE010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 15282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15282
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2379_length_15282_cov_72.015564"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..275
                     /locus_tag="PK016_04215"
     CDS             51..275
                     /locus_tag="PK016_04215"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HXL02308.1"
                     /translation="MGNEVILSARVPEDLVKKLEDLAKKTDRTKSYLVRKALEEYLEE
                     LEDYLIAFERYTSGDKEYLTTDEVKHYLDI"
     gene            265..537
                     /locus_tag="PK016_04220"
     CDS             265..537
                     /locus_tag="PK016_04220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HXL02309.1"
                     /translation="MTSEKWKVLWEKRALEDLLPIARKDARLIVEKVENHLSKDPLKI
                     GKPLKGQLKTFYRYRVGNYRVIYAVEKKEVTILVVRVGKRDEIYKK"
     gene            1118..1336
                     /locus_tag="PK016_04225"
     CDS             1118..1336
                     /locus_tag="PK016_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016872433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HXL02310.1"
                     /translation="MKNLVAYIEWDPETKLYVGIVPGIPGVHTQGATWDELQKNLKEV
                     LELCLEEDPDLKEDLPRFVGLQEIEVGE"
     gene            1333..1566
                     /locus_tag="PK016_04230"
     CDS             1333..1566
                     /locus_tag="PK016_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014933300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HXL02311.1"
                     /translation="MSKLPLVDAKTMENLLLKLGFEKVRQKGSHVFYRHIDGRTTTIP
                     HHPGRKLARPLIREILREIKLSPEQFNEELRKL"
     gene            2104..3270
                     /locus_tag="PK016_04235"
     CDS             2104..3270
                     /locus_tag="PK016_04235"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXL02312.1"
                     /translation="MVKFRELTGKEEHKKCKGGIAMISFRSARPEEVGQILDLVNYVF
                     RTLSGLEPSMGRQFPTLICAENAPDLYVAVDDGRIVAHIGIKKNIVMIYGHKVNVANM
                     GAVCTHPDYQGRGIGTSLLHEVFKRLDEEGIGLVIISGARGLYKRNSCVKIGGAATFV
                     LEKNKYSAGDAEGMHYYISRDGNGATDLFDIYRWEAVRYQRARLEFPVLLKAVPMVPP
                     PVLKLTMVVALTEGKISEKLAYLIGYEENPGMFKVVEYAGERKAIAWLLERLLNDEGM
                     TQVVLELPTYDRILTSILNSRGLQGILSYYPSTTVRVINRESLWRDIYPIINETWTVD
                     TPPSSLAELPDRVVDDFAALARFLFADFNRPSYGEPWDQVFPLPLPWPNGLNYI"
     gene            complement(3408..4187)
                     /locus_tag="PK016_04240"
     CDS             complement(3408..4187)
                     /locus_tag="PK016_04240"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HXL02313.1"
                     /translation="MPPKSSIGALEQAIKILEIVVFEGRNGITIKEISRKVDLHPSTI
                     YRYLNTFLSYGYVSRSTDGNYKPGIKLIELGNYVLNGFDLREIAHPYLVRLVNEVNQT
                     AHLAIREGNEAIYIDKVEGPKTLQMRSRVGMKIPLYCTALGKVLLAYSSEEDIDHYLK
                     EVELAPRTQNTIISSCELKEELLNIRKQGYALDNQENEQGIICVGAPIFDFTGQVVAA
                     LSISGFFKDFSPDKIPEISSSLQRTVRSISQDLGGPGKQAG"
     gene            complement(4187..5152)
                     /locus_tag="PK016_04245"
     CDS             complement(4187..5152)
                     /locus_tag="PK016_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HXL02314.1"
                     /translation="MKVDVLSYGELLIRLTPERFKTFLKSDSWEVEIGGAEANVLAGC
                     SILGLKTQLLGALPDNFLGRRVFQELRRFGIGVDHLELTEEGRMGLYFLEFAHRREGA
                     EVLYDRKDSTFSHFELREEDFSLLSQSSLLHLTGITPPLSPTCDMNVRNLLKFKPATL
                     QISFDVNYRSKLWSPEECRKFMEEIMKSIDIIFIKEEDLQVIFGLENEDYWEKLAFLQ
                     SNYGEDKIYVLTAGEKGCFVLSKDVSIHQSSFATEAVDRIGAGDAFVAGFLYGYLSKK
                     NLGEAALWGNAMASLKMNVVGDMPVFDRHFVEALVTSSISGKVNR"
     gene            complement(5291..7108)
                     /locus_tag="PK016_04250"
     CDS             complement(5291..7108)
                     /locus_tag="PK016_04250"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HXL02315.1"
                     /translation="MKKTKADVLVVGSGGAGLRAALSAKETNKSLDVKIVTKGELLKD
                     SVTATSYSDRMAFHATLDTTEPYSEDSWRYHAEDIFVIGGMVSDQNLAEVLAKNSKDA
                     FEYLDNLGVPFAREGNRVKQFRTDGSKYARACFTGPDTAVQISKALAKKVKEENIEVI
                     ENTMIQGLLLDEENRVKGAWGLQILDNEYFFVDAPAIIMATGGAGQVFMDNLFPPLAT
                     GDGWGAALRAGAELVNMEFIQIGLSSKKTKIACSGSFMRSLPEIVNSEGEDVLLKYYK
                     DKYSQKELVNILFSKGWSWPLSCEEESHIIDVAVAKEIQQGRRVFLNYAQNPNFLEKE
                     SLPEKVLEWYRNSLDEDLLRSDLFASPLLRLKKMNREIYELLKSRGINLDEEGKIEIV
                     PAVQHFQGGIKINEKAETTIGGLYACGEAAGGQHGANRPGGNSLLDTQVFGKISGQEA
                     AIYSSSIKQEGITEEETEKIKRQFPKFSREVGGSNQVRHHIREIMTRNAGVIRVPHNL
                     KAAYEEICSIREEEILHASESLKDYLETLNIHLLSRLLLQTMYERKESRGPHLLFKDE
                     NSMELYPRDDQSWAYHYIVAKLKDGAITLEKRSVNRRNL"
     gene            complement(7113..8432)
                     /locus_tag="PK016_04255"
     CDS             complement(7113..8432)
                     /locus_tag="PK016_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HXL02316.1"
                     /translation="MNDHKRFKYSTMDELIADIKKFNLDIPFSYNLDLLREKIELNGK
                     TIGNRLAIHPMEGCDSTPDGAPGELTTRRYMRYTRGKAGLIWFEATAINEKARANDQQ
                     LFLNERTVSDFSLLINRMREEADEEGNHSPYLVLQLSHAGRFGDHRIIALHDEKLDKL
                     AKVEPHHPVISDRELEELGNNYVNAAQWAKQAGFDAVDVKSCHRYLLSELLGAREREG
                     KFGGSYENRTLLIKEAVARIKKEAGIEVAIRLNISDFLEYPISWGVNEKGELDLSEPL
                     KLIGELRDMGVKLINVTAGSPYINAYISRPADGEARKYTPPEYPLLGVERLIKLSREV
                     QENFKDIIVVATGLSWLRQFAPYVASGMVEKGYAKIIGFGRMAFAYPDFAKDILQNKG
                     MDKRKVCITCIRCAELKAHRKITGCVIRDGEVYLKPYREIEKKRDQE"
     gene            complement(8457..9254)
                     /locus_tag="PK016_04260"
     CDS             complement(8457..9254)
                     /locus_tag="PK016_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008192020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXL02317.1"
                     /translation="MKKVIIIVLAVLFMIPVFWTITSSLKSMNEIYKYPPSIFPEKIS
                     LEGYANVIEQSDFPRYLANTLFVAIISTIITVFLCVSLGYGLAKGTFKYRGFLTEVVI
                     VTLFVTAQVIMVPLFVIIRQLGMMDSLWGLIIPAVFTPTGAFTAIQYMRGIPNEFLES
                     AKVDGAGEWQIFLRIVLPLSKPLIASLSIFSFTWRWNDFILPLIVINSGKKFTIQLAL
                     ATLQGQYGIPWNEILAFSVMAIIPTLVIFLLFQNLFMKGISAGGLKY"
     gene            complement(9251..10129)
                     /locus_tag="PK016_04265"
     CDS             complement(9251..10129)
                     /locus_tag="PK016_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012310592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXL02318.1"
                     /translation="MTKCGIGENRFAFVIPGFIFHFLFYLLPILLMIILGFFSWDILS
                     PMRFVGLKNFSDLMKDKWFWNSVVVSFKYTFLAVPIIFVISLLLGLLLQEEDTFSKVM
                     RVFFYWPYMIPMVAAGTMWKWLFSRNFGLVNHILAQLGLQPVSWLDNPTLALLTVVVV
                     QVWVLAGFMMMIFIVGLQAISEEFYEAASIDGATSFQKFWYITLPLLKNTNISVITLS
                     IANCFRNFTIVYIMTTGGPGYATTVAPLYVYQKAFTDFRIGYASAGSLVMLLITFVIA
                     FAVRKAQEGGEREGTA"
     gene            complement(10209..11477)
                     /locus_tag="PK016_04270"
     CDS             complement(10209..11477)
                     /locus_tag="PK016_04270"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL02319.1"
                     /translation="MKKVLLFTTLALLVVLTFGSAFAVELTMYSSDESKARVLRQVVE
                     LYQEEHPDVKIEVVAFPYANYMQKMSLVFLGGEPPDLLETTATYLPQMTQFLRNVGPD
                     IEENLGLSPQEYKDSTYDVVKVYLGEGDIVHAVPLHFTTYSVWINKEMFEKAGIAYPP
                     KGGRTEPWTWEEFKDVLRKVKSENATPYAMSIEYSADKFFSYLALWNIKILDEEGNFV
                     MDQYENAEKAIDEFVGLFKEELVPPAEWLSGESHTSNFFGGITAVDWAGSWMARESFN
                     AEKQNNVAPCYVPVIEDWFGISGGGFLAAPQTGNAEKEKAATDFVMWIADKDKGYTEY
                     IKRSRDLTAYKNHSIDYGEPLLDEWAEVHQVLAERAPAWSTEVRATAVWSRLTDTIRK
                     DLALGISESMTAQEMIQHWKEEAQRILSEL"
     gene            complement(11602..12597)
                     /locus_tag="PK016_04275"
     CDS             complement(11602..12597)
                     /locus_tag="PK016_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013412631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXL02320.1"
                     /translation="MKLCIVGSSGHYQYVLSPVKCNRNLDLEIAGIAPGSEGENMEDL
                     YQEVINLGYKPLIYEDYRNMFDEINPDIAVINCYFGDLAKVSKEALSRNINVFTEKPV
                     ATTLEDLESLKKAYQRSKKTFVAMLGIRYKPWFLTAHHYVEEGSIGKVRLLNAQKSYK
                     LGRRGENYKSREVYGGTIPWVGSHGVDWIRWFSREEFVSVYASHSSKDNQDHGDLEMT
                     ALCHFVLTNEVFASLTIDYFRPANAPTHDDDQLRVVGSEGIIEVIKNKVFLIDSKIAG
                     VREVPLEPEGSIFLDFLKEVEGRGKCLVTAEDSFRITEVCLKARQSADEAKVIYL"
     gene            complement(12599..13849)
                     /locus_tag="PK016_04280"
     CDS             complement(12599..13849)
                     /locus_tag="PK016_04280"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXL02321.1"
                     /translation="MKEVFFAVVGVRNFAKEHINFIQQLEKEENIKLGAIVVENKRKN
                     FELWKEFKREGIEIYDSFSELLKKGKKLIDVITLPTAIHTHAPMAIRAMETGYNVLLE
                     KPPAPTIQELDKIIETEKKTRTFCSVGFHMIHSRTIRKLKEIILQGELGEIKEISCKA
                     CWPRFKSYYTRNEWAGKTIYDGHIVLDGPMHNALAHYLNNMLYLLGPSMNEAVNLKTV
                     RAEFYRAHTYIEAEDTSCLEVEAVNGAKVYFYVTHASERTRDPYMEITGTKGRAFWEF
                     SEKTKVVLNRGEVLEFDNQGVNPHLEVFRVTAQKHLGLIEEAWSTPANSRPFVVAING
                     AYESAGKIVPVPRELVREFETEYGEYKTVLEGIDEIIDEAFEKRALFSDLGIKWAIPS
                     TEVNVEAYKAFNPFSLNNSRERIV"
     gene            complement(13873..>15282)
                     /locus_tag="PK016_04285"
     CDS             complement(13873..>15282)
                     /locus_tag="PK016_04285"
                     /inference="COORDINATES: protein motif:HMM:NF012754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02322.1"
                     /translation="RLTDQKLVFYNVYRAIIMHIMQRSDIMKPLGRIVLVFLVVMGIA
                     GISWATELPPGISRELLSLGRETLKPGDGWASYGEGTSGGSQASVENIYLVRTKEELI
                     EALGGDREKNMEDDIPRIIFIEGTIEISEPYETYKDPEFDFDKYLEEYAPEKWGKEKE
                     VSGPLEEARARSQKNQADDILIYIGSNKTIIGVGKDAKIVGATVKLKNAHNVIIRNLT
                     FESPIDYFPQWDPTDGEFGEWNSEYDCVSIEGSKNIWIDHCSFSDGRYLDNEAPTYFG
                     RLFQQHDGLLDITKESDLVTVSYNVFYNHDKTMLIGSSDSNVSDRGHLRVTLHHNLFE
                     NVTQRLPRVRFGEVHVYNNYYKINSHSPYPFDYAWGVGVESKIYAQNNYFDLPWTVPA
                     SGIIRRWKGSCIYEEGTVISYPSSQPFYANLLKAHNDVNVNDQLEPSVGWKPTLFLKI
                     DPAISVPAIVTSEAGAGKL"
BASE COUNT         4328 a         3314 c         2841 g         4799 t
ORIGIN      
        1 aataattaca ttgtaataca atatcataca ctcagaaagg aggaaaagtg atgggaaatg
       61 aagtaattct atctgcccgt gttcctgaag atttagttaa aaagttagaa gacctggcta
      121 agaaaactga cagaaccaaa agctatctgg tgagaaaagc cttagaggaa tatctggaag
      181 agttagaaga ttatttaata gcctttgaaa gatatacgag cggagacaaa gaatatttga
      241 ctactgatga ggtaaagcat taccttgaca tctgaaaagt ggaaggtgct ctgggaaaaa
      301 agagcacttg aggacctgtt accgattgcc agaaaagatg cccgattgat tgtggaaaaa
      361 gtagaaaatc acctctcaaa agaccctctc aagataggaa aacctctaaa aggtcagctt
      421 aaaacttttt acaggtacag agtggggaat tacagggtaa tttatgccgt agaaaagaaa
      481 gaagtcacga ttttggtagt gcgggtgggg aaaagagatg aaatttataa gaaataagct
      541 gctttcatat ataatgtggt ccttgcggga tttctttccg aaagaaaaga attgtcatta
      601 cgagatacga caaaacggcc ctcgtgatga gagaaaacac cctcacccta accctctccc
      661 ttcaaggttt ccttttctgt catgcctgag tgcttttatc aggcatccac tgatggatgc
      721 ttttcccacc agtgattttt agttttgctt tttgtgtgtt ttctttgtat ttattttgct
      781 ttttgggggc gataagaggt ttttaccccc cttattgttc tgtttttctt tcctcagtag
      841 tcttttgcct tttatgtttt taatggggga ttataaaggg gtgctcccct ttattgtcga
      901 tagaaccatt cagggatgat agagaagagc aacatatgta atggttgaat ggattgtgat
      961 aaaataaagc gggaactggg ctggtgaata tcaatagctc tcttttaaga gccctagggg
     1021 cgaagctgca gggatgtaga aaatctttta atccactaaa gctacattcc gttggctcac
     1081 taaggcagga agggtgtatt tctataggag gtgaagcatg aaaaaccttg tggcttatat
     1141 agagtgggat cctgaaacaa agttgtatgt tggaattgtc cctggtattc cgggagtgca
     1201 tactcaaggg gcgacctggg atgaactcca aaaaaactta aaagaggtgc tcgagctctg
     1261 tttggaagaa gacccggatt tgaaggagga tttgccacgt tttgtgggct tacaggagat
     1321 tgaggtgggt gagtgagtaa gcttcccctg gtcgatgcca aaacgatgga gaatctgctt
     1381 ttaaagctgg gatttgaaaa ggttcgtcaa aaaggaagcc atgtctttta ccgacatatc
     1441 gacgggcgga caaccacaat tcctcatcat ccaggaagaa aattagcgag gcctcttatt
     1501 cgagagatat tacgtgaaat taaactgtct cctgaacagt ttaatgaaga gctgaggaag
     1561 ctgtgaaggc cttcgactgt ctttcgcttc ggtatcatat caggattagc gaataatctg
     1621 tttgtgaagg gaaaaaagga gtaaggggag ggggaaagaa gaagaaatta aaggagagga
     1681 aaaaagaaag ggggttgtga aggggtcttt tccccctcat agtcattaag tattacgtcg
     1741 taggctgtct gttttttctc cctcagtgga aaaaagtccc caacgtagcg accttgagtt
     1801 tttgactttc gatataattt actttttaaa gtttttaaca ttttaatcag ttttgcagga
     1861 tactataatc tccggaaatc aaaagacaaa cataaaagta aaagactact gataatcatt
     1921 gcatccctct ttgcaagctc ttctcttttg ctttttagac ttgtgctgga tactcaacag
     1981 agaagataat agaggcattt ccgcgctttt cttatatcgt tgtgagcttg gtatcctgta
     2041 acaatcttac ctaaatagtt atttcagaat tgagtagtgg cccatagaca aaaagggctg
     2101 ttaatggtta aatttaggga gttaacaggt aaagaagagc acaaaaaatg taagggggga
     2161 attgctatga taagtttccg ttccgccaga ccagaagaag tgggacagat tttagatttg
     2221 gttaactatg tatttcgtac tttgagtgga ctggagcctt ctatgggaag gcagtttcct
     2281 acccttatat gtgctgagaa tgctcctgac ctttatgtag ctgttgatga tggaagaatc
     2341 gtagctcata ttggaataaa gaagaatata gtaatgatat atggccacaa ggttaatgtg
     2401 gcaaatatgg gagcagtgtg cactcacccc gactaccagg ggcggggaat tggaaccagc
     2461 cttctccatg aagtgtttaa acgattagat gaagaaggta tcgggctggt tatcatctct
     2521 ggagcaagag gtctatataa aaggaattcc tgtgtgaaga taggtggtgc agctacattt
     2581 gtacttgaga aaaataaata ttctgctggt gatgcagaag gaatgcatta ctatatttcc
     2641 agagatggaa atggtgctac cgatcttttt gacatataca ggtgggaggc ggtcagatat
     2701 cagcgagcta gactggaatt tccagtgctg cttaaagcag ttccaatggt acctcctcct
     2761 gtgctgaaat tgaccatggt agtagctttg actgaaggga aaattagtga gaaacttgct
     2821 taccttatag gatatgaaga aaatccgggg atgtttaaag ttgtagaata tgcaggagag
     2881 agaaaagcta ttgcctggtt actggaaaga ttattgaatg atgaaggaat gacccaggtg
     2941 gttctggaat tacccactta cgacaggata cttacatcaa ttttgaattc gagagggtta
     3001 caggggattt tatcttatta cccttccact accgtaagag ttattaatcg ggaaagtttg
     3061 tggagagata tatatcctat tatcaatgaa acatggacgg tagacactcc tccttcttct
     3121 ttagctgagc tgccagatag agtagttgat gattttgcag cactggcaag gtttctattt
     3181 gctgatttca atcggccttc atatggtgaa ccgtgggatc aggtttttcc gctaccctta
     3241 ccctggccta acggtttaaa ctatatataa aaaagttatt ctcccgaata actggatagt
     3301 gttccactct ttatgagcct cagtacagat ttattttctt tcttcttgaa cttctgtatt
     3361 tgggtttgaa agctccataa ttaactctca ataaggggga gactggttta accagcttgt
     3421 tttccagggc ctcccagatc ttgagaaatt gaacggactg ttctttgaag tgaagatgat
     3481 atctcaggga ttttatccgg agagaaatcc ttaaaaaacc ctgaaatgct gagtgcagct
     3541 accacttgcc cggtaaaatc aaaaattggt gctcccacac agattatacc ttgctcattc
     3601 tcctggttat cgagggcata gccctgtttt ctaatattca gcaattcctc tttgagctcg
     3661 caagaggaga tgatggtatt ttgagttcga ggagccaact ccacttcttt aaggtagtgg
     3721 tcaatgtctt cttcggagga ataagccaga agaacttttc ctaaagcagt acaatataga
     3781 ggaattttca tccccactcg ggagcgcatt tgcagggtct tgggtccttc cactttatca
     3841 atataaatgg cttcgttccc ttcccggatc gctaaatggg cggtttggtt tacttcattc
     3901 accaacctaa cgagatatgg gtgagcaatt tctcttaaat caaacccatt tagaacgtag
     3961 ttacctaatt ctatcaactt gattcctggc ttatagttac catcagtact tctagaaaca
     4021 taaccataac tcaaaaaagt attcaagtat cgataaatag tagagggatg gaggtctacc
     4081 tttcgggaga tttccttaat agttataccg tttctccctt caaaaaccac tatctccagt
     4141 attttaatag cctgttctaa agcaccaata gaacttttag gaggcattat ctattcacct
     4201 tcccggatat agaagaagtc actaaagctt caacaaaatg acggtcaaaa acgggcatat
     4261 ctcctaccac gttcattttt aaagaagcca tggcattgcc ccacagggca gcttctccca
     4321 ggtttttttt cgaaagatat ccgtacagaa atccagccac gaaagcatct cctgctccta
     4381 ttctatccac tgcttcggta gcaaagctag actggtggat agaaacatcc ttactcaata
     4441 caaaacatcc tttttccccc gcagttaaaa catatatttt gtcttctccg taatttgact
     4501 gaaggaaggc taacttttcc caataatctt cattttctaa cccaaaaata acttgcaggt
     4561 cttcttcttt gataaagata atatctatgg acttcattat ttcttccata aattttcgac
     4621 actcctccgg gctccacagt ttgctgcgat agttaacatc aaaagaaatc tgtaaagtgg
     4681 caggcttgaa cttgagtaga ttcctcacgt tcatatcgca agtggggctg aggggaggag
     4741 taataccggt gagatgtaaa agagaactct gggagagcaa ggaaaaatcc tcttctctca
     4801 gctcaaaatg agagaaggta gaatctttcc tatcgtagag aacctctgct ccttctcttc
     4861 gatgagcaaa ctccaggaaa taaagaccca ttcttccttc ttctgtgagc tctaaatggt
     4921 cgacccctat cccaaatctc cttagctctt gaaagactct tcgccccaga aagttgtctg
     4981 gaagagcccc caatagctgg gttttcaatc ccaggatgga acagccggct aagacattag
     5041 cctctgctcc tcctatttcc acttcccagg agtcgctttt cagaaaagtt ttaaatcgct
     5101 caggggttaa gcgaatcaga agctccccat agcttaaaac gtcaactttc ataatctcat
     5161 ccttccaaat attatcttaa atattctctt cctggtactg ttgtgcttta ttttttatag
     5221 tgtacagtat tcaggctaat tctcctactt tccgagcttt caaaatcaaa ctaccaaaaa
     5281 gtaacttcat ttataaattt cttcggttaa ccgatctctt ctccagagtt atagccccat
     5341 cttttaattt ggcgactata tagtgataag cccagctctg gtcatctcgg ggatagagct
     5401 ccatgctatt ctcatcttta aatagtaagt gaggacctct gctttctttt cgctcataca
     5461 tagtctggag aagtaatcga gatagcaaat ggatattcag agtctccaga tagtctttta
     5521 agctttcact agcatggaga atttcttctt cccttatact acaaatttct tcataagccg
     5581 ccttcaaatt atggggaact cttataactc cggcgtttct ggtcataatc tctctaatat
     5641 ggtgccttac ctgattgctc cccccaactt cccgggaaaa ttttggaaac tgccttttta
     5701 ttttttctgt ttcttcttca gtaattccct cttgttttat tgaagaagaa taaattgctg
     5761 cttcttgacc tgatattttt ccgaaaacct gggtgtcaag caaagaattt ccccctggtc
     5821 ggttggctcc atgctgcccg ccggcagctt ctccgcaggc atataatccc cctatagtgg
     5881 tttcggcttt ttcgtttatc tttattcctc cctgaaaatg ctgaaccgca gggactattt
     5941 caattttccc ttcctcgtct aaatttattc ccctgctttt aagtaattca taaatttctc
     6001 tgttcatttt tttaagtctc aataagggtg aggcaaataa atcactcctt aacaaatcct
     6061 catccagtga atttctgtac cactctaata ctttttctgg taaagattct ttttctaaaa
     6121 aattgggatt ctgagcatag tttaaaaaaa cccgtctgcc ttgctgaatc tctttagcta
     6181 cagctacatc gataatgtga gattcttcct cgcaagagag aggccagctc catcccttgg
     6241 aaaaaagaat atttaccaat tctttttgag aatatttatc tttataatat ttgagtaaga
     6301 catcttcccc ttcgctgttc actatttcag gtaaagacct cataaagctc ccggaacagg
     6361 ctatttttgt tttcttagaa gacagaccta tctgaataaa ctccatattt accaattctg
     6421 caccagctct cagcgccgct ccccatccat ctccagtagc cagaggaggg aagagattat
     6481 ccataaacac ctgtcctgca ccaccggtag ccataattat ggctggagca tcgacgaaaa
     6541 aatactcatt atccaggatt tgaagccccc aggcaccctt aaccctgttt tcttcatcca
     6601 ataaaaggcc ttgaatcata gtattttcta taacttctat gttttcttct ttaacttttt
     6661 tagccagtgc tttcgaaatt tgaacagcag tgtcaggccc ggtaaagcaa gcccgggcat
     6721 attttgagcc gtcggtcctg aattgtttaa ctctatttcc ctctcttgca aaagggactc
     6781 ctaaattatc cagatattca aaagcatctt tggagttttt agctaaaact tctgctaaat
     6841 tttggtcaga aaccatacct cctattacaa aaatatcttc tgcatgatac ctccaggaat
     6901 cttcgctgta aggttcagta gtatccagag tggcatggaa tgccattcta tcggagtagg
     6961 aagtggcagt gacgctatct tttaatagct ctcctttagt cactatttta acatctaaac
     7021 ttttattggt ttctttagcg ctcagtgctg ctcttaggcc tgctccacca gagcctacca
     7081 ccagtacatc tgctttcgtt tttttcatcc ttttactcct ggtctctctt tttttctatc
     7141 tcccggtaag gtttcagata aacttcacca tcccggatga cacaaccggt gatttttctg
     7201 tgagccttca gctctgcaca cctgatacag gttatacaaa cttttctttt atccattcct
     7261 ttattctgca gtatatcctt tgcaaaatcg gggtaagcaa aagccattct cccaaaacct
     7321 attatctttg catacccttt ttctaccatt ccagatgcta catatggcgc aaactgtctc
     7381 aaccagctca aacctgttgc caccacgatg atgtctttga aattctcctg gacttcccga
     7441 gataatttta ttagcctctc cactcctaaa aggggatatt caggaggagt atattttctc
     7501 gcctctccat ctgccggcct gctaatgtag gcgttaatat agggacttcc tgctgttaca
     7561 tttattaatt tgacacccat atcccttagc tcacctatca attttaaagg ctcacttaaa
     7621 tctaactctc ctttttcgtt cactccccag ctgatgggat actccagaaa atcggagatg
     7681 ttcaacctta ttgctacttc tattcctgcc tcttttttga ttctcgctac tgcttctttt
     7741 atcagtagag ttctattttc atagctacct ccgaatttgc cctctctttc ccgggcgcca
     7801 aggagctctg acaacaaata tcggtggcaa cttttcacat ctacagcatc aaatccggcc
     7861 tgttttgccc attgagcagc attcacatag ttgtttccta attcttctaa ttctctatca
     7921 gatataacag gatgatgcgg ctccacttta gccaatttgt ctaatttttc gtcgtgaaga
     7981 gcaataattc tgtggtcccc aaaacgcccg gcgtgagata gctgcagaac caggtaagga
     8041 gaatggttac cttcttcatc tgcttcttct ctcattctgt ttatcaacag ggaaaagtcg
     8101 ctcaccgttc tttcattgag aaatagttgc tggtcgttgg ctcgagcttt ttcattaatt
     8161 gcggtagcct caaaccagat aagccccgct tttcctctgg tatatctcat atatcttctg
     8221 gtagtcaatt ctccaggagc accgtcggga gtggaatcgc agccctccat agggtgaatg
     8281 gccagccgat ttcctatggt tttcccgttc agttctatct tttcccggag taaatctaaa
     8341 ttataagaaa aggggatgtc cagattaaat tttttaatgt ccgcaatcaa ctcatccatc
     8401 gtcgaatatt taaagcgttt atggtcattc aagagtatca ccttgaaaat agttgttcaa
     8461 tattttaatc ctcctgccga tatgcctttc ataaatagat tttgaaatag caagaaaata
     8521 accagggtag ggatgatagc cataacagag aaagctaata tttcattcca tggtatacca
     8581 tattgcccct ggagggtagc cagcgctaac tgaatggtga attttttacc gctgttgata
     8641 actatcaaag gaaggataaa gtcattccat ctccaggtaa aagaaaatat ggaaagagag
     8701 gcaattaagg gtttggaaag gggcaatact attcttaaaa aaatctgcca ttctcctgct
     8761 ccatctactt tggcactttc taaaaattcg ttcggtatcc ccctcatata ttgaatagca
     8821 gtgaaagctc cagtaggagt gaaaactgca ggaattatca atccccacaa actatccatc
     8881 atccccagct gccttataat cacaaacaag ggaaccatta ttacctgagc agtaacgaaa
     8941 agtgtaacta tgaccacctc agtgagaaaa cctctatact taaaggtgcc tttagcgagc
     9001 ccataaccta aagagacaca gaggaaaact gttataatgg tagaaattat tgctacaaat
     9061 agggtattag caaggtatct tggaaaatca gattgttcaa taacgtttgc gtaaccttcc
     9121 aaggagattt tttcaggaaa tatggaggga ggatatttat aaatctcgtt catacttttg
     9181 agagaagagg ttatggtcca gaaaacaggt atcatgaaca gtactgccag cacaataatt
     9241 atcacttttt tcatgctgta ccctcccttt ctcctccttc ttgagccttc ctcactgcaa
     9301 aagctatcac aaaagttatc aataacatca ccaatgaacc agcagaggca tatcctatcc
     9361 taaaatctgt aaatgctttc tggtaaacat aaaggggagc aacagtggtg gcatatccgg
     9421 ggccacctgt agtcataata tacactatgg taaaatttct gaagcaatta gctatagata
     9481 acgtgattac agaaatattt gtatttttca ataacggtaa agttatatac cagaattttt
     9541 gaaaacttgt tgctccatct atggaggcag cctcatagaa ttcttctgaa attgcctgta
     9601 atccaacgat aaatatcatc atcataaatc cagctaaaac ccagacttga actacgacca
     9661 cggtaagtaa agctaaagtg gggttatcca gccagctaac tggctggagc cccagttggg
     9721 ctagaatgtg attgaccaga ccaaaatttc tgctgaatag ccacttccac atggttccag
     9781 ctgctaccat gggtatcata tagggccagt aaaagaaaac tctcatgact ttggaaaaag
     9841 tgtcctcttc ctggagcaaa agacctaaaa gcaaagaaat tacaaatatt ataggaacag
     9901 ccagaaaagt atacttaaaa gacactacta cggagttcca gaaccatttg tctttcatta
     9961 aatcggagaa gtttttaagt cccacaaacc tcataggact taagatatcc caggaaaaga
    10021 aacccaggat tatcattaac aaaataggaa ggagataaaa taaaaaatga aaaataaatc
    10081 cgggtatgac aaaagcaaat ctgttttccc ctatcccaca ctttgtcacc agctcacctc
    10141 cccattttta agagttccac aaaggaagag ctgagcctat tttagtaaaa ataggctcag
    10201 ccagcttttt acagctcact taagattctt tgtgcttctt ctttccagtg ctgaatcatt
    10261 tcttgagcag tcatactctc tgaaattcct aaagccagat ctttccttat agtgtcagtt
    10321 aatctactcc atactgcagt agctcttact tcagtactcc aggccggagc tctttctgct
    10381 aagacctggt gaacttctgc ccattcgtct aaaagaggct ctccataatc aatgctgtgg
    10441 tttttatagg cagttaagtc tctgctcctt tttatgtatt ccgtatatcc tttgtctttg
    10501 tctgctatcc acattacaaa gtcggtagca gctttctctt tctccgcatt tccagtttgt
    10561 ggtgcagcta agaaccctcc gcctgaaatt ccgaaccagt cttcaatcac tggaacgtaa
    10621 cagggtgcca cattattttg tttctcagcg ttgaaagatt ctctggccat ccagctacct
    10681 gcccagtcta cagcagttat tccaccgaag aagttgctgg tatgagattc tcccgacagc
    10741 cattcggcag gaggtactaa ttcttcttta aatagaccta caaattcatc tatcgctttt
    10801 tctgcatttt catattggtc cattacaaag tttccttctt catctaatat ctttatgttc
    10861 cacaaagcta aatagctgaa aaatttgtcc gcggaatatt caatagacat ggcatagggg
    10921 gttgcatttt cgcttttcac tttccttaga acgtctttga actcctccca ggtccagggt
    10981 tctgttctac cacctttagg tggataagct attcctgctt tttcgaacat ctctttattg
    11041 atccacacgg agtaagttgt gaagtgaaga ggaacagcgt gaacgatgtc tccttctcct
    11101 aagtagactt ttacaacatc ataggtggag tctttatatt cctggggaga cagtcctaaa
    11161 ttttcttcaa tatcgggacc tacattcctc aaaaattggg tcatttgtgg caaatatgta
    11221 gcagttgttt ccagtaaatc gggtggctct ccacctagaa aaaccagcga catcttctgc
    11281 atatagttgg cataaggaaa tgctaccact tcaattttta cgtccggatg ttcctcctgg
    11341 tataactcta ctacttgtct taggaccctt gctttactct catcagacga atacatggtt
    11401 aactctactg caaaagcaga accaaaagtt aacactacca ataacgctag ggtggtgaat
    11461 aaaagaacct tcttcatgat ttaacctcct ttttaaagta tttactctca tcttttaagg
    11521 ttacctcccg ggggaggcat tacttttaca cttccttacc cggaacttaa gaaatcaaaa
    11581 ctttactcct caccctccgg gtcaaagata tatcactttt gcttcatctg ccgattgcct
    11641 ggctttcagg caaacttctg taatccggaa agaatcttcg gcagttacta aacactttcc
    11701 tctaccttcc acttctttta aaaaatccag gaatatactt ccttctggtt ctaagggaac
    11761 ttctcgaaca cctgctattt tgctatcaat taaaaacact ttatttttaa ttacctcgat
    11821 aattccttca ctacccacca ctcttagctg atcatcatca tgagttggtg cattagcagg
    11881 tctaaaatag tctatagtta aagaggcaaa aacttcattg gtgagtacaa aatggcatag
    11941 agcagtcatc tctaaatctc catggtcctg gttgtctttg gatgaatgag aagcatatac
    12001 tgatacaaat tcttccctgc tgaaccatct tatccaatct actccatgac ttcccaccca
    12061 ggggatagtt cctccgtata cctctctcga tttataattt tctcctcgcc taccaagttt
    12121 gtaagatttt tgagcattta ataaccttac cttccctata gagccttctt ctacataatg
    12181 gtgagcggtt aaaaaccagg gcttatatct aattcccagc atagccacga aggttttttt
    12241 acttctctgg taagcttttt tgaggctttc caaatcttct aatgtggtcg caacgggttt
    12301 ctcagtaaac acgtttatgt ttctcgatag agcttcttta gaaactttag ctaaatctcc
    12361 aaaataacaa tttataaccg ctatatcggg atttatctca tcaaacatgt ttctataatc
    12421 ttcgtaaata agaggtttat atcctaagtt tatgacttcc tggtataagt cttccatgtt
    12481 ttctccttct gatcctggag caattccagc tatttccagg tctaaattac ggttgcactt
    12541 tacagggctc aacacatatt gataatgccc gctcgacccc actatacaca atttcatctc
    12601 aaactatcct ctccctggaa ttatttagag aaaagggatt aaaagcttta taagcttcta
    12661 catttacctc cgtagaagga atggcccact tgatacccaa gtcggaaaaa agagctcttt
    12721 tttcaaaagc ttcatcgatt atctcgtcta taccttccag tactgttttg tattctccgt
    12781 attcagtttc aaattctctc actaattctc ggggaacagg aactatttta ccggccgatt
    12841 cataagcccc atttattgcc actacaaaag gccttgagtt ggctggtgta gaccatgcct
    12901 cttctataag gcctaagtgc ttctgagcag tgaccctaaa aacctctaaa tggggattta
    12961 ctccctgatt atcaaactcc agtacttctc cacggtttaa gaccacttta gttttttctg
    13021 agaattccca gaaagccctg cctttagttc ctgttatttc catgtaaggg tctcttgtcc
    13081 tttcgctggc gtgagtcaca taaaaataca ctttagctcc attcacagcc tcaacttcta
    13141 agcaagaagt atcttctgct tcgatataag tgtgtgctct ataaaattct gctcttacgg
    13201 tcttcagatt aaccgcttcg ttcatagacg gaccaaggag ataaagcata ttatttagat
    13261 agtgggccag agcgttgtgc ataggaccat ctaaaacaat gtgcccatcg taaatagttt
    13321 tccctgccca ctcgtttctg gtgtaataag atttgaacct tggccagcaa gctttgcagc
    13381 ttatttcttt gatttctccc agttctccct gaagaataat ctcttttaac tttctaatag
    13441 ttcgggaatg aatcatgtga aagcctaccg agcaaaacgt tctcgttttt ttctccgttt
    13501 ctataatttt gtccagttcc tggatggtag gagcaggagg cttttccagc aagacattgt
    13561 atccagtttc catagctctg atagccatcg gtgcatgagt atgaatagca gtgggcaaag
    13621 ttataacatc tatcaacttt tttcctttct tcaggagttc agagaaagaa tcatatattt
    13681 ctattccctc tcttttgaac tctttccata actcgaaatt tttcctctta ttttcaacga
    13741 ctatcgctcc taacttgata ttttcttctt tctcgagctg ttggatgaaa tttatatgtt
    13801 ctttagcaaa atttctgact cctaccacgg caaagaatac ttctttcact cgaccacttc
    13861 cctctcttta ctttataatt tcccggctcc tgcctcactg gttactattg ctggcactga
    13921 tattgcagga tcgattttta agaacaaggt aggtttccaa ccaacagatg gttctaattg
    13981 atcatttaca ttaacatcgt tgtgagcttt aaggaggtta gcatagaatg gctggctgga
    14041 aggataggat ataaccgtgc cctcctcata tatacaacta cctttccatc ttcttattat
    14101 tccagaggct ggaactgtcc agggtaagtc aaaatagttg ttttgagcat atattttgga
    14161 ttctactccc acaccccagg catagtcaaa gggataagga gagtgggagt tgattttata
    14221 atagttattg taaacatgaa cttctccaaa tctcacccgg ggaagccttt gagtgacatt
    14281 ttcaaaaaga ttgtgatgga gcgtaaccct taaatggcct ctatcactaa cgttgctatc
    14341 acttgagcct atgagcatcg ttttatcgtg attgtagaaa acattgtatg aaacggttac
    14401 taaatcagat tctttggtga tgtctaagag cccatcatgt tgctgaaaca accttccaaa
    14461 ataagtggga gcttcattat cgagatatct tccatcactg aaagagcagt gatcaatcca
    14521 gatatttttg gagccctcta tagaaacaca atcatattca gaattccatt ctccgaactc
    14581 tccatcagtg ggatcccatt gggggaagta atcaatcggg ctttcaaaag ttaaattgcg
    14641 gattatcaca ttatgagcat ttttaagctt tacggtagct ccaacgattt tagcatcttt
    14701 tcccactcct attatggttt tgttagaacc tatatatatt aaaatatcat cagcctgatt
    14761 cttttgagac cttgctcttg cttcttctaa aggtccagaa acttcctttt cctttcccca
    14821 tttttcagga gcatattctt ccagatattt atcgaagtca aattcaggat ctttataggt
    14881 ttcgtatggc tcacttattt caatggtgcc ctctataaat ataattcttg gaatatcatc
    14941 ttccatgttt ttttctctat ctccgccgag agcttctatc aattcttcct tggttctcac
    15001 taaataaatg ttttctacac tggcttggga gccaccagat gtaccttctc cataagatgc
    15061 ccagccatca cctggcttca gagtttctct acccaaactt agcaattctc tacttatccc
    15121 gggaggcagt tcagtagccc aggaaatacc cgcaatcccc atcaccacta aaaacaccaa
    15181 cactatccta ccgagcggct tcataatatc actcctttgc ataatatgca ttatgattgc
    15241 tctatataca ttatagaaca ccaacttttg gtctgtcaac ct
//
LOCUS       DAOSVE010000034        15011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_2413_length_15011_cov_68.611193, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000034 DAOSVE010000000
VERSION     DAOSVE010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 15011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15011
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2413_length_15011_cov_68.611193"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="PK016_04290"
     CDS             complement(<1..773)
                     /locus_tag="PK016_04290"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011222525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HXL02323.1"
                     /translation="MPANVQDLFNLQGEVAIVTGGAQGLGEQMAAALGESGAQVVVAD
                     LNLEKAQKVASNIQSWGVDSLALRVDVTKKEAVEEMVEKTLERFGKIDILVTSAGVGQ
                     WMKAEEMPEEDWKRVLDINLNGVFLCCQAVGREMIKRKKGSIINISSMSGLVVNTPQA
                     QSHYNSSKAGVIMLTKSLASEWAPYNVRVNTIAPGYMETQLVGDLLKEYPEYAELWKK
                     LTPFGRLGNPQEIKGPCVFLASSASSYVTGSVLVMDGGYT"
     gene            complement(817..894)
                     /locus_tag="PK016_04295"
     tRNA            complement(817..894)
                     /locus_tag="PK016_04295"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(857..859),aa:Pro,seq:ggg)
     gene            complement(937..1275)
                     /locus_tag="PK016_04300"
     CDS             complement(937..1275)
                     /locus_tag="PK016_04300"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HXL02324.1"
                     /translation="MPPFSEEKRFSKLPVTENRWEKFLFIGFLALSLFFFNLHLSNKL
                     VDYFNLAREMKELEKRERTLSEEVEKLEKEREYLDEDWYIEKLARERLQLVKPGEIIV
                     KVINPESPEH"
     gene            complement(1226..2512)
                     /gene="eno"
                     /locus_tag="PK016_04305"
     CDS             complement(1226..2512)
                     /gene="eno"
                     /locus_tag="PK016_04305"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HXL02325.1"
                     /translation="MSTIFDVHAREILDSRGNPTVEVDVTLASGAFGRAAVPSGASTG
                     TFEAVELRDGDKRRYLGKGVRKAVENVNEIIAPEIIGLDALDQAVVDKTLIELDGTPN
                     KGKLGANAILGVSLAVAKAAADYSGLSLYRYFGGANARELPVPLMNILNGGKHADSGV
                     DIQEFMIAPCGARSFSESLRMGAEVFHNLAKVLKRSGYSTGVGDEGGFAPNLHSSEAA
                     IEVILEAIQLAGYEPGKDVYLALDTAASELYKDGIYVFEREGVRRNVEEMIDFYRRLV
                     ENYPIISIEDGLAEEDWEGWKKLTFALGDKVQLVGDDLFVTNKERLLRGIREKCCNSI
                     LIKLNQIGTLTETLETIEIAKRAGYTSVISHRSGETEDSTIADLAVGCNAGQIKTGSL
                     CRSERTAKYNQLLRIEEELDNAAIFRGKEVFKVTSY"
     gene            complement(2509..2910)
                     /locus_tag="PK016_04310"
     CDS             complement(2509..2910)
                     /locus_tag="PK016_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HXL02326.1"
                     /translation="MSKKIEFSFPEKGIAIEAELNDSSLSEEIFRILPLEERVNRWGK
                     EIYFAIPMRSSIVSPQEVVEEGDIAYWPDGACLCIFFGPTPISNGKEIRPASAVEVVG
                     KILSPLDILDEIPEGSIVKITQFDRKGVDRE"
     gene            complement(2895..4046)
                     /locus_tag="PK016_04315"
     CDS             complement(2895..4046)
                     /locus_tag="PK016_04315"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF014634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HXL02327.1"
                     /translation="MRILVAPDSFKGSLKSTEVASFIRKGLQKVDESWQVVERPLADG
                     GEGTVEVLVQGTKGKIIEKEVTGPRGEKIKAHIGVVSGGEVAVLEMAEVAGLILLPPE
                     ERNPRFTTTYGVGELIKEAVSLGFHRIILGVGGSATTDGGMGALRALGVKFFDQEGKE
                     LKGVGDDLLRLVRIDSQHLFTKPEEVELIIACDVKNPLYGEEGAAYVYAPQKGASPEE
                     VEMLDRGLRNYARLVKKHTGIEIDHLPGAGAAGGIGAGLYAFWGARFTSGIQLIMDIL
                     GVEEEIKKADLVISGEGKIDKQSLYGKVVSGVEEKCRKWGKPLLLVGGKISEEAFSLW
                     EEKALALFSIANGPMSEEEAFKKTPFLLEHLSFSLGRVWKGGGVWCLKK"
     gene            complement(4043..4294)
                     /locus_tag="PK016_04320"
     CDS             complement(4043..4294)
                     /locus_tag="PK016_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HXL02328.1"
                     /translation="MRLDKFLKVSSLIKRRPLAQAACKGGRVLVNGKVAKPGMEIKPG
                     DEITLFAPTYKIVVRVKSVGEREKGEELYEMLKKEKIEE"
     gene            complement(4281..4718)
                     /locus_tag="PK016_04325"
     CDS             complement(4281..4718)
                     /locus_tag="PK016_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02329.1"
                     /translation="MGESEREIHAGEKISLAEFLKRLRVILPLVQGWNPDLLIAVNSE
                     GLILSGFLTAFLNKEVRNVALEEKPFQIIWDNLGNLEGKRAVLVAGRFREGKSREELE
                     KWVKEHGALSVLVMVAVGGRGDYSLFPEWGEEVLLPWEDREVR"
     gene            complement(4676..5473)
                     /gene="mazG"
                     /locus_tag="PK016_04330"
     CDS             complement(4676..5473)
                     /gene="mazG"
                     /locus_tag="PK016_04330"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HXL02330.1"
                     /translation="MSSGRAGELFEELIEVVATLRGEGGCPWDREQTRETLKPLMVEE
                     LYEALDAIDKKDEMRLKEELGDMLLHIIFHAQIGKENNTFTIEDILEHLIYKMKRRHP
                     HVFGEVKVKDVDAVLLNWEKIKERERKESGMLSSLPRSLPALLRAKAMQERAARVGFD
                     WKTPEEVEKKVEEEWGEFQEAWESKDTQKMKEEWGDLIFALVNLARHLGVEAEDALQG
                     ANNRFAERFSFIESEIKEQGRELSGVALEEMDALWEKAKEKFTPEKK"
     gene            complement(5451..8531)
                     /gene="mfd"
                     /locus_tag="PK016_04335"
     CDS             complement(5451..8531)
                     /gene="mfd"
                     /locus_tag="PK016_04335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HXL02331.1"
                     /translation="MNSGLGKLVSRIREIEREKLIYLDTISPARPFFALFLEKPLLYV
                     VSNEERKKKVFHQIEALRQIEGKYFSVQEVKREEEFFGVKLQDSFRQEKVIAVVEAQD
                     LPLNVEDLDYFLTHSFLLEEGKETKREEFLDLLFSLGYERQELVREKGEVAIRGEVVD
                     LFPPHLSLPVRTYWWGNRVEKIKFFRPETQRTEEEIKTLFIPPRSGEFKTVPLLSALK
                     KEVKTIFWDGVTVENFTLSSRQAFCGISTSQAEVSFSLEAERIPSFWGKINDLKNYLE
                     ESPWSKIFILLPPEKLEILSSLLEEEKVDFGLHPEEEKKVILSPGYLREGFGIPEAGL
                     AFISSWEIFGKEFAPSRKIPREPLASINSLSPGDYVVHEEEGIGIFRGWKEMTVEGIK
                     RVYVEIGYGGGDVLYLPADKIYLLQKYVGVGEEKPQINRLGKAEWNRTKERAKKAAEK
                     VAQDLVEIYARRQLEKGHSFSPDTPWQKELELSFPFLETPDQKKAIEEVKKDMESSRP
                     MDRLICGDVGYGKTEIAVRAAFKAVMDGKQVAVLAPTTLLSEQHYVTFRERMKDFPVS
                     IAVLNRFRTPQEQKSILEKVEKGEIDIIIGTHRLLQKDVVFRDLGLLVVDEEQRLGVK
                     HKEQLKKLKANLDVLTLTATPIPRTLYLSLLGLRDISVVETPPEGRKPVYTLVVPRDK
                     KVIKEALEREIEREGQVFYVCPRIRDLTPVREELDNLLPGVKIALAHGRMGGKELEEV
                     MENFYRGKISVLLCTTIVGIGLDVPNANTLIVDPATLFGLAQLYQLRGRVGRFDREAF
                     AYFLYPPRLTAEARERLEALMEFSTTGSGMKLALRDLEIRGAGNILGMEQHGFIQEVG
                     FSLYTRLLEEEIARLRGEEEISPINPHIDLKEEAYLPSSYLSKDSERFYFYQKLLGVR
                     KEKEVEEIKEELEDRFGHLPRQAKNLLQITKIRHYAKIAQIESIEEKDGELYFVASLP
                     VLLNLSQYMEEKGKEGKLFNYQGREAFKIKMLPLRDLVSLLEGMVIESVQWQSR"
     gene            complement(8539..9111)
                     /gene="lptC"
                     /locus_tag="PK016_04340"
     CDS             complement(8539..9111)
                     /gene="lptC"
                     /locus_tag="PK016_04340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HXL02332.1"
                     /translation="MAKRLILIIGFCMAGFLLVFFLLSSVSEESGEGEKTEEEIEEPT
                     SSPPVILKGTELRGWEGEKLAWLIQAQNVELSSDNTRAICRGEVKLIVYQEGKEKAII
                     QAQEAEINLDDKDFYLKGEVEVVSSSGDKIVTEELWYRDKDKILETEAEASVYFDENF
                     IHCRGLRSDVDFENPEFYQIIKGSFQLSAL"
     gene            complement(9111..10826)
                     /locus_tag="PK016_04345"
     CDS             complement(9111..10826)
                     /locus_tag="PK016_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXL02333.1"
                     /translation="MNNKVVIIGGVAAGPKVAAKLRRLDPEAEITIIEKGKFLSYAGC
                     GLPYYIGGEIKEYRELMETPIGVMRDPAFFEKTKRIKVLNRTEALRIDRENKRVELKN
                     LESGEISDLPYDYLVLATGAKVSIPPVEAVDLNHPEVSLSPIDLAHVYTLHGVEDAEA
                     IRWTIKEKLAKRAVIIGGGLIGMEMAESLAKSGLEITILEVLPEILPILDEDMGILVR
                     RYCQSEGVNIRVGEGAQRLEGEEGVVKRVVTDKGGYEADMVVIATGFKPNDDLARKAG
                     LEIGERGGIKVDLFLRTSDPFIYAAGDCVEIENLVCGQPAYIPMGSLANKQGRVVAMN
                     LVGRREVFRGALNSVIFKVFDYTVARSGLGVKQAQNLGYDVEYALVPAPDRAHYFPGA
                     KQVIIKLVADRRSGKVLGAQVIGKGEVTKSIYALATLLNYQGKVEDLSNLDLPYAPPY
                     ASAIDNLCLAANVLRNKLEGNMVGVSPQEVERKRKRGEDFILLDVRTPLEYQKVRIPG
                     SVLIPLAELPARIGEFPVDKEIVTICAAGLRGYEAYLILKSRGFQKVMVMDGGMATWP
                     YEVER"
     gene            complement(10863..11162)
                     /locus_tag="PK016_04350"
     CDS             complement(10863..11162)
                     /locus_tag="PK016_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02334.1"
                     /translation="MGTFLKVVGIAFFCLGIVAIIFSLVGMSTQKSSPSYSLPPPGEE
                     FYPFPEEEFFSSTSDFFSKLAGIGGILGGFGVLVGGAALFCLGSIYNDVRSLKNR"
     gene            complement(11173..12240)
                     /locus_tag="PK016_04355"
     CDS             complement(11173..12240)
                     /locus_tag="PK016_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXL02335.1"
                     /translation="MDKFAFIIHAFSSQDIKDHLKFLRILPSSWVEGLASRVSPFVLS
                     EVKGVRSEFTGKEIGGWFIGLPMTSRVLLEYPFSFVAQQIRKCGFLAEKLGAKIIGLG
                     AFTSVAGDGGITVKNGLRIAVTTGDSYTVATALRALEMASSLMEIDLDQEEIAIVGAT
                     GSIGRACALILAEEGKTVRIIGRDPQKVEKVKKEVENIKGEEVKGFTDIEEGIKGSRA
                     LITVTSALGNLLQPAWIERGAVVCDVARPRNVAEEVAKARQDVLVIEGGVVDVPGDPD
                     FGMDFGYPPGKSYACMAETMILTLEGRFEDYTLGKEIQVEKVKEIELLAQKHGFRVSG
                     LRSFGRELSEEEIEEIKKRIL"
     gene            complement(12245..12682)
                     /locus_tag="PK016_04360"
     CDS             complement(12245..12682)
                     /locus_tag="PK016_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXL02336.1"
                     /translation="MAKVSSSLVINGSVEEVYNKAKDVEKLATFLPSIKEVKVLNQEG
                     SVVDSFWKGEFQGREIKWKERDFWDEENKECRFSLIEGDFKQYEGVWRFKEEGEDKTR
                     VELEIEYDLGIPLVGALISKFLKKVMEENAQAMLKALQEVVER"
     gene            complement(12688..13962)
                     /locus_tag="PK016_04365"
     CDS             complement(12688..13962)
                     /locus_tag="PK016_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXL02337.1"
                     /translation="MDDEVKERKKIVEETIDKYVKYVSPGLARLFRFANFSTVEWREE
                     GALVFDIYGEAYIDCIGGFGVFPVGRNHPRVVQKVKEQLDRLALSTRTLFNKQQADLA
                     EMLAQITPGDLQYSFFGNSGAEAVEGAIKLARFYTGRKKLVSAINSFHGKTMGALSVS
                     GREVYKKPFEPLVPETYQVPFDDVEALEKIVDEKTAAVILEPIQGEGGINIPHPHYLS
                     QVKEICDRNGALLILDEVQTGLGRTGKMFACEHYGVAPHIMTLAKGLGGGVLPIGAFI
                     STPEIWKVFEDNPLIHSSTLGGNPLACTAGLETLKILQEENIPAQVAEKGNYWMEELR
                     SLQEQFLDLVKEVRGIGLLIGMEFFDSDVASLLAAEMAERKVLVAYTLNNPQVIRLEP
                     PLTIKKEEIEKVTKALEESLNKVRETLKEIGG"
     gene            complement(14008..14769)
                     /locus_tag="PK016_04370"
     CDS             complement(14008..14769)
                     /locus_tag="PK016_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HXL02338.1"
                     /translation="MDKKAFLIDMDGVLLYGDQAIPGAKEFIERLKEKDYKFLILTNN
                     SRYTPSDLQHRMNNAGIEIDAQHFFSSAMATAQFLSSQKPKGSAFVLGGTGLYQALNE
                     AGYSFTDYRPDFVVLGEMESYPYDKIVRACQLIMQGVPFIATNPDSSGPGEGGIVPAC
                     GAVAALIEEASGYAPYFVGKPNPLILRLALRYMDEHSENAIMIGDRMDTDIKVGLESG
                     LETILVLSGVTKLEDVGKFPYRPNHILGSVADIEV"
BASE COUNT         4189 a         3984 c         2346 g         4492 t
ORIGIN      
        1 gtatagcctc catccataac taaaacgctt cctgtaacat aagaggaagc ggaagaagcc
       61 agaaaaacac agggaccttt aatttcttgt gggttaccta aacgaccaaa gggagtaagt
      121 tttttccaca gttcagcata ttcaggatat tcctttaaga ggtcacccac taactgcgtt
      181 tccatataac caggagcaat ggtgttcact cgaacattat aaggagccca ctcagaagct
      241 aaagatttag taagcatgat tactcctgct ttagaggagt tataatgaga ttgagcctga
      301 ggagtattaa ctaccaatcc cgacatagaa gaaatattta taatactccc cttttttctt
      361 ttaatcattt ctcttcccac tgcctggcaa cacagaaaca ctccatttaa gtttatatct
      421 agaactcttt tccaatcttc ttccggcatt tcttctgctt tcatccattg tcctaccccg
      481 gcagaggtta ccagaatatc aatttttccg aaacgctcca gagttttctc aaccatttcc
      541 tctaccgctt ctttttttgt aacatccacc cggagagcta aggaatccac tccccaagac
      601 tggatattag aggccacttt ttgggctttt tccaaattca aatctgccac tactacctga
      661 gcccctgatt cccctaaagc agcggccatc tgttctccca aaccttgcgc tccaccagta
      721 acaatagcta cttctccttg caggttaaaa aggtcttgga catttgcggg catcttctta
      781 tcctcctttt agtaaaaagg cttttatcgc aagctctggt cggggcgagc cgatttgaac
      841 ggccgaccac tcgcacccca agcgagtgcg cttgccaggc tgcgctacgc cccgactaca
      901 ttcgtttaaa aattatagat acaagaccag agattgtcaa tgttccggtg actcaggatt
      961 aattactttt actataatct cccctggttt caccaattgt aatcgttctc gagctaattt
     1021 ttctatatac caatcttcgt ctaagtattc tctctctttt tctaatttct ccacttcctc
     1081 agacagcgtc ctctctcttt tttccagctc tttcatctcc cgagccagat taaaataatc
     1141 caccaattta ttactcagat gcaagttaaa gaaaaaaaga gacaacgcta aaaaaccgat
     1201 gaacagaaat ttctcccatc tattttcagt aactggtaac tttgaaaacc tcttttcctc
     1261 ggaaaatggc ggcattatcc agctcttcct cgattctcaa taactgatta tacttagcgg
     1321 tcctctctga acggcagaga gaaccagttt ttatttgacc ggcattacat cccaccgcaa
     1381 ggtcagcaat agtactatcc tcggtttctc ctgagcgatg agaaataact gaagtataac
     1441 cagctctttt agctatttca atagtttcta atgtctcggt gagagttcca atttgattta
     1501 acttaatgag aatggaatta caacatttct ctcgaatacc ccggagtaat ctttctttat
     1561 tggttacaaa caggtcatca cctaccagct gcactttatc acccagggca aaggtgagtt
     1621 ttttccatcc ttcccagtct tcctcagcca aaccgtcctc aatagaaatg ataggataat
     1681 tttccaccaa acggcgataa aaatcaatca tttcttctac gttacgacgc actccctctc
     1741 gctcaaaaac atatattcca tctttataaa gctcagaagc agcagtatct aacgctaaat
     1801 agacatcttt acccggttca tagccagcca gctgtatagc ttccagtatt acttcaatag
     1861 ctgcttcgga agaatggaga ttgggagcaa aacccccttc atctcccacg ccggttgagt
     1921 aaccactccg tttcaaaact ttggccaagt tatgaaagac ttccgccccc attctcaagc
     1981 tctcgctaaa agaacgagcc ccacaaggag caatcataaa ttcctggata tctactccac
     2041 tatcagcatg ttttcctccg ttcaagatat tcatgagagg aacaggcaat tctcgagcat
     2101 tagctccccc aaagtaacga taaagagaaa gcccgctata atcagctgct gctttggcca
     2161 ccgctaaaga aactcccaaa atcgcattgg ctcctaactt acccttattg ggtgttccat
     2221 ccagctcaat aagggtttta tccacaaccg cctggtctag cgcatccaaa ccaataattt
     2281 ccggggcaat aatttcgtta acattttcta ccgctttgcg caccccttta cctaagtaac
     2341 gtcttttatc accatcccgc agttccaccg cttcaaaagt accggtagaa gccccggaag
     2401 gaacagccgc ccggccaaaa gctcccgaag ccagagttac atccacctct actgtagggt
     2461 tccctcgaga atcaagaatc tctcgagcat gaacatcaaa aatagtactc attctctatc
     2521 aactcctttt ctatcaaatt gggtaatttt tactatgcta ccttcaggta tttcatccag
     2581 aatatccagg ggagaaagga tttttcccac tacttctact gcactagctg gcctaatctc
     2641 ttttccatta cttatcggag taggaccaaa gaaaatacaa agacaggctc catccggcca
     2701 gtacgctata tcaccctcct ctaccacttc ctgaggagaa acgatagaac tccgcatagg
     2761 aatagcgaaa tagatctcct ttccccaccg attaactcgt tcctcgaggg gtaaaatgcg
     2821 gaaaatctct tccgataggg aactatcatt aagctctgct tctattgcaa ttcccttttc
     2881 aggaaaggaa aactctattt ttttagacac caaactcctc ctcctttcca cactcttcct
     2941 aagctaaaag ataagtgctc taaaagaaaa ggtgtttttt taaaagcttc ttcttcgctc
     3001 ataggaccat tagctataga aaaaagagct aaagctttct cttcccagag agaaaacgct
     3061 tcttcgctga tttttcctcc taccagaaga agaggttttc cccattttct gcacttttct
     3121 tccactccac tcactacttt accataaaga gattgtttat caatctttcc ctctccactt
     3181 atcactaaat ctgctttttt aatttcttcc tctacaccta aaatatccat aatcaactga
     3241 ataccagagg taaatcttgc cccccaaaag gcatataacc ccgcacctat acctcctgct
     3301 gctcctgcac ccggtaaatg gtcaatttct attccagtgt gttttttaac taatcgtgca
     3361 taattacgta agccccggtc gagcatttcc acctcttcag gagatgcacc tttttgtgga
     3421 gcataaacat aagccgcacc ttcttctccg tagagaggat tcttcacatc acaggcgatt
     3481 attagctcca cctcttccgg ctttgtaaac agatgctgag agtcaatacg aaccaaacgc
     3541 agtaagtcat cacccactcc tttcagctct tttccttcct ggtcaaaaaa tttcactccc
     3601 agagcccgaa gagcgcccat ccctccatcg gtggtagcac tgcctcccac tcccaaaatt
     3661 atgcggtgaa accccaagct taccgcctct tttatcagtt ctcccacccc gtaggtagtg
     3721 gtaaaacgag gattcctttc ctcaggtggt aaaagaatta accccgctac ctcggccatt
     3781 tctaaaactg ctacctctcc gccagatact actccaatat gggcttttat tttctctcct
     3841 cgagggccag ttacttcttt ttctattatt ttaccttttg tcccctgcac taaaacttct
     3901 accgtgcctt cccctccatc agctaaaggt cgctccacca cttgccagct ctcatctacc
     3961 ttttgcaatc cctttctaat gaaggaagca acttcagtac ttttcaagct gcccttaaaa
     4021 gaatcaggag caaccagtat cctcactcct ctattttctc cttttttagc atctcgtaca
     4081 attcttcccc tttttccctc tcccccacac tttttactcg taccactatt ttataggtag
     4141 gagcaaaaag tgtgatttcg tctccgggct tgatctccat ccctggctta gctactttcc
     4201 catttactaa aaccctccca cctttacaag ctgcttgagc caggggccgc cttttaatca
     4261 aagaagaaac tttaagaaat ttatctaacc tcacggtctt cccagggtaa gagtacttcc
     4321 tctccccatt caggaaaaag agaataatcc cctcttcctc ccactgctac cataacaagt
     4381 actgaaagcg ctccatgttc tttaacccat ttctccagct cttctctgct ttttccttcc
     4441 cggaatcttc ctgccactag aaccgctctt ttaccttcca gatttcccag attatcccag
     4501 ataatctgga aaggtttttc ttccagagct acatttctaa cttctttatt taaaaaggcg
     4561 gttaaaaagc cacttaaaat taacccttct gaattaaccg ctatcaaaag gtcaggattc
     4621 cacccctgaa ccagaggcaa aataaccctt aaacgtttca aaaattcagc aagacttatt
     4681 ttttctccgg cgtgaatttc tctttcgctt tctcccataa agcatccatc tcctctaaag
     4741 ctacaccaga aagttctcta ccttgctctt taatctcgct ttctataaaa ctaaatctct
     4801 cagcaaaacg attattagct ccctggagag catcctcagc ttcaacaccc agatgacggg
     4861 ctaaattaac cagagcaaaa attaggtctc cccactcttc tttcattttc tgagtatctt
     4921 tgctttccca ggcctcttga aattctcccc actcttcttc tacctttttt tctacctcct
     4981 caggagtctt ccaatcaaaa cctacccgag cggctcgctc ttgcatagct ttagctcgca
     5041 aaagagcagg aaggctacga ggtaaagagg agagcatgcc actctccttt ctttccctct
     5101 cttttatttt ttcccagttt aaaagcactg catctacatc ttttacttta acctctccaa
     5161 aaacatgagg atgacgcctt ttcatcttat aaataagatg ttctaaaata tcttcaatgg
     5221 taaaggtatt attttccttt cctatctgag catgaaaaat gatatgcagt agcatatctc
     5281 ctaattcttc cttcaatctc atctcatctt ttttatctat agcatctaga gcttcataga
     5341 gctcttctac catgagaggt tttagtgtct cccgagtttg ttccctatcc cagggacaac
     5401 ctccttctcc tcgcaaagta gccactactt ctattaattc ctcaaaaagc tcaccggctc
     5461 tgccactgga cactctcgat aaccatccct tctaaaagcg aaactaaatc tcggagaggc
     5521 agcattttaa ttttgaatgc ctctctacct tgataattaa atagctttcc ttctttccct
     5581 ttctcttcca tatactggct caaattcaga agaactggaa gagaagccac aaaataaagt
     5641 tctccatctt tttcttctat actttcaatc tgagctattt tagcataatg ccttattttt
     5701 gtaatctgta aaagattctt agcctgccgg ggaagatgtc caaaacggtc ttccagttct
     5761 tcctttattt cctctacttc cttctctttt ctcactccca gtagcttctg atagaaataa
     5821 aatctttcac tatctttaga aagataagac gagggtaaat aagcttcttc ttttaaatct
     5881 atatgagggt taataggaga gatctcctct tctccccgca ggcgagcaat ttcttcttct
     5941 aaaagacgag tataaagaga aaaacctact tcctggataa aaccatgctg ctccattccc
     6001 aggatattac ccgctccccg aatttccaga tcccggagag ccagtttcat acctgaacca
     6061 gtggtgctga actccataag agcttctaat ctctcccggg cttctgcagt taagcgagga
     6121 ggatataaaa aataggcaaa agcttctcta tcgaatctcc ccactcttcc ccgcaactga
     6181 tatagctgag ctaacccgaa caaagtggcc gggtctacga taagagtatt agcattgggg
     6241 acatctaagc caatccccac aatagtggta cagagtaaaa cggaaatttt accccgataa
     6301 aagttttcca taacttcttc taattcttta cctcccattc ttccatgagc taaagctatt
     6361 tttaccccgg gaagcaaatt gtccagctct tccctcaccg gcgtcaaatc ccgaatccgg
     6421 ggacaaacat aaaaaacctg gccttctcgt tctatctctc tttctaaagc ttctttaatt
     6481 acttttttat ctcgaggtac aaccagagta taaactggct ttctcccttc cggtggcgtt
     6541 tctaccacac tgatatcccg aagacctaaa agagaaagat atagagtacg aggaataggg
     6601 gtagcagtaa gagttaaaac atctaagtta gctttgagtt ttttcaactg ctccttgtgc
     6661 tttactccca aacgctgttc ttcatccaca accaataaac ccaaatctcg aaaaaccaca
     6721 tctttttgta gaaggcggtg agtaccgatt ataatatcta tctccccttt ttctactttt
     6781 tccagtatgg acttctgctc ctgaggagta cgaaaacggt ttaaaacggc gatactcacc
     6841 ggaaaatctt tcatcctttc ccgaaaggta acgtagtgtt gctcagaaag aagagtagta
     6901 ggcgctaaaa ccgctacctg ttttccatcc atcactgcct taaaagccgc ccgcacagca
     6961 atctctgttt ttccatatcc cacatctcca caaatcaagc gatccatagg gcgagagctt
     7021 tccatatctt tttttacttc ttcgatagct tttttctggt ccggggtctc cagaaaagga
     7081 aaggataatt ctaattcttt ttgccaggga gtatcaggag aaaaactgtg tcccttttct
     7141 aactgacgtc gagcataaat ttctaccaag tcttgagcta ctttttctgc cgctttttta
     7201 gctcgttctt tagttctatt ccactcagct tttcccaggc gattaatctg gggtttttct
     7261 tcacccaccc ccacatattt ctgcaggaga tagattttat ctgcgggaag ataaagaaca
     7321 tctcctccac cataacctat ctccacgtac actcgtttta tcccctctac agtcatctct
     7381 ttccatcccc gaaagatacc aatgccctct tcctcatgca ccacataatc cccaggagaa
     7441 agagagttaa tagaagcaag aggctctcta ggaatcttcc gggaaggagc aaattccttt
     7501 ccaaaaatct cccaggaaga gatgaaagct aaaccagctt caggtattcc aaaaccttcc
     7561 ctcagatatc ccggagagag aatgaccttt ttttcttctt cgggatgtaa cccaaagtct
     7621 actttttcct cttctaaaag agaagaaaga atctccagct tttctggagg gagaagaata
     7681 aaaattttac tccagggact ttcttccaga taatttttta aatcattaat tttcccccaa
     7741 aaagaaggta ttcgttctgc ctcaagagaa aaagaaacct cagcctggga agtgctgatt
     7801 ccgcaaaagg cctgccgaga agaaagggta aaattttcca ctgttacccc atcccagaaa
     7861 atggttttta cttccttctt taaagcagaa aggagaggta cagtcttaaa ctctccactc
     7921 cgaggaggaa taaaaagagt ttttatttct tcttcagtgc gctgggtttc agggcgaaaa
     7981 aattttatct tttccacccg gtttccccac cagtaagtgc gaaccggaag agaaagatga
     8041 ggaggaaaga ggtctactac ttcccctctt atcgctactt ctcctttttc tctaactaat
     8101 tcctgacgct catagcctaa actaaaaagc aggtctaaaa actcctccct tttagtttct
     8161 ttcccttcct ctaaaagaaa agaatgagta agaaaataat ccaggtcttc cacattcaag
     8221 gggaggtctt gagcttctac taccgctata actttttcct gcctgaagct atcctgtaac
     8281 tttactccaa agaactcttc ttctctttta acctcctgca ccgagaaata tttaccctct
     8341 atctggcgta aagcctctat ctggtgaaaa actttctttt ttctctcttc attactcacc
     8401 acataaagga gaggtttttc cagaaaaagg gcaaaaaaag gccgggccgg ggaaatggta
     8461 tccaggtata ttaacttttc tctctctatt tcccttatcc gggaaaccaa cttacctaaa
     8521 ccagaattca taaacctctc ataaagcgct cagttgaaaa cttcccttta taatctgata
     8581 aaattcggga ttttcaaaat cgacatcgct acgtaaaccc cgacagtgga taaaattttc
     8641 atcaaaataa accgaagctt ccgcttctgt ttccaggatt ttatctttat cccgatacca
     8701 caattcttcg gtcactattt tatcccccga agaagatact acctctactt ctccctttaa
     8761 ataaaaatct ttatcatcca gatttatttc cgcttcctgt gcctggatga tagctttctc
     8821 ttttccctcc tggtaaacaa tgagttttac ttctcctcgg cagatggctc tcgtgttatc
     8881 actactcaac tccacgtttt gtgcctgaat taaccaggcc aacttttctc cttcccatcc
     8941 ccgaagttct gttcctttta gtataactgg aggtgaggaa gttggttctt ctatttcttc
     9001 ttctgttttt tctccttctc cggattcttc agaaacagaa gagagaagga aaaagaccag
     9061 tagaaaacct gccatacaaa agccaataat tagtattaac ctttttgcca ttaccgctct
     9121 acttcataag gccaggtagc cattccccca tccattacca taactttctg aaaacccctg
     9181 cttttcaaaa ttaaataggc ttcataaccc cgtaaacctg cagcacaaat agttacaatt
     9241 tctttatcaa cagggaactc gccaatcctc gctggtagtt cagcaagtgg gataagaacg
     9301 ctacccggta tgcgaacttt ctggtattcc agaggagtgc gcacatctaa caaaataaag
     9361 tcttctcctc gttttctctt tctttctact tcttgagggg aaacacccac catatttcct
     9421 tccagtttat tacgcaggac attagccgcc agacataaat tatctatagc agaagcataa
     9481 ggaggagcat aaggcaaatc cagattggat aggtcttcca ctttcccttg gtaattcaga
     9541 agagtagcta aagcgtagat acttttcgta acctcgcctt taccgatcac ctgtgcaccc
     9601 agaactttgc cacttcttct atccgctacc agtttaataa tcacctgctt tgctccagga
     9661 aagtaatgag ctcggtccgg agcaggtact aaagcatact ctacatcata gcctaaattc
     9721 tgcgcctgct tcactcccag gccacttcga gctacagtat aatcaaaaac tttaaaaatt
     9781 accgagttta aagctcctcg gaaaacttcc ctgcggccta ctaaattcat agctaccact
     9841 cttccctgct tattagccag agaccccatg ggtatataag caggttgacc gcaaaccaga
     9901 ttttctattt ctacacaatc tccagcagca taaataaagg ggtcactagt gcgcaaaaat
     9961 aggtcaactt taatccctcc tcgttcccct atctctaagc cagctttgcg agctaagtca
    10021 tcattgggtt tgaagccggt agctatcact accatatctg cttcataacc tcccttatca
    10081 gtaactactc tttttactac cccttcttct ccctccaggc gctgtgctcc ttctcctacc
    10141 cggatattta ctccctcact ctggcagtaa cgacgcacca aaatccccat atcttcatcc
    10201 agaatgggta aaatttccgg taacacttcc aggatggtaa tctctaaacc actttttgcc
    10261 aggctttcag ccatctccat tccaatcaat cccccgccaa taattactgc tcgcttagcc
    10321 agtttttctt taatggtcca gcgaatagct tcagcatctt caacaccgtg taaggtatag
    10381 acatgagcta agtcaatggg ggaaagagat acctcaggat gattcaaatc tacagcctca
    10441 acagggggga tagagacctt agcaccagta gctaaaacta agtaatcata aggcaagtcc
    10501 gaaatttcac ctgattctaa attctttagc tctactcttt tattttccct gtctattcga
    10561 agagcttcag tgcggtttag tactttaatt cttttggtct tctcaaaaaa tgccgggtct
    10621 cgcattactc caataggtgt ttccatcagc tctcgatatt cctttatctc tcctcctata
    10681 taataaggta gaccacaacc agcataagag agaaatttac ctttttctat aatggtaatt
    10741 tctgcctcag ggtccagacg gcgcaacttc gccgccactt taggaccagc cgctactcct
    10801 cctataatta ccactttatt attcacctgt cacctccggt ttctcaatgc atttcttggc
    10861 cctcaccggt tttttaagct tcttacatcg ttatagatac tacccaggca aaaaagagct
    10921 gctcctccca ccaacactcc aaatcctcct aatattcctc ctatacccgc taacttagaa
    10981 aaaaaatcag aggtggaaga aaaaaactct tcttctggaa aaggatagaa ctcttccccc
    11041 ggaggaggaa gagaataaga aggagaactc ttttgcgtac tcatccctac cagagaaaaa
    11101 attatcgcca ctattcccag acaaaaaaac gcaatgccta ctaccttcaa aaaagtgccc
    11161 actatatccc tcctataaaa ttctcttttt tatttcctct atttcttctt cacttaattc
    11221 tcttccaaaa ctccgcaaac ccgaaactct aaatccatgt ttttgagcca gtaactcaat
    11281 ctctttcact ttctccacct gtatttcttt tcccagagta taatcttcaa accgcccctc
    11341 cagagttaaa atcatagttt cagccatgca agcataagat ttccccggag gatagccaaa
    11401 atccatgcca aaatcaggat ctcccggaac gtcaaccact cctccttcga taactaaaac
    11461 atcttgtctt gcctttgcca cttcttctgc tacattgcga gggcgagcca catcgcagac
    11521 caccgctcct cgctcaatcc aagccggctg gagcaaattt cccagagctg aagttaccgt
    11581 aataagcgct cgagagcctt ttatcccttc ttctatatcg gtaaaacctt tgacttcttc
    11641 tccttttata ttttctactt ctttttttac tttctctact ttctgggggt cccggccaat
    11701 tatccttact gtttttccct cttcagcaag tatcaaagca caagctcgac caatagagcc
    11761 ggtagctcct actatggcaa tctcttcctg gtctaagtcg atctccatta gagaagaagc
    11821 catttccaga gcccggagag cggtagccac agtatagcta tcaccggtcg ttaccgcaat
    11881 acgcaaacca tttttgactg taatccctcc atctccagcc actgaggtaa aagctcccag
    11941 accgataatt ttcgccccta acttttcagc cagaaatcca cacttgcgaa tctgttgagc
    12001 tacaaaagaa aaaggatatt ccagaagcac ccgggaagtc atgggaagcc cgataaacca
    12061 tcctcctatc tctttacctg taaactctga cctcactcct ttaacttctg atagaacaaa
    12121 gggagaaact cgagaagcta agccttccac ccaggaagaa ggaagaattc ttaagaactt
    12181 aaggtggtct tttatatcct gggaactgaa ggcatgaatg atgaaagcaa acttgtccat
    12241 ataatcacct ttctactact tcttgcaaag cttttaacat agcttgagca ttttcctcca
    12301 taactttttt caaaaactta ctgattaaag ctcccaccag tggtatcccc aggtcatatt
    12361 caatttctaa ttctacccgg gttttatcct ctccttcttc cttaaacctc cataccccct
    12421 cgtattgctt aaaatctcct tctataaggg aaaagcggca ttccttattt tcctcatccc
    12481 agaaatctct ttctttccac ttaatctccc ttccctgaaa ttcccccttc caaaaactat
    12541 ccactaccga accctcttga tttaagactt ttacctcttt tatactaggt aaaaaagtag
    12601 ctagcttttc tacatcttta gctttgttat aaacctcttc cacgctcccg tttatcacca
    12661 aagaagaaga aactttagcc attttaccta accccctatt tcctttaatg tttctctaac
    12721 tttattcaaa gattcctcaa gtgccttagt aactttctct atctcctctt tttttatggt
    12781 aagaggaggc tctaaacgaa ttacttgagg gttattgaga gtataagcaa ccaaaacctt
    12841 tctttctgcc atttccgctg ccaaaaggga agccacatcg gaatcaaaaa attccattcc
    12901 tattaaaagg cctatccctc gcacctcttt tactaaatca agaaactgct cctgtaacga
    12961 ccggagctct tccatccagt aattaccttt ttccgctact tgagcaggaa tattttcttc
    13021 ttgtaggatt tttaaagttt ccagccccgc agtacaagcg agaggattac cccccaaagt
    13081 agagctgtga atgagagggt tatcttcaaa aactttccag atttccggag tacttataaa
    13141 cgcccctata ggcagcactc ctccccctaa gcccttagct aaagtcataa tgtgaggagc
    13201 taccccatag tgttcacagg caaacatttt ccctgttcta ccaagaccag tttgtacttc
    13261 atccagaatc aggagcgctc cattacggtc acaaatctcc tttacctggg aaagataatg
    13321 gggatgggga atgttgattc ctccctctcc ctgaataggc tctaaaatta ctgccgctgt
    13381 tttctcgtct acgatttttt ccagagcttc tacatcatca aaaggcactt ggtaagtttc
    13441 tggcaccaga ggctcaaaag gttttttata cacttctctt cctgaaacac tcaacgctcc
    13501 catggttttc ccatggaaac tattaattgc cgaaaccaac ttttttcttc cagtataaaa
    13561 gcgagctaat tttatcgctc cctctaccgc ctctgctcca ctgttaccaa agaaggaata
    13621 ttgcaagtct ccaggggtaa tctgggccag catctccgcc aaatcagcct gctgcttatt
    13681 aaaaagagtg cgagtagaaa gcgctaacct atctaattgt tctttcactt tttgcactac
    13741 tcgaggatgg tttcttccca ccggaaaaac cccgaaacct ccaatacaat cgatataggc
    13801 ttccccatag atgtcaaaaa ccagagcccc ttcttctctc cactccacag tggaaaaatt
    13861 ggcaaaccgg aacagacgag ccaaccccgg actcacatat tttacatatt tatctatagt
    13921 ttcctccact atttttttcc tttcttttac ttcatcatcc ataatctttc ctcctttatt
    13981 tattattttc ttactttttc tctactttca aacttctata tccgctaccg agccaagaat
    14041 atgattggga cgataaggaa atttacctac atcttcaagc ttagtaaccc cagagagaac
    14101 cagaattgtt tctaatcctg attccaatcc cactttgata tcggtatcca tccggtctcc
    14161 tatcattatg gcattttctg aatgttcatc catatacctt aaagccagac gtaagatcaa
    14221 aggattgggt ttacccacaa aataaggagc gtatcccgaa gcctcttcta tcaaggccgc
    14281 cactgcccca caagcaggaa caatccctcc ttcccccggg ccggaggaat caggattagt
    14341 agcaataaaa ggtactccct gcataatcaa ctgacaggct cgcacgattt tatcataagg
    14401 ataagactcc atctctccca agacaacaaa atcaggtcta taatcagtaa aggaataacc
    14461 tgcttcattg agagcctgat acaaccctgt cccccctaaa acaaaagcag agcctttagg
    14521 tttttgagaa cttaaaaatt gagcagtagc catagcactg ctaaaaaaat gctgagcatc
    14581 tatttctata ccggcattat tcatccgatg ttgaaggtca gaaggggtgt aacgagagtt
    14641 attagtcagg atcaagaact tatagtcttt ctcttttaag cgttcaataa actccttggc
    14701 tccaggtata gcctggtcgc catagagcaa aactccatcc atatcaataa gaaaggcttt
    14761 tttatccata atctcacctc aaaaatcaca tctattataa tttctccccc tcctattagc
    14821 aaataaatat ttaataggga tagtgtcaac tcgttgtagg agtattttta tttttcctat
    14881 gttattagct tttgagattc ctgctatcca ttatagtatc ctgcaaaact ggttaaaaac
    14941 tttaggaagt aaattgtatc gaaagtcaaa aactcaaggt cgctacgcat agagaaagac
    15001 tttgtctttc t
//
LOCUS       DAOSVE010000035        14850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_2440_length_14850_cov_69.910916, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000035 DAOSVE010000000
VERSION     DAOSVE010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 14850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14850
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2440_length_14850_cov_69.910916"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..261)
                     /locus_tag="PK016_04375"
     CDS             complement(73..261)
                     /locus_tag="PK016_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02339.1"
                     /translation="MKKSEPASEKVKIKEAEDPANWYMMHELLSLPLSFRLHPYLRKM
                     NLYYFQASDRLIVNRKSS"
     gene            complement(248..1231)
                     /gene="floA"
                     /locus_tag="PK016_04380"
     CDS             complement(248..1231)
                     /gene="floA"
                     /locus_tag="PK016_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052352.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HXL02340.1"
                     /translation="MVFLNYLVPLIIVVVAFLVFLYLVPLGLWISALAAGVKVSITTL
                     IGMRLRRVEPASIILPLIKATKAGLGLDVGLLEAHYLAGGDVDRVVNSLIAAERAGIP
                     LSFERSCAIDLAGRNVLEAVQLSVLPRVIETPEVTAVAKDGIELKVKARVTVRANIDR
                     LVGGATEATIIARVGEGIVTTIGSSRSHKEVLENPDSISKTVLEKGLDAGTAFEILSI
                     DIADIDVGQNIGARLQGDQAEADKRVAQAKAEEKRAMAVAREQEMKAYTQEMRAKVVE
                     AEAEIPKAIAQALREGKLGVFDYYSLQNLQADTQMRKSFGEKGERREDEKI"
     gene            complement(1234..2592)
                     /locus_tag="PK016_04385"
     CDS             complement(1234..2592)
                     /locus_tag="PK016_04385"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HXL02341.1"
                     /translation="MLKAPKYWLVGIIIFLFFLMGSSTLAQSEEKVIFWVPVKDVPNL
                     GMVDWGLASFVSRAFREAEEAGAQTVVLEIDTSGGLVDAMLFIKDAIISSPCYTVAFI
                     NHRAWSAGTFIALACETIVFAPDATMGAAEPRSGAAGEVEQPDPKTVSAIRAQIEALA
                     EARGRDPLLFASMVDRNLEIDGLVKKGELLTLRASQALEHGAGDLVAATREELLNKLG
                     LEGTVRELSPSWSENLARLITNPVIVPVLLVLALGGILLEVLTPGFGIPGIIGLVSFS
                     LFFGGRYLAGLAGWEPLIFFLIGAVLLVVELFLPGFGITGISGIGVLALSIYLVLRET
                     SILFWQEALYQLFFYIALMGGVFLVLLAFLPQSPVWQRLGLRKEITGRAYETEEKGWE
                     PSLVGKTGIAKSMLRPAGIVEIEGKRWDAITEGEFISPGTRVKVEKIKGNTIVVKSEE
                     GG"
     gene            complement(3131..4087)
                     /locus_tag="PK016_04390"
     CDS             complement(3131..4087)
                     /locus_tag="PK016_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010270795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HXL02342.1"
                     /translation="MKLPERASQSTMYFIGVTTTQSSIMKLFPLWAKELGLNNAVLKG
                     IDIEIHAPREVYREAVNFIKTDPLSLGALVTTHKIDLYEAAKDLFDYLDPYAQLFGEL
                     SSISKRDGRLEGHAKDPISCGLAMEAFIPPNFWKEYGGEVFIMGAGGSARAIAAYLFD
                     PKKEGNMPSKLYVSNRSTPRLYGMEEIMKKINPHINAEYIHSPQAEINDQILKRIKPY
                     SLVVNATGLGKDRPGSPLTDNCEFPPHSLVWELNYRGDLKFIHQALAQREEKDLKIED
                     GWIYFLHGWTQVVAQVFHIEIGKELFQRIEEISRDFRPPRLY"
     gene            complement(4089..4652)
                     /locus_tag="PK016_04395"
     CDS             complement(4089..4652)
                     /locus_tag="PK016_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013466279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HXL02343.1"
                     /translation="MKPFNVVIDFSTGELSPGKKTIRRVSDVKNMFLDREKAEEMIKN
                     GDPLVYEVIYVDIPEEAGHLGNCTTIIYPGKVGKEFFLTKGHLHEQLGTAEIYFCLRG
                     KGKLLMESTDGEWAALDMFPGSASYIPPFWSHRTVNVGQEPLIFYCIFPADAGHDYAT
                     IEEKGFPKLVVEENGEVVLIDNPKRSK"
     gene            complement(4683..5708)
                     /locus_tag="PK016_04400"
     CDS             complement(4683..5708)
                     /locus_tag="PK016_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HXL02344.1"
                     /translation="MKAAVFSYPPKVQVVEVPRPRVGEGEVLIRVKAVALCGTDLRIA
                     RRGHADIPEGEKRVLGHEVVGVIEEVGKGVEGLTPGMKVAVAPNYGCGKCYYCIQGLT
                     HHCPEGKALGITENGGFAEFMVVPQKAVFQGNIFPLPEETDLVSIAFVEALACVVHGF
                     LPLRVNFRDRVLIYGAGPIGLMFLELARLRGAKELWMADLSEDRLKIAQERGVKTIVV
                     GKEKVKEKIKEADVVVVAAPSPDAQKEALEVAGVYGRINFFGGLPPQIKEVALPSNLI
                     HYKELTVTGTTKSNNFHFRSALDLLLQKQLDLSYLVSHVLPLERIEEGLSLMEKQQSL
                     KVVIVPE"
     gene            complement(5735..7261)
                     /locus_tag="PK016_04405"
     CDS             complement(5735..7261)
                     /locus_tag="PK016_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HXL02345.1"
                     /translation="MPYLIGSDLGTTATKTGLFDEEGNLIALARQKSNLIYGKDGSVI
                     QDPYEMLNSVVETVGEVVEKSGVSPSSILAIALDGQMAGIMGVDESGEAATPYDSWLD
                     VRCAPYTELMRDKAEELIIKRSGMAPSINHGPKILWWKKEQPEVYQKIDKFTVPSCWV
                     SQKLSGLRGEETFIDYTYLHFSCFSDLENNCWDRELMEIFGMEESKFPLIVKPWQVVG
                     KVKKEWAEKMGILVGTPVVAGCGDQAANNLGAGVVEKGTAFDVAGTASCFSLSVDKFV
                     PDLEHKTILFPRAVLDGYYYPMAYINGGGLDLEWAKDEFFREWANLPHAFQLIDDEVE
                     KIASSPSSLVFLPHLRGRNCPGEPYFRGVFAGFSWDHRREHLFRAILEGVAFEYAYYL
                     SIIRKLLPEVNFREVRVIGGGAKSSFWNKIKASILHIPYVELDREEFAIWGAALLAGF
                     GVGLFSDLKEKSIHSVQKVKTYFPQEEWSSFYNNLFPFYLDTLEKMGKVFDSHKELFS
                     "
     gene            complement(7264..8289)
                     /locus_tag="PK016_04410"
     CDS             complement(7264..8289)
                     /locus_tag="PK016_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXL02346.1"
                     /translation="MDKLRFVLVGAGRAGMVHAQNLVHYIPEGELKAVVDVDLKRAEE
                     ASKNLGVKSFYSTLDEALQKEDFDAVCIASLTFTHREMIEKAAKAKKHVFCEKPLAQN
                     SEEVQKIQEAVEKAGIKFQIGFMRRYDPGIKKAWEMVREGKIGDVVLIKSTGRGPGLP
                     APWIFDRAKSGGMLAEVSAHDIDSVLWFAGQKPHHLSLEAKNFKGKDIKEKFPDFYDH
                     YLLTLNFTDGPLGVVDGGCPVGYAYDARMEILGTEGMIKVGEVEAGGPVLYTPDKKSW
                     CDNQSSWRDRFREGYLEELKSFIRSVLENQNPSPSIIDGKRVVKVVELAHKSLSQQSP
                     VPWEGEW"
     gene            complement(8326..9069)
                     /locus_tag="PK016_04415"
     CDS             complement(8326..9069)
                     /locus_tag="PK016_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXL02347.1"
                     /translation="MEQKLNRKIPVPLYYQLKEIIKEEIEEGKLNPGDSIISERELSE
                     KYGISRPTVRQALKELVYEGWLTREKGKGTFVSKPKIDYGFIQHFTTFYDDMIEKGYS
                     LHTIILNREIRKAGKEIARFLQIGEDEKIIFIDRLRFIEDEPIVRVNNHIPLVLCPGL
                     MEEDLTDQSLYRIMAEKYGLTPYRAEISLEPIVANEVDSELLGIEKGAPMFLMENVTF
                     TEEGQIIDFFRSRFRGDKGKVRVNVYKER"
     gene            9534..9740
                     /gene="rpmE"
                     /locus_tag="PK016_04420"
     CDS             9534..9740
                     /gene="rpmE"
                     /locus_tag="PK016_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HXL02348.1"
                     /translation="MKKEIHPEYKETRVICACGNTFVTRSTKFPEIKVEICSKCHPFF
                     TGQQKLVDTAGRVEKFRNKYGDNY"
     gene            9791..10453
                     /gene="thyX"
                     /locus_tag="PK016_04425"
     CDS             9791..10453
                     /gene="thyX"
                     /locus_tag="PK016_04425"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HXL02349.1"
                     /translation="MKVTLLNYTPDPEITVALAARTCYSSKPPLTISKERAEHLIREL
                     IERGHESVLEHASFTFFIEGISRVTSHQLVRHRLASYSQQSQRYVSMKEANFIIPPSI
                     AENPEAQGLFLKAIEEDKKTYQELVSLGIPQEDARYMMAQGVATNLVLSANARELLHI
                     FNLRLCNRAQWEIRELAQKMLELVKDLAPAIFEWAGPPCVEGECREGENSCGKPWKKR
                     EK"
     gene            10497..11348
                     /locus_tag="PK016_04430"
     CDS             10497..11348
                     /locus_tag="PK016_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HXL02350.1"
                     /translation="MMRSPFRLAMAVRKPNGDIALEVKEQKPITKRYPFLSWPVIRGI
                     VSLIDSLVVGIKALSYSASLALDEEEEKLSSMDIGISILLALGIFVGLFVALPTFLSS
                     LVDQFISSTFVYNLVEGLIRIGIFLAYLLLISQMKDIKRVFEYHGAEHKAIFTWENGE
                     ELTPENARKFTTLHPRCGTNWLIIVLIISIFAFSFLGRPGLTMRVVSRILLIPLVAGL
                     AYEAIKFLSRYQDNHLAYILTLPGLLLQRITTREPDDSQLEVAFTALKGSLREDNYVA
                     GENRGNS"
     gene            11317..12387
                     /gene="prfA"
                     /locus_tag="PK016_04435"
     CDS             11317..12387
                     /gene="prfA"
                     /locus_tag="PK016_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012818219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HXL02351.1"
                     /translation="MWREKIEEIVKEYQDLTQKIANPQLMKDMATYRNLTQTLVEIEP
                     VVKKYEEYEKKERELEENRELLHTESDPELRILLEEEIEKLKTSLPELEEELKTMLIP
                     RDPRDEKNTLVEIRAGTGGEEAALFVADLFRMYSRYAERNGWKIEIMSSNPTELGGFK
                     EIIFAVEGKGAYSKFKFESGVHRVQRVPVTEAGGRIHTSTATVAVLPEADEVDVEIKP
                     EDIRVDVYRSSGPGGQSVNTTDSAVRVTHIPTGMVVTCQDEKSQHKNKAKALRILRSR
                     LLEMERENQKEEIDQERRTQIGSGERSERIRTYNFPQNRVTDHRINLTLYRLESVLEG
                     ELDEIIEALAAEEKMRTLEKVS"
     gene            12388..13269
                     /gene="prmC"
                     /locus_tag="PK016_04440"
     CDS             12388..13269
                     /gene="prmC"
                     /locus_tag="PK016_04440"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HXL02352.1"
                     /translation="MPSVKEVWQKLVKDLKEHSFLSPGREARLLLASVLGVEPSRVYL
                     FWDTELSGEKLTRLHSILEKRRQGVPIQYLAGEWEFFSLPFRLKRGVFIPRLDTESWV
                     EEAIVYLRSLPEEKIIICDMGCGSGVIGISCAFCIENLYLYGADISPQAVKLSEKNAH
                     LLGVDKRSAFLLSDLFSAFPPLKFDVILSNPPYIREKDWDSLPPEVRLYEPREALLAG
                     EEGLDIIGRILEEGEPFLKEEGLFFLEHDPEQKEAIQKLADALSLEYLRSIPDLTGKE
                     RASVIRKRRSKNESTPR"
     gene            13250..13696
                     /gene="rpiB"
                     /locus_tag="PK016_04445"
     CDS             13250..13696
                     /gene="rpiB"
                     /locus_tag="PK016_04445"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HXL02353.1"
                     /translation="MKVLLGSDHGGFELKEDLKVFLEELGHEVEDYGTYNTQPCDYPD
                     VAFQVAEDIIKGKGERGILICGTGIGMSIAANKVKGIRASLCHDTFSARFAREHNDAN
                     ILTLGGRVLGKGLAREIVRVWLEANFTGERHARRVNKIEEYEKNER"
     gene            13677..14150
                     /locus_tag="PK016_04450"
     CDS             13677..14150
                     /locus_tag="PK016_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HXL02354.1"
                     /translation="MKRMRDRPDWDTYFMSIAHLVSTRSTCLRRQVGAVMVRDKQILT
                     TGYNGVPQGITHCTSETCLRSLKNIPSGQQQELCRGLHAEQNAIIQAALHGVSTKEAT
                     LYCTHKPCILCAKMLINAGVARIVYQNFYPDPLADQMLEEAGIEMCIWEGQVISY"
     gene            14147..>14850
                     /locus_tag="PK016_04455"
     CDS             14147..>14850
                     /locus_tag="PK016_04455"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HXL02355.1"
                     /translation="MKQVLFFSFLSCILALAIVPLVIRFSKKKKWFDIPDGKRKIHTK
                     PVPRLGGIALFLAFFLAVFIISLRGSFPFPFFLLAGGGVIFLTGLIDDLLSLPPWGKL
                     IGQIAGALILIAGGVTIQFFTLPWDKMVYLGSWGYPLTLLWIVGITNALNLIDGMDGL
                     SAGIGAIASFTLGVIAWQEGRIEPAALSFLLAGATIGFLFYNFPPAKIFLGDSGALFL
                     GGMLATISVQGAVKSAA"
BASE COUNT         4367 a         3302 c         2897 g         4284 t
ORIGIN      
        1 acaaaaaagt actataagag agcaagcttt cttaaagata taaagactga ataattatgc
       61 tggaggaaaa tactacgatg atttgcgatt aactataagc ctatcactcg cttgaaaata
      121 atataaattc attttccgca aataaggatg gagacgaaaa gataaaggca gagagagaag
      181 ctcgtgcatc atataccagt tggctgggtc ttccgcctct tttattttaa ctttttcact
      241 tgctggttca gattttttca tcctctctcc tttctccttt ttcgccaaaa cttttccgca
      301 tctgggtgtc agcctgcagg ttctgaagag aataataatc aaaaactccc aatttcccct
      361 cccttaaagc ctgagctata gctttaggaa tctctgcttc tgcttccact actttagctc
      421 gcatctcttg agtatatgcc ttcatttcct gttctctggc tacagccata gctcttttct
      481 cttcggcttt agcctgagcc actcttttat ctgcctcagc ctgatctcct tgcaggcgag
      541 ctcctatatt ctgacctaca tctatatcgg caatatcaat agagagaatt tcaaaagcag
      601 tcccggcatc taaccccttt tctagaactg ttttagaaat actatcagga ttctccaaaa
      661 cttccttgtg agagcgagaa gaaccaatgg tggtgactat tccttctccc acccgagcaa
      721 ttattgtagc ttcagtagca cctcctacta gccgatcaat attagctcta acggtaactc
      781 gagcttttac tttaagctca attccatctt tagccaccgc tgtaacctca ggagtctcaa
      841 tcacccgggg aagaacactc agctgcaccg cttccagaac gtttctcccg gcaaggtcaa
      901 tagcacaaga gcgctcaaaa gaaaggggaa tacctgctct ctctgctgca attaaggagt
      961 ttaccacccg gtctacatcg cctccggcca gataatgagc ttccaataat cctacatcca
     1021 agcctaatcc cgctttggta gccttaatta aaggaagaat aatagaagca ggttcaactc
     1081 ttcgcagccg cataccaatc aaagtagtaa tacttacctt cactcccgcc gccaaagcag
     1141 aaatccacag ccccaacgga actaaataaa ggaaaaccaa aaaagctact actacgataa
     1201 tcaaaggaac taaataattt aaaaacacca attttaccct ccttcctcag acttaactac
     1261 tatagtattt ccttttattt tttccacttt taccctggta ccaggagata taaattctcc
     1321 ctcagttata gcatcccatc ttttcccctc aatctccact attcccgccg gtcgaagcat
     1381 gcttttagct attcccgttt ttcccaccag agaaggctcc cagccttttt cttctgtttc
     1441 gtaggctcgc ccggttattt cttttctcag ccccaatctt tgccacaccg ggctttgagg
     1501 aagaaaagca agcaaaacca gaaacactcc ccccatcaga gcaatgtaga aaaacaattg
     1561 atacaaagct tcttgccaga acaaaatact ggtttcccga agaacaagat agatagagag
     1621 agctaacact cctatccccg aaatccctgt tattccaaat ccgggtagaa aaagctccac
     1681 gactaaaaga actgccccta tcaagaaaaa aataagaggc tcccaccccg ccagaccagc
     1741 aagatagcga cctccaaaaa aaagagaaaa tgaaactaag ccaataatac ctggaattcc
     1801 aaaaccagga gttaaaacct ccagcaaaat cccccctaaa gctaaaacga gaagaacagg
     1861 gactatcacc ggattagtta tcaagcgagc cagattctct gaccaagaag gagaaagttc
     1921 ccgaaccgtt ccttctaaac ccaatttgtt taataactct tcccgggtag cagctactaa
     1981 atctcccgct ccatgttcca gagcttgaga agcccgaaga gtgagaagtt cccctttttt
     2041 caccagacca tcaatttcca ggttacgatc caccatagaa gcaaaaagaa gcgggtctct
     2101 tcctcgagcc tccgccagag cttcgatttg ggctcgaata gctgatacag ttttggggtc
     2161 aggctgctct acttcccctg cagcaccact acggggctca gctgctccca tcgtggcatc
     2221 aggagcaaat acaatcgttt cacaggccag ggcaataaaa gttcctgctg accaggctcg
     2281 gtgattaatg aacgccacag tatagcaggg agaagaaata atagcatctt taataaaaag
     2341 catagcgtct accagaccac ctgaggtatc gatttccaga accactgtct gcgctccagc
     2401 ctcttctgct tcccgaaaag ctcgactcac gaaactggct aacccccagt ctaccattcc
     2461 taagtttgga acatctttca ccggaaccca aaatataacc ttttcttcgc tttgggccaa
     2521 cgtagaactt cccatcagga aaaagaggaa tattattatt cccaccaacc agtatttagg
     2581 ggcttttaac atcttttccc tccctttcct gataaattga acgtcaattt attattaatg
     2641 gtaactgaga aaaagattct ttattcccaa ttgtagtatc tcacaaaact ggttaaaatg
     2701 ttaacccaag ttgtaaattt ttaccagaaa attctgacag cctacggcgt gagattaaaa
     2761 agaactatga ggggggtaag ctctcttata gccttcaccc tcaccctaac cctctccctt
     2821 cgagggcgag ggaagaaaaa gaaaggggat tcataaaggg gaattctttc ccccttatac
     2881 tggaagagac gctttttctc ttccagtagt tttttatgct tactcttttt gcttttagtt
     2941 tttaagtctt tagtcttacg ccgcaggctg tctgcttttt gggggttata agggggttta
     3001 cccccttata gttcgggatt ataaaggggc acagcccctt tatagttcgg ggttatgagg
     3061 gggggtcttt ttccccctca tagtctttgc aatgaatttt gcgggatatt ataatcacca
     3121 gaagttatat tcagtaaagc cgaggaggac gaaagtcccg agatatttcc tcgatacgct
     3181 ggaaaagttc tttaccaatt tcaatatgga aaacttgagc tactacctga gtccagccat
     3241 ggaggaagta tatccagcca tcctctattt ttaagtcttt ttcttctctt tgagccaaag
     3301 cctggtgaat gaattttaaa tctcctcgat aattgagctc ccatactaaa ctgtgaggag
     3361 gaaactcaca attatcggtg agaggagaac caggacggtc tttacccaag cccgtagcat
     3421 taactactaa agagtaaggc ttaattctct ttaaaatctg gtcgtttatc tctgcttgag
     3481 gagaatggat gtattcggca ttaatatggg ggttaatttt tttcataatt tcttccatac
     3541 cgtaaaggcg aggggtgcta cggttagaaa cgtaaagttt gctgggcata tttccctctt
     3601 ttttagggtc aaaaagatat gcagcaatgg ctcgagcgct acctcccgcc cccataatga
     3661 aaacctctcc tccatattct ttccaaaagt taggaggaat aaaggcctcc attgctaaac
     3721 cacaagatat ggggtcttta gcatgacctt ctaaacgtcc atctctttta gagatggaag
     3781 atagctctcc aaataattga gcataagggt ctaaataatc aaataagtct ttggctgctt
     3841 catagaggtc aattttatga gtggtaacca aagctcccag agaaagaggg tcggtcttaa
     3901 taaagtttac tgcctctcga taaacttctc gaggagcatg tatctcaata tcaattccct
     3961 tgagcaccgc attatttaat cctaactcct tagcccacaa gggaaaaagc ttcataattg
     4021 aagactgggt agtggtaact ccgataaaat acatagtgga ttgagaagca cgttctggca
     4081 acttcatttt acttactcct tttaggatta tctatcagca ctacctctcc attttcttcc
     4141 accaccagtt tgggaaatcc tttttcttcg atagtagcgt aatcatgacc agcatcagcg
     4201 gggaaaatac aataaaaaat gagaggctcc tgtcctacat ttacagtgcg gtgagaccaa
     4261 aaaggaggaa tataagaagc agaaccggga aacatatcta aagcagccca ctctccatcg
     4321 gtagactcca taagcagctt tccttttcct cgcaaacaaa aataaatttc tgctgtccct
     4381 aattgttcat gcagatgtcc tttggttaga aagaattcct ttcctacttt tccgggataa
     4441 ataatagtag tgcagttacc taaatgtccg gcttcttcgg ggatgtcaac atagattact
     4501 tcataaacca gagggtcgcc attttttatc atttcctctg ctttttctct atctaggaac
     4561 atatttttaa catcacttac ccggcggatg gtctttttcc ccggggacaa ttctccagta
     4621 gaaaagtcta tgactacatt aaaaggcttc ataatatcac cctttctatt tttatgttgt
     4681 tttcattctg gaactataac caccttaaga gattgttgtt tctccataag agataaacct
     4741 tcttctattc tttccaaagg taaaacgtgg ctcacaagat aggaaaggtc taattgcttt
     4801 tgcagtagta aatctaaagc gcttcgaaaa tgaaaattat tagatttagt agttccggtt
     4861 accgtcaatt ctttgtagtg gattaaatta ctgggaagag caacttcttt tatctgagga
     4921 ggtaggccac caaagaaatt aattcttcca taaaccccgg ctacctctaa agcttccttt
     4981 tgagcatcag gagagggagc agctactact accacatctg cctcctttat cttctctttt
     5041 actttctcct tccccactac tatggtttta actcctcttt cttgggctat ctttagacgg
     5101 tcttcgctta agtctgccat ccagagctct tttgctcctc gcagtcgagc taattccaaa
     5161 aacatcaacc caataggtcc tgctccatat ataagaactc ggtctcggaa attaacccgg
     5221 agaggaagaa aaccatgcac tacacaagct aaagcttcta caaaagcaat agagaccaaa
     5281 tcagtttcct cgggaagagg aaaaatattt ccctgaaaca ctgctttttg aggaactacc
     5341 ataaactcag caaatccacc attttccgtt attcccagag ctttcccttc cggacaatga
     5401 tgagtaagac cctgaataca gtagtaacac ttcccacatc cgtaattggg agccacagct
     5461 actttcatac ccggagttaa accttctacc ccttttccta cttcctcaat tactcccacc
     5521 acttcatgcc ctaaaacacg cttttctcct tccggtatat cggcatgccc ccgccgagcg
     5581 atacgcaagt ctgtgccgca caaagctacc gctttcactc gtattagaac ttctccttct
     5641 ccaacccggg gacgaggaac ttctaccacc tgtacctttg gaggatagga aaaaactgct
     5701 gccttcaaga ttgatcacat ccttttaatt tgtttcagct aaaaagctct ttatgagaat
     5761 cgaaaacctt acccatcttt tccagagtat caagataaaa aggaaaaaga ttattataaa
     5821 aagagctcca ttcctcttgg ggaaaataag ttttaacttt ttgcactgaa tggatagact
     5881 tttccttaag gtcggaaaac aatcctactc caaaaccagc aagtagggct gctccccaga
     5941 tagcgaactc ttctctatct aactccacat aaggtatatg taagatgctg gcttttatct
     6001 tgttccagaa agatgactta gctcctccac ctatcactcg gacttctcga aagtttacct
     6061 ccggtagaag ctttctaatt atagaaaggt aataagcata ttcaaaagct actccttcca
     6121 atatagctcg gaacagatgt tctcttcgat ggtcccagct aaaaccagca aatacccccc
     6181 ggaaataggg ctcaccagga caatttctac ctcgaagatg aggtagaaaa accaaagagg
     6241 aaggggagga agcaattttt tctacctcat catcaattaa ctgaaaagcg tggggtaaat
     6301 ttgcccattc ccggaaaaat tcatctttag cccattctaa gtctaatcct cccccattta
     6361 tgtaagccat aggataataa tatccatcta acactgctcg agggaaaaga atagttttat
     6421 gttccaaatc aggaacaaac ttatctactg agagactaaa gcaggaagca gtgccagcca
     6481 cgtcaaaagc cgtgcctttt tctaccactc cagcacctaa attattagct gcctggtctc
     6541 cacaaccagc aaccaccgga gtgccaacga ggattcccat cttctctgcc cactcttttt
     6601 taactttccc taccacttgc cagggtttaa ctattagagg aaacttactt tcttccatcc
     6661 caaaaatttc catcaattct ctatcccagc agttattttc caaatcagaa aaacaggaaa
     6721 aatgaagata agtatagtct ataaaagttt cctcaccccg gagaccagaa agtttctggc
     6781 taacccagca agaaggaaca gtaaatttgt caattttttg ataaacttcg ggttgctctt
     6841 tcttccacca caggatttta ggaccatgat tgatgctggg tgccatacct gaccgtttaa
     6901 taattaattc ttcagctttg tctctcatca gctcggtata gggggcacac cttacatcta
     6961 accaggaatc ataaggggta gctgcttctc cactttcatc tacccccata atccctgcca
     7021 tttgaccatc aagagcaata gcgaggatag aagagggaga aactccactt ttttctacta
     7081 cctctcctac agtctccact actgagttca acatctcata ggggtcttga attacacttc
     7141 cgtctttgcc gtatatcaaa ttggattttt gccgggcaag agctattaaa tttccttctt
     7201 catcaaaaag accggtttta gtagccgtag ttcccaaatc actaccgatg agataaggca
     7261 aacttaccac tctccttccc aaggaactgg gctttgttga cttaaagatt tatgagctaa
     7321 ttccaccacc tttactactc ttttaccatc aataatagaa ggagatggat tttgattttc
     7381 taaaacagaa cggataaagg acttcaactc ttctaagtat ccttcccgaa aacgatctcg
     7441 ccaactggac tggttatcac accaggattt tttatccgga gtatagagaa ctggacctcc
     7501 tgcctctact tctcctactt taatcatccc ttcagttccc agaatttcca tccgagcatc
     7561 ataagcataa cctaccggac accctccatc tactactccc agggggccgt cggtaaaatt
     7621 gagagttaaa aggtagtggt cataaaaatc aggaaacttt tcttttatat ctttcccttt
     7681 aaaattttta gcctctaaag aaaggtggtg aggtttttgg ccagcgaacc acaaaactga
     7741 atctatatca tgagcagaaa cttcagctaa catccctcca ctttttgctc tatcgaaaat
     7801 ccagggagcg gggagaccag gacctcggcc ggtggattta atgagaacta catccccgat
     7861 tttcccttct cttaccattt cccaggcttt ctttatccct gggtcatagc ggcgcataaa
     7921 accaatctgg aactttatac ctgctttttc tactgcttct tgaatttttt gcacttcttc
     7981 tgagttttga gccaggggtt tttcacagaa aacgtgtttt ttagcttttg ccgctttttc
     8041 tatcatttct cgatgagtaa aagttaaaga agcaatacac acagcatcaa aatcttcttt
     8101 ttgcagagct tcatctaagg tagagtaaaa agacttaacc cccagatttt tgctcgcttc
     8161 ttcggccctc tttaaatcta catccactac cgcttttagt tctccttcgg gaatatagtg
     8221 aactaaattc tgagcatgaa ccattcccgc tcttcctgct cctaccaaaa caaaacgaag
     8281 tttatccaat gattaatcac tccttcctga gttatttata gctttttacc tctccttata
     8341 cacgttcact ctaactttac ccttgtcccc tctaaatcgg gaacggaaga aatcgattat
     8401 ttgtccctct tcggtaaagg tcacgttttc cataagaaac ataggagcac ctttctcaat
     8461 acctaaaagt tcagagtcta cttcattagc tacgatgggt tccagactta tttccgctcg
     8521 atagggcgta agaccatatt tttctgccat gatgcgataa agagattgat cagttaagtc
     8581 ttcctccatt aatccagggc aaagaactaa aggaatgtga ttattaactc tgactattgg
     8641 ctcatcttcg ataaaacgta gtcgatcgat aaaaataatt ttttcatctt cccctatctg
     8701 gagaaatcga gcaatttctt tccctgcttt tctaatctcc ctattaagga taatagtatg
     8761 cagcgaataa cctttttcga tcatatcatc ataaaaagta gtaaagtgct gaataaaacc
     8821 ataatcaatc ttgggtttgg agacaaaagt gccctttcct ttctctcgcg ttagccaacc
     8881 ttcatatact aattctttta aagcttgtct caccgtgggg cggctaattc catatttttc
     8941 agaaagttct ctctctgaaa taatagaatc cccaggatta agtttacctt cttcaatttc
     9001 ttcctttata atttctttta gctggtagta aaggggaacc ggtattttac gatttaattt
     9061 ttgctccaaa acaatcactt ctccttaaat tatattttaa agtaataata ttaagtggta
     9121 ttaaccatct aacaagagtt taaacttttt ttatataatt gtcaaggggg aactgccatc
     9181 agttagcaat aatgtgcacc agctaccctt aaagttcctt cagaatccct gatacattcg
     9241 caagtaaatg ctctaaaaat aaccgaccag ggggaagtga gaaggtaagg taaccaaata
     9301 cttcttctgc aaaagtgaat gttctaatcc ccctccaact attggctata gtatttccta
     9361 cttgaaaact cgctgtcaaa acttatcttc ttttactttg gccaagagaa aaggaggaaa
     9421 ataaaaatac tcctacaatg agttgacact atctagagcc ttcgaagctt tgacataaag
     9481 gataagcatt tgctataatt agtgcgattc taaagtagag gtgataggaa gctatgaaaa
     9541 aagaaattca tcctgaatat aaagaaactc gggtaatctg tgcctgcggt aatacttttg
     9601 ttacccgttc cactaaattt cctgaaatca aggtggaaat ttgctccaaa tgtcatcctt
     9661 tctttaccgg gcaacagaag ttagtagaca ctgcaggaag agtggagaaa ttccgtaata
     9721 aatacggaga caattattaa ttttcacctc ctcaggtagc tatcttttct agctacctgc
     9781 ttctccgatg atgaaagtaa ctcttctgaa ttatacacct gaccctgaaa ttaccgttgc
     9841 tttagctgct cggacttgtt atagcagcaa acctcctctt actatttcca aggagcgagc
     9901 agaacacctg attcgagagc tcattgagcg aggccatgaa tcagtgttag agcatgcctc
     9961 tttcactttt tttattgaag gtatatcgcg ggtaacttct caccaactgg tgaggcatcg
    10021 tttagcctct tattcccagc agagccagcg ttatgtctct atgaaagagg ccaattttat
    10081 aattcctccc tctatagctg aaaatccaga agctcaagga ctgttcctta aagctataga
    10141 ggaagataaa aaaacttacc aggaactggt aagtttggga atacctcaag aagatgcccg
    10201 ttacatgatg gcacagggtg tagctactaa cctggttctg tcagctaatg cccgggagct
    10261 attgcatatt tttaacctgc ggttatgcaa tcgagctcag tgggaaattc gagagttagc
    10321 ccaaaaaatg ttagaattag taaaagattt agctccagca atttttgaat gggctggacc
    10381 tccttgtgtg gagggagaat gtcgagaggg agagaatagt tgtggaaaac cctggaaaaa
    10441 aagagaaaaa taaagtgaaa tttgatgtag gaggacaggc tctcatcgag ggggtgatga
    10501 tgcgcagtcc ttttcgcctg gctatggcag tgcgtaagcc caacggtgat attgcactgg
    10561 aagtaaagga gcaaaaacct attactaaga gatatccttt cttatcctgg cctgtaatta
    10621 gaggaatagt gagtttgatt gattctttgg tagtgggtat caaagctctt tcttattctg
    10681 cttctctggc actcgatgag gaagaagaaa aattaagcag catggatatc ggaatttcta
    10741 ttctcctggc tctggggatt tttgtcgggt tattcgtggc tttacctacc tttctttctt
    10801 ctttagttga ccagttcata tcttccactt ttgtttataa cctggtagag ggattaattc
    10861 gtatagggat ttttttagcc tatttattat tgatatcaca aatgaaggac atcaaacggg
    10921 tgtttgaata ccatggagca gagcataaag ctatttttac ctgggaaaac ggtgaggaac
    10981 ttactccaga aaatgcccga aaatttacca cccttcatcc tcgttgtgga actaactggt
    11041 taattatagt attaataatc agtatattcg ccttctcttt tctgggtaga ccaggattaa
    11101 ctatgagggt ggtaagccgg atattgctta ttccattggt ggcaggcctg gcttacgagg
    11161 cgattaagtt cttatcccga tatcaggaca atcatttagc ttatatttta accttgcctg
    11221 gtttacttct acagcgcatc accactcgag aaccagatga cagccagtta gaagtagctt
    11281 ttaccgcttt aaaaggtagt ctaagggagg ataattatgt ggcgggagaa aatagaggaa
    11341 atagttaagg agtatcagga tttaactcaa aaaatagcca atccccaatt gatgaaggat
    11401 atggctactt accgaaattt aactcaaact ttagttgaaa tagagcctgt agtaaaaaaa
    11461 tatgaagaat atgagaaaaa agaaagagaa ctggaagaga accgggagct tctccacaca
    11521 gaaagtgacc cggagctccg catcctttta gaagaagaga tagaaaagtt gaaaacctcc
    11581 cttccagaat tggaagaaga acttaaaaca atgctcatcc ctcgggaccc ccgagatgaa
    11641 aaaaacacct tggtggagat tcgagccggc acgggaggag aagaagcagc tttatttgta
    11701 gctgatcttt tccggatgta tagtcgttat gcggagcgca atggatggaa aatagagata
    11761 atgagctcta acccaacgga gctgggagga tttaaggaaa taatttttgc tgtagaggga
    11821 aaaggagcct atagtaagtt taagtttgaa agcggggttc accgggtaca gagagtcccg
    11881 gtaactgaag caggaggacg cattcatacc tctacagcta ctgttgcagt tctcccggaa
    11941 gccgatgagg tagatgtaga gattaaaccg gaagatatac gagtagatgt gtatcgttct
    12001 tcaggtccag gaggacaaag tgtcaacacc actgattctg ctgttcgggt tactcatatc
    12061 cctacgggaa tggtggtaac ctgccaggat gaaaaatccc agcataaaaa taaagctaaa
    12121 gctttaagaa ttctacgtag ccgtctgctg gaaatggaaa gagaaaacca gaaagaagag
    12181 atagaccagg aaagaagaac acaaatcggt agcggagagc gaagcgagag gatccgcact
    12241 tataacttcc cccagaatcg ggtcactgac caccgaatta atttaactct ttatcgtctg
    12301 gaatcggttt tagaagggga gctggatgaa ataatcgaag ctttagcagc agaagaaaaa
    12361 atgagaacgc tggaaaaagt gagttaaatg ccctcagtta aagaggtgtg gcagaaatta
    12421 gtaaaagatt tgaaagagca ctcttttctc tctccgggaa gagaggctcg tttgctttta
    12481 gcttctgttt taggagtaga accctctcga gtttatcttt tctgggatac agaactctct
    12541 ggagaaaaat taactcgcct gcatagcatc ctcgaaaaac gccggcaagg tgttcccatc
    12601 caatatcttg ccggggaatg ggaattcttt tctcttcctt ttcgcttaaa aagaggagta
    12661 tttatccctc gcttagacac cgagagctgg gtagaggaag ctattgttta ccttcgttcc
    12721 ttaccggaag aaaaaataat aatttgcgat atgggctgtg ggagtggagt aattggcata
    12781 tcttgtgctt tctgtataga aaacttatat ttatatggag cagatatttc tccccaagca
    12841 gtgaagctat cagagaaaaa tgctcacctt ctaggagtag ataaaaggag tgcttttctt
    12901 ctctctgacc ttttttctgc tttccctcct cttaagttcg acgttatcct ttctaatcct
    12961 ccctatatca gagagaaaga ttgggactct ctacctcctg aagttaggct ttatgaacct
    13021 cgggaggcac ttcttgctgg agaagagggg ttagatataa taggaagaat tttagaagaa
    13081 ggagaacctt tcttaaaaga agaagggctg tttttcttag aacatgaccc ggagcagaaa
    13141 gaagctatcc agaaattagc cgatgctctt tccctggaat atttacgttc cattcctgac
    13201 cttacgggaa aagaaagagc cagtgtaatt cgaaaaagga ggagcaaaaa tgaaagtact
    13261 cctaggtagc gaccatggtg gttttgaatt aaaggaagac cttaaagtct ttctcgaaga
    13321 actgggacac gaagtggaag attatgggac ttataatacc caaccttgcg attaccccga
    13381 tgtggctttt caggtagcag aagatataat aaaaggaaag ggagaaagag ggattttaat
    13441 ttgcggaacc ggtataggta tgtccattgc tgctaataaa gttaaaggaa taagggcctc
    13501 actatgccat gatacttttt ctgcccgttt tgcccgggaa cacaacgatg ctaacattct
    13561 caccttaggg ggaagagtgc tggggaaagg tctggcccga gaaattgttc gagtatggtt
    13621 agaggctaat tttactggag aaaggcatgc ccgccgggtg aataaaatag aggaatatga
    13681 aaagaatgag agatagaccg gattgggata cctattttat gtccattgct caccttgtga
    13741 gcactcgctc tacctgcttg cgccgtcagg taggagcagt tatggtgaga gataaacaaa
    13801 tcttaaccac cgggtataat ggagttcccc aggggataac ccattgcact tcagagacct
    13861 gtttgcgtag cttaaaaaat attccctccg gccagcagca agaattatgt cgaggattgc
    13921 acgctgaaca gaatgccata atccaggcag ccctgcacgg tgtaagtact aaagaagcca
    13981 ctttatattg cactcacaaa ccttgcattt tatgtgctaa aatgttaatt aacgctggag
    14041 tagcgagaat agtgtatcaa aatttctatc ccgatccttt agccgatcag atgttagaag
    14101 aggcaggaat agaaatgtgt atctgggagg ggcaggtgat atcttattga aacaggtttt
    14161 atttttcagt tttctgtctt gtattttagc tctggccata gttcctttag ttatccgatt
    14221 cagtaaaaag aaaaaatggt ttgacatacc agatgggaag aggaaaatcc acactaaacc
    14281 tgttcctcgc ctgggaggaa ttgccttatt tcttgctttc tttttggctg tttttattat
    14341 ttctcttcgg ggtagttttc cttttccctt tttccttttg gcaggagggg gagtaatatt
    14401 tctcaccggt ttaatagatg atcttttatc acttcctccc tggggaaagc tcataggaca
    14461 aatcgcgggc gctctcattc ttattgcagg aggagttacc attcaatttt ttacccttcc
    14521 ctgggataaa atggtttacc tgggcagctg gggttatcct ctgacgttat tgtggatagt
    14581 agggataacc aatgctttaa acctcatcga tggtatggat ggcttatccg ctggtatagg
    14641 agctatcgct tctttcactc tgggagtcat tgcctggcag gagggaagaa tagagccggc
    14701 ggctttatcc tttcttcttg caggagctac cattggattt ttattctata attttcctcc
    14761 agcaaagatc tttctgggag atagtggtgc cttattttta gggggaatgc tggctaccat
    14821 atcggtacag ggagcagtaa agagtgctgc
//
LOCUS       DAOSVE010000036        14629 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_2487_length_14629_cov_68.916221, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000036 DAOSVE010000000
VERSION     DAOSVE010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 14629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14629
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2487_length_14629_cov_68.916221"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..1280
                     /locus_tag="PK016_04460"
     CDS             156..1280
                     /locus_tag="PK016_04460"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HXL02356.1"
                     /translation="MRDTYRIKKIEIPLSELEIGEILPFTLLNYQDQVLLGRGRIITS
                     EFMEKLKKDGYSSIVAEILEEDEGLVAKVDISPEIKKETLENLQGVLEDFSRGKTPSI
                     VPLERNVSQMTEEVKAHKELVVPLIQLKKHDDLTFTHSLNVAVISLFIAKFLNLDGGE
                     LTNLGLGALLHDLGKLLIPPEILNKPSKLNEKEYEIVQKHTLFAERILDEKTQLPEEV
                     KKVPLQHHERIDGSGYPFHLTKEEISIFSRIVAVADIYEALTSDRPYRKALPIPEVIE
                     YLMGNNGYRLDDRVVTTFICHLSPYQVGDLIKLSTGEEAVVSRINPTLPFRPIIRIKR
                     EDERGKIYFSEEIDLSHSLTLTIISGIDMKSQIDVERAIL"
     gene            1280..2065
                     /locus_tag="PK016_04465"
     CDS             1280..2065
                     /locus_tag="PK016_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXL02357.1"
                     /translation="MESFIKFLGTAGARIVVSKQLRASGGVWIKYQNTNLHLDPGPGA
                     LVKALSSRPPLSPSSIDAVLLSHRHLDHSNDLNIMVEAMTEGATRKKGAVFLPREALE
                     VEPVLFSYLREKLVRLEILEEGKDYHWKDISFSTPLRHLHSAETYGFRFSFPQGDVSF
                     ITDTLFEEKLIEAYQGSKILFLNTVRFKKDSHPSILHLSIPDAQTLIENIKPRLAFLT
                     HFGLTVLREKPWKIAEEMSQKTGIKVIAASDGMKIDLEEVLRD"
     gene            complement(2062..>2732)
                     /locus_tag="PK016_04470"
     CDS             complement(2062..>2732)
                     /locus_tag="PK016_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02358.1"
                     /translation="ETFDFWRELKKNWNLIIRYPQILLVTLIPSLAIAASNYLTWQGH
                     LAWVSIFFREAGSAILLIVGVFLSFIALSLITAIAWDYRYRDVIDLRRAWRIIQKRLP
                     DIVMVSLIMGFLEGFFAIWFVFLGLVFAFLLLFTIPLTVVEGDNPFSAIRNSFHLVSE
                     NLGECFTFFIIALFFLLIGYLLFWLLGFLGIVGLILNTVVGGLILAYISLLLSSFYFS
                     LTRF"
     assembly_gap    2733..2832
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2833..3862
                     /locus_tag="PK016_04475"
     CDS             <2833..3862
                     /locus_tag="PK016_04475"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXL02359.1"
                     /translation="LFIPLFLVCLINSGGIVLSLEPLDQAQWLLEKGETDKVLSLLEP
                     LLDSFSEEELSQAVEIIYQIYLEKGESDKAIQTLEKYINRFPGTPYSYLYRYWIAKIE
                     EERGNFNKALELFYQIVNSYPPEVEDPYELKNLVREDIAYNLQYYHKNYLEAIKVYQE
                     LLDSYSGVEEKARVMMEIASCYEKLGKIEEAISYYKKVTREAPDFSYGRWAELRIVYL
                     QSQPTGVEKSKEALVQKLKEAFQSKDIKKLETLAKKGDFWVGVMFSEFEVDDFNKVEN
                     YLSKYLPQSNNLQIGELEKRNDDFTLRIDNWPDPDYNILYLLIEEGLYGWEWKGVILS
                     NTELESLI"
     gene            3869..4465
                     /gene="hisB"
                     /locus_tag="PK016_04480"
     CDS             3869..4465
                     /gene="hisB"
                     /locus_tag="PK016_04480"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HXL02360.1"
                     /translation="MVKLAKRETLVKRQTRETDIEIYLSLDGGKSIQMDMAVPFFPHL
                     LESMAFYANWDLNIKATAQEFMDDYHHLIEDTGIVLGEALNKCLGDKKGIERFATVFI
                     PMDEALSMVAVDLSGRPYFLWEKPDLGERVGSFEIMLVKEFLQALVNNAKITLHAKIW
                     WGENAHHCLEALFKALGKSLAQAAKITSIYLPSTKGTL"
     gene            4481..5089
                     /gene="hisH"
                     /locus_tag="PK016_04485"
     CDS             4481..5089
                     /gene="hisH"
                     /locus_tag="PK016_04485"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HXL02361.1"
                     /translation="MIAIVDYEIGNLYSISKALERLGEKVVVTNQAQVIVEAEAVVLP
                     GVGSFGKAMENLEKAEIKEAIRKNQESGKPLLGICLGLQLLFEKSQEAPRKKGLSLLP
                     GEVVKLPPTNKIPHVGWNRVYFKKDSPLTRDVPQGRFFYFAHSFYVLPEDFQTVVGIC
                     NYNVAIPAIVNQDNIWGFQFHPEKSSVWGMKILENWVRSWKK"
     gene            5041..5814
                     /gene="hisA"
                     /locus_tag="PK016_04490"
     CDS             5041..5814
                     /gene="hisA"
                     /locus_tag="PK016_04490"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HXL02362.1"
                     /translation="MGYENPGKLGEELEEVILPIPAIDVLEGKVVRLEKGDYQKAEVF
                     GTSPLLVAQRWEKEGASLLHVVDLEGAKSGYPVNFSVIKELIKGVSIPVEVGGGIREI
                     SSLLGYLDAGAERVVLSSVVVKNPSVFEEMLGIARERIVVSIDTRDQLVSLEGWKEEI
                     PVKAVDLARALREKGIEHFIFTDVQRDGTLQGVRVEVIRNFIIGSGVNIIVAGGISCR
                     EDIRKIKSLGEGIEGIILGKALYSGQLNFKEVCNILREE"
     gene            5817..6596
                     /gene="hisF"
                     /locus_tag="PK016_04495"
     CDS             5817..6596
                     /gene="hisF"
                     /locus_tag="PK016_04495"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HXL02363.1"
                     /translation="MLARRIIPCLDVKEGKVVKGVHFENIKDVGDPVELAQRYEEEGA
                     DELVFLDITASYEQRGTMIEVVEKIAEVLTIPFTVGGGIGATGQVANLLKAGADKVSI
                     NTQAVLNPELISELAEMFGSQCVVVAIDVKRTWDRINRRSYWEVFINGGRTPTGKDAL
                     SWAQQVEKLGAGEILLTSMDTDGTQTGYDISLTRKVAERVRLPIIASGGAGKLEHLLA
                     VLREGRADAALVASIFHYRYFSIGEAKDYLQKRGIPVRIED"
     gene            6596..7252
                     /gene="hisIE"
                     /locus_tag="PK016_04500"
     CDS             6596..7252
                     /gene="hisIE"
                     /locus_tag="PK016_04500"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HXL02364.1"
                     /translation="MEKTWEDAFFEKEELIPAIIQDVESGKVLMMAYMNREALSRTLE
                     TGKTWFYSRSRKQLWNKGESSGHFQEVQGIYVDCDNDTLLIQVRQKGAACHTGFFSCF
                     HREVRKGEISEPEPQFPPYSLSSFLQELSEVIEERAVNPSPQSYTSRLLEEGREKILR
                     KVAEEATEVILATFEDEDRNREHLRWEVADLLYHLLVLLQHEEVTYYQIMEELKKRRK
                     "
     gene            7373..9256
                     /gene="aroF"
                     /locus_tag="PK016_04505"
     CDS             7373..9256
                     /gene="aroF"
                     /locus_tag="PK016_04505"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HXL02365.1"
                     /translation="MIVIFNRKLNDLEKSELREKLEKENLNPYFLEEETGKTVLITEN
                     KFPTSLLDEKVIERTMEIDTPFQLVHRSFRPQGTVIKVKGVEIGGDNLTVMAGPCAVE
                     SEEQLFETAEIVKRAGVHVLRGGAFKPRTSPYSFQGLGEKGLEILARAGEKFGLPVVT
                     EATSPEIADLVAQYADIIQIGARNMQNFELLKKVGFLKKPVLLKRGLSSTIEDWLLAA
                     EYILSWGNFQVILCERGIRTIEKFTRNTLDLTAIPVVKDLSHLPVIVDPSHGTGLREK
                     VIPMARAAVACGADGVMVEVHPHPEKALSDGPQSLYPEQFKKLMNDLEVISILVGKEL
                     KKSDIPERLEVKSKPGPLNVRVAFLGERGSFSSQVARRTFGERAELLPCSNFREIFEK
                     VKQEEATYGLIPIENTITGSIHNNYDLLLEFPDLYVVGERFIRVSQHLGLYPGTKKDE
                     IQVLFAHPQGFAQCARFIESLQGVRVINVGSTEEAARRTGEFGIGGACVSSEEAIKKY
                     HLELKEEEIEDNPRNFTRFLIISSYFSPSSQDDKVSCVFAVNNYPGALYEALQVFAVR
                     KVNLLKLESRPRPGKPWEYLFYVDWEGNLKENIYEDMIKELSTKTSFWRILGSYKNSW
                     KKS"
     gene            9269..10200
                     /locus_tag="PK016_04510"
                     /pseudo
     CDS             9269..10200
                     /locus_tag="PK016_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873546.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
     gene            10420..11751
                     /locus_tag="PK016_04515"
     CDS             10420..11751
                     /locus_tag="PK016_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HXL02366.1"
                     /translation="MEERKSFEERELFQIQEKEVKPLASRMRPRSLDEVVGQPHLTGK
                     EGILRKIVETGFLPSLIFWGPPGSGKTSVAYLVAKACKYQFISISAVTSGVKEIREAV
                     KEAENDLKFHRKKTVFFIDEIHRFHKGQQSLLLPHVEEGIVTLIGSTTENPSFEIIAP
                     LLSRSQVLVFQKIPVEDLVYLLKKTLEDERGLKDEKIKIEEDALFFMAQLADGDARQA
                     LNLLEVASRIAQQENKGEIGVKFLQDTFHKTSYLYDKSGEEHFNLLSAYHKSLRGSDP
                     DAAIYWLVRMLEGGEDPHAILRRLVACASEDVGNADPHALLIAVAAQEAYDFLGEPEG
                     RLSLAQATIYIASAPKSNASYQALNQALADVREYGALPVPLHLRNAPTGMMKEMGFGK
                     EYRYPHDFNGAFIEQEYLPPQLQGKTYFHPHDRGYEKTIGERLRLWWKNRK"
     gene            11958..12110
                     /locus_tag="PK016_04520"
     CDS             11958..12110
                     /locus_tag="PK016_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin FeS4 iron-binding
                     domain-containing protein"
                     /protein_id="HXL02367.1"
                     /translation="MPEKKETYRCNACGNVVEVEESEKEDLTCCGEPMVKVEEEKPKG
                     GGCCRK"
     gene            complement(12516..13490)
                     /locus_tag="PK016_04525"
     CDS             complement(12516..13490)
                     /locus_tag="PK016_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019669330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXL02368.1"
                     /translation="MRTRKLGWTDLELTVIGFGSWALGGGGWQFSWGPQDDEESIAAI
                     HRALDLGINWIDTAAVYGLGHSEEIIAKALKGMSKKPIIATKCGRVWNEKGEVFGCLK
                     RDSIRREVEDSLRRLEVEVIDLYQIHWPDPDPDIEEAWSTMADLVKEGKIRYAGVSNF
                     NVEQMKRIEKIHPIASLQPPYSMITRDIEKEILPYCAEKNMGVIVYSPMQKGLLTGKF
                     TRERVQNLPPDDHRRSDPQFNEPLFSINLELVEKLKPIAEKYGRTLAQLAIAWVLRRP
                     EITAAIVGTRRHSQIEETFPAGDWELTTEDIKAIDQLLQEREEKIKNL"
     gene            13647..13784
                     /locus_tag="PK016_04530"
     CDS             13647..13784
                     /locus_tag="PK016_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02369.1"
                     /translation="MAIITMLNNGLQLAGIHQIWQVGALGLALIGSVLLNQFLIRRRK
                     I"
     gene            13799..14161
                     /locus_tag="PK016_04535"
     CDS             13799..14161
                     /locus_tag="PK016_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02370.1"
                     /translation="MLVIILVLGGESLLGKETSQVFLLSPSLDYFPAFLEVHLRRLLS
                     PSQVHLVTLPQEELPRGDVSLLVFLGKEQYFSSILLQIPQNKKPLFLFWERKEIEPSS
                     GFSFCKIRVEGEEIIEAL"
     gene            14264..14536
                     /locus_tag="PK016_04540"
     CDS             14264..14536
                     /locus_tag="PK016_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02371.1"
                     /translation="MISDKEKSLVIAENTEAGRQLIDEFSEAESPPPIVVLEASPEML
                     FTLLQGKIKAVVDFKPSQLAQEIVNLMDCFGAHKPIPEFILSSILA"
BASE COUNT         4431 a         2451 c         3341 g         4306 t
ORIGIN      
        1 gatactataa ttttatgagc aaagcaggtt ttatctaaat aaggtaaaaa ataaaggaga
       61 gatactaccc atatagacgt ttttaaagta tttaatttaa actaccggaa aaaatggtta
      121 taattgtggt ggaggataaa aagtggagga taaaattgag agatacttat cgaataaaaa
      181 aaattgaaat tcccctctcg gagctggaga taggggagat tcttcccttt actcttctta
      241 attaccagga tcaagttctt ttgggtagag ggcggataat aacttctgaa tttatggaga
      301 aactgaaaaa agatggttat tcttctattg tggctgagat tttagaagaa gatgaagggc
      361 tggttgctaa agtagacata tctccagaga ttaaaaagga aactctggag aatttacagg
      421 gggttttaga agatttttct cgagggaaaa ccccttctat tgtacctctg gaacggaacg
      481 tttctcagat gaccgaggaa gttaaagctc ataaagaact agttgtacct cttatacaat
      541 tgaagaaaca tgatgacctt actttcaccc attccctcaa tgtggctgtt atttccttgt
      601 ttattgctaa gtttctaaac ttagatggtg gagaacttac caatctgggt ttgggtgccc
      661 ttttgcacga tttaggaaaa cttttaattc ctccggaaat tttgaataag ccttctaaac
      721 taaacgagaa agaatatgag attgttcaaa agcacactct atttgcagaa agaatcctgg
      781 atgagaaaac acaactacct gaagaagtaa aaaaagtgcc tttacagcat catgagagaa
      841 tagatggtag tggctatcca tttcatttga ccaaagaaga aatttctatt ttctctcgga
      901 ttgttgcggt agcagatatt tacgaagcac taacttctga ccggccttac cgaaaagctc
      961 ttcccattcc tgaggttatt gaatatttaa tgggcaataa cggttaccgg ctggatgata
     1021 gagtggtaac cacttttatt tgccatcttt ctccctatca ggtaggagat ctgataaaat
     1081 tatctactgg ggaagaagcg gtagtaagca gaatcaatcc cactcttcct ttccgaccta
     1141 taattcgtat taaaagggaa gacgaacggg gaaagattta tttctcagaa gagattgacc
     1201 tttctcattc cctgactctg actataatta gcgggataga tatgaagagt cagatagatg
     1261 tggagcgggc tattttataa tggaaagttt tatcaaattc ttaggaacag ctggagcccg
     1321 aatagtggtt tctaaacagc tacgtgcctc aggaggagtg tggattaaat atcagaatac
     1381 taatcttcat ctcgaccccg gtcctggagc tttagttaaa gccttatcca gccgtcctcc
     1441 cctttctcct tcttctatag acgctgttct tctttcccat cgtcatctgg accactctaa
     1501 tgacctcaat attatggtag aagctatgac tgaaggtgct acccgaaaaa aaggggctgt
     1561 ttttcttcct cgagaagctt tagaggtgga gccggtgcta tttagctatt tacgggaaaa
     1621 gttggtaaga ctggaaattc tggaagaggg taaagactat cactggaagg acatttcttt
     1681 ctctactcct ttgcgtcacc tgcactctgc tgaaacctat ggttttcgtt tttcttttcc
     1741 ccaaggcgat gtatctttta ttaccgatac tctttttgaa gaaaaattaa ttgaagctta
     1801 tcaaggtagt aaaattttat ttctgaacac tgtccgcttt aaaaaggata gtcacccttc
     1861 tatactccat ctgagtattc ctgatgctca aactctcatt gagaacataa agcctcgttt
     1921 ggctttcctc acccattttg ggctgactgt tttgcgagaa aagccctgga aaatagctga
     1981 agaaatgtct caaaaaacgg gtataaaagt gatagcagct agtgatggga tgaaaataga
     2041 cttagaagaa gtattgagag attaaaaacg agtcaaggaa aaatagaagg aggagagtaa
     2101 aagagaaatg taagctaaga ttaatcctcc tactacagtg tttaatatca gtcccactat
     2161 tcctaaaaag cctaaaagcc agaatagaag ataacctatc aggagaaaaa atagagcgat
     2221 gatgaaaaaa gtgaagcatt ctcccaggtt ctcgctaaca agatggaagc tgttacggat
     2281 agcagaaaaa ggattgtctc cttcaaccac tgtaagggga atagtaaaaa gtaatagaaa
     2341 ggcaaacacg agtcctaaaa atacaaacca gatagcgaaa aatccttcta aaaaacccat
     2401 tatcagggaa accatcacta tatctggtaa gcgcttttga attattcgcc aggctctgcg
     2461 caaatctatg acatctcgat agcgataatc ccaggctatt gctgttatta aactcaaagc
     2521 aataaaactc aggaaaaccc ccacaattaa taaaattgca cttcctgctt ctcggaaaaa
     2581 aatagaaacc caggcaagat gcccttgcca ggtcaggtag tttgatgcgg caatcgctag
     2641 agaaggaata agagtaacga gaagtatctg agggtaacga atgataagat tccagttttt
     2701 cttcagctct cgccaaaaat caaatgtttc cannnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nntttgttca ttcccctttt tttggtttgc ttgataaata gtggggggat
     2881 tgttttatct ttagagccat tggatcaggc tcaatggttg ctggaaaaag gagaaactga
     2941 taaagtcctt agtcttcttg agcctcttct ggactctttt tctgaagaag agctatccca
     3001 ggcggtagaa ataatttatc agatatattt ggaaaaagga gaaagtgata aggctatcca
     3061 aacattagaa aaatatatca atcgatttcc tggcactcca tattcttatc tctatcgcta
     3121 ctggatagcc aagatagaag aggagagggg gaattttaat aaggcgctgg aactatttta
     3181 ccagattgtt aattcctacc ctccggaggt ggaagaccct tacgagctaa aaaatttagt
     3241 cagagaagat atagcttaca atcttcaata ttatcataag aactatcttg aggctattaa
     3301 agtttaccag gagcttctgg attcttattc cggtgttgag gaaaaagcaa gggttatgat
     3361 ggagattgct tcttgttatg agaagctggg caaaatagaa gaggccattt cttattataa
     3421 aaaagtaacc cgggaggcgc ctgatttctc ttacggtcgg tgggcagaat tgcgcatagt
     3481 ttatcttcaa tcacagccca ccggggtgga aaaaagtaag gaagctctgg ttcaaaaact
     3541 taaagaagcc ttccagagta aagatattaa aaagcttgaa actttagcta aaaaaggaga
     3601 tttttgggtg ggggttatgt tcagtgaatt tgaggtagac gattttaata aggttgaaaa
     3661 ttacttgagt aaatatcttc ctcaatctaa caatcttcaa attggggagt tagagaaaag
     3721 gaacgacgac tttactctaa gaattgataa ctggccagac cctgattata atatcctcta
     3781 tcttttaata gaagaaggtt tatacggttg ggagtggaag ggcgtcattc tttctaatac
     3841 cgagttagaa agtctaatat aacaaggagt ggttaaattg gccaaaagag agactctggt
     3901 taaaagacaa actcgagaaa ctgatattga aatttatctc tccttagatg gtggaaaaag
     3961 tatccagatg gatatggctg ttcctttttt cccccatctt ttagaaagta tggcttttta
     4021 tgctaactgg gatttaaata taaaggctac tgctcaagaa tttatggatg attatcacca
     4081 tctgatagaa gatactggta tagttttggg agaagctctg aataaatgtc tgggagataa
     4141 aaaggggata gagcgttttg ccactgtttt tattcctatg gatgaggcgt taagcatggt
     4201 ggctgttgat ttaagtggaa gaccctactt tctctgggaa aagcctgatt tgggagagag
     4261 agtgggtagc tttgagataa tgttagtaaa agaattcctg caagctctgg taaataatgc
     4321 aaaaattact cttcatgcta aaatatggtg gggtgaaaat gctcatcatt gtttagaagc
     4381 tctgtttaaa gccctgggta aatctttagc tcaagctgct aagataacga gtatttatct
     4441 tccttctacc aaaggaactt tatgaaaaag aggagtagcg atgattgcta ttgttgatta
     4501 cgagatagga aatttatata gtatttctaa agccctggag aggttgggag aaaaagtagt
     4561 ggttactaat caagctcaag tgattgtaga agctgaggca gtggttctcc cgggtgtggg
     4621 ttcttttgga aaagctatgg aaaatctaga gaaagcggag ataaaagaag ctattagaaa
     4681 gaatcaggaa agtggtaaac cccttttagg gatatgctta ggtttgcagc tcctttttga
     4741 aaaaagccaa gaggctcctc ggaaaaaagg gctttctctt ttgccgggag aagtagtgaa
     4801 gttgcctcct accaataaga taccccatgt aggctggaat cgagtatact tcaaaaaaga
     4861 ttcccccctt acccgggatg taccgcaagg gagatttttt tattttgctc attcttttta
     4921 tgttttaccg gaagacttcc aaacagtggt gggaatatgc aactacaatg tagcgattcc
     4981 tgccattgtg aatcaggata acatctgggg cttccaattt catcctgaaa aaagttcggt
     5041 ttggggtatg aaaatcctgg aaaactgggt gaggagctgg aagaagtgat tttacccatt
     5101 cctgctatag atgttctgga agggaaagtg gtaaggctgg aaaaagggga ctatcaaaaa
     5161 gcagaggttt ttggcacctc tcctcttttg gtagcccaga ggtgggaaaa agaaggagct
     5221 tctcttttac atgttgtaga tttagaggga gcaaaatcag ggtatcctgt taatttctcc
     5281 gttattaaag agttaattaa aggagttagt ataccggttg aggtgggagg agggatcagg
     5341 gaaatttctt cccttctggg ttatcttgat gccggagcag agagagtagt cctcagtagc
     5401 gtggtggtta agaatccctc ggtttttgaa gagatgttag gaatagcaag ggaaagaata
     5461 gtggtgagta tcgataccag ggatcagctt gtctctttag agggatggaa agaagaaatt
     5521 cctgttaaag ctgtggattt ggctagggct ttaagagaaa agggaatcga gcattttatc
     5581 tttaccgatg ttcaaagaga tggaacgctt caaggagtga gagtggaagt gattagaaat
     5641 tttattattg gaagtggagt aaatattata gttgcgggag gaataagttg tagagaagat
     5701 atccgaaaga taaagagcct gggagagggt atcgaaggaa taatcttagg taaagctctt
     5761 tatagtggac agcttaattt taaagaagta tgtaatattc tacgggagga ataaaggtgt
     5821 tagccagaag aattatcccc tgtttagatg tgaaagaggg aaaagtggta aaaggggttc
     5881 actttgaaaa tataaaggat gtaggagacc cggtagagct ggcccagaga tacgaggaag
     5941 aaggagcaga tgaactggtt tttctggata taactgcttc ttatgagcag agaggaacta
     6001 tgattgaagt ggtggaaaag atagctgagg ttttgaccat tcctttcaca gtgggaggag
     6061 gaataggagc tactggacag gtagctaatc ttctcaaagc tggagcagac aaagtatcga
     6121 ttaatactca ggcggttctt aatcccgagt taattagcga gttagcagaa atgtttggca
     6181 gtcaatgtgt ggtagtggct atcgatgtta aaagaacctg ggaccgaata aatcggcgca
     6241 gttactggga agtgtttata aatggaggaa ggactcccac cggaaaagac gccttatcct
     6301 gggctcagca ggtggaaaag ttaggagcgg gagaaatatt gttaaccagt atggatactg
     6361 atggaactca gactggctat gacatcagcc ttacccggaa agtggcagag agagttcgtt
     6421 tacctattat tgcctcgggt ggagcaggga agctggagca tcttttggct gttttgagag
     6481 aaggtagagc tgatgcagct ttggtagcct ctatttttca ctaccgctat tttagtatag
     6541 gagaagctaa ggattacctc cagaaaaggg gaataccagt aaggatagaa gactgatgga
     6601 aaaaacttgg gaagatgctt tttttgaaaa agaagaatta attcctgcta tcattcaaga
     6661 tgtagaaagt ggcaaagttt tgatgatggc gtacatgaac cgggaagcat tgtctcgtac
     6721 tttagagaca gggaaaacct ggttttatag taggagtagg aaacaactgt ggaataaagg
     6781 agaaagcagc ggccattttc aggaagtaca gggaatttac gtagattgtg ataatgatac
     6841 attacttatt caagtaagac aaaaaggagc agcctgccat actggttttt tttcttgctt
     6901 ccatagagaa gtaagaaaag gagagatttc ggagcccgag cctcagtttc cgccttattc
     6961 tctttcttct tttttacagg aactttctga agtgatagag gaaagagctg taaatccttc
     7021 acctcaatct tatacttctc gccttctgga agagggaaga gaaaaaattc tgcgcaaggt
     7081 agcagaagaa gctactgaag taatactggc cacttttgag gacgaagacc gtaatcgaga
     7141 acacttgagg tgggaagtag ctgacctcct ttatcatctt cttgttcttt tgcagcatga
     7201 agaagtgact tattaccaga ttatggaaga attaaagaaa agaaggaaat aagacagggg
     7261 ataaatatca gccctttctc cttttctacc ttctcttacc agaattgaat aaaattatgt
     7321 gtatttcgat atccagtaag tagtggttta ttagcttgag gggtgaaaca ctttgattgt
     7381 catttttaat cggaagctga acgacttaga gaaatcagaa ttaagggaaa agctggaaaa
     7441 agagaattta aatccttatt ttctggaaga ggaaacagga aaaaccgtct tgattactga
     7501 aaataaattt cctacttcgt tgctggatga aaaagtaata gaaagaacaa tggagataga
     7561 tacccccttt caactggtgc atcgtagctt tcgccctcag gggacagtta ttaaagtaaa
     7621 aggagtggaa attggaggag ataatctcac ggttatggct ggtccctgtg ctgtggaatc
     7681 agaggaacag ctttttgaaa ccgcagaaat agtaaagaga gcgggagtcc atgttcttcg
     7741 aggaggagct tttaagcctc gcacttcccc ctatagtttt cagggtctgg gagaaaaagg
     7801 gcttgaaatt ttagcccggg caggggaaaa atttggttta ccggtggtta ctgaagctac
     7861 cagtcctgag attgctgacc ttgtggcgca atatgctgat attattcaga ttggggctcg
     7921 taatatgcag aattttgagc ttctaaaaaa agtaggattt ttgaaaaaac cagtacttct
     7981 caagcgaggt ttatcttcta ctatagaaga ttggttatta gctgccgaat atatcttgag
     8041 ctggggaaat ttccaggtca ttctctgtga gcggggcatt agaaccattg aaaaatttac
     8101 ccgtaatact ctggatttaa ccgctattcc ggtggttaaa gacctttctc atttaccagt
     8161 gatagtagac cccagtcatg gaacaggcct gcgagagaaa gtgattccta tggctcgagc
     8221 agctgtagct tgtggagcgg atggagtgat ggtggaagtc catccccatc ctgaaaaagc
     8281 tctctctgat ggtccgcaga gcctctaccc tgagcaattt aaaaagttga tgaatgacct
     8341 agaagtgata agtattctgg tgggtaagga gctgaaaaaa agtgatatac cggagaggtt
     8401 ggaggtaaaa agtaaacccg gacctttgaa tgtgcgagta gcttttctgg gagaaagagg
     8461 ttcttttagc tctcaagtgg ctcgtcgcac ttttggagaa agagctgagc tattgccttg
     8521 ttctaatttt cgagagatat ttgaaaaggt aaagcaagaa gaggctactt atgggttgat
     8581 acctatagaa aataccatta ccggtagcat tcataataat tatgaccttc ttctggagtt
     8641 tcctgatctt tatgtggtgg gggaacgatt tatccgcgtt tctcagcatt taggtcttta
     8701 tcccggtact aaaaaggacg aaattcaggt attatttgct caccctcagg gttttgctca
     8761 gtgtgctcga tttattgaat cgttacaggg agtgcgagtg ataaatgtag gtagtactga
     8821 agaagcggct cgtaggaccg gggaatttgg aataggagga gcatgcgttt cttctgaaga
     8881 ggccataaaa aaatatcacc ttgaattgaa ggaagaagag attgaagata atccccgcaa
     8941 ctttacccgt tttttgatta tttcttctta tttttcacct tcttctcaag atgataaagt
     9001 ttcctgtgtt tttgctgtaa acaattatcc tggggcgctt tatgaagcac ttcaagtttt
     9061 tgctgttcgg aaagttaatc tattaaaact tgaatctcgt cctcgaccgg gaaaaccctg
     9121 ggagtatcta ttttatgtag actgggaagg aaatttaaag gaaaatatat acgaggatat
     9181 gataaaagaa ctatccacca agaccagttt ctggcgtatt ctgggaagtt ataaaaattc
     9241 ctggaagaag agctaaagag gaaggaatat gaaaaagata gtagatttac gccgagacac
     9301 cattactctt cccactttcg agatttttct tcccagtgga acaatggaca atctggtcgc
     9361 tctgcttacc catactcaaa gaggcaatga gataatctta gaagagaatg cccatatttt
     9421 ttacttccga aaccggtggg ttagctcagg tggcaggttt gatggtcaaa actttgcggg
     9481 gtgaaggtgg agtgccagaa ccgcaggata ttgagagaac tattcgacca gaaaatattc
     9541 actatcccag gaccgccctc attgatttag aaaatactca ttatcgctat ggaggcatag
     9601 tagttcccct tgctaaattt gagaaaataa gagaagtggc agatagatat aatttactta
     9661 tctatcttga tggggcaagg cttttcaata ctgcaattta tctgggagta gaagttaaag
     9721 aattgctcgc tattgtgatt cagtcatgat ttctctttcc aaaggtttag tagcccggta
     9781 ggttcgttgc tcgatgggtc ttgcgatttt ataaaagaag ccaagagata ccgtaaaatg
     9841 ttgggaggag ggatgaggca aactggctgg ttgtgcgccg gtggtattgt tgctctttct
     9901 ggagatagca tttctcgcct gcaggaagac catgataatg ctcaatttct ggcagaaggc
     9961 ttgcagtctc tcccggggat aaaaataaat cgacgtcagg tgcaaaccaa ctttgtttta
    10021 gctgatttaa gcgagagtgg cttaaacgct acagtttttc tggaaagaat taaagaaaaa
    10081 gggatcttag ctactaaagc tggagagttt atgattcgtt ttgtcacttc ccgggaggta
    10141 aaaagagaag atattgccct tgcccttcaa gctgtcaaag aagctgggca agtcgtttaa
    10201 tgtctgattg aatctcagcg taaaaaacat aaataaatcc tgaagggtat ctcaaaacga
    10261 atgaagaaaa agacacccct cttttatctt tctatttttc aagttgagat agaaattgga
    10321 gacgagtaag agcaagagtt tgcttaattt tcaattagat agtataattg atttgcttta
    10381 gaacaatagt ttgacaaggg ggaaatagag tactttagcg tggaggagag aaaatctttt
    10441 gaggaaaggg aattatttca gattcaagaa aaagaagtaa aaccgcttgc ctctcgaatg
    10501 cggcctcgca gtttagatga agtagtaggt caacctcatc taaccggaaa agaggggatt
    10561 ttgagaaaga tagtggaaac tggttttttg ccctctctca tattctgggg gccaccgggg
    10621 agcggtaaaa cttctgttgc ttatcttgtg gctaaagctt gcaaatatca gtttatttct
    10681 atcagcgcag ttacttcggg agtgaaggaa ataagagagg cagtaaagga agcagaaaac
    10741 gacctgaaat ttcatcgtaa aaaaaccgtt ttctttattg atgaaattca tcgttttcat
    10801 aaagggcaac aaagtttgct tcttccccat gtagaagagg gaatagttac tttgattggt
    10861 tctaccactg aaaacccttc ttttgaaatt attgccccct tactttctcg ctctcaagta
    10921 ctggtttttc agaaaatccc ggtagaagat ttagtttatt tgttgaaaaa aactttagaa
    10981 gatgagcgag gattaaaaga cgagaaaata aagatagagg aagatgctct tttcttcatg
    11041 gctcaacttg ctgatggaga tgctcgacag gctttgaacc ttttggaggt tgcttctcgt
    11101 attgctcaac aagaaaataa gggggaaata ggggttaagt ttttgcagga tacttttcat
    11161 aaaacttctt atttgtacga taaatcggga gaggagcatt ttaatcttct ttctgcttac
    11221 cataaaagcc tgcgaggtag tgatcctgat gctgctattt actggttagt tcgcatgctg
    11281 gagggaggag aagaccctca tgctattttg cgccgcctgg tagcctgtgc ttcagaagat
    11341 gtgggtaatg ctgaccctca tgctttgctg atagctgttg ctgctcaaga agcctacgat
    11401 tttctgggag aaccggaagg aagactgtct ctggctcaag ccactatata tatagccagt
    11461 gctcctaaaa gtaatgcttc ttatcaagcg ttaaatcagg cgctggcgga tgtgagagaa
    11521 tatggcgcac ttcctgttcc cttgcatttg cgtaatgctc ctactggcat gatgaaggaa
    11581 atgggatttg gcaaggaata tcgatatcct catgacttta atggagcatt tatagaacag
    11641 gaatatctgc ctcctcagct acagggtaaa acctattttc atccccatga tagaggatat
    11701 gaaaagacaa taggagagag attacgcttg tggtggaaaa accggaaata atgaaggcca
    11761 caggcaaata cgcttaaaat gtaaatagcg ttagtgtatt atagttttaa ttacaagatt
    11821 aattgtcagt gtctttgctt ctatgttttg tttttaaatc ttttatttaa tgtcataggc
    11881 tgctatttgc aactcggagt tatttgagtt gaagaaataa ggttttagag gtaaaatact
    11941 ttaggaggtg aagacttatg ccagaaaaga aagaaactta tcggtgcaat gcgtgtggta
    12001 acgtagtaga ggtggaagaa agcgaaaaag aagatttgac ttgctgcggt gagcccatgg
    12061 ttaaagtaga ggaagaaaaa cctaagggtg gcggttgctg cagaaagtaa agtagtgctg
    12121 tttagctaac ttatagtatg gtaataacgc tgggatgcct tctcccagcg ttattatttt
    12181 agtttctcat atttttatag tatttcccaa aacatgtttt aattgttatt cctcatggag
    12241 aagaaaaaga gagcttttca ccttgtgtaa cttttaaggt tctttcaaaa gtgggattgc
    12301 cacaaacatt ctagggcaaa aaataaaaat tgaaagctaa aaggaaagag tccgagggac
    12361 gaaattacct gtggtaatgc ccggggaggg atgaggagaa tacgagggaa agaaacatta
    12421 tcatggattt tgcagttaac tataatcttg catcgtggtc actggggaag ataaatatct
    12481 tccccagtgt tttttatttt caaccttgac ataagttaaa gattttttat tttttcttct
    12541 ctttcttgta acaattggtc gatagctttt atatcttcag tagttaattc ccaatctcca
    12601 gcagggaatg tttcttcaat ctgagagtgg cgacgagttc ccactatagc ggcggtaatc
    12661 tccgggcgtc ttaacaccca ggcgatggcc agttgagcca gagttcttcc atatttttca
    12721 gcaataggtt ttaacttctc tactaattct aagttaatac taaagagggg ctcgttgaac
    12781 tgggggtcac tacgacgatg atcatcagga ggaagatttt gaactctctc tcgggtgaat
    12841 ttgccggtta acaatccttt ttgcatgggg ctgtatacta ttactcccat atttttctcg
    12901 gcacagtaag gtaaaatctc tttttctata tctcgtgtaa tcatgctata agggggctgc
    12961 agagaggcaa tggggtgaat tttttcaatt cttttcattt gttccacatt aaagttggaa
    13021 actccagcgt aacggatttt tccctctttt actaaatcag ccatagtact ccaggcttct
    13081 tcgatatcag gatcagggtc aggccagtga atttgataga ggtctatcac ttctacttcc
    13141 agtcgtctga gactatcttc tacttctctg cgaatgctat ctctttttaa acatccaaaa
    13201 acctctcctt tttcgttcca tacccttccg catttagtgg caataatagg ttttttactc
    13261 atacctttta gggctttagc tattatttct tctgaatgcc ccaatccata aacagcggca
    13321 gtgtctatcc agtttatacc taaatccagg gcgcgatgga tagcagcaat agactcttca
    13381 tcatcttgag ggccccagct gaattgccac cctccacctc ctaatgccca ggaaccaaag
    13441 cctatcaccg tgagttcaag atcagtccat cctaacttcc ttgtgcgcat gtcaggacct
    13501 cctcagatgg ttttttagta ttatacctta taatgtataa gtttgtttaa aagccactga
    13561 aagggtattt ttttattttt tcaaatttca ttgttttttt gctcctggcc aggtggggaa
    13621 ggaacagtag gagagtgctc ttaagtgtgg ccataataac catgctcaat aatggtttgc
    13681 aattagctgg tattcatcag atctggcagg tgggagcttt agggttggct ttgataggca
    13741 gtgttcttct taaccagttt cttattcgcc gaagaaaaat ttgagacgat atgttttatt
    13801 gttggtcatt atcctagttt taggaggaga aagtctcctg gggaaagaaa cttctcaagt
    13861 atttcttctt tccccttctc ttgattattt tcctgctttt ttagaagttc atttaaggag
    13921 attactctct ccttctcagg tgcatttggt taccctccct caggaggaat taccccgggg
    13981 agatgtttct cttttagtgt ttttgggtaa agagcaatat ttttcttcta ttctgctaca
    14041 aattcctcaa aataaaaaac ctttgtttct tttctgggaa agaaaggaaa ttgaaccttc
    14101 ttccgggttt tctttttgta aaataagagt agaaggagaa gagataatag aagctctcta
    14161 ggaagagctg atagaaaagt tttttccgaa tgactggcta aaggagaatt tctcttcttc
    14221 ttttagtaaa gcagaagaaa gtgataaaga tgtactcaaa tagttgattt ctgacaaaga
    14281 aaaatcctta gtaatagcag aaaatactga agcggggaga cagttgattg atgaattctc
    14341 tgaggccgaa tctcctccgc ccattgtggt attggaggct tctccggaaa tgctttttac
    14401 tctacttcag gggaaaataa aagcagtggt ggattttaaa ccctctcaac tggcacaaga
    14461 gatagtgaat ttgatggatt gttttggggc acataagcct attccagaat ttatattatc
    14521 atctatcctc gcctgatact tcctgataat ataaaagaag ctgatggata cgaagtgatt
    14581 tctcgatgtt taacgtgcca gtaattctat ttttatatcc ttaattatt
//
LOCUS       DAOSVE010000037        13975 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_2636_length_13975_cov_67.506250, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000037 DAOSVE010000000
VERSION     DAOSVE010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 13975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13975
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2636_length_13975_cov_67.506250"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="PK016_04545"
     CDS             <1..1022
                     /locus_tag="PK016_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL02372.1"
                     /translation="ALVFYGGWLVMRGEMTVGTLVAFNGYLWSLIWPMRQLGVLLNLT
                     GWAISSGQRIFDLLDTNVSMPVKVNAYKPDKVRGDVRFDNVTFKYSGDVVLEDVSIDA
                     PAGKKMAIMGATGAGKTSIVNLICRFYEPQKGRVLVDNVDVRDWDLKTLRRNVSVVMQ
                     ETFLFSDTIANNIAYGNDNASMDEIVAAAKAACAHDFIMEMPQGYDTIVGERGVGLSG
                     GQKQRIAIARALLINAPILILDDATSNVDMETEHEIQQALKRLMVGRTTFIIAHRISA
                     VKDADEIIVLDRGRIVERGSHDQLLQARGFYYDIFKEQYKDLVAESVTPELVTDDEDE
                     ALWLG"
     gene            1010..2797
                     /locus_tag="PK016_04550"
     CDS             1010..2797
                     /locus_tag="PK016_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL02373.1"
                     /translation="MARVNRFFDDEPLRRPFNKDQFIRLLGYLGPFKKHLLVAFALML
                     VATLAGLAGPYIIKLAIDNYIASSDIKGMIYLTMIFATLTIVTYFMNRKRVTMMTGMG
                     QRMLYNMRMDLFQHIESLSFTFFDSRPAGKIMMRLVNDVNSLNDLLTNGLINVINDFI
                     MLIAIIVIMFSMNVRLTLISFLLLPALVAIMFGLRSVIRERWQMVRQKNSNMNAYLQE
                     SISGMRVTQAFVQEDVNEKIFLDLNNNIKTAHMNAIRVNNAFWPLLNIIGAVGNALVF
                     IVGVGMVTRGEVTVGTLVAFTSYIGRFWMPLSTMSNFYNQLLIAMASAERIFEILDTK
                     PEVTDKPGAIVLPPIKGHVEFDHVYFYYEPDKPVLRDISFVVQPGENIALVGPTGAGK
                     STIINLLARFYDVTGGRVLIDGFDIRDVTLESLRSQLGIVLQDTFLFSGTIMDNIRYG
                     KVDATDEEVIAAAKAVRADDFIREFKNGYFTEVKERGSRLSVGQRQLISFARALLANP
                     RILILDEATSSIDTHTERLVQMGLEQLLKGRTSFIIAHRLSTIRNADRIMVVDEGQIK
                     ESGTHKELMAIGGIYRELYISQFNWLLAG"
     gene            3067..5016
                     /locus_tag="PK016_04555"
     CDS             3067..5016
                     /locus_tag="PK016_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HXL02374.1"
                     /translation="MHKRVISILSVLAFVLLFCGVACAQGEKVVSLPYFDTPGLIPYY
                     WQPQHILAQGTTFEGLFGYAPNPEGMGGVEVVPVIAESYEVSDDYKVWTFKLRKDKKW
                     SNGDPVTAKDFEWSYRYYASPKIPDFPAWAGPLQFFENFWAVKSGALPEDALGVKAID
                     DYTLEIKLYQPKYDLKEWLCVAQGVPLHKGNVEANPENWWKPENIIVNGPYIPKSWTP
                     GEEMVLIKNPNYVGEVGNVDKIVLKFGGPGIQQYQAEELDAVFIQNVAEYKFALADPQ
                     LSQQFHESPMDPFWYGYQLSRGFDPVMDSKELRQALAMAIDRELLCEQVLGGRALPLA
                     SYWPKGTPIGDKLQDIPYDVEKAKELLAEAGYPDGKGLKDLVFYIPSGDLTAEYVAGF
                     IVDQWKKNLGVNCIIENVEVGVYNDAYVWANFTPEAKAGFTTIGGPMNNLEIGALFKN
                     ADHTIWFYDYPAEVRKRLHELYEEYDAWLRKEGGQTEEDMAALVEKKNVLLEGFNKIV
                     ENEPEELWKEEMTRPPLWYEQFDELYEKWKAAGTPSEKTDYWRLAGRVLSEQERFQVE
                     YNGRNPSNKEAYRWRLRTVNSPFDKAVEMAHNALQIMQDQYWMVPVYLFKAQWLQNPK
                     LEGLMVYKFSWGPAFFNFKYLNLAD"
     gene            5078..6040
                     /locus_tag="PK016_04560"
     CDS             5078..6040
                     /locus_tag="PK016_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL02375.1"
                     /translation="MALFKYILRRLLTIFVSVVVVIIITYSLMYIAPGDFFNISSFTA
                     SAGKVSTLTPEQLQILIKGFEEKYGLNVPLWKQIWNYLKDAFRFKFGPSFSNPNEPIE
                     AQIARKFPVTFTLAVLAMLFAVLIGIPLGILAALKKNTWIDYFSMFISMIGSIIPAYL
                     IGLIMVIIFSVYLKVLPTSGWGSLKEMIMPVITVGLGPMAIIARFTRSSLLDTLSQDY
                     IRTAYAKGGTERAVVMRHALKNSLIPVVTVIGPQLAFLFTGTVWIENLFRVPGLGQLF
                     VNAAAQRDYPLLVTSTFILSLSVMLMNLLVDVIYVFLDPRIKYE"
     gene            6067..6939
                     /locus_tag="PK016_04565"
     CDS             6067..6939
                     /locus_tag="PK016_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL02376.1"
                     /translation="MKIVSEQEQKRASYWSSVWRRFKSNRMALVGLIFIIIITVAAIF
                     TPWIAPYNYDETNYAHTFEKPSRQFIWGTDDLGRDMFSRNLYAMRNALLIGFGSQAIV
                     VVVGVIIGAIAGFRGGTVDSVLMRIVDIMYAFPTFLFNVILVTALGRGIPIILLAIGI
                     TGWAGMARLVRGMVMSLKNADFVEAARALGAKEFHIIRKYILPNMFGPIIVSIAFGIP
                     NAIWMESGLALVGMGVRPPMPSWGNMISAGMGYIMAFPHMVIFPAILFALTLLAFTYV
                     GDGLRDAMNPKGEA"
     gene            6942..7919
                     /locus_tag="PK016_04570"
     CDS             6942..7919
                     /locus_tag="PK016_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL02377.1"
                     /translation="MEELLVVNNLKTYFHRYEGIVKAVDGVSYKLNYGETLTIVGESG
                     SGKSVSVLSLLGLIRKNGRITEGEAIFEGRDLFKLNKEELRQIRGKNISIVFQDPMTS
                     LNPIMKTGIQIMEPLIWHRLMSSKKARERAVELLKIVGIPDHHIRAWDYPFEFSGGMR
                     QRVMIAIALACNPKLIIADEPTTALDVTVRAQILNLMQDMKEEFNLSIILITHDIALA
                     MNFSDSIMVMYAGKVAEYAPVAEFIKSPLHPYSQGLINSTLDIEAKEGSIKPIVGAPP
                     NPLNLPPGCRFHPRCGYAEDICRRVEPEYREIGNNHYVACHLAKGGRIR"
     gene            7916..8863
                     /locus_tag="PK016_04575"
     CDS             7916..8863
                     /locus_tag="PK016_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008396473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL02378.1"
                     /translation="MKELLVVKNLKKYFPARGKLLKAVDNVSLSIDSGETLGLVGESG
                     SGKSTLGRSILRLIEPDDGEVIFEGADLRKLKEEELRQKRKFMQIIFQDPLASLNPMM
                     TVGQNIEDPLVIHNIGTREKRRETVVELLDIVGLGKNIIDIFPYELSGGQQQRVGIAR
                     ALALNPKFIVADEPVSALDVSIQAQIVLLLNELKKRFEISYLFISHDLAVVKYLSDRV
                     AVMYLGSIVELSSKEELYKNPLHPYTKALLASIPKMPKNGNPQKRFSALKGEIPSPVD
                     LPPACRFQSRCEYATGACKEQEPVFREVAMGHFVACHLA"
     gene            9046..10011
                     /locus_tag="PK016_04580"
     CDS             9046..10011
                     /locus_tag="PK016_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HXL02379.1"
                     /translation="MLLFVNEACAQGTLPIKRGVNLTGLEAPLEGSWGVMIEDEYFKL
                     VKEAGFDHVRIPIRWNAHSLYESPYTIKDYIFERVDHLVNEAFKNNLYVVINIHHFEE
                     MMKEPEKYRDKFIALWEQIADHYQDYPEGLWFELLNEPCMNLNSDLWNDYLEEAIKVI
                     RKTNPERKIVVGPGDWNNINRLKDLKLPQGDKNIVVTFHYYNPFNFTHQGAEWVSPSP
                     PVGTKWSGSEKEQEAVERELDIAVNWSKDNGDVPLWMGEFGAYNKADLDSRARWTEFV
                     ARAAEKRNIAWAYWDFCSAGFGVYDFFNGKWVLPLLRALIPETKI"
     gene            10021..11016
                     /locus_tag="PK016_04585"
     CDS             10021..11016
                     /locus_tag="PK016_04585"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HXL02380.1"
                     /translation="MEEKDTFPRWRGFNLPNMFSYYDARDFEEEDFKWISEWGFDFVR
                     IPLNYRLWVEEGDIYRIKEDVLKKIDLAVEWGEKYGIHICINFHRAPGYCVNDELPEK
                     FNLWKDEEAANAFCYHWEIFTERYKGISSEVLSFNLVNEPPHPDKEVMTREDHERVIR
                     KTVNHIKRIDPQRLLIIDGISFGNEPLFELSDLAAGQSCRGYLPMGLTHYKASWVGGE
                     NWPEPVWPGAWHYGESWDRKKLEEHYTRWAALKDRGVGVHCGEAGAFKFTPHNVVLAW
                     MKDLLEVLSERDIGFALWNFKGPFGILDSEREDVEYEDWQGHKLDRKMLSLLQDY"
     gene            11161..11400
                     /locus_tag="PK016_04590"
     CDS             11161..11400
                     /locus_tag="PK016_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HXL02381.1"
                     /translation="MVVQNLEQIKEWVASIVGEEVVIEVNKGRKKTITKKGVIEKVYP
                     FFFTMITDVDGSSQRASFNYADILTKTIELEITRS"
     gene            11452..12156
                     /locus_tag="PK016_04595"
     CDS             11452..12156
                     /locus_tag="PK016_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HXL02382.1"
                     /translation="MNKLLDFPYAVDLNEAKKIQEKLKGLLSFNFPYQDEEINLVAGI
                     DVSYNAEKEAFAAVVVLDFPSLRVREVSWGKVFPPFPYIPGFLSFREGPAIERALSLL
                     CFQPQIFFFDGQGIAHPRGVGLATHLGILFGVVSVGVAKSNLVGNFQEPPSKQGSFSW
                     IEYEGKKVGMVLRTKEGVKPVFVSPGNKIDLESAKHWTLLVTTRYRLPEPVRQAHIFS
                     EKIKKGGEEYGRYSFS"
     gene            12134..12451
                     /locus_tag="PK016_04600"
     CDS             12134..12451
                     /locus_tag="PK016_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HXL02383.1"
                     /translation="MDDIPFHELEGEGSTENKVVITVVGKDKIGIIAAITSCLAGYQV
                     NIEDITQKILGEYFTMILVGDISRCPVSLADLRRDLTQKGEEIGVTVYMQHASIFRAM
                     HRI"
     gene            12461..13825
                     /locus_tag="PK016_04605"
     CDS             12461..13825
                     /locus_tag="PK016_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HXL02384.1"
                     /translation="MLFDRSEILETLRMLEIENLDVRTVTLGINLLDCRSGSLSSTIE
                     KVIEKIRHLSLPFHEVVEETFVKYGIPIVNRRIAISPVSFLLDGREKKEEVVALAQRL
                     DDLARELQVDFIGGFTALVPKGFTPGDLSLIDSLPLVLSQTQRICGSVNLASSRSGIN
                     VDAILKMGEIIKETSFLTAQEGGIGCAKLVVFANAPEDNPFMAGAFHGPGEADAALNV
                     GVSGPGVIKEAIRRKPEADLGEIAEVVKRTAFKITRVGELIGKEIAQKLGVSFGIVDL
                     SLAPTPRVGDSVAEILEQMGLEKCGAWGSTLALALLTDACKKGGAMASSSVGGLSGAF
                     IPVSEDAEMTESVKAGALKLEKLEALTSVCSVGLDMVAIPGEVSKETISAIIGDEVAI
                     GVINHKTVGVRIIPVPGKKPGDRVVWGGLLGETVVMEVNPFQSKDFISRGGRVPAPIT
                     SLKN"
BASE COUNT         4180 a         2296 c         3371 g         4128 t
ORIGIN      
        1 tggcattggt gttctatggt ggctggttag taatgagggg ggaaatgact gtaggcacat
       61 tggtagcttt taatggttat ctctggtctc tcatatggcc tatgcgtcag ctgggagttt
      121 tactcaatct gactggatgg gctatatcct cgggtcagcg aatatttgat ctcctggata
      181 caaatgtatc gatgccagtc aaggtaaatg cctataaacc ggataaagta cgaggcgatg
      241 tacgctttga taatgtaact tttaaatata gtggcgatgt ggtacttgaa gatgtaagca
      301 tagatgctcc agcgggcaaa aaaatggcca taatgggtgc taccggagca ggcaaaacct
      361 ctatagtaaa cttgatatgt cgtttctatg aacctcaaaa aggacgagtt ttagtagata
      421 atgtggatgt aagagactgg gacttaaaaa cattacggcg caatgtatca gtggttatgc
      481 aagaaacatt tctgttctct gataccatag ctaataatat agcctatggt aatgataatg
      541 catcaatgga tgaaattgta gcggcagcta aagcagcttg tgctcatgat ttcataatgg
      601 agatgcctca gggttatgac acaatagtgg gtgaacgggg agtgggtttg tcaggaggtc
      661 agaagcaacg catagccatt gcccgggcat tactaataaa tgctcctata ttgatattag
      721 atgatgccac atccaacgtg gatatggaaa ctgaacacga gatacagcag gcactcaaac
      781 gccttatggt gggccgtact acgtttatta tagcccatcg catttcagca gtaaaagatg
      841 ctgatgaaat tattgttctg gatagaggtc gcatagttga gcgtggtagc catgatcagc
      901 tgctccaagc tagaggtttt tattatgata tattcaagga gcaatataag gacctggtgg
      961 cagaaagtgt tactccagag ttagtgactg atgatgagga tgaggcatta tggctagggt
     1021 aaataggttt tttgatgatg aaccactccg taggccattc aataaagatc agtttatccg
     1081 cttgttagga tatctgggcc cttttaaaaa acatctgctc gtggctttcg cacttatgct
     1141 ggtggcgacg ctagccgggt tagctggccc ttatattata aagttagcta tagataatta
     1201 tatagcttcc agtgatataa agggcatgat ttacctcact atgatatttg ctactcttac
     1261 tatagtcact tactttatga accgcaagcg tgttactatg atgactggta tgggtcagcg
     1321 tatgttatat aacatgcgta tggatttatt tcaacacata gagagtctgt catttacctt
     1381 ttttgacagc cgaccagcag gcaaaatcat gatgcggttg gtaaatgatg ttaattctct
     1441 aaacgacctc ctaaccaatg gcctcataaa tgtgattaat gattttataa tgttaatcgc
     1501 tataatcgtt atcatgttta gtatgaatgt acgtcttaca ctgatatcat ttctcctatt
     1561 gccagccctc gtggcaataa tgtttggcct gcgtagtgtc ataagagaac gctggcagat
     1621 ggtgcggcag aaaaactcca atatgaatgc ctatttgcag gaaagcatat caggtatgcg
     1681 tgttactcag gcttttgtgc aggaagatgt taatgaaaag atatttttag atttgaataa
     1741 taatataaaa acagctcata tgaacgctat acgggtcaat aacgctttct ggcctctctt
     1801 aaatatcata ggagcagtgg gtaatgctct ggtattcata gtgggagttg gcatggtaac
     1861 gagaggtgaa gtaactgtgg gtacgctggt agcattcacc agctatatag gtcgattctg
     1921 gatgccgctt tctaccatga gcaacttcta taatcagctt ctcatagcta tggcatcagc
     1981 tgagcgtata tttgaaatac tagatactaa gcctgaggtt actgataaac caggcgcgat
     2041 cgtgcttccg cctataaaag ggcatgtgga attcgatcat gtctattttt actatgaacc
     2101 agataaacca gtgttgaggg atataagttt tgtggttcag cctggagaga acatagcact
     2161 ggtaggacca actggtgcgg gtaagtctac tataataaat cttttagctc gcttctatga
     2221 tgtaacagga ggaagggtgt tgatagacgg attcgatatt cgggatgtaa cactggaatc
     2281 acttcgtagt cagctgggta tagtgttgca ggatactttt cttttttctg gcactataat
     2341 ggataatatt cgctacggta aagtggacgc aacggacgaa gaagtaatag cggcagctaa
     2401 agcggtacgt gctgacgatt ttataaggga atttaaaaat ggctatttta ctgaagttaa
     2461 agaacgaggt tccagattgt cagtgggaca acgtcaactt atatcttttg ctcgtgcact
     2521 tctggctaat ccacgtatac ttatattaga tgaggctact tcaagtatag atacacatac
     2581 tgaacgcctg gtacaaatgg ggttagagca attgctgaaa gggcgcacct cgtttataat
     2641 agcccaccgg ctctctacta tacgcaatgc cgatcgaata atggtagtag atgaagggca
     2701 aattaaggaa tcaggcactc acaaagaatt aatggctata ggtggtatat atcgggagct
     2761 atatatatcg cagtttaact ggttactggc cggatgaatc tttttaagag ttttaccacc
     2821 catattgata tagagccatt gctgtcttga cagattgagg aacaaaagat atacttttta
     2881 aagactaaga aatgaaggga gatggtgaga gaaaaaagag agaatattca taggatggaa
     2941 aaaggttctt tgaacattat ctttttgaaa taagggggtg gtttcttaga ttagattttg
     3001 ttattttatt tactcagtat tttctgcttt agtattcagt ttctgcaaaa gagaggaggt
     3061 ttatcaatgc acaaaagggt tatttcaatt ctatcggttc tggcgtttgt gttgctcttt
     3121 tgtggagtag catgtgcaca gggtgaaaaa gtggtttctt taccttattt tgataccccg
     3181 gggcttattc cctattactg gcaacctcaa cacattttag ctcagggtac cacatttgaa
     3241 gggctttttg gttatgctcc caatcccgag ggcatgggtg gggtggaggt tgttccggtt
     3301 atagctgaga gctatgaggt atcggatgat tataaagtct ggaccttcaa actaagaaaa
     3361 gataaaaagt ggtctaacgg agacccggtt actgcgaaag actttgagtg gagttataga
     3421 tattatgcta gcccaaaaat tcctgatttt ccagcctggg caggtccact tcagttcttt
     3481 gaaaatttct gggcagtaaa aagtggtgct cttcctgagg atgcgcttgg cgtgaaagcc
     3541 attgatgact atactctgga aatcaaactt tatcaaccaa aatatgacct taaagaatgg
     3601 ctttgtgtgg ctcaaggtgt acctcttcac aaaggtaacg ttgaggcaaa tcctgaaaac
     3661 tggtggaagc ccgaaaatat tatagtaaat ggaccttaca ttcctaaatc ctggactcca
     3721 ggagaggaaa tggtgttaat taaaaatcct aattatgttg gtgaggtagg caatgtagac
     3781 aaaattgttc tcaagtttgg aggaccgggg attcaacaat atcaagcaga agaacttgat
     3841 gcggtgttca ttcagaatgt agctgaatat aaatttgctc ttgccgaccc tcaactttct
     3901 caacaatttc atgaatctcc aatggatcca ttctggtatg gttatcagct atctcggggt
     3961 tttgaccctg tgatggatag caaggaattg agacaggctc tggcgatggc aatagataga
     4021 gagcttctct gtgagcaagt acttggtgga agagcccttc ctcttgcctc ttactggcct
     4081 aaagggacac caattggaga taaattgcag gatatcccct atgatgtgga aaaggcaaaa
     4141 gaacttcttg ctgaagctgg atatcctgat ggaaagggat taaaagatct ggtcttctac
     4201 atacccagcg gggatcttac agcagaatat gtggcagggt ttatagtaga tcaatggaag
     4261 aagaatcttg gagtaaattg tataattgaa aacgtagaag tgggagttta taacgatgct
     4321 tatgtatggg cgaatttcac tcctgaagca aaagctggtt ttactacaat tggtggtcct
     4381 atgaataacc tggaaatcgg agcgctgttc aagaacgcag atcatactat ctggttctat
     4441 gattaccctg cagaggtaag aaagagactg cacgagctct atgaagagta tgatgcctgg
     4501 ctcagaaaag aaggtggcca aactgaagaa gatatggcag cattagtgga aaagaaaaat
     4561 gtcctgcttg agggtttcaa caaaatagta gaaaatgagc ccgaagaact atggaaggaa
     4621 gaaatgacta gacctccact ctggtatgag cagtttgatg aactttacga aaaatggaaa
     4681 gctgcaggaa ctccttcgga aaaaaccgat tactggagac tagcaggaag agtattgagt
     4741 gaacaagaaa gattccaggt agaatataat ggaagaaatc caagcaacaa agaagcatac
     4801 cgctggagac tcagaactgt aaatagcccg tttgacaaag cagtagagat ggctcacaat
     4861 gcccttcaga ttatgcagga tcaatactgg atggtgcctg tgtatttatt taaagctcag
     4921 tggttacaga atccaaagct tgaagggttg atggtttata agttctcctg gggccccgcg
     4981 tttttcaact ttaaatactt aaatctcgca gattaaaaag aagcagttta taaaggggga
     5041 agaagacttc ttcccccttt attgaaagga gttaaaaatg gcgcttttta agtatatatt
     5101 gcgaaggctt ctcacaattt ttgtctccgt tgtagtagtt ataattatta cttatagtct
     5161 tatgtatatc gcacctggag atttttttaa cattagctct tttactgcta gtgccgggaa
     5221 agtatccact cttactccag aacaacttca aattttaatt aagggttttg aagaaaaata
     5281 cggcctgaat gttcctctct ggaagcagat atggaattat ttaaaagatg cgttcaggtt
     5341 caagtttggc ccatcttttt ccaatcctaa cgaacccata gaagctcaaa ttgccagaaa
     5401 gtttcctgtt acttttactc ttgctgtttt agccatgctt tttgcagtgc ttatagggat
     5461 tcctttagga attttagctg ctcttaagaa aaatacctgg atcgattatt tttccatgtt
     5521 tatatctatg atcgggagca ttatacctgc ttatcttatt ggtcttatca tggtcatcat
     5581 tttcagcgtt tatcttaagg ttctccccac ttccggatgg ggatctctga aagagatgat
     5641 catgccagta attacagtgg gtcttggtcc tatggctatt attgcacgat tcacgagatc
     5701 gagccttctt gacactctca gccaggatta tatacggact gcctatgcta aaggtggaac
     5761 tgagagagca gtggttatga gacatgcttt gaagaattct ttaattcctg tagttacagt
     5821 tataggtcct caacttgcct ttctctttac tggaactgtc tggattgaga acttgttcag
     5881 agtacctgga ctcggacaat tatttgtcaa tgctgcagct cagagagact accctcttct
     5941 tgtaacctcc acttttattc tttctctctc ggtgatgctt atgaacttgc tggtggatgt
     6001 gatatatgtt tttcttgacc ccagaattaa atacgaataa gccataagag agcgaggttc
     6061 aggaaaatga agatagtatc agaacaagaa caaaaaagag caagttactg gtcatcagtg
     6121 tggagaaggt ttaaaagcaa tagaatggct ttggtggggt tgattttcat tattattatc
     6181 actgttgctg ctatttttac tccctggatt gcaccgtata actatgatga gacaaactac
     6241 gctcatactt ttgagaaacc cagccggcaa tttatatggg ggactgatga tttaggaagg
     6301 gatatgttca gtagaaattt atacgctatg agaaatgctt tactgattgg ctttggttcc
     6361 caggctattg tagtagttgt aggagtgatt ataggggcta ttgcgggatt cagaggtgga
     6421 acggttgata gtgttcttat gagaattgtg gacattatgt atgcgttccc tacttttctt
     6481 ttcaatgtaa tactggttac tgccctgggg agagggattc ctataattct tttagctata
     6541 ggaattacgg gatgggcagg aatggcaagg ttggtgcggg gtatggttat gtcattaaaa
     6601 aatgctgatt ttgtggaagc ggcaagagct ctgggggcaa aagaattcca tattatacga
     6661 aaatatattc ttcctaacat gtttggcccc ataattgtta gtattgcctt tggtatccct
     6721 aacgctatat ggatggaaag tgggttagct cttgtaggta tgggagtaag acccccgatg
     6781 ccaagctggg gcaatatgat aagtgctgga atgggatata taatggcttt tccccatatg
     6841 gttatatttc cagcaatact ttttgctctt actcttctgg ctttcactta tgttggtgat
     6901 ggtttaagag atgcaatgaa tccgaagggt gaggcatagc catggaagaa cttctggtag
     6961 ttaataattt aaaaacttat tttcatcggt atgagggtat agtaaaggca gtagatggtg
     7021 taagttacaa acttaactat ggtgaaaccc tgacgattgt aggtgaaagt ggttcaggta
     7081 aaagcgtgtc tgtgctctct cttttaggac ttataaggaa aaacggaaga attacagaag
     7141 gggaagcaat ttttgaaggc agagatctct ttaaactgaa taaagaagag ttaaggcaga
     7201 taaggggtaa aaatatttcc atcgtcttcc aggatcctat gacgagctta aatccaatta
     7261 tgaagactgg gatacaaatt atggaacctt taatatggca tagacttatg tcaagcaaaa
     7321 aagcgaggga aagagcggtt gaacttttga aaatagtagg gattcctgac caccatataa
     7381 gagcatggga ttaccctttt gagttttctg gagggatgag acagagggtt atgattgcta
     7441 tagctttagc gtgtaaccct aaacttatta ttgctgatga gcccaccaca gctttagatg
     7501 ttactgtaag agcgcaaata ttaaatttga tgcaggatat gaaggaggaa tttaatctga
     7561 gtattatact gataacccat gatattgctt tagctatgaa tttttccgat tctataatgg
     7621 ttatgtatgc agggaaagta gctgaatacg cgcctgttgc cgagttcata aagagtcctt
     7681 tgcaccctta ttctcaggga ttgattaatt ctacccttga tatagaggca aaagagggct
     7741 ctattaaacc aattgtaggg gctcctccta atcctctcaa tttaccgcca ggatgtagat
     7801 ttcatcctcg gtgcgggtac gctgaggata tatgtagaag agtggaacca gagtatagag
     7861 aaataggcaa taatcattat gtagcatgtc atttagcgaa aggggggagg atccgataaa
     7921 agagctcctg gtggtaaaaa atttaaaaaa gtattttcct gccagaggaa aattgttgaa
     7981 agctgtagat aacgtctccc tttctattga ttctggagaa accttaggtc tcgtaggtga
     8041 gagcgggtct ggaaaatcaa ctctgggaag aagtattttg agacttattg aaccagatga
     8101 tggagaggtg atatttgaag gcgccgattt aagaaagtta aaggaagagg aattaagaca
     8161 aaaaagaaaa tttatgcaaa ttatattcca ggatcctctg gcttctctca atcctatgat
     8221 gactgtagga cagaatatag aagatccttt agtaatccac aatataggaa ccagagagaa
     8281 aagaagagaa acagtagtag agcttctgga tatagttggt ttgggaaaga acataataga
     8341 tatttttccc tacgagcttt ctggcggcca acaacagagg gttggaattg caagagcttt
     8401 ggctttaaat cccaaattta ttgttgctga tgaaccagta tcggctctgg acgtttctat
     8461 tcaagcgcaa attgtgttgt tgctcaatga attaaaaaag aggttcgaaa tttcttattt
     8521 atttatttct cacgatttag cggtagtgaa atacctttct gatagagtag cagttatgta
     8581 tctgggatct atcgtagagc tatcttctaa agaagagctg tacaaaaatc ctcttcatcc
     8641 ctatacaaaa gctcttcttg cctctattcc taaaatgcct aaaaacggta atcctcaaaa
     8701 gaggttttcg gctttaaaag gagaaattcc ttctcctgta gaccttccac cagcgtgtag
     8761 atttcagagt agatgcgaat atgcaacagg ggcttgtaag gagcaagaac cggtcttcag
     8821 agaggttgct atgggacatt ttgtggcgtg tcatctggca taaaaggagg aacaaggagg
     8881 ggatttactt ttgattttcg agttttttcg ttagaaaaat aaatggtgga ttctcgatgg
     8941 gggcttgggg aaaccttatg tataggttag gtctcttgag agaaaaaatc ttttagagta
     9001 aggagatgga ccatgaagaa aagaaatctt ttgttgtttt tgtttatgct gctttttgtg
     9061 aatgaggcat gtgctcaagg cactcttcct ataaaaagag gagtaaacct cacggggtta
     9121 gaagcccctc ttgaaggttc ctggggagta atgatagaag atgaatattt taagctggtt
     9181 aaagaagcag gatttgacca tgtgagaata cccataagat ggaatgctca ttctctctat
     9241 gagtctccct ataccatcaa agattatatc tttgaacgag tagaccattt agtgaatgaa
     9301 gcttttaaga ataatcttta tgtggttatc aatattcatc attttgagga aatgatgaaa
     9361 gaacctgaaa aataccggga taaatttata gctctgtggg aacagattgc agaccattac
     9421 caggattatc cagaaggttt gtggtttgag cttctgaatg agccctgtat gaatttgaat
     9481 agcgatttgt ggaatgacta tttagaagaa gctataaagg tcataagaaa gacaaatccc
     9541 gagaggaaaa tagtggtagg tcccggggac tggaacaaca taaacaggct taaggatttg
     9601 aagttgcccc aaggagataa aaatatagta gtaacatttc attattacaa tccttttaat
     9661 tttactcatc agggtgcaga atgggttagc ccatcaccac cagtaggaac aaaatggagt
     9721 ggaagtgaaa aagagcaaga agcagtggag agagaacttg atatagcagt aaactggtca
     9781 aaagataatg gagatgtacc tttatggatg ggagaatttg gagcttacaa taaagcggac
     9841 ctggattccc gagcaagatg gacagagttt gtagcccgag cagcggagaa aagaaacatt
     9901 gcctgggctt actgggattt ttgctcagca gggtttggag tttatgactt ttttaacggc
     9961 aaatgggtac ttcctctttt aagagctctt atccccgaaa ccaaaatata ggaggtaaaa
    10021 atggaggaga aagatacttt tccccgatgg agaggcttta atcttcccaa tatgttttcc
    10081 tattatgatg caagagactt cgaggaggaa gattttaagt ggatttcaga atggggattt
    10141 gattttgtaa gaattccctt aaattataga ctctgggtag aggaaggcga tatatataga
    10201 attaaagaag acgtccttaa aaaaattgat ctggctgtgg agtggggaga gaagtacgga
    10261 attcatatct gtataaactt tcatagagct cctggttatt gtgtaaatga tgaacttcct
    10321 gaaaaattca atctctggaa agatgaagaa gcagcgaatg ctttttgtta tcattgggaa
    10381 atttttacgg aacgatataa aggtatatct tccgaagttt tgagcttcaa cctggtaaac
    10441 gaacctcctc accccgataa agaggttatg actagagaag atcatgaaag agtaatcagg
    10501 aagacggtaa atcatataaa aagaattgac cctcaaaggc ttttgataat agatggaata
    10561 tctttcggga atgaacctct ttttgagctt tcagatttag ctgcagggca gagttgcaga
    10621 ggatatcttc ctatggggct tactcattat aaggcgagct gggtgggagg agagaactgg
    10681 ccggagcctg tttggccggg agcctggcat tatggagaaa gctgggatag gaaaaaattg
    10741 gaggagcact atacccgatg ggcagccctt aaagataggg gggtgggtgt gcattgcgga
    10801 gaagctggag cttttaagtt tactcctcat aatgtagttt tagcctggat gaaagatctc
    10861 cttgaagttc tctcagaaag ggatataggt tttgctttgt ggaatttcaa gggtcccttt
    10921 ggaatattag actcagagag agaagatgta gagtacgaag attggcaggg acataaactg
    10981 gatagaaaaa tgttgagcct tttacaggat tattgattgt ttctagaagt tttagtgagg
    11041 tcacttcggg taatgatgag gattaaaccg acttcgctcg gggatttcgg tgaaggagag
    11101 atttgctttt tgctcttttt tgatgtataa taggagaaaa tttttgagga atgatgttct
    11161 ttggtagttc agaatcttga gcaaatcaaa gaatgggttg cttctattgt gggagaagaa
    11221 gtagttattg aagtcaacaa gggaagaaaa aaaacgataa ctaaaaaagg agtaatagaa
    11281 aaagtttatc ctttcttctt cactatgata acagatgtag atggttcaag tcaaagagct
    11341 tcttttaatt acgctgacat tcttactaag accatagaat tagaaataac ccgttcttga
    11401 aagtttagct tatatcttcc tctttttccc tctttgattt aaaatgtcct tatgaataag
    11461 ttgctcgatt ttccctatgc tgtggattta aacgaagcaa agaagataca ggaaaaactg
    11521 aaaggcttat tatctttcaa cttcccttac caggatgaag agattaattt agtagcagga
    11581 atagatgttt cttacaatgc cgaaaaagaa gcttttgcgg cagtggtagt tttagatttt
    11641 ccttctttga gagtgaggga agtatcctgg ggcaaagttt ttcctccttt tccttatata
    11701 ccaggatttt tgtcctttcg agaaggtccg gctattgaga gggcgttatc tcttttatgt
    11761 tttcaaccgc agattttttt ctttgatgga cagggtatcg ctcaccctcg gggagtaggt
    11821 ttagctaccc acctggggat tttattcgga gtagtttctg tgggggtagc caaaagtaat
    11881 ttagtaggta attttcagga acctccctca aaacaaggct ctttttcctg gatagaatat
    11941 gaggggaaaa aggtgggaat ggtattgcgc actaaagaag gagtaaagcc agtttttgtt
    12001 tctccgggca ataaaattga tttggagagc gcaaagcact ggacattact ggtaaccacc
    12061 aggtatcgtt tgcctgagcc tgttcgccag gctcatattt tcagtgaaaa aattaaaaaa
    12121 ggcggagaag aatatggacg atattccttt tcatgagctg gaaggagaag gtagtacaga
    12181 aaacaaagtg gtcatcaccg tggtgggtaa agataaaata ggcattattg ccgctatcac
    12241 ctcctgtctt gcgggatacc aggtgaacat tgaagatatt actcagaaaa tattaggaga
    12301 gtattttacc atgattttag tgggggatat atcccgctgt ccggtaagtc ttgcagattt
    12361 gcgtcgtgac ctcactcaaa aaggagaaga gatcggggtt acagtttata tgcagcacgc
    12421 cagcattttt cgggctatgc accggatttg aggtgaaaga atgctttttg accgctcaga
    12481 aatattagaa actctacgta tgttagagat agaaaattta gatgttcgga cggtaactct
    12541 gggaatcaat cttctggatt gtcgttctgg gagtctttct tctaccatag aaaaagtgat
    12601 agaaaaaatc cgccatctat cccttccttt tcacgaggta gtagaagaaa cttttgtaaa
    12661 atatggaatc cccatagtga accggcgaat agctatctct ccggtgtctt ttctcttgga
    12721 tggcagagaa aagaaagaag aggttgtagc tctggcacag agattggatg atttagctcg
    12781 ggagctccag gtggatttta tagggggatt tactgccctg gttcccaaag ggtttacccc
    12841 tggagattta tcactcatcg attctcttcc ccttgttcta tctcagactc agagaatttg
    12901 tggctcagta aatttagctt ccagccgcag tggaataaat gtggatgcta ttttaaagat
    12961 gggagagata atcaaagaaa cttctttttt gaccgctcaa gaagggggta taggctgtgc
    13021 caaactggta gtttttgcta atgctccaga agacaatcct ttcatggctg gagctttcca
    13081 cggaccggga gaagcagatg ccgccttgaa tgtaggagta agtgggccgg gggtgattaa
    13141 agaagctatt cgcagaaaac cagaagcaga cttaggagaa atagccgagg tagtaaaaag
    13201 gacggctttt aaaatcacaa gagtaggaga gttaataggt aaagagatag ctcaaaagtt
    13261 gggagtgtct ttcggaattg tagatttatc tttagctccc actcctcggg tgggggatag
    13321 tgtagctgag attttagagc aaatgggctt ggaaaaatgt ggagcctggg gttccacttt
    13381 agctctggct ctcttaactg atgcttgcaa aaaaggagga gctatggctt cttcttcggt
    13441 gggagggtta agcggagctt tcattccagt gagtgaagat gcggagatga ccgagtcagt
    13501 gaaagcaggg gcattgaagc tggaaaaact ggaagctctg accagtgttt gttcggtggg
    13561 gctggatatg gttgctattc ctggtgaggt atctaaagag acaatttctg ccatcatagg
    13621 agatgaagta gctattggag taataaatca caaaactgtg ggggtgcgga tcatacctgt
    13681 tccgggtaag aaacccgggg atagagtagt atggggagga ttattaggag aaacagtggt
    13741 tatggaagtt aatcccttcc agagcaagga cttcatctcc cgagggggga gagttcctgc
    13801 tcctattacc agcctgaaaa attagcaaga tttagtccct aagtttgggg tagatattca
    13861 attgtggtta actacaaaat ccatggtaat gttcttttga atatcatcat tatgagcccc
    13921 gtgcaaatag cgttttaata gagccagcaa tatggagtat tgagagccac taaaa
//
LOCUS       DAOSVE010000038        13879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_2644_length_13879_cov_72.552590, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000038 DAOSVE010000000
VERSION     DAOSVE010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 13879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13879
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2644_length_13879_cov_72.552590"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(515..1420)
                     /gene="ribF"
                     /locus_tag="PK016_04610"
     CDS             complement(515..1420)
                     /gene="ribF"
                     /locus_tag="PK016_04610"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HXL02385.1"
                     /translation="MWQRGIKPAEDSILALGFFDGVHRGHQCLLGEAYRLAQEEKTKL
                     HIFTFYPHPRNFLSSGDVKHILTYREKYCLIQKLFPQSLLQFVHFNRSFREMRAQEFL
                     ALVEEEMRPRRVVVGENFHFGFEAQGDAALLKNYFPFRGIEVTVVPSLQEGGKLISSS
                     RIRWHLENGDLFSANRLLGYSFFLQGRVRRGKGVGRKLGFPTANLYPPSYKLLPASGV
                     YTGYVSGKGIGKRNPSLIYVGHRPTVNFSPRRLVEVFIPDISQELYGERLIIEFQAFL
                     RKEMVFPSLLELKNQIEQDLEALRT"
     gene            complement(1423..2295)
                     /gene="truB"
                     /locus_tag="PK016_04615"
     CDS             complement(1423..2295)
                     /gene="truB"
                     /locus_tag="PK016_04615"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HXL02386.1"
                     /translation="MSGGILNVYKPVGMTSFEITERVGKILGEKAGHTGTLDPFARGV
                     MIICWGKATRLASFFQTLDKTYRARIRFGISTDTYDVTGKIVKVTRGKLSRKEGEELL
                     LKYQGVIKQLVPPFSASKYQGKPRYRYARRGKAVPVREKEVEIYDLELVSWEEGEWPE
                     AEILVRCSSGTYIRSLAHEWGEDSGLGAYLFSLRRGKVGNFSLEQSEEITEREIDRQF
                     LLRKSIPPDQALYWLPSVYLEEKEVNRVKQGRPIFLSSPGEGWVKMYEKDRFLGLGKI
                     EDSGELKSRIILGE"
     gene            complement(2276..3307)
                     /locus_tag="PK016_04620"
     CDS             complement(2276..3307)
                     /locus_tag="PK016_04620"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HXL02387.1"
                     /translation="MEAKKEIIEAFRAHKHFVITSHQNIDGDGLGSMFATFFFLEGEG
                     KEAIMVQDGVVPYFYQFLPGREKVLAPEEFYSLNFSSEVDVVIVLDCSHPDRLGRLKE
                     LCIKASLVVNIDHHPDNSCFGDINWVVPGSPSSTLLVYELIKEAGVKISPDMAVNLLT
                     GFVTDTGGFQFVDMDSHLLRVVEDLVTSGASLAQIMRYAFHFRRLESLKLMGRALDHL
                     FYDEDFRFSVIYLTREDFKSCGAQEEDSEGIVDYGLYIPGAEVSLLFKEVEDRSFRVS
                     LRSQGERDILPIAHHFGGGGHRKAAGFKIKGEFPKVKEEVLRVVREFLSALPSSTEEV
                     KKEIVRRDT"
     gene            complement(3282..3647)
                     /gene="rbfA"
                     /locus_tag="PK016_04625"
     CDS             complement(3282..3647)
                     /gene="rbfA"
                     /locus_tag="PK016_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HXL02388.1"
                     /translation="MKEQRVERVAELIKREVSVILLSRVGDPRLKRFTITRVKLSSDL
                     RQAKVFIYFPEGDKEEKYQALQKATGFIKNELALRLRIKFMPELTFVIDETLDKAFQV
                     VELLNQMERKEDGSEKGDN"
     gene            complement(3683..3964)
                     /locus_tag="PK016_04630"
     CDS             complement(3683..3964)
                     /locus_tag="PK016_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HXL02389.1"
                     /translation="MKIGACQIDIFINESFSLKDKRRVVNSLKERLRNRFNISVIEVS
                     TNAQWRKSSLGISCVGNDEQSVQSLLQQVVQFVEGDDRLEITDLTIEIY"
     gene            complement(3961..5889)
                     /gene="infB"
                     /locus_tag="PK016_04635"
     CDS             complement(3961..5889)
                     /gene="infB"
                     /locus_tag="PK016_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HXL02390.1"
                     /translation="MSKIRVYKVAESLGMPTKEVVDLLKELGVEVKNHMSNVDEELVE
                     LLQEEISYQKKKEEEARKKQEKTLSFDSLPTLREIAKIFNEEVEELILELADLNMIFS
                     PKDFLPPLVVERLARERGWYVKWGKDLKELTQKTLDTKTYPRPPVVTILGHVDHGKTT
                     LLDAIRSSQVAETEFGGITQKIGAYQVEVNGRKITFIDTPGHEAFTAMRARGAQVTDI
                     AVLVVAADDGVMPQTVEAIQHAKAANVPIIVAINKIDKVGANPDRVKQQLSEYGLIPE
                     EWGGETIFVNISALQKKGISELLDLILLQADLLELQARHNGYAIGVVIESRLDRGRGP
                     VATVVVQEGILKVGDYFAAGSSWGRVKSLFNDLGKTVPEAYPSAPVEVVGFSDLPLAG
                     TKLMVVGEEKIAHLIAERRKDKEKEKLLKESKAYSISLEEMLAPEEGKNRELNLILKA
                     DFQGSLEALEKAIASLEGEEVKINILYQGVGNITETDVMLASASRGIVVGFNVKLLSE
                     AAKIAKKEGVEVRLYRVIYEVIDDIEKAIKGLIAPRMVEEVLGRAEVRAIFKIPRVGA
                     IAGSYVTQGKIERGALARVIRREETVKEGARITSLKRFKEDVKEVTQGYECGIGLENF
                     DDFQEGDILEAYVIKEEK"
     gene            complement(5882..6175)
                     /locus_tag="PK016_04640"
     CDS             complement(5882..6175)
                     /locus_tag="PK016_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HXL02391.1"
                     /translation="MVARKIPIRTCIGCRERKEKKALVRLVKKEEGIEIDPTGKKAGR
                     GFYLCPRKECIDKLNLKSLSQALRAEVREEELKKLKEVLGSYVESQGKEVNNV"
     gene            complement(6165..7202)
                     /gene="nusA"
                     /locus_tag="PK016_04645"
     CDS             complement(6165..7202)
                     /gene="nusA"
                     /locus_tag="PK016_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HXL02392.1"
                     /translation="MSKDLFAAIEQIEKTKGIPRETLQEAIEAALLSAYRKSFKSSHK
                     GVSVILDPQDGSIKVFARKTIVDKLKDSTTEILQEEAKRLGIDAQVGEVVEIEVTPQD
                     FGRIAAQTAKQVIVQRIREAERDLVYAEFVEKAGEIITGIISKKEGSNIIVDLGKAEG
                     LLPFSEQVSTENYRPGGRMKLYVIEVKKTSKGSRIVLSRTHPGLVRRLFELEVPEVHD
                     GLVDIKAIAREPGVRSKVVVESRSEKIDPVGSCIGNRGSRVKNITDELRGEKIDIIRW
                     SNDPRVLIAESLSPARVVKVELDEKSGTARVFVPEDQLSLAIGKDGQNARLAARVTNW
                     RIDIKGEKDSG"
     gene            complement(7934..9253)
                     /locus_tag="PK016_04650"
     CDS             complement(7934..9253)
                     /locus_tag="PK016_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012660703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HXL02393.1"
                     /translation="MKESRIILSEREIPTTWYNILPDLPRPLDPPLNPVTKEPAKPED
                     FLPIFPPSLIEQEMGGERFIDIPGEVLDIYSLWRPTPLIRAWRLEKALGTPARIYYKY
                     EGVSPAGSHKPNTAVPQVYYNKKDGTKRLTTETGAGQWGSALAMACQFFGLECTIYMV
                     KVSYEQKPYRRVLMQTWGGEVYPSPSSRTKAGREVLAKDPNSLGSLGIAISEAIEDAV
                     TTPHSKYSLGSVLNHVLLHQTIVGEEAKLQLEKAGEKYPDIIIGCVGGGSNFGGLLFP
                     FMRDKLKGEKDFRAIAVEPVACSSFLRGKYEYDYADVARTTPLLKMYTLGHDFIPPGI
                     HAGGLRYHGMSPILSLLYNSGFLEAQSYQQKEVFESAMLFAGTEGIVVAPESAHAIKS
                     AIEEAKKCTEEGKEKVILFNLSGHGFLDLGAYDLYINQGLPDINHES"
     gene            complement(9313..10110)
                     /locus_tag="PK016_04655"
     CDS             complement(9313..10110)
                     /locus_tag="PK016_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXL02394.1"
                     /translation="MLQLENVNKYFFRYTQDEQWAVRNLNLKISGGQFVTVVGSNGAG
                     KTTLLNLISGNYLPDSGKIMINGEEVTNLPAYRRAPYIGRVFQNTFQGTAPSLTIEEN
                     LSLAYLKGRRKGLRPAINDEAKKFFQEKLADVGLGLEKRLRERVGLLSGGQRQALALV
                     MATFGHPKILLLDEHTANLDPKTGEKIMELTNKLIEENNLTALMVTHNLQSALSFGDR
                     TLMMDEGEIVLDLAGEERKKMTIEELLERFSELRHKRFTEDRALLSS"
     gene            complement(10127..11044)
                     /locus_tag="PK016_04660"
     CDS             complement(10127..11044)
                     /locus_tag="PK016_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL02395.1"
                     /translation="MSNFFLYSLQEGLLFGILALGVFITFRCLDFPDLTVDGSYPLGA
                     AVFAVMMYHGFGVGWAMLAAFLAGALAGFFTGFLHTFSRIPALLSGILTMTCLYSINL
                     RIMGRPNISLSENLGHRTIFVILQDYFPEVPEVYLRLAFLLILVLGIKLLLDFFLGTE
                     IGLSIRATGDNETLVEAQGINPDYMKLIGVSVSNALVAFSGAMFAQHQGFVDVNMGIG
                     MVVSGLASVIVGEVLMRGKTIFIMTLQVVAGSIVYRLATAIALNWGYNIGFKPYDLKL
                     FTGLIVIIILSYPVLQEKFSRRVRTKQNG"
     gene            complement(11096..12109)
                     /locus_tag="PK016_04665"
     CDS             complement(11096..12109)
                     /locus_tag="PK016_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL02396.1"
                     /translation="MKKILLVSLVICFVMIFSICGWGEEKIKIGIVQIIDHPALNNVR
                     DGVIDALLEVYGYIPGENVEYDVQSAQGDMPTANTIAQKFVSEGVDLIVSIATPASQA
                     AINATKEIPIVFSAVTDPVSAGLVSSLDKPGDNVTGVSDMVPVDRQMYLIKFLFPDTK
                     KVGNLYNAGEVNSVVTNDMAKAACAELGMEIMEATVATTADVSMAARSLVGKVDAMYV
                     ATDNTVISALDTVAKVCADDKIPLFLADPTTVSQGALLALGFDYYLHGRQTADLVARV
                     LQGENPGEIPVEFAKKLKLSFNPQTVKMLGLDEEEFTAKINAFIEEMKTQGVEVSLEV
                     LPG"
     misc_binding    complement(12157..12373)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12429..12971
                     /locus_tag="PK016_04670"
     CDS             12429..12971
                     /locus_tag="PK016_04670"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HXL02397.1"
                     /translation="MSRAYFSYLKTILGFSLISWQEDKLRAFILPQKSKEKALSIFQE
                     KARFSFYLHPQEKIPFPSLEETIQNYFDGYPASFPYPFYLDDLPPFTQKVLKTTALIP
                     YREIFTYGQLAEKIGKPRAFRAVGRALGQNPLPLLIPCHRVVARHDWGGYSTYGLRFK
                     ILLLSIESRPYTGSSSMSLV"
     gene            complement(12930..13499)
                     /locus_tag="PK016_04675"
     CDS             complement(12930..13499)
                     /locus_tag="PK016_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HXL02398.1"
                     /translation="MEQKKLVYGGALAGLSLVVSLLIHFPLIPQAPFLLYDPGDIPIL
                     VAGFKFGPGLGLLLTAMVAVLFALITGEGGPWGVLMHFLATGSYVVLAGFIYRRHRTK
                     NGAITSLIVAPLFMAAIMVGANLLVTPLYLGVEREVVKGMLLPAIVPFNLLKGVINGA
                     ITLLVYKRISNFIEEPLYERHRATTRVRS"
     regulatory      complement(13582..13724)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
BASE COUNT         3891 a         3498 c         2447 g         4043 t
ORIGIN      
        1 aaagtgattg tcaacagaaa atggaaaaaa attaagggct gaaacgatta gaaataatgc
       61 gtttgaggca ctactacgag tttctcctcc tgtctgtggc atgatgatct gattttttct
      121 ccctccatca tagtatcccg caaaactggt tataatttct ccccagacag aaaagaaaag
      181 gagatattac cgcgccgggc gagtagaaga ggctcgcagt aagtgtgata aatgtcaaga
      241 ggtgggtagc aaattttcca cctcctgaag agagaatttc tgccaggatg tattgtatct
      301 gaccatggca ttcataatag taagaagctt cctcgtgcaa gcaattatag ctactttagg
      361 gggaaatctt tccttcccgg ttgttttatc ttttacatct tttatttttt cgctttttaa
      421 ggggggcgat agggagattt ttcctccccc tatagttcta aaacactcag gaatgacgag
      481 aaagaagagg gttataggga tgtcccctta tagttcaggt gcgcaaagct tctaaatctt
      541 gctcaatctg gtttttaagc tccagaagtg aggggaaaac catttccttt ctcaagaaag
      601 cctggaattc tatgatcaac ctttccccat aaagctcttg agaaatatca gggatgaaaa
      661 cttccactaa tcggcgagga gaaaaattga ccgtaggccg gtggcccacg taaataagag
      721 aggggttcct tttccctatc cctttcccgg aaacgtaacc agtatacact ccagacgcag
      781 gaagaagctt gtaagaggga ggataaagat tagcagtggg gaatcctaat tttctcccca
      841 ctccttttcc ccgtcgaact cgtccctgta gaaagaaaga atagcctaat aagcgattgg
      901 cagaaaataa atcaccattt tctaaatgcc atctaatgcg ggaactggag atgagttttc
      961 ccccttcttg gagagaagga actactgtta cttctattcc tctaaaagga aaataatttt
     1021 tcagaagtgc agcatcgcct tgagcctcga aaccgaaatg aaaattctcc cccaccacca
     1081 ctctccgggg tctcatttct tcttccacaa gagctaaaaa ttcctgagct ctcatttccc
     1141 gaaagcttct attaaaatgc acaaattgca aaagagattg aggaaataac ttctgtatca
     1201 gacaatattt ctctcgatag gtaaggatat gcttaacatc ccctgaggag agaaaatttc
     1261 gaggatgggg ataaaaagtg aaaatatgca atttagtttt ttcttcctga gccagacgat
     1321 aagcttcacc caacaggcat tgatgaccac gatgaactcc atcgaagaaa cccagagcta
     1381 aaatgctatc ttctgcgggc tttatccctc tctgccacat atttattctc ctaaaattat
     1441 ccgggacttt aattctccgc tatcttctat cttacccagc cccaaaaacc tgtccttttc
     1501 atacatctta acccatcctt ctcccggaga ggaaagaaaa ataggtcttc cttgttttac
     1561 cctgttgacc tctttctcct ctaaataaac cgagggaagc cagtataaag cttggtcggg
     1621 ggggatactt tttcggagaa gaaactgccg atctatttct ctttctgtta tctcttcgct
     1681 ctgctccaga gaaaaattac ctacctttcc tcggcggaga gaaaaaaggt aagcacctaa
     1741 accgctatct tctccccact cgtgagctaa gctacgaatg taagttccgc tggagcagcg
     1801 gaccaaaatt tcagcctccg gccattctcc ttcttcccag gataccagct ccaggtcgta
     1861 aatttctact tctttttccc gcaccgggac cgctttaccc cggcgagcat atcgatagcg
     1921 aggcttgccc tggtatttac tggcagaaaa agggggtacc agctgtttta tcactccttg
     1981 atatttgagg agaagctctt ctccttcttt tcgggacaat ttgcctctgg taacttttac
     2041 aatcttccct gtaacatcat aagtatcagt agatatgcca aaacggattc gagctcgata
     2101 agttttatcc agagtctgga aaaaagaggc aagacgcgta gctttacccc agcaaattat
     2161 cattaccccc cgggcaaaag ggtcaagagt tccggtatgg ccagcctttt ctcccaggat
     2221 ttttcccacc cgctcggtaa tttcaaagga ggtcatcccc accggtttat aaacattaag
     2281 tatcccgcct gacaatctct ttttttacct cttcagtaga tgatgggaga gcactcagga
     2341 attccctcac cactcttaaa acttcttctt taaccttcgg aaactcacct ttaattttaa
     2401 aacccgcggc tttgcgatgt cctccccctc caaaatggtg agcaataggt aaaatatccc
     2461 tttctccctg agaacgtaaa ctcaccctaa aagacctgtc ttccacttcc ttaaaaagga
     2521 gagaaacttc tgcccccggg atataaagcc cataatctac tattccttcg ctatcctcct
     2581 cctgtgcccc acaggattta aaatcttctc gggttagata gattaccgaa aatcgaaaat
     2641 cttcatcata aaaaaggtga tccagtgctc gacccataag ttttaaagat tccagtctcc
     2701 gaaagtggaa agcataacgc attatttggg ccagagatgc acccgaggta accaaatctt
     2761 ccaccacccg taaaaggtga gaatccatat ccacaaactg aaagccacca gtatcagtta
     2821 caaagccggt aaggagatta actgccatat cagggcttat tttcactccc gcttccttta
     2881 ttaattcgta aaccagaagc gtagaagaag gagagccggg aactacccag tttatatctc
     2941 caaaacaact gttgtcgggg tgatggtcta tattcacaac cagtgaggct ttgatacaca
     3001 actctttcaa cctccccagg cgatcagggt gagaacaatc taaaactatt actacatcta
     3061 cctcagaaga aaaattgagg gaatagaatt cctccggagc gagtaccttc tctctcccgg
     3121 gcaaaaactg gtaaaagtaa ggaactactc catcctgaac cataatagct tcttttcctt
     3181 ctccctccaa aaagaaaaag gtggcaaaca tagaccccaa cccgtctcca tcaatatttt
     3241 gatgagaagt aattacaaaa tgcttgtgag cgcggaaagc ttcaattatc tcctttttcg
     3301 cttccatcct ctttcctttc catttgattg agaagctcta ccacctgaaa agctttatcc
     3361 agagtttcat ctatcacaaa agtaagttcg ggcataaatt taattctcag gcgaagagct
     3421 agttcgtttt ttataaaacc ggtagctttt tgcaatgcct ggtatttttc ttctttatcc
     3481 ccttcgggaa agtaaataaa cactttagcc tgccgcaggt ctgaggataa tttcactcga
     3541 gtaatagtaa atcgcttcaa gcgagggtct cccacccggg ataacaaaat aaccgacact
     3601 tctcttttaa tcaactctgc tacccgctct actctttgct ctttcatcag ggttcacctg
     3661 actttccact tattttccaa tctcaataaa tctctatggt caaatcggta atttccaggc
     3721 ggtcgtctcc ttccacaaat tgaaccacct gctggaggag agattgaaca ctttgctcat
     3781 catttcccac acaggagata cctaagctac tcttcctcca ctgagcattg gtagaaactt
     3841 caattaccga aatgttaaac cgatttcgca atctttcttt cagagaatta accactcttc
     3901 ttttgtcttt aagagagaaa ctctcattga taaaaatgtc aatctgacag gctcctattt
     3961 tcatttttct tctttaatga cataagcctc cagtatatcc ccttcctgga aatcatcaaa
     4021 gttttctaaa cctattccac actcatatcc ttgagttact tcctttacat cttctttaaa
     4081 tctcttaaga gacgtaattc ttgctccctc tttcacggtt tcttctcttc ttataactcg
     4141 tgccagagcc ccccgttcta ttttaccctg agttacataa gaaccagcaa tcgctcctac
     4201 tcgaggtatt ttaaatatcg ccctgacttc cgctctaccc agcacttctt ccaccatacg
     4261 aggtgctata agacctttaa tggccttttc tatatcatca atcacttcat aaataactcg
     4321 ataaagtctt acctccactc cttctttctt ggctatttta gctgcctcac tcagcagttt
     4381 aacattaaag cccaccacta ttccccgtga agccgaagcc agcataacat cagtttcggt
     4441 aatattgcct actccttgat acaggatatt aattttaact tcttcgccct ctaaggaagc
     4501 gatggctttc tccagagctt caagagatcc ttgaaaatcg gccttcaaaa ttaaattaag
     4561 ttcccgattt ttcccctctt ccggagccag catctcctct aaagagatag aataagcttt
     4621 agattctttc aatagtttct ctttttcctt gtcttttctc cgctcagcga tgagatgggc
     4681 tattttttct tcccctacca ccattagctt ggtccctgcc agtggtaaat cagaaaagcc
     4741 caccacctca acgggtgcag aaggataagc ctcaggaaca gttttcccca gatcgttgaa
     4801 taacgatttc actcttcccc agctgcttcc cgctgcaaaa tagtctccca ctttcaatat
     4861 cccctcctgc acaactaccg tagcaaccgg gcctctcccc cgatctaagc gagattctat
     4921 aaccactcct atagcataac cattatgtcg agcctgcagc tctagaagat cggcctgtaa
     4981 aaggattaaa tctaagagtt cagaaatccc ttttttctga agagcagaaa tgttcacaaa
     5041 aatagtttcc cctccccact cctcagggat caagccatat tcggaaagtt gttgcttcac
     5101 tctatccggg ttagccccta ctttatctat cttattaatg gctacaatga tgggaacatt
     5161 ggccgctttc gcatgctgaa tagcctccac agtctggggc ataactccat catcggcagc
     5221 taccaccaaa actgcgatgt cggtaacctg tgctcctcga gctcgcatgg cagtgaatgc
     5281 ttcgtggccg ggagtatcaa tgaaagttat ttttctccca tttacctcca cctgataagc
     5341 accaattttc tgagtgatac caccaaattc agtctccgcc acctgagagc tcctgatagc
     5401 atccagaaga gtcgttttac catggtcaac atgacctaaa atggtaacca ccggaggacg
     5461 aggataggtt ttagtgtcca gtgttttctg ggttaactcc ttcaagtctt ttccccactt
     5521 cacataccag cctctttctc tcgctaatct ttccactaca agaggaggta gaaaatcttt
     5581 agggctgaaa atcatgttta aatcagccag ctccaatatt agctcttcta cttcttcatt
     5641 aaatattttt gctatttctc tcaaagtagg cagggaatca aagctcagag ttttttcttg
     5701 cttctttcga gcttcttctt ccttcttttt ttgatagctt atctcttcct gcaataactc
     5761 aaccagctct tcatcaacat tactcatatg gttcttaact tctactccca gctctttaag
     5821 caaatcaacc acttctttgg taggcattcc taaactttct gctactttgt aaactcttat
     5881 tttagacatt gtttacctcc tttccctggg attctacata gctacccaat acctccttca
     5941 gttttttcaa ttcttcttct ctcacttctg ctcgtaaagc ctgagacagg cttttaaggt
     6001 tcaatttatc tatacattcc ttacgagggc agagatagaa tcccctcccg gctttcttac
     6061 ccgtagggtc tatttcaatt ccctcttctt ttttaaccaa tctaaccaaa gccttttttt
     6121 ctttcctttc tcgacaacca atacaagttc taatgggaat cttcctagcc actatccttt
     6181 tctcccttta tgtcaatcct ccagttagtt acccgagcag ccagacgggc attttgtcca
     6241 tctttaccga tggccaaaga cagctggtct tcgggcacaa aaacccgggc agtaccgctt
     6301 ttttcatcta actctacttt taccacccga gcggggctca aggactcggc aatcaaaacc
     6361 cggggatcat tactccacct tataatatct attttctccc ctctaagctc atcagtgatg
     6421 tttttcactc gagagccccg attgccgata caggagccca ccggatctat tttctcactt
     6481 cggctttcta ctaccacttt gcttctcact cccggttccc gggcaatagc ctttatatct
     6541 accagaccat cgtgtacctc cggaacttcg agctcaaaca accttctcac cagaccagga
     6601 tgagtacgag aaagaacgat tcgagaacct ttactggttt tcttcacttc tataacgtag
     6661 agcttcattc ttccacccgg ccggtagttt tcggtggaaa cttgctcact aaaaggaagt
     6721 aaaccttcgg ctttacccag atccacaatt atgttactac cttctttttt agaaattata
     6781 ccggtaataa tttctcctgc cttttccaca aactcagcat aaaccagatc tctttctgct
     6841 tctcttattc tctgcacaat tacctgttta gcagtttgag cagcaatcct tccaaaatcc
     6901 tggggagtca cttctatctc caccacttct cctacctgag catcaattcc taatctttta
     6961 gcttcttcct gcaatatttc cgtggtagag tccttcaatt tatccactat tgttttccga
     7021 gcgaaaactt ttatgctgcc atcctggggg tcgagaatca ccgaaacccc tttatgagaa
     7081 cttttaaagc tcttccggta ggcagaaaga agagcagcct caatagcttc ctggagcgtc
     7141 tctcgaggaa tgcctttagt tttttctatt tgttcaatag cagcaaaaag atccttactc
     7201 atattcccaa ccctgaaaag gttttcaaga cataaagaac ttataaacaa gaaagagtgg
     7261 gagaacccac tctttcttgt ttatcactct tcgactgtta ttattttaac tcaaattatt
     7321 aaatagtgca aatttttgag agtcatggtt agtgtcaact cattgtagga gtatttttat
     7381 ttttccctct attatttgct caaatcttct ctccgtagta gcttcttgcc acttttcctt
     7441 cttctgtatt gcgactctcg caagattggg agtaagaggg aggaaaattc ggggtttccc
     7501 atttttgtca ttcccgagct gcatccggga atccacgttt ttgctttttc tttggttttt
     7561 aaatgctgtg gaccccggct taaagattgc cggggtgacg aacaagaggt gatagaaaca
     7621 ttcagaagtg acaccccctc cgtcatgcct gggcgctttt atcaggcatc tactgaggag
     7681 gatgttttcc ttcctcagta gtttttgttt ctttatattt tttgttcttt tccggggatt
     7741 ataagggggg taaagccccc cttatgttcg gattcctgat aaatacattc aggggtaacg
     7801 aaaaagaagg ggcaatagag gattaccccc ttatagctct cctaatgtca actctccaag
     7861 aaatagaaaa taaagccctc cgttgagagc cgataaaaaa ctctctccgg agggaaaata
     7921 ggatgcttaa cttttacgat tcgtgattta tatcaggaag accctggtta atgtaaaggt
     7981 cataagctcc taagtctaaa aatccgtggc cactcaagtt aaagagaatg actttctctt
     8041 ttccttcttc ggtgcatttt ttagcttctt ctattgctga ttttatagca tgagcgcttt
     8101 ccggagccac cactatccct tcagtaccgg caaagagcat agcagactca aaaacttctt
     8161 tttgctggta agattgagct tctaaaaagc ctgagttata aagtaaactc aaaatgggtg
     8221 acataccatg gtatcttaaa cctccagcat ggattccagg aggaataaaa tcatgtccca
     8281 gggtatacat ttttaaaaga ggagtggtgc gagctacgtc ggcatagtca tattcatatt
     8341 ttccccgcaa aaaggaggaa caagccaccg gctcaactgc aatagctcga aaatccttct
     8401 ctccttttaa cttgtctcgc atgaagggga aaagaaggcc tccaaaattg cttcctccac
     8461 ccacacaacc aatgataata tcagggtatt tttctcctgc tttttctagt tgcagcttag
     8521 cttcttcccc aacgatggtc tgatgcaaaa ggacatgatt aagcacacta cccaaactat
     8581 acttagaatg gggagtagta acggcatctt caatagcctc actgatagct atccccaagc
     8641 ttcctaaaga gttagggtct ttagctaaaa cttctcttcc cgctttggtc cgagaactgg
     8701 gactagggta aacttctcct ccccaggttt gcattaaaac tcgccggtaa ggcttttgtt
     8761 catagcttac cttcaccatg taaatagtgc attccaggcc aaaaaactgg caagccatag
     8821 ccagggccga cccccactgg ccagcaccag tttcagtggt taatctcttt gtcccatctt
     8881 ttttattata ataaacctgg ggcacagcgg tattgggttt atggctcccc gctggactca
     8941 ctccttcata tttataataa atacgagccg gagtcccaag ggctttctcc agacgccaag
     9001 ctctaataag aggagtggga cgccagagag aataaatatc cagcacctca cccggaatgt
     9061 ctataaacct ttctccaccc atttcctgtt caatcaagct gggtgggaag ataggcaaaa
     9121 aatcctccgg tttggctggc tctttagtaa caggattaag aggagggtct aaaggacggg
     9181 gcaaatcagg gagaatatta taccaggtag taggtatttc cctctcgctt aatattattc
     9241 gactctcttt catgggtctt ctctcctttc tagtacttta ctttctaaaa gagtaaagca
     9301 agtttttaat ttttaagaag ataataatgc tcggtcttcg gtaaatcttt tgtgtctcag
     9361 ctcgctgaat ctctccagaa gctcttcaat agtcatcttc ttcctttctt ctcccgcaag
     9421 gtcaagaact atttctccct catccatcat caaggtacgg tctccaaaac tcaaagcact
     9481 ttgtaaatta tgagtaacca tcaaagcagt gaggttattt tcctcaatca acttattggt
     9541 aagctccata attttttctc ccgtcttagg gtctaaatta gcagtgtgct catcgagaag
     9601 aagaatttta ggatgaccaa aagtagccat aaccaaagcc agagcctgtc tttgccctcc
     9661 agagagcaac cccactcgct ctcgcaatct tttttctagg cctaggccta catcggcgag
     9721 tttttcttga aaaaacttct tagcttcatc atttatagcc ggacgcaagc ctttccttct
     9781 acctttcagg taagcaagcg ataggttttc ttcaatagtc agagaaggag cagtgccctg
     9841 aaaggtgttc tggaataccc tccctatata aggagcccgg cgataagcag gaagattggt
     9901 tacctcttcc ccatttatca ttatcttccc gctatcgggt aaataattac cactgatcag
     9961 gttaagaaga gtggtttttc ctgcaccgtt actccctacc acagtcacaa actgtccccc
    10021 tgagattttc aggttgaggt tacgaaccgc ccactgttca tcctgagtgt agcggaaaaa
    10081 atatttattc acattttcca gttgtagcat taccttattt tctctgttat ccattttgtt
    10141 ttgtcctcac cctccgggaa aatttttctt gcaaaacagg gtaagaaaga atgataatca
    10201 ctataagacc ggtaaataat ttcaggtcat aaggtttaaa accaatattg taaccccagt
    10261 taagagctat agcagtagct aaacgataaa ctattgagcc cgctactact tgaagagtca
    10321 taataaaaat agtctttccc cgcatcaata cctctcctac aatcaccgaa gctaaaccag
    10381 aaactaccat accgataccc atatttacat ccacaaatcc ctgatgttga gcaaacatag
    10441 ctcccgaaaa ggctactaga gcattagaaa cgctaactcc gattaatttc atatagtcgg
    10501 ggtttatacc ctgcgcctct actaaagttt cgttgtctcc cgtagcccta atagataagc
    10561 cgatttctgt tcctaaaaaa aagtccagta aaagcttaat gcccagcact aaaatcagta
    10621 aaaacgccag acgaagataa acttctggca cttctggaaa ataatcttgc aaaataacga
    10681 aaatagttcg atgtccaaga ttttccgaga gagaaatatt aggtcttccc ataatccgga
    10741 gatttatgga gtaaagacag gtcatagtca gtatcccgga aagaagagca ggaatacgag
    10801 aaaaagtatg cagaaagccg gtgaaaaatc cagccagagc cccagctaaa aaagctgcta
    10861 acatcgccca ccccacacca aagccgtgat acatcataac agcaaaaacc gccgctccta
    10921 aggggtagct accatctacc gtgaggtcgg gaaaatcaag acaccgaaaa gtgataaaaa
    10981 cccctaaagc taatattcca aataacaaac cttcctgcaa actataaagg aaaaaattgc
    11041 taattgatta tctctccttt ttaggggaga agactttact tctccccctg tgatgtcatc
    11101 ctggtaatac ttctagagaa acctctactc cctgagtttt catctcttct ataaaagcgt
    11161 taatttttgc cgtaaattcc tcttcatcca gacccagcat tttcaccgtc tgcgggttaa
    11221 aggaaagttt caattttttg gcaaactcca ccggtatctc tcccgggttt tctccctgga
    11281 gaacccgggc caccaaatca gctgtttgtc gaccgtggag ataataatca aaacccagag
    11341 ccagaagtgc tccctggctt acagtggtag ggtcagccag aaaaagagga attttatcgt
    11401 ccgcacaaac tttagctacc gtatctaaag cgcttatcac tgtattatca gtagccacat
    11461 acatagcgtc cacttttccc actaaggatc tcgcagccat agatacatca gcagtagtag
    11521 ctactgtcgc ctccataatt tccataccca gctcagcaca ggccgcttta gccatatcat
    11581 tggtaaccac cgagtttact tctccagcat tataaaggtt gcctactttc ttagtatcag
    11641 gaaaaagaaa ctttatgaga tacatctgtc tatccaccgg caccatatcg gagaccccag
    11701 ttacattatc tcctggttta tccaaactac ttaccaaacc agcgctcacc ggatcggtca
    11761 ccgcggaaaa aactatcgga atttctttag tggcgttaat agctgcctga gaagcaggag
    11821 tggcaataga gacaatcaaa tccactcctt cagaaacaaa tttctgagca atggtattag
    11881 cagtaggcat gtctccttgc gccgattgaa cgtcatactc gacattttca cccggaatat
    11941 aaccatacac ttcaagaagg gcatctatta ccccatccct cacgttatta agagcgggat
    12001 gatctattat ctgcactatc ccgattttaa ttttttcttc cccccaacca caaatggaaa
    12061 atatcattac aaagcaaatt actaaactca cgagaagaat tttcttcact taacctacct
    12121 cctttttcaa tctatataaa caaaagcccc tcgccacaca gggacgaggg gcaaagctcg
    12181 cggtaccacc ctttttgggt attcaacccc actcttttcc tttatcgggg aaacggcaaa
    12241 ccttaatcag gaatctcctt ttcggcttgc tcctccagga cccattcata tttgccccca
    12301 ataccgagct tccaccttcc ccggctctct gcaactggta gcaaaaacta ctttttccct
    12361 tcttcggatt attctatttt ctatataatt tagctataat tatactcaga agcgaggaga
    12421 agtaaagagt gtcaagagct tatttttctt atttaaaaac tattctgggt ttttccctca
    12481 tttcctggca agaagataag ctccgggctt ttatcctgcc ccagaaaagc aaagaaaaag
    12541 ctttatctat ctttcaggaa aaagcccggt ttagtttcta tctccacccc caagaaaaaa
    12601 tcccttttcc ttccttagaa gaaacgattc agaattactt tgatggttat cccgcatctt
    12661 ttccttatcc tttttattta gatgaccttc ctcccttcac ccagaaagtt ctaaaaacta
    12721 ccgccctcat cccctatagg gaaatattca cttatggaca actagcagaa aaaataggaa
    12781 aaccccgagc ttttcgtgcc gtaggaagag ctctgggaca aaacccccta ccccttttaa
    12841 ttccctgcca tcgagtagtt gcccgccacg actggggagg ttactctacc tacgggttac
    12901 gatttaaaat cctccttctt tccatagagt caagaccgta cacgggtagt agctcgatgt
    12961 ctctcgtata aaggttcttc tataaagtta ctaatcctct tataaactaa aagagtaata
    13021 gctccattta ttactccctt caaaagattg aaaggaacaa tagcaggaag aagcatccct
    13081 ttaactactt ctctttccac ccccaggtaa agaggagtta ccagcaaatt agctcctacc
    13141 ataattgcag ccataaaaag cggggccact attaaactgg ttatcgcacc attttttgta
    13201 cgatgtcttc gatagataaa gccagccaaa actacatagc taccagtggc taaaaagtgc
    13261 attagcaccc cccagggacc tccttcacca gtgattagag caaatagcac cgctaccata
    13321 gcagtcaaca atagccccag ccccggacca aatttaaaac cggccaccaa aatgggaata
    13381 tcccctgggt cataaagaag aaaaggtgct tgaggaataa ggggaaaatg aattaacaaa
    13441 cttactacta aagacaaacc ggccaatgct ccgccataaa ccagtttttt ttgttccacc
    13501 aaaggtacct cctttttctt ctccagggtc aaaaaagccc tggaaatttg aacttccagg
    13561 gctttcaagt gcttttattt tttacttctt ctcccttccg gactttaccg tcggctccgg
    13621 cttctcacca gatctacccc cagggggctc gcgggctcag ggctttcacc ctttaccgcc
    13681 ggtcaggaat tgggtttaac cctcaccctg ccctgaagaa gtttctttta ccgcacttat
    13741 tttaaaagta agatcttgag ctgtcaagag agcgaagagg cggttggaaa aatgctcata
    13801 gttttagaaa aaacagcttc tttgtatacc aaaaaataca tgtctacctc ttcagatctt
    13861 gaactacccc actgaggga
//
LOCUS       DAOSVE010000039        13843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_2654_length_13843_cov_74.406295, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000039 DAOSVE010000000
VERSION     DAOSVE010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 13843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13843
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2654_length_13843_cov_74.406295"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PK016_04680"
     CDS             <1..208
                     /locus_tag="PK016_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02399.1"
                     /translation="SVVFVFSVFNFLLLGIIKRHSPFIVRDYKEVYPTEEDVFSPSVV
                     FILYVFCFSKGVIGGSVSPYSSDS"
     gene            complement(439..816)
                     /locus_tag="PK016_04685"
     CDS             complement(439..816)
                     /locus_tag="PK016_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017294353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HXL02400.1"
                     /translation="MKFKVDENLPIEVAQLFKDAGFEADTVYDEGRMGDSDSSIFNVC
                     RQKGMILVTLDLGFSDIRSYPPHTHPGIIILKLKSQSKTKIVQNVKKIISVIGEEQIQ
                     GCLWIVEEKRIKIRGLTESSEKN"
     gene            complement(826..1050)
                     /locus_tag="PK016_04690"
     CDS             complement(826..1050)
                     /locus_tag="PK016_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HXL02401.1"
                     /translation="MAYLDRISVDPDICHGMPCIVGTRIPVSVILDNLSEGVSQEEIL
                     KSYPSLHPEDISAALAYAALLARECHIIIE"
     gene            complement(1731..2018)
                     /locus_tag="PK016_04695"
     CDS             complement(1731..2018)
                     /locus_tag="PK016_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bh protein"
                     /protein_id="HXL02402.1"
                     /translation="MRCLSCQRETEHRLIYQGEMVKSIVCQECGLEISFDKDRLLALY
                     GEDLVKRVLTKPYRVTEEYREDLVKFFSSIPFRIITKPYRILKEIDSLHDD"
     gene            complement(2061..3356)
                     /locus_tag="PK016_04700"
     CDS             complement(2061..3356)
                     /locus_tag="PK016_04700"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HXL02403.1"
                     /translation="MEDKKMVKIGVAGFGTVGVGTIKILKERKEEIEKELEVNIQIVK
                     IADKDWERERPVMVSPEIRAYDPWEVVKDPEIDIVVEAMGGVSPAFELVSFALGQGKT
                     VVTPNKELIAKKGEELLKIAEKSNSELFFEGAVGGGIPIIHSLREQLAGDDIEEVIGI
                     VNGTTNFILTEMSLRQTSYIEALKEAQSKGYAEPVPVMDVEGFDATYKIAILASLAFH
                     SKVEVEKVYREGITLLIPEDIEFAKELGFAVKLLAIAKKLNGMMELRVHPVLLPLDHP
                     LSEVYGVENAIYVKSKTRRLTFRGPGAGGEATGSALVGDIIEAVRNWKWQCRGRVGSS
                     CLQDMEVKSITELVTQYCVRVLALDVPGVLGKIATQFGEAQVSLSAVKQPVSTPGELT
                     NIYFLTHKAKEENLQKSLEQIRKLEVVREAYAIRVEEGS"
     gene            complement(3367..4107)
                     /locus_tag="PK016_04705"
     CDS             complement(3367..4107)
                     /locus_tag="PK016_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HXL02404.1"
                     /translation="MSDWIIKVSNLNKYFGSHHVLKDINFQVKFGEVVSIIGASGSGK
                     STLLRCLNGLEPVQSGEIIVDGVKINGEKVDLILIRQSIGMIFQSFNLFPHMSVLDNI
                     ILAPHRVKKIPRREAEEIALELLSKVGLAEKAHSYPYQLSGGQKQRVAIARALAMNPK
                     VMMFDEPTSALDPETVNDVLDVMKELAEEGMTMIVVTHEMGFAREVSQRVIHIDEGRI
                     VEEGTPEEIFYHPQNPRTRAFLSKVINV"
     gene            complement(4100..4756)
                     /locus_tag="PK016_04710"
     CDS             complement(4100..4756)
                     /locus_tag="PK016_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HXL02405.1"
                     /translation="MQFDFLALYKALPALLVGALVTLRVTALSIAVGLIIGLGAGLAR
                     VWPNSFLRNVSSAYIELIRGTPLLVQIFLVYFGLPALGLNLDPFVAGVLAMGINSGAY
                     VGEIVRGGIESIAQGQMEAALSLGMSWWQSMYYVILPQALFRILPSLGNEFIALLKDS
                     SLVSTIAIAELTRTGQIIITRTFKSFEIWVGVALFYFIMTYAISRAVKYSEKRMRYSE
                     "
     gene            complement(4779..5549)
                     /locus_tag="PK016_04715"
     CDS             complement(4779..5549)
                     /locus_tag="PK016_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HXL02406.1"
                     /translation="MKRNYLFWLGILVFSLVFAGIANASTLDDIKARGKIIIGTDATY
                     PPMEFYDENGEIVGFDIDLGRAIAEELGVEAVFIDTSWDGIFPALDAKKFDVIMSATS
                     ITEERLKSKAMSDPYYVTSQAIAVRKDNDSIKGPEDLVGKVIAVQIGTTGDLAVSEIE
                     GVKEIKRFDTTDKAYMEVMNGRADAVVNDLSEVAYRMKFLPDMKIVATFKEGEENYGV
                     TLRKEDTDLLEAINQALKNIKESGKYDQIYQKWFGEAK"
     gene            complement(5881..7242)
                     /gene="radA"
                     /locus_tag="PK016_04720"
     CDS             complement(5881..7242)
                     /gene="radA"
                     /locus_tag="PK016_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HXL02407.1"
                     /translation="MNYRCSNCGYSTPRWLGKCPQCGEWNSFLPVSREATKKERSSSY
                     SSPLSLSEIKEKKIQLGERFSTSLREVDRVLGGGVVEGSLILLSGDPGIGKSTLLLKI
                     AEGIARQGKKVLYVSSEESLYQVYLRVERLGLDYNPPLFLLSANSFEEIREGISSLQP
                     QVVIVDSIQNLGREDLEFLPGSVATLRDLSSRFMEIAKKENIVLFLVGHVTKEGTIAG
                     PKVLEHLVDVVLYLEGDANHPLRLLRSMKNRFGSTQEVGILEMQEKGLVEVVDPSHYF
                     MKEGKLSGIGTARTVWAEGKRAMVVEIQSLVHPSYFDFPRRVSLGMDLNRLHLLLAVI
                     EKNTRLRFSRQDVYLSMVGGIKTSETALDLPVCFSLVSSRLNREIDKETIYLGEVGLS
                     GEIRPVPLLEQRINESARMGIKRVVISSYHSRDFLSEHRGEVALVGYSNIREALEKEG
                     LIS"
     gene            complement(7252..9717)
                     /locus_tag="PK016_04725"
     CDS             complement(7252..9717)
                     /locus_tag="PK016_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HXL02408.1"
                     /translation="MFERYTERARKVIILAQDEAVRLKHNHIGTEHLLLGLLREREGV
                     AAKILDSFDITMDLVRAELENFVDQREYQSSREVAFTPRAKRVLELALDETRRLGHNY
                     VGTEHILLGILREGEGVGAQILQRLGLDLETVRAKLYEILSENPDEAGGLPEANNPSP
                     ESRTPVLDEFSRDLTRLAIENKLDPVIGREKEIERVIQILSRRTKNNPVLLGEPGVGK
                     TAIVEGLAQKIVKGDIPEILKGKRVISLDLAAIVAGTKYRGEFEKRLKKIVSEIVQSK
                     EVVLFIDEIHTLVGAGAAEGAIDAANILKPALARGELQTIGATTIMEYRKYIEKDSAL
                     ERRFQPVYVDEPSVDVSIEILRGLKERYEEHHRVKISDQAVEAAVKLSQRYIADRYLP
                     DKAIDVMDEASSRVKLQAMVLPESLKELEKASEEIKKLKEKAVKNQDFEEAAHYRDEE
                     EKLKKKYRDKKESWLKEVDNSRPSVGEEDIATVVSSWTGIPVSRLDAEEKEKLVNMEK
                     EIHERIVGQEEAVEAVCRAIRRSRAGLKDPNRPISSFIFLGPSGVGKTELAKALAEFL
                     FGKEDNLISLDMSEYMEKFTASRLIGSPPGYVGYEEGGQLTEKVRRKPYSVVLFDEIE
                     KAHPDVFNILLQVMEEGRLTDAQGRAVDFKNTIIIMTSNLGARLISSQSGIGFSERSE
                     KEVMSYQEIKQKVTEEVKKVFTPEFLNRVDEIIVFRPLNQKEIEAIVDLMMKETQKRM
                     KEHNMEIDLTPETRFLIAQEGYDPNFGARPLRRAIQKLIEDPLSDLMLKGVFKEGDRI
                     LVGVEGGKINFTKLLPLELSLKS"
     gene            complement(9710..10711)
                     /locus_tag="PK016_04730"
     CDS             complement(9710..10711)
                     /locus_tag="PK016_04730"
                     /inference="COORDINATES: protein motif:HMM:NF012443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02409.1"
                     /translation="MNRRNGLLNNFNRVALVLSRNFAHVPFSSSIYFSPLWEEEVKKR
                     AKEVYSFLARRGFSWVELSSSSKELELLSWVFPQLLSDSSGKILIHRGNLGAIINYQD
                     HLSLFVEGEYQSLEPYWKKLNKWDDFWEGKLEYAYSEELGYLTSSPSRLGTGFKIYMR
                     LHLPALSFIYGEEKVFQWFKDSDKFSVEVYGESNQALAHLFVFSNLFTLGVKEIEILK
                     TIQDFGRGVWEWEKQARASLVKSSSRQDIFRQMMECLWKELIEENEEQNEKKFLDFVS
                     FWLLAQSEGILPESWGFQFMKTIDLWGKVMSGRLLRGQSLKVLGFLGIELGRIWNDV"
     gene            complement(10738..11226)
                     /locus_tag="PK016_04735"
     CDS             complement(10738..11226)
                     /locus_tag="PK016_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02410.1"
                     /translation="MKCDICGWREAKVVVYLIGGEREVKNKLFLCPVCSQQVSISRVV
                     SSGSRENSPLARVFPLEEKEGERCSFCQWRWEDFLNRGVVGCPHCYNFYYQEIESWLS
                     ENQMGLRHRGKIPINWRRQKKIQNAIFRLGRDLKNAIEQENYEKAGHLKKVMEKLRSQ
                     LK"
     gene            complement(11227..11709)
                     /locus_tag="PK016_04740"
     CDS             complement(11227..11709)
                     /locus_tag="PK016_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HXL02411.1"
                     /translation="MYSLCDDIEQHIIKLIEGSPQQMITIQRGRLADEFDCAPSQINY
                     VLATRFNVFRGFIVESRRGGSGYVRIRRVPIDRLEPVVVYLDDNEESLKESDVEDLVS
                     WLEREEFVTPREGALIRAATIDALRKLAGLDFSSVADRNRLRSRIFREILLQVLRWVE
                     "
     gene            complement(11775..13268)
                     /gene="lysS"
                     /locus_tag="PK016_04745"
     CDS             complement(11775..13268)
                     /gene="lysS"
                     /locus_tag="PK016_04745"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HXL02412.1"
                     /translation="MSKELMDEEATRYEENTEYKREKIQILKDLGIDPYNGSFYKTHD
                     IEEIINQADNLVNKDVKVKIAGRIMALRRHGKAIFADLQDRTARIQIYFRKDTIGEDS
                     FTLLREIEVGDVIGVEGIIFRTHAGELTVLVEKFTLLSKCLRPLPEKWHGLKEVEVRY
                     RKRYLDLIMNPNVRRIFYLRSQLLKELRIFLDQKGFWEVETPMMQVIPGGALARPFKT
                     FHNALGMELYLRIAPELFLKRLIIGGLEKVYEINRSFRNEGISTRHNPEFTMLELYQA
                     YADYKVMMELSEEMLSQVVKKITGSYKIEYQSMALDFTPPWRRISLPQTIQELVGVDI
                     VEDSLETIRAKAKERGLIYEDNWDRGKFVNEFLEKFVQPQLINPTFVFDYPVEISPLA
                     KAKKDNPQLAERFELFIGKEEVGNAYSELNDPFEQKERFVDQLRKREKGDLEAHLIDE
                     DYIEALEYGMPPTGGLGIGIDRLMMLITNSPSIREVILFPLLRSSVD"
     gene            complement(13261..13758)
                     /gene="greA"
                     /locus_tag="PK016_04750"
     CDS             complement(13261..13758)
                     /gene="greA"
                     /locus_tag="PK016_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HXL02413.1"
                     /translation="MEKKRDDGRIVLTKEGYQKLEEELKYLKTVKRKEIADKISHALS
                     FGDLSENAEYEEAKNEQAFIEGKILSIEEKLRKAEIVEDERGNSDRVTLGSRVILENL
                     DQNKKMEYFIVDSVEADPSARKISFESPLAQALLNKRKGEEVEIKVPAGVAHYRIIEV
                     KGKDE"
BASE COUNT         3987 a         3369 c         2264 g         4223 t
ORIGIN      
        1 ctcagtagtc tttgtctttt cagtttttaa ttttttgctt ttggggatta taaagaggca
       61 cagccccttt atagttcggg attataaaga ggtttacccc actgaggaag acgttttttc
      121 tccctcagta gtctttattc tttatgtttt ttgtttttcc aagggggtaa tagggggatc
      181 ggtttccccc tatagttcag attcctgata gaaccactca ggaatgacgg ctatgagagt
      241 tattgtggtg gcaaaagaaa ggggttggca agggggaatt ctttccccct tgcactggaa
      301 gagacgcttt ttctcttcca gtagtttttt atgcttactc tttttgcttt ttatgttttt
      361 tgctttcggg gtgatagggg gtctttttcc cctatagtca gggttatgag ggtcaagacc
      421 ttctggaaaa caatacaatc aattcttttc acttgattct gttaggcctc tgattttaat
      481 tcttttctcc tctactatcc agagacatcc ctggatttgt tcttcaccaa tgacagaaat
      541 gatcttttta acgttctgga ctattttagt tttactctga ctttttaatt taagaataat
      601 aatcccgggg tgagtatgag gcggataact acgaatgtca gaaaaaccca ggtctaaggt
      661 tacaagtatc atccccttct gtctacagac attaaaaatt gatgagtcgg aatctcccat
      721 cctcccttca tcataaacag tgtcagcttc aaatcctgca tccttaaata attgagcaac
      781 ttctatagga agattctcat caactttaaa cttcatttaa ctaccctatt caataataat
      841 atggcattct ctggctaaaa gtgcagcata agccaatgca gcactaatat cttcgggatg
      901 aagagaagga tagcttttca aaatctcttc ctgactaact ccttcagaga ggttatccag
      961 tatcactgag acaggaattc tggtgcccac tatacatggc ataccatgac aaatatcagg
     1021 atctacagat attctgtcta aataagccat ttacagtcac ctcaacatat attttaccct
     1081 tattttcagg ctttaactta acaacttgct attattatag cgtagttaga taagcgatta
     1141 ttcctcattg ttgtctcttt taacagaact atgaagggca ttgccctcta taaaaagcag
     1201 actacagcgt aaacaaaaaa ccgaaagtat gaaaaacaaa atgctactga ggaaaaaata
     1261 tctctcagta aaagtataaa ttcccaccat cttcttcatc tgtcatgcct gagcgcttta
     1321 atcaggaatc cagaagtttt tcaatactgt agaccccggc tcgaaagtcg ccggggtgac
     1381 ggacttgaga aaactcatcc tcaccctaac cctctcccgt cgagggagag ggaaagaaaa
     1441 aaagagtaga ggaacactct cgtttgtcat tcccgagatt ttttatcggg aatccaaagg
     1501 tctttactga ggggaagaaa actcccctca gtggttttta cattttcagc ctttcagggg
     1561 ttttataaag gggatttatc cccctcatag ctctgcggca caagctgtca ggcgaggctc
     1621 gcaacaacgg aaagataaga aaaaacagtc aaataagtac gccatagcaa tactctggta
     1681 ctgaaagcag aggaaactat aaaagtaatt tcccctattg ctctgttttt tcaatcatcg
     1741 tggagagagt ctatttcctt caatatccga tagggtttag tgatgatgcg aaagggaata
     1801 ctgctaaaga atttaactaa atcttctcta tattcttcag taactcgata aggcttggta
     1861 agaactcttt ttaccaggtc ttctccataa agagctaaaa gtctatcttt atcgaagctg
     1921 atctccaggc cgcattcctg gcaaactatg gattttacca tctctccctg ataaatcaaa
     1981 cgatgctcag tttctcgctg gcagctcaaa cagcgcaaaa ctgtggtaac ttcttcctta
     2041 tccagaggct tttcctccct tcaacttcct tcttccaccc ggatagcata ggcttctctc
     2101 accacttcga gcttcctgat ttgttccagc gatttctgta aattttcttc ttttgcttta
     2161 tgggtgagaa aataaatatt agtaagctcg cccggggtgc taaccggttg tttcactgcc
     2221 gaaaggctaa cctgagcctc cccaaattgg gtagcaatct tacccagcac tcctggcaca
     2281 tctaatgcca gaacccgcac acaatattga gtaaccagct ccgtgatgga ttttacttcc
     2341 atatcttgca aacaactact acccactctt cctcgacact gccacttcca gttacgaaca
     2401 gcctctatta tatctcccac gagagcacta ccggtagcct ctccccctgc tcccgggcct
     2461 cggaaagtca gccggcgagt tttcgactta acataaatag cattttccac cccgtatacc
     2521 tcagatagag gatgatcgag gggtaagagc acaggatgaa cccgaagttc catcataccg
     2581 tttaactttt tagcaatagc tagaagtttt actgcaaacc ccaattcttt agcaaactcg
     2641 atatcttcgg gaatcaaaag agtgattccc tctcgataaa ctttttctac ctccacttta
     2701 gaatgaaaag ccagagaggc aagaatagct attttgtaag tagcatcaaa accttctaca
     2761 tccataaccg ggactggctc agcataacct ttactctgcg cttcttttag cgcttcaata
     2821 taggaggtct ggcggagaga catctcggta agaatgaaat tggtagtacc attcactatt
     2881 ccgataacct cctctatatc atcacccgcc agctgttccc tgagagagtg gatgataggt
     2941 attcctcctc ccaccgctcc ctcaaaaaaa agctcactat tcgatttttc agcaattttt
     3001 aatagctctt ctcccttttt ggcaataagc tccttattag gggtaaccac cgttttaccc
     3061 tgtccgagag caaaagagac aagttcaaaa gcaggagaaa ctcctcccat cgcctctacc
     3121 actatatcaa tttccgggtc ttttactact tcccaggggt cataagctct aatttctgga
     3181 gacaccatta ctggcctttc tctttcccag tctttatctg caattttaac tatctggata
     3241 tttacttcca gctccttctc tatttcctct tttctctctt tcagtatttt tatcgttcct
     3301 acccccactg ttccaaaacc tgccacgcca atttttacca tttttttatc ttccatttct
     3361 tcaccttcaa acatttatta ctttactcaa aaaagcccgg gtgcgaggat tctgaggatg
     3421 ataaaaaatt tcctccggcg ttccctcttc tactattcta ccctcatcga tatgaattac
     3481 tcgctgggaa acttctcgag caaaccccat ctcatgagta actacaatca tagtcatccc
     3541 ttcttcagct aattctttca tcacgtctaa aacgtcgttt actgtttccg ggtccagagc
     3601 tgaagtcggt tcatcaaaca tcatcacttt aggattcatt gctaaggctc gagcgatagc
     3661 cactctttgc ttttgccctc ccgaaagctg ataagggtaa gaatgagcct tctctgccag
     3721 gcctacttta ctcaaaagct ctaaggctat ttcctctgct tcccggcgag gaatcttttt
     3781 cactctgtgg ggagcgagga taatattatc cagaacactc atatgaggaa agaggttaaa
     3841 actttgaaaa atcatcccga tggattgcct tatcagtatc aaatctactt tctccccgtt
     3901 tattttcact ccatctacta ttatttctcc actctgaaca ggttccagac cattgagaca
     3961 gcgaagcaaa gtgcttttac ccgaaccgga ggcaccgatt atcgatacca cttctccgaa
     4021 cttaacttga aaattgatat cttttaaaac atggtgagaa ccaaagtatt tattgaggtt
     4081 agaaactttt attatccagt cactcactgt agcgcatcct tttttctgaa tattttaccg
     4141 ctcgagaaat ggcataggtc ataataaaat aaaagagagc cactcctacc cagatttcaa
     4201 aagatttaaa ggtgcgggta atgatgattt gcccggtacg agttagctca gcaatggcaa
     4261 tagtggaaac tagcgaagag tctttcaaaa gggcaatgaa ttcattacct aaggaaggga
     4321 gaatacgaaa cagagcttga ggtaatataa cgtaatacat agattgccac cagctcatcc
     4381 ccaaggaaag agcggcctcc atctggcctt gagcaatgga ttctattcct cctcgcacaa
     4441 tttctcctac ataagctcca ctattgattc ccatagctaa aactccagcc acaaaagggt
     4501 ctaaattcaa acccagagcc ggcaggccaa agtaaaccag gaaaatctgc actaaaagag
     4561 gagtgccccg aatcaactcg atgtaagcac tggaaacatt tcgcagaaaa gagttaggcc
     4621 atactcgcgc caagcctgct cccaaaccaa ttattaaacc cacagctata gaaagagcag
     4681 taacccgtaa agttaccagt gcccctacga gaagtgccgg taatgcttta tatagagcta
     4741 aaaagtcaaa ctgcaaaaat taattcctct cctccaggct actttgcttc tccaaaccac
     4801 ttctggtaaa tctgatcata tttaccagat tctttaatat tttttaaagc ctggttaatg
     4861 gcttctaaaa gatccgtatc ttctttacgc aaagtaactc cgtaattttc ttctccttct
     4921 ttgaaagtag ctacaatctt catatccggt agaaatttca tccggtaagc aacttcagat
     4981 aaatcgttga ccacggcatc agctcttcca ttcatcactt ccatataagc tttgtcggtg
     5041 gtgtcaaatc tctttatctc ttttactccc tctatttcac tcactgccaa atccccagta
     5101 gtcccaattt gcactgctat tactttaccc actaaatctt ccggcccttt aatagagtca
     5161 ttgtctttcc ttactgcaat agcctgggaa gtaacataat aagggtcact catcgcttta
     5221 gatttcagtc tctcttcggt aatggaagtg gcagacatta taacatcaaa ttttttagca
     5281 tccagagcag ggaaaatacc atcccaggag gtgtctataa ataccgcctc tacacctaat
     5341 tcctcagcga tagctctacc taaatcaata tcaaaaccta cgatttctcc gttttcatca
     5401 taaaactcca tagggggata agtagcatca gtgccgatga tgatttttcc tcgagccttg
     5461 atatcatcca gagtagaagc attggctata ccagcaaaaa ccagagagaa gaccagtatt
     5521 cccaaccaaa aaagataatt ccttttcatt tccgctcctc cttttttagg aataatacaa
     5581 cgcccttacc gctgtgtcaa tatagaacta tgagggggca tattcccccc tcataacccc
     5641 cgaaaacaaa gaacataaaa aagaaaaaac tactgggaga gaaagctttc ctcagtggat
     5701 tcctgattaa agcgctcagg aatgacgaga gaagatggat tcccaactga agatttcgca
     5761 actcctttct ttcccttgcc ctcgagggga gagggttagg gtgaaggcga aaactatttc
     5821 aaaccgcctc agtcttaagc gatttagcag gttttgcagg atactataaa tcagggattt
     5881 ttagcttata agaccctctt tttccagtgc ttccctaatg ttagaataac ctaccagtgc
     5941 aacttcccct cgatgttctg atagaaaatc acgagaatga taagaagaga ttactactct
     6001 tttaattccc atcctcgcag actcattaat tcgctgttct aaaagaggaa caggacgaat
     6061 ttccccactc aaccctactt ctcctaaata gattgtttct ttatctattt cccggttcag
     6121 acgagaagaa accagactga aacatactgg aaggtccagg gcagtttctg aggttttaat
     6181 tccccccacc atactgaggt aaacatcctg tcttgagaaa cgtaaacgag tgtttttttc
     6241 aataactgct aaaagcaggt gaagacgatt taaatccata cccaggctta cccgacgggg
     6301 aaaatcaaaa taactaggat gcaccagcga ctggatttcc actaccattg ctctttttcc
     6361 ctctgcccat acagtacgag cagtacctat cccacttaac ttgccttcct tcataaaata
     6421 atgagagggg tctactactt ctactagtcc tttttcctgc atctccagga ttcccacttc
     6481 ctgagtagaa ccaaaacggt ttttcatgct tcgcaaaaga cgcagaggat ggttagcatc
     6541 gccttccaga taaagcacta catctactaa gtgttccaga accttaggtc cagcaatggt
     6601 gccttcttta gtcacatgtc ctactaaaaa gagaacaata ttttcttttt tagctatttc
     6661 cataaagcga gaacttaaat cccggagagt agctacactc ccgggcaaaa attctaaatc
     6721 ttcccgccct aaattctgga tagagtcaac aattaccact tgaggttgaa gagaagaaat
     6781 cccctctctt atttcttcaa aagaattagc agaaagaaga aaaaggggag gattataatc
     6841 caggcctaat cgctctactc gcagataaac ctgataaaga gattcctcac tactaacata
     6901 aagaaccttt tttccctgtc gggcaatacc ttcagcaatt ttcagcagta aagtagattt
     6961 accaatgccc gggtctccac tcagaagaat taaagacccc tctaccacac ctccccctaa
     7021 gacccggtct acctctctta aactggtaga aaaacgctct cccagctgaa tttttttttc
     7081 tttaatttct gacaaagaaa gaggagaaga ataagaagag cttctttctt ttttagtcgc
     7141 ttctcgagag acaggtaaaa aagaattcca ctccccacac tgaggacact tacctaacca
     7201 gcggggagtg gaataaccgc agttactaca acgatagttc atattatcct tttagctttt
     7261 taaagaaagc tccagaggga gtagcttggt gaaattaatc tttccccctt ctactcctac
     7321 cagaattcga tctccttcct taaaaacgcc tttaagcata aggtcagaaa gagggtcttc
     7381 tatcaacttt tgaatggctc gtcgtaaagg tcgagcacca aaattggggt catacccttc
     7441 ctgagcaata agaaaacgag tttcaggagt caggtctatc tccatattgt gttctttcat
     7501 acgtttttga gtttctttca tcataaggtc tactatagct tcgatttctt tctgattcag
     7561 aggtcgaaac acgattattt catctacccg gttcaagaac tcaggagtaa acactttttt
     7621 tacctcttcg gtaacctttt gtttaatctc ctgataagac ataacttctt tttctgacct
     7681 ctcactaaag ccaataccgg actgagaaga aatgagccgg gcccctaagt tagaagtcat
     7741 aatgataatt gtatttttaa aatctaccgc tctaccctga gcgtcggtca ggcgaccttc
     7801 ttccatgacc tgtaataata tattgaaaac atcaggatga gctttttcta tctcatcaaa
     7861 gagaaccacc gaatagggtt tccttctaac tttttcagtt aattgtcctc cttcttcata
     7921 acctacatat cctggaggag agccaattaa acgagaagca gtgaattttt ccatatactc
     7981 tgacatatct aaactaataa gattatcctc tttaccaaag agaaactctg ccagagcttt
     8041 agccagctcg gttttgccca cacccgaagg accgagaaat atgaaagaac taataggacg
     8101 attagggtct ttgagtccgg ctcgggaacg ccgaatagcc cgacataccg cctccactgc
     8161 ttcttcttgc cccactatac gttcatggat ttccttttcc atatttacca atttttcttt
     8221 ttcttcagcg tccaggcgag aaaccgggat accagtccag ctagaaacta ctgtagcaat
     8281 gtcttcttct cccactgaag gtcgggagtt atctacttct ttcagccagc tttctttttt
     8341 atctcgatac ttctttttca acttttcttc ttcatctcga taatgcgctg cttcctcaaa
     8401 atcctgattt ttgaccgcct tttcctttag tttttttatc tcctctgatg ctttttccaa
     8461 ctccttcagg ctttctggta aaaccatagc ttgtagctta acccggctag aggcttcatc
     8521 cataacatca atagccttat caggaagata acggtcagca atatatcgct gggaaagctt
     8581 aactgctgct tccaccgcct gatctgatat ttttactcga tgatgttcct catatctttc
     8641 tttaagacct cgcaatattt ctatgctcac atctaccgag ggctcgtcca cataaacagg
     8701 ttggaatctt ctctctaaag cagaatcttt ttctatatac ttacgatatt ccataatggt
     8761 ggtagcacct atggtctgca attcacctcg agccagagca ggcttcaaga tattggcagc
     8821 atcaatcgct ccttctgctg ctcctgctcc caccaaggta tgaatttcat caatgaaaag
     8881 aactacttcc ttactctgta caatctccga gacaatcttt tttaagcgct tttcaaactc
     8941 tccccggtat ttagtaccag ccactatagc agccaggtcc aaacttatta cccttttacc
     9001 tttcaaaatt tccgggatgt ctccttttac tattttttgt gccagaccct caacaatcgc
     9061 ggtcttaccc actcccggtt ctccaagaag aaccggattg tttttggtac ggcggcttaa
     9121 aatttgtatc actcgctcaa tttctttttc ccgaccaatc accgggtcta acttattttc
     9181 aatagccaat cgggtcaaat cccggctaaa ctcatcaagt accggagtgc gactttctgg
     9241 agaaggatta ttagcttcag gtaagcctcc cgcctcatca ggattttcac ttaaaatttc
     9301 ataaagttta gcccgaactg tttctaagtc taaacctaaa cgctgtaaaa tttgcgctcc
     9361 tactccctct ccttcccgga gaatgcctaa aagaatatgc tctgtaccca cataattatg
     9421 acctaaacga cgagtttcat ccaaagctag ttccaaaact cttttagctc tgggggtaaa
     9481 agctacttca cgagagctct ggtactctct ctgatctaca aaattttcca gctctgctct
     9541 aactaaatcc atggttatat caaaactatc taatatctta gccgcaactc cctctcgctc
     9601 tcgcaacaaa cccagaagaa ggtgttctgt cccaatgtga ttgtgcttga gacgtacggc
     9661 ttcgtcttga gctaagataa tcacttttct cgctctctct gtatatctct caaacatcgt
     9721 tccatatcct ccctaattct attcctaaaa aacccagtac tttcaaactc tgccctctca
     9781 ataacctccc gctcattact ttaccccata gatcaatggt cttcataaac tgaaaacccc
     9841 aactttcagg caatattccc tcactttgag ccaaaagcca aaaagaaaca aaatccaaaa
     9901 attttttttc attttgttct tcattttctt ctatcaattc tttccataaa cattccatca
     9961 tttgccgaaa aatatcctga cgagaagaag actttactaa agatgctcgg gcttgctttt
    10021 cccattccca gacccctctt ccaaaatcct ggatggtctt caaaatttct atttctttaa
    10081 cccccagagt aaaaaggtta gaaaacacaa ataaatgagc taaagcctgg ttactttcac
    10141 catagacctc cactgaaaat ttatcactat ctttaaacca ctgaaaaact ttttcttccc
    10201 catatataaa agaaagagcc ggtaaatgca gcctcatata aatcttgaaa cctgttccca
    10261 gacgcgaagg agaagatgta agatatccca gctcttcact gtaggcatac tccagtttac
    10321 cctcccagaa gtcatcccat ttatttaatt tcttccagta aggctctaaa ctttgatact
    10381 caccttctac aaacaaagat agatggtcct ggtaattaat aatagctccc agatttcctc
    10441 tatgaatcaa gatttttcct gaagaatcag ataataattg agggaaaacc cagctcagta
    10501 actcaagttc tttagaagaa gaacttaact ccacccagga aaaccctctt cgagctaaaa
    10561 aagagtaaac ttctttcgct ctttttttta cttcttcttc ccagagagga gaaaaataaa
    10621 tagaagagga aaagggaaca tgagcaaaat tacgggataa aactagagct actctgttaa
    10681 aattattgag tagcccattt ctccggttca aagagaaccg tttgccaagt gtaaaactca
    10741 ttttagctga gaacgaagct tctccatcac cttttttaaa tgacctgctt tctcatagtt
    10801 ctcttgttct atggcattct tcaaatctcg cccgaggcga aaaatggcat tttgtatttt
    10861 tttctgtctc cgccaattaa taggaatctt gcccctatga cgcaacccca tttgattttc
    10921 agataaccag ctttctatct cttgataata aaaattgtag caatgaggac agcccactac
    10981 tcccctgttt aaaaaatctt cccatctcca ctggcaaaaa gagcaccgtt ccccttcctt
    11041 ttcttctaaa ggaaaaactc tggctaacgg cgaattctct cgcgaaccag aactaaccac
    11101 ccgggaaatg gaaacctgct gggagcagac aggacagaga aaaagcttat ttttaacctc
    11161 tctttctcct cctataaggt aaactactac cttagcctcc ctccacccac agatatcaca
    11221 tttcatttat tccacccatc tcaaaacttg aagaagaatt tctctaaaaa ttcgagaccg
    11281 taatctattc ctgtctgcta ccgaagaaaa atccaaacca gccagttttc tcaaagcatc
    11341 tatagtagct gcccgaatga gagcgccctc acggggagta acaaactcct ctctctctag
    11401 ccagcttact aaatcctcta catcgctctc ttttaaagat tcttcattat catctaagta
    11461 aacaaccacg ggttctaagc ggtcaatagg cactctcctg attctgacat aaccgctacc
    11521 tccacgtcgg ctttctacta taaatcctcg gaatacgtta aaacgggtgg ctaaaacata
    11581 gttgatttga gaaggagcac aatcaaactc atcagctaat ctgccccttt gaatggtaat
    11641 catctgctga ggggaacctt ctattagctt aattatatgt tgttcaatgt catcacatag
    11701 ggagtacatt ctccttccct cctttgacta ttattgactt tctttcatat tatataaaga
    11761 aaactccctg tttgtcaatc caccgaggaa cgaagaagag gaaaaagaat aacttctcga
    11821 atagaaggag aattagtaat caacatcatt aatcggtcaa taccaatacc taaacctcct
    11881 gtagggggca taccatattc cagagcctca atataatctt catcaattaa atgagcttct
    11941 aagtccccct tctctctttt ccgaagctga tctacaaatc tttctttctg ctcaaaaggg
    12001 tcattgagct cactgtaagc attacctact tcttctttac cgatgaaaag ctcaaaacgt
    12061 tctgctaatt gaggattatc ttttttagcc ttagccagag gagaaatttc tactggataa
    12121 tcaaaaacaa aggtgggatt gatcaattga ggctgcacaa atttctctaa aaactcgtta
    12181 acaaatttcc cccggtccca attatcttca tagatcagtc ctcgctcttt agctttagcc
    12241 ctgatagtct ccagagaatc ttccacaatg tctactccca ctaattcctg gatggtttgc
    12301 ggcaagctga ttctgcgcca gggaggagtg aaatctaaag ccatggattg atactctatt
    12361 ttgtaactac cagtaatttt ttttaccact tgtgataaca tttcttccga tagctccatc
    12421 attactttat aatcagcata agcttgataa agttccagca tagtaaactc ggggttgtgt
    12481 cgagtggata taccttcatt ccgaaaacta cgatttattt cataaacttt ctctaatcct
    12541 cctataatta agcgtttgag aaaaagctcc ggggcaatcc gaagataaag ttccattcct
    12601 aaagcattgt gaaatgtttt aaatgggcga gctaaagctc ctcccggaat tacctgcatc
    12661 atgggagttt ctacttccca aaaacctttc tggtctaaaa aaattcgcag ttcttttaac
    12721 agctgagaac ggaggtaaaa aattctcctc acgttgggat tcataatcaa atctaaataa
    12781 cgcttacgat aacgaacttc cacttccttg agtccatgcc atttctcagg aagagggcgc
    12841 agacatttgc taagaagagt aaacttctct accagaacag ttaattctcc ggcatgagtg
    12901 cgaaaaatta tcccttctac ccctattaca tcacctactt ctatttctcg aagaagagta
    12961 aaactatctt ctcctatggt atcttttcga aaataaatct gtattctggc cgtccggtct
    13021 tgcaaatcag caaatatagc tttgccatgc cttctcaatg ccattattct tcctgctatc
    13081 tttactttta catctttatt taccagatta tcagcctgat ttattatttc ttcaatatca
    13141 tgagttttgt agaaactacc attgtagggg tctataccta aatccttcaa aatctggatc
    13201 ttttcccgct tatattcagt attttcttca taccttgttg cttcctcatc catcagttct
    13261 ttactcatcc ttccctttta cctctattat ccggtaatgt gctaccccag caggaacttt
    13321 tatttccact tcttctcctt ttcttttatt aagcagagcc tgtgccaagg gagattcaaa
    13381 ggaaattttt cgagctgagg gatcagcttc taccgaatca acaataaaat attccatttt
    13441 tttattctgg tctaaatttt ctaaaattac ccgtgaaccc agagtaaccc tgtcgctgtt
    13501 acctctctca tcttctacta tctctgcttt tcttagtttt tcttcaatag ataaaatctt
    13561 tccctcaata aaagcctgtt cattttttgc ctcttcatac tcagcatttt cgctgaggtc
    13621 cccaaaagaa agagcatgac ttatcttatc tgctatttct tttcttttca cagtctttaa
    13681 atattttaat tcttcttcta acttttgata cccttcttta gtgaggacaa ttctcccatc
    13741 atcccttttc ttttccatcg ccactacgcc tccagctaaa aaactttatc agatgagaat
    13801 tatagcataa aatataagaa ttaccacaaa ttgtcaaggc ctc
//
LOCUS       DAOSVE010000040        13692 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_2685_length_13692_cov_74.506856, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000040 DAOSVE010000000
VERSION     DAOSVE010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 13692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13692
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2685_length_13692_cov_74.506856"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PK016_04755"
     CDS             <1..428
                     /locus_tag="PK016_04755"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXL02414.1"
                     /translation="EKKRMFRFRSQKKRRTPEINMTPMIDVVLQLVIFFVVTTTFISV
                     ESGAQVNLPSADFSRIEEAKTITVTVTENNSIYIDGALVDEKELAQVAVAELRQNPQT
                     TVVIEADKNVLHGKVVRVMDILKKSGAEKMAIATQPTEG"
     gene            425..1882
                     /locus_tag="PK016_04760"
     CDS             425..1882
                     /locus_tag="PK016_04760"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HXL02415.1"
                     /translation="MMKQPFSEKRIWSFSLLFSIVVNLVLVALISIYWLSFYYFPPPE
                     EERPIVVELMEIPAPKQPVSEAPSQEAEVAELNPLPEVEDKKAELPPQVESEIASEIQ
                     QKKEEVKIAKPPVDLPPAENIIKEASPSGAQDFNPGEEIDVPEDMTWQGVEAESTIKG
                     KIEEAGASTRLSHLAQEGLSKVESTVGETLATGELSAPTAGVEKKSPFTKRPIAVIVE
                     NAPAARPQSGLSKADIVYEILAEGGITRFLAIFNSEPAENVGPVRSARPYFVLKAAEN
                     DAIFAHSGGSVEAFTYLEMLAVDHIDEMKNFQPFWRTQDRPPPHNLYTSVASLRQEAQ
                     RLGYNKPIRVGGFLVGDLGNPGGEKASKLEIDYAADYKVEFTYLPDKNVYQRFINGAP
                     HIDAAAGRAIQCSTIIVQVAEHQVKDEEGRLEIRFVGEGKGWVFRDGKVAPLRWEKND
                     LRDKTRFYLADGEELKINPGKVWIEVVDTRTKVVF"
     gene            1882..2634
                     /gene="lgt"
                     /locus_tag="PK016_04765"
     CDS             1882..2634
                     /gene="lgt"
                     /locus_tag="PK016_04765"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HXL02416.1"
                     /translation="MYPVMFHLGSFPIYSYGVMLAIAILVGIFYAMRRAPRYQVSSDA
                     VVEVSALCIIGGVVGARLLYVLINWEYYRGNLAHIFLVREGGLTFYGGIAGALLLIIP
                     YVHYKKYSLPAFFDLFSPPLALGYAIARVGCFLNGCCYGRVCPFSSFPLAVQFPHLSG
                     FRYPTQLYSVGYSLVILFILLRLEKNKAFAGELFLDYLWLYGIARFLIEYLRDEPFSV
                     GGIFTLGQTACLLIIISSLTLREVIKRYGKKP"
     gene            2618..3520
                     /gene="xerD"
                     /locus_tag="PK016_04770"
     CDS             2618..3520
                     /gene="xerD"
                     /locus_tag="PK016_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HXL02417.1"
                     /translation="MGRNLDEWVEAFLDFLFWEKRAADNTILSYGQDISSFVQFLKEK
                     KIISFGEVTFLVLEEFVSRESEKELETTSLARKISALRTFFSFLVREKEVPENLARLL
                     DAPRLERNLPQVLSLEEVETLLSACDKIKPRGLRDRAILELLYSSGLRVGEVVSLEFS
                     HLDLENRMLRLWGKGFKERLVPFGEEAYQAISLYLEKGRDEILKGRSSNYVFISGPSA
                     GPLTRQSVWNMVKRYAKQANLDKDVTPHTLRHTFATHLLENGADLRVVQEFLGHSDIS
                     TTQIYTHVSKRILRETYDRAFPRK"
     gene            3538..4365
                     /locus_tag="PK016_04775"
     CDS             3538..4365
                     /locus_tag="PK016_04775"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HXL02418.1"
                     /translation="MDDMKEKLEESYQFIKEKIDFVPRVGIILGTGLGALVDDIEIDR
                     VIPYEEIPHFPISTVETHRGNLVLGHLEGKAVVVMQGRFHYYEGYDMKQITFPVRVMR
                     KLGAEVLLVSNAAGGMNRYMKRGDLMIIRDHINLLGDNPLRGPNDPELGPRFPDMSQA
                     YDRELAELALQVALENGIYVHQGVYVALAGPNLETPAEYRFLITIGADAVGMSTVPEV
                     IVARHSGMKVLGISCITDLAIDGVVEEVSFEDIVGAANSAEPKLTLITREVVKRLTL"
     gene            4367..4828
                     /locus_tag="PK016_04780"
     CDS             4367..4828
                     /locus_tag="PK016_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HXL02419.1"
                     /translation="MIRKLLEVALLAALPVSEIRGALPYGVLVRNLPFSWVVVVSFIF
                     NLLPFFLVMFALSFFLKFLLRWEWFGNFWYKYITRAQNQFRNYEKWGKWGIMLFVGIP
                     LPFTGVWTGSIVCFIAGLSWKESLPFVLGGLAMATFLVSLAVLGGNYLLTG"
     gene            4837..5889
                     /gene="mtnA"
                     /locus_tag="PK016_04785"
     CDS             4837..5889
                     /gene="mtnA"
                     /locus_tag="PK016_04785"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011994667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HXL02420.1"
                     /translation="MLKAVEWKGESFRFLDQTLLPLEEIYIETADYREVGEAIRRLKI
                     RGAPLLGVAVAYGLCLGALEGERNKDKDFFDFLEEVDRVLRLTRPTAVNISWSLNRLK
                     KIWERGKKEDWLPHEVVKALIDEAKKIEEEDYKNNEKIAQNGAQLFQDGDKVLTHCNT
                     GSLACSGWGTALGAISWAVLQEGKKIQVYADETRPLWQGARLTAFELVKNSIPVTVIC
                     DNMAGYLMSQGLIDKVIVGADRVTLQGDAANKIGTYSLAVLSHYHKIPFYMAFPLSSV
                     DFSIREGKEIPIEERNPEEVVAPLGMRIAPPGVGVRNPAFDVTPHHLITALVTEKGVI
                     YPPFEERLRNWKAGVK"
     gene            5891..7045
                     /locus_tag="PK016_04790"
     CDS             5891..7045
                     /locus_tag="PK016_04790"
                     /inference="COORDINATES: protein motif:HMM:NF037429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02421.1"
                     /translation="MDNMITIEDVRKLLEFKSGETLVTSFYLNIDPRRVSPSKITTLT
                     RNLLREKQQALEKENWPEELLNKVKEDFKRIEDFVVNHFELKGKVKGLVIIADAARNF
                     FQVYRVAQPVKSRVVVDSDPYIRPLVAILDEYHRLMLVLLDQREARLLEVYMGEILEY
                     EAYYSDVRGKVKEGGWYGLEERRIERSIKNKVIHHYQRVADAILEHFRMGHFEYLFVG
                     MKVEDYSLFFPLLHSYVQPRVKGRLDVGPKDNISTIIEVALQAERKIEEEEDNKILEH
                     LVETLNRGGLAAKGIDNVLRSIPLGACQVLVVDDNYHIPGYVCEDCAYLALEGGICPF
                     CRKEALPVEDVIEEAMEEVLLQRGEIKYITTDNPRLEEISRIGALLRYKI"
     gene            7403..8368
                     /locus_tag="PK016_04795"
     CDS             7403..8368
                     /locus_tag="PK016_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HXL02422.1"
                     /translation="MSRNGWLILVLIAFILILFYFLARKKNSSFSNLFKNLARLFNHL
                     SAIDILVALGGLVVGLVIGVLLTYPISFLPFKNASLPLIVTLVCGILGLAVFMWRKED
                     IVTIFTGIPRLKSPRGGGNSGKILDTSAIVDGRIADICKTGFVEGDLLIPRFVLRELQ
                     QVADSQDPLKRNRGRRGLDILNRMRKEKKVNVRIIDRDFPEIRDVDAKLIKLAKVLGT
                     KVITNDFNLNKIAELEGVEVLNINDLSNALKPYVLPGEELRVQVIREGKEENQGVGYL
                     DDGTMVVIEEGRKYINSTIDTVVTSVLQTPAGRMIFAKLKGSSHP"
     gene            8394..9086
                     /gene="ispD"
                     /locus_tag="PK016_04800"
     CDS             8394..9086
                     /gene="ispD"
                     /locus_tag="PK016_04800"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HXL02423.1"
                     /translation="MFFAIIVAAGRGERVNSLIPKQYLPLLGKPILAHTLDIFEKSPW
                     IGEIIVVINPLHEEIFKKEVWEKYGYRKIKKYVSGGETRQDSVYCGVRELKDKSADFV
                     LIHDGVRPLVDEKILERCVEGVTKFEAVCCALPCADTIKRSLDGEIIDETLDRQKIWR
                     AQTPQAFRISLIWEALEKAKEEGFQGTDDSSLVERMGKSVHLVLGSEDNLKITTPQDL
                     ILAEGLLRWRKK"
     gene            9083..9568
                     /gene="ispF"
                     /locus_tag="PK016_04805"
     CDS             9083..9568
                     /gene="ispF"
                     /locus_tag="PK016_04805"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HXL02424.1"
                     /translation="MSSWRVGLGYDIHPLQAGKKLFLAGVCIPCERGLHGHSDGDVLC
                     HALMDSLLGAAGLQDIGHWFPPTSSSTEGVKSILLLEEVCRKIREKGWEIENVDTTLI
                     AEEPLIAPFIGKMKETLSLTMGLNEEAIGIKATTNEGLGSLGKGEGIGCFAVSLLRRK
                     E"
     gene            9532..10281
                     /locus_tag="PK016_04810"
     CDS             9532..10281
                     /locus_tag="PK016_04810"
                     /inference="COORDINATES: protein motif:HMM:NF015298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HXL02425.1"
                     /translation="MFCCKPFAKERINKILALVVCGLVITAPGASSGGEELISLESQS
                     GACSINVGGRTILRVRYGGGYSTLEERAKVIAQSLAKLFQNSQKVVNFYVVWEKGRAI
                     AFADQNKLFTVLKEDARVNKSDPLSLALSWLNNVQMEFYRVREKKEVEEVMEGIASWC
                     SNGFEGRTTASGEIYTPYAFTAAHRTFPMGSVLLVTNLEDGRKVVVRVNDRGPWREGR
                     IVDLSLAAAKVLDIYEDGLSEVKVEVLRWGE"
     gene            10269..11714
                     /gene="gltX"
                     /locus_tag="PK016_04815"
     CDS             10269..11714
                     /gene="gltX"
                     /locus_tag="PK016_04815"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HXL02426.1"
                     /translation="MGRVRTRFAPSPTGFLHLGGARTAAFNWALARKEKGDFILRIED
                     TDRERSKKQFEEAILEDLRWLGIEWDEGPDRGGPYAPYRQSERGEIYQFFIAQLKAKN
                     FIYPCYCTVEELEEERKKALKVGKAPRYSGRCFFLSPEERKEKEEKGIKPSWRFRVPG
                     GKEIMLEDLVRGKVAFHTNSLGDFIVLKSDGVPTYNFACVIDDALMNISHVLRGEEHL
                     PNTPYQILLYQALELTPPLFAHVPIILDEKRTKLSKRRGEVNLQFFREEGFLPEAILN
                     YLLTLGHSFPEGREIIEEEELYTLFSLERIGKGGAIFDLSRLNWWNKTYIRQMDIKKL
                     EEKLLPWGGDEIKKFKRELGEERFYLLLDLIREESVTLKEIAEKLRDYVSVKREKVED
                     KEKLKILKFLLSRLGELATAWREEEIQKNLRLWQKESGFPPSLFYPVLRWALTGAEEG
                     PELDRLLVALGKDKVLEKIKARVGDNGNSTV"
     gene            11695..13116
                     /gene="cysS"
                     /locus_tag="PK016_04820"
     CDS             11695..13116
                     /gene="cysS"
                     /locus_tag="PK016_04820"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HXL02427.1"
                     /translation="METQLFNTLTQKKEAFFPQEGKLVRFYVCGPTVYDLIHIGNARV
                     FVVFDSLRRFLEHKGFEVVMVQNFTDIDDKIIKRAEELGESPRELAERYIAEYWHDAE
                     TLKIRKATFHPRATEHIGDIIGLIKTLEEKGYTYEREGDILFDVSRFPSYGKLSRKNL
                     DELVEGARVEVRYKKKNPADFVLWKKAKPGEPSWESPWGRGRPGWHIECSAMSMKYLG
                     PTLDIHAGGIDLIFPHHENEIAQSEAATGKPFARFFLHNGYVNINQEKMSKSLGNIVT
                     VRELTQKYEPSVLRLAIFSAHYRNPINLDEELLQSSRQFGERIKNFLRNINFIGGKIK
                     NNPAFLKEDEEEYQKIDEFYRDFQKALADDFNTPQALGVLGEFLRLFNQYLSPRRGKW
                     DVQVLEKIRDFLEVVEEILGILPGKEMERGDEIIKELVEEREKARKDKDWKKADVLRE
                     EIKSRGYIVEDTPLGPRFFKVEE"
     gene            13113..>13692
                     /locus_tag="PK016_04825"
     CDS             13113..>13692
                     /locus_tag="PK016_04825"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HXL02428.1"
                     /translation="MMKSELIYGRQPVREALKSEKRKVEKLFIMEGAQGKIIEEIKQL
                     SWKRKIPWEIKDKTFLEELVGKNVNHQGVVLLAQPRVSPSWQEILEKNKASREATVIF
                     VDRVEDPANLGAIIRTAAFFGVEGILISKARSAPLDSPVVRASAGGIESIDIAQINNF
                     TQVIKEFQEAGFWIVALEADGDKDIWDADLRGM"
BASE COUNT         4053 a         2207 c         3388 g         4044 t
ORIGIN      
        1 aggagaagaa gagaatgttc cggtttcggt ctcagaaaaa gagacgaact cctgagataa
       61 atatgactcc tatgatagat gtggttttgc aactggtgat tttttttgta gtcactacca
      121 cttttatcag tgtagaaagt ggggcacagg tgaatctgcc ctcggctgat ttttctcgaa
      181 tagaagaagc aaaaactata acggtaactg taactgaaaa caattctatt tatatagatg
      241 gtgctctggt ggatgaaaag gaactggctc aggttgcagt agcagaactc cgccaaaatc
      301 ctcaaactac agtggttatt gaagcagata aaaacgttct tcatgggaaa gtggtaaggg
      361 tgatggatat tttgaaaaag tcgggagcag agaaaatggc cattgccact caacccacgg
      421 agggatgatg aaacaacctt tcagtgagaa gagaatatgg tctttttctc ttcttttttc
      481 tatagtggtt aatttggttt tagtagcgct aatatccatc tattggcttt ctttttacta
      541 ttttcctcct ccggaggagg aacggccgat agtggtggaa ctgatggaga ttccagctcc
      601 caagcagcca gtaagcgagg ctccgtctca agaagcagaa gtggcagaat taaaccctct
      661 tccggaagta gaagataaaa aagcagagct acctccacag gtagaaagcg aaattgcttc
      721 agagatacag caaaaaaagg aagaggtaaa aatcgccaaa cctcctgtag atttgcctcc
      781 agcggaaaac ataataaagg aagcttctcc ctctggtgct caggacttta atcctggaga
      841 agagattgat gttccggaag atatgacctg gcaaggagta gaggcagaat caaccattaa
      901 aggaaagata gaagaagcag gagcttccac tcgtctatcc catctggctc aggaaggatt
      961 gagtaaagta gaaagcacag tgggagaaac tttagctacg ggggagctct ctgctcctac
     1021 tgcgggagtg gagaaaaaat ctccttttac taaaagacct attgctgtga ttgtggaaaa
     1081 tgctccagcg gctcgtcctc aatcgggttt gagtaaagca gatatagttt atgaaatctt
     1141 agctgaggga gggattactc gttttttagc tatttttaac tctgaaccag cagaaaatgt
     1201 agggccggta cgtagtgccc gtccttactt tgtgcttaaa gcagcagaaa acgatgccat
     1261 ttttgctcat agtggaggaa gcgtagaagc atttacttac ttagaaatgt tagcagtaga
     1321 ccatatcgat gagatgaaaa attttcagcc tttctggaga actcaagacc gtcctcctcc
     1381 tcataatctt tatacctctg tggcttctct gcgtcaggaa gcgcagagat tgggttataa
     1441 taagccgatt agagtaggtg gctttctggt tggcgattta gggaatccag gaggagaaaa
     1501 agcttcaaaa ctggagattg actatgctgc cgattataaa gttgaattta cttatcttcc
     1561 tgataaaaac gtttatcagc gttttataaa tggtgcacct cacatagatg cagcagcggg
     1621 ccgggctatt caatgttcta ccattattgt tcaggtagcc gagcaccaag taaaagatga
     1681 agagggaagg ctggaaataa gatttgtggg ggagggaaag ggatgggttt ttcgagatgg
     1741 taaagtagca cctctgcgct gggagaaaaa tgacctccgg gataaaacca gattttacct
     1801 tgccgatgga gaagagttaa aaattaatcc ggggaaagtg tggatagagg tagtcgatac
     1861 tcgtactaag gtggtgtttt gatgtatcct gttatgtttc atttaggaag ttttcctatt
     1921 tattcttacg gggtgatgtt agcaatagca attctggtgg gtatttttta tgctatgcga
     1981 cgagcgcctc gctaccaggt atcttctgat gcagtggtgg aagtgagtgc cttatgtatt
     2041 ataggaggtg ttgtgggagc gaggttactt tatgttttaa taaactggga atattaccgg
     2101 gggaatttag ctcacatttt tttggtgcga gaaggagggc taacttttta tggagggata
     2161 gccggtgctc ttcttttaat tattccctac gttcattata aaaagtattc ccttcctgct
     2221 ttttttgatc ttttttctcc tcctcttgcc ctgggttacg ctattgctcg ggtgggatgt
     2281 tttctaaatg gctgttgtta tggccgagta tgtccttttt cttcctttcc tttagcggta
     2341 caatttcccc atctttctgg gtttaggtat cctacccaac tctattcggt aggctattct
     2401 ctggtaattc tttttatcct cttacgttta gaaaaaaata aagcttttgc cggtgaactt
     2461 ttcctcgatt atctatggct ttatggtatt gctcgttttt tgattgaata tttaagagat
     2521 gaacctttct cggtgggagg aatttttact ctgggacaga cggcgtgctt gctaatcata
     2581 atttcttcgc taactttgcg agaggtcatt aaaaggtatg ggaagaaacc ttgatgagtg
     2641 ggtagaagct tttttagatt tccttttctg ggagaagaga gcggcagata acactatact
     2701 tagttatggt caggacattt catcgtttgt ccagtttttg aaagagaaaa aaattatctc
     2761 ttttggggag gtaactttct tggtgttgga ggagtttgtg agtcgggagt cagagaaaga
     2821 gctggagact acttctttag ctcgaaaaat ttctgcactg cgcacttttt tttcttttct
     2881 ggttcgagaa aaagaagtac ccgagaattt agcccggctt ctggatgccc ctcgattaga
     2941 aagaaatctt ccccaggttt tgagtctgga agaggtggaa actttgcttt cggcttgtga
     3001 taaaatcaaa cctcggggat tgagagaccg ggcaattctt gaacttcttt attccagtgg
     3061 tttacgagtg ggagaggtgg tctctttaga attttctcat ctggacctgg aaaatagaat
     3121 gttgaggttg tggggtaaag gttttaaaga aagactggtc ccttttggag aagaagctta
     3181 ccaggctatc tctctttacc ttgaaaaggg gagagatgag attttgaagg gtcgctctag
     3241 taattatgta tttataagtg ggccttccgc tggtcccttg actcgtcaga gtgtctggaa
     3301 tatggtaaaa agatatgcca aacaggctaa cctagataag gatgttactc ctcatacctt
     3361 gaggcatact tttgctactc atcttctgga aaatggcgct gatttaagag tggtacagga
     3421 gtttctagga cacagcgata tttctactac ccagatttat actcatgtga gtaagagaat
     3481 tttgcgggaa acctatgatc gggctttccc tcgaaaatag aaaaggaggt agataacatg
     3541 gacgatatga aagaaaaact tgaagaaagt taccagttta ttaaggaaaa aattgatttt
     3601 gttcctcgag tgggaattat actgggaaca ggtttagggg ctttggtaga cgatatagaa
     3661 attgaccggg tgatacctta tgaggaaata ccccattttc caatttctac tgtagagact
     3721 catcggggaa atctggttct ggggcattta gagggtaaag cagtggtggt aatgcaaggg
     3781 agatttcact attatgaagg atacgatatg aagcaaataa cttttcccgt ccgggtgatg
     3841 agaaagttgg gagccgaagt tcttctggta agtaacgcag caggaggaat gaaccggtat
     3901 atgaaaaggg gagatttgat gattataaga gaccacatca atctcttagg agacaatcct
     3961 cttcgtggtc ccaatgaccc ggaattagga ccacgttttc ctgatatgtc tcaggcctat
     4021 gatagagagc tcgcagagct tgctttacag gttgctctgg agaatgggat ttatgtacat
     4081 cagggagtat atgtagcttt agctggtcca aacttagaga ctccggctga atatcgcttt
     4141 ttaatcacta ttggtgctga cgcggtaggc atgtctaccg ttcctgaggt aattgtagct
     4201 cgtcactcag ggatgaaagt tctgggaatt tcttgtatta ccgatttagc tattgatggc
     4261 gtggtagaag aagtgagctt tgaagatata gtaggggcgg ccaacagtgc tgaacccaag
     4321 cttactttaa ttacgagaga agtagtaaag agattaaccc tttaatatga tacgaaaact
     4381 actggaggta gcattgcttg ctgctttacc ggtgagtgag attcggggag ctctccctta
     4441 tggagtgctg gtgagaaatt tacctttttc ctgggtagtg gtggtgtctt ttatttttaa
     4501 ccttctacct ttttttctgg tgatgtttgc tctgagtttc tttttgaaat ttctccttcg
     4561 ctgggaatgg ttcggtaatt tctggtataa atatataact agggcacaaa atcagttcag
     4621 gaactatgaa aaatggggta aatggggaat aatgttattc gtaggcatcc ctcttccttt
     4681 tactggggta tggaccggtt caatagtttg ttttattgcc ggattatcct ggaaggagtc
     4741 tttgcctttt gttctgggag gtttagctat ggctactttc ttggttagct tagcagtgtt
     4801 gggtggtaat tatttattaa cagggtaggt gatattatgc taaaagctgt agaatggaaa
     4861 ggcgagagtt ttcgattttt agatcagact cttttacctc ttgaagagat atacatagag
     4921 acagctgatt accgagaggt aggagaagct atccgtcggt tgaaaattcg gggagctcct
     4981 cttttaggag tggctgtagc ttatggtttg tgcctcggag ctttagaagg agagagaaat
     5041 aaagataaag acttctttga ttttttagaa gaggtggata gggttttacg tcttacccgt
     5101 ccgacagcgg tgaatatctc ctggtcttta aaccgcttga agaaaatctg ggaaagagga
     5161 aaaaaagagg attggctccc tcatgaagtg gttaaagcac tcattgatga ggcgaaaaaa
     5221 atagaagaag aagattacaa aaataacgaa aaaattgccc agaatggcgc tcagctcttt
     5281 caagatggag ataaggtgct tacccattgc aataccggct ctctggcctg tagtggatgg
     5341 gggacagctt taggggcaat tagctgggca gttttgcaag aggggaagaa aatccaggtt
     5401 tatgctgatg aaactcgtcc tctatggcag ggagctcgac ttactgcttt tgagctggta
     5461 aaaaattcta ttccagttac tgtaatatgt gataatatgg ctggttattt aatgtctcaa
     5521 ggattgatcg ataaagtgat agtaggagca gaccgggtta ctttacaggg tgatgcagct
     5581 aataaaattg gtacttattc tttagctgtt ctatctcact atcataaaat acctttttat
     5641 atggcttttc ctttatccag tgtagatttt tctataagag agggcaaaga aatccccatt
     5701 gaggaaagaa acccggaaga agtagtagct cctttgggga tgagaattgc tcctccgggg
     5761 gtaggagtta gaaaccctgc ttttgatgtt actccacatc atttaatcac tgcgcttgtt
     5821 actgagaaag gtgttattta tcctcctttt gaggagagat tacggaattg gaaagcgggg
     5881 gtaaaataaa atggataata tgataactat agaagatgtt agaaaacttt tagaatttaa
     5941 gtctggggag actttggtca cttcctttta tcttaatata gatcctcgtc gggtttcccc
     6001 tagcaaaatt accactttaa ctcgtaactt gctccgggag aaacaacagg ctctggaaaa
     6061 agaaaactgg ccagaggaac tgttgaataa ggtaaaagaa gattttaagc ggattgagga
     6121 ttttgtagtt aatcattttg agctaaaggg gaaagtgaaa ggtctggtaa taatagctga
     6181 tgcagcacgc aatttttttc aggtctatag agttgctcaa ccggttaagt ctcgggtagt
     6241 ggtagactct gacccttata ttcgtcctct ggtggcgatt ctggatgaat atcatcggct
     6301 gatgctggtt cttctcgatc agcgagaagc tcgtttactt gaagtgtata tgggggaaat
     6361 attagagtat gaagcttatt attccgatgt tcgaggaaaa gtaaaagagg gaggatggta
     6421 tggcttagag gagcgccgaa tcgagaggag tattaaaaat aaagtcatac atcattatca
     6481 aagagtggcc gatgctattc tggaacactt tagaatggga cattttgagt atctttttgt
     6541 aggtatgaag gtagaagatt attctctttt ctttcctctt ctccatagct atgtgcaacc
     6601 cagggtgaaa ggaaggttag atgtcggtcc taaagataac atcagtacta taattgaagt
     6661 ggcgctacaa gcggagagaa aaatagaaga agaggaagat aataaaattc tggaacatct
     6721 cgtggaaacc ttaaatagag gaggactagc tgctaaaggg atagataatg ttttgcgttc
     6781 tatacctctc ggagcctgcc aggttctggt ggtagatgat aactatcata taccaggata
     6841 tgtttgtgaa gattgcgctt acttggcttt agaaggagga atttgtcctt tttgtaggaa
     6901 ggaggctctt ccagtggaag atgtgataga agaagctatg gaagaagttc ttttacaaag
     6961 aggagaaatt aaatatataa ctaccgataa ccctcggtta gaagaaatct cccgcattgg
     7021 agctcttttg cgatacaaaa tttaaaattt tttatgcttt ttgtggaaag ttgcaggagc
     7081 gacgaatctc tctgtggctt tccacccctt cattgtgagt gctgagagag tagctttaag
     7141 ttttttgggg tcttttattg gagccacaaa aggtctttcc tcagtagtct ttactctttt
     7201 agttttttgc cttccttttg tttttagtgc cgtaggcagt tctgtgttta tacaaaggag
     7261 agttataatt atgtcattat cagtaaataa tcgatatatt ctgataaaaa aggcgggaaa
     7321 ctatattaat cggaattttt tttctgtaaa atgatataat cttttagctg taaggcaatt
     7381 accttagcaa ggaggaaatt ttatgtctcg aaatggatgg ttaatattag ttttaatagc
     7441 ttttattcta attttatttt attttcttgc ccggaaaaaa aatagttctt tttccaattt
     7501 attcaaaaac ttagctcgac tctttaatca tctttcagct attgatattt tagtggcttt
     7561 aggaggattg gtagtaggtt tagtaattgg agtgcttctt acttacccta tttctttttt
     7621 accttttaaa aatgcttctc ttcctctcat tgttactctg gtttgcggga ttttaggttt
     7681 agcagtattc atgtggagaa aagaagatat tgttaccatt tttactggta ttccccgatt
     7741 gaaatctcct cgaggaggag gtaattcagg taagatttta gatactagcg ccattgtaga
     7801 tgggaggatt gctgatatat gcaaaactgg ttttgtggaa ggagaccttt taattccccg
     7861 ctttgtttta cgggaacttc agcaagtggc tgattctcaa gaccccttaa agaggaatcg
     7921 aggaaggaga ggtttagata ttcttaaccg aatgaggaaa gagaagaaag taaatgttag
     7981 aattatagac cgagattttc cggaaataag agatgtagat gccaaactga taaaattagc
     8041 taaagtactg ggaacaaaag tcattaccaa cgactttaat ttaaataaaa ttgctgaatt
     8101 agaaggggta gaggtattga atattaatga cctttctaat gctctaaaac cctatgtcct
     8161 tcccggggaa gagttgcgag tgcaagtcat ccgggaaggt aaagaagaaa atcaaggagt
     8221 gggctatctc gatgatggca ctatggtagt gatagaagag ggaagaaagt atattaattc
     8281 tactatagat acagtagtta ctagcgttct acagactcca gcaggtagaa tgatatttgc
     8341 gaaattaaaa ggtagcagtc acccttgagg taatctggaa aggggaatga attttgttct
     8401 tcgctataat agtagcagct ggtagaggag aaagagtaaa ctcgttaatt cccaagcaat
     8461 atttaccact tttgggaaaa ccaattcttg ctcatacttt agacatattc gaaaagtccc
     8521 cttggatagg ggaaataata gtagtaatta accctctcca tgaggagata tttaaaaaag
     8581 aagtgtggga aaaatatggt tatcgaaaaa ttaaaaaata tgtttcaggt ggagagactc
     8641 gccaggactc ggtttattgt ggagtccggg agctgaaaga taaaagtgcg gattttgtac
     8701 tcatccatga tggggtgaga cctctggtag atgagaaaat attagaaagg tgtgtggaag
     8761 gagttaccaa gtttgaggct gtttgttgtg ctctaccttg tgctgatacc attaagcgca
     8821 gtcttgatgg agaaattatc gatgagactc tggatcggca aaaaatctgg cgagcgcaaa
     8881 ctcctcaagc ttttcgtatt tccttaatct gggaagctct agagaaagct aaagaggaag
     8941 gatttcaagg aaccgatgac tcatcactag tggagagaat ggggaaatcg gtacatctgg
     9001 tgctgggttc agaggataac ctgaaaatta ctactcccca ggaccttatc ttagctgaag
     9061 gattgttgag gtggcgaaaa aagtgagtag ttggcgagtg ggtttggggt atgacattca
     9121 tcctttacaa gcaggaaaga agttgtttct ggccggggta tgtatacctt gtgagcgagg
     9181 gctacatggt cattctgatg gagatgtact ctgccatgcc ttgatggata gccttctggg
     9241 agctgctggt cttcaggata ttggacattg gtttcctcca acttcatctt ccacagaagg
     9301 agtgaaaagt attctgcttc tggaagaggt ttgtagaaaa atacgagaga aaggctggga
     9361 gatagaaaat gtggatacta cccttattgc cgaggaacct cttattgctc cctttattgg
     9421 aaagatgaaa gaaactcttt ccttaaccat gggattaaac gaagaagcta taggcataaa
     9481 agctactact aatgaagggt taggtagtct gggaaagggg gaaggaatcg gttgttttgc
     9541 tgtaagcctt ttgcgaagga aagaataaat aaaattttgg ctctggtagt ctgtggatta
     9601 gtaattactg ctcctggagc aagcagcgga ggagaagagt tgatttctct tgagtctcaa
     9661 tctggcgctt gctctataaa tgtgggaggt cgaaccattc ttcgcgtccg ctatggagga
     9721 ggatattcta ctttagaaga aagagctaag gtgattgctc aatccctggc taaattattt
     9781 caaaattccc agaaggtggt aaatttttac gtggtctggg aaaagggtag agctatagct
     9841 tttgctgacc agaataaact ttttaccgtc ttgaaagaag atgctcgggt taataaatct
     9901 gaccctctaa gccttgctct ttcctggcta aataatgtcc agatggaatt ttatcgagta
     9961 agagaaaaaa aagaggttga agaagtgatg gaagggatag cttcctggtg cagtaatgga
    10021 tttgaaggaa gaacgaccgc cagtggagaa atttacactc cttatgcttt cactgctgct
    10081 catcgtactt tccctatggg gagtgttctt ctcgtgacta atctagaaga tgggcggaaa
    10141 gtggtggtaa gggtgaatga ccgtggtccc tggagggaag gtcgaattgt ggacctgtct
    10201 cttgctgctg ctaaagttct ggacatttat gaagatggtc tgagcgaggt taaagttgag
    10261 gtgttaagat ggggagagtg agaactcgct ttgctcccag tcctaccgga tttctacatc
    10321 tgggaggagc aagaacagct gcttttaact gggctttagc tcgcaaagaa aaaggagatt
    10381 ttattttacg cattgaagat accgaccggg aaaggtcaaa aaaacaattt gaagaagcta
    10441 tattagaaga cctccgatgg ctgggtatag aatgggatga aggtccagac cgaggaggac
    10501 cttatgcccc ttataggcag tcagaaagag gagaaattta tcaatttttt attgctcagt
    10561 taaaagctaa gaactttatt tatccttgct attgcacggt ggaagagttg gaagaagaga
    10621 gaaaaaaagc gttaaaagtg ggtaaagctc cccgttattc cggtcgttgt ttttttcttt
    10681 ctcccgagga gagaaaagaa aaagaagaaa agggaataaa gccctcctgg cgcttccgag
    10741 ttccgggggg taaggaaata atgctggaag atttggtaag agggaaagtg gctttccaca
    10801 ctaattcttt aggagacttt atcgttttga agtcagatgg tgttcctact tataattttg
    10861 cctgtgttat agatgatgct ttgatgaata tctctcatgt attgcgagga gaagagcact
    10921 tacctaatac tccttatcaa attttacttt atcaagcttt agagcttact cctcctcttt
    10981 ttgcccatgt acctatcatt ctagatgaaa agagaactaa gttgagtaaa agaagaggag
    11041 aagtaaattt gcaatttttt agagaagaag gatttttacc tgaagctatc ttgaattatc
    11101 ttctgacttt aggacattct ttccctgagg gaagggaaat aatagaggaa gaagaactct
    11161 ataccctctt ttctttagag cgaataggta aaggaggggc gatttttgac ctttcccgtt
    11221 tgaactggtg gaataaaacc tatattcgcc agatggatat taaaaaatta gaggaaaaac
    11281 tcctcccctg gggaggggat gagataaaga aatttaaaag agaattagga gaagaaaggt
    11341 tctatctatt gctggattta ataagagaag agtcagttac cctgaaggaa atagcagaaa
    11401 agttaagaga ctatgtatca gtaaaaaggg aaaaagtaga agataaagaa aagctcaaaa
    11461 tattaaagtt tctgctttct cgtttgggag agcttgctac tgcctggaga gaagaagaga
    11521 tacagaaaaa ccttcgtctc tggcagaaag agagcgggtt ccctccctct cttttttatc
    11581 cggttttgcg ctgggcgtta accggggctg aagagggacc ggaattagac cgcttacttg
    11641 tggctttagg aaaagataag gttttagaaa aaattaaagc aagagtggga gataatggaa
    11701 actcaactgt ttaatactct tactcagaaa aaagaagcct ttttccctca ggaagggaag
    11761 ctcgttcgct tttatgtttg tggccctact gtttatgatt taatccatat tggaaatgcg
    11821 cgcgtttttg tggtttttga ctctctgcgt cgttttttag aacataaggg cttcgaagtt
    11881 gtaatggtac agaatttcac cgatattgac gataaaatca taaaaagagc agaagagcta
    11941 ggggagagcc ccagggaatt ggccgagcgt tacattgctg aatactggca cgatgctgaa
    12001 accttgaaaa tacgaaaagc cacctttcat cctcgagcta cagaacatat tggagacata
    12061 attggtctca ttaagacttt agaagagaaa ggttatactt atgagagaga aggagatatc
    12121 ctttttgatg tttctcggtt tccttcttat gggaaacttt ctcgcaagaa tcttgatgag
    12181 ttagttgaag gagctcgggt agaggttaga tataaaaaga aaaatcctgc tgattttgta
    12241 ctgtggaaaa aagctaaacc tggtgagcct tcctgggaaa gtccctgggg gagaggtagg
    12301 ccgggatggc atattgagtg ctcggccatg tctatgaaat atttagggcc tactttagac
    12361 attcatgctg gaggaataga cttaatattt cctcatcatg aaaatgaaat tgctcagagt
    12421 gaagcagcta ctggtaaacc atttgctcgg ttttttcttc acaatggtta tgtcaacatc
    12481 aaccaggaaa agatgtccaa atctctgggt aatattgtta cggtacgaga gcttactcaa
    12541 aaatatgaac cttcggtttt gaggttagct attttttctg ctcactatcg taaccccatc
    12601 aacttagatg aagaactttt gcaatcttct cgccagtttg gagagaggat taaaaatttt
    12661 ctccgtaata taaattttat aggaggaaaa ataaaaaaca atcctgcttt tttaaaggag
    12721 gacgaagaag agtatcagaa aatagatgaa ttttaccggg actttcaaaa agctctggct
    12781 gacgatttta atacccctca agctctgggg gtgctgggag aattcctccg tcttttcaat
    12841 caatatctct ctccccgtcg gggaaagtgg gatgtgcaag tattagagaa aattagagat
    12901 tttctggaag tggtagaaga aatattagga attctcccgg ggaaggaaat ggaaagaggg
    12961 gatgaaataa taaaggaact ggtggaggaa agagagaagg caaggaaaga taaggattgg
    13021 aaaaaagcag atgtactgcg agaggaaata aaaagtagag gatatatcgt ggaagatacc
    13081 cccctgggtc ctcgcttttt taaagtggaa gaatgatgaa gtcggagctt atttatggac
    13141 gccagccggt gagagaggct ctgaagtcag agaaaagaaa agtggagaaa ttgtttataa
    13201 tggaaggggc acaggggaaa ataatagaag aaatcaaaca gctgtcttgg aaaaggaaaa
    13261 ttccctggga aataaaagat aaaacttttt tagaagaatt agtggggaaa aacgttaacc
    13321 accagggagt agtattatta gctcaacctc gggtatcacc ctcctggcag gaaattctgg
    13381 agaaaaataa agcctctcga gaagcaacag taatatttgt agaccgggtg gaagaccctg
    13441 ctaatctggg agcaattatt agaacagctg ctttttttgg agtggaaggg attttaatta
    13501 gcaaagcccg ctctgctccc ttagactcac cagttgtgag agcctcagca ggagggatag
    13561 agagcataga tatagctcag attaataact ttactcaagt gataaaagaa tttcaggaag
    13621 caggtttctg gatagtagct ctggaagcag atggagataa agacatctgg gatgcagacc
    13681 tccggggtat gc
//
LOCUS       DAOSVE010000041        13667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_2694_length_13667_cov_63.338084, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000041 DAOSVE010000000
VERSION     DAOSVE010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 13667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13667
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2694_length_13667_cov_63.338084"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..1393
                     /locus_tag="PK016_04830"
     CDS             74..1393
                     /locus_tag="PK016_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HXL02429.1"
                     /translation="MRTTPFWDEYYELLPREELEKIQLSRLKKVLIRAYHNLPYYRKK
                     LEERKINPYDINSLSCLSHLPCTTKEDLRQAYPFGAIAGPLRNVVRIHSSSGTTGTAT
                     VVGYTKKDIEAWAELAARALSSYGVDEEDIIQVSFGYGLFTGGLGIHYGAEKLGATVV
                     PASAGNTLRQIQIMKDLKITALACTPSYALYLAEVAKENNVNWKESSLRIGVFGAEPW
                     SEEMREDIEKKLPIRAFDIYGLSEVIGPGVAFECEERKGLHISEDHFLPEIIDPQTGK
                     VLPPGEEGELVFTTLTKEGTPVIRYRTGDISSLNYEPCFCGRTLVRMKRVFSRTDDML
                     IIRGVNVYPSQIESVILSFDGVEPYYQIVLTRENFLDQIEVQVEVTPQFFSDQIKVLE
                     GLKKQIEEKLRTELNLSCEVKLLEPKSITRSEGKSKRVIDLRKKEEI"
     gene            1393..1809
                     /locus_tag="PK016_04835"
     CDS             1393..1809
                     /locus_tag="PK016_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HXL02430.1"
                     /translation="MKQISVFLENKPGSLYEVLYKLKEWGVNLRALSLADTAEFGVLR
                     FIVENPEEIVEKLKRENFAATLTEVLAVGVEDRPGGLAEVVGILTHQNISIEYLYAFV
                     SPQGGKAYVVLRVEKLEEAEKILRGNHIPILEKIVI"
     gene            complement(2096..3430)
                     /locus_tag="PK016_04840"
     CDS             complement(2096..3430)
                     /locus_tag="PK016_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018975612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HXL02431.1"
                     /translation="MLKGIKKHRKIIAVFVLATIVVFSFGMVVFAEQTQPKQMQPKET
                     FYRTLTKIYDWGAAVPEVIIDLGQTVKQGAVSKDTFSVLVSRTDPRVPTPLLESGYRT
                     VINAYVSDENGNPVEAGQYVTLEMEIGPNVSLGSPLNYYNGRNEWIRCDYVITQLKDI
                     PFDSGTISGLIANKFAGGIRELVDDFSIGKSTYNGITLNYAYYAPAKDDQKHPLIIWL
                     HGGGEGGVDATIPLAANKCVNLITEEIQSYFGGAHVLVPQAPTFWMDGFTGTGDGTSI
                     YEDALMSLIKDYVSQNSDIDANRIYIGGCSNGGYMTMIMIRDYPGYFAAAFPVCEPLH
                     DKLITNADLREIMKTPIWFTCAATDPVVLPAIYAVPTYNRLLTAGATNVHLSYFDKVI
                     DTTGLYKKEDGTPYEYNGHWSWIYVYNNECTTVIDGKIVSIMEWMAAQSLNK"
     gene            3603..4703
                     /locus_tag="PK016_04845"
     CDS             3603..4703
                     /locus_tag="PK016_04845"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HXL02432.1"
                     /translation="MRKKITIKDIAQELNVSVATVSRALNGEKGVNSDLRKKVIKKAQ
                     NMGYFRWSSLNLLNGEPKKIAVIFPENDYFWEKVDKGVQDAQELLQHSEIELKFFRTT
                     GHNLAQQNQLLENILQKEKIDGLALVPADFTQLDYFINALYLKGVAVATFNIDAPLSK
                     RLFYVGQNSNQAGRVTGKIFDYLLRGKEGKISILTSNKRAPSHMGRWQGLIDFVHKKG
                     LSLDVSQVVEAKDDEEAYKITKDKLLEEKDLLGIFVSTAFGNIGVGKALKEKGKKDLV
                     VIGNDLGEGWIPHIKEGIIDCCLFQAPYLQGYLALMLLAKFLLFEENPPDKNIFIPII
                     PVFSENLDGDFLNFEQCVTRHASLNYQFLDTD"
     gene            4876..5955
                     /locus_tag="PK016_04850"
     CDS             4876..5955
                     /locus_tag="PK016_04850"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HXL02433.1"
                     /translation="MAFAGEVEKSFWGTTKEGAPIDLYTLTNDNGVMVQLTNYGATVV
                     SLYVPDKEGNLGDVVLGFDSVSDYEKPDPYNPYFGATIGRYGNRIAGGQFTLDGKTYT
                     LAQNNGPNHLHGGLKGFKDQIFEGIPMKTPEGPAVRFKYLSHDGEEGYPGNLQVSVTF
                     TLTNDNELKIKYLATTDKPTVVNLTNHSYFNLAGEGSGNILDHELMIVADNYTPADDT
                     LIPTGIAPVAGTPLDFTAPHKIGERIEQLLRKEGDDPFKGYDHNFVLNNQTGELALAA
                     RAKEATTGRVMEVYTTEPGVQLYTGNWVNTPGKEGKLYGQYSGFCLETQHYPDSPNHP
                     DFPSTVLRPGEVYSTLTIYKFLVEE"
     gene            6016..7104
                     /locus_tag="PK016_04855"
     CDS             6016..7104
                     /locus_tag="PK016_04855"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXL02434.1"
                     /translation="MNSKERVKIALEHREPDRVPVTASFTPEFAQRLRDYFSLPSQFS
                     NPHGGEVHDLEEILGLDIIQYAVGIGNSFYASEEKEYICDWGIKWRQVEYETPFGRGH
                     YTEIAEHPLTGDGLYRYVSPDPHREELYAPLEGVMEKYGEKYFILGVTVCTIFEASWY
                     LRGMSQLLMDMVEDEEKANYVLDIPFHFHLETAKKLVKMGVDGIWLGDDVGSQKGMLI
                     SPELWRKYLKPRMATICRELKNINPHLKIAYHSDGNIYPIIPELIEIGIDVLNPVQPQ
                     AMDPAFLKERYGDKLSFWGTIDEQYTLPFGTKEEVERETKERIKVMAPGGGFIISPTH
                     HVQLDTPLENFFTFLETVKKYGCYPLSF"
     gene            7198..8190
                     /locus_tag="PK016_04860"
     CDS             7198..8190
                     /locus_tag="PK016_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HXL02435.1"
                     /translation="MKKTHWLVIFLVISVLVLSSSFAFAQGKRFEGIKIRFFCGGAPG
                     DPFASVVYKGAQFAQEDLGCNVEYVFSGWDPQQMVVQFKEAVASDVDGIAIMGHPGDD
                     ALETHIDEAFSKGIIVTSQNTTLPRAEEKYKARGFGYVGQELYESGVTLGKGCVSMFD
                     LKPGDRAMVWGLLSQPTRGLRTKGVIDALEEAGLTVDYIEISPEVNADAPLGVPIFTG
                     YVSSRPDVKLVVTDHGSLTATMQTFFQAAGLGPDDVVGAGFDLSSATLEAIRSGYTDL
                     VLDQQQFLQGYLPVLQLCMSIKYKFSGLHIDTGSGLVHAGNVEEIAPLVEEGIR"
     gene            8191..9021
                     /locus_tag="PK016_04865"
     CDS             8191..9021
                     /locus_tag="PK016_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXL02436.1"
                     /translation="MAEPSEVLVKMANICKSFGHVEVLRNVNFEVGYNEVVGLLGDNG
                     AGKSTLIKILTGVHRPDRGEIYWKGQKLEDYSVAKARDLGVETVFQERALCEKHAIWR
                     NIFMGREITKLGGFVDSQKERDETEKIMKELMGFTSSALSVDSEVGDLSGGEKQGVAI
                     ARALYFQADLIILDEPTTGLSLSETQKVLNFVEDIKRQGKSAIFITHNIYHVYPVADR
                     IVVLDRGRAVGEFEKAGVSLEELVNKLYLVAQTGSLNNQGAVSSPAGSGKDDLQSLMP
                     "
     gene            9130..10092
                     /locus_tag="PK016_04870"
     CDS             9130..10092
                     /locus_tag="PK016_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL02437.1"
                     /translation="MKKEQSIFSQHQLQISVLLVFFGVLVLFIIGSPQTFLSSNIYYA
                     FMSNIPFFGILALSLTLVVICGDMDLSFPSIMGFAGYIFTTSFHATQSVGLALLFSLL
                     VGLGAGLMNGLIIVKLHLPSLVVTIGTQFFWAGATQVLSGGLGKTLVPTKASSLYPVF
                     VGRIGGKLPAQMLWMVAIAFIIWLILNHHRFGAHIYFTGDNPHSARVMGVNVDRVRML
                     VFTQMGIFSAFAGILASLEVVYFWPTLGQGYMLKTMAAVFLGGTPVTGGVGTIFGTFM
                     GIIIIGMLEAGIIAMGMTGFWTQLVYGLIIVVSVAMYSIMGRRK"
     gene            complement(10082..10420)
                     /gene="rnpB"
                     /locus_tag="PK016_04875"
     ncRNA           complement(10082..10420)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK016_04875"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(10414..10860)
                     /locus_tag="PK016_04880"
     CDS             complement(10414..10860)
                     /locus_tag="PK016_04880"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HXL02438.1"
                     /translation="MVDFFVENKEFIAFIDGASKGNPGESSYAFVLGDGEGNIIMEAA
                     VRIGIATNNEAEYRALLAVLDQVLEEGGEALRVFSDSQLLVKQINRLYRVKSPNLIPL
                     YQEAQSKIQKLGYFEIFHIPREENQKADHLASEILTQKLSFKVWRK"
     gene            complement(10835..11539)
                     /locus_tag="PK016_04885"
     CDS             complement(10835..11539)
                     /locus_tag="PK016_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HXL02439.1"
                     /translation="MSEILEILLALQDVDLKIDSGKKRIVKKEEEIADINKQLENLKA
                     NLEEKKHNFEKARLKLAQEELELKELEERLKTTKNKLYSGEISSPKELSQWEKTIEKL
                     EESKNSLENNVITSLENAEELQKEIQEKNQSYSQKEAIFLSLLHKLQGELEAEKISIS
                     EKEKEREKIIASLPEEIISTYEELRKKFSDPVTYLNGETCEGCNLTVPLAIIKAVRKG
                     ESLVRCPNCGRFLRRK"
     gene            complement(11532..12632)
                     /locus_tag="PK016_04890"
     CDS             complement(11532..12632)
                     /locus_tag="PK016_04890"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HXL02440.1"
                     /translation="MERLETVVSFWETLFPSSLAFPSDPPSLQIKGEDRVEKILVALE
                     LNPRVFRQAIKENYDFLYLHHPPIWKPLTSFSSDDPWLSMLGELYYRKISILAHHTNL
                     DSAKGGLADQWIKILSLEGKTKPLEPHSSRGFKVVTFVPLSHREKVLEAIFSSGGGKI
                     GNYEECGFLLSGKGTFRPQEGARPFWGKIGEREEVEEIRIEVNVEEHFLSRVIESIFS
                     SHPYEQPVIDVYPLFSSGDTGLGRVITLSSNISREDLERRISLAFSLVAHYPDDDFPV
                     VYQKIALCPGSGKSLLDRVIKEKPQVFLSGDLSHHDIAELNLWGVTYYSIPHEEGERK
                     ALKEVYQDLKEKFKEQGLKVELNFETEGKNNE"
     gene            complement(12653..12727)
                     /locus_tag="PK016_04895"
     tRNA            complement(12653..12727)
                     /locus_tag="PK016_04895"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12693..12695),aa:Ile,seq:cat)
     gene            12795..12950
                     /locus_tag="PK016_04900"
     CDS             12795..12950
                     /locus_tag="PK016_04900"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HXL02441.1"
                     /translation="MTDFTVNYTQNLANMAKFRNLLVHLYWKVDDERLYQILQSQLPY
                     LTLLLTV"
     gene            complement(13074..13262)
                     /locus_tag="PK016_04905"
     CDS             complement(13074..13262)
                     /locus_tag="PK016_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02442.1"
                     /translation="MINWRFPSEYRSRTVERCASSLPKIHSEDEALFLAETLGIEVVF
                     VRLEEAKGGLLCICARYF"
     gene            complement(13511..13587)
                     /locus_tag="PK016_04910"
     tRNA            complement(13511..13587)
                     /locus_tag="PK016_04910"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13551..13553),aa:Arg,seq:tcg)
BASE COUNT         4035 a         2591 c         2876 g         4165 t
ORIGIN      
        1 atcacaccat atcctttctc gttggcctat ccttaaaata ccgataaagt ggtaaaatat
       61 aaactaaaaa gaggtgagaa caacgccatt ctgggacgaa tattatgagc tcctcccccg
      121 ggaagagctg gaaaaaattc agcttagcag acttaaaaaa gtactcattc gcgcttatca
      181 caacctgcct tattatcgca aaaaattaga ggaaagaaaa attaatcctt acgatatcaa
      241 ttctctatcc tgtctttctc atctgccttg caccactaaa gaagacctcc gccaagctta
      301 tccttttggg gccatagcgg gacccttacg taacgtagtg cgaattcatt cttcttcggg
      361 aaccacagga acagctacgg tagtggggta taccaaaaaa gatatagagg cctgggcaga
      421 gctcgctgct cgagcgcttt ccagctatgg agtagatgaa gaagatatca tccaggtctc
      481 ttttggatat ggtcttttta ctggaggttt aggaattcac tatggagcag aaaaactggg
      541 tgctacagta gtgcctgctt ctgctggtaa tactcttcgc cagattcaga ttatgaaaga
      601 cctgaaaata accgcgctag cctgtacccc ctcttatgcc ctctacttag ctgaagtagc
      661 caaagaaaac aacgttaact ggaaagaaag cagcttgcgt attggggtat ttggagctga
      721 accctggtca gaagaaatgc gagaagacat agaaaaaaaa ctacctattc gagcttttga
      781 tatctatggc ttgagtgaag ttatagggcc aggagtagct tttgagtgtg aagaaagaaa
      841 aggattacac atctctgaag accacttcct tcccgagata atcgaccccc agacagggaa
      901 agttttacct cccggagaag aaggagagtt agtatttacc actcttacta aggaaggcac
      961 cccagtaatt cgttatcgca ccggagacat atcttctctc aattatgagc cttgtttttg
     1021 tggaagaaca ctggtaagga tgaaaagagt tttttctcgc accgatgata tgctcattat
     1081 tagaggagta aatgtttatc cttctcagat tgagagcgtc attttaagct ttgacggagt
     1141 agagccctat tatcagatcg tcctcacccg agaaaacttt ttagaccaaa ttgaggttca
     1201 agtagaagtt acacctcaat tcttttctga ccagataaaa gtattagaag gattaaaaaa
     1261 acaaattgaa gaaaagctga gaactgaatt aaatttaagt tgcgaggtaa aactcttaga
     1321 gcccaaaagt attacccgca gcgagggaaa atctaaaagg gttatagacc tgcgaaaaaa
     1381 ggaggagatt tagtgaaaca aatatcagtg tttttagaaa ataagccagg tagtctgtat
     1441 gaagtgcttt acaaattgaa ggaatgggga gtaaacctgc gagcactttc tctcgctgat
     1501 actgctgagt ttggagtttt aagatttatc gtagaaaacc cggaagaaat agtagaaaaa
     1561 ttgaaaaggg agaatttcgc tgctacttta actgaagttc tagctgtagg agtagaagac
     1621 cgaccgggag ggttagccga agtagtaggc attttaactc accaaaatat ctccattgaa
     1681 tatctctatg ccttcgtttc tccccagggg ggaaaagcat atgtagtgtt gcgagtagaa
     1741 aagttagaag aagctgaaaa aatattgagg ggaaaccata tccctatttt agagaaaatt
     1801 gtaatttaaa agcaatccgg agatagggga agaataaata ccttcatcta tctccctttc
     1861 tattatcgcg agggctacaa ataacagttt aggcataaga ttaaaagcat aatgatgaaa
     1921 aaacttcggg attttaacgg gataagctaa cccagctaca tggtgatcca ctttttagcc
     1981 ctgaaaaaag ctttaaaagg tgaccttcgg caagttcagg gaattgaaga taaaaaaggc
     2041 tgtagaaaat tgcttaaaca tgatcttcta cagccctgga aaaccatata actaattatt
     2101 tattcagtga ctgagcagcc atccattcca tgattgatac tattttgcca tcaatgacgg
     2161 ttgtacactc attattgtat acataaatcc atgaccagtg tccattgtat tcatatggcg
     2221 taccatcttc ttttttgtat aaacccgtag tatcaattac tttatcaaaa tatgacagat
     2281 gaacattggt tgctccagct gtaagcaatc ggttataagt aggaacggca tagattgctg
     2341 gaagtactac cggatctgta gctgcacagg tgaaccaaat gggagttttc ataatttcac
     2401 gaaggtctgc gttggtaatt aatttatcat gaagaggttc gcacaccggg aaggcagctg
     2461 caaagtaccc tggatagtca cgaatcataa tcatggtcat atatccacca ttagagcatc
     2521 caccaatgta tattctattg gcatcaatgt cactattctg ggaaacataa tccttgatta
     2581 atgacatgag tgcatcctca tatattgatg tcccatctcc tgttcccgta aaaccatcca
     2641 tccagaatgt aggcgcctga ggcactaaaa catgagctcc accaaaatag gattgaattt
     2701 cttctgtgat caggtttaca catttatttg ctgcaagagg aatagttgca tctaccccgc
     2761 cttctccacc accgtgtaac catatgatta aggggtgctt ttgatcatct tttgcaggtg
     2821 catagtatgc atagtttaga gtaataccat tgtatgttga ttttccaatt gaaaagtcat
     2881 ccacaagttc tcttattcca cccgcaaatt tattagcaat cagacctgaa attgttcctg
     2941 aatcaaaagg aatatccttt aactgagtaa tcacgtaatc gcatcgaatc cactcgtttc
     3001 tcccgttata ataattgagc ggtgagccca acgatacatt cgggccgatt tccatttcta
     3061 atgtaacata ctgtccagcc tcaactggat ttccattttc atcagacaca taagcattta
     3121 tcactgtacg atatccactt tccaacaacg gagtaggcac tctaggatca gttctggata
     3181 ccaataccga gaaagtatct ttactcacag ctccttgttt aacagtttga cctagatcga
     3241 taattacttc tggaactgct gccccccaat cataaatctt tgtaagggta cgataaaatg
     3301 tttcttttgg ttgcatttgc ttaggctgcg tttgctcggc aaaaacaacc attccaaaag
     3361 aaaaaacaac aattgtcgcc aaaacgaata ccgcaataat tttcctatgc tttttaatac
     3421 cttttaacat tttatcttac ctcctttaat aataatttgt gcatttacga cataattata
     3481 atatctgagt ataaattaaa tcaaactttt ttcaaatttc tgcttattat agagccctaa
     3541 aaaatttgac aaatttctga gataatgtta tctttagtaa aaatttatct gaaggttata
     3601 aaatgaggaa aaaaattacg attaaagata tagctcaaga actgaatgtt tcagtagcta
     3661 ctgtgagtcg ggcgttgaac ggtgaaaaag gagttaactc tgatttacgg aaaaaggtta
     3721 taaaaaaggc tcaaaatatg ggatatttcc gctggtcttc tcttaatcta ttaaatggag
     3781 agcctaaaaa aatagcagtt attttcccag aaaatgacta tttctgggaa aaagttgata
     3841 aaggtgtcca agatgcccag gaattattac aacattctga gatagaactg aaattttttc
     3901 gcaccaccgg tcataactta gctcaacaaa accagttgct ggaaaatatc ctgcaaaaag
     3961 aaaaaataga tggcttagct ctcgtccctg ccgattttac tcagttagat tattttatta
     4021 atgctttata tcttaaaggt gtagcagtag ccacttttaa catagatgca cccttaagta
     4081 agagactttt ctatgtagga caaaactcaa atcaagcagg tagagtaaca gggaagatat
     4141 ttgattatct cttgaggggt aaagagggga aaattagtat tttaactagc aacaaaagag
     4201 ctccttctca tatggggcga tggcaaggac taatcgattt tgttcacaaa aaaggccttt
     4261 ctttagatgt ttctcaagtg gtagaagcca aagacgatga agaagcttac aagattacta
     4321 aagataagct cctggaagaa aaagatttgc tgggcatttt tgtttctact gcttttggta
     4381 acataggagt gggaaaagct ttaaaagaaa aaggaaagaa agatttggtg gtaataggca
     4441 acgatttggg ggaaggatgg atccctcata ttaaagaggg aatcatcgat tgttgccttt
     4501 tccaggctcc ttatttgcag ggatatttag ctctaatgct attagctaag tttttgctgt
     4561 ttgaagaaaa ccctcccgat aaaaatattt ttattcccat tattcccgtt ttttccgaaa
     4621 acttagatgg agactttttg aattttgaac aatgtgtaac aagacacgct tctctaaatt
     4681 atcagttctt agacacagac taagggagga gttttttatc atctattact gaccacaagg
     4741 aggtgagtaa aggggaatta attttatttg gttcttaacc ttcacaggat taaaacattt
     4801 taaaggagga atgtagtcgt gaagaagata gccagtttgt taatagtttt gttactggtg
     4861 tggggggtta gtgccttggc ttttgctgga gaagtagaaa aatctttctg gggaactact
     4921 aaagaaggag caccaataga cctttatact ctcacaaatg ataatggagt tatggtgcaa
     4981 ttaaccaatt atggggctac agtagtgtct ctttatgtcc cggataaaga aggtaactta
     5041 ggcgacgtgg tattaggttt cgatagtgta tccgactatg aaaaacctga tccttacaat
     5101 ccttattttg gtgccacaat aggacggtat ggtaatagaa tcgccggtgg gcaattcact
     5161 ctggacggaa aaacttatac cctggcgcag aataatggcc ccaaccattt gcacggagga
     5221 ttaaaaggat ttaaggacca gatatttgaa ggtattccta tgaaaactcc tgaaggccca
     5281 gcagtccggt ttaaatattt gagccacgat ggagaagagg gatatcccgg aaatctgcaa
     5341 gtgagcgtta cttttacatt aactaacgat aacgaattga aaataaaata tttggctacc
     5401 accgataaac ctacagtagt taatttaacc aatcatagtt acttcaacct tgccggagaa
     5461 ggttcaggca atatcttgga ccacgagttg atgattgtgg ctgataacta tacccctgcc
     5521 gatgataccc ttattcctac gggaattgct ccggtagcgg gaactcctct ggattttacc
     5581 gctcctcata aaataggaga gcgaattgag caattgcttc gtaaagaagg agacgatcct
     5641 tttaaaggtt atgaccacaa ctttgtgctc aataaccaga ctggagagtt agctttagct
     5701 gctcgggcta aagaagctac taccggccgg gtaatggaag tttatactac tgagcctgga
     5761 gttcagcttt acactggaaa ttgggtgaac actccaggga aagagggaaa actctatggg
     5821 cagtattctg gtttttgctt agagactcaa cactatcccg attctcccaa ccatcctgat
     5881 ttcccctcta cagttttaag accgggtgaa gtttattcta cattaactat ttacaagttt
     5941 ttggtagaag agtagattag ttagattcct cccctgtttc cgaaacaggg gaggaatgaa
     6001 tggggagagg tagcaatgaa ctctaaagaa agagtaaaaa ttgctcttga gcaccgtgaa
     6061 ccagaccgag taccagttac cgctagcttt actcctgaat ttgcccaacg cttgcgagat
     6121 tatttcagct taccctctca gttttccaat cctcatggag gagaagtcca cgatttagaa
     6181 gaaatcctgg ggctggatat aatccagtat gcggtaggaa taggtaattc tttctatgcc
     6241 tcagaggaaa aagaatacat ttgtgattgg gggataaaat ggcggcaagt agaatacgag
     6301 acccctttcg ggaggggaca ttatactgaa atagctgaac atcccttaac cggcgacggg
     6361 ctctaccggt atgtttctcc tgaccctcac cgagaggagc tttatgctcc tttagaagga
     6421 gtaatggaaa aatatggtga aaagtatttc atcttaggag ttacagtatg taccattttt
     6481 gaagcttcct ggtatttgcg aggaatgagc caattgctta tggatatggt ggaagatgaa
     6541 gaaaaagcta attatgttct ggacattcct tttcattttc acttagagac agctaaaaag
     6601 ttggtgaaaa tgggagtaga tggcatctgg ttgggagacg atgtaggatc acagaaagga
     6661 atgttaattt ctccagagct atggcgaaaa tacttgaaac cccgaatggc taccatctgc
     6721 cgggaattaa aaaatatcaa ccctcatcta aaaattgctt accatagcga tgggaacatt
     6781 tatcctatta ttcccgaact aatagaaata gggatagatg tcctcaatcc ggtgcaacct
     6841 caagctatgg atccggcttt tttaaaagag cgttacggag ataaactctc tttctgggga
     6901 actattgatg agcaatatac tcttcctttt gggactaaag aagaggtgga gagagaaaca
     6961 aaggagagga taaaagtaat ggctccggga ggtggtttta ttatttctcc tactcatcat
     7021 gtacagttag atacccctct cgagaatttc tttacttttt tagaaacagt taaaaaatat
     7081 ggctgttatc ccctcagctt ttagagtaaa ataagcagag aaggaggtga gattttagtt
     7141 tttgggggtt aaaagccatt ttaagtagtt tatcaaattt aaaaggaggt ttaactcatg
     7201 aaaaaaactc attggctagt tatatttttg gtaataagtg tgttggtgtt aagtagtagt
     7261 tttgcttttg cccagggtaa gagatttgaa gggattaaaa tacgtttctt ctgtggtggt
     7321 gctccgggag acccctttgc cagcgtagtt tataagggag cacaatttgc ccaagaagat
     7381 ttaggatgta acgtagaata tgtcttttct ggttgggatc ctcaacaaat ggtggttcag
     7441 tttaaagaag cagtagcttc tgatgtagat ggtattgcta ttatgggaca tcccggtgat
     7501 gatgctctgg aaactcatat cgacgaggct tttagtaaag gtatcatagt gacctctcaa
     7561 aacaccaccc ttccccgggc ggaagaaaag tataaagctc gcggatttgg ctatgtagga
     7621 caggaacttt atgaatcagg agtcaccttg ggtaaaggat gcgtaagtat gtttgattta
     7681 aaacctggag atcgagctat ggtttgggga ttgctttctc agcctactcg aggattaaga
     7741 actaaaggag tcatagatgc cttggaagaa gcaggactga cagtagatta tatagaaatt
     7801 tctcctgagg ttaacgctga cgctcctttg ggcgttccta tttttactgg ctatgtttct
     7861 tctcgccccg atgtaaaatt agtggttact gaccatggtt ctttaactgc tactatgcaa
     7921 actttcttcc aggctgccgg tttagggcca gatgatgtgg taggagctgg ttttgacctc
     7981 tcatctgcta ctttagaagc tatccgcagc ggatatactg atttagtttt ggatcagcag
     8041 cagttcttgc aaggctatct tcctgttctc cagttatgta tgagcattaa gtataaattc
     8101 tccggacttc acattgatac cggatccggt ttggttcacg ctgggaatgt ggaagaaatt
     8161 gctcctttgg tagaggaggg aatcagataa ttggctgaac catctgaagt tctggttaaa
     8221 atggctaata tttgcaaatc ctttgggcat gtagaggtat tgcgaaatgt taactttgaa
     8281 gtaggttaca acgaagtagt gggtcttttg ggagacaatg gtgcaggaaa atctactttg
     8341 attaagattt tgaccggggt tcaccgtcct gatcggggag aaatttactg gaaagggcag
     8401 aaattagagg actattcagt agccaaagct cgagacttgg gggttgaaac tgtctttcaa
     8461 gaaagggcac tatgcgagaa gcatgctata tggagaaata tatttatggg aagagaaata
     8521 actaaattgg gaggctttgt tgatagtcaa aaagaaagag atgaaacaga aaaaattatg
     8581 aaagaactta tgggttttac ttcttctgct ctttcggtgg attctgaggt tggagacctc
     8641 tcgggaggag aaaagcaagg ggtagccatt gctcgagctt tgtatttcca ggctgacctc
     8701 atcatcctcg atgaacccac cactggtctt tctctttctg agacccaaaa agtgttaaac
     8761 tttgtggaag atataaaaag acaaggaaaa tctgctatat ttattactca caatatctat
     8821 catgtttatc cggtagctga ccgtatagta gttttagacc gaggtagagc ggtgggtgag
     8881 ttcgaaaaag caggggtttc tctagaagaa ttagtaaata aattatatct ggtggctcag
     8941 acgggaagcc ttaacaatca gggagctgta tcttcaccag ctgggtcagg aaaagatgat
     9001 cttcaatctc tcatgcctta aaagaatttg aacctttaag ctaggacaat attgatgtag
     9061 gagtagagta gaatcattag aggattcttc tctactccta aaactaggat tagagtaggg
     9121 ggactctgga tgaaaaagga gcaaagtata ttttctcagc atcagttaca aattagtgtt
     9181 ctgttagttt tttttggggt tttagttctc tttattattg gtagtccaca gacttttctt
     9241 tctagtaata tttactatgc ttttatgtcc aatatcccct tttttggtat tttagcactc
     9301 tccttgacgc tggtcgtaat atgcggggat atggatcttt cttttccctc cattatggga
     9361 tttgctggat atatatttac tacgtctttt catgccactc aaagtgtagg attagcttta
     9421 ctattttctc tcttagtagg tttaggagcc ggcctgatga atggtttaat aatagtgaag
     9481 ctacaccttc cttctttggt ggtgaccatt ggtactcaat tcttttgggc tggtgctact
     9541 caagttttaa gcggaggatt agggaaaacc ctggttccta ctaaagctag ttccttatac
     9601 ccagtgtttg tgggtagaat tggagggaaa ctaccagctc aaatgctttg gatggtagca
     9661 attgctttta taatttggtt aattttgaat catcaccgct ttggagctca tatttatttt
     9721 actggtgaca atcctcatag tgctcgggtt atgggagtta atgtagaccg ggtgagaatg
     9781 ttagtcttta ctcaaatggg tattttttct gcttttgctg ggatattagc cagtttggaa
     9841 gtggtttact tctggcccac cctgggacag ggatatatgt taaaaactat ggcagctgta
     9901 tttttaggag gaactccagt aactggagga gtgggaacga tttttggtac ttttatgggc
     9961 attataatta ttggtatgtt ggaagcaggc attattgcta tggggatgac tggattctgg
    10021 actcagctgg tgtatggttt aataattgta gtatctgtag ctatgtattc gattatgggg
    10081 aggagaaagt aggcctataa gccgagttct gtattcccgt aggaacgaca accatctatc
    10141 tggggggtta gttgcctaac cccctcgagc ggccaacccg aaggtcagcg agccacctca
    10201 tcccttccta tttggccttg ctccgggtag ggtttgccag gcacgccgat tgctcgacgt
    10261 ccggtgagct cttacctcgc cttttcaccc ttacctttta tggcggtatc ttttctgtgg
    10321 cactttccgg gagttacctc ccctgggtgt tacccagtac cctgctctgt gaagctcgga
    10381 ctttcctcag gatttctccc gcggttgtcc agcctacttt ctccatactt taaaactcaa
    10441 tttttgagtt aatatttccg aagctaaatg gtcagccttc tgattttctt ctcgaggtat
    10501 gtgaaaaatc tcaaaataac cgagtttttg gattttactc tgtgcttctt ggtagagagg
    10561 aattaaatta ggagacttta cccgatacag acggtttatc tgctttacga gaagctggct
    10621 gtcagagaaa acccggagtg cttctcctcc ctcttctaaa acctggtcta gcactgcaag
    10681 taaagctcga tattctgctt cattgttagt ggcaataccg attcggacgg cagcttccat
    10741 aattatattt ccttctccat ctcctaaaac aaaagcataa gaactttctc cggggtttcc
    10801 tttcgatgct ccatctatga aagcaatgaa ctctttattt tcgacgaaga aatcgaccac
    10861 agttgggaca ccgcaccagc gattctccct tacggacagc ttttattata gctaagggaa
    10921 cagttaagtt acatccttca caggtttctc catttaaata agtgaccggg tcagagaatt
    10981 ttttcctcaa ttcttcatag gtagaaatta tttcctcagg taaagaagcg attatttttt
    11041 ctctttcctt ttctttttcc gaaattgaaa ttttttctgc ttctaattct ccttgcaact
    11101 tgtggagtag agagagaaag attgcctctt tctgggaata agattggttt ttctcctgga
    11161 tttccttctg caattcttca gcattttcta aagaggtgat aacattattt tctaaggaat
    11221 ttttgctttc ttctaatttt tctatagttt tttcccactg agataattct ttaggagagc
    11281 taatttcccc gctatagagt ttgtttttag tagttttcaa tctctcttcc agctctttca
    11341 gctccagttc ttcttgagcc aatttgaggc gtgctttttc aaaattgtgt tttttctctt
    11401 ctaagttagc ttttaagttt tctaattgtt tattaatatc ggctatttct tcttcttttt
    11461 ttactattct ctttttcccg ctgtctattt taaggtcaac atcttgtaaa gccagaagaa
    11521 tttccaaaat ttcactcatt attttttccc tcggtttcaa aatttaactc tacttttaat
    11581 ccttgttctt taaatttttc ttttaaatct tgatacactt cttttagagc ttttctctct
    11641 ccttcctcat gaggtatgga gtaataagtt actccccaca ggtttagctc agctatatca
    11701 tgatgggaga ggtctccact caagaatacc tggggttttt cttttattac tctatctaaa
    11761 aggcttttcc cacttccagg acaaagagct attttttggt aaacaacagg aaaatcatcg
    11821 tcaggatagt gggctaccag agaaaaggca agagagattc tcctttctaa atcttctcgg
    11881 gaaatattcg aagatagagt aattactctc cctaaacccg tatctccaga tgagaaaagg
    11941 ggataaacat caataactgg ttgttcatag ggatgagaag aaaaaatgct ttctattacc
    12001 cgagataaaa aatgctcttc cacattgact tctattctaa tttcttctac ttcttctctt
    12061 tctcctattt ttccccaaaa aggacgagct ccttcttgag gtcgaaaagt ccccttccca
    12121 gagagaagaa aaccacattc ttcgtaattg cctatttttc ctcctcccga ggagaaaata
    12181 gcttccagaa ctttctctcg gtgagaaaga ggaacaaaag taaccacttt aaatcctcga
    12241 gaggaatgag gttcaagagg ttttgttttt ccctccagag acagaatctt tatccactgg
    12301 tcggccaggc cacctttggc actatctaaa ttggtatgat gagcaagaat agatattttt
    12361 cggtaataaa gttctcctaa catggatagc cacgggtcat cgctggaaaa agaagtaagg
    12421 ggcttccaga tagggggatg gtgaagatag agaaagtcat agttttcttt tatcgcctgg
    12481 cggaataccc gaggatttaa ctctaaagcc accagtattt tttctacccg gtcttctcct
    12541 ttgatttgca aagaaggagg atcagaaggg aaagcaagag aagaaggaaa aagtgtttcc
    12601 cagaaactaa ctacagtttc taatctttcc atcttttctc ccttagaata aatggtgggc
    12661 ccacccggat tcgaaccagg gaccgaccgg ttatgagccg gctgctctac cgctgagcta
    12721 tgggccccaa aaacagaata aatattacca cattcttgtt ttcttatcaa gaaggctctc
    12781 gataacggtt aattataact gattttacag ttaactatac gcagaactta gccaatatgg
    12841 ccaaattcag aaatttgctg gtccatcttt actggaaagt tgacgatgaa aggctctatc
    12901 agatattgca atcccaactc ccatatttga cgcttttatt gactgtatag ctaaaaaata
    12961 cctttaggtt atttccctac tctttcataa ttcttcctcc aaccactccc gcatcaagag
    13021 gaaagcgaag tagtcggctt ctttttctaa gccggcgata aagctctggt gatctaaaaa
    13081 taccgggcgc agatacagag taatcctcct ttagcctcct ctaagcgaac aaatacaact
    13141 tctatcccta acgtttcagc gagaaatagc gcctcgtctt cagaatgtat tttaggtaaa
    13201 gaagaagcgc agcgctccac agtccggctt cggtattcgg agggaaagcg ccagttaatc
    13261 attctttttt ttctttctcc ctttcaagca ttgcaaagag aggcactaaa taaattagta
    13321 agacatcttc tgggaaaata gtaaagcaag aggggctctt tttgctctcg caaattgata
    13381 aagataaaat aaagctcaaa gatattgaag gatataatga cgttgcagta agatttgact
    13441 gagagaaata aatgaggccg tcgactataa cggagttttt tagagagaga aacgagcaaa
    13501 tgcctttttc tggcgcgcct gaagggagtt gaacccccaa cctacggatc cgaagtccgc
    13561 cgctctatcc tattgagcta caggcgcgca cataaaagct ttactttagc tgcttctatt
    13621 ttatatttta catctttttc tgtcaatgaa catccagctt ctgagag
//
LOCUS       DAOSVE010000042        13517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_2727_length_13517_cov_72.202942, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000042 DAOSVE010000000
VERSION     DAOSVE010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 13517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13517
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2727_length_13517_cov_72.202942"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="PK016_04915"
     CDS             complement(<1..977)
                     /locus_tag="PK016_04915"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXL02443.1"
                     /translation="MNKLLWITIILALSFLVITPVMGEEKVRLSSEELLELSKSFFQY
                     LRTGKYHLAEELFIPEVQEALPEEALKETWENLTSDLGPLKEMKDHQVAVEGGYQVVY
                     ARCEFEKAELKAKLVFSEEGKIIGFQLLPSSQIEYSLPDYVEEDKIIEREVVFGIPGW
                     ELPGTLTLPKGEGPFPGLVLIHGSGSNDRDETLLANKPLKDLAWGLASQGIAVLRYDK
                     RTYVHGNKIEDLSQFTVEEEVIEDALAALEFLRHQEEINPQQVFILGHSLGGQLVPEI
                     ARRDGKVAGVIILAGPTRPLSELVVEQMEYILSLDGQIDEKEKKQLEEI"
     gene            1136..1211
                     /locus_tag="PK016_04920"
     tRNA            1136..1211
                     /locus_tag="PK016_04920"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1169..1171,aa:Gly,seq:ccc)
     gene            1430..1834
                     /locus_tag="PK016_04925"
     CDS             1430..1834
                     /locus_tag="PK016_04925"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HXL02444.1"
                     /translation="MKRQKHVLMLLHLGLFLLIFSVLIPATAVFSSTSLGQSYLLLTV
                     DLEKELVVIKNVSDKTIDLTGYVLVSVKGNQQFTFPAIALKPGETVTVTSGKNAQSDP
                     PRYLLWTKQNIWNNNGDPAKLLAPDGKLVVAY"
     gene            2217..2669
                     /locus_tag="PK016_04930"
     CDS             2217..2669
                     /locus_tag="PK016_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HXL02445.1"
                     /translation="MIKIRFSTRSRYGLRFMIELATYYGKKDLVFLKEIAEKEGISEK
                     YLTQLAIPLKARGLISSRRGAGGGYRLSRHPAQITAKEVIETLEGGIFPVDCLESPDI
                     CSRASFCAARKMWKELQDKISETLEKFTLENLAQMSTNNSHSPIDYSI"
     gene            2678..3649
                     /locus_tag="PK016_04935"
     CDS             2678..3649
                     /locus_tag="PK016_04935"
                     /inference="COORDINATES: protein motif:HMM:NF014610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HXL02446.1"
                     /translation="MKAIGLISGGLDSALAVFLLKKQGIEVIGLNFHSPFWESKNTLR
                     QLAQDLEIALRAVEVREDYLELVRNPQFGYGKNLNPCIDCKIWMLREAKKEMEKEGAS
                     FIFTGEVVGQRPKSQLKNTLRLIERQSGLEGYLLRPLSAKLLPPTIPEEKGWVKREEL
                     LDLQGRGRKRQLELAEKWGIKHFSPPAGGCLLTDPNFSRRLKDLWESEEAYDFESVEL
                     LKVGRHFRLGASFKLVVGRNQAENEKLKEFLREGDILFFPREGKGPLALGRGKVDESL
                     LQEAASLFAYYIRSHGEAVSVGVKFDSFGEVIEIERMSEEEVKKRMI"
     gene            3660..4571
                     /gene="cysK"
                     /locus_tag="PK016_04940"
     CDS             3660..4571
                     /gene="cysK"
                     /locus_tag="PK016_04940"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HXL02447.1"
                     /translation="MRIKDDITQLVGNTPLVKLGKIGKNLPGELIAKLEFFNPCGSVK
                     DRIAVAMIEEAEKQGFLKAGSLVVEPTSGNTGIGLAFVCAERGYHLLLTMPESVSVER
                     RKILTRLGAEVVLTPSSEGMRGAIEEAKKIAGNTPRAFMPQQFENPANPRAHRETTAR
                     EIWEDTDGKVDIVVAGVGSGGTITGIAQALKEKKPAFQAVAVEPLRSPVLSGGSPGQH
                     SIQGIGAGFIPPVLKLELLDEVIAVSDEDAFLMSERLAKEEGILAGISSGAALWGAVQ
                     VATREENKEKLIVVIIPDTGERYLSLW"
     gene            4577..5626
                     /gene="mnmA"
                     /locus_tag="PK016_04945"
     CDS             4577..5626
                     /gene="mnmA"
                     /locus_tag="PK016_04945"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HXL02448.1"
                     /translation="MRKKVLVAMSGGVDSSLSCVLLLERGFEVAGVTMCLGKKGKESA
                     CLVDVAVEDARQVCRTLSIPHYVVDFSREFEELVVEDFVSQYLKGKTPNPCVVCNQRL
                     KFGKLLGYARDQGFDFLATGHYAKIEEKEGKYFLKRPKDRRKDQTYFLYGIGRSNLPY
                     IIFPLADFTKEEVRKLSLKYNLSIAEKSDSEDICFLPEGDYRKFLEGRVSLDSPGFIV
                     TTEGKIVGEHKGYFRYTIGQRKGLGVAHPRPLYVVAVHPERNEVVVGEREVLQAQGLI
                     ASHFHWLVDELPSSVFAQVRYLQREKPCSVSIINSEKVQVIFKEKVEMISPGQSVVLY
                     SDDTVLGGGIIEEAF"
     gene            5650..6558
                     /gene="nadA"
                     /locus_tag="PK016_04950"
     CDS             5650..6558
                     /gene="nadA"
                     /locus_tag="PK016_04950"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HXL02449.1"
                     /translation="MKNWKEEIEKLKKEKNAIILAHNYQLPEVQDVADFVGDSLGLSM
                     EASKTDAQVIVFCGVFFMAETAKILSPQKVVLIPEREAGCPMADMITASQLLELKKRY
                     PEAATLCYVNTKAEVKAQCDFCCTSANATKVVEKGLSSASSIIFVPDQYLASYVSRKT
                     GREFIVWPGYCPIHAAISEKDIEEARALHPRAEMLVHPECRPEVTEKADQVLSTEGMC
                     RYVKSSPGDEFIIGTEVGILHRLQKENPAKHFYPVKEEMMCPDMKKINLEKIALALRE
                     MKYEVELPPSIIQKAALSIERMLQFT"
     gene            complement(6555..7877)
                     /locus_tag="PK016_04955"
     CDS             complement(6555..7877)
                     /locus_tag="PK016_04955"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXL02450.1"
                     /translation="MSKKILLVNPWIYDFTAFDLWLKPLGLLYLASHLLKRGYEVELL
                     DCLNRLDPDFPPTSRGPRYLREAGCGSYYKEILPQPSFFQDIPRYWGRFGLPFQKVKS
                     RMEKLSPPDFVFITAGMTYWYPGAVVAKELARKIWPGAKIVLGGIYPALCPEHAESWG
                     FDYVVKVTDPVSVMKKLGYILETDFKVENYSDYFSLKPAFFLYPELSYTVLLTSLGCP
                     FRCTYCASGKLYPYFWCRPVEDVIEEILFSREKYGIKDIAFYDDALLFQGEKHFLPLL
                     RNLEELSLDLRFHLPNSIHPRYVTPQIAQLMKKVGFKTLRMSLESSSSLWQEKTGGKV
                     DNYAFEKATRCFLDAGFGPQEIEVYLLFGLPGLSEKEMINSIKYVKSLGLQPRLSLYS
                     PIPHTPDYYKTERLLGDEPLFHNKIAFLYKSGNALLYQKLQRELSPKT"
     regulatory      8400..8574
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            8686..9624
                     /locus_tag="PK016_04960"
     CDS             8686..9624
                     /locus_tag="PK016_04960"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HXL02451.1"
                     /translation="MAKSNKTKGKIAFFLVGMWLLVFSLSFGVGAKTLSDDSGREVEV
                     PSSCERIISLSPSNTEIVFALSLAEKLVGVTTYCNYPEEAKEKEKVGTLNEINLEKVV
                     ELEPDLVLASSLTPPDVVERLGSLGITVFVLDPHNINEVMEDIEKVGIIAGVEARGKE
                     LRKAMEEDIREVREKVSHLSPDERPTVFHLIWHEPLWTAGGDTFINEFITIAGGKNAV
                     ADLSGYVTLDLEEFIRRNPDIITVVENHGDAENLPYQFLLSDGRLQVVKAIQEHKVFP
                     VDADIVSRSSARVVDAIHIFARILHPEIFGEYEYEK"
     gene            9608..10606
                     /locus_tag="PK016_04965"
     CDS             9608..10606
                     /locus_tag="PK016_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HXL02452.1"
                     /translation="MNTKNKKGFTYFGLVALVLGSVFLSQGVGGGRYHLSQSLSLFFP
                     SRLSPSEKVILFDIRLPRIILSFLVGGSLSLGGGALQGIFQNPLVDPYVLGISSGAAL
                     GAVIAILSGVYLGFFTIPLFAFSMALFSAFLVFYLGKIGKKFPLEVLLLAGIGVGFFF
                     SALVSLGMFLAGEDLHQLFFWTMGGLWNSSWREVKMILPFLIVGAPTLFSFSRELNAF
                     LLGEKVAESLGVEVEKVKKWLLFVVSLLVASSVAVSGVIGFVGLVVPHLVRLLGVRDH
                     RHLLPFSFLLGGGVLLWADTLARTILNPVELPVGIITNLLGAPFFIYLLRKRKKSL"
     gene            10603..11847
                     /locus_tag="PK016_04970"
     CDS             10603..11847
                     /locus_tag="PK016_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL02453.1"
                     /translation="MKEYLQVENLSFSYRERKVLDKLTFNVEKGELVGIVGPNGAGKS
                     TLLYLLAGLLTPEKGKIALGDKDTRFLSRRMWSQKVAMVFQEIPQNLELTSQEVIAMG
                     RFPHLGRWQREREIDEEKAAWAMEITSTTAFFDRLFSELSGGEKQRVMIARALAQEPE
                     LLLLDEPTSHLDILHQLEIMDILLELRKGGMAIISVFHDLNLVSQFCDKALLLKEGRT
                     LSFGGVEEVMKVEEVEKLFGVEFLETTHPFSLRPFFMPLQKIKNQSSWGIVHLICGGG
                     SGSPLMRELLEAGFSLSLGVVNQLDSDEELARKLGIPVVQEKPFSPFSPSTMEEAYRL
                     AQKADYIVIAPTYWGRGNLPNLDLAERLALEGKAVLVLEEALNPEFDYTEGEAREKLR
                     KIIKEGGKVVKDLPTYFSSLSP"
     gene            complement(12115..12693)
                     /locus_tag="PK016_04975"
     CDS             complement(12115..12693)
                     /locus_tag="PK016_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02454.1"
                     /translation="MRHWSKVAIVSLLLLLAIFPLEAREEEEEKAILEGRAFVPFITS
                     KGHVKERFFSWATVTVIDLRTGKVMAEGETEIDGSYEIYVPPQRSYLIQFQKDAQTIL
                     DVCRQVEKGKRYNLSFADARSTAMSLVLLHLFSSKKHPDIIIAYKANRILREESFPLL
                     QQKVLKAIARGKNPEDDLEIQLLIDRIALSVQ"
     gene            complement(12947..13432)
                     /locus_tag="PK016_04980"
     CDS             complement(12947..13432)
                     /locus_tag="PK016_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HXL02455.1"
                     /translation="MAQKTRELVKDIADELIRLLNKAYADELIAYFYYKTAALLVKGI
                     HAKTVAEQLEEIAKEELEHSEELADRIIQLGGEPLDDWDAITKNANYPKVDLPEDRAD
                     YAGILKAVHVAEQGAIGVYDDLMKFLQSHTKDPVTWHLIRHIMGEEMHHEEEVETLLG
                     L"
BASE COUNT         3860 a         2649 c         3133 g         3875 t
ORIGIN      
        1 tttatctctt ccagctgctt cttttctttc tcgtctattt gaccatctaa agaaaggata
       61 tattccattt gctccaccac caactccgat agaggtcgag tggggccagc taaaataatg
      121 acaccggcca ctttaccatc tcggcgagct atctctggaa ccagttgccc acccagactg
      181 tgtcccaaga taaacacttg ttgcgggttt atctcttcct ggtgacgtaa aaattcaaga
      241 gctgccaggg catcttctat aacctcttct tctacggtaa actgagatag gtcttcaatc
      301 ttatttccgt ggacgtaagt ccgtttatca tatcgtaaaa ctgctattcc ctgactggct
      361 aaaccccagg ccaggtcctt taatggttta ttagctaaca aagtttcgtc tctatcgttt
      421 gaaccggagc catgtatcag aactaaacca gggaatggtc cttctccttt aggtagtgtt
      481 aaagtccctg gtaactccca ccccggaatt ccaaaaacta cttctctttc tataatttta
      541 tcttcttcta catagtctgg cagggaatac tcaatttgag aagaagggag aagttgaaaa
      601 ccaatgattt tcccctcctc gctgaatact aatttcgctt ttaattctgc tttttcaaac
      661 tcgcaccggg cataaactac ctgatatcct ccttccacgg ctacttgatg gtctttcatc
      721 tctttgagtg gacctaaatc gctggttaaa ttctcccagg tttcctttaa agcttcttca
      781 ggaagtgctt cttgcacctc gggaataaat agctcttcag ccaagtggta ttttcccgtc
      841 ctcaaatact ggaaaaaact tttcgatagc tccaagagtt cttccgagga aagccggact
      901 ttttcttctc ccataactgg agtaattacc agaaaagaaa gagccagaat aattgttatc
      961 cataacaatt tgttcattat tgtgcctcct ttatcatatt ctaacattat ctctcaatta
     1021 tgacgagaga aagaaaagga tggtgaaaga aaggacggtc gtaactttat ttaaactcgg
     1081 cttacccccg gggcttgaaa aaattgttct tcatgctatt atattatcag taagagcgga
     1141 aatagctcag gtggtagagc atcggcttcc caagccgagg gtcgcgggtt caaatcccgt
     1201 tttccgctcc atttttatag gcctctcaga catttttaca gttccctttc cacttctaga
     1261 tcgaacctgg tttaactgct cttaagaaag gattattttt agatcgccct ttatagtatc
     1321 tacaaaattt gttataattt ctctttgagt gagaaagaaa ggcaactgcg ttactctttt
     1381 gcttcataat ttctaaaaaa gcgatgaata taataggagg gaatgtttta tgaagaggca
     1441 aaagcatgtg ttgatgctgc ttcatctagg tctttttctg ctgattttta gtgtattgat
     1501 tcctgcaact gctgtgtttt cttcaacttc gctgggccag agctatcttt tgttaacggt
     1561 ggacttagaa aaagaactgg ttgtgattaa aaatgtttct gataagacga ttgacctaac
     1621 tggttatgta ttggtaagtg ttaaagggaa ccagcagttt acattcccgg caattgcgct
     1681 taagccggga gaaacagtga ctgttacatc tggcaaaaat gcacaatctg acccgccacg
     1741 ctatttactc tggactaaac aaaatatctg gaacaataac ggagaccctg ccaaactttt
     1801 ggctccagac gggaaacttg tagttgctta ttgaagtgga tgtctctgtg aagaaacacc
     1861 cccggataag tttttactct gtcaattcct ttaccgggtt tactattttg ttggtgtgga
     1921 tttcgtctcg ccagtaattt taagaaatct ctccggccaa ttctgcatac cgattttaaa
     1981 tcatcttccg taattcctga atgtttcgaa atataaagag gctttgctcc ataactctga
     2041 aaaaaacaac aaacataaaa agccactgag ggagagggta tttttcagta aaagcgtgga
     2101 tttccgattg aagatttcgg ggatgacgaa tgaggaggat ttctcactac agtttgacag
     2161 gcagtatttt cacttctata atattattaa ggtaatcagg attataaaat ttgggagtga
     2221 taaaaatccg tttttctact cgttcccgtt atggattaag atttatgata gagctggcta
     2281 cttattacgg taaaaaagac ctggtctttc tcaaagaaat agcagagaaa gaaggtattt
     2341 cagaaaaata tttgactcaa ttagctattc ctcttaaggc tcggggttta atttcttccc
     2401 ggcgaggagc aggaggggga tatcgccttt ctcgccatcc cgctcaaatc acggcaaaag
     2461 aggtgataga aactttagag ggagggattt tccctgtaga ttgtctggaa agtcccgata
     2521 tctgttcccg agcctctttc tgtgcagcaa gaaaaatgtg gaaagagctt caagacaaaa
     2581 tttccgagac tttagaaaag tttactctgg aaaacttagc tcagatgtct accaataact
     2641 ctcactctcc aatagactat tccatttagg agaactgatg aaagcaattg gtcttatatc
     2701 tggaggtctg gatagtgcat tagctgtttt tttgcttaaa aaacagggga tagaggtgat
     2761 tggtctcaat ttccatagcc ctttctggga aagcaaaaac acattgaggc agttagctca
     2821 agatttagag atagcgctcc gggcagtaga agtaagggaa gattatcttg agctcgtgag
     2881 aaaccctcaa tttggatatg gaaaaaactt gaacccctgc attgattgta aaatctggat
     2941 gcttagagag gccaaaaaag aaatggaaaa agaaggagcc tcttttattt ttaccggtga
     3001 agttgtggga caacgcccca aatcccagct aaaaaatact ttacgtttga ttgaaaggca
     3061 aagtggactg gaaggttatc ttttacgccc tttatcggcg aagcttcttc ctcctaccat
     3121 tcctgaagaa aagggttggg taaaaagaga ggagctgctt gatttacaag gaagaggaag
     3181 aaaaaggcaa ttggaattgg cggaaaagtg ggggataaag catttttccc ctccggcagg
     3241 ggggtgtctg ttaacggatc ccaatttttc ccggaggtta aaagacttgt gggagagcga
     3301 agaggcttat gattttgaga gtgtggaact actaaaggta ggaaggcatt ttcgcctcgg
     3361 cgcttctttt aagcttgtag tgggaagaaa tcaagcagag aatgaaaaac tgaaagaatt
     3421 tctccgggaa ggtgatatat tgtttttccc ccgggagggg aagggccctc ttgctctggg
     3481 tcgggggaaa gttgatgagt ctcttcttca agaggcagct tccctttttg cctactatat
     3541 tcgctcccat ggcgaggcgg tttctgtagg agtgaagttt gactcatttg gggaagtgat
     3601 agagatagag agaatgagtg aagaagaagt aaaaaagcga atgatttaag aggtgagtta
     3661 tgaggataaa agatgacatt acccagttgg tgggaaacac ccctttagtg aagctgggga
     3721 aaatcgggaa aaacttacca ggagaactga tagcaaaatt agagtttttt aacccctgtg
     3781 ggagtgtcaa agaccgaatt gccgtagcaa tgatagaaga agcagaaaaa caaggctttc
     3841 ttaaagccgg gtctttagtg gtagaaccta cgagtggcaa taccggtatt ggtttagctt
     3901 ttgtatgtgc tgaaagagga tatcatcttc ttttgactat gcctgaaagt gtctcggtag
     3961 agaggaggaa aattttaact cgcctgggag ctgaagtggt tcttactccc tcatcagaag
     4021 gtatgcgagg agcaatagaa gaagcgaaaa aaattgcggg aaataccccc agagctttca
     4081 tgcctcagca gtttgaaaac ccggctaatc ctcgcgccca ccgagaaact actgcccgag
     4141 aaatctggga ggataccgat ggtaaagtag acattgtggt tgcgggagtg ggaagtggcg
     4201 gaactatcac cgggattgct caagctctta aagagaaaaa accagctttt caggcggtgg
     4261 cagtggagcc tcttcgctct ccagttttat caggaggctc tccggggcag cactctattc
     4321 agggaattgg agcagggttc attcctccgg ttttaaagct ggagctcctg gacgaggtaa
     4381 ttgctgttag cgacgaggac gcttttttga tgtctgagcg cctggcaaaa gaggagggga
     4441 tactggcagg tatctcttct ggtgcggcac tgtggggggc ggtacaggtg gccactcgag
     4501 aggaaaataa ggagaaactc attgtagtaa tcattcctga taccggggag cgttatttat
     4561 ctctttggtg agcgaagtga gaaaaaaagt actggtagct atgagcgggg gagtagattc
     4621 ttcgctatct tgcgttttgc tcttagaaag aggatttgaa gtcgcaggag taactatgtg
     4681 tctgggaaaa aaaggtaagg aatctgcctg cctggttgat gtggctgtgg aagatgcccg
     4741 gcaagtttgc cgcactcttt ctattcccca ttatgtggtg gatttttccc gggagtttga
     4801 agagttagtc gtggaagatt ttgtttctca atatttgaaa ggcaagactc ctaacccttg
     4861 cgtcgtatgc aatcagcgtc ttaagtttgg taaactcctg gggtatgcgc gtgatcaagg
     4921 gtttgatttt ttagctactg gccattatgc taaaatcgaa gaaaaagagg gaaaatactt
     4981 tttaaaaagg cctaaagacc gaaggaaaga tcagacttat tttctctatg gcattggaag
     5041 aagcaatctt ccttacatca tttttcctct cgctgatttt actaaagaag aggttagaaa
     5101 gttatctttg aaatataatc ttagtatagc ggagaagtca gatagtgaag atatttgctt
     5161 tctccccgaa ggtgactacc ggaaattcct ggagggacgg gtctctctgg attctcccgg
     5221 ttttatagtt accacagaag gtaaaatagt gggagaacac aaaggatatt ttcgctatac
     5281 cattggtcag agaaaaggct tgggtgtggc ccatcctcgg cctctttatg tagtggcggt
     5341 acatccggag agaaatgaag tggtggtggg agaaagagag gtgctccagg ctcagggatt
     5401 aatagcttct catttccatt ggctggttga tgaactcccc tcatctgtgt tcgctcaggt
     5461 gcgctatctc cagagggaaa agccttgctc ggtaagtatt attaattcag aaaaggttca
     5521 ggttatattt aaagaaaaag tggaaatgat cagtcctggc caatcggtag ttttgtatag
     5581 tgatgataca gttttgggtg gagggattat tgaagaagct ttttaagatt tccgataggg
     5641 agtgaaaaga tgaaaaactg gaaagaagaa atcgaaaaac tcaaaaagga gaaaaatgca
     5701 ataattttag cccataatta tcagcttcct gaagtacagg atgttgctga ctttgtagga
     5761 gattcactgg ggttgagcat ggaggcaagt aaaactgatg ctcaggttat tgtgttctgt
     5821 ggggtttttt tcatggctga aacggcaaaa atcctctctc ctcaaaaagt ggtgttaatc
     5881 cccgagcgag aggcaggatg tcccatggca gatatgatta ctgcttccca gcttcttgaa
     5941 ctaaaaaagc gctatcctga ggcagctaca ctttgctatg tgaatactaa agcagaggta
     6001 aaagcacaat gtgatttttg ctgcacttcg gctaatgcca ctaaagtggt agaaaaggga
     6061 ttgagctctg cttcctccat catttttgtt cctgaccagt atctggccag ttatgtttct
     6121 cgaaagacag gaagggaatt tatcgtctgg ccaggatatt gtcccataca tgcggcaata
     6181 agtgaaaaag atatagagga agctagagcg cttcatcctc gagcagagat gcttgtccat
     6241 cctgagtgcc gtccggaagt gacagagaag gcggatcagg tgctctctac tgaaggtatg
     6301 tgtcgttatg tgaagagctc ccccggggat gagtttatca ttggaacaga agtgggtatt
     6361 ctccatcgct tgcaaaagga aaatccggcg aaacattttt atccagtgaa ggaagagatg
     6421 atgtgcccgg atatgaaaaa aataaacctg gaaaaaattg ctctggctct ccgggaaatg
     6481 aaatatgagg tggaacttcc cccatcgatt attcaaaaag cagcgctgag cattgaaaga
     6541 atgctccagt ttacctaagt tttcgggctc agttctctct gtaatttttg atagagaagg
     6601 gcattgccgc tcttgtaaag gaaagcgatt ttattgtgaa agagaggctc atcgccgagt
     6661 agtctttcgg ttttataata atcgggagta tgggggatag gagaataaag agacaggcga
     6721 ggttgtaatc ctaagctttt tacgtattta atggaattta tcatctcttt ttcagagagt
     6781 cccggcaagc caaagaggag atatacttct atctcttgag gaccgaagcc agcatctaaa
     6841 aagcatcttg tggctttctc aaaagcgtaa ttatctacct ttcctcctgt tttttcctgc
     6901 cacagagagg aagagctttc taaactcatc cgcaaagttt tgaaccccac tttcttcatc
     6961 aattgagcga tttgaggagt aacataccgg gggtggatag aattgggcag gtggaagcga
     7021 aggtctaaag ataactcttc taagtttctt aagaggggaa gaaaatgttt ctctccctga
     7081 aaaagaagag catcgtcata gaaagctata tcttttattc catatttttc tctggaaaat
     7141 aaaatttctt ctattacatc ttctacggga cggcaccaga agtaaggata gagttttcct
     7201 gaagcacaat aagtgcagcg aaaggggcaa cctaaagaag tgaggaggac ggtataagat
     7261 agctccgggt aaagaaagaa agcaggtttc aaggaaaagt aatcactata attttccact
     7321 ttgaagtctg tttctaagat atatcccagc ttcttcatta ctgatacggg gtcggtaact
     7381 tttaccacat aatcaaaacc ccagctttca gcatgctccg ggcaaagagc aggataaatt
     7441 cctcctaaaa ctattttagc ccccggccag attttcctgg ctaactcttt agctactact
     7501 gctcctggat accagtaggt catacctgca gtgataaaga caaaatcagg aggggataac
     7561 ttctccattc tggattttac cttttgaaaa ggtaaaccaa accttcccca gtagcggggg
     7621 atatcctgga aaaaagaagg ttggggaaga atttctttat aatagctacc acagcctgct
     7681 tctcgcaaat agcgaggacc acgagaggta ggtggaaaat cggggtctaa acggttcaga
     7741 caatctaata actctacctc atacccccgc tttagaagat gagaagcaag ataaagaagc
     7801 cctaaaggtt tgagccagag gtcaaaagca gtaaagtcat aaatccaggg gttaactaaa
     7861 agtatttttt tgctcataat aggattataa cagaactatg agggggaaaa gaccccctca
     7921 taaccccgaa ctataaaggg gcgatgcccc tttataatcc ccaaaaaaca aaaaaataca
     7981 aaaagaaaaa gactactgag gaaggaaaag catcttcctc agtggggtaa acccctttat
     8041 aatcccgaac tataaggggg taaaccccct tataaccccc caaaagcaaa aaacaaaagg
     8101 aagaagcaaa aagttaaaaa ctgaaaaaat ataaaagact aactgaggaa agaaaaacgc
     8161 tttcctcagt ataagggaga aagaattccc ccttatgaat cccctttctt tttcctcgcc
     8221 ttcaatggaa gaaccccaca tattccttcc ctcgccctcg aagggagagg gttagggtga
     8281 gggtgaaagc tataagaaag cttgtccctt tatagtcccc caaaagcaga cagcctacgg
     8341 cgtaagacta aaaaactact gagggagaaa aagcgtcttc ctcaggagtg tgatataatc
     8401 tctgtggaat ggtttcaggt ggttttacct taatagggaa gtccggtgaa aagccggcgc
     8461 ggtcccgcca ctgtaaccgg ggagcgagtc gccagagcca ctgacgaaag tcgggaaggg
     8521 gtaacgagca aagatccggg agccaggaga cctgcctgaa attatcccgg ccactcttcg
     8581 tggaaagagg aggtgggaaa tttcccggat tccgccccgg ataagaggat actccctctc
     8641 ttccctctcc tcctccacta aaataatata agaggaggaa atggcatggc aaaaagtaac
     8701 aaaacgaaag gtaaaatagc atttttcttg gtaggaatgt ggttgttagt tttttccttg
     8761 tcttttgggg tgggagcaaa aactctctct gacgactcag gaagagaagt ggaagttccc
     8821 tcatcgtgtg agcgaattat ttccctttct cccagtaata cggaaatagt ttttgccctc
     8881 tctcttgcag aaaagcttgt aggagttact acttattgta attatcctga agaagcaaaa
     8941 gagaaagaaa aagtaggcac tcttaatgaa attaatttag aaaaagtagt agaattagag
     9001 cctgatttgg ttttagccag ctctttaacc cctcccgatg ttgtggagcg gctgggaagt
     9061 ttagggatta ccgtttttgt cttagacccc cacaacatca atgaggtaat ggaagatata
     9121 gaaaaagtgg gaataatagc aggagtagaa gccagaggaa aagagttaag gaaagctatg
     9181 gaagaagata ttcgcgaggt gagagaaaaa gtttctcatc tttctccaga tgagcgacct
     9241 acggtttttc acctcatctg gcatgaacct ctctggactg ccggaggaga cacttttatt
     9301 aacgagttta ttaccatcgc tggtgggaaa aatgcagttg ctgacctgtc tggttatgtt
     9361 actctggacc tcgaagaatt tattcgccgc aaccctgata tcatcactgt ggtagaaaac
     9421 cacggagatg cagaaaactt accttaccag tttttgctct ccgatggccg attacaagtg
     9481 gttaaggcaa ttcaggagca taaagttttc cctgtcgatg ccgatattgt ttctcgctct
     9541 tccgcccggg tagtggacgc cattcacata tttgcccgta tacttcatcc tgaaatcttt
     9601 ggagaatatg aatacgaaaa ataaaaaggg gttcacatat tttgggcttg tagctttagt
     9661 tctggggagt gtttttttat ctcaaggagt agggggaggg agataccacc tttctcaatc
     9721 tctttccctc tttttccctt ctcgtctttc tccatcagag aaggtaatat tatttgatat
     9781 tcgtctgcct cgtattattc tttctttcct cgtgggagga agcctctctc tcgggggagg
     9841 ggcactgcaa ggaatttttc agaatcccct ggtagacccc tatgtcctgg gtatcagctc
     9901 tggtgctgca ttaggagcgg taatcgccat tttgagcggt gtgtatctcg gatttttcac
     9961 catccccctc tttgcttttt ctatggctct tttcagcgcc tttctggttt tttacctggg
    10021 gaaaataggg aagaaattcc ccctcgaggt tttacttttg gctggcatag gggtgggatt
    10081 tttcttttct gctctcgtat cgctgggaat gtttttagct ggagaagacc tgcatcagtt
    10141 gtttttctgg accatgggag gtctgtggaa tagtagctgg cgagaggtca aaatgatttt
    10201 acctttcctt attgtgggag cccccacttt attctccttt tcccgggagc tcaatgcttt
    10261 tctcctggga gaaaaagtag ctgaaagttt aggggtggaa gtagaaaaag ttaaaaagtg
    10321 gctgctcttt gtagtatcgc tcctcgtagc atctagcgtt gcggtgagcg gagtaattgg
    10381 atttgtagga ttggtagtac ctcatctggt tcgccttctg ggcgtgagag accatcgtca
    10441 tctcctgcct ttttcttttc tcctgggagg aggagtactt ctctgggctg atactttagc
    10501 tcgtaccatt ttaaacccgg tagaactacc ggtgggaatt ataactaacc ttctgggtgc
    10561 tcctttcttt atctatcttt tgcgaaagag gaagaaaagc ctgtgaaaga atatttgcaa
    10621 gtagaaaacc tttctttttc ttatagagag agaaaagttt tagataaact taccttcaat
    10681 gtagaaaagg gagaattagt agggatagta ggcccaaatg gagcaggcaa aagcactctc
    10741 ctctatcttt tagctggctt acttaccccg gagaaaggga aaattgccct gggagataaa
    10801 gacaccaggt ttctttcccg ccgaatgtgg tcacagaagg tagctatggt ttttcaggaa
    10861 attccccaga acttagaact cacctctcaa gaagtaatag caatgggaag attccctcac
    10921 ctcgggagat ggcagagaga aagagaaatt gatgaagaaa aagctgcctg ggctatggag
    10981 ataacctcca ccacggcttt ttttgaccga ctgttttcgg agctctcagg aggagaaaaa
    11041 caaagagtaa tgattgcccg tgctttagct caagagccag agctactgct gctggatgaa
    11101 cctaccagcc acttggatat cttgcatcag ctggaaatta tggatattct tcttgaactt
    11161 cgcaaaggag gaatggccat tatttctgtt tttcatgacc tgaatctcgt ttctcaattt
    11221 tgcgataaag cacttctttt aaaagaagga agaacgctct ctttcggagg ggtagaggaa
    11281 gtgatgaagg tagaggaagt ggaaaagttg tttggtgtgg aatttctgga aaccactcat
    11341 cctttttctc tccgcccctt ttttatgcct ctgcaaaaga taaagaacca atcttcctgg
    11401 gggatagtac acctcatttg tggagggggg agtggctcac ctctgatgag ggagctatta
    11461 gaagctggat tttctctctc tttaggagtg gttaaccaac tggattctga tgaagagctg
    11521 gccagaaaac tgggaatccc ggtagtgcaa gaaaaacctt tttctccttt ttctccttct
    11581 actatggaag aagcttaccg attggctcaa aaagctgatt atatagtcat tgctcctact
    11641 tactggggaa gaggtaatct ccccaattta gacctcgccg aacgcctcgc tctagagggt
    11701 aaagcagtgt tagttttaga agaggcactg aatcctgagt ttgattacac cgaaggagag
    11761 gcacgggaaa agttgagaaa aataataaaa gagggaggga aagtggtaaa agacctcccc
    11821 acctattttt cttctctttc cccttaggaa gttttactga ggggtaagaa gcttgctcca
    11881 gtaacctcct tgtgtttttg tggctacttc ctcagtattt ttgtcttttt atcttacgtc
    11941 gtagactgtt tgttctccgc aatgacaaaa agacgttagt aacaagagga caacaccgca
    12001 aaaaaacagg cttaagtgac attgatatag aaaacttgat tttaacctta gagaccacga
    12061 aagaaaccat gggagggaac atcttctgta aaaatactgc aattatactt atttttactg
    12121 gacacttaag gcgatgcggt caataagaag ttgtatctct agatcgtctt ctggattctt
    12181 tccccgggca atagctttaa ggactttctg ctgaagaaga gggaaagatt cttctcgaag
    12241 tatacggttg gctttatagg caatgataat gtctggatgt tttttgctag aaaaaaggtg
    12301 taaaagaacg agactcatgg ctgtggagcg ggcatcagca aaagagaggt tatatctttt
    12361 tcccttttcc acttgcctgc aaacatcgag gattgtttgg gcgtcttttt gaaattgaat
    12421 aagatacgac ctttggggag gaacatatat ctcataagag ccatctatct ccgtttcacc
    12481 ttccgccatt acttttccgg tgcgaaggtc gataacggtc actgttgccc aggaaaagaa
    12541 tcgttcttta acatgccctt ttgaagtgat aaaaggaaca aaagctctgc cttcaagaat
    12601 agctttttcc tcttcctctt ctctggcttc caggggaaaa atagctaaaa ggagaagaag
    12661 acttactatt gctactttag accagtggcg cacaggttta atacctcctt cttaaaacat
    12721 ctgaacagga aaaaacccga ccaaaaataa attgtagctt acaaacgaaa tattatcact
    12781 gactcgaaaa ctcaataata tccgacgata atctgatagt acatggaaga aagatggtat
    12841 cttcctggta tatgtatacc ttttaggctt ggtaactttt ggagattata tcggaaggat
    12901 tatctaaaaa aagaaggggt taaaaaaccc ttcttttttt agatttttac aatcctaata
    12961 aggtttcaac ttcttcttcg tggtgcattt cttctcccat tatgtgtcgg atgagatgcc
    13021 aggtgacggg gtctttggtg tgggactgca agaatttcat aaggtcatca taaactccta
    13081 tggctccttg ctctgctaca tgtactgcct ttagtattcc tgcataatca gctctatctt
    13141 caggcaggtc tactttaggg tagttagcgt ttttggtgat agcgtcccaa tcatctaatg
    13201 gttctcctcc cagctggatg atccggtcag ctaattcttc tgagtgttcg agttcttctt
    13261 tggcgatttc ctctaactgc tcggcaaccg ttttggcatg gataccctta accaacaagg
    13321 ctgcagtttt gtaataaaag taggctatta gctcgtctgc ataggctttg ttaagtaatc
    13381 taattaactc atcggcaatg tctttaacta attcccttgt tttttgcgcc attttcgatc
    13441 cctccctcat aaatgtttta ttggtgaagc tgctgcaagc tcattttaac ttaatccggc
    13501 ttttgatgtc aacagca
//
LOCUS       DAOSVE010000043        13457 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_2743_length_13457_cov_69.697209, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000043 DAOSVE010000000
VERSION     DAOSVE010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 13457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13457
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2743_length_13457_cov_69.697209"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PK016_04985"
     CDS             <1..486
                     /locus_tag="PK016_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXL02456.1"
                     /translation="GKVVDELTGEGLYGAEVTVRDYPTRSDLTASDGSFFLPLVPAGR
                     QVIIATLYGYATKSEAVNIPREGIFELTVELSPLLGKLMGYVFSEDGKPISGAEITLN
                     GDYHTTSGADGSFVLSNLPVGKFVITVEKVGFLPYSDEVEIVANSITTLEITLNHLTS
                     K"
     gene            534..2492
                     /locus_tag="PK016_04990"
     CDS             534..2492
                     /locus_tag="PK016_04990"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HXL02457.1"
                     /translation="MGDTFDVSHYLGVFLEEAKEHIESLEENLVELEKESDNQDLIQA
                     IFRSFHTIKGSSGSMGFQQMAELAHLLESLLDKVRRGEVSVSPSLIDLLLQGVDFLKR
                     MLEEVAEKRQEPDWDMKEIFSALQNFREEREEESNEEYWLTVKLASDCSMKGVRAYLV
                     ISRLQDSGIGVVSTVPSLSDLEGENFGQSFDVLVHTTLSPGEVAKIVESIAEIESVEV
                     KGEEENNQIEEKEEKEEKNAIFSPGEQRITANLKKTVRVDVERLDSLLNLVGELVIDR
                     TRLEDVIQRLKEYQELVQFREILSDATTHIGRITNDLQMEIMKARMLPLAEVFNRFPR
                     LIRDLARQSGKEINFIIEGEETELDRSLLEEITDPLIHLLRNAVDHGIESREERINKG
                     KPSQGKVVLRAYQEENSVVIVVEDDGRGLDIEKIRDKAVKMGLVTPEEAGKMTEREIV
                     DFIFYPGFSTSDKVTEVSGRGVGMDVVKRNIERINGQVRVESQLDAGTTFYLRLPLTL
                     AIIRALLVRVGESIYSIPLSDVVEIEKVEKKNTYWVNRVLTTLFRGKTLPLLWLGALL
                     DFQRYDFITEDEIPEILYTVVVNASHSLAGLVVEEVLGEQEIVIKSLGSYIGDVRGLG
                     GATILGDGGISLIIDVASLLWKFSQAGG"
     gene            2499..2864
                     /locus_tag="PK016_04995"
     CDS             2499..2864
                     /locus_tag="PK016_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXL02458.1"
                     /translation="MPGRIMIVDDAAFMRMMLKDILTKNGYEVVAEASNGQEALLKYE
                     EAKPDLVTMDITMPEMDGITAVKEIRKKDPQAKIIMCSAMGQQAMVVDAIQAGARDFI
                     VKPFKPDRVIEAVKKVLGN"
     gene            2881..3984
                     /locus_tag="PK016_05000"
     CDS             2881..3984
                     /locus_tag="PK016_05000"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXL02459.1"
                     /translation="MKKISSLSSFLCQIPLGVIIFTRRGRVMYWNKEMEKITGRDKKE
                     AIKKEVKEILGFDFSWEEIQRSLEFEEEFFIRNWPIKDHFEEEHLLNLHFFLWKKEGK
                     KTNVMCLVEDVTQKARWGENIRQMEKLSSISRFIASMAHEINNPLTVISGYTQMLLQK
                     AKENRQCEVCRATQRGLEKIEKEAEYCGRLIQELVDFSKPMILNKEDIDLNFLVQDSL
                     SVLDVLMDDKIKLELNFSNDVLLVRVDKSRMRQVFMNLAKNALEAMKEEGGKLQINTR
                     IAPLEHFSFPENGTGNGRFVEIEFIDQGKGIPEKTLPHIFEPFFTTKEKGTGLGLSIS
                     YSIVRAHKGWIEVSSQEGGGTRIKVFLPQGGGN"
     gene            3986..5311
                     /locus_tag="PK016_05005"
     CDS             3986..5311
                     /locus_tag="PK016_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02460.1"
                     /translation="MELRADFRLVQRPQLIPKMAQAVEMMALPLPQIEQKLQKLIEEN
                     PLLEVREGSVCYNCGKSFSGYPERCPYCGRYLRRREKEEFSGEEFPQWKEKKDWKEMM
                     KEEIFSFIELCPKEKRVLHFLIARLDGEGFLREDLEKLSFLLGEEDNLEKIIEKVRKE
                     GFLGFAARDAIDFLILQMIELGLIKKEEKDKVRRELLSHPHKYQKLLEPYQEKLFFSP
                     VQFFETSLGREAGELESSLSFLIPDAEILEVGENSFEVLLTPSLYFQLVINEEVLSFY
                     RKRRKETPEREKIFWQEKLGEAREVISCLNYRNQLLLRVLDYIVEREKDFLLHGFRYF
                     VPLTQREIAEGVGVSISAVCQVLKEKSVCFPDGVTRSVKFFFDNSYPVKEMMKIILTR
                     ETSHHPLSDQEIMKELEKKGFRIARRTCALYREEMGVLPSYLRKRLKEK"
     gene            5406..5801
                     /gene="flgB"
                     /locus_tag="PK016_05010"
     CDS             5406..5801
                     /gene="flgB"
                     /locus_tag="PK016_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HXL02461.1"
                     /translation="MRNEIIQDPVFRLLERGLDLSLARQSLLINNVANTETPGYQRQD
                     LNFEACLKETEKKGDLQLTHPNHLAPGKGEGTITEGVEQYSVRQDGGGVDMEKEMTLI
                     LENALYYQTLTRVVSKKLGLLGTVIREVR"
     gene            5803..6198
                     /gene="flgC"
                     /locus_tag="PK016_05015"
     CDS             5803..6198
                     /gene="flgC"
                     /locus_tag="PK016_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HXL02462.1"
                     /translation="MKIFRNFDVSASALTAERLRMDVISSNVANVDTTRTQDGGPYRR
                     KMVVFEVAGDGGVRVNKIQEDFSTPLQLVYQPGHPDANADGWVEYPNVVLVNEMVDMM
                     AATRAYEANVAALNATKSMMSSALNIGRE"
     gene            6210..6503
                     /gene="fliE"
                     /locus_tag="PK016_05020"
     CDS             6210..6503
                     /gene="fliE"
                     /locus_tag="PK016_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HXL02463.1"
                     /translation="MRIESNVPSLFLGLEGEQPSGTSREIGGFGECLNRALQKVNNLQ
                     IEADQMAEKVARGEEDIHNALIALEKANLALGLTVQVRNKVVEAYQEIMHMQV"
     gene            6511..8100
                     /gene="fliF"
                     /locus_tag="PK016_05025"
     CDS             6511..8100
                     /gene="fliF"
                     /locus_tag="PK016_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HXL02464.1"
                     /translation="MKNPLTEFAERAKKTWEGLNQGQRLLFLLISTLLVAGIVTLFLY
                     AGKPDYAPLFQNLSVEEAGKVMDKLKELGIPYQLSSGGTRILVPQDKVYEARMKLAQE
                     GLPSQGEGFEIFDKNTFGLTSFLQRVNYQRALQGELERTIMELKEVEGARVHLVVPEE
                     RLFAEEEKPPTASVVLKLRSGVTLQDAQIEAIANLIANSVEGLKPDRVAIIDDRGNIL
                     ASGSGDESSWTSLSRLTATQLEVKKEVEATLTNRIQSMLESALGYNQAVVRVTADLDF
                     EQREGQKETFEPIRGGEGIARSAQETEEKYQGGVTAPGGVPGVASNIPVYGQESEATG
                     QTNDYTRRESTVNYEINRVMERFASSPGKVTRLSVAVLVDNNLPSEQVEKIEGVVKAA
                     AGLNEERGDMLIVESLPFNREAQEEEKKALASQNMMKWIELLAKYGLIGILIFVMYSL
                     GRRILVSVAVPEKVTAEFYEEPEMEYEEEKLPPIKPVELSPEEKVKMEVQKRMEEDIK
                     KLVHTSPEDAVKLLRSWLHED"
     gene            8104..9126
                     /gene="fliG"
                     /locus_tag="PK016_05030"
     CDS             8104..9126
                     /gene="fliG"
                     /locus_tag="PK016_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HXL02465.1"
                     /translation="MAIVQRKSLTGRQKAAIFLVSLGPDLAAQVLKRLTDEEIEDITL
                     EIATLEKVPPELREEVIGEFYHLALAQQYISQGGIDYARQLLEKAVGPHKAQEIISRL
                     SATLEITPFESLRRADPLQLVNFIQDEHPQTIALILSYLKPEQAAMVLGNLPESVRGE
                     VAMRVALMDRTSPEVVKEIEAVLERKISNFLTTQDFTRVGGKKKLVEILNKSDRGTEK
                     SIIEALEERDPELAEEIKSEMFVFEDIITLDDRAIQLVLRQVDMKDLALALKGASPEL
                     QDKIFHNVSQRAAQMIREDMEYMGPVRARVVNEAQQKIVNVVRHLEEAGEIVIARGGG
                     EDEIFV"
     gene            9119..9838
                     /locus_tag="PK016_05035"
     CDS             9119..9838
                     /locus_tag="PK016_05035"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HXL02466.1"
                     /translation="MFRLYKEVKFLPDLFLVGEEDNNDGKVIVKQEEKEDNEDKFQEE
                     KSLIIEEAKRKAQMILEEAESERKNIEDKACREGREEGRRIGKEEVQKQWQEKVVVWE
                     RELREIYNYREEMIEKMKEPLLELSFIIARKIIEREAEREPFIERLVEKALKKLSNRE
                     RVVLRVSEEEYNLVREIREELMRKIDGIDYLEIQEDRRLKRGECLVETSFGSIDARID
                     TQLENIKEELFQALEEGTTSA"
     gene            9831..11147
                     /gene="fliI"
                     /locus_tag="PK016_05040"
     CDS             9831..11147
                     /gene="fliI"
                     /locus_tag="PK016_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HXL02467.1"
                     /translation="MLDFSRVKRIVEDIDPLRLNGKVLQSVGIVMESQGPSCRVGELC
                     RVRSLDRENEILAEVVGFRGERTLLMPLGERNGIELGSEVIALGKEMKIKVGEDLLGR
                     VLDALGRPMDGKGPVEGETEYPLDCEPPPPLDRKPIREVLPLGIKAIDGVLTCGKGQR
                     VGIFAGSGVGKSTLLGMIARYAQSDVNVIALVGERGREVKEFIEKDLGEEGLRRSCLV
                     VATSDKAPLLRIKAAFCATAIAEYFRDKGKDVLFLMDSVTRLAMAQRELGLAVGEPPT
                     TRGYTPSVFTLLPRLMERTGNSRRGSITALYTVLVEGDDMNEPIADAVRAILDGHIVL
                     SRKLSFEGHYPSIDVLQSISRLMPDITDEKQQQMAQKLREILATYSEAEDLINIGAYE
                     QGSNPRIDYALTMIDRVKKFLRQEVGESFSWEETQAFLSQLFEGEK"
     gene            11148..11600
                     /gene="fliJ"
                     /locus_tag="PK016_05045"
     CDS             11148..11600
                     /gene="fliJ"
                     /locus_tag="PK016_05045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HXL02468.1"
                     /translation="MKGYQFKLQKVLDVRKVKEDLIGARVAELEEIIEAQKGHLLSLE
                     KKKEGVLVERREKREIDNGFRIEGEMFYEWYLENLERKVEVVKTSLKQLEEQMRKTRE
                     ELIKAAVDRKIMEKLKDKDYENFKVEVIRNDQKNLDEITIQRYKRGIE"
     gene            11602..12090
                     /locus_tag="PK016_05050"
     CDS             11602..12090
                     /locus_tag="PK016_05050"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HXL02469.1"
                     /translation="MLPYNLEKIISRIGEIESRFSQRVSSLEERTIRGDDNYAASQDT
                     FDDLIKEYAQKYQIDHRLAKRLIEAESNFDPDAVSPRGAMGLMQLMPQTCQDLGIKDP
                     FDERENLEGGMRYLRGLLNQFGSLPLALAAYNAGPSRVKEYGDIPPFPETQEFVKKVI
                     GG"
     gene            12099..12707
                     /locus_tag="PK016_05055"
     CDS             12099..12707
                     /locus_tag="PK016_05055"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02470.1"
                     /translation="MASREMDIQKRKEKTANNKTSKKKKSNWIETILPVLVFAFTLVF
                     FLNFSGVIDLPFLHFMRLPSVEKEDSENLMVKEVEKETESQVVYPGPTLERVEPEESP
                     VIEEPLEEEVEVLPEIEEPSSGKTDGEDVDSSAREERIFRLANIYTAMEPEEASRIME
                     KLSDEEVVEILSVMKERNVAEILNAFPVERAAEIMKKMMEGR"
     gene            12713..>13457
                     /locus_tag="PK016_05060"
     CDS             12713..>13457
                     /locus_tag="PK016_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02471.1"
                     /translation="MDGFIFLPVESGEMKGNSFTVDKIEKEEGKESVGFEQLLSPFLF
                     SWGTGEVTPVKSCSSSSSVSNCSLERSTCSPCSLSGADTFSSGACSLSSSSSLEEMLN
                     VEGWQKTIDSFLSEVENISFTLNLGEKGKVSVSGGTGEDGKFTFQISGDKEALSELLG
                     RVVKQVVAFLSEQGFSYPGGESSFSECSLGQGCGGGCGGEEIITSQIGEIQGDTELGY
                     IIPDDLLPPEEGEENAIFSDVSLLPDVEVE"
BASE COUNT         4294 a         2050 c         3469 g         3644 t
ORIGIN      
        1 ggaaaagtag tggatgagtt aactggtgag ggtttgtacg gtgcggaagt gacagtgaga
       61 gactatccta cccgctctga cctcaccgca tccgatggtt ctttcttttt gcctcttgtt
      121 cccgcgggaa gacaggtaat aattgctact ttatatggat atgcaaccaa gagtgaagct
      181 gtaaatatcc caagagaggg tatttttgaa ttaactgttg aactttctcc tcttttagga
      241 aaattaatgg gatatgtgtt ttccgaagat ggaaaaccaa tttctggggc agaaattacc
      301 ttaaatgggg attatcatac tacctcagga gctgatggaa gctttgtatt atctaatttg
      361 ccagtgggga agtttgtcat tactgtagaa aaagttggat tcttacctta ctcggatgaa
      421 gtagaaattg tagctaactc tattaccact ttagagataa ctcttaatca tcttacttcg
      481 aaatgaagaa tcgtgttaaa atggagaaaa agggggaagg aggatagaga aatttgggag
      541 atacttttga tgtcagtcat tatttgggag tgtttttaga agaggctaaa gaacatatcg
      601 agagcttaga ggaaaatctg gtagagctag aaaaagaatc tgataatcag gaccttattc
      661 aggctatttt tcgttctttt cacactatta aaggctcttc tggctctatg ggttttcaac
      721 aaatggcgga attggctcac ctcttagaga gccttttgga taaggtgcgt cggggggaag
      781 tatctgttag tccttctctg attgaccttc ttttgcaagg agtagacttc ttaaagagga
      841 tgctggaaga agtagcagag aaaagacaag agccagattg ggatatgaag gaaatatttt
      901 ctgcattaca gaattttcga gaagaaagag aagaagagag taacgaggaa tactggttga
      961 cagttaaatt ggcttctgac tgctctatga aaggggtgag agcctatctt gtaatcagtc
     1021 gtctgcaaga tagcggaata ggagtggtga gtactgttcc ctctctttct gacctcgaag
     1081 gagagaattt tggtcagagc tttgatgttt tagttcatac tactctttcc ccgggagagg
     1141 ttgctaaaat tgtggagtct atagctgaaa tagagagtgt ggaggtaaaa ggagaagaag
     1201 aaaataacca gatagaagaa aaagaagaga aagaagaaaa aaatgctatt ttctctcctg
     1261 gagagcagag aattactgct aatttgaaaa agacggtgag agtagatgta gagagattag
     1321 attctctgct caacctggta ggagaattag taattgatcg tactagattg gaagatgtga
     1381 ttcagcgctt gaaagaatat caagaactgg ttcagtttcg agaaatacta tctgatgcca
     1441 ccactcatat tggccgtatc accaatgatt tgcagatgga aataatgaaa gccaggatgc
     1501 ttcctctggc tgaggttttt aaccgttttc ctcgtttaat aagagacctt gcccgtcagt
     1561 caggtaaaga aataaatttt atcatcgagg gagaagaaac agaattagac cgctctcttt
     1621 tagaagaaat aaccgatccc ttgattcatc tcttgcgtaa tgctgtggat catggtatcg
     1681 aaagtagaga agaaaggata aataagggta aaccctccca aggtaaagtt gtccttcggg
     1741 cttaccagga ggaaaactct gtagttattg tggttgaaga tgatggaaga ggactggata
     1801 tagaaaaaat aagggacaaa gcagtgaaaa tgggcctggt taccccggaa gaagcgggta
     1861 aaatgaccga gcgagaaata gtagatttca ttttctaccc cggtttctct accagcgata
     1921 aggttaccga agtttctgga agaggggtag gtatggatgt ggtaaaaaga aatatcgagc
     1981 ggataaatgg gcaggtgaga gtagagagcc agctggatgc aggaactact ttttatcttc
     2041 gcttacctct gaccctggct attattagag cgttacttgt aagggtagga gaatccatat
     2101 attctatacc tttatctgat gtggtagaaa tagaaaaagt ggagaagaaa aatacttact
     2161 gggtaaatag ggtcttaact accctttttc ggggaaaaac tcttcctctt ttatggttag
     2221 gagctctact ggatttccag agatacgatt tcataactga agacgaaata cctgaaattt
     2281 tatatactgt agtggtcaat gcttcccata gtctggctgg tttggtggtt gaggaggtac
     2341 tgggagaaca ggagatcgta attaaatctt taggttccta tataggagat gtgcgaggtc
     2401 tgggtggcgc cacaattctt ggtgatggag ggatatcgtt gattatagat gtagccagtt
     2461 tgttatggaa attttcccaa gcaggaggtt agcaaattat gccaggaaga ataatgatag
     2521 ttgatgatgc tgcttttatg agaatgatgc tcaaagatat tctgactaaa aatggttatg
     2581 aggtggtagc agaagcaagc aatggtcaag aagcattatt aaaatatgaa gaagctaaac
     2641 ccgatcttgt aactatggat attactatgc ctgaaatgga tgggattact gcagtaaagg
     2701 aaatcagaaa gaaagaccct caagctaaaa ttattatgtg cagtgctatg gggcagcaag
     2761 ctatggtggt agacgccatt caagcaggag cacgagactt tatagtgaag ccttttaagc
     2821 ctgaccgagt tattgaagca gttaaaaaag ttttaggaaa ttgaaagtta aatcttggtg
     2881 atgaaaaaga tttcttctct ttcttctttt ctttgtcaga tccccttagg ggtaattata
     2941 tttacccggc gaggcagggt aatgtattgg aataaagaaa tggagaaaat aactggccgg
     3001 gataagaagg aagctataaa aaaggaagta aaggaaattt tgggttttga tttttcttgg
     3061 gaagaaattc aaagaagttt agagtttgag gaagaatttt ttattcgtaa ttggccgata
     3121 aaggatcatt ttgaagagga acacttatta aatcttcatt tcttcttatg gaagaaagag
     3181 ggtaaaaaaa cgaatgtaat gtgtctggtg gaggatgtaa cccagaaagc ccggtggggt
     3241 gaaaatatcc ggcaaatgga aaaattatcc agtattagta ggttcattgc cagtatggct
     3301 catgaaatca ataaccccct gacggttatt tctggctata cccagatgtt gctgcaaaaa
     3361 gcaaaagaga acagacaatg tgaggtttgt cgagccaccc aacgagggct ggaaaaaatt
     3421 gaaaaagagg ctgagtattg tggaagattg attcaggagc tggtagattt ttctaagccc
     3481 atgattctta ataaagaaga cattgatctt aacttcttgg tgcaagatag tctctcagta
     3541 ctggatgtgt tgatggatga caaaataaag ctggagctga actttagtaa tgatgtactt
     3601 ttggtgagag tagataaaag caggatgaga caggtattta tgaacctggc taaaaatgct
     3661 ctggaggcta tgaaagaaga gggaggaaaa ttgcaaatta atactcgtat tgcccctttg
     3721 gagcattttt cttttcctga gaatggaaca ggaaacggta gatttgtaga gatagaattt
     3781 attgatcaag ggaaaggtat accagaaaag acgctacctc acatatttga gccctttttt
     3841 actactaagg aaaaagggac cggtttgggt ctttccatct cctatagtat tgtgcgtgct
     3901 cataaaggat ggatagaggt aagctctcaa gaaggagggg ggacaagaat aaaagtgttt
     3961 cttcctcagg gaggaggaaa ttgatatgga actaagagct gattttcgtc ttgttcagcg
     4021 tcctcagctt attcccaaaa tggctcaagc agtggagatg atggctctac ctcttcctca
     4081 aatagaacaa aaattgcaaa aattaataga agaaaatcct cttctggagg taagagaagg
     4141 aagcgtttgc tataactgtg ggaagagctt tagtggttat ccagagcgat gcccttactg
     4201 tggaagatac ctcagaagaa gggagaagga ggaattttca ggagaagaat ttcctcaatg
     4261 gaaagaaaag aaagactgga aagaaatgat gaaagaagaa attttttcct tcatagagtt
     4321 gtgccctaag gaaaaaaggg ttcttcactt tctgattgct cggttagatg gagaaggatt
     4381 tctgcgggaa gatctggaga aattatcttt tttactagga gaagaagaca atctggaaaa
     4441 aataattgaa aaagtgagaa aggaaggatt tctgggtttt gctgctcgag atgccataga
     4501 ttttctgatt ctacagatga tagagttagg gttgataaag aaagaggaga aagataaggt
     4561 gcgtcgagaa cttctttctc atcctcataa gtaccaaaaa ctcctggaac cttatcagga
     4621 aaaactcttt ttttctccgg tgcaattttt tgaaacttct ctgggtcgag aggcgggaga
     4681 attggaatct tctctttctt ttcttatccc tgatgcagaa atcctggagg taggagaaaa
     4741 tagttttgag gtgcttctca ctccttctct ttattttcaa ttggtgataa atgaagaagt
     4801 actctctttt taccggaaac ggaggaagga gactcccgaa cgagaaaaaa ttttctggca
     4861 agaaaagctg ggagaagcgc gagaggtaat atcttgtctc aactatcgga atcaattgtt
     4921 gttgcgggtt ctagactata tagtagaaag agaaaaggat tttttgcttc atggctttcg
     4981 ttactttgtt cctcttactc agagggaaat agctgaagga gtaggagtga gtataagcgc
     5041 ggtttgtcag gtattgaaag aaaaatcggt ctgttttccg gacggggtaa caaggagcgt
     5101 aaaatttttc tttgataatt cttacccggt taaggaaatg atgaaaatta tcttgactcg
     5161 agaaacatcc catcatcctc tttctgatca ggaaataatg aaggaattag aaaaaaaggg
     5221 tttccgcata gcacgtcgga cctgtgctct ttaccgagaa gagatgggcg ttttaccttc
     5281 ttacttgcgc aaaagactga aggaaaaata atttttttta aactcttccc tatgaccttc
     5341 ttcccccccc cttggcatga taatttatcc tcaatggttg aaagtaaaaa tttgaggtgt
     5401 aaagaatgag aaacgagatt attcaggatc cggtttttag acttctggaa agaggtttgg
     5461 atttatcttt agcccggcaa agtttgttga taaataacgt ggctaatacc gaaacccctg
     5521 gttaccagcg tcaggattta aattttgaag cctgtttgaa agaaactgaa aaaaaggggg
     5581 atttacaatt aactcacccc aatcatctgg cgccggggaa aggagaagga acaattactg
     5641 aaggagtaga gcaatattca gtgcgtcaag atgggggagg agtggacatg gagaaagaga
     5701 tgactttgat tctggagaac gccctttact atcaaacttt gacccgtgtg gtttcaaaaa
     5761 agcttggttt attggggaca gtaatcaggg aggtgcgatg agatgaaaat attccgtaat
     5821 ttcgatgtca gtgcttcggc tcttactgct gaacgtttgc gaatggatgt tatatcttct
     5881 aacgtggcta atgttgatac tactcgaaca caagatggag gaccataccg aagaaaaatg
     5941 gtggtatttg aggtggcagg agatggagga gtgagagtaa ataaaataca ggaagatttt
     6001 tccactcctt tgcaattagt ttaccagcct gggcatcctg atgccaatgc cgatggatgg
     6061 gtagagtatc ccaatgtggt tttagtaaat gaaatggtgg atatgatggc tgccacccga
     6121 gcttatgaag ctaacgtagc agcactaaac gctactaaaa gtatgatgag cagtgctctc
     6181 aatattggtc gggaatgagg gatgaaaaaa tgagaataga aagcaatgta ccttctcttt
     6241 ttctgggtct ggaaggggaa cagccttctg gtacttctcg ggaaattggt ggatttggag
     6301 aatgtttaaa tcgagcttta caaaaagtaa acaatttgca gattgaagca gaccagatgg
     6361 cagagaaggt agcacgggga gaagaagata tccataatgc tcttatagct ttggaaaaag
     6421 ctaatttagc cctagggctt acggtgcagg tgaggaataa agttgtagaa gcttatcaag
     6481 aaatcatgca tatgcaggtg taggtaagtt atgaaaaacc ctttaactga gtttgctgaa
     6541 cgggccaaaa aaacttggga agggctcaac cagggacagc ggttgttgtt tttgcttatt
     6601 tccaccctct tggtcgcagg gatagttact cttttccttt atgccggaaa acctgactat
     6661 gctcctctgt ttcaaaatct atcagtggaa gaagcaggga aagtgatgga taaattgaaa
     6721 gagctgggta ttccttatca gctttctagt ggaggaacca gaatcctggt tcctcaggat
     6781 aaagtttacg aagcgcgaat gaagttagct caagaggggt taccttctca gggagagggt
     6841 tttgaaattt ttgataaaaa cacttttggt cttaccagct ttttacagag agttaattat
     6901 caacgggctt tgcaggggga gctggaaaga accataatgg aacttaaaga ggtagaagga
     6961 gcccgggtgc acctcgtggt tccggaagag cgactttttg ctgaagagga aaaaccgcct
     7021 actgcttcgg tagttctgaa gcttcgctcc ggagttaccc tgcaggatgc ccaaatagag
     7081 gctatcgcta atttaatagc taatagtgta gaaggcttga aacctgaccg ggtagctatc
     7141 atcgatgatc ggggaaacat tttagctagc ggcagcggag atgaatccag ttggacgagt
     7201 ttatctcgtc ttactgctac tcaattagag gtgaaaaagg aagtagaggc aactcttacc
     7261 aacagaattc aatctatgtt agaaagtgct ctgggatata atcaggcggt ggtgcgagtg
     7321 actgctgatc tggattttga acaacgggag ggtcagaagg aaacttttga gccgataagg
     7381 ggaggagaag ggatagcaag gagtgctcag gaaacagaag aaaaatatca gggaggagtt
     7441 acagcaccag gaggagttcc gggtgtggct tctaatattc ctgtttatgg ccaagagagc
     7501 gaagcaacag gacaaactaa tgattatacc cggcgggagt cgacggtaaa ctatgagatt
     7561 aaccgggtta tggaaaggtt tgcttcttct ccggggaaag ttaccaggct ttcggtagca
     7621 gttttagtag acaataacct tccttctgag caagtggaaa agatagaagg ggtagttaag
     7681 gcagcagcgg gtttgaacga agaacgagga gatatgttaa tcgtggaatc tcttcctttc
     7741 aatagagagg cacaagaaga agaaaagaaa gctttggctt cccagaatat gatgaagtgg
     7801 atagaacttc tggctaaata tggattgata gggattttaa tttttgtgat gtattctctg
     7861 ggaagaagga tactggtttc tgtcgctgtt cccgaaaaag tgaccgcaga attttatgaa
     7921 gagccagaga tggaatatga agaagaaaaa ttacctccta ttaaaccagt ggaactcagc
     7981 cctgaagaaa aagtaaaaat ggaagtgcaa aaaagaatgg aagaagatat taaaaaatta
     8041 gtgcacacca gtccagaaga cgcggttaaa cttttaagaa gctggttgca cgaggattag
     8101 cttatggcta tcgttcaacg taagtcactt actggcagac aaaaggcagc catttttctt
     8161 gtgagcctgg ggccggacct tgctgctcaa gttctaaaaa ggctcactga tgaagagata
     8221 gaagatatta ctctggaaat agctaccctg gaaaaagtcc ctccggagct gcgagaagaa
     8281 gtgattggtg aattttatca cctggcttta gctcagcagt atatttctca ggggggtata
     8341 gattatgccc ggcaacttct ggagaaagct gtaggtcctc ataaggctca agagattatt
     8401 agccggctct ctgccacttt agagattact ccttttgaat ctttacgccg ggctgacccg
     8461 ctgcaactgg tgaatttcat tcaggacgag catcctcaaa ctattgcttt aattctctcc
     8521 tatttaaaac cagaacaggc ggcaatggtt ttaggaaatc ttcccgaaag cgtgcgggga
     8581 gaagtagcca tgagagtagc tcttatggat cgaacctctc cagaagtagt taaggaaatt
     8641 gaggccgttt tagaaaggaa aatttctaat tttcttacta ctcaggattt tactcgggtg
     8701 ggagggaaga aaaaactggt agagattctg aacaaaagtg atagaggaac agaaaaaagt
     8761 attatagaag ctctggagga gagagacccg gaactggctg aagaaattaa gtcggagatg
     8821 tttgtctttg aagacatcat aactctggat gatcgagcga tacaactggt gttgaggcag
     8881 gtagatatga aagatttagc tctggcttta aaaggtgctt ctccagaatt gcaggataag
     8941 attttccaca atgtttctca aagagcggca cagatgatca gggaagatat ggagtatatg
     9001 ggtccggtga gagcccgggt ggttaacgaa gcgcaacaga aaattgtcaa tgtggtccgc
     9061 catctagaag aagcaggtga aattgtaatt gctcgtggcg ggggggagga tgaaatcttt
     9121 gtttaggctt tacaaagaag ttaagtttct tcctgatcta ttcctggtag gagaagagga
     9181 taataatgat gggaaagtta tagtaaagca agaagaaaag gaggataacg aggataaatt
     9241 tcaggaagaa aaaagtctta ttattgaaga agcgaaaaga aaagctcaaa tgatcctaga
     9301 agaagcggaa tctgagagga aaaatataga agataaggcc tgtagagaag gccgagaaga
     9361 gggtagaaga ataggaaaag aagaagtaca gaaacaatgg caagaaaaag tagttgtttg
     9421 ggaacgagag ctccgggaga tttataacta tcgagaagaa atgatagaaa agatgaaaga
     9481 gccccttcta gaacttagtt ttatcatcgc tcggaaaatt atagaaagag aagcagaacg
     9541 ggaaccattt attgaacgcc tggtagagaa ggctttgaaa aagttgagca atcgggaacg
     9601 agtagttttg cgggtgagcg aagaagaata taacctggtt cgggaaataa gagaagaatt
     9661 aatgagaaag atagacggga tagactatct ggagattcaa gaagatcgtc gcctgaaaag
     9721 aggggaatgt ctggtagaaa cttcttttgg tagtatagat gcgcggatag atactcagct
     9781 ggaaaacatc aaagaggagt tgtttcaagc attagaggag ggaactacaa gtgcttgatt
     9841 tttctagagt aaaaagaata gttgaggata ttgacccttt acgcttgaat ggaaaagttc
     9901 tgcaatcagt gggaatagtg atggaatctc aaggtccttc ctgccgggta ggggagttat
     9961 gccgagtgcg ttctctggat agagaaaatg aaatattggc tgaagtagtg ggctttcgag
    10021 gagaaaggac tttacttatg cctttggggg aaagaaatgg aattgaatta gggagtgaag
    10081 ttatagcctt gggaaaggaa atgaaaatca aggtggggga agatttgctg ggacgggtat
    10141 tggatgctct gggtcgccct atggatggta aaggtcctgt agagggagaa accgaatatc
    10201 ctctggattg tgagccacct cctcctttag atagaaaacc tattagagag gttttacctc
    10261 taggaattaa agctatagat ggagtactta cctgtggaaa agggcagaga gtgggtattt
    10321 tcgccgggag tggagtggga aaaagcactc ttttgggaat gattgctcgt tatgctcaaa
    10381 gtgatgttaa cgttatagcc ctcgtgggag aaagaggaag agaagtaaaa gaatttattg
    10441 aaaaagactt gggagaagaa ggattacgtc gaagttgtct tgtagtagct acctcagata
    10501 aagctcctct tttacgtatt aaagctgctt tttgtgctac tgctattgct gaatatttta
    10561 gagataaagg gaaagatgtt cttttcctta tggattcagt tacgcggctg gctatggctc
    10621 agagagagtt aggtctggca gtgggagaac cacctactac cagaggttat accccttcag
    10681 tgtttacttt gcttccccgg ttaatggaaa gaacgggaaa ctcacggcga ggaagcatta
    10741 ctgcccttta tacggtgtta gtagaagggg atgatatgaa tgagcccatt gctgatgcag
    10801 taagagctat tttggatgga catattgttc tttcccgtaa actatctttt gagggacatt
    10861 acccttctat agatgtttta caaagcatca gccgtcttat gcctgatatt actgacgaaa
    10921 agcaacagca aatggctcag aaattaaggg aaattttggc tacttacagt gaagcagaag
    10981 atttaataaa tattggtgct tatgaacagg gttctaatcc tcgtattgat tacgctttaa
    11041 cgatgataga tagagtgaag aagttcttac gccaggaagt aggggaaagt ttttcctggg
    11101 aagaaacaca ggcttttctt tcccagcttt ttgaaggaga aaagtaaatg aagggatatc
    11161 agtttaagtt gcagaaagtt ttagatgttc gtaaagttaa agaggactta attggagccc
    11221 gagtagcaga actggaagag ataattgaag ctcaaaaagg acaccttctt tctttagaga
    11281 agaaaaaaga gggggttctg gtggaacggc gggaaaagag agagatagat aatggcttca
    11341 ggattgaagg agaaatgttt tatgaatggt atttagaaaa cttagaaaga aaagtagagg
    11401 tagttaaaac ttcgctgaag cagctggaag agcaaatgcg taaaaccaga gaagaattaa
    11461 tcaaggcagc agtggacagg aagataatgg aaaaattgaa agataaggac tatgagaatt
    11521 ttaaagtaga agtaattaga aacgaccaga aaaatttgga tgaaattact atccagagat
    11581 ataaaagggg gatagaataa aatgcttcct tataatctgg aaaaaattat ctctcgaata
    11641 ggggagatag agagcagatt ttctcaacga gtttcttcgt tggaagaaag aacaattaga
    11701 ggcgatgata actatgctgc ttctcaagat acctttgatg atttgataaa agaatacgcc
    11761 caaaagtacc aaatagacca tcgtttggca aagaggttga tcgaagcgga atctaacttt
    11821 gatcctgatg ctgtctctcc tcgaggggct atggggttga tgcaacttat gccccagact
    11881 tgtcaggatt tggggataaa agaccctttt gatgagcggg aaaacttaga agggggaatg
    11941 agatatctac gaggtttgtt gaaccaattt ggtagtcttc ctctggctct cgctgcttat
    12001 aacgccggtc cttctcgagt gaaggaatat ggagacatac ctccatttcc ggaaactcaa
    12061 gagtttgtaa agaaagtgat aggaggataa tgtagccggt ggccagtaga gagatggata
    12121 tccagaaaag aaaagaaaaa acagccaaca ataaaacttc gaagaagaag aagagtaact
    12181 ggatagagac gatattgccc gttctggttt ttgcctttac tctggttttc tttctcaatt
    12241 tttcaggggt tattgatttg cccttcttgc attttatgcg cctaccctcg gtggaaaaag
    12301 aagattcaga aaatttaatg gtaaaagaag tagaaaaaga aactgaaagt caagttgttt
    12361 atcctggtcc tactttagaa agagtagaac ccgaggagag ccctgtgata gaagagcctt
    12421 tagaagaaga ggtagaggta ttgcctgaaa tagaagagcc ttcttcgggt aaaacagatg
    12481 gagaagatgt cgattcttct gctcgagaag aaaggatttt tcgtttggct aatatttaca
    12541 ccgctatgga gccggaggaa gcatcccgga tcatggaaaa actttctgat gaggaggtgg
    12601 tggaaatact ttcggttatg aaagaaagaa atgtagccga aatattaaat gcttttccgg
    12661 tagaaagagc agcagagatt atgaaaaaaa tgatggaagg gaggtgaaaa aaatggatgg
    12721 ttttatattc ttaccggtag aaagtggaga gatgaaaggt aatagcttta ctgtcgataa
    12781 gatagaaaaa gaggaaggaa aagagagtgt aggctttgaa caacttttat ctccttttct
    12841 attctcctgg gggacaggag aagttacgcc ggtaaaaagt tgcagttctt cctcttcggt
    12901 aagtaactgt tctttggagc gttctacttg ttctccctgt tctctctctg gagcagatac
    12961 tttttcctct ggagcttgtt cgttgtccag ttcctcttcc ttagaggaaa tgttgaatgt
    13021 agagggatgg caaaagacaa tagatagctt tctctcggag gtagaaaata ttagctttac
    13081 tctgaactta ggagagaaag gaaaagtatc agtgagcggt ggaacgggag aagatggaaa
    13141 atttactttt caaatctctg gagacaagga agccctcagt gagctattgg gtagagtagt
    13201 aaagcaagtt gttgctttct taagtgaaca agggttttct tatcctgggg gagaaagttc
    13261 tttctctgag tgttccctgg gacaggggtg tggaggagga tgtggtgggg aagagataat
    13321 cacttcccaa attggggaga ttcaaggaga cacagagttg ggatacatta ttcctgatga
    13381 tttgcttcct cccgaggaag gagaagaaaa cgctattttc tctgatgttt ctcttttacc
    13441 tgatgtcgaa gtagaag
//
LOCUS       DAOSVE010000044        13202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_2797_length_13202_cov_68.507873, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000044 DAOSVE010000000
VERSION     DAOSVE010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 13202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13202
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2797_length_13202_cov_68.507873"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="PK016_05065"
     CDS             <1..1005
                     /locus_tag="PK016_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HXL02472.1"
                     /translation="VPREKIVSVSIMPCTAKKFEARRSEFIQDEIPDVDLVLTAQETI
                     RMIKTANIDFKELDPLPFDIPLGAGSGAGVIFGVTGGVTEAVLRYAYERLTGQELKEV
                     EFKGVRGMESVREAEVDFGERKVKVAIVHGLANAQKLIKDIRSGERYYDFVEVMNCPG
                     GCIGGGGMPLEYPDYQEVLRRRAEGLYNADRLSVVRKAQDNPSIKFLYEKWLKEANSE
                     VAEHHLHTHYRSRRRISDEMIRIQEAKSAQKVDIAVCVGTSCYLKGSYDLLQELLELT
                     KEYGLEDRVSVGATFCLERCTEGPNIRINEEIFSGVSKDNLRQIFEEAVLSKLVAETH
                     "
     gene            1053..1128
                     /locus_tag="PK016_05070"
     tRNA            1053..1128
                     /locus_tag="PK016_05070"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1086..1088,aa:Ala,seq:ggc)
     gene            1165..3639
                     /gene="leuS"
                     /locus_tag="PK016_05075"
     CDS             1165..3639
                     /gene="leuS"
                     /locus_tag="PK016_05075"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HXL02473.1"
                     /translation="MGGLEVLQDYDFTEIELKWQKIWKEKKIFEVERDEDKEKYYVLE
                     MFPYPSGDPHMGHVKNYVIGDVVARYFMRQGYNVLHPMGFDAFGLPAENAAIQHHIHP
                     AVWTQDKINRMREILKRLGISYDWRREVITCEPDYYRFTQWFFLLFYKNGLAYKKEGS
                     VNWCPSCSTVLANEQVVDGACERCGTPVIRKKLEQWYFRITRYAEDLLKDMALLGSWP
                     EKVLTMQKNWIGKSEGAYIDFVLNDGEKVTVFTTRPDTIYGVTFFVLAPEHPLVDKLV
                     AGTPYEEKVKEFREKIARKSEVERLSDIAEKEGMYIGQNIINPVNREKIPIWIADYVL
                     LEYGTGAVMGVPAHDERDYQFAQKYGLPVKPVIKKVDGDEYCLTEPGIMVNSGPFSGL
                     PSEEGKKKVVEFLEEKRIGKGAVSYRLRDWLISRQRYWGAPIPIIYCERCGEVPVPEE
                     DLPVLLPRDVDFLPGGPSPLARSLEFVNTTCPRCGGKAKRETDTMDTFVCSSWYYLRY
                     TSPEAKDKPFCKKDVDYWMPVDQYIGGVEHAILHLLYSRFFTKVLNDLGLVNFREPFS
                     NLFAQGMVTKGGVKMSKSKGNTVSPREIINRFGADTVRVFILFAGPPEADMEWSDQGV
                     EGAFRFLSRVWKAIGGIIDLPVQEVEPDKLKREALERKMHQTIFRVTRDIQEKFHFNT
                     AISSLMELLNEMSDYLRFLEEKGANSEEKEMLVSASRTLVSLLNPIAPHISEELWRLL
                     GEKSLLSLESWPAYQEEKLYEEVIEVIVQINGKVRSKVVVPRGSSEEEVLAQALQDEK
                     VKSWLNHGEIKKSIYVPDKLINLVVG"
     gene            3641..4186
                     /locus_tag="PK016_05080"
     CDS             3641..4186
                     /locus_tag="PK016_05080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HXL02474.1"
                     /translation="MDFSRQEKLALLIISLVVALGLGFITWRNFSTPEVGEETLLVIQ
                     VDGAVNSPGIYRVKPGTRIFEALELAGGTREDAQTEGLNLALPVYDGQRVSIPSTTIS
                     PSLKEEAISPQVQPALQLVNVNTASQKELESLPGIGEVIAQRIIEYREQNGAFSRPED
                     LLKVKGIGEKKLEAIKNLITF"
     gene            4855..6714
                     /locus_tag="PK016_05085"
     CDS             4855..6714
                     /locus_tag="PK016_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL02475.1"
                     /translation="MIGFTQIGFAQLSEDTPREETLITDVLGGRARNPGKFNAFVATG
                     ITSDRGIQQLMLEPLWTIEPATGEVINSLAAEGPIYNEDFTQMTVKLREGCYWSDGVP
                     ITADDIVYGIEMAMKHPGMAYHDQLNLYVDKVYKTDDYTVVFELKGPNGRFHTNFLDR
                     WGAWRPSPKHIFEKVEDPLAFDFNPPISSGPYVLKDYDQAGYWTLWERREDWDKTPTG
                     MLYGMPKPKYVLFYYYGDATNKVIAQANHNLDSVDLTLEAFRALLEQNKYSRGYRKEY
                     PWVVNIDPCMTGLMFNNDVSPYDLKDVRWALTLAIDIVDHIGIAFDGAAAMGALHIPP
                     MPVYQKWYYEPMEEWLKNFTLDIEVNGEPFKPYDPDAPFRLVEYAKSRGYTVPEDPEE
                     IREAFGPGWWKYAPDVAEQLLLRNGFTRDVDGKWLLPDGAPWKITILTSTNPAQPQHR
                     NALAAAQQWRNFGIDVTVNTNEAVNDLVFRGDFEISTNWPANEPWGGHPDLYRTVSIF
                     HSKYYKPIGEIAVSSNGAASRWKDPRMDEVIEGMEKVDWDSEENLRLGMEGLKILVEE
                     MPTLPTFGYPGVLAWDEYYWTNWPGAENPYVIPYHHWPNLKYMLPFLEPAGNK"
     gene            7502..7735
                     /locus_tag="PK016_05090"
     CDS             7502..7735
                     /locus_tag="PK016_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02476.1"
                     /translation="MIVKIGARSHQALKVQECLTKYGCNIKTRLGLHEASPEYCAEDG
                     LIILELTGKKEENEQLKKELESLEGVTVQYLEI"
     gene            complement(7710..8696)
                     /locus_tag="PK016_05095"
     CDS             complement(7710..8696)
                     /locus_tag="PK016_05095"
                     /inference="COORDINATES: protein motif:HMM:NF027889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp1/GlmU family protein"
                     /protein_id="HXL02477.1"
                     /translation="MFWPPEWDFLPHALNNFRGILMVVAPPDRGKTTLIRLLSSFFVS
                     QGKKVGWVDSDLGQSNVGPPATVGMVMVSKKFPLSLRERLFTPYFRFVGDVTPEKRIE
                     ATALYTAELTKMASSKTQITLIDTCGLIFSLSGLRLKMLKMKLIKPELVLGIGRDEEF
                     EVLKKFYGEQFWVVSPSSRASKKDYEKRRSHRERLFSLYFSSGENWEMGWENLRFFSP
                     YHPYFFLQEYHLREGIPTFEFSNNFFLILSTSPEKKGDNKCRVINPSSLSFSICGLYT
                     ERGEELGLGVIQSINEEKKTIAIRGTKINPGKVEVLVPGVTKINAKSPDIEQ"
     gene            complement(8706..9410)
                     /locus_tag="PK016_05100"
     CDS             complement(8706..9410)
                     /locus_tag="PK016_05100"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXL02478.1"
                     /translation="MHKLKVWLWVSCFILLIFLPREAKGIDIETEENVFGLNLVRSLG
                     GNSQVRKEVALTFDDAPSPFTKEVLKILDKHGVPATFFLIGSQVEKSPEVARQIVAAG
                     HEIGNHSFSHRWSSDDSVEALVKDIKRGEEAIYRATGQIPLYFRPPGGMVNGNVKEAC
                     GQTGYSILLWWVDSQDWLLTDEEEILETVKKEVRDGAIILFHNLATTVKVLPQVIENL
                     KKEGYKFVTVSQLLRY"
     gene            complement(9410..9739)
                     /gene="trxA"
                     /locus_tag="PK016_05105"
     CDS             complement(9410..9739)
                     /gene="trxA"
                     /locus_tag="PK016_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015222351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HXL02479.1"
                     /translation="MRNMIELNKDNFKEEVLDSEIPVLVDFWAVWCMPCRMMAPIVED
                     LANSWKGKVKVGKLNTDEAPEIAAKYGIQAIPTLLIFQGGKEVGRIVGYVPKKTIEEK
                     LQSIIKS"
     gene            complement(9736..10329)
                     /gene="plsY"
                     /locus_tag="PK016_05110"
     CDS             complement(9736..10329)
                     /gene="plsY"
                     /locus_tag="PK016_05110"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HXL02480.1"
                     /translation="MVNWWVLPLNYLLGSFPCGLIVGWIAKGVDIRKYGSGSIGATNV
                     SRVAGFLPALIAGVGDALKGFLGAYLATHFLSNPYLWFLALFLLILGHDWSIFLRFRG
                     GKGVATTLGILFYLSWESAILCFLAWIIMASITHYSSLGSLSGALLMPIFLYLFGKPP
                     EFIIWGIFAAALVFWRHKGNIQRLIKGKELKMGQKGA"
     gene            complement(10335..11576)
                     /gene="fabF"
                     /locus_tag="PK016_05115"
     CDS             complement(10335..11576)
                     /gene="fabF"
                     /locus_tag="PK016_05115"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HXL02481.1"
                     /translation="MERKVVVTGIGVISPVGSGKDKFWQSIIGGKSGIDRIKSFDPGD
                     FDSQVAGEVKDFNPEDFLPRKEYRRMDRFSQFAVCASLMAMEDASMSLENLDRERAGV
                     ILGSGIGGIQTFEDQHQVLLTKGPGKVSAFFIPMMIVNMGAANVAIQLKLKGPNSCVS
                     TACEASTHALGEALRIIQRGEADLMVAVGAEASITPLALAGFCAMKALSTHNDDPAKA
                     SRPFDRTRDGFVMAEGAAALILEEEERAKKRGARIYAELKGYGSTCDAYHITAPDPEG
                     EGAARSMKLAIEDACLTPEDIDYINAHGTSTPLNDKVETLAIKKVFGERAYQIKISST
                     KSMTGHLLGGAGALEGAITALSIYHGIIPPTINLEEKDPECDLDYTPDHAFSLKIRNA
                     LSNSFGFGGHNGSLVFSRYGE"
     gene            complement(11590..12573)
                     /locus_tag="PK016_05120"
     CDS             complement(11590..12573)
                     /locus_tag="PK016_05120"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HXL02482.1"
                     /translation="MSEGKVKILGTGSSVPERVITNFDLEKIVDTSDEWITTRTGIKE
                     RRIAQKEESTSTFALLAAQRALQAAQVSAEELDLIVVATVTPDMLFPATACLLQRELK
                     ARKAASFDLEAGCTGFVYALSLAEKYLRGGGGEKALVVGAETLSKILDWQDRSTCVLF
                     GDGAGAAVLGLSKEGGIIATYLGSDGGGAHLLELPAGCSRMPASLDTVNNRLHYIKMN
                     GNEVFKFAVKIMEEASLEVIKRGHVEMDEVNLFIPHQANIRIINSAAKRLGIPEERVF
                     VNVHKYGNTSSASIPLALDEAYREGKINGGDLVLLVGFGAGLTWGSALIRW"
     gene            complement(12570..13010)
                     /gene="fabZ"
                     /locus_tag="PK016_05125"
     CDS             complement(12570..13010)
                     /gene="fabZ"
                     /locus_tag="PK016_05125"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HXL02483.1"
                     /translation="MELDVKKIQEILPHRFPFLLVDKIISLEDDKVVGLKNVTMDEWF
                     FQGHFPGKPIMPGVLILESMAQVGATMMMNMEEFRGCIPYFTSLDGVRFRRPVMPGDQ
                     LVMEVTLLKVRRGIGKLKGIARVNEAVVAEGEIGFSLVKEEAKD"
BASE COUNT         3864 a         2742 c         2851 g         3745 t
ORIGIN      
        1 gttcctcggg agaaaatagt ttctgtttcc ataatgccct gcacggctaa gaaatttgaa
       61 gctcggcgtt cagagttcat tcaagatgaa attcctgatg tagacctagt tttgactgct
      121 caggaaacca tcagaatgat aaaaactgcc aatattgatt ttaaagaatt agaccccctt
      181 ccctttgaca ttcctctggg agcaggaagt ggagcaggag ttatctttgg agtaacagga
      241 ggagtaacgg aagcagtgtt aagatacgcc tacgagcggc tgaccggcca ggaacttaaa
      301 gaagtagaat ttaagggagt aagaggcatg gagagtgtaa gagaagcaga agtagatttt
      361 ggagaaagga aagtcaaagt agccatcgtt cacggtttag ctaatgctca gaagttaata
      421 aaagatataa gaagtggcga gagatattat gactttgtgg aagtaatgaa ttgtcccgga
      481 ggatgcatcg gaggaggagg aatgccactg gagtatcccg actaccagga ggttttacgc
      541 cgcagggcag aaggcctcta caatgctgac cgcttgagcg tggttcgtaa agctcaggat
      601 aacccctcca ttaaattctt atatgaaaag tggctgaaag aagctaacag tgaagtggca
      661 gaacatcacc ttcatactca ctatcgctct cgtcgccgaa ttagtgacga gatgattcgg
      721 attcaagaag ccaaaagcgc tcaaaaggta gatattgcag tttgtgtagg caccagttgt
      781 tatctcaaag gctcttatga cctcctacag gaactattgg aattaactaa agaatatggc
      841 ttagaagata gggtgagcgt gggagctact ttctgcttag agcggtgtac cgaaggtcct
      901 aacattcgaa ttaatgaaga gatattctcg ggggtaagta aagataacct caggcaaatt
      961 tttgaagaag cagttttaag taaattagta gctgaaactc attgacggat agaagtggtt
     1021 tgcgtataat gtatgaggtt tttaaaaggt aaggggctgt agcgcagttg ggagcgcgtc
     1081 tccttggcgt ggagaaggcc acgggttcaa ctcccgtcag ctccaccaga tgatgagctc
     1141 gcccctcggg ggcgagcttt ttttatggga ggtctggaag tgctccagga ttatgacttt
     1201 acagagatag agcttaagtg gcagaaaatc tggaaggaaa aaaagatttt tgaagtggaa
     1261 agagatgaag ataaggaaaa atactatgtt ttggagatgt ttccttatcc ctctggtgac
     1321 cctcacatgg gtcacgttaa gaactacgta attggagatg tagtagctcg ctattttatg
     1381 cgtcagggct ataatgtttt gcatcccatg ggttttgatg cttttggctt accggcggaa
     1441 aacgctgcta tccagcacca tattcatcct gctgtctgga cgcaagataa aattaatcgg
     1501 atgagagaga tattgaaacg cttgggcatc agctatgatt ggagaagaga ggtcataacc
     1561 tgcgagcctg attattaccg tttcactcag tggtttttcc ttcttttcta caaaaatggt
     1621 ctggcatata aaaaggaagg ctcagtgaat tggtgtcctt cctgttctac ggtcttagcc
     1681 aatgagcagg tagtagacgg ggcttgtgag cgctgtggaa cgcctgtaat tcgcaagaaa
     1741 cttgaacagt ggtattttcg aattacccgt tatgctgaag atttactaaa agatatggct
     1801 cttttaggga gctggccaga aaaagttttg accatgcaaa aaaactggat aggtaaaagt
     1861 gaaggggcat acattgattt tgtgctgaat gacggcgaaa aagttaccgt ttttaccacc
     1921 cgccctgata ctatttatgg tgtgactttc tttgttttgg ctcctgagca ccctttagta
     1981 gataagctgg tagccggaac tccttatgaa gaaaaagtga aagagttccg ggagaaaatt
     2041 gctcggaaaa gtgaggtaga gcgcctctct gatatcgctg aaaaggaagg aatgtatatt
     2101 ggtcagaata tcataaatcc agtaaacaga gagaaaatcc ccatctggat agctgattat
     2161 gtgctcttag aatatggaac aggagcagta atgggtgttc cagcgcatga tgaacgtgac
     2221 tatcagtttg cccagaaata tggtttacct gttaagccgg tgattaaaaa agtagatgga
     2281 gatgaatact gtctcaccga gcctggaatt atggtaaact ccgggccctt ctctggttta
     2341 ccatctgagg aagggaaaaa gaaggtcgta gagtttttgg aggaaaagag aataggtaag
     2401 ggtgcggtat cttatcgctt acgcgactgg ttgatttctc gacaacgtta ctggggagca
     2461 cctattccca taatatattg cgagcgctgt ggtgaagtgc cagttccgga agaagatttg
     2521 ccggtattgc taccccgaga tgtggatttc ctacctggtg gtccttctcc tttagctcgc
     2581 agtctggagt ttgtcaatac tacctgtccc cgttgtggag gaaaagctaa acgagagacc
     2641 gataccatgg acacttttgt ttgctcttcc tggtattatt tgcgttatac ttctccggag
     2701 gcaaaggata agccattctg caagaaagat gttgattact ggatgccagt agaccagtat
     2761 ataggaggag tagaacacgc cattcttcat ctcctctatt cccgtttctt tactaaagtg
     2821 ctcaatgact taggcctggt taattttcgg gagccatttt ctaacctttt tgcccagggt
     2881 atggttacca agggcggggt taaaatgtct aaatccaagg gaaacacagt gagcccccga
     2941 gaaattatca atcgttttgg cgctgataca gtgagagtat ttatcctctt tgccggacct
     3001 cctgaggcgg atatggagtg gtcggatcag ggagtagaag gagcttttcg cttcctaagc
     3061 cgggtatgga aagcaatagg tggaattatt gaccttcccg tgcaggaagt agaaccagat
     3121 aaactaaaaa gggaagctct ggagcgaaaa atgcatcaaa ccatcttccg ggtaactcga
     3181 gatattcagg agaagttcca ttttaatacc gctatcagtt ctttgatgga gcttttaaat
     3241 gaaatgagtg attacctgcg atttttggaa gaaaaaggag ctaattctga agaaaaagaa
     3301 atgttggttt ctgcttctcg gacccttgtc tcccttttaa accctatcgc tccccatata
     3361 agcgaagagc tctggcgact tttaggagag aaaagcttac tttctctgga gagctggcct
     3421 gcttatcagg aagaaaaatt atatgaagaa gtaatagaag taatagttca gattaatggt
     3481 aaggtgcgga gtaaggtagt agttcctcgg ggttcatcag aagaagaagt tttagctcag
     3541 gctttgcaag atgagaaagt gaaaagctgg ctcaatcatg gagaaataaa aaagagcatc
     3601 tatgtgccgg ataagttgat taatttggtg gtaggatgac gtggactttt cccgccagga
     3661 gaaattagca cttttaataa tttctttggt ggtagcttta gggctgggtt tcataacctg
     3721 gagaaatttt tctactcccg aggtaggcga agaaacatta cttgttattc aagtggatgg
     3781 agcggtaaac tctccgggta tttatcgggt caaacccgga actcgtattt ttgaagctct
     3841 ggagctggcc ggaggaacta gagaagatgc tcaaaccgag ggtctgaatt tagctcttcc
     3901 cgtttatgat ggacagagag tatccattcc ttctactact atttctcctt ctctaaaaga
     3961 ggaggctatc tctccccagg tacagccagc ccttcagctg gtgaatgtta atactgcttc
     4021 tcaaaaagag ttagaatctt tacccgggat cggtgaagtg attgcccagc gcatcataga
     4081 ataccgggaa cagaatggcg ctttttctcg tcctgaagac cttcttaaag tgaaaggtat
     4141 aggggaaaag aaactagagg ccataaaaaa tttgattact ttttagtttt aattccccat
     4201 atagagggca acttgagggg tatctcttag tccctgagga aagaacctct aatgcaaata
     4261 tatttcttca taagtggaag atattttctc tttagaattt tccaccagat ttactttatt
     4321 gcaataacta tccgcttttt attaagaata atggattttt agaatctata tctatttact
     4381 gttgacaatt attttgaata taatttatac taacttcggt ttattgaaac gatttagtta
     4441 agagagggga atgtttaata attattaaac ttgtaacaag attagctgat gtatctccgg
     4501 ctatattaat agttctaaat aggtatggaa aatcagctgg cttcttttta gcttctgctc
     4561 atcggtgcac tacaggtaac aaaagagaat ggctttcggt ccctcaaaat ttgcccgtta
     4621 taggttttta cagaatggaa gagaagaata cgctggctcc ctctactaca gtagaactct
     4681 ttccggagag ccgaagaagt ttccgctaag tttttagtag agaataaaag gaggtgataa
     4741 aaaaaataaa aacgtagaga attcggcagc aaaagcaaaa aacattatat tcagactgga
     4801 ggcgtatttg tgaaaagaaa ttttctaatc ttgtttgtat ctttgttagt attagtgata
     4861 ggttttactc agataggttt tgctcagtta tctgaagata ctcccagaga agagacacta
     4921 attactgatg tattaggtgg aagagcaagg aatcccggca aatttaacgc tttcgttgct
     4981 accggtataa cttcagatag gggcatacaa cagttaatgt tagaaccact ctggacaata
     5041 gaaccagcaa caggggaggt tattaactcc ctcgccgcag aaggtcctat ctacaatgaa
     5101 gacttcaccc agatgacagt aaagctgaga gagggttgct actggagtga tggagtcccg
     5161 ataactgcag atgatatagt atatggaata gaaatggcca tgaagcatcc tggaatggca
     5221 tatcatgacc agttaaatct ctatgttgac aaggtatata agactgatga ttatacagtg
     5281 gtgtttgagc tcaaagggcc aaatggtcgg tttcacacca actttttaga tagatgggga
     5341 gcatggagac cgtctccaaa acatattttc gaaaaagttg aagaccccct ggcttttgat
     5401 tttaatcccc ctataagtag tggcccttat gtacttaaag attatgacca ggcaggttac
     5461 tggactctct gggagagaag agaagactgg gataaaactc ctaccggaat gttgtatggt
     5521 atgcccaaac ctaaatatgt gcttttctac tactatggag atgcaaccaa caaagtaatt
     5581 gctcaagcaa accacaatct ggactcagtg gatttaaccc tggaggcatt ccgagcactt
     5641 ttagagcaaa ataaatactc acgaggttac aggaaagaat atccctgggt agtcaacatt
     5701 gacccctgca tgaccggatt aatgtttaac aacgatgtgt ccccctatga cttgaaagat
     5761 gtcagatggg ctttgactct agcaatagac atagtagatc acattggaat agcttttgat
     5821 ggagctgctg ccatgggagc gttacacatt cctcccatgc ctgtatatca aaaatggtat
     5881 tatgagccta tggaagagtg gttgaagaac ttcactttag atattgaagt gaatggagaa
     5941 cctttcaaac cttatgaccc cgatgccccc ttccgacttg ttgagtatgc aaagagtaga
     6001 ggctacactg tacctgaaga ccccgaagaa ataagagaag cctttggacc gggatggtgg
     6061 aaatatgctc ctgatgtagc agaacaatta ttgctcagga atggtttcac cagagatgta
     6121 gacggtaaat ggctcctgcc cgatggtgca ccctggaaaa tcaccatatt gaccagcact
     6181 aaccctgctc agccccaaca tagaaatgct cttgctgcgg cacagcagtg gagaaacttt
     6241 gggattgatg tgacagtaaa caccaatgaa gcggttaatg acctggtatt tcgaggagat
     6301 tttgaaattt caactaactg gcctgcaaat gaaccatggg gtggtcatcc tgacttatat
     6361 agaacggtca gcatcttcca ctctaagtat tacaaaccta taggtgaaat agccgttagt
     6421 tccaatgggg ctgcctcccg atggaaagac cccaggatgg atgaagtaat cgaaggaatg
     6481 gaaaaggttg attgggatag tgaagaaaac cttaggctgg gtatggaagg attaaaaata
     6541 cttgttgaag aaatgcccac cctacctact tttggttatc ctggcgtttt agcctgggat
     6601 gagtattact ggaccaattg gcctggtgca gaaaatccat atgttatacc ctatcaccac
     6661 tggccaaatc ttaaatacat gcttcccttc ctggaaccag caggaaataa ataaacacta
     6721 catcttatcc aggtctttaa gacctggata agatgtaaag ataaacatcg tttctcttcc
     6781 tcaagagctg gactcttaaa aaaactgaga cggagaatca aaaacatccc cttttctcct
     6841 gaaatgtaca gagagacact ttttttgcat gctcacccct ttacaggttt agcattatca
     6901 tatctatctg gtctggacac aaaacgacgg tttttgctgg atgcggcaat ccctttcagg
     6961 atcaatcgcg agcactggga gagacgcttt tccttcctca gtagtttttg tcttttgggg
     7021 attataaagg ggttctccca ctgaggaaag cgtttttctt tcctcagtta gtcttttata
     7081 tttttcagtt tttatctttt agggattata aggggacaag ccccattgag gaaggttctt
     7141 tcttcctcag ttgtttttgt ttttttgggt tttttgtttt ttttggggat tataaagggg
     7201 ttctcccctt tatagctcgg ggttatgagg gggcgccatc ccctcattgt ctggattccc
     7261 gatagaacca ttcaggaatg acaggcaagg gggtgatagg gggtttttcc cctcatagtt
     7321 ctgttttttc ctcctcagta gtttttgtct ttttattctg tttttgttta cgccgtaggc
     7381 tgtcttttgc ggttaaccat aacaatgtcc agagaaaaat tatggtacat gatgagcttt
     7441 acgggggagg aaatcttcta tgatataatt aaacggggtg ataaaattgg tctatcaaat
     7501 tatgatagta aaaatagggg ctcgttctca ccaggcactt aaagtgcagg agtgcttaac
     7561 caaatacgga tgcaacatta aaactcgttt agggcttcac gaggcctctc cagaatattg
     7621 tgctgaagac ggactgataa tcttagaatt gactgggaaa aaagaggaaa acgaacagct
     7681 gaagaaggag ctggaaagct tagaaggggt tactgttcaa tatctggaga tttagcattt
     7741 atttttgtta ctccaggaac cagtacttct actttaccag ggtttatttt agttcccctt
     7801 atggctatag ttttcttttc ttcattgata ctctgaataa ctcccagtcc taattcttct
     7861 cctctttcgg tataaagccc acagatagaa aaagagagag aagaaggatt aatcacccgg
     7921 catttattat cacctttttt ctctggagaa gtactgagaa tgaggaaaaa gttattagag
     7981 aattcaaaag taggtatacc ctctctcaaa tgatattcct ggagaaaaaa ataaggatga
     8041 taaggcgaga agaaacgaag attctcccac cccatttccc agttttctcc agaagaaaaa
     8101 tacaaagaga aaagccgttc cctgtgactg cgcctttttt cgtagtcttt tttagaggcc
     8161 cgggaagaag gagatacaac ccagaattgc tctccataaa attttttcag tacttcaaat
     8221 tcttcatccc ttccaatacc caggactaat tcgggcttaa tcagtttcat ctttaacatc
     8281 ttgagacgaa gaccagataa agaaaaaatt aaaccgcagg tatcaatcaa agtaatctgt
     8341 gttttagaag aagccatttt agttagttca gcagtgtata aagcggtagc ctctatcctt
     8401 ttttctggag ttacatctcc cacaaagcga aaataaggag taaataacct ttctcgaaga
     8461 gaaaggggaa attttttaga taccattacc atccctacag tggcaggagg accaacatta
     8521 gattgcccaa ggtctgaatc tacccatcct acttttttac cttgagaaac aaaaaaagaa
     8581 gaaagcaggc gaattaaagt agttttcccc cggtctggcg gggccaccac catcagtatt
     8641 ccccgaaaat tattcagagc atggggcaaa aaatcccatt ctggaggcca gaacatcttt
     8701 tcctcctaat atctaaggag ttgagagacg gtaacaaact tatagccttc tttttttaaa
     8761 ttctcaatta cctggggcag aactttaacc gttgtcgcta aattgtggaa aaggatgata
     8821 gccccatctc ttacttcttt ttttaccgtc tccagaattt cttcttcatc agtgagtaac
     8881 caatcttgag aatctaccca ccacaataag atgctgtagc cagtctgtcc gcaggcttct
     8941 tttacattac cgtttaccat tccccccggg ggacggaagt ataaagggat ttgaccggta
     9001 gctcgataaa tagcttcttc tcctcgttta atatccttta ccagagcttc tactgagtca
     9061 tcgctcgacc agcgatgaga gaaggaatgg ttacctattt catgtcctgc agctacaatc
     9121 tggcgagcaa cctcgggaga cttctctact tgggaaccga tcaggaaaaa agtagctggt
     9181 accccatgct tatctaaaat tttcaatact tctttagtga aagggctggg agcatcatca
     9241 aaagttagag ctacttcctt cctgacttga gagtttcccc ccagggagcg aaccaggttg
     9301 agaccgaaga cgttttcttc tgtttctata tctatacctt tcgcttcccg gggaagaaaa
     9361 attaagagta taaaacacga aacccagagc catactttga gtttgtgcat caggatttaa
     9421 taatactctg tagcttttcc tctattgttt ttttaggtac atatcctact attcttccca
     9481 cctctttacc tccctggaaa attaaaagag taggaatagc ctggatacca tatttggcag
     9541 caatttctgg agcctcatcg gtattcaatt tgccaacttt aacttttcct ttccaggaat
     9601 tggctaggtc ttctacaatg ggagccatca tccggcaagg catacaccag acagcccaga
     9661 aatcaaccag cactggaatt tctgaatcca gtacttcttc tttaaaatta tctttgttta
     9721 attctatcat gtttcttatg ctcctttctg acccatcttc aactctttac ccttaatcaa
     9781 tctctgaatg tttcctttgt gcctccagaa cacgagcgct gcagcaaata ttccccagat
     9841 tataaattcg gggggttttc caaacagata aagaaaaatg ggcattaaaa gagcaccact
     9901 tagagaacca agagaagaat aatgggtgat agatgccata attatccagg cgagaaagca
     9961 aagaattgcc gattcccagg aaagataaaa aagtattcct aaagttgtag ctactccttt
    10021 tcctcctcgg aagcggagaa atatggacca atcgtgaccc agaatcaaga gaaaaagagc
    10081 cagaaaccag agataaggat tagacaaaaa gtgagtggct aaataggcgc ccaaaaatcc
    10141 tttcagggcg tctcctactc cggcaatgag agcaggaaga aaaccagcta cccgggaaac
    10201 attggtagca ccaatactac cactcccata ttttcttata tccacacctt tcgctatcca
    10261 gcccactatc agtccgcaag ggaaagaacc cagaagataa ttaagtggta agacccacca
    10321 attaaccatc tatatcactc tccataacga gaaaagacta gactgccgtt atgacctccg
    10381 aatccaaaag aattggaaag agcgttcctg attttaagag agaaagcatg atcgggagtg
    10441 taatccagat cgcactccgg gtctttttct tccagattaa tggtgggggg aatgatgcca
    10501 tgataaatgg aaagagcagt aattgctccc tctagtgccc ctgctcctcc caaaagatgt
    10561 ccggtcatag atttggtaga gctaatttta atctggtaag ctctttctcc aaaaactttt
    10621 ttaatggcca gggtttctac cttatcattt aagggagtgg aagtaccatg agcattgatg
    10681 tagtcgatgt cttccggagt taagcaagca tcttctatcg ctaacttcat agaccgagct
    10741 gctccttctc cttcggggtc aggagcagtg atatggtaag catcacaggt agaaccatat
    10801 cctttcaact cagcgtaaat tcgtgctccc cgcttttttg ccctttcttc ctcttctaaa
    10861 attaatgctg ctgctccttc agccattacg aacccgtctc tagtgcgatc aaaagggcga
    10921 gaagctttag ccgggtcatc attatgagtg gaaagagctt tcatggcgca aaatccagcc
    10981 agagctaaag gagtgataga agcttctgcc cctactgcta ccatcaagtc tgcttctccc
    11041 ctctgaatga tgcgcagggc ttctcccaaa gcatgggtag aagcttcgca agcagtagaa
    11101 acacaactgt tgggtccttt cagcttcaat tgtatcgcca cattggctgc tcccatgtta
    11161 actatcatca tgggaatgaa gaaagcactg actttccccg gacctttagt cagaaggacc
    11221 tgatgctggt cctcaaaagt ttgaattcct ccaatacctg agcctaaaat aactcctgct
    11281 ctttctctat cgagattttc aagagacata gaagcatctt ccatagccat aagagaagcg
    11341 caaactgcaa attgggaaaa acggtccatc ctccggtatt ctttacgggg aaggaaatct
    11401 tcagggttga aatcctttac ttccccggct acctgactgt caaaatcacc agggtcaaag
    11461 cttttgattc tatcaatccc tgattttccc ccgatgattg attgccaaaa tttatcttta
    11521 ccactaccca ccgggcttat aactcctata cccgttacta ctacctttct ttccacctta
    11581 gaacctcctt taccatcgga taagggctga gccccaggtc aaaccggcac caaaaccaac
    11641 cagaagcact aaatcaccac cgttaatttt accctctcga tatgcttcat ctaaagcgag
    11701 aggaatggag gctgaagagg tattaccata tttgtgaaca tttacaaaaa ctctctcctc
    11761 aggaatgccc agtcttttag ctgccgaatt gatgattcga atgttagcct gatgagggat
    11821 aaagaggttc acctcatcca tttccacgtg gcctcttttt attacttcca gggaagcttc
    11881 ttccataatt ttcactgcga atttaaacac ttcattcccg ttcattttta tatagtgtaa
    11941 tcggttattg acggtatcca gagaagctgg cattcgagaa caaccagcgg ggagctccag
    12001 aagatgagcg cctcctccat cagaacccag ataggtagct atgattcccc cttctttgct
    12061 taatcccaga actgctgctc ctgccccatc gccgaataat acacaagtag agcggtcctg
    12121 ccagtctaat attttggaaa gagtctccgc tcccaccact aaagcttttt ctccccctcc
    12181 cccccggaga tatttttcag ccaaagataa agcataaaca aaccctgtac aacctgcttc
    12241 taaatcaaaa ctggctgcct ttcgggcttt taattctctc tgcaataagc aagcggtagc
    12301 gggaaagagc atatcaggag taaccgtagc cactactata aggtctaact cctcagcgga
    12361 aacctgggca gcttgaagcg ccctttgagc ggcgagtaaa gcaaaagtag aagtgctttc
    12421 ttccttctgt gctattcttc tttctttaat accggtacga gtagtaatcc attcatcgct
    12481 tgtatctact attttttcca gatcaaaatt agtaataact cgctctggta cgctagagcc
    12541 tgttcctaat atttttacct taccttcgct caatcttttg cctcctcttt tactaaagaa
    12601 aaacctatct ccccttccgc tactactgct tcattcaccc gcgctatccc ttttaacttg
    12661 ccaattcctc tcctcacctt gaggagagtt acttccatca ctaattggtc tccgggcata
    12721 actggacggc gaaatctaac cccatctaaa gaggtaaaat aaggaatgca acctcgaaac
    12781 tcctccatat tcatcatcat ggtggcacct acttgagcca tgctctctaa aatcaaaact
    12841 cccggcataa tgggttttcc tggaaaatgc ccctggaaga accactcatc catagtcaca
    12901 ttttttaatc ccaccacttt atcatcctcc agagatatta ttttatctac caagaggaaa
    12961 ggaaagcggt gaggtagtat ctcttggatt ttctttacat ctaattccat tcccacatcc
    13021 tcccattagc atatttaccc ctataatgta tctgaagtga atcaaattag caagaagtaa
    13081 ttttactatt atagatatac ctaactttga cagctataat ttaaaaatac tgatatatca
    13141 agggattagg tactcctttt ttgtttactc cactacagtt tcactgagaa taaacttggg
    13201 aa
//
LOCUS       DAOSVE010000045        12787 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_2887_length_12787_cov_69.086082, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000045 DAOSVE010000000
VERSION     DAOSVE010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 12787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12787
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2887_length_12787_cov_69.086082"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            326..1471
                     /locus_tag="PK016_05130"
     CDS             326..1471
                     /locus_tag="PK016_05130"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HXL02484.1"
                     /translation="MERTNQVWERAVPASEEEIISLVKQFDTPLFVINREKLLQNYRA
                     FQEFFPGIEIFYAVKANPHPFIIRTLKEVGSSFDVASFQEIELVLSQGVSPEKMIFAN
                     TIKRKKGLSYARQVGVNLMTYDNEEELKKIAMVYPDARLLVRIKTPSNGSRANLSYKF
                     GAEPDRAPELLLQAKKMGLKPVGISFHVGSPCSNVNNYLYSLQKVREIIEKVSGEISI
                     SIIDIGGGFPLSLYGEEEGGSLETFSEIINPYLQEFTEEGYRVIAEPGRVIVGSAAFL
                     ITKVIGKAARSGIMWYYLDDGIYGTFSAIPFDKAAFCFFPLRGSKGEQTAVLAGPTCD
                     SLDVVAENVLLPPLELDDLIVVPDIGAYSWASATNFNGFERAQVVMI"
     gene            1515..2240
                     /locus_tag="PK016_05135"
     CDS             1515..2240
                     /locus_tag="PK016_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL02485.1"
                     /translation="MLEIRGLGVRVGGEEVLQDVNLEIEPGEVHILLGPNGVGKTTLL
                     MTIMGMPGYEVTRGDILFQGESILPLSVEERAKMGIGMAFQKMPTIPGVKLRTLGEIM
                     VNGGSTFIEEVAEKLNCTYLLERSIGLGFSGGEAKRAELFQLFLQRPLLSLIDEPESG
                     VDLDNIALIGKALSELLEREQVRTKKRSGLIITHTGYILEYLNADMGHVLMEGRIVCR
                     GNPRDLFADIKEKGYQECLRCER"
     gene            2237..3484
                     /locus_tag="PK016_05140"
     CDS             2237..3484
                     /locus_tag="PK016_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HXL02486.1"
                     /translation="MKVKKDYLREIKERAEKALNKKAPLGPDVDLSEFYLCSPQEEVS
                     SVESISSSLQEAALYAGVELGEDKGSATYLQVDRSAVYQKIQDAFKGKLEIMAVSEAL
                     EKYPELLEYWWGLVPVDADKYTAFSEVCPMEGYFIRVFAHQKIEVPLQACLLLSEESR
                     IQSVHNLVILEEGAEANLITGCASVRGNKSGVHIGVSEFYLGDNSHLSFTMVHNWGEN
                     FHVRPRTVVKMGNNSFYSNTYVLLKPLSSLQTFPQAIMEGENADANFQSIIFAKGDSY
                     IDVGSSLLLKEKGCRGNSVSRVCAADNSQVYTRGRLISYHDEAQAHLECKGILFSRGA
                     EIISIPELEVHGAPQSRLSHEAAVGPIEEEAVNYLRSRGLSKEESLSLITRGFLNIDV
                     PGIPAVLKQYIDEVIRLTSRDIM"
     gene            3502..3735
                     /locus_tag="PK016_05145"
     CDS             3502..3735
                     /locus_tag="PK016_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HXL02487.1"
                     /translation="MSVQAYILVQVQTGRAQAIKKELAQLPQFLRVDRVMGPFDIIAL
                     VEVENNREIGELILKEIQSLNGVKRTLTCPVIP"
     gene            3741..4277
                     /locus_tag="PK016_05150"
     CDS             3741..4277
                     /locus_tag="PK016_05150"
                     /inference="COORDINATES: protein motif:HMM:NF013855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02488.1"
                     /translation="MRKKEKVSPRQEEILLFIQDYYKKNGYPPTVREIGEGVGLLSSC
                     TVHYHLEKLEEKGMIKRNRSKARTIEICSPDRENDLVVVPFLNGFSRNPISLPRSLLG
                     EGVFFFWRIEKNSWQNWHILKGDLLLVRKENEIKEESLVLTIKSDVVEIKRVNGSLSS
                     STDDGEIMGRIIGVWRKL"
     gene            4274..5416
                     /locus_tag="PK016_05155"
     CDS             4274..5416
                     /locus_tag="PK016_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HXL02489.1"
                     /translation="MKENFVFRLPPEIYFGGGEGKKAGELVKRWGEKALLVTGKKSIK
                     ETGILEEVLQSLEGAGVQCSIFDRVEPEPSLETVDNGLDLAYRERCDFVISLGGGSAQ
                     DCGKAIAGLCGNSHTVVPYFEGKILSSPGIPWIAVPTTAGTGSEMTTNAVLTDYRKKL
                     KKSFRSPYLLAKLVIIDPLYTRFMSPYLTAVSGIDALVQALEAYVSQGVNPVSEVLAE
                     EAIGVLWRYLPRAVKKGENLDYRKQVAKGSMISAMAFANSSSGAAHGLSHIVGPEFSI
                     VHGEACGIFFPRVVEFNREVLKDRYERIARLVGIEGEENLIDKFIEAFLSLMREIGLR
                     TSLGESGIKEEMLQGIVKEERIGKNLRENPRPMGKEEVYALLKASI"
     gene            5430..5771
                     /locus_tag="PK016_05160"
     CDS             5430..5771
                     /locus_tag="PK016_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXL02490.1"
                     /translation="MELVKEEDKTYRFGDWGIKYLFRGPRLDWGVFYLKPGTNLGAHY
                     HNQVEETFFVLEGRGFLRTEGQEIALEKGYALYLTPGEKHDLYAPEDSFLRGIFIKSP
                     YLPEDKIDCSI"
     gene            5886..6773
                     /locus_tag="PK016_05165"
     CDS             5886..6773
                     /locus_tag="PK016_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXL02491.1"
                     /translation="MVFAVNYHKEDVINHLWNRLADCDLCPRNCGVNRLRGERGFCGG
                     GVQVKIASFHPHGGEEFLLSGSQGSGTIFFSGCNMRCVYCQNYSISQGREGEELTEEE
                     LTQIMFFLQNQGCHNINLVTPTHFIPQIFSSLLKGKDCGLSVPVVYNTGGYDKKEVLE
                     KIEGLVDIYLPDMKYAREESACKYSEAQDYPRINREAVREMFRQVGEVEWEGEVAKRG
                     LIVRILIIPSLEEEAKENLRFLAENISRSVFVSLMKQYRPCYKADLFPELSRSVDERT
                     YQEIINYARGLGLRNLILQ"
     gene            6788..7315
                     /locus_tag="PK016_05170"
     CDS             6788..7315
                     /locus_tag="PK016_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HXL02492.1"
                     /translation="MEEALKQVANFMAVSARTAPKSGGKDFINVQILEGEEVKKLGEA
                     MVEYGEKQGIKGFIRDGKNVLDSSAVLLIGIKDAAVMNLNCGGCGYNSCSELQAAGKK
                     EVEFKGPQCAFRLLDLGIALGSAVKTASLFNVDNRIMYRIGAVARRAGISQDDVVMGI
                     PLSVKGKNIYFDRKE"
     gene            7326..8192
                     /locus_tag="PK016_05175"
     CDS             7326..8192
                     /locus_tag="PK016_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02493.1"
                     /translation="MEGALRIQDIIGLILAILIVAILISIILILRENARERRMWREED
                     YEDWGERKAKKSPSFSSKGEEGVSSILEDKIPEEIEKRRGWDTLPRQKGVEEIRKEEK
                     KESVVPPPPEEVFIPQEEPTIAKEPTRKGEPKQVVLERALRKIEELGLDINSLKPQIT
                     EEAIHFSPLLWEVREEVRVGRELGDMVIHTVFKNIPGYSRDEVVELYRSGDVSVVEKL
                     EARVKTGVGRDYLVATLDLDHFRIPELERLWRKRNLEDEEYELLLSLRFVDRNTKKDF
                     ARVVYAQITTED"
     gene            8401..10770
                     /locus_tag="PK016_05180"
     CDS             8401..10770
                     /locus_tag="PK016_05180"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HXL02494.1"
                     /translation="MKNWLNFVIALVALIISLVLVFQVSGWRTDLVNLEKRMGGVEEG
                     VRKAEDSLSQIQDKIQSLPIDLLDWGGRLKEMEASIAKTDESINSLTSAMEGLKGELA
                     SRDKAIQEIQNEMENRKKELEEIKSSFASLREELDNLNIDQRFSALLDQINEQGAEIA
                     ISLQALEETKKELEKINQLVLGIQVQSLSRAEWETVASQWEERFKKVEEEMSNLQNSL
                     VVIQEQQSQDLGKLKATIESEIRAIEERVNTLGQSLKEAQDSLREELDNLNIDQRFSA
                     LLDQINEQGAEIDGYLQELKKRVDTLETSFQDFRGVVEKLESTQSQQSQQLEDMRSLL
                     EKETASLEEKIVFLENQGREAEDTLRLLTQEKEELQGIIDTLHQSLSSVEEELGTISS
                     QWESKWQGVSEDISSWQEATLKDIEAKIASIKQELPDKRELEEWENKVNVLQEEGERL
                     KSELSSLEEEKNLALQEGKEKLSKLEEEILLWEKNLADYQKLMEQSIGEVNSRVSELS
                     AFLNSVEERLKEIETYSANWSKESQDTWESLKTSLSQLSQRLEEVSNFSQDILKKDLE
                     AKISSMEKKIEEMEKELNKEKQALEAGYPGTSVINESGEALGESVTLSSPGMGGEEIL
                     SVEEGSSESKEELSAEIKTLAQEKMELEEELSKKQEEMLKIDQELEELRKERGSWEEI
                     EKLEEERAEKEKAIIDLNQKLEEKNAQIETLQNKVAELTAQLEEKEKYTSYVILPWDN
                     LWDIARRYYRDGRKWPLIMEANKDVIKDPLSLKPYTEIKIPRCPDDEDC"
     gene            10849..11364
                     /locus_tag="PK016_05185"
     CDS             10849..11364
                     /locus_tag="PK016_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HXL02495.1"
                     /translation="MDFKELIQTRRSVRKFRAEEVEEEDLREILEAARLAPSAINLQP
                     WKFIVIKNDKKKRLIAQAAGGQEFVGEAPVVIVACATGRGGFIGGYMESWPVDVAISV
                     THLLLAAWDRGLGTCWVGDFDEEKIKEICEIPPEVRVVAIVPVGYPVKIPQVVPRRPW
                     EKIVSREVFSE"
     gene            11379..11603
                     /locus_tag="PK016_05190"
     CDS             11379..11603
                     /locus_tag="PK016_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HXL02496.1"
                     /translation="MVIRLEIGDVLTLKKAHPCGSKEWKIVRVGVDIGLRCVGCDRFV
                     MVSRRKLEGKIREIYREGQHLKPQESTIEA"
     gene            11607..12701
                     /gene="ychF"
                     /locus_tag="PK016_05195"
     CDS             11607..12701
                     /gene="ychF"
                     /locus_tag="PK016_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HXL02497.1"
                     /translation="MGLKAGIVGLPNSGKTTIFNLLARGKATVASYPFCTIDPNVGIV
                     EVPDGRLEILASLLHPPRVVRATIEFIDVAGLVEGASKGEGLGNQFLSNIRGVDAAIE
                     VIRFFQDEKIPHILGKVDPLQDKEIVDLELMLSDLEVIERRLEKLQDLTKKVKDERLK
                     LEKEILEKGKHWLEEGRPLRFLELNEEEKEVLKPYQFITLKPLIYVANLDENENSKHL
                     FDKVRKELEEKGEKIIPVFARLEEELEEIPVEEREVFIQEFGIEDTGLNLVVKEAYQL
                     LGLITFYTFGDKELRAWELPRGSKAPQAAGRIHTDMEKGFIKAEVINYEELVAIGSWE
                     RAREEGRIRQEGRDYEIKDGDLVYFRFSPR"
BASE COUNT         4052 a         1910 c         3219 g         3606 t
ORIGIN      
        1 tagagtataa ggtcttcctc aacagtgagg acttgtaagc cgtgtaaaat aaggatatcc
       61 tccatcgcct ttattaagga atccgcctct tgctcattga tatctaaaaa cctctttctg
      121 tctttaatct tcataatttt gtgttttaag tatttaaaga agagagctat agaggttttc
      181 tcctatagct gtgacattat cacagaataa ctacaaaaat aaaatttaat tattgcaaaa
      241 actgctttaa gttattatac tgcacacgga cgcaaaaaag aaattgtgtt taacacataa
      301 aactcatagt gggtgatgcc tattcatgga aagaactaat caagtttggg agagagctgt
      361 accggctagc gaagaagaga ttatttctct ggtgaagcaa tttgatactc ctctttttgt
      421 aattaacagg gaaaaattat tacagaatta tcgtgctttt caagaatttt tcccaggaat
      481 agagattttc tatgcggtaa aagctaatcc tcatcctttt attatcagaa ctttgaaaga
      541 agtagggtct tcttttgacg tggcttcctt tcaggaaata gaactggttt taagtcaggg
      601 agtttccccg gagaaaatga tttttgccaa taccatcaaa agaaaaaaag gcctaagcta
      661 tgcccggcag gtaggagtaa accttatgac ttacgataac gaggaagagc tgaaaaaaat
      721 tgctatggtt tatcctgacg cccggttgtt agtaaggata aaaactcctt ctaatgggtc
      781 ccgagccaat ctttcctaca aatttggtgc cgagcctgat agggctcctg aactactcct
      841 tcaagcaaaa aagatgggac ttaaaccagt aggtataagt tttcatgtag gttctccttg
      901 ttcaaatgtt aataattatc tttattctct gcaaaaagtt agagaaatta tagaaaaggt
      961 ttcaggagaa atctctatct ctattataga tataggagga ggttttcctc ttagcctcta
     1021 tggggaggaa gaaggaggga gtctggagac gtttagtgaa ataatcaatc cctatcttca
     1081 agaatttaca gaagaaggtt accgagttat agctgagccg ggaagagtaa tagtgggcag
     1141 tgcggctttt ctaataacta aagtgattgg aaaagccgca cgctccggga ttatgtggta
     1201 ttatctggat gatggaatat acggaacttt ttctgccatt cctttcgata aggccgcttt
     1261 ttgtttcttt cctttgcgag gctcaaaggg agagcagaca gctgttctgg ccggtcctac
     1321 ttgtgattct ttagatgtgg tggcagagaa tgttctacta ccacctttag agctggacga
     1381 cctgattgta gttcctgata ttggagctta ttcctgggct tcagctacca attttaatgg
     1441 ttttgaaaga gcacaagtag ttatgattta gtttggagcg aataaagtgt tataattgtt
     1501 ctgaaggtga tggtatgtta gaaattagag gattgggagt aagagtagga ggagaagaag
     1561 tcctccagga tgtaaatctg gagatcgaac cgggtgaagt tcatattttg cttggtccta
     1621 atggagtggg taaaactact cttttaatga ctattatggg aatgcccggg tatgaagtaa
     1681 cgaggggaga tatacttttc caaggggaaa gtatccttcc tctcagtgta gaagagagag
     1741 ctaagatggg aataggcatg gcttttcaaa agatgcccac cattcctggg gttaagctta
     1801 gaaccttggg ggaaataatg gttaatggag gttctacttt tatagaagaa gtagcagaga
     1861 aacttaactg tacttacctc ctggagcgta gtatcggttt aggtttttca ggaggagaag
     1921 ctaaaagggc agagctcttt cagctttttt tgcagcgtcc tcttctttct ctcatagatg
     1981 aaccagaatc tggagtagac ctcgacaaca ttgctttaat tggaaaagcc ttaagtgagc
     2041 ttttagagag agagcaagta aggacaaaaa aaaggtctgg tcttatcatt acccacaccg
     2101 gatatatttt agaatatttg aatgctgata tgggacatgt tcttatggaa ggacgaattg
     2161 tgtgccgggg taacccccga gacctttttg ctgatattaa agagaaaggt tatcaggaat
     2221 gtctgaggtg tgaacgatga aagtgaaaaa agattatttg agagaaataa aagagagagc
     2281 tgaaaaagct ttaaataaaa aagcaccttt aggtcctgat gtggatttga gtgaatttta
     2341 tctctgttct cctcaagaag aggtgagcag tgtggaaagt atttcttctt ccctgcaaga
     2401 agcagctctt tatgctggag tagagctggg ggaggataaa ggttcagcca cttatcttca
     2461 ggtagaccgt tcggcagttt accagaaaat acaggacgct tttaaaggaa aactggagat
     2521 tatggcagta agtgaggctc tggagaaata tcctgagctt ctggaatact ggtggggatt
     2581 ggtacctgta gatgctgata aatatactgc tttttctgaa gtttgcccca tggaagggta
     2641 ttttattcga gtctttgctc atcaaaaaat agaagttcct ctccaggcct gtcttctcct
     2701 ttctgaagag tcccggatac agagtgtcca taatctggta atattagaag aaggagcaga
     2761 agctaatttg attactggtt gtgcttcagt tcggggcaac aagagcggtg tacatattgg
     2821 agtcagtgag ttttatttag gagacaactc acatctttcc tttaccatgg ttcataactg
     2881 gggagaaaat tttcatgttc gtcctcgaac agtggtaaag atgggcaata attctttcta
     2941 cagtaatact tatgtcttgc ttaagcctct ctcttctttg caaacttttc ctcaggctat
     3001 catggaggga gaaaatgccg atgctaactt tcaaagtatt atttttgcta agggagattc
     3061 ctatatcgat gttggttcct ctttgctttt gaaggagaag gggtgtcgag gtaactcggt
     3121 ttctcgggta tgtgctgcgg ataactctca agtttacacc cgaggaagac tgatttctta
     3181 ccatgacgaa gcacaagccc atttagagtg taaagggata ctattttcca gaggagcgga
     3241 aatcatttct ataccggaac tggaagtaca tggagctcct caaagtcggc tgtctcatga
     3301 ggcggcagta ggtcctattg aggaagaggc agtgaattat ctgcgctctc gaggactttc
     3361 taaagaagag tctctgtcgc ttattacccg ggggtttttg aatatcgatg ttcctgggat
     3421 tcctgctgtg ttgaagcaat atattgatga agtaattcgt ctcacctctc gggatattat
     3481 gtagtagagg gggttctggt tttgtcggtt caggcttata ttctggttca ggtgcaaact
     3541 ggtagggcgc aggccattaa aaaggagttg gctcaattgc ctcagttttt gcgggtggat
     3601 agggttatgg ggccttttga catcattgct ttggtagagg tagaaaataa ccgggaaatt
     3661 ggtgaattga ttctcaaaga aattcaatcc ctgaacgggg taaaaagaac ccttacctgc
     3721 ccggtaatac cctgatagct atgagaaaaa aagagaaggt ttctccccgt caggaagaaa
     3781 tcttgctttt tattcaggat tactataaaa aaaatggtta tcctcctacg gttagagaaa
     3841 ttggagaggg agtaggttta ttgtcttctt gcaccgttca ttaccacctc gagaagctag
     3901 aagagaaagg aatgataaaa agaaatcgtt ctaaagcccg aactatagaa atatgttccc
     3961 ctgacaggga aaacgattta gttgttgttc cctttttaaa tggtttttct cgaaatccta
     4021 tttctttacc ccgcagtctt ttaggagaag gagttttttt cttctggcga atagaaaaaa
     4081 atagctggca aaactggcat attctaaaag gagatttgct tttagttaga aaagaaaacg
     4141 agataaaaga agaaagcctg gttttgacta tcaaaagtga cgtagtagag attaaaagag
     4201 ttaatggtag tttatcttct tcaacggatg atggggagat tatgggaaga ataatagggg
     4261 tatggaggaa actatgaaag aaaattttgt ctttcgccta ccccctgaaa tttattttgg
     4321 tggaggagaa ggtaaaaaag ctggagagtt agtaaaaagg tggggagaaa aggcacttct
     4381 tgttaccggg aaaaaatcga taaaggaaac cgggatattg gaagaggttc ttcaatctct
     4441 tgaaggagct ggagtgcaat gttctatctt tgaccgggta gaacctgagc cttcattaga
     4501 aacggtagat aatggtctgg atttagctta tcgagaacga tgtgattttg tcatttcttt
     4561 aggtggggga agtgctcaag attgcggtaa agctattgct ggattgtgtg gtaactctca
     4621 tactgtggta ccttattttg aggggaaaat tctttcctct ccgggaattc cctggatagc
     4681 tgttcctact actgccggga caggatcaga aatgactact aatgcggttc ttactgatta
     4741 tcgcaagaaa ctaaaaaaga gtttccgcag cccttatctt ttagctaaac tggtaatcat
     4801 tgacccgctg tatactcgat ttatgagccc ttatttaact gcagtaagtg gcatagatgc
     4861 actggttcag gctttagaag cttatgtgag ccagggggta aacccggtga gcgaggttct
     4921 ggctgaggaa gcaataggag tgctgtggag atatcttcct cgggcagtta aaaaaggaga
     4981 aaacttagat taccgaaagc aggtggcaaa aggaagcatg ataagtgcca tggcttttgc
     5041 taactcttct tctggtgcgg ctcatggact gtctcatatt gttggtccag agttttctat
     5101 agttcatggg gaagcttgcg gaattttctt tccccgggtg gtagagttta atcgggaagt
     5161 gctaaaagat agatatgaaa gaatagctcg cctggtaggt atagaagggg aagaaaattt
     5221 aattgataaa tttatagaag cctttttaag tctaatgaga gaaattggct tgcgaaccag
     5281 tttgggagag tcgggaatca aagaagaaat gcttcagggg atagtgaagg aagaaaggat
     5341 aggaaaaaat cttcgagaaa acccccgtcc tatggggaag gaagaagttt atgctctttt
     5401 aaaagcttct atttaaggag gaagaactta tggagttagt aaaagaggaa gataaaactt
     5461 atcgctttgg agattgggga ataaaatatc ttttccgagg tcctcgcttg gattggggag
     5521 tattttatct taagccaggg actaatctgg gagctcacta tcataatcaa gtagaggaaa
     5581 cttttttcgt cctggaaggt agaggatttc tgagaactga aggccaggag atcgccttag
     5641 agaaaggata tgccttgtat cttaccccgg gagaaaaaca tgacctttat gcaccggaag
     5701 attccttttt gcgaggtatt tttataaaaa gtccttattt gcctgaggac aagatagatt
     5761 gttctatata acagtaagat atttctttat aattcccgaa gaaaaaaata aatgagagag
     5821 ggaagatatc ttcttcgata gaggagaagc gtctacttgt agtttctgga gggtgtaata
     5881 attaaatggt ttttgcggtc aactatcata aagaggatgt aattaatcat ctctggaata
     5941 ggttagcaga ttgtgacctg tgtccccgaa attgtggggt taatcgtttg aggggggaga
     6001 gaggtttttg tgggggagga gttcaggtga aaatagccag ttttcatccc catggaggag
     6061 aagaatttct cttgagtggt agccagggtt caggtactat tttcttttct ggttgtaata
     6121 tgaggtgtgt ttattgccag aattattcta taagtcaggg aagagaagga gaggagctta
     6181 ccgaagagga gttaacgcag attatgtttt tcttgcagaa tcaaggttgt cataatataa
     6241 acctggttac ccctactcat tttattcctc agatttttag ttctctcctt aaagggaaag
     6301 attgtggtct atctgtcccg gtggtttata atactggtgg gtatgataaa aaagaagttt
     6361 tggagaaaat tgagggatta gtagatatat atttaccaga tatgaagtat gcccgggagg
     6421 aaagtgcctg taaatactct gaagctcagg attatccccg gattaacagg gaagcagtga
     6481 gagagatgtt ccgccaggta ggggaagtag agtgggaagg agaagtggca aagcgaggac
     6541 tgatagtgag aattttaatc attccttctt tagaagagga agctaaggaa aacttgcgat
     6601 ttttggctga gaatatttct cgaagtgttt ttgtttctct tatgaaacag tatcgtcctt
     6661 gttataaggc tgatttattt ccggaattga gtcggagtgt ggatgagaga acttaccagg
     6721 agataataaa ttatgcacgg ggtctggggc taagaaactt aattctacaa taaaggaggt
     6781 ataaaagttg gaagaagctt taaaacaggt agctaatttt atggctgttt ctgctcgcac
     6841 tgcgcctaaa tcaggaggta aagattttat aaatgttcaa atattggaag gagaagaggt
     6901 aaaaaaatta ggtgaggcaa tggttgaata tggagaaaag cagggaataa aaggatttat
     6961 aagagatggg aaaaacgttt tggattcttc agcagttttg ttgataggaa taaaggatgc
     7021 ggcggtaatg aaccttaact gtggtgggtg tggctataat agctgttctg aacttcaggc
     7081 agcaggaaag aaagaggttg agtttaaagg acctcaatgt gcctttcgtc ttttagattt
     7141 aggaattgct ctgggttcgg cagtcaaaac tgcttctctt tttaatgtgg ataatcgtat
     7201 tatgtatcga attggagcgg tggcaaggag ggcagggatt tcccaagatg atgtagtgat
     7261 gggcattccc ctttcagtaa aagggaaaaa tatttatttc gaccggaaag aatagaggtg
     7321 taactatgga gggagcttta agaattcagg atatcatagg tttgatttta gccattttga
     7381 ttgtggctat tttgataagc atcattctta ttctccggga aaatgctcgg gagaggagaa
     7441 tgtggcggga agaagattat gaagattggg gagagaggaa agctaaaaag agcccttctt
     7501 tttcttctaa aggagaagaa ggagtttctt ctattctgga ggataaaatt cctgaagaga
     7561 tagaaaagag gagaggatgg gacactcttc ctcgacagaa aggtgttgaa gaaataagga
     7621 aagaggagaa aaaagaaagc gttgttcctc cgcctcccga agaagttttt attcctcagg
     7681 aagagccaac tatagctaaa gagccaacca gaaaagggga accgaaacag gtggttctgg
     7741 agcgagcatt aaggaaaata gaagaactgg gattagacat caactctcta aaaccgcaaa
     7801 ttacggaaga ggcgatacat ttttctcctt tgttgtggga agtgcgggaa gaagtaaggg
     7861 tgggaagaga attaggtgat atggttattc ataccgtttt taaaaatatt cccggttatt
     7921 ctcgagacga agtagtggaa ctatatcgtt ccggtgatgt atcggtagta gaaaaattag
     7981 aagctcgggt taagactggc gtagggcgag attacctggt ggctacttta gatttagacc
     8041 atttccgtat tcctgagtta gaacgattat ggagaaaaag aaatttagaa gatgaggaat
     8101 atgaacttct actttctttg aggtttgtag atcgcaatac taaaaaagac tttgcccggg
     8161 tggtttacgc tcagattacc actgaagatt aatgcttatg aggtctcttt gtatcatcta
     8221 acctttgttg atttttatct ttttccctga acattccagg gctaaaggta aaatactacc
     8281 atatattggt gaataatcga aaattatgtt tcttcagtct tcttaatatg ttattgttaa
     8341 taggatgaat gttaagatct ggggcaaaaa aatgctataa tttttcaggg ggtgataaca
     8401 gtgaagaact ggctaaactt tgtaattgct ttagtggctt taattatctc tttagttttg
     8461 gtttttcagg tttcaggatg gaggacagat ttagttaatc tggaaaaaag aatgggggga
     8521 gtagaagaag gggtaaggaa agcagaagat tctctatctc aaattcaaga taaaattcaa
     8581 tctcttccta ttgatttgct ggattgggga ggaagattaa aggagatgga agcctctatt
     8641 gctaaaactg atgagagtat aaatagttta acctctgcga tggagggatt gaaaggagag
     8701 cttgcttccc gggataaagc tatccaggaa atacagaatg aaatggagaa tcggaaaaaa
     8761 gagctggaag aaattaaaag tagtttcgct tctctcagag aggagcttga taatctgaac
     8821 attgaccagc gtttctctgc tcttttagac cagattaatg agcaaggtgc agaaattgct
     8881 atttctcttc aagcactgga ggagacgaaa aaagaactgg aaaaaataaa tcaactggta
     8941 ttaggaattc aagtccaatc cctaagccgg gcagagtggg aaactgtagc cagtcaatgg
     9001 gaagagagat ttaaaaaagt agaagaagaa atgagtaatc tgcaaaattc tctggtagtt
     9061 attcaagaac aacaatctca agatttaggg aagttgaagg caactattga atctgaaata
     9121 agagctatag aggaaagagt taacactctg gggcagagtt tgaaagaggc tcaagattct
     9181 ctcagagagg agcttgataa tctgaacatt gaccagcgtt tctctgctct tttagaccag
     9241 attaatgagc aaggtgcaga aattgatggc tatctccagg aactgaaaaa aagagttgat
     9301 actctggaaa cttcgtttca agactttcga ggagtagtag aaaagttaga atcaacccag
     9361 agtcaacaat ctcaacagtt agaagatatg cggtctcttt tagaaaaaga gaccgcatcg
     9421 ttggaagaga aaatagtttt tctggaaaat caggggagag aggcggagga cactcttcgc
     9481 ctcttgactc aagaaaaaga agagcttcaa gggattatcg ataccttaca ccagagtcta
     9541 tcatcggtgg aagaggaatt gggtactatt tcttctcagt gggagagcaa atggcaaggg
     9601 gtgagtgagg atataagtag ctggcaagaa gcaactctta aagacattga ggctaagata
     9661 gcaagtatta agcaagagtt accggataaa agagagctgg aggagtggga aaacaaagtt
     9721 aatgttctac aagaagaggg agaaagatta aaaagtgagc tttccagtct cgaggaagaa
     9781 aaaaatctcg ctttgcaaga agggaaagag aaactgagta aactggagga ggaaattctt
     9841 ttgtgggaaa agaacctggc ggattaccag aaactaatgg agcaatccat cggggaagtc
     9901 aatagccggg tgagtgaatt aagtgccttt ctaaattcag tggaagaacg cctgaaggaa
     9961 attgaaactt atagtgctaa ctggtctaaa gagagccagg atacctggga aagcttgaaa
    10021 acttctttat ctcagctctc ccagagatta gaagaggtga gcaatttcag ccaagatatt
    10081 ttaaagaagg acctggaggc taagattagt tcaatggaaa agaaaataga agaaatggaa
    10141 aaagaactga ataaagagaa acaagccctg gaagctggct atcctggaac ttctgttata
    10201 aatgaaagtg gagaagcctt gggggaaagc gttactcttt cttcccccgg aatgggagga
    10261 gaagaaatat tatcggtaga ggaaggatcg tcggagagta aagaagaatt gtctgcagag
    10321 ataaaaactt tagcccagga aaaaatggag ttagaagaag agttaagtaa aaagcaggaa
    10381 gaaatgctta aaatagacca ggagttagaa gaattgagaa aagaaagagg atcctgggaa
    10441 gagattgaaa aattagaaga agaaagagca gaaaaggaaa aagccataat agacttaaac
    10501 cagaaattag aagagaaaaa tgctcaaata gaaactctgc aaaataaagt agctgagcta
    10561 actgctcaac tggaagaaaa agagaaatac acttcttatg tgattcttcc ctgggataat
    10621 ttatgggata ttgcccgtcg ttattatcga gatgggcgaa aatggccctt gattatggaa
    10681 gctaataagg atgttatcaa agaccctctt agcttaaaac cttatacgga aataaaaatc
    10741 cctcgctgtc ctgatgatga ggattgttaa tcaaactgat acaggccagg atttgtggtt
    10801 ttctaattta ctcttattta ttaccagata agagaggagt agataggtat ggattttaaa
    10861 gaactgatac agactcgaag aagtgttcga aaatttcgag cggaagaagt agaagaggaa
    10921 gatttgagag aaatattgga ggcagcacgt ttagctccct cagctataaa tctccagccc
    10981 tggaaattta tagtgataaa gaatgataag aagaaaaggc tgattgctca ggcagcagga
    11041 ggtcaggagt ttgtgggaga agctcctgtg gttattgttg cctgtgctac agggagagga
    11101 ggttttatcg gcgggtatat ggaaagctgg ccggtggacg tagctatttc tgttactcat
    11161 cttttactgg cagcctggga tagaggttta ggtacttgct gggtggggga ttttgatgag
    11221 gaaaaaatta aagaaatttg cgagattcct cctgaagtaa gagtagtagc gatagttcct
    11281 gttggatatc cggtgaagat tcctcaggtc gttcctcgcc ggccctggga aaaaatagta
    11341 agcagagagg tttttagtga gtaaggagga tagttttagt ggtaatcagg ctggaaattg
    11401 gcgatgtttt gaccttgaaa aaagcccatc cttgcggaag caaggagtgg aagatagtca
    11461 gagtaggagt ggatattggc ttgagatgtg tgggctgtga tagatttgtg atggtttccc
    11521 gacgaaaact ggaaggaaaa attcgggaaa tttatcggga aggacagcac ttgaaacctc
    11581 aagaaagtac cattgaggca taaaatatgg gtcttaaagc aggcatagta ggtttaccta
    11641 attcaggaaa aactactatt tttaatcttt tagccagagg taaggcgacg gtggctagct
    11701 accccttctg tactattgac cctaatgtgg ggatagtgga ggttcctgat ggacggctgg
    11761 aaatcttagc ttctcttttg catcctcctc gagtggtgag agccaccatc gagtttatag
    11821 atgtagctgg tttagtagaa ggagcgagca aaggagaagg cctgggcaac caatttttga
    11881 gtaacatcag gggggtagat gcagcaatag aggtaatcag gttttttcaa gatgaaaaga
    11941 taccccatat tctgggaaaa gtcgacccct tacaggataa agaaatagtg gacctggagt
    12001 tgatgctttc ggatttagaa gttatagagc gtcgcctgga aaaattacag gatttaacta
    12061 aaaaagttaa agatgaacga ttaaagctgg agaaagaaat tttagaaaaa ggaaaacatt
    12121 ggttagaaga aggtaggccg cttcgttttc tggaactcaa tgaagaagaa aaagaagtat
    12181 taaaacctta ccagtttata actttaaagc ctcttatcta tgtggccaat ctagatgaaa
    12241 atgaaaatag caaacatctt tttgataagg ttagaaagga actggaagaa aaaggagaga
    12301 agataatacc agtgtttgcc aggctggaag aagaattaga ggaaattcct gttgaagaaa
    12361 gagaagtatt tattcaggag tttggtattg aagatacggg attaaatctg gtggttaaag
    12421 aagcttacca actcttaggt ttaattactt tttatacttt tggagataaa gaattgcgag
    12481 cctgggaact tccccgagga agcaaagctc ctcaggctgc aggaaggata catactgata
    12541 tggagaaagg atttattaag gctgaagtta taaactatga agagttagta gctattggtt
    12601 cttgggaaag agctcgggag gagggaagaa ttcgccagga aggcagagac tatgaaataa
    12661 aagatggaga tttagtttac tttcgttttt ctccccgttg agagttattc tgatatcgac
    12721 cccataactt tcaaattatt ttccagagat gtaaaaacaa cttaattctg taatcatctt
    12781 tttggct
//
LOCUS       DAOSVE010000046        12415 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_2964_length_12415_cov_69.673786, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000046 DAOSVE010000000
VERSION     DAOSVE010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 12415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12415
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2964_length_12415_cov_69.673786"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            586..1026
                     /gene="rplM"
                     /locus_tag="PK016_05200"
     CDS             586..1026
                     /gene="rplM"
                     /locus_tag="PK016_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HXL02498.1"
                     /translation="MSTKTYVPDVEKIEKKWYVVDAEGQALGRLASQIAQILMGKHKS
                     IYTPFMDVGDFVIVVNAEKVKVTGKKREQKLYRWHTGYPGGFKSETLGSLLNRRPEEV
                     IRRAVWGMVPHNRLGRKMIRKLKVYSGPSHPHQAQNPIPLKDKE"
     gene            1032..1427
                     /gene="rpsI"
                     /locus_tag="PK016_05205"
     CDS             1032..1427
                     /gene="rpsI"
                     /locus_tag="PK016_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HXL02499.1"
                     /translation="MAVNPKYYATGRRKTSVAKVWLTLGSGKIVVNGRDFRDYFPLPA
                     WQILLQKPLALTGSEARFDVEAEVVGGGLSGQAGALAHGIARSLALIDENLRPTLKKA
                     GLLTRDPRMKERKKYGQRGARARFQFSKR"
     gene            1523..2602
                     /locus_tag="PK016_05210"
     CDS             1523..2602
                     /locus_tag="PK016_05210"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HXL02500.1"
                     /translation="MAVGAYPLLEIDLSIIERNVGIILEKCASWHLHPVVVTKGFLAD
                     SPLVYLFYRSGIRSFADSNLDNLLRSKKLLPSGVRLLLIRLPMKEEVEEIVAQEIIPF
                     VSHWEMVKALNEEAEKKGKVLPLFLALESGDAREGFLPEEIEEMGDKIGNFSHIKVEG
                     ICSTLACLSGILPDEEILKRLVAFKRGWEEKWGRKIILSVGGTTFLSLWEKEKEIRGV
                     DEIRLGEALLFGNDISRGRSIEWLEGGAFTLKAEIVEIKSKEPDRGKKTGFDAFGEPN
                     FSLERGKRKRMILALGKQDIDEHQLYFSDENAKIIGATSNYLVMDIEESDRVYQLGEV
                     VQFRAGYGAVLRAFLSPYVRKVYHW"
     gene            2613..3554
                     /gene="argF"
                     /locus_tag="PK016_05215"
     CDS             2613..3554
                     /gene="argF"
                     /locus_tag="PK016_05215"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HXL02501.1"
                     /translation="MDKKRFRGRDFLDLADYSREELHYLLDTARELKRMDMMGVRESK
                     PLKGKIVALLFEKPSLRTRVSFEVAVRHLGGESFYLGPQEVGLGQREAVKDVAQVLDR
                     MVDGLVVRTFAHENLLEIAQFASIPVINGLSDLYHPCQVLGDLLTLEEKKGSLRGIKT
                     CFLGDGNNVCHSWINAASILGLELTVSTPEHYRPSSRIWKDAQERGKESGAQIIYEVD
                     PQKAIEGAEVVYTDVWVSMGKEAEKEERKVFLQPYQVNSHLLSKASPDCIVMHCMPAK
                     RGEEITDEVMDGPHSVVVDEAENRLHAQKALLALILS"
     gene            3567..4328
                     /gene="cdaA"
                     /locus_tag="PK016_05220"
     CDS             3567..4328
                     /gene="cdaA"
                     /locus_tag="PK016_05220"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HXL02502.1"
                     /translation="MAPAWLWRVVPEIIILYYLFYRLGSLLRGTSLFLSFKWLPLFFL
                     LAGTSNFLGFRELKFFWELLIFTYLGGSLIVFQPELRRIFFNRIHHRGEPVRDYFFQN
                     EESRSELINNITLACQTLSRKKIGALIVLERNNNLRDFIQTGVAIDAFFSPELLFSLF
                     LPESPLHDGAAILRENRIVAAGCILPLTESTEVKKLVGTRHRAGIGITEQTDALALVI
                     SEETGKISLAVHGKMAWDIEIGALKKMLKILYKRV"
     gene            4332..4466
                     /locus_tag="PK016_05225"
     CDS             4332..4466
                     /locus_tag="PK016_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02503.1"
                     /translation="MNVVEILKKRWQNFERRWKRDKEIRIWACLLALSFWFFLWSERL
                     "
     gene            4513..6597
                     /gene="fusA"
                     /locus_tag="PK016_05230"
     CDS             4513..6597
                     /gene="fusA"
                     /locus_tag="PK016_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HXL02504.1"
                     /translation="MNNFASKNIRNVGLFSHAGSGKTTLSEALLFNAGLLSRRGRVEE
                     GNTVSDWQPEEIKRGISIDLSILPWEWKGCKVNLIDTPGYADFVGNVLGAVRAVDSGV
                     VLLCATSGVEVGTEKVWQYLSQENLPVIFFVNRMDREGANFDEAVEELRKKISPRVTP
                     LFIPVGKESNFTGVVDLLHFRALYFGKEGKVEEGEIPEEMKEAVQNARENLVENIAET
                     SDELLDLYLEGKEISPSQLEETLRIAIRERKIFPVLCGSGGENKGVDVLADFLVDFAP
                     SPLERPPVRGINPLTKEEEERKCDENEPFFALVFKIISDPYVGKLALFRVFSGKISSD
                     SRLYNASRGVEEKIGQLLFLRGKNQEPVKEVGAGDIGVLAKIGEVYIGDTLADREHPI
                     VFPPISYPEPTYMAAIRPLGKGDEEKISQALSRLMEEDPTFRLYRDPDTKEDIVYGMG
                     DIHLDVLVEKMKRKFGVEVSLTIPQVPYKETIKKSTKAEGKYKRQSGGRGQYGHAFIE
                     LEPLPRGQGFEFVDKIVGGVIPRNYIPAVEKGVQEAMQEGILAGYPVIDVRVTVFDGS
                     YHPVDSSDMAFKIAGSMAFKKGAGEAEPVLLEPIMNVEVIVPEEYMGDVIGDLNSRRG
                     RILGMDPEDGYQKIKALVPMAELFRYSVDLRSITQGRGYFTMKFSHYEEVPAQIAETI
                     IEKAKKEKEAVS"
     gene            6594..6986
                     /gene="acpS"
                     /locus_tag="PK016_05235"
     CDS             6594..6986
                     /gene="acpS"
                     /locus_tag="PK016_05235"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HXL02505.1"
                     /translation="MRQRILVGVDIVEIERVEKAVERFGDRFLNKLFQEEELRFYSSP
                     KRQREGIAALFCGKEAVKKLFLSKNFLLPWREVKIEHTSRGKPEVRLSSRVPAFFEEI
                     DISISHSRQFVVAVAIGVEKEGESVGIG"
     gene            6973..8547
                     /locus_tag="PK016_05240"
     CDS             6973..8547
                     /locus_tag="PK016_05240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HXL02506.1"
                     /translation="MELVSSETMQKLEKTLSEKYGVDTLLLMENAGRGVAEEIIKIIQ
                     SHSFSKVYILCGMGNNGGDGLAIARRLRLRLPSLSLLIYLLGKGENLKKDARFNYQVV
                     KNLSLPLVEVSSSQEVELERGALVVDALLGTGITREVEGIYREVIEKINASGNFVVAV
                     DVPSGVEASDGRIMGEAVRANLTVTLGLIKRGLCSFPGREYVGKLKLVDIGIPLPLEE
                     AGFKADGFLITPSMVKEMLPQRHWDTHKISAGVLGVIAGSSSLLGAGLLVCSGGYRSG
                     AGMVVWPLDFSLSPLVKLFLPEVVSVNLSSSGEWHYSLSHWEVVEKAFQDRKCGALAV
                     GPGMGTNPSTSLFLEKVLENFRGVLDADALNIISQNRDNWKGRLASWVITPHSGEMAR
                     ILGVSVPEVNENRIAASLEASSLLGCVTVLKGAGTVIASPQGEVFLNPTGTPVLASAG
                     SGDVLTGIIAGLMAQGMSPLNSAISGVFLHGLAGELLEREGKKQILSREIAEKLNEAR
                     RMVENGEYTLSFLDGD"
     gene            8510..9646
                     /gene="alr"
                     /locus_tag="PK016_05245"
     CDS             8510..9646
                     /gene="alr"
                     /locus_tag="PK016_05245"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HXL02507.1"
                     /translation="MENILFPFWMEIDAQNLHHNVEELKRWSSSSLMAVVKSEAYGHG
                     WEAVNLLYDYGLRKFGVASVEEAHELRKRGLRGDIYLLGGFFTEEAETILEEELTPVV
                     SSDKELQALEEVAKKSGKVIPFHLKVDTGMGRMGFLPQEINDRWWKKWGNNCHLSLQG
                     IMTHFASAESDPVYTRFQFDNFLSFLHRLPENSPSLERHCCNSAAFLSFPEMHLDLSR
                     VGIAFWGVSPWGRKSALSLDLKPVMRIKARVKQVKTLPPGNRVGYGGTFTTYRETKLA
                     AISIGYSQGLFRSLSNRMEVLIKGKRAPSRGNISMDQMTVEVTGIEGVEKGDIVTVVG
                     KEGKEEITIEEIADLAHTIPHEVLCSLGKIKNRLIYGNHCSLAE"
     gene            9618..10064
                     /gene="tsaE"
                     /locus_tag="PK016_05250"
     CDS             9618..10064
                     /gene="tsaE"
                     /locus_tag="PK016_05250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HXL02508.1"
                     /translation="METIAHWQSKSEEETRKWGEKLAKDFLHPGDLVIVAGELGVGKT
                     QLVKGIAKGLGIKEEIKSPSFSLVHEYRKGDFTFYHLDFYRLEEEEEVIDLGWEEYIE
                     DEKGIVVVEWGDKFPSIFPPPFFWVRLSYGKEDRRDIEVCYDSCSG"
     gene            10045..10713
                     /gene="tsaB"
                     /locus_tag="PK016_05255"
     CDS             10045..10713
                     /gene="tsaB"
                     /locus_tag="PK016_05255"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HXL02509.1"
                     /translation="MILALDSSTPWLSIALASPGEVFFHLTHYTQKDHSRWLIKHLSL
                     LQKELDWEKDLEAILLGLGPGSFTGVKIASMVAKSIAYSLEKPLGGFSTLEVIAHQVP
                     ENLAQDYQILLPVIKHKRKEVFWMELPPQFQKSFPSPEIRIGSKEELAERYRGRNVLV
                     VTPWVDIAEFLGSEGFSYFAPQKSLPEARGLIELYRWREDKAMVNRENIFTLVPFYGS
                     KVWG"
     gene            10691..11173
                     /gene="rimI"
                     /locus_tag="PK016_05260"
     CDS             10691..11173
                     /gene="rimI"
                     /locus_tag="PK016_05260"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HXL02510.1"
                     /translation="MAQRSGDSETYLWEEGDFKISRMRPYHLPQIMAIERSSFPQPWS
                     YSLFLSELSNRAATYFVAFFQSKIIGYLGLWTVWGEAHITTFAIHPAYRGEGFGKKLF
                     GYALDYAKSQGCREVLLEVRSSNVKAQNLYRSFDFKRIGIRKHYYADGEDAWVMKKVF
                     "
     gene            11187..12209
                     /gene="tsaD"
                     /locus_tag="PK016_05265"
     CDS             11187..12209
                     /gene="tsaD"
                     /locus_tag="PK016_05265"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HXL02511.1"
                     /translation="MIHVLGIETSCDDSCVAIINDKYQVLSNVVSSQTEFHRRFGGIV
                     PEVASRKHLEYLPLVLDQALRNAHFGLDNIDFVAVTRGPGLLGSLLMGVCLAKTIALF
                     KDKPFLGVNHLEGHLFAIRLEYPEVQPPFLALIVSGGHTEIIAVRDWGKYCHLGSTRD
                     DAAGEAYDKVSKFLNLGYPGGPIIDRLSEGAQCDRFQFSAGLEDDDTLDFSFSGLKTA
                     VVRYYSSLQEEEKKDKELIRDLVASFQESVARVLVNKTFKALEVQGFSRIVVGGGVAA
                     NRHLRAKMEKEGKKRGVQVFFPSPSFCTDNGAMVAACGLFHWERGRRDSWEMEPDPQL
                     PLISIE"
BASE COUNT         3496 a         2133 c         3225 g         3561 t
ORIGIN      
        1 ctccagcgta attgttcaag ctttttctta tctatttcgt tttttaaact ttctgggggt
       61 tataaggggg tttaccccct catagttctt tttgtcattc ccgaaattct taattgggaa
      121 tccacgcttt taaataattt aggcccctga tagaggcgtt caggggtgac gaagaagaaa
      181 agacaccctc accctaaccc tctcccttca agggcgaggg aagaaatatg tggggttctt
      241 ccattgaagg cgagggagag ggaaagatga ggcaaaggag agggaaaaag aattcgaggt
      301 ttccctagtt ctgtcattcc cgaaattttt tatcgggaat ctactgaggg agatgttttt
      361 tctccctcag ttgttcttgt ttgttaggtt ttatgttttt tgggggatta taaaggggct
      421 gtgccccttt ataatcttta taatggattt tgcggtaaac tttaaaagga tgataattta
      481 actctaatct ttaattaagg agctcagctt cttgacaact cttcagggtt taatttaaaa
      541 tgccagtggt ttttgatttt tcttaaaaag gtggaggaag ttaggatgtc aaccaagact
      601 tatgtcccag atgtggaaaa aatagaaaag aaatggtatg tagtagatgc cgaaggtcaa
      661 gccctgggaa gattggcttc tcagattgcc cagattctaa tgggtaaaca caaaagtatc
      721 tatacccctt ttatggatgt gggagatttt gtgatagtgg ttaatgctga gaaagtaaaa
      781 gtgaccggca aaaaaagaga gcagaagcta tatcgctggc atactggtta tccgggagga
      841 tttaaatctg aaaccctagg ttctcttctg aatcgccgac cagaagaagt tattcgccga
      901 gcagtgtggg gcatggttcc tcacaatcgc ctgggaagga aaatgattcg caagcttaaa
      961 gtttattctg gtccttctca tcctcaccag gctcagaatc ctattccatt gaaagataag
     1021 gagtgaataa agtggcagta aatcccaaat attatgctac tggtagaaga aaaacctcag
     1081 tggccaaagt atggcttact ctggggagcg ggaaaatagt ggtaaatggc cgcgattttc
     1141 gagattattt tcctttgccg gcgtggcaaa tattgctaca aaagccgctg gctttaaccg
     1201 gtagcgaagc tcgatttgac gtggaggctg aagttgtagg aggaggcttg agtggccaag
     1261 caggagctct ggctcacggg attgcccgtt ctttagctct gatagatgaa aatttgcggc
     1321 caactttgaa aaaagctgga ttactcactc gggacccccg aatgaaagag cgcaaaaaat
     1381 acggtcaaag aggggctcga gctcgcttcc agttctccaa gcgctaaagt tttctttttg
     1441 cccccgatag ggggcttttc tccattttct tgccaccata aactcgttat cttttagaat
     1501 atggggaaag aaaggattaa aagtggccgt cggtgcttat ccccttctgg aaatcgattt
     1561 gagcataata gagagaaacg taggaattat tctcgaaaag tgtgctagct ggcacctgca
     1621 tccggtagta gtaaccaaag gttttttagc tgactcccct ctcgtttatc ttttttatcg
     1681 ctcagggata agaagttttg ccgattctaa tctggataat ttgcttcgta gtaaaaagtt
     1741 gttaccctcg ggagtgcgac ttttactcat tcgtcttcct atgaaagaag aggtagaaga
     1801 aattgtagcc caggagataa taccttttgt ttcccactgg gaaatggtga aagcactcaa
     1861 cgaggaagca gaaaaaaagg ggaaagtttt gcctctcttt ttagctttag aaagtggtga
     1921 tgctcgggaa ggttttctcc ctgaagagat agaagaaatg ggagataaaa ttggtaattt
     1981 ttcccatatt aaagtggagg gaatttgctc tactttagca tgtctcagcg gaattttacc
     2041 tgatgaggag atattaaaga gacttgtggc ttttaagagg ggatgggaag aaaaatgggg
     2101 cagaaaaatt atcctctctg tgggagggac tacttttctc tctctgtggg aaaaagagaa
     2161 ggaaataaga ggagtagatg aaattcgctt aggagaagct cttctttttg ggaacgatat
     2221 cagccgggga agaagtatag agtggttaga aggaggagca tttactctta aagcagagat
     2281 agtggagata aaaagcaaag aacctgatag gggcaaaaaa acaggttttg atgcttttgg
     2341 agaacctaat ttttcactag agaggggaaa aagaaaaaga atgattctgg ctctgggaaa
     2401 acaggatatc gatgagcatc aattatattt ttccgatgag aatgctaaaa taataggagc
     2461 tacaagtaat tacctggtca tggatataga ggaaagtgat agagtttatc aattaggaga
     2521 agtggtacaa ttccgggcag gttacggagc agtattgcgt gcttttcttt ctccctatgt
     2581 gagaaaggtg taccactggt aggaggaagg atatggataa aaaaagattt cgaggtagag
     2641 actttttaga cctggcagat tattcccggg aagagcttca ctacctttta gacactgctc
     2701 gtgagctaaa aaggatggat atgatggggg taagagaaag taaacccctg aaaggtaaaa
     2761 tagtagccct tctttttgaa aagccttctc tgcgaactcg ggtttctttc gaagtggcag
     2821 tacgacacct ggggggagaa agtttttacc tggggcctca agaagtagga ttgggacagc
     2881 gagaggcagt gaaagatgta gctcaggttt tagatcggat ggtggatggc ctggtggttc
     2941 gcactttcgc tcatgaaaac cttctggaaa ttgctcagtt tgcttctatt ccagtgatta
     3001 acggtctttc tgacctttat cacccctgcc aggtgttagg tgacctttta actctggagg
     3061 agaaaaaagg tagtctccgg gggataaaaa cctgtttttt aggagatggc aacaatgtct
     3121 gtcactcctg gataaatgcc gcttctattc tgggactaga gcttacggtg agcactccag
     3181 agcactatcg accctcttct cgcatctgga aagatgcaca agagagaggg aaagagagtg
     3241 gtgctcaaat aatctatgag gttgaccccc aaaaggcgat agaaggagca gaagtggttt
     3301 atactgatgt gtgggtcagt atgggtaaag aggcggaaaa agaagaaaga aaagtctttt
     3361 tacaacccta tcaagttaat agccatcttt tatctaaagc ttcccctgac tgcatagtta
     3421 tgcattgcat gcctgctaaa cgaggagaag aaattaccga tgaagttatg gatgggccac
     3481 attccgtggt ggtagatgag gcagagaatc gccttcatgc tcagaaagct cttttggctc
     3541 taattttgag ttaggaggaa gcctggttgg ctccagcttg gctgtggcgt gttgtaccgg
     3601 aaatcataat actgtattat cttttttatc gtctgggtag cttgctacgg ggaacttctc
     3661 tttttttgtc tttcaaatgg ctaccccttt tttttctcct ggctggtact tccaattttt
     3721 taggattccg agaactcaag tttttttggg aattgttaat ttttacttac ctgggaggaa
     3781 gcttgatagt ttttcagcct gagctccggc ggattttttt taatcggatt caccaccggg
     3841 gagagccagt cagagattat ttttttcaga atgaggaaag tagaagcgag ctcattaaca
     3901 acatcacttt agcctgtcaa actctctccc gcaaaaaaat aggagctttg atagttttgg
     3961 agcgtaataa taatttacga gattttatcc agactggtgt agctatcgat gccttttttt
     4021 ctcctgagct tttgttctct ttatttttac ctgagtctcc tcttcatgat ggtgctgcta
     4081 tcttgaggga aaatagaatt gtggcagcag ggtgcattct ccctttaaca gagagtactg
     4141 aggtaaagaa actggtggga actcgccacc gagcaggaat agggataact gaacaaactg
     4201 atgccctggc tttagtaatt tcggaggaga ctggtaaaat atctttagct gtacatggta
     4261 aaatggcctg ggatattgaa ataggagcat tgaaaaaaat gctaaaaatt ttgtataaaa
     4321 gggtataaaa agtgaacgtg gtagagatac ttaaaaagag atggcagaat tttgagcgga
     4381 gatggaaaag agataaggaa attagaatct gggcttgctt gttagctttg agtttctggt
     4441 tttttctgtg gagcgaaaga ctgtaatacc gaaataaaaa gacgaaaggt gtaaagaaag
     4501 ggggattatt tcttgaataa ctttgccagc aaaaatatta gaaatgtagg tcttttttct
     4561 catgccgggt caggtaaaac cacgctgagc gaggctctgc ttttcaatgc tggactgctg
     4621 tcccgccggg gtagagtgga ggaaggtaac actgtctccg attggcagcc cgaagagata
     4681 aaaagaggta tatctattga cctttctatc cttccctggg aatggaaagg ttgcaaggtt
     4741 aatttaattg atactccagg atatgctgat tttgtaggca atgttttagg ggcagtaaga
     4801 gcggtggata gcggggtggt gctattgtgt gctacctcag gagtggaggt agggacggaa
     4861 aaagtttggc aatatctctc tcaggagaat ctgccagtca ttttttttgt taaccggatg
     4921 gatagagaag gagccaattt tgatgaggca gtggaggagc tgcgaaaaaa aataagcccc
     4981 agagtcactc ctctttttat cccggtgggg aaggaaagta acttcacggg agtagtagat
     5041 ttactccatt ttcgagctct ttactttgga aaagagggaa aagtggagga gggagaaata
     5101 ccggaggaga tgaaagaagc ggtacagaat gcccgagaaa atctggtgga aaatatagct
     5161 gagaccagtg atgagcttct agacctgtat cttgagggga aagaaatctc tccttctcaa
     5221 ctggaagaga ctttacgaat tgccatccgg gaacgcaaaa tttttcccgt tctctgtggt
     5281 tcagggggag aaaataaagg ggtggatgtt ttagctgact ttttggtaga ttttgctcct
     5341 tcaccactcg agcgtcctcc ggtgcgggga attaatcccc tcactaaaga agaggaagag
     5401 agaaaatgtg atgaaaacga gccttttttt gctctggttt tcaaaatcat cagcgaccct
     5461 tatgtgggga aactggctct tttccgggtc ttttctggaa aaatatcttc cgactctcgt
     5521 ctctataatg cctctcgagg agtggaggaa aagatagggc aactcctctt tttgcgggga
     5581 aagaatcaag aaccggtaaa agaagtagga gcgggagata taggagtgtt ggcaaaaatt
     5641 ggtgaggttt atattggaga caccttggcc gaccgggaac accccattgt tttccctccc
     5701 atttcctacc ctgaacctac ttacatggca gctatcaggc ctttaggtaa aggagacgaa
     5761 gaaaaaatca gtcaggctct atcccgactt atggaagaag accctacttt ccgcttgtat
     5821 agagacccgg atactaaaga agatatcgtt tatggaatgg gagatatcca tctggatgtg
     5881 ctagtggaga aaatgaagag gaaatttgga gtggaggttt ctctcactat tcctcaagtt
     5941 ccctataaag aaactattaa gaaatctact aaagcagagg ggaagtataa gagacaatca
     6001 ggaggaagag gacaatatgg acacgcattt atagagttag aacctctacc ccgggggcag
     6061 ggctttgagt ttgtggataa aatcgtagga ggagttattc ctcgaaatta catcccggca
     6121 gtggaaaaag gggtacagga agccatgcaa gaaggtattc tggctggata cccagtgata
     6181 gatgtgcggg ttactgtttt cgatggttct tatcatccgg tagattcttc tgatatggct
     6241 tttaaaattg ctggctctat ggcattcaaa aaaggggcgg gtgaagctga gccggttctt
     6301 ttagaaccga taatgaacgt agaagtgata gtgcctgaag agtatatggg agatgttatc
     6361 ggagacctga atagccggag agggaggatt ttaggtatgg atccggaaga tggttatcag
     6421 aaaattaaag ctctggtgcc tatggcagaa cttttccgtt actcggtgga tttgcgttct
     6481 atcacgcagg ggcgaggata ttttactatg aagttttccc attatgaaga agtgccagcc
     6541 cagattgctg agactataat tgaaaaagct aagaaggaaa aagaagcagt ttcgtgaggc
     6601 agagaatttt agtgggagtg gatatagtag aaatagagag agtagaaaaa gcggtagaga
     6661 gatttgggga tcgctttttg aataagctct ttcaggaaga agagctccgt ttttattctt
     6721 ccccgaagag gcaacgagaa ggaatagcag cccttttttg tggtaaagag gcagtgaaga
     6781 agcttttttt atctaaaaat ttccttcttc cttggcgaga ggtcaaaata gaacacactt
     6841 cccggggaaa accagaagtt cggctttctt ctcgagttcc tgcttttttt gaggagattg
     6901 atatttctat ttctcacagt cgccagttcg ttgtggcggt ggctataggg gtagaaaaag
     6961 aaggtgaaag tgttggaatt ggttagctca gaaaccatgc aaaagctgga gaagactctc
     7021 tctgaaaagt atggagtgga tactttacta ttgatggaaa atgccgggcg gggagtggcg
     7081 gaggagataa taaaaattat ccagagtcac tctttctcta aagtttatat actgtgtggt
     7141 atgggtaaca atggtggaga tggtttagca atagctcgcc gccttcgcct tcgtctcccc
     7201 tccctttctc ttttaattta ccttttaggt aaaggggaga atctaaaaaa agatgctcgc
     7261 tttaattatc aggtggtgaa aaatttatcc cttccgcttg tagaagtttc ttcctcacag
     7321 gaagtggaat tagagagagg ggctctcgta gtggatgctc ttttagggac gggtattacc
     7381 cgagaagtgg aggggatata ccgggaggta atagaaaaaa taaatgcatc gggtaacttt
     7441 gtggtagctg tggatgtccc ctcaggagtg gaggcaagcg atgggcgaat aatgggagaa
     7501 gcagtgagag ccaacctcac tgttacttta gggctgataa aaaggggatt atgcagcttt
     7561 ccggggcgag aatacgtggg aaagttaaag ctggtagata ttggtattcc cctccctttg
     7621 gaagaagccg gattcaaagc tgatgggttt ttgatcactc catctatggt aaaagaaatg
     7681 cttccacaga ggcactggga tactcataaa atttctgccg gagttttagg agtaatagct
     7741 ggctcttctt ctcttctggg ggctggcctt ttagtatgtt cgggagggta tcgctcagga
     7801 gctgggatgg tagtctggcc tctcgatttc tctctttcac ctttagtaaa actctttctt
     7861 cctgaagtgg tgagtgttaa cctttcttct tcgggggagt ggcattattc tttatcccac
     7921 tgggaagtgg tagaaaaagc atttcaggat agaaaatgtg gagctttggc tgtggggccg
     7981 gggatgggaa ctaacccttc tacctctctc tttttggaaa aagtgttaga gaatttccgg
     8041 ggggtactgg atgctgacgc tttgaatatc atttctcaga atagagataa ctggaagggc
     8101 cgccttgcaa gctgggttat tactcctcat tctggagaga tggcgagaat tttaggcgtt
     8161 tcagtgccgg aagtgaatga aaataggatt gctgccagct tagaagcttc ctctctttta
     8221 ggatgtgtga cagttttaaa aggggcagga acagttatag cttctcctca aggggaggta
     8281 tttttgaacc ctacagggac gccagttctt gccagtgcag gaagcggtga cgttttgaca
     8341 ggaatcatcg ctgggcttat ggctcagggg atgagccctt taaatagcgc aataagtgga
     8401 gttttcctcc atggactggc aggggagctc ctggaaagag aggggaaaaa gcagatttta
     8461 tctcgggaaa tagctgaaaa actgaacgaa gctcgaagga tggtagaaaa tggagaatat
     8521 actctttcct ttctggatgg agattgatgc tcagaattta catcacaatg tagaggagct
     8581 gaagagatgg tcatcttctt ctcttatggc agtagtaaag tcggaggctt atggtcatgg
     8641 atgggaagcg gtaaaccttc tttatgatta tgggttgaga aaatttggag tggcttcagt
     8701 agaagaagcg cacgagttga ggaaaagagg cctaagaggg gatatatatc ttttaggggg
     8761 gttttttact gaagaagcag aaacgatttt agaagaagaa ttaactccgg tagtttcttc
     8821 agataaggaa ctacaggctc tggaagaggt ggccaagaaa tctggtaagg ttataccttt
     8881 tcatctaaaa gtagataccg ggatggggag aatgggattt ttacctcaag aaataaatga
     8941 caggtggtgg aagaagtggg gaaataactg tcatctttct ttgcagggga ttatgactca
     9001 ttttgccagt gctgaatctg acccggtgta tactcgcttt caatttgaca actttctttc
     9061 ttttttgcat cggcttccgg aaaatagtcc ttccctggag agacactgct gtaatagtgc
     9121 tgcctttctt tcttttccgg aaatgcatct cgatttatcc cgggtgggga ttgccttctg
     9181 gggtgtttct ccctggggga gaaagtcagc tctatcgctg gatttaaagc cggtaatgag
     9241 aataaaagcc cgagtaaaac aggtcaaaac tcttcctcct gggaaccggg tgggttatgg
     9301 agggactttc actacttacc gagaaaccaa actggcagcg atatctattg gctattctca
     9361 gggtctgttt cgcagtcttt ccaaccggat ggaggtatta ataaagggga aaagagcgcc
     9421 atcccgggga aatatttcta tggaccagat gactgttgag gtgacaggaa ttgagggagt
     9481 ggaaaaagga gacattgtta ccgtggtagg aaaagaaggg aaagaggaaa taaccataga
     9541 agaaatagcg gatttagctc ataccatacc tcatgaagtt ctctgtagtt taggtaaaat
     9601 aaaaaatcga ctgatttatg gaaaccattg ctcactggca gagtaaaagc gaagaagaaa
     9661 ctcgaaagtg gggagagaaa ttagctaaag attttttgca ccccggggat ctggtgatag
     9721 tagctggaga gctgggggtg ggtaagactc agttagtaaa aggaatagct aaaggactgg
     9781 gtataaaaga agagattaag agcccctctt tctctttagt tcatgagtat agaaagggag
     9841 attttacttt ctaccattta gatttttacc gtctggaaga ggaagaagaa gtgatagatt
     9901 taggatggga agaatatata gaagatgaaa aagggatagt ggtggtggag tggggagata
     9961 aatttccttc tatctttccc cctccctttt tttgggttcg cctgagttat ggtaaggaag
    10021 acagaagaga tatcgaggtg tgctatgatt cttgctctgg atagcagcac tccttggctt
    10081 agcatagccc tggcctcccc cggggaagtt tttttccatc tgactcatta tactcaaaaa
    10141 gaccattctc gctggcttat taaacatctg agcctgctgc agaaagagct cgactgggaa
    10201 aaagatttag aggcgattct tttaggttta gggccgggtt cttttaccgg ggtgaaaatt
    10261 gcaagcatgg tggcaaaaag tatagcttat tctctggaaa agcctttagg tggtttttcc
    10321 actctggaag ttatcgccca tcaagttccc gaaaatttag ctcaagatta ccaaatttta
    10381 cttcccgtaa taaaacataa aagaaaggaa gttttctgga tggaattgcc gcctcagttt
    10441 caaaaatctt ttccctctcc agagattcga ataggttcta aagaagaact agcagaaaga
    10501 tacagaggta ggaatgttct cgtagttact ccctgggtgg atatagcaga atttttaggg
    10561 agcgaaggtt tttcttattt tgcaccccag aaatctttac cggaagcccg gggattaata
    10621 gaattgtatc ggtggagaga agataaagcg atggtaaatc gagaaaatat ttttactctg
    10681 gtgccctttt atggctcaaa ggtctgggga tagcgaaact tatttatggg aagaaggaga
    10741 tttcaaaatc tcccggatgc ggccttatca tttgcctcaa attatggcca ttgagcgaag
    10801 ttcttttcct caaccctggt cttattcttt atttttgagt gaattatcca accgggcagc
    10861 tacttatttt gtggcctttt tccagagtaa aattattggt tacctgggtc tctggacggt
    10921 atggggggag gctcatatta ccacttttgc cattcatcct gcttatcggg gagaagggtt
    10981 tggtaaaaag ctttttggtt acgctctgga ttatgctaaa tctcaaggat gcagagaagt
    11041 tttactggaa gtacgctctt ccaatgttaa agcgcagaat ttgtatcgta gttttgactt
    11101 taaaagaata ggaatacgca aacattatta tgctgatgga gaagatgcct gggtgatgaa
    11161 gaaagtcttt tagaggagga tgtttattga ttcacgtttt gggtatagaa acgagctgtg
    11221 acgacagctg tgtagcgata attaatgata aatatcaggt tttatccaat gtagtatcca
    11281 gccagactga atttcatcgc cgttttggag gaatagtccc ggaggtggct tctcgtaaac
    11341 atttagagta tctccctctg gttttagacc aggctcttcg gaatgctcat tttggtttag
    11401 ataatataga ttttgtggca gtgactcgag gtcctggcct tctggggtct ttgctcatgg
    11461 gggtgtgttt ggctaagacc atcgctcttt ttaaagataa accatttctc ggggttaatc
    11521 atctggaagg gcatctcttt gccattcgtc ttgaatatcc agaagttcag cctccttttt
    11581 tagctttgat tgtttcaggg gggcacacgg aaatcattgc tgtccgggat tggggaaaat
    11641 attgtcattt aggaagtaca agagatgatg ctgctggaga agcttatgat aaagtatcca
    11701 aatttttaaa tttaggttat cccggaggtc ccatcatcga tagattgagc gaaggtgctc
    11761 aatgcgaccg tttccagttt tctgctggtt tagaagacga tgatactctg gatttcagct
    11821 tcagcggatt aaaaactgca gtagtgcgtt attattcttc cctccaagaa gaagaaaaaa
    11881 aagataagga gctcatccgg gatttagtag cttcttttca ggaaagtgta gcgagagttc
    11941 tagtaaataa gacttttaaa gctctggaag tacagggttt ttcccggata gtggtgggag
    12001 gaggggtagc ggccaatcgg catctgcgag cgaagatgga gaaagaagga aaaaaaagag
    12061 gggtgcaagt tttctttccc tctccttcct tttgtactga taatggggct atggttgctg
    12121 cctgtggtct ttttcactgg gagcggggga gaagagattc ctgggaaatg gaacctgacc
    12181 ctcagcttcc cttgatttct atagagtaaa gactgaagag aggggattat cactctcacc
    12241 ctgctcctct tcccggaagg gagagggaag ctttttctcc ctcatagttc tgtttttcct
    12301 ccctcagtag tatttgtttt tatgtatttt ttcgtaacta ctcaggggcc taaatttttg
    12361 ttgaatccta ttgacagttc cttgaaagcc gtgaatacta tgcgcactat gaaaa
//
LOCUS       DAOSVE010000047        12235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_3005_length_12235_cov_68.900328, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000047 DAOSVE010000000
VERSION     DAOSVE010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 12235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12235
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3005_length_12235_cov_68.900328"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="PK016_05270"
     CDS             <1..1152
                     /locus_tag="PK016_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXL02512.1"
                     /translation="EEATNFEELINYCASIAKDLLNFATSYIMDFGEDSLSSQIEATA
                     NKLSWFNDYSTFLDRFNKFSKNLSTPLEKSNSLISDFEEKWEGLLRASLNGEKVSVRE
                     LDELYNVIKTDYVNMVGSHISVVSRKAGDLMDTMPYRELKKDLNSVDKELFRINQIWT
                     QKTRHYFLRWVRNSIVVAGVAALITTFICSLAAYPFSRMRFWGRKYGILAMLLIQMFP
                     SAVYMIAVYSLLNLLGRFIPFLGLDTLTGLTFIYLGNIAYNVYLIKGYYDTIPNSLEE
                     SAMIDGATRFQTFYKIVIPLALPILTVVVILTFMNIFNEFVFARIILQDAKNYTYAIG
                     LWTFSSGPYQTEWGLFTAAALLGMLPMTVLFLSLQKYIVSGLTKGAVKE"
     gene            1155..2999
                     /locus_tag="PK016_05275"
     CDS             1155..2999
                     /locus_tag="PK016_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HXL02513.1"
                     /translation="MVKTASLIIMIGLGSLMIMGFTGSQIMANPVENLPSEFIKGVDV
                     SMLYEVEANGGKFYDNGVEKDALQVLKDYGVNWIRIRIWNDPTDENGKPLGGGNCDYV
                     KMTELARRAKALGMKVLVDFHYSDWWADPGKQNKPKSWKNLAGANLANAVVDFTHEVL
                     KYMEDNNALPDMVQLGNEVNNGFLWPDGRIAGESGFDSFVALFNAGAEAVRKINKDIK
                     IALHLAEGGNSDLFEWFLENVIKRGIDFDVIGISYYPYWHGTLEELQANLNHIEKYGK
                     EIAIFETAYAWTLEDADGHPNIFGSDMEITGGYKATPQGQTTAIREVMEAVAQITSGK
                     GLGIFYWEGCWIPVQGAGWKTGEGNPWENQALFDFEGNALPSLWVFNPIYGENHIEPQ
                     IREIISPLDIKISPGEIPTLPEKIKVLFSDDSIKSLPVKWEDIKKELLTSPGEFQVKG
                     VIEKPEIEVYAKVAVVERENFILNPGFESGNFNPWIVEGNKNAVKVVRASPPQNAKSG
                     EYAVNYWLDSPFQFEMYQVVEGLKPGRYQVSFWIQGGGGENLIRLKVSNYGGEEKFID
                     IINTGWLNWSNPVIRDINVTTGQIKISVIGDGNPGNWGWIDDFELKEE"
     gene            complement(3069..3818)
                     /locus_tag="PK016_05280"
     CDS             complement(3069..3818)
                     /locus_tag="PK016_05280"
                     /inference="COORDINATES: protein motif:HMM:NF023178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HXL02514.1"
                     /translation="MEFSSKWLVLELLLFSHGVADFILQSNKMIEDKARGKWTTYISH
                     FFIVWGLSVLFLHPFLSWQVVGVLLCLALTHLMVDEIGYRITPPHKIDSNPLGFGLDQ
                     LFHLIFILLAWQWLGYLPHLSLSGFYSEVMTSAWIEKLTSVMVVGNVYLYVVFGGAIF
                     IGKVMRSLPQEVRKYEITGMGRYVGILERIIILTFILYKAVAGIAFVLMAKSLTRYQE
                     LDNKSFAEYYLVGTLWSTALALIGGLFFYLR"
     gene            complement(3819..4484)
                     /locus_tag="PK016_05285"
     CDS             complement(3819..4484)
                     /locus_tag="PK016_05285"
                     /inference="COORDINATES: protein motif:HMM:NF027589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HXL02515.1"
                     /translation="MFYPEGKFAVLTADVIGSRKLKDRSLSRKQIEKAISELNTVRKA
                     FIVSKFSLLRGDEIQGVIFPPQEAIVMARFLRFYLKPVRVRIGIGIGDIATEIDENNP
                     WNMDGNAFHRARKALEEIKISRQPVTFINSAEEDFDYNLNVILALMDVISNRWTDGQW
                     EAIHTYEQMGTYQKAAQVLKVKPQNVAKRCQAANWKVFSEAEKHVQKLISLKFPTGNE
                     EEN"
     gene            5426..6532
                     /gene="ugpC"
                     /locus_tag="PK016_05290"
     CDS             5426..6532
                     /gene="ugpC"
                     /locus_tag="PK016_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HXL02516.1"
                     /translation="MADVTLKSVVKRFGEVVAVNKVDLDIKNEEFLVLVGPSGCGKTT
                     TLRMIAGLEEVDEGEIYIGDTLVNDVPPKDRDIAMVFQNYALYPHMDVYNNMAFGLKL
                     RKFPKDEIDRRVKEAADILGIHELLNRKPKQLSGGQRQRVAVGRAIVRQPKVFLFDEP
                     LSNLDAKLRVQMRAELSKLHDRLKTTMIYVTHDQIEAMTMGDRIVVMKDGIVQQIGTP
                     LDLYNKPQNKFVAGFIGTPSMNFLDVVVKKEGDTLYLDGESFKIKIPVEIKSLVEPYV
                     GKEVTLGVRPQDIYDYSFSQDMAEVDGNVIDTTVDIVEPLGSEQTLYLVTGPHTLVAQ
                     LDMRTQTIVGETLKVVVNISKIHIFDIETEKAIV"
     gene            6638..7627
                     /gene="gap"
                     /locus_tag="PK016_05295"
     CDS             6638..7627
                     /gene="gap"
                     /locus_tag="PK016_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HXL02517.1"
                     /translation="MKVGINGFGRIGRLVFRRILEEGDVDVVAVNDLTNAKTLAHLLK
                     YDSVHGTICNQVEAKDDALVVDGKEIKVLAQKDPAQLPWKNLGVDLVIESTGKFTARD
                     GASLHFQAGAKKVVITAPAKGPDVTIVMGVNHKVYNKDAHNIISNASCTTNCLAPVVK
                     VLNDHFTIQKGFMTTIHAYTNDQVILDFPHKDLRRARAAALSMIPTTTGAASAIGEVI
                     PELKGKLDGMAIRVPTPDVSIVDFVAVVEKETSAEEVNSVLKQAAEGELKGILAYCDE
                     PLVSADFLHDTHSSIVDALSTKVYGNLVKVLAWYDNEWGYSCRVVDLVKYVMK"
     gene            7638..8408
                     /gene="tpiA"
                     /locus_tag="PK016_05300"
     CDS             7638..8408
                     /gene="tpiA"
                     /locus_tag="PK016_05300"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HXL02518.1"
                     /translation="MLRIPIAAGNWKMNKTSQEASAYAEAFLPQIKDFKKEIEILICP
                     PFTALSTLASYFRNSPVKLGAQNMHWEEKGAFTGEVCAPMIKEWGCQYVILGHSERRH
                     IFRETSQEVGKKVKKALEWGINPVLCVGETIEEKEKGETENIVEEQLLLGVADVPDAK
                     ASSIVIAYEPVWAIGTGKAATPKDARGVISFIREKLSSRFSSEVSENIRILYGGSVSP
                     ENVFDFLREEEVDGTLVGGASLDPLKMVKIVEETLRAY"
     gene            8428..8652
                     /gene="secG"
                     /locus_tag="PK016_05305"
     CDS             8428..8652
                     /gene="secG"
                     /locus_tag="PK016_05305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HXL02519.1"
                     /translation="MEWWMIVQIVVCAALILVVMFQPRKAGLGGGIFGGMTRADRSSK
                     FRSLPVLSKLTVVLGVAFLLVSFLFSFLLA"
     gene            8665..9705
                     /locus_tag="PK016_05310"
     CDS             8665..9705
                     /locus_tag="PK016_05310"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HXL02520.1"
                     /translation="MIENRRVLTLEEVEHLKIDPERGFYSATPEEIKKGYTTDIYFVS
                     AQEILRHLNLKDTIVTAEIFPRGSGVMCGVPEVLNLLSSTPVEVWSLSEGEEFSPKEV
                     IMRIKGPYSAFGVFETPILGILAHSSGWATAARECKLAAGEKVVLSFGARHVHPAVAP
                     VMERSAIVGGADGAACVLGAKLAGKEPGGTIPHAAILIVGDTVVAAQVFQEVIPPHYS
                     RVILVDTFKDEAEESLRVARVLGKNLEGVRLDTPEERGGVTPGLVEEVRVRLDQAGFS
                     QVKIVVSGGLNPARIQTLKDKVDIFGVGSYISGHSPIDMTMDLKEIGGEKIAKRGRLP
                     GIIPNPRLKKIK"
     gene            9722..11557
                     /locus_tag="PK016_05315"
     CDS             9722..11557
                     /locus_tag="PK016_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL02521.1"
                     /translation="MKKYGVFLLAVLVLVGGMSFAEIKNPDTYIYLSIGEPDTLDPHY
                     AYDTASGEAIGMVYDNLIAYKGDSVKEFIPRLSLEVPSLENGLIKDDGKTYVFPLREG
                     VKFHNGSDLTPEDVEYSFERGILTDPYAGPMWMIIEALFDYQTIEDMVGDKLQIAWSD
                     MVNEDGTLKSEEYASKLVDFYHQYIDPAIEVEGQNVVFHLVRPFAPFLSILSQNSSWS
                     AIMDKETCVSLGLWNGEPDGWWKYHTLKKEESPLYEKAIGTGPFVLVEWDRAQQKLTF
                     NRNDNYWGEKPKIAKAVIWGIDEWSTRRAMLEAGDADQIYTPLQYLEQVKGMPGVTIE
                     EEARITITALHFNWTVEKGSQYLGSGQLDGEGIPPDFFSDVHIRRAFSYAFDYDTFIE
                     QVLQGYGFRIPSVIPKGLLGFNEGLPMYNFDLEKAKEEFQQAWNGEVWEKGFKLTLLY
                     NTGNEARQTACEMLKEYIESLNPKFKIEVQGVQWPTYLDAYRSGQLPAFIIGWLADYP
                     DPHNFVFTYFHSNGVYGTTQGQAFLDFARENLDSLVEKAIAEVDDEARQKIYEEIQTI
                     AYENALGMTLYQPQEIYPMRDWVKGWTFNPMRPGEVNWEGVYKEE"
     gene            11617..>12235
                     /locus_tag="PK016_05320"
     CDS             11617..>12235
                     /locus_tag="PK016_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL02522.1"
                     /translation="MIFYIIRRLLFLPLTLLGISILVFLMIGLLSPYQRLATFINSPE
                     RLKGADIDKLVEFYGLNDPLVVQYGRWLKNLFQGNLGWSYVGKAPVYDALVERFPYTL
                     ELALFSIFPIIFLGIQLGVISAVNHNRPLDHGLRIFAVVGWSFPDFVFGLFILMIFYS
                     VLGWFPPGNLSLWAEEVVKSSAFHNFTGMTTIDALLNGRLDIFWDA"
BASE COUNT         3679 a         2174 c         2841 g         3541 t
ORIGIN      
        1 gaagaagcta caaacttcga ggagctcata aattactgtg catccatcgc caaggacctc
       61 ttaaactttg ctacctcata catcatggat tttggtgaag atagcctgag ctcacaaata
      121 gaagccacag caaataagct gagctggttt aatgactata gcactttttt agatagattt
      181 aataaattct ccaagaacct aagcactcct ttagagaaaa gcaattcctt gatttccgat
      241 tttgaggaaa aatgggaagg cttgctccga gcttctctga atggtgagaa agtaagtgtc
      301 agagagctgg atgaactata caatgttata aaaactgatt acgtgaatat ggtaggctct
      361 catatcagcg tcgtttcccg aaaagcagga gatttaatgg atactatgcc ctatcgagag
      421 ctcaagaaag acctgaatag cgtggataaa gaactattta gaataaacca gatatggacg
      481 caaaagacca ggcactattt cttgagatgg gtcagaaact ccatagtggt agccggcgtt
      541 gctgctttga ttaccacttt catatgttct ctcgctgctt atccttttag caggatgaga
      601 ttctggggaa gaaagtatgg aattctggcc atgcttctca tccagatgtt tcccagcgct
      661 gtctatatga ttgctgtcta cagtcttttg aatcttctgg gaagattcat tcctttcttg
      721 gggcttgata ccttaaccgg tcttactttt atttatctgg gaaacattgc ctataatgta
      781 tatttaataa agggatacta tgataccatt cctaactctc ttgaagaatc agccatgatt
      841 gatggcgcta caagattcca gactttttat aagatagtga tacctcttgc tctgcccatc
      901 cttaccgtgg ttgtgattct tactttcatg aatatcttca acgagttcgt ttttgctcgc
      961 ataatattgc aggatgccaa aaattatacc tatgctatag gtttgtggac tttctccagt
     1021 ggtccctacc agaccgaatg gggtcttttc actgctgcgg cccttctggg gatgctacct
     1081 atgaccgtat tgtttctctc tcttcagaaa tacattgtta gtggccttac caaaggtgca
     1141 gtcaaggaat agaaatggtt aaaacagcta gcctgattat tatgataggt ttgggaagct
     1201 tgatgataat gggatttacc ggtagccaga tcatggcaaa tccagtagag aatttaccct
     1261 ctgagttcat aaaaggcgta gacgtatcta tgctttatga ggtagaagcc aatggaggta
     1321 aattctatga taacggtgta gaaaaagacg ccttgcaggt gcttaaagat tatggagtga
     1381 actggataag aataagaata tggaatgacc ccaccgatga aaatggaaaa ccactgggag
     1441 ggggaaattg tgactatgta aagatgaccg aattggctcg aagagcgaaa gcactgggaa
     1501 tgaaagtatt ggtggacttt cactatagtg attggtgggc tgacccgggg aaacagaata
     1561 aaccaaagtc ctggaaaaat ttagcagggg caaatttagc aaatgcagta gtagacttta
     1621 ctcatgaggt tcttaaatac atggaggata ataacgcctt acccgatatg gtgcaattag
     1681 ggaatgaagt taataacggg tttttatggc ctgatggaag aatcgctggc gagagcggat
     1741 ttgatagctt tgtagctctt tttaatgccg gggcagaggc agtgagaaaa atcaataaag
     1801 atatcaaaat tgctctacat cttgccgaag gaggaaacag tgatttattc gaatggttct
     1861 tggaaaacgt cataaagaga ggtatagact ttgatgtgat aggaatttcc tattatcctt
     1921 actggcatgg tactctcgag gagctccaag caaatctcaa ccatatcgag aagtatggca
     1981 aggagatagc tatttttgaa actgcctacg cctggacact cgaagacgct gatggacatc
     2041 ccaatatttt tggctcggat atggaaatca caggaggata taaagctact ccccaggggc
     2101 aaacaactgc aattagagaa gtaatggaag ctgtagctca aattaccagc gggaaagggc
     2161 tgggaatatt ttactgggaa ggttgctgga tcccggtaca aggagcaggc tggaaaaccg
     2221 gtgagggaaa cccctgggaa aatcaggctt tattcgattt tgaaggcaac gctctgccct
     2281 ctctttgggt ttttaaccct atttatggtg aaaatcatat agagccgcaa ataagagaaa
     2341 ttatttctcc cttagatata aaaatttctc cgggagagat tcccaccctt ccggagaaaa
     2401 taaaagtttt attcagtgat gactctataa agtctcttcc ggtaaagtgg gaagatataa
     2461 aaaaagagct tctcacttct cctggtgaat ttcaagttaa aggagttata gagaaaccag
     2521 aaatagaagt atacgcaaag gtagcggtag tagagaggga aaattttata ctgaatcccg
     2581 gctttgagtc cggaaacttt aacccctgga tagtggaagg aaataaaaat gctgtaaaag
     2641 tggtaagagc aagtcctccc cagaatgcta aatccggaga gtatgccgta aattactggc
     2701 ttgacagtcc cttccagttt gagatgtatc aggtagtgga aggcttaaaa ccaggaagat
     2761 accaggtgag cttctggatt caaggtggag gaggagagaa cctgataagg cttaaagtaa
     2821 gcaactacgg cggagaagag aagtttatag atataataaa tacgggatgg ttaaactgga
     2881 gcaatcctgt gatccgggat ataaatgtta ccaccggaca aattaaaata agtgtaattg
     2941 gggatggaaa cccgggaaac tggggctgga ttgatgattt tgagctaaaa gaagaataaa
     3001 aaaccagaat cagcaaacta ttcaaaaaag attttccgtg agttctaaaa cctctccagc
     3061 tttcctgctc atctgagata gaaaaataat ccccctatca gggctaaagc ggtgctccag
     3121 agagttccta caagataata ttcggcaaat gatttgttgt ccaactcctg atatcgagtc
     3181 aatgatttag ccatcaagac aaaggctatc ccggcaactg ctttataaag gataaaagtc
     3241 aggatgatga ttctttctaa gatgcctaca tatctcccca tcccggttat ttcatatttt
     3301 ctcacttcct gaggtagaga acgcataact ttccctataa atatggctcc accaaagact
     3361 acataaagat atacattacc caccaccatt acactggtta gcttttctat ccaggcggac
     3421 gtcattacct cagaataaaa accagagaga gataggtgag ggaggtagcc taaccattgc
     3481 caggcaagaa gaataaaaat taagtgaaat agctggtcta acccgaaccc cagaggattg
     3541 ctatctattt tgtgaggagg agtaatacga tagccaatct cgtccaccat taagtgggta
     3601 agagcgaggc acagaagaac tcccaccacc tgccaggaga gaaaaggatg taaaaacaga
     3661 accgaaagtc cccaaacaat aaaaaaatga gaaatatagg tagtccactt tccccttgct
     3721 ttatcctcta tcattttgtt gctctgcaaa atgaagtctg ccacaccatg agaaaagagt
     3781 aataactcca gcactaacca cttactggaa aactccactc aattttcctc ctcattacca
     3841 gtgggaaatt ttaagcttat cagtttttga acgtgtttct ccgcttcaga aaaaactttc
     3901 caattagcag cctggcaacg cttggctacg ttttgcggtt ttactttcaa aacctgagct
     3961 gctttttgat aagttcccat ttgttcataa gtatgaatag cttcccattg accatcagtc
     4021 cagcggttac ttatcacatc catcaaagcc agaattacat tcaggttata atcaaagtct
     4081 tcctcagcgg aattgatgaa ggtaacaggc tggcgagaaa tttttatttc ttccagcgct
     4141 tttctggctc gatgaaaagc gttaccgtcc atattccagg ggttattctc atctatttct
     4201 gtagcgatgt ctcctatccc tatccctatt ctaactcgaa ctggcttcag ataaaaacgc
     4261 aaaaatctag ccatcactat tgcttcctga ggggggaaaa tcactccctg aatttcatct
     4321 cctctcagga gcgagaattt agagacaata aaggcctttc tgactgtatt aagttcagaa
     4381 atagcctttt ctatttgttt tctggaaaga ctccgatctt ttaattttcg agagccaatc
     4441 acatcggcag ttaaaacagc aaatttcccc tccggataaa acacttgctg actccttttc
     4501 acccgtttaa gggttatatt ttgatttcac cccttaaaag gtgagaatat aatttcacct
     4561 tctctaatta taccgcagaa ctataaaggg aagtactcat tataatcgaa ctatgagggg
     4621 aaaaaagacc ccctcatagt tcgatagaac cattcaggaa taacggcaag aagacaccct
     4681 caccctaacc ctctcccttc gagggagagg ggaaaaagat gtagagctct cccttcaagg
     4741 gagagggaag gaatatgtgg ggttctttca ttgaaagcga gggaagaaaa agaaagggga
     4801 ttataaaggg gtgctcccct ttaagggatt ttttatccct cagtaatttc tgtttttttc
     4861 tgtttttctt ttaatttttt gaagggggtt atgaggggga aaatatcccc ctcatagtca
     4921 ttataattag tttttcggtt aactataaac actgctgaca atggtgtacc aattacctgg
     4981 taacggcttt ttatgggtta tcctctcagc ttttatgacc gaactatagg agtaatctta
     5041 tcataacccc ccataaagat taaaaggaac atgaaagaca aacactatct gaggaggaaa
     5101 aatgtgcctc aggtagtgtt taggttctcg ataaaaggtt ttgagaataa cagtgactga
     5161 aaagggagaa aatactttct ttaggacccc tgaaagtctt tttgtggttt cctgtgctga
     5221 tgttctttag taaattttgg taagcctctc tttttactga agtttaattt tttccagaaa
     5281 gcgaatttgc tgttatactt ttttaaattt attatagagc cctccagggg ttgcaagaga
     5341 agaataaatg ttctaaaata aagcaagtag ttccttttgt tccttttatt taaaatttga
     5401 gtaagtaaat gggaggaaaa aataaatggc tgatgttact ttaaaaagtg tagtcaagcg
     5461 tttcggggaa gtagtggcag taaacaaagt agacctggat attaaaaatg aagagtttct
     5521 ggtgttagta ggtccttctg gttgtggtaa aactactact ttgcgtatga ttgccggatt
     5581 ggaagaagtg gatgaagggg aaatttatat tggtgatact ctggtaaacg atgttccccc
     5641 taaagatagg gatatcgcca tggttttcca gaactacgct ctttaccctc acatggatgt
     5701 gtacaacaat atggcttttg gattgaaact tcgtaaattt cccaaagatg aaattgatag
     5761 gcgggtaaaa gaggcggcag atattctggg tattcacgaa ttacttaatc gtaagcccaa
     5821 gcagctctcg ggaggtcaac ggcaaagagt agcggtggga agagctattg ttcgccagcc
     5881 taaagttttc ttgtttgacg agcctctttc taacctggat gctaaattga gggttcagat
     5941 gagagcagaa ttatctaaac ttcacgaccg tttaaagact actatgattt atgttaccca
     6001 cgaccagata gaagctatga ccatggggga tagaattgtg gttatgaagg atggtatagt
     6061 acagcaaata ggaactccgc tggatcttta caataagcct caaaacaaat ttgtggctgg
     6121 ttttatcggt actccttcta tgaatttctt ggatgtagtg gtaaagaaag aaggagatac
     6181 actgtatctg gatggggaat ccttcaaaat caaaataccg gttgaaatta aatccctcgt
     6241 ggaaccttat gtagggaagg aagttacttt gggtgttcgt ccccaggata tttatgatta
     6301 cagtttttcc caggatatgg cagaggtgga tggaaacgtt attgatacca cagtagatat
     6361 agtagaaccc ttaggttcag aacagacact atatctggtt accggacctc atactcttgt
     6421 tgcgcaatta gatatgcgta ctcaaactat tgtaggagaa accttgaaag tagtggtaaa
     6481 tatctctaag attcatatat ttgatataga gacggaaaaa gctatagttt aattcagtaa
     6541 gtctcagctt gttgaggtaa agatgtccgt tgaaaagaat gcttgagcat tctttctcga
     6601 cactaaataa ataggattta agaaaggagc aacaattatg aaagttggaa ttaatgggtt
     6661 tggtagaatc gggcgtttag tttttagaag aatcttagaa gaaggagatg tggacgtagt
     6721 agcagttaat gatttgacca atgctaaaac cctggctcat ttgttgaaat acgattcagt
     6781 acatgggact atctgtaacc aggtggaggc caaagatgat gccctagtag tagatggtaa
     6841 agaaattaaa gtcctggctc aaaaagaccc tgctcaacta ccctggaaaa atttaggagt
     6901 agatctggta attgagtcta ctggtaaatt tactgctaga gatggagctt ccttgcattt
     6961 tcaagctggt gccaaaaaag tggtaatcac tgctccggct aaaggtccag atgttactat
     7021 agtaatggga gttaatcata aagtttacaa taaagatgct cataatatta tttctaacgc
     7081 ttcctgcact actaactgtc tggctccggt ggttaaagtt ttgaatgacc actttactat
     7141 tcaaaaaggt tttatgacca ctattcacgc ctataccaat gaccaggtta ttttagactt
     7201 tcctcacaaa gacttaagaa gagctcgagc agcggctcta tctatgattc ctaccaccac
     7261 tggagcagct tcggctattg gggaggttat acctgaactt aaaggtaaat tagacggaat
     7321 ggccattcga gttcctactc ccgatgtctc catagtagac tttgtagcgg tggtagaaaa
     7381 ggaaacttct gccgaggaag taaattctgt tcttaaacaa gctgctgaag gtgaattgaa
     7441 aggaatttta gcctattgtg atgaaccttt agtatcggca gattttctcc atgatactca
     7501 ttcttctatc gtggatgctt tgtctaccaa agtttatggt aacctggtta aagttttagc
     7561 ctggtatgac aatgagtggg gctattcctg ccgggtggta gatttagtta aatatgtgat
     7621 gaagtaggag ctataaaatg cttcgtattc ctatcgctgc aggaaattgg aaaatgaaca
     7681 aaacttctca ggaagcctca gcatatgctg aggctttcct cccccagata aaggacttta
     7741 agaaggaaat agagattctc atctgccctc cctttactgc tctttcgact ctggcttctt
     7801 attttcgaaa ttctccggtg aaattaggag ctcagaatat gcactgggaa gagaaaggtg
     7861 cttttactgg tgaagtgtgt gctcctatga ttaaggaatg gggttgtcaa tatgttattc
     7921 tggggcattc agagcgaagg catattttcc gggagacttc acaagaagtg ggtaaaaaag
     7981 taaaaaaagc attagaatgg ggaatcaatc ctgttctttg cgttggagaa actattgaag
     8041 agaaggaaaa aggagaaacg gaaaacatag tggaagagca acttctcctc ggggtagcag
     8101 atgttcctga tgctaaagcg tcttctattg tgatagccta tgaaccggta tgggctattg
     8161 gtacgggcaa agcggctact cccaaagatg cccgaggagt tatttctttt attcgagaaa
     8221 agctttcatc tcgttttagt tctgaagttt cggagaatat ccgtatcctt tatggaggga
     8281 gcgtaagtcc ggaaaatgta tttgattttc tccgggaaga agaagtcgat ggaaccttag
     8341 taggaggagc cagtttagac ccgcttaaga tggtaaaaat agtagaagaa actctgcgag
     8401 cctattaaag ggaaagaggt cgggaatatg gaatggtgga tgattgtcca gattgtagtt
     8461 tgtgcagctc tgattttagt ggtgatgttt cagccccgta aagcaggctt agggggtgga
     8521 atatttggag gtatgacaag agcagatcga agcagtaagt ttcgaagttt accggtcttg
     8581 agtaaattga ctgtagttct gggagtagcg tttttacttg tctctttttt attttccttt
     8641 ctattagctt agtgaagggc caaaatgata gagaatagga gggtattaac tctggaggaa
     8701 gtagaacacc tcaaaataga cccggagagg ggtttttatt ctgctacgcc ggaggaaata
     8761 aaaaaaggtt atactactga tatatatttt gttagcgctc aagaaatttt acgtcacttg
     8821 aatttgaagg ataccatagt aactgcagaa atttttcctc gagggtcagg agttatgtgc
     8881 ggagtgccgg aggttcttaa ccttctttct tctaccccgg tggaggtctg gagcctgagt
     8941 gaaggagaag aatttagtcc taaagaagta ataatgagaa ttaaaggacc ttattcggct
     9001 tttggagttt ttgaaacccc tattctggga attctggctc acagttctgg ctgggctact
     9061 gctgctcggg aatgtaaatt ggcagcagga gaaaaagtag tcctttcctt tggtgctcga
     9121 catgttcatc cggcggtggc tccggttatg gaacgctctg ctattgtggg aggagcagat
     9181 ggagcggcat gcgtactggg agcaaaactg gcgggtaaag aaccaggagg tactattccc
     9241 catgctgcaa ttctcattgt aggagataca gtagtagcag cgcaagtttt tcaagaggtc
     9301 attcctcctc attattcccg ggtaattctg gttgacactt ttaaagatga ggctgaagaa
     9361 tctttgcgag tggctcgagt tttaggaaag aatttagaag gagtgcgatt ggatactcct
     9421 gaagagcgag gaggagtaac tccaggctta gtagaagaag taagggtgcg tctggatcag
     9481 gctggttttt ctcaggtaaa aattgtggtc tctggaggat tgaacccggc aagaatccag
     9541 accttgaaag ataaggtgga tatttttgga gtgggaagct atatcagcgg tcactccccc
     9601 attgatatga ctatggattt aaaagagata ggaggagaaa aaatagccaa acgagggagg
     9661 ttgccgggga ttattcctaa tcctcggtta aagaaaataa aataaaaggg aggcgttaat
     9721 tttgaaaaaa tacggtgttt ttcttttagc ggttttagtc ttggtgggag gaatgagttt
     9781 tgctgaaatt aaaaatcccg atacttacat atacttaagt attggagagc cggacacttt
     9841 agatcctcat tatgcttatg atacggcaag tggcgaggca ataggaatgg tttatgataa
     9901 cctgattgcc tataagggag acagtgtaaa ggagtttatt cccagattat ctcttgaggt
     9961 tccctcactg gagaatggtt taattaaaga tgatggtaaa acttacgttt ttccccttcg
    10021 agaaggggta aagttccaca acggtagcga cctcaccccc gaagatgtgg aatactcttt
    10081 tgagcgaggg attctcaccg acccttatgc cggacccatg tggatgatta tcgaggctct
    10141 gtttgattat caaaccatag aagatatggt aggggataag ctgcaaatag cctggagcga
    10201 tatggttaac gaggacggga ccttaaaaag cgaagaatac gcttctaaac ttgtcgattt
    10261 ttaccaccag tatattgacc cagctattga agtagaaggg caaaatgtgg tgtttcattt
    10321 agtgcgacct tttgctcctt ttttgagtat cctatctcaa aactcaagtt ggagcgccat
    10381 tatggataaa gagacctgtg tttctctggg tctgtggaat ggagagcccg atggctggtg
    10441 gaaatatcat accctgaaaa aagaagaaag tcctctttat gagaaagcca taggaacagg
    10501 cccttttgtg ctggtggaat gggatagagc gcaacaaaag ttgactttta atagaaatga
    10561 taactactgg ggagaaaagc ctaaaattgc taaagcggta atctggggaa ttgatgagtg
    10621 gagcacccgc cgggcaatgt tagaagccgg agatgctgac cagatatata ctccccttca
    10681 atatttagaa caggtaaaag gaatgccggg agtgacaatt gaagaggaag cgcggattac
    10741 cattactgct ttacacttta actggacggt ggaaaaagga agccaatact taggaagcgg
    10801 tcaattagat ggagaaggaa ttcctcctga tttctttagc gatgtccata tccgtcgtgc
    10861 tttctcttat gcttttgatt atgatacttt tatagagcaa gtgctccaag gttatggatt
    10921 tcggattcct tcggtaattc ctaaggggct tttaggattt aatgaaggcc ttcctatgta
    10981 taattttgac ttagaaaagg caaaagaaga atttcagcag gcctggaatg gagaagtatg
    11041 ggaaaaagga ttcaagctta ctctccttta caataccgga aatgaagctc gacagactgc
    11101 ctgtgagatg ctgaaagaat acatcgaatc tctcaatcct aagtttaaaa ttgaagtgca
    11161 aggtgttcag tggcccactt acctggatgc ttaccggagc ggtcagcttc cagcttttat
    11221 aattggctgg ttagctgatt atcctgaccc tcacaatttt gtctttactt acttccatag
    11281 taatggagtt tatggcacca ctcaagggca ggcttttctg gattttgctc gagaaaattt
    11341 ggattcactg gtagagaaag ctatagctga ggtagatgat gaggcacggc aaaaaattta
    11401 tgaagagatt cagaccattg cttatgaaaa cgctctgggt atgactctct atcaacccca
    11461 agaaatttat cctatgagag attgggtaaa aggatggacg tttaatccta tgagaccagg
    11521 agaggttaac tgggaaggag tatataaaga agagtgaatg aaaggaaaaa agaaggggag
    11581 agaaatctcc ccttcttttt ataaggtgaa aagaggatga ttttttacat tattcgtcgg
    11641 cttttgtttc ttcccttgac tttactgggt atttcaattc ttgtttttct catgattgga
    11701 ttattgagcc cttatcagag attagctact tttattaata gtccggagag actaaaaggg
    11761 gcagacattg ataaattagt agaattttat ggtctgaatg atcctctcgt cgtccaatat
    11821 ggaagatggc taaaaaacct ctttcagggt aatttgggct ggtcttacgt agggaaagct
    11881 ccagtgtatg atgcacttgt ggagcggttt ccttatactc tagagctggc tttattttcc
    11941 atctttccta tcatttttct gggaatacaa ttaggagtga tatcggcggt aaaccacaat
    12001 cgtcccttag accatggttt acgtatattt gcagtagtgg gatggtcttt tcctgatttt
    12061 gtttttggat tatttatttt gatgattttc tatagtgtat tgggctggtt ccctccgggg
    12121 aatctgagcc tgtgggcaga agaagtggtt aaatcttctg cttttcataa ttttaccggc
    12181 atgaccacta tagatgctct tctcaatgga agactggata ttttctggga tgctt
//
LOCUS       DAOSVE010000048        12131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_3032_length_12131_cov_69.229795, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000048 DAOSVE010000000
VERSION     DAOSVE010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 12131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12131
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3032_length_12131_cov_69.229795"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            714..2282
                     /locus_tag="PK016_05325"
     CDS             714..2282
                     /locus_tag="PK016_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HXL02523.1"
                     /translation="MANGYRVGVVDNLDKLFAVLPDHVRIPLEERENADDLIEVVMDL
                     GRPPEARFARGFVVLSDRIIEREDLDYVVQRVGAFTRDNRAGIERTLHRISCIRNRLG
                     NIVGLTLRVGRAVYGTIDIIRDVIVSGKNILLLGPPGVGKTTKLREVARVLSDEFQKR
                     VVVVDTSNEIAGDGDIPHPAIGRSRRMQVSSPEKQHDVMIEAVENHMPEVIIVDEIGR
                     EEEARACRTIAERGVQLIATAHGNTLKNLLLNPTLSDLVGGIQSVILSDEEAHRRGSQ
                     KAILERKAIPTFDVVIELRDRDTLGIYHDTAQAVDLLLRGYDPRPEVRKRTKEGAVKV
                     VEEVKSASPLAGVNMAKEEEATPELFESQLLRVYLFAVSKNYVQRAIAHAKVPLEVVE
                     DVNEADILLTLKSRYRNKGSKIREAENRGIPVHVVRSNTYNQIYKFVQDLFGYSDLSS
                     GNGESGLIEAERAARQVLKAGEAIELSPRSSSVRRLQHKIAERYHLFSKSIGEEPFRR
                     VVIYPRILEENRHE"
     gene            2275..2931
                     /gene="tmk"
                     /locus_tag="PK016_05330"
     CDS             2275..2931
                     /gene="tmk"
                     /locus_tag="PK016_05330"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HXL02524.1"
                     /translation="MSEKYGFLVTLEGIEGSGKSTHARRLFSFLKEAGIPCLLTREPG
                     GTPMGEKIRDLLLNPESGKVDPLTETFLFEASRREHVKEVINPALRGGKVVICVRFTD
                     STLAYQGYGRKVPLPYLYYLNNIATDGIVPDLTMLFDVEPEAGLARSFSHTEERELRF
                     EKEFLSCKEFLFAVRNGYLQLAKESPHRFVVVDTTDSSKEEVFSIIQNTVWQRLKGRG
                     "
     gene            2936..3274
                     /locus_tag="PK016_05335"
     CDS             2936..3274
                     /locus_tag="PK016_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HXL02525.1"
                     /translation="MSPRLLLICVVRDQDVAKLLDNLRDRNISFTKLASTGGFLREGN
                     TTLLIGIEEERREEVRKLIRETCARREEIVESTVPLNEPIGPFVPQRIKVVRGGGVLF
                     EVPILYYEKF"
     gene            3310..4308
                     /gene="holB"
                     /locus_tag="PK016_05340"
     CDS             3310..4308
                     /gene="holB"
                     /locus_tag="PK016_05340"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HXL02526.1"
                     /translation="MSWSEICGHERQKEFFERVVLSGRIAHAYLFSGPPRVGKTAFAL
                     ELTKLLHCLNPARNGSCGQCKNCQLITNLTHPDVSLLSPLGDCLRIDEIRSFVRWLNF
                     KPIMGKMKVGIIEEAEKLNEEAANCLLKTLEEPPKDTVVILITSHRASLLPTIISRCQ
                     IIEFSLLSPLEISNYLIERKGWKEEEAKRVSSLSQGSLGRAEEIKESRDDLEDKFREL
                     WHKLKNETNSLSLGSWFYNNKEQLPLIMTVLEQYLRDLWIWKMTDGQCANLLFFERER
                     VQKEAKEQETETWRRLIILVDNLVYDISGNLNWDIVLSRFIFLWRGEWSHASGSGN"
     gene            4286..5077
                     /gene="ricT"
                     /locus_tag="PK016_05345"
     CDS             4286..5077
                     /gene="ricT"
                     /locus_tag="PK016_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HXL02527.1"
                     /translation="MPQVAGIKFENNPKIYHFETRGLDLHLGDKCVVETSIGLEIGIV
                     AKGVFLAPEDNFKPVIRKAQKIDLDQAEANREREKVAFQKAQELVAHYQIPMKILRVH
                     YTLDRGKLTFYFGSEERIDFRQLVKELAAIFHTRIELRQVGVRDEARMVGGCGMCGKE
                     LCCNAFLTNFDPISIKMAKEQNLVLNSAKISGVCGRLMCCLAFEYPLYRRLTHKLPKK
                     GSKIITPWGLAKILEVDIFKDRIFLELDDGKKIRINEEEYHRYFL"
     gene            5135..5740
                     /locus_tag="PK016_05350"
     CDS             5135..5740
                     /locus_tag="PK016_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXL02528.1"
                     /translation="MDKAEVQVWYQGLTLEEEKLWFEERVKSCERGLLRFAYHLSGNM
                     DKARDLVQEALLRAYQYRHSYNASYSFESWMSTILLNVHRQRFKKEKFFKIFRPGKER
                     EDGEETEVWDFLESKEEGPEERTLKREVLGFLREAIEKLPPQMKEVIVLCDVMNYSYE
                     EASEIIGCPLGTVRSRLHRARRKVKNLVEESYGEDFLSVWG"
     gene            5745..6197
                     /locus_tag="PK016_05355"
     CDS             5745..6197
                     /locus_tag="PK016_05355"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HXL02529.1"
                     /translation="MNCEEVERMLSAYLDEELVAEEYKSLKKHLMRCSRCQKELNKLR
                     AIKSLMRDLDNGFNFNQDFKFDWEAVEKKARTIKKREAILFSLAAALIVMVLFFVVFQ
                     EQAQWETEVMHPEILVNLHESLSGNQLDIPFKQETNFIGTLELVSGGR"
     gene            6197..7171
                     /locus_tag="PK016_05360"
     CDS             6197..7171
                     /locus_tag="PK016_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02530.1"
                     /translation="MRKSGFSFFIEFSSSFLIFCLCSSFLSIARAEIVNPEEALYFLE
                     EMIKKSTDRDYWGIWSNQDYSSGKKDFYEVVHLRYIGLAWRNLNEGDLVTVKKGNYRY
                     VVDLVSGEVKGVYPLYDLPFLPPEEDGLELLLENYLLDIQDREGQVISRHTGKVVRSF
                     SVDEKGLLSSQAFYTPEGKLKEEGSFVYRDYSPDYTQLVKYVEAMKKPSDVTIPSLTV
                     SSKELFQPSLLPPGFHLKQVYLVKNQGRDWYQLVYTDGLQYFSLWQSVYPSTLSGDKR
                     NRKISLKKEDDIVTLMGEKGGFYLALMGNFDLEMITQIFNSITWEGGN"
     gene            7174..8583
                     /locus_tag="PK016_05365"
     CDS             7174..8583
                     /locus_tag="PK016_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HXL02531.1"
                     /translation="MWFKKTKLLSFWTVVLLVSLMWGVAGAVTSIPEDTNVIADIVEK
                     VTPAIVNIDTLKMATYSSPFAPFLNDPFFRKFFEGMIPEERQVPVKGLGTGFVFRSDG
                     YILTNAHVVSGADEIKVTFLDGREFEGKVVGSDTLTDIAVVKIDAQGLPTIPLGDSDT
                     ARVGEWVVAIGNPYGLSHTVTVGVLSAKGRPIAAGDSGQEYENFLQTDAAINPGNSGG
                     PLINLNGEAIGINTAILPYAQGIGFAIPINMAKSILDELITEGKVVRAWLGVVIQNLT
                     PEIAEKFGLEEAKGALVADISPDSPAEKAGIKRGDVILKVDEVETPDISTLQQTIRSH
                     RPGDVVNVEIWRDGKTITLKVTLAELEESTSVLPVPEKIDLGMEVEEITSDLADRYAL
                     REKSGVVIVGIKAGGPADDAGLRPGDVILEVNRKEIKNLDDWKAAINEIEPEETILLL
                     ISRGGQTYFVPLKAEEPES"
     gene            8589..9437
                     /gene="rsmI"
                     /locus_tag="PK016_05370"
     CDS             8589..9437
                     /gene="rsmI"
                     /locus_tag="PK016_05370"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HXL02532.1"
                     /translation="METVKEWGKLYFCPTPLGNLQDITLRTLQVFREVDFIACEDTRV
                     TLKILNHYHIQKPLFSYHSYNLKEVSSRIVNLLKGGKNIALVSDAGMPGIQDPGMELV
                     NILEKEGLSFEVLPGPSALVVAVVYSGFAFSGFTFLGFLPRKKKERRGILERLLHLPQ
                     AMVIYESPHRVVSTLEEIKEMVGSERRVIFLRELTKIHEEILRGKLDEISEILSQREE
                     IKGEVVLVIEGEKREKQIPLEAEKLIKALSQQGIAPRQILEVVSNTFSISKNRLKEVW
                     EKEKLS"
     gene            9540..10382
                     /gene="pstC"
                     /locus_tag="PK016_05375"
     CDS             9540..10382
                     /gene="pstC"
                     /locus_tag="PK016_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005995637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HXL02533.1"
                     /translation="MLFLVAFSSLFFLIGIVVVLFVEGLPVFTHVSLSQFLLGRRWYP
                     TSSPPQFGALPLILGSLLVTGGGLLFAVPLGLFAGIFLAELCPRRGREILKPVVELLG
                     DIPSVVYGFFGVVVVVPFIRDLLSLPTGLTAFTAALILGIMAVPSIVSVIEDTLTSVP
                     RSYKEASFALGATHWETLHRAILPAASSGIGAAIILGAGRIIGETMAVLMVAGGAPVI
                     PKSFFQPVRTLTATIAVEMGEASVGSPHYHSLFGLAIILFLFTLALNILVENLIPGGR
                     RRIR"
     gene            10379..11254
                     /gene="pstA"
                     /locus_tag="PK016_05380"
     CDS             10379..11254
                     /gene="pstA"
                     /locus_tag="PK016_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HXL02534.1"
                     /translation="MMKEESLKKRYLVQNIWFTIFWLSMILITVILGLILYFILARGI
                     KVINWEFLIAAPRRGMTEGGIFPAIVGTFYLVLGSIVVSLPLGVFSAVYLTEYASSGS
                     PAVKAIRTAIHCLAGVPSVVFGLFGLAVFVKILGFGVSVLSGSLTLGIMSLPVVITSV
                     EEALKAVPLSFREASLALGANKWTTILRVVLPSSLPGILTGLILSVGRVAGETAPIMF
                     TAAVFYARRLPQSLFDPVMSLPYHIYGLMTEGTRPDKQIPLAYGTAVVLILLVMAVDS
                     IAIYLRGKSRRARKW"
     gene            11258..12016
                     /gene="pstB"
                     /locus_tag="PK016_05385"
     CDS             11258..12016
                     /gene="pstB"
                     /locus_tag="PK016_05385"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HXL02535.1"
                     /translation="MEEVKLQIKDLSVFFGEKQVLHQINLNIPANKVTAIIGPSGCGK
                     STLLRSINRMNDFFEGVRVEGEVIFDGGNIYGESVDPVMVRRRIGMVFQMPNPFPKSI
                     FENVAYGLRIQGWKNKKAIEEQVVKSLQAAALWDEVKDRLHSSAFDLSGGQQQRLCIA
                     RAIAVEPEILLMDEPASALDPIATARVEELIRSLIEKYTIVLVTHNMQEAARTSDYTA
                     FLLLGRLIEFGLTNQIFTRPQKKETEDYLTGRFG"
BASE COUNT         3449 a         1958 c         2996 g         3728 t
ORIGIN      
        1 cgagggcgag ggaaaaaaga tgtaaagctc tcccttcgag ggcgagggaa ggaatgtgtg
       61 gggttcttcc attgaaggcg agggaagaaa aagaaagggg attcataagg gggaattctt
      121 tcccccttat actgagaaag acgcttttcc ttcctcagta gtttttgtct tttcagtttt
      181 taattttttg ctttggggga ttataaaggg gcacagcccc tttatagttc gggattataa
      241 aggggtgctc ccctttaagg gattttttat ccctcagtaa tttctgtttt tttctgtttt
      301 tcttttaatt ttttgaaggg ggttatgagg gggaaaatat ccccctcata gtcattataa
      361 ttagctttgc ggttaactat atattatcct ctgattcttg caaaggtgga aaagacaaag
      421 acgataagag acttaccccc tctactgagg aagaaaaaag tatctttccc agtagagttc
      481 tgaaagtgag tataaagtgt tatgtagaaa agtcgctata ccacttaaag aagctccaga
      541 gctgagataa gagataaaat ttttctttgt ctctacaagc tgttataatt aggtatattt
      601 gtatttggtg actttactaa gaagggcttt tgttgaaata aacaaagtgt atgctggttt
      661 ttgataattg aagataaaag caaggtagaa ggaggataga aaagaacagt actatggcta
      721 atggttatcg agtaggagta gtagataact tggataaact ttttgcagtt ttacctgatc
      781 atgtcagaat tcctctggaa gagcgggaga acgctgatga tttaatagag gtggtaatgg
      841 atctggggcg tcctcctgag gctcgctttg ccaggggttt tgtggttctt tctgatagaa
      901 taatagaaag agaagacctt gattatgtgg tacagagagt gggggcattt actcgagata
      961 accgagcagg tatcgagcgg actcttcacc gtatttcctg tattcgtaac cgattaggaa
     1021 atattgtagg acttactctt cgagtgggaa gagcagttta tggtactatt gacataatta
     1081 gggatgttat agtatcaggt aaaaatattt tacttttagg ccccccggga gtgggtaaaa
     1141 ctactaaact tcgggaagta gcccgggtgc tgagtgacga atttcaaaaa agggtagtag
     1201 tggtggatac ctctaatgag atagctggtg atggagatat acctcatcct gccattggcc
     1261 ggtctcgtcg gatgcaggta tcatctcctg aaaagcaaca tgatgtgatg attgaagcag
     1321 tagaaaatca tatgcctgag gttatcatcg tagatgaaat tgggagagaa gaagaagcaa
     1381 gagcctgccg tactatcgca gagagagggg ttcaactcat tgctacagct catggtaata
     1441 ctttgaagaa tctcctcttg aatcctactt taagcgatct ggtgggtgga attcagtcgg
     1501 taatattgag tgatgaagaa gctcatcgtc gggggagtca aaaagcgata ctggagcgca
     1561 aagctatccc tacttttgat gtagttattg aattgcgtga ccgggacacc ttaggcattt
     1621 accatgatac tgctcaggcg gtagatttat tgttgcgagg ctatgaccct cgaccggaag
     1681 taagaaaaag aaccaaagag ggggcggtta aggtggtgga agaggttaaa agtgcctccc
     1741 ctcttgcagg tgtaaatatg gcaaaagagg aagaagcgac tcctgaattg tttgagagtc
     1801 aactgttgcg ggtttaccta tttgctgtga gcaaaaatta tgttcagaga gctattgctc
     1861 atgccaaagt tcctcttgag gtagtggaag atgtaaacga agcagatatt cttcttactt
     1921 tgaaaagtcg ttatcgtaat aaaggaagta agataagaga agcagaaaac cggggtatac
     1981 cagttcatgt ggtgcgcagc aacacttata atcaaattta taagtttgta caggacctct
     2041 tcggttatag cgacctctct tcaggtaatg gagaatcagg attaatagaa gcagaaagag
     2101 ccgctcgaca ggtactcaaa gcaggagaag ccatagagct ttctccccgt agctcttcag
     2161 taaggcgttt gcagcataaa attgctgagc gctaccatct tttctctaag agcataggag
     2221 aggaaccctt ccgccgggtg gtgatctacc ctcgtatttt ggaagagaac cgccatgagt
     2281 gaaaaatacg gttttttggt cactttggaa ggaattgagg ggagcgggaa gagcactcat
     2341 gctcgtcgtt tattttcttt cctgaaagaa gctggtattc cctgtttgct aacccgagag
     2401 ccagggggga cacctatggg ggaaaaaata agagacctgt tgttaaatcc agaaagtggt
     2461 aaagttgacc cgcttactga gacttttctt tttgaagctt cacggcggga gcatgtaaaa
     2521 gaagtgataa atcctgctct gcgaggaggt aaagtagtaa tatgtgtccg atttactgat
     2581 tctaccctgg cttatcaggg ctacggtcgt aaagtcccgc ttccttatct ttactatttg
     2641 aataacattg ctactgatgg cattgtacct gatttaacca tgctttttga tgtagaacca
     2701 gaagcggggt tggctcgctc tttttctcat actgaagaaa gagagctacg ttttgaaaaa
     2761 gagtttttgt cttgtaagga atttctcttt gctgttcgga atggctatct tcaactggct
     2821 aaagaatctc cccaccgttt tgtggtggtg gatactactg attcttctaa agaagaggtt
     2881 ttttctatta ttcaaaatac tgtttggcaa agattgaagg gaagggggtg agtttatgtc
     2941 tcctcgttta cttttaatat gtgtggtgcg agaccaggac gttgctaagt tgctggataa
     3001 tctgagagac aggaatattt ctttcactaa attagcttct acaggtggct tcctgcggga
     3061 aggaaatact actcttttaa tcggtataga ggaagaacgc agagaagaag tgaggaaatt
     3121 aattagagaa acctgtgctc gtcgagaaga aattgtagaa tctaccgttc ctctcaatga
     3181 acctatcggt ccctttgttc ctcagaggat taaggtggtg agagggggag gggttctttt
     3241 tgaggttccc attttatact atgagaagtt ttgacctctt tttcggaaaa ataaaatgag
     3301 ttgggcttga tgagctggtc tgagatttgc ggtcatgaaa ggcagaaaga attttttgaa
     3361 cgggttgttc tttcaggaag aattgctcac gcctatcttt tttccggccc tccccgggtg
     3421 ggaaaaactg cctttgctct ggagttaact aaacttcttc attgtttgaa tcctgcccgg
     3481 aatggaagct gcggtcaatg taaaaactgc cagttgataa ctaaccttac tcatcctgat
     3541 gtttctcttc tttccccttt gggtgactgt ttaagaatag atgaaataag aagttttgtg
     3601 aggtggctta atttcaaacc gataatgggt aaaatgaaag tggggataat agaagaggca
     3661 gaaaagctaa atgaagaagc agctaattgt cttttaaaga ctctggagga accaccaaaa
     3721 gatactgtgg taattctgat tacttctcac cgggcttctc ttttacccac cataatttct
     3781 cgttgccaga taatagaatt ttctcttctt tctcctttag aaatcagcaa ttatttaata
     3841 gagagaaaag gatggaaaga agaagaagct aaaagagtat cttccctttc tcagggtagt
     3901 ttagggagag cagaagaaat caaggagagt agagatgatt tagaggataa gttccgggaa
     3961 ttgtggcaca aattaaaaaa tgaaactaat tctctctccc tgggttcctg gttttataat
     4021 aataaggagc agctacccct gattatgact gttttggagc agtatttgag agacttatgg
     4081 atatggaaga tgactgatgg tcaatgtgcc aatttgcttt tttttgaaag agagagggta
     4141 cagaaagaag cgaaagaaca ggaaacggaa acctggagga gattaataat cttggtagac
     4201 aatctagtct atgatatttc tggtaatctg aactgggaca tagttttaag tagatttatt
     4261 tttttatgga gaggtgaatg gagtcatgcc tcaggtagcg ggaattaaat tcgagaacaa
     4321 tcctaaaatt tatcattttg aaacccgggg tttagactta cacctgggag ataaatgtgt
     4381 agtagagact tctataggtt tagaaatagg catagtagca aagggagttt ttcttgctcc
     4441 cgaagataat tttaagccag taattcggaa agctcaaaag atagatttgg accaagctga
     4501 agctaatcgg gagagagaaa aagtagcttt tcaaaaagct caagaattgg ttgctcacta
     4561 ccagattccg atgaaaattc tgcgggttca ttatactctg gaccggggta agttaacttt
     4621 ttactttggt tcagaagaaa gaattgattt tcggcagctg gttaaagaat tagctgctat
     4681 ttttcacacc cggattgaat tacggcaggt gggggtgaga gatgaggccc ggatggtggg
     4741 tggatgtgga atgtgcggga aagaattatg ctgcaatgct tttttgacca attttgatcc
     4801 catatctatt aagatggcta aagaacagaa tttagttctt aattcggcta aaatttctgg
     4861 agtttgtggt cgccttatgt gttgtctggc ttttgaatat cctctgtatc gccggcttac
     4921 tcataaatta cctaaaaaag gtagtaagat aatcactcct tggggattgg caaaaatttt
     4981 agaagtagat atttttaaag ataggatttt cttagagctg gatgatggga aaaaaattag
     5041 aatcaatgaa gaagaatacc atcgttattt tttgtagatg agaaaggaac tttttaatgg
     5101 ctcgagaata tttgggagat agaaggtgag atgaatggat aaagctgaag ttcaagtttg
     5161 gtatcaggga ttaactctgg aagaagaaaa gttgtggttt gaagaaaggg ttaaaagttg
     5221 tgaaaggggg ctcctgcgtt ttgcctatca tctgagtggt aatatggata aagctcggga
     5281 tttggtacag gaagcccttt tgcgagctta ccagtatcgg catagttata atgctagcta
     5341 ttcttttgaa agctggatgt ctactatctt acttaatgtt catcgtcagc gttttaaaaa
     5401 agagaagttt ttcaaaatct ttcgtcctgg aaaagaacga gaagacggag aggaaactga
     5461 agtctgggat tttttagaat caaaagaaga aggcccagaa gagagaactt tgaaaagaga
     5521 agttcttggt tttcttcgag aagctatcga aaaacttccc cctcagatga aggaagtaat
     5581 agtgctctgt gatgtgatga actactctta tgaggaagcc agcgaaataa ttggttgtcc
     5641 cctggggaca gttcgttccc ggcttcatag agctcgaaga aaagtgaaaa atttagtgga
     5701 agagagctat ggagaagatt ttctctccgt ttgggggtag ggatatgaat tgtgaagaag
     5761 tagagagaat gctttctgct tacctggatg aagagctggt ggctgaagaa tataaatctt
     5821 tgaaaaaaca tctaatgcga tgttctcgtt gtcagaaaga gttgaataaa ctccgggcaa
     5881 ttaagagttt gatgagggat ctggataatg gttttaattt taaccaggat tttaaatttg
     5941 attgggaagc ggtagagaaa aaggcaagga caataaagaa gagagaagca attctctttt
     6001 cccttgctgc tgctttaata gttatggttt tgtttttcgt tgtttttcag gagcaggctc
     6061 agtgggaaac ggaagttatg catcccgaga ttttagtaaa ccttcacgag agcttgagcg
     6121 gcaaccaatt agatatacct tttaagcagg aaaccaattt tattggaact ctggagctgg
     6181 tctccggtgg tagatagtga gaaaatcggg gttttcgttt tttatagaat ttagttcttc
     6241 ttttctgatt ttttgcctat gttcttcatt tttatccatc gctcgagcag aaatagttaa
     6301 tccagaagag gctttgtatt ttttggagga aatgatcaaa aaatctactg accgtgatta
     6361 ttggggaata tggtctaatc aggactattc ctcaggaaaa aaggactttt atgaagtggt
     6421 tcacttgaga tatataggtt tagcgtggag gaacttaaac gagggagacc tggttacggt
     6481 taaaaaagga aactatcgtt acgtagtgga cctggtaagc ggagaagtta aaggagttta
     6541 tcctctttat gacctccctt ttcttcctcc tgaagaggat ggtttagagt tgcttttaga
     6601 aaattattta ttagatatac aagatagaga aggacaagtg atttctcgcc acaccggtaa
     6661 agtagtgcgt tccttttcag tagatgaaaa aggtttactg agctctcagg ctttttacac
     6721 tccagagggg aagctgaaag aggagggaag ttttgtgtat cgggattatt ctcccgatta
     6781 tacccagtta gtaaaatatg tagaggctat gaaaaaacct tctgatgtta ccataccctc
     6841 tcttactgtt agtagtaagg agttattcca gccttctcta ttgccccctg gttttcatct
     6901 gaaacaggtt tatttagtga agaatcaagg tcgagactgg tatcaactgg tttatactga
     6961 tggtctgcag tatttttctc tgtggcagag tgtttacccc tcgacgttat ccggggataa
     7021 gagaaacagg aaaatctctt tgaaaaagga ggatgatata gttacgctga tgggagaaaa
     7081 aggcggattc tatttagctt tgatgggaaa ttttgattta gaaatgataa cgcagatttt
     7141 caattctatt acctgggaag gaggtaatta gctgtgtggt ttaaaaaaac caagctttta
     7201 agtttctgga cagtagtttt gctggtttct ttaatgtggg gagtagctgg agcagtaact
     7261 tccatacctg aagataccaa cgtcatagct gatattgtag aaaaagttac tccggcgata
     7321 gttaacatag atactctgaa gatggccact tatagctctc cctttgctcc ttttttaaat
     7381 gaccccttct tccggaaatt ttttgaaggg atgataccgg aggagaggca ggttccggtt
     7441 aagggattag gcactggatt tgtatttcgt tccgatggct atattttgac taatgcacac
     7501 gtggtgagtg gagcagatga aataaaagtt acttttttag atggtagaga gtttgagggt
     7561 aaggttgttg gttccgatac tctcaccgat atagcagtgg tgaaaattga tgctcaaggt
     7621 cttcctacta ttcctttggg tgactccgat actgcccggg tgggagaatg ggtagtggcc
     7681 attggtaatc cttatgggct ttctcatacg gttactgtgg gggttttgag tgctaaagga
     7741 aggcctatcg ccgctggaga ttcgggtcag gagtacgaga atttcttaca gactgatgcg
     7801 gctatcaatc cgggaaacag tggaggtcct cttattaatt taaatggaga agctattgga
     7861 ataaataccg ctatccttcc ttatgctcag ggtattggat ttgctattcc tataaacatg
     7921 gccaagtcta tccttgatga gcttataacc gaaggaaaag tagtaagggc ctggttagga
     7981 gtggttattc aaaaccttac tcctgaaata gccgagaaat ttggtttgga agaggccaaa
     8041 ggggcgttgg tagcagatat ttctcctgat agcccagcgg aaaaagccgg tattaaaaga
     8101 ggcgatgtta ttttaaaggt ggacgaggta gaaactcctg atataagtac tctgcaacaa
     8161 actattcgct ctcatcgtcc tggagatgtg gtgaatgttg aaatctggag agatgggaaa
     8221 accatcactt tgaaagtaac tttagcagaa ttagaggaga gtacttctgt attaccagtt
     8281 cctgagaaaa tagatttagg tatggaagta gaagagatta cttctgacct tgctgataga
     8341 tatgctctgc gagagaaaag tggagtagtg atagtgggaa taaaggctgg tggtcctgct
     8401 gatgatgctg gcctgcgtcc cggggatgta attctggaag ttaaccggaa ggaaatcaaa
     8461 aacttggatg attggaaagc tgctataaat gaaatagagc cagaagaaac tatcctttta
     8521 cttatcagca ggggaggaca gacttatttt gttcctttaa aagctgaaga accagagtct
     8581 taaaatttat ggaaacggta aaggagtggg gcaaacttta cttttgcccc actcctttgg
     8641 gtaatttgca ggatattact ctgcgcactc tccaggtatt tagagaagtt gatttcattg
     8701 cctgtgaaga cacccgagtt actttgaaaa ttctcaacca ctaccatatt caaaaacctc
     8761 tttttagcta tcactcctat aatttaaaag aagttagctc tcgaatagta aacttactta
     8821 aaggaggtaa aaacatagct ttagtttctg acgctggaat gccgggaatt caagatccag
     8881 ggatggaatt agtgaatatt ctggaaaaag agggtttaag ctttgaagtt ctgccaggtc
     8941 cttctgcttt agtggtagca gtggtttatt caggattcgc tttctcgggt tttacttttt
     9001 tgggattttt accccggaag aaaaaagaga gaagaggaat tttagagcga cttcttcatc
     9061 ttccccaagc aatggtgatt tacgaatctc ctcatcgggt ggtatctact ttggaagaaa
     9121 ttaaggaaat ggttggaagt gaaagaagag ttattttttt gcgggaattg actaaaattc
     9181 acgaggaaat attgagaggg aaactcgacg agatttcgga gattttatct cagcgagaag
     9241 aaataaaagg agaagttgta ctggtaatag agggggaaaa gagggaaaaa cagatacccc
     9301 tcgaggcaga aaagttgatt aaggctctct cccagcaggg aattgctccc cggcaaatat
     9361 tggaagtagt gagtaatacg ttttcgataa gtaaaaatag gttgaaggag gtttgggaaa
     9421 aagaaaagtt gagttgattt tgaagggaaa aactgcttta attaccaatg tagaaatttt
     9481 accccctgag tgaggaggta gtagttctga aaagaaaaaa gcgagaagga gcggaaataa
     9541 tacttttttt agtggctttt tcttctcttt tttttctgat tggtatagtg gtggttctct
     9601 ttgtggaagg attgccagtt tttactcatg tttctctttc tcagtttttg ttaggtagaa
     9661 gatggtatcc tacttcttct cctcctcaat ttggagcatt gcctcttatt ttagggtctc
     9721 ttctggttac tggtggaggg ttactctttg ccgttcctct ggggcttttt gctggtatat
     9781 ttctggctga gctctgtcct cgtcggggga gggagatttt gaaaccagtt gttgaactat
     9841 taggtgatat tccctctgtt gtctatggtt tctttggagt ggtggttgtt gtccctttta
     9901 ttcgcgatct tctctctcta cctactggtc ttactgcctt tactgctgct ttaattttgg
     9961 gcataatggc cgtgccttct atagttagcg tgatagagga tactcttacc tcagttcctc
    10021 gcagttataa agaagcgtct tttgctttgg gagccacaca ctgggaaact ttacaccgag
    10081 cgattttgcc tgctgcttct tcgggaattg gagcggcaat cattttaggg gcagggagaa
    10141 ttataggaga gactatggca gtattaatgg tagcaggagg ggccccggta atacctaaga
    10201 gctttttcca accggtacgt actttaactg ctactattgc tgtggagatg ggtgaggcct
    10261 cagttggtag cccgcactat cattctcttt ttggtctggc tatcattctc tttcttttta
    10321 ctcttgctct taatattctg gtggaaaatt taatccccgg aggaagaaga agaattagat
    10381 gatgaaagaa gagagtttga aaaaaagata tttagttcag aatatctggt ttactatatt
    10441 ctggttatct atgattttga ttactgtaat tctgggttta attttatatt tcattttagc
    10501 tcgagggata aaggtaatca actgggaatt tttgattgct gcccctcgac ggggtatgac
    10561 cgaaggtggt attttccctg ctattgtggg gactttttat ctggtattgg gtagcattgt
    10621 ggtatctctt cctttaggag ttttttctgc tgtttatcta actgaatatg cttcttcagg
    10681 tagtccggca gtaaaagcca ttcgtactgc tattcattgc ctggcgggag taccttcagt
    10741 ggtttttggt ttatttggtc tggcggtttt tgtcaaaatt ctggggtttg gtgtttctgt
    10801 tctatctggt tctttaactc tgggtattat gtctcttcca gtggttataa cctcagttga
    10861 agaagctcta aaggcagttc ctctttcttt tagagaagca tcgttagctc tgggagccaa
    10921 caaatggact accatcttgc gggtagtttt acccagctct ttaccgggca ttcttactgg
    10981 cttaattctt tctgtgggta gagtggcagg agaaactgct cccatcatgt ttactgctgc
    11041 agtattttat gctcgaagat tgcctcaatc tctttttgac ccggttatgt ctcttcctta
    11101 tcacatttat ggtttgatga ctgaaggaac aaggccggat aaacagattc ctctcgctta
    11161 tggaacagcg gtggttttga ttttgctagt tatggctgtt gattctatcg ctatttattt
    11221 acggggaaaa tccaggcgag ctagaaagtg gtgaaaaatg gaagaagtta aactgcaaat
    11281 taaagatttg agcgtgtttt ttggagagaa acaggtactc caccagatta atttaaatat
    11341 tccagcgaac aaagttactg ctattattgg cccttcaggt tgcggtaaat ctactttgct
    11401 gcgaagtatc aatcgaatga acgatttttt tgaaggtgtg agagtagaag gcgaggttat
    11461 ttttgacgga ggtaatattt acggggaaag tgtagatccg gtgatggtaa gaagacgcat
    11521 tggtatggtt tttcaaatgc ccaacccctt tcctaaaagt attttcgaaa atgtagctta
    11581 cggtttgcga attcaggggt ggaaaaataa gaaagccatt gaggaacagg tggtgaaaag
    11641 cctgcaggca gcagccttat gggatgaggt aaaagacagg ctgcactctt cggcttttga
    11701 cctttctgga ggtcagcaac aacgtctctg cattgctcga gctatagcag tggagccaga
    11761 aatcttgctc atggatgaac cagcttcagc gttagaccct attgctactg ctagggtaga
    11821 agagttaatc aggagtttaa tcgagaaata taccattgtt ttggtaactc ataatatgca
    11881 agaagcagct cgtacttcag actatactgc ctttctactt ttaggaaggc tcatagaatt
    11941 tggtctcacc aatcaaatct tcaccaggcc tcagaagaag gaaaccgaag attaccttac
    12001 cggaagattt ggctgatagg gaggaactga aagaagcaaa gggagataac ataagggtat
    12061 ggttttctcg ggttaaggag gttctctaac tatgcaaatt ttttctctga taattaaagg
    12121 tggatacgtt a
//
LOCUS       DAOSVE010000049        12068 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_3044_length_12068_cov_70.542329, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000049 DAOSVE010000000
VERSION     DAOSVE010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 12068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12068
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3044_length_12068_cov_70.542329"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PK016_05390"
     CDS             <1..408
                     /locus_tag="PK016_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL02536.1"
                     /translation="AMVENKTGSIINISSMAAFRPLTKTIAYSAAKAAITNFTQWLAV
                     HLNQNYSKNLRVNAIAPGFLITEQNRYLLIDEKTGEETERGRRIKDHTPMGRYGEPQE
                     LVGAIIWLCSEAASFVNGVVIPIDGGFSAYSGV"
     gene            589..1224
                     /locus_tag="PK016_05395"
     CDS             589..1224
                     /locus_tag="PK016_05395"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXL02537.1"
                     /translation="MKLVKDSISEQIYSIIKEEILLAKRKPGEQISPRKLADEFETSV
                     MPIRDALNQLANEGLLVRKPRVGFFVRSFTQQEAQDLMEARKLYELYCLEKYFDRIDR
                     KKLSDYLNRSLERIDISYKDREFDELDENIHDLIVKASHNSWLINGYNSIRDYIIVLK
                     HLDIGRVQMAQKEHISLIEAILNGDKEKAKKTLEEHIQRVTEELTMNLSPR"
     assembly_gap    1286..1385
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1680..2321)
                     /locus_tag="PK016_05400"
     CDS             complement(1680..2321)
                     /locus_tag="PK016_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXL02538.1"
                     /translation="MKILVISDRINPLIYGDFCQERFKDVECIISCGDLPEHYLDFIV
                     TNLNIPLFFVHGNHDPSGGKKILAGGINLDERVITYRGVIIAGLEGSYWYNGGIHQYT
                     EREMYFKYLRLLPSLWWCKFFQGRFLDILVTHAPPQGVHEGNDLAHRGFSTFTHIIKK
                     YQPRYHLHGHVHIYDHNQNREDQLFNTQVINCYNYRIIEINSSELSSKTKVAL"
     gene            complement(2379..4895)
                     /locus_tag="PK016_05405"
     CDS             complement(2379..4895)
                     /locus_tag="PK016_05405"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXL02539.1"
                     /translation="MAKLRTRLFFRAFLIASLATIDFLPALGVPIPPLGFIPIFSFVL
                     LGAPAIWYYHLVDITPALAAQEIINNMSDALLVFDREGIVQLVNPASCQLIGKKEREI
                     KGLPIRSLLPHLFSPEEALQEDSRINHSFTLYPESEKELYLEASLSPIQRAGEILAWV
                     ALIRDVTELKKAQRELSQSEERYRTIFENTGSATILVEEDMTISLANREFEKLTGFPK
                     EEIEGKRKFTQFFAPPDVSQMIEYHQKRRVDPEIVPKKYTTHLIDQSGNVREVLASVD
                     LIPHTKQSVISLVDVTDLKRAEQQIAFQLDMLSHLYQGAQELSGTLDLGKLAQLAVYY
                     SAQEFKVKVASFLILKDNQKWETISTFPSSYSPILQSWLDSPEKDQFLADLLHSGSSL
                     VVNFTEETKYPQLEKIAKELEIKEVEAFLLGKTPDSWGALVWLVDKPDFLSSLSGFIQ
                     TFIHQVVTSGEQSRLFSQLQRYINMMRSLHSIDRAIASSLDLQMVLDFTLQEALRHLK
                     IDAMGIYLIDSSVLRYKAGLGFLFPEAASYQFRLEEGALGKAVLEKKPLFISHIEEEE
                     KKVLPPFIERENFLSLFAVPLVAKGETEGVLIAFHRLPFNPPPDWTEFLITIGGQLAI
                     AINNIAMFEELNRSHIELTLAYDTTIEGWARALELRDRETEGHTRRVAELTLKLAQTL
                     GVSPENLIHIRRGALLHDIGKMAIPDRILLKPGPLTAEEWEIMKKHPVYAWELLSPIK
                     YLHPALDIPYYHHERWDGTGYPRGLKGEEIPLSARIFAVIDVWDALISDRPYRSAWPQ
                     EKALKYIKKQSGHHFDPRVVEKFLELLEELPKSKKGAGYF"
     gene            5137..5394
                     /locus_tag="PK016_05410"
     CDS             5137..5394
                     /locus_tag="PK016_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02540.1"
                     /translation="MENWKRAVEIKGTPKKANLDHTSAVSSSGDTYIKELGNSHRKIE
                     KVIKKKRMLSREAFSRIKILIPVERIVRLVKRTARAKIVKL"
     gene            complement(5616..>7598)
                     /locus_tag="PK016_05415"
     CDS             complement(5616..>7598)
                     /locus_tag="PK016_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXL02541.1"
                     /translation="SRWLATGDPDGRITVSISSYFDSFSNDISTSSMISDQTKEDIIN
                     KLALYQQSWKNLQNNFSIATPEERDNLYMECHRSYSSLLRTLEDLRMDDWLDKLNIIN
                     QERKNYLLLSNSEAKESSRKKVETNMQQMKKFMEEDFLKIYGSVTAEAVINDRWRPFV
                     ELWNKVTAADDYLKFADEQTKKAITEASKTGQQVLAAMNEMNQEVLNQFDTTVTHVSE
                     LQNQLSRILYLVVGIIVILAIILGIVISRGIVSPINRSVSFAQVLEKGDLTQEISLRR
                     RDELGTLFNSLNQASRSLRAFLKEVAEGAEKIISSMEDLQHASREIAQTGNQIGQTIS
                     QVARGSEEQSQNLTEVSQNMEDLVGQVKGMAEQLKKQSDKASITLEAVDQVMESIQLT
                     GGNLEKVQESATQAFSLSEEGQKTLKEVVEAMGSISHSVLSVGEIVKTLGQSSREIGS
                     ITDLITGIADETNLLALNAAIEAARAGEAGRGFAVVADEVRKLAEESAHAAQRIAGLI
                     GDIQKEANKAVSSMEESTKRVDSGTKSVDRARQAFQDIYQANQVVTEETNAISTSFLR
                     VEEISQKIVQLVREMSEISHQNEIETQEVVGKAEEVFTRLSNVASISEENAAAAEEVA
                     ASAEEQSAALQEMDKEIQETMDMGRALQEDLEHFTI"
     assembly_gap    7599..7698
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7699..8015)
                     /locus_tag="PK016_05420"
     CDS             complement(<7699..8015)
                     /locus_tag="PK016_05420"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HXL02542.1"
                     /translation="MKIRTRIPLGFIVLALLAAVVGGFAIYAFREIGTSLEEVRNNTP
                     LLLATSKIKDLLSEKDSLVSSYLLEEDIATLDQMKEEFNQLDNKIMTYIEALRLGTES
                     EEF"
     gene            complement(8105..8854)
                     /locus_tag="PK016_05425"
     CDS             complement(8105..8854)
                     /locus_tag="PK016_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HXL02543.1"
                     /translation="MNPVLLGTLASTLAGLATGIGGLPVLFGRGQKISHQFKDELLGV
                     AAGIMLAATAFSLLVPAIEMGGAWITALGVAIGGVLLDVFDKIIPHEHFERGREGLES
                     DLRRIWLFVLAITLHNFPEGLAVGVSFGSGNISNGITVALAIGLQNIPEGLAVAVSLL
                     GVGYNQRQAFTYALLTGLVEPVGGLLGISLVTIMHSFLPLGMALAAGAMLFVISDEII
                     PETHHSSHARSSTYALLLGFIVMMILDTILG"
     gene            9221..10279
                     /locus_tag="PK016_05430"
     CDS             9221..10279
                     /locus_tag="PK016_05430"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HXL02544.1"
                     /translation="MKTYCIGVIPGDGTGPEVIREGLKVLEAISKKSHFSYTTIFYPF
                     GGEHYLKTGETLPDSAIEEFKKLDALYLGAIGHPEVKPGILERGILLKLRFSLDQYIN
                     LRPVKLYPGVWTPIKDKGPEDIDFVVVRENTEGLYVGAGGNLHRGTPHEVATQESINT
                     RRGVERCIRFAFEFTKKRNKKNKLTLCGKTNVLTYAFDLWERTFQEVAQEYPDIKTDY
                     AHVDATTMWMVKNPEWFDVIVTDNMFGDIITDLGAMIQGGMGIAAGGNINPEGVSMFE
                     PIGGSAPKYTGKNMINPLAAISALQMLLSHIGEEEAAQRVEKAIIKALESGKIRSMSA
                     GQMGLTTTEVGDLIASLV"
     gene            complement(10282..11235)
                     /locus_tag="PK016_05435"
     CDS             complement(10282..11235)
                     /locus_tag="PK016_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02545.1"
                     /translation="MKKEIFVSFFIFFLFFAGIVPLWAASDPLSLIEKSPYSEEDKEY
                     LRSQVDSLIKEAQEKSLPLSPIISKLKEGLSKKVLPHELVKTLNNKKGSLEKAQEILQ
                     KNGIKGEENLIIDLALSLELSVPPQAIEEALSQALANDGKRLQLIVDSLSSLLEMGVD
                     PEKAGEIVKAAADKNLSSRDMKKMAQLLENARKAGVDSEQVASVLANALEKHDNFNLV
                     EIEVQRFIASSKEKPALRSGQGVIVSSPGITSGGTPAEEGGTPLESTPPSAGKPPTQE
                     GGTPLEVAPSSEAKPSTEGATEKPSSSTSIPPVQEGGTPLD"
     gene            complement(11232..11744)
                     /locus_tag="PK016_05440"
     CDS             complement(11232..11744)
                     /locus_tag="PK016_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HXL02546.1"
                     /translation="MDIKEKKLVEYLENLPKVEVSEGFAQQVMYRIEGRKKKHLHYAD
                     YKKWVVVSLAAAFLVLIFFSNLPLLPELEISPSLGYRKIELVFYSPSQSSRTVAVAGD
                     FSSWDYLQMERVKEDCWRITLKVKPGKYQYGFLVDGKEWVADPNSLRQVPDGFGKFNS
                     VLVVNGESNS"
BASE COUNT         3558 a         2312 c         2405 g         3593 t
ORIGIN      
        1 gccatggtgg aaaataaaac tggctcgatt atcaacatat cttctatggc tgcctttaga
       61 cctttaacta aaacaattgc ttattctgca gccaaggcag ccattaccaa ctttacccag
      121 tggttggcag ttcatcttaa tcaaaactat tctaagaatc tgcgggttaa tgctatcgct
      181 cctggttttc ttattactga acaaaataga tatttattaa tcgatgaaaa aactggtgaa
      241 gaaacggaaa gggggcggag aattaaggac cataccccta tgggacgcta tggagagccg
      301 caagaattag taggggcaat tatctggctt tgttcagaag cggcctcttt tgtaaacgga
      361 gtggttattc ccatagatgg gggattttca gcttattcag gggtttaaga attcaggaaa
      421 agagcagctg tttgtttact taaccgctgt attttactat actggagtca gggatttagt
      481 cagaactgtt ctcgtctgct taaatccctt gattttaaat tgttgttggt ttattctatc
      541 ttcttaaaat agaataaatc tgaatttgac gggagggtag agcgtaaatt gaaacttgta
      601 aaagatagta tcagtgaaca aatctattcc ataataaaag aggaaatact gcttgctaag
      661 cgaaagccag gggagcaaat tagtccccga aagctggccg atgaatttga aactagtgtt
      721 atgcccatca gggatgcttt aaatcagttg gctaatgaag ggttattagt tcgcaagccc
      781 cgggttggtt tttttgtccg ttcttttact cagcaagagg cacaagacct tatggaggcg
      841 cgaaaacttt atgagcttta ctgtttagag aagtactttg atagaataga ccgtaaaaaa
      901 ctgtcggatt atttaaatag gtctttagaa agaatagata tttcttataa ggaccgagaa
      961 ttcgatgagc tggatgaaaa tattcatgat ttaattgtta aggcttcaca taactcgtgg
     1021 ttgataaatg gctataatag cattagagat tatattattg ttcttaaaca tttagatatt
     1081 ggccgagttc aaatggctca aaaagaacat atatctttga tcgaggctat tttgaatgga
     1141 gacaaagaaa aggctaaaaa gactttggaa gagcacatac aaagagtgac cgaagagtta
     1201 acaatgaacc tgtctcctcg atgataaaag aagatgattt ataaactgag gaagaacagg
     1261 catagtgctg tgccattgtg atttannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnntttag ctaattttct gctaccatcg tagttaactt catgataaat aacaacaatg
     1441 ctgtttgtag atagaaaggt agcatttgag gtttatgagc ttcttttagg cagaaacttt
     1501 aattcttatt tacttggtag gtacatagtc tgtaggttga ttacaggtgc tttcatgagc
     1561 ctctgtttta ttgaggctcc caagagctaa aattggtttt attgttttcc tcttgctaca
     1621 gaaaaaactg tcatgattga gaatttcata tccctggtgg caaatactgg cttctatttt
     1681 caaagcgcta ctttagtttt ggaggaaagc tcggaggagt taatctcaat tatgcggtag
     1741 ttatagcaat tgataacctg ggtattaaaa agctggtctt ctcgattttg gttgtggtcg
     1801 tagatgtgaa cgtgaccgtg gaggtgatag cggggttggt attttttgat gatgtgggta
     1861 aaagtggaaa acccccgatg agcaaggtcg ttcccttcat gaactccttg agggggagca
     1921 tgagttacta aaatgtctaa aaatctcccc tggaaaaatt tacaccacca gagtgaaggg
     1981 agcagccgca gatatttaaa atacatctct ctttcagtat attgatggat gcctccattg
     2041 taccagtaag agccttctaa gccggcaata attacgcctc ggtatgtaat aactctttca
     2101 tctaaattta ttcctccggc taatattttc tttcctcctg aggggtcatg atttccatgg
     2161 acaaaaaaga gggggatatt gaggttggtg actatgaagt caagataatg ctcaggaagg
     2221 tctccacagg aaatgatgca ctctacgtct ttgaaccttt cctgacaaaa atcaccatag
     2281 ataagaggat ttatccggtc gcttataact aagattttca tttattgaat tatacaatag
     2341 gtttgaaatt atgttaagaa aaggtatcaa agtaaagctt aaaaataacc tgcacctttc
     2401 ttactctttg ggagctcctc aagaagttct aaaaactttt ctactacccg agggtcgaaa
     2461 tgatggccag attgtttttt aatatatttt aaagctttct cttgaggcca ggctgagcga
     2521 taaggtcggt cagaaattaa agcatcccat acatctatta ctgcaaatat ccgggctgat
     2581 agaggaattt cttctccttt cagtcctcga ggatatcctg tcccatccca gcgttcatga
     2641 tggtagtaag gaatatcgag agcgggatga agatatttaa tgggggagag caactcccag
     2701 gcatagacgg ggtgtttttt cataatttcc cactcttcag cagttaaggg gccaggtttc
     2761 agtaaaattc ggtctgggat agccatctta ccgatatcat ggagaagtgc tcctctacga
     2821 atatggatta agttttcagg actcactccc agagtttggg ctaattttaa agttaactca
     2881 gccactcttc gggtgtgtcc ttcagtttct cggtctcgaa gttccagtgc ccgggcccag
     2941 ccttcaatgg tagtatcata agctaaagtt aactcaatat gggagcgatt gagttcctca
     3001 aacatggcaa tgttattgat ggctatggct aattgccctc ctatggtgat tagaaattct
     3061 gtccaatctg gaggagggtt aaaagggagt cgatgaaaag ctatcagcac tccttcagtt
     3121 tcacctttgg ctactaaagg aacagcaaag agggaaagaa aattttctct ttctatgaag
     3181 gggggaagaa cctttttctc ttcctcctca atgtgagaaa taaacagggg ttttttctcc
     3241 agcaccgctt tacccagggc tccttcttcc agccggaact ggtaagaagc tgcctcagga
     3301 aagagaaatc ccagacccgc tttatagcgt aaaacggagg agtcaattaa ataaatgccc
     3361 atagcatcta tttttaaatg acgcagagct tcttgcaagg taaaatccag taccatttgg
     3421 aggtctaagc tcgaggcaat ggctcggtcg atggaatgga gactgcgcat catatttatg
     3481 tatctctgta attgcgaaaa gagtcgagat tgttcaccag aggtaaccac ttgatgaatg
     3541 aaagtctgga taaaaccaga tagtgaagat agaaaatcag gtttatctac caaccacact
     3601 aaagctcccc aactatcagg agttttaccc agaagaaaag cttctacttc ttttatttcc
     3661 aactctttag ctattttttc taactgagga tatttagttt cctcggtaaa gttcaccact
     3721 aaagaagaac cgctgtgcaa caagtcagct aagaattggt ctttttccgg agaatctaac
     3781 caggactgga gaataggaga ataagaggaa ggaaaggtag agatagtctc ccacttttgg
     3841 ttgtctttaa gaattaggaa ggaggctacc tttaccttaa actcctgagc agaatagtat
     3901 actgctagtt gagctaattt tcctaaatcc agggttccgg agagttcttg agctccctga
     3961 tagagatggc tgagcatatc cagctggaaa gcaatctgtt gttctgctct cttgaggtcg
     4021 gtaacatcca ctaaggaaat taccgattgt ttagtgtggg gaatgaggtc tacgctggct
     4081 aaaacctccc tgacatttcc cgactgatct ataaggtgag tggtatattt tttaggaact
     4141 atttctggat cgacgcggcg tttttggtgg tattctatca tctgggaaac atcaggagga
     4201 gcaaaaaact gggtgaattt cctcttccct tctatttctt ctttaggaaa accggttaat
     4261 ttttcaaatt cccggttagc taaagagatg gtcatgtctt cctctactaa gatagtagcc
     4321 gaccctgtgt tttcaaaaat agtccgatat cgctcctccg attgagaaag ttctctttgg
     4381 gctttcttta actcagttac atctctaatt aaagctaccc aggctaatat ctcgccagcc
     4441 ctttgaatgg gggaaaggga ggcttctaag tagagttctt tctcgctttc gggataaaga
     4501 gtaaaggagt ggtttattct tgagtcttcc tgaagggctt cttctgggga aaaaaggtgg
     4561 ggtaaaagag accggatagg taatcctttt atctccctct cttttttccc tatcagttga
     4621 caagaagcag ggttaaccag ctgaactatt ccttctcggt caaataccaa cagagcatca
     4681 ctcatattat taatgatttc ctgggcagcc aaagcaggag ttatatctac taagtgataa
     4741 taccagatag ccggtgcacc taaaaggaca aaagaaaaaa tggggataaa acccagagga
     4801 gggataggaa cacctaaagc gggtaaaaaa tcaatggttg ctaagctggc tatcaaaaaa
     4861 gctcgaaaga aaagtctggt tcggagctta gccatgggag atatctcttc ctggctcttt
     4921 ttataatatt tcaggagtcc cgtaagtaac atatagaaga aaaaaactaa aaagggaata
     4981 cttatccatt cataatgagg ataatatccc caggaatgta attttacccc agagataaaa
     5041 ctctcgctta cgagagcaca cagggagaaa aagaaggaga gaatccatgc tatccaggcc
     5101 ttttgctgct gggaataagg ggcccgaaga gcaaaaatgg aaaactggaa aagggccgta
     5161 gaaataaaag gtactcccaa gaaagctaat ctcgaccaca caagtgctgt ttcctcatcg
     5221 ggagatacat acataaaaga gttagggaac agccacagaa aaatagagaa agtaattaaa
     5281 aagaagagaa tgctctcccg tgaagctttt tctcgaatta aaattcttat tccggtggag
     5341 agaattgtca ggctggtgaa gaggactgcc agagcaaaga tagtaaaatt ataatcagca
     5401 acttggagta taacattttt aaatataatg tcgaacaaaa tggcagcaaa cctcctcaat
     5461 aacagataag ctaaccagct ttacttcatt ccttcgttcc tccccgcgag cttactagac
     5521 ttatcccacc tctgctctaa agaattcaga gcaggatgtt tttcgcatcc attgtattat
     5581 acactttgtt gataaattta ttccttaatc gggatttaaa tagtaaaatg ctcaaggtct
     5641 tcttgtagcg ctcttcccat atccatagtt tcctgaattt ctttatccat ctcttgtaag
     5701 gcagcacttt gctcttccgc tgaagcagct acttcctcag ctgcggcagc attttcttcc
     5761 gaaatggaag ccacattact gagtctagta aatacctctt cggcctttcc taccacttct
     5821 tgagtttcta tttcgttctg atgagatatt tctgacattt ctctaactaa ctggacaatt
     5881 ttttgagata tttcttctac tcggaggaag gaagtagaga tagcattggt ttcttcagtt
     5941 accacttggt tagcttgata aatatcctga aaagcctgcc gagctctatc tactgattta
     6001 gttccactat ccactctttt ggtactttcc tccatagagc tcactgcttt attggcttct
     6061 ttttgaatgt ctccaattaa accagcaata cgctgagcag catgggctga ttcttcagct
     6121 aattttctca cctcatcagc caccacagca aaccctctcc ctgcctctcc tgctcgagcg
     6181 gcttcaattg ccgcatttaa ggctaaaagg ttagtttcat cagcgatgcc ggttataagg
     6241 tcagtgatgc ttccgatttc tcgggaactt tgccctaacg tctttactat ctctcctact
     6301 gagaggacgg agtggcttat gcttcccata gcttctacta cttcttttaa ggttttttgt
     6361 ccttcttcag agaggctgaa agcttgagta gcagattcct gaaccttttc taagttacca
     6421 ccggtgagct ggatactttc cataacctgg tctactgctt ctaaagtgat agaagctttg
     6481 tcggattgct ttttcaattg ctccgccatt cccttcactt ggcctactag atcctccata
     6541 ttttgggaga cttcggttaa attctggctt tgttcttctg accctcgggc tacttgagag
     6601 atggtttgac caatctggtt tccggtttga gcaatttctc gagaagcgtg ttgtagatct
     6661 tccatagaag atatgatttt ttctgctcct tcagctactt cttttagaaa agctcgtagg
     6721 gaccgagaag cctggtttag agagttaaag agagttccca gctcatcccg gcgacggaga
     6781 gaaatttctt gagtcaagtc tcccttttct aaaacctgag caaaagaaac actgcgattt
     6841 ataggagaaa caatgcctct ggagatgact atacctaaaa taatggccag aattactata
     6901 atacccacta ctaaatatag aatacgagat aactggtttt gaagctcact gacatgggta
     6961 acagtggtat caaattgatt aagtacttcc tgattcattt cattcattgc tgctaaaact
     7021 tgttggcctg ttttcgatgc ctcagtaata gcttttttgg tttgctcatc agcaaatttt
     7081 agataatcgt ccgcggcagt tactttattc cagagttcaa caaaaggtct ccagcgatcg
     7141 ttaattactg cctcagcagt aactgaccca taaatcttca gaaaatcttc ttccataaat
     7201 tttttcattt gttgcatatt tgtttctact tttttgcgag aggattcttt tgcttctgaa
     7261 ttggatagta agagataatt ttttctctcc tggttaatga tgtttaattt atctaaccag
     7321 tcatccattc ttaagtcttc taaagttctt aaaagagagc tataagaacg atggcattcc
     7381 atatataaat tgtctcgctc ttcaggagtg gcaatggaga agttgttttg taagtttttc
     7441 cagctttgct gataaagagc taatttattt attatatctt ctttcgtctg gtcagaaatc
     7501 atagaagaag tactgatatc gttggaaaaa gagtcaaagt aagagctgat agaaacagtt
     7561 attctcccat cagggtcacc ggtagctaac cagcgagann nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnntt aaactcctct gattctgttc ctaatctcag agcttcaata
     7741 taggtcataa ttttattatc taattgatta aattcttctt tcatttgatc cagagtagct
     7801 atgtcttcct ctaaaagata agaagatacc agagaatctt tctccgaaag aagatcttta
     7861 atcttagaag tggctaaaag cagaggagta ttatttctta cctcctctaa agaagtgcct
     7921 atttctcgaa aagcataaat agcaaagcca ccaactactg ctgctaacag cgctaagaca
     7981 atgaatccta aaggaattct ggttcggatt ttcaacttta tccctccaat tttatatatt
     8041 tctatattag ttaatcggct aattgaccca aaactttata atatttggat gtccaatcaa
     8101 aaatttatcc taaaatagtg tccagaatca tcatcactat aaatcctaaa aggagggcat
     8161 aagtggagga gcgagcatgg gaagaatgat gggtttcagg tattatctca tcgctaatta
     8221 caaagagcat tgctcctgcg gcaagagcca tccccagagg aagaaaggag tgcataatgg
     8281 taacaagaga aattcccaaa agccccccaa caggttctac taatccggta agtagagcat
     8341 aagtaaaggc ttgacgttga ttataaccta ctcctaaaag agaaacggct actgctagac
     8401 cttcaggaat attttgtaga ccaatggcca gggctactgt aattccgttg gatatgtttc
     8461 cgctgccaaa gcttactcct actgctaaac cttccgggaa attatgtaaa gttatagcca
     8521 ggacaaatag ccagattcgt cggaggtctg actctaatcc ttctcttcct ctctcaaagt
     8581 gttcatgagg tattatttta tcaaagacat cgagtaatac tcctcctatg gctactccca
     8641 gtgcagttat ccaggctcct cccatttcta ttgccgggac taaaagacta aaagcagtgg
     8701 cggcgagcat tattcctgct gccactccta atagttcgtc cttgaattga tgggaaatct
     8761 tttgccctct cccaaaaaga actggtaatc ctccgatacc agtagctaaa ccggctaaag
     8821 tactggctaa agttcctaaa agaacaggat tcataagtat cacctttttt tatttttatt
     8881 ctctcttaat ttctcggaga tagcaagtaa tgtaagctta aagatagcac taactatatt
     8941 tttactgatt taaggtttga acttctattt attcagcaag tcatgaaatc cttttttctg
     9001 aaaaataact ttctttattt tctagttctt ttaaaatata gtgtacatct ttacaagact
     9061 ttatctttat ataaaggttg gcgcttagca atttatatat ttactaaagc tggatggatg
     9121 aaccttcaat gactttgcac tgacttcggg gaataaattt caacaaaaat agagggaaaa
     9181 tgataaaata tagaccaaaa acaatgggaa ggagaaaaaa atgaaaactt attgcatagg
     9241 tgtgatacct ggagacggaa caggaccaga agtaataaga gaagggctca aagttttaga
     9301 agctatttct aaaaaatcgc atttttctta cactactatt ttttatccct ttggaggaga
     9361 acattatcta aagacagggg aaaccttacc tgattcagcc attgaagaat tcaagaaatt
     9421 agatgctctt tatttaggag ctataggaca tcctgaagta aaaccaggaa ttctggagcg
     9481 gggaatttta ctgaaattac gattttcttt agaccagtac attaatctcc gaccggtaaa
     9541 actttatcct ggagtatgga cacctattaa agacaaagga ccagaagata tagatttcgt
     9601 ggtggtaaga gaaaatactg aaggtttgta cgtaggagct ggaggtaact tgcatcgcgg
     9661 gactcctcac gaagtagcaa ctcaagaatc tattaacact cgtcggggag tggaacgatg
     9721 tattcgtttt gcttttgaat ttaccaaaaa aagaaataaa aaaaataaac tcactctgtg
     9781 cggcaaaact aatgttttaa cttatgcttt tgatttatgg gaaagaactt ttcaagaagt
     9841 agctcaagaa taccccgata ttaaaactga ctacgctcat gtagatgcta ccaccatgtg
     9901 gatggtaaaa aaccctgaat ggttcgatgt aatagtaact gacaatatgt ttggcgatat
     9961 catcaccgat ttaggagcta tgattcaagg tggcatggga atagcagctg gtggtaatat
    10021 taatcctgaa ggggtttcta tgtttgagcc tattggaggt tccgctccta aatatacagg
    10081 gaaaaatatg attaatcctc tggcagctat ttctgctttg caaatgctat tgtcccatat
    10141 aggagaagag gaagcagctc aaagagtaga aaaagctata atcaaagctt tagaaagcgg
    10201 aaagatcaga agcatgagtg ccggacaaat gggacttacc actactgagg tgggagattt
    10261 aatagcttcc ttagtttaaa attaatctaa aggtgttcct ccttcctgca cggggggaat
    10321 gctcgtagag gaagatggct tttcagttgc tccttctgta gagggttttg cctcagagga
    10381 aggagcaacc tcaagaggtg ttcctccctc ctgggtagga ggtttacctg cagaaggtgg
    10441 ggtgctttcg agaggtgttc ctccttcttc agccggggta ccaccagaag ttattcctgg
    10501 tgaagagact attactccct gtcctgagcg aagagcaggt ttttctttag aagaggcgat
    10561 aaaccgttgc acttctattt ctaccaaatt aaaattatca tgtttctcta aagcattggc
    10621 taacacgcta gctacttgtt ccgaatccac tcctgctttt cgagcgtttt caagaagttg
    10681 ggccatcttt ttcatatctc gagaggaaag atttttatct gctgctgctt tcacaatctc
    10741 ccctgccttt tccggatcta ctcccatttc taagagagag gacaaggaat ctactatcag
    10801 ttgcaagcgt ttcccatcgt tagctaaagc ttgacttaaa gcctcttcta ttgcctgggg
    10861 cggaacggat aattctaaag ataaagccag atcaattatc aaattctctt ctccttttat
    10921 accatttttt tgtaaaatct cctgagcttt ttctagagag ccttttttat tattcaaagt
    10981 ttttactaat tcatgaggga gaactttctt gcttaaacct tctttaagtt tggaaataat
    11041 gggagaaagg ggaagacttt tttcctgtgc ttcttttatt aaagaatcta cctggctccg
    11101 aagatattct ttgtcctctt ctgagtaagg ggatttttca ataagtgaaa gagggtcact
    11161 tgccgcccat agaggaacta ttcctgcaaa gaacaaaaag aagatgaaga acgaaacaaa
    11221 aatctctttc ttcatgagtt tgattcaccg ttaaccacca gtaccgagtt aaatttcccg
    11281 aacccatcgg ggacttgccg gagagaattg ggatcagcta cccattcttt accatctact
    11341 aagaaaccat attgatattt acctggtttg acttttaatg ttattctcca acaatcttct
    11401 ttaactcttt ccatctgcag gtaatcccag ctactaaaat ctcctgctac tgccactgtt
    11461 ctagaactct gggaaggaga ataaaaaacc agctctattt tccggtaacc cagagaagga
    11521 gaaatctcca gctcggggag cagaggaagg ttactaaaga aaatcagaac gagaaacgca
    11581 gctgctaagg aaaccactac ccattttttg taatcagcat aatgcaaatg ctttttttta
    11641 cgtccttcta tccgatacat aacttgctga gcaaaacctt cacttacctc aactttaggt
    11701 aagttttcta gatattcaac aagcttcttt tcttttatat ccatactcct cacctgattt
    11761 cttatacgta aaacagggct gttcagtttc aaggatgggt tttttgcctg atttattact
    11821 ttccctgcaa aaatacataa gaacctatca gctttttgtt aaagttgaaa aaagtggctc
    11881 tccgtgagat aatccattat aatgactatg agggggaaaa agacccccct cataaccccc
    11941 ccaaaaggca aaaaagttaa aaacaactga gggagactct ttctccctca gtggggcggt
    12001 gccccctcat aacccccaaa agcaaaaaat taaaaactga aaagacaaag actactgagg
    12061 aagaaaac
//
LOCUS       DAOSVE010000050        12067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_3045_length_12067_cov_56.176740, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000050 DAOSVE010000000
VERSION     DAOSVE010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 12067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12067
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3045_length_12067_cov_56.176740"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..1089)
                     /locus_tag="PK016_05445"
     CDS             complement(148..1089)
                     /locus_tag="PK016_05445"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HXL02547.1"
                     /translation="MEKNWREKLEKASLSRIMEVAYENQVLVPAFNVAYLPMVEAIIE
                     ALRETETFALLEVSRPDITRFGAKSFQTVKEEYERYGDLAFSRLHQDHVPVIDEEGEK
                     VDWKSLIEEALSLGYHSVMIDGSRLPLEENIRITRETVMLAHRRGVCVEGELGSVLGH
                     EKGPLPPYEELFQSGRGFTSPEDAQRFVQETGVDWLSVAIGNIHGAITGAGKNQKKVE
                     ARLNIGHLQQIARVTGIPLVLHGGSGIRREYVLEAIKNGITKINIGTEIRQAYERGLG
                     ESSNIKEAQKETKEKVTELISSYYEIGGSVHILSEKL"
     gene            1502..2464
                     /locus_tag="PK016_05450"
     CDS             1502..2464
                     /locus_tag="PK016_05450"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXL02548.1"
                     /translation="MKRKKVLVIGGTGHIGSYLIPSLFARDYEVLVFSRGKTTPYPES
                     FWEKEVKMLTVDREKEEQTGHWQALIEEIQADIIIDIICFEPESAQILIDTLTGKDVH
                     LLSCGTVWVYGKTRIVPTPETAPRLPENDYARKKTIIEDKLLQATREDKIKATIIHPG
                     HITGPGKTFVTPFGDHNPETLQKIIDGKEIYLLDGGFSTLHHVHPQDVASLFLRAIEQ
                     ENKTIGESFNCGAPEAMTFWGLGEYFASLFGKKFYFQNISLEEYTEKFGYPEEAAQHV
                     RQGCCVSMLKAQEVLNFAPHYSAREAVASATDDLIKRGILKVNK"
     gene            2461..3825
                     /locus_tag="PK016_05455"
     CDS             2461..3825
                     /locus_tag="PK016_05455"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HXL02549.1"
                     /translation="MSIFKDCDIRGVFPTEINEEKAYFIGRAFASSLPAESRIVVGGD
                     VRESTLILKEALIEGLLESGGEVVDLGIIPTPLLYFAADKLQSKGGIMVTASHNPPEY
                     NGFKITRGNVPPTPEDITNLAKKVKGKNFRHSKKGKVISQNLDREYLDFLSSLTPRPH
                     RPLQVVVDCGSGCYSDLAPDFLKNLGYEIIPLFCEKDGTFPHRSPNPSQEGNLAALSI
                     KIKEEAANAGIAFDGDGDRVVFADEKGEVLKGEEGIIFFLRNIMPHLPRGEKFVYDLK
                     CSQIVPQEVKRMGGIPIPERSGHAHIKRRLLQENAFMAGEISGHYFFREIKRDDGLYA
                     AALFLYHLSQASHALSQIRETFPPSYVTPDIRIAARGRENLLSILENSISGGEVSYID
                     GIRVKWEEGWAMIRKSVTEPVYTLRFEGKTCQDIPQLVHRFLQPLPEIEEEVLHQIKK
                     QGRN"
     gene            complement(3822..4304)
                     /locus_tag="PK016_05460"
     CDS             complement(3822..4304)
                     /locus_tag="PK016_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HXL02550.1"
                     /translation="MTYSAMAIALVAIATMSLQIPIPQTRGYINLGDTLIFVFALLWG
                     SRVGALAGGLGSALADLLTGYAHWAPFTLVIKGVEGFLVGALARREPKLLQRILFLSL
                     AGLEMVGGYFLAGSLLYGPGVAIVEIPGNLVQAGSAILLSSLAAYAIRRGEKYIFHRV
                     "
     gene            complement(4376..4741)
                     /locus_tag="PK016_05465"
     CDS             complement(4376..4741)
                     /locus_tag="PK016_05465"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HXL02551.1"
                     /translation="MDNGKEETRDFIKEAEELLVDAENDLNRGDYKSCFSKSYHAMVS
                     SLKALLNLLEKRQEKAEVEKLSREVESKFEDIYNQILLIDQATGLVLNEKEVREFLKA
                     AGNFVQDLKNRIKTFSFPA"
     gene            complement(4787..5350)
                     /locus_tag="PK016_05470"
                     /pseudo
     CDS             complement(4787..5350)
                     /locus_tag="PK016_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035236.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
     gene            complement(5792..5932)
                     /locus_tag="PK016_05475"
     CDS             complement(5792..5932)
                     /locus_tag="PK016_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02552.1"
                     /translation="MKSDKEEKEYWMMKEARGLIKERFGVKFSEGQIVKMFRYKLKYT
                     CL"
     gene            complement(6068..7069)
                     /locus_tag="PK016_05480"
     CDS             complement(6068..7069)
                     /locus_tag="PK016_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL02553.1"
                     /translation="MEHQKSFSKRKLSITFKWEWLLVILIIGISVVNGRLSSHFWSYT
                     GIMDALSVFLEKGFMVLSMALVLIIGDIDISVASIVALSSVSMAMSYKAGVPMAVAIL
                     IALATGAGCGYFNGLLISRIPGLSAIIVTLAGSSLYRGIAYILLGDQAIGGFPSWYSY
                     LAWGYIGRTHIPFIIVVFVGCAIIYGLLLHRTAWGRKVYAMGINPVAARFSGVPVAKI
                     KQIIFALNGLMAGVAAIFLTSKLGSSRPNIATGYELEVIAIAVLGGVSPSGGKGGIVG
                     VCLALILMRLLRYGMGLVNVPGQVMMVIIGIVLIGVVVFPNVFSSLKVRKREVKEIQ"
     gene            complement(7070..8083)
                     /locus_tag="PK016_05485"
     CDS             complement(7070..8083)
                     /locus_tag="PK016_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019004826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL02554.1"
                     /translation="MTNEPAKTKKEVAFSRLPGFRELGIIIFIILISVLIGIRNPRFL
                     TWGNFHDILLDTAALFVLSVGMMLVLVTGGIDLSMESVLALTGMVAGMMVKQNPQLSP
                     LFMLLLGLILGALLGSITGLIVAKGRVVPIIATLSMMYIYRGITFIISGGKWINAYEM
                     PDSFKGIATERFLGLSKLIYIVLVVYLVFYYFVNHTRIGREIYAVGSNKEAARIIGIN
                     TQFITWLVYVISGALSGLAGVMWVARYASAQNDTASGFVMTIVAACVLGGVSITGGVG
                     TIPGILLGSITVGIINNALPMIRVSPFWKMALQGLIILVAAIVNIAITRNMEKSQLKR
                     RVI"
     gene            complement(8109..9635)
                     /locus_tag="PK016_05490"
     CDS             complement(8109..9635)
                     /locus_tag="PK016_05490"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015255795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HXL02555.1"
                     /translation="MVDAEDRYILELKGISKFFPGLKALDRVDFNLRAGEIHAIVGEN
                     GAGKSTLIKIVTGVHQPDEGNMFYEGNKVIFHNPMVANRYKIAAVYQQPASFPHLTVT
                     ENIFLGHPVIHSTTKRLMWKEMRQEAKRLLRSLGSDINPSVTMSSLSVAQQQIVEIAK
                     ALSVNARVLIMDEPTASLTRRETEDLYNIIRKLRNDGVSIILISHRFEDVYEVADRVT
                     VLRDGKSIGTWNVSEIGEKDLIKAMVGREINQLFPKSAVPFGKELLRVEGLSRVGYFS
                     DVSFSLRAGEILGFSGLVGSGRTEVAQSIFGIAPADAGKILVEGKEILIKNPMEAISY
                     GIGYLPEDRLEQGLFLLLSINDNITISIIDQLTNKGWLNPPREYEISSKMLELLQIKA
                     PGIFHPVGSLSGGNQQKVVVAKLLVAHPKILIMDEPTKGVDVGAKAAIHQIMSDLVAE
                     GLGIIMISSEMPEILGMSDNIIVMHEGRITGVFSRSEATQEKIMEAAVKIDRSVQVVS
                     "
     gene            complement(9701..10708)
                     /gene="rhaS"
                     /locus_tag="PK016_05495"
     CDS             complement(9701..10708)
                     /gene="rhaS"
                     /locus_tag="PK016_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0033296 - rhamnose binding [Evidence IEA];
                     GO_process: GO:0015762 - rhamnose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnose ABC transporter substrate-binding
                     protein"
                     /protein_id="HXL02556.1"
                     /translation="MKKLFPVLFLSLSMVVLFSVSAFAGEHTIGMVVKNVGNPFMDAA
                     GRGAEEACNELGNSFIFQGPATPTVEGQIEIIENLIAQEVSAICITANDFDALVPVLQ
                     KAKGNNIKVVSFDSAVAPGGRDIHVNQANAELIGKLQVQAIAEMIGYEGEIAILSAGA
                     TMTNQNTWIEYMKEELKDPKYAKMKLVTIVYGDDLRDKSYNEAMGLFKSYPDLKGIIS
                     PTTVGISATAKAITDAGLVGKVKLTGLGLPSEMAEWVHNGACEAFFLWNPVDLGYLAS
                     YVAGLLCDGVITGKLGEEFSAGRMGKYTIVEADDGGTEALLGPPFRFDISNIDEWKDV
                     Y"
     gene            complement(10945..11715)
                     /locus_tag="PK016_05500"
     CDS             complement(10945..11715)
                     /locus_tag="PK016_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HXL02557.1"
                     /translation="MFSEERRLKILELLDEKRTITVKELSDVFGVSEDTIRQDLKILE
                     EQRLLRRIYGGATRIKRGVVELPFTLREITNREIKELIGKKAAKLIEDGDSVVFDAST
                     TALQVARNISPGLQITILTSSLDICINLAQNRKFNIISTGGTLHPPSFSFVGPQAENA
                     VLNYHADKLFIGTKAISVDKGYIADTFELEAHLKKVMIESAQEIILVAESRKFEEVAF
                     FRVCKLDCISKIVTDSNIKKETLTALKDLGIEVLISND"
BASE COUNT         3791 a         2773 c         2014 g         3489 t
ORIGIN      
        1 atttactttt taaaagtttt aatattttaa ccagttttgc gggatactat aatagtggaa
       61 ggttaaaaag cataaaagta aaaaagactg ctgagggaga aaaacctccc tcagcataag
      121 agcaaatttt ttgtagcttc ctctttatta taatttttca gaaagaatat gtactgaacc
      181 tcctatttca taataggagg atatcagttc tgtaactttt tctttcgttt ctttctgagc
      241 ttcttttata ttagaacttt ctcctaaacc tctttcataa gcctggcgaa tttcagttcc
      301 tatattaatc ttggtaatgc cattcttgat tgcttccaga acatattctc ttctaatccc
      361 tgaacctcca tgaagaacca gaggaattcc ggtcactcgg gcaatttgct ggagatgtcc
      421 gatatttaat cgagcctcta cttttttctg gttctttcct gctccagtaa ttgccccatg
      481 aatattacca atagctaccg aaagccagtc tacaccggtt tcctgaacaa aacgctgagc
      541 atcttcggga gaagtaaatc ctcttccact ttgaaaaagc tcttcataag ggggaagagg
      601 acctttttca tgtcctaaaa ctgagcccaa ttctccttct acacaaactc cgcgtcgatg
      661 agcaagcatt accgtttccc gagtaatacg gatattttct tccagaggta aacgagaacc
      721 atcaatcatc accgagtggt atcctaaaga aagagcttcc tcaattaacg acttccagtc
      781 tactttttct ccctcttcat ctataactgg aacatgatct tgatggagtc ggctgaaagc
      841 caggtctccg tagcgttcat actcttcttt taccgtctga aagctctttg ctccaaagcg
      901 agtgatatca gggcgagaaa cttccaaaag agcaaaagtt tcggtctctc gtaacgcctc
      961 tataatggct tcaaccatag gaaggtaagc aacattaaaa gcaggaacta acacctggtt
     1021 ctcataagct acctccatta tccgggaaag agacgctttt tctaattttt ctctccaatt
     1081 tttctccatg gccatcattc tcctttccat ttctaaccat ttctaaccat ttttaattat
     1141 agttaaccgc aaaatccatt ataaagacta tgagggggaa aaagaccccc tcataacccc
     1201 caaaaaagat taaaacttga aaaatataaa agtaaagact caaaaactac tgaggaagaa
     1261 agagtctccc tcagtgggct gtgcctcctt ataacccccc aaaaagcaga gaacaaagaa
     1321 gcaaaatgct actggaaata ttatatagta tccctcagta catagcgacc ttgagttttt
     1381 gacttccggt gtaatttact ttttaaaagt tttaatattt taaccagttt tgcggaatac
     1441 tataactatc ttccagatat aactataaaa aggtaaaata gtaaagcaaa ggaggaaaaa
     1501 catgaaaagg aaaaaagttt tagtaatagg aggcacagga catatcggta gttatctaat
     1561 tccctctctg tttgccagag actatgaagt attagtattt tctcggggga aaaccactcc
     1621 ctacccggaa agtttttggg aaaaagaggt gaaaatgtta acagtagata gagaaaaaga
     1681 agaacaaact ggtcactggc aagcgttgat agaagaaatt caggccgata tcataataga
     1741 tattatatgt tttgagccag agagcgctca aattcttata gataccctga ctggaaaaga
     1801 tgttcatctc ctttcctgtg gcacagtatg ggtatatggt aaaaccagaa tagttcctac
     1861 ccccgaaact gctcctcggc ttcccgaaaa cgactatgct cgcaaaaaaa ctataataga
     1921 agacaagctc ttacaggcta cgagagaaga taaaattaag gctactatca ttcatcccgg
     1981 gcatattacc ggcccaggaa aaacttttgt tactcctttt ggagaccata atccagaaac
     2041 tctacaaaaa ataattgatg gaaaagaaat ttatctattg gatggaggat tttccactct
     2101 tcaccatgtt catcctcaag atgtagcttc tcttttccta agagctatag aacaagagaa
     2161 caaaactata ggagagagct ttaactgtgg agctccagaa gccatgactt tttgggggtt
     2221 gggagaatat tttgcctctc tttttggaaa aaagttttat ttccaaaaca tttctttaga
     2281 agaatatact gagaaatttg gttatcctga agaagctgct caacatgtcc gacaaggttg
     2341 ttgtgtatct atgctaaaag ctcaagaagt tttaaatttc gctccccatt attcagcaag
     2401 agaagcagta gcgtcagcta ctgatgattt aataaaaaga ggaatattga aggtgaataa
     2461 atgagtattt ttaaagattg tgatattagg ggagttttcc ccaccgaaat aaatgaagaa
     2521 aaagcttatt tcattggtag agctttcgct tcctcacttc ccgcagaatc cagaatagta
     2581 gtaggaggag atgtgcggga aagtactctt attttaaaag aagctctcat tgaagggctt
     2641 ttagaaagtg gaggagaagt ggttgatttg ggaataatcc ctactcctct tttatatttc
     2701 gctgcagata aactacagag taagggagga attatggtta ctgcctccca caatccccca
     2761 gaatacaatg ggttcaaaat tacccggggg aatgttcccc ctactcctga agatattacc
     2821 aatttagcta aaaaagttaa aggaaaaaac tttcgccaca gcaagaaggg aaaggtaata
     2881 tcccagaact tagatagaga atatctcgac tttctttctt ctcttactcc ccggcctcac
     2941 cgccctttac aggtggtagt ggactgtggc agtggatgtt attctgacct tgcccctgat
     3001 ttcctgaaaa atttaggcta tgagataatt ccgcttttct gcgaaaaaga tggaacattc
     3061 ccccaccgct ctcctaaccc ttctcaagaa ggcaatttag ccgcgctctc tatcaaaata
     3121 aaagaggaag cagcaaatgc agggatagct tttgatggag atggagaccg ggtagtattc
     3181 gcagatgaga aaggagaagt tttaaaagga gaagaaggaa ttattttttt cctccgcaac
     3241 atcatgcccc acctcccccg gggagagaaa ttcgtctatg atttaaaatg ctctcaaatt
     3301 gttcctcagg aagtaaaaag aatgggagga atccccatcc ccgagcgctc tggacatgcc
     3361 cacattaaaa ggcggctact ccaggaaaac gcttttatgg caggggaaat cagcggtcat
     3421 tactttttcc gagaaataaa aagagatgat ggattgtatg ctgcagctct atttctttat
     3481 catctttccc aagcatctca tgctctttct caaataaggg aaactttccc tccttcctac
     3541 gttactcccg acattcggat tgctgcccga ggaagagaga accttctctc tattctagaa
     3601 aattcaattt caggaggaga agtgtcttat atcgatggaa taagagtaaa atgggaagaa
     3661 ggatgggcaa tgattcgtaa atcagttacc gaaccagtgt acaccctacg ttttgaggga
     3721 aaaacttgcc aggatattcc ccaattagtc caccgcttct tgcagccttt acctgaaata
     3781 gaagaagaag tgctccacca gattaaaaag caaggaagaa actaaacccg gtggaaaatg
     3841 tatttttctc ctcgacgaat ggcatacgct gctagagaag ataaaagtat ggcacttcct
     3901 gcctgtacca aattacctgg tatttctact attgccacac caggtccata aaggagagag
     3961 ccagccaaaa aatatcctcc caccatctct aaaccggcca aggataaaaa gagaatccgc
     4021 tggaggagtt ttggctcccg gcgggctaaa gctcctacta agaaaccctc cactcctttg
     4081 attacaagag taaatggagc ccaatgagca tatccggtca aaagatcagc taaagctgag
     4141 cctaaccctc cagctaaagc tcctacccga gacccccaca gcagagcaaa aacaaatatt
     4201 aaagtatctc ccaaatttat gtatcctcgg gtctggggaa tgggaatttg taaactcata
     4261 gtggctatag ccaccagcgc aatagccatt gctgaatatg tcaaagtccg ggttgactct
     4321 ttcgttgtaa tctcgacctc ctcttgctgc gtctacagtt attttatcat agtcatcaag
     4381 cgggaaaaga gaaagtttta atacgattct ttaagtcttg gacaaaatta cctgctgctt
     4441 ttaaaaactc tcttacttct ttctcattta aaactaagcc tgtcgcctgg tctatgagca
     4501 atatctggtt atatatgtcc tcaaacttgc tttctacttc tcggctaagc ttttctactt
     4561 ctgctttctc ttgacgtttc tcgagcaagt tcagtaatgc cttcagcgaa gagaccatag
     4621 catgataact tttcgaaaaa caagacttat aatctcctct attcaggtca ttctctgcat
     4681 caactaatag ttcctctgct tcctttatga aatctctcgt ctcctctttg ccattgtcca
     4741 tatcgctatt actccatgaa tattttcgac attcattagg atcttcaaat agaacttctt
     4801 tgggatcaaa atattcagag tcgaactctc cgccaaccca ctctaacatt gtgctgtgtt
     4861 cctcatgctc aggatttctt atagcctcta aaaattcttc atacccccat acgcctccac
     4921 aatcttcagg aggacaggct cttttcccgg ctatgcatag aggatatttg acattcttat
     4981 ctcgagggag tattttttct aactctattt tatgttccca gccatctcca aaatcataaa
     5041 tataatttgc tgtcttattt tccatagaaa accagttagc aatcttctgc ttttcttcgg
     5101 ggataatttt ttgcccaaaa acctcatcct cttcgctggg gtttcctatc ctcactttct
     5161 ctcctgtaga aggagagacc atttcaaatt catgaagatg agtgtctgtc caacccatgg
     5221 catcctggat agcaatatgt aagtcccaaa aggtataagt ttcaggaact tgtattctcc
     5281 gccagattgg tggtctgata cctttgagag taatcttaaa ctgatatacc ctatcaaatt
     5341 tctttttcat taaaaccttc cccttatttt aaagtttaca tatattttac tgacgtttcc
     5401 tagatttatg aagctcagct ttacggaatt aaaatagaat acaaaagcca tttatccgct
     5461 cattagctgc gcatcgtctt attcttccat ctttaattcc ttctccttta acaaagggaa
     5521 ggcaagttac aatattttct aatattactt cagtgccctt tgaccttcca taattcacag
     5581 tacaaagttt atagttaacc gcaaaatcca ttataaagac tataaagggg agaacccctt
     5641 tataatcccc caaaaagata agaaacttaa aaaaacaaaa gaaataaagg ccatagagaa
     5701 agacaaagtc tttctctatg cgtagcgacc ttgagttttt aactttcgat ataatttact
     5761 ttctaaagtt tttaatcagt tttgcgggat actataaaca agtgtatttt aacttatacc
     5821 taaacatttt tactatctga ccttcagaaa actttacacc aaacctctcc tttattaatc
     5881 cccttgcttc cttcatcatc cagtattctt tctcctcttt atcgcttttc aacctgattt
     5941 ttatttctcc cggatcctgg tataaaattt tctcttttca taacagttta attacagcaa
     6001 atttgtgaga tgctataatt taaagaattc tgatgataaa gagagaacaa atttagtaca
     6061 ggaaaactta ttgaatctcc ttcacttctc tttttcgtac ctttagagaa ctaaaaacat
     6121 taggaaatac caccacacca ataagaacta ttccaatgat aaccatcatc acctggcctg
     6181 gcacatttac taagcccatg ccatagcgga gtaaacgcat caatatgaga gccaaacata
     6241 caccaactat accaccctta cctcctgaag ggctaactcc tcccagaacg gcaatggcaa
     6301 taacctcgag ctcatatcct gtagcaatat taggtcttga gctacctaac ttagaagtta
     6361 gaaaaatagc tgctacccct gccatcaacc cgttaagagc aaaaataatc tgctttattt
     6421 ttgctaccgg cactccagaa aatcgagcag ctactggatt aatccccata gcatatacct
     6481 ttcttcccca ggcagtccga tggagcaaaa gaccgtatat aatggcacac cccacaaata
     6541 ccactataat aaatggtata tgagtcctcc ctatatatcc ccaggccaaa taagaatacc
     6601 aggagggaaa ccctcctatc gcctgatcac caagcaaaat ataagcaatt cctcgataaa
     6661 gagaggaacc ggccagagta acaataatag ccgatagtcc gggtatgcgt gaaatcaata
     6721 aaccattaaa atatccacac cccgcccctg tagctaaagc tattaaaatg gcaaccgcca
     6781 taggaacccc agctttgtaa gacatagcca ttgacactga agagagagca acgatagaag
     6841 ccacagaaat atcaatatcc cctataatca ataccagagc catagaaaga accataaagc
     6901 ctttttctaa aaataccgag agtgcatcca taatgccggt gtaactccag aaatgagatg
     6961 aaagacggcc attgactact gatattccta taatcaatat aactaaaagc cattcccact
     7021 taaaagtgat gctaagcttt cttttagaaa aggatttctg gtgttccatt tatattactc
     7081 tcctcttcaa ctgcgacttt tccatgtttc gagtaatagc aatatttact attgccgcca
     7141 ctaagataat aagaccctgt aaagccattt tccaaaaagg agatactcta atcatgggaa
     7201 gggcgttatt gataatacca acggtgatag accccagtaa aataccaggt atagttccca
     7261 caccgccagt aatgctaact ccacctaaaa cacaggcagc tacaatagtc ataacaaagc
     7321 cagaggcggt atcattctgg gctgaggcat agcgagctac ccacatcacc ccggccaaac
     7381 cagagagagc gccggaaata acataaacca gccaggtaat aaattgagta ttgattccaa
     7441 taattcgagc tgcttcttta ttgcttccca cagcatatat ttctcttcca atacgagtat
     7501 gattgacaaa ataatagaaa actaagtaca ccaccaaaac aatgtaaatc aacttagaaa
     7561 gacccaagaa acgttctgtc gctatccctt taaaagaatc cggcatttcg taagcattta
     7621 tccacttccc accgctgatt ataaaagtaa taccccggta aatgtacatc atactcaaag
     7681 tagcaataat tggcaccacc cgtcctttag ctacaattaa accagtaata gaccccagga
     7741 gtgctcccag tattaacccc agtaacagca taaaaagtgg agaaagctga ggattttgtt
     7801 taaccatcat ccctgctacc attcccgtta gagccagcac cgactccata gaaagatcaa
     7861 tgccaccagt aactaaaacc agcatcatcc ccacagaaag cacaaataga gctgccgtat
     7921 ctaacagaat atcgtggaaa ttaccccagg ttaaaaaacg aggattgcga ataccaatca
     7981 aaacagaaat aagtatgata aaaattataa tccccaattc ccggaatcca ggcagtctgg
     8041 aaaatgcgac ctctttcttt gttttagcag gttcattcgt cataggttta caccatccag
     8101 atttaacttt acgacacaac ttgaactgaa cgatcgattt ttactgctgc ctccataatc
     8161 ttttcctggg tggcttccga acgagaaaaa acaccagtaa ttcgaccttc atgcataaca
     8221 attatattat cactcattcc caatatttct ggcatctcag aagaaatcat aataataccc
     8281 agcccttctg ctacgagatc agacataatt tgatggatag cagcttttgc ccccacatca
     8341 acacccttgg ttggctcatc cataatcaat attttcgggt gagcaaccag taatttggca
     8401 accaccactt tttgctgatt ccctcccgat aaagaaccaa cggggtggaa aatccctgga
     8461 gcttttattt gaagcagttc cagcatttta ctagaaattt catattctcg aggcggattt
     8521 agccatcctt tattggtaag ctggtcaatt atagaaattg taatattatc attgatactc
     8581 aagagcaaaa acaaaccctg ttctaaccgg tcctcgggaa gatatccaat gccgtaactt
     8641 atagcctcca tcgggttttt aatcaatatc tcttttcctt ctaccaatat ctttccagca
     8701 tcagccggag ctataccaaa aattgattga gccacttcag ttcttcctga accaaccaac
     8761 ccgctgaacc cgagaatttc accagctcgg agggaaaagg aaacatcgga aaaataacca
     8821 acacgagaaa gtccttctac tctcaaaagc tctttcccga aaggcactgc acttttggga
     8881 aagagctgat ttatctctcg ccctaccatg gctttaatca ggtctttttc accaatctcg
     8941 cttacattcc aggttcctat actttttcca tctcggagta ccgttactcg gtcagcaact
     9001 tcatagacat cttcaaatcg atgagaaatt aatataatag aaactccatc gttgcgaagt
     9061 tttcttatta tattatataa atcttcagtt tcccgcctgg tgagagaagc agttggttca
     9121 tccataataa gtacgcgagc attaactgaa agagccttag cgatttcaac tatctgctgt
     9181 tgagccacac tcaaagaact catggttacc gagggattaa tatccgagcc taaagaacgg
     9241 agaagtctct tcgcttcttg cctcatctct ttccacatca gtcttttagt agtagagtga
     9301 atcacaggat gaccaagaaa tatattctca gtaactgtta aatgaggaaa ggatgcaggt
     9361 tgctggtaaa cggcagcaat tttataacga ttagctacca taggattatg aaaaattacc
     9421 ttgtttccct cgtagaacat attaccttcg tctggctgat gaacgccagt aacaattttt
     9481 ataagagtag atttgcctgc tccattttca cccacaatgg cgtgaatttc tcctgctcga
     9541 agattaaaat caacccggtc taaagctttc agaccgggga aaaacttaga aatccccttc
     9601 agttcaagta tatatctatc ttctgcatct accacactgt taacccccct aaaatgggtg
     9661 ggcgagaatg attctcgccc acccatttac tgcaccaaac ttaataaacg tccttccact
     9721 catcaatgtt acttatatca aatcggaaag gaggccctag taaagcttct gttcctccgt
     9781 cgtctgcttc tacaatggta tattttccca ttctaccagc agaaaattcc tctcctaact
     9841 taccagttat aaccccatca cacagaaggc cagcaacgta ggaagcaagg tagcctaaat
     9901 cgacaggatt ccataagaaa aatgcctcac aagctccatt gtgaacccac tctgccatct
     9961 cactgggaag cccaaggccg gtaagcttaa ctttccccac taaaccggca tcagtaatag
    10021 ccttagcagt agcgcttatg cccacagtgg taggagatat gatacctttc aaatctggat
    10081 aggatttaaa caaacccata gcttcattat aacttttatc tcgcaggtca tcaccgtata
    10141 caatagttac taatttcatt tttgcatact tcgggtcttt taattcctct ttcatatact
    10201 caatccaggt attctggtta gtcatggtgg ctccagctga aagaatggct atttcacctt
    10261 catagccaat catctccgct attgcctgaa cctgcaactt ccctatcaat tctgcatttg
    10321 cctgatttac gtgtatatct cggcctccgg gagctactgc cgaatcaaaa gacacgactt
    10381 ttatattatt tcccttagct ttttgaagaa ccggaaccaa agcatcaaag tcattggcag
    10441 ttatacaaat agcactaact tcctgagcta ttaagttttc aattatttca atctgacctt
    10501 ccactgtcgg agtggccggc ccctggaaaa taaagctatt acccaattca ttacaggcct
    10561 cttcagcgcc tctgccagcc gcatccataa aaggattccc gacatttttc actaccatcc
    10621 caatggtatg ctctccggca aaagcactta ctgaaaagag gacaaccatg gaaagtgaca
    10681 aaaaaagaac tggaaaaagt tttttcattt ctaattttaa cctcctttat cctggttacc
    10741 agatttaact accaaaacta ctacacactg tcatcatcga ccagtaaacc cttactgacc
    10801 accaatcctt aagaatcctt ttctaccacc tccttttgtg accttcgctt ctttcatgta
    10861 acgcccatat tttttcacat tggtagacag ttttccataa ccatcttttt cctatcgcac
    10921 aaactaagag caagaaatta aagcttaatc attgcttatt agaacttcaa tcccaagatc
    10981 ttttaaagcc gttagtgtct ctttctttat attgctgtca gtaactattt tagagataca
    11041 atctaactta cacactctaa aaaaagctac ttcctcaaat tttctacttt ccgccactaa
    11101 tatgatctct tgagcagatt caatcataac tttttttaag tgagcttcaa gctcaaaagt
    11161 atcagcaata tatcctttgt ccactgaaat agctttggtt cctataaaaa gtttatcagc
    11221 atgataatta agcactgcat tttctgcttg agggcctaca aaagaaaaag agggaggatg
    11281 taaagttcca ccggtagaga taatgttgaa ttttcggttc tgagccaaat ttatacagat
    11341 atcaagggaa ctggtaagaa tggtaatttg taatccaggg ctgatgtttc ttgctacctg
    11401 taaagcagta gtgctggcat caaaaaccac tgagtctcca tcttctataa gcttagctgc
    11461 tttcttacca attaattcct tgatttcccg gttagttatc tctcgaagag taaaaggaag
    11521 ctctactact cctcttttta tcctggtagc cccaccataa attcttcgca ataatctctg
    11581 ctcttctaaa atttttaggt cctgacgaat agtatcttcg ctcactccaa aaacatcact
    11641 tagttcctta accgttatgg ttctcttttc atctagcaat tctaatatct ttaatcgcct
    11701 ttcctctgaa aacatactaa attcacctaa cttctttctt attttaatac caaattactt
    11761 gcaatttttt ccagaatact atatagttta atcaatgtca atagcaattg agctaccaac
    11821 caataatctc tacatctacc aaaatcaaat atagttaacc gcaaaatcca ttataaagac
    11881 tatgggggta aaccccctca taaccccgaa ctataaaggg gggaacccct ttatagttcc
    11941 ccaaaaaaca taaaacctaa caaacaagaa caattgaggg agaaagagtc tccctcagtg
    12001 ggcagcgccc ctttataacc tcagcattac ccataagttt tttctggaca ccatagaagg
    12061 ttttcaa
//
LOCUS       DAOSVE010000051        11765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_3109_length_11765_cov_69.029035, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000051 DAOSVE010000000
VERSION     DAOSVE010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 11765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11765
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3109_length_11765_cov_69.029035"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PK016_05505"
     CDS             <1..313
                     /locus_tag="PK016_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HXL02558.1"
                     /translation="ALKVALKVKSGDCERGIMLDGAGIGSSMVCNKIRGIRTALCYDL
                     KTAVNSRKHNNTNVLTLGGPLHPIEELYEMVKVWLETKFAGGRHWKRINKMMDIEKKY
                     L"
     gene            487..1146
                     /gene="deoC"
                     /locus_tag="PK016_05510"
     CDS             487..1146
                     /gene="deoC"
                     /locus_tag="PK016_05510"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HXL02559.1"
                     /translation="MTPAGLASYIDHTLLKPDATQAQIEQLCQEAIQYSFCSVCVQPF
                     WVSYCARKLRGTKVKVCTVVGFPHGANVPPTKAFETRQAIEDGAQEIDMVINVGALKS
                     GNLKVVEEDLRAVKRACHSTTITKAIIEAFLLTDDEKITVCQLAKKVGYDFVKTSTGF
                     AGGGATVHDVALMRRVIGPKMGIKAAGGIRSFEDAKAMVLAGATRLGTSASIKIVTGQ
                     N"
     gene            1161..1835
                     /gene="pduL"
                     /locus_tag="PK016_05515"
     CDS             1161..1835
                     /gene="pduL"
                     /locus_tag="PK016_05515"
                     /EC_number="2.3.1.222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HXL02560.1"
                     /translation="MSESGLISDQMLSEIISEVVYKYVVEGESILEGEYSIPVEISRR
                     HVHLTRAALDTLYGKNYQLTKVRDISQPGEFASEEKVIIISPRMKVIEGVRVVGPLRE
                     YTQIELAMTDAFLLGLDSIPTRLSGDIAGSQPITLVGPKGSLTLSEGAIRAARHVHMT
                     PRDAEKYRVKNGDRVRIETRGRNGVIFKDVIVRVSDKSKLAFHIDTDEANAANIKGLT
                     FSRILY"
     gene            1955..2731
                     /locus_tag="PK016_05520"
     CDS             1955..2731
                     /locus_tag="PK016_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HXL02561.1"
                     /translation="MKKEERINYIANILSLQGIVRIRELSLKLGVSEMTVRRDLDSLA
                     QANIADLIPGGAVLKRNSSSDLGEEHYFFSAAGSQMIQEKIRVCRKAASLIEANDTIA
                     IDVGSTTEQLPQFIPSSLPLTIICFTLNILFKVYGNENWEITLTGGRFHRNTLMFESP
                     EGVELIKKMRINKAFISAAGVDEKLGVTCANHYEVETKKAAMKSSDTKILLSDSSKFG
                     KVKIAYFADLSDFDLIITDSNLDPYYQDVIRGLGLQLILV"
     gene            2969..4225
                     /locus_tag="PK016_05525"
     CDS             2969..4225
                     /locus_tag="PK016_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HXL02562.1"
                     /translation="MGKTMAEKILENKVGFPVKAGDLVIARVDRAMGQDGTSPLAIQS
                     FEEMKGEKVFDPEKIILVLDHNAPSPLESVSRLHDLMRNFSRKFGVQLMEIGEGVCHE
                     LMVSRGLVVPGDLVVGADSHTCTYGAIGAFSTGVGSTELAVVMLSGELWFKVPETIRL
                     NLKGKLPAGVFSKDVILYLAGKLGADGATYQALEFHGPVIENLEVEERFTISNMAVEV
                     GAKAGLMVPDEKAIKWVRERTNREFQPVFPDSDAQYSQIIEVDCSSLSPQVALPHQVD
                     NVVPIEEIGEVPIQEAFLGTCTNGRLQDIGVAARILEGRKVHPGVRLIVAPASREIFI
                     EAIKRGWVEKIVLAGGVFVTPGCGPCVGTHQGVPGDGWNVISTANRNFKGRMGNARSF
                     IYLASPATVAASALEGKITDPRKYLR"
     gene            4232..4738
                     /locus_tag="PK016_05530"
     CDS             4232..4738
                     /locus_tag="PK016_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HXL02563.1"
                     /translation="MILRGRAHKFGDNINTDYIISGKYKFKTLDMNELAKHVMEDVDP
                     HFYEKIKPGDFIVAGRNFGCGSSREQAPRALSAAGIRGVLAQSFARIFFRNAINCGLA
                     ALECDTSPIEDGDELEVDLLTGKVVDHSRGIEIKTQPLPPVMVDILKEGGLIEYFKKY
                     GSFQGVKR"
     gene            4742..5998
                     /gene="glyA"
                     /locus_tag="PK016_05535"
     CDS             4742..5998
                     /gene="glyA"
                     /locus_tag="PK016_05535"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HXL02564.1"
                     /translation="MWELVKEVDPEVYHWIIEELERERNNLELIASENFTSLAVMQAQ
                     SSILTNKYAEGYPRRRYYGGCEFVDEVESLAIERAKELFGAEHANVQPHSGSQANMAV
                     YFAVLKPNDTVMGMDLSHGGHLTHGSRVNFSGILYNFVPYGVDPSTETIDYAALEKRA
                     KEVKPRLIVAGASAYPRFIDFERMRHICDEVGAYFMVDMAHIAGLVAAGVHPSPIPCA
                     HFVTTTTHKTLRGPRGGMIFCQKEFASAIDRAIFPGTQGGPLLHVIAAKAVALREAST
                     PEFVDYQKQVVKNAKVLGESLSARGYRLVSGGTDNHLLLVDLREVRITGKEAEKVLDE
                     VGITVNKNAIPFDEEKPTVTSGIRIGTPACTTRGMKEKEMEIIAECIDKALRGKNDPQ
                     ILNQVKNQVRELCQRFPLYPEIANGE"
     gene            6052..6273
                     /locus_tag="PK016_05540"
     CDS             6052..6273
                     /locus_tag="PK016_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HXL02565.1"
                     /translation="MREKVEKALEKVRGYLQFEGGDVELVDIEDGVVKVRLKGMCSAC
                     PMAMFTLKDGIEQAIKEEVPEVKSVEQVL"
     gene            7329..>8034
                     /gene="deoC"
                     /locus_tag="PK016_05545"
     CDS             7329..>8034
                     /gene="deoC"
                     /locus_tag="PK016_05545"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HXL02566.1"
                     /translation="MRIEEIREWRKRVEDHLGMEIKEKKEFPPESIPQEEEELRKFLA
                     HHIDCTILNPHATLKEVEDVCREAKEWGFWEVIVNPFYVPTCAQFLEGTKIMVGVAVG
                     FPLGQNKPEIKAREARLAFQEGAREADMVINLGALKNGDWKLVYQDIRGVVEEMSPRI
                     IKVVLENCYLSTEEKVVGCFIAQAAGAHFVKTSTGFGSGGATVEDVELMRQVVGESMG
                     VKAAGGIHSREIALQMI"
     assembly_gap    8035..8134
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8135..8455)
                     /locus_tag="PK016_05550"
     CDS             complement(<8135..8455)
                     /locus_tag="PK016_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXL02567.1"
                     /translation="MFRGWQKVSYIEYPGNIATVLFTAGCNFRCPYCHNSELVESPQI
                     FPEVKEEEVLRYLEERKGLIDAVCITGGEPLLWKEELLLFLTKVKEQGFKVKVDTNGS
                     SYSSF"
     gene            complement(8461..10545)
                     /locus_tag="PK016_05555"
     CDS             complement(8461..10545)
                     /locus_tag="PK016_05555"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HXL02568.1"
                     /translation="MKEKIELSPKEVRKRDGRVVPFDRQRIERAIYKAFQAVGEENTV
                     IPEELSHTVTEKLFYKFGTDTTVDIEAIQDVVEETLIENGFAKVAKAYILYRRKRQEA
                     REALQVAVNVEKIVQDYLIQVDWRTQENSNTTYSYPGLVLHVAGSVMAHYTLNQIYPD
                     EVKEAHVRGDIHIHDLSYGITAYCAGWSLEELIREGFGGVKDKIAAGPARHLSALTGQ
                     MVNFLGTMQMEFAGAQAFNSVDTYLAPFVRYDHLDYRATKQLIQQMVFAMNVPSRWGS
                     QAPFLNFSFDWVAPEDLKDRPVIIGGEERPDLGTYGEYQEEMNMVNRAFIEVMLEGDY
                     EGRVFSFPIPTYNITSDFPWDSENARLLWELTAKYGVPYFQNFISSSLKPGDVRSMCC
                     RLQLDLRTLRNRCGGLFGSADKTGSIGVVTINLPRLAFLSKTEEEFFARLDRVMNIAK
                     TALEIKRKVVERNLKNGLLPYTRRYLGTFRNHFSTIGLVGMNEACLNFLGVSIAEKEG
                     KEFAVKVLNFMRNKLADFQEETGNLYNLEATPAEGASYRLARHDHRDFPHIITAGEAE
                     PFYTNSTNLPVNYTDDPFEALEHQKDLQVLYTGGTVFHAFLSEMPHPEAVKLFIKRAF
                     ERYPIPYLTITPTFSVCPNHGYIRGKKFNCPECGAETEVYSRVVGYYRPIQRWNKGKQ
                     EEFKERKEYALS"
     gene            complement(10529..11023)
                     /gene="nrdR"
                     /locus_tag="PK016_05560"
     CDS             complement(10529..11023)
                     /gene="nrdR"
                     /locus_tag="PK016_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HXL02569.1"
                     /translation="MHCPYCGEEDSRVIDSRIVEGGAAVRRRRECPRCQRRFTTYERY
                     EQEPLRVIKKSGERELFNRQKILQGMLKACEKRPVTMEVLESLAGEIEKELRDEGYEE
                     IPTSRIGEKVMEKLREVDQVAYVRFASVYREFREIDQFLELLSTLNREKNTSERREVN
                     ERKD"
BASE COUNT         3404 a         2135 c         2645 g         3481 t
ORIGIN      
        1 tgctttaaaa gtagctttga aagttaagtc tggtgattgt gaaaggggaa ttatgctcga
       61 tggagcaggg ataggttctt ccatggtatg caataaaatc aggggaatac ggacggcact
      121 ttgttatgac ctcaaaactg ctgtaaatag taggaaacat aataatacta atgttcttac
      181 tcttggtggt ccattgcatc cgattgaaga attatatgaa atggttaaag tttggttaga
      241 gacaaagttt gctggtggaa ggcattggaa acggatcaac aaaatgatgg atattgaaaa
      301 aaaatattta taaaattaat gttagaagcc tattatggat caagaaaatc ttattgataa
      361 aattactcaa aaagtgatga atagaataga ctgagtggga tgggatatag tgaaaggggg
      421 tctaccgttg tgagtcaaaa agccagaagt gtagttaccc aacaggagca tgggagttgc
      481 gaatgcatta ccccggcggg tttagcatcg tatattgacc acaccttgtt gaagcctgat
      541 gctactcaag ctcagattga gcagctctgt caagaagcta ttcaatattc tttttgttcg
      601 gtttgtgtgc agcctttttg ggtgagctat tgtgccagaa aattgagagg aacaaaagtt
      661 aaagtatgta cggttgtggg gtttccacac ggggctaatg ttccacctac gaaagcattt
      721 gagactcggc aagctattga agatggtgcg caggaaatcg atatggtaat caatgtcggt
      781 gcgcttaaat caggtaatct taaggtagtg gaagaggacc tgcgagccgt taagagagct
      841 tgccactcta caacaattac taaagcgatt attgaagctt ttcttttaac cgatgatgaa
      901 aaaattactg tttgtcagtt agctaaaaag gtgggatatg actttgtaaa gacttccact
      961 ggctttgctg gcggaggagc tactgttcat gatgtggctt taatgaggag agtaattggc
     1021 cctaagatgg gaataaaagc cgcaggaggc attcgtagct ttgaagatgc aaaagctatg
     1081 gtgttagctg gtgctacgag gcttggaact agtgctagta taaaaattgt aactgggcaa
     1141 aattaaaagg gaagaaagtc atgagtgaaa gcgggcttat aagcgaccaa atgttatcgg
     1201 agattataag tgaggttgtt tataaatatg ttgtggaggg tgagtctatt ctcgaggggg
     1261 aatattctat accagttgaa atttctcgtc gacatgttca cctgacccga gctgctttag
     1321 atactttata cggaaaaaat taccaattga ctaaagttcg agatatatca caacctggtg
     1381 aatttgctag cgaggagaaa gtaattataa ttagccccag gatgaaggtt attgaagggg
     1441 tgcgtgttgt aggcccatta agagaatata ctcagataga gttagcaatg accgatgcct
     1501 ttcttttggg tttggattct attcctactc gtttgtcggg agacatagct ggttcccaac
     1561 cgattactct ggttgggcca aaaggttcct taactcttag cgaaggagct atacgtgctg
     1621 cccgacacgt tcatatgacc cctcgggatg cagaaaaata tagagttaaa aatggcgatc
     1681 gagtaaggat tgagactaga ggaagaaatg gagtaatctt caaagatgtt atcgttagag
     1741 tttctgataa atctaagtta gcttttcata ttgacactga tgaggctaat gctgctaata
     1801 ttaagggatt aactttctct agaattttgt attgataaca gtctctggcg aaaacgagca
     1861 gcatcctaat aaatatatac tcacagtaag cggatttctt aaatgcagtg attatagtat
     1921 aatttagtga ttaaatttgt tttttggaga ttagatgaaa aaagaagaaa gaattaacta
     1981 tattgctaat attctttccc tgcaagggat tgtccgtatc cgggagcttt ccctaaaatt
     2041 aggggtttca gaaatgactg ttcgccggga tctagactcg ctagcacaag caaatattgc
     2101 tgaccttata ccagggggag cagtgcttaa gcggaactcc tcttcggatt tgggagaaga
     2161 acattatttc ttttctgctg ccgggtctca gatgattcag gaaaaaataa gagtttgccg
     2221 gaaagctgct tctttaatag aagccaatga tactattgcc atagatgtcg gcagtactac
     2281 tgaacaatta ccgcagttta ttccctcttc ccttcctctt actattattt gttttaccct
     2341 taacatcctg ttcaaagtgt atggaaatga aaactgggag attacactta cggggggccg
     2401 ttttcacagg aataccctga tgtttgaaag tccagaaggg gttgagctta taaaaaaaat
     2461 gcgaattaat aaggctttta tttcagcagc aggtgttgat gaaaaattag gggtaacttg
     2521 tgctaaccat tacgaggtgg aaaccaaaaa agctgcgatg aaatcctcag acaccaagat
     2581 tttactctcg gattcttcca aatttggtaa agtcaagatt gcctattttg ctgacctttc
     2641 agatttcgac ttaatcatta ctgattctaa cctggatcct tattatcaag atgtaatacg
     2701 gggtttgggg ttacagctaa ttcttgtgta aattcaggcc gctctctggg aattaaaccc
     2761 atcttcaaag tttctacatt tgtgaactct acattgcagg agatatcctc atcttttatt
     2821 ttcttggtgt ggggttagct attgttgtcc caaccgggcg tcgcaaatgc agtcatgtcc
     2881 tatctcaggt aattcatccc attgcttttt taaaaacctt actgtattat gagaaaatgt
     2941 aaaaaagggt tagataagga gcgatactat gggaaaaact atggcagaga aaatactgga
     3001 gaataaagtt ggttttccgg ttaaagcagg agacctggtt atagctcggg tagatagggc
     3061 tatggggcaa gatgggacta gtcccttagc tatccaatct tttgaagaga tgaagggaga
     3121 gaaagttttt gacccggaga aaataatttt ggttttagac cacaatgctc ccagtccttt
     3181 agagtcagtt tctcggcttc atgacttgat gcgtaatttt tcccggaaat ttggagtgca
     3241 gcttatggaa ataggagaag gagtatgtca tgagttgatg gtatcccggg ggttggtggt
     3301 gccaggagat ttagtggtgg gagctgattc ccatacttgc acttatggag cgattggtgc
     3361 tttttctacc ggggtgggaa gtacagagtt agcagtagtg atgctctcgg gagaattgtg
     3421 gtttaaagtt ccagaaacta ttcgcttaaa cttgaaaggt aaattaccgg caggggtatt
     3481 ttctaaagac gttattcttt atctggctgg taaattagga gctgatggag ctacttatca
     3541 ggctctggag tttcacggac cggtgataga aaatttagaa gtagaagaac gctttaccat
     3601 ttctaatatg gccgtggaag tgggagcgaa agctggtctt atggttcccg atgaaaaagc
     3661 gataaaatgg gtgagagaga ggactaatcg ggagtttcag ccagttttcc cggactctga
     3721 tgctcaatat tctcagatta tagaggtaga ctgttcttct ctttctcctc aagtggctct
     3781 tcctcaccag gtagataatg tagtgcccat agaagaaata ggagaagtcc ccattcagga
     3841 agcttttttg ggaacctgca ctaatggaag gctacaagat ataggagtgg ctgctcgtat
     3901 tttagaggga agaaaagttc atccgggggt gcgattaatt gttgctcctg cctctcgaga
     3961 gattttcata gaagcaataa aaagaggatg ggtagagaaa attgtccttg ctggaggagt
     4021 ttttgttacc ccggggtgtg gcccttgtgt gggaactcat cagggggtgc caggagatgg
     4081 ctggaatgtt atttctacgg ctaaccgtaa ttttaaagga agaatgggca acgctcggtc
     4141 tttcatttat ttagcttctc cagctacagt ggcggcttcg gccttagagg gtaaaattac
     4201 cgatcctaga aaatatttga ggtgagagaa aatgattttg cgaggtagag ctcataagtt
     4261 tggagataac attaatactg attatattat ctccggaaaa tacaaattta agaccttaga
     4321 tatgaatgaa ctggctaaac acgtgatgga agatgttgac cctcattttt atgagaaaat
     4381 aaagccgggc gattttatag tggcggggag aaattttggt tgtggttcat cccgggagca
     4441 ggctcctcga gcgctctctg cggcaggaat ccggggggtt ctcgctcagt cttttgcccg
     4501 tattttcttc cgcaatgcta ttaattgtgg tttggcggct ttagaatgtg atacctcccc
     4561 aatagaagat ggagatgaat tagaagttga ccttcttacc ggaaaagtgg tagaccactc
     4621 tcgaggaata gagattaaaa cccaacctct acctccggtt atggtggata tcctgaaaga
     4681 agggggctta atagagtatt ttaaaaaata cggttctttt cagggggtca aaagataggc
     4741 tatgtgggag ctagtaaaag aagtagatcc ggaagtttat cactggataa tagaagaatt
     4801 agagcgagag agaaataatt tagaattaat tgcttcggag aattttacca gtttagcagt
     4861 gatgcaagct caaagctcta ttttaaccaa taaatatgct gaaggatatc ctcgccggcg
     4921 ctactatggg ggttgtgaat ttgtagatga agtggagagc cttgctatag agagagccaa
     4981 agagcttttt ggagcagaac atgccaatgt tcaaccccat tctggttctc aagctaatat
     5041 ggcagtttat tttgccgttc tcaagcctaa tgatacagtg atgggaatgg atttgtctca
     5101 tggaggacac ctcacccatg gaagccgggt aaatttctcc gggatattat acaattttgt
     5161 gccttatgga gtagaccctt ccactgaaac catagattat gcggctttag aaaaacgggc
     5221 aaaggaagtt aagcctcgtt taatagtggc gggagccagt gcttaccctc gtttcattga
     5281 ttttgagagg atgcgccata tttgtgatga ggtaggcgct tattttatgg tggatatggc
     5341 tcatattgcc ggattggtag ccgcaggagt tcatcccagt cctattcctt gcgctcattt
     5401 tgttaccact accactcaca agactcttcg aggtcctcgg ggaggaatga ttttttgtca
     5461 gaaagaattt gcttccgcta tcgaccgggc tatatttccc gggacgcagg gaggtcctct
     5521 tcttcatgtc attgctgcta aagcagtagc cctccgggaa gcttcgactc ctgagtttgt
     5581 ggattaccaa aaacaagtag tgaaaaatgc taaagttttg ggagaaagtc tgagcgctcg
     5641 gggataccga ctggtttctg gtggaactga taaccatctc ctcttggtgg atttgcggga
     5701 ggtgagaata acgggaaaag aagctgaaaa agttttagat gaagtgggaa taacagtgaa
     5761 caaaaatgcc attccttttg atgaggaaaa gcccaccgta accagtggta tcagaatcgg
     5821 cactcctgct tgtaccacgc gaggtatgaa agagaaagaa atggaaatca tcgctgagtg
     5881 catagataag gccctccggg gaaaaaacga tccccagatt ctcaatcagg ttaagaatca
     5941 ggtgagagag ctatgtcaga gatttcctct gtatcccgaa atagccaatg gggaataaaa
     6001 tatagaaatg tggtttacta ttaggatact atacttagga ggtaagaaat gatgagagaa
     6061 aaagttgaaa aagctttaga aaaagtaaga ggttatctcc agtttgaagg tggagatgta
     6121 gagctcgttg atatagaaga tggagtggta aaagttaggc tgaaaggtat gtgcagtgcc
     6181 tgccctatgg ctatgtttac tcttaaagat ggtattgaac aggccataaa agaagaggtg
     6241 cccgaggtaa aatcagtaga acaagtacta tagcagcctg cggtgtaaga taaaagcata
     6301 agtttaggaa acgcttctca atatagcatg atggtatctt ttattaaaaa agataccatc
     6361 atgctatatg aaaagacaaa gccttgccaa tgatatagga actccactta taaattctcg
     6421 ggaagaataa taacctttct ctttgtgttc tatagttgac tgcaaaaact cgtggtaatg
     6481 tccatctact tagtaattta cttctctcta aggggtcaat tgcaagtatc gaaaaagacg
     6541 ttttttgtta ttcctggaat ctttaatcag gcatactcct cttctatcat tcccgaaatt
     6601 ttcaatcagg aatctaaaga tttttctggg gatggatttt tatccccagt agtttttact
     6661 ttttaatctt ttgtgttttt tttgctttcc aggggtaata aggggttcac cccattgagg
     6721 aaagttcttc cttcctcagt ggtctttatg ttttcctgtt tttctgtgat ttttttcagg
     6781 aggtaatagg gaaatttgtc tctctatagt tttagacccc tgatagaaac actcaggttc
     6841 caatttaaca gattttgcag gaatgctata tacttactaa cccaatttgc atcataaatt
     6901 ccagaaatca gcctatatca gaactttaat tttgggaatt ctcattttcg ggcagagatt
     6961 tgcaactgag gttggttttt tgtcttttag tttacaccat agatagtctc agatgcccga
     7021 ttactaattt cggacatgac agaaaaagaa aaacagtagg cactaaggaa gtgtttaccc
     7081 ttcctcggtt gtatttgttt ttttaatgtt gttttgcttt tccgggggtt ataagggggt
     7141 ttaccccttt attgttgttt acaggataat tgtgttataa gagcagggag aattttctgg
     7201 taaaaatttt ataactcggg ttaaaatagt ctgctccttt ttagttttct agggtttatc
     7261 atttacaaag aagaaaaagg gaaatagaat aaacaactgg taacaaatgg tttaaaaagg
     7321 agataaaaat gaggatagaa gaaattaggg agtggagaaa aagggtggag gaccatttag
     7381 ggatggagat taaagagaaa aaagaatttc ccccggaaag cattcctcag gaagaagaag
     7441 agctgcggaa atttttagct caccacattg actgcaccat acttaatccc cacgctacgt
     7501 taaaagaggt agaagatgtg tgtagggaag ctaaagagtg gggtttttgg gaagtaattg
     7561 ttaatccttt ttatgttcct acctgtgctc agtttcttga aggcaccaaa ataatggtgg
     7621 gagtggcagt gggttttcct ctgggacaga ataagccaga aataaaagct cgagaagctc
     7681 gattagcttt ccaggaggga gcgagagaag cagatatggt aataaactta ggagcgctca
     7741 aaaatggcga ttggaagtta gtttatcagg atatccgggg agtagtggag gaaatgtctc
     7801 cgaggataat aaaagtggtt ctggaaaatt gctatctatc tacggaagaa aaagtggtag
     7861 gatgttttat tgctcaagct gccggagctc attttgtaaa aacttctact ggctttggtt
     7921 ctggtggtgc cacagttgaa gacgtggagc ttatgcgaca ggtggtgggt gagagtatgg
     7981 gagtaaaagc agcaggtggt atccactccc gggaaatagc tttgcagatg attannnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaaaaga agagtagctg cttccattag
     8161 tgtccacttt aaccttaaat ccttgctctt taactttagt aaggaaaagg aggagctcct
     8221 ccttccacag aagcggttct cctcccgtaa tacacactgc atctattaaa ccttttctct
     8281 cttctaaata ccgaagtact tcttcttcct ttacctccgg gaaaatctga gggctctcca
     8341 ctaattccga attatggcag taaggacagc gaaaattgca tccggcagtg aaaagcacgg
     8401 tagcgatgtt ccccggatat tctatatagc ttactttctg ccagcctcga aacattggtt
     8461 ttaagaaagc gcgtactctt tgcgctcttt gaactcttct tgttttcctt tattccacct
     8521 ttgaatgggt cgataataac ccactactcg agaataaact tctgtttctg ctccacattc
     8581 cggacagttg aatttcttac cccttatata tccatgattg ggacagacag aaaaagtagg
     8641 agtgatggta agataaggaa tgggataacg ctcaaaagct ctcttaatga ataactttac
     8701 cgcttcaggg tggggcattt cagagagaaa agcgtgaaat accgtccctc cagtgtataa
     8761 gacttgtaaa tctttttgat gctctaaagc ttcgaagggg tcatcagtat aattaacagg
     8821 taagttagtg ctattggtat agaaaggttc tgcttctcct gcagttataa tatgaggaaa
     8881 atcccggtgg tcatgtcgag ctaagcggta actggcccct tctgccggag tggcttctaa
     8941 gttgtaaagg ttgccagttt cttcttgaaa atcggctaat ttgtttctca taaaattcaa
     9001 caccttgaca gcaaactctt ttccttcttt ctcagcaata gatactccta aaaagttgag
     9061 acaagcttca ttcatcccca ctaaacctat ggtagaaaaa tgattacgaa aagtccctaa
     9121 ataacgacgg gtataaggca aaagtccatt tttcagattt ctttccacta cttttctttt
     9181 tatttctaaa gcagttttag cgatgttcat caccctatcc agacgagcaa aaaattcctc
     9241 ttcagtttta cttaagaaag caagacgagg aagattaata gttacgacac caatagaacc
     9301 agttttatcc gcagagccaa aaagccctcc acaacgattg cgcaaagttc gcagatccag
     9361 ctgcagccgg caacacatag aacggacatc accaggtttt aaggagctag aaataaagtt
     9421 ttgaaaataa ggaacaccat atttagcagt aagctcccag agcaatcggg cattttctga
     9481 gtcccaggga aaatcagaag taatattata agtagggata ggaaaagaaa acactcttcc
     9541 ttcgtaatct ccctctaaca tcacttcgat aaaagctcga ttgaccatat tcatttcttc
     9601 ctgatattca ccataagttc ccagatccgg tctttcttcc cctccaatta tcactggacg
     9661 gtcttttaag tcttcagggg ctacccaatc gaaagaaaaa ttcaggaaag gagcctggct
     9721 tccccaacga gaaggaacat tcatagcaaa caccatctgc tgaataagtt gtttggtagc
     9781 tcgataatca agatggtcgt agcgcacaaa aggagcaaga taagtatcta cagaattaaa
     9841 agcctgagca ccagcaaact ccatctgcat agtgcctaaa aagttaacca tctgtccagt
     9901 gagagcagaa agatgccggg ctggaccggc ggctatttta tcttttactc ctccaaaccc
     9961 ctctcggata agctcttcta gcgaccaccc ggcgcaataa gcagttattc cataagagag
    10021 gtcgtgaata tgaatatcgc cacgcacatg agcctctttt acttcatcgg gatatatctg
    10081 gttcaaagta tagtgagcca taactgagcc agctacgtgg agaactagcc cggggtaaga
    10141 ataagtggtg ttagaatttt cttgcgttct ccaatccacc tggataagat aatcctgaac
    10201 aattttttct acatttactg ctacctgaag agcttctctg gcttcttgtc ttttgcgccg
    10261 ataaagaata taggccttag ctactttggc aaaaccattt tcaatcagag tttcttctac
    10321 aacatcctgg atagcctcaa tatctacagt agtatcagtg ccaaacttat aaaacagttt
    10381 ttcagtaacg gtatgactta actcctcagg aatgacagta ttttcctctc ccacagcttg
    10441 aaaggctttg taaatggctc gctcaatcct ttgcctatca aaggggacta ccctcccgtc
    10501 ccttttccgt acttctttgg gtgataattc aatcttttct ttcattaacc tctctccttt
    10561 ccgaggtatt tttttctcga ttcaaagtag aaagcaattc taaaaactgg tcaatctctc
    10621 gaaactctcg atagaccgag gcaaaacgaa cataagctac ttggtctact tcccttagct
    10681 tctccataac tttttctcca atacgggaag taggaatctc ttcgtaacct tcatcccgta
    10741 gctctttttc tatttccccg gctaaagact ctaaaacttc catagttacc ggtctttttt
    10801 cgcaagcttt gagcatccct tgtaaaattt tttgccgatt aaataactct ctttccccgc
    10861 tctttttgat tactcgaaga ggctcttgct catagcgctc ataagtagtg aatcttcgct
    10921 ggcagcgggg acactctctt ctgcggcgta ctgccgcccc accttccact attcgggaat
    10981 caataactcg agaatcttct tctccacaat aaggacaatg cataatttac ccaatatatt
    11041 gaaatattat tactaataac cactacatat agtatacagg tttttatttt aattgcaaga
    11101 ggtaaattga aagttgagga aaatattttt tgttgaaggt tggaaagcca atttgtagta
    11161 aagctgcaaa agcaattata acgttcctct atttggtgtt ctatttttct tcttgagtat
    11221 cagcaatact cgcctgatgt ctcttttcca tcatgtcttt agccaggcat tcttctcatt
    11281 tgtcatttcc gaaattttca atcggaaatc caaaagacta ctgggaaatt ttttctcccc
    11341 agtggtcttt tttgtctttt tcggaggatt atgagagagg gggtgtttct cctttattgt
    11401 tctgcgccgc aggctgttct atttttttaa ggggattata agtgagagaa tgccccctta
    11461 tagtttagat ccctcgtaga accattcagg ggtgacggac ttgggggaaa agacgctttc
    11521 actttgtatt ttttctccct ccagggagag gggaaaagaa aaggaatcga gggcttctct
    11581 tttttgtcat tcccgaaatt ttcaatcgga aatccaaaag tttttactga ggagagaagt
    11641 tccctcagtg attttaatct ttttagtccc tttttttgtt cctacgtcgt aggctatttt
    11701 gtttttttgc aggcactata aatagcttaa acgggggaag atgaccaccc caaaccgagc
    11761 gttca
//
LOCUS       DAOSVE010000052        11626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_3146_length_11626_cov_75.461239, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000052 DAOSVE010000000
VERSION     DAOSVE010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 11626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11626
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3146_length_11626_cov_75.461239"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..1375)
                     /gene="melA"
                     /locus_tag="PK016_05565"
     CDS             complement(62..1375)
                     /gene="melA"
                     /locus_tag="PK016_05565"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HXL02570.1"
                     /translation="MKITFIGAGSLVFTQRLLADLVRLPFPKEEIEVGLVDIDEKRLS
                     YINRITQRIFSENGISTDKIISSTDRREVLPGSDYVFISILVGGVEVIRQDIEIPLKY
                     GVDQCIGDTIGPGGIFRAMRTVPVILDIAEDIEELCPEAVVLNYTNPMSIICWVAKEL
                     YPLKFYGLCHSVQNTARELSTYLGIPRKEIYYWVAGINHQAWFLELSRNGEDLYPLLA
                     EKAQDPEIVKKDTTRMEMFKHLGYYVTESSGHNSEYNPWFRKRKDLLEKYTPGGGWNG
                     ETGFILKLYGGDREKYEEELEKIASGKEPIKYEESEEYGMKIIYALESGEVFRANINL
                     PNGGLITNLPPQCVVEVPCFVEKGRIRPALVGELPIQLAALNQMVVQSQEMAVRGILE
                     EDRDMIYYSLYYDPLTAAVLSLEEIKRMTDEMFEAEKDYLPGWQE"
     gene            complement(1771..3087)
                     /locus_tag="PK016_05570"
     CDS             complement(1771..3087)
                     /locus_tag="PK016_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HXL02571.1"
                     /translation="MSKITIIGAGSGVFTRNLVRDILSYPELSGSTISLMDIDASRLE
                     YMRKALQRLVEQEQYPAKLEATLNRKEALKGADYVILTIQVGGLKPFEHDIYIPLKYG
                     VRQAVGDTIGPGGVFRALRTIPVILDIAKDMEELCPEALLLNYVNPMAMNCWALNQET
                     NIKNVGLCHSVQGTAEFLAGIIGVSMEDISYLCAGINHMAWFLRFEANGKDAYPIIRE
                     KAKDPEIYTQDVTKFEVLRHFGYYVTESSFHLSEYVPYFRKSDDWINRIHRTHSWHKE
                     YYDGMYLHCCLDAAKTLLEDLREMAEASYIDPRRSREYCATIIHSLETNSPNVINGNV
                     ENKGLITNLPEGCCVEVPCLVNRNGIQPTFVGNLPSQLAALNRTNINVQELAVTGALA
                     ADREAVYHAVMMDPLTSAVLDLDEIRQMVDEMFEAEKEYLPEEWNK"
     gene            complement(3146..4213)
                     /locus_tag="PK016_05575"
     CDS             complement(3146..4213)
                     /locus_tag="PK016_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL02572.1"
                     /translation="MVEQENVLMQIADLCTYFYTDDGVVRAVDGVYLDIEKNKVLGII
                     GESGCGKTVLSLSMLRLVPPPGRVVRGNILYYMENGSCIDLAKLSPKGSTMREIRGMH
                     ISMIFQEPMVSLNPLYTIGEQIIENVVTHIQITKKEARELAIHMLEQVGFPEPAKGID
                     DYPHQLSGGMRQRAVIAMALVCNPKLIIADEPTTALDVTVQAQVLELLKDLQQRMGMA
                     VMIITHNLGVIAEIADYVGVMYLGKIMEYASTCDLFDQPLHPYTQALLKSVPYLAVQK
                     EGKRKLETIKGDIPDPYARIEGCLFASRCPHFQQDVCTQDPPLVEVENAHQVRCWLYS
                     QAGKILTEERAPRMGYKSNSL"
     gene            complement(4189..5220)
                     /locus_tag="PK016_05580"
     CDS             complement(4189..5220)
                     /locus_tag="PK016_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL02573.1"
                     /translation="MDNAILEVDNLGKYFPVTRGFWKEIVGHVKAVDKVSFYVEEGET
                     LGLVGESGCGKTTLGRCILRLIEPTEGKVWFKKNDGSKVELTKLQLKEFKEFRKEIRM
                     IFQDPYLSLDPRLTVMEIVGEPMKIHRMAKGKDLEDRVKNLLEMVGLKAQYMRRYPHE
                     FSGGQRQRIGIARALASNPRFVVADEPVSALDVSVQAQILDLLEKLQGELHLTYLFIS
                     HDLSVVQYVSDRVAVMYLGKLVELGTAEEIYNMPKHPYTEALMSAIPKVDPRRKIERI
                     LLKGDVPGPMHSPSGCKFHPRCHYADKVCSQEEPMLRDVGNHHYVACHFAEQLNLRGI
                     EAWLSKKMY"
     gene            complement(5242..6372)
                     /locus_tag="PK016_05585"
     CDS             complement(5242..6372)
                     /locus_tag="PK016_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL02574.1"
                     /translation="MIRKKKETKTVNIIEAEQGKSYLQLVWRRFKKSKMGLLGGIIIV
                     ILTILSVFAPFFSPHDYMEYDPEKAYCPPQRVRFVDADGKFHWRPFVYDLKLDVDPVT
                     YSRIYVEDTSKILYIRFFARGWNYKILGLHSNLHFIAVEEGSIHLLGTDRYGRDLLGR
                     IFLGGRISLTIALMGAIVTVILGSTLGIISGYYGGPIDTLVQRSIEILQSFPQLPLWM
                     ALSAAVPRTWTSLQVFVVMVMIFSFLNWPTLAREIRGKTLSLREQDFITAIKEMGGSD
                     SRIIFKHLFPNCLSHIIVVLTISIPELILMESTLSFLGLGIQPPMVSWGVLLQGAQRI
                     EVLGLYPWVMLPALFIVFAVLGFNFLGDGLRDAADPYSVKRS"
     gene            complement(6365..7360)
                     /locus_tag="PK016_05590"
     CDS             complement(6365..7360)
                     /locus_tag="PK016_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL02575.1"
                     /translation="MSRYVIQRLLMSIPVLIIISIVVFIVIQLPPGDYMSTVQAQLQS
                     LGGLTEDEAVKVAEQLRHSYGLDKPLYIQYFVWIKNIVTKGDFGFSFQYRKPVADVIW
                     ERIGWTLLIASLCHLVSTLVGILIGIYSATHQYSIGDNVATFFAFVGLSVPGFFLALI
                     AMYILAVKVGVEAGGLFSQRYILAPWGWGKFLDLLSHIWIPIVIVGFAGTARNMRVMR
                     GNLLDVLNLPYVLTARSKGLKEREVIFKHAVPNALHPLIMYQGMVLPFMIQGELIAST
                     VLNIPTAGPMFLNAINTQDMYLAGSFLLLLAVVLVIGNLLADIVLAWIDPRIRYD"
     gene            complement(7448..9457)
                     /locus_tag="PK016_05595"
     CDS             complement(7448..9457)
                     /locus_tag="PK016_05595"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL02576.1"
                     /translation="MKRKWSVLTLSIIVVVLVIVTSSLAQEIEIPKFEVGEIIQYKAL
                     PSYSEAPELAEAVKKGELPPVEERLPAKPAVVMTSGMSDGPGIYGDAWRTVSAVPTAG
                     WNWAAGVIAGWFGIEEAMQEALVNTGMSWRLKDGEPGPNLATHWEWSEDGKTLTMYLV
                     EGAKWSDGHPFTADDVLFTYYDCILDPNVPSWQGAGTWTLDGKVVELEKVDDYTIKWH
                     FAEAFPVYLLWYMDLLDFSVAPAHVLKPHHPKYNSEKTYEEFINALPPDDLPPVVLGA
                     WVPVYLKEDEALIMKRNPYYWKVDETGKQLPYIGEFYFKKARAGVQRDLDLIAGTCDQ
                     THLETPGLFTFINEQARLPGSPFKIVPGMWTLTFALEANLSLYQGVKNETDLAMRELL
                     RNLDFRKAISHAIDREAVARMLVPGPMLRALPSGFPSGADYYDPEAVVSYPYDPEKSK
                     ELLAGLEFKDTDGDGILNWTSGPLQGQNLTVAVKIHEDQTACVDIVEVLVPMFEEVGI
                     RLTPKIVKATVGQALDTAGEFELNMTRQDFLASPYTSLTSLAPVTPSSPNWHRAGPGE
                     KRDLLPFEERMAEIAEALKYERTREGRQELFSELQNLWTENLYTIGVIEGIYNTGITK
                     RFRNIPIGVPARFHQWFHLNVMQEQVWTPEEEQLPEFLPGVVVKY"
     gene            complement(9590..10609)
                     /locus_tag="PK016_05600"
     CDS             complement(9590..10609)
                     /locus_tag="PK016_05600"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HXL02577.1"
                     /translation="MKDKRITIKDVAREAGVSVATASRALGGYGYVTEETKEKVFNAA
                     LKLNYNYNALAKSLRTNKTMTIGYLLPDIANPFYSRIVRGIQDVAFEEGYNVIICSND
                     NDIIKTERFVKMFLRHRVEGIIYSTPYNQVLNDMCETFKKQRIPVINCYGSTRLCASD
                     IVIGDAEKGCYQAVHHLLELGHRQIAILRVKESGISKRRFEGCKKALEDFGAEINPHF
                     LVDAEDFSQESGYLQAKILFSYPQKPTAIVAFSEQLAIGVLEAAREDGIKVPEDMSLV
                     GVDDIIAEVLEPPLTSVALPTYEAGRTAASLLFSRLKDEESSPTRQVVLEEKLVVRKS
                     TRGRG"
BASE COUNT         3319 a         2768 c         2061 g         3478 t
ORIGIN      
        1 tcacgagtca cctcaaataa aataaagcaa aattgttatt ctgagaacat ttttaagtaa
       61 gctactcttg ccagcctggt aaatagtctt tttctgcttc aaacatctca tcagtcatgc
      121 gtttgatttc ctctaaggaa aggacagcag cagttaaagg gtcataataa agagaatagt
      181 aaatcatatc tctatcttcc tctaaaatcc ctctaactgc catctcctga ctttgaacca
      241 ccatttgatt taaggccgct agctgtatgg gtagttctcc caccaaggca ggtcttattc
      301 ttcctttttc tacaaaacag ggaacttcca caacacactg aggaggtaaa ttagttataa
      361 ggccaccatt gggtaagtta atattagccc ggaaaacttc tccgctctct agtgcatata
      421 ttattttcat tccatattct tcgctttctt catattttat aggctctttg ccactggcaa
      481 ttttctccag ttcttcttca tacttttctc ggtcacctcc atagagcttc aaaataaagc
      541 ctgtttctcc gttccatccc cctcccgggg tgtatttttc taaaaggtcc tttctttttc
      601 taaaccaggg attatactcg gaattgtggc cactggattc ggtaacataa taccctaagt
      661 gcttaaacat ctccattctg gtagtatctt ttttaactat ttccggatcc tgcgcttttt
      721 cagctaaaag gggataaagg tcttctccat ttctgcttaa ttctaaaaac caggcttgat
      781 gattaattcc tgctacccag taatatatct cttttctcgg tataccaaga taagtagata
      841 gctcacgagc agtattttga acactatggc aaagtccata aaacttcagg ggataaagct
      901 ctttggccac ccagcaaatt attgacatag ggttggtgta gttcaaaacc acagcttcag
      961 ggcagagctc ctcaatatcc tcagcgatat cgagaataac tggtacagtc ctcatcgctc
     1021 gaaatattcc ccccggacct atagtatccc cgatgcactg gtctactcca tattttaagg
     1081 ggatttctat atcctggcga atgacttcaa ctcctcccac caggatagaa ataaaaacat
     1141 aatctgaccc ggggagaact tctctgcggt cagtagagga aattatttta tcagtactga
     1201 tcccgttctc agaaaaaatc ctctgagtaa ttctattaat gtagcttaat ctcttttcgt
     1261 ctatatctac taaccccact tctatttctt ccttggggaa aggtagacga accagatcag
     1321 caagtaacct ctgagtgaaa acaagactcc ccgcaccaat aaaagttatc ttcacacttc
     1381 attacctcct gttttaattc tctagtagat atttactgcc actttcaagg ttctctatcc
     1441 tcgcttatac atatgtaaaa aagaggaaac atggccatat ttatagtcct acaaatttga
     1501 gaaatgctaa aactaacata tccacagaaa ataaaggttc ccaacggttt tatttttcca
     1561 aatatttcag tatcgaatct aacacttcct tctttcggta tgtattagct cgaaacaatc
     1621 aagtcacatt caccattgta atctctcaat tatacaggta atagcacgta ctattgagca
     1681 attcctcccc ggctttcaaa ttactaatta tcaatcacag aaaattatat ctatcttctt
     1741 cgattcatat ccatttaata ttctttgggc tcatttattc cattcctctg gtaaatactc
     1801 tttttctgct tcgaacatct catctaccat ctgtctgatt tcatctaagt ctaaaacagc
     1861 actcgttaat ggatccatca tgaccgcatg gtaaacagct tctctgtcag ctgcaagcgc
     1921 acctgttaca gcaagttctt gaacattgat attagtccga tttaatgccg caagctgtga
     1981 tggtaaattc ccgacaaagg taggctgaat cccatttcta tttacaagac agggtacctc
     2041 aacacagcat ccttcaggaa gattagtgat aagaccttta ttttctacgt tcccattaat
     2101 aacgttggga ctattcgtct ctagagagtg aatgattgtt gcacaatatt ctctgcttct
     2161 cctcggatca atataggaag cctcagccat ttcgcgcaaa tcctcaagaa gagttttggc
     2221 tgcgtccaag caacaatgta gatacatccc atcataatac tctttatgcc atgagtgtgt
     2281 tcggtgaatt ctattaatcc aatcatcgct ctttctaaag taaggaacat attctgaaag
     2341 atgaaagctc gactctgtca cataatatcc aaaatgtctt agtacctcga attttgttac
     2401 gtcctgggta taaatttctg gatccttagc tttttccctg attatgggat aagcatcttt
     2461 gccattggct tcaaacctca aaaaccacgc catatgattt atcccggcac agaggtaaga
     2521 aatatcttcc atagaaacac caattattcc agctagaaat tctgctgttc cctgaacgct
     2581 atgacatagc cccacgtttt ttatattcgt ctcttgattt agagcccagc aattcatagc
     2641 cataggattt acgtagttta gtagtaaggc ttcgggacaa agttcttcca tatctttggc
     2701 aatgtccaga ataaccggga tagttcgcaa agctcgaaat accccccccg ggccaatagt
     2761 atcacctact gcctgcctta ctccgtattt gagcggtata taaatatcgt gttcaaaagg
     2821 ttttaagcct ccaacctgga tggttaatat tacataatca gctcccttta atgcttcttt
     2881 tctattcaaa gtagcctcta atttagcagg atattgttcc tgttctacaa gccgttgcag
     2941 cgcctttctc atatattcaa ggcgactagc atcaatatcc atcaacgaaa ttgttgaacc
     3001 gcttaattct ggataagata aaatatctcg aaccaaattt cttgtaaaaa caccacttcc
     3061 tgcgccgata attgttatct tggacatgta ctatccctct cgctttcaat tatgtttcat
     3121 gttctttagc aactgaaaaa gaaaatcata aactattgct tttgtatccc attcgaggtg
     3181 ctctttcttc ggtcaaaatc tttcctgctt ggctgtaaag ccagcacctg acctgatgtg
     3241 cattctctac ttctactaaa ggaggatctt gggtacatac atcctgttga aaatgaggac
     3301 atctggaagc aaaaagacac ccctcaattc tagcatacgg gtctggaata tcacctttta
     3361 tggtttcaag ttttctttta ccctcttttt ggacggcaag ataaggtaca gattttaaca
     3421 aggcctgggt atatggatgg agaggctgat caaacaaatc acaagtactg gcatattcca
     3481 taatctttcc cagatacatt actcccacat aatcagcaat ttctgcaatt actcccagat
     3541 tgtgagtgat aatcatcaca gccataccca tcctctgctg gaggtctttc aaaagctcta
     3601 gtacctgagc ctgtactgtc acatccagag ctgttgtagg ttcatccgcg ataataagtt
     3661 ttgggttaca cactaacgcc atcgctatca cagcgcgttg tcgcatgcct ccactgagct
     3721 gatggggata atcatctata cctttggctg gctccggaaa tcctacctgt tctaacatat
     3781 gaattgcaag ctctcgggcc tccttttttg ttatctggat atgagtaacg acattttcaa
     3841 ttatctgttc cccaatagta tacaaaggat taagggatac cattggttct tgaaatatca
     3901 tcgatatatg cattcctctt atctctctca tagtgcttcc ttttggtgaa agcttagcca
     3961 agtcgataca acttccgttc tccatatagt aaagtatgtt tccccgcacc actctacctg
     4021 gaggaggaac aagccttaac atagaaagag aaagaaccgt cttaccacag cccgattcac
     4081 ctatgatgcc cagaaccttg tttttttcta tgtccagata aacaccatct acagccctca
     4141 ctacaccgtc atcagtatag aagtaggtac aaagatcggc aatctgcatt aatacatttt
     4201 cttgctcaac catgcttcta tccccctaag attaagctgc tctgcaaagt ggcaggccac
     4261 gtagtgatgg ttacctacat ctctaagcat tggttcctcc tgactacaaa ctttatcagc
     4321 gtaatgacac ctggggtgaa atttacaccc cgaaggcgag tgcatgggac caggaacgtc
     4381 tcctttcaac agtatacgtt ctatttttct tcgaggatct actttaggta ttgctgacat
     4441 taaagcttct gtatagggat gcttaggcat attatatatt tcttctgcag tacctaattc
     4501 tacaagtttt ccgagataca ttacggcaac tcggtcactc acatattgca ccacgctcaa
     4561 atcatgtgag atgaagagat aagtaagatg aagctctcct tgtaatttct caaggaggtc
     4621 cagaatctga gcctggactg agacatctag agcagaaaca ggttcgtcag ccacaacaaa
     4681 ccttggatta gaagccagag ctctcgcaat ccctattctt tgtctttgcc ctccactgaa
     4741 ctcatgggga tatctcctca tatattgagc tttcaagcct accatttcta gcaaattttt
     4801 taccctgtct tctaagtctt tgccttttgc catgcgatga atcttcatgg gttctcctac
     4861 aatttccatt actgtcagcc ttggatccag agataaatag ggatcctgaa atatcattct
     4921 tatctctttc ctaaactctt taaactcttt caactgcagc ttagttagtt ctaccttgct
     4981 tccatcgttt ttcttaaacc agaccttacc ctcagtgggt tctatcaacc ggagaatgca
     5041 tcttcctaga gttgtcttac cacatccgct ttccccaact aaccctaagg tttctccttc
     5101 ctcaacgtaa aagcttactt tatcaacggc ttttacgtgc cctactattt ctttccaaaa
     5161 acctcttgtc accggaaaat actttcccag gttatcaact tcaagaatcg cattatccat
     5221 ttgtgtattt tcccccaaac tctacgacct ttttacggaa taaggatcgg ctgcgtctct
     5281 cagcccatct cctaaaaagt tgaaccccag tactgcaaaa actatgaaga gagcaggcaa
     5341 cataacccat ggatacagac caagaacctc aatcctttga gctccttgta agagcacgcc
     5401 ccagctcacc atcggtggtt gaatgcccaa ccccagaaag cttaacgtgc tttccatcaa
     5461 aattagctca ggaatgctta tggtaagcac tacgattatg tggctgagac aattaggaaa
     5521 gaggtgtttg aagatgattc tcgaatctga tccacccatt tcttttatgg cagtgataaa
     5581 atcctgttct ctgagagaca gagtctttcc tcttatttct cgtgctaaag taggccagtt
     5641 caggaaggaa aatatcatta ccatcaccac aaagacctga agagaggtcc atgttctggg
     5701 aacggcagca cttaaggcca tccacaaagg cagctgagga aatgactgca atatctctat
     5761 agatctctga accagagtat ctattggacc accgtaataa ccagatataa ttccaagcgt
     5821 tgagcccagt ataactgtaa ctattgctcc cataagagcg atagtaagag atattctacc
     5881 tcctaagaaa attcttccta acagatctct tccataacga tctgtaccaa gaagatgtat
     5941 tgaaccttct tcaacagcaa tgaaatgcag gttagagtgt agtcctaaaa tcttataatt
     6001 ccatcccctg gcaaaaaacc ttatgtagag aattttactt gtatcctcca cataaattct
     6061 gctataagtt actggatcta catctaattt cagatcatat acgaagggcc tccagtggaa
     6121 ctttccatca gcatcaacga agcggactct ctgcggggga caatatgcct tctcgggatc
     6181 atattccata taatcgtgag gagagaaaaa gggagcgaaa acggacaaga ttgtcaggat
     6241 tacaataatt atccccccta ataatcccat tttgctcttt ttgaatcttc tccaaacaag
     6301 ctgaagataa ctttttcctt gttccgcttc tataatattg actgttttgg tttctttctt
     6361 ctttctaatc atatcgtatc ctcggatcta tccatgcaag tacaatatct gctagcagat
     6421 ttccaattac taggactact gctaataaaa gcaaaaagga cccagcaagg tacatatctt
     6481 gagtattaat agcgttgagg aacattggtc cagctgtggg tatatttaga acggtactgg
     6541 ctataagttc cccttgaatc ataaatggga gaaccatgcc ctgatacata ataagcggat
     6601 gtagcgcatt tggcaccgca tgtttgaata taacttctct ttcttttagt ccttttgatc
     6661 gtgcagtaag aacataagga agattgagaa cgtcaagaag gtttcctcgc atcactctca
     6721 tatttctggc cgttccggca aaacccacga ttactatcgg aatccagata tggctaagca
     6781 aatccagaaa tttcccccag ccccatggag caagtatgta gcgctgggaa aacaatcccc
     6841 ctgcctctac acccacttta acagccagta tatacattgc aatgagtgcc aggaaaaaac
     6901 ccggtacaga aagacccaca aaagcaaaaa aagtggctac gttgtcacca atactgtatt
     6961 gatgggtcgc cgaatagata cctatcagta tgcctaccag ggtagacaca agatgacaca
     7021 gagaagctat aagaagggtc catcctattc tctcccatat tacgtctgca actggttttc
     7081 tgtactggaa cgagaaacca aaatctccct tggttactat gtttttgatc caaacaaagt
     7141 actggatgta aagtggttta tccaaaccat agctatgtct tagctgctca gctactttca
     7201 ccgcttcatc ttccgttaac ccacccaggg attgcaactg agcctgcacc gtagacatat
     7261 aatccccggg aggaagctga attactatga aaacaacaat actgattatt ataagtacgg
     7321 gtatactcat taaaagtctc tggataacgt accggctcac aaaaaactac ctcccccggt
     7381 ggaggaaggg tgtcaccctt cctccacacc tcacaatagc cagttcattt ttgcttttct
     7441 tcatacttta atactttaca actactcctg gtaaaaattc tggaagttgc tcctcttctg
     7501 gcgtccatac ctgttcttgc ataacgttga gatggaacca ttgatggaat cttgccggca
     7561 cccctatagg aatgtttctg aacctttttg taattccagt attataaata ccttctatca
     7621 cacctatggt gtacaggttt tcggtccaca agttctgaag ttcactaaag agttcctgtc
     7681 taccttctcg agtcctctcg tatttcaacg cttctgctat ctctgccatt ctttcttcaa
     7741 agggtaaaag gtctcttttt tcgccaggac ctgctctgtg ccagttagga gacgaaggag
     7801 taacaggggc aagtgatgta agagaggtat aaggagaagc cagaaagtct tgcctggtca
     7861 tattgagctc aaattcacca gctgtatcca gtgcctgtcc cacagttgcc tttacaatct
     7921 taggcgtcag tcggattcca acttcttcaa acatgggaac gagcacttcc actatgtcaa
     7981 cgcaggcagt ctgatcttca tgtattttaa ctgctacagt aagattctgc ccctgtaacg
     8041 gaccagacgt ccaattgaga attccatctc catctgtatc cttaaattcc agccctgcaa
     8101 gtaactcttt agacttttcg gggtcataag gatacgacac cactgcttcc ggatcatagt
     8161 aatctgcccc ggaaggaaaa ccacttggca aagctctcag catcggaccc gggacaagca
     8221 ttctcgctac cgcttctcta tcaatagcat gtgaaattgc tttcctgaag tcaaggtttc
     8281 ggagaagttc tctcattgcc agatctgttt cattttttac tccctggtat agggataagt
     8341 ttgcctctaa ggcaaacgtc aatgtccaca tacctggaac aatcttaaat ggtgagccag
     8401 gcaaccttgc ttgttcgtta atgaaggtaa agagcccagg ggtttcgaga tgtgtctgat
     8461 cacatgtacc agcaatcagg tctaagtccc tttgaacacc cgcacgagct tttttaaagt
     8521 aaaattcacc aatgtatgga agttgttttc cagtttcatc taccttccag taatatgggt
     8581 ttcgcttcat aataagagct tcatcttctt tgagatatac cgggacccat gcaccgagta
     8641 ccactggagg aagatcatca ggaggtaaag cattgatgaa ttcttcataa gtcttctccg
     8701 agttatactt aggatggtgg ggcttcagaa catgagcagg tgctactgag aagtcaagca
     8761 aatccatata ccaaagtaaa tacactggaa aagcttcagc aaagtgccat tttatggtat
     8821 agtcatcgac tttttcaagt tctacaactt tgccatctaa tgtccacgta cctgcaccct
     8881 gccagctggg aacattggga tccagaatac agtcgtaata ggtgaaaaga acatcatcag
     8941 cggtgaatgg atgcccatca gaccattttg cgccttctac aagatacatg gttaaagtct
     9001 taccatcttc agaccactcc caatgagttg ccaaatttgg accaggctca ccatctttta
     9061 gtctccaaga cattccagta ttcacaagag cctcttgcat ggcttcttct atcccgaacc
     9121 aaccagctat cactcctgca gcccagttcc atccagctgt tggcactgca gaaacagttc
     9181 tccaggcatc tccataaata cctggtccat ctgacatccc actggtcata acaacagcag
     9241 gcttagcagg aagtcgctct tccaccggtg gaagctctcc ctttttaacc gcttcggcaa
     9301 gctctggagc ctcactatag cttggaagtg ctttatattg aatgatttca ccaacctcga
     9361 attttggtat ttcgatttct tgagccagag aagaagtaac aataaccaac acaaccacta
     9421 taatacttaa agttaatacc gaccattttc ttttcacttt tactgtcctc ctcaaaaact
     9481 tttctcaaca caaataacat ccaataaata acactttcct acccttgtaa cataacttca
     9541 ccgttgtatt tatggaacta aacttaagtt tttctcttta ccacctcctt tatcctcttc
     9601 ccctggtcga cttcctcacc accaattttt cttccaaaac tacttgtcga gtaggagaag
     9661 actcttcatc tttgagtcgg ctaaataaaa gagaggcagc cgttctcccc gcttcataag
     9721 taggaagagc aacgctggta agaggaggct ctaagacttc ggcaattatg tcatccaccc
     9781 ccaccagaga catatcctca ggaactttta tgccatcttc tcgagccgcc tctaacaccc
     9841 caatagctaa ttgttcacta aaagcaacaa tagcagtggg cttttgaggg tagctaaata
     9901 aaattttagc ctggagataa ccgctttctt gagaaaagtc ttcggcatcc accagaaaat
     9961 gagggttaat ttctgctcca aaatcttcca aagctttctt gcacccttca aagcgacgtt
    10021 tgcttatacc actttctttt accctcaata tggcaatttg ccgatgtccc aattctaaaa
    10081 gatgatgaac agcctgataa caacctttct ctgcatctcc tataactata tcagaagcgc
    10141 aaagccgggt agagccataa cagttaatta ctggtatcct ttgctttttg aaagtctcac
    10201 acatatcatt caacacctgg ttataaggag tggaatagat aattccttcc acccgatggc
    10261 gaagaaacat cttgacaaat cgttcagttt ttatgatatc gttatcatta ctacagataa
    10321 tcacgttgta tccttcctca aaagccacat cctgaatccc tcgcacaatt cgagaataaa
    10381 aggggttagc tatatcaggc aggagatatc caatggtcat agttttatta gtacgtaagc
    10441 ttttagctaa cgcattatag ttataattga gctttagagc agcattaaaa accttctcct
    10501 tagtttcttc tgtaacatat ccatatccgc ctaaagctcg actggcagtg gctacagaaa
    10561 ctccagcctc tctggctaca tcttttatag tgattctttt atctttcata ttttctcctc
    10621 aaatttaaga aaacgttatc atattatcct gctcctgtca actatatcct taaaactttt
    10681 gtgattactc tacgatgaca ggataaaaaa atgggcagac tattctccta agcaattttt
    10741 gcataatgtg gcaatcttcc tttttctcgt tctccctctt tcgagggggc aagagagaat
    10801 ataaggtaag ggcttcccat ccagagagag aagaagaaac gttcaagaac cacagctcct
    10861 ttttgtcatt tccaggacta ataattgaga ataccctcct taccatcatt cgtaaaacct
    10921 ctaatcagga attcggggat gacgaagaag gggttatgag cttagcatta tccatattta
    10981 tctggtctgg acacaaaaag aggtttttgc tgggtgtggc aatccctttc agggtcaatc
    11041 gcgagcactg ggagagacgt tttttctctt tcagtagttt ttgtttttta tgttttttgt
    11101 ttttgggggt tataaggggg tggcgcttct ttttcgtcat cccgagatac aggcgggaat
    11161 ccaaatgctt ttattctaaa taatatagat tcctgataga aacattcagg aatgacggca
    11221 agaagacacc ctcaccctaa ccctctccct tcaagggcga gggaaagaag atgtagagct
    11281 ctcccgagga gggcttctct tttcgtcatt cccgagatct ttaatcggga atccacgttt
    11341 ttaaatactt atagaccccg gcttgaaaat tgccggggtg acagacgagg ggatgataga
    11401 aacattcagg aatgacgttc ccttcctgtc attcccgaga tctttaatcg ggaatccaga
    11461 tgcatttgcc ctccaataat ttagaccccg gcttaaggat tgccggggtg acagacgagg
    11521 ggatgataga aacattcagg aatgacaaaa aaagaagtat aaatacactc aggaatgaca
    11581 aaaaaagagg aaagacaccc tcaccctaac cctctccctt cgaggg
//
LOCUS       DAOSVE010000053        11529 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_3176_length_11529_cov_69.036169, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000053 DAOSVE010000000
VERSION     DAOSVE010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 11529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11529
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3176_length_11529_cov_69.036169"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            677..1534
                     /locus_tag="PK016_05605"
     CDS             677..1534
                     /locus_tag="PK016_05605"
                     /inference="COORDINATES: protein motif:HMM:NF025107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXL02578.1"
                     /translation="MKEKFIDSKWHFTLIENSVIEDERLLPSEKLVYIVLSKFASRER
                     CCFPSLSTIAKLSGYSKSTVVRAINRLCHLGYLRKERRKDEIRGNISTFYTLTLPEDT
                     SLPNAPLPEEAPEESTPEEEEFLFDSTPCINDTSPCLMMTQALYHDDTTLVSPVDHND
                     IYYNDIHLTRDNKYITYVRRDAPEAKREIVTHLYHQLKDLGISKSPSWFGKQMKIAEN
                     LLSRYPPDVIFSVIDFAFQDCFWKTSFDGLEKMEKVLQKMKGEKNDRKLSDEEYYYGC
                     EPRGCRILR"
     gene            1482..2243
                     /locus_tag="PK016_05610"
     CDS             1482..2243
                     /locus_tag="PK016_05610"
                     /inference="COORDINATES: protein motif:HMM:NF005992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXL02579.1"
                     /translation="MKNITMDVNPGDAEYSANRCEVCGEERLLRYKEWEGEYLIYRCS
                     CEVEREKEKVLHKKEEERKERIKKLFSQSKLGKRFSQCSLENFEVVEGVEKAFRAIKK
                     FIDSFPPPGGEGLVLYGPPGGGKTHLSAACARELLEREYAVIFEQSAELMYRFNRTYI
                     SREESEDEIMQALISADLLILDDLEKGKWTDKVEERMYVIVNGRYRDMKPLILTTNLN
                     PPELENLVGRAIFDRLREMVTFVPLVTVSFRKKEV"
     gene            2245..2403
                     /locus_tag="PK016_05615"
     CDS             2245..2403
                     /locus_tag="PK016_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02580.1"
                     /translation="MTYNQVIFVGTMERAPRVFTGERPLVSLFLTFLSFLSAFIGNPL
                     RKTLFLPQ"
     gene            2581..2745
                     /locus_tag="PK016_05620"
     CDS             2581..2745
                     /locus_tag="PK016_05620"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HXL02581.1"
                     /translation="MRGVIFTICGHPEEIKRWVIGYGAHAEILEPEYLRKEVEKEIEK
                     LQEIYHSDRK"
     gene            3144..5555
                     /gene="cas10"
                     /locus_tag="PK016_05625"
     CDS             3144..5555
                     /gene="cas10"
                     /locus_tag="PK016_05625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Cas10/Csm1"
                     /protein_id="HXL02582.1"
                     /translation="MNEEQIVLGALLHDVGKALERCRYFDLPGDLQGTNVSYPHAKYS
                     AFLIRTLRSAQNSFLHPGLKGLLTEEVEKLVLFHHDPHTIEESILQVADWMSEAKREE
                     DEENTQVYYKVPLRSIFSRIGSEKTQPFYYPLRPLSFETLMPTTHNTIETANYKNLMD
                     KMLKDFPFIDTLEKLLGVLEIYFSSVPAQTSRFVSDISLYDHSRVTAALAHSLYLDWE
                     KGFFNEKDIEEIKKWILKKEGKSGNKDLFLIVGGDLSGIQNFIFNIPSKGAARSLKGR
                     SVYLDLIPRYVAKYILDQCHLTPANILYLGGGNFQLILPLSFEKELVEIRKKITSHLW
                     TLHRGEIAFILSSVKANLEDMFYFQLVLEKLNNSIAKDKEKRFWEIKETLYDQLFVPS
                     EEIVQEGEHCVSCGRKGTSSSSEEETLCPVCRSLVDLTSSLKNSKYLIEKRTNPTQQP
                     LKPSIFSFFTSLGYEISFESSFRKPRSNEKIYQLENLDIHAPDGTILDGFLSGSFTLP
                     DQTFDQIAETGIHSEDEGKKVGSPRLGYLKMDLDNLGSLLSELAGREKERSGEATSLS
                     RIRTFSRRVELFFNTYLVYLIREYDPGKTKIYPVYIGGDDLFMVGNWETIVHLAGKIR
                     DAFRAYTGGNPKFSLSGGIVCVPYDYPVIRTSSLVEEALTTSKTFLYYGEANPTKDKV
                     TIFNETLTWFELEIALELGEKIAQAINSGNSSRALIHRISNSLQDFAPLVSQSNQLVI
                     KPPAIWRFLYHLRKDREIADILEGIILNNLFKDQKIKNSRLILVADRLAEMKTRKVAS
                     VVDAY"
     gene            5582..6046
                     /gene="csm2"
                     /locus_tag="PK016_05630"
     CDS             5582..6046
                     /gene="csm2"
                     /locus_tag="PK016_05630"
                     /inference="COORDINATES: protein motif:HMM:NF015695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Csm2"
                     /protein_id="HXL02583.1"
                     /translation="MNMRAERNRTPGPYPRKDDTASQEERWRKNLRDVFSTPSYAEKI
                     LRFDRMEMDEFKEFNQKLKDFMRQQAGKINSSRMRKIYEMIKGAKNESDLLLTVPRLA
                     YIVGREERDRDAIGMVITLLSDAILAMKTGEDFKGIQKCAEAMVAYQKYYSD"
     gene            6065..6823
                     /gene="csm3"
                     /locus_tag="PK016_05635"
     CDS             6065..6823
                     /gene="csm3"
                     /locus_tag="PK016_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011973790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm3"
                     /protein_id="HXL02584.1"
                     /translation="MNQFLGKLFLNGKIETQTGLHIGGSRETGEIGGMDNPVIKTANS
                     IPYIPGSSLKGKMRCTLERAEGKEGARHSGEPCGCGHCLVCLLFGSHSSDDEKKTVSR
                     LCVRDAWLDIDDFKEKFPDLIEEKTFTEEKTENIIDRISGTAKHPRTIERVPAGSLFD
                     FEMVLNFFEDDRDQIDQLIRFFMRGLRMVEDEYLGGSGTRGSGKVAFRHLAFSLKRKL
                     EYVGNNTRLPLLTKENTTFDVDELINLLMSQLVG"
     gene            6829..7860
                     /gene="csm4"
                     /locus_tag="PK016_05640"
     CDS             6829..7860
                     /gene="csm4"
                     /locus_tag="PK016_05640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm4"
                     /protein_id="HXL02585.1"
                     /translation="MAVQVVKLLPLEHFRIRIGEGRMDRIGNLVHSDTLFSALVNSGI
                     KLYGEDLFAPMIDSLRLSSVFYGLRVKERNESRDLLFFPKPRARFREPEDPTQRKKLK
                     RLSWVSSSALSQICQNYDYEEALFQIDLLDPNQFVFLNPQFCVSSAERIPWLEENISF
                     FRTTLEPKVSISRVNAQSLGLYYQAELEFCFPKWTQERSITPFLFFLWEEELTPQLIG
                     TLNLFIEEGLGGERASGKGTFASWEYEPFPLPQEGKIMMLLSSAVPKREEMEQLISYE
                     LQKREGFLFWGGPVGKRKKSHYALKEGSLVKTPFEGTNLEVSPLPEKRAVAYGKALGW
                     AFGQKGGQE"
     gene            7857..8984
                     /gene="csm5"
                     /locus_tag="PK016_05645"
     CDS             7857..8984
                     /gene="csm5"
                     /locus_tag="PK016_05645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm5"
                     /protein_id="HXL02586.1"
                     /translation="MMKLSLSVVTPICVRSGENASAVADYIYDPQKRTIYLIDQVKFN
                     RWLMEQGKNTRFLIKDITALVSSRQGTLVHFFNEHGLSPRDFSRATIPVSFPNSITFG
                     NRNLFLPTLTGGKPYIPGSSIKGAIRTALMFDYIKNGDNGGLGHGATPHLQDSRQVYT
                     GSDIFCLPDNKSRYPSENDALRFLQVTDTTPSPLESLRVYALERKGGASSAIPALLLG
                     FHPGTEMTFEVNILPGFEKANIPDYWKKFFRKGEVAILKAIKNYSVTLLRDETDRLKK
                     RSDKPPENQLLYDSYTRILSNTKNNIKQGGAALLRIGFGKTYYFNSIGYFLSDEEKKR
                     YEKNWKRVKSVSEFPITRWMIRTPDNQCLPAGWSLIQEKRI"
     gene            8987..10702
                     /gene="csx2"
                     /locus_tag="PK016_05650"
     CDS             8987..10702
                     /gene="csx2"
                     /locus_tag="PK016_05650"
                     /inference="COORDINATES: protein motif:HMM:NF021006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02221 family CRISPR-associated protein"
                     /protein_id="HXL02587.1"
                     /translation="MLITSIGKAGKYRETNYTFHGKSCSSCFSTLAIAQLLDIPREEK
                     ILLLVTPETVEASSDIQSQFEKAEYHNLEIVPIPHAEDEESIFQIVLTLTNAVAEKEQ
                     IILDVTHSFRSLPFVYFVTLLFLYAFKEVDIKGIYYGSFEEGREANPIVDLTSLFSMA
                     FWFYAVQTFRETGSVQPLYDIARREASRIIRHKEKKEIGYKLSALKVPLQNLGLSISF
                     CLPLEMGMDVEQFLKKLNFQPDNGSYERLLAVVFQKISDELKEFALSSNKKDEVLLTQ
                     EELVREYKIARWYNHHGNPDHTLIILGELMINWMIKVRNMKGWLDYYNTRDKVRGTLN
                     GMSKRSREDLGPDNIIKKIGSCWNSIGNQRNEFAHAGLKNEIIRTTGKKEAVDNLCSA
                     VYDLLQVDVLNTIPPKERKKLLIVPLGLSPGVLYSAALLSRFDDLLVIFSEQTRPLLP
                     STISEIRKSRRVPDQNVFTLLLGDAHYDFIPAGQLKDFTVIPLKGEQVSVEHFLCNYD
                     SVEGCITGGTTAMQYIVERLLDVARGVGVFRINRFALIDRRPPEEQRAHPFILGDKIV
                     LDQER"
     gene            complement(11343..>11529)
                     /locus_tag="PK016_05655"
     CDS             complement(11343..>11529)
                     /locus_tag="PK016_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02588.1"
                     /translation="LTLSLQWRGKKDVKLSRRGRGKICRFRRRRGKKDVKLSRRELLF
                     PSCLSALIRHPDAFALK"
BASE COUNT         3318 a         2311 c         2561 g         3339 t
ORIGIN      
        1 tacatatttt ccctcgccct tgaagggaga gggttagggt gagggtgtct tcttgcctgt
       61 cattcctgaa tgcatttatc actcatagcc gtcattcctg aaggtttcta tcacctcgtt
      121 gctcatcatt cttgaacgtt tctatcagga atctaaatta cctaagggca agaaccttta
      181 gattcccgac tgaaacgctc gggaatgaca aaatagagag aatagccccc tctttttctg
      241 tcacccctga atgcctctat caggggtcta tggtctttaa aaacatagat tcccgactga
      301 aacgctcggg aatggcaaaa tagagggagt gatagaggaa aaccatcaga tggttactta
      361 ctttataacc cggagtaata tctcttatca gtcgcctgct tttattgagt tataaagata
      421 aatatttata agacaatatt aaatatggtt gatttttagt aatagttgtc ctataatcag
      481 gatagtcctt tttgaaggac agacatataa atataagaga cagaaaaagg gaggtgttac
      541 tgagataatt tacccccggg gatagtaatt actgccctgc ttttagtttt ttaatattaa
      601 tatccggggt agcgagagaa cttctccttc cctccaaata acgtttagta agaatactac
      661 cggtaaggag gatgcaatga aagaaaagtt tattgatagt aaatggcatt ttaccctaat
      721 agaaaattcg gtgattgaag atgaaaggtt gcttcccagt gaaaaattag tgtacattgt
      781 cctttctaaa tttgcctctc gggagaggtg ttgttttcct tctctctcta ccattgctaa
      841 actctctggg tattctaaaa gcacagtagt gagagctata aatcggcttt gtcatttggg
      901 ctacctcagg aaagaaagga gaaaggatga gataagagga aatatttcca ccttctacac
      961 tctcaccttg ccagaagata cttccttacc taatgctccc ctcccggaag aagctcctga
     1021 agaatctacc ccggaagagg aagaatttct ttttgactct accccttgta tcaatgacac
     1081 cagcccttgt cttatgatga cacaagcctt gtatcatgat gacactaccc ttgtatcacc
     1141 agtagaccat aacgatatat attataacga tatccattta acgagagata ataaatatat
     1201 tacttacgtc cggagggatg ctccggaagc aaaaagagaa atagtaactc atctctatca
     1261 ccaactcaaa gatttaggaa tctctaaatc tccttcctgg tttggtaaac aaatgaaaat
     1321 agccgagaac cttctttctc gttatcctcc tgatgttata ttttcagtaa tagactttgc
     1381 ttttcaggat tgtttctgga aaacttcttt tgatggttta gagaaaatgg aaaaggtatt
     1441 acagaaaatg aaaggagaaa aaaatgacag aaagctcagc gatgaagaat attactatgg
     1501 atgtgaaccc cggggatgcc gaatactccg ctaatagatg tgaagtgtgt ggagaggaac
     1561 gtcttttaag atataaagag tgggaaggag aatacttaat ctaccgctgt tcctgtgagg
     1621 tagagaggga aaaagagaag gtgttacaca aaaaagaaga ggaaagaaaa gagaggataa
     1681 agaaactctt cagtcaatct aagctgggta aacgtttctc tcagtgtagt ttagaaaatt
     1741 ttgaagtggt agaaggagta gaaaaagctt tccgagcgat aaagaaattt atcgactctt
     1801 ttcctccacc cggaggagaa ggattagttc tctatggacc cccggggggt ggcaaaactc
     1861 acctatctgc tgcctgtgcc cgagaactcc tcgagagaga atatgcggtt atctttgagc
     1921 aatcagctga attaatgtat cgttttaatc gtacttatat ctctcgggaa gaaagcgaag
     1981 atgaaattat gcaagcttta atatcggcag atttactgat tcttgatgac ttagaaaaag
     2041 ggaaatggac tgataaagta gaagagcgga tgtatgtgat agtgaatgga agataccggg
     2101 atatgaagcc tctcatcctc accaccaact taaatccccc cgaattagag aatttagtgg
     2161 gaagagctat ctttgaccgg ttacgggaga tggtaacctt tgtcccttta gtaactgtga
     2221 gctttagaaa aaaggaggtc taaaatgaca tataatcagg ttatttttgt aggaactatg
     2281 gagcgagcac cccgggtatt cactggcgaa agacctctgg tgagtttatt cttaactttt
     2341 ctgtcattcc tgagcgcttt tatcgggaat ccactgagga agacgctttt tcttcctcag
     2401 tagtttttat tctttatgtt ttttgttttt ccaaaggggt gatagggggt ctttttcccc
     2461 ctatagtaag gaaggttctt tcttccttac tgttagtttt tttctttttg tgttttattt
     2521 ttgagttttt gtctttccgg gggtcatagg gggatcggtt ttccccctat agtcggggtt
     2581 atgaggggag ttattttcac catttgtggt catcccgaag aaattaaacg ttgggtgata
     2641 ggttacggcg ctcatgccga gatcttggaa cctgaatact tgcgaaaaga agtcgaaaaa
     2701 gaaattgaaa aattacagga aatttatcac agcgacagga agtgacaccc ccattgggta
     2761 tgatgagaga gaaagtaaaa aaatatcctt catcattgaa aataaatttt ggggtggaat
     2821 tatggataac ggaatgaaaa ctaataaaga cagagtttgt cctcagtgca aaggcactgg
     2881 ccgttgtaaa tattgtagtg gttcgggaaa acatgcttac cccggatacg gaaaaccaag
     2941 cgaccaacct tgtatgtggt gttttggcac cggagtttgc cagcagtgta agggaaaagg
     3001 ttattattaa agctacctga ggtgttcgag ttttttggag agtagtaaca aattattggc
     3061 ttaaactttt acatataatg tcgtataatc tttataattt gaagctgtag ttaattttgt
     3121 tcctttcttg aggggggtaa taaatgaacg aagagcaaat tgtgcttgga gcattacttc
     3181 atgatgtagg aaaagctctg gagcggtgtc ggtattttga tttgccaggt gaccttcagg
     3241 ggactaatgt ttcttatccg catgctaaat attctgcctt tttaatccgg actttaagga
     3301 gtgctcaaaa ctcttttctc catcccgggt taaaaggctt acttactgaa gaagtggaaa
     3361 agttagttct tttccatcac gatcctcaca ctattgaaga atcaatcctt caggtggcag
     3421 attggatgtc agaggcaaag cgggaggaag atgaagaaaa tacccaagta tactataaag
     3481 ttccccttcg ctctatcttt tctcgaatcg gtagcgaaaa aacacaacca ttttattacc
     3541 cgcttagacc cctcagcttt gaaaccctta tgcccaccac tcataatacc atcgaaacag
     3601 caaattataa aaatctcatg gataaaatgc taaaagattt cccttttatt gatactcttg
     3661 aaaaactatt aggagtgctg gaaatctatt tttcgtcagt tccagctcaa accagtcgct
     3721 ttgtgagcga tatatcactc tatgaccatt cccgggtaac agcagctctt gcccattctc
     3781 tttaccttga ttgggaaaag ggctttttta atgagaaaga tatagaggaa atcaaaaagt
     3841 ggattctaaa aaaagagggg aaaagcggaa ataaagacct ttttctaatt gtaggtggag
     3901 acctctccgg aattcagaat tttatcttta acattccctc taaaggtgca gcgcggagcc
     3961 tcaagggacg ctcagtttat ttagacctca tccctcgata tgtggccaaa tatatcttag
     4021 accaatgcca tcttactccg gcaaatattc tctatttagg gggaggaaat tttcagctca
     4081 tcctgcctct ttcctttgag aaagagctgg ttgaaattcg gaagaagatt acctctcatc
     4141 tctggactct ccaccgggga gagattgcct ttattctttc ttcagtcaaa gcaaatctcg
     4201 aagatatgtt ttatttccaa ctggttcttg aaaaactcaa taacagcata gcaaaagata
     4261 aagaaaagcg tttttgggaa ataaaagaaa ccttgtatga ccagctcttt gttccgagtg
     4321 aggaaatagt tcaagaaggg gaacactgtg tttcgtgcgg tcgcaaggga acgtcttcct
     4381 ctagcgaaga agaaactctc tgtccggttt gccggtcact ggtggatctc accagctcat
     4441 taaaaaattc taaatacttg atagagaaaa gaactaatcc aactcaacaa ccattaaagc
     4501 cttctatttt tagttttttt acttccctgg ggtatgaaat ttcctttgag agttcttttc
     4561 gtaaacctcg ctctaacgag aaaatttacc aattagaaaa cttagatatc catgctcctg
     4621 atggaactat tttggatggg tttctctcgg gaagcttcac tttacccgac caaacctttg
     4681 accagattgc agagaccggt attcattccg aagatgaagg taaaaaagta gggagccccc
     4741 ggcttggtta tctcaaaatg gacctggata acttgggaag tttgctctct gagcttgctg
     4801 gaagagaaaa agaacgttct ggagaagcta cttccctctc tcggattcgc actttttccc
     4861 ggcgggttga gctctttttc aacacctatc ttgtctatct cattcgggaa tacgacccgg
     4921 gtaaaaccaa aatctatcct gtgtatatcg gtggagatga tttgttcatg gtgggcaatt
     4981 gggagaccat cgttcacctt gctggtaaaa ttcgtgatgc atttcgtgcc tataccgggg
     5041 gaaaccctaa gttctcgctt tcggggggaa tagtttgtgt tccttatgat tatccggtta
     5101 tccgaaccag ctccttagta gaagaggcac tcaccacttc gaagaccttc ctttattatg
     5161 gagaagcaaa ccccactaaa gataaggtga cgatttttaa tgaaacttta acctggtttg
     5221 agttggaaat tgctctggag ttaggggaga aaattgctca ggctatcaat agcggtaact
     5281 ccagtcgggc tctgatacat agaatttcta attccctgca ggattttgcc ccccttgtta
     5341 gccaatctaa ccagctggtc atcaaacccc cggctatctg gagatttctc tatcatctca
     5401 ggaaagatag agaaattgcc gatattttag aaggaataat tttaaataat ctctttaaag
     5461 accaaaaaat caaaaattcc cggcttattc tggttgccga ccgcttagcc gaaatgaaaa
     5521 ccagaaaagt tgcttctgta gtagatgcat attaattaat aaaaggagag tggacaaaaa
     5581 gatgaatatg agagcagaaa gaaatcggac gccagggccg tatccccgta aagacgatac
     5641 tgcttcccaa gaagaacgtt ggcgtaaaaa tttgagagat gttttctcaa ctcccagcta
     5701 cgctgagaaa attttgcggt ttgaccggat ggagatggat gaatttaaag aatttaacca
     5761 gaaactaaaa gattttatga gacaacaggc aggtaagatt aactcttccc ggatgcgaaa
     5821 aatatacgag atgataaaag gagccaaaaa cgagagcgac cttctcctca ctgttcctcg
     5881 gctggcttat attgtcggtc gggaagagcg agaccgtgat gcaatcggaa tggtaattac
     5941 gctccttagt gacgctatcc tggcaatgaa aaccggggaa gattttaaag gcattcagaa
     6001 gtgcgctgaa gcgatggttg cctatcagaa atattactct gattaataat gaggagggaa
     6061 tgaaatgaat caatttctgg gaaagctttt tcttaacggg aaaattgaaa ctcaaaccgg
     6121 ccttcatatc gggggctctc gagaaactgg agaaattggt ggtatggaca atccagtgat
     6181 aaaaactgcc aatagcattc cctatattcc tggttcctcc ttaaaaggga aaatgcgctg
     6241 cactttagag cgagcagaag gtaaggaagg agctcgccat agcggtgagc cctgtggatg
     6301 tgggcattgt ttagtttgtc tcctttttgg aagtcattct tcagatgatg aaaagaaaac
     6361 tgtgagccgc ttatgtgttc gggatgcctg gctggatatt gatgatttca aagagaagtt
     6421 tcctgacctt attgaagaaa aaaccttcac tgaggagaaa acagaaaaca tcatagaccg
     6481 gattagtggt acggcaaaac atcctcgtac tattgaacga gtcccggccg gctctctctt
     6541 tgactttgaa atggttctta acttttttga agatgaccgc gaccagatag accaactcat
     6601 tcgtttcttt atgagagggc ttcgcatggt ggaagatgaa tacttaggag gaagcgggac
     6661 ccgggggtca ggtaaagtgg cctttcgtca tctggctttc agcctgaaaa gaaagctgga
     6721 atatgtaggc aataatactc gtttaccgct tcttaccaaa gagaatacca cttttgatgt
     6781 tgatgaatta attaacctgc ttatgtctca actggtgggg tgagagttat ggctgttcag
     6841 gtggtgaaac tactccccct agagcatttc cggatacgaa taggagaggg gcgaatggat
     6901 cggattggca acctggttca ctctgatacc cttttctcag cactggtaaa tagcggtatc
     6961 aagctctatg gcgaagacct ttttgctcct atgatcgatt ctctgcgtct ttcctcagtt
     7021 ttctatgggc tgcgagtaaa agagagaaac gaaagtagag accttttgtt cttccccaaa
     7081 cccagagccc gttttcgtga acccgaagac cccactcaga ggaaaaagct caagcgacta
     7141 tcctgggtat cttcttccgc tttatcccaa atttgtcaaa actatgatta tgaggaagcc
     7201 ctttttcaga ttgacctcct tgaccctaac caatttgtgt ttcttaatcc tcagttttgt
     7261 gtatcttccg ctgagagaat cccctggctt gaggaaaata ttagtttttt ccggacgacg
     7321 ttagaaccca aagtttctat cagcagagtc aatgctcaat ctctgggtct ttactatcag
     7381 gcagaactgg aattttgttt tccgaaatgg actcaagagc ggtctattac tccttttctc
     7441 ttttttcttt gggaagaaga gcttactcct caactcatcg gtactctcaa tttgttcatt
     7501 gaagagggac taggagggga aagagccagt ggaaaaggaa cctttgcctc ctgggaatat
     7561 gagcccttcc cgcttcctca ggaaggaaag ataatgatgc ttttaagttc tgccgttcct
     7621 aaaagagagg aaatggaaca gctcatcagt tacgagcttc aaaagcgaga ggggtttctc
     7681 ttctggggtg ggccggtagg gaagcgaaaa aaatctcact atgctctaaa agaggggtcg
     7741 ctggtaaaaa ccccttttga gggaacaaac ctggaagttt ccccgcttcc tgaaaagcga
     7801 gctgtggcat atggaaaagc attggggtgg gcgtttgggc agaaaggagg tcaggaatga
     7861 tgaagctatc tttgagtgtc gttaccccta tctgtgttcg aagtggagaa aacgcatcag
     7921 ctgtggctga ttatatctac gacccccaaa aaagaacaat ctatctcatc gaccaggtaa
     7981 aatttaatcg gtggttaatg gagcaaggga aaaacactcg gtttttaatc aaagatatta
     8041 ccgccttagt ttcttcccgc cagggaacac tggtacactt ctttaatgaa cacggtctct
     8101 cacctcgcga tttctctcgg gctactattc cggtttcttt tcccaattca ataacttttg
     8161 ggaatcgtaa ccttttcctt cccactttaa ccggagggaa accctatatt cccggttctt
     8221 ccattaaagg agccatccgg actgctttga tgtttgacta tatcaaaaac ggggataatg
     8281 gtgggttagg acacggtgct actcctcacc ttcaagattc ccgccaggtg tataccgggt
     8341 cagatatttt ttgcctgcct gacaataagt ctcgctaccc ttcagaaaat gacgctctcc
     8401 gattccttca ggtaaccgat actacccctt ctcctttaga atcactccgg gtctatgctt
     8461 tggagcgtaa aggaggggct tcttctgcta tccctgctct tcttttggga ttccatcccg
     8521 ggactgaaat gacttttgag gttaacatcc tgccgggttt tgagaaagca aatattcctg
     8581 attactggaa gaaattcttt agaaagggag aagtggccat tctcaaagcc atcaagaatt
     8641 attcagtcac cctccttagg gatgaaactg atagactgaa aaaaagaagt gataaacctc
     8701 ctgagaatca attgctttat gatagctaca ctcggatttt aagtaatact aaaaataata
     8761 ttaagcaagg gggagcagct ctcctgcgca ttggtttcgg aaaaacttac tattttaatt
     8821 cgatcggata ctttttgtca gacgaggaaa agaagagata cgagaaaaat tggaagaggg
     8881 ttaagtcagt tagtgaattt ccaattaccc gctggatgat tagaaccccg gataatcagt
     8941 gtctgccggc ggggtggagt ttgattcaag aaaagagaat ttgaagatgc ttatcacctc
     9001 gattggtaaa gctggaaaat atcgggagac taactatacc ttccatggga agagctgctc
     9061 gagctgtttt tctactctgg ccatcgctca gttattggat attcctcggg aagagaaaat
     9121 tttgctttta gttactcctg aaactgttga agcttcttct gatattcagt ctcagtttga
     9181 aaaagctgaa tatcataacc tggaaatagt gcccattccc cacgctgaag atgaggaatc
     9241 tatttttcag atagttctca ctctcaccaa tgcggtggca gagaaagaac agattattct
     9301 cgatgttact cattcttttc gtagccttcc ttttgtttac tttgtcactc ttctttttct
     9361 atacgctttt aaagaggttg atattaaagg aatttactat gggtctttcg aagaaggaag
     9421 agaagctaat cctattgtag acctcacttc cctcttttct atggctttct ggttctatgc
     9481 cgtgcagacc ttccgggaaa ccggtagcgt gcagcctctt tatgatatcg caaggaggga
     9541 agcatcccgg attattcggc acaaagaaaa aaaagaaatt ggctataaac tctcggcctt
     9601 aaaagttcct ctccagaatt taggcctatc tatatctttc tgtctcccct tagaaatggg
     9661 gatggatgta gagcagttcc taaagaaatt gaattttcaa cctgataatg gttcatatga
     9721 gaggttattg gcagtggttt ttcaaaaaat atccgatgag ttaaaagaat ttgctctatc
     9781 ctcgaataaa aaagatgaag tccttctcac ccaggaagaa ttagtgaggg agtataaaat
     9841 agcccggtgg tataaccatc atggaaatcc agaccatact ttaatcatct taggagagct
     9901 tatgataaac tggatgatta aagtccggaa tatgaagggc tggttggatt attataatac
     9961 ccgtgataag gtcagaggaa cccttaacgg gatgtcaaaa cgttccagag aagaccttgg
    10021 cccggataat atcatcaaaa aaattggtag ctgctggaac tccattggaa accagagaaa
    10081 tgagtttgct catgctggtc ttaaaaatga gatcattcgc accacaggaa agaaagaagc
    10141 ggtggacaac ctttgttctg ctgtttatga cctgttgcaa gtggatgttt taaataccat
    10201 tcccccgaaa gagcggaaga aattgctcat cgtccccctt ggcttatcgc caggggttct
    10261 gtattcagca gcacttttga gccgtttcga tgaccttctg gttatcttct ccgagcagac
    10321 tcgtccgctt ttgccctcta ctattagtga gattcggaaa agtagaagag taccagacca
    10381 aaacgtgttt accttattgc tcggtgatgc tcattatgat tttattccgg cagggcagtt
    10441 gaaagatttt acggtgattc cccttaaagg agaacaggtg tcggtggagc attttctctg
    10501 caattatgat agcgttgagg gatgtatcac cgggggtact acagccatgc agtacatagt
    10561 agagcgattg ctcgacgttg cccggggtgt tggtgtattt cgcattaacc gttttgctct
    10621 cattgatagg cgtccgccgg aagagcagag agctcatcct tttattctgg gagataagat
    10681 agttcttgac caggagcggt gacaaataga gaaccacaga gtctatagta atgtttcact
    10741 tctcattcct gaatgcctct atcatccagt tgctcgtcat tcctgaacgt ttctatcagg
    10801 aatctaaatt acttaagaat aaaagcattt agattcccga ttaaagattt cgggaatgac
    10861 ggaagagggt cattcctgaa tgtttttatc aggaatctaa attacttaag ggcaaatgca
    10921 tctggatgcc tgattaaagc gctcaggcat gacggaaaga gaagctctcg acgggagagc
    10981 tttacatctt ttttccctcg ccctcgacgg aacctacata ttttccctct ccctcgacgg
    11041 gagagggtta gggtgagggt gtctttcctc aaaaaatact tttcctcagc aacttccccc
    11101 tccctcgctc accctgctaa atcactgatt tggcagggaa cccgggcact ccctccctcc
    11161 aggggcggga gaaaaaaatg cgggaaactc agtttttccc tctccctcga cggaacctac
    11221 atattttccc tcgcccttga agggagaggg ttagggtgag ggtgtcttct tgcctgtcat
    11281 tcctgagtgt ccctgtcatc cccttgcctg tcattcctga atgcttctat caggaatcta
    11341 aattacttaa gggcaaatgc atctggatgc ctgattaaag cgctcaggca tgacggaaag
    11401 agaagttctc gacgggagag ctttacatct tttttccctc gccttcgacg gaacctacat
    11461 attttccctc tccctcgacg ggagagcttt acatcttttt tccctcgcca ttgaagggag
    11521 agggttagg
//
LOCUS       DAOSVE010000054        11285 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_3251_length_11285_cov_65.410151, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000054 DAOSVE010000000
VERSION     DAOSVE010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 11285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11285
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3251_length_11285_cov_65.410151"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..817)
                     /locus_tag="PK016_05660"
     CDS             complement(176..817)
                     /locus_tag="PK016_05660"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HXL02589.1"
                     /translation="MKKIYDLTLNLTSDIPVFPGQPPLSFLPWSNLAVHGNATNAFFM
                     VEHTGTHLDAPAHFIEGGKTVEKISLECFWGEALVLDVSSYLSKGKLELSDLKRVLGG
                     QDIEEGDIVIFSSGVDKLLGRPEYFTQGVYLTESVAEFLRDKKVKGVGTDSPSIDSQP
                     FVVHRLLLAQEIIIYESLTNLEELRGKRVTFAAFPLKLKGCTGSPVRAIALCS"
     gene            complement(899..1609)
                     /locus_tag="PK016_05665"
     CDS             complement(899..1609)
                     /locus_tag="PK016_05665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXL02590.1"
                     /translation="MLESQKITILIPAYNEAPRLPKVLEVVSKIEWVDEIVVIDSHSQ
                     DGTQDVARQFPGDVIYLDSDRGKGAALVEGIRRKKDSDIYVFLDADLVALTEEHILAL
                     VNPLIEDQSLVMTIGVFEEGRRWGDLAQKLFPILNGMRGVRGEWVRNLPDFSWTRFGV
                     EVFLTRIARENKVNIELVPLTGLAHYHKEEKYGFFPGLYHRIKMYKEIVQTFFLYKKR
                     IEGYSKVTGEEEKDGVMA"
     gene            1713..2774
                     /locus_tag="PK016_05670"
     CDS             1713..2774
                     /locus_tag="PK016_05670"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HXL02591.1"
                     /translation="MNNSEEGKIIRNFYYLVLIIITLYLTYILRDVLIPFVLGGLLAY
                     ALSPAVQFLNSRGLSWKLSVIIIFIVVLAFFLSLFVFLVPEVLSQFRTLTSQWSQYSE
                     SLIDKILEGIEKLNERHPDSNISQTIESFLTTISTNLQNYLTHIISGIPNLVSILANI
                     IFLGFIMTPVALYFFMADATKVRRSLVRMFPFSRRREYIALFREIDQVLGGFIRGRAL
                     IALFVGICATVGLALMNIDFALAIGVIAGIVEIVPYLGPVVGAIPAIILAATKSPWLV
                     LGVALLFGGINFVEGIFISPKLLGKETGLHPLTVLFALAVGGKLYGALGIIIAIPVAG
                     VLKGLLFHYRKNKKLSSTS"
     gene            complement(2778..3506)
                     /locus_tag="PK016_05675"
     CDS             complement(2778..3506)
                     /locus_tag="PK016_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HXL02592.1"
                     /translation="MDESLIKKAASFMLERQPWLVLTGAGISTESGIPDFRSPGSGLW
                     EKYDPMEILSTDVLFNRPQIFYQEGLPVLMKFENAQPNKAHYILAEWEKKGLVEAVVT
                     QNIDSLHYKAGSQKVLEIHGHLRSGHCLTCKTSFPMEAIKEKVDEGDIPPHCHCGGII
                     RPDVVLFGDMLPPCFEEAVELAHRLPLLVIGSSLQVSPANFLPSYSPYLIIVNLEPTP
                     FDHRARFILRGKAGEILDDLDKAM"
     gene            complement(3517..4095)
                     /locus_tag="PK016_05680"
     CDS             complement(3517..4095)
                     /locus_tag="PK016_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02593.1"
                     /translation="MKKESLAQTFSLVLQPSAIATLLYLVVSFSFATGWRTWVFFLIG
                     LFFVGVFPILLVLFLTKKGKVSDPDLPNREERFFPYLMICSSYLLALVIFLLASAPLP
                     LVAITCCYLGVTFVGALISLFWKISLHLAGIAGPVTALVMILNPYWAFLYLLLVPLGW
                     ARIYLGKHNLYQVVAGSMMSMVITFIIVSSIV"
     gene            complement(4105..4674)
                     /gene="pth"
                     /locus_tag="PK016_05685"
     CDS             complement(4105..4674)
                     /gene="pth"
                     /locus_tag="PK016_05685"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HXL02594.1"
                     /translation="MYLIIGLGNPGEKYSLTRHNVGFRVVDRFSHQYDWKWINIGHRL
                     SWAEGEVKGKKVYLLKPLTYMNQSGEGLLYFPRFQLFFEGELIVVHDEMDFPPGVVRI
                     KKGGGTAGHRGLNSLVETLKRDDFLRIRVGIGKPPFREKAMDYVLGIPEGEEKILLEQ
                     GEEKAVSALSSILEKGWERAMNKYNVREN"
     gene            complement(4678..5343)
                     /locus_tag="PK016_05690"
     CDS             complement(4678..5343)
                     /locus_tag="PK016_05690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HXL02595.1"
                     /translation="MPKGNNVIINLERREEKGKEKSRKMRKEGWIPAVLYSRDSKDGK
                     AKLVKVREKELKRILQIPGITHHMLDLSLEGEMKKGIIRDLQKNPVKEEIWHVDFYEV
                     QTGQKITLSLPVRIKGEAPGVKAGGTLELVTPELEVECPSGAIPEDIEVDVSELEVGD
                     TIRVGDLKVPQDVTVMKDPDEVIVVVIPPKVEVVEEKIGEEAKGEVEEPQVIGKGKTE
                     EEK"
     gene            complement(5363..6331)
                     /locus_tag="PK016_05695"
     CDS             complement(5363..6331)
                     /locus_tag="PK016_05695"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HXL02596.1"
                     /translation="MFNGQEFSRHLKVFSGTANPLLAQEVCWYLDIPLGKADVGRFPN
                     GEIRVQIEESVRGCDIFVIQPTSPPANDHLMELLIMIDALHRASAERVTAVIPFYGYC
                     KQDRKTKGREPISAKLVANLLVTAGVDRILTMDLHAGQIQGFFDIPVDNLAAQSLLAR
                     YFLSRGRKEVVVVSPDVGGAARARAFANQLGADLAIIDKHRPTYSQVMVMNVVGEVRD
                     KRAIIVDDLIDTAGTLVEGAEALHKRGAKEIYACATHPVFSEPAFTRIEKSCLQEVVV
                     TNTIKVDQEKLSQQGVNRIKVLSVAPIFAEAIKRIHGELSVSELFI"
     gene            complement(6332..7720)
                     /gene="glmU"
                     /locus_tag="PK016_05700"
     CDS             complement(6332..7720)
                     /gene="glmU"
                     /locus_tag="PK016_05700"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HXL02597.1"
                     /translation="MNLSRWGLIVLAAGQGKRMRSSLPKVLLPAWGKPVMQYLLDELK
                     WGEFGQRFLVVSPSMEELMKSNFGQEMTIVVQKEPQGTAHAVSTVFPLIQEGIDWFLV
                     LYADMPLLRGETIFSFLSQAEEKHSPLSFLTSIAPHPAGFGRVVRNEKGEPLGIVEEK
                     DCSAREKKISEVSLGIFAFQREAFREIISLIGNRNAQKEYYLTDAVQVTREKGYRPEA
                     FVVEWDEQFLNVNTPADFSKAILILKERKIASLWDEGVKIIDPSATYIDWDVVVEEDV
                     WLYPGTVIEGKSIIEKGARIGPFSRISESRVGKNSSVEYSVVEGSFIGEEVRVGPFSH
                     LRPGTKLEQGVKVGNFVEVKNSQVGEGTKALHLSYLGDAQVGKKVNVGAGTITCNYDG
                     EKKNPTFIEDEVFIGSNNSLVAPVSIGKGAYTAAGSTITQDVPPYSLGIGRAKQKNIE
                     DWVKRKIRRNKK"
     gene            8028..8429
                     /locus_tag="PK016_05705"
     CDS             8028..8429
                     /locus_tag="PK016_05705"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HXL02598.1"
                     /translation="MVKLAKSMKEVINQLAQQIISSTAYKNFQEKRALLANHPEFLSL
                     FQQLEEKQKELDKLTDNEEDIPDEYLEELEDISKQLLSEPVFVDYLKAQDQLVSLLEL
                     INDELSSTLGFDFADSVNFLREEEEGEESWE"
     gene            complement(9187..10083)
                     /locus_tag="PK016_05710"
     CDS             complement(9187..10083)
                     /locus_tag="PK016_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HXL02599.1"
                     /translation="MTSSHPIAIERWAKSLVESYRKTEKITNIEKKHFPSRAKVIEIS
                     EELRELLFPGYLGRTNLCWLNTEYFVGGKLDYLFGELSQEIIFSLGDRSPEGEEKAHH
                     EVEIFFGKLPAVREMLEDDVQAAFDGDPAAKSLDEIIFSYPCLVAISIYRLAHELFVQ
                     GIPLIPRMMTEYAHSVTGIDIHPGAKIGRSFFIDHGTGVVIGETCEIGNNVKIYQGVT
                     LGALSFPKDERGKIIRGTKRHPTIEDDVTIYAGATILGGQTVIGKGSVVGGNVWLTHS
                     IPPYTKVAIEEPRLKISENQKL"
     gene            complement(10098..10325)
                     /locus_tag="PK016_05715"
     CDS             complement(10098..10325)
                     /locus_tag="PK016_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HXL02600.1"
                     /translation="MDCKEVLEKLYLYLDHEILTERERQQIEEHLKWCFKCCQMYELE
                     KNLENLIRINGRNDEVPPSLFFRIENVIARF"
     gene            complement(10304..10897)
                     /locus_tag="PK016_05720"
     CDS             complement(10304..10897)
                     /locus_tag="PK016_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXL02601.1"
                     /translation="MVKTKEEQKEYFQELLNPELGALFRTALRMTRNREDAEDLVQET
                     ITKAFAAFDRFEEGTNFRAWIFRILTNTFINNYYRVRDRQKLPSLDEMEEESFFQPIA
                     EGISPEEALLNTLTKDDILKAIEDLPVEFRTVVVLVLVEGFSYKEAAEILDIPIGTVM
                     SRLWRGRRLLQKSLWEYSTRKNDSERKNQEGWTAKKS"
     gene            complement(10914..11219)
                     /locus_tag="PK016_05725"
     CDS             complement(10914..11219)
                     /locus_tag="PK016_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02602.1"
                     /translation="MQELPLKCKNCKALITLEDVTEIGIYKIPAYRGSGYIRYICPNC
                     KDFGEVNFNLEKLEDVARITTKKKKKTKPKGQITIDEIIQFHKELGNLRNADFLKDS"
BASE COUNT         3380 a         2601 c         1927 g         3377 t
ORIGIN      
        1 gggggaggac agcatccccc ttatagccat ttagaaaaag aaaaaacaca aaagcgcaga
       61 aagactactc ggggggaaat acaaacatac ctctataacc cctataatct cccttaaaaa
      121 ccaaaatata gcgagagagc agaaaacgtc ctccaccgat atcataattt tacagttaac
      181 tgcaaagggc tatagctcta actggagaac cggtacaccc ctttagtttt aaggggaatg
      241 cagcaaaagt tactcttttc ccccggagct cttccaggtt ggttaaagat tcatagatga
      301 tgatttcttg agccaaaaga aggcgatgaa ctacaaatgg ttgggaatca atgctcgggc
      361 tatcagttcc cacacctttt acttttttgt ctctcaaaaa ttcagctaca ctctcggtga
      421 gataaactcc ctgagtgaag tattctggcc tccccagtag tttatctaca ccactagaga
      481 aaattacgat gtctccttcc tctatatctt gtcctcctaa aacccttttc aggtcagaaa
      541 gctctaattt acccttagaa agataggaag aaacatctaa gaccagagct tctccccaga
      601 aacactccag tgatattttt tctactgttt tccctccttc gataaaatga gcaggagcat
      661 caagatgagt cccggtatgc tccaccatga aaaaggcatt ggtagcattt ccatggactg
      721 ccaaattact ccagggtaaa aaagagagag gaggttgccc aggaaaaact ggaatatcag
      781 atgtaagatt gagagtcaaa tcataaattt ttttcatttc tgttcacaga ggaaaaatca
      841 atttatgaaa acgaccacaa taaaaaacat aactggcaag aattcactta cttttatact
      901 acgccatgac accgtctttt tcttcctctc ctgttacctt agaataaccc tctattcgtt
      961 tcttataaag aaaaaaagtc tgcactatct ccttatacat cttaatccgg tggtaaagtc
     1021 ctggaaaaaa accatatttc tcttccttat gataatgagc taaaccagtg aggggaacta
     1081 gctctatatt tactttattt tcccgggcaa tcctggtcag aaaaacttcc actccaaaac
     1141 gagtccagga aaaatcaggt aaatttctta cccactctcc ccgcacccct cgcattccgt
     1201 tgagaatggg aaacagcttt tgagccaggt ctccccatct ccgaccttct tcaaaaaccc
     1261 caatcgtcat aactaaagat tgatcctcta ttaaggggtt aaccaaagct aaaatatgtt
     1321 cctcagtaag agcaaccaaa tcggcatcca aaaaaacata aatgtcactg tctttctttc
     1381 gccgaatccc ctctactaaa gctgctccct tccctctatc actatctaga taaattacat
     1441 ccccaggaaa ctgacgagcc acatcctgag tcccatcctg agaatggcta tcgatgacca
     1501 ctatttcatc tacccattcg atcttgctga ctacctccaa caccttaggt aacctaggag
     1561 cttcattgta agcgggaatt aaaatggtga ttttttgaga ttctaacatt gtctctccat
     1621 tcattttttt aaagttttta tcttctctct tggaaattcc ctcttttgca tttacaataa
     1681 tattatcaca taaagtagga aaagggggaa aaatgaataa ttcagaagaa ggaaaaataa
     1741 ttagaaattt ttactatctg gttctcatta tcattactct atatctcact tacatattaa
     1801 gagatgtttt aatacccttc gttttgggtg gacttttagc ttatgctctt tctccggcag
     1861 tgcaattctt gaattctcga ggactttctt ggaagctttc agtaattata atatttatag
     1921 tagtattagc atttttctta tccctttttg tctttctagt ccctgaagta ctttctcaat
     1981 ttagaactct aacctcccaa tggtctcaat actcagaaag tttaatcgac aaaatattgg
     2041 aaggaataga gaaattaaat gaacgccatc ccgattccaa catttctcaa accatagaaa
     2101 gttttctaac caccatttct actaatcttc aaaattattt aacccatata atcagcggta
     2161 tccctaactt ggtatccatt ttggctaata tcatcttttt aggctttatt atgacccctg
     2221 tggctcttta cttttttatg gctgatgcta ccaaagttcg ccgctctttg gtaagaatgt
     2281 ttcccttttc tcgccgaaga gaatatatag ccctatttcg agaaattgac caggtcttag
     2341 gaggttttat aagagggaga gccttaattg ctctttttgt gggaatatgt gctactgttg
     2401 gtttagccct aatgaatata gactttgctc tggctatagg agtgatcgct ggaatagtag
     2461 aaatagttcc ctatctgggt ccagtagttg gagctatacc agccattatt ttagcggcca
     2521 ccaaatcacc atggctagtt ttaggagtag cacttctctt cggaggtata aatttcgtag
     2581 aaggaatatt tatatctccc aagctcttag gtaaagaaac cggcctccat cccctaaccg
     2641 ttctctttgc tcttgcagtg ggaggaaagc tttatggagc attagggata atcattgcca
     2701 taccagtagc tggtgtttta aaaggattac ttttccacta tcggaaaaat aagaaactct
     2761 cttcaacttc ctgaatttta catagctttg tcaaggtcat ccaaaatttc accagccttc
     2821 cctcgcaaaa taaaacgagc gcggtggtca aagggggttg gttccagatt aactatgata
     2881 aggtaagggc tatatgaagg aaggaagttg gctggagaaa cttgcaagct agagccaatg
     2941 accaaaagag gaagacgatg agcaagctcc actgcttctt caaaacaagg aggtaacata
     3001 tccccaaata gcaccacatc agggcggata atcccaccac agtggcaatg aggtggtata
     3061 tctccttcat ctactttttc ttttatggcc tccataggaa aggaagtctt acatgtgagg
     3121 caatggccag aacgaaggtg accgtgaatt tccagcacct tttgagaacc agctttataa
     3181 tgcaaactat ctatgttttg agtaactacc gcttctacca gacctttctt ttcccactca
     3241 gccagtatgt agtgagcttt attaggctga gcgttttcaa atttcataag aaccggaaga
     3301 ccttcctgat aaaaaatctg aggtctatta aaaagaacat cagtagataa aatctccata
     3361 gggtcatatt tttcccacaa accgctcccc gggcttctaa aatcaggaat accactttct
     3421 gtagatattc ctgccccggt gagcactaac cagggttggc gctctaacat aaaagatgcc
     3481 gcttttttaa ttaaactttc atccattttt ctctccttaa actatacttg atacaataat
     3541 aaaagtaatt accatagaca tcatgcttcc ggccacaact tgatataaat tatgcttacc
     3601 taaataaatc cttgcccagc ctaaaggtac aagtaaaaga taaagaaatg cccagtaagg
     3661 gttaagaatc ataaccaggg cagtgacagg gccagcaatg ccagccagat gaagactaat
     3721 tttccagaaa agagaaataa gtgctcctac aaaagttact cccagataac aacaggtaat
     3781 ggccacaaga ggcaaaggtg cagaagcaag aagaaaaata accaaagcta aaaggtaaga
     3841 actgcaaatc attaaataag ggaaaaatcg ctcttctcta ttgggtaaat cagggtctga
     3901 tacttttccc tttttagtta aaaagagaac caggagtatg ggaaacacac ccacaaaaaa
     3961 cagaccaatt aaaaagaaaa cccaggttct ccaacctgtg gcaaaactaa agctcaccac
     4021 cagatataaa agcgttgcta tagcactggg ctgtagcact aaagagaaag tttgagctaa
     4081 actttctttt ttcatttttc cacctcaatt ttctcgtaca ttatatttat tcatagctct
     4141 ttcccatcct ttctccaaaa tagaagaaag agcagaaaca gctttctcct ctccctgctc
     4201 taagagaatt ttctcctctc cttccgggat tcctaaaaca taatccatgg ctttttcccg
     4261 aaaaggtggc ttacctattc ccactcgaat acgcaaaaaa tcatcccttt tcaacgtttc
     4321 tactaaagaa ttcagccctc gatgtccagc agtacctcct ccttttttga ttcgcaccac
     4381 tccaggagga aaatccatct catcgtggac tacaatcaat tctccttcaa aaaaaagctg
     4441 aaatcgggga aaatagagaa gcccctcccc gctttgattc atataggtaa ggggctttaa
     4501 taaataaact ttctttcctt taacttcccc ctctgcccag ctcaagcgat gaccgatatt
     4561 tatccacttc cagtcatatt gatgagaaaa acggtctacc actcgaaaac ccacattatg
     4621 ccgggtgagc gagtatttct ctcctgggtt acccagacct attattaaat acactgacta
     4681 cttttcttcc tcagtttttc ctttccctat tacctggggt tcttctactt ctcctttagc
     4741 ttcttcccct atcttttcct ccaccacttc tactttagga ggaataacca ctactatcac
     4801 ttcatccggg tctttcatca ctgtgacatc ttgaggaact tttaaatctc caactcggat
     4861 ggtgtctcca acttccagct cactaacatc cacctcaata tcttcaggta tagcaccact
     4921 agggcattct acctctaact ctggagtaac taattccaaa gtcccaccag cttttactcc
     4981 tggagcttct ccttttattc tcaccggcag tgaaagagtt attttttgac cggtttgcac
     5041 ctcataaaaa tctacatgcc agatttcttc ttttacagga tttttctgca aatcacggat
     5101 aatccctttt ttcatttctc cctctaaaga taaatccagc atatggtggg tgatacctgg
     5161 tatctgcaaa atgcgcttca attctttctc tctcaccttc accagctttg cttttccatc
     5221 cttgctatct cgagaataca agaccgccgg tatccagcct tcttttctca ttttgcggct
     5281 tttttctttg cccttttctt ctcttctttc cagattaatg attacgttat ttcctttagg
     5341 catattacct tacctcgctt tttcatataa aaagttcact gactgacaat tcaccatgaa
     5401 tccttttaat tgcttcagca aaaataggag ctacagaaag tacttttatt ctgttcactc
     5461 cttgttgaga aagtttttcc tggtctactt taatagtatt ggtaaccact acttcctgaa
     5521 ggcaggattt ctcaattctg gtaaaagctg gctcactaaa aacaggatgg gtagcacagg
     5581 catagatttc tttagctccc cgtttatgca gggcttcagc tccttccacc agagttccgg
     5641 cagtatcaat aaggtcgtct acgataattg ctcttttatc tcttacttct cccaccacat
     5701 tcatcaccat cacctgagaa taagtaggcc gatgtttgtc aataatcgct aaatctgctc
     5761 ctaattgatt ggcaaaagcc cgagctcgag ctgctcctcc tacatcaggg ctaaccacta
     5821 ctacttcttt ccgcccccga gagagaaaat aacgagccag aagagactga gcagccaaat
     5881 tatctactgg tatatcaaag aaaccctgaa tctgtccggc atggagatcc atagttaaaa
     5941 tccggtctac tccagcagta accaagagat tagctaccaa ttttgccgaa attggttctc
     6001 gacctttagt tttgcggtct tgtttacagt aaccataaaa gggaataacc gccgtaactc
     6061 tttctgctga agcacgatgc aaagcatcaa tcattattaa aagctccatc aaatggtcat
     6121 tggccggagg tgaagtgggt tgtatcacaa aaatatcaca acctcgaaca ctttcttcaa
     6181 tttgcactct tatttctcca ttgggaaaac gtcccacgtc tgcttttcct aaaggaatat
     6241 caagatacca gcacacctcc tgagcaagaa gtggattagc tgtaccagaa aaaactttta
     6301 gatgacggga aaattcctgg ccgttgaaca tttatttttt attcctccgt atcttccttt
     6361 ttacccaatc ctcaatgttt ttctgcttag ctctccctat ccccagagaa taaggaggaa
     6421 catcttgagt tatagtagag ccagctgctg tataagcacc tttcccaata ctcaccggtg
     6481 ccactaaaga attattgctt cctataaaaa cttcatcttc tataaaagtg ggatttttct
     6541 tctccccatc atagttacag gttatagttc ccgctccaac gtttactttt ttacccactt
     6601 gcgcatctcc taaataactt aaatgcaaag ctttagttcc ttctccaacc tgagaatttt
     6661 tcacctctac gaaattaccc acctttactc cttgttccag cttcgtaccg ggacgcaaat
     6721 gagagaaagg acctactctc acttcttctc ctataaaaga cccttccacc acactatatt
     6781 ctacagagct atttttaccc actctgcttt ccgatattcg agaaaaagga ccaattcgag
     6841 ctcctttttc gattatgctt ttcccttcta taactgtccc gggatataac cacacatctt
     6901 cctctaccac cacatcccaa tcgatataag tagcagaagg gtctattatt tttactcctt
     6961 cgtcccacag ggaagcgatt tttctctcct ttaatattaa gatagcttta ctaaaatcag
     7021 caggggtatt aacattcaaa aattgttcat cccactctac cacaaaagcc tctggtcggt
     7081 atcctttttc tcgagtcact tgaactgcat cagttagata atactccttt tgagcattac
     7141 ggttgccaat cagagagata atttctcgaa aagcttctcg ctgaaaggca aaaatcccta
     7201 aacttacctc ggatattttt ttctcccgtg cggaacaatc tttttcctct acaatcccca
     7261 gaggttctcc tttttcattt cgcaccaccc gtccaaagcc ggccgggtga ggagcaatag
     7321 aggtcaaaaa agaaagaggg gaatgctttt cttctgcttg agagagaaaa gaaaaaatag
     7381 tctctccccg gagaagaggc atatcagcat aaagcactaa aaaccagtca atgccttctt
     7441 gaattaaagg aaaaacagta ctaacagcgt gagcagtgcc ctggggctct ttttgaacta
     7501 ctatggtcat ttcctgacca aaattgcttt tcatcaactc ttccatagag ggagaaacta
     7561 ccagaaatct ttgcccgaac tctccccact tgagctcatc cagcaaatat tgcataacag
     7621 gtttgcccca agcaggtaga agcaccttag gaagagaaga gcgcattctt ttaccctggc
     7681 cagcagctaa aactatcaaa ccccatcgag aaagattcat atttttcacc acaccagata
     7741 aaacgtgacc aaaccacgcc cgatttatag aagctattaa ctattttatc aaatttctat
     7801 ccttaatcaa ctccactgaa ttcccggaga gctattacac ctgaaattaa cactatctaa
     7861 caaagatgga gagcctcaaa aaaccaataa cagaaggaaa acaaaaacat tctaccacag
     7921 attgatgtta tctgagtttt taaaaaattc tgataaattc tatatgcact acgattaaag
     7981 attggccttt tctggtacaa tatagaaaat tctgagggag gtgcaccgtg gtaaaactgg
     8041 ccaaaagtat gaaagaggtt attaaccagt tagctcaaca aatcatatct tctactgctt
     8101 ataagaattt ccaggaaaaa agagcccttt tagctaatca ccctgagttt ctctctcttt
     8161 ttcaacagct ggaggaaaag caaaaagagc tggataaatt aaccgataac gaagaagata
     8221 tacctgatga atatttagaa gaattagaag atatcagtaa acaattactt tctgaacctg
     8281 tgtttgtaga ttatcttaaa gctcaggatc agttagtgtc tctcctagag ttaattaatg
     8341 atgaactgag ttcaactctg ggttttgact ttgctgactc ggttaacttc ttaagagaag
     8401 aggaagaggg agaagaatcc tgggaataag tttcttctaa cttgaggagc gaatacctta
     8461 cccttcccat atcaggcaaa tggtcgagag aagttttgtg gttgtcaaac tgaaactgac
     8521 gacagctatc gggaggggta aaagaatcag gaattttaat gccggcatat aaagtgttcc
     8581 atcccatttc ccgcaaacgt ttagcggtag aaggttcaca cagttcttct atgggttgat
     8641 ttttctttat taccgtgcac tttccagtgc ttatgtctac tcctccacca agaaaagcac
     8701 tggtagcagg ttcaaaaaag agacaatatc ggcatgtgtc aagacgagta tggcatttgg
     8761 ggcagtaaag cgggcgactc ttcactgtta ttccctcgtt ggggatagtt ttaagagttc
     8821 caggaatttc taccttacaa aaagggcaca aaaacgaaga aggcaaggta gccttggtaa
     8881 ctcttgatga ttctttaagg atttggtttt tttcctcatc actaagagac agatgtggtt
     8941 ttctgaccca ttgaaactta ggataatgga agaaagcctc tatcccataa agtaaagacc
     9001 ggaaaaaagg aactcctttc ttccgggaaa aagaaaaagc agaaacagca taataaaagg
     9061 aaagagacaa ccagaaaaca agccagacat acagaactac cacttttaaa acaggagagg
     9121 gaaagagaaa gtataatata cctccaccta accagatgag aagaaacata aggtgatgat
     9181 gagcactcat aatttctggt tttctgatat tttgagacga ggctcctcaa tagctacctt
     9241 ggtataagga gggatagaat gagtaagcca gacattccca cctactacgc ttcctttccc
     9301 gattacggtt tgacctccca gtatggttgc tccagcataa atggttacat catcttcaat
     9361 agtagggtga cgtttggttc cccgaataat tttacctcgt tcgtctttgg gaaaagagag
     9421 agctcccagt gttaccccct ggtaaatttt gacattattc ccaatttcac aagtttctcc
     9481 aatcaccact cccgttccgt ggtcgatgaa aaagctccga ccaatcttag ctccgggatg
     9541 gatatctata cccgtcactg agtgagcata ttcagtcatc attcgaggta tgagaggaat
     9601 cccttgaaca aaaagctcgt gagccaggcg atagatagaa atagccacaa gacagggata
     9661 actaaatatt atctcatcca gacttttggc tgctgggtca ccatcaaaag cagcttgaac
     9721 atcatcttcc aacatctccc gcaccgcagg gagtttacca aagaatattt ctacttcatg
     9781 atgagctttt tcttccccct ccggggatct gtcacccagg gaaaaaataa tttcctggct
     9841 caattcacca aagagatagt ctaatttacc ccccacaaaa tactcagtgt tgagccagca
     9901 tagattagtt cttcctaaat aaccagggaa aagcaattcc ctcaattctt cagaaatttc
     9961 tatcacttta gcccgggagg gaaaatgctt tttttctatg ttggttatct tttctgtttt
    10021 tcggtagctt tctaccagag acttagccca tctttcgatg gctataggat gagaggaagt
    10081 caatttttat tcctcgctta gaaccgggcg atgacattct ctattctgaa aaacaaactg
    10141 ggagggactt catcattacg cccatttatt ctaatcaaat tctccagatt tttctccaac
    10201 tcgtacattt ggcagcattt gaagcaccat ttcagatgtt cttcaatctg ctgtctttca
    10261 cgttcagtca atatctcatg gtctaaatac aagtataatt tttctaagac ttctttgcag
    10321 tccatccttc ctggttcttc ctttctgaat catttttccg ggtggagtat tcccacagcg
    10381 atttttgcag taatcttcta cctcgccaca aacgagacat aactgtccct ataggtatat
    10441 ccagtatttc tgccgcttct ttataagaaa aaccctccac caagaccaag accaccacgg
    10501 ttctaaactc tacaggtaag tcctctatag ctttaagaat atcatctttg gttaaagtat
    10561 ttaacagagc ttcttctgga cttattccct cagctatagg ctggaaaaaa gattcttcct
    10621 ccatttcatc taaggaagga agcttttgtc tgtctcttac ccggtagtag ttgtttataa
    10681 aagtgttagt caaaattcgg aatatccagg cccgaaaatt ggttccttcc tcaaatcggt
    10741 caaaggcagc aaaagctttg gttatcgttt cttgaaccaa atcttcagca tcttcccggt
    10801 tacgagtcat acgcagagca gtgcgaaata atgcccctaa ctcgggattc agtaactctt
    10861 gaaaatactc tttttgttcc tctttggttt tcaccattac caatcaacct ttttcaggaa
    10921 tcctttaaaa aatcagcatt tctcaaattt cccaattctt tgtgaaattg gattatttca
    10981 tcaatagtta tttgaccttt aggtttggtc tttttcttct ttttggtagt gattcgtgct
    11041 acgtcttcca gtttctccag gttaaaatta acttcgccaa aatctttgca attgggacat
    11101 atatatctta tatatccgga gccccggtaa gcaggaattt tataaattcc aatttcggtt
    11161 acgtcttcca gggtaatcaa cgctttacaa tttttgcatt ttaaaggaag ctcctgcatt
    11221 tcttcctcct aaactatttt tgaatatttt accaagagat ggtaaataat acaagaacat
    11281 ttttt
//
LOCUS       DAOSVE010000055        11284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_3253_length_11284_cov_73.491941, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000055 DAOSVE010000000
VERSION     DAOSVE010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 11284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11284
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3253_length_11284_cov_73.491941"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1754)
                     /gene="uvrB"
                     /locus_tag="PK016_05730"
     CDS             complement(<1..1754)
                     /gene="uvrB"
                     /locus_tag="PK016_05730"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HXL02603.1"
                     /translation="MKRGSFHLSSLYEPCGDQPQAIEQLVDGLKKGYRYQTLLGVTGS
                     GKTFTMANVIAQIGLPTIVISHNKTLAAQLYSEMRAFFPDNAVEYFVSYYDYYQPEAY
                     VSEYDLYIEKDASINQQLDRLRLKATSSLMERRDVVIVASVSCIYGIGSPREYEKRIF
                     HLRVGQEWKREELVKRFIDLLYERNEIEFSPGHFRMKGDTVEIYPAYADTALRLEFFG
                     PELESIKVFEPVSGKTLERLEEVFIYPAKHYLVDESEFERALQNIEREMEERVNFFLS
                     AGRLVEADRLLRRTKYDLELLRETGYCPGIENYSRHLDGRKPGEPPYTLLDYFPSDYL
                     VIIDESHVTIPQLRGMQEGDRSRKESLVNFGFRLPSAFDNRPLTFEEFENKVKRCIFV
                     SATPSAFELQHSEQVVEQLIRPTGLLDPQVEIRKARGQVEDLLGEIRKVVEKNQRVLV
                     TTLTKKSAEDLCDFLYGMGVKVRYLHSEIDTLERAKIVRDLRRGEFDVLVGVNLLREG
                     LDLPEVALVAILDADKEGFLRSEVSLIQTMGRAARNVEGRVILYADEMTGSLERAVEE
                     TRRRRKVQEEYNREHGIIP"
     gene            complement(1811..2398)
                     /gene="coaE"
                     /locus_tag="PK016_05735"
     CDS             complement(1811..2398)
                     /gene="coaE"
                     /locus_tag="PK016_05735"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888955.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HXL02604.1"
                     /translation="MPRVWAIGGGAAAGKSTVASILREKGIEVVELDKLSRELSKKGG
                     PLWKNIVKTFGSQFLTEQGELDRTKLGRLVFRDWKSLFSLNSVTHPVLLQEVRKKLAS
                     LPPSQIVAVEGAVLFEAGFLPLWSKLIFVEADEAIREKRLLKTTGLKAREIKDRLRSQ
                     RFLNCLKRRADFILKNEGSLEELEETIEDWLEELK"
     gene            complement(2408..3832)
                     /locus_tag="PK016_05740"
     CDS             complement(2408..3832)
                     /locus_tag="PK016_05740"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HXL02605.1"
                     /translation="MEEVVLVATEAYPYAKSGGMGDVIGALFKYLPDYGFPVTLFLPA
                     YQGMVDNYPFSPEREVDFYFGDTMVTAQFFKMEEGKRKIIAVQGRDFFSRERMYGYSD
                     DLERFIFFSRAVFEYLVRWQEGNFLLHTHDWQSALLCAYIVKYWPSYRPKPEKIVFTI
                     HNLAYQGIGRGDLYRLINLPGQFFTYEYLEFYGNLNLMKAGLIFSDVITTVSPTYARE
                     ICCPEFGEGLDGVLRAISQKKKIVGIVNGIDKEVFNPATDSLIPANYDLSSREKKVIN
                     KEKLIREFFPKAKDLSLPVISFVSRLVEQKGVDLLLHDPNFFFNLPAYWFFLGTGEEI
                     YEKALKDIEKKHPRVKATIRFDDELSHLVYAASDLLLLPSRFEPCGISQMIAMNYGTL
                     PLVRRVGGLIDTVIDYPFNPCLSTGFQFENYSFSDLSKALTRALNTYFENKELWNTMV
                     VNAMQCDFSWKNSLPRYLEIYRER"
     gene            complement(3859..4773)
                     /locus_tag="PK016_05745"
     CDS             complement(3859..4773)
                     /locus_tag="PK016_05745"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HXL02606.1"
                     /translation="MEDKKLVVIDGSSLIYRAFYALPRLTTSSGLPTGATLGFLNMVL
                     KLLDDYRPFNVVVAFDHPQKTFRHELQESYKAQRKPMPDELKPQIGIVKELLELMGFV
                     ILEMPGWEGDDLMGSVKEKVKSDFQVLLVTSDLDMLQLLDERTVLLQPHRGVTSLKKI
                     DLEEFQKEWGISPAQWVDVLALSGDPSDNIFGVPGIGEKTAVKLILQFGSWEGILNHL
                     SLLPPKLREAIESNRDKVEENRKLIVLEKDLPLDLEIQAWGWSKVDKNKLFQKLESLE
                     FRKIKERLVKNNRVNSGSLFSHWDEEKE"
     gene            4830..4917
                     /locus_tag="PK016_05750"
     tRNA            4830..4917
                     /locus_tag="PK016_05750"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4864..4866,aa:Leu,seq:caa)
     gene            complement(4920..5756)
                     /locus_tag="PK016_05755"
     CDS             complement(4920..5756)
                     /locus_tag="PK016_05755"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXL02607.1"
                     /translation="MKNKIGQLVIIGWLILGVVPGIFAQTKEECYQNGYIYFSQGNYE
                     EAEKWYRKALEIDPNFWEARYWLGKAEEQLGKIEEALKEWTVVLMAQPDNRDVFQKWR
                     YYAPSYVSLGESDIQRLKSIFLEGGSPSFSEEEAWQEVMPQAFLLLERKDFLSPYLGA
                     KIMRWGGRNLSSLFYSYERRGYERALDNLLLNLDEDQELVYQFVRECEERFGEDQVFQ
                     EKLSLLYEKVFAQQLDLPPEEVEGMRKIELQVEGREVKVSAPQGEEDALLHPDTGFYL
                     GE"
     gene            complement(5753..9160)
                     /locus_tag="PK016_05760"
     CDS             complement(5753..9160)
                     /locus_tag="PK016_05760"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HXL02608.1"
                     /translation="MSFSHLHLHSEYSLLDGAIRIDDLLNKVKSQGMEAVAITDHGVM
                     YGVVDFYAQAKKAGIKPIIGCEVYVAPGSRFEKTVVRGAESAYHLVLLAENQEGYKNL
                     VQLVSKAFLEGFYYKPRIDKALLREHSRGLIGLSACLAGEIPSLILSGEREKAEKVAL
                     EYAEILGKDNFFLEIQENKIPEQRIVNQALLEIGNKYDIKIVATNDCHYLEAEDASVH
                     DVLLAIQTATTLDDPKRLRFPTAEFYFKSPQEMERDFSYCPQALENTSLIAERCQVDL
                     ELGKINLPEFPVPPGLDAPGYLRSLCLQGLVNRYGDPPPERAREQLEYELKVIEDMGY
                     STYFLIVWDFVNFARQKGIMVGPGRGSAAGSIVSYTLGITSIDPLQYGLLFERFLNPE
                     RVSMPDIDIDFCFERRGEVIEYVAEKYGKTNVAQIITFGRMMARQAVRDVGRALGWSY
                     GEVDKIAKLIPGGPGVTLKKALESNSQLRELSRQNKELQHLLEVASRIEGMCRHASIH
                     AAGVVISNQPLTDYVPLQKMGEGEIVTQFEMDALQELGLLKMDFLGLRTLTVIEKALQ
                     IIRNTRHQEIDLEKIPLHDEETFSLLSKGETTGVFQLESSGMKELLKRLNPSNIEDLI
                     AILALYRPGPLGSGMIEDFINRKHGKVKIEYPHPALEAILRETYGVIVYQEQVMKIAA
                     ELAGFTLGEADILRRAMGKKQPEVMKEQRAKFIEGALKRGCSKEKAEEIFDLIEYFAG
                     YGFNKSHSAAYALISYQTAYLKAHYPAEYLAACLTSVANDNEKVAKFIGECKRLGIQV
                     LLPDINESLANFTVVGENKIRFGLAAIKNVGGAAVEEILTRRKEGKFLSIFDFVERVG
                     SRVINKRVLESLVQAGAFDELHPHRAQLLASLEDIALLSRKNKSKKKQKSLFGEENNN
                     GESFSLREVEELPREELLRMEKEMLGLYISDHPLKVESFSSSWSAVPLGQIPHLKQEV
                     KVRIRGIVNKSKKITTRNGGNMYFVTLEDETGSIEVVFFPQLALQLEDYLSRKQVVWV
                     TGKVDLLDNGEHKIIAEELIDIEEKKNASIVHIAIPEEGDTLKTVYFLKDYLRRFPGS
                     FPVVLHIRGEKESWAVELGESFCIDWNREVERMIKDSLEKGEIWLAG"
     gene            complement(9163..10608)
                     /gene="gatB"
                     /locus_tag="PK016_05765"
     CDS             complement(9163..10608)
                     /gene="gatB"
                     /locus_tag="PK016_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HXL02609.1"
                     /translation="MSDFTTTIGLEVHCQLLTQAKMFCHCSTDYIGKEPNTLVCPVCL
                     GLPGSLPVLNQKVVELALRAALALHSQILPQCVFHRKNYFYPDLPKGYQISQYDLPIG
                     VGGFLEFYSQGVKKRVRIRRVHIEEDAGKLIHEGVSLPPGTSGVDCNRCGIPLAEMVT
                     EPDLSSPEEARDCLMMLRNIVCYLGVSDGNMEEGSLRCDANISLSSSSLLGVKVEVKN
                     MNSFRAVYRALSYEVERQKKLLEEGKPIFQETRHWDEAKEITVSARGKEEAEDYRYFP
                     DPDLLPFSIKEEMIEEIKSELLELPLEREERFMTEYELSQGEAEILVQDKEIADFFED
                     TLRDFDEPRMVCNWITTEVFKNLRELGATISQAKITPAHLAELLKMVAKKEISGTIAK
                     SVLEEMFATGARAREVIARRGISFITDEKELENIARDVIERNPQSVADYLSGKEKALQ
                     FLIGQVMKETRGQADPELTRNVLIKVLSSEE"
     gene            complement(10601..>11284)
                     /locus_tag="PK016_05770"
     CDS             complement(10601..>11284)
                     /locus_tag="PK016_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HXL02610.1"
                     /translation="PREYFPSGLEKAIGEKIQEALEKLRGEGIEIVEVSLPHTDYALE
                     SYYIVAPAEASSNLARYDGVMYGLRVEGDNLQDMYFKTRTLGFGKEVKRRIILGTYCL
                     SSGYYDEYYLKGMKVRTLIKQDFEEALKNCDLLITPTTPSFPFFLGKEVKDPYEMYLN
                     DVFTIPSAMAGLPSVSLNCGYKEGLPVGLQIIGGPFEEGKLLGLALFLEKLLALPSPF
                     PGFKEDNNE"
BASE COUNT         3165 a         2893 c         1994 g         3232 t
ORIGIN      
        1 ggtattattc cgtgttcccg gttatactct tcctgaactt tccgccgacg tctggtttct
       61 tccaccgctc tttctaaaga accagtcatt tcatcagcat aaagaataac cctaccttct
      121 acattgcgag ctgctcgacc catagtttga ataagagata cttccgagcg caaaaaccct
      181 tctttatcgg catccaggat agctaccagt gccacctccg gtaaatctaa tccctctcgc
      241 agcaagttaa ctcccaccag gacatcaaat tcccctcttc ttaagtctct aacgattttt
      301 gctctttcta aagtatcaat ttcagaatgg agataacgta ctttcactcc catcccgtaa
      361 agaaaatcgc acaggtcttc agcgctcttt ttagtgagag tggtcacgag aactctctgg
      421 tttttctcca ccacttttcg gatttctcct aataagtcct ctacttgccc tcgcgctttc
      481 cttatctcca cctgagggtc taaaagaccg gtgggtcgaa tcaattgctc tactacctgt
      541 tcactgtgct gcagctcaaa agcagaagga gtagcagaaa caaaaataca ccttttcact
      601 ttgttttcaa actcctcaaa agtaagggga cggttatcaa aagcggaagg gagtcgaaag
      661 ccgaagttca ccagagactc cttccgggaa cggtctcctt cctgcattcc ccgcagctga
      721 gggatggtaa cgtgggattc atcaatgata accagataat cagaaggaaa ataatcgagc
      781 aaagtatagg gaggctctcc tggctttctt ccatctaaat gacgggagta attttctatc
      841 cccggacaat aaccggtctc tcgcaaaagc tccaagtcat atttagttct tcttagcaat
      901 ctatctgcct ctactaagcg ccccgcagag aggaaaaagt ttaccctctc ttccatctct
      961 ctttcaatat tttgaagagc tcgctcaaat tcactttcat ccaccagata atgcttggcg
     1021 ggataaatga atacttcttc cagccgctcc agagttttac cactcactgg ctcaaacact
     1081 tttatagact ctaactccgg gccaaaaaat tccaggcgca gagcagtatc ggcataagca
     1141 ggatatatct ctacggtatc ccctttcatc cggaaatgac cagggctaaa ttcaatctcg
     1201 tttctttcat agagcaagtc tatgaaacgc ttaaccaact cttctctttt ccattcctgc
     1261 cctaccctca ggtgaaaaat cctcttttca tattcccggg gagaaccaat accataaata
     1321 caggagaccg aagccactat cactacatct cttctttcca taagagaaga ggtggctttc
     1381 aaccgcaggc ggtccaactg ctgattaatg gaggcatcct tttcaatata gaggtcatat
     1441 tcagagacat aagcctcggg ttgataataa tcgtaatagc taacaaaata ctctaccgca
     1501 ttatcaggga aaaaagctct catttcactg tagagttgag cagctaaagt tttattatga
     1561 gagatgacaa tagtgggaag gccaatttgg gcaataacat tagccatggt aaaggtttta
     1621 cccgagccgg taacccccag caaagtctgg tatcgatacc ccttctttaa gccatccacc
     1681 aattgttcaa tagcctgggg ttgatcccca cagggctcat aaagagaaga aagatggaaa
     1741 ctacctcttt tcattggtct tattactcct tactattttt cctccaatta aaaacccatc
     1801 agaggatttt ctatttcagc tcctccaacc aatcttctat tgtttcctct aactcctcta
     1861 aagaaccctc gtttttcagg ataaaatctg ctcttctttt caagcaattt aaaaatctct
     1921 gcgaacgcag acggtctttt atttctctcg ctttcaatcc tgtagttttc aacagtcttt
     1981 tttcccttat agcctcatcc gcctccacaa atatcaactt gctccagaga ggaagaaaac
     2041 cagcctcaaa aaggaccgcc ccctcaactg ccactatctg agaaggagga agggaagcta
     2101 acttttttcg cacttcctga agaagaacag gatgggtaac gctattgaga ctaaaaagac
     2161 ttttccagtc ccggaaaacc aaccttccca gtttagtccg atccagctct ccttgttcag
     2221 tcaaaaactg cgacccgaaa gtctttacta tgttcttcca cagaggtcct ccttttttag
     2281 aaagctcccg gctcaactta tccagttcca caacctctat tcctttttct cggaggatag
     2341 aagccactgt gcttttacct gccgcagctc ctcctcctat agcccacacc cgaggcatag
     2401 gtttctccta cctttctcgg taaatttcta aataacgagg aagagaattc ttccaggaaa
     2461 aatcacattg catagcattc actaccatag tattccagag ctccttattt tcaaaatatg
     2521 tattcagcgc tcgagtgagt gccttagata aatcagagaa agaatagttt tcaaactgaa
     2581 aaccggtact gaggcaagga ttaaaaggat agtcaatgac agtgtcaatt aatcctccca
     2641 cccgacgcac gagaggtaac gttccataat tcatggcaat catctggctt atcccacagg
     2701 gctcaaaccg ggaaggaaga agcaacaaat cagaagcagc atatactaaa tgggacagct
     2761 catcatcaaa ccttattgtc gctttcactc tgggatgttt cttttctata tcttttaaag
     2821 ctttctcata aatctcctca cccgttccca ggaaaaacca gtaagctggt aaattaaaaa
     2881 agaaattggg gtcgtgcaaa agaaggtcta ctcctttttg ctctaccaac cgggaaacaa
     2941 aagaaatcac cggtagagaa aggtctttcg ctttcgggaa aaactcccga attagttttt
     3001 ctttgtttat aactttcttt tcccgggagg ataagtcata gtttgccgga atcaaagaat
     3061 cagtagccgg gttaaagacc tccttatcta taccgtttac aatgcccact attttctttt
     3121 tctgggaaat agcccgtaat actccatcca atccttctcc aaactcagga cagcatattt
     3181 ctcgagcata agtggggcta actgtggtta taacatcaga aaatatcagt cccgctttca
     3241 tcaaattcaa attaccatag aactccagat attcataagt gaaaaactga cccggaaggt
     3301 taatcaaccg gtagaggtct ccccggccaa taccctgata agctaaatta tggatggtaa
     3361 acactatctt ttctggttta ggacggtaag aaggccaata ttttactata taggcacaaa
     3421 ggagagcact ctgccaatca tgagtatgaa gcaaaaaatt tccttcttgc catcttacca
     3481 agtattcaaa aaccgcccgg gaaaagaata tgaacctttc taaatcatcg gaataaccat
     3541 acatcctttc ccgagaaaag aaatcccttc cctgtacagc tataattttt cgttttccct
     3601 cttccatttt gaaaaactga gcggtcacca tggtatcgcc aaagtaaaaa tccacctctc
     3661 gctccggaga aaagggataa ttatccacca tcccctgata ggcaggaaga aaaagagtaa
     3721 caggaaagcc ataatcgggc aaatatttaa acaatgctcc aataacatct cccattcctc
     3781 cacttttggc ataaggatag gcttcagtgg caaccagtac tacttcttcc atactcatcc
     3841 tcccttaaaa ttaatgtatt actctttttc ttcatcccag tgagaaaaaa gagaaccgct
     3901 attcactcgg ttatttttca caaggcgctc tttgattttc cggaactcta aactttccag
     3961 cttctggaac aatttgtttt tatccacttt tgaccacccc caggcttgga tctccaaatc
     4021 taaggggaga tctttttcca gaacaattaa ttttctattt tcctccactt tatcccggtt
     4081 actttcaatg gcttcccgga gtttaggggg taaaagagaa aggtggttta aaatcccttc
     4141 ccagctaccg aattgcaaaa tgagtttcac tgccgttttt tctcctattc ccggaacacc
     4201 aaaaatgtta tctgaagggt ctccactcag agctaaaaca tcaacccact gcgccgggga
     4261 aataccccac tctttctgga actcttctaa gtcaattttc tttaaactgg taaccccccg
     4321 gtggggctgc agaagaacag ttctctcgtc taaaagttgc agcatgtcta aatcagaagt
     4381 aaccagcaaa acctgaaagt cagattttac tttttctttt acgctcccca ttaagtcatc
     4441 gccttcccag ccaggcatct ccaaaatgac aaatcccatg agctctaaga gttctttcac
     4501 tattcctatc tggggtttta gttcatcagg catgggtttg cgctgcgctt tataactttc
     4561 ctggagttca tggcggaaag tcttttgagg atggtcgaaa gccaccacca cattgaaagg
     4621 acgataatca tcaaggagct tcaaaaccat attcaaaaaa cccagagtag ctccagtggg
     4681 caaaccagaa gaagtagtta aacggggcag agcataaaaa gcccgataaa ttaaagaact
     4741 tccatctata accactaatt ttttatcttc catttccgga cctcaactga ttgacaaagg
     4801 gggaattttc cttataattt tttagtgaag ccgaagtggc ggaattggca gacgcactag
     4861 actcaaaatc tagcgagctc tgcgctcgtg cgggttcgac tcccgccttc ggcaccactt
     4921 cactctccga ggtaaaaccc ggtgtccgga tgaagcagcg catcttcttc tccttgcggg
     4981 gcgctaactt tcacttccct tccctccacc tgtaattcaa ttttcctcat cccttctact
     5041 tcctcaggag gcaaatctaa ctgttgagca aaaactttct catacagaag agaaagtttc
     5101 tcctgaaaaa cctgatcttc tccaaaccgc tcttcacatt ctctaacaaa ctgataaacc
     5161 agctcctggt cttcatccag attaagaagc aagttatcta aagcacgctc gtatcctctc
     5221 cgttcataag agtaaaagag agagctcaaa ttccttcctc cccatctcat tattttagct
     5281 cctaaataag gagaaaggaa atctttcctc tccaggagaa gaaaagcttg aggcattacc
     5341 tcctgccagg cttcttcttc actgaaggaa ggactgcctc cttctaaaaa tatacttttt
     5401 agtctctgga tatcactttc ccctaaggaa acgtaagagg gagcatagta acgccatttt
     5461 tgaaacacat cccggttatc aggttgagcc attaaaacca cagtccattc ttttagcgct
     5521 tcttctattt tgcctaattg ctcttcagct tttcccagcc aatagcgagc ttcccagaaa
     5581 ttgggatcta tttccagggc tttcctgtac cacttttctg cctcctcata gttaccctga
     5641 gaaaaataaa tatagccatt ctggtaacat tcttctttgg tctgagcaaa aatccccggc
     5701 accactccca gaattagcca ccctatgata accaattgcc cgattttgtt tttcatcctg
     5761 ctaaccagat ttctcccttc tctaaagagt ctttaatcat tctctctact tcccggttcc
     5821 aatcaataca gaatgattct cccagctcca ccgcccagct ttctttctct ccccggatat
     5881 ggagtaccac gggaaaagaa cccggaaaac gacgaagata atcctttaaa aagtaaaccg
     5941 tcttaagggt gtctccttcc tcaggaattg caatgtgtac gatagaggca tttttctttt
     6001 cctctatatc tatcagttct tcagcaatta tcttgtgctc cccattatcc agtaaatcca
     6061 cttttccggt cacccagacc acctgttttc gggaaagata atcttctaac tggagagcta
     6121 actggggaaa aaagaccact tcaatgctac ccgtttcatc ctctaaagta acaaaataca
     6181 tattaccccc attcctggtg gtgatttttt tactcttgtt cactatccct cttattctca
     6241 cttttacttc ctgttttaag tggggaatct gccccagagg aacagcactc caggaagaag
     6301 agaaagattc caccttcaga gggtgatcgc tgatgtagag accgagcatt tctttttcca
     6361 ttctcaacag ttcttcccgg ggtagctctt ctacttcccg gagagaaaag ctctctccat
     6421 tgttattttc ttccccaaag agagattttt gttttttttt gcttttattt ttacgggaaa
     6481 gaagagcaat atcttctaag gaagctaaaa gttgagcccg atgggggtga agctcatcaa
     6541 atgcccctgc ctgcaccagg ctttctaaaa ctcttttatt tatgacccga gaacctactc
     6601 gttctacaaa atcgaaaatg ctcaaaaatt tcccctcttt gcgtcgggtc aaaatttcct
     6661 ccactgcggc accacccaca tttttaatgg ctgccagacc aaacctgatt ttattttctc
     6721 ccactacggt aaaattagct aaactttcat ttatatcagg caacaaaact tgaattccca
     6781 atcttttgca ttccccgata aatttagcca ctttttcatt atcattggcc acactggtaa
     6841 gacaggccgc taaatattcc gcaggatagt gagctttcaa ataagcggtt tgataagaga
     6901 ttaatgcata agcagcacta tgggatttgt tgaatccata accggcaaaa tattcgatga
     6961 ggtcaaatat ctcctctgct ttttccttgc tacatcctcg ctttagcgct ccctctataa
     7021 atttagctct ttgctccttc ataacctcgg gttgcttctt tcccattgcc cggcgcaaaa
     7081 tatctgcttc tcctaaagta aagccagcta attcagcagc aattttcata acctgctctt
     7141 gatacactat taccccgtaa gtttcccgta aaatagcctc cagtgccgga tgaggatatt
     7201 ctattttcac cttaccgtgt ttgcgattaa tgaaatcttc aatcatgccg ctacctaaag
     7261 ggccgggacg gtaaagagct aaaatagcta tcagatcttc gatgttggaa gggttcagcc
     7321 tcttcagtag ttccttcatc ccgctacttt ccagctgaaa aaccccggtg gtctctccct
     7381 tagagaggag agaaaaggtt tcctcatcat gcagaggaat tttctccaag tctatctcct
     7441 ggtgtcgggt atttctaatg atctgcaaag ctttttctat caccgtcaaa gtccgcaacc
     7501 ccagaaagtc catcttcaga agccccagct cctgcagggc atccatctca aactgagtaa
     7561 ctatttcccc ctcccccatc ttctgcagag gaacataatc ggtaagtggc tggttggata
     7621 tcaccacccc ggcagcatgg attgaggcat gacggcacat tccttctatt cgagaagcca
     7681 cctccagaag atgctgcagt tctttgttct gtctggatag ctcccgcagc tgagagttag
     7741 attccagcgc ctttttgagg gtaactcccg gacctccggg aataagttta gcaatcttat
     7801 ccacttctcc atagctccag cccagagctc ttcctacatc tctcactgcc tggcgagcca
     7861 tcattctccc aaaagtgata atctgcgcta cgttggtttt cccgtacttc tcggctacat
     7921 attcgataac ttctccccgt cgctcaaaac aaaaatcgat atcgatatca ggcatactca
     7981 cccgctcggg gttcaaaaaa cgctcaaaga gcaaaccata ctgcaaaggg tcaatgcttg
     8041 taattcccag agtataagat acgatgctac cagcagctga acctctcccc ggccctacca
     8101 ttatgccttt ttgccgggca aaattcacaa agtcccagac aatcaggaaa taggtagaat
     8161 aacccatatc ttcaatcact tttaactcat attctaactg ctctcgggca cgctccgggg
     8221 gagggtctcc atatctgttc actaaaccct gaagacacaa actgcgaagg tatcctggag
     8281 catcaagccc cggaggaaca ggaaattcgg gaagattgat tttccccaat tctaaatcta
     8341 cttgacaccg ctcagcgatt aaagaggtgt tttctaaagc ctgaggacaa taagaaaaat
     8401 ctctttccat ctcctgcggg gacttaaaat aaaattctgc agtgggaaag cgaagcctct
     8461 tggggtcatc cagagtggta gcagtctgga tagctaaaag tacatcatgc actgaagcat
     8521 cttccgcttc taaataatgg cagtcattgg tggccactat tttgatatca tatttattac
     8581 ctatctctaa aagtgcctgg ttaactattc tctgctccgg gattttattt tcctggattt
     8641 ccaggaaaaa attatccttt cccaggattt ctgcatactc cagcgcaact ttctctgcct
     8701 tttctctttc tccgctcaaa attaagctgg gaatttctcc cgcaagacac gccgaaaggc
     8761 cgattaaacc ccgagaatgc tcccgaagga gagctttatc gatacgaggt ttatagtaaa
     8821 aaccttccag gaaagcttta ctcaccagct gcactaaatt cttataccct tcctgatttt
     8881 cagccaaaag aaccaggtga taagcagatt cagccccccg cactacagtt ttttcaaacc
     8941 ggctccccgg cgctacataa acctcgcaac caatgatggg tttaattcct gcttttttag
     9001 cctgagcata aaaatccacc actccataca ttactccgtg gtcggttatg gctactgctt
     9061 ccatcccctg ggattttact ttattcagta aatcatcaat ccggatagcc ccatctaaaa
     9121 gactgtattc actgtggaga tgtaagtggg aaaaactcat tttcactcct cactagaaag
     9181 tactttaatc aaaacattcc tggtcaattc cgggtcggcc tgcccccggg tctctttcat
     9241 tacctgaccg attaagaact gaagggcctt ttctttgcca ctcaagtagt cagctacgct
     9301 ctgaggattt ctctcgatga cgtctctggc tatattctcc agttctttct catcggttat
     9361 aaacgaaatt ccccgtcgag cgatgacttc ccgagctcga gcaccggtag caaacatttc
     9421 ctctaaaaca cttttcgcta tagttccact gatttctttt ttagccacca tcttcagtag
     9481 ctcagctaaa tgtgcagggg taattttcgc ctgagaaatg gtggctccta actctctcaa
     9541 attcttaaaa acttccgtgg ttatccagtt acaaaccatc cgcggttcgt caaaatcccg
     9601 cagggtatct tcaaaaaaat cagctatttc cttatcctgc accaggattt ctgcttctcc
     9661 ctgagatagt tcatattcgg tcataaatct ttcttctctt tctaatggta actccagaag
     9721 ctctgatttt atttcctcaa tcatctcttc tttaatagaa aaaggtaaca aatccgggtc
     9781 ggggaaataa cgataatctt ctgcttcttc tttccctcga gcagaaactg ttatttcttt
     9841 tgcctcatcc cagtggcgag tctcctgaaa aataggtttc ccttcttcta acaatttctt
     9901 ttgcctttcc acttcgtagc tcagtgcccg gtaaacagca cgaaaagaat tcatattttt
     9961 aacttctact ttaactccta aaagcgaaga agaagagaga gaaatattgg catcacagcg
    10021 caacgacccc tcttccatat taccatcgct cactcccaga tagcaaacta tattgcgtaa
    10081 catcatcaag caatcccgag cctcttcagg tgaagaaagg tcgggttcgg taaccatctc
    10141 cgcaagagga attccacagc ggttacaatc cacccctgaa gttccgggag gaagagatac
    10201 tccttcgtga atcaatttac cggcatcttc ttctatgtgt actcttctga ttctcactct
    10261 cttttttact ccctggctgt aaaattccaa aaagccaccc actccgatag gtaaatcata
    10321 ctgggagatt tgatatccct tgggtaaatc aggataaaaa taatttttac ggtgaaacac
    10381 gcactgaggg agaatctggg aatggagagc caaggctgcc cggagtgcta attccaccac
    10441 tttctggttg agaacaggaa gcgaccccgg taaccccagg cacaccgggc aaaccagggt
    10501 gttaggttct tttccaatgt aatcagtact acagtgacaa aacatctttg cttgagtgag
    10561 caactgacag tggacttcta aaccaatggt cgtagtaaaa tcactcattg ttatcctcct
    10621 taaaccctgg aaaaggagaa gggagagcca gaagtttttc taagaaaaga gccaatccca
    10681 gtagtttccc ttcctcaaaa ggtcctccta taatctgtaa ccccacggga agcccttcct
    10741 tatagccaca attcaaggac acagagggaa gtccagccat agctgagggt atggtaaaca
    10801 catcattcaa atacatctcg taggggtctt taacttcctt ccctaaaaag aaaggaaaag
    10861 agggagtagt aggggtaatt aaaagatcac agttttttaa agcttcctca aaatcctgtt
    10921 ttattaaagt cctcaccttc atacctttta aatagtattc atcatagtat ccagaactta
    10981 aacagtaagt ccccagaatg attcttcttt tcacctcttt accaaaacca agagtacgag
    11041 ttttgaaata catatcctgt agattatctc cctctactcg caggccatac atcaccccat
    11101 cataacgagc taaattagag cttgcctccg ccggtgctac tatatagtaa gattccaagg
    11161 cataatcggt atgaggaaga gaaacttcta ctatctcgat tccttctccc cttagcttct
    11221 ctaaagcctc ttgaattttt tctccaattg ctttttctaa tccggaagga aaatactccc
    11281 gggg
//
LOCUS       DAOSVE010000056        11188 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_3284_length_11188_cov_71.453337, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000056 DAOSVE010000000
VERSION     DAOSVE010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 11188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11188
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3284_length_11188_cov_71.453337"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            371..1399
                     /locus_tag="PK016_05775"
     CDS             371..1399
                     /locus_tag="PK016_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXL02611.1"
                     /translation="MTKRQVIKTVLDHEKPPYVPWSFGFTKEAADKLIAYYNSNSHCS
                     IGSKDELEDFVGNHLLKLGSDIGFFEDVGLNLVRDVFGVVWDRSIDKDIGNVQKPVLS
                     KPSLQGYKFPNPLDQRFFQDIPEKIQRYPDRFRVFQIGFSLYERAWTLRGMEQLMMDF
                     YDYPDFVHELLHTIADYNITQVKEAMKYDIDAVYFGDDWGQQRGLQMGPKLWKEFIYP
                     ELRRMYGSVKQEGKYVFIHSCGDVDELFDDLIEIGVDCFNPFQPEVMDIFQLIEKYRS
                     RLSFHGGLSTQQTLPYGTPQDVVRETRRLIDMGRSGGYILAPAHDVEGDVPLPNMLAF
                     IEETQKQI"
     gene            1713..2792
                     /gene="carA"
                     /locus_tag="PK016_05780"
     CDS             1713..2792
                     /gene="carA"
                     /locus_tag="PK016_05780"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HXL02612.1"
                     /translation="MKALLALEDGKIFQGYAFGVEGEAEGEVVFDTGMIGYQELLTDP
                     SFRGQIVTITYPLVGNYGLNSQDSESSRPQVESLIVREKSTMYSNWRGEETLEEYFKR
                     HNLLGLERIDTRALTKHIREKGALRGVISTRDLDPASLVKKAKKVPSIVGQDLVKKVT
                     ASAPYVWQEKGKFNILVVDCGVKFSILRELAKRDCRVVVYPATSSAEEIIREKPQGVV
                     ISNGPGDPAALSYLVELVRKIIGKFPILGICIGHQIMAQAVGGKTSKLKFGHHGTNHP
                     VKDLRSGWVAMTTQNHSFVVAPDTLPPEIEITHINLNDFTLEGMKHKYLPFFSVQFHP
                     EAHPGPHDTTYLFDEFIKMVEDNLG"
     gene            2785..6054
                     /gene="carB"
                     /locus_tag="PK016_05785"
     CDS             2785..6054
                     /gene="carB"
                     /locus_tag="PK016_05785"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HXL02613.1"
                     /translation="MAKREDIQKILIIGSGPIIIGQACEFDYSGVQGCKALKSEGYEV
                     VLVNSNPATIMTDPEMADRTYVEPLVPEVLEKIIERERPDALLPTLGGQTALNLTVQL
                     EERGVLDKWGVKVLGASPQAIKKAEDRRLFKESMTKIGIEVPRSGQAYSLKEAIEVAK
                     EIGFPLIIRPSFTLGGTGGAIAYNIEEFEDLAQKALDSSMIREILIEESVMGWKEFEL
                     EVMRDAKDNVVVICSIENFDPMGIHTGDSITVAPAQTLTDREYELMRDLAIKAIREIG
                     VDTGGCNIQFAVNPQNGRMVIIEINPRVSRSSALASKATGFPIAKIAAKLAIGYTLDE
                     IPNDITRKTPASFEPAIDYCVVKIPRFTFEKFPETEPWLGISMKSVGETMAIGRNFKE
                     ALQKGLRSLEIGKCGLEDVKNWESLSKVKRQEILRERLAKPNAERVFYMKMAIKEGLS
                     TEEIYDLTKIDPWFIHEISEIVEMEEGLKGVRDWKNLEPELIKRAKEFGFSDRQLAGF
                     WNTKEEEIRKWRKEKGIEAVYKQVDTCAAEFPAETPYYYSTYEEECEANPSNREKVVI
                     LGAGPNRIGQGIEFDYCCVHATWGLRDLGYETIMVNSNPETVSTDYDTSDKLFFEPVY
                     LEDVLNVVEKENPQGVILQLGGQTPLNLAKDLERAGVKILGTSPESIDIAEDRDRFKE
                     LVDKIGLKQPRSGIATSFEEAHLVASQIGYPVMVRPSYVLGGRAMKIIYSEEELKDFV
                     SQAVEVSAGHPILIDKFLEDAIELDVDAISDGEITIIAGIMEHIEEAGIHSGDSACVI
                     PPFSLSDEIVEKVKQITYTLARELKVIGLMNVQYAAKDDEIFVLEVNPRASRTIPFVS
                     KATGVPYARLASQVMAGRKLREIGLTQEVKIDYFAVKEVVFPFRRFSGVDPVLGPEMR
                     STGEVMGIDSDFGMAFAKSQIAAQSRLPLEGTVFISVKNRDKRGIIFIAKKLRDLGFK
                     IVATRGTAKVLSNNGIPVEVVAKVGEGRPNVVDVIKNREIDLIINTPSGGRAQLESSI
                     IRKEAIIRDIPFVTTLSGAEATVYAIERLKSGSVAVKSIQEYHQEIFERQGITLTMRK
                     D"
     gene            6054..6884
                     /locus_tag="PK016_05790"
     CDS             6054..6884
                     /locus_tag="PK016_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HXL02614.1"
                     /translation="MLEADVSLLKTILIRALNIAIIIGVSYFILSISERLIRRLLTWG
                     DKYTGRREVARRLNTLVPLLRSLLKYVIIFFAAILILRQLGVDATAILAGAGIVGVAV
                     GFGAQTLVKDILTGVFLLLEDSISVGDTVQIGDISGTVEDIGLRVTRIRPFSGALIII
                     PNGEISRIANYNRGFTRAIVEIRIAYEADIDRATSILEEIAKKYHEEHPEKILEPAAV
                     HRIVRLDSSGVVLRLVLKVAPNKHWDVERDMLYLIKKTFDQENIEIPYQKHIVYIKEP
                     "
     gene            7255..8943
                     /locus_tag="PK016_05795"
     CDS             7255..8943
                     /locus_tag="PK016_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HXL02615.1"
                     /translation="MDLNLLVGGEAGQGVQTVSGALSTILQRSGYFVFSIEDYQSRIR
                     GGHSFTQVRFSNIPCRAALKMVDILICLNEETYELHRERVKEGGWILGAVKKEEGAPS
                     FHLIPLNFEEIAKEVGNKIFANVVAIGAILGILGLDFKIADNYLEENFARKGKEIVEK
                     NKQALKRGRDEVIPYFFSSLIEGTKREKIPLLVLDGNQALGLGALLSGCQFYSAYPMT
                     PSTGILNFLSSRAQDYGIAVEQAEDEISAINMAIGASYAGARAMTGTSGGGFCLMSEG
                     LGLAAMTETPLVVVDAQRPGPSTGLPTRTSQGDLEFVLHASHDEFPRFVFAPREPQEA
                     INLVIRAFNLAEKYQVPVIILSDQYLADSKWTYENLEVRERPDVPQFSSFPLPYRRYL
                     ISPSGVSPRAIPGLSKALVVADSDEHDEFGHLTEDLSLRVEMHQKRMRKKETMKEEVR
                     LPYYEEGKDLTLVGWGSTWGVIKEVQERLRMEGIDSGIIHFSEIYPLPLKIEELFGHT
                     SRPIVIENNYGGQLANLLEGELHLPFPLRINSYDGRPFLVEELLLKVKEVGKNE"
     gene            8936..9790
                     /locus_tag="PK016_05800"
     CDS             8936..9790
                     /locus_tag="PK016_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXL02616.1"
                     /translation="MSNSFDSQIEIAWCPGCGNFGILRALKGALTELEFSPEEVVLVS
                     GIGQAAKLPHYIAVNVFNGLHGRSIPVALAIKTVNPRLLVIAESGDGCMYGEGGNHFL
                     HALRRNPDITVIVHDNQVYGLTKGQASPTSEEGFVSKAQPWGNISTSLNPLSLAISQD
                     ASFVARGFSGDVDRLKEIIKAALTWNGFSLVDVLQPCVTFNRVNTFAWYKERVYYLED
                     SYQPEDRIRALEKSLEWGDKIPLGVIYRHQRPTFQDKIYSRIEKEDLIYRGIDFDDLL
                     SRELKLFV"
     gene            complement(9958..10407)
                     /locus_tag="PK016_05805"
     CDS             complement(9958..10407)
                     /locus_tag="PK016_05805"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXL02617.1"
                     /translation="MEISHILVPTDFSPFSDLALENAASLAEKFGAKITILHVLTLAE
                     VNALTSQPGNPWENVMLNIQNDTREEFQKNAGNYSLVEEPALEVAVGEPAEEIAKFAE
                     ENGVNLIVMGTHGRTGLASIFLGSVTIGVIKKTCLPVMVVKCAESLK"
BASE COUNT         3308 a         1912 c         2606 g         3362 t
ORIGIN      
        1 tttatctcca gcattatagt tttaatcttt cttatctaat ttgcttttta gtattgtgcc
       61 gtaggctgtc tgtcttttcg gggtttataa gggggcaaag ctttcttata gccctcaccc
      121 taaccctctc ccttcgaggg cgagggaaaa aagatgtaaa gctctccctt cgagggcgag
      181 ggaaggaata tgtggggttc ttccattgaa ggcaagggaa gaaaaagaaa gaggattata
      241 aagggggtct cccctttata gtatttgtaa tggattttgc ggttaactat aattaagcac
      301 tagaagttat tatatgtcag ctcgagttta ataaagcatg aagaacccgg ttttggaaag
      361 ggggtacatt atgacaaagc gtcaagtaat aaagacggta cttgaccacg aaaaaccgcc
      421 ctatgttccc tggtcttttg gttttactaa agaagcagct gataagctca ttgcctatta
      481 taatagtaat tcccattgct caattggttc taaggatgag ttggaagatt ttgttggtaa
      541 tcatcttttg aaattaggga gcgatatcgg ttttttcgaa gacgtaggac taaacttggt
      601 acgagatgtt tttggagtag tttgggaccg aagcatagat aaagatattg gtaatgtcca
      661 aaaaccagtg ttgtctaaac caagcttgca gggatacaag tttcctaatc ctctagacca
      721 acgttttttc caggatatcc cggaaaaaat tcagcgctat cccgatagat ttcgggtatt
      781 tcagatcggt ttttcgttat atgaacgagc ctggaccttg aggggtatgg aacaacttat
      841 gatggatttc tatgactatc ctgattttgt gcatgagctc ttgcacacta tagctgacta
      901 taacattact caggtaaaag aggctatgaa gtatgacata gatgctgtat acttcggcga
      961 tgattgggga cagcaacggg ggttgcaaat ggggccaaag ttgtggaagg agtttatcta
     1021 tccggaatta cgtcgaatgt atggaagtgt taaacaagaa gggaagtatg tatttattca
     1081 ttcgtgcggt gatgtagatg aattattcga tgatttgata gaaatagggg tggactgctt
     1141 taatcctttt cagccagaag ttatggatat cttccaatta atagaaaaat accggagtcg
     1201 cttgagtttt cacggcgggc tttctactca gcaaactctt ccctacggca ctccccaaga
     1261 tgtagtccgg gaaacaaggc gtcttataga tatgggacgt tcaggaggat atatccttgc
     1321 tcccgctcat gatgtagaag gagatgttcc tcttcccaac atgctggcct ttatcgaaga
     1381 gactcagaag caaatataat ctcatcaatt ttatttagtt caaatagttt tcatctttct
     1441 tcccccaacc tctcctgtca ggggcgagag aaaaaaagag aaattcaagt agcaaagtaa
     1501 taaaaagaaa acccgccatg atagcgagat gtagcttctc cagcgtaatt cagtttttgt
     1561 tcctggtttt catcttgcat tgtagatttc tctgtatatt cctcgcaata gccaaatgag
     1621 gctcagacct tgtttaacgg gctttgtgtg atgttacaac cttagaaaat taccccttga
     1681 caattgtgtt atattagacc gagggtgttg gaatgaaagc gttattagct ttggaagatg
     1741 gaaaaatttt ccagggttat gcttttggcg tagagggaga agcagaaggg gaagtagttt
     1801 tcgataccgg catgatcggt taccaggagc ttctcactga cccttctttt cggggccaaa
     1861 tcgtaaccat aacttatcct ctcgttggta attacggtct caactctcaa gattcagaat
     1921 cttcccgtcc tcaagtagaa tctctaattg ttagagaaaa aagcactatg tatagtaact
     1981 ggagaggaga agaaacctta gaggaatatt ttaaacgcca taatttgcta ggattggaaa
     2041 gaatagacac tcgagctctt accaagcata tccgggagaa gggagccttg cggggggtaa
     2101 tctccacccg ggatttagat cccgcctctt tagtaaaaaa agctaaaaaa gttccatcaa
     2161 tagtgggcca ggatttggtt aaaaaagtta ctgcttctgc accttatgtg tggcaagaga
     2221 aaggaaaatt taacattctg gttgtggatt gtggagtgaa gtttagcata ttaagagaac
     2281 tagctaaaag agattgtcga gtggtagttt atcctgctac ttcctcagcg gaagaaatta
     2341 taagagaaaa acctcaagga gtggtcatct ctaatggtcc gggagacccc gctgctcttt
     2401 cttaccttgt agaattggta agaaaaataa tagggaaatt tcctattctg gggatatgca
     2461 ttggtcatca gataatggct caagcagtgg gagggaaaac ttctaaacta aagtttggcc
     2521 atcatggaac taaccatccg gttaaagatt tacgctccgg ctgggtagcc atgaccactc
     2581 aaaatcatag ttttgttgtt gcccccgata ctttacctcc agagatagaa attacccata
     2641 ttaatctcaa tgattttacc ctggagggaa tgaaacataa atatctaccc tttttctcgg
     2701 tgcagtttca tcctgaggct caccctggtc ctcacgatac tacctatctt tttgatgagt
     2761 ttataaaaat ggtggaggat aatcttggct aaaagagaag atatccagaa aattctgatt
     2821 attggttctg gtcctataat aattggccaa gcctgtgagt ttgactattc aggagtccag
     2881 gggtgtaaag cgctaaaaag tgaaggttat gaagtagttt tggtgaatag caatcccgct
     2941 accattatga ccgatccgga aatggctgac cgtacctacg ttgagccttt agttcctgaa
     3001 gtcttagaaa agattataga acgggaacga cctgatgctt tgcttcctac gttaggagga
     3061 cagactgccc taaacttaac cgtgcaactg gaagagcgag gagtattaga taagtgggga
     3121 gtaaaagttt taggtgcctc tcctcaggct attaaaaaag cagaagaccg ccggcttttt
     3181 aaagaatcta tgactaaaat agggatagaa gttcctcgga gtggtcaagc atacagttta
     3241 aaagaagcaa tagaagtggc taaagaaata gggtttcctc tgataataag gcctagtttc
     3301 accctgggag gaacaggggg agctattgct tataatattg aggaatttga agatttagct
     3361 caaaaagccc tggatagcag tatgattcga gaaatattga ttgaagaatc agtaatggga
     3421 tggaaagaat ttgagctgga agtaatgaga gatgctaaag ataatgtagt ggttatctgt
     3481 tccattgaaa atttcgaccc tatgggaata catactggtg atagtataac tgtggctcct
     3541 gctcaaacat taactgaccg ggaatatgag ttgatgcgag atttagctat caaagccata
     3601 agagagattg gagttgatac cgggggttgt aacattcaat ttgccgttaa tccccaaaat
     3661 ggtcgaatgg taattatcga aattaatccc cgggtttctc gttcctcagc tctggcttct
     3721 aaagctacgg gtttccctat tgctaagatt gctgctaagc tagctatagg ttatactctg
     3781 gatgagattc ccaacgacat tactcgtaaa actccggctt cttttgagcc ggctatagat
     3841 tattgtgtgg tgaaaatccc ccgttttact tttgagaaat ttcctgagac tgaaccatgg
     3901 ttgggaattt ctatgaagag tgtaggagaa actatggcca ttgggcgtaa ttttaaagag
     3961 gcccttcaaa aagggcttcg ctctctggag attggaaagt gtggcttaga ggatgttaag
     4021 aattgggaaa gtttatccaa agtaaagaga caagagatat tacgggaaag attagctaaa
     4081 cccaatgctg agagagtctt ttatatgaag atggctatta aagaaggttt aagcactgaa
     4141 gaaatttatg atttaactaa aattgacccc tggtttatcc acgagataag tgaaatagtg
     4201 gagatggaag agggacttaa aggggtaagg gattggaaaa atttagaacc agagttgatt
     4261 aaaagagcca aagaatttgg tttctctgac cggcagttag ccggattttg gaatactaaa
     4321 gaggaagaga taaggaagtg gcgaaaagaa aaaggaatag aggcagttta taaacaagta
     4381 gatacctgtg ctgctgaatt tcctgccgaa actccttatt attattctac ttatgaagaa
     4441 gaatgtgagg ctaacccctc aaatagagaa aaagtggtta ttttgggagc agggcccaat
     4501 cggatagggc aggggattga atttgattat tgttgtgttc atgctacctg gggtttgaga
     4561 gacttaggtt atgaaactat aatggtaaat agtaatccgg aaacggtaag tactgattat
     4621 gacacttcag acaaattgtt tttcgagcca gtatatttgg aagatgttct taatgtggta
     4681 gaaaaagaaa atcctcaggg ggtaatactg cagctgggag ggcaaactcc tcttaatttg
     4741 gccaaagacc tggaaagagc aggagttaaa attttaggaa cttctccaga aagtattgat
     4801 atagctgagg accgggaccg atttaaggag ttggtagata aaattggact aaaacagccc
     4861 cgtagtggaa tagctacctc ctttgaagaa gctcatttag tcgcttctca aattggttac
     4921 ccggttatgg tgcgtccctc ctatgtttta ggaggaaggg caatgaaaat aatttatagt
     4981 gaagaagaat tgaaagactt tgtctctcag gcagtagaag tatctgccgg gcatcctatt
     5041 ttaattgata agtttttaga agatgcgata gaattagatg ttgatgccat cagtgatggg
     5101 gagattacca ttattgccgg tataatggaa catattgaag aagcaggtat tcactctgga
     5161 gatagcgcct gtgttatacc tcctttttct ctaagtgatg aaattgtcga aaaagtaaaa
     5221 cagattactt atactttagc tcgagagcta aaagtgatag ggcttatgaa cgttcagtat
     5281 gcggctaaag atgacgaaat ttttgtttta gaagtaaatc ctcgtgcttc tcgtactatt
     5341 ccttttgtga gtaaggctac gggggtacct tatgctcgtc tggcctctca agtaatggcg
     5401 gggaggaaat tacgggaaat tggcttaact caagaagtca agatagatta ctttgcagtt
     5461 aaggaagtag tttttccctt ccggcgtttt tctggggtag acccggtatt aggcccggaa
     5521 atgcgctcta ccggggaagt gatggggata gatagcgatt ttggcatggc ctttgctaaa
     5581 tcccagattg ctgctcaatc tcgcctacct ttggagggga cagtattcat tagtgttaag
     5641 aaccgagata agagaggaat aatttttata gctaaaaaac ttcgagattt aggtttcaag
     5701 atcgtggcca cccgaggaac agctaaagtc ctgagtaata atggtatccc ggtagaggtg
     5761 gtagcaaaag taggcgaagg acgtcccaat gtggtagatg tgattaaaaa ccgggaaata
     5821 gacctgatca ttaacacccc ttcgggagga cgagctcagt tagagagtag tattattcgc
     5881 aaagaagcaa ttattcgcga tattcctttt gttaccactc tctcaggagc agaagctacg
     5941 gtttacgcta tagagcgtct caaatctggc tctgtagctg ttaaatctat tcaggaatat
     6001 catcaagaaa tttttgagag acagggtatt actcttacta tgaggaaaga ttgatgcttg
     6061 aagccgacgt tagtcttttg aagacaatac ttataagagc cttaaatatt gctattatta
     6121 taggtgtctc ttattttatt ctgtctataa gcgagagatt gataagaagg ctcttgacct
     6181 ggggagacaa atatactggt cggcgagagg ttgcccgccg actcaatact ttagttcctt
     6241 tactgcgaag tctgttgaaa tatgtgatta ttttttttgc tgctatttta attcttaggc
     6301 agttaggagt tgatgctacg gcaattctgg ctggagcggg aattgtaggt gttgccgtgg
     6361 gttttggtgc tcaaactcta gtcaaggata ttttaaccgg tgtttttctc cttctggaag
     6421 acagtatttc agtaggagat acagtacaaa ttggtgacat tagcggtacg gtagaagata
     6481 tagggttgcg ggtgacccgc attcgtcctt tttctggagc tcttattatt atccctaatg
     6541 gagagatttc tcgtattgcc aattataatc gaggttttac ccgggctatt gtagagataa
     6601 ggatagcata tgaagcagat atagaccggg ctacaagtat tttagaagaa atagcaaaaa
     6661 aatatcatga ggaacacccg gaaaagattt tagagccagc tgctgtccat cgaatagttc
     6721 gtttagattc ttccggtgta gttttaagat tggtactcaa ggtggctccc aataaacatt
     6781 gggatgtaga aagggacatg ctctatttaa taaagaaaac ttttgaccag gagaacatag
     6841 aaattcctta tcaaaaacat attgtatata taaaggagcc ttaagttcta tgttatcttg
     6901 caaaacctct aaaattcggc aataacaggt ctcttttatt tacctcttac acttctcttt
     6961 tgacttttct ttccccttct tcgaagtcat ttcttctctt ctggagagaa gaatccgtat
     7021 ttttcctcgt ccttcctgtg aaaactctac atattctttt tattaccttc tcgctccctt
     7081 cagggggaaa ataaaaggga aatactgcaa gagatggaaa gaaggagata taagggttgg
     7141 gatgagtatt atcctaaatt tctcagtaat atggtaatat aaatataccg agatattaaa
     7201 ggaaactgta gaagcaagaa gcaaatttta tttcctaaag gaggtaaggg ggatttggac
     7261 ttaaatttat tagtgggggg agaagcggga caaggagtac aaactgtttc tggagcttta
     7321 tccactattt tacaaagaag cggatatttc gttttttcta ttgaagatta tcaatctcgg
     7381 ataagaggag gacatagctt tacccaggtt cgcttttcta acatcccctg tcgagcagca
     7441 ttaaaaatgg tggatatcct gatctgcctt aatgaggaaa cttacgagct gcatcgggaa
     7501 agggtaaaag aggggggatg gattttaggc gcggtaaaaa aggaagaagg tgctccttct
     7561 tttcatctca tacctttaaa ttttgaagaa attgctaaag aggtagggaa caagattttt
     7621 gctaatgttg ttgctatagg tgctatatta ggtattttag gattggattt taaaattgct
     7681 gataattatt tagaggaaaa ttttgcccgg aaagggaaag aaatagtaga gaagaataag
     7741 caagctttaa aacgaggcag agatgaagta attccctatt ttttttcctc gctaatagag
     7801 ggaactaaaa gagaaaaaat tcctctttta gtcttggatg gaaatcaggc tttaggctta
     7861 ggggctttat taagcggctg ccagttttat agtgcttatc ctatgactcc ttctacggga
     7921 attttgaact ttctttcctc tcgggcccag gattatggaa tagcagtgga gcaagcagaa
     7981 gatgaaattt cggctattaa tatggctatt ggtgcttcct atgctggagc tcgagctatg
     8041 acgggaacat caggaggtgg tttttgtctg atgagtgaag gtttggggct ggcggctatg
     8101 acggaaactc ccttagtggt agtagatgct cagagacctg gtcctagcac tggtttgccc
     8161 actcgaactt cgcaaggaga tttagagttt gtgctccacg cttcccacga tgaattccct
     8221 cggtttgttt ttgcacctcg tgagccccaa gaggctatta acttggtaat tagggctttt
     8281 aatttggcag aaaaatacca agtgccagtc attattttaa gtgatcaata tctggctgat
     8341 tccaaatgga cttatgagaa tctagaagta agagaaagac cagatgttcc tcaattttct
     8401 tcgtttcctc ttccttatcg acgttacctt atatctcctt caggtgtttc tcctcgagcc
     8461 attccgggat taagtaaggc attggtagtg gcagacagcg atgagcatga tgaatttggg
     8521 catctcaccg aagatttaag cttgagggtg gaaatgcacc agaagaggat gagaaaaaaa
     8581 gaaacaatga aagaagaagt tcgcctccct tattacgaag aaggaaaaga tttaacttta
     8641 gttggttggg gttctacttg gggagtgata aaggaagtac aagagagact tagaatggag
     8701 ggaatagata gtggcataat tcacttcagt gaaatttatc ctcttcctct aaaaatagaa
     8761 gagcttttcg gtcatacttc tcgtccaata gtgatagaga ataattatgg agggcaatta
     8821 gctaatcttt tagaaggaga gcttcatttg ccttttcctc tcagaatcaa tagctacgat
     8881 gggaggcctt ttttggtgga agaattgctt ctaaaagtta aggaggtagg gaaaaatgag
     8941 taactccttt gatagtcaga tagaaattgc ctggtgtcca ggttgtggga attttgggat
     9001 tttaagagct ctaaaaggag cgctcacaga gctagagttt tccccggaag aggtagtttt
     9061 ggtttctgga atcggtcagg cagctaagtt acctcattat atagcggtaa atgtgtttaa
     9121 tgggttgcac gggagaagca ttcctgtggc tttggcgata aaaacggtga acccccggct
     9181 tctcgtaata gcagaatcag gagatggatg tatgtatgga gaagggggga atcattttct
     9241 ccatgctctg cgacgaaatc cggatattac agtaattgtg catgataacc aggtttacgg
     9301 tctaactaag gggcaggctt ctcctacttc agaagaggga tttgtttcta aagctcagcc
     9361 ctggggaaat atatctactt ctttaaaccc tttgagcctg gctatttctc aggatgccag
     9421 ttttgtagcc cgggggtttt ctggggatgt agacaggttg aaagaaatta taaaagcagc
     9481 tctcacctgg aacggttttt ccctggtgga tgttttgcaa ccgtgtgtga cttttaatcg
     9541 ggttaatacc tttgcttggt ataaagagag agtttattat ttagaagata gctatcagcc
     9601 ggaagataga attagagctc tggaaaagag cttagagtgg ggagataaaa tccccttagg
     9661 ggtgatatat cggcaccaac gaccaacttt ccaggataaa atatactccc ggattgaaaa
     9721 agaagacctc atttaccggg gtatagattt cgatgattta ctctctagag aattaaagct
     9781 gtttgtttga gctatttggt taagcttttt gctgtaagta tagctcccga ctttgcagtt
     9841 tctactgatt tttattcatt tcgaagtcac aaacccggta aatatagaca tctgtttttt
     9901 ctaaaaatgg agtattaagt tcgggaagtc agatacatct gacttcccga acttagatta
     9961 tttcaaactc tcagcgcatt ttactaccat aaccggtaag caggttttct ttattactcc
    10021 gatagtgacg ctacctaaaa atatgcttgc taaaccagtt cttccatggg ttcccataac
    10081 tataagattt actccatttt cttcagcgaa tttggctatt tcttctgctg gttctcccac
    10141 tgctacttct aatgctggtt cttcaacgag agagtaattt ccggcatttt tttgaaactc
    10201 ttctctcgta tcattctgga tgttgagcat aacgttctcc caggggttgc caggctgaga
    10261 agtgagggca ttaacctcag ccagagttag aacgtggaga atggttatct ttgctccaaa
    10321 tttttctgct aaagaagcag cattttccag agctaagtcg gaaaagggag aaaaatcagt
    10381 aggcaccaaa atatgggaaa tttccatatt tttcctcctt tggtaacttt tctttaagct
    10441 taacctattt ttgctaaaaa gagaagatgg gttctatgtt ttctaatgat atttgttcaa
    10501 aaatttttag aaggatacta tcatgcaata acaacccatg aagttcatat ttcttaacaa
    10561 gatttttaat ttttactaca aaattatcat gtggtctgat tgcaaatagc gttttaatag
    10621 agccagcaat atggagtatt gagagccagc agcgcggaga aagaggacca ccgtaaggta
    10681 gccctctttt cttaggataa tttggaccgc atggaatttt tcccttgaat cacgatgtga
    10741 taagactggg catcgatacg atctgtgatt gcctcagcag ctaccggaat aggaatctga
    10801 tccaaccatt cagctggttc aaattgggaa actacgatag tcgagcaggt tgtcgctctg
    10861 cgatcaataa ttgaaagcaa catttgagtt tcttcctctg tgataggaaa gagaagccat
    10921 tcatctataa tatagtatcc cgcaaaactg attaaaatat taaaactttt aaaaagtaaa
    10981 ttatatcgaa agtcaaaaac tcaaggttgt tatgctggag tattttatct ccagcattat
    11041 agttttaatc ttttttatct aatttgcttt taagtattac gccgtaggct gtctgtcttt
    11101 tggggggtta taagggggta aagctttctt ataaccttca ccctcaccct aaccctctcc
    11161 cttcgagggc gagggaagga atatgtgg
//
LOCUS       DAOSVE010000057        11111 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_3310_length_11111_cov_78.581042, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000057 DAOSVE010000000
VERSION     DAOSVE010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 11111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11111
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3310_length_11111_cov_78.581042"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            426..1400
                     /locus_tag="PK016_05810"
     CDS             426..1400
                     /locus_tag="PK016_05810"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXL02618.1"
                     /translation="MRKFLMLALVCVLFLSVSGVVWAQAWPEKEVEIIIPWEAGGATD
                     VLYRAVAKYFPKYANGKQLIIKNVPGGGSAIGYGEGAKARPDGYTLVAAVNPVITRAL
                     MGPAPYHPIESFDPVCLLVNNPCYILVNSKLKKWNSLSELVEYVRENPGVVTVGNSGA
                     GGGNHLVALSFENAFDLKFIHVPFDGGGPSVNALLGGHIDTVVASSPEGFPNVEAGEL
                     EMLAVFAEERLEKFPQFPTAQEQGYDFTAFMWRGVVVPKGVDPEIVEQIAEVFKAVLE
                     DPDFQKDAENLGQNALYLGPEEFGKHLVSEFERYQEIITKYGLGEFYK"
     gene            1458..1934
                     /locus_tag="PK016_05815"
     CDS             1458..1934
                     /locus_tag="PK016_05815"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXL02619.1"
                     /translation="MGKADIIIGFSLLFVSGFLLIEAMGLPQSHVGISPGLFPAIALG
                     GLMVLNLFLILRGLKQRREKAQSYSASLNLNFEKIKKIAIFVAFAWLYIFLLPLLGFV
                     YASGIFFWAIFILAGVNQWGKALILSFGSSIIMYFVFYRIFMVALPRPALPIPYPF"
     gene            1959..3431
                     /locus_tag="PK016_05820"
     CDS             1959..3431
                     /locus_tag="PK016_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXL02620.1"
                     /translation="MDVIQISLLEGLRQILDFQTLLYIFGGVVFGFIVGALPGLSAST
                     GIVLVLPLVYHIPAAEAVMIMLGIYGGTMFAGSIPAILIRTPGTPSAAATVLDGYPMA
                     QRGEAGLALSTSAIASFVGSLLSAVALFLIAPQIARVALKFGPPEFFSLVVFGLTVIA
                     SVTGKDVIKGLISALLGLFLGTMGMDPISGYSRFTFGSYALEGGLPLLPVMIGLFAVS
                     QVLRDIEEAGVTYQVINKIGRIWPSWEELKRILGASLFGSTLGTLIGAIPGIGGSIAT
                     FLAYGQYRKFSKRGEEFGSGVLEGVAVPEAANNATTGGALIPLLTLGVPGDIVTAVML
                     GILILFGVNPGPMLFREQPEVVNRLFASMFVIAFFILVLGLFGARFSPKILAIPKKIL
                     SPLVLLLCIVGAYADSSSTFGITVALVFGVMGYFMEKFDFPVAPLLISMILGPIAEGE
                     LGRSLLISRGDWSILFVRPISLCFLILAGLSLFFAFRKKD"
     gene            3447..4574
                     /locus_tag="PK016_05825"
     CDS             3447..4574
                     /locus_tag="PK016_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HXL02621.1"
                     /translation="MLEKNNLDSSVKQEIQEKLVRLRKLIEGKNLEALLLKRHVNFSW
                     LTAGGTNVLCMAQDTGVSSILVTDDRSYVIVSNIEAPRMKEEEKVVAKGFELVEYPWY
                     EGNEMKIVREICGEGSIGCDIAFPGTQDLSGEINRCRYQLLETEKERYLWLGKEVSWT
                     LEEVLCSLHPGVLESEVAAEIALRLWKKRIEPIGLQAAADERAYWFRHPVAFRNPAKK
                     LLLASVMARYGGLVTTATRMVYFGTPSPEFLKQYHDNVRIECEMIALSRPGNKAVLPL
                     QRAVELYKELGYPEEWKHHHQGGAMGYLPRDYRVDFECQENILENQAFCWNPSIAGTK
                     SEDGFISTSHGPLFITYPVRFPVFSLEVGDFHFERPDLLVL"
     gene            4587..5621
                     /locus_tag="PK016_05830"
     CDS             4587..5621
                     /locus_tag="PK016_05830"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXL02622.1"
                     /translation="MGHKRIGFAIVGYGLAAKLHAQALTQLDEAKLIAIVGRNIEKAS
                     ELAKNFGVEALSLDEALKREDIQAFIVTTPTGSHLEVVREIAPRGKHLLVEKPLESNL
                     RRTDELIRTCEEAGVILGAVFQHRYDDASLMLKEYKDKGLLGRAAMGSAYVKWYRSQE
                     YYDARSWRGRVETSGGGALAIQASHSIDLLLWFLGEPEKVCGFMNTLAHTGIEVEDAA
                     VVSIQFKNGALGSIEASTATYPGFPERLELHFERGSAIIEGGKIVYLNTLSEKIPVIE
                     RGQSGLSGASDPAGVDIQPFVRMLQDFVNALREGKNPPLDGREGRKAVELIEAVRISA
                     LESRVVELPL"
     gene            5759..6739
                     /locus_tag="PK016_05835"
     CDS             5759..6739
                     /locus_tag="PK016_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXL02623.1"
                     /translation="MTEGCLKGVFVLVVLLVLVLGMTSLAFGEVILKVGSPFEEGHIL
                     VEAATRFKELLEERSEERLQVELQIAQASEEEVNTQCSEGVIDLQFNGGRPVEVFAPQ
                     YFFFNAPYVIKDYDHFLRVWNGPLGEKAKELVLQNGNMFCLGTVYRGFRQMTSLNPIR
                     TLEDLVGLKLRLPVIKTWIAVWESLGVEPVPVPLPELYQALKERKAEASEGDLPQIYS
                     FKLYEVQKYLGITNHLVGVGWIAMNRNSMEQLSPEDQDLVRGCVEEACQWATEKIKTG
                     ESDLLAELQELGMEIVYPDADAIREKAKPAIEELFVTEWPVTTWEEVLAQ"
     gene            7121..8071
                     /locus_tag="PK016_05840"
     CDS             7121..8071
                     /locus_tag="PK016_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014511270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HXL02624.1"
                     /translation="MLVGFWASVALAQEQYVLRFNHVLAPGHPYHEGLQKWAERVYER
                     TNGGLKIEVFHSAQLGVEEDIIEQIRQGVPVGQNTDAARLGNYVPEIAVINGPYFVES
                     IEEVQKLHESPSIQAWIDKLDKEYGLKVLSFNWVQGYRHFMTKDKQVRTPEDLKGLRI
                     RTAPAPIWQESVRALGATPTALNFDEIYTALQQGAIDGAELVYDNVTSGNLYEVLKYI
                     SETRHFLLVNFEVVSSKWFYSLPEEYQKILVEECDRAGLETSYLIEENTEKNKEIIRE
                     KGVTIIEDVDLDAFRKAGEAAYEVLGISEAKNKVYQEIGK"
     gene            8189..8716
                     /locus_tag="PK016_05845"
     CDS             8189..8716
                     /locus_tag="PK016_05845"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HXL02625.1"
                     /translation="MTIKKIYDLIGRGEEVAAGILMVAIMVVVFAAGFGRTIGYPIPW
                     GMEMSTFLFAWAVFFSADAAWRKNRHVSVDILVNKFPEKVRYYIALVNHLLIFGFLVL
                     LIGYGIPLCYHTRFRAFQGIPGFSYMWATLSVPVGSALILITTSLRIRELIKNGKITI
                     SEVESEEGMDLDVED"
     gene            8746..10026
                     /locus_tag="PK016_05850"
     CDS             8746..10026
                     /locus_tag="PK016_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HXL02626.1"
                     /translation="MLLVSIVFIVLLLLGMPVAFAIGISGFLFFVQQPELPLTMPVQL
                     ILSGTQSFSLLAIPMFIFAGNLLNNTGITSRLVKFASVVTGHMRGGLAQTSVVLSTLM
                     GGVTGSSIGDASMEARILGPAMTQRGYDRAYSAAVHGFTSLITIAIPPGIGLVLYGSI
                     GEVSVGRLFAGGILPGLLMAALFMVVVSVTSGKRGYLPEREKRAPFKEIGLSFINSLW
                     AIFFPIFLLVALRFGFLIPSEAGAFASVYALAVGLIAYRELTWKKFVSAIKYTLMDVG
                     MVMFLIALSSLISYGMTWEMIPQTISQFLLGISENPYIITGLIVLLLLVLGMFIDSTV
                     LILLLTSILVPVMSEIGMDLVHFGVLMVITCATGLLTPPVGLTMYAVCSIMECSLEDY
                     MREAWPFLIAMFILIALILFFPQIVLFIPNLIFG"
     gene            10060..10800
                     /locus_tag="PK016_05855"
     CDS             10060..10800
                     /locus_tag="PK016_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXL02627.1"
                     /translation="MEGKVAIVTGGVAGIGRAIADRLAERGYTVIVCDINREKGEVLE
                     KANHKVKGAYVFKYCNVGEEEDIKRVVEEVERDYGGISVLVNNAGIIRRRKEGEIKIS
                     DWDDVFKINTRGTFIFCKYVSEVMKKQKSGRIINISSVASKMGDITSAPGYGPSKAAV
                     NGLTKTFARELAPYNITVNAVAPHAIETEMSAEWSEEMRQKIISEIPLKRLGKPFEVA
                     AVVEFLISDEAGFITGEIIDVNGGFLMD"
     gene            10828..>11111
                     /locus_tag="PK016_05860"
     CDS             10828..>11111
                     /locus_tag="PK016_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXL02628.1"
                     /translation="MKVNFDLKGKAAVITGGGGILCSEMARGLASWGIQVAVLDIKED
                     AALKVAKEIEQSGGKAIAISADVLNKNSLKEARSKVLSEYGKIDILINGAG"
BASE COUNT         3004 a         1870 c         2749 g         3488 t
ORIGIN      
        1 tttcaaattt gaatacggac acacctgcgc gccctcccgc ttcatatgtg ctacttgtag
       61 aaggctttga taaggaaatt tcttgagaat ccttcttgga tctgattgtg accgacatgc
      121 ttggagcctt aattgcttct tccatttttt ctccttttat caaaaagcgg ttttgttctg
      181 atcacaaata aattgtatta ttgcttttca taataacata ttatttttat tgttgcaagt
      241 atctctaaaa tgatatagtt cccttgacag agtttataag ggatgctata tttattttaa
      301 aagttgctga tatattagat attagatgtc acaaagaagg aggaggtgat gttttttctg
      361 actgacattt ggtttaaaaa gagaagcttt aattttttag aaatcttgag aaaggaatga
      421 gcaaattgag aaaatttctt atgcttgcct tggtatgcgt tctgtttttg agtgtttcgg
      481 gggttgtttg ggctcaagca tggccagaaa aggaggtaga aataattatt ccctgggaag
      541 cagggggagc caccgatgta ttgtatagag cagttgctaa gtattttccc aaatatgcta
      601 atggcaaaca attaattatt aagaatgtgc ctggaggagg atcggctata ggttatggtg
      661 aaggcgccaa agctagacct gatggttata ctctggttgc tgctgttaac cctgttatta
      721 ccagagcatt aatggggcct gctccttatc atcctattga aagttttgat ccagtatgtc
      781 tgctggtaaa taatccctgc tatatactgg taaatagtaa attaaaaaaa tggaacagtc
      841 tttccgagct ggttgagtat gtcagggaaa acccgggagt ggtaacggta ggtaacagcg
      901 gtgctggtgg tggtaatcat ttggtggcat tgagttttga gaatgctttt gatctgaagt
      961 tcattcatgt accctttgat ggcggaggtc cttctgttaa tgctttactt ggagggcata
     1021 ttgatacagt agtagcttct tctcctgagg gtttccctaa tgtggaagcc ggtgagctgg
     1081 aaatgcttgc agtttttgct gaagagcgac ttgagaaatt tccccaattt cccacagctc
     1141 aagaacaagg ttatgacttt acggcattca tgtggagggg tgtggtagtt cccaaaggag
     1201 tagatcctga aattgtagaa cagattgctg aagtttttaa ggcagtgctg gaagaccctg
     1261 attttcagaa ggatgccgaa aacttaggac aaaatgcgct atatcttggc ccagaggagt
     1321 tcggaaaaca ccttgtttcg gagtttgagc gttaccaaga gattatcacc aaatatgggc
     1381 taggagaatt ttacaaatga gcttacagtg tctacagtgt cccctcgcag gaggggacac
     1441 tgaggaggta tcgcaaaatg gggaaagctg atattataat tggattttca cttttgtttg
     1501 tttcggggtt tcttctaata gaggcgatgg ggcttcctca aagccacgtg ggtataagtc
     1561 ctggtttgtt tcccgctatt gctctggggg gtttgatggt tttgaaccta tttttaattt
     1621 taaggggatt gaaacaaaga agggaaaagg cgcagtctta ttctgcttct ttaaacttaa
     1681 atttcgaaaa aattaagaaa atcgcgattt ttgtggcctt tgcctggctg tatatttttc
     1741 ttttacctct tctcggattt gtttatgcat caggaatttt tttctgggcg atttttattc
     1801 ttgccggagt aaatcaatgg ggtaaagctt taatcctttc ttttgggtcg agtatcataa
     1861 tgtacttcgt tttttatcga atttttatgg tcgctttacc tcgacctgct ttaccaattc
     1921 cctatccttt ttgagataga tatttctgga ggtagaaaat ggatgtcatt cagataagcc
     1981 ttttggaggg attgagacaa attcttgact ttcagactct cttgtatatt tttggaggag
     2041 tggttttcgg ctttatagtt ggtgctttgc ccgggctttc agcttcaact ggtattgttc
     2101 tcgttttacc tcttgtttat catattccag ctgcggaagc ggtaatgatt atgctcggaa
     2161 tatatggagg aactatgttc gctggttcca tacctgcaat tttgattcgt acgcccggaa
     2221 ctccttctgc tgccgctact gtccttgacg gttatcccat ggcccagaga ggagaagcag
     2281 gtcttgctct ttcaacttct gctattgctt cttttgtagg gagcctgctc agtgcagtag
     2341 ctcttttctt gattgctccg caaattgcca gggtagcact caagtttggc ccaccagaat
     2401 ttttttctct ggtggttttt ggcctcaccg ttattgctag tgtaaccgga aaagatgtca
     2461 ttaaaggttt aatctctgct ctgctggggc tctttctagg tactatggga atggatccca
     2521 tatcgggtta ttcccgattt acttttggtt cttatgctct tgaaggaggc ttaccgcttt
     2581 tgccggtgat gattggtctt tttgcggttt ctcaagtgtt gagagacata gaggaggcag
     2641 gagtaactta ccaggtgatt aataaaatag gacgaatttg gccttcctgg gaagaactga
     2701 aaagaatttt aggggcttct ctctttggat caacgctggg tacattaatt ggcgcgattc
     2761 ccggtattgg tggttcaata gccacttttt tagcatatgg tcaatatcgc aagttctcca
     2821 agagaggaga agagttcggt agtggagtat tagagggagt tgctgttcca gaagcagcta
     2881 ataatgccac aactggagga gctcttattc cccttttaac tctgggtgtt ccgggagata
     2941 ttgttaccgc ggtgatgctg gggattctaa ttctttttgg agtgaatcct ggacccatgc
     3001 tcttccggga acaacctgaa gtagttaata ggctttttgc atccatgttc gtgattgctt
     3061 tttttatttt agtgttgggg ttgtttggag cgcgattttc tccaaaaata ctcgccattc
     3121 ccaaaaaaat tctttctcct ttagtacttt tgttatgtat agttggggct tatgcagata
     3181 gttcaagtac ttttggaatt acagttgctc tggtatttgg agtgatgggt tattttatgg
     3241 aaaagtttga ttttccagtg gctccattgc ttatcagtat gattcttggt cctattgctg
     3301 aaggagaatt aggtcggagt cttctgattt caaggggtga ctggtcaata ctctttgttc
     3361 gtcctatttc gctttgtttt ttaattctgg caggattatc gctgttcttt gcctttcgaa
     3421 aaaaagatta gaaagaggtg gagactatgt tagagaaaaa taatctggat tcttccgtaa
     3481 agcaggagat tcaagaaaag ttagttcgtc ttcgcaagct tatagaagga aaaaatttgg
     3541 aggccttact tttaaaaagg catgtaaatt tcagttggtt gactgccggt gggactaatg
     3601 tcctctgtat ggctcaagat actggagtga gtagcatctt ggttactgat gacagaagct
     3661 atgttatagt cagcaatatt gaggcccccc ggatgaagga ggaagagaaa gtagttgcaa
     3721 aagggtttga acttgtggag tatccctggt atgaaggaaa tgaaatgaaa attgtgcgag
     3781 aaatctgcgg agaaggtagt attggttgtg atattgcttt tccaggaacg caagatttat
     3841 caggagaaat taatcgctgt cgttatcagc ttctggaaac agaaaaggaa cgttatctct
     3901 ggttaggtaa ggaagtttcc tggacactcg aagaagtact gtgtagtttg catcctggtg
     3961 tgcttgaatc ggaggtagct gcagaaattg ctctcagact ctggaagaaa aggattgaac
     4021 ctattggact ccaggcagct gctgatgaac gtgcttattg gtttcgccac ccggtagcct
     4081 ttcgaaaccc ggctaaaaag ttattattgg caagcgtcat ggctagatat ggtggtttgg
     4141 taaccacggc aacccgcatg gtttattttg gaacaccttc gccagaattc ttgaaacaat
     4201 atcatgataa tgtacgcatc gaatgcgaga tgatcgctct ctcaagacct ggtaacaaag
     4261 ctgtactccc ccttcaaagg gcagtggagc tctataaaga actgggctat cctgaggagt
     4321 ggaaacatca tcatcaagga ggagcaatgg ggtacttgcc tcgagattat cgggtagatt
     4381 ttgagtgcca ggaaaatatt ctcgaaaacc aagctttttg ctggaatcca tctatagctg
     4441 gtactaagtc tgaagatggc tttatttcta cttctcatgg tccgctattt ataacttacc
     4501 cggtacgttt tccggttttt agtctggaag tgggagattt tcattttgaa cgaccagatt
     4561 tacttgtact ctaaggagag taagctatgg gccataagcg aataggattt gcaattgttg
     4621 gttacggtct tgctgccaaa cttcacgctc aagctcttac tcagcttgat gaggcgaaac
     4681 taatagctat agtgggtagg aacattgaaa aagcttctga actggctaaa aactttggag
     4741 tagaagctct ttccctcgat gaagctttga agagggaaga cattcaagct tttattgtaa
     4801 ctacccctac aggtagtcac cttgaagtag tgagagaaat cgctcctcgt ggtaaacacc
     4861 ttttagtgga aaaacccctg gaaagcaact tacggcgtac tgatgagttg attcgaactt
     4921 gcgaggaagc aggggttatc ctgggtgctg tttttcagca tcgctatgat gatgcttctc
     4981 ttatgctcaa agagtataaa gataaaggcc ttctgggtag ggcagcaatg ggaagtgctt
     5041 atgttaagtg gtatcggtcg caggagtatt atgatgctcg ctcttggaga ggacgcgtag
     5101 aaacttccgg tggtggagca cttgctattc aggcttcaca tagcatagat cttttactct
     5161 ggtttttagg tgaacctgag aaagtatgtg gttttatgaa tactctggct catacgggaa
     5221 tagaagttga agatgcagca gtggtttcta ttcagtttaa aaatggagct ctgggtagca
     5281 ttgaagctag cacagctacg tatcctggtt ttccagagag actggagctc cattttgagc
     5341 gaggaagtgc catcattgaa ggaggtaaaa tagtttacct caacactctt tctgagaaaa
     5401 taccggttat agagcgagga cagagtggtc tatctggagc aagcgaccct gctggtgtgg
     5461 atattcagcc ttttgtaaga atgctccagg actttgttaa tgctctgaga gaaggcaaga
     5521 atccccccct ggatggtcga gaaggaagaa aagcagtgga gctgattgag gcagtacgaa
     5581 tatctgctct ggagagcaga gtggtagaac ttcctcttta acaagatgtg ggttatccta
     5641 tcatggttta gtgtagagcg cttccaggta taaagtagtg caatgtatat attagatacg
     5701 aatataaata atgctgcttt gtacctgata aaaataaaat aaaggaggag tgggtagtat
     5761 gactgaggga tgtttaaaag gggtatttgt actggtggta ctgcttgttc tggttttagg
     5821 gatgacttcc cttgctttcg gggaggtaat tcttaaagtg gggagtccct tcgaagaagg
     5881 tcatatcttg gtagaggcgg caacaaggtt caaagaactt cttgaagagc gaagtgagga
     5941 aagattacag gtagagctac agatagctca agcttcagag gaggaggtaa acacccagtg
     6001 ttcggaagga gtaattgatc ttcagtttaa tggaggccgg ccggtggaag tatttgctcc
     6061 gcaatacttc ttcttcaacg caccttatgt tatcaaagat tacgatcatt ttctgagagt
     6121 atggaacggt cctcttggcg aaaaagccaa ggagctggta cttcaaaatg gcaatatgtt
     6181 ctgtttgggt acggtgtacc gtggtttccg gcaaatgact tcacttaatc ctatcaggac
     6241 cctcgaagac ctggtgggtt taaaactgcg ccttccggta atcaaaacct ggattgcagt
     6301 ttgggagtca ttaggagttg agccggtgcc agttccactt ccagagctgt atcaggcact
     6361 taaagaaagg aaagccgaag cttcagaagg agatcttccc cagatatatt ccttcaaact
     6421 gtatgaagtt caaaagtatc tgggaattac taatcatctg gttggtgtgg gctggatagc
     6481 tatgaaccgg aattccatgg aacaactttc tcccgaagat caggacctgg ttaggggatg
     6541 tgttgaggag gcctgtcagt gggccacgga aaagattaaa actggcgaaa gcgacttgct
     6601 tgcagaatta caggaacttg gtatggaaat tgtttatcct gatgcggatg ccataaggga
     6661 gaaagccaag ccagctattg aagaactgtt tgttactgag tggccggtta ccacttggga
     6721 agaggtgctt gctcaataag tattcggcgg agcaggttgc tccgacttgc tccgcttcct
     6781 gtctgttgat ttatattgca gttatggttc tcagcttcct tctgtgttcc gaaaatttca
     6841 aattaaatct tgaacttttt gccaggtttg ataaaattag aatgtgatat atcaggtatt
     6901 acatttcaca atctaatggt ttctttaaca attggaaaat tgctggtttg gacaaaatag
     6961 gaattttcca taaattaagg aggtgattaa aacatagata gtaaaatctt ctcaagctct
     7021 tgctttgaag ctttttatta atttaacatt agcttaactt tgaaaaggga ggtttagtga
     7081 gatgcttaaa aaactaactg gagttatcgt tgtgttagcg atgttagtgg gattttgggc
     7141 ttcagttgcc ttagctcagg aacaatatgt tttgaggttt aatcacgtgt tggctcccgg
     7201 tcacccctat catgaaggat tgcaaaaatg ggctgagcgg gtatatgaaa gaaccaatgg
     7261 aggtttgaag attgaggttt tccacagtgc tcagttggga gtcgaagaag atatcataga
     7321 gcagatccgt caaggtgttc cggtaggaca aaatactgat gctgctcgcc tcggtaacta
     7381 tgtacctgaa attgcagtaa taaacggtcc ttattttgta gaaagtattg aagaggtcca
     7441 aaaacttcat gaatctcctt cgattcaagc atggatagat aaactggata aagaatatgg
     7501 tcttaaggtg ctatccttta attgggtgca aggttacagg cactttatga ctaaggataa
     7561 gcaagttaga actcccgagg atttaaaagg tctgcgtatc cgaactgctc ctgcccccat
     7621 ctggcaggaa tctgttcggg cactgggagc aacccctact gctttgaatt ttgacgagat
     7681 atatactgct ctacaacaag gcgcaataga tggagcagaa ttagtgtatg ataatgttac
     7741 ctcaggtaac ctttatgaag ttctcaagta tatcagcgaa actcgtcatt tcttgctggt
     7801 gaattttgaa gtagtaagta gtaaatggtt ctacagtctt cctgaggagt atcaaaaaat
     7861 tttagtagag gaatgtgacc gggcaggctt ggaaacttct tatttgatcg aggaaaatac
     7921 cgagaaaaac aaagaaataa ttcgagaaaa aggtgtaact ataattgaag atgttgacct
     7981 ggatgcattc agaaaagctg gagaagcagc ttatgaggtc ttaggcataa gtgaagccaa
     8041 aaataaagtt tatcaagaaa ttggaaagta gtagttagag tttgtattta cccccaagcc
     8101 agcctggctt gggggtaaat agggtataaa cagagttgga aattgattat aaataggtga
     8161 taaaaatatt tgatgagggg tatcgataat gaccatcaaa aaaatctatg acctaatcgg
     8221 tagaggagaa gaggtagcag cgggaattct tatggttgca attatggtgg tggttttcgc
     8281 tgcggggttt ggaaggacta ttggttaccc tatcccatgg ggaatggaaa tgtctacttt
     8341 tctttttgcc tgggcagttt ttttcagtgc tgatgctgcc tggagaaaaa atagacacgt
     8401 aagtgtagat attttagtta acaaatttcc agaaaaagtc cggtattata ttgctctagt
     8461 caaccacctc ctcatttttg gattcttggt gcttttgatt ggatatggta tccccctttg
     8521 ttaccatacc agattccggg cttttcaggg tattcccggg tttagttata tgtgggctac
     8581 tctcagcgta ccagtaggaa gtgctcttat cttaataacc acctctttaa ggataagaga
     8641 acttataaag aacggtaaaa ttacaatttc tgaagtcgaa agcgaagaag gtatggattt
     8701 ggatgtagag gattagatat tgtcactcgg tagggaggaa aaagtatgct attagtatcc
     8761 atcgtattta ttgttctttt gttgctcggt atgccggtgg cttttgccat aggtatttca
     8821 ggctttttat tttttgttca acagccagag ttaccactga ctatgccagt ccagcttatt
     8881 ttatctggaa ctcagagttt ttctctgctc gccataccga tgtttatttt tgctggtaat
     8941 ttgctaaata ataccgggat aaccagccgg ctggtaaaat ttgcttcagt ggttaccggc
     9001 catatgcggg gtggactggc ccaaaccagt gtagtattaa gtactctgat gggaggagtt
     9061 actggttcat ctatcggtga tgcttccatg gaagccagaa tattgggacc agcaatgact
     9121 caacggggtt atgatcgggc atattcagct gcagtacatg gttttacttc cttaataacc
     9181 atagccattc caccggggat tggacttgtg ctatatggaa gtattggtga agtctctgtg
     9241 gggcggctat ttgctggggg tattttaccc ggacttttga tggcggcttt gtttatggta
     9301 gtggtctccg ttacttcagg taaaagaggt tatcttcccg agcgagaaaa gagagcccct
     9361 tttaaagaga ttgggctatc ttttattaat agtctctggg ctatattttt ccctattttt
     9421 ctcttggtag cgttgcggtt tggtttttta attccttccg aagcaggtgc ttttgcctca
     9481 gtatatgccc tggctgtggg tttgatagct tatcgagagc ttacctggaa gaaatttgtc
     9541 tctgctatta agtacacctt gatggatgta ggcatggtta tgtttttaat cgctctatcg
     9601 tctctgatta gttatggcat gacctgggag atgatacctc agactatttc tcagtttttg
     9661 ttgggaatta gtgaaaatcc ctatataatc actggtctaa ttgtgctatt acttttagtg
     9721 ctgggtatgt ttatagattc aacagtttta attttgttgc tcacttcgat attggtaccg
     9781 gtaatgagcg agataggaat ggacctggtt cattttggag ttttgatggt aataacttgc
     9841 gctacagggc ttttaactcc tccagttggt ttgactatgt atgccgtttg ttcgattatg
     9901 gaatgttcac tggaagatta catgagagaa gcttggccct ttctgatagc tatgtttatc
     9961 ttaattgcac ttatattgtt cttcccccaa attgttttat tcattcccaa cttaatattt
    10021 ggctaaaggg cagataaaga ttttcaggag gtatcgttta tggaaggaaa ggtagcaatc
    10081 gttaccggtg gtgttgctgg tattggtaga gctattgctg accgcttggc tgagcgaggt
    10141 tatacggtta tagtatgtga cattaaccga gaaaaaggag aagttctgga aaaagccaac
    10201 cataaggtta aaggagctta tgtattcaaa tattgtaatg taggcgaaga agaagatatt
    10261 aaaagagtag tagaagaagt agaaagagac tacgggggaa tttcagtgct ggttaataac
    10321 gctggtatta tccgaagacg taaagaggga gaaattaaaa tatctgactg ggatgatgtt
    10381 tttaagatta atactcgagg cacttttatt ttctgcaaat atgtttctga agttatgaaa
    10441 aagcagaaaa gtggccgaat aataaatatt tcttctgtag ccagtaaaat gggcgacatt
    10501 acctcagctc ctggatatgg tccttctaag gcagcagtaa atggacttac taaaaccttt
    10561 gcccgggagt tagcccctta caatataacg gtaaacgctg ttgctcctca cgccattgaa
    10621 actgaaatga gcgctgagtg gtcggaagag atgagacaaa aaatcatttc tgaaatcccc
    10681 cttaagagat tgggtaaacc atttgaggta gcggcagtgg tggagttttt gatttccgat
    10741 gaggctggtt ttattaccgg agagataatc gacgttaatg gtggcttttt aatggattag
    10801 ggaaattaaa agaggtgata acaaataatg aaggtaaatt tcgatttaaa aggaaaagca
    10861 gctgttataa ctggcggtgg cggaatcctt tgcagtgaaa tggctcgcgg tttagcttcc
    10921 tgggggattc aggtagcagt gctggatatc aaagaggatg cagcattaaa agtagcgaaa
    10981 gagattgaac aaagtggtgg gaaagcgata gctattagcg ctgatgtatt gaataaaaat
    11041 tcccttaaag aggcccgctc aaaagttttg agtgaatatg gcaagataga tattttaatt
    11101 aatggggcgg g
//
LOCUS       DAOSVE010000058        10907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_3371_length_10907_cov_65.952820, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000058 DAOSVE010000000
VERSION     DAOSVE010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 10907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10907
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3371_length_10907_cov_65.952820"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="PK016_05865"
     CDS             complement(<1..1015)
                     /locus_tag="PK016_05865"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXL02629.1"
                     /translation="MEIKLVPREDCEHALKLVTYCFPWFSEIGEQARELIDEFVSKER
                     ILGHYDEYGFLNAMIAILPFNIYIYGSPLGMGGIGIVSSMPEGRHGGNIAELLKNSLQ
                     IMRSRKQFVSMLGPFSYEFYRKYGWELSFDRLNYNIPIDHFSKFNKKLGFMKPFKDND
                     LETLNAIYTEYARKHNCCVIRDKTLWTDFILNDFRVKSCPKYTYLWADADNNPGGYIV
                     YTIKENKMNIHEMVYLDQQAKEGLLWFIFAHQSQVDNVRWSTSSDERLYLDLANPRVG
                     MELSPGMMFRVVDVKNALLSRDYDKDIEAKFSISISDPNAKWNNKKFLIELEEGKINV
                     EECE"
     gene            1354..1596
                     /gene="csrA"
                     /locus_tag="PK016_05870"
     CDS             1354..1596
                     /gene="csrA"
                     /locus_tag="PK016_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HXL02630.1"
                     /translation="MLVVTRRVDQSIRIGNNVEIKILDIEKGKVKVGVSAPPDIPVYR
                     EELYQSLVQDNQKALFISEEMVPTLRKVFSPPESAD"
     gene            1734..2504
                     /locus_tag="PK016_05875"
     CDS             1734..2504
                     /locus_tag="PK016_05875"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXL02631.1"
                     /translation="MKVKKELFLAIITFLLLSSGIHTIGAKEAPSFQLPGLDGEIYSL
                     SDFRGEPVVISFSATWCPPCRQELPLLQKMYEEYKDKARLNVLVIDSEEPQSKVEAFA
                     QELGLTFPVLLDEEGKVAREYGILYLPTLCFVDPLGNLAGQIIGAQTESVLRSKLDQV
                     LWFRGLQEVEVKNLVELTSEIVILDFRKEGTNPFPDRQNISYQMVGSVDSVISSAPRE
                     KTYLLLTESNEEGVEIGKALAISGFQRVYYWMVDAPQS"
     gene            2488..3045
                     /locus_tag="PK016_05880"
     CDS             2488..3045
                     /locus_tag="PK016_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02632.1"
                     /translation="MLHRVDWSLATLVFVSLFFLVGVVYVWWTGELPFFPQEVLEGES
                     SFSQIQMEKAEISRVGEKGKEWVLNAHSVEQKGDDVFLIDVSGVFFQEGIPLYQVEAQ
                     KGQIFLSEGDVELQKVKLINEEKKETLQGELLLWRGKEEKFELSEAQFAGQGIEASCQ
                     QIIYNISKKKLLFENNVELKIKVEE"
     gene            3042..3728
                     /locus_tag="PK016_05885"
     CDS             3042..3728
                     /locus_tag="PK016_05885"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HXL02633.1"
                     /translation="MRKIFLEALILAFLFLFSPFLLAQEEETVLITTPQAEYNAETGK
                     IVAQVSTFTWRDVKVVCPFLEIDTKTQEARSEGNINISWGDFQVQAQSLYYQGDKNRL
                     DFSGIKGSNPEMNFSTGEAHFDFNQKKLYLTSSPLLILQGWELRAQGIEYSLEEKTWE
                     AQVVRVEKEAWQGRAEKAFYREEDDFITLEGGAEAQGDGNLLRGEKILIYLDSGRVKV
                     EGNVEIELVP"
     gene            3751..4485
                     /gene="lptB"
                     /locus_tag="PK016_05890"
     CDS             3751..4485
                     /gene="lptB"
                     /locus_tag="PK016_05890"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HXL02634.1"
                     /translation="MEKNWNALLGRGLTKEYGKKRVVDEVDLEVYRGEIVGLLGPNGA
                     GKTTTFYLLVGLVRPTGGKVFLDALELNHFPMYLRARMGMSYLPQEPSVFRKLSVKDN
                     LDLVLEMQGLSKKEVKEERERLLREFGISHLANSSANLLSGGERRRLEIARTLATFPS
                     FVLLDEPFTGIDPIAVEDIQNLVKYLASKNIGVLITDHAVRETLAITDRAYIMFEGKI
                     LVSGTSEEIINSDLSRKYYLGERFNL"
     gene            4488..5564
                     /locus_tag="PK016_05895"
     CDS             4488..5564
                     /locus_tag="PK016_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HXL02635.1"
                     /translation="MLDKYILKQSVVNFLFGVILFVILVSAGDLLFRLARLWLQGGLS
                     LGKVIKVFFLSLPSLLIYILPVSVLLSVLLTVGRMSGDSEVVALRAGGISFRRLTIPF
                     FLFSLWVCAGTIIIQEACLPWASVELKQMEEEVSGGGVIPENTFFRDDSEPGIERMFY
                     VKTIDPEKFLLLGVVVQEYDEKGLRRIINAQEASNQEGKWIFKEGIIYEVGEGGEIER
                     VVRFSKEEVTTTQSIEEIQKIRKSPQEMSFLELKNFIEGERSRGRKTEKLELILWQKT
                     AFPFASLVFVLIGVSLGIASPRSGRALGIGLSILIVFAYYVIFSLGSTLAEGGGLSPF
                     WGTWIANIMGFAIGGSLLWWREKS"
     gene            5577..6614
                     /locus_tag="PK016_05900"
     CDS             5577..6614
                     /locus_tag="PK016_05900"
                     /inference="COORDINATES: protein motif:HMM:NF022727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3084 domain-containing protein"
                     /protein_id="HXL02636.1"
                     /translation="MNWGSWLLVFSIIVLAGVIAYLGDILGRRIGKKKLSLFKLRPRY
                     TAILMSVITGILIAAITLLILSIASQDVRTALFGMEKLKSQITQLEEREKELQSYKAS
                     LEEQVKTLTGSVEELKSQRQSLQEEIQTLQVELTRLRESILAIRRGEIVFQDEEEILR
                     VVAPKGMDDKEADNYLLSIIKQGEDIALQRGAGLDKEIQRAVFVLEDNFNETKEKLKN
                     SEVPLVIRLVASINTLRGEPVIARFITEENKKVFSAGEVIWSGELNIDPQKTNIELWL
                     GELLKELNTLAVKKGILPQQGRVGVISASNITEVTSLLQRMEGKVTVEVVAESDVFTA
                     GPLRIYLRVKQ"
     gene            complement(6597..7499)
                     /locus_tag="PK016_05905"
     CDS             complement(6597..7499)
                     /locus_tag="PK016_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HXL02637.1"
                     /translation="MYQVLTVMFLLIALGMFLRAVKVFPPETAPLLNRFVLYLTFPCL
                     IFRSLQPAHLAPELWKIPPVAYTLISLVFIASYFLSSRLLHLRDETSVSLAMGAAFGN
                     TAFLGYPFILALLGERGLPPAIFFDQMGNFLSTYTVGVLLCVYTQTKRLSARSLLEII
                     KLPPFLAFLVAIITNGLTIPPFLWETINRLADATVPLTMVAIGMSLSASHLFRNWKPL
                     LSASLIKLVVLPLLFLVCTKYASFSPLYQKVMVIQAATPTLVTSYTLASIYKLDSEFS
                     SSVIFFTTFLSLFALPLWGLLLHP"
     gene            complement(7501..8262)
                     /locus_tag="PK016_05910"
     CDS             complement(7501..8262)
                     /locus_tag="PK016_05910"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HXL02638.1"
                     /translation="MQIARFLHPEKKIPLFGVIKGDIIEEISDPFGEIKEEGRVFSRG
                     TVKLLSPTLPSKIVAVGLNYRHHAQELEERIPENPIIFIKPSTAVLDPDDFILLPSSS
                     CQVDYEGELAVVIKKKAYKVKDSHFGEYVLGYTCFNDVTARDLQKKDGQWTRAKSFNT
                     FAPFGPWIETEIEPGNLNIRTYVNGYKKQDSNTKELLFPVPMLISFISEVMTLLPGDV
                     IATGTPEGVGPLHPGDIVEIEIENIGILRNPVKGE"
     gene            complement(8234..9643)
                     /locus_tag="PK016_05915"
     CDS             complement(8234..9643)
                     /locus_tag="PK016_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXL02639.1"
                     /translation="MNILLVNPPSPGVYNWIGLKLPPLGLAYLASTLEQEHHRIKIVD
                     LQVEPKKELQKNLQNCDLVGITCETNKAPEALKIAESAKKEGRLVVLGGYHATFCDEE
                     LLNTGLIDFIVRGEGEYTFSQLVKVLENNESPRSVSGISFREEGKIVRTPPAPLPQNL
                     DEIPFPQRRLLPLSKYWMTQVEGEPLVNMVTSRGCPFGCSFCASSQFSGKKWRARSPE
                     NVIEELRQVYFDLGYRAVAFVDDNFTLSPARAESICDKIEQEKMKIKWWCFSRADTIV
                     KHESLVQKMARAGLRMVYLGFESAEESILQDYGKNLTAEVSKKAVEVLRKYGVKSWGS
                     FILGNIKETKETIKKTVEFAKKINPDIVQFSILTPFPGTETYEKYQKEGRIINQNWKA
                     FDGAHPVLQLDFLNPQELHHFLIRAYLEFYERWSYLPQAFSFIKKFLVVNLPPISRIK
                     EAQYWKERLKNANSPVPSS"
     gene            complement(9668..10756)
                     /locus_tag="PK016_05920"
     CDS             complement(9668..10756)
                     /locus_tag="PK016_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HXL02640.1"
                     /translation="MPYRITLIPGDGVGPEITAVTKKVIEATGVEIEWDIQKAGAEVM
                     EEYGTPLPPSVIESIKKNKVALKGPITTPVGSGFRSVNVALRKELDLYACVRPCKTYP
                     GVRSLYQNIDLVIIRENTEDLYAGIEFEKGKPETLKLIEAIEKWGKGKIRPDSGISIK
                     PISVFGSERIVKFAFEYARSENRKKVTAVHKANIMKYSDGLFLAVAREVAKNYPDIEF
                     EDRIVDNMSMQLVQKPELYDVLVLPNLYGDIISDLGAGLIGGLGVAPGANIGDEYALF
                     EPTHGSAPKYRGMNKVNPLAMILSGMMMLKYIGEKEKAEKLEIAIKEVIREGKKVTYD
                     LKPTPDDPTACGTKEVGEAIIEKLKNLS"
BASE COUNT         3284 a         1956 c         2324 g         3343 t
ORIGIN      
        1 cttcgcattc ttccacattt atctttcctt cttctaattc gataagaaat tttttgttat
       61 tccactttgc attaggatca gatatagaga tggagaattt agcttctatg tctttatcat
      121 aatctctact taagagtgca tttttgacgt caaccactct gaacatcata cctggagaca
      181 actccatccc tactctcggg tttgctaaat ccagatatag cctctcatca gaagatgtag
      241 accagcgtac attatcaact tgagactggt gagcaaatat aaaccagagt aatccctctt
      301 tagcttgttg gtcaagatac accatctcat gaatgttcat tttgttctct ttgattgtat
      361 aaactatgta acctccaggg ttattatccg catcagccca taagtatgta tattttgggc
      421 aacttttaac acggaaatcg ttcagtataa aatccgtcca taaagtttta tcacggataa
      481 cacaacaatt atgttttctg gcatattcgg tatagatagc atttagtgtt tctaagtcat
      541 tatctttaaa tggcttcata aaacctagtt ttttgttaaa cttagaaaaa tgatcgatgg
      601 gaatgttata gttcagtcgg tcgaaagata gttcccaacc atattttcta taaaactcat
      661 aagaaaaagg tcctaacata gaaacaaatt gttttctact cctcataatt tgaagggaat
      721 ttttaagtag ttctgctatg ttacccccgt gtcttccttc tggcatagaa gatacaatgc
      781 caataccgcc cattccaaga ggagagccgt atatataaat gttaaatggt aagatagcaa
      841 tcattgcgtt taaaaatcca tattcgtcat aatgacccag aattctctct ttagacacaa
      901 attcatctat tagctctctg gcttgttcgc ctatttcact aaaccaggga aaacagtaag
      961 ttaccagctt gagtgcatgt tcgcaatcct ctcggggtac taattttatt tccaaactct
     1021 gtctctcctt taaattatgt acaaacaaat ttttttaatt ttaccatatt ctattctatt
     1081 ctcataaatt tatttatttt tagcttttct cttgcttttt gagagataat gctactttaa
     1141 tccgtttttc gtaagttcta ttcattagcg actatcggta caagttaatg taaggaaaac
     1201 taccaattta taatttgccc cattacctgg gtttctccag ttgtctgtac cgaaatatga
     1261 gaaggaagaa attagcactt gtttttcccg agctcctttt ataaaatgca atttaggtat
     1321 aaaatagaat agaaactttt aaaggagaag tttatgctag tcgttacccg aagagtggac
     1381 caaagtatca ggattggtaa taatgtagag ataaaaatcc tggacataga gaaggggaag
     1441 gtaaaagtag gagtatctgc ccctccagat atcccggttt atcgagagga actatatcag
     1501 tccctggtgc aagataatca gaaagctctc tttatctcag aagaaatggt tcctactctt
     1561 cgaaaggttt tttctcctcc tgaatctgcc gattgagcgt tttggtatgt tctttctatt
     1621 tatagttcta cctactatcg ttttcagaga taaatttcta taattatttc aggggaattt
     1681 ttaagggtta aaatgaatat ataaaaaata agaatagcag aaaggagaaa aacttgaaag
     1741 ttaaaaaaga actcttcttg gctataataa ccttcctttt attatcttct ggcattcata
     1801 caattggtgc taaagaggcc ccttcatttc aacttccggg gttagatgga gaaatctatt
     1861 ctctctccga tttcagggga gaaccggtgg taatttcttt ttctgctacc tggtgcccac
     1921 cctgtcgcca agagcttcct cttctacaaa agatgtacga ggaatacaaa gacaaagctc
     1981 gattaaatgt tttagttata gactcagaag agcctcaaag taaagtagaa gcttttgctc
     2041 aggaattagg ccttactttt cctgtccttt tagatgaaga aggaaaagta gctcgagaat
     2101 atggcatttt gtatttacct actctttgct tcgtagaccc tctgggtaat ttagctggtc
     2161 agataatcgg agcgcaaaca gaaagtgttt tgcgcagtaa actggatcag gttctctggt
     2221 ttcgaggtct ccaggaggta gaagtaaaga atttagtaga gttaacctca gagatagtga
     2281 ttctggactt tagaaaagaa ggtactaacc ctttccccga caggcaaaat atttcttatc
     2341 agatggtagg tagtgtagac tcagtcattt cttctgctcc ccgagaaaaa acttaccttc
     2401 tacttaccga aagtaatgaa gaaggtgtgg aaataggtaa ggctctggct atctctggtt
     2461 ttcagcgagt ctattactgg atggtagatg ctccacagag ttgattggag tctcgcgaca
     2521 ttagtttttg tttctctctt ttttctggta ggagtggttt atgtctggtg gacaggagag
     2581 ttaccttttt ttcctcaaga ggtactggaa ggagaaagct ctttttctca aattcagatg
     2641 gaaaaggcag aaataagtcg ggtgggagaa aagggtaaag aatgggtctt aaatgcccat
     2701 tcagtagagc aaaaagggga tgatgttttt ttaatcgatg tttcaggagt ttttttccag
     2761 gaagggattc ccctttatca agtagaagct cagaaaggac aaatattttt gtcagaggga
     2821 gatgtggaac tgcagaaggt aaagttaata aacgaagaaa agaaagaaac tctgcaggga
     2881 gagctgttac tgtggcgagg gaaggaagaa aaatttgagt taagcgaagc tcagtttgcc
     2941 gggcagggaa tagaagccag ctgccagcaa ataatttata atatttcaaa gaagaaattg
     3001 ttattcgaaa acaatgtaga attaaaaatt aaggtggaag aatgaggaaa atttttcttg
     3061 aagctttgat tctggctttc ttatttcttt tttctccttt tcttcttgct caagaagaag
     3121 agacagtatt aattactact cctcaagctg aatacaatgc tgagacgggg aaaatcgtcg
     3181 ctcaggtttc tacctttacg tggcgagatg taaaggtggt ttgtcccttt ttagaaatag
     3241 atactaaaac tcaagaagca agaagtgaag ggaatataaa tatttcctgg ggtgattttc
     3301 aagttcaagc tcaatctctc tattatcagg gcgataaaaa tcgattggat ttttcgggaa
     3361 taaaggggtc taacccggaa atgaactttt ctactggaga agcccacttt gatttcaatc
     3421 aaaaaaaact ctatcttact tcatctcctc ttttaatttt acagggctgg gaattgcgag
     3481 cgcagggtat agaatactct ctggaagaaa aaacctggga agctcaggta gtaagagtag
     3541 aaaaagaggc ctggcagggt agagcagaga aagcttttta tcgagaagaa gatgacttta
     3601 taactctgga gggtggagca gaggcacagg gagatggtaa tcttttgcga ggagaaaaga
     3661 tacttattta tctggatagt ggccgagtaa aagtggaagg aaacgtagag atagaactgg
     3721 ttccctaaaa ggaggaaatg tgtttgtcca atggaaaaaa actggaatgc tttattagga
     3781 agaggcctga ctaaagagta cggtaaaaaa agggtagtag acgaagttga tttagaagta
     3841 tatagaggag agatagttgg tcttttagga cctaatggag cggggaaaac tactaccttt
     3901 tatcttctgg taggtctagt aagacccaca ggaggaaagg tttttttgga tgctctggag
     3961 ctcaaccact ttcctatgta tttaagagct cgtatgggta tgagttatct tccccaggag
     4021 ccttctgttt ttcgaaaact ttcagtgaaa gataatttag acctggttct ggaaatgcaa
     4081 ggtctatcta aaaaagaagt gaaagaagag agggagcgtc tcctaagaga gtttggcatc
     4141 agccatctgg ctaattcttc tgctaatcta ctctctggag gagaaagaag gcgcttagag
     4201 attgctcgaa cgctggctac ttttccttcc tttgtccttt tagacgagcc atttactggt
     4261 attgacccta tcgccgtaga ggacattcaa aatttggtaa aatacctggc cagtaaaaat
     4321 attggggttt taatcactga ccacgctgta cgagaaacgc tggccattac tgaccgagct
     4381 tatatcatgt tcgaaggcaa aattctggtt tctggtacct cagaggaaat cattaattct
     4441 gatttgagta ggaaatacta tttaggagaa cgttttaatc tttgaaagtg ctggataaat
     4501 acatccttaa gcagagtgtg gttaactttc tttttggagt tattttgttt gttattttag
     4561 ttagcgctgg agatttactc tttcgtttgg ctcgcttatg gttacaagga ggtctttctc
     4621 tggggaaggt aataaaagta tttttcttaa gtcttccctc tcttttgatt tatattttac
     4681 cagtttcagt attgttaagc gtattactca ccgtaggtcg catgtctggt gatagcgaag
     4741 tcgtagctct gagggcagga gggattagtt tcaggaggtt aacaatcccc ttctttcttt
     4801 tttccctgtg ggtttgtgcc gggacgatta ttatccagga agcatgttta ccctgggcct
     4861 cagtagaact caagcaaatg gaagaagaag taagcggagg aggggtaata ccagaaaata
     4921 cttttttccg ggatgacagt gaaccaggca tagaaaggat gttttatgta aaaacgatag
     4981 acccggaaaa atttcttctt ttaggagtag tggttcaaga atacgatgaa aaaggcttga
     5041 gaagaatcat caatgctcaa gaagcttcta accaggaagg aaagtggata tttaaggagg
     5101 gaattatcta tgaggtaggg gaggggggag aaatagaaag ggtagtacgt tttagcaaag
     5161 aagaggtaac tactacacaa agcatagaag aaatacaaaa aatcaggaaa agtccacaag
     5221 aaatgagttt tctggaatta aaaaatttca ttgagggcga gagaagcagg gggagaaaga
     5281 cggagaaact ggaattaatt ttgtggcaaa aaactgcctt tccctttgct agtctggttt
     5341 tcgtcctaat aggagtttct ctgggtatcg cttctcctcg ctcgggtcga gctctgggaa
     5401 taggattaag tatcctcata gtttttgctt attatgttat tttttcttta ggtagtactt
     5461 tggccgaggg aggtggtttg tctccctttt ggggaacctg gatagctaat ataatgggtt
     5521 tcgcaattgg tgggagtttg ttatggtgga gagaaaaaag ttaaaggaga ctttctatga
     5581 actggggtag ttggttattg gttttttcta ttattgtttt agccggagtt atagcttact
     5641 taggagatat tttaggaagg agaattggaa agaaaaaact ttccttgttt aaacttcgtc
     5701 ctcgctacac tgccattctc atgagcgtta ttactggaat tttaattgct gctattactc
     5761 ttcttatttt gagcattgcc tctcaggatg tgcgtactgc cctatttggt atggaaaagc
     5821 taaaatctca gattactcaa ctggaagaaa gagagaaaga gttacagagc tataaagcct
     5881 cgcttgaaga gcaagtcaaa actttaactg gaagtgtgga agaactaaaa agtcagagac
     5941 agagtctcca ggaagaaata caaactttac aggtagagtt aacccgtctg cgggaaagta
     6001 ttctggctat ccggcgggga gaaatagtat ttcaggacga agaagaaatt ttgcgagtgg
     6061 tagctcctaa aggcatggat gataaagaag cagataatta tcttctttcc ataattaaac
     6121 agggggaaga tatagctcta cagcgaggag caggccttga taaggaaata cagagagctg
     6181 tttttgtctt agaggacaat tttaatgaga ctaaagagaa gttaaaaaat agtgaagttc
     6241 cattggttat tcgtttggtg gcctccatca atactctgcg aggggaaccg gttatcgctc
     6301 gatttataac agaggagaat aagaaagttt tctctgccgg agaagtgata tggtctggag
     6361 agctgaatat tgaccctcaa aaaactaata tagaactatg gctgggtgaa ctattaaaag
     6421 aattaaatac tctagcagtt aaaaagggaa ttctacctca acaaggtagg gtaggagtta
     6481 tctctgcttc caatattacc gaggtcacct ctcttttaca aaggatggaa gggaaggtca
     6541 ccgtggaggt agtagcagaa agcgatgtat ttactgctgg gcctttgcgc atctatttac
     6601 gggtgaagca gtaaacccca taaaggcagg gcaaaaaggg ataaaaaagt agtgaaaaag
     6661 atgacactgg aagagaattc gctatctaat ttataaatgg aagctagcgt gtaagaagtc
     6721 acgagggtgg gggtggcggc ctgaataacc atgacctttt ggtaaagagg gctaaaagaa
     6781 gcatatttag tgcagaccaa aaacaaaaga ggaagaacta ctaatttaat aagagaggcc
     6841 gaaaggagag gtttccaatt tcgaaagaga tgagaagcag acagggacat tcctattgct
     6901 accatagtta gaggaacagt tgcgtcggcc agacggttaa tggtctccca aagaaatgga
     6961 ggtatggtta aaccgttagt tataatagct actaaaaaag ctaaaaaagg gggaagtttt
     7021 attatttcta aaagacttcg agctgataga cgtttagttt gggtatatac gcagagaagt
     7081 actccaaccg tgtaggtaga gagaaagttt cccatctggt caaaaaatat agcaggtggc
     7141 aaacctctct cccctaaaag agccagaata aaaggatatc ctaaaaaagc agtgttgccg
     7201 aaagctgctc ccatggctaa acttacgctg gtttcatctc ttaaatgtaa aagtcgggag
     7261 gataaaaaat aagaggcaat aaaaactaag ctaatcaagg tgtaagcaac aggaggaatt
     7321 ttccatagct ctggagcaag atgagcaggt tgtaaagaac gaaatattag acaaggaaag
     7381 gtaagataga gaacaaaacg atttaaaagg ggagcagttt cagggggaaa aacttttacc
     7441 gcccgcagga acatccctag agcgattagg agaaacatta ccgttaagac ttggtacaag
     7501 ctactctcct tttactggat tgcgtagtat cccgatattt tctatttcta tctctactat
     7561 atctccggga tggagtggcc ccactccctc aggagtcccg gtggcaataa catctcctgg
     7621 caataaagtc ataacttcag aaataaaaga gataagcatg ggtacaggga acaggagttc
     7681 tttagtgtta gaatcttgct ttttatatcc attgacatag gtgcgaatgt tcagatttcc
     7741 cggctctatt tcggtttcta tccagggacc aaaaggggca aaagtattga aagattttgc
     7801 tctggtccac tgcccgtctt tcttttgtaa atcccgggca gtgacatcat taaaacaagt
     7861 atatcctaga acgtattctc caaaatgtga atctttcact ttataagcct ttttctttat
     7921 tactactgcc agctctcctt cgtagtctac ttgacaggaa gaagagggaa gcaagataaa
     7981 atcatcgggg tccaagacag cagtggaagg tttgataaaa atgatggggt tttcaggaat
     8041 tctttcttcc agctcctgag cgtggtgacg ataatttaac cctactgcta cgattttaga
     8101 aggtagagtg ggggagagca gtttaactgt tccccgagaa aaaaccctcc cttcctcttt
     8161 tatttctcca aaagggtcag aaatttcctc aattatatcg ccttttatca ctccgaaaag
     8221 gggtatcttc ttttcaggat gaaggaaccg ggctatttgc attttttaat cgctccttcc
     8281 agtattgggc ttctttaatt cgggaaatag gaggtaaatt gactactaaa aatttcttta
     8341 taaaactaaa agcctggggt agataactcc atcgctcata gaattcaaga taagctctaa
     8401 taaggaaatg gtgtaactcc tgaggattaa gaaaatctaa ctgcaggaca ggatgggctc
     8461 catcaaatgc tttccagttt tggttgataa tccgcccttc tttctggtat ttttcgtatg
     8521 tttcagtacc agggaaggga gtgagaatag aaaattgtac aatgtcggga ttgatctttt
     8581 tagcaaactc cacggtcttt ttgatagttt ctttggtttc tttaatatta cctaagatga
     8641 aagagcccca acttttaacc ccgtatttgc gcaagacttc tactgccttt ttggatactt
     8701 cagcagtcaa atttttcccg taatcttgta gaatggattc ttcagcactt tcaaatccta
     8761 agtacaccat ccgcaatcct gctctagcca tcttttgtac cagagattcg tgttttacga
     8821 tggtatcagc ccgggagaag caccaccatt ttattttcat cttttcttgc tctattttgt
     8881 cacaaatgct ttctgcacga gcaggagata gggtaaaatt atcatccaca aaggctactg
     8941 ctcggtatcc taagtcaaag taaacctggc gtagctcttc tattacattt tctggagaac
     9001 gagctctcca ctttttacct gaaaattgag atgaagcaca aaaagagcaa ccaaagggac
     9061 aacctcggct ggttaccata tttactaaag gttccccttc tacttgggtc atccaatatt
     9121 tagaaagagg caagagtctt cgctgaggga aggggatttc atccagattt tggggaagag
     9181 gagcaggagg ggtacggaca atttttcctt cttcccggaa agaaatacct gaaacagatc
     9241 gaggagattc gttgttttcc agaactttaa ctagctggga gaaagtgtat tctccttctc
     9301 cccgcactat gaaatctatt aaaccagtat ttaataactc ttcatcacag aaggtagcgt
     9361 gatatccccc caatactacc agtcgtccct cttttttagc tgattcagct attttcaaag
     9421 cctcaggggc tttgttagtt tcgcatgtta ttcctaccaa atcacaattt tgtaaattct
     9481 tctgcagttc tttttttggt tctacttgaa ggtcgacgat ttttatccgg tgatgttctt
     9541 gctccaatgt acttgctaaa taagctaacc ctagaggggg taattttaag ccgatccagt
     9601 tataaacccc tggagatgga gggttaacca aaagaatgtt caataatctt cacctctttg
     9661 gcttctttca agaaagattt tttagttttt cgatgatagc ctctcctact tccttagttc
     9721 cacaagcagt gggatcatcg ggcgtgggtt tgaggtcata agtaaccttt tttccttctc
     9781 ttataacttc ttttattgct atttctaatt tttcagcttt ttctttttct cctatatatt
     9841 ttaacatcat catccctgag agaatcatag ctaaggggtt tactttattc attccccgat
     9901 atttaggagc actgccgtga gtaggctcaa ataatgcata ttcatcacca atgttagccc
     9961 ctggagctac tcccaatcct cctattagtc ctgctcccag gtcggatatt atatctccgt
    10021 aaaggttggg taaaaccaaa acgtcataga gctctggttt ctgcaccaat tgcatgctca
    10081 tattatctac tatacggtcc tcaaattcta tatcagggta atttttagct acctctcgag
    10141 ctacagccaa gaataaaccg tcagagtatt tcataatgtt agctttatgg acggcggtaa
    10201 ctttttttcg attttcagaa cgagcatatt cgaaagcaaa ttttacaatt ctttctgaac
    10261 caaaaacgga aataggtttg atgctgatac ctgaatcagg acggatttta ccttttcccc
    10321 atttttctat tgcttcgatg agctttagag tttcgggttt acctttctcg aattctatac
    10381 cagcatataa gtcttcagta ttttcccgaa tgattactaa atcaatattc tgataaagag
    10441 agcggactcc gggataagtt ttgcaaggac gaacacaggc gtaaaggtct aactcttttc
    10501 gtaaagctac attaacgcta cgaaacccac ttcctaccgg agtagtgata ggtcctttca
    10561 aagctacttt atttttcttt atagattcaa tcactgaggg aggaagagga gtaccatatt
    10621 cttccattac ttctgctcct gctttctgaa tatcccactc tatttctact cctgtagctt
    10681 caatcacttt tttggttaca gcagtaattt ctggcccaac accatctccg ggtattaaag
    10741 ttatacgata gggcattttt tctcttcctt tctctaagaa tttgcttcga taatttattc
    10801 tatcacaaaa tccaagcgag gaaagtcatc ctgattttta acaggaaagc acctacccta
    10861 ttcgactcct gtatatggta ggttaattat agtatcccgc aaaactg
//
LOCUS       DAOSVE010000059        10752 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_3425_length_10752_cov_67.898570, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000059 DAOSVE010000000
VERSION     DAOSVE010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 10752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10752
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3425_length_10752_cov_67.898570"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..545)
                     /locus_tag="PK016_05925"
     CDS             complement(15..545)
                     /locus_tag="PK016_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HXL02641.1"
                     /translation="MKRTKALVYLGIFTTLSIILTRLASIRIPIGGVEVIRIGFGQLP
                     VIMAGIYFGPGAGAVVGGLSDFLGFFLNPMGPYLPHFTLTAILHGLLPGLIFHHSSQP
                     LRKRVFWSITIPQVIVGIFLTSYFLHSIFGIPWNILLLPRLVSAPIQIFIYFYLLLLL
                     SRAPILSLTSFPNSHL"
     gene            complement(573..1496)
                     /locus_tag="PK016_05930"
     CDS             complement(573..1496)
                     /locus_tag="PK016_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL02642.1"
                     /translation="MEIGTLFNLSILQATIRMATPLTFAALGGMFCERAGVINIGLEG
                     IMLNSAFWAVFAAYFSGSSGVGFLAGVLSGMALGAVLAIFTVKYYADQIVIGTGINIF
                     GLGFSAFMCQKIWGSRGASGAVAGLPSLSIPLLNKIPYLGPLLNNHSILVYLSFLAFI
                     FSHYLLFHTPWGLRLRAVGENPQSADTAGINVHLYKIVFVILGSTLAGLGGVFLSLAH
                     LNFFAWGMTNGRGFIALAAMIFGKWTPVGCFLASLLFGFADALQMRIQTLGILPPQII
                     LLLPYLITILVLAGLVGRATPPSDYKPYIKK"
     gene            complement(1499..2545)
                     /locus_tag="PK016_05935"
     CDS             complement(1499..2545)
                     /locus_tag="PK016_05935"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL02643.1"
                     /translation="MRFSLKKSPSHFSILPEIIAILLAFFIGALILLFSGYSPLVAFS
                     ALFRGAFGSRNAWGQTLTQATPILFTALAFLLTFRAGIFNIGVEGQFLMGAMASALAG
                     IYFSGLPAPLHIALSLFLGGAMGALWGLIPALLKVYLGAHEVITTMMMSYIASYLTSY
                     MVNYPFKAPGWVAQTEPVAKTALLPKILPPTQLSLSFIISLFLSLLVSFFLFHTYLGF
                     QIRAVGLNPGVAENRGIEIKNRQILAFLLSGFIAGIGGGGEILGVHGRFVDGFSPGYG
                     WDGIAVSLVGKLHPGGCVLASILFGMLRAGGMTMTRVTKVPLDLSLIIQSLVIILVSA
                     PYFIKVVILRKKDF"
     gene            complement(2529..4052)
                     /locus_tag="PK016_05940"
     CDS             complement(2529..4052)
                     /locus_tag="PK016_05940"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL02644.1"
                     /translation="MPKILQTIGLTKSFPGVLANDHIDLEIEEGEIHGLLGENGAGKS
                     VLMSTIYGLYRPDEGKILIRGKEVTIDSPHTAIQYGIGMVHQNLTLVSSFRVWENIVL
                     GKEIKKGLSLNEKETLKKLEELLKMANLEVDLRAKTEELFLGSQQRIEILKILYRGAE
                     ILIFDEPTTILTPQEVDQLINTFRSLKKEGKTIIFISHKLKEIFAVCDRVTVLRKGRK
                     VGTLPIEEAAPEKLAEMMVGRDVLFSFPKGKKPGEEIILKLEGIATPVEKGKVALQNI
                     NFNLHVGEILGIAGVEGNGQRELAELIVGLIKPRKGRIFFRGEDITFLSTKERIKRGI
                     AHIPEDRMQRGIISDFTVWENLIIGFEDQPPFVKGKFTLNHSFLKQFSSQKIKEFEID
                     APGNDVLVKNLSGGNQQRVVLARELSGNVVLLVASQPTRGLDIAATEYLRNKLLELKN
                     QDKGIILISADLDEIRSLSDRVLVLYEGKIMGELSPESDEYQFGLLMGGKSIDEVLA"
     gene            complement(4054..5076)
                     /locus_tag="PK016_05945"
     CDS             complement(4054..5076)
                     /locus_tag="PK016_05945"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HXL02645.1"
                     /translation="MKKGRFLVLTVMIFVLLLPSISWGEGKKIGLILATGGLGDKSFN
                     DISYAGAVRAKEELGIELDYVEPKAVAEYEGYQRDFASSQEYEIIVCIGFDQADALNK
                     IAQEFPQQKFALVDMTVDQPNVASLLFKANEGGFLLGVQAAYMSKTKKIGIVGGMDIP
                     LIRDFFIGFEAGVEWANTGAEVLPTVYVGAWADPTKGKELALSLIDQGCDVIWAAAGK
                     SGLGALEAAKERGVYAMGVDACQCYLGDHIIASETKRVDVAVYETIKSAFDGTFQGGI
                     LEKGVAEGWIGNCRFPEEQPFWEETFNFKHQVEIPPEVMQKVEEAKEKISKGEIKVPR
                     PEEFGG"
     assembly_gap    5320..5419
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5826..6521)
                     /locus_tag="PK016_05950"
     CDS             complement(5826..6521)
                     /locus_tag="PK016_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HXL02646.1"
                     /translation="MLEELKQKVFEANMLLPKNNLVVLTWGNVSEIDRKSNLVVIKPS
                     GVSYEKLRPEMMVVVNEKGEVVEGNLKPSVDTPTHLELYRQFKDVGGIAHTHSTWATI
                     WAQAGMNIPCLGGTHADHFAREIPCTRVLTKEEVENDMELNTGKVIVETFKNLNYHEI
                     SAVLEAFHGPFTWGKDSLEAVQNSIVLEEVAKMSYFTLKLNPHASMPEEIIKVRYLRK
                     HGAKAYYGQNVTL"
     gene            7490..8290
                     /locus_tag="PK016_05955"
     CDS             7490..8290
                     /locus_tag="PK016_05955"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL02647.1"
                     /translation="MDLGIEGKVALVTGSSQGMGKAIARELSREGAQVILSSRSSAKL
                     EEAQKEIEKETGKKCLFFPCDLSNKEDIKNLVGYIAKEVKGVKGVDILVINSGGPPPG
                     NIFSITEEDWYKAFDLLLMSAIRLTKAFLPYMEAKNWGRIIYLTSTSIKEPVENLLLS
                     NVLRSGVNALSKSLSRDYAHRNILFNVVCPGSIATDRAIELLKEKSARSGQPLELLKQ
                     EAEKAIPMKRYGNPEEVASLVTFLCSNQATYLTGVAIQVDGGAIHFVL"
     gene            8750..10627
                     /locus_tag="PK016_05960"
     CDS             8750..10627
                     /locus_tag="PK016_05960"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXL02648.1"
                     /translation="MTLKVKVLSSFIVMIVLVTIVGYIGFLATSRLNDSLYKVYTVRM
                     PALDYLIEADRDLQQLLVAERSMLSVPKDSEIFKRFIADYEENFNQSIERFTKYKELA
                     KAPEELQLISAYEEARKEWETLSKQVIDYLTKGSEEEHNLAIELSLGKAGEKFDETRE
                     YLNQLTDVILSLTEEENLNAQKSRAMSLSLITWTIIISIIAGLAFALLITGSIRKPLQ
                     EISNAAEKIASGDLRVKVKKLTVKDELGKLSNSFAGMVNTLESLVHNTLNSSSLLASS
                     SQELASSVSEASRATTEIAKTISQVAEGSIRQSEELQKTSEEGNSIAKKAESIVNTSD
                     NNLHLLEEIIKKLEENSHTLSTVSKMNDSVNEQSEIAQEKAQNGKKLLIDLVASVENT
                     AQVSQEVAQSVELLTERSQEIGKIVDLITGIAEQTNLLALNAAIEAARAGDAGRGFAV
                     VAEEVRKLAEESAQAAEQIGSLISEIQKNTSETSEAMQRAKTQVEMGSKQSEKVYQSF
                     EDIISATSMVTDSIKGLTRTIEELEANQQATTENTQTLVKHTEDSLYDLRSISESINK
                     LNETITTIASIAEENAASSEEISASVEEQNAAMEEINSMAASLSKLAEELQKAVNSFR
                     V"
BASE COUNT         3511 a         2201 c         1969 g         2971 t
ORIGIN      
        1 ataatagacg ctctctataa gtgagaattg gggaaagaag taagtgataa tataggagcc
       61 cgagacaaaa gaagtaaaag ataaaagtaa atgaatatct gaataggagc gctaaccaga
      121 cgagggagaa gaagaatatt ccagggtatt ccaaaaatgg agtgcagaaa ataagaggtt
      181 agaaaaatcc ctacaatcac ttgaggaata gttatagacc agaacactct cttcctgaga
      241 ggttgagaac tatggtgaaa tatgagtccg ggaagtagtc catgcaaaat agcagtgaga
      301 gtaaagtgag gtagataagg tcccatgggg ttcaagaaaa accctaaaaa atcagataaa
      361 cctcctacca cagctccggc ccccggtcca aaatatattc cagccattat tactggtagc
      421 tggccaaaac caattcgaat tacctccact cctcctatgg gaatacgaat actggctaaa
      481 cgagttaaaa taatgcttaa agtggtaaaa atccctaaat aaactaaagc tttcgttctc
      541 ttcacctctg ttgcgttcct cctatcctta atctactttt taatataggg cttataatca
      601 gaaggaggag tagctctccc tactaaacca gctaaaacca ggatagttat gagataagga
      661 agaagtaaaa taatctgggg gggtaatatc cccagggtct gaattctcat ctggagagca
      721 tcagcaaatc caaagaggag actggccaag aaacatccga caggagtcca tttaccaaaa
      781 atcatagctg ccagagcaat aaatcctcta ccattggtca ttccccaggc aaaaaagttg
      841 agatgggcca gtgacagaaa tactcctccc aaccccgcca gggtagaacc taaaataaca
      901 aaaactattt tgtagaggtg tacgttaatc cctgctgtat cagctgattg agggttttct
      961 cctaccgctc gtaatctcaa accccaagga gtatgaaata agagataatg agagaaaata
     1021 aaagctaaaa aagagagata aaccaggata ctatggttgt ttaaaagtgg cccgagataa
     1081 ggaattttat tcagcaaagg gatagaaaga gaaggaagtc ctgcaaccgc tcctgatgct
     1141 cctcgagaac cccagatttt ctgacacata aaggcactga agcctaaacc aaaaatattt
     1201 attcctgttc ctataacaat ctggtcagca taatatttta cagtaaaaat agctaatacc
     1261 gctcccaatg ccatccccga tagtactcca gctaaaaacc ctacacctga gctaccggaa
     1321 aaataggcgg caaacactgc ccagaaggca gaattaagca taatgccttc taaaccaata
     1381 tttatgaccc ctgctctttc acaaaacatt ccccccagag cagcaaaagt gagaggagtg
     1441 gccatacgaa tagtagcttg taaaatactg agattaaaaa gagttcctat ctccattttt
     1501 agaaatcctt tttcctcaaa ataactactt ttataaaata aggagcagaa acaaggatta
     1561 taaccagaga ttgaatgatt aaagaaagat ctagcggtac tttggtaact cgagtcatag
     1621 tcatgcctcc tgctcggagc atcccgaaaa gaatagaagc taaaacgcag cccccgggat
     1681 gaagtttgcc taccagacta actgcgattc catcccatcc atatccagga gaaaatccat
     1741 ccacgaaacg gccatgcact cccagtattt ctccgccacc tcctataccg gcaataaaac
     1801 cactcagaag gaaagccaat atttgacggt ttttgatttc tataccccgg ttttctgcta
     1861 ctccagggtt gagtcccact gctcgaattt gaaagcccaa ataagtatga aaaaggaaaa
     1921 aagataccaa aagagataga aataaggaga taatgaaaga aagagaaagc tgagtaggag
     1981 gaagaatttt aggaaggaga gcagttttgg ccacaggttc agtttgagcc acccaaccag
     2041 gagctttaaa aggatagttc accatgtaac tggtaagata agaagcaata tagctcatca
     2101 tcatagtagt aataacctca tgcgctccta aataaacttt taatagagct ggaattaatc
     2161 cccacaatgc tcccattgct cctcccagga aaagagaaag agcaatatgc agtggcgcag
     2221 gaagtccgga gaaatatatt ccagccaaag ctgaagccat agctcccata agaaattgtc
     2281 cctctacccc aatattgaag attccggccc ggaaagtgag aagaaaagct aaagcggtaa
     2341 aaagaatggg ggtagcctga gtgagagttt gcccccaggc atttctacta ccgaaggctc
     2401 ctcgaaaaag agcagaaaaa gcaaccaggg gtgagtagcc agaaaataat agaatcagag
     2461 caccgataaa aaaagccaat aaaatagcta taatttctgg cagaatggaa aaatgagaag
     2521 gagatttctt aagcgagaac ctcatctata cttttccctc ccataagaag accgaactga
     2581 tattcatcac tttctggaga aagttcaccc ataattttac cctcatatag taccaggact
     2641 ctatcgctta aagagcgaat ttcatcaagg tcggcagata ttaaaattat tcctttgtcc
     2701 tggtttttga gttccaacaa cttatttctc aggtattcag tagcagctat atctaaaccc
     2761 ctcgtaggtt gagaagctac aagaagaacc acattccctg atagttcacg ggctaaaacc
     2821 actctctgct ggtttcctcc gcttaaattt tttactaata catcgttccc tggagcatct
     2881 atttcaaatt ctttaatctt ttgagaggaa aactgcttca aaaaagaatg gttgagagta
     2941 aattttccct ttacgaaggg aggctgatct tcaaaaccaa ttatcaaatt ttcccacacg
     3001 gtaaaatcac ttattattcc tctttgcata cggtcttcag ggatatgagc aatacctcgc
     3061 ttaattctct ctttagtaga aagaaaggtg atatcttctc cccggaaaaa gattcttccc
     3121 tttcgaggtt taattaaacc tacaatcagc tcagctaatt ctctctgacc atttccctcc
     3181 actccagcga tgcctaaaat ttccccgaca tgaagattaa aattgatatt ctgtaaagca
     3241 actttccctt tttccaccgg agtagctatg ccctccagct tcaagatgat ttcttctcct
     3301 ggttttttcc ctttagggaa ggaaaataat acatcccttc ccaccatcat ttctgctaat
     3361 ttttcgggag cagcttcttc aatgggcaaa gttcccactt ttctcccttt tctcaaaaca
     3421 gtaactctat cgcagactgc aaatatttct ttcaacttgt gagaaataaa aattatagtt
     3481 tttccctctt ttttaaggct tcgaaaggta ttgattagct ggtctacttc ttgaggggta
     3541 agaatagtag tgggctcatc aaaaataaga atttctgccc ctcgataaag gattttcaga
     3601 atttctatcc tctgttgaga acctaagaaa agctcttcag tttttgctct caaatctact
     3661 tccaggttag ccatttttaa aagctcttct agctttttaa gggtctcttt ttcatttaaa
     3721 gacaggcctt ttttaatttc tttccccaga acaatatttt cccatactcg aaaagaagag
     3781 acgagagtta aattctgatg taccattcca ataccgtact ggatagcagt atggggagaa
     3841 tcaatcgtta cttctttccc tcgtatcagt attttcccct catcggggcg gtaaagcccg
     3901 tagatagtgc tcataagaac tgactttccc gccccgtttt cccctaatag tccatggatt
     3961 tctccctctt ctatttctaa atcaatgtga tcattagcta aaactcctgg aaaactttta
     4021 gtgagaccaa tagtttgtaa tattttgggc atttcaaccg ccaaattctt ccggacgagg
     4081 gacttttatt tctcctttac tgattttttc tttagcttcc tcgacttttt gcatcacttc
     4141 aggaggtatt tctacttgat gcttaaaatt aaaagtttct tcccagaaag gttgttcttc
     4201 agggaaacga caattaccaa tccacccttc agctactcct ttttccagaa ttcctccctg
     4261 gaaagtgcca tcaaaagcag atttaatggt ttcatataca gcaacatcta ctcttttggt
     4321 ttcactggca ataatatggt ctcccaggta gcactggcaa gcatctaccc ccatagcata
     4381 tacgcctctt tcttttgccg cttccagtgc tcctaatcca ctttttcctg ctgccgccca
     4441 tattacgtca caaccctggt caataaggct gagagctaat tcttttcctt tagtggggtc
     4501 agcccaggct cccacatata cagtgggaag aacttctgct ccagtattag cccattctac
     4561 ccctgcctca aaccctataa agaagtctct aattaaagga atatccattc ctcctactat
     4621 gccaatcttt tttgttttgc tcatataggc agcctgtact cccaggagaa aacctccctc
     4681 attagcctta aaaagaaggg aggctacatt gggctggtct actgtcatat ctaccagagc
     4741 aaatttctgt tgaggaaact cctgagcgat cttattcaga gcgtcagcct ggtcaaatcc
     4801 tatacaaacg ataatttcat actcctgcga agaggcaaaa tcacgctgat aaccctcata
     4861 ttcagctacg gctttaggtt ccacataatc gagctctatt cctaattcct ctttggctcg
     4921 taccgctccg gcataggaaa tatcgttaaa ggatttatct cctaatcctc cagtagccag
     4981 gataagcccg attttctttc cctctcccca ggaaatggag ggaagaagta aaacgaaaat
     5041 cataacggtt agcaccaaaa aacgcccttt tttcattttt tcctcactcc tttttaagtt
     5101 ttaagaattt taataccagc acaaatcatt gtaatctgga aaaatgggaa aagcaagaag
     5161 cattaagtgt aaaagttttt atagtgttcc acaaaacttg ttggtaattg ctatcccttg
     5221 cggagaagaa aaaagcctgt ccttatgacc tcttcttttt cgtcattact gaacgtctct
     5281 atcaggaatc caaactataa gggggttacc cccctatcan nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnna ttactgaacg tctctatcag gaatccaaac tataaggggg
     5461 ttacccccct atcagcctct tttctttttc tcatcacccc ggcaatttta agccgttttt
     5521 aacttgcccc tgaatagtta gttctgcgag gggtctaaag tataggggag aaaaacccct
     5581 atcaccctct ttgaaaacaa caaacataat aaagaacaaa gactactgga agaggaaaaa
     5641 cgtctctcca gtacgagaga aaagaacatt attatggatt ttgcagttaa acataacagg
     5701 ttttgtggga tactttagtt ttaaaaagga tgacgctctc tattagcatc taaaacacaa
     5761 aaaaatcccc tccttgaaat ttataaaagg ttagaagtta tatctaaagc agactggaaa
     5821 caaatttata aagttacgtt ctggccataa taagctttag ctccatgttt ccgtaagtaa
     5881 cgaactttta taatttcctc aggcatagaa gcgtgaggat taagctttag tgtgaaataa
     5941 ctcattttcg ccacttcttc cagaacaata ctattttgca ccgcctccag gctatccttg
     6001 ccccaggtaa aaggaccgtg aaaagcttct aaaacggcag aaatttcatg gtagttaaga
     6061 tttttaaaag tttctactat tactttcccg gtatttaact ccatgtcatt ttctacctct
     6121 tctttagtta atacccgggt acaggggatt tctctagcaa aatggtcagc atgagtccct
     6181 cccaaacaag gtatgttcat ccctgcttga gcccatatag tagcccaggt agagtgagta
     6241 tgtgctattc caccaacatc tttaaactgg cgatagagtt ctaagtgggt gggagtgtcc
     6301 actgagggct tgaggtttcc ttctactact tctcctttct catttaccac taccatcatt
     6361 tccggcctta gtttttcata agaaacacca ctaggcttga ttaccactaa atttgatttt
     6421 cggtcaattt cactaacgtt tccccaagtt aagaccacta agttgttttt aggcaaaagc
     6481 atgttggctt caaaaacttt ttgttttaac tcttctaaca ttttttcctc ctcatcaaat
     6541 ttaatatatg cttattttag ggaaaaaagc cttccatttc aatttttaag aaggagaaat
     6601 attttcctat tctgagtaac tcagacaata agagagcaga acacatgaat aataggaaaa
     6661 aagctatgtt gttttcttag tacaaagtta ttctttacct gagcagctac atgcaagatg
     6721 ctccctcctc tacctaaaag aggcagagta aaaagacaaa ttcagaatga aagaataatt
     6781 caagaagtac agaaacaaaa cagcttagga ttcacaattt tgtactttgg actactaatg
     6841 gatttattat atagacagct ttttctccag gagcctattt caagatgccg ggatttagca
     6901 ctactctttt ttggtgttac actcatttta gcagttaaac gcgtaagttc tggccttttt
     6961 acttttacca gtaaatggaa ctcgaaacga atgctatggt catcaatcat tgctgctttt
     7021 gtgtttttag tcgttgattt cttgtggttg ggtagcaagt ctatcgcaga gttaattata
     7081 agtagcgtta ccttctttat tgcattttac ggaatcaatt tgttaatgca atgtttttcc
     7141 agaaaaaaga atgacgaaat gctcaaagaa aactgaacaa actctaataa gaagttaaat
     7201 tacacaaatt atagttaacc gcaaaatcca ttgtaacctt tctttacccc acactgcttg
     7261 attttcctta tattgtgcct ctgagatgtc aattacgaat tcgccacgga aaagatgata
     7321 actcttatga cctttctttt tctcccttac ccggatagca atttagcaga tttttgggga
     7381 taacataaaa atacccctgt agtaaaataa atcagtggct ggatctcata gcatttatca
     7441 ccatattaag aaatgataca atttagtaaa tttatgagga gaaaaaaaga tggatttggg
     7501 gatagaaggt aaagtagctt tagtaaccgg aagtagtcaa ggaatgggta aagccattgc
     7561 ccgggagtta tcccgagaag gagctcaggt tattctttct tcccgctctt cagctaaatt
     7621 agaagaagca caaaaagaga ttgaaaaaga aacgggcaag aaatgtttat tttttccctg
     7681 tgatttgagt aataaagaag acattaaaaa cctggtaggt tatatcgcaa aagaagtaaa
     7741 aggagtaaaa ggagtagata ttttagtaat aaatagtggt ggaccacccc caggaaatat
     7801 tttctctatc actgaggaag attggtataa agcttttgat ctgctcctta tgagtgctat
     7861 ccggttaact aaagcttttc tcccctatat ggaggctaaa aattggggga gaataattta
     7921 tctgacctcc acttctatca aagaaccggt ggaaaacctt ttgctttcta atgtcttgag
     7981 aagtggggtt aatgctttat ccaaaagtct ctcacgagat tatgctcatc gtaatattct
     8041 ctttaatgta gtttgccccg ggtctattgc cactgataga gcaatagagt tattaaaaga
     8101 aaaatctgct cgtagcggtc aacctttaga actcctaaaa caggaagctg agaaagccat
     8161 tcctatgaaa agatatggaa atccagaaga ggtagcttct ctagttacat ttttatgctc
     8221 taaccaggct acctacttaa ccggcgtggc tattcaggtt gatggaggag ctatccattt
     8281 cgtcttgtaa gtcagaagca aaacgccaga aagttccatc tttcctgact ataatgacct
     8341 tgaaagagat tgccacgccc ggcaaaaacc tcttcttatg tccagaccag atagatatgg
     8401 gtaatgctaa actcttaacc ccccaaaaaa aagcaaaaga aaagaacaaa gacttaaaag
     8461 gcaaataaac aaaaactact ggtgggaaaa tatccaccag tacgctgaga aaaaagcagt
     8521 ctgcgacgca agattaggaa aacttaaaaa acaaaagaaa taaaggccat agagaaagac
     8581 aaagtctttc tctatgcata gcgaccttga gtttttgact ttcggtgtaa tttacttcct
     8641 aaagttttta atattttaac cagttttgcg ggatatatac ctaaacgcta aaatttttcc
     8701 tgaaaatgcc gatgtataaa gtagaatata aacaagagag gagaaaaaaa tgactctaaa
     8761 agttaaagtt ttaagtagct ttatagtaat gatagttctg gtaaccattg ttggatacat
     8821 tggttttctt gctacttcca ggttgaacga ctctctctat aaagtataca cagtgcgaat
     8881 gcctgctctt gactacctca tcgaggcaga tagggatttg cagcaacttt tagtagctga
     8941 acgctctatg ctctcagttc ctaaagatag tgaaatattc aagcgtttta tcgctgatta
     9001 tgaagaaaat tttaatcaat caatagagcg ctttaccaag tacaaagaat tagccaaggc
     9061 accggaagaa ttgcaactta tctccgcata cgaagaagct cgtaaagaat gggaaacgtt
     9121 atcgaagcag gtgattgact atcttactaa gggcagcgaa gaagaacata atctggcaat
     9181 cgagttatct ttaggaaaag ctggtgaaaa atttgatgaa acacgtgaat atttaaatca
     9241 actcactgat gtcatattaa gcctgaccga agaagaaaac ctcaacgctc aaaagtctcg
     9301 tgctatgtcg ctatcgctaa taacctggac tattatcata agtattattg caggcttagc
     9361 atttgcttta ctgattaccg gaagcattcg taagcctctt caggaaattt ccaacgcagc
     9421 ggaaaaaatc gccagcggtg acttacgggt aaaagttaaa aaattaaccg taaaagacga
     9481 actgggcaaa ctgagcaata gctttgcagg aatggtaaat actttagaga gtttagtaca
     9541 taatacttta aattcctctt cccttttagc atcttcttct caagaacttg catcatcagt
     9601 gtctgaagcc agtagagcta ctacagagat cgctaagact atttcccagg ttgctgaagg
     9661 ttccatccgt cagagtgaag agctccaaaa aaccagcgaa gaaggaaata gtatcgccaa
     9721 gaaagctgaa agtatcgtaa acaccagtga taacaattta caccttcttg aagaaatcat
     9781 aaagaaatta gaagaaaaca gccatactct ttctactgtc agtaaaatga acgattcagt
     9841 caatgaacaa agtgaaatcg ctcaagaaaa agcccaaaat ggcaaaaaat tacttatcga
     9901 cctcgtagcc agtgtagaaa atactgccca ggtctcccag gaagttgccc aaagtgtgga
     9961 gcttttaacc gaacgttctc aagaaattgg gaaaattgta gatttaataa ccggaattgc
    10021 tgagcaaact aatctcttag ctttaaatgc agcaattgaa gccgctcgag caggagacgc
    10081 tggacggggg tttgccgtag tggctgagga ggtcagaaaa cttgctgagg aatcagctca
    10141 agccgcagaa caaattggaa gtttgatatc tgaaattcag aaaaatacca gtgaaacctc
    10201 tgaagcaatg cagagagcaa aaactcaagt agaaatgggt agtaaacaaa gtgagaaagt
    10261 atatcaaagc tttgaggata taatatcagc aaccagtatg gttactgact ctattaaagg
    10321 tctcaccaga accattgaag agttagaagc aaatcaacaa gcaactacgg agaacactca
    10381 gactctggta aaacatactg aggatagctt atacgatcta cgaagcatat ctgaatctat
    10441 taataaatta aatgagacca taactactat tgcttccatt gctgaggaga atgctgcttc
    10501 tagtgaggaa atttctgctt cagtagaaga acaaaatgca gctatggaag aaattaattc
    10561 tatggctgcc agcttgagca aattggctga agaactgcaa aaagctgtta atagcttcag
    10621 agtataaaca aagatagaaa acatatagtt aaccgcaaaa tccattataa tggctataag
    10681 ggggcttacc cccttatagc cccccaaaag gcaaaaaagt taaaaacaac tgagggagaa
    10741 agagtctcct tc
//
LOCUS       DAOSVE010000060        10668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_3458_length_10668_cov_77.425610, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000060 DAOSVE010000000
VERSION     DAOSVE010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 10668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10668
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3458_length_10668_cov_77.425610"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..77)
                     /locus_tag="PK016_05965"
     tRNA            complement(3..77)
                     /locus_tag="PK016_05965"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43..45),aa:Val,seq:tac)
     gene            complement(117..1205)
                     /gene="serC"
                     /locus_tag="PK016_05970"
     CDS             complement(117..1205)
                     /gene="serC"
                     /locus_tag="PK016_05970"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HXL02649.1"
                     /translation="MSEKRVFVFNPGPAALPLPVLQHVQEEMLNFRGTGMSIMEVSHR
                     SREFEEVLNSAVERIKRLLGLNEHYYVLFLQGGASLQFAMVPMNCVKNGKPVAYVDTG
                     YWATKAIKEAQNIVDNVEVVASSKDKNYTYIPKDILLEKDLSYLHITANNTIKGTQWW
                     RFPETGDVPLAADMSSDIFSRVFDPHPFGLIYAGAQKNAGPAGVTIVIIREDMLERVP
                     QNLPTMLKYSTFAESNSLYNTPPCFAVYVIELVAQWLEETIGGIAKMEEINRKKAHLL
                     YDYIDSQDFYKNPVQPEDRSWMNVIFNLPSPELESSFLSEATREGLIGLKGHRSVGGC
                     RASLYNAVSLEAVEVLVSFMKEFARRNG"
     gene            complement(1833..2216)
                     /locus_tag="PK016_05975"
     CDS             complement(1833..2216)
                     /locus_tag="PK016_05975"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXL02650.1"
                     /translation="MKFQGLHHAAIKTNNLGKTLDFYCGILGLEIEERFYDAGEQSEI
                     VFLSLGNTRLEVIAPHRLEEKDIVFSPYFHLALATSNVEETYEELRKKGINFTLPLTE
                     SGSFKYAYFSGPMGETLEIVERIKS"
     gene            complement(2227..4506)
                     /locus_tag="PK016_05980"
     CDS             complement(2227..4506)
                     /locus_tag="PK016_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HXL02651.1"
                     /translation="MKTVTKSVNDLLSRMTLEEKIAQLGSIHAHRLIEDGQFSRGKAR
                     ELLRNGIGQITRMAGIVDIRPEKAAELANEIQKFLKEETRLGIPAMIHEECLSGFTTW
                     GATVFPQAIGMGSTFDPDIIQNATSVIGKQMRALGAHQGLSPVLDIPRDPRWGRTEET
                     FGEDPYLVSRMASAYIKGLQGEDFPSGIIATAKHFPAYGISEGGRNLAPARVSERELR
                     EVFLFPFEVAVREAGVGSVMNAYHEIDGVPCAASRFFLTRILREEWGFEGLVVSDYGA
                     VRMLENFHRVAGSAKEAAVKALEAGIDVELPDADCYGEPLLEAVREGLISEEVINESV
                     RRVLEVKLLLGLREKESIEIDPQKVKESFDTPEDRELSREVARKSMVLLQNDGVLPLR
                     KDLGTIAVIGPNAQSTRNLHGDYSYTVHVPSVQKLKGEEVAGEDAVRTVSVLEGIKSK
                     VSANTKVLYAQGCDLLDTSCDGFEEALELAAQSEVIVAVMGENSGLFKNSISGEGSDR
                     VDLDLPGVQKELLQALKETGKPLVLVLVNGRPLSLNWEKENISAILETWYPGEEGGNA
                     IADVLFGDYNPGGKLPISFPQSVGQIPVYYNRKPSSFGEYISTDARALFPFGHGLSYT
                     EFSYSDLKITPPQVNSLEEVKVSFKVKNTGSREGDEVIQLYICDRVASVERPVKELKG
                     FKRLTLQPGEEREVMFTLFPEQLAFYDEFMRLIVEPGVFEVMIGSSSEDIRLSGEFEV
                     AKEKVLTKYRKFSSEVLIK"
     gene            complement(4594..5595)
                     /locus_tag="PK016_05985"
     CDS             complement(4594..5595)
                     /locus_tag="PK016_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL02652.1"
                     /translation="MNKKDNNPEKEELIRAKNLQEVFVIGGGFARSRLLAVDDVSFQV
                     NKNEIFTLAGESGCGKTTVSKILLGFLEPTQGEVYYKNKFIGDLRKWEEKKEFMKEVQ
                     SIFQNPFETFNPLRKVDSYLCDTAKYFGMMKGKGDKKAIEEALQSVGLGMEEVEGRYP
                     SEFSGGQLQRISIARALITKPSLLVADEPVSMVDASLRMSIVNLFKELRDNHGVSVLY
                     ITHDLATAYYVSDRIGIMFRGNIIEMGDVEEVLMNPKHPYTQMLKQSIPEADPKKKWI
                     ERVSITELETEEYMRKGCKFAGRCAFIMEKCKDHMPEEIFVDGRMVRCWLYGGEKKE"
     gene            complement(5592..6587)
                     /locus_tag="PK016_05990"
     CDS             complement(5592..6587)
                     /locus_tag="PK016_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL02653.1"
                     /translation="MDILKTENLKSYYILEMGTEKKEVKAVNDVTIGIHEDEIYGIAG
                     ESGCGKSTFLKTICKLVEPPLRMVGGSIYYAVDGKYVDVAEVKEEEFRKLRWKYISYI
                     PQGSMNALNPIIRVKESFKDFFTAHQKINDEKKDFEEPLKQHLSALGLPMRVLKSYPH
                     QLSGGMRQRTTIALATILKPRIIVADEPTTALDVVAQRAVIQLLKDIQKLQKNTIILV
                     THDMAVHANITDRIGIMYAGMFVEEAKTEEIFENPLHPYTRFLIASLPKMGDKSYKPS
                     VPGTPPSLSNLPPGCPFHPRCPYVMDICRKERPPLVETNTDHKSACFLSSKEGLQ"
     gene            complement(6591..7451)
                     /locus_tag="PK016_05995"
     CDS             complement(6591..7451)
                     /locus_tag="PK016_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL02654.1"
                     /translation="MESFKELMKDGRFKFAFIVICVLAFMSLLSFFSPYNPLKWNVVP
                     RDLPPRWPHIFGTTSQGQDLFWQSTFAIRNSLTIALIASSISRVIAIIVGLVAGYRGG
                     RTDTILMSINDSFVVMPLLPILILIASVMRGNLTMVALGLILSLFGWAWDARVIRSQV
                     LSLREREFTYTAILSGTKTFNLVIKEYFPFIIPIIFSTIINNMIWAIGMEVTLAILGL
                     TNIEVPTIGTMIHWAVNYQSMLLGYWWWILTPVVISIFLFVALYLLSSSLSEYLDPRT
                     RIQRIGEARG"
     gene            complement(7462..8469)
                     /locus_tag="PK016_06000"
     CDS             complement(7462..8469)
                     /locus_tag="PK016_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL02655.1"
                     /translation="MLFFKRYLLPRLVQFLVVVFVGITIVFIVPRLSPADPVQEVINR
                     ITSQGAFLDPAAVDNMRKSLTELYGLGGNVFQQYFAFWGRLFRGDFGPSLFQFPTPVT
                     QLIMNSLPWTAGLLIATTLISWIVGNFLGGIAGYFKNRRWAKILDNVCMVIRPIPYYI
                     FALLLLILFAYLFPVFPSAGGYSVGRRIGFNWAFISDVLKHSFLPAISLMVLGMAANF
                     MTMKLIVSNIVAEDYVMYAKAGGLKESKIAFKYVFKNGLLPQITGLALSLGQIFGGAL
                     ITEIVFSYPGIGTLIYGAINSGDYNLIMGVTSLSVIGIAGATLIIDLLYPLFDPRIRH
                     R"
     gene            complement(8531..10375)
                     /locus_tag="PK016_06005"
     CDS             complement(8531..10375)
                     /locus_tag="PK016_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL02656.1"
                     /translation="MGFAGISFAQLSEDTPREETLIANVLTGRVGTPGNFNVFTTAWR
                     NPDRGVQQLMLEPLWIVDPTTGEVINALAREAPIYNEDFTQMTVKLREGCQWSDGAPI
                     TADDIVYGVELCMQNSGMSYNAPFSLYVDKVYKTDDYTVVFELKESNSRFHTNFVDRW
                     GGWRPFPKHIFEKLEDPLTFDFNPPVSSGPYVLKDFDQAGYWTLWERREDWDKTPTGM
                     LYGMPKPKYVLFYYYGEATNQVIAQANHNLDMTDLTMEAFRAILERNEYSRGYRKEYP
                     WVVNVDPCMTGITFNNDVPPFNLRDVRWALTLAIDIVDYIGIAFDGAATMGVLHVPPI
                     PAYQEWYYKPMEEWLKNFTLDIEVNGEPFKPYDPDAPFRLVEYAKSRGYTVPEDPEEI
                     REAFGPGWWKYAPDVAEQLLLRNGFTRDADGKWLLPDGTPWKITITAHPSPTHPQHRN
                     SLAAAQQWRNFGIDVTVSPSEAYASLAQTGDYVVSDNWPAIEPWGGHFDLSRVLSPFR
                     TSYYRPIGEQAVGHSSRWYNEELDKVIEEMEKIDWDDPKNLELGMEGLKILVREMPTI
                     PTFSYPGVVAWDEYYWTNYPGAENPYTQPYHHWPNFKYMLPFLEPVQR"
BASE COUNT         2961 a         2512 c         1962 g         3233 t
ORIGIN      
        1 tctggtgggc gaaggaggac ttgaacctcc gaccccttgc ttgtaaggca agtgctcttc
       61 ctctgagcta ttcgcccaca ctaaaaatta tatcataaaa tgcttccaaa aactatttat
      121 ccgtttcgcc gagcaaattc cttcataaaa ctcaccagca cctctactgc ctcgagagaa
      181 acagcgttat aaagagaggc acggcatcct cccacggagc ggtgaccttt aaggccaatc
      241 aacccttccc gggtggcttc actaaggaag ctactttcta attctggaga agggagatta
      301 aatattacat tcatccaaga gcggtcctcg ggctgcaccg gatttttata aaaatcctgg
      361 ctatctatat agtcataaag cagatgagct tttttacgat ttatctcttc cattttagca
      421 atgccaccta tggtttcttc cagccactgg gcaactagct ctatcacgta aactgcgaaa
      481 caaggaggag tgttataaag ggagttactt tctgcaaaag tagagtattt cagcatggta
      541 ggtaaattct gtggaactcg ctccagcata tcttcccgaa taataactat ggtcactcct
      601 gccggaccgg catttttttg tgccccagca taaataagcc caaaagggtg agggtcaaaa
      661 actctgctga agatgtccga tgacatatca gcagccagag gaacatctcc cgtttctgga
      721 aatctccacc attgagtacc ttttatggta ttgttagcag taatatgaag ataagaaaga
      781 tctttctcca gcaatatgtc tttaggaata taagtatagt ttttatcttt ggaagaggcc
      841 actacctcta cattatctac aatattctgt gcttccttaa ttgcttttgt tgcccagtaa
      901 ccagtatcca cataagcaac gggtttccca ttctttacac aattcatagg aaccatagca
      961 aattgcaagc ttgctcctcc ctgtagaaaa agcacataat aatgctcatt aaggcccaga
     1021 agtcttttta ttctctctac agcactgttt aaaacttctt caaactcacg agagcggtgg
     1081 ctcacctcca taatagacat ccctgttccc cggaagttca gcatctcttc ctgaacatgc
     1141 tggagaacag gaagcggtaa agctgctggc ccagggttaa aaacaaaaac tcttttctcg
     1201 ctcataagct cctcaccttc taagtttata gttagtttta ttttcaggcc tttgcccggt
     1261 tataaatatc tttaattgta acagaatttc ttctatctga taagtaatca aatcattttt
     1321 taacttcatc atgtactacc ttcatttttc actatagtac ggggctgctt gaaaattatt
     1381 ctcctttata cctatcgtcg caactctata cctcgttttt gccatcgtga ggagcaaaca
     1441 gccatggtac tctcccacag gatcacgagg gtgacaaaaa acagcctatg gcgtttcccc
     1501 tctccttcat cacgaggagc aaagtgacga agcgatcccg cttttcaaaa acgaggtcgc
     1561 tacaggaagc gagcagacga agctcgtagc gatggttcaa taggaattgt cacataatta
     1621 ttccttgctg tacagtagag aagattgagc attaaacagg aaaaatattc ttagggcttt
     1681 tgcagttaac tataaagtaa aaaggaatac ctccatctct tgatatatca atattttgaa
     1741 attataacta tcaaagtcga ggatagaagg gaaatcccag aaaatagaaa gaggaggctc
     1801 tcctccagca agaaagagga gagcctcctc ttttagcttt ttattctttc cactatctcc
     1861 agagtttctc ccatcggtcc agaaaaatag gcatacttga agcttccgct ttctgtgaga
     1921 gggagagtga agtttattcc ttttttcctc aactcctcat aagtttcttc tacattagaa
     1981 gtagccagtg ccagatggaa ataaggagag aagacaatat ccttctcttc cagccgatgg
     2041 ggggcaataa cttctaaacg ggtgtttcct aaagagagga aaacaatctc tgattgctct
     2101 cctgcatcat aaaatcgctc ctctatctcc aaccctaaaa taccacaata aaaatccaga
     2161 gtcttaccca ggttattagt tttaatggct gcatggtgta aaccttgaaa tttcataagc
     2221 ttcctactac tttattaaaa cttcgctgct gaattttcta tacttggtaa gtactttttc
     2281 ttttgccacc tcaaattctc ctgaaagccg gatatcctct gaagaacttc ctatcataac
     2341 ttcaaaaact cctggctcta ctatgagcct cataaactca tcataaaaag ctaattgctc
     2401 gggaaaaagg gtgaacataa cctctctctc ttcaccaggc tggagagtga gacgcttaaa
     2461 ccctttgagt tcttttaccg gacgctctac tgaagctacc cgatcgcaaa tatacaactg
     2521 tattacctca tctccctccc ggcttccagt gtttttaact ttaaaactta ccttgacttc
     2581 ttctaaggag ttgacctgag gaggagtaat ttttaaatcc gagtaagaaa attcagtata
     2641 gcttaaacca tgtccaaagg ggaaaagagc tcgagcgtca gtgcttatat attcaccgaa
     2701 agaagaaggc ttccggttat aataaactgg aatctgtcct acactttgtg ggaaagagat
     2761 ggggagcttc cctcccgggt tataatcacc gaatagtaca tcggctatgg cgtttcctcc
     2821 ttcctctccg ggataccagg tttctaaaat ggcagagata ttttctttct cccagttaag
     2881 agaaagtggt cgaccattaa ctaaaactaa aactaggggt ttcccagttt ctttaagagc
     2941 ctgaaggagc tctttctgta ctccggggag gtctaaatct acccggtcac ttccctctcc
     3001 agaaatagaa ttcttaaata gaccactatt ttctcccata actgcaacaa taacctcaga
     3061 ttgtgctgcc agttccagtg cttcctcgaa accatcacag gatgtatcca gaaggtcgca
     3121 tccctgagca taaagaactt tagtgttcgc agaaactttg ctctttattc cttccagcac
     3181 actcaccgtt ctcaccgcat cttctccagc aacttcctct cccttcaatt tttggacgct
     3241 ggggacatgc acggtataac tataatcccc gtgcaagttt ctagtacttt gagcattagg
     3301 ccctataact gctatcgttc ccaggtcttt ccggaggggt aataccccat cgttttggag
     3361 aagaaccata gactttcgag ctacctcccg ggatagctcc cggtcttcag gagtgtcaaa
     3421 agattccttc actttttgag gatctatttc tatactttct ttttctctaa gtcccagaag
     3481 aagctttact tctaatactc ttctaactga ctcatttatc acttcttcag aaattaatcc
     3541 ttcccgtact gcttcaagta gtggttctcc ataacagtca gcatcaggaa gctcaacatc
     3601 tatccctgcc tctagggcct ttacagctgc ctctttagca ctaccagcta ctcgatggaa
     3661 gttttccaac attcttacag ctccataatc agaaaccacc aacccctcaa atccccattc
     3721 ttcacgcagg attctggtta aaaagaatcg agaggcagca caggggacac catctatctc
     3781 atgataggca ttcataaccg agcctacccc ggcttctctc actgccactt caaaggggaa
     3841 aagaaacact tctctcaatt ctcgttccga aacccgggca ggagccaggt ttcttccccc
     3901 ttcagaaatt ccataagcgg gaaaatgctt ggcagtggca atgatacctg atgggaaatc
     3961 ctctccctga agacctttta tataagcaga agccattcga gaaacgagat aagggtcttc
     4021 accaaaggtt tcctccgttc ttccccagcg gggatctcga gggatatcaa gaaccggaga
     4081 taatccctgg tgagcaccta aagctctcat ttgctttcct atcactgaag tggcattttg
     4141 aataatatcc gggtcaaaag tgctgcccat acctatagcc tgggggaata cggttgctcc
     4201 ccaggtagta aagccgctta ggcattcttc gtgaatcata gccggaatcc cgaggcgagt
     4261 ttcttccttc aaaaattttt gaatttcgtt agctagttca gccgcttttt ctggtcttat
     4321 atctactata ccggccattc tggtaatctg ccctatacca tttcttaaaa gttctcgggc
     4381 tttccctcga gaaaactggc cgtcttctat cagccgatgg gcatgaatag agcccagctg
     4441 agctattttt tcttccagag tcatgcgaga aagcaaatca ttaaccgatt tagttacagt
     4501 tttcataaag ctaacctcct attggtaatt tttgagtaaa atattatggc tatgtttctc
     4561 cagattaaaa ggcttttcat ttctataatg gttctattcc tttttctctc caccatacaa
     4621 ccagcatctt accattcttc catccacaaa aatttcctct ggcatgtggt ccttacattt
     4681 ctccattata aaagcacacc ttccggcaaa cttgcaccct tttctcatat actcctcagt
     4741 ttccagctcc gttatgctca ctctctctat ccattttttc ttgggatcag cctcaggtat
     4801 agattgtttt aacatctggg tataaggatg tttgggattc atcagcactt cctctacatc
     4861 acccatttct attatgttcc ctcgaaacat tattcctatt ctatcgctca cataataagc
     4921 ggttgcgagg tcatgagtta tgtataaaac actcacaccg tggttatctc tcagctcttt
     4981 aaagagatta actattgaca tcctcaatga ggcatccacc atggaaaccg gctcatcagc
     5041 tactaaaaga gatggtttag ttatcagcgc ccgggctatg gatatccttt gcagttgccc
     5101 tcccgaaaat tcactgggat acctcccctc tacctcttcc atgcccaatc ccactgattg
     5161 gagagcttct tctatagctt ttttatctcc tttccctttc atcattccaa agtattttgc
     5221 agtatcgcat aaataactat ctacttttct caaggggttg aaagtttcaa agggattctg
     5281 gaatatggat tgtacctctt tcataaattc ttttttctct tcccatttcc ggaggtctcc
     5341 gataaattta tttttatagt acacttcccc ttgagtgggc tcaagaaaac ctaaaagaat
     5401 ttttgatacc gtcgtctttc cacagccact ttctccagct aaggtaaata tttcgttttt
     5461 atttacctgg aaagatacat catcaactgc taagagtcta ctccgggcaa aaccccctcc
     5521 tattacgaat acctcttgca ggttttttgc tcttatcagt tcttcttttt ccggattatt
     5581 gtctttttta ttcattgtag cccctccttg gaggataaga aacaagctga tttatgatcg
     5641 gtattcgttt ctactaatgg gggtctttct tttctacaga tatccatgac atagggacac
     5701 cgggggtgga agggacatcc cggcggtaaa ttagaaagcg aaggtggagt acctggtaca
     5761 ctgggtttat aacttttatc tcccatctta ggaagagaag caattaaaaa cctggtatag
     5821 ggatgcaagg gattttcaaa tatctcttca gttttagctt cttctacaaa catgccagcg
     5881 tacattattc ctattctatc ggttatattg gcatgcactg ccatgtcatg ggtaactaaa
     5941 attatcgtgt ttttctgtaa tttttgaata tcttttaaga gctgtattac tgctctttga
     6001 gccactacat ccagggcagt ggtgggttca tcagctacta ttattctagg tttcaaaata
     6061 gttgctaaag caatggtagt tctctgtctc atccctcctg agagctgatg aggataagat
     6121 ttcagcaccc tcatcggtaa tcccagagct gataagtgtt gttttaacgg ttcttcaaaa
     6181 tctttctttt catcatttat cttctgatgg gcagtaaaga agtccttaaa tgactccttt
     6241 actcttatta tgggattcag agcgttcata gaaccctggg ggatataaga tatatatttc
     6301 caccgtagtt ttctgaactc ttcctctttc acttccgcta catctacata tttcccatct
     6361 actgcgtagt atatacttcc tcccaccatc cgcaggggtg gttctaccaa tttacatatg
     6421 gtttttaaaa atgtactttt tccacaacca ctttctccag ctattccgta tatctcatct
     6481 tcatgaatac cgatagttac atcatttact gcttttactt cttttttctc tgttcccatt
     6541 tccaagatat agtaagattt taaattttct gttttcagga tgtccatatt ttaacccctt
     6601 gcctccccga ttctctggat tctggttcga gggtcaagat actcactcag gctgctggag
     6661 agtaaatata aagccacaaa caggaatata gatatcacaa ccggcgttaa tatccaccac
     6721 cagtatccca gaagcatgga ctggtagtta actgcccagt gaatcatagt acctatggtg
     6781 ggtacttcta tgttggtcaa gcctaatatg gccagagtca cttccattcc tattgcccag
     6841 atcatgttat ttattattgt agaaaatatt atcggtatta tgaaagggaa gtactcttta
     6901 atcactaagt taaatgtttt tgtgcccgat aggatagcag tgtaagtaaa ttctctttct
     6961 ctcaagctca atacctgtga ccttataact ctggcatccc aggcccagcc aaataagctc
     7021 agtattaacc ccagagctac catggttaag ttccctctca tcactgaagc tatcagtata
     7081 aggatgggta aaagaggcat aactacaaaa ctatcgttta tggacatgag aatagtatcc
     7141 gttcttcctc cccggtatcc ggccaccaat cccactatta ttgctatcac tctcgatatc
     7201 gatgaagcta tcaaggctat ggtcagcgag tttcttatgg caaatgtaga ttgccagaag
     7261 aggtcttgtc cctgggaagt agtcccaaat atgtggggcc atcttggcgg caggtctcga
     7321 ggcactacat tccatttgag aggattgtaa ggagaaaaga aagagagcag agacataaaa
     7381 gcgaggacgc atatcactat gaaagcaaat ttgaatctcc catctttcat cagttcttta
     7441 aaagattcca aggcagtcac ctcatctatg tcttattcgt ggatcaaaga gggggtatag
     7501 gagatcgatt atcaacgttg ctcctgctat ccctataacc gagagagaag ttacacccat
     7561 tatcaggttg taatcaccag agttgatggc tccatatatc aatgtcccta ttccgggata
     7621 ggaaaatact atctcagtta tcaaagctcc cccaaatatc tgccctaatg atagggcaag
     7681 accagttatc tggggcaata acccattttt gaatacgtat ttgaaagcta ttttggattc
     7741 tttaagcccc ccggctttgg catacataac gtagtcctct gctactatgt ttgagactat
     7801 gagtttcata gtcataaagt tggctgccat tcccagaacc attaaagaga tagcagggag
     7861 aaaagaatgt ttcaagacat cggaaataaa agcccaatta aagcctattc tccttcccac
     7921 cgaatatcct cctgcagaag gaaaaactgg aaagagatag gcaaataata taagaagcaa
     7981 cagggcaaat atataatagg gtataggcct tatgaccata catacgttat ccagtatttt
     8041 tgcccatctt ctattcttga aatatcctgc tattcctcct aaaaaattac ccactatcca
     8101 ggaaatcaag gtagtagcaa tcaacaaccc tgctgtccag ggcagagaat tcattatgag
     8161 ctgagtaact ggagtgggga actggaacaa ggaaggacca aaatcacctc gaaacaacct
     8221 cccccagaaa gcaaaatatt gctgaaagac gttgcctcct aaaccataca gttcagttag
     8281 tgattttcgc atgttatcta ctgcagccgg gtctaaaaaa gccccctggg aagttattcg
     8341 attgataact tcctggaccg ggtcagcagg gcttagtctc ggtactatga aaactatggt
     8401 tatccctaca aagaccacta ctaaaaactg aactagtctt ggaagtaaat aacgtttgaa
     8461 gaataacact gattcacctc cgtatccaat aatgccccca cacagggggg cattattgag
     8521 tatagcatat ttatctctgt acaggttcta agaaaggaag catgtattta aagttcggcc
     8581 agtgatgata tggttgggtg taaggatttt cagctccagg atagttagtc cagtaatatt
     8641 catcccaggc taccactcct ggataactaa atgtggggat agtgggcatt tccctgacaa
     8701 ggatttttaa accttccatg cccaattcca gattcttcgg gtcatcccag tcgatttttt
     8761 ccatttcttc tataactttg tctaattctt cattatacca tctcgaagag tggccaactg
     8821 cctgctcacc aatgggtcta tagtaagaag ttcggaaagg actcaacacc cgggacaggt
     8881 caaaatgccc tccccagggc tctattgccg gccagttgtc gctaactaca taatcaccgg
     8941 tctgggctaa tgacgcatag gcttcggaag ggctcacagt cacatcgatc ccaaagtttc
     9001 tccactgctg tgccgcagca agagaattac gatgctgggg gtgagtaggg ctgggatgag
     9061 cggttatggt gattttccag ggtgtaccat caggcaggag ccattttcca tctgcatctc
     9121 tggtgaaacc attcctgagc aataattgtt ctgctacatc aggagcatat ttccaccatc
     9181 ccggtccaaa ggcttctctt atttcttcgg ggtcttcagg tacagtgtag cctctactct
     9241 ttgcatactc aacaagtcgg aagggggcat cggggtcata aggtttgaaa ggttctccat
     9301 tcacttcaat atctaaagtg aagttcttca accactcttc cataggttta tagtaccatt
     9361 cttgatacgc aggtattgga ggtacatgca gaacccccat agtagcagca ccatcaaaag
     9421 ctattccaat gtagtctact atgtctattg ctagagtcaa agcccatctg acatctctca
     9481 gattaaacgg gggtacatcg ttgttgaaag ttattcctgt catacagggg tctacgttaa
     9541 ccacccaggg atattctttt ctataacctc ttgaatattc gtttctctct aatattgcac
     9601 ggaatgcttc catagttaaa tcggtcatat ctaagttatg atttgcttga gctatcacct
     9661 gatttgttgc ttctccatag tagtagaaaa gcacatattt aggtttgggc ataccatata
     9721 gcattccagt aggagtttta tcccaatctt ctcttcgctc ccagagagtc cagtagcctg
     9781 cctggtcgaa atctttaagt acataagggc cactgcttac ggggggatta aaatcaaaag
     9841 ttagagggtc ttctaatttt tcaaaaatgt gtttggggaa aggtctccat cctccccatc
     9901 tatctacaaa attagtatgg aatcgagagt tggattcttt aagttcaaac actactgtat
     9961 aatcatcggt tttatatact ttatccacgt aaagactgaa tggagcatta taagacatcc
    10021 cagagttttg catacataac tctaccccat acactatatc atctgcagtt atgggagctc
    10081 catcgctcca ctggcaacct tctctcagct ttactgtcat ctgagtgaag tcttcattgt
    10141 aaataggagc ttctctagct agagcattta taacttctcc tgtcgtaggg tcaactatcc
    10201 agagaggctc tagcatcaac tgctgtactc ctctatctgg atttctccag gcagtggtga
    10261 aaacgttaaa gttccctggt gtccctaccc tacctgtcag tacattagca attagggttt
    10321 cttctctggg agtatcctca gatagctgag caaaacttat cccggcaaaa cccatcacca
    10381 gtactaacaa agatacaaac aagattaaag agcttcttct caccagctac acctccagtc
    10441 tgaaaaataa tatctttttg ctcttaaatc ctctaacttt tttcagttcc ttattaatca
    10501 ctcatttttt aattagagat tatcccggtt aggccctcca cagttaaagt acatctactt
    10561 tctggaagtc ttaagataat caattaccta ctccatcatc cgcttacctc tcaattaacc
    10621 tacagggtaa agtaatccct ttttcctcac ctccttttat tctctctg
//
LOCUS       DAOSVE010000061        10565 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_3512_length_10565_cov_74.046527, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000061 DAOSVE010000000
VERSION     DAOSVE010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 10565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10565
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3512_length_10565_cov_74.046527"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            367..441
                     /locus_tag="PK016_06010"
     tRNA            367..441
                     /locus_tag="PK016_06010"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:399..401,aa:Val,seq:cac)
     gene            complement(443..778)
                     /locus_tag="PK016_06015"
                     /pseudo
     CDS             complement(443..778)
                     /locus_tag="PK016_06015"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
     gene            974..1135
                     /locus_tag="PK016_06020"
     CDS             974..1135
                     /locus_tag="PK016_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02657.1"
                     /translation="MDYIITDIPKDQIIKIKHPPVGKFGRYLFNGQRLFQRLLLVKLH
                     TEWMKDETK"
     gene            1247..1600
                     /locus_tag="PK016_06025"
     CDS             1247..1600
                     /locus_tag="PK016_06025"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXL02658.1"
                     /translation="MIDHVCLVVNNLEESRKYYERILNMESRKSNTDSKTILLENKEV
                     HFFITERDFPKEYIKMQHISIRTESIEAKRQELVEKGITELTMGEFRQFRYHNYKWIE
                     WQDPNGIRLEYVELI"
     gene            complement(1688..6646)
                     /gene="rpoC"
                     /locus_tag="PK016_06030"
     CDS             complement(1688..6646)
                     /gene="rpoC"
                     /locus_tag="PK016_06030"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HXL02659.1"
                     /translation="MIDVNDLGAIQIRLASPEEIRKWSHGEVKKPETINYRTLKPERD
                     GLFCEKIFGPTRDWECYCGKYKRVRDKGVVCDRCGVEITRSSVRRERLGHIELAAPVS
                     HIWYFKSIPSKMALILGILPKNLEKVLYFASGRKREDCYKVVDPGSTDLEAGSTIRDI
                     EYKIYSKYDPNFKADPAYRVVEVHSLPFTIGDKLSVKEYTRYRSKYKESFTVEEVEEG
                     QFEVVDIRVFPYQREDELSESEVKELQEIFGGLFEETPLSETVEEPCYMVIDPGQTGL
                     KVAQIILETEARLLSHYDSTFKAGVGAEAIKEILSNIDVNQLARELRKELREASGQKA
                     KKLIKRLQVVESFAKSGNRPEWMILETIPVLPPDLRPMVQLDGGRFATSDLNDLYRRV
                     INRNNRLKRLLQLGAPEIIVKNEKRMLQEAVDALFDNGRRGRPVLGPGNRPLKSLSDM
                     LKGKKGRFRQNLLGKRVDYSGRSVIVSGPRLAFDQCGVPKKMALELFKPFVMKKLVDR
                     GFAQNIKSAKRMVEKAREEEVWAALEEVIAEHPVLLNRAPTLHRLSIQAFQPVLVEGN
                     AIQLHPLACSAFNADFDGDQMAVHVPLSAEAQAESQIIMLSSHNVLSPASGRPVAVPT
                     QDMVLGCYYLTLSRGGESKKYFGDVNEALLAYDLGNLDLHEPIRIREITAKHPEIKET
                     TLGRAIFNELLPDGIPYYNEPITKKKLSEIADLCYRKLGNEATVAMLDNIKKRGFHFA
                     TRSGTTISVQDLEIPPEKWSIIDDANRRVEEINQHYAEGLITEEEREQKVIDIWTDAS
                     NKVADAILEDLDEFNPVNMMAKSGARGSIRQVSQLSGMRGLMADPSGRIIEYPIKANF
                     REGLSVLEYFISTHGARKGLADTALRTSKSGYLTRRLVDVAQDVIVREEDCGTEEGVL
                     LFDLRDENDHLIESFEERILGRVALKDIVDKNSGEILVHAGEEIDENVVSLFRERGIK
                     EALVRSPLTCRTRYGVCQKCYGRNLATGRLVDIGEAVGIITAQSIGEPGTQLTMRTFH
                     TGGIRVTGEDITQGLPRVEQLFEVRKPKKAAILSDVYGVVEKIETVGMRRKIYVIPDE
                     EEEDQSVRTYFVPQDIKICVTEGERVQAGDRLTLGPIDPRDILRVKGIKAVQEYLLNE
                     IQSVYISQGVSINDKHIEVIIRQIANLNKVMVENAGDTTLLSGELVFLEDFKEENEKI
                     GEENKLLIERAQDFLTDAVLMEEVLDITGEVILPRGKVLDEDDIRLILSSDCKSFLVE
                     KKSSLLRVVRGYRNLGVELADKVNVEEIKTSEGRVLLKAGELLKPEVLDQIAEMGPIK
                     LRARDKSIWEKLRGSILAEEVVDPQSGEVLLPASARLDNETIELLDEHGVEKVVVWKD
                     VKVFPIKESLVKALREEILGKEVAKDVISPQNGEVIVQAGQAISRGIARRLVSEGVKE
                     IPLSDGRFFSIEGRLIELLEKEIVGKVVAQDIVSGSDGVVMVKAGEIIDRDDVVKIAG
                     DNIGFLLIREPGEDKEIRSLVEEILYLPGLKQVATGRPVVLGITKASLAVDSFLSAAS
                     FQQTTHVLADAAIKGKVDELIGLKENVIIGKAIPAGTGFSKYRHIRLSTEGAEKEKKR
                     REELKEESLDMWELMGAGEAFEDDEEVEFNLGQDYFDDTGEG"
     gene            complement(6665..10399)
                     /gene="rpoB"
                     /locus_tag="PK016_06035"
     CDS             complement(6665..10399)
                     /gene="rpoB"
                     /locus_tag="PK016_06035"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HXL02660.1"
                     /translation="MSVSYIERQSFAKIPSSLELPDFIEIQKKSFDWFLQRDVPPESR
                     KKQGLQEVFLEVFPVQDFTGRLVLEFVNYRLEEPVFNVREAKEKGRTYSVPLHARIRL
                     IDRQTNAIKEQDVYMGDIPLMTEWGTFVVNGAERAVVTQLVRSPGVFFSREVASTGKV
                     DYGFKIIPNRGAWLEFGIDPGDIIYVRVDKRRKINAVTLVRALGYENNEEILSLFDYH
                     PVIRKTLERDPSTNYKEAVIEIFRKLRPSEPPTEENTRDFIKYLFFDPRHYDLGEVGR
                     YKITNKLKLEERVIRIEEPVLAEDIVIDLLNKECVRDINLLGEDIDFSRYRIVGRKGE
                     KINRRLAREVEELSEEYPIEYITVLNEEDLPVKIYVEREDTWEVLSEDLEGKVLSEPV
                     RDEVTGEVLVEKGRPLTLSLIRDLRGLGIERVRVKKGRTLTPEDVVGSIRYFLNLIDG
                     IGYEDDIDHLGNRRARMVGELLQNQFRIGLLRVEKVIRERMTIQPDLESATPQSLINV
                     RPIVAAMREFLGSSPLSQFMDQVNPLSEITHKRRLSALGPGGLSRERAGFEVRDVHYT
                     HYGRICPIETPEGPNIGLITSLCIFAKVNDYGFIETPYRRVVDGRVTDEVVYLSADEE
                     DLYHTAPPDVPLDEEGYIQAENIIARFREKIVEVPRDKIDFIDVSPQQIISASAALIP
                     FLEHNDANRALMGTNMQRQAVPLLVPHPPYIGTGIEYKVAQDSGATVIAKRNGVVKKA
                     DALQIEIEAEDGGVDVYELNKFQRSNQSTCINHKPIVRRGDRVEKGQIIADGPCTSMG
                     ELSLGRNVLVAFMPWEGENFEDAIMISERLVKEDVFTSIHIDEYEVEARDTKLGPEEI
                     TRDIPNLGEDMLRNLDKEGIVRIGSDVKPGDILVGKVTPKGETELTPEEKLLRAIFGE
                     KAREVRDTSLRIPHGEYGKVIDIKVFSRENGDELAPGVNKTVKLFVAQKRKISVGDKM
                     AGRHGNKGVVARIVREEDMPYLPDGTPVDIVLNPLGVPSRMNVGQVLETHLGWIANKE
                     RQFMSSPPFDGAREEEIMELMRKVEAPKEEGPFDYFFDSRVSPDFKLLPQGKTVLYDG
                     RTGEPFDNEVTVGYIYMLKLAHLVETKIHARSTGPYSLVTQQPLGGKAQFGGQRFGEM
                     EVWALEGYGAAYTLQEMLTVKSDDITGRVQAYEAIVKGKNILKPSLPESFKVLVKELQ
                     SLCLDVRVFDSKKREISLEEVEEEEEETPVLDINLQRREK"
BASE COUNT         3085 a         2497 c         1742 g         3241 t
ORIGIN      
        1 tgaagagact attatcagag aagaaataac ttttgaagaa aagacgcctt gctaaataaa
       61 aagtggatgg cttttgcaac aaagatgttt tatagatagg agaccatcca gttatgaaaa
      121 atactcctga tgaagcacaa aaacaagagt tgaatcaaat actaaaaagg agtttttaat
      181 gataactaaa gggaaattgg gagaaagtct cggtcgaata ggaattatcg aataaagagg
      241 ccaaaagcac aaaggagcta tctttagaaa tgtgatttat tacagaatag ccacaaacag
      301 tcttctgaca gctattgaag aatgtaatag cactatggta aaataatacg tagaaaaggt
      361 attagggcgc ggttaactca gcggtagagt gccatcctca cacggtggaa gtcgctggtt
      421 cgaatccagc accgcgcacc atttatttaa agtatcccac aaaacctgct aaagcgctta
      481 agacttgatt cctgtctatt atctctactg ctcttatctc atctaagctt tccctcactt
      541 tctctatggg tatatccagt cccttttctt ttaattcata ctgcatcact ctcagaatat
      601 agaagctcaa gaaacataac aggatgtgtc cttctattct tttaggagtc caatgatata
      661 ctggccggat atccaaagta cttttcataa tccggaaaga ttcttctatc ttccataagg
      721 tataataagc ttctaaggca tcttgaggtt tgatatcctc ttcgctgctc tctataccat
      781 agtaaccatc aagcttccca tctcaataga gggcttcttc atccacatac caggtttctt
      841 tattagtatt cttgagatat ttcttacctc ctcttttatt actggcagta attttagatt
      901 ggttctgtaa aagtttcgta gtaagaaacc cccccccgaa tgttataatt tatagaaaag
      961 ggaggaaaaa ttcgtggact atataattac tgatataccc aaagatcaaa ttataaaaat
     1021 aaaacaccca cctgtgggaa aatttggacg ctacctattt aatggacagc gtttatttca
     1081 aagactatta ttagtcaaat tacatacaga atggatgaag gatgaaacaa aataactgtg
     1141 aaaagattgt agtcgccgtt tcgtatggac atgactcagt acttgatttt tatcataaag
     1201 tggaatttta cgaaagactg atagaactgg aatataagga atagaaatga tcgatcatgt
     1261 atgcctggtt gtgaataatc ttgaagagtc acgaaagtat tatgaaagaa tcctgaatat
     1321 ggaaagcagg aaatcaaaca cggatagtaa aacaatattg cttgaaaata aagaagtgca
     1381 ttttttcata acagagcgag attttccaaa agaatatata aaaatgcagc atatatcaat
     1441 tcgaacagaa tcaatcgagg caaagaggca ggaattggtg gaaaaaggga taaccgaatt
     1501 aacaatggga gaattcagac aatttcgata tcacaattat aaatggattg aatggcagga
     1561 tccgaatgga atacggcttg aatatgttga attaatttag gaactgaact caacataaaa
     1621 taacctttcg aacctgagcc agggcaacaa gatacgaatt tttgaaaacc agataccggg
     1681 gaaagattta accttccccg gtatcgtcaa agtagtcttg gcctaaatta aattctactt
     1741 cttcatcgtc ctcaaatgct tctcctgctc ccattaactc ccacatatct aagctttctt
     1801 ctttcaattc ttctcttcgt tttttctctt tctctgctcc ttcagtagaa agacgaatat
     1861 gtctgtattt actaaagccg gtaccagcag gaatagcttt cccgatgatt acattttctt
     1921 taagaccaat tagttcatca actttacctt taatagctgc atcagctaaa acatgggtag
     1981 tctgctgaaa agaagcagca gaaagaaaac tatccactgc taaagaagcc ttggtaatgc
     2041 ccaaaaccac cggtcttcca gtagctacct gctttaatcc tggcaaatac aatatttcct
     2101 ccaccagact tctaatttct ttgtcttcac ctggttcccg gatgaggaga aatcctatat
     2161 tatctccagc aattttaacc acatcatctc tatcaattat ttctccagcc ttcaccatta
     2221 ctactccatc gctgccggat acaatatctt gagccaccac tttacccact atttcctttt
     2281 ctaaaagctc tatcaaccta ccttctatgg agaaaaatcg accatcagaa agagggattt
     2341 cttttacccc ttcactgact agtcgacgag ctatgcctct acttatcgcc tgtccagcct
     2401 gaacaataac ttcaccattt tgagggctaa ttacatcttt agctacttct ttccccagaa
     2461 tttcctcccg cagagcttta accagactct ctttaatggg aaaaacttta acgtccttcc
     2521 acactactac cttttcaact ccatgttcat caagtaattc aatagtttcg ttatccagtc
     2581 gagcgctagc tggaagaagt acttctccac tctgggggtc aactacttcc tcagccaata
     2641 ttgacccgcg gagtttttcc cagatagatt tgtctctggc tctcaattta attggtccca
     2701 tttccgctat ctggtccaga acctctggtt ttaacaattc accagctttt agtaaaactc
     2761 taccttctga agttttgatt tcttctacat taactttatc tgccagttct acccccagat
     2821 tacgataacc ccgtaccact cgaagtaaac tactcttttt ctccaccagg aaagacttac
     2881 aatcagaaga gagaatcagc cgaatatcat cttcgtccag gactttgcct cgaggtaata
     2941 taacttcacc ggttatatcc agtacttctt ccattaaaac ggcatcagtc aggaaatctt
     3001 gtgctctttc tattaaaagt ttattttctt ctccaatttt ttcattttcc tctttgaaat
     3061 cttccagaaa caccagttcc ccggagagaa gagtagtatc accagcattc tccaccataa
     3121 ctttgttgag attagcaatc tggcgaataa taacttcgat gtgtttatca ttaatgctta
     3181 ctccttgaga gatataaacc gattgaatct cgttgagaag gtattcctgc accgctttaa
     3241 tacctttaac tctcaatata tctcgagggt caataggtcc taaagtaagc cggtcaccag
     3301 cttgtactct ttctccctca gtaacgcaaa tctttatatc ttgaggaaca aaataagtac
     3361 gcactgattg gtcttcttct tcctcatcag ggataacgta tatttttctc ctcattccta
     3421 ctgtttctat tttctccact actccatata catcagaaag aatagcagct tttttaggtt
     3481 ttctcacctc aaatagctgt tccacccgag gcaaaccctg agtgatatct tcaccagtaa
     3541 ctcgaatacc accagtatgg aaagtacgca tggtgagctg agttcctggc tccccaattg
     3601 attgagcagt tattatcccc accgcctctc ctatatctac caatcgtcca gtagctaaat
     3661 ttctaccata gcatttctga cacactccgt agcgagttcg gcaagtaaga ggcgacctta
     3721 ccagagcttc ttttattcct ctttctctaa acagagaaac tacgttttca tcaatttctt
     3781 ctccagcatg aaccagtatt tctccgctat ttttatctac tatatccttt agtgctactc
     3841 ttcctaaaat tctttcttca aaagattcta taagatggtc attttcatct cgcaggtcaa
     3901 aaagtaaaac cccttcttcc gttccacaat cttcttctct aacaataaca tcttgagcaa
     3961 cgtctaccag gcggcgagtg aggtagccag atttagaagt acgcaaggca gtgtcagcta
     4021 ggccttttct tgctccatga gtagaaataa aatactccaa aacgcttaac ccttcccgga
     4081 aattagcttt tataggatat tctataattc ttcctgaagg atcagccatc aaacctcgca
     4141 taccagataa ctggctaacc tggcggatac ttcctcgagc ccctgattta gccatcatat
     4201 taaccgggtt gaattcatcc agatcttcga gaatagcatc ggctactttg ttagaagcgt
     4261 cagtccagat atctataact ttttgttctc tctcttcttc agtgattaga ccctcggcgt
     4321 agtgctggtt gatttcctcc actcggcggt tagcatcatc aatgatgctc catttctcag
     4381 gaggaatttc caggtcttgc acactaatgg ttgttccaga acgggtagca aaatgaaaac
     4441 ctctcttttt gatattatcc aacatggcta ccgtagcttc attacccagc tttcgataac
     4501 agaggtcagc aatttcagat agcttctttt tagttattgg ctcattgtaa taaggtatac
     4561 catcgggcag taactcatta aatatggctc taccaagagt ggtttctttt atttccggat
     4621 gttttgcagt aatttctcta attctgattg gttcgtgcaa atccaagtta cccaggtcat
     4681 aagcaagaag agcttcattt acatctccaa aatatttttt gctctctcca cctcgcgaca
     4741 gggtcaaata gtagcaaccc agtaccatat cctgggtggg aacagcaacc ggtctaccac
     4801 tggctggaga gagaacatta tgagaagaaa gcatgataat ttgtgactca gcctgtgcct
     4861 cagcagaaag aggaacatgt accgccatct gatctccatc aaagtcagca ttaaaagccg
     4921 aacaagctaa aggatgcagc tgaattgcgt taccctccac aagaacaggc tgaaaggcct
     4981 gaatgctcaa tcggtgaaga gtaggagccc ggttgagtaa aaccggatgt tcagctatca
     5041 cttcttctaa agccgcccat acttcttctt ctcgcgcttt ctctaccatc cttttggcac
     5101 ttttaatatt ctgagcaaaa cctctatcca ccagcttttt cataacaaaa ggtttaaaaa
     5161 gctccagagc cattttctta ggaacaccgc actggtcaaa agccagacga ggaccagaaa
     5221 caataaccga ccttcctgaa taatctactc ttttacccag aagattttgt ctgaaccttc
     5281 cctttttacc ttttaacata tcactaagtg atttcaaagg acgattcccc ggtccgagaa
     5341 ccggtctacc tcttctacca ttatcaaaaa gagcatcaac tgcttcctga agcattcttt
     5401 tttcattttt cacaatgatt tccggagcac ccagctggag aagcctcttt aagcggttat
     5461 tccggttaat tactcgtcga taaaggtcat tcaagtctga agtagcaaac cttcctccat
     5521 ctaactgtac catgggacgt aaatcaggag gcagaacagg aatagtctca agaatcatcc
     5581 attccggacg gttacccgat ttagcaaaag actctactac ctgtaatctc ttaatgagtt
     5641 ttttagcttt ctggccagaa gcctctctaa gttccttacg caattctcga gctaactgat
     5701 ttacatcaat attgctcaaa atttctttta ttgcttccgc tcccactcct gctttgaacg
     5761 tggagtcata atggctaagc aaccgagcct cagtttccaa gattatttgt gccaccttca
     5821 aacctgtttg tcccggatcg attaccatat aacaaggctc ttccactgtt tcagatagag
     5881 gagtctcttc aaagagcccc ccaaatattt cttgcaactc ttttacttca gattcagaaa
     5941 gttcatcttc ccgctgataa ggaaatactc gaatatcgac tacttcaaat tgaccttctt
     6001 ctacttcctc tacagtaaaa ctttctttat acttagaacg ataacgagta tattctttaa
     6061 cgctcaactt gtctccaata gtaaaaggaa gactgtggac ttctacaacc cggtaagcag
     6121 ggtcggcttt aaaattgggg tcatatttag aatagatttt atactctata tcccggatag
     6181 tggatcctgc ttccaaatca gtagaacccg gatccactac tttatagcaa tcttcccttt
     6241 tcctacctga ggcaaaatat aatacctttt ctagattttt aggtaaaatc cctaagataa
     6301 gagccatctt gctgggaata cttttgaaat accagatgtg ggagaccgga gcagctaatt
     6361 cgatgtgccc taatctctct cttctaactg aagagcgagt tatttccact ccgcagcggt
     6421 cacaaactac tcctttatct ctaactcttt tatattttcc acaatagcat tcccaatctc
     6481 gagtaggtcc aaatattttc tcacagaaaa gtccatctct ttctggtttc aaagtccgat
     6541 agtttatagt ctcgggcttt ttgacttctc cgtgagacca cttacgaatt tcttctggag
     6601 aagctaatct aatttggata gcacctaaat cattaacgtc tatcaaggat tctcctcctc
     6661 tattctattt ttctcttctc tggagattga tatctaaaac aggagtttct tcttcttcct
     6721 cttctacttc ctccagagaa atttctcttt ttttagaatc aaacactctc acatcaaggc
     6781 acaagctctg caattctttc accagtacct tgaaagactc aggaagagag ggtttaagaa
     6841 tattcttacc tttaacaata gcttcatagg cctgcactct ccctgtaatg tcatcagact
     6901 tcacggtaag catttcctgc aaagtataag ctgctccata tccttccaga gcccatacct
     6961 ccatttcacc aaatctctga ccaccaaact gagcttttcc tcccagaggt tgttgagtca
     7021 ccaaagagta agggccggta gaacgagcat gaattttagt ttctacaaga tgggctagtt
     7081 tgagcatata tatataaccc acggtaactt cgttatcaaa aggctctcct gttcttccat
     7141 cataaagaac agttttaccc tgagggagaa gcttgaaatc aggagaaact cgtgaatcaa
     7201 aaaagtaatc aaaggggcct tcctctttcg gagcttcaac ttttctcatg agttccataa
     7261 tttcttcttc tcgcgcccca tcaaagggag ggctggacat aaactgtctt tctttattgg
     7321 ctatccaccc taagtgagtt tccagaacct gccctacatt catccgggaa ggaactccca
     7381 gggggttaag cacaatatct actggtgttc catcggggag ataaggcata tcttcttctc
     7441 gaactattcg agctaccact cctttatttc catgtctacc agccatttta tctcctaccg
     7501 atattttcct cttttgagct acaaaaagtt ttaccgtctt gttcactccc ggagccaatt
     7561 catcaccatt ttcccgggag aaaactttaa tgtcgataac tttaccatac tctccatgag
     7621 gaatgcgaag tgatgtatct ctgacctctc gagctttttc tccaaatatt gcccgcaaaa
     7681 gtttctcctc cggagtaagt tcggtctctc ctttaggggt taccttgccc accagaatgt
     7741 cccccggttt aacatccgaa cctattctca ctatcccttc tttatccaag ttgcgcagca
     7801 tatcttctcc taaattcgga atatcccggg tgatttcctc cgggcctaat ttcgtatccc
     7861 gggcttccac ttcatattca tcgatatgaa tagaagtaaa aacgtcttct ttaaccaatc
     7921 tttcactgat cataatggcg tcttcaaaat tttctccttc ccagggcatg aaagccacaa
     7981 gaacgttcct acccagggat agttccccca tagaggtgca gggaccatca gcaatgattt
     8041 gccctttttc tactctgtct cctcttctaa ctattggttt atgattaata caggtactct
     8101 gattagacct ctggaattta ttcaattcat atacatccac tccgccatcc tctgcctcta
     8161 tttcaatttg tagagcatca gcttttttca ctactccatt tcttttagct ataactgtag
     8221 ctcctgaatc ttgcgccacc ttatattcta ttcccgtccc gatataggga ggatgaggaa
     8281 ctaaaagggg aacagcctgc ctttgcatgt tagtgcccat caacgcccga ttagcatcgt
     8341 tgtgctctaa aaagggaatc aaagcagcag aagcactaat aatctgctga ggagaaacat
     8401 caatgaaatc aattttatca cgaggaactt ccactatttt ttctctgaac cgggctatta
     8461 tgttctccgc ctggatataa ccttcttcat ccaagggcac gtcaggggga gcggtatgat
     8521 aaaggtcttc ttcatcagca gaaaggtaaa caacttcatc ggtaactctt ccatccacca
     8581 cccttcgata aggagtttct ataaatccat aatcattaac tttagcgaat atacacagag
     8641 aagtaataag accaatattg gggccttctg gagtttcaat aggacatatt cttccataat
     8701 gagtgtaatg aacgtctctt acttcaaaac cggccctttc ccggctcaaa cctccaggtc
     8761 ctaaagcgct taatcttcgc ttatgagtaa tctcagaaag aggattgacc tgatccatga
     8821 attgagaaag cgggctacta cccaaaaact ctcgcatagc agcaacaata ggcctcacgt
     8881 tgataagact ttgaggagta gctgattcca gatcaggctg aatagtcatt cgctcccgaa
     8941 tcactttctc tactcgtaaa agaccgatac gaaactgatt ctgtaaaagt tctcccacca
     9001 tacgagctct tcggttacca aggtggtcaa tatcatcttc ataaccaata ccatcgataa
     9061 ggttaagaaa gtagcgaata ctacctacta catcttcggg agtaagggtt cggccctttt
     9121 ttactctcac tctttctatt cccaatcctc gtaaatcccg gataagactt aaagttaaag
     9181 gtcttccctt ttctaccaaa acttctccgg taacctcatc tcgtaccggt tcactcaaaa
     9241 cctttccttc taaatcttca gaaagaacct cccaggtatc ttctcgttcc acataaattt
     9301 ttaccggcaa atcttcctca ttaagtactg ttatgtattc tattggatat tcttcgctaa
     9361 gctcttctac ttctcgagcc aagcgacggt taatcttctc tccttttctt cccactattc
     9421 gataacgaga aaaatcaatg tcttcgccca gtaaattgat atctcgtaca cactccttat
     9481 ttaacaaatc tataacaata tcttcagcta atactggttc ttctatccta ataactcttt
     9541 cttccaattt cagtttatta gttattttat atcgacctac ttcacccaaa tcataatggc
     9601 gagggtcaaa aaagagatat ttaataaaat ctcgggtatt ttcctcagta ggaggctcgc
     9661 taggacgaag tttacgaaag atttctatca ctgcctcttt atagttagta gaagggtctc
     9721 tctccagagt tttacggata accggatgat aatcaaatag agataaaatc tcttcgttat
     9781 tttcgtatcc taaagctcta actaaagtga cagcattaat ttttctcctt ttatccactc
     9841 gtacataaat gatatctcct ggatcgattc caaattctaa ccatgctccc cgattgggaa
     9901 taatcttaaa gccataatcc acttttccgg tagaagcaac ttctcgagag aaaaatactc
     9961 ccggagagcg aactaactga gtaaccactg ctcgttcagc accattaact acgaaggtgc
    10021 cccattcagt cataaggggt atatcaccca tgtacacatc ctgttctttt atcgcattag
    10081 tctgccggtc aattaatctt atccgtgcat gaagaggaac agaataagtt cttccttttt
    10141 ccttggcttc tctaacatta aaaactggct cctccaatcg ataattaaca aattccagaa
    10201 ccaaccttcc agtaaaatct tgaactggaa acacttccag aaaaacttcc tgcaatcctt
    10261 gctttttgcg gctttcaggt ggaacatctc tttgtaaaaa ccagtcaaag gacttctttt
    10321 gaatctctat aaaatcagga agttccaaag aagaaggaat tttggcaaaa ctctgccttt
    10381 caatataact tacactcact aatcacacca ccttttgagg gaaaatcgag tagcaatttt
    10441 tgttagatga taatttactt gaagaaagaa aaataacttt tcgtcctccc gtcttaaata
    10501 cacctgaaaa gacgaagaat cattatatag actttttagt caaatgtcaa caggtgtatc
    10561 actta
//
LOCUS       DAOSVE010000062        10429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_3563_length_10429_cov_73.306536, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000062 DAOSVE010000000
VERSION     DAOSVE010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 10429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10429
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3563_length_10429_cov_73.306536"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            440..1642
                     /gene="hisZ"
                     /locus_tag="PK016_06040"
     CDS             440..1642
                     /gene="hisZ"
                     /locus_tag="PK016_06040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HXL02661.1"
                     /translation="MELVLGMKDSYPREVKRKREMEFVLRHLFERWGYEEVEPPTIEY
                     YSTLENTLGDEAKERVYKFVDRNGKLVSLRPEFTTSIMRMYSKKELRESTPHRVYYVG
                     KIFRYPSSPLRIEGELTQLGLENLGKNGVDTDAEIIALSLSALLALQELGIEDFMLDI
                     GHVGIFQGVLSSLPLSHSEKLSLKTLLKKKDFVALENFTSISSHPLFPFLLHLPFLRG
                     KREILEETRKNYPLNSPLIDALDYLEEIWNIISDFHLEEHVFINLGLVRDFDYYTGVV
                     FEGFSPRSGYPLLAGGRYDELLEKSGNNLPACGFALFLERLMEVQKENLKESRLPFIT
                     INYPSRFRSSIFALAEKMRKEGIPVIMEEGKGEDIQVLQGKELIYQGKDEGKIKTLIQ
                     ERFKSWES"
     gene            1630..2271
                     /gene="hisG"
                     /locus_tag="PK016_06045"
     CDS             1630..2271
                     /gene="hisG"
                     /locus_tag="PK016_06045"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HXL02662.1"
                     /translation="MGKLTMSIPTGRMKEESLALLSRLPQCPPLDFVNSRQLVVERED
                     LPWRIIFSHPKDSSTYVEFGAADIGIVGKDILLERENEVYELLQLPIGKCTMVLAAPL
                     STSREELLEKEALRIATKYPNFTRRYLQESGITANVVFLYGSIELAPPIGLADAIVDL
                     VSTGKTLRENRLQVVEEIVPISGRLVANPVSIKTHSKEIYLLVRDLKEVMQHV"
     gene            2264..3562
                     /gene="hisD"
                     /locus_tag="PK016_06050"
     CDS             2264..3562
                     /gene="hisD"
                     /locus_tag="PK016_06050"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HXL02663.1"
                     /translation="MSEVPIVIKPVSNLEELSWSLQLNRQLLYKKLKKAEEEVKEIVE
                     RVMEEGDRALRYYTKKFDGCDIRKWLIEERELQNSWKEMDKSFQETIGEIEKNVRSFH
                     QKQKPFSWWEERSASFLGERWRPLNSVACYIPGGRFPYPSSLLMTVVPAQIAGVKEII
                     VLTPPDAQGKVKKEILATAFFLGVKKVYRVGGAQAIAGISFGTSSLPAVDKIVGPGNI
                     YVTAAKKLLQGIVGIDSLAGPSEVVIVADKTSSPHWLALDLLAQAEHDPLSLSILLSP
                     DEGILLETKRYLERELQNQPLSFSRETPIYLYQIESKEKVWEVVNFLAPEHLQIVTEN
                     PEKDCLKVKNAGALFLGPQAPVALGDYGYGPNHVLPTGRGAVFSSPLSVRDFGKFSSF
                     IYPQDDQTLQWEKFSRLAEIEGLYYHRRSLLARGGREWPL"
     gene            complement(3559..4407)
                     /gene="speE"
                     /locus_tag="PK016_06055"
     CDS             complement(3559..4407)
                     /gene="speE"
                     /locus_tag="PK016_06055"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HXL02664.1"
                     /translation="MELWYIQDYINEYKMGLKVKETIFQVKTSFQEITILDTYLYGQV
                     LLLDGIAQLSEKDEFMYHEMLVHVPMFSHPHPEKVLIIGGGDGGASREVLKHPVKELT
                     LVDIDEGVIEVCQKYFPHLGKWDDPRLTVLIEDARQYISSTSEVFDVIIMDSTDPLPQ
                     GVAEPLFTPEFFAQAFHRLSPQGIIVSQMEPPFFDADRVKKLCEGLKVFPIVNLYWGL
                     VPTYPGGIWSYVIASKSPDPRQQQRLPDFPTRYYSPGIHQAAFVLPPFLEDMLKNQKK
                     DKGNFS"
     gene            complement(4373..4762)
                     /gene="speD"
                     /locus_tag="PK016_06060"
     CDS             complement(4373..4762)
                     /gene="speD"
                     /locus_tag="PK016_06060"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HXL02665.1"
                     /translation="MNLEEGIVARHLLVEIIGSHSLNEKETMERFLQDLAQVYKSEIL
                     ALQVYQFQPFGLSALLVMPESHVAVHTWPEHGYAALDIFLKVDLRPQDSIPLIEQYFA
                     PQDIKIRELERGVKKKDGTLVHPGLHQ"
     gene            complement(4762..5502)
                     /gene="pgeF"
                     /locus_tag="PK016_06065"
     CDS             complement(4762..5502)
                     /gene="pgeF"
                     /locus_tag="PK016_06065"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HXL02666.1"
                     /translation="MKEKKVADYSFLQFENLSSFPSLEHFVTTRHPFLDLRKEEGRER
                     LRDILGGKDIFTVAQVHGVQWVVVGENSSPSSFLETQADALLTQRKGVYLGVKAADCL
                     PVIFFDPGREALALLHAGWRGIIQGIHLRVAQAMKNLFSSSYSQLVVGIGPGIGKCCF
                     AVGEEVVKLFEQKERGRENIDRKEGRFLINLKGFLFEELIDQGFDPRKIEVAPHCTFC
                     EKELFYSHRREGERAGRFMLLASLKEGR"
     gene            5800..6762
                     /locus_tag="PK016_06070"
     CDS             5800..6762
                     /locus_tag="PK016_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HXL02667.1"
                     /translation="MKIGVFLVLFQNEPLERALNYVKNIGVEAVEIGTGGYPGNAHLN
                     PEELLIKEDKIKKLKEAVESRGLEISGLSCHGNPVHPDKKIADKHHRDFKNTVLLAEK
                     LGVKNVITFSGCPGDSENSKYPNWVTCPWPNDFSEVVRWQWEEKLIPYWKKAGQFAQD
                     HGTRVAFEMHPGFCVYNPETLLKIRSAVGDVIGANFDPSHLFWQGIDPLLALRALEGA
                     VYHVHAKDTKIDPKNSLVNGNLDTKLYTRESERSWIFRTIGYGHDLGFWKDFVSTLRL
                     IGYDGVLSIEHEDSLMSGKEGFEKAVALLKEVVIKEKIGEAWWV"
     gene            6811..7164
                     /locus_tag="PK016_06075"
     CDS             6811..7164
                     /locus_tag="PK016_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HXL02668.1"
                     /translation="MAIDIKRVYNEAHESDGFRILVDRLWPRGLNKERANVDLWLKDI
                     APSEELRKWFSHDPAKGDEFKQRYFQGLDHKKELVSIVIQKAREGKVTLLYGAKEEKF
                     NNGMALKEYIEAKMR"
     gene            7240..8436
                     /locus_tag="PK016_06080"
     CDS             7240..8436
                     /locus_tag="PK016_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXL02669.1"
                     /translation="MSKSKEELYQERLKRYTKAMQNEKPDMIPIRPFVAEFTALYAGY
                     TCQEVTHDYEKAFLAARKCAADFDWDAVVGNMVYVWTGLTQAINLTYYATPGIDIPPD
                     VGFQYREPPEEKAFMKPEEYDDLIKDPTDYLFNVWLPRVSQDIVPPGKPTTFRHNLAF
                     LKGGMAMMQYFDALGRQNQLLREETGTVSAISGILKAPLDILADKLRGYYGLARDLME
                     RPDKVLAACEALMPHLFQVALSGADPEKNVPVAMWMHRTSVPFISPRHFQDIYWPTTK
                     PILEELWNKGHQTLLYAEGNWDFHLSTFRELPAGSVIFHVDRSDIFKAHRILGDRFCI
                     SGGIPNTLLSIGTPEEVRDYCKKVIDEVARDGGYIMDASAIIQNDAQVENVKALTDFT
                     REYGQY"
     gene            8447..8608
                     /locus_tag="PK016_06085"
     CDS             8447..8608
                     /locus_tag="PK016_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02670.1"
                     /translation="MEEVKRKPGACIPWEEKAKELPPLSGDPELVRRVWEEVDFLGYL
                     FIWHCLVSF"
     gene            complement(8605..9522)
                     /locus_tag="PK016_06090"
     CDS             complement(8605..9522)
                     /locus_tag="PK016_06090"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HXL02671.1"
                     /translation="MVNYIIAFNILFFLATILSGGFTLSNLLRWGAKFGPLVAHGEWQ
                     RLLICIFLHGNIWHLFFNLYALYYLGRMAEEIYGYRKFFGIYIISGVSSSILSYLLNY
                     SQVGVGASGAIFGLAGAIFASGLKYHNTSLHQIGMSLLPFIVINLLIGALSPAIDNAA
                     HLGGVLSGMLLGWVISPGSSWIRWKRTLEEVLYWATIAFVAFAILTFFVPNLAPGRAS
                     LDKIITFHNETQKILLKIETGFLPSPSEVEKLSPPDRKAREIKELLLTLLQEGGKDFS
                     LQREIEEKFFSWRDEILNKYQGIILEQSP"
     gene            complement(9522..10247)
                     /locus_tag="PK016_06095"
     CDS             complement(9522..10247)
                     /locus_tag="PK016_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HXL02672.1"
                     /translation="MVIFQFLEEKQVSMTVAILAVIFVILSVLLGVIGSLMLVTGRVV
                     GALGSWTVVALFGFAGAILQAVVLYQWSAAINQNLNNTRQLFSYLKEHIEDPLRGEIG
                     FFANRIEELIIPTWPYWIYLVTYFISLFTSWYALLCNLLGFIFLAIYMHNIFKSTSKV
                     SDLKNKVYDYLKEKKGATITGEVYRIPRRNILVFIILSVITLSIYWLYLLVKLSSEIN
                     NYVKSDEKMRKEIEEVFGATTQG"
BASE COUNT         3127 a         2024 c         2260 g         3018 t
ORIGIN      
        1 cttaaaaagc aaataaacaa aaactactgg tgggaaaata tccaccagta tgctgaagat
       61 aaaatactcc agcataacaa ccttgagttt ttgacttccg atgtaattta ctttttaaaa
      121 gtttgttagt ttaacaggtt ttgtgtaata ctataattat ccatatttct cctcgtcctt
      181 cgagggagag ggttagggtg agggtttttt cattatcatg gggatacctc tatcatccct
      241 tgctgtcatt ttttgaacgt attagagcgt aaaaagcaaa agactactga ggaaggtttt
      301 tcccctcagt aaatagagaa acactgttat gaaatttgca ggtaactata aaaatctact
      361 tttataatat acaatgtgat tctttgccgt tattttgcta aaatagttaa gatattattt
      421 aatgagagga gataatggtt tggaattagt cttaggtatg aaagattctt atccccggga
      481 ggtaaaaaga aaacgggaga tggaatttgt cctgcgccat ctttttgaga gatgggggta
      541 tgaggaagtg gagcctccca ctatagaata ttattctact ttagaaaata ccttaggaga
      601 tgaagccaag gaaagagtct ataaatttgt agaccgaaat ggtaagttag tctctctccg
      661 tcctgaattt accacttcca taatgcggat gtacagtaaa aaagagctga gagaatctac
      721 gcctcaccgg gtttactatg tagggaagat atttcgctat ccttcttcac ctctgcgaat
      781 agaaggtgag cttacccagc tgggcttaga aaacttaggt aaaaacggtg tagataccga
      841 tgccgaaata attgctctct ctctatcagc tcttttagct cttcaggaat tgggaataga
      901 agattttatg ctggatattg gtcacgttgg tatatttcag ggagtgttgt cttcccttcc
      961 tctttctcat tcagagaaac tttctctaaa aactttgctc aagaaaaagg attttgtggc
     1021 tctggaaaat tttacctcta tttcttctca ccctcttttc ccttttcttc tccatcttcc
     1081 tttcttgcga gggaaaagag aaatcttaga agaaactcgg aaaaattatc ctctcaattc
     1141 tcccttaatt gatgccttag attatctgga agaaatatgg aatatcatta gcgattttca
     1201 cctggaagaa catgtcttta ttaacttagg gttagtgaga gactttgatt actacaccgg
     1261 ggtagtattc gaaggatttt ctccccggtc aggatatcca cttttagcgg gaggaaggta
     1321 cgatgagctt ttagaaaagt caggaaataa cctgccggcc tgcggatttg ccttattttt
     1381 ggaacgcttg atggaagtgc aaaaagaaaa tcttaaagaa agccgccttc cttttattac
     1441 tataaattac ccttctcgtt ttcgttcctc tatctttgct ctggcagaaa aaatgagaaa
     1501 ggaaggaatc cccgtaataa tggaagaagg aaaaggagaa gatatccagg ttttacaagg
     1561 gaaagaactt atttatcagg gaaaggatga aggaaagatt aaaactctta tccaggagag
     1621 gttcaaatca tgggaaagct aactatgtcc atacctacag gtagaatgaa ggaggagtct
     1681 ttggctcttc tttctcgttt acctcagtgt ccacctcttg attttgttaa ctctcggcag
     1741 ctggtcgtag agcgagaaga cctgccctgg cgcattattt tttctcatcc caaagattcc
     1801 tctacttatg tagagtttgg agcggcggat ataggaatag tagggaagga tattctctta
     1861 gagagagaaa acgaagtcta tgaattatta caactcccta ttggaaaatg tactatggtt
     1921 ttagctgccc ctctatctac ttcccgggag gaactattag aaaaggaggc tctgcggatt
     1981 gccactaaat atcctaattt tacccgtcga tatcttcagg agagtggtat aactgctaat
     2041 gtagtttttc tttatggctc aattgaatta gctcctccca taggtttagc cgacgctatt
     2101 gtagatttag tctctacggg gaaaacttta cgagaaaacc gcttgcaggt agtggaagaa
     2161 atcgtcccta tctcgggtag attggtggct aaccccgtaa gtattaaaac tcattctaaa
     2221 gaaatttatc ttttagtaag agacttgaag gaagtgatgc agcatgtctg aagtgcccat
     2281 agtaatcaaa cctgtttcta acttagaaga gctatcgtgg agccttcagc tgaaccgtca
     2341 gctactctat aagaagctga aaaaagcaga ggaagaggta aaagaaattg tagagagagt
     2401 gatggaagaa ggagaccgag ctcttcgcta ctataccaaa aaatttgacg gttgcgatat
     2461 aagaaaatgg ctcatagaag aaagagagct ccaaaatagc tggaaagaaa tggataaaag
     2521 tttccaggag accattgggg aaatagagaa aaatgtgaga agttttcacc aaaaacagaa
     2581 gcctttttcc tggtgggagg agcgttctgc atctttcttg ggagaaaggt ggaggccact
     2641 gaattcggta gcctgctaca taccaggagg gagattcccc tatccttcat ctcttcttat
     2701 gactgtagtt cctgctcaaa tagcgggagt gaaagaaatc atagttctca ctcctcctga
     2761 tgctcagggg aaagtcaaaa aagagatttt agctaccgct ttctttctgg gggtgaaaaa
     2821 agtttaccgg gtaggagggg cacaggctat tgctggtatt tcttttggta cttcttctct
     2881 cccggcagtg gacaaaatag tggggccggg caatatttat gttactgcag ctaaaaaact
     2941 ccttcagggg atagtgggta ttgattcttt ggccggacca tcagaggtgg taattgtagc
     3001 cgataagacc tcttcacctc actggttggc actggacctt ttagctcagg cggaacacga
     3061 ccctctctct ttgagtatcc ttctctcacc cgatgaggga atactattgg aaaccaaaag
     3121 atatttagaa agggagttgc aaaaccagcc tctttctttc tctcgagaaa ctcccatata
     3181 tctatatcag atagaaagta aagaaaaagt ctgggaagta gttaatttcc ttgctcccga
     3241 gcacctgcag attgtaactg aaaacccgga aaaagattgt ttgaaagtaa aaaatgccgg
     3301 tgccctattt ttaggacctc aggcaccggt agctttaggc gattacggct atgggccaaa
     3361 ccatgtactt ccgacaggta gaggagcagt tttttcctct cccttatcag tgagggattt
     3421 tggtaagttc tcctctttta tttaccccca ggatgaccag acactacagt gggagaagtt
     3481 ttcccgatta gcagaaatag agggccttta ctaccatcga cgctcgttgc ttgctcgagg
     3541 aggcagagag tggccacttt agctaaaatt ccctttatct tttttctgat tttttagcat
     3601 atcctctaaa aaagggggta aaacaaaagc agcctggtga atgcccggtg aatagtaacg
     3661 ggtaggaaag tcagggagtc tttgctgctg tcgagggtcg ggagatttag aagcaattac
     3721 ataactccag attcctccgg gataggtagg caccaatccc cagtaaagat tcactatggg
     3781 aaaaactttc aagccttcac acagcttttt tactctatct gcatcaaaaa aaggaggttc
     3841 catctgagaa actattatcc cctgggggga aaggcggtgg aaagcctggg caaagaattc
     3901 gggggtgaag agaggctctg ccacgccctg ggggagaggg tcggtagaat ccataattat
     3961 gacatcaaaa acttctgaag tagaagaaat gtattgacgg gcatcttcaa ttaaaactgt
     4021 aagacggggg tcatcccact tccccaggtg aggaaaatac ttttgacaga cctcgataac
     4081 tccttcatcg atatccacga gagttagctc ttttaccgga tgctttagca cttcccgtga
     4141 tgctccccca tctcctcctc cgattatgag tactttttcc ggatgaggat gggaaaacat
     4201 gggaacatga accagcatct catgatacat gaattcatct ttttcactca actgggctat
     4261 accgtccagc agtaaaacct gcccgtagag ataggtatct aaaatagtaa tctcttgaaa
     4321 gctggtttta acctgaaaga tggtttcttt tactttaagc cccattttat attcattgat
     4381 gtaatcctgg atgtaccaga gttccatctt tctttttcac tcccctctct aattctctta
     4441 tttttatgtc ctgtggagca aaatattgct cgattaaagg aatactatcc tgaggtctca
     4501 aatccacttt gagaaagata tctaaagcgg catagccgtg ttcaggccag gtatggacag
     4561 ccacatgaga ttcaggcata accagaagtg cactcaaccc gaaaggctga aactgataga
     4621 cttgaagagc cagaatttca ctcttgtaga cctgggctag gtcttgtaaa aatctctcca
     4681 tggtttcttt ttcgtttaaa gaatgagagc caatgatttc taccaataaa tgacgggcca
     4741 caattccttc ttccaaattc atcaccttcc ttcttttaaa gaagcaagaa gcataaatct
     4801 tcctgctctt tccccttctc ttcgatgaga ataaaagagt tctttctcac aaaaggtgca
     4861 atgaggagcc acttctattt tgcgagggtc aaacccctgg tctatcaatt cctcgaagag
     4921 aaagcctttt aagtttatca aaaatctccc ttcttttcta tctatatttt cccttcctcg
     4981 ttccttttgc tcaaaaagtt ttaccacttc ttctcctact gcaaagcagc attttccaat
     5041 gcctggacct attcccacca ctagttgaga ataggaggaa gaaaagagat tcttcatagc
     5101 ttgagctacc cggaggtgaa tcccttgtat tattcctctc cacccggcat gtaaaagagc
     5161 cagagcctct cttcccgggt caaaaaaaat tactggaagg caatctgctg ctttcactcc
     5221 cagatatact ccttttctct gagtaaggag tgcatcagcc tgagtttcta aaaaagagga
     5281 gggggaagaa ttttctccca ccactaccca ctgcactcca tgcacttgag ctacagtgaa
     5341 gatatccttt ccccctaaaa tatctcttag cctctccctc ccttcttctt ttcttaagtc
     5401 aaggaaagga tggcgggtgg tgacaaagtg ctcaagagag ggaaaggaag aaagattttc
     5461 aaattgcaaa aaggagtaat cagctacttt cttttctttc atctccacct accatagaga
     5521 aaactcctgt ttaccaacta tatttaacca caaaaggggt aggaataaca cccctctggg
     5581 gtatttatta ggggagaaaa aaagatttaa gtagagggaa agaatagtaa cttatcgaag
     5641 ctgaacaaaa gaacaactcc gggatgacag aatggatgag ggtggggagc ttagtgtcat
     5701 tttctcctat ttaaatgctg gaaaacaaat accgtctttt ttcaataact tgtttatgat
     5761 atgctgaatc aaattttatt gaaaaagggg gaaaaaggaa tgaagatagg agtatttctg
     5821 gtgctttttc aaaacgagcc tctggaaaga gctcttaatt acgttaaaaa tataggagta
     5881 gaagcagtgg agattggcac cggaggatat ccaggtaatg ctcacttaaa cccggaagag
     5941 ctgcttatta aagaagacaa aataaaaaaa ctaaaagaag cggtagagtc ccgggggttg
     6001 gaaatcagcg gtttgagttg ccatggtaac ccggttcatc ccgacaaaaa gatagctgat
     6061 aagcatcatc gggatttcaa aaatacagtt cttttagctg aaaaactggg ggtaaaaaat
     6121 gtgattactt tctctggttg cccgggtgat tcggagaatt ctaaataccc taactgggtt
     6181 acctgtccct ggcccaatga tttttcagaa gtggttaggt ggcagtggga ggagaagcta
     6241 attccttatt ggaaaaaggc tggccaattt gctcaagacc atggaactcg ggtggctttt
     6301 gagatgcatc ctggtttctg tgtgtataat cctgagactt tattgaaaat ccgcagcgcg
     6361 gttggagatg taatcggagc taattttgat cccagccatc ttttctggca ggggatagac
     6421 cctcttttag ctctgagagc cttggaaggt gcagtttatc atgttcatgc taaagatacc
     6481 aaaattgatc ctaaaaatag tctagtaaat ggaaacttag atactaaact ctacactcgg
     6541 gaaagtgaga ggtcttggat atttcgaact attggttacg gacatgattt gggtttctgg
     6601 aaagattttg tgagtacctt gcgcctcatc ggctatgatg gagttttatc tatcgaacat
     6661 gaagattctc ttatgtccgg gaaagaaggg tttgaaaaag cagtggctct tttgaaagag
     6721 gtagtgatta aagaaaaaat tggagaagct tggtgggtat aatttttttg agagtgcctg
     6781 ttcattttta cgaatttagg gagttgaaga ttggccatag atattaagag agtttataat
     6841 gaagcccatg aaagcgatgg ttttagaata ttagtagata gactttggcc tcgaggtcta
     6901 aataaggaaa gggcaaatgt agacctatgg ttgaaagata ttgcccccag cgaggaattg
     6961 agaaagtggt tttctcatga cccggcaaag ggggatgaat ttaagcaaag gtatttccag
     7021 gggttagacc acaaaaaaga gctggtttca attgttattc aaaaagcaag ggaaggaaaa
     7081 gttacccttt tatatggagc gaaggaagaa aaatttaata acggaatggc tttaaaagag
     7141 tatattgagg ctaaaatgag ataagactta taaatattca agatgatatg gtggttggtt
     7201 atccggtgat aaaaagttaa ataggaacag gaggtaagta tgtctaaatc taaagaggaa
     7261 ctttatcagg aacgcttgaa gaggtacacc aaagcaatgc agaatgaaaa accagacatg
     7321 atccccattc gtccttttgt agctgaattt actgctcttt atgctggcta cacctgtcaa
     7381 gaagtgaccc acgactatga aaaagctttt ttagcggccc gaaaatgtgc tgctgatttt
     7441 gattgggatg cagtagtagg taatatggta tatgtatgga ctggcttaac tcaagctatt
     7501 aatctgactt attatgctac tccgggtatt gatatccctc ctgatgtagg ttttcagtat
     7561 cgagaacctc cagaagagaa agcctttatg aaacccgagg aatacgatga cctaattaaa
     7621 gaccctactg attacctttt taacgtttgg cttccccggg tttctcagga catcgttcct
     7681 ccgggaaagc caactacttt tcgtcataac ctggctttct taaaaggagg gatggctatg
     7741 atgcaatatt ttgatgcttt ggggcggcaa aatcaacttt tgcgagagga aaccggaaca
     7801 gtttctgcta tctccggcat attaaaagct ccgctggata tcttagctga taaattacgg
     7861 ggatattatg gcctggctcg agacttgatg gagcgtcccg ataaagtttt agctgcttgt
     7921 gaagccttaa tgcctcatct ttttcaagtg gctctctctg gagcagaccc agaaaaaaat
     7981 gttccggtgg ctatgtggat gcaccgtact tctgttcctt ttatcagccc tcggcatttt
     8041 caagatatct actggcctac tactaaacct attttggaag aactgtggaa taagggccat
     8101 caaactctcc tttatgccga gggaaactgg gattttcatc tttctacttt tcgggagctg
     8161 ccagcgggaa gcgttatctt ccatgtggac aggagtgaca tatttaaggc tcatcggatt
     8221 ttaggggata gattctgtat aagtggtggt attcccaaca ctcttttatc gataggaaca
     8281 cctgaggaag ttcgagacta ttgtaaaaag gttattgatg aagtggctcg agatgggggc
     8341 tacattatgg atgccagtgc catcattcag aacgatgctc aagtagagaa tgttaaggcg
     8401 ctgactgatt ttacccgaga atatggtcaa tattaggagg aataaaatgg aagaagtaaa
     8461 aagaaaaccg ggagcttgca ttccctggga ggaaaaagct aaagaactgc cacctttaag
     8521 cggagaccca gagttagtac gccgagtttg ggaagaggtt gattttctgg gttatttatt
     8581 tatctggcac tgtttggtct ctttttaggg agattgttcc agaataattc cttgatattt
     8641 attcaagatt tcatctctcc aggagaaaaa tttttcctca atctctctct ggagagaaaa
     8701 gtcttttccc ccttcctgta gaagggtaag gagcagttct ttaatctctc tggctttccg
     8761 gtcaggggga gaaagttttt ctacctcgga aggggagggt aagaatcctg tttctatctt
     8821 taaaagtatt ttctgggttt cattgtgaaa ggttataatt ttgtctaagg acgctcttcc
     8881 tggagccagg ttagggacga aaaaagttaa tatagcaaaa gctacaaagg caatggtcgc
     8941 ccagtagagc acttcttcca gagttctttt ccaccgaatc caggaactgc cagggcttat
     9001 cacccatccc aaaagcatgc cactgagtac tcctcccaga tgagcggcat tatcgatagc
     9061 aggagaaagc gcaccgatga gcaaatttat cacaatgaaa ggcaaaagac tcattcctat
     9121 ctggtgaaga gaggtattgt gatattttag accactggcg aaaattgctc ccgctaagcc
     9181 gaatatggca ccgctcgcgc ccactcccac ctgggaataa ttcagcaaat aagacagaat
     9241 gctgctactc actccggaga tgatataaat accgaaaaat ttgcgatatc catatatttc
     9301 ttctgccata cgtcctaaat aatagagtgc gtatagatta aaaaacagat gccatatgtt
     9361 tccgtggaga aaaatacaga tgagaagcct ctgccactcc ccgtgagcga cgagaggacc
     9421 aaacttagct ccccagcgga gtagattact taaagtaaag ccaccgctta aaatagtggc
     9481 taagaaaaat aaaatgttaa aagcgattat ataattaacc attaaccctg agtggttgct
     9541 ccgaaaactt cttctatttc ttttctcatt ttctcatcac tttttacata attgttgatt
     9601 tcactgctca gtttaaccag caggtataac cagtaaatag aaagagtgat aacgcttaaa
     9661 attataaaaa ctaatatgtt acgtcgggga atacgataga cctcgccagt aatggttgct
     9721 ccttttttct cttttaagta atcataaact ttatttttaa ggtcagatac tttgctcgta
     9781 gatttgaaaa tgttatgcat ataaatggcc aaaaatatga accccagcag gttgcacaaa
     9841 agagcatacc aactggtaaa aagactgatg aaatacgtta ctaagtatat ccagtaaggc
     9901 caagtgggta tgataagttc ttctattcga ttggcaaaaa atcctatttc tcctcgcagg
     9961 gggtcttcta tgtgttcttt cagataggaa aaaagctggc gggtattatt tagattctga
    10021 ttgattgccg ctgaccactg ataaagaacc actgcctgga gaatggctcc ggcaaaaccg
    10081 aataaggcaa ccacggtcca ggaaccgagg gctcctacca cccttccagt aacaagcatg
    10141 aggctaccta ttactcccag taaaacagaa agaattacaa aaatcactgc taaaattgct
    10201 actgtcatac ttacctgctt ttcttccaga aattgaaata tcaccacaat agccctcctt
    10261 ttactttatt tttttcattt taacatttat ttttctatag taccaaaaat tttttaaaaa
    10321 agtcgcttct tcagtgaaaa catttagatt ctcgccctcc tttgtcctca gcatgcccca
    10381 taattttcct ctgaacactg ttaaagtatc ccgcaaaact gattaaaat
//
LOCUS       DAOSVE010000063        10366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_3590_length_10366_cov_69.131316, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000063 DAOSVE010000000
VERSION     DAOSVE010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 10366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10366
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3590_length_10366_cov_69.131316"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1463
                     /locus_tag="PK016_06100"
     CDS             <1..1463
                     /locus_tag="PK016_06100"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HXL02673.1"
                     /translation="LKFIATKVKTQGFGDGAIVVFNPLSWERKDILHLKWGARPVEVF
                     NSKGIKVPSRIEEENGNKYLTFYVDSLPSLGYQVFYYKFSGEGEIIKSTDLKSKQTFE
                     SPFYRLEVDKDGSITGLFDKELGYQFIPDGEAMGLKVFMNKPSHWNAWNIGKDYIHRD
                     VTDLLIPESVKVIYDDSFSAQIDILYKYFNEDFDGQGRSDPEISEIRESIILYKDIKK
                     IDIKFKYSWYGMDTLVKLRVPVNYNAESTKVFGEIPYGVYERPSVPSNEYEESKYEWP
                     AQKWVAIDDSYNQIGLVLSNDGRNGFDLRECNSGVGKDTGKRVLRSSLIKHSYYHIKA
                     GDGYPTYDTPENLGIEENQEVTYSISTYRGDWRDSKAWRKGYELNYALSSVVAEPHEG
                     IYEESNSFVEIHNANCVYASVLKKAEDDDAYILRLFETGGISSPNTAIYFPMDIKRAQ
                     EANLLEEEMGEDVSVEGNCLRVNVSPWEIKTIKIFI"
     assembly_gap    1581..1680
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2173..3510
                     /gene="ffh"
                     /locus_tag="PK016_06105"
     CDS             2173..3510
                     /gene="ffh"
                     /locus_tag="PK016_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HXL02674.1"
                     /translation="MFEALTDKFSALFKRLRSKGRLSEEEVDSILKELRMVLLESDVH
                     YRVVKDLTSRVRERAVGKEVMESVTPGEMVIRILWEEIRQILGGEAEKLNLSGSFPAS
                     VMMVGLQGSGKTTTCGKLGVYLKERGYFPLLVSTDTRRPAAKEQLKILAQSAKLEFFD
                     DPQASTPQEMLKKARNYAREKSLDVILVDTAGRLHVEEELLGELKELIEEEEFSERLL
                     VLDATTGQEAVKVAQSFENWVHPTGLVLTKVDTDARGGAVLSIVAQTGWPVKLVGMGE
                     KLNQLEVFHPDRMASRIMGLGDTLTFIERVDRAISEEEKRKLEKKIEKKELDFNDFLE
                     QLQQVKNVGSFQEIMDMFPGQIRGMVSQADGEKNLKRVEAIINSMTQEERENPYIIDA
                     SRKRRIAQGSGTTVQEVNKLLKQFEDFRKLWKQMKKGRPLPFAGRKGFFGLGR"
     gene            3534..3791
                     /gene="rpsP"
                     /locus_tag="PK016_06110"
     CDS             3534..3791
                     /gene="rpsP"
                     /locus_tag="PK016_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HXL02675.1"
                     /translation="MAVKLRLSRIGRKHLPYYRIVAIDSRKARDGRPIEILGNYSPIA
                     DPPLWSLRKERIEYWISQGAEVSDKVKALLKGAEVLEAQEK"
     gene            3788..4018
                     /locus_tag="PK016_06115"
     CDS             3788..4018
                     /locus_tag="PK016_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012625423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HXL02676.1"
                     /translation="MKDLVEFIVKALVDHPEEVKVSEVEGEQSVIYEIRVAQEDMGKI
                     IGKQGRIARALRTIVKAAGVKKGKKTAVEILE"
     gene            4035..4538
                     /gene="rimM"
                     /locus_tag="PK016_06120"
     CDS             4035..4538
                     /gene="rimM"
                     /locus_tag="PK016_06120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HXL02677.1"
                     /translation="MKKVLVGKVLKARGLEGMVKILPLTDFEERFSPGSSLWVGLPDK
                     GYRLVTVAEVANEGKTVLVRFQEISSRQLAEEIQGFFLAVEEKELKVLPSDHFYYFEL
                     LGLKVMEEGELKGEVTAIIEGPSYDYLVVREGEKEHYLPFIHAYIQRVNKEKGFIEVA
                     CPGGFWD"
     gene            4535..5239
                     /gene="trmD"
                     /locus_tag="PK016_06125"
     CDS             4535..5239
                     /gene="trmD"
                     /locus_tag="PK016_06125"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008878610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HXL02678.1"
                     /translation="MKLRIDVVTLFPQAVESYLNAAILGKAQEKGIVKIKIHNLRSFT
                     LDRYRTVDDYPFGGGPGMVLKPEPLWRAVKFIESYTGSTPRVILTSPQGSLFSQEKAR
                     ELAQEEHLLFLCGRYQGVDERVITLTGAEEISIGDYILSGGELAALVMIEATVRLVPG
                     VLGDEESIKADSFYQEGLGAPQFTRPRVFQGLEVPEVLWSGNHALIKKWREKKAREKT
                     ARVRPELLPVAKYKKE"
     gene            5278..5631
                     /gene="rplS"
                     /locus_tag="PK016_06130"
     CDS             5278..5631
                     /gene="rplS"
                     /locus_tag="PK016_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HXL02679.1"
                     /translation="MDRARVLRAVESSYIKEVPEVHPGDTVRLTIKIVEGDKERFQNF
                     EGLVIAMRGSGINRSITVRKVSFGVGIERIFPLHSPSLEKIEVLRRGKVRRAKLYYLR
                     SRSSKESRIAEKREI"
     gene            5609..6172
                     /gene="lepB"
                     /locus_tag="PK016_06135"
     CDS             5609..6172
                     /gene="lepB"
                     /locus_tag="PK016_06135"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HXL02680.1"
                     /translation="MQKSEKFKKESGEPKKKSAWRELIETILWALVIALLIRYFVVEG
                     YYIPSGSMEPTLVPGERVLVAKFIYHFTEPKRGDIIVFRYPIDPHKNLIKRVIGLPGE
                     RVKIENGLIYINGVPLEGELFDRPYYDAGYYGQGERVVPEDSYFVLGDNSSNSDDSRF
                     WGYVPRKNILGRAFLVYWPPQRIKILR"
     gene            6169..6777
                     /locus_tag="PK016_06140"
     CDS             6169..6777
                     /locus_tag="PK016_06140"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HXL02681.1"
                     /translation="MMEEFLWERGYSSIGGVDEAGRGPLAGPLVAACVVLPRYCYLPG
                     VKDSKSLSPSRREKLYRDICSSSLAIGIGIVGESFIDTVGIDKANVFVFQEAISRASS
                     SRFPEILILDWVKVPFLQIPSLSLPRAESKSLAVAAASVVAKVFRDEIMQNFYQSLFP
                     HYSFSQHKGYGTSLHRREIEEWGLESCHRLSFCQKYGKQSQR"
     gene            6755..7114
                     /locus_tag="PK016_06145"
     CDS             6755..7114
                     /locus_tag="PK016_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HXL02682.1"
                     /translation="MENRAKGKRGELIAQKWVKSKLHLEILETNFRSPWGEIDIIAKD
                     RDIWVFLEVKTRESLRAGLPEESVTREKQNKIRRSALFYLQKKGYNPEEVSFRFDVLA
                     IRKEQGKLQFFYYPGCF"
     gene            7129..8661
                     /locus_tag="PK016_06150"
     CDS             7129..8661
                     /locus_tag="PK016_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HXL02683.1"
                     /translation="MLARVLSATVWGVDARLVEVEVDVASGLPAFQIVGLPDTAVQEA
                     RERVRSAVKNSGFFFPGGRVTVNLAPASLKKSGSEFDLPIALGILAATEQMKFVSPSV
                     VAMGELSLSGDIKPINGVLPVAHFLSLEDKNYRLFLARENTREGALVEKLEVYGFHHL
                     NEVYDFLQGKIELSPVTLDRESLFSAGGEGEDLSEVKGQRGGKRALEIAAAGGHHLLL
                     IGPPGSGKTMLARRLPSLLPPLSLDEAIEVTKIHSVAGLLSEEKPLIPQRPFRAPHHT
                     ISDVALIGGGQWPRPGEVSLAHHGVLFLDEALEFRRNVLEALREPLEVGKITVARAST
                     TITYPASFILTLACNPCPCGYLGDTKKTCTCSYQQIARYRAKLSGPLLDRIDLQVEVP
                     RLDFSEIASDKKEETSQEVRERVKKAREIQAERFRGEKIRLNGQMSARYLRRFSSLEG
                     EAKKLLEASMESLALSARAYHRILKVARTIADLEQSESIQVEHVAEAIHYRSLDRKYR
                     EP"
     gene            8663..9745
                     /gene="dprA"
                     /locus_tag="PK016_06155"
     CDS             8663..9745
                     /gene="dprA"
                     /locus_tag="PK016_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HXL02684.1"
                     /translation="MEDKDFWIAFNQVMGIGPIRFQKLLNVFGDAQTAWRAGVKELSE
                     VIGPHVAQKVEEERKKIEPEKLREKIESKNFKILTLKDDFYPPSLRNIPNPPFLLYMW
                     GDFDFCQFDLFISIVGTRRPTPYGRKVTRILSSELAKEKWVIVSGLALGIDGEAHSSV
                     VKEGGITVGILGSGLEVIYPSSHRKLAEEIVKKGGAIISEYPPLCPPRAENFPPRNRI
                     ISGLTQGVVVVEAPKRSGALITADFALEQGREVMAVPGSIFSPQSEGTNNLIAQGAKL
                     VQNKEDILEALGFYSGKVKKSYGEEPPLSGEEKELFSFIDSSGIFKEELLQLTGWEEG
                     KFFRILLSLELQGLVQESPGGKIFRL"
     gene            9960..>10366
                     /locus_tag="PK016_06160"
     CDS             9960..>10366
                     /locus_tag="PK016_06160"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HXL02685.1"
                     /translation="MFMVGNWLLAVAFLVILFLISWVDLRERRIPDGLVFLIVVLGII
                     SLFYGQSIFSALLGGVIYFSILFLIHLASPEGMGWGDIKLAGALGFFLGWQEALLAFL
                     LSFTGAAIIGIILIALKIKGRRDPIPFAPFMAGG"
BASE COUNT         2970 a         1750 c         2639 g         2907 t
ORIGIN      
        1 cattaaagtt cattgctact aaagtaaaaa ctcaaggatt tggtgatggt gccatcgtag
       61 tatttaaccc tctctcctgg gagaggaagg atatcttgca cctcaagtgg ggagcaagac
      121 cggtagaagt atttaattct aagggaataa aagttccctc cagaattgag gaagaaaacg
      181 gaaacaaata tttaactttc tatgtcgatt ctcttccttc tctgggttat caggtgtttt
      241 attacaaatt tagtggtgaa ggtgaaatta taaagagcac cgatctaaag agtaaacaga
      301 catttgaaag ccctttttat agattagagg ttgataaaga tgggtctatt accgggctgt
      361 tcgataagga gctcggttat cagtttatcc ccgatgggga ggcaatgggt ttgaaggtgt
      421 ttatgaacaa accgagccat tggaatgcct ggaatattgg aaaggattat attcacagag
      481 atgttactga tttacttatt cccgagagtg ttaaggtaat ttatgacgat tctttttccg
      541 ctcagattga tatactttac aagtatttca acgaagattt tgatggccag ggtaggtcag
      601 acccggagat atcagaaata agagaaagca taatattata caaagatatt aaaaaaatag
      661 acatcaaatt taaatatagc tggtatggga tggataccct ggttaaatta agagtcccgg
      721 tcaactataa tgccgagtca acaaaagtat ttggtgaaat accctatgga gtttatgaaa
      781 gaccctctgt gccttctaat gaatatgagg agagtaaata tgaatggcca gcgcaaaaat
      841 gggtggctat agatgatagc tacaatcaaa ttggtctcgt cctaagtaat gatggtagaa
      901 atggatttga cttaagggaa tgtaactccg gggtggggaa agatacaggg aaaagagttt
      961 taaggtcaag tctaattaag cactcctatt atcacataaa agctggcgat ggatatccta
     1021 cctatgatac tccggagaac ttaggaatag aagaaaatca ggaagtaact tatagcatct
     1081 ctacctatag aggagactgg agagattcca aggcttggag aaaaggctat gagctaaatt
     1141 atgctttgag ttctgtggtg gcagagcccc atgaaggaat atatgaagaa agtaactcct
     1201 ttgtagaaat acataatgct aactgtgtct atgcatcagt ccttaaaaag gcggaagatg
     1261 atgatgccta catcctgcgg ttatttgaaa ccggggggat atcttctccg aatactgcaa
     1321 tatatttccc catggatata aaaagagctc aggaggccaa tttattagaa gaggaaatgg
     1381 gggaagatgt cagcgtagaa ggaaactgcc tgcgcgtaaa cgtcagcccc tgggagataa
     1441 aaactatcaa aatatttatt taattacgtc tgggcaaaaa gaagatggcc aagacatatc
     1501 ttgaaagaag tggccatcct cttttttatt ttaaagaata caggacacaa tctttcacgt
     1561 tgataaaggg gacagtgatt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 ttattttaaa gaatacagga cacaatcttt cacgttgata aaggggacag tgattaatgg
     1741 gtggaagaag gagaaagaaa gggagtgccc tcttattcag ggtggaatcc agaaaattat
     1801 ttcactttct ctttctgtaa aaagtggccg ggatgatttc aggcaggtct cctggctcat
     1861 ggatcttcgc tcgttactct tcccttcttt tctcgtctgg tgtaaaatta taattaactc
     1921 actcctgagc atgtattttc ctcccatctt ttcaatccta ttagagggat tatagctcaa
     1981 ggggtgggtc aattttattc cggccttcct gggtcaatta tacgctggtg gtgacactga
     2041 ggaaggagaa acatgaagca tttagtaagg gaaaatataa tcgtttttag aaagaacttg
     2101 ttctagattt cttgacagag agggaagagc cgattattat ctatatgttt cagtaaaaaa
     2161 ggagagttat tcgtgtttga agctttaact gataaattta gtgctctttt taaaaggtta
     2221 cggagtaagg ggagattatc cgaagaggaa gtagactcca ttcttaagga gttgaggatg
     2281 gtgttgctgg agtctgacgt tcactatcgg gtagttaaag accttacctc tcgagtgaga
     2341 gaaagagcgg tggggaaaga ggtaatggag agcgtaaccc ctggagaaat ggtgatcaga
     2401 attctctggg aggaaattcg ccagatattg ggaggagaag cggaaaagct gaacctttcc
     2461 ggttcttttc ctgcttctgt gatgatggtg ggattgcaag gctcgggtaa aaccactacc
     2521 tgcgggaaat taggagtcta tttgaaagaa agaggatact ttcctctttt agtctccact
     2581 gacacccgcc gtcctgcagc taaagaacag ctaaaaattc ttgctcaatc ggcaaaatta
     2641 gaattttttg atgaccctca ggcttctacc cctcaagaga tgctcaagaa agctcgaaat
     2701 tacgctcgag aaaaaagtct ggatgtgatt ttagtggaca ctgcaggtag attacatgtg
     2761 gaagaagagc ttctcgggga attgaaagag ctaatcgaag aggaagaatt ttcggagagg
     2821 cttttagttt tagatgctac gaccggccag gaagcggtga aagtagctca gagttttgaa
     2881 aactgggtgc accccacagg gctcgtttta accaaagtgg atactgatgc ccggggtgga
     2941 gcagttctct ccattgtagc tcaaactgga tggccagtga aactggtggg aatgggtgaa
     3001 aaactcaacc agcttgaggt tttccatcct gatagaatgg ccagccggat aatggggttg
     3061 ggagatactc tgaccttcat tgaaagagta gaccgggcta taagtgagga agaaaaaaga
     3121 aaattagaaa agaagataga gaaaaaggag ttagatttta acgatttttt agaacaactg
     3181 cagcaggtga agaatgtagg gtcttttcaa gaaattatgg acatgttccc cgggcaaata
     3241 cgggggatgg tttctcaggc ggacggagaa aaaaacctga aaagagtaga agctataatc
     3301 aactctatga ctcaggaaga gcgagagaac ccttatatca ttgatgccag tcgtaagagg
     3361 agaatcgctc aagggagtgg cacgacagtg caagaggtga ataaattact caagcagttt
     3421 gaagattttc gtaaactatg gaaacaaatg aaaaaaggcc gtcctcttcc ttttgcgggg
     3481 aggaaaggat tttttggctt aggaagatag agatgaaagg agaagaatag aaaatggcgg
     3541 tgaaattgag attgtctcga attggtcgta agcaccttcc ttactatcga attgtggcta
     3601 ttgattctcg aaaagcgaga gatggtagac cgattgaaat cctgggtaat tattctccca
     3661 tagctgatcc acctctctgg tctttgcgaa aagagcggat tgagtactgg atttctcagg
     3721 gagcagaagt atccgacaaa gttaaagctc ttcttaaagg agcagaagtt ctggaggctc
     3781 aggaaaagtg aaagacttgg tggagtttat agttaaagcg ctggtagacc atcctgaaga
     3841 agtgaaggtc tctgaagtag aaggagagca gtcagtgatt tatgaaatca gagtagctca
     3901 ggaagatatg ggaaaaatca ttggaaagca aggaagaatt gctcgagccc ttcgcaccat
     3961 tgttaaagca gcgggagtga aaaaaggaaa gaaaacggcg gtagaaattt tagagtaggt
     4021 gatagtaaca taaggtgaag aaagtcttgg taggtaaggt tctgaaagcc cgggggttag
     4081 aggggatggt gaaaatactc cctctcactg actttgaaga gcgtttttcc ccgggaagct
     4141 ctctatgggt gggattacct gataaaggtt atagacttgt gactgtcgca gaagtagcta
     4201 acgaggggaa aaccgtcctg gttcgttttc aggaaatttc ttctcgtcag ttagcagaag
     4261 aaatacaggg cttcttctta gctgtagaag aaaaagaatt aaaagttctt ccctccgacc
     4321 atttttatta ttttgaattg ttaggtctta aagtgatgga ggaaggagag ctaaagggtg
     4381 aagtaacggc cattatagaa gggccttctt atgattatct ggtggtgaga gaaggagaga
     4441 aagagcatta cctccctttc attcacgctt atattcaaag ggtgaataaa gagaagggtt
     4501 ttatcgaggt agcatgcccg ggaggcttct gggattgaaa cttcgcattg atgtggttac
     4561 tttatttcct caggctgtag agtcttactt aaatgctgcc attttaggta aggcgcagga
     4621 aaaggggata gtgaagataa aaatccacaa tttgcgtagt ttcactttag accgctatag
     4681 aacagtggat gactatcctt ttggtggcgg accgggaatg gtgctcaagc ctgaacctct
     4741 gtggcgagca gtgaaattta ttgagagtta tactggtagt actccgcgag tgattttgac
     4801 cagccctcag gggtctttgt tttctcagga gaaagcccgg gagctggctc aagaagagca
     4861 ccttcttttc ctttgcggtc gctatcaggg agtggatgag cgagtgataa ctttaaccgg
     4921 agcagaggaa atctccatag gtgattatat tctctctgga ggagagttag ctgctctggt
     4981 gatgattgag gctacagttc gtctggtccc cggggtttta ggcgatgagg agtctataaa
     5041 agccgattct ttttatcaag aagggttagg ggctcctcaa tttactcgtc cccgggtttt
     5101 tcaggggtta gaagtaccag aagttttatg gtctggaaat cacgctctta ttaaaaagtg
     5161 gagagagaaa aaagcgaggg agaaaactgc ccgagtacgg ccggaacttc taccagttgc
     5221 taagtataaa aaggaatgat atattaagga agattttttc ctcaggagga ggttcaaatg
     5281 gacagagcaa gagttttacg agcagtagaa agtagctata tcaaagaagt ccctgaagtg
     5341 catccaggag acacagtgcg attaaccatt aagattgtag aaggtgataa agaaaggttt
     5401 caaaattttg agggtttagt gattgcgatg cgaggttcag gaataaatcg tagtattaca
     5461 gtgaggaagg tttcttttgg agtaggaata gaacgtatct ttcccctcca ttctccttct
     5521 ttagaaaaaa ttgaggttct gcgccgagga aaggtaagaa gggctaaact ctactactta
     5581 agaagtagaa gtagcaagga gtccaggatt gcagaaaagc gagaaattta aaaaggaaag
     5641 cggagaacct aaaaagaaaa gtgcctggcg agaattgata gaaaccatac tctgggctct
     5701 cgttatagcg cttttgatac gctattttgt agtagaggga tattatattc ccagtggttc
     5761 tatggaacct actttagttc ctggagagcg ggttctggtg gctaagttta tttatcactt
     5821 taccgagcct aagaggggag atatcatagt ttttcgctat cctattgacc ctcacaaaaa
     5881 tcttataaaa agagtaatag gtttgcccgg agagagagtt aaaattgaaa atggtctcat
     5941 atatataaac ggagttccct tagaaggaga actttttgac cgcccttatt atgatgcagg
     6001 ctattatggt cagggagaaa gagtagttcc tgaagattct tactttgttt tgggcgataa
     6061 tagtagtaat agcgatgata gccggttctg gggatatgtt cctcgtaaga acattttagg
     6121 gcgagcattc ctggtgtact ggccacctca gcggattaaa atattgagat gatggaagaa
     6181 tttctttggg aaagaggata ttcttctatt ggaggagtgg atgaggcagg gagaggcccc
     6241 ttagccgggc ctctcgtggc tgcttgtgta gttttacctc gctattgtta cctcccgggg
     6301 gtgaaagatt ccaagtctct ttctccttct cgcagagaaa agttataccg ggatatatgt
     6361 tcttcctctc tggctatagg gatagggata gtgggagaaa gttttatcga tacagtgggt
     6421 atcgataaag ccaatgtttt tgttttccag gaagctattt ctcgggcttc ttcctctcgt
     6481 ttccctgaaa ttttgatttt agactgggtt aaagtccctt tccttcaaat tccttctcta
     6541 tctttacccc gagcagagag taaaagtttg gctgtagcag cagcttctgt ggtagctaaa
     6601 gtgttcagag atgaaattat gcaaaacttt tatcagtctc ttttccccca ttacagtttt
     6661 tctcaacaca aaggatatgg cacttctctt caccgaagag aaattgagga atggggttta
     6721 gagagttgcc accgtttgag cttctgtcag aaatatggaa aacagagcca aaggtaaaag
     6781 gggagagctg atagcccaga aatgggtaaa aagtaagtta catctggaga ttctggaaac
     6841 taatttccgt tctccctggg gagaaataga tataattgct aaagatcggg atatctgggt
     6901 gtttctggaa gtgaaaacga gagagagctt gagggctgga cttccagagg agtcggttac
     6961 cagggaaaaa cagaataaga tccgtcgttc cgctcttttt tatttgcaaa agaagggtta
     7021 taacccggaa gaagtttctt ttcgttttga cgttttagct atccggaaag aacaaggtaa
     7081 gctgcaattt ttttattatc ccggatgttt ttagaggggg tgtaaaattt gttagccaga
     7141 gtcttgagtg ccacagtctg gggagtggac gctcgattag tggaagtaga ggtggatgtt
     7201 gcctcaggtc ttcccgcttt tcagatagtg ggtcttcctg atactgcagt gcaggaagcc
     7261 agagaaagag tgcgtagcgc agtcaaaaac agtggttttt tctttcctgg tggtagagta
     7321 acagttaatt tagcgcctgc ttctttaaaa aagagcggaa gtgaatttga ccttcctatt
     7381 gctctgggga tattggctgc taccgagcag atgaagtttg tttctccttc agtggtggcg
     7441 atgggagagt tgtctctttc cggagatata aagccgataa acggtgtcct tcctgtagct
     7501 cattttcttt ctttagagga taaaaactac cgattgtttt tagctcggga aaacaccaga
     7561 gaaggagcgc tggtagaaaa attagaagtt tatggctttc atcaccttaa tgaagtttat
     7621 gattttttgc agggtaaaat agaactgtcg cctgttactt tagaccggga aagccttttt
     7681 tctgcaggag gagagggaga agacctatca gaagtgaagg ggcaaagagg agggaaaaga
     7741 gctttagaaa ttgctgctgc tggagggcat catcttctat taatcggtcc tcctggttcg
     7801 ggtaaaacta tgttagctcg gagattacct tccctacttc ctcctttatc tttagatgag
     7861 gcaattgagg taactaaaat ccacagcgtg gctggccttc tttctgaaga gaagcctttg
     7921 attcctcagc gtccttttcg tgctcctcac catactatct ccgatgtggc tttgattgga
     7981 ggaggacagt ggcctcgccc gggagaggtg agcctagctc accatggggt tttgtttttg
     8041 gatgaggctt tagaatttcg acgcaacgtt ttagaagcct tgcgagagcc tttagaagtg
     8101 gggaaaataa cggtagctcg tgcttccact actattacct atcctgcttc ctttattctg
     8161 acactggctt gtaatccttg tccctgcgga tatttaggtg atactaaaaa aacctgtacc
     8221 tgttcttatc agcagattgc tagatatcga gctaaattgt cagggcctct tttagaccgt
     8281 attgatttgc aggtggaagt gcctcgtttg gatttttctg aaattgcttc ggataaaaaa
     8341 gaagagacct ctcaagaagt gagagaaagg gtaaaaaagg ctcgagaaat ccaggcggaa
     8401 cgttttcggg gagagaaaat ccgtctcaat ggtcagatga gcgctcgtta tctccggcga
     8461 ttctcatctc tcgagggtga ggccaaaaaa cttcttgagg cttctatgga atctttagcc
     8521 ttgagtgctc gtgcttacca ccggattctc aaagtggcaa gaactattgc cgatttagag
     8581 caatcggagt ctattcaagt agagcatgtg gcagaagcta ttcattatcg ctctttagac
     8641 cgaaaatacc gggagccttg aggtggaaga taaagatttc tggatagctt ttaaccaagt
     8701 gatggggatc ggtcctatcc gtttccagaa attattgaat gtctttggtg atgctcaaac
     8761 tgcctggaga gcaggggtaa aagagttaag cgaggtaatc ggccctcatg tggctcaaaa
     8821 agtggaggag gaaagaaaaa aaattgagcc agagaagtta agggagaaaa ttgaaagcaa
     8881 aaactttaaa attcttactt taaaggatga tttttatcct ccttctcttc gtaatattcc
     8941 taatcctcct tttttacttt atatgtgggg tgactttgat ttttgccagt ttgacctttt
     9001 tatttctata gtgggcactc gccggcctac tccttatgga agaaaagtaa ccaggattct
     9061 atcttctgaa ttggcaaagg agaagtgggt gatagtgagc gggttagctt taggtatcga
     9121 tggagaagct cacagtagtg tggtaaaaga agggggaatt acggtgggga ttttgggaag
     9181 tggtttagag gtgatttatc cttcttctca ccgaaaattg gctgaggaaa tagtgaaaaa
     9241 gggaggcgct ataattagtg aatatcctcc tctttgtcct cctcgagcgg agaattttcc
     9301 tcctcgcaat cggattatca gcggcctgac tcagggagtg gtggtagtag aagctccaaa
     9361 acgcagtggc gccctgatta cagcggattt tgctctggaa cagggaagag aagttatggc
     9421 tgttcccggt tctatttttt ctccccagag tgaaggaacc aataatctca tcgctcaggg
     9481 agcaaagctg gtacagaata aagaggatat tctggaagcc ttagggtttt attccgggaa
     9541 agtaaaaaag tcttatggag aagaaccacc gctttctgga gaagagaaag agctattttc
     9601 ttttattgat tcttcgggaa tattcaaaga ggagcttctt cagcttaccg gctgggaaga
     9661 aggaaaattc ttccgcattc tcctttcttt agagctgcaa gggttggtgc aggaatcacc
     9721 aggagggaaa attttccgtt tataagcagg tcacattata gatagcctgg agagctatgg
     9781 gtttttacat tttactgttc tgggtgtcaa tcgtgagcat ctccttccgc ccctcgcgga
     9841 agggagtgcc caggttccct gccaaatcat tgatttggca gggtgggtaa gggaagggga
     9901 aatatagagt ctcctttgaa gggtaaggga aaataaaaag aggagtaata ggagggtaaa
     9961 tgtttatggt gggaaactgg cttctggctg tcgctttttt agttattctc tttcttatct
    10021 cctgggtaga tttgcgggag aggagaattc ccgatgggct ggttttttta attgtagtcc
    10081 tgggcattat atctttattt tatggccaat ctatcttttc agcccttctg ggaggggtta
    10141 tttatttctc tatccttttc cttatccatc ttgcctcccc ggagggaatg ggatggggag
    10201 acataaagct cgccggagct ctggggtttt tcctggggtg gcaggaggca cttcttgcct
    10261 ttttgctctc ctttactgga gcagcaataa taggtataat tttaatagct ctcaagataa
    10321 aagggagacg tgaccctatc ccttttgctc cttttatggc tggagg
//
LOCUS       DAOSVE010000064        10334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_3598_length_10334_cov_78.181632, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000064 DAOSVE010000000
VERSION     DAOSVE010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 10334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10334
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3598_length_10334_cov_78.181632"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..322)
                     /locus_tag="PK016_06165"
     CDS             complement(20..322)
                     /locus_tag="PK016_06165"
                     /inference="COORDINATES: protein motif:HMM:NF024908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXL02686.1"
                     /translation="MIHYPQEERNKLYVARCLLDKKITIEEGAELLHLSERQVKRLKK
                     GVREQGDAFVIHKNRGRKPVHALSHEVKERVVNLKRSEKYSQANFSHGRGWSLRAS"
     gene            482..769
                     /gene="rpsT"
                     /locus_tag="PK016_06170"
     CDS             482..769
                     /gene="rpsT"
                     /locus_tag="PK016_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HXL02687.1"
                     /translation="MPNTRTAWKNLRKSEKRRLRNQGIKSATKTEIKKFRKLVEGGMI
                     EEAHSYLPQVYKKVDMAVSKGVFHKNKAARIKSRLTNKLNQAIAQRESSLT"
     gene            complement(762..1697)
                     /locus_tag="PK016_06175"
     CDS             complement(762..1697)
                     /locus_tag="PK016_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02688.1"
                     /translation="MEKVEFKTWIGQDIKSKYFVLELSVGKKYFEDHCWPLIVEKLGG
                     GKVVRIKGNKEWEERKGEAILPSLFGEKKILLLEDLEESLALKIKKAVSSPHFLVFLS
                     SNWKGGKEWNDSTWIKVESPRYDWENFWKESAKNKGILLDNRVLKSLSRWSKEYDWGE
                     EDVEQFLSQFPEGETIKTEDVELYFEKNERTLLFRFLDALGERNLERAIFYLRPLLQI
                     GFPPSLLLTHVSRRFRLLWQAQETKEESIDLWSGKKLNPFEWKEKILKNKNKYNLEEI
                     KRVFEVLYQVDKTLKTQNPEPETLLLGMLTRIMAK"
     gene            complement(1678..3906)
                     /locus_tag="PK016_06180"
     CDS             complement(1678..3906)
                     /locus_tag="PK016_06180"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HXL02689.1"
                     /translation="MTSFFRYPAFWVVISVLAGFLLQSYFSFPLLWLFLSLFLFTFLA
                     VLSFTRSRIFFWSIVVVWLAVGATVHALSFDLPREMILKEEGKTITIQGNLIEKNNRY
                     FITRISNFSFYSPSLLLRGGDFKPEEWGYRVRISATVRSFNSCSNPGALDFRLFWGRK
                     RIPAYLEVKNWEVVSPPQGWFSLIHWIEEKKGEFLSSWRKELGNVYPWFSALLMGVKE
                     EEFEERGDLLQEVGIYHAFCVSGLNLALLGGIILFFLNRISFIKRFSWIGALFFTFWY
                     LLFCSLSPSSFRAWLFFSLGLWGKIKGRHTLSVSLLLIAFLTMFLFQPEVVYDAGAQL
                     SFASTAGILFLMDYGKKFQNKKHFLLDYLKRGVFLTFSVYIFNLPFLIFWGFSFSSLS
                     LLGNLLVLPLLEVALFLSFLSAFLGWIVFFRPLFAFLLRNIIQASLLVSTFLQEHIPY
                     FFWDFSQVRNQLWGWIFWLSLLGLFVLLFGREKKKLVWGSVLISMLLVGGLYLFLPPP
                     LEFWVFDVGQGLATGIVEGRTMSFIDAGGIIRGYGNTGEAILKKFINYRGIEKVDYLF
                     LTHLHRDHTGGVEPLSSSFPELEVLNFFYFPDFSRFPLSSHTQIEVFPIQGDKENDQA
                     LVFRLTLPNLRILICGDIEEEGIRRLMGWGKENLKSEVVILPHHGKYVSNIPELLSLC
                     GCEAAVISCGENSYGHPNPETIKLLTDLNLPYFITQEDGAISFRPTRKNWEVKRFGKG
                     RI"
     gene            complement(3940..5232)
                     /gene="pfp"
                     /locus_tag="PK016_06185"
     CDS             complement(3940..5232)
                     /gene="pfp"
                     /locus_tag="PK016_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HXL02690.1"
                     /translation="MKDKKLAILVGGGPAPGINGVIGSVTIAARQRGLEVIGIYEGFK
                     WISNSSFNPAVHSVPLEIAQVSRIHMEGGSILRTARDSLVSKGQIDQNKVANVKKVIE
                     ELGIGYLVTIGGDDTAFSSSIVAREMKGELLVAHVPKTIDNDLPLPGNMPTFGFQTAR
                     EVATQMVKNLMEDAKTTGRWYIVVMMGRSAGHLALGVGKSAGAHLVIIPEEFSRRTVK
                     VEEVCDIIESSMIKRRALGRNDGIAIVAEGVALKFGDVEEIEKMLGRPIPRDPHGHVR
                     LAEVPLGTLLKDEMIRRFEARGEKITMVTEDIGYELRCAPPLAFDMEYTRELGYGAVN
                     YLLSPDYSLEMKKKGAMISILEGKLNPIPFPEIMDPETGRTRVRTVDINSYSYEVARS
                     YMIRLEKRDLEDEEMLTKLAQAANLSKEDFKARFSKVI"
     gene            complement(5274..5351)
                     /locus_tag="PK016_06190"
     tRNA            complement(5274..5351)
                     /locus_tag="PK016_06190"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5314..5316),aa:Pro,seq:cgg)
     gene            5910..6686
                     /locus_tag="PK016_06195"
     CDS             5910..6686
                     /locus_tag="PK016_06195"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02691.1"
                     /translation="MEKGDRENPLFEITLEREENMEGVSSMLGFDEDPFLFYQLGQKD
                     PIMSKIIEEFYGARIPRSRSLFEAAVTTVLEQQISMKVASVLRKRLVETWGRGPFSRE
                     GEEIFAFPAPWDIAKTSPEELKKIGMSSAKARTIYRLAEMIEEKEIDPESLRRLSSSQ
                     VVDFLTRLPGIGLWSAQFIVARWMSTNDCFAPDDLGLRKAIGFFYQGSEHPVSTADAR
                     EILKRFSPYERWAGWYLLMAIEKEKSSPSRNSSFQIIEER"
     gene            6694..7353
                     /locus_tag="PK016_06200"
     CDS             6694..7353
                     /locus_tag="PK016_06200"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXL02692.1"
                     /translation="MKSGLKDLFLHAKSGAENWGWRVFTDFLPVYFTEEHFENLYRRK
                     QDPWNYEHSDFEREKYLKTLQAIPKEVETIWEIGCAEGAFTQLLLEGGKKVRGVDISL
                     TALSRAKGRLKDFGDRVQLQRLDIVREDIEGTFDLILASEILYYLGGKDVLQLLEKKF
                     LRHLRPSGYLLLCHFYPSGKIIHDFYEESHRFYRVSEEITYHPQRYYIITLLKNRVWQ
                     S"
     gene            7615..9519
                     /locus_tag="PK016_06205"
     CDS             7615..9519
                     /locus_tag="PK016_06205"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXL02693.1"
                     /translation="MKMTIRSKLTIISLIPLALFILLIFAYIMPSLREVAENVMEHSL
                     LMKLQGDINSAHLFVEKYFGEIKMVNGTLVDKDGVSLEGRHDMVDDITNKLGVVATVF
                     VREGNDFRRITTSIKTADGNRAVGTMLGTDSAAYEPVMTKNLYIGEAKILGANYYTAY
                     DPIVDNGEVVGILFIGVPTDEAENIVSSGISGTSRMIITFSVAVLITSLIIIFLLGSS
                     ISHPVQVATKQAELLGEGNLSVEIPTHLLNRKDELGLLGGALKNMADKWREVVQNVIG
                     VSSHLASSSGELSSSVEEVSRATQEIARTMTQVAEGSTRQEEDLQHLSENVEGINRMA
                     QNIREKTQENVERLDTVMKERLSENSTALKRIQEEIRKATQSGAQAGEEAQKGQELLR
                     LLMEGIAAISQVTQEVAKSIAALEGRSKEIGKIVDVITEITEQTNLLALNAAIEAARA
                     GEAGRGFAVVANEVRDLAESSAQAAQQIASLIGDIQRDTQLTVKNMERAQGEVKEGAS
                     KGETVARSFSQILKTIQGVVENINQIASSSQVLEKAQKDLLSTQEETNIATSEVAKAV
                     EEISSSIQEATERVSSIVAVAEENAASSQEVSASTEEQSASLEEITSAVESLARMAQE
                     LQVTVSHFQI"
     gene            9727..>10334
                     /locus_tag="PK016_06210"
     CDS             9727..>10334
                     /locus_tag="PK016_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02694.1"
                     /translation="MYNMITMKKGKSRLNIGCVTDGIIYEYQVKAWEAIISSAQEKDV
                     NLFTFVGGRLRSGQGFDVYANQVYNLVTPRSIDGLIVLSSAIGGEVTTEEIGNFCRKY
                     LPLPVVNISLAIEGIPSILIDNRSEVKKALSHLIEDHNCRNIAFIQGTVYNQESQERY
                     QAYLETLAEYHIPVNESLIFIGGFLIGGFLRVGGREAVKVLL"
BASE COUNT         3207 a         2145 c         2159 g         2823 t
ORIGIN      
        1 aaagcctccc tcagcggaac tacgaggctc gcaatgacca ccctcgtcca tgagagaaat
       61 tagcttggga atatttttcc gaccttttca agttaactac tctctctttc acctcatgag
      121 ataaagcatg aactggcttc ctgcctctat tcttatggat gacgaacgca tcgccttgtt
      181 ctcttacccc cttcttcaga cgttttacct gacgttcgct gaggtggaga agttctgctc
      241 cctcttcaat agttatcttc ttatccagaa gacaacgtgc cacataaagt ttatttcttt
      301 cttcttgagg atagtgtatc atcctttcat tttattacta aagggacatt ttcacgagaa
      361 ttttgaagat gacattatca cagaataact acacgaggca attcgcccgc ttgcaaatat
      421 caaggctcag tggtaaaatg agcatcgtct tttttaaaaa atctttaaga ggtggaagag
      481 agtgcccaat accagaacag cctggaaaaa tttgcgaaaa agtgaaaaga gaagattacg
      541 gaatcaggga attaaatcag ctaccaaaac cgagatcaaa aagttcagga aacttgtaga
      601 aggaggaatg atagaagaag ctcattctta tctccctcag gtgtataaga aagttgatat
      661 ggcagtgagt aaaggggttt ttcataaaaa taaagctgcc cggattaagt ctcgcctcac
      721 taataagctt aatcaagcta tcgctcagcg cgagagttct cttacttagc cattatcctg
      781 gttaacattc ccaaaagtaa agtttctggc tcgggatttt gagtttttag tgttttatct
      841 acttggtaga gaacttcaaa aacccttttt atttcttcta aattatattt atttttgttt
      901 tttagaattt tttctttcca ctcaaaaggg ttgagttttt tgcccgacca taaatctata
      961 ctttcttctt tcgtctcctg agcttgccac aggagacgaa aacggcgact aacgtgggtt
     1021 aggagcaaag agggaggaaa accaatttgc aagagaggac gaaggtaaaa aatagctctc
     1081 tccaagtttc tctctcccag ggcgtccaga aagcgaaaga ggagcgttcg ctcatttttc
     1141 tcaaagtaaa gctctacatc ctctgttttt atagtttccc cttccgggaa ctgacttaag
     1201 aactgttcta catcttcctc tccccagtca tactctttac tccagcgaga aagagattta
     1261 agaactcgat tatctaataa tattccttta ttttttgcac tctctttcca gaaattctcc
     1321 caatcatagc gaggggattc cacttttatc caggtactat cgttccactc ttttcctccc
     1381 ttccaatttg aagagagaaa taccagaaag tgaggagaag aaacggcctt ttttatcttt
     1441 agagccaggc tttcttctaa atcttccagc aataaaattt ttttctcccc gaaaagagaa
     1501 ggcaaaattg cctctccctt tctttcttcc cattccttat tccctttaat ccttactacc
     1561 ttaccccctc ccagcttttc cacaattagt ggccaacagt ggtcttcaaa atatttcttt
     1621 cccactgaga gttctaaaac aaaatatttg ctttttatat cctggcctat ccaggtttta
     1681 aattctacct tttccaaatc ttttaacctc ccaatttttc ctcgtagggc gaaaagaaat
     1741 cgccccatct tcttgagtta taaaataagg taagtttaaa tcggtaagca actttattgt
     1801 ctcaggatta ggatgcccat agctattttc tccgcaagaa attactgccg cctcacaacc
     1861 acaaagggaa agaagctccg gtatattact aacgtattta ccgtgatggg gaagtatcac
     1921 tacctctgat tttaagtttt cttttcccca tcccatgagc cgtcttattc cctcttcttc
     1981 tatatctcca caaattaaaa tccgtaaatt aggtaaagtc aacctaaaaa ccaaagcctg
     2041 gtcattttct ttatctcctt gaatggggaa aacttctatc tgtgtgtgag aagaaagagg
     2101 aaaacgagaa aaatcaggaa aataaaaaaa gtttaatacc tcaagctcag gaaaagagga
     2161 agataacggc tctacacctc cggtgtggtc tcggtgaaga tgggtaagga aaaggtagtc
     2221 gactttttct ataccccgat aattgataaa ctttttaaga attgcttctc ctgtgttacc
     2281 ataacctcta attattcctc cggcatcgat aaagctcatc gtccttccct ccactatccc
     2341 ggtagccagg ccctgcccta cgtcaaaaac ccagaactct agagggggag gaaggaaaag
     2401 atagagtccc cccactaaaa gcatgctaat taaaacagaa ccccacacca gctttttctt
     2461 ttcccgcccg aaaagcagaa cgaaaagccc caggagagaa agccaaaaaa tccatcccca
     2521 tagctggttc cttacctgag aaaaatccca gaaaaagtag ggaatgtgct cttgcaaaaa
     2581 cgtagacaca agaagagagg cctgaataat atttctcaga agaaaggcaa aaagagggcg
     2641 gaagaagaca atccaaccta aaaaagcact caaaaaagag aggaatagag caacttcaag
     2701 gagaggtaaa accagtaaat tacctagaag agaaagagaa gaaaacgaaa agccccagaa
     2761 aatcaaaaaa ggaagattaa agatataaac actaaaagta agaaaaactc ctcttttgag
     2821 ataatcaaga agaaagtgct ttttattctg gaattttttc ccgtaatcca tcaaaaagag
     2881 aatcccggca gtggaggcaa aagaaagttg agctcctgca tcatagacta cctcaggctg
     2941 gaacagaaac atagtaagaa aagcaattaa taaaagagag acagaaagag tatggcgacc
     3001 ttttattttt ccccagagac ctaagctaaa aaagagccag gctcgaaaag aagaaggaga
     3061 aagactgcaa aaaagcaaat accagaaagt aaaaaataaa gcccctatcc aggaaaaccg
     3121 ctttataaaa cttatccggt tcaagaaaaa caagattatt ccacccagaa gggcaagatt
     3181 caaccccgac acacaaaaag catgatagat acccacttcc tggagtaaat cccctctttc
     3241 ctcaaactct tcttctttca cccccataag aagagcggaa aaccaggggt atacatttcc
     3301 taattctttt ctccaggaag aaagaaactc tcctttcttt tcttctatcc agtgtatcag
     3361 agagaaccac ccttgaggag gagaaaccac ttcccaattt tttacttcaa gataggcagg
     3421 gattcttttt cttccccaaa aaaggcgaaa atccagcgct ccggggttag aacaagaatt
     3481 gaaactgcgc actgtagcac tgatgcgaac tctatatccc cattcctcag gcttaaaatc
     3541 tccaccccgg agaagcaaag aaggagaata gaaagagaaa ttagaaatcc gggtaatgaa
     3601 ataacgattg tttttttcta ttagatttcc ttgaatggta atggtttttc cttcttcttt
     3661 taagatcatt tcccgaggta aatcaaaact caaagcatgt accgtggcac ctactgctaa
     3721 ccacactact actatgctcc agaaaaaaat cctgcttcgg gtgaaagata aaactgcgag
     3781 aaaagtgaaa aggaagagag ataagaatag ccagagaaga ggaaaagaaa aatagctctg
     3841 caaaagaaag cctgctaata cagaaataac tacccagaag gcaggataac gaaaaaagga
     3901 agtcatagaa aaaagggccg cataagcggc ccttgcaaat tatattactt tactaaaacg
     3961 agctttaaaa tcttctttac tcaagttagc agcctgcgct aattttgtaa gcatctcttc
     4021 gtcttctaaa tctcttttct ctaaacgaat catataagag cgggctacct cgtaagagta
     4081 agaattaatg tccacagtcc gtactctggt tcttcccgtc tccgggtcca ttatttctgg
     4141 gaaggggata gggtttagtt taccttctaa aatactgatc atggctcctt ttttcttcat
     4201 ctctaaacta taatcggggc ttaaaagata atttactgct ccatatccca gttctcgagt
     4261 gtattccata tcaaaagcca gaggtggtgc gcaccggagt tcatatccta tatcttcagt
     4321 taccatggtg attttttctc ctcgagcttc aaatcggcga atcatttcat cttttagcag
     4381 tgtacctaat ggcacctcgg ccaggcgcac atgaccatga ggatcgcgag gaataggtct
     4441 tcccagcatt ttttctatct cttctacatc gccgaatttc aaagctacgc cttcagcaac
     4501 aattgctatt ccatcatttc ttcccagggc tcttcgctta atcatggagg attcgatgat
     4561 atcacatact tcttctactt ttacggttct tctactgaac tcttcaggaa ttataacaag
     4621 atgtgctcca gcggattttc ccactcctaa agccaagtgg ccagcagaac gtcccatcat
     4681 caccactatg taccagcgtc cggtggtttt ggcatcttcc atcaggtttt ttaccatctg
     4741 ggtagccact tctcgcgcgg tctggaaacc aaaagtgggc atgttaccgg gaagaggaag
     4801 gtcgttatcg atagttttag gaacatgagc taccagtaat tctcctttca tctcccgagc
     4861 tacaatgctg gaactaaaag cagtatcatc tccaccaata gttacgagat accctattcc
     4921 taattcttct ataacttttt ttacattggc cactttattc tggtctatct gccctttgct
     4981 taccaggcta tcccgggcag tgcgaaggat agagcctccc tccatatgaa tacgagaaac
     5041 ttgagcaatt tctaaaggaa cggaatgcac tgcggggtta aaagaggagt tagaaatcca
     5101 tttgaatcct tcgtaaatcc ctattacttc taaacccctt tgtcgagcgg cgatggttac
     5161 tgagccaatg actccattga taccaggagc aggccctcct cctactaata tagctaattt
     5221 cttatctttc atttgattcc tccttccccg gaacattcgg ctaagttttt ccttggtcgg
     5281 ggcgagccga tttgaacggc cgacctcttg aacccgagtc aagcgcgcta accaggctgc
     5341 gctacgcccc gcagttcaag attataacat aaaattgctg ttccccccaa tatcagagaa
     5401 aaaaactcac tctatagtta ccccacgctc ttcaatattt atctctcctg gaaaatgaga
     5461 aatctcattg gagaaaagtg ctttagtctt aatagctttt tatactttta agtttttgtg
     5521 tcttttcatc ctgtattgtc aggccatttt gagattaaaa caataaagca atcagcttaa
     5581 ttttctcaat aaggtttaaa aaaggaaaga tactgacgca ggaagtggac acaccagaag
     5641 ttagaaacta tactataatt gtcttagaac cgcaagctga gaagacattg ttagtgcgga
     5701 ataaaaatat ctcagcctga actggagaat tttattcagt ttagtaacga gggttaacta
     5761 ttatcactcg cattgttcgt ctttgttttc cttttaccct ttttccttat ctcgaaactt
     5821 atcacccttt tttagaccgt tcttttttct ggagcagggg aagatggtgg atagcactgg
     5881 gagaggataa agtagaaata atcgaggtag tggaaaaagg agacagagaa aatcctcttt
     5941 ttgagatcac tctagagaga gaagagaata tggaaggagt ctcttccatg ctgggttttg
     6001 acgaagaccc tttccttttt taccagttgg ggcagaaaga tccgattatg agcaaaatca
     6061 tagaggaatt ttatggagca aggattcctc gctctcgaag cttatttgag gcagcagtaa
     6121 ctactgttct ggaacagcaa atctctatga aagtggcctc ggttttgcgg aagcgattgg
     6181 tagaaacctg gggtcgaggt cctttctcca gggaaggaga agaaattttt gctttccccg
     6241 ctccttggga tatagctaaa acctctcctg aggagctgaa aaagatagga atgtccagcg
     6301 ctaaggcccg gacgatttac cggctggctg agatgataga agaaaaggaa atagacccgg
     6361 aatctttgag aagattatct tcctcgcagg tagtcgattt tttaacccgt cttccgggaa
     6421 tagggctgtg gagtgctcaa tttatagttg ctcggtggat gtcgactaat gattgttttg
     6481 cccctgacga cctgggttta agaaaagcga taggcttttt ctatcaggga agcgagcatc
     6541 cagtatctac tgcagatgcc cgggaaatat tgaagcgttt ctctccctat gagcgctggg
     6601 caggatggta tctcctcatg gccatagaaa aagagaaaag ttctccttcc cgaaatagct
     6661 cctttcagat catagaagaa agatgatgaa catatgaaaa gtggtttgaa ggatttattt
     6721 ctccatgcta aatccggggc agaaaactgg ggatggcgag tatttactga ctttctgcca
     6781 gtatatttta ccgaggaaca ttttgaaaat ctttatcgcc gaaagcaaga cccctggaat
     6841 tatgagcact ctgattttga gcgagaaaaa tatctcaaaa ctctccaggc cataccaaaa
     6901 gaagtggaaa ctatctggga aataggatgc gctgagggag cttttactca actcctcctt
     6961 gaaggaggaa aaaaagtccg gggagtggat atctccctta ctgctctttc tcgggcaaaa
     7021 ggaagattaa aagactttgg tgacagggtg cagcttcaga ggctcgatat tgtaagggaa
     7081 gacatagaag gaacttttga cctcattctt gcttcagaga ttttgtatta tttaggaggg
     7141 aaagacgttc ttcagcttct ggaaaagaag tttttgcgcc atttgcggcc ttctggctat
     7201 ctacttctct gccattttta tccctcgggg aaaatcatcc atgactttta cgaagagagt
     7261 caccggttct atagagtgtc tgaggaaatt acttaccatc cccaacgata ttacatcatt
     7321 actcttctaa aaaatagagt gtggcaaagt tgactctcgg gatagcgaat tcttttccct
     7381 gaatttggca ataaaattta tccggtcaga acaaatcttt taatctcggg gggaggaact
     7441 cccgcaggca caaggtgttt aggcatgttt ttagtaatag tggtattcag cgtacacaaa
     7501 tggttgtcag gattgcctac gtcctgggat attctattgg tttaagccaa tacttcctga
     7561 aaaattatca cagacaaggg aggaaatttt tatagtaaag gaggaaataa gttcatgaaa
     7621 atgactatca ggtctaaatt gactattata agcttgatac cacttgcact gtttatattg
     7681 ttaatttttg catacataat gccttctttg agagaagtag ctgagaatgt gatggaacat
     7741 agtttgttga tgaaattgca aggtgatata aattcggcgc atttatttgt tgaaaaatac
     7801 tttggggaga taaaaatggt gaatggtact ctggtggata aagatggtgt atctcttgaa
     7861 ggcagacacg atatggttga tgatataacc aataaactgg gtgttgtagc aactgtgttt
     7921 gtaagagagg gaaatgactt tagaagaatc accaccagca tcaagactgc agacggcaat
     7981 cgggcagtag gaaccatgct gggtaccgat agcgcagcat atgaaccggt aatgacgaaa
     8041 aacttatata taggcgaagc aaaaatttta ggagcgaatt attatactgc ctacgacccc
     8101 attgtagata atggggaagt ggtggggatt ctctttatcg gtgtaccaac tgatgaggca
     8161 gaaaatatag tctcaagtgg gatatccggt acctcccgga tgataatcac cttctccgta
     8221 gcggtactga ttacaagcct catcatcatt tttctcctgg gtagctctat ttcccatcct
     8281 gttcaagtgg ctacgaaaca agctgaactt ttgggagaag gaaacctttc ggtggaaatc
     8341 ccaactcacc ttcttaatcg caaagatgag ctgggtctct tgggtggggc tttaaaaaac
     8401 atggctgata aatggcgtga agtggtacaa aatgtcattg gtgtttcttc tcatctggct
     8461 tcttcttctg gagagctctc ttcctcagta gaagaggttt ctagagccac tcaggagatt
     8521 gccagaacca tgacccaggt agctgagggc tcaactcgtc aagaagaaga tttacaacat
     8581 ctgagtgaaa atgtagaggg gatcaacagg atggctcaaa atatccgaga aaaaacccag
     8641 gaaaatgtgg agcgcctcga tacagtgatg aaagaacggc taagtgagaa ctctacagcg
     8701 ctaaagcgga ttcaagaaga aatcagaaaa gcgacccaat caggagctca agctggagaa
     8761 gaagctcaaa aagggcaaga acttcttcgt ctcctcatgg agggaatcgc tgctatatcc
     8821 caggtaactc aggaagtggc aaaaagtatt gccgccttgg agggacgttc aaaagaaata
     8881 ggtaaaatcg tggatgtaat tacagaaatc accgagcaga ctaacctctt ggctttgaat
     8941 gcagcaattg aagctgctcg agcgggtgaa gcagggcggg ggtttgccgt ggtggcaaat
     9001 gaggtgcgag atttagctga aagctcggct caagcggcgc aacagattgc cagcctcatt
     9061 ggagatattc aaagggatac ccagctgaca gtcaaaaata tggagagggc tcaaggagag
     9121 gtaaaagaag gagccagcaa aggagaaact gtagcccgga gtttttccca gattcttaag
     9181 actattcaag gggttgttga gaatatcaat cagattgcct cttcttctca agttcttgag
     9241 aaagcacaaa aggacctttt atctactcaa gaagagacca atattgctac cagtgaagta
     9301 gctaaagcag tagaggaaat tagttcttcc attcaagaag caaccgagcg agtaagctct
     9361 atagttgcgg tagcagaaga aaatgctgct tcttcacaag aggtttctgc ttctaccgaa
     9421 gagcagagtg cgtcgcttga ggaaattacc tcagctgtgg aatccttagc ccggatggca
     9481 caagagctcc aggtaacggt gagccatttc cagatatgaa agctcaactg ctgagaagat
     9541 tggtgttgtt tcattaagac aaaagataaa caactgacgc cagaagctgg aacttcagac
     9601 taaaaaaaga ggaatttata aaagttacgc attaaagctg gatatagcca aataaaaagg
     9661 accatgttga tgagccttaa agaaccactt aactcctgat ttatctggta aaagagattt
     9721 cttttattgt ataatatgat taccatgaag aaggggaaaa gccgtttaaa catagggtgt
     9781 gtgacagacg gaataattta tgaataccag gtcaaagcct gggaagccat tatttccagt
     9841 gcccaggaaa aagatgtaaa tctttttacc ttcgtaggag gaagattacg ctccgggcaa
     9901 ggatttgatg tttacgctaa ccaggtatac aacttggtaa ctccccggag tatagatggc
     9961 ctcattgtat tgagctctgc tattggagga gaagtcacca cagaggaaat cgggaacttc
    10021 tgtagaaaat acctaccatt acctgtggtg aacatcagcc tggcaataga aggcataccc
    10081 agtatattga tagataatag aagtgaggta aagaaagctc tatctcatct cattgaagac
    10141 cacaactgca gaaacatcgc ttttatccag gggactgtat acaatcagga atctcaagaa
    10201 agataccagg cttacctgga aactttagct gaataccata tacctgtaaa tgagagcctc
    10261 atatttattg gtgggttcct tattggtggg ttcctcagag tcgggggacg agaagcagta
    10321 aaagttctac tgga
//
LOCUS       DAOSVE010000065        10217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_3647_length_10217_cov_66.873450, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000065 DAOSVE010000000
VERSION     DAOSVE010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 10217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10217
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3647_length_10217_cov_66.873450"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            288..530
                     /locus_tag="PK016_06215"
     CDS             288..530
                     /locus_tag="PK016_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02695.1"
                     /translation="MCLEALGQWSTVSVQSLPSLASEIYHQSLASGMSVGKKRWQFPS
                     RWGCFLVAVLLPPFFLFKLFFAPEIGIPDQRAGGKK"
     gene            517..2802
                     /locus_tag="PK016_06220"
     CDS             517..2802
                     /locus_tag="PK016_06220"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXL02696.1"
                     /translation="MGKSEALAEEIERRETLEEELRFNLDLQRLVAGISFRFVTASSF
                     KEAVKFFLEEGRHFARADEVVLFWEGEGYIGREGKIEKIKMEGKMVSWREKLCSGGPV
                     WTEGISIPKEYWRNRESGEIKFLLSFPLEAEDRCLGFWELVWREGGSVDKIWIEAHLD
                     LFQVFSYLLNSALEREKIRKELQRERNWLETILRSIADGVIVTDLEGLINFMNREAEI
                     LTGWFLEEARGKKLEEVLVLREKDSRKPVENLVEEILRKGKLETKEPFFLRNREGKEI
                     VVTDSGAPIRRDGELQGVVLVFRDVTERQMMEEALRRSEHRYRLLFNQMLDGFALLEE
                     IYDEEGEPAFEFVFLEANPAFEHITGFSKKDIVGLTLREVLPFLDASYWELLHQVAEK
                     EEATQIEKFVLYEDKCLEIVAFSPSPGQVATIWRDVTERIKREEEIRYLGFHDALTGL
                     YNRAFFEEELQRLDKGKHFPLSLIFLDVDGLKEINDSLGHEEGDRLLREVVQVLRKST
                     REGDILTRWGGDEFVILLPQTTENDAQQIGERIEKNCEKAQGECLLPLSLSWGVATKE
                     DDTEDIREIFRRAEERTYQRKFLTRKEFFHSLISILVRRFYEEEKIRERQIRVEELIQ
                     KIGRKWGWKEEEKKKLHFLTLLWVWRRMLLPEYLKGDGYPFLREELVKIYPEFEEQLP
                     LSYSEWNSLFSEILPGEGRWKEGEETPLAARLLRVVEAYELLVEEQHLTPREAAQEIH
                     KEAGRRFDPKLAETLEEIINE"
     gene            3548..4999
                     /locus_tag="PK016_06225"
     CDS             3548..4999
                     /locus_tag="PK016_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02697.1"
                     /translation="MAFVQGRILLPCDTPWVRRAAREYMYFNKEEKETKNLLAPLPDT
                     EIKAFHTESSDLIARTRSNEEGEFFLRVPPGENYFLEFYSQSQLVALAFTTSTEEEVD
                     LGTVDSLVTARALKMKMGEKGRLVSILELTKDIEQMWARGEGLEKLVVAGSNSISDPF
                     CLIEHFDFSFLSDGKTLVVSWKSHEPVTARFYYRSFSSSCYRSMVTGRKKRGVFSLEV
                     REFEGYLFYLEVENNRHLLGRTPLRAVRAPLSPEKRKVAIEGREDGPVTVARRKVSGE
                     KMIFFPGEKESIKLFLSRALEREFEAEMYFDFLGVEEVPRFLVREGFEELELALYFPT
                     EFSFLWGPVGNNPGVFISEEDKEKISELWEDEDLALPAGKLMEIGYSTPFAEVVGYRG
                     GEYFHLFSNTDLKERVFLFSLRRLDSLDLEATIYYWGQLTLQGEGLFEGYSLELDLRG
                     RFEEGKEPLFWTFQKGEVEGEIKLWVNLEIKRK"
     gene            complement(4986..5618)
                     /gene="nth"
                     /locus_tag="PK016_06230"
     CDS             complement(4986..5618)
                     /gene="nth"
                     /locus_tag="PK016_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HXL02698.1"
                     /translation="MEERERAVHVLNALEKRFPDARLLLEFRNPFELLVATILAAQAP
                     DERVNQITKILFQRFPTPQTLSSAPLSEIEEIIQSLPYYRQKARFLKESSSLLVEKFQ
                     GIVPSEISELTSLPGVGRKTANIVLANAFNIPAIAVDTHVARVSQRLGLASSSSPSQI
                     EEDLARIIPREQWIRATHLLGFLGRTFCSAKKPRCVECPVREWCPSATSF"
     gene            6406..7110
                     /locus_tag="PK016_06235"
     CDS             6406..7110
                     /locus_tag="PK016_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HXL02699.1"
                     /translation="MRKITIWGALVLASFLLFLPCSFAQETQSTQEPKSSKEVILEKA
                     EKIRYDSAGETFVASGGVVAIQGENRIQCQELTFDLKNNKGSFKGEVIVTRGKTEIRS
                     SFMEGDFDAEIYLFKEGVKLKKEREEKEGETSFIFWEAPLLYYNGNTEEAWSEEGAEI
                     EWKETRIKADQAHYYPEKEESGEKERIVLEGKVLITEKDREMEIAKAVYYLDTEVLEA
                     EGITRARFLIEEKSEE"
     gene            7112..9100
                     /locus_tag="PK016_06240"
     CDS             7112..9100
                     /locus_tag="PK016_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02700.1"
                     /translation="MFRQGTLALFFIFLFAFSVGAQEALDLTGERLQYHPGEEEVVVY
                     EGKVTYKDVSLSASYIHIFLDKKELEAEGEVVVERGEEGAQAEKAYYNWDMDWLSLEK
                     VRSDITGKSIEGKFYFRGEEVKEEKADEDRKMEVEGAHLTSCDLEEPHYYIEAKRLVI
                     YPDEKVEIYDLSYWDFGRRLFSLPFYVFFLNRTEQLPFLPVVGHSAELGFYFSYYYNY
                     FVNDYSFGTITLDWWQHGGWGLGVTHYLEREGEDGRLTIYYRDPASSSSALRGSVKYS
                     KEVDEFTDLSLSVDYSGTLGEADERYSGFLSVLRAKDKSTSKLNLSYSANESSATESL
                     YTTWNYQYKLREDLTGELSLNYREYEKRREFTDQDLAYGITFKKTSDLYAYYLRYGGH
                     IDLEGDAYTGDSNKVVFKIPELEVARSKERIGESDFTYKAGLLLGHYFEEETGVIDER
                     LRLTFNLEGSSKVGGGNLTSFFNFQQDFYGNGFARYIWLGEIKWADEISSDLFLTLSY
                     QKGSYDGATPFKFDYVRPRTDYLNLGLDYARSPWEVSLDTGYDFREEEFVDALLSVEY
                     NLDDKKKVELSGSYDISNEEWGSVVFGISWPMNKEWGIDFSGRWDPQGGKIDGVRVGL
                     TDDLHCREISLFYDQSRDTFWLEYGIKALPGEKIKLGG"
     gene            9105..9611
                     /locus_tag="PK016_06245"
     CDS             9105..9611
                     /locus_tag="PK016_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02701.1"
                     /translation="MKSKFFLLVVVALLVVGCARGEQPPWEEGKNLYITTTWQEISSS
                     YIYYIVISADSVAPSTDPRSWNSFYVVKWEDHNFFLRKPGESTFSYFSSPSVSDQEMR
                     VTLSLIDVLDDPAILRIMVVSTDRGGNVEDSLDKGAVIFRPRDESSKSWNDDDDTGGL
                     LSLKIEAR"
     gene            9612..>10217
                     /locus_tag="PK016_06250"
     CDS             9612..>10217
                     /locus_tag="PK016_06250"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HXL02702.1"
                     /translation="MKKRNGKLFWIVFIPFLFIGCFNSGVLPPVVDKGTSWTVMVFLN
                     GDNDLYNYAWRDFDSLEIVGSTPQVKVVIQFDPFWGEAARYLVEKDTIPNYIGSPVLE
                     YLGEVDMGDGSELANFVHFCAENYPANKYALIIWDHGTGFKSKDISFDISPEDSITIP
                     ELEKALSLSTMYLGKKIDFLGMDACLMAMVEIAYEVKNHARV"
BASE COUNT         3038 a         1802 c         2612 g         2765 t
ORIGIN      
        1 aaaatctaat atactaagga gggaggaatt cctccctcct tagtatattt cctctcatct
       61 ttttgcctcc tcttgagaaa acattctttc agttataaaa ttattgcaag atagagaggg
      121 gttttctgaa aatgagaggg gagaaaaaag caaaaaaagt tagtaaacat ctttttagtt
      181 tttcttctct ttttgcctct ccgaggagag gcaaaaagag gtttcttcgt ggaagatggt
      241 gagagccccc ttcggttttc caggagggag aagggagggc acgggaaatg tgcctggaag
      301 cgttagggca atggtccact gtttctgttc agtctttacc tagtctggct tctgaaattt
      361 atcaccaatc tttagcttcc gggatgagcg taggaaagaa gagatggcag tttccctccc
      421 ggtggggatg ttttttagtt gctgttttgc ttcccccttt ttttctgttt aagttattct
      481 ttgcgccgga aatcgggata ccagaccaga gagctggtgg gaaaaagtga agcgcttgct
      541 gaggaaatag aaaggcggga gaccttggaa gaagagctgc gctttaacct tgaccttcag
      601 agacttgttg ccggtatttc tttccgtttt gttactgctt cttcttttaa agaggcagta
      661 aaatttttcc tggaagaagg caggcatttt gcccgggcgg atgaggttgt tcttttctgg
      721 gagggagagg gctacattgg gagggaaggg aaaatagaaa aaataaagat ggaagggaag
      781 atggtttcct ggagagaaaa gttatgttca ggagggccag tgtggacaga agggataagt
      841 attcccaagg aatactggag gaacagggaa tctggagaaa ttaagtttct cctttctttc
      901 cctctagaag ccgaagatcg gtgtctgggg ttctgggagt tagtctggag agaaggaggg
      961 agcgtagata aaatatggat agaagcgcac cttgaccttt tccaggtttt ctcctatctt
     1021 ttgaacagcg ccctggaaag agagaaaata agaaaggaac tgcagcgaga aagaaactgg
     1081 ctggagacca ttcttcgctc cattgctgat ggcgtgatag ttaccgattt ggaggggctg
     1141 attaatttta tgaaccggga ggcagaaatt cttactggtt ggtttttaga agaagcccgg
     1201 gggaagaagt tagaagaggt tcttgtcctc cgggaaaaag atagtaggaa gccggtagaa
     1261 aacttggtag aggagattct gagaaaagga aaactggaaa cgaaagaacc cttttttttg
     1321 aggaatcgag aagggaaaga aatagtagtt accgatagcg gagctcctat tagaagagac
     1381 ggggagctcc agggagtagt gctcgtattt cgggacgtta ctgaacgcca gatgatggaa
     1441 gaggccctcc gcaggagcga acatcgctac cgcctgcttt ttaaccagat gttagatggg
     1501 tttgcccttc tggaggagat ttatgatgaa gagggagagc ccgcttttga attcgtgttt
     1561 ttagaggcta atcctgcttt tgagcatatt actggcttta gtaaaaaaga catagtagga
     1621 cttactcttc gagaagttct tccttttctg gatgcttctt actgggagct tcttcaccag
     1681 gtggcagaga aagaagaagc cacccagata gagaaatttg ttctttatga agataaatgc
     1741 ttagaaatag tggctttttc tccctctcca gggcaggtag ctactatctg gcgagatgtt
     1801 accgagagga taaaaagaga agaagaaatc cgctatcttg gctttcacga cgctttgact
     1861 ggtctttata atcgggcttt ttttgaggag gaattgcagc gcctggataa aggaaagcac
     1921 ttccctctga gcctcatttt tcttgatgtc gatggcttga aggaaataaa cgattctctg
     1981 gggcacgaag aaggagatag attgttgaga gaagtagtgc aagtacttcg aaaatctacc
     2041 cgggaagggg atatcctcac ccggtgggga ggagatgaat tcgtcatcct tcttcctcaa
     2101 accactgaaa atgatgctca acagattggg gagagaatag aaaaaaattg tgaaaaggct
     2161 caaggggaat gccttcttcc tttgagcctt tcctgggggg tagctaccaa agaagacgat
     2221 acggaagata taagagaaat ttttcgcaga gcagaagaga gaacttatca gcgaaaattc
     2281 ttaactcgca aggaattttt tcattctctt atctctattc tggtacgccg attttatgaa
     2341 gaggaaaaaa ttcgggagcg ccagattagg gtggaggaat tgatccagaa aataggaagg
     2401 aagtggggat ggaaagagga agagaagaaa aagctacatt ttttgactct tctctgggtc
     2461 tggagaagga tgcttcttcc tgaatacctg aaaggagatg gctatccctt tttacgggag
     2521 gaactggtta aaatttaccc tgagtttgag gaacaactcc ctctttcata ctcagagtgg
     2581 aactccctgt tttcagaaat tttacccgga gagggacgct ggaaagaggg agaggaaact
     2641 cctcttgcgg ctcgtctttt aagagttgtg gaggcttatg agctcctggt tgaggaacaa
     2701 cacctgaccc cccgggaagc ggcgcaggaa atccacaaag aagcaggaag gcgcttcgac
     2761 ccgaaactgg cagaaacatt agaagaaata ataaatgaat aaaggacgat ggctcgctga
     2821 cccggtgctt gaggaattgt ttcaatcact gtatgtattg acttttatag ttaaccgcaa
     2881 aagacaattt gtggcgcccc cattttgtca tcgcgaggag cgggacagcc cacggcgtaa
     2941 gataaaaaag caaattagat aagaaaaaat cttgataaaa agagatagag caataagggg
     3001 gtttaccctc ttattgctcc ccaaaagcaa aagaaaagaa caaagactta aaaagcaaat
     3061 aaacaaaaac tactggtggg aaaatatcca ccagtatgct gggaaaaaac ccagcgttga
     3121 gaaagaaaaa gccttcttca gtgcttgcga ttgaccgttt ggagaggatt gccacacccg
     3181 gcaaaaaccg ctgccttgtg tccagaccag atagatattg gtaatgatga gctcataacc
     3241 ccaaactata aaggagagtg cccctttata atccccccaa aaacaaaaac ataaaagtaa
     3301 aagactacta aagactatag tatccttctg tgtttggcga ttccattttt gacttccgat
     3361 gtaatttact ttttaaaagt tttaataatt taacaggttt tgcgggatac tataatcttt
     3421 aagcaattcc cgaatatcag ttgtatcctt tcttttcggc ttagaataag aggtgatata
     3481 atttagtttg tgatattatt tggtattatt ctcaaaaggt gtagctttta aagaagaggt
     3541 gattactttg gcttttgttc agggaaggat tcttctccct tgtgatactc cctgggtaag
     3601 aagagcagcc cgggaatata tgtattttaa taaagaagaa aaagaaacaa aaaatctttt
     3661 agctcctctt ccagatacgg agatcaaagc cttccatacc gaaagttctg atttaatagc
     3721 ccggacgagg agcaacgagg aaggagagtt ttttctgcga gttcctcctg gagaaaatta
     3781 ttttctggag ttttattctc aatcccaatt agtggctctg gctttcacta cttctactga
     3841 agaggaggta gatttaggca cagtcgatag cctcgttact gcccgggctt tgaaaatgaa
     3901 gatgggggag aaagggagac tggtctcaat ccttgaactt actaaagata tagaacaaat
     3961 gtgggcgagg ggcgaggggt tagaaaagct cgtagtggct ggtagcaatt ctatctctga
     4021 ccctttttgc ttgatagagc attttgactt ttcttttctc tctgatggga aaactctggt
     4081 agtaagctgg aaatctcacg agccagtgac ggcccgcttt tattatcgct cttttagttc
     4141 ttcttgctat cgtagcatgg ttactggacg aaagaagaga ggagtatttt cccttgaggt
     4201 tcgagaattt gaagggtatc ttttttactt agaagtggaa aataaccgcc atcttctggg
     4261 gagaactcct cttcgggcgg tgcgcgctcc cctttctccg gaaaaaagaa aagtggccat
     4321 agaggggaga gaagacggac ccgtcactgt ggctcgtcgt aaagtttcag gagaaaagat
     4381 gatttttttt cctggggaaa aggaaagcat taagcttttc ctctcccggg cgctggagag
     4441 agagtttgaa gcagaaatgt actttgattt tctgggagta gaagaggtgc ctcgttttct
     4501 agtgcgagag ggcttcgaag agctggagct tgccctttat tttcccactg aattttcttt
     4561 cctgtggggg ccggtgggga ataatccggg agttttcatc tctgaagagg acaaggagaa
     4621 aatttcagaa ttatgggaag atgaagacct tgctcttccg gcggggaaat tgatggagat
     4681 aggctatagc actccttttg cagaagtggt tggttatcga gggggagaat atttccatct
     4741 tttttctaat accgacttaa aggaaagggt gtttcttttt tctctgcgcc ggttggactc
     4801 tctggatctg gaggccacca tttattactg gggacaatta actcttcaag gagaaggact
     4861 atttgaaggt tattccttag agcttgacct ccggggcagg tttgaagaag gtaaagagcc
     4921 tcttttctgg acctttcaga aaggcgaagt ggaaggagaa attaaattgt gggtgaatct
     4981 ggagattaaa aggaagtagc agaaggacac cattctctca ccggacattc tacacagcga
     5041 ggttttttgg cagagcagaa tgtccggcct aaaaagccta agagatgggt tgctcttatc
     5101 cattgctccc gaggaattat tctagctaaa tcttcttcta tttgagaagg ggaggaagat
     5161 gaagcaaggc ctaatctctg agatacacga gccacgtgag tatccacggc aatagctgga
     5221 atattaaaag cattggccaa aacaatgtta gcagtttttc tccctacccc gggtagggaa
     5281 gtgagttctg aaatctcaga gggaacaatc ccctggaatt tttctactag aagggagctc
     5341 gattctttga ggaaacgggc tttttgccga tagtaaggaa gagattggat gatttcttca
     5401 atttctgaaa gaggagcact tgagagagtc tggggggtgg gaaaacgttg aaaaagaatt
     5461 ttagttatct ggtttacccg ttcgtcagga gcttgagcag ctaaaatggt ggccactaaa
     5521 agctcaaaag gattgcgaaa ttctaaaagc aaacgagcat cagggaatcg cttttctaaa
     5581 gcattcagaa catgaaccgc tctttccctt tcttccattt ttacccttct ttccgatttt
     5641 aaatgtcttg agtaaattat aagcgaaatt gtaataagag aaaagataag agagggtgag
     5701 gttatctttc ctcaaaaaac ctcttctttt ccgtcgctgc aatacctcgt gtctgtcatt
     5761 cctgaatgtc cccgtcagga atgacagact ataggggagg aagcccctta tagctccccg
     5821 aaaaagtcag aagaaaagta aaaacaatca aggactgttg aggaagaaaa aaacagacaa
     5881 cagcctgcga tgcaggataa gaacataaat aaaaatacaa aagatcactg gtggaaaaat
     5941 catccaccag taaaaatctg tggatgcctg ataaaagatt tcaggcatga cagaaaagaa
     6001 ctataaaggg gagtgcccct ttataatccc ccaaaagata aaggaaaagg ataagaaact
     6061 taaaaaaaca aagactactg agggagaaaa aacgtctccc tcagtgagga gtgccccttt
     6121 ataatccccc ggaaaagatt gaaagacaaa taaaaaacat aaagaaacaa aatccgctga
     6181 gagagaaaaa acttcttcct cagcggattc ccgattgaag cgctcgggaa tggcgaagaa
     6241 agggaattta tccagagaat aaaaacgaga ttgccacacc atgcgagaaa gtaagatctg
     6301 cgacaacctc ttaagagaag aagcaccccc agcaccggag ggaattacag ctatgcatac
     6361 ttaatggtat aataaccaaa aattggggtt aagcgaggga taaaattgag gaaaataaca
     6421 atatggggag ctctggtttt ggcctccttc cttcttttcc ttccctgttc ttttgcgcaa
     6481 gaaactcaga gtactcagga acctaaaagt agcaaagaag taattttaga aaaagcggaa
     6541 aaaatcagat acgactctgc cggagaaact tttgtcgctt ccgggggagt ggtggctatt
     6601 cagggggaga atcgcattca atgtcaggaa ctcacattcg atcttaagaa taataaaggt
     6661 agctttaagg gagaggtaat agtcacgaga ggtaaaacag agattcgctc ttcttttatg
     6721 gagggtgatt ttgatgctga gatttatctc tttaaagaag gggtaaagct gaaaaaagaa
     6781 agagaagaaa aggagggaga aacttctttt atattctggg aagctcctct tctttactat
     6841 aacggaaaca cggaagaagc gtggagtgaa gagggggcag aaattgaatg gaaagaaacc
     6901 agaataaaag ccgaccaggc tcactattat ccggaaaaag aggaaagcgg ggaaaaagaa
     6961 agaatagttt tggagggtaa ggtgctcatc accgaaaaag accgagaaat ggaaatagct
     7021 aaagcagttt attatctgga tacggaagtt ttggaagcgg aagggattac ccgtgcccga
     7081 tttttaattg aagaaaaaag cgaggaatga agtgtttcgc caggggacgc ttgctctctt
     7141 ctttatcttt ttgtttgctt tttctgtagg ggcgcaagaa gctctcgatc taaccggaga
     7201 acgccttcag tatcatccgg gagaagagga ggtagtggtc tacgagggga aggtgaccta
     7261 taaagatgtt tctctttctg ccagctatat tcatattttt ttagataaga aagagttgga
     7321 agcagaaggg gaagtagtgg tggaaagggg agaagaggga gcccaggcag aaaaggctta
     7381 ttataactgg gatatggatt ggctgagttt agaaaaggtg cgctctgaca ttactggcaa
     7441 atctattgag gggaagtttt attttcgagg agaagaagta aaggaagaaa aagcggacga
     7501 agatagaaaa atggaggtgg agggagctca tttaacgagc tgcgatttag aggagcctca
     7561 ttactacata gaagctaaaa ggttggtaat ttatcccgat gaaaaagtag aaatctacga
     7621 cctttcttat tgggactttg gccgtcgcct cttttctttg cccttttacg tttttttctt
     7681 aaatcgtacc gaacagttgc ccttcttgcc tgtggtggga cattcggcag agctgggatt
     7741 ttattttagc tattattaca attattttgt aaatgactac tcttttggca ctatcaccct
     7801 ggattggtgg caacacgggg gatggggtct cggggtcacc cactatttag agcgagaagg
     7861 agaagatggg aggctcacca tctattatcg agacccggct tcttcttcct ctgctctccg
     7921 ggggagcgtt aaatacagca aagaagtcga tgagtttact gatttgagct tgagcgtgga
     7981 ctattccggc accctgggag aagctgatga gcgctattcc ggttttctct ctgttttgcg
     8041 agcgaaagat aaaagcacca gtaagcttaa cctgagctac agcgctaacg agagcagtgc
     8101 aactgaaagt ctctatacta cctggaacta tcagtataag ctgagagagg acctgactgg
     8161 agaattgagc ctcaactaca gggaatatga aaaaagaagg gagtttaccg accaggatct
     8221 cgcctacggg attactttta agaaaacttc tgacctttat gcttattatc tacgatatgg
     8281 tggccacata gacttagaag gagatgccta tactggcgat tccaataaag tggtatttaa
     8341 aattcccgaa cttgaggttg ctcgaagcaa agagaggatt ggagagtcag attttaccta
     8401 taaagcggga ttgctcctcg gacactattt tgaagaagag actggagtaa tagatgaacg
     8461 tctgcgtctc acttttaacc ttgaaggtag tagcaaggtg ggaggaggaa atctcacttc
     8521 ttttttcaac tttcaacagg atttttatgg caatggcttt gcccgctaca tctggttggg
     8581 agagataaaa tgggcggacg agatatcttc tgatttattt ttaactcttt cttaccagaa
     8641 gggtagctat gatggagcta cccctttcaa atttgactat gttcgtcccc gaactgatta
     8701 tttgaactta ggcctagatt atgcccggag cccctgggaa gtgagcttag ataccggata
     8761 tgattttcgg gaggaagagt tcgtagatgc tttgttgagt gtagagtata acttagacga
     8821 taagaaaaaa gtagaattga gtggtagcta tgatatctct aatgaagagt ggggaagcgt
     8881 ggtatttggt atttcctggc ctatgaataa agagtggggt attgattttt cagggagatg
     8941 ggaccctcaa ggaggaaaga tagatggagt gcgagtgggg ttaactgatg acctccactg
     9001 tcgggaaata agcctttttt atgaccagtc ccgggatact ttctggttag agtatggaat
     9061 aaaagccctc cccggagaga aaataaaatt aggaggttga atcgatgaaa agtaaatttt
     9121 ttcttcttgt cgtggtggcg cttcttgtgg tgggttgcgc tcgaggagaa caacccccct
     9181 gggaagaggg gaaaaatctc tatatcacta ccacctggca ggaaattagc tcttcttata
     9241 tttactatat agttataagc gctgactctg tagctccctc cactgacccc cggagctgga
     9301 atagttttta tgtggtgaag tgggaagacc acaacttctt tttaagaaaa cccggtgaga
     9361 gcacctttag ctacttttct tctccttctg tgtcagacca agaaatgagg gtaacgttat
     9421 ctttaattga tgttttagat gaccctgcta ttctccggat aatggtggtg tcaacggata
     9481 gaggaggaaa tgtagaggat tctctggata agggagcagt gatatttcga cctcgggatg
     9541 agagtagtaa atcctggaat gatgacgacg atacaggagg ccttctctcg ttaaaaatag
     9601 aggcaagata aatgaaaaag agaaacggga aacttttctg gatagttttt atcccttttt
     9661 tatttatcgg ctgctttaat agcggggttc ttcctccggt ggtggataaa ggcaccagct
     9721 ggacggtgat ggtttttctg aatggagata atgacctgta taactatgct tggcgagatt
     9781 ttgactcttt ggaaatagtg ggctcaactc ctcaggtgaa ggtggtaata caatttgacc
     9841 ctttctgggg ggaagcagct cgttatcttg tggagaaaga taccatacct aactatattg
     9901 gttctccggt tttggaatat ttaggggagg tagatatggg agatgggtca gagcttgcca
     9961 atttcgtgca tttttgtgcg gaaaactatc cggccaataa atatgctctc atcatctggg
    10021 atcatggcac aggatttaaa agtaaagata tctcttttga tatatccccc gaggattcta
    10081 ttactatacc ggagctagag aaggctttgt ctttgtctac tatgtactta gggaagaaaa
    10141 tcgactttct ggggatggat gcctgtctta tggcgatggt ggaaattgct tatgaggtta
    10201 aaaatcatgc ccgggtg
//
LOCUS       DAOSVE010000066         9884 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_3755_length_9884_cov_64.149150, whole genome shotgun sequence.
ACCESSION   DAOSVE010000066 DAOSVE010000000
VERSION     DAOSVE010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 9884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9884
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3755_length_9884_cov_64.149150"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..495)
                     /locus_tag="PK016_06255"
                     /pseudo
     CDS             complement(79..495)
                     /locus_tag="PK016_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(612..1583)
                     /gene="pdxA"
                     /locus_tag="PK016_06260"
     CDS             complement(612..1583)
                     /gene="pdxA"
                     /locus_tag="PK016_06260"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HXL02703.1"
                     /translation="MNKLSLILTMGDPSGVGPEIVVKSIIKWDVEAVPIVVGDRKILQ
                     KAEEMQGVKIPWSDFPSSRGVGPFLCDLDNVDLSSFSWGKISPSMGRASYEYIEKAIE
                     LMKEGRGEGVVTAPINKEAIHEAGIPFIGHTEIFQELTHSEEALTMFQVKNLRVFFFT
                     RHLPLKEAIEKIKKDKIAEFIPRMHYYLQRLGLAHPRIAVAALNPHGGEGGLLGEEEK
                     REIIPALSVVRDTGIEILGPYPADSIFWRTFQGEFDAVLSLYHDQGHIATKCLDFYKT
                     VSVTLGLPFIRTSPDHGTAMDIAGKGIANEESMLEACRHAAQYAVYY"
     gene            complement(1580..2731)
                     /locus_tag="PK016_06265"
     CDS             complement(1580..2731)
                     /locus_tag="PK016_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HXL02704.1"
                     /translation="MIDIPERIVNLPRSGIRKLMEIALRDPEVIRLEMGEPHFSTPHY
                     ILEKLKDFYWQGEIKYTPTVGIPSLREKIAHHLKEEKGWKVTPEEVVILPGSLFGTVT
                     IFSSLLEPQDEVLVPDPGFTNHFYQVELCGGKMERYHLQEKNGFLPDIKEIENLITSR
                     TKMILINSPSNPLGVVFPKETLCQLAQLAEEYGLLLISDEAYEKYVYEGEHQGMFSFI
                     NEDNLISIFSFSKTYALTGWRVGYMIVPPYLVKHLAKVEEYLIACTSHLSQKAAEIAL
                     DLPKEEVKKMVDYYQGNRDKAIELLEQGGFHFFKPQGGYYLWIDIQEFGLSSLDFCKT
                     LVKESKVALSPGDAFGEGGEGFVRLSICRKREEVEEGVKRLVEFRKGRK"
     gene            complement(2744..3397)
                     /gene="fsa"
                     /locus_tag="PK016_06270"
     CDS             complement(2744..3397)
                     /gene="fsa"
                     /locus_tag="PK016_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HXL02705.1"
                     /translation="MKFFIDTADVGEIKEALKLGILDGVTTNPTLVSKTGRGFREVIM
                     EICSLVPGPVSAEVVSLKAEGMIKEGKELSTWAPNIVVKVPITAEGLKAIKALSQEGV
                     KVNTTLIFNPLQALMAAKAGATYVSPFVGRLDDISSDGMHLVADILQIFCNYDITTQV
                     IVASIRHPMHVIEAAKLGAHVATIPFSVISKLIKHPLTDIGIEQFLADWAKVPEKPF"
     gene            3499..4551
                     /gene="purC"
                     /locus_tag="PK016_06275"
     CDS             3499..4551
                     /gene="purC"
                     /locus_tag="PK016_06275"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012940063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HXL02706.1"
                     /translation="MQKRGVYNMGSVKDLIVLSSPHEEEPGRGKFIFSDRYSVFDWGE
                     MPDHIPLKGASLCLIGAYFFERLEEAGFLTHYQGVEEEGKLKRLSDLKSPTNQMIVSL
                     VRVIQPPEMEGKYDYNIYQGVRGNFLIPFEFIYRNSLPAGSSLRKRLEKGEIKLEDYG
                     WDKIPAPEERLSPPIVDVSTKLEEQDRYLSWREVEETGILQKEEIEKIRQVILAIDDL
                     ITKEVEKINLKNEDGKIELALDKDRNLVVVDVVGTPDESRFTYRGMHLSKEIAREFYR
                     KTPWYEEVQKAKLQNPVGWRGLVKTSPPPLPSSLLQLISWVYERIAMDLTNRDWFPNV
                     PSLEEIGEIIGQELLI"
     gene            4597..4672
                     /locus_tag="PK016_06280"
     tRNA            4597..4672
                     /locus_tag="PK016_06280"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4630..4632,aa:Arg,seq:cct)
     gene            complement(4781..6259)
                     /locus_tag="PK016_06285"
     CDS             complement(4781..6259)
                     /locus_tag="PK016_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02707.1"
                     /translation="MIFKETSAFPLLEEAEKLENLHLRSSRAYLLTVIIMFFLFIGIE
                     IVQGEELLSYPSLIRELHSLWKEGREDITYLLIEDTPLFLDHRELTGEIRKIVENREY
                     PFYLRKSALSFLSRQMEGKERGEYLISLFQREPSVAIRVEIIRLLGETDDWIPFLFSI
                     LQGGNSSNEKEEVVLQWEALRALAHWKVRESLPFFFRLVEKEDFPPELKEEADELLSL
                     YDFSVSSYLLAMTNSENPFFREIALFTLWERGDNNIMSYAYKMSNDQDKKVSNLSQAI
                     LWGEGKIPHLVLQGEDLDSRLIRVLTQYERGVNLLIEIGEKDPMLEEQIFESLGEIED
                     IAFTSLLKEKLKEREWRPLSLLRFLPQGERAEIIFSLLASSGEELYLQEEEIPPLSKE
                     DLRSNLYLIRETLLSGSERAQLFILRRLPILGEEALPLLEIATRETSLALQREIIRAL
                     EEIEAPATIYFLERLAFSPYEEIEIRARLALAKKYLSQGYSW"
     gene            complement(6249..7274)
                     /locus_tag="PK016_06290"
     CDS             complement(6249..7274)
                     /locus_tag="PK016_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HXL02708.1"
                     /translation="MATIYDIAKLAGVSPATVSRALNQKSQVKDETRKKILEIAEELN
                     YSPNYLARSLKKKQTFTIALIISDVTNPFFTTLARGVEDKASSQGFNTIFCNTDENPA
                     KEKEYVDLMLRRRVDGLVIASCSNGDTLRKLKGKEVSVVLVDRKIEGFDGDLVMGDSK
                     EGAYELTRHLIEVHNLREIAMITGPITISTSKDRVAGYQRALQEAGIPVKPEWIMVGS
                     YKEDFGYQIATRFFSEKKKLPRAVFAGNNFIAIGIVRAARERGIKIPEDLALVTFDDL
                     ETTSLLYPFLTVAKQPAYTMGSLATQFLLERIEKKEIREKREVVLKVEIIIRRSCGCK
                     GSENNDF"
     gene            complement(7377..7961)
                     /locus_tag="PK016_06295"
     CDS             complement(7377..7961)
                     /locus_tag="PK016_06295"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXL02709.1"
                     /translation="MLLDLALSPGKVKEFAVRRTDFMLGIAERQIELAKPVGMVVWGD
                     VAYDKGMFFSPRLWQDIYFPCVKRIYDFLHEKGLIIVYHGCGRSLEIMENLIEAEINV
                     YNPLEAKAGMDPAELKKKFGDRLSFWGGIDTRLLGYGTWEEIEKEALYKLQAARGEGY
                     MPASDHSVASNIDPLIYDKLINLLKEKGRYPLPI"
     gene            complement(7946..8431)
                     /locus_tag="PK016_06300"
     CDS             complement(7946..8431)
                     /locus_tag="PK016_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02710.1"
                     /translation="MPLGDFYWTEFLNRWREKFNLPSDTDIYEYYDLDIKVISPNMDP
                     KVESCQIIEETPEYVVFKSGFGCTVKKLFAAPMPMFLDFEVKSADEFANFTFDDPRDD
                     RRYFEKCCGIINCGDSFGTIESYAEAIENNKNKFCIFGSICEPHETMGAFGEPKGCYL
                     I"
     gene            complement(8515..9198)
                     /locus_tag="PK016_06305"
     CDS             complement(8515..9198)
                     /locus_tag="PK016_06305"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HXL02711.1"
                     /translation="MRKGVKHLSNILGKETFPFALEAKGKEFLLHEPRVKQMLGLGYA
                     VSPSGPDTFVVEHDTDFDENAPQLFLDNIQALGFLERLPAPSLEAKKVRMFYYLDQFF
                     SFMDALGLCIFAFAPCRFLPFSLISPLIQAITGWEMSLFRLMKIGETRMQLERLFNLQ
                     EGIEKKEDWLPERFFEPVKSGPRRSLRINPQDLKEAIELFYSFAGWNIDNGFPERGKL
                     EELAISEYL"
BASE COUNT         3006 a         2072 c         1722 g         3084 t
ORIGIN      
        1 tagtggagta aacaaaaaag gagtacctct atcccttgat atatcaatgt ttttagatta
       61 cagctgtcaa agtcaggtat atacttcgcc catttgtgaa acggcatgga gtgttgtctg
      121 atctgcctga aaatgtgttt ttctatatcc tcctctaagc aataaatttt cagctacata
      181 agcaaattca ggtctaacca taggttcgct tagttgtcta ataggtttaa gaaaaagacg
      241 gtaaggaaaa tagtatgttc taccagacat tttgaaggta attgaggacc aaggtggcaa
      301 gttttcagca tttttgaaag ctttcttttc aataatctga tatagattaa acactctcgc
      361 accgtataaa aatgaagcaa aatccccttc tgcaatttcc ataaatgtcc atagcccatt
      421 tatactattc gtaaaacctg caagcgcata tttaacacat aagtccaaat taaccctatt
      481 tgagactgaa atcataaaat atgccactta actcaccccc cctaaaaagc ccataatctt
      541 gtagtttata ttgtcaattg ataatggggt tcttccaatt taaggctaac atctatgccc
      601 ttgactccga tttaataata tacggcatac tgagcagcat ggcggcaagc ttctaacata
      661 ctttcttcat tagctattcc ttttcccgca atatccatag ccgttccatg gtcgggagaa
      721 gtgcggataa atggcaaacc taaagttaca cttaccgttt tataaaaatc taaacacttg
      781 gtggcgatgt gtccttggtc gtgataaaga gataaaacag catcaaattc cccttgaaaa
      841 gttcgccaaa atatagaatc tgctggatag ggccccaaaa tttctattcc cgtatctcgc
      901 accaccgaaa gagccggaat aatctccctt ttttcctcct ctcccaaaag ccccccttct
      961 cctccatggg ggtttagagc tgctactgct atccgggggt gggctaagcc taacctttga
     1021 agataataat gcatccgggg tataaactcg gcaattttat cttttttaat tttctctatg
     1081 gcttctttta aaggaagatg acgagtaaag aaaaaaactc gcaaattttt cacttgaaac
     1141 atagttaaag cttcctcact gtgggtgagc tcctggaaaa tttcagtgtg gccaataaaa
     1201 gggataccag cctcgtggat cgcttctttg tttataggag cagtaactac tccctctccc
     1261 cttccctctt tcattagctc tatggctttc tctatgtatt cataactcgc tcttcccata
     1321 gagggagaaa ttttacccca ggaaaaggaa gacagatcta cattgtccag atcgcaaaga
     1381 aaaggaccca ctcctcggga agaaggaaag tcactccagg gtatctttac tccctgcatt
     1441 tcttcagcct tttgtaatat tttccggtct cctactacta taggaacagc ttctacgtcc
     1501 catttaatta tacttttgac tacgatttcc gggcccaccc cggaggggtc tcccatagta
     1561 agaatcaaag aaagcttgtt cattttcttc ccttcctaaa ctctactaat ctctttactc
     1621 cctcttctac ttcttctctt ttgcgacaga tagaaaggcg gacaaatcct tctcctcctt
     1681 ctccaaaagc atcaccagga gacaaggcta ctttactttc ctttactagg gtcttgcaga
     1741 agtctaaaga cgataaacca aattcctgga tatctatcca cagataatat cccccctgag
     1801 gcttaaagaa gtggaatcct ccctgttcta gaagctctat agctttatct cgattgccct
     1861 ggtaataatc taccatcttt tttacttctt ccttgggtaa atctagagca atttctgctg
     1921 ctttctggga gagatgagaa gtgcaggcaa taagatattc ttctacttta gctaaatgtt
     1981 taactaaata gggaggaaca atcatatacc ctactctcca gccggttaaa gcataagttt
     2041 tagaaaaaga aaaaatactt atcaaattat cttcgtttat aaaggaaaac atcccttggt
     2101 gttctccctc atagacatat ttttcataag cttcatcaga aataagaaga agcccatatt
     2161 cttctgctaa ttgggctaat tggcaaagag tttccttagg aaacaccacc cctaaggggt
     2221 tagaaggaga attaatgagt atcattttag tgcgagaggt aatcagattt tctatctcct
     2281 ttatatcagg taaaaagccg tttttttctt gcaggtgata tctttccatt tttcctccac
     2341 acaactccac ctgataaaaa tggttagtaa aaccaggatc cggtactaaa acttcatctt
     2401 ggggctccaa gagggaggaa aaaatcgtta ctgttccaaa taaagaacca ggaagaatta
     2461 ccacttcttc cggtgttact ttccatcctt tttcttcttt caagtgatgg gcaatttttt
     2521 ctcgcaagct gggtattcct acagtaggag tatacttaat ctctccctgc cagtaaaagt
     2581 ctttcagttt ttctaatata taatgaggag tggaaaaatg aggctctccc atctctaaac
     2641 ggatgacttc cgggtcacgg agagctatct ccattaactt tcttatcccc gagcgaggta
     2701 aattgactat tctttcaggt atatctatca tttggaggtc tccttaaaag ggtttttcgg
     2761 gaacttttgc ccaatcggct aaaaattgct caattccaat atcggtgaga gggtgtttaa
     2821 ttaacttaga tatgacagaa aaaggaatag tggctacgtg agctcccagc ttggctgcct
     2881 ctattacatg catggggtgg cgaatggaag ctacaattac ttgagtagtg atatcgtagt
     2941 tgcagaaaat ttgtaagata tcggctacca ggtgcatacc atcgcttgat atatcatcta
     3001 atctccccac aaaaggactg acataagtag ctcctgcttt agccgccata agggcctgta
     3061 aagggttaaa aattaaagtg gtgtttactt ttaccccttc ttgagaaagg gctttgatag
     3121 cctttaaccc ttccgcagtt ataggaactt tcaccactat attaggcgcc caagtggata
     3181 actctttccc ctcttttatc attccttcgg cttttaagct cactacttca gcagaaactg
     3241 gtccaggaac caagctacaa atctccataa ttacttccct aaagcctctt cccgttttgg
     3301 aaactaaagt aggattagta gtcactccat ctaagattcc cagttttaga gcttctttga
     3361 tttctcccac atcggcagta tcgataaaaa acttcacctt gggcctccct tctctcttga
     3421 tttatcagta ttataagttt cttcttcatc tttatcaatc ttatctatcg ctgcatgtta
     3481 taatctattc aatttagtat tcaaaagaga ggagtctata atatgggaag tgtgaaagac
     3541 ttaatagttc tttcttctcc tcatgaggag gaaccaggaa gaggtaagtt tattttttcc
     3601 gatcgatatt cagtttttga ttggggggag atgcccgatc atattcctct gaaaggtgct
     3661 tctttatgtt taataggagc ctactttttt gaacgattag aagaggccgg gtttctaact
     3721 cattatcaag gggtagagga agagggaaaa ctcaagagat tgtcagattt aaaaagtccc
     3781 actaaccaaa tgatagtctc tttggtaaga gttatccaac ctccagaaat ggaaggaaaa
     3841 tatgattata atatctatca aggagtaaga gggaattttt tgattccctt tgaatttata
     3901 tatcgtaatt ctttacctgc aggctcgagc ttacgcaaaa gattagaaaa aggagagata
     3961 aaattagaag attatggctg ggataaaata cctgctccag aagagagact atcaccccct
     4021 attgtagatg tatctactaa attagaagaa caagatcgtt atttaagctg gagggaagta
     4081 gaagaaacag gaatattaca aaaagaagaa atagaaaaaa ttcgccaggt aattctggct
     4141 atagatgact tgataaccaa agaggtagaa aaaatcaatt tgaagaatga agatgggaaa
     4201 atagaactgg ctttggataa agatagaaat ttagtagtgg tagatgtagt aggcactcct
     4261 gatgagagtc gttttactta ccgagggatg catctttcta aagagattgc ccgggaattt
     4321 tatcggaaaa ctccctggta tgaagaggtt caaaaagcta aacttcagaa tccagtaggt
     4381 tggagaggtt tagttaaaac cagtcctcca cccctaccct cttcgctttt gcaattaata
     4441 agttgggtat acgagcgaat agctatggat cttaccaaca gagattggtt tcccaatgtg
     4501 ccttctttag aagaaattgg ggaaataatt ggccaggaac tccttatttg acagaaaaaa
     4561 agaggaaaag taaaatagtt ccgtaagtgt cgatgtgcgc ccgtagctca aaggatagag
     4621 cggcggtctc ctaaaccgca ggctgagggt tcgaatcccg ccgggcgcac caaggcttca
     4681 gagagcctct tttaagattt tctcactctc tctaaaactc ctgaatccga tgactataaa
     4741 tccattctca gagaggaatt tttcaggccc aaaccaagaa ttaccaacta tatccttgag
     4801 aaaggtattt ctttgccaaa gctaatcgtg ctctaatttc tatttcttca taaggggaaa
     4861 aagctaatct ctccagaaaa tagatggtag caggagcttc tatttcttct aaagctctaa
     4921 ttatttctcg ctgaagggca agagaagttt ctcttgtggc tatctcaaga agaggcagag
     4981 cttcttctcc caaaataggt aatctcctta aaataaataa ttgggctctt tcgctaccag
     5041 agaggagtgt ctctcgaatc aaatacaaat tgcttcttaa atcttcttta ctgagtggag
     5101 gaatctcttc ttcctggaga taaagctctt ccccagaaga tgccaacaaa gaaaaaataa
     5161 tttcagctct ctctccttgg ggaagaaaac gtaaaagaga taaaggtctc cactcccttt
     5221 ctttaagttt ttctttaaga agagaggtaa acgcaatatc ttcaatttca cccaaagatt
     5281 caaaaatttg ttcttccaac atagggtctt tctctcctat ctcgattaga agattcactc
     5341 ccctttcata ttgggttaaa actcttatca ggcgagaatc caagtcttct ccttgtaaaa
     5401 caagatgggg aatttttcct tctccccaga gaatagcttg agagagattg ctaacttttt
     5461 tatcctggtc attggacatt ttgtaagcat aactcataat attattatcc cctctttccc
     5521 atagagtgaa gagggctatt tcccgaaaga aagggttttc cgagtttgtc atagctaata
     5581 aataagaaga tacggagaaa tcgtaaagag aaagcaattc gtccgcttcc tcttttaatt
     5641 caggaggaaa atcttctttc tctaccaagc gaaaaaagaa aggtagagat tcccttacct
     5701 tccaatgggc taaagcccgc agcgcctccc attgtaaaac tacctcttct ttttcgttac
     5761 ttgaatttcc tccctgtaaa atggaaaaga gaaaaggaat ccagtcatct gtctctccta
     5821 aaaggcggat tatttctact cgtatggcta cacttggctc tctctgaaat agagaaatta
     5881 agtactctcc tcgctctttc ccctccattt gtcgagaaag aaaggaaaga gctgatttac
     5941 gaagataaaa gggatattcc ctattttcta ctatttttct tatttcacca gtaagctcgc
     6001 gatgatctaa gaaaagagga gtatcttcaa ttaaaagata agtgatatct tctctcccct
     6061 ctttccagag actgtgtaac tcccgaatta aagagggata agaaagcaat tcttctcctt
     6121 gtactatttc tattcctata aagagaaaga acataattat cactgttagt agatacgctc
     6181 tcgagctcct caggtgtaga ttttcaagtt tttcagcttc ttctaatagt ggaaaagctg
     6241 aagtttcttt aaaaatcatt attttcactt cccttgcatc cgcaggaacg acggatgata
     6301 atttctactt ttaagactac ctctcttttt tcccgaattt cctttttttc aattctttct
     6361 aaaagaaatt gggtagctaa gcttcccata gtataggcag gttgcttggc tacagtgaga
     6421 aaggggtaaa ggagagaggt ggtttctaaa tcatcaaagg taactaaagc taaatcttca
     6481 ggaattttaa tccctctttc tcttgctgct ctaacgatac cgatagctat aaaattgttt
     6541 ccggcaaata ctgcccgagg taactttttc ttttcactga aaaaccgagt agctatttga
     6601 taaccaaaat cttccttgta agagccaacc atgatccatt ctggcttcac cgggattcct
     6661 gcttcctgca aagctctctg gtatccggct accctatctt tgctggtaga gatagttata
     6721 ggaccagtaa tcatagctat ttcccgaaga ttatgaactt ctattaagtg ccgggtcagt
     6781 tcataggctc cttctttact atctcccatt accaaatctc cgtcaaaacc ttctattttt
     6841 ctgtccacca gaactacaga aacttctttt ccttttaatt ttcgtaaagt atccccattg
     6901 ctgcagctgg ctatcaccaa cccgtctact cttcttctta acattaaatc tacatattct
     6961 ttctctttag ctgggttttc atcagtatta caaaaaatag tattaaatcc ctgactagaa
     7021 gctttatctt ccactccccg agctaaagtg gtaaaaaagg ggttagttac atcagagata
     7081 ataagggcaa tagtaaaggt ctgcttcttt tttaagctcc gtgctaagta gttgggagaa
     7141 tagttgagtt cttcagctat ctccaaaatt ttctttctgg tttcgtcttt tacctgactt
     7201 ttctggttta aagctctaga tactgtggct ggtgatactc cagccagctt agctatatcg
     7261 tatatagtag ccattctacc tcctagtaga attactgagg aactttttaa ttttcagcaa
     7321 tttttccata ttatctttta attttaattc tacaccgtaa gtgttatttt tgctttttat
     7381 ataggtaaag gatatcttcc tttttctttc agcaagttta ttaatttatc gtaaatcaaa
     7441 gggtcaatat tactggctac tgagtggtct gaggcgggca tatatccctc tcctcgagca
     7501 gcctgcaatt tataaagggc ctctttttct atttcttccc aggttccata acccaggaga
     7561 cgagtgtcaa ttccacccca aaaagaaaga cgatcgccga actttttctt tagctctgcc
     7621 gggtccatgc cggctttagc ctctaagggg ttataaacat ttatttctgc ctctatcaaa
     7681 ttttccatta tttctaaact tctgccacat ccatgataga caattatcaa acctttttca
     7741 tgtagaaaat cgtaaatcct ctttacacag ggaaagtaaa tatcctgcca cagacgagga
     7801 gaaaaaaaca tccctttatc ataagctacg tctccccaaa caaccattcc cacaggctta
     7861 gccagttcaa tctggcgctc agcaattcct aacatgaagt cagtgcgtct cacggcaaat
     7921 tcttttactt tgccaggaga aagagctaaa tcaagtaaca acccttcggt tccccgaatg
     7981 cgcccatagt ctcatgaggc tcacatatgg agccaaaaat acagaattta ttcttattat
     8041 tctctatagc ttcagcataa ctttctatgg ttccaaaact atctccacaa ttaatgatgc
     8101 cacagcattt ctcaaagtag cggcggtcat ctcgagggtc atcaaaggta aaattggcaa
     8161 actcatcagc acttttcact tcaaaatcta aaaacatagg cattggagca gcaaataact
     8221 tttttacagt gcacccaaaa ccacttttaa aaaccacgta ttctggggtt tcttcaataa
     8281 tttgacaact ctccacctta ggatccatat tgggagaaat gacttttata tctaaatcat
     8341 aatattcata aatatcagtg tcagaaggga ggttaaattt ttctcgccat ctatttaaaa
     8401 attcggtcca gtaaaaatca cctaaaggca ctcgatctgg ttcttttcta tcccggagag
     8461 cagttattcc tcgctcaatt ttcttatcta ctttttccgc cttttttcac tcctttacaa
     8521 atattcacta atagccaact cttctaattt ccctctctca gggaatccat tatctatatt
     8581 ccatccagca aaagaataga aaagttctat agcttctttc aaatcctgag gattaatgcg
     8641 caaactccgg cgaggaccac tctttaccgg ctcaaaaaat ctctctggca accaatcttc
     8701 ctttttttct attccttcct ggagattaaa caatcgttcc agctgcattc tcgtctcccc
     8761 tattttcatc aatcggaaaa ggctcatttc ccagccggtt atagcctgta ttaagggaga
     8821 aatcagagaa aaaggtaaaa atcggcaggg agcaaaagca aatatacaaa gtcccaaagc
     8881 atccataaaa ctaaaaaact gatcgaggta atagaacatt cgcacttttt tagcttccag
     8941 agaaggagcg ggcaatctct ctaaaaatcc taaagcctga atattgtcca aaaaaagttg
     9001 aggagcattt tcatcaaagt cagtatcgtg ttctactaca aaagtatcag gtcctgaggg
     9061 agacacggca taacccagac ctagcatttg ttttactctt ggctcatgaa gaagaaattc
     9121 ctttcctttt gcttctaaag caaaaggaaa ggtttctttc cctagaatat tgctcaggtg
     9181 tttaactcct tttctcactt ccttacaaga taaacaaact gaacaacccc cgtaaccgat
     9241 ttctataata atatttcatt gttattttgt caatccggtt ttaaactatg gaaaatatct
     9301 ttttacagcg tagataaaaa gaaaaaacga agaaaatgtt tcgatataat ttgttttttt
     9361 ggtgttttag tattttaaca ggttttgtag aatactataa aatagtaacg tagcagcaat
     9421 atttatatag ttagccgcaa aatttaaaag caaattagat aaaactataa gaggcagcac
     9481 tcccttattg accccccaaa agaaaaaaca aaaactactg gtgagaaaag ctctaccagt
     9541 atgctgaaga taaaacactc cagtatagcg accttgagtt tttgacttct gacgtaatat
     9601 aatctacttt ttttaaactt tcagcagctt aatcagtttt gcgggatact ataattgcta
     9661 cgacttaaaa taaaaataag agttttgact cagaagaaag ggtttcattg cagaatttca
     9721 taaaatgaag taaaatcaat tcgcaatcga tttcttatta taagataata taaaaggagg
     9781 atttttaatg gctaaagttg gtgttattac tttttccgat ggaagacccc atgttcacca
     9841 agaacttctt tctatgaatc ttcaatttca gaataattta gtta
//
LOCUS       DAOSVE010000067         9737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_3816_length_9737_cov_86.025408, whole genome shotgun sequence.
ACCESSION   DAOSVE010000067 DAOSVE010000000
VERSION     DAOSVE010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 9737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9737
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3816_length_9737_cov_86.025408"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PK016_06310"
     CDS             <1..295
                     /locus_tag="PK016_06310"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HXL02712.1"
                     /translation="SENFNTYQGDTIFLPCSTGFWIVALEADGDKDIWDADLRGMPLG
                     LVVGGEHEGIRKVVCSLCDMVLRVPSRGVINSLNVAVALGIGIYEVMRQRSTN"
     gene            295..1656
                     /gene="rlmD"
                     /locus_tag="PK016_06315"
     CDS             295..1656
                     /gene="rlmD"
                     /locus_tag="PK016_06315"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HXL02713.1"
                     /translation="MDKGQIIEGKVVDFALPEGQGVLKEGGWVIFVPGVLIDEVCRVK
                     ITKLKRNYALGEAIEILEPSPSRMEPLCPHFGEGCGGCRFQFMAYEEQIKVKERNARQ
                     TLEKVGGISWEEVDYEGFTPSLSPWEYRNKMEFNFGEKEGELVLGLRPRGRYWDLIDL
                     KTCYLMKKDLTQALLQLFRDYGKSSSLSGYDPRKKEGVLRNLLVRYSSSQELLVGLST
                     TGIDLPREEDLVGELRKNFPFLEGLIHIINNSPASALIFEDKRILWGKDHFFERVGTI
                     NYKVSLESFFQVNRFLSLALYEKAARYASLNGGETVLDLYCGSGGVGLFVADSAQQVV
                     GVEENPQAVEDAKFNAWLNGRSNFTCVPGRVDKVLPLFSNVGVDVVIIDPPRAGLDKK
                     VVRRVSSLSPQRLVYISCNISTLARDLLLFKEQGYHLGKISFLDLFPQTPYFESVALL
                     KKG"
     gene            complement(1778..2266)
                     /locus_tag="PK016_06320"
     CDS             complement(1778..2266)
                     /locus_tag="PK016_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HXL02714.1"
                     /translation="MKENILIAYYSRSGNTRKLAQLIHQKVGGTVHEIQPEVPYPASY
                     NATVEQAKKEIQAGFRPPLKTKIEHIEAYDTIFVGSPNWWSTIAPPVATFLEETDLSG
                     KTIIPFCTHGGGGRGRIEKDIAKLCPQSTVLPVFAIYGSGGRDAEAQVSAWLKSMGFT
                     EK"
     gene            complement(2287..3273)
                     /locus_tag="PK016_06325"
     CDS             complement(2287..3273)
                     /locus_tag="PK016_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXL02715.1"
                     /translation="MKKRKLGNSGLEVSAIGLGCMGMSQSYPPFPEKGEMISLLRSAV
                     ELGVTFFDTAEVYGPYTNEELVGEALEPYRDKVVIATKFGFHIQNGKSVGLDSRPETI
                     RKAVEGSLKRLRTDHIDLLYQHRVDPNVPIEEVAETVGQLMKEGKVLYWGLSEAGVQT
                     VRRAHAVLPLTAIQSEYSMWYRKPEEELFPALEELGVGFVAFSPLGKGFLTGRFNKDT
                     RFDSTDFRSSVPRFKPENLEANQALLELIKDIAAKKNATPAQIALAWVLAQKPWIVPI
                     PGTKKLERLKENLGAQDIDLSAQELAEINEALSKIKIAGERYPEELEKLTGK"
     gene            3459..4634
                     /locus_tag="PK016_06330"
     CDS             3459..4634
                     /locus_tag="PK016_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009289447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXL02716.1"
                     /translation="MIYKQFQNLSVSHLGMGAMRLPTTDPHGSIDEEKARSVIEYAYE
                     HGVNYFDTAYRYHKGESERFVGKVLSQYPRESWYLATKMPGHMMRYRNGRLEFTGYLS
                     GQSPKSPAQIFEEQLEKCGVDYFDFYLLHNVCETSFDFYTDEEVGVVEYLLEQRKAGR
                     IRHLGFSSHGRPETIDKFLNWKNCFEFVQIQLNYLDWVLQDAKQKYEVITDHGIPVFA
                     MEPCRGGRLASLNEKADAMLKKARPHDSIASWAFRFLQSLPNVGVVLSGMTTMDQVVE
                     NVRLFSYPDPVTEEEEKLLQQVIATMVNLVPCTACRYCCDVCPQELDIPKLISMYNEA
                     SFEPSSTINFTLGAMKDSELPSACIACESCKNLCPQEIDVPRIMKEFNEIIKNISKI"
     gene            4901..7165
                     /locus_tag="PK016_06335"
     CDS             4901..7165
                     /locus_tag="PK016_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HXL02717.1"
                     /translation="MNRDIDKIISQMTLEEKAGLCSGFDAWHTKPVERLNIPSIMMSD
                     GPHGLRKQATESDHLGLHQSVPAICFPSGAGLACSWDRDLLKEVGAALAEECQAEGVS
                     IILGPAANIKRSPLCGRNFEYFSEDPYLSSQMAASHIRGVQSGGVGTSLKHFVANNQE
                     HRRMSVDAIIDERTLREIYLASFEAAVKEARPWTVMCAYNKVNGTYCSQNRYLLTDIL
                     REEWGFDGFVVSDWGAVDERVEGLIAGLELEMPSSGGIRDKKIVEAVKNGKLSEEVLD
                     KAVKRLLSIIFKAVDNKKESVSYDKKAHHALARKVAEQCMVLLKNDDNILPLKESGTI
                     AVIGAFAKHPRYQGGGSSHVNPTEIDAPYEKIKEMSKDIKLLYADGYKLDSDEVDETL
                     LSEAKSIAKQADVAIIFAGLPERYESEGYDRLHMQMPDNHNRLIEAVSAVQSNTIVVL
                     SNGAPVEMPWISSVKGLIEAYLGGQAFGGAIANVLFGRANPCGKLAETFPETLPHNPS
                     YLNFPGEDDRVEYREGLFVGYRYYDAKKIEPLFPFGYGLSYTTFEYTDISVDKKEIND
                     TDTVTVKAKVKNTGKVTGKEIVQLYVRDIEHSVIRPEKELKEFCKVELAPGQEETVEF
                     VLDKRAFAYYNADIKDWHVESGDFEILIGSSSKDIALRETIKVNSTVRIKKQFTRNSL
                     LGDIMADPAAFQVMKELAAQLNIPLGAGDKKSDEIMLAMLKYTPLRALIAFSGGAFTE
                     DMMNMLINKLNEIG"
     gene            7808..8227
                     /locus_tag="PK016_06340"
     CDS             7808..8227
                     /locus_tag="PK016_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HXL02718.1"
                     /translation="MKISEVSEKFGISADTLRYYERIGLLPPANRDGKGIRDYTEEDC
                     EWISFIKCMRAAGLSIKTLIEYVTMFQEGDSTIRERKNLLIEQRKQLAERIEEMKQTL
                     ERLDKKIERYEDRLVKYEERLRSFSDLVASGEGVFSD"
     gene            8976..9113
                     /locus_tag="PK016_06345"
                     /pseudo
     CDS             8976..9113
                     /locus_tag="PK016_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015175441.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            9322..9398
                     /locus_tag="PK016_06350"
     tRNA            9322..9398
                     /locus_tag="PK016_06350"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9356..9358,aa:His,seq:gtg)
BASE COUNT         2834 a         1773 c         2278 g         2852 t
ORIGIN      
        1 ctctgagaac tttaatacct accaggggga taccatcttc cttccgtgtt ccacaggttt
       61 ctggatagtg gctctggaag cagatggaga taaagacatc tgggatgcag acctccgggg
      121 tatgcctctg ggtctcgtgg tgggaggaga acatgaaggg attagaaaag tagtgtgttc
      181 tttatgcgat atggttttga gggtaccatc aaggggggta ataaattctc tcaatgtggc
      241 agtagcttta gggattggaa tttatgaagt tatgagacag aggagcacta attaatggat
      301 aaaggacaga ttatagaagg gaaagtggtt gattttgctc ttcctgaagg acagggggtt
      361 ttaaaagaag ggggctgggt gatatttgtt cccggagttt taatcgatga agtgtgccgg
      421 gtaaaaatta ccaaactgaa gaggaattac gctctgggag aagcgataga aattttagag
      481 ccttctcctt cccgaatgga gccattatgt cctcattttg gagaaggatg cggagggtgc
      541 cggtttcaat ttatggctta tgaggagcaa attaaagtaa aagagagaaa tgctcggcag
      601 actctggaaa aagtaggagg catttcctgg gaagaagtgg attatgaagg ttttacccct
      661 tctctctccc cttgggaata tagaaataag atggaattta attttggaga aaaagaaggg
      721 gagctggttc tgggtttaag accccgaggg cgatattggg acttgataga cctgaaaacc
      781 tgttatttga tgaaaaaaga cttgacgcag gctcttttac agttatttag agattatggg
      841 aaaagctcct ctctttcagg atatgacccc cggaaaaagg agggggtgtt gagaaacttg
      901 ctggtacgct actcctcttc tcaagaactt cttgttggtc tttctactac tggtattgac
      961 cttccccgag aagaagattt ggtaggagaa ttacggaaaa actttccctt tcttgaaggt
     1021 cttatacaca taattaataa ctcaccagcc agtgctttaa tttttgaaga taaaaggatt
     1081 ctatggggca aagaccattt ttttgaacga gtaggaacaa ttaattataa agtttccctg
     1141 gagagttttt tccaggtaaa cagatttctt tctctagctc tttatgaaaa ggccgctcgt
     1201 tatgcttccc taaatggggg agaaacagtt ttagaccttt attgtggaag tgggggagta
     1261 gggctttttg tggctgattc tgctcagcag gtagtgggag tggaagaaaa tcctcaagca
     1321 gtagaagatg ctaaatttaa tgcctggtta aacgggcgga gtaattttac ttgtgtccct
     1381 ggtcgggtag ataaggttct ccctcttttt tccaacgtag gagtggatgt agtcataata
     1441 gacccccctc gagcgggtct ggataagaaa gtggtaagaa gagtttcttc tctttctcct
     1501 cagcgcctgg tttatatttc ttgtaatatc agtactttag ctcgtgactt attgcttttt
     1561 aaagagcagg ggtatcacct ggggaaaatt tcctttttag acctttttcc ccaaactccc
     1621 tattttgagt cagtagctct tttgaagaaa ggataaactt acctttgcct ctctcccttt
     1681 tctatcgtca taggggttat gagaaaaagt ttacaccgaa agattaaaaa gcaaaaacag
     1741 atttatatca acttatatac tgtttcttta gctgtgatta tttttcagta aagcccatac
     1801 ttttcagcca tgcagacact tgtgcttctg catcccttcc tccacttcca taaatggcaa
     1861 aaacaggtag caccgttgat tgaggacaca actttgcaat gtctttttca atccgtcctc
     1921 tccctcctcc accatgggtg caaaagggga tgatagtttt tcccgataaa tcggtttctt
     1981 ccaggaaagt agcaactggt ggagcaattg tgctccacca gttgggagaa cccacaaaaa
     2041 tcgtatcata tgcttcaatg tgctcgattt tagtttttag cgggggtcgg aaaccggcct
     2101 gaatttcctt tttggcctgt tccacagtgg cattgtatga agcaggatag ggaacctcgg
     2161 gctggatttc atgcacagtt ccacccactt tttgatgaat caattgagca agttttcggg
     2221 tattcccgga gcgggaataa taggcgataa gaatattttc tttcatacaa atcttccttt
     2281 ctaaatttat tttccggtca gtttttccag ttcttccggg tagcgttctc cggcaatttt
     2341 aatttttgaa agcgcctcgt tgatttcggc taattcctgg gctgataaat ctatatcttg
     2401 agctcccaga ttttctttca ggcgttccag ctttttggtc ccgggaatag gaacaatcca
     2461 gggtttttgg gcaagcaccc atgccagtgc gatttgagcc ggtgtagcat ttttctttgc
     2521 tgctatatcc tttatcagtt cgagtaaagc ctggtttgcc tcgagatttt ctggtttaaa
     2581 acggggaact gaactgcgaa aatcggtact gtcaaacctg gtatctttat tgaaccggcc
     2641 agttaagaaa cccttaccca ggggactgaa agcaacgaaa ccgactccca gttcttcaag
     2701 agcaggaaat aattcttctt ctggctttct gtaccacata gaatactcac tttgaatagc
     2761 ggtaagtggt agaactgcat gagcgcgacg aacggtctgg acgccggctt cagaaagtcc
     2821 ccagtacaga accttacctt ctttcatcaa ttgtcccact gtttcagcta cttcttcgat
     2881 gggtacattt gggtctacac gatgttgata caacaaatcg atatggtcag ttctcaaacg
     2941 tttcaaagac ccttccactg cctttctgat ggtctccgga cgactatcca atccgaccga
     3001 tttcccgttt tgaatgtgga agccgaattt ggtagcaatt actactttgt cccggtaagg
     3061 ctccagagct tcacccacta gctcttcgtt agtgtaagga ccataaacct cggcagtatc
     3121 aaaaaaagtt acccccagct caacggcaga gcggagcaga gaaatcattt cccctttttc
     3181 aggaaacggt ggatagctct ggctcatacc catgcatcct agaccaattg ctgagacttc
     3241 aagaccacta tttcccagct tacgtttttt catggtaaat cacctcctga tatttccaag
     3301 tttataaact tgctttggca gcctatagtc attctacagt ttaaagttaa ctccatgtca
     3361 agagctttta aaattttacc cttgacatag agttaactct aagtgatagg atgcttaaaa
     3421 agttagcatt gtgtgtaaat cacaggaagg atgattatat gatatataaa cagtttcaaa
     3481 accttagtgt ctcacacctg ggtatgggtg ctatgcgtct acccacaact gatccgcatg
     3541 ggtcaatcga tgaggaaaaa gcaaggagtg ttatcgaata tgcttatgag catggagtaa
     3601 attatttcga tactgcttat cgctaccata agggtgaatc ggagagattc gtcgggaagg
     3661 tactgagcca gtatcctcgc gaaagctggt atctggccac aaaaatgccg ggacatatga
     3721 tgcgatacag gaatggaaga ctggaattta caggatattt gagcggacag tcaccgaaat
     3781 ccccggccca aatttttgag gagcagctcg aaaaatgtgg tgtggattat tttgacttct
     3841 atttgctaca caatgtctgt gaaacttctt ttgacttcta taccgatgag gaagtgggtg
     3901 ttgtggaata tttgctggag caaaggaaag ctggccgtat ccgtcatctg ggtttctcct
     3961 cccacggccg gcctgaaact atagataaat ttttgaactg gaaaaattgt ttcgagttcg
     4021 ttcagattca actcaattat ctggattggg ttcttcaaga cgcaaagcaa aaatatgagg
     4081 tcattacaga ccatggtatt cctgtattcg ctatggagcc atgccgtggt ggaaggttag
     4141 cctcccttaa cgagaaggct gatgcgatgc tcaagaaagc ccgaccccat gattctattg
     4201 catcctgggc ttttcgtttc ctgcaatcct tgcccaatgt aggagttgta ctcagtggta
     4261 tgacaacaat ggatcaggtg gtggaaaatg tgcgactctt ctcttatcca gaccctgtaa
     4321 cggaggaaga agagaaacta ctccagcagg taatcgccac catggtaaat ctcgttccct
     4381 gtactgcatg tcgttactgt tgtgacgtct gtcctcagga actggatatc ccgaaattaa
     4441 tttccatgta taatgaggca agttttgagc cttcatctac gattaacttt actcttggtg
     4501 ctatgaaaga ttcggaactc ccatcagctt gtattgcctg tgaaagctgt aaaaatctat
     4561 gtccgcagga aattgatgtt cccaggatta tgaaagagtt taatgaaatc attaaaaata
     4621 tttctaagat ataacctcta ccagcttttt cgtccattgc aagctattaa cgggcatgaa
     4681 ttgctaatta gaaaggggtg ataaaaatgg cgcaagcacc actgggaaag actggattat
     4741 atatggagca gaatggtttt ggagctttca tcagatatag ttagtcacaa aatttgtagc
     4801 aatgtttctc tcctgatttc ttgctcatct tttgtgtcag ggtctgacca tattcacatg
     4861 ttataataat gttatcttac ataaatagga ggctgacgtt gtgaatagag acattgataa
     4921 gataatttct cagatgacgc ttgaggaaaa ggcgggttta tgttcggggt ttgatgcctg
     4981 gcataccaag cccgtagaaa ggcttaatat tccctccatc atgatgagcg atggcccaca
     5041 tggacttagg aaacaggcta ccgagtcaga ccatctgggc ttacatcaaa gtgtgcccgc
     5101 catatgtttt ccatcagggg cagggcttgc ctgctcatgg gatagagatt tattgaaaga
     5161 agtaggagcg gctctggctg aagaatgcca ggctgaaggt gtttccatta ttctcgggcc
     5221 tgcagctaat ataaagcgct cgcctctttg tggcagaaat tttgagtatt tttctgaaga
     5281 cccgtattta tcgtctcaaa tggcggccag tcatataaga ggtgtgcaga gtgggggagt
     5341 gggtacatca ctcaagcatt ttgttgccaa caaccaggaa cacagaagga tgtcggttga
     5401 tgcgattata gatgaacgga cgctgcggga gatatatttg gcgagctttg aggctgctgt
     5461 taaagaagcc cggccctgga cagttatgtg tgcctataat aaagtcaacg gtacatattg
     5521 ttcccagaac aggtatctcc ttacagatat actgagagag gagtggggat ttgacggatt
     5581 tgtagtgtct gattgggggg cggtggatga acgggtggag ggcctgatag cagggctgga
     5641 gctggagatg ccatccagtg gtggtataag agataagaag atagtggaag ctgtaaaaaa
     5701 tggaaaactg tccgaagaag tacttgataa ggcagtgaaa agacttctta gcattatatt
     5761 caaagccgtg gacaataaga aggaaagcgt atcgtatgat aaaaaagccc atcatgctct
     5821 cgcccggaaa gtggcggaac aatgcatggt acttctcaag aatgacgata atatactgcc
     5881 attgaaagag agcggcacaa tagctgttat aggtgcattt gctaaacatc cgagatatca
     5941 gggtggagga agctctcatg taaatccgac cgagatagat gcaccttatg agaaaataaa
     6001 agaaatgtca aaagatataa agcttctata tgcagatgga tataagctgg atagtgacga
     6061 ggtagacgaa acattactta gtgaggcaaa atcaattgcc aaacaagctg atgtggccat
     6121 tatatttgct ggtttaccgg agcgttatga atcagaaggt tacgatagat tgcatatgca
     6181 gatgccggat aatcataata gacttataga ggctgtgtca gctgtgcaga gcaatactat
     6241 tgtagtactg agtaacggtg cgcctgtgga aatgccctgg attagcagtg taaaaggctt
     6301 gatagaagct tatctgggag gccaggcgtt tggtggggca atagcgaatg tgcttttcgg
     6361 gcgtgctaat ccctgtggta aactggctga gaccttccct gaaacattac ctcataaccc
     6421 ttcgtacctg aattttccag gcgaagatga cagggtggaa tacagggaag gtttgtttgt
     6481 gggttacagg tattatgatg ctaaaaaaat agagccacta tttccttttg gctacggctt
     6541 gagctatacc acttttgaat acacagacat ctcagtagac aaaaaagaga ttaacgatac
     6601 agatacagtt acagtgaagg ccaaagtgaa gaatacagga aaagtcaccg gtaaggaaat
     6661 agtacagctt tatgttagag atatagaaca cagcgttata aggccggaaa aagagcttaa
     6721 agaattctgt aaagtggaac tcgctcccgg gcaggaggag accgttgagt ttgtgctgga
     6781 taaaagggct tttgcttatt acaatgccga tattaaagat tggcatgtag aaagtggaga
     6841 ttttgaaata ctgataggca gttcctctaa ggatatagca ttaagagaaa cgataaaagt
     6901 gaattccact gtacggataa agaaacagtt taccaggaat tcgcttttag gtgatataat
     6961 ggctgacccg gctgcttttc aggtaatgaa agaattagcc gcgcagttga atataccgct
     7021 gggtgctgga gataagaaat cagatgaaat aatgttggcc atgttaaaat atacaccgct
     7081 tagagcgttg atagcgtttt ctgggggtgc atttactgaa gatatgatga acatgctgat
     7141 taataaatta aacgagattg gttaattttt gagtagctca ttctctggaa gttaaaggag
     7201 atattgttga taatctgata aatcgccgga tatgttgctc catattattg gctgtaaact
     7261 gcactatttc ggtaaatagc tgataggggc tccatacccg gcatctcaag ccatcactaa
     7321 ctctccatag aggaataatt ttaatacccg attaaaccag aagattgaac gctaatttta
     7381 atttgaaaat aagttaaatt tttttatata aaaacttgac ttatagttaa ctccaggact
     7441 tatactggaa atggttgcag ccatcttgca tttttagaat tataaaactg tgattggctg
     7501 tactgaatct atagaaagtg aagatttatt agatgaaata tgtaaagtct ggaaacactg
     7561 gcctggcttt gtcctggctc ttgggttctg gtttttgtgt accacagcgg gaattgtacc
     7621 gtggttgttg aacaagggat acagtcgttc ccattataaa aagagctctt gattcaggta
     7681 tcaatttttt cgatgcggcg aatgtgtatt cagatggaac cggcagaaat cgtcattgct
     7741 acagtttctg ctggtgtgtt tgttatatag tggagtggaa actataatat tgtagggagg
     7801 aaaagacatg aagatttcag aagtaagcga aaaatttggt atttcagcgg atacacttcg
     7861 ttattatgag cgcattgggt tgctcccgcc tgcgaaccgg gatgggaagg gaataagaga
     7921 ttatacagag gaagattgtg aatggattag tttcattaaa tgcatgcggg cagctggcct
     7981 ttctatcaaa acattgattg agtacgtaac tatgtttcag gaaggagatt cgacaattag
     8041 ggagaggaaa aatcttttaa tagaacagcg caaacagctt gctgaaagaa ttgaagaaat
     8101 gaaacagacc ctggaacgcc tggataaaaa aattgaaagg tatgaagaca ggcttgtaaa
     8161 atatgaagaa agattaaggt ctttctctga cttggttgct tctggtgaag gagtatttag
     8221 tgattagaga gaaacattca atatcacgaa agtagtaagc aatgtgcttt tgatttaatc
     8281 agcttggttt ttgttgtccg gaaccagttg acactattat cggtttttat taactggagt
     8341 tgaattttaa ttttctctga atttcttcga ttttcctgca tttttcgcct tgactcgaca
     8401 aaaaccggag gatagtttaa gctgtatctg gaacattgtt ttgttgctct gagctgattt
     8461 taccgtataa gtgctttgtc cagaaaagta ggtagtatcc atctttattt agcttttcta
     8521 tcggctacct gggagtaagg cagggtttga ggtaagcttc tccggaagaa ggggtcaact
     8581 ttaagtataa aaggatgaac gacctttaaa ctcttatact cggaatgtta tcggccgtgc
     8641 tctatttgat aaaaaataag ggagaaaaat tatatgaggc aaattctatc ggtttgtggc
     8701 ttgctgggca atgaatgcca tttttttgat acaaaatgcc agggttgtta tgtggttgaa
     8761 ggaaagactt tttgggcaaa ggaatcctct ccagatacca ttctgtcctt tatacaaatg
     8821 tgcagtcatt gattaacaat tataatagtt gtgggcagtg ttctgaactt ccctgtaata
     8881 aatttattga cttaaaagat cctgagattt ccgatgaaca acattacaaa tctatcgatg
     8941 aaagagcttc aaggttaaag gataaggaag gccaaatgag aaaaaaacaa tggtatgaga
     9001 ctctttttga aaactatgca gaaaaatacg ataaggagtg ttttactcag ggaacgatgg
     9061 gcgaatgcga ttttcttgaa aaagaattga atttcgacaa gtcactcaag ttatcaagtc
     9121 tctccgtctt ttctttcttg gataatttct tatatccttt actggcctct cttcgtgcct
     9181 gcatggaaag tctcacttat agcccctccc ttaagaatat tctctcttaa gggaataata
     9241 atttaacttt gatttttagt tgaggcaact cggtctattg attaaaaagg aatataagtg
     9301 taaaatactg taagcttagt ggtgagcgta gttcaaccgg ttagagcact ggactgtgga
     9361 tccaggggtt gggggttcga atcccctcgc tcaccccaga agtgtattta ctgcatctga
     9421 ggctcttgtc tcctctctct aaaagctccg agtccagtaa ctataaatcc actcccagag
     9481 agaggcgatt ttttatgggt gaggtagtaa agcttcctca acctgagggc tgggaaaatt
     9541 tcttagagct attcctgttt gaaaaaaagc cgaaggaaaa tcccccgcac tatttctgat
     9601 tataagttcc atgtctcaaa tttctttata gttaaccgca aaatccatta taaagactat
     9661 aaaggggtaa acccccttat aaccccccaa aagcaaaaga aaagaacaaa gacttaaaaa
     9721 gcaaataaac aaaaact
//
LOCUS       DAOSVE010000068         9455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_3908_length_9455_cov_68.844574, whole genome shotgun sequence.
ACCESSION   DAOSVE010000068 DAOSVE010000000
VERSION     DAOSVE010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 9455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9455
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3908_length_9455_cov_68.844574"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..1188)
                     /locus_tag="PK016_06355"
     CDS             complement(193..1188)
                     /locus_tag="PK016_06355"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HXL02719.1"
                     /translation="MIKKSWILAVIVLISFCTLSIYRLKFQVNYISASENSSFCNNMP
                     TLQVIYSDQFTIYSPYSEEDFRFKGQIHCHTQASDGNNAPQEIVEAYRSLGYQFVAIT
                     DHDSVTKDPEVPGILFISGVEESSFSGHILGIGVKSQTDEIHPDKVIDYIQEKGIAIY
                     AHPNRWSFSNGWSLKELLSVPRARGIEVINPRRWRTNSENKWDALLTRGYQIWGTAGD
                     DCHSITRGINQAWIVVAAPYLSEDNIITALRNGNFYSTEGPDLSVKVYPDRIVACTSA
                     KSTIEWITKGGIKSKVISGVTEDSYFIKGDEGYIRVAIIRDEDEKKALGQPVFIE"
     regulatory      1375..1481
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1529..1792
                     /locus_tag="PK016_06360"
     CDS             1529..1792
                     /locus_tag="PK016_06360"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HXL02720.1"
                     /translation="MVNRAAKISFRKMVYVAIFCALAVVSSGVSFPIGPTKVFPFPHA
                     INVVAGIMIGPWYGGLAAFLVSSISGCIIGLLFVRVFEKSYTC"
     gene            1820..3061
                     /gene="cytX"
                     /locus_tag="PK016_06365"
     CDS             1820..3061
                     /gene="cytX"
                     /locus_tag="PK016_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HXL02721.1"
                     /translation="MSEDIQPVPSQKRNLSGMDFFLLWMGAAIAISEVWAGGMLAPLG
                     FIGGLGAIILGHIIGNTPLALGGVLGSDLGVPAMVSVRVAFGKWGSYLASILNIIQLV
                     GWTAIMIIICAQSVDNITQILAGYTNIKLWMLIAGLVSTFWALVGTPLWKWMQRMGVF
                     SLAILCLFMTYSVLRNVSLVELLSWKGDSSLPWGAGLDLVIAMPISWLPLVSDYSRFA
                     RESRSAFRGTWWGYFLASSWMYTLGLFLALSTGQANPIPIMLALNWGIIALVIVFFST
                     FTTTFLDIYSGAVSFLNLFPRFQEKLITLFFGLGGTILALVFPMDRYESFLYLIGGIF
                     IPLFGVVIFDYFLYRRRRMDIKELYEGKTLDRGALLSWVGGLMVYFVFLYFLPHWGAS
                     LPSFFASGLIYLGTRRKSNDL"
     gene            3051..3857
                     /gene="thiM"
                     /locus_tag="PK016_06370"
     CDS             3051..3857
                     /gene="thiM"
                     /locus_tag="PK016_06370"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HXL02722.1"
                     /translation="MTYEEKCILVFKKIKEEKPLVHHITNLVVINDNANTMLAIGALP
                     VMSYAREEVEEMVALSQALVLNIGTLTPEVIESAILAGKKANELDIPVVLDPVGAGAT
                     QFRTESAWRILQEVKVSILRGNQGEIGNLLGIKAKVRGVEGDTVDIDMSSLAQEASQK
                     FQTVTAITGKVDVVSDGKRVVKIKNGDLLLTKVTGTGCMATSLCAACAAVEKDYLWAA
                     SSALGFLGVCGEIAARHSLGPGSFRVSLFDTFYGIKEEEFKALLSLEVKD"
     gene            3854..4489
                     /gene="thiE"
                     /locus_tag="PK016_06375"
     CDS             3854..4489
                     /gene="thiE"
                     /locus_tag="PK016_06375"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HXL02723.1"
                     /translation="MKFLDLSLYVITDRKIQKKSSEEVIEEAADAGASVIQLREKDLS
                     TRLGVEEAQKAREVTRRKGIPFIINDRIDIALAVDADGVHLGEEDLPLVLARKIAPSL
                     IIGYSVSTVEEARKAEKEGADYLGAGTVFPTSTKKDAGEPIGLERLKEIKKAVHIPVV
                     AIGGINLSNLEEVLATGVDGVAVVSAIVGAESIREATHSFRQKIELFRRKL"
     gene            4486..5490
                     /gene="thiL"
                     /locus_tag="PK016_06380"
     CDS             4486..5490
                     /gene="thiL"
                     /locus_tag="PK016_06380"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HXL02724.1"
                     /translation="MKLKDWGEFGIISYLKDFFPGGGKRVVLGIEDDCAVVKGPRGFY
                     YLLTCDSQVENVHFVFSLFPPFSLGKRVLAVNLSDIAAMGGNPLFALLSWVLRPNIEA
                     EWLKEFARGIKEEAETFGVNIIGGNLSRTASQMVFDLTLVGEVENNCPLLLRRNSRPG
                     DLVLVTGTLGDSRVGLEIALRGSEEEREKYSFLLERFFTPSPRLRVGRLLHSLKERIA
                     VIDISDGFSQDLGHITEESRVGAVVEVDKLPISPALREWSKEKGKDPFLFALSGGEDF
                     ELIFTVPRERGEEIKQFIEKESGVPVTIVGEIVLQGGVSLKRGGKEEKETASGWNHFQ
                     "
     gene            5503..6330
                     /gene="thiD"
                     /locus_tag="PK016_06385"
     CDS             5503..6330
                     /gene="thiD"
                     /locus_tag="PK016_06385"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HXL02725.1"
                     /translation="MKRALTIAGSDSGGGAGIQADLKTFCAFGVYGMSSITSITAQNT
                     KGVEAIFDLPPSLVAKQIECVASDIGVDAAKTGMLSNSSIIEVVAQEIKKFNIEKLVV
                     DPVMVSKSGALLLQRDAVDTLVGGLFPLSFLITPNLPEASFLVGEEIKNVSDMERACV
                     SLKKWGPRYVLLKGGHLPGEEIVDILFDGERFISYPAPRVATKNTHGTGCTYSAAICA
                     LLALGYPITEAVKVAREYMSEVIAHSLNLGKGFGPVNHLAPLFLRGGVNPEEGGKYW"
     gene            6327..7046
                     /locus_tag="PK016_06390"
     CDS             6327..7046
                     /locus_tag="PK016_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HXL02726.1"
                     /translation="MREPRIGTSGWSYPHWEGVFYPPRLPQKKWLPYYSQFFDTVEIN
                     SSFYHLPRATTFSKWRDTTPSEFIFSVKASRYITHIKKMKEVEEPLNKFYEVARELGD
                     KLGPLLFQFPPQLKFNREIFSSFVELLSSDFRYVFEFRNESFFAEEVYEILKEKDICL
                     CFSDTPSFPYQEVVTSSFIYLRLHGHGSLYTDFYSEEELEEWGAKIRFWLGERDVYCY
                     FDNDYEGFAVKNAQRLREILS"
     gene            7096..8226
                     /locus_tag="PK016_06395"
     CDS             7096..8226
                     /locus_tag="PK016_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXL02727.1"
                     /translation="MERKKLGNTGLSISLLGLGGFHLVELPEKEALRIVNLYLDQGGN
                     YLETAESYGYGNSEEKIGKVAQIRRKDFILATKSTQRSKEGITKALEGSLRRLNTSEV
                     DIFFVHGLNRFEELDEIASSQGALEGVEKAKKEGKIRFVAFSNHVTPEVALNALSLYP
                     FDLLMIPLNYFDRLNFPGWEKDVLPQARKKGMGVLAMKALADGFLWQNERQAIRYTLS
                     LGVDSLVMGANTEEYLKRDIEMVENFVPFTPEEMEKIREEAPELGNYVCRQCDKCLPC
                     PLGIDIPGIFLLEGQYDRQMQDGVIRDPAQYALRDRLRFWLGNQDYAQKAYQKLNPQA
                     SACNQCGECEPRCPYKIPIIYKLNLAHEKLTDTRPPSYCRIS"
     assembly_gap    8229..8328
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8344..8736)
                     /locus_tag="PK016_06400"
     CDS             complement(8344..8736)
                     /locus_tag="PK016_06400"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HXL02728.1"
                     /translation="MAQVELIAQRGKIEAHIGTETVLFKSKGAESEVGHWPVEYLLAA
                     LGSCFAGTVFAYAQTKNFPLEKVVSRISGELASSPSRVQKIEMEVELIGNLTYEEKER
                     ILKAGEKGCTVMNTLRGGVEEIVTKLSY"
     gene            8855..>9455
                     /locus_tag="PK016_06405"
     CDS             8855..>9455
                     /locus_tag="PK016_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02729.1"
                     /translation="MAKKWWAILLITLSLLLLSGCAQVELAVKVAGDATIPSARLAVI
                     VNDATVLSQLKNVAVDEYKKLPEEERNFVKIMTKEDAAPYIVAWEWNFPDQEKAREFT
                     QQFLGSTANLSKDGDLVVLEASLEGKELDNLLNQWGGSAVKPFLNSITLLIKAYMPGE
                     ILSYVEGEVVENKVWQQELNLGEVYKNKPTVNIEVISRDK"
BASE COUNT         2614 a         1671 c         2161 g         2909 t
ORIGIN      
        1 ccactgagga agatgctttt tcttcctcag tagtctttgt tctttttatg ttttcaattt
       61 tttatcttac gccgtaggct gcccctactt gtgatgacaa agtggagaaa aggccgtaat
      121 ctgtcgcagt taactataaa agatgcgtta aggatgatag ggccaataga aagatttagg
      181 tttttatacc catcattcaa taaaaacagg ctgtcccaaa gcttttttct cgtcctcatc
      241 tcgaattatt gctaccctta tatagccctc atctccttta ataaagtagc tatcctctgt
      301 aactcctgaa attaccttgc tctttattcc tccttttgta atccactcga ttgtagactt
      361 tgcactagtg cacgccacaa tcctatcagg ataaacctta actgaaaggt caggcccttc
      421 agtagaataa aaattaccat tccgtaaggc ggtaatgata ttatcttcac ttaaataagg
      481 agctgctact actatccacg cttgatttat gcctcgggtt attgaatgac aatcgtctcc
      541 agctgttccc cagatttgat atccccgggt taataaagca tcccatttat tttctgagtt
      601 agttctccat ctcctaggat taatcacctc tattccccgg gctctaggta cggataataa
      661 ctccttcaaa gaccaaccgt tggaaaatga ccaccgatta ggatgagcat aaattgctat
      721 ccccttttct tgaatataat caataacttt gtctgggtga atttcatcag tttggctttt
      781 tactcctatt cctaaaatat gtcctgagaa agaagattct tctactccag aaataaataa
      841 aatccccgga acttcggggt cttttgttac tgaatcgtgg tcggtgatgg ccacgaactg
      901 gtatcccagg gacctgtatg cttccactat ctcttgggga gcattgttcc catctgatgc
      961 ctgagtgtga cagtggattt gacctttaaa tcgaaaatct tcttctgaat agggagaata
     1021 aatagtaaat tggtcagaat aaatcacttg gagagtaggc atattattac agaaagatga
     1081 attttcagaa gcggaaatat aattaacctg gaatttcagc ctgtatatag ataaagtgca
     1141 aaaggagatt aaaacaatta ctgctaaaat ccaactcttt ttgatcaact aaatctccct
     1201 cttttataaa atggtgaagt ttttaattat agtccatagt tcatcttaat aatactcaaa
     1261 atgaatttaa tttcttagtt atattgttca ggccaggatt tcctattacc gagaagataa
     1321 acaatcttct cccatacgga aagttttacg agtatgatac aatgatgaaa actaaaatag
     1381 aaattgggga gctcgcaaag agctgagagg agagtgtggc tctcgaccca ttgaacctga
     1441 tctggttaat accagcggag ggatagaaat aataaaaagc tacctactgg ctggtaggta
     1501 gcttttttta ataggggaga aaacgaaggt ggtgaataga gcagctaaaa tttcttttcg
     1561 aaagatggtt tatgttgcta ttttttgtgc tttagcggtt gtttcttccg gtgttagttt
     1621 ccccattggc ccgactaaag tttttccctt tccacacgca ataaacgtgg tagccgggat
     1681 tatgattggt ccttggtatg gagggttagc tgcttttttg gtaagtagta tctctggatg
     1741 tattatcggc cttttatttg ttcgggtttt tgagaaaagc tatacctgtt gatacctggg
     1801 aggagatagg aggatagcga tgtcagaaga tatccaacct gttccttcac agaagagaaa
     1861 tttatcggga atggattttt ttctcctgtg gatgggagca gctattgcta tatctgaagt
     1921 gtgggcagga ggaatgttag ctcctttagg cttcatcgga gggctgggag ctattatctt
     1981 gggtcacata attggcaaca ctcctctggc tttaggagga gtgttgggta gcgacttggg
     2041 agttccagct atggtttcag tgagagtagc ttttgggaag tggggctctt atctggcctc
     2101 aattttgaat atcattcaat tagtgggctg gacggcgatt atgataatca tctgcgctca
     2161 atcagtggat aacatcaccc agattttggc aggatacacc aatatcaagc tctggatgct
     2221 tatcgccgga ttggtgagca ctttttgggc tttggtagga actcctcttt ggaaatggat
     2281 gcagagaatg ggagtttttt ctttagctat tttgtgtctg tttatgactt acagtgtttt
     2341 acgtaatgta tctttagttg agcttctaag ttggaaggga gacagttctc ttccctgggg
     2401 agcaggtttg gatctggtga tagctatgcc catttcttgg cttcctttgg tttcagacta
     2461 ctctcgtttt gctcgagaga gccgttctgc ttttcgggga acctggtggg gatatttttt
     2521 agccagttcc tggatgtata ctctgggatt atttttggct ctctctactg gtcaggctaa
     2581 cccgataccg ataatgttgg ccctaaattg gggaatcatt gctctcgtaa ttgttttttt
     2641 ctccactttt accactacct ttctggatat ttactctgga gcggtatctt ttttgaatct
     2701 ttttcctcgt tttcaggaga aactaattac tcttttcttc ggattaggag gcacgatttt
     2761 agctttagtt tttcctatgg atagatacga aagttttctt tatctcatcg gaggaatttt
     2821 tatccctctt tttggggtgg taatttttga ttacttcctt taccgccgca gacgtatgga
     2881 tataaaagaa ctgtatgaag ggaaaactct cgatagagga gctcttctct cctgggtagg
     2941 aggattaatg gtgtattttg tttttcttta ttttttgcct cactggggtg cttctctacc
     3001 cagttttttt gcctcaggtt taatttattt agggacaagg aggaaatcca atgacttatg
     3061 aagaaaaatg catccttgtt tttaaaaaaa taaaagagga aaaacctctg gttcatcata
     3121 tcactaattt ggtggttatt aatgacaatg ctaatactat gttagccata ggagcgctgc
     3181 cggttatgag ttacgcccgg gaggaagtag aagaaatggt agctttatct caagctctgg
     3241 ttttgaatat cggaactcta accccagagg tgatagaaag tgcgatttta gcaggtaaaa
     3301 aagctaatga gttagatatc ccggtggttt tagacccggt gggagcaggg gctactcaat
     3361 tccgtaccga aagtgcttgg aggattttgc aagaagttaa agtctccatt ttgcggggaa
     3421 atcagggaga aatagggaat ctactgggaa taaaagctaa agttagaggc gtggaaggag
     3481 atacggtaga tatagatatg agtagtttag cgcaggaagc cagccagaaa ttccagactg
     3541 ttacggccat aactggaaaa gttgacgtgg taagtgatgg aaaaagagtg gtgaagataa
     3601 aaaatggaga ccttctcctc accaaagtta ccgggactgg ctgtatggct acctctcttt
     3661 gtgctgcttg tgcggcagtc gagaaagact acctatgggc agcatcttcg gctttaggct
     3721 ttttgggagt ttgtggagaa attgctgctc gtcactcttt aggtccgggt agttttcgag
     3781 tttctctttt tgataccttt tatggtataa aggaagaaga gttcaaagcg ttactttctt
     3841 tggaggtgaa agattgaaat tcttagattt atctctctat gtgattactg atagaaaaat
     3901 tcaaaagaaa agtagcgagg aagtaataga ggaagccgca gatgccggag cttcggttat
     3961 tcagctgcga gaaaaagacc tttctactcg tctgggggta gaagaagccc aaaaagcgag
     4021 agaagttact cgccgcaagg gaattccttt catcatcaat gaccgtatag atattgcttt
     4081 agcagtagat gctgatggag tgcacttagg agaagaagat ctgcctttgg ttttagctcg
     4141 aaaaattgct ccttctctta taattggcta ttcagtttct acggtagaag aagcccgaaa
     4201 agcggaaaaa gaaggggcgg attatttggg agcaggaacg gtttttccca cttctactaa
     4261 aaaagatgct ggggagccca ttggtttaga aagattgaaa gaaattaaaa aagcagtgca
     4321 tataccggtg gtggccatcg gaggcataaa tctctccaac ttagaagaag ttttagccac
     4381 cggtgtggat ggagtggcag tagtatcggc gattgtagga gccgagtcaa tcagggaagc
     4441 cactcattct tttcgacaaa aaattgagct ttttcggaga aagctatgaa acttaaagat
     4501 tggggagaat ttggaattat cagttatctg aaagactttt ttcctggcgg ggggaaaaga
     4561 gtagttttag gaattgaaga tgattgtgcg gtggtaaaag gccctcgggg tttttactat
     4621 cttcttactt gtgattctca ggtagaaaat gtgcactttg ttttctctct ttttcctccc
     4681 ttctctttgg ggaaaagggt tctagcagtg aatttgagcg atattgctgc tatgggaggt
     4741 aacccccttt ttgctcttct ttcctgggtg ttgaggccaa acatagaagc ggagtggctg
     4801 aaagagtttg cccggggaat caaagaagaa gcagaaactt tcggagtaaa tataatagga
     4861 gggaatttat ctcgcactgc cagtcagatg gtttttgatt taactttggt gggagaggta
     4921 gaaaataatt gtcctctcct tttgcgccgc aactcccgtc ctggagattt agttttagtt
     4981 actgggacat tgggcgattc ccgggtaggg ctggagatag ctctccgggg tagcgaagag
     5041 gaaagagaaa aatattcttt tcttttggaa cgttttttta ctccctctcc tcgcttacgg
     5101 gtagggaggt tattgcatag tttgaaagaa agaatagctg taatcgatat ctctgatggg
     5161 ttttctcaag acctcggaca tattacggaa gaaagtagag taggggcagt ggtagaggta
     5221 gataaattgc ctatttctcc tgctcttcga gagtggagta aagaaaaagg gaaagatcct
     5281 tttctttttg ctctttcggg aggagaagat ttcgagctga tttttaccgt tcctcgagaa
     5341 cggggagaag aaattaaaca atttatagaa aaagaaagtg gagttccggt aactattgtg
     5401 ggagaaattg tcttgcaagg aggagtttct cttaaaagag ggggaaagga agagaaagaa
     5461 accgcctcag gctggaatca ttttcaataa aggagaaaaa cgatgaaaag agcattaact
     5521 atagccggtt cagattcggg aggaggagca ggaatccagg ctgacctcaa aactttctgt
     5581 gcttttgggg tgtatggtat gtcttccatc acctctatta ctgctcagaa tactaaagga
     5641 gtggaagcca tttttgactt acctccttct ttagtagcca aacaaataga gtgtgtggcc
     5701 agcgatatag gtgtagatgc ggctaaaact ggtatgcttt ctaattcttc tatcattgaa
     5761 gtagtggctc aagagataaa aaagtttaat atagaaaaat tggtggtaga cccggtgatg
     5821 gtatctaaaa gtggagcttt gcttctccag cgagatgctg tagatacgct tgtgggggga
     5881 cttttccctc tttctttttt gattactccc aatcttcccg aagcttcctt tttagtgggg
     5941 gaggagataa aaaatgtttc tgatatggag agagcctgtg tatccctcaa gaaatggggg
     6001 cctcgctatg ttctcctaaa aggaggacac ctcccgggag aggagatagt ggatatcctt
     6061 tttgatgggg aaagattcat ttcttatcct gctccccgcg ttgctaccaa aaacactcac
     6121 gggacgggtt gcacttattc agcagctata tgtgctcttt tggctttagg atatccgatt
     6181 actgaagcag taaaagtagc tcgagagtat atgagcgagg taatcgctca ctccttaaac
     6241 ctgggtaaag gatttggccc ggttaaccat ttagctcctc ttttcctcag agggggtgtg
     6301 aatccagaag aaggaggaaa atactggtga gagagccccg gatagggact tccggttggt
     6361 cttaccctca ttgggaggga gtattttatc ctcctcgttt accgcaaaag aagtggcttc
     6421 cctactattc ccagtttttt gatacggtgg agattaattc ctctttttat caccttcctc
     6481 gtgctactac tttttctaaa tggagagaca ccactccttc agaatttatt ttttctgtga
     6541 aagccagtcg ctacatcacc catataaaga aaatgaaaga agtagaagaa cctctaaaca
     6601 aattttatga ggtggcaaga gagttaggag ataaattagg ccctcttctt tttcaatttc
     6661 cccctcaact taagtttaat agagaaattt tttcctcttt tgtggaactt ctctcttccg
     6721 attttcgcta tgtttttgag tttcgcaatg agagtttttt tgctgaagaa gtttatgaaa
     6781 ttcttaaaga aaaagatatc tgtctctgtt tttctgacac tccttctttt ccttatcagg
     6841 aagttgtaac ttcttctttt atctatcttc gacttcatgg gcacggtagt ctgtacaccg
     6901 atttttacag tgaagaagaa ctggaagagt ggggagctaa aattcgtttc tggctcgggg
     6961 agcgagatgt ttattgttac ttcgataatg attatgaggg ctttgccgta aaaaacgctc
     7021 agcgtctcag ggaaattttg tcttgataag tgtagaattt tcacttttgt gagataattt
     7081 gagggggtga agaatatgga aagaaaaaaa ctgggaaaca cgggtctttc tatttctctt
     7141 ctgggattag gcggttttca tttagtggag ctaccagaga aggaagcact gcggattgtc
     7201 aatttatatt tagaccaagg gggaaattat ttagaaaccg ccgagagcta cgggtatggc
     7261 aactcagaag aaaaaatagg taaagtagct caaattcgga ggaaggattt tatcctagct
     7321 actaaatcca cgcagaggag caaagaaggc ataaccaaag ctttagaggg aagtttaagg
     7381 agactcaata ccagtgaggt agatatattt tttgttcatg gtttaaaccg gtttgaagaa
     7441 ctggatgaaa tagcctcttc gcaaggggct ttagagggag tagaaaaagc taaaaaagaa
     7501 gggaaaatcc gttttgtagc tttttccaat catgttaccc ctgaagtagc actcaatgct
     7561 ctttctcttt acccctttga ccttttaatg attcccttaa attattttga ccggctcaat
     7621 tttcccggct gggagaaaga cgttttacct caagctcgga aaaaggggat gggggttcta
     7681 gctatgaaag ctctggctga tggtttctta tggcagaatg agcggcaggc tataagatat
     7741 accttgagtc tcggagtaga tagtttagtt atgggagcta acacggagga gtatctaaaa
     7801 agagatatag aaatggtgga gaattttgtt ccttttaccc cggaagaaat ggaaaaaatt
     7861 cgagaggaag ctccggaatt aggtaactat gtttgtaggc agtgtgataa atgtctaccc
     7921 tgtcccttag gtatcgatat tcctggaatt tttcttctgg aaggacaata tgaccgacag
     7981 atgcaagatg gcgtaattcg cgaccctgct caatatgctc tgcgagaccg tctccgcttc
     8041 tggctgggaa atcaggatta cgcccaaaaa gcgtatcaga aattaaatcc ccaggcttca
     8101 gcatgtaacc aatgtggaga gtgcgagcct cgctgtccct ataaaatacc cattatttat
     8161 aaattaaact tagctcacga gaaattaact gacactcgtc cacccagcta ttgccgtata
     8221 tcataatann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa ttttacaggg
     8341 aggttagtag gataacttag ttactatttc ttctacgcct ccccgcaaag tattcatcac
     8401 tgtgcagcct ttttcccctg cttttaggat tctttctttt tcttcataag ttagattacc
     8461 aattaactct acttccattt ctattttttg cactcgagaa ggtgaagaag ccaattctcc
     8521 gctaatccga gagactactt tctccagagg aaaattttta gtttgggcat aagcaaatac
     8581 tgttccagca aagcaacttc ctaaagcggc taaaagatat tctaccggcc agtgtcctac
     8641 ttcgctttct gcgcctttac ttttaaataa aaccgtttct gttcctatat gagcttcgat
     8701 ttttcctctt tgagctataa gctctacttg agccattatt ttctacctcc tcgatcggtt
     8761 ttgagattaa agtctagtat aattatacaa tggaactcta ttttaagaag taaatacact
     8821 tttatatagt gaaaaatgaa aaggaggcta aatcatggcc aaaaagtggt gggcgatttt
     8881 gttgataact ttatctcttt tgctcctttc tggttgtgct caagtggaat tagcagttaa
     8941 ggtagcaggg gatgccacta ttcctagtgc tcgccttgca gttatagtaa acgatgccac
     9001 agttttgagt caactaaaaa atgtggctgt ggatgaatac aaaaagttac ccgaggaaga
     9061 aagaaacttt gtaaaaataa tgactaaaga agatgctgct ccctatatag tagcctggga
     9121 atggaatttc cctgaccagg aaaaagctcg ggagtttact caacaatttt taggtagtac
     9181 tgctaatctt tctaaagatg gagacctagt agtcttggag gccagcttag aagggaaaga
     9241 attggataac ctcttgaatc agtggggagg gtctgccgta aagccttttt tgaattctat
     9301 tacccttttg attaaagctt atatgcctgg cgagatacta agttatgtag agggagaagt
     9361 ggtagaaaat aaagtttggc aacaggagtt aaatttagga gaggtatata aaaataaacc
     9421 tacggtaaac attgaggtaa tatcacgaga taaat
//
LOCUS       DAOSVE010000069         9433 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_3923_length_9433_cov_72.189273, whole genome shotgun sequence.
ACCESSION   DAOSVE010000069 DAOSVE010000000
VERSION     DAOSVE010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 9433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9433
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3923_length_9433_cov_72.189273"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..185
                     /locus_tag="PK016_06410"
     tRNA            110..185
                     /locus_tag="PK016_06410"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:144..146,aa:Met,seq:cat)
     gene            287..1567
                     /gene="tilS"
                     /locus_tag="PK016_06415"
     CDS             287..1567
                     /gene="tilS"
                     /locus_tag="PK016_06415"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HXL02730.1"
                     /translation="MAFSGGPDSTALSQILFSLREIIGFEMALFHLNHGLRGEEAKRD
                     EEFCISWARDKGIPIFVKRQDVKSRKATEGLSLEEAARQVRYQVMREVAQTWGADKIV
                     LGHTMNDQGETILLNILRGTGLQGLQGMKFKEDIFIRPLLGIKREEILKFLKEEDISF
                     VEDSSNRDVSFLRNRLRNLIPFLEKEFNPQLIEALFRLSLNVQREEYSEPEVTIPIER
                     KGMLTRAPLSYFKGISSLELLKVVRFFIKEARGNLWDISRGQFEEVAHLLEKGRGEVQ
                     LPGKFKVFIRDGYLWASPDLFFLADVPHWLFPLQLPGENFFPDLGIAIRGNWKGEEKK
                     RMLHSIDWDFEKCYPPFVVRNFQDGDRIWQKGKEIKVKELFPRKGIVREWRKLIPFIC
                     DKEKILWIPGLALDERIRVRENSKQILAVSIGWL"
     gene            1579..2133
                     /gene="hpt"
                     /locus_tag="PK016_06420"
     CDS             1579..2133
                     /gene="hpt"
                     /locus_tag="PK016_06420"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HXL02731.1"
                     /translation="MESFIEQVLISEEEIQAKVKELGERISKDYQGKELRVIGILRGA
                     FIFMADLVRNIKLPLSVDFMSISSYEDDYQTTGVVRILKDLDKPITNRHVLIIEDIVD
                     TGLTLKHLKEVLSTRSPASLKVCTLLNKKEKHVVKDLVIDYCGFDIPNYFVVGYGLDF
                     AEKYRNYPFIFVLKPEYYQKPSEV"
     gene            2152..4038
                     /gene="ftsH"
                     /locus_tag="PK016_06425"
     CDS             2152..4038
                     /gene="ftsH"
                     /locus_tag="PK016_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HXL02732.1"
                     /translation="MNNYPSGRLYRNLGFYLLFLIIMLSVATSFMEREIPPQVFTYTQ
                     FLDAVQKGLVEKVVIMNHDLAGEMKDGTKFTTYAPDDPNLITTLRSKNVDIEARPPAE
                     PSWWVRILGSLLPTVFLIIVWIFLLQQVQGGGNRVTSFAKSRAKMVEPEKVKVTFNDV
                     AGIEEVKEELREVVDFLRNPRRFQKIGAHIPRGVLLYGPPGTGKTLLAKAVAGEAGVP
                     FFSISGSDFVEMFVGVGAARVRDLFNTAKKNAPCIVFVDELDAVGRHRGAGLGGGHDE
                     REQTLNQLLVEMDGFDPNEGVIIVAATNRPDILDPALLRPGRFDRRVAVPPPDVVGRE
                     AILRVHVKGKPIDKDVDLEVLARRTPGFVGADLANLVNEAALLAARKGRETIKMEDFE
                     EAIDKVIAGPERKHMVISEKEKQIVAYHEAGHALVAGALPNADPVHKISIIPRGNMAL
                     GYTLQLPTEDRYLLSKQELLDRLAVLLGGRVAEELVFKDATTGAQDDLKKATDIAREM
                     VCEYGMSEILGPLTLGRKHKEVFLGRDIAEDRNYSEEIAYTIDKEVRAIIDQAYEKAQ
                     EIINRYREKLDLLAQRLVEKEVLEREEIEQILDFNEENESSTLPLQTQINPDLAGGEK
                     TDEI"
     gene            4028..4450
                     /locus_tag="PK016_06430"
     CDS             4028..4450
                     /locus_tag="PK016_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HXL02733.1"
                     /translation="MKFNTLRIGLTGVAQAVVNDENAADHFAPDMVPAFATPMLLSLM
                     DNAAHEAVKNHLPEGYVSVGTRLSISHVAATPKGMQVTARATLVEIHRNRLIFTAEAF
                     DEVEKVGEGRLERFVINRDSFLKKLESKLNFIKGLVTE"
     gene            4465..5151
                     /locus_tag="PK016_06435"
     CDS             4465..5151
                     /locus_tag="PK016_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HXL02734.1"
                     /translation="MRQFTVFGLGIFGSSIAIALYEQGFTVLGVDIEEEPVKELAGKI
                     TEVVQADTTDEKVLEALGIKNFDVAIVSIGNDIQSSVLTTLAVKELGIPLVIARAINE
                     MHGKILHKIGADRVIFPEQDTAIRVAKTLAFPYAIRSEELFPGHNIIESQVPPPLYGL
                     TLTRSQLRTRYGITVIAIKRGENYIVSPNATETLSKGDIIYILGSEQQLKKFFREMAE
                     LRNGKVKVEE"
     gene            5148..6422
                     /locus_tag="PK016_06440"
     CDS             5148..6422
                     /locus_tag="PK016_06440"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HXL02735.1"
                     /translation="MKAQPECFCCSIRQAQEAAEIAGKDKDTIWKVSQEVCAVYAQAN
                     KEWTPAYMTTLAHDVAKKIVGIEDIFYQLKRDHNQAALRFYPQLKEWVRKGDNRLERA
                     VKVAIAGNLIDLGVYRNVKVEEVLEEIDHARWGRYEFSSWAKDLENTHTLIYVGDNAG
                     EVVFDRILLEEISPGRKIFFVVKGGPISNDALLEDAQEAGIGEVAEILTTGQAEVGIA
                     LQRAPSSLQKLWKEADMIISKGQGNFETLSGEEGNIYFLLKAKCIPVARELGVEQGDL
                     ILQKRKASPPEVEVYSSGGLVVRKNDSQWQVLLVKKNDSGLWTLPKGHKEKGEREEET
                     ACREVEEETGYKVKLGPRVGEISFSYVRNYQPFREIASFFLMEVAGEGEKEEEGEIAE
                     VKWFPISEALRVLNYDNERSLVGKALELLERE"
     gene            6582..6842
                     /locus_tag="PK016_06445"
     CDS             6582..6842
                     /locus_tag="PK016_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HXL02736.1"
                     /translation="MELLKISSKTRPVSLAGALTGVIREQGRAEMQAVGAGAVNQAVK
                     AIAIARGYLAPSGIDLVCIPAFVDVKIDDTEKTAIRFIVLPA"
     gene            6883..7173
                     /locus_tag="PK016_06450"
     CDS             6883..7173
                     /locus_tag="PK016_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006351250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HXL02737.1"
                     /translation="MNKQELVNTLSEELKKEGVNVTKKDTAKIVDTFLGVIQEEIKRG
                     GKVQLVGFGTFGVKTRAARRGRNPQTGKEIRIPEATIPFFRPGKGLKELVKE"
     gene            7180..8046
                     /gene="hisJ"
                     /locus_tag="PK016_06455"
     CDS             7180..8046
                     /gene="hisJ"
                     /locus_tag="PK016_06455"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HXL02738.1"
                     /translation="MPNKKEGQFVPPFCFIGREMFLGDYHLHTPRCGDAKGSYDEYIE
                     EALRKGLSEIGFSGHCPQYFLSPEERKRESAIPEEELALYIEEVESLQEKYRSSITIR
                     LGLEVDFIPGKEEELHPLVDFYSWDYLLLSVHYLGDWAFDNPRYLSRYKERNINEIYE
                     SYYQTLIAGMKTGFFSAVAHFDLPKKFGFRPTGEIRAEVEALRVCQEQNLVLELNTAG
                     RRKPVGEFYPAPSILSQAQKLGLQVCLGSDAHQPEDVGQDFKEALALLRDSGFDHIVG
                     WEKRSAVLHPIS"
     gene            8481..8867
                     /locus_tag="PK016_06460"
     CDS             8481..8867
                     /locus_tag="PK016_06460"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HXL02739.1"
                     /translation="MINWRFPSEYRSRTVERCASSLPKIHSEDEALFLAEKLGIEVVF
                     VHLEEAKGGLLWRGKEAYIVINSKFPPTERAKTILHEIAHFLLHHANYLRPVFLDHKS
                     FIAGLEKEADYFAFLLMREWLEEESL"
     gene            complement(8904..9206)
                     /locus_tag="PK016_06465"
     CDS             complement(8904..9206)
                     /locus_tag="PK016_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02740.1"
                     /translation="MGEVVRLHRSEGFHNFLELFLFEKKAQGKAPRTIYDYKFHVSAF
                     FKRFPQALRSDKELRTSVLEYFSDDIKPATFNIRRVYLKAFFDLSSSVSSITLTIT"
     gene            complement(9305..9379)
                     /locus_tag="PK016_06470"
     tRNA            complement(9305..9379)
                     /locus_tag="PK016_06470"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9344..9346),aa:Glu,seq:ctc)
BASE COUNT         2853 a         1583 c         2306 g         2691 t
ORIGIN      
        1 taatggattt tgcggttaac tataattaaa gcatttggga atagatttta aatgattctg
       61 ctggcattct tgtcttttgt aggaaaacag ttataattat tcagtggatg gcggtgtagc
      121 tcagatggcc agagcatccg gctcataccc ggagcgccag ggttcgaatc cccgcaccgc
      181 caccagcagc caaattcgag tttaacgacg agtgcattct ttaataagta aggttaaaaa
      241 agttattgtt gagtattccc ttatttctcc tggagataaa gtattagtgg ctttctctgg
      301 aggtcctgat tctaccgctc tctctcaaat tcttttttct ctccgggaaa ttattggctt
      361 tgaaatggct ttgtttcacc tcaatcacgg tttgcgagga gaagaagcaa agcgtgatga
      421 ggaattttgt atctcctggg cccgagataa gggaatacct atttttgtaa agaggcaaga
      481 tgtaaagagc agaaaggcta cagagggcct ctctttagag gaagctgctc ggcaagttcg
      541 ctatcaggta atgagggaag tggctcaaac ctggggagca gacaaaattg ttttaggaca
      601 tactatgaat gaccagggag aaaccattct tcttaacatc cttcgaggta caggtctcca
      661 gggtttgcag ggtatgaaat ttaaagaaga tatttttatt cgtcctttac tgggaataaa
      721 gagagaggaa atattaaagt ttcttaagga ggaggatatt tcttttgttg aagacagttc
      781 taatcgggat gtttctttct tgaggaatcg gttgcgaaat ttaattcctt ttttagagaa
      841 agaatttaat cctcagctta tcgaagctct gtttcgttta tctcttaacg ttcaaagaga
      901 agaatattca gagcctgagg taactatacc aatagagaga aaaggaatgc tgacaagagc
      961 ccccctttct tattttaaag gtatttcctc tttagaatta cttaaggtgg ttcgcttctt
     1021 tattaaagaa gctcgaggta acctgtggga tataagtcgg gggcaatttg aggaagtggc
     1081 tcatttgctg gaaaaaggaa ggggagaagt acagttgccg gggaaattca aggtatttat
     1141 aagggatggg tatctctggg cttcccctga tttgttcttc ttggctgatg ttcctcattg
     1201 gctttttcct cttcaacttc ccggtgagaa cttttttcct gacttaggaa tagctattag
     1261 agggaattgg aagggggaag aaaagaaaag gatgctccac tctatagact gggattttga
     1321 aaaatgttat cctccttttg tagtgcgaaa ctttcaggat ggagaccgaa tctggcagaa
     1381 aggaaaggag ataaaagtta aagaactttt ccctcggaag ggaatagtaa gagagtggag
     1441 gaaattaatc cccttcatct gtgataagga aaagatactc tggattcccg gtttagctct
     1501 tgacgaaaga ataagagtta gagaaaatag caagcagatt ttagcagtgt ctattggctg
     1561 gttgtgagga gtggtagagt ggaatctttt attgaacaag ttttaatttc agaagaggaa
     1621 atacaggcta aagtaaaaga gctgggagag agaatcagca aagattacca gggtaaggaa
     1681 ttaagagtga taggtatttt acgaggcgct tttattttta tggctgattt ggtgcggaat
     1741 attaagctcc ctttaagtgt tgactttatg tcgatttcaa gctatgagga tgattaccaa
     1801 accactggag tggtgagaat acttaaagat ttagataaac ctattactaa tcgtcatgtt
     1861 ttaataatag aagatattgt ggatactggc ctgaccctaa aacatcttaa agaggtgcta
     1921 tctactcgta gtccggccag ccttaaagta tgtactttat taaataaaaa ggagaaacat
     1981 gtggtaaaag atttagttat agattattgc ggttttgata tacccaatta ttttgtagtg
     2041 ggctatggtc ttgattttgc agaaaagtac cgtaattatc cttttatatt tgttttaaaa
     2101 ccagaatatt atcaaaaacc gtcggaagta tgatttagtg ggagagtgac aatgaacaat
     2161 taccctagtg gaagattata tagaaatctg gggttttatc tcttgtttct tattattatg
     2221 ctctcagtag ctacttcttt tatggaaaga gagattcctc cccaggtgtt tacctatacc
     2281 cagtttttag atgcagtgca gaagggtctg gtggaaaaag tagtaattat gaatcatgat
     2341 ttagctggag agatgaagga tggtactaaa tttaccacct atgctcctga tgaccctaat
     2401 ttaattacta ccttgcgcag taaaaatgtg gatatcgaag cccgaccacc agcagaaccc
     2461 tcttggtggg taagaatatt aggttctctt cttcctaccg ttttcctcat catagtgtgg
     2521 atttttttac tccagcaggt acagggagga ggaaatcggg ttacatcatt tgctaaaagt
     2581 agagctaaaa tggttgaacc agaaaaggta aaagttactt ttaatgatgt agccgggatt
     2641 gaggaagtga aggaagaatt aagggaagta gtagattttt tacgtaatcc ccgtcgtttc
     2701 cagaaaattg gagctcatat accccgggga gttttactct atggacctcc tggtaccggt
     2761 aaaactttat tagctaaagc cgtcgctggt gaggcggggg ttcccttttt cagtatcagt
     2821 ggttcagatt ttgtggaaat gtttgtggga gtgggagctg ctcgagtgag agacctcttc
     2881 aacactgcca aaaaaaatgc tccttgtata gtttttgtag atgaattaga tgcagtggga
     2941 agacatagag gagcaggttt aggtggtggt cacgatgagc gagaacaaac cttgaaccaa
     3001 ctcctcgtgg aaatggatgg ttttgaccct aacgaggggg ttattatagt tgctgctact
     3061 aaccgtcctg atattttaga ccctgctctt ttgcggccgg gaagatttga ccgtcgtgtt
     3121 gctgttcctc ctcctgacgt ggtgggaaga gaagctatct tgcgggttca tgtaaaaggg
     3181 aaacctatag ataaagatgt agatttggaa gttttggctc gtcggactcc tggctttgta
     3241 ggggcggatt tggctaattt agttaacgaa gctgctcttc tcgctgctcg aaaaggcaga
     3301 gagactatca agatggagga ctttgaagaa gctattgata aagttatagc cggtccagag
     3361 agaaaacaca tggttatcag tgaaaaagag aagcaaattg tagcttatca cgaggccggt
     3421 cacgctctcg tagccggggc tttgcctaat gctgacccgg tacacaaaat ttctattatt
     3481 ccccggggaa atatggcttt aggttacact ttacagcttc ccactgaaga ccgttatctt
     3541 ctcagtaaac aagagctatt ggaccgtctg gcggttttat taggtggcag agtagcagaa
     3601 gagttagttt tcaaagatgc cacgactggt gctcaagatg atctgaaaaa agcaacggac
     3661 attgctcgag agatggtttg tgaatatgga atgagcgaga ttttaggtcc tctcaccctg
     3721 ggaaggaagc ataaagaagt ttttctgggt agagacattg ccgaggaccg caattacagt
     3781 gaagagattg cttatactat cgataaagaa gtgagagcaa taattgacca ggcttatgaa
     3841 aaagctcaag aaattatcaa tcgttaccgg gaaaaactgg atcttttagc tcaaagactg
     3901 gtggagaagg aagttctgga aagagaagag atagagcaaa ttctcgattt taatgaagaa
     3961 aatgagagct ctactctacc ccttcaaact cagattaacc ccgacctggc aggaggagaa
     4021 aaaaccgatg aaatttaata ctttgagaat tggtcttacg ggagtagctc aagctgtggt
     4081 aaacgatgaa aacgctgctg atcattttgc tcccgatatg gttcctgctt ttgctactcc
     4141 tatgttgctc tctttaatgg ataatgctgc tcatgaagca gtaaagaacc accttcccga
     4201 gggctatgtc agtgtgggaa cacgtttgag tatttctcat gttgccgcta ctcccaaagg
     4261 tatgcaggtc actgcccggg caactctcgt ggagatccat cgaaaccgcc tgatatttac
     4321 agcagaagct tttgacgagg tagaaaaagt aggagaagga agattagaaa ggtttgtaat
     4381 aaatcgagat tctttcttga agaaattaga atcgaagcta aattttatta aagggttggt
     4441 tacagaataa ctgaggtgga ttttatgcga cagtttacgg tgtttggtct gggtatcttt
     4501 ggaagcagta tcgctattgc tctttatgaa caaggtttta ctgttttagg agtggatatt
     4561 gaagaagagc cggttaaaga attagcaggc aaaattactg aagtagttca ggctgatact
     4621 actgatgaaa aagttttaga ggctctggga ataaagaatt tcgatgtagc catagtgagt
     4681 atcggtaacg acattcaatc cagtgtttta actactttgg cagttaaaga gttaggtata
     4741 cctttagtta tagcccgggc cattaacgaa atgcatggga aaattcttca taaaatcggt
     4801 gctgaccggg ttatatttcc cgaacaggat actgctatac gagtggcgaa aactctggcc
     4861 tttccttatg ctatcagaag tgaagagcta tttcccggac ataacatcat cgagtctcaa
     4921 gtcccccctc ctctttatgg acttacatta accaggtctc aattgagaac tcgctatggg
     4981 attactgtga tagctataaa gcgaggagaa aactatattg tgtcaccaaa cgctacagaa
     5041 actttatcca aaggagatat tatttatatt ctaggtagcg aacagcaact taagaaattt
     5101 tttagagaga tggcagaact tagaaacgga aaggtaaagg tggaagaatg aaagctcagc
     5161 cagaatgttt ttgctgcagt ataagacagg ctcaagaagc tgctgaaata gcaggaaaag
     5221 ataaagatac tatctggaaa gtctctcagg aagtttgtgc agtttatgcc caggctaata
     5281 aagaatggac tcctgcttat atgactactt tagctcacga tgtggctaaa aaaattgtgg
     5341 gaatagaaga tatattttat caattaaaaa gagaccataa ccaagcagca ctccgatttt
     5401 atcctcagtt aaaagaatgg gttagaaaag gagataatcg gttagaacga gcagtaaagg
     5461 tggctatagc tggaaatctc atcgacctgg gggtttatcg gaatgttaaa gtagaagaag
     5521 tcttagaaga gattgaccat gctcgatggg gaagatacga attttcctcc tgggcaaagg
     5581 atttagaaaa tactcatact ttaatctatg tgggagataa cgccggggaa gtagtttttg
     5641 atcgaattct tttggaggag atatctccag ggagaaagat tttttttgtg gtgaaaggcg
     5701 gccccatatc taacgatgct ctcctagagg atgcccaaga agcaggtatc ggtgaggtag
     5761 cagagatttt aaccaccggt caggcagaag taggaatagc tttacaaaga gcaccttctt
     5821 ctcttcagaa attgtggaaa gaagcagata tgattatttc caaggggcag ggtaattttg
     5881 agactttgag cggagaagaa gggaatattt atttcttgct taaagctaaa tgtattcctg
     5941 tagctcggga gttaggagta gagcaaggag atttaatttt acagaaaaga aaagcttctc
     6001 ctcctgaggt tgaagtgtat tctagcggag ggttggtagt aagaaaaaac gatagccaat
     6061 ggcaagtgct tctggtaaaa aaaaatgatt cggggttgtg gactcttccc aaaggtcata
     6121 aggaaaaagg agagagagaa gaagaaactg cctgtcgaga agtagaggaa gaaaccggtt
     6181 ataaagttaa attaggacct cgagtgggag aaatcagttt cagctatgtt agaaattatc
     6241 aaccctttcg agaaatagct tccttttttc tcatggaagt agcaggagaa ggagaaaaag
     6301 aggaagaggg ggaaatcgca gaggtgaagt ggttccccat aagtgaagcg ttacgagtct
     6361 tgaactatga taatgaaaga agtttggttg gtaaggctct ggaactttta gaaagggaat
     6421 aaatgtggaa atttttgtaa aagttttttg ctttgtagac tattaaactc ttgcaaaatg
     6481 cattaaaatg cggtataagg agggatataa ggtatcctcc agaaatagaa agagattttc
     6541 ttagaaaaac ggcatttgtt aaacgctgag gaggttaaaa tatggagctt ttaaaaatat
     6601 cttctaaaac cagaccggtg tctttagctg gagcgttaac tggagtgatt agagaacagg
     6661 gtagagcgga aatgcaagca gtgggagcag gagcagtaaa tcaggcagta aaggctattg
     6721 ccattgctcg gggttatttg gctcccagcg gtatcgatct ggtttgtatt cctgcttttg
     6781 tggatgtgaa aattgatgat acggaaaaga cagctattag gtttatagta ctccccgctt
     6841 aaggggaaag taatagacta aaaataagga ggtaaggaaa gtatgaacaa gcaggaatta
     6901 gttaacaccc tttctgaaga gttaaaaaaa gaaggggtaa atgttaccaa gaaagatacg
     6961 gcaaagatcg tagatacgtt tttaggagtg attcaggaag aaatcaaaag aggcggtaag
     7021 gtacagttgg tggggtttgg gacttttgga gtgaaaacga gagcggcaag gagaggaaga
     7081 aacccgcaaa cgggtaagga aattcgtatt cctgaagcta caattccctt tttccgtccg
     7141 ggaaaaggcc ttaaagaact ggttaaagaa tagtgttaaa tgccaaacaa aaaggaggga
     7201 caatttgtcc ctcctttttg ttttataggg agagaaatgt ttctaggtga ttatcatctt
     7261 cacactcctc gctgtggaga tgcgaagggt agctatgatg aatatattga ggaggctttg
     7321 aggaaaggac ttagtgagat tggtttttcc ggccattgcc ctcaatattt tctttccccg
     7381 gaagaaagga aaagggagtc agctattccg gaagaggaat tggctctcta catcgaggaa
     7441 gtggaatctc tgcaagaaaa ataccggtct tctattacca tccgcttagg gttagaagta
     7501 gatttcattc ctggaaaaga agaagagcta catcctctgg ttgatttcta ttcctgggat
     7561 taccttttgc tttctgtcca ctaccttgga gactgggctt tcgataatcc tcgctatcta
     7621 tctcgctata aggaaagaaa tataaatgaa atttatgaaa gttattatca aactctcatc
     7681 gccggtatga aaactggttt ctttagcgct gttgctcatt ttgatttacc taaaaaattt
     7741 ggttttcggc ctaccggtga gattagggca gaggtagaag ctctgcgagt ttgtcaggag
     7801 caaaatctgg tactagagct caacactgct ggtcgacgta aaccggtggg ggaattttat
     7861 cccgcacctt ctattctttc ccaggcacag aagctgggtc tacaagtgtg tcttggctct
     7921 gatgctcacc agccagaaga tgtaggacag gattttaaag aagctctcgc acttttgcgt
     7981 gattcaggat ttgatcatat agtaggctgg gagaaaaggt ctgcagtttt gcatcctatt
     8041 tcttaaggag taaaagcgct gagactgatc accactatgg tggctctggt tttaatctct
     8101 ccccttttat atatagcttt tttagaaaac tatccccatt ctttctggac taaagtatcc
     8161 cgcaaaacct gctaaatcgc tcaagacttg gaaactatag ctaaccgcaa aatccattat
     8221 aaatactata aaggggtatt ctcctttata gttctgtttt ttcttcctca acgttggagt
     8281 gcatttatcc agcgtaattg gttttagttt ttcttattta ttttgctttt gtctttagcg
     8341 ccgtagactg tcttttgcgg ttaaccacaa cagtgtccag aaaaaaaatt atgggtaatg
     8401 atgaggttag gagagagcaa agaaagacat tctgcggatg attgaagcag tgctggagaa
     8461 agagaaggag aagaaaaaga atgattaact ggcgctttcc ctctgaatac cgaagccgga
     8521 ctgtggagcg ctgcgcttct tctttaccta aaatacattc tgaagacgag gcgctatttc
     8581 tcgctgaaaa gttagggata gaagttgtat ttgttcactt agaggaggct aaaggagggc
     8641 tcctctggag aggaaaagaa gcttacatcg tcatcaactc caaatttcca cccacggagc
     8701 gggctaaaac tattcttcat gaaatagctc attttctcct ccaccacgct aactatctgc
     8761 gcccggtgtt tttagaccac aagagcttta tcgccggttt agaaaaagaa gccgactact
     8821 tcgcctttct cttgatgcgg gagtggttgg aggaggagag cttgtaaagg catttaacag
     8881 gaagcatcaa taagcttgaa gaattaggta atagttaggg ttatgctgga cactgaggag
     8941 gataagtcaa agaaagcttt aagatatact cggcggatgt taaaagtagc cggtttaata
     9001 tcatcggaaa aatactcaag gacggaagtt ctgagctctt tatctgagcg cagagcttga
     9061 ggaaagcgct taaagaaggc agagacatga aatttataat cgtagatggt gcgaggagct
     9121 ttaccttgag ctttcttttc aaacaggaac agctctaaga aattatggaa gccttccgag
     9181 cggtggagcc ttactacttc ccccataaaa aacacctctt tctgggagtc agtttatagt
     9241 catcggactc agaggttttt ttagaggaag tggggaagcc gcagaaatgg ctctacaaac
     9301 cttctggtgg cccaacgggg aattgaaccc cggtttgcgg attgagagcc cgccgaacta
     9361 accactattc tattgggcca cgcccttatt atatttcctg ggaaagatac tgtcaacgtt
     9421 tgctacatgc ttc
//
LOCUS       DAOSVE010000070         9321 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_3965_length_9321_cov_76.311785, whole genome shotgun sequence.
ACCESSION   DAOSVE010000070 DAOSVE010000000
VERSION     DAOSVE010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 9321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9321
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3965_length_9321_cov_76.311785"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PK016_06475"
     CDS             <1..307
                     /locus_tag="PK016_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02741.1"
                     /translation="IYLRHQGELQILGIAVNENHGTGNLIASPEEYAQTIRAGFVNRY
                     GLTFPVLIDNYGKEHKKYIGSGVPAFLFLNEKGEIQYVEKSAISESELESLLYRYLL"
     gene            798..1889
                     /locus_tag="PK016_06480"
     CDS             798..1889
                     /locus_tag="PK016_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02742.1"
                     /translation="MMKRIVLPFTVCFLLFVLLTSVGAQEEVASPLVRDLESMEKILY
                     GLPQSGSVLSRVEKIERDLIGDTLSGTLMERVNTLKTFILTGTDAEPSLDFKIRAIRL
                     ALRSEPAKSGILIAELEELEKLIFGVVSDEPIGVRVDRLYKTCIDPSKVKSFTVQIPE
                     ETLVKISIQRELHSERNNEGDPVPFIVAEDISIDGVLVLAKGTEGEGRLTKVRRKGAF
                     GKSGRLEIDFGAVRASDGTLIPLGLGDKSIEENKAVGYAVITSIAGLALLGPIGAVAG
                     VFIQGEPAKVEKGAELIVEVLETKEVAGPLVGVGEMVLPSPPEISEYESSWEEEIPQE
                     NIEELPQEEEPITQEEPEVEIEIKPYEEW"
     gene            1906..2676
                     /locus_tag="PK016_06485"
     CDS             1906..2676
                     /locus_tag="PK016_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02743.1"
                     /translation="MKNKIVMTMTLIFVLVLMVGMAEGQVDYDKIYQNTAKKWTRSAQ
                     VEDAFSVEALFWNEEVVQAWVAKYGKENLLSAEEELAYHRDFLQRERMNRYLVFEVTI
                     KKLKGAPLYPMKFAQNTYLVDDRGNKFYPLEFPPEFEEKIFDKVTGKIYFPRFDKEGN
                     PIINPDTRSITLHLARISIDPKLIGKEINLTWDNPYLPPDYSRPEWKPVLEEEILRLE
                     ERVKELELQKRMLEDKIKGIDNEIEEVKAKIKDLQKEQ"
     gene            2673..4256
                     /locus_tag="PK016_06490"
     CDS             2673..4256
                     /locus_tag="PK016_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02744.1"
                     /translation="MKKIKFEKLFLFFFLFLFCPSSLLAQEGIKIKADLVLYDQEREE
                     VSASGNVKVEWHDIQVTTREITFFISTQELWVPYSLEATFGDNQVQGEQFYYSLLRDE
                     GWVKKAELIYKIEEEGKLYFRGDKIEYLKGEWTGNNLLLTGCEKEPPLYSLRAKEVVI
                     YPQDRLVLEGLSLYLREKKIVELPLYSRVLGEGESTFSPALGYSRKEGWYIGSYYDYL
                     VTKDLLLKSRLNISSVQGVELSLDLIASLPQGEGRIFWDIYPGAIDTQGGYVHFEKNN
                     LSLWALSVSNERVGENFVSRSPQLIVSYGETPDEGFNWQSTFSWGYFEEDEFSTWRKD
                     LYLNAGWKGKEGGLEFFFWNIDLAGGQIIPGWGGKIWWEKEISSEWNLNLSYQFSEVQ
                     GESPFSFDPQDQRIFSLDSCWGDYRESFLRLRGDYDLDEGSWKELTAGLGLGDESLSL
                     GVEGVYSFGLEEWKEERYFVRKKIEDCITLEASWYEPEGSLFLSLNLSGLDTGKKTES
                     LFDEKEEFSPFELEREGGN"
     gene            4258..4686
                     /locus_tag="PK016_06495"
     CDS             4258..4686
                     /locus_tag="PK016_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HXL02745.1"
                     /translation="MEKRLYRSRTDKMLGGVCGGIAKYFNVDPTLIRLLAAALILAGG
                     AIIIAYIIAWIVIPEEPEGKTEEEEKVIVATTPEEKSEERRKDIELIAWILIFVGLIW
                     FCLHLFSLWLPSRFFARLIFPVALVIVGVLLLWERKKEKR"
     gene            5447..5848
                     /locus_tag="PK016_06500"
     CDS             5447..5848
                     /locus_tag="PK016_06500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HXL02746.1"
                     /translation="MHLVQISEAASLAIHSMALLIQNRGSLLTVKDMAQECQASEAHL
                     AKVMQHLAKAGLVTANRGPQGGYTLTKEGEEANILTIYEAVEGPIVPRECLLKNKICL
                     FPACIFGGVIEKAEREIKDYFASKKLKDLVV"
     gene            5870..7513
                     /gene="hcp"
                     /locus_tag="PK016_06505"
                     /gene_synonym="priS"
     CDS             5870..7513
                     /gene="hcp"
                     /locus_tag="PK016_06505"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HXL02747.1"
                     /translation="MFCYQCEQTAGGTGCTNFGVCGKDPETAALQDLLIYAVQGISRY
                     AKRAQDLGVRDREIDVFILEALFTTVTNVDFDPRRVEELIRRAYQMKEKAKNIYLSVC
                     AQKGISPEELSGPAAWVPASNFDELVKQGEEVTLEKDIERWGESVAGAKHLIIYGLKG
                     MAAYADHAQILGVEDEEIYAFFHRTLDYLASDNFTLEELLSRALQVGEYNLKVMALLD
                     QAHTSTYGHPVPTPVRITPKAGKAILISGHDLKDLEELLKQTEGRGINVYTHGEMLPA
                     HGYPSLKKYPHLVGNYGSAWQNQQKEFDVFPGAILMTTNCIQRPKESYKNRIFTSGLV
                     AWPGVTHISNRDFTPVIEAALKERGFPEDEEEKDITVGFARNAVLGVADKVIEGVKKG
                     KIRHFFLVGGCDGAKPGRNYYTQFAQMVPSDCLILTLACGKYRFNKLEFGDIDGIPRL
                     LDVGQCNDAYSAIQIALALADAFQTDVNSLPLSLVLSWYEQKAIAILLTLLHLGIKNM
                     RLGPTMPAFVKDPVYKVLKEQFNLIPITTPEEDLKAILG"
     gene            complement(7573..8079)
                     /locus_tag="PK016_06510"
     CDS             complement(7573..8079)
                     /locus_tag="PK016_06510"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HXL02748.1"
                     /translation="MKKSLKPQNWFLLLFLGLSSILLLYGCVPTPTFEKAPDFTLLDT
                     EGNSFTLSEKEGQPIVLCFFTTGCPRCQMEVPYLNSVYEEYKDTSELLVVGIAVGESQ
                     NEVQNFIDTYSVEYPVLLDSQEEVADMYGILNVPHLFFINREGYIEEDHTELMDQSAL
                     ENCVQEII"
     gene            complement(8076..8609)
                     /locus_tag="PK016_06515"
     CDS             complement(8076..8609)
                     /locus_tag="PK016_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HXL02749.1"
                     /translation="MRKWWFILILGLCLLSFFPGKGYTQEELLSVRVIKCTRPHYLKA
                     EVISPDEEKEVSIKLAGIRSPIERAEIYKKALKRVRELTLNQDLKFDFALGHSEEEEV
                     WVGYLYIPEGEEWKIVNAELLREGLVELNEKTAGENMVGYLISAQEEAQEQKVGLWSV
                     PEPQKRENREECPSCTR"
BASE COUNT         2853 a         1557 c         2086 g         2825 t
ORIGIN      
        1 aatttatctc cggcatcaag gagaattaca aattttggga atagcggtga atgaaaacca
       61 tggcactgga aatttaattg cttctccaga agaatatgct caaacgatca gagctggttt
      121 tgttaatagg tacggcttga cttttccggt tttaattgat aattatggaa aagagcataa
      181 aaaatatata gggtctggtg tcccggcctt tcttttctta aacgaaaaag gggaaatcca
      241 gtatgttgag aaaagtgcta tttcagaaag cgaactggaa tctcttttat atcgatatct
      301 tttgtaagat gcatgatgga gtgatgcggc ggtattagag accgccgcat cactccaggt
      361 gtttccctcg acgttatgtt ttagtttttt tcatctgttt tgcttttaat tttaggctat
      421 caagctgctt aatatactcc tttgtgataa tagaatgaaa agaaaggcaa cttagcagat
      481 ttttttaaat tttaggaagt tcttttttcc agtggtgtat cttcttccta ggttctggtg
      541 ggattggtaa ctggttttgc taatagtcat ttttcttgta taaataaggt aataaacaat
      601 atagaattct tttattttgc ttcattagta aatatccctc ttttaatttt agctaatttg
      661 agggaatatc ttcttgagaa actccagggt gacattgtcc cataataact ataaaagtgg
      721 atggaggttg gagaaaaaaa gccttttttg gagtataata aaattgagga taaatgagct
      781 ttagtggaga gtgaaggatg atgaaaagaa tagttttacc tttcactgtt tgttttttac
      841 tttttgtttt actgacttcg gtaggggctc aagaagaggt agcttccccg ttggtgagag
      901 atttagagag catggaaaaa attctttatg gtttaccgca gtctggaagc gtgctgagca
      961 gagtagaaaa aatagaaaga gatttaattg gtgacactct ctctggaact cttatggaga
     1021 gagtcaacac cctcaagact ttcatcctta ccggtacaga tgctgagcca tctttagact
     1081 ttaagattag agctattcgt ctagctctcc gttctgaacc ggcaaagagt ggaattctta
     1141 ttgctgaatt agaggaactg gaaaaattga tttttggagt ggtcagtgat gaacccatag
     1201 gagtaagagt agaccgcctg tacaaaactt gtatagatcc ttctaaagta aaatctttta
     1261 ctgttcagat tccggaagaa actctggtta aaatctctat tcagagggag ctccactcag
     1321 agagaaacaa tgaaggtgat ccagttcctt tcatagtggc agaggatata agcatagatg
     1381 gggtcttagt cttggctaaa ggaacagaag gggaggggag attaactaaa gtcagacgta
     1441 agggagcgtt tggcaaaagt ggacgtttgg aaatagattt tggggctgta agagccagtg
     1501 atggaactct tattcctttg ggtttgggag acaaatcaat tgaagaaaat aaagcagtag
     1561 gatatgcagt aataaccagt attgctggac ttgccttgct tggtcctata ggagcagtag
     1621 ctggagtttt tatacaagga gaaccagcta aagtggaaaa aggggcagag ctaattgtag
     1681 aggtactgga gaccaaagaa gttgctgggc ctctggtagg ggtaggagaa atggtattgc
     1741 catccccacc agaaatctca gaatatgaga gctcctggga agaagaaatt ccccaagaaa
     1801 atattgaaga acttcctcag gaggaagaac ctataactca agaggagcct gaagtagaga
     1861 tagagataaa accttatgaa gaatggtgat aaggaggttt aaggaatgaa aaacaaaata
     1921 gttatgacta tgactctgat ttttgttctg gtactgatgg tgggtatggc agaggggcag
     1981 gttgattatg acaagatata ccagaataca gccaagaaat ggactcgctc tgctcaggta
     2041 gaggatgctt tttctgtgga ggctttattc tggaatgaag aggtggttca ggcctgggta
     2101 gctaagtacg gcaaggaaaa tttgctttct gcagaagaag aactggctta ccatcgagat
     2161 tttttgcaaa gagagagaat gaatcgttat ttagtattcg aggtaactat taaaaaactt
     2221 aaaggagcgc ctctttatcc tatgaaattt gctcaaaata cttatttagt cgatgaccgg
     2281 ggaaataaat tttatccttt agagtttcct cccgaatttg aagaaaaaat cttcgataaa
     2341 gtaacaggga aaatttattt tcctcgattt gacaaagagg ggaatcctat aattaacccg
     2401 gatactcgct ctattactct tcatcttgcc cggatttcca ttgaccctaa attaattggt
     2461 aaggagatca atcttacctg ggataatcct tatcttcctc ctgattattc tcgtccggag
     2521 tggaaaccag tcctggaaga agagatttta cgtttagaag aacgagtgaa agaactggaa
     2581 ctacagaaga gaatgctgga agacaaaata aaagggatag ataatgaaat agaagaggta
     2641 aaagctaaga ttaaagactt acaaaaggaa cagtgaaaaa aataaagttt gagaaattgt
     2701 ttttattttt tttcctgttt ttgttttgcc cttcttctct tttggcacaa gaaggaataa
     2761 aaattaaagc agacctcgta ctttatgacc aggaaagaga agaagttagc gcttcaggta
     2821 atgtaaaggt agaatggcac gatattcagg tcactacccg ggaaattacc ttttttattt
     2881 ctactcagga attgtgggtt ccttattcat tagaagctac tttcggtgat aaccaggtac
     2941 agggagagca attttattat tctctcttac gtgatgaggg atgggtaaag aaggcagaat
     3001 taatttataa aattgaggaa gaggggaagc tctattttcg gggagataaa atagaatatt
     3061 taaaaggtga gtggacaggt aataaccttt tacttactgg atgcgaaaag gagcctcctc
     3121 tttatagctt aagagctaaa gaggtggtta tttatcctca agatagattg gttctagaag
     3181 gattgagttt ataccttaga gaaaagaaaa tagtggaatt gccgctttat tctcgggtgt
     3241 taggagaagg agaaagtact ttttctcccg ctttagggta tagtcgtaag gaaggatggt
     3301 atataggtag ttactatgat taccttgtta ctaaagatct acttttaaag tcccggttga
     3361 atatctcttc tgtgcaagga gttgagctaa gtctggattt aattgcttct cttcctcagg
     3421 gagagggaag aattttttgg gatatctatc caggcgcaat agatacccag ggaggatacg
     3481 tacattttga gaaaaacaat ctttctctct gggctttaag cgtaagtaat gagagagtgg
     3541 gcgaaaattt tgtttctcgc tctcctcaac tgatagtttc ttatggagaa actccggatg
     3601 aaggttttaa ctggcaaagc acttttagct ggggatattt cgaagaggat gagttttcca
     3661 cttggagaaa agacctttat ttgaatgcag ggtggaaggg aaaggaggga ggactggaat
     3721 tttttttctg gaatatagac ctggctggag ggcaaataat acccggttgg ggaggtaaaa
     3781 tatggtggga gaaggagatt tcctctgagt ggaatcttaa tctctcttat cagtttagcg
     3841 aagtgcaagg agaaagccct ttttcctttg acccacagga tcaaaggatt ttttctctgg
     3901 actcctgctg gggagactat agggaaagtt ttctaagatt gcggggtgac tatgaccttg
     3961 atgaaggaag ctggaaagag ttaaccgccg ggttaggatt aggagatgaa agcctttctt
     4021 tgggagtgga gggagtttat tctttcgggt tggaagagtg gaaagaggaa aggtattttg
     4081 tgcgcaagaa gatagaagac tgtataactt tggaggcttc ctggtatgaa ccggaaggta
     4141 gtttattttt atctttgaat ctatctggtt tagatacagg taaaaaaact gagagtcttt
     4201 ttgatgaaaa agaagagttt agtccttttg aattagagag agagggagga aattaatttg
     4261 gaaaagagat tgtatcgttc tcgaacagac aagatgttag gaggagtatg tggaggaatt
     4321 gctaagtatt tcaatgttga tcccacttta ataagacttt tagctgcggc tctaatttta
     4381 gctggaggag caattatcat tgcttacatc atagcctgga ttgttatccc tgaagagcct
     4441 gaaggaaaga cagaagaaga ggaaaaagta atagtagcaa caacccctga agaaaagagt
     4501 gaagagagaa gaaaggatat agaactgatt gcctggatct taatttttgt gggactgata
     4561 tggttttgcc ttcatctttt ctctttgtgg cttccttccc gtttttttgc ccgtttaatt
     4621 ttcccggttg ccctggttat agttggcgtt ttgttattgt gggaaagaaa gaaggagaag
     4681 cgataggaat cacggagaaa aaagctaaag tacatctccc gaccttgaca cctcagttaa
     4741 ctgaaaccct cacccaatct atattcttta acttatcatc tgatattttg tcactccact
     4801 gattatttgc agtagtttat aaaaatactt tttcccggga gtggtatttc caggtggttt
     4861 aaaccttggc agttttttag aagccagtta tcaacttagt tactttagaa gaagctaaaa
     4921 atttagccac tcgaggatgt atcgagtagc ttgtatggga ataaggttat cattgaaggg
     4981 ataaccttta cgataaaatt ttgagaatta tagtatcctg caaaactggt taaaatatta
     5041 aaactttaaa aagtaaatta tatcgaaagt caaaaactca aggtcgctac gcatagagaa
     5101 agactttgtc tttctctatg gcctttattt cttttgtttt ttaagttttc ttaatcttgc
     5161 gtcgcagact gcctttttct ccagcatact ggtggatatt ttcccaccag tagtttttgt
     5221 ttatttgctt tttgggggat tataaagggg tgctcccctt tatagtaatt ataattagtt
     5281 ttgcggttaa ctataatctg ggaaacattt atcgagtttt cttcttatga ccacatcggg
     5341 aagaagaact tcttgcgatg aagtatcagg taaaatatct ttttgagatt gtctcttgac
     5401 aaatttaggt atttaggtat cataatacct gaaaggagaa aaaaagatgc atttagttca
     5461 aatttcagaa gcagcctctt tagctatcca cagtatggct cttttaattc aaaatagagg
     5521 aagcctcctc accgtcaaag atatggccca agaatgccag gcttcagaag ctcatttagc
     5581 taaagtgatg caacatttag ctaaggcggg gttagtaact gctaaccgag gccctcaagg
     5641 aggttacact ttaaccaaag agggggaaga agccaatatt ttgactattt acgaagcagt
     5701 ggaggggcct attgtccctc gagagtgttt actcaaaaac aaaatttgtc tttttccggc
     5761 ctgtattttt ggaggggtaa tagaaaaggc agaaagagaa ataaaagact attttgccag
     5821 taaaaaattg aaagatttag ttgtataaaa gcataaggag gtgttttgca tgttttgtta
     5881 tcagtgtgag caaactgcag gaggaacagg ttgtactaat tttggagtat gcggtaaaga
     5941 cccggaaact gcagccttac aagacctttt aatatatgct gtccaaggaa tttctaggta
     6001 tgctaaaaga gcgcaagact taggggttcg agaccgagaa attgatgttt ttatcctgga
     6061 agctcttttt accactgtta ccaatgttga ttttgacccc cgaagagtag aggaactaat
     6121 tcgtcgagct tatcagatga aagagaaagc taaaaatata tatctttctg tctgtgctca
     6181 aaaaggaatt tcaccagaag agctttctgg ccctgctgcc tgggtcccgg cttctaattt
     6241 tgacgaatta gtaaaacaag gggaagaagt aactctggaa aaagatatag aacgatgggg
     6301 agaatcggta gctggagcca aacatttaat tatttatggg ctcaaaggaa tggcagctta
     6361 tgctgatcat gcccaaattt taggagtaga agatgaggaa atatatgcct ttttccaccg
     6421 cactcttgat tatttagcca gcgacaattt tactctggag gaacttctct cccgtgcctt
     6481 acaggtggga gaatacaatc taaaagtaat ggctcttctg gatcaagctc acacttctac
     6541 ttatggtcat cctgtcccta cgccggtgag aataactccc aaggcaggta aagctattct
     6601 aatatctggt catgacttga aggatttaga agaactcctc aaacagactg agggtagagg
     6661 tatcaatgtt tatactcatg gggaaatgct tcctgctcac ggttatccga gtctcaaaaa
     6721 atatcctcat ctggtgggaa attatggcag tgcctggcaa aatcaacaaa aggaatttga
     6781 tgtctttcct ggagctatac tgatgactac caattgcatt caaagaccta aagaaagcta
     6841 taaaaatagg atttttacta gtggcctagt agcctggcca ggagtaaccc atataagtaa
     6901 tcgagacttc actccggtaa ttgaagctgc tctgaaagaa agaggtttcc ctgaggatga
     6961 agaagagaaa gacataacgg tagggttcgc tcgcaacgca gttctggggg tagcggataa
     7021 ggtaattgaa ggagtgaaaa aaggcaaaat ccgccatttc tttttggtcg gaggatgtga
     7081 cggagcaaaa ccgggaagaa attattacac tcaatttgct caaatggttc cttccgattg
     7141 tctcatactt actttagcct gtggtaaata tcgcttcaat aagttagagt ttggagatat
     7201 tgatggtatc cctcggctcc ttgatgtagg gcagtgtaat gatgcttatt ctgctattca
     7261 gattgctttg gctttagctg atgctttcca aactgatgtt aattctcttc ctctttcgct
     7321 agttctttcc tggtatgaac aaaaggcgat agctatttta ctcactcttc ttcatctggg
     7381 aataaaaaat atgcgcttag gacccactat gcctgctttc gtaaaagacc cggtttataa
     7441 agtgctcaaa gaacaattta atcttattcc tattaccaca ccagaggagg atttgaaagc
     7501 tatattagga taaatcgccg gggaggaaga agcctccccg gtaacaatta tggtagggct
     7561 ggtagtttta atctaaatta tctcttgtac acagttctct aaggcacttt gatccattag
     7621 ctcggtatgg tcttcttcaa tatatccttc ccggttaata aagaaaaggt gaggaacgtt
     7681 aagaattcca tacatatcgg ctacttcttc ttgggagtca agaagaacgg ggtattctac
     7741 gctataggta tctatgaaat tttgtacctc attttgagat tcacctacgg cgattcctac
     7801 tactaagagt tcggaagtat ctttatattc ttcgtagacg gaattgagat aaggtacttc
     7861 catttggcag cgaggacaac cagtggtgaa aaagcacagt actatcggtt gtccttcttt
     7921 ttcacttagg gtaaaggaat ttccttcggt atctaggaga gtaaaatctg gtgctttctc
     7981 gaaagtgggt gttggtacac agccataaag taatagaatg ctacttaatc ctaaaaataa
     8041 caaaagaaac caattttgag gctttaagct ttttttcatc ttgtgcaact gggacactcc
     8101 tctctgtttt ctcttttctg gggttcaggc acagaccaca gccctacttt ttgctcctgt
     8161 gcttcttctt gagcagaaat tagataaccc accatgtttt ctcctgctgt tttttcgttt
     8221 aattctacca atccttccct tagaagttca gcgttaacta ttttccactc ctctccttcc
     8281 gggatataga ggtatcccac ccatacttcc tcttcttcac tgtgacctaa agcgaaatca
     8341 aattttagat cctggtttaa agttagctct cttacccttt ttaaagcttt tttgtagatt
     8401 tcggctctct ctataggaga cctgattcct gctaacttta tggaaacttc tttttcctca
     8461 tcaggagaaa taacttctgc ttttagataa tgaggacgtg tacactttat cactctaact
     8521 gataaaagtt cttcctgagt atatcctttt ccagggaaga aggagagaag acataatcct
     8581 aatattaaga taaaccacca tttccgcatt ttttcacctc ctttcggaat atacgacttc
     8641 taaaatcgga tagtttcaat tatagttaac cattaaaaca atgtaaaatt cttttcgcgg
     8701 caactccgaa ccatggaagg gaagcggatt tatccccctt ataacctcat cgttacccat
     8761 aatttttctc tggacactat tatggttaac ccaaaagaca gcctgcgacg caagaaaaaa
     8821 agcaaattag attaaaaaga attcggaaca attacgctgg agaaaagcac tccagcgttg
     8881 aggaagggaa aacagaacta taggagaaac cgatccccct attaccccct tggaaaaaca
     8941 aaaaacataa agaataaaaa ccgctgagga agggaaaata tcttcctcag cggatgcctg
     9001 ataaaagcgc tcaggaatga tgaaataaag ggaatgcctc tcatgtctgt cattcctgaa
     9061 tgtatttatc aggaatccaa attatttagg ggacaagact atttagattc ccgattaaag
     9121 atttcgggaa tgacgaatga tgtggatgcc tgataaaagc gctcaggcat gacagagggg
     9181 aagccactcc ttgtgtccgt cattcctgaa tgtatttatc aggaatctaa actatagggg
     9241 gacaagatta tttagattcc cgattgaaga tttcgggaat gacaaaaaag ggtgtcattc
     9301 ctgaatgtct ctatcaggaa t
//
LOCUS       DAOSVE010000071         8997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_4119_length_8997_cov_64.554350, whole genome shotgun sequence.
ACCESSION   DAOSVE010000071 DAOSVE010000000
VERSION     DAOSVE010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 8997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8997
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4119_length_8997_cov_64.554350"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3058)
                     /locus_tag="PK016_06520"
     CDS             complement(<1..3058)
                     /locus_tag="PK016_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02750.1"
                     /translation="MLYPRKLNELSKELFKNPTSEYRGAPFWAWNTKLIKDELLEQID
                     NFKIMGMGGFHIHSRTGLAIDYLGEEFMGLVKSCNEKAKEEGMLCWLYDEDRWPSGFA
                     GGYVTKDSRYRERFLVFTPFLYENMEEEISHLLPGGRTVKGKNRKLLARYEVTLEDGY
                     LVYYQRLKEEETGKQGKIWYAYLEISGDDPWFNNQAYVDTLNREAIEKFIEVTHQRYY
                     EVLGEDFGKSIPAIFTDEPQFMRKDCLSFSEEEREVILPFTDDLEESYYEIYNQSLLD
                     HLPELIWELPDNKTSLVRYRYHDHVTERFVQAFADTIGEWCEKHGIMLTGHMMEESTL
                     ESQTAAIGEAMRPLRSFGLPGIDMLCDWREFSTAKQAQSIAHQYGREGVLSEIYGVTN
                     WDFDFRGYKLAGDWQAALGVTVRVHHLTWVSMEGEAKRDYPPSIGYQVPWYREYSLIE
                     DHFSRLNTVLSRGKADVKVGVIHPVESYWIHWGPKDKTLLKRAELETNFKNIIEWLLF
                     GLIDFDFVSESLLPSLSKVQESNGFEVGEMSYEVIIVPHCEILRSTTLERLEAFQKAG
                     GEIIFLGEPPTLVDGVFSDRAKKLADKSSVIPFSRTKLGEKLDKYRDIEIWKEDGTPS
                     DNLFYQMRKEGEKKWLFISHVHKMRNPDIARLEKIKIKIRGEWNPEIYDTLTGEVKEC
                     AAEIKDGNTLIEYEFYPHDSLLLELTPGMPGEKEGQKNSYSTTREVNLLDPVSFSLSE
                     PNVLLLDMAEYAFDNGGWQPQEELLRIDNKFREILNYPLRMNKIAQPWTDKKVEPYEH
                     VLRLKFVVTSHIEIKDLKLALENPENTKIFVNGEHIESEVTGWFVDKSIKKVPIPGLP
                     IGDSEIVLEIPFNSKTNVEWCYLLGNFGVEVHGRHTKIVESVEKLAFGSWTEERLPFY
                     AGNVTYHCLVECDEGELIIEVPQFRAPLLSISLDGEEKGKIAFAPYVLSLGWVEKGEH
                     SIDITAYGNRVNAFGAVHNCDDTYFWFGPNAWRTTGNQWSYEYRLKP"
     gene            complement(3087..5771)
                     /locus_tag="PK016_06525"
     CDS             complement(3087..5771)
                     /locus_tag="PK016_06525"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HXL02751.1"
                     /translation="MTISIRDMYCNYSTTPLALKNGKPKFLWIIDSDKKRGKQTAFQL
                     LVSSFPDLMKENKGDMWDTQKVESSENYCYYEGKELESFKRYYWKVRVWDEEGGMSDW
                     SSTSCFETGPLEKKDWKAQWIAKKDLKTFKSSGDYDTESYKQYYASFFRKPFELRNGI
                     KKARAYISGLGFYELYLNGKKIGNNVLDPGQTEYSKEALYSVYDITNYLSLKNCIGVI
                     LGNGRYIERYGYSKPRLVLQILIEYEDGNKEFIFSDNSWKSSHGPIQENGIYYGEKYD
                     ARLEMPNWNIYHFDDTNWEETEIVSGPDLRYQSMPPIRVTSALKPQKMYSIKPGVYIY
                     DFGQNFTGWVKIAVKGPRGTEIKIRHSELINKDGTLNTATIRKAEATDTYVLKGEGLE
                     TYEPRFTYHGFRYIEVSGFPTVPTIESVEGCFVHTDVEKIGDFYCSNELINKVHNNIL
                     WGQLSNLHSIPTDCPQRDERFGWMGDAQLSAEEAIYNFDMNLFYENYLQEIKLSQKED
                     GSIPDVVPAYIKIYPADPAWGSAYITLAWYLYEYYKNEQVLRNHFDSMKKYVEFLKDN
                     SENNLIKKLGKFGDWCPPGAISPKKTPVEVTSSWYYYNDVYLFSRICEVLGEEDEAKY
                     YLELSEKIKNSFNGGFLKEYGYETRMFGPVDRLISQTSQILPLYYEMVPKDKKEFILE
                     RLIDNIVEHQDSHVDTGIVGTRYLFDVLSENGYADLAFKIITQESYPGWGYMIKEGAT
                     TVWERWEKLEAGGMNSQNHIMLGSVDTWFYKYLVGLSLTKPGWEEVKIKPHLVSDVSF
                     ASGKVKTVKGEFLVSWEKAETTFKLSIAIPFGCEANIFIPRLGEEYVLNESGVLLVDS
                     SKKQDNLPESKIKFEGIEEDSIIVNVGSGQYYFELKSK"
     gene            complement(5957..8374)
                     /locus_tag="PK016_06530"
     CDS             complement(5957..8374)
                     /locus_tag="PK016_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006860132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HXL02752.1"
                     /translation="MEIYKDKNRSISERVEDLLSRMTLQQKVAQLQCTMLMSDPDQGL
                     QGFPEGIGEVTGTTSIGKTIEANAELAKRVQDIIRAKNDLGIPVLLHSEALTGLMAAG
                     ATVFPSAIGLGATWNPDTVEKMTNIIRKQMLAVGVRQALSPVMDVARDPRWGRIGETY
                     GEDPTLCSAMSVAFTKGLQGDELKNGIVATGKHFLGYGFCEGGLNMSSNPIPPRELRE
                     VYAKPFQAAITEGGLKSIMNSYGTIDGEMVIKSKHILTDLLRQEMGFDGLVVSDYMSI
                     KRACDLKASPDPKWAGIEALQAGLDIELPFPYGYTQTLIEAVNEGIIDESLIDCSVRR
                     ILKTKFELGLFEDPYPRVDLIAESYENKAATAHSLKAARESIVLLKNDGILPLNKDIK
                     NIAVIGPHGNSLRLLFGCYTYPAMLEMMLTETMSEMAGTLDMAGSDLETNKVGDTQSL
                     PPFFEGSNVRQEPPIVEETIKKLYGAITPTILDSIKAKCPDANVEYERGCDVAGTNRG
                     GFAAAIAAAKRADVVILVLGGKYGWGNTCTIGEGIDSDRIGLPGVQEELAKAIFETGT
                     PAIFVHMDARPLSSEYIAKNYPAIIENWFPGITGGSALADVLFGDYNPAGRLPVTVAR
                     NAGQIPIYARHRKGDSYDEVKGMVMNMYAEGSKQPLFYFGEGKSYTQFEYSNLKVDKK
                     VKADGVINLSCEVTNIGGRDGEEVVQIYVRDEVASMLRPSKELAGFKRVALKVGETKT
                     VHFSMRADQFAFLDVDMNWIVEAGTMTVMVGGSSEDIRLSDTFEIENTAYVVGKDRGF
                     FARAKVE"
     gene            complement(8748..>8997)
                     /locus_tag="PK016_06535"
     CDS             complement(8748..>8997)
                     /locus_tag="PK016_06535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL02753.1"
                     /translation="IKPLHPYSKLLKESLLQPIPKKRRESRVKKASFEVEEFNLPGCK
                     FAERCSCAEGICGRENPEEYYVEGRVVRCFLYERERSN"
BASE COUNT         2531 a         2103 c         1390 g         2973 t
ORIGIN      
        1 ctggtttgag cctgtattca taagaccatt gattgccagt agttctccag gcattcgggc
       61 caaaccaaaa ataggtatcg tcacagttgt gcacggcacc aaaagcattt actctatttc
      121 cgtaggcggt aatgtcaatt gagtgttccc ctttctctac ccagcccagg ctcagcacat
      181 aaggagcaaa agctatttta cctttttctt ctccatccag agatattgat agtaaaggag
      241 ctctgaattg aggtacttca attattaatt ctccttcatc acactccacc agacaatgat
      301 aagtgacatt tccagcataa aagggcagtc tctcttcagt ccagcttcca aaagcaagct
      361 tctctaccga ttctacaatt ttagtgtgtc ttccgtggac ctccactcca aaatttccca
      421 atagatagca ccattccacg ttggtttttg aattaaacgg tatttccaat acaatttccg
      481 aatctccgat ggggagacct ggaatcggga ccttttttat gcttttatcc acgaaccagc
      541 cagtaacttc agattcgata tgttctccat taacaaatat tttagtattt tccggattct
      601 cgagagccaa ctttaggtcc tttatttcta tgtgtgaggt aactacaaat tttaacctca
      661 gcacatgctc atatggctct acttttttat cagtccatgg ctgagctatt ttgttcattc
      721 gtagagggta attcagaatt tctctaaatt tgttatctat cctgagcagc tcttcttgtg
      781 gttgccaacc tccattatca aatgcgtact ctgccatatc cagcaaaagc acattaggct
      841 ccgataaaga aaaagaaacg ggatcgagca aatttacctc ccttgtagtt gaatagctgt
      901 ttttttgccc ttctttttcc ccgggcatcc ccggtgtaag ttccagcagt aaactatcat
      961 gaggataaaa ttcgtattct attaatgtgt ttccatcttt aatttctgca gcgcactcct
     1021 ttacctcccc cgttaaagtg tcatagattt caggattcca ttcccctctt atctttattt
     1081 ttattttttc taacctcgca atatcggggt ttctcatttt atggacgtga gaaataaata
     1141 accatttctt ttctccttct tttctcattt gataaaataa attatcggaa ggagtgccat
     1201 cttctttcca tatttctata tcacgatatt tatcaagctt ctcccctaat ttagtcctgg
     1261 aaaaaggaat aacggaggac ttatctgcta atttttttgc cctatctgag aaaacaccat
     1321 ctaccagggt ggggggctcg cctaaaaata taatctcccc accagctttc tggaaagcct
     1381 ctaatctctc taaagtagtg gagcgaagaa tttcgcaatg ggggacaatt attacctcat
     1441 aagacatttc ccccacttca aaaccattac tttcctggac tttcgataac gaaggcagca
     1501 atgattcgga tacaaaatca aaatcaataa gcccgaacaa aagccactct atgatgtttt
     1561 taaagttggt ttctaattcc gctcttttaa gtagagtttt atcttttggc ccccagtgaa
     1621 tccagtaaga ttcaacagga tgaataactc ctaccttaac atcggccttt ccccggctga
     1681 gaaccgtatt caagcggcta aaatggtctt caatcagaga atactctcta taccagggaa
     1741 cttgataccc aatactggga ggataatctc tctttgcttc tccttccatt gatacccagg
     1801 tgagatgatg aactctaaca gtaactccta atgcagcttg ccagtcacct gctaacttgt
     1861 aacctctaaa atcaaaatcc cagttggtaa ctccatatat ctcacttaaa actccttctc
     1921 taccgtattg atgagctatg ctttgagctt gtttggcagt ggagaattct ctccaatcac
     1981 acaacatatc aattccaggc agcccgaaag acctgagtgg cctcatagct tctcctatag
     2041 ctgcagtttg cgattccaaa gtcgattctt ccatcatatg acccgttaac ataatcccat
     2101 gtttttcaca ccattctcca atagtgtcag caaaagcctg aacaaacctt tctgttacgt
     2161 gatcgtgata acggtatcta acgagagaag ttttattatc tggtaactcc cagatcagct
     2221 ctggcaggtg gtctaacaag ctttgattat atatctcata ataactttct tctaaatcat
     2281 ctgtgaaagg aagaatcact tctctttctt cttcagaaaa gctcaaacag tcttttctca
     2341 taaattgggg ctcatcagtg aagatagccg ggatagattt gccaaagtct tcacccaaca
     2401 cttcgtaata tctctggtga gttacctcga tgaatttttc aattgcttcc cgattgagtg
     2461 tatcaacata ggcttgatta ttgaaccagg ggtcatctcc cgatatttct aaataggcat
     2521 accagatttt tccctgtttt cccgtttcct cttcttttaa cctctggtaa taaactaaat
     2581 aaccatcttc taaagtcacc tcataccggg ccagcaattt cctattttta cctttaactg
     2641 ttcttcctcc cggaagtaaa tgtgatattt cttcctccat attttcatat agaaaaggag
     2701 tgaagaccaa aaaacgttct ctgtatcggc tatctttcgt aacgtatcca ccggcaaagc
     2761 ccgatggcca cctatcttcg tcgtatagcc agcatagcat cccttcttct ttggcctttt
     2821 cattgcagga ttttactaaa cccataaatt cttcacctaa ataatcaata gctaacccgg
     2881 tgcgggaatg gatatgaaag cctcccattc ccataatttt aaaattgtca atttgttcca
     2941 gcagttcatc tttgatgagt tttgtattcc aggcccagaa cggcgcccct cggtattcgc
     3001 tggtggggtt tttaaaaagc tctttcgata gttcatttaa tttcctcgga tataacacta
     3061 atatgccacc ttctttccta aaccattcat ttacttttta attcaaaata gtactggcca
     3121 ctgcctacat ttacaattat gctatcttct tcgatcccct cgaattttat tttgctttcc
     3181 ggtaagttat cctgcttttt actgctatcc accaacaaca caccagattc atttaacacg
     3241 tattcttccc ccaaccgggg tataaaaata ttggcctcgc agccaaaggg aatagctatc
     3301 gatagcttaa aagtagtttc cgccttttcc caggaaacca gaaattctcc ttttaccgtt
     3361 tttactttac cagaagcaaa acttacatcg gaaaccagat ggggttttat cttaacttct
     3421 tcccagccag gttttgtcaa cgacagtccc accaagtatt tataaaacca ggtatccacg
     3481 ctacccagca ttatatgatt ttgagaattc atgcctcctg cttctaactt ttcccatctt
     3541 tcccatactg tggtggctcc ttctttaatc atatatcccc agcccgggta gctttcttga
     3601 gttataattt tgaaagcaag gtctgcataa ccattttcac ttaagacatc aaagaggtat
     3661 ctggtcccca ctatacctgt atcaacatga gaatcctgat gctccactat attgtctatt
     3721 aatctttcta aaatgaattc ctttttatcc ttgggaacca tttcataata cagaggcaga
     3781 atttgagaag tttgagaaat taacctgtca accggtccaa acattctggt ttcatagccg
     3841 tattctttca ggaaacctcc attaaaacta ttctttattt tctcagataa ctctaagtaa
     3901 tattttgctt cgtcttcttc acccagaact tcgcaaattc tagaaaacag gtatacatcg
     3961 ttataatagt accagctcga ggtaacctca acaggggttt ttttaggaga tatggcacca
     4021 ggaggacacc agtcaccgaa tttgcccagc ttttttatta aattgttttc actgttatct
     4081 ttcaggaatt caacatattt tttcatagaa tcaaaatggt tcctcagaac ttgctcattt
     4141 ttatagtact catataaata ccaggctaaa gttatatatg cacttcccca ggcaggatcc
     4201 gcaggataaa tttttatata agcgggaacg acatctggaa tagaaccatc ttctttttga
     4261 gataatttta tttcctgtaa ataattttca taaaaaagat tcatatcaaa gttataaata
     4321 gcctcttcag cagaaagttg cgcatctccc atccatccaa atctttcatc tctttgagga
     4381 caatctgtag gaatactgtg aagattactt aactggcccc acaatatatt attgtgaact
     4441 ttatttataa gctcattgga gcagtaaaag tcaccgattt tttcaacatc agtgtgaaca
     4501 aaacagcctt ctacactttc aattgttgga actgttggaa atcccgatac ttctatatat
     4561 ctaaaaccat gataagtaaa tcttggctca taagtctcaa gaccttcacc cttgaggacg
     4621 taagtatcag tagcttctgc ttttcgaatt gtggccgtat ttaaagtacc atccttatta
     4681 atcaattcac tgtgtcttat ttttatttct gttccccttg gaccttttac tgctattttt
     4741 acccacccgg tgaagttttg cccaaaatcg tatatataca caccaggttt tatagaatac
     4801 atcttttgag gcttcagagc gctggtaact ctaataggag gcatgctttg atacctcaaa
     4861 tcaggaccac taactatttc tgtttcttcc cagttagtgt cgtcaaagtg gtagatattc
     4921 caattgggca tctctaatct ggcatcatat ttctccccgt aatatatccc attttcttgt
     4981 atagggccat gagaagattt ccaggaatta tcgctaaaga taaattcctt atttccatct
     5041 tcatattcaa ttaaaatttg caatactaat ctgggttttg aatagccata acgctcaata
     5101 tatctaccat ttcctaaaat cacgcctatg caatttttta gactaaggta attggttata
     5161 tcatatacgc tatatagtgc ttctttagaa tattcagtct gccctggatc gagaacattg
     5221 ttccctatct ttttcccatt caaatacagt tcataaaatc ccaatccgct aatataagcc
     5281 ctcgcctttt ttataccgtt tcttaactca aaaggttttc tgaaaaaaga tgcataatat
     5341 tgtttgtagc tctcagtatc ataatcaccg ctggatttaa atgtttttaa atccttttta
     5401 gcgatccatt gcgctttcca atcctttttc tctaacggtc cagtttcaaa acaagaggtg
     5461 ctactccagt cactcatacc tccctcttcg tcccacactc tcactttcca gtaatatcgc
     5521 ttaaaactct cgagttcctt cccctcataa taacaataat tttcgctgct ttctactttt
     5581 tgtgtatccc acatatctcc cttgttttct ttcattaaat ctggaaatga agaaaccaaa
     5641 agctgaaagg cagtttgttt gcctcttttt ttatcagaat caataatcca taaaaacttt
     5701 ggtttcccat tttttaacgc cagcggagtt gtggagtaat tacaatacat atctctgata
     5761 gaaatggtca ttttttttaa ctccttattg ttttttcttg ctcatttaat ctttattaac
     5821 ttcagatcta tcatttctaa aattgagaaa aaccctttgt taatagaaat ctttaaagtt
     5881 aagaatcaac catggaggta tgcaatataa acttgcaagt acaaatttta ggttgaaatc
     5941 ctttcaggtt gcaaagctat tctactttcg cccgggcaaa aaaacccctg tctttaccaa
     6001 ctacatatgc tgtgttttca atctcgaaag tatcactcaa acgtatatct tcagaagatc
     6061 caccaaccat aactgtcatc gttcccgctt caacaatcca attcatatca acatcaagga
     6121 aagcaaattg gtcagcacgc atggaaaaat gaacagtttt agtctctcct actttaagcg
     6181 caacccgctt aaaacctgca agttctttac tgggacgaag catgcttgca acttcgtctc
     6241 ttacatagat ttgtacaacc tcctctccat ctctaccacc aatatttgtt acctcacaag
     6301 aaaggttaat aacaccgtca gctttcactt ttttatctac ttttaaattt gaatattcaa
     6361 attgagtata gcttttgcct tctccaaaat aaaaaagtgg ctgtttagat ccttcagcat
     6421 acatattcat aaccattcct ttaacttcgt catagctatc cccttttctg tgcctggcat
     6481 aaattggaat ctggcctgcg tttctcgcta ccgtaactgg caaacgcccg gcaggattgt
     6541 aatcgccaaa tagcacatct gcaagggcac ttccacctgt gattccagga aaccaatttt
     6601 ctattattgc cggatagttt tttgcaatat actcgctgga aagaggtctt gcatccatat
     6661 gaacgaagat tgcaggagtc ccggtttcaa aaatagcctt tgctaactct tcctgtacac
     6721 cggggagacc aatacgatcg ctatcaatac cctcaccgat agtgcaagta ttaccccacc
     6781 catatttacc accaagtaca agaattacaa cgtccgctct ctttgctgca gctattgctg
     6841 cagcaaaccc accacgattt gttcccgcaa catcacagcc cctttcatac tctacgttag
     6901 cgtcaggaca ttttgcctta attgagtcaa gtatagttgg agttatggct ccatacaatt
     6961 tctttatcgt ttcttccaca attggtggct cttgtctaac attactgcct tcaaaaaacg
     7021 gtggtagtga ttgggtatct cctaccttat ttgtttctaa gtcactacct gccatatcca
     7081 gtgtccctgc catttcagac atagtttcag ttagcatcat ttccagcata gctggatagg
     7141 tataacagcc aaataatagc cggagagaat taccatgtgg gcctattact gcaatattct
     7201 ttatatcttt atttaaggga agtatgccat cattttttag aagcacgatg gattcacgtg
     7261 ccgcctttaa actatgggcg gtggctgctt tattttcata agattcagct attaaatcga
     7321 ctcttggata aggatcttca aatagaccca gctcaaattt tgtttttagt attctacgaa
     7381 cagaacaatc aattaatgat tcgtcaataa tcccttcatt aactgcttcg atcaaggtct
     7441 gtgtataacc ataaggaaaa ggcaactcga tatctaaccc cgcctgcagg gcttctatac
     7501 cagcccattt cggatctgga gatgctttca agtcgcatgc tcttttaata gacatataat
     7561 ctgaaacaac caggccatca aaacccattt cctgacgcag aaggtccgtt aatatatgtt
     7621 tggatttgat gaccatttcg ccgtcaattg tgccatagga attcataatt gactttaaac
     7681 caccttctgt gatagctgct tgaaagggtt ttgcatatac ctccctgagt tcacggggag
     7741 gaataggatt agatgacata ttcagaccac cttcacagaa accataacct aaaaaatgct
     7801 tcccggttgc cacaatgcca tttttaagct catcaccctg taatcccttt gtaaaagcaa
     7861 cactcatagc tgaacaaagc gtagggtctt caccataggt ctctcctatc ctgccccacc
     7921 gtggatcccg ggcaacatcc ataactggcg aaagtgcttg tctcacacca actgctagca
     7981 tctgcttacg gataatattt gtcatttttt ctactgtgtc aggattccat gttgccccca
     8041 atccaatcgc cgagggaaaa actgttgctc ctgcggccat taacccggta agtgcttcgc
     8101 tatgcaataa aacaggtatt ccaagatcgt ttttagcacg aataatatcc tgaacccttt
     8161 ttgctaactc tgcattagct tctatcgtct tccctattga tgtcgttcct gttacctctc
     8221 caataccttc tgggaatccc tgcaatccct ggtcgggatc actcataagc attgtacatt
     8281 gtagctgagc tactttttgt tgcagcgtca ttcgagagag caaatcttct actcgttcgc
     8341 ttattgaacg attcttatct ttataaatct ccataattat tcccctttca aatttattcg
     8401 caaactagac cggtacttcc attcgaagct ttcacttcta acattttggg ctatcaaaat
     8461 tttatttgtc cactcagtcc acgaaactac agtttatatc ttctaactac tgataattta
     8521 ctttatatca tttatcacct ccttcagctc tacgaatata tctttctagc gctgtaacaa
     8581 aaagtatggg agagcttttt tgatactcca caaaagttgc cttataaaca ttcgcaaata
     8641 aaactgcatt gcatttgaat caataataga ggcatatcat caaccttaca agggctactt
     8701 accaatttta atcttatttc agatttttca aaatcttgta acaagagcta attactcctt
     8761 tctctttcat aaagaaagca tctaactact ctaccttcca cataatattc ttctggattt
     8821 tctcttccgc atataccctc agcacaagaa catctttcag caaatttgca cccgggcaaa
     8881 ttgaattctt ccacttcaaa agaagctttt ttaactctac tttctctacg tttttttggt
     8941 attggttgta aaagggattc tttcaacaat ttagaataag gatgcaatgg ttttatt
//
LOCUS       DAOSVE010000072         8832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_4197_length_8832_cov_69.704455, whole genome shotgun sequence.
ACCESSION   DAOSVE010000072 DAOSVE010000000
VERSION     DAOSVE010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 8832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8832
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4197_length_8832_cov_69.704455"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            366..1706
                     /gene="xseA"
                     /locus_tag="PK016_06540"
     CDS             366..1706
                     /gene="xseA"
                     /locus_tag="PK016_06540"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HXL02754.1"
                     /translation="MENISQSSYLTLKELTQILKDVVEDYYFSAPLRIVAEIAEIRLN
                     STGHCYLELVEKEDDHVVARMKGIIWSSNYLLVASSFLEVTGKNLERGMKILFLARLR
                     FHPVHGLSLDIKEIDPSYSLGEMIRKRKEILERLEKEGFLTLNRSLSFPLFPQRIAVI
                     SSPTAAGWGDFISHLQNNPHGFSFFCKLFPTLMQGEEVENSLFSSLEEIRKEQNYFDV
                     VVIIRGGGSIVDLSCFDSYLLGKAVATFPLPVVVGIGHERDDTVLDYVAHTSLKTPTA
                     VAEFFVNQMKTVEEEIEEEMTELKEMVGSFIEKEKRVLTILVDQWTRRVRDLLLLSSK
                     ELSFCRERLLIRSQKILGEIGENQRVCERQLKNLVEERVSKERETLKRAEEAIRLLNP
                     ENVLRRGYSVTYREGKVVKSFRDLQEGEDISTCLGDGWIKSKVEETYGRGQLEL"
     gene            1681..1884
                     /gene="xseB"
                     /locus_tag="PK016_06545"
     CDS             1681..1884
                     /gene="xseB"
                     /locus_tag="PK016_06545"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HXL02755.1"
                     /translation="MEEDNLSYSEAFQELKSIVDELENEEIEIDNLTAKVKRASFLYR
                     FLRDRLRATEEEIKKVLEEEEPF"
     gene            complement(1897..2802)
                     /locus_tag="PK016_06550"
     CDS             complement(1897..2802)
                     /locus_tag="PK016_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HXL02756.1"
                     /translation="MEEKHEFLNSQKNEKALLIAIILNLIISLTEIIGGIWSGSLALL
                     SDALHNLGDTTSLFISLFSLRLSLKEETPQRTYGYRRAEVLGAFVNSLLIFATLGYIF
                     IEAIQRLFSPQVVEANIILGIAIVGLSGNLFSVFFLFPGSRESLNLRSSFWHLLADTF
                     SSLVVIIGALFIKYRGWFFIDPLFSIGLSLLLLRGSWKMFKEVVNILMQGAPPHLNLE
                     SLKKGLEALPGVKNIHHLHVWSLNDKEHFAEFHVTTTCSSWEEIDQLREKIQKTLFED
                     FQIEHSTIQFENKGCKNQDLIVKEK"
     gene            complement(2839..3237)
                     /locus_tag="PK016_06555"
     CDS             complement(2839..3237)
                     /locus_tag="PK016_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II SORL domain-containing protein"
                     /protein_id="HXL02757.1"
                     /translation="MANFSDLFKSADWKQEKHVPVIEGPDIVKKGELVNFSATVGKEI
                     PHPNTTEHHIRWIAIYFLPTGGKFPFQLGHFEFTAHGESVEGANQGPVYTEPRVNFSF
                     KTTQPGTIFVTSYCNIHGLWKGEKEIKVEE"
     gene            complement(3251..3763)
                     /locus_tag="PK016_06560"
     CDS             complement(3251..3763)
                     /locus_tag="PK016_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011012427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HXL02758.1"
                     /translation="MPIKHKMTQENLENAYAGESQAHMKYLIFSQVAEKEGKPNIARL
                     FKAIAYAEEVHATNHYRALGNINDTVKNLDMAIAGETYEVEEMYPAYNAVAKLQGEKE
                     AEITTHGALEAEKIHAQMYKKAKEIAEEGKDIALGTIQICSVCGYTTEGEAPEKCPIC
                     GAPREKFKAF"
     gene            4181..4666
                     /locus_tag="PK016_06565"
     CDS             4181..4666
                     /locus_tag="PK016_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02759.1"
                     /translation="MRTFRRYMVMGLVLFIAFFLGTLTENNPLSYSKEDMKSMAEKVG
                     LTAQAFYTAGSDQVYYQIEQNLVVLDEIIDMVEKTESIGAVINPMVEKLEKITISYEH
                     ISNLKPRIEEELRGKIGEFQELLKKSKDQIRILENEKLASHFPGIKLLRYRERRSKKR
                     V"
     gene            4676..4876
                     /locus_tag="PK016_06570"
     CDS             4676..4876
                     /locus_tag="PK016_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02760.1"
                     /translation="MVGSHLGNNFFLVLGSSKKWKWFSPPRSWSDIAVLIQEIEEKTQ
                     KLNQILQEALHSAKEKEAPLSP"
     gene            complement(4877..5914)
                     /locus_tag="PK016_06575"
     CDS             complement(4877..5914)
                     /locus_tag="PK016_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HXL02761.1"
                     /translation="MDLYLGVDVGSVTTKFVLMDKEKNILASCYLRTSGDPIGALKEG
                     LRYIERYFPSGGEIKGVGSTGSARHLAGIVVGADIVKNEITAHAIAAITRVPEVRTVF
                     EIGGQDSKLIIIRDGVVEDFAMNTVCAAGTGSFLEHQAVRLNVPIEEFGKLALRAEQA
                     VRIAGRCTVFAESDMIHKQQLGFGKPEIVKGLCEALVRNFINNLATGKTIEDPIVFQG
                     GVAANEGIVQAFQDFFQGKKRIIVPPEHGVMGAWGAAILALEYAPEKSAFSGFEVISR
                     QFENRSFTCEDCPNACEIVILKEEDDIKALWGSRCGKWTPATLKKRWEEKEHNPQEPL
                     QIEEEESLVKG"
     gene            complement(5915..7015)
                     /locus_tag="PK016_06580"
     CDS             complement(5915..7015)
                     /locus_tag="PK016_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02762.1"
                     /translation="MKATFPHLLHLDIALGVLYPTLGVEIVTPPPITQKTIEIGVKLA
                     PQNACFPLKVTLANFIEGIELGADTIFMAGGVGPCRFGYYGQIQNIILEDLGYNVNFV
                     LLDHPRYGIRPFIRALQTMAGGRVLSSRVIKVASLAWKILCFTEDLEKERRALRVQVK
                     DPQKLDEIVEGALEQLRQANNEENFQAIREETLKKLKSLKRKEKEDFIKVGIVGEIYI
                     AQETRINFNVEKILGDLGAYVHNSVSTVEYVLHTLPFTRSQEKLEAWKLAKPYLKRFV
                     GGEGIDTVGSTIRYAQWGFDGVVHLYPFTCMPEVVAKSILAAVSKDYDLPILHLAIDE
                     HSGEAGLVTRVEAFMDVLERRKKLKKRKLEVS"
     gene            complement(6981..7979)
                     /locus_tag="PK016_06585"
     CDS             complement(6981..7979)
                     /locus_tag="PK016_06585"
                     /inference="COORDINATES: protein motif:HMM:NF021504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HXL02763.1"
                     /translation="MKVGIPRGLLFYRFYALWKTFLEELGAEIVISSPTNKGIIQQGL
                     TYAVEEICFPAKVFLGHVLYLKDRVDLLFIPRVVSFHRKEYNCPKILALPDLARNLFS
                     IPEERIIDDEINMREEGIRGWRKGFLNLGKHFTTKESKIMKALRLAEKAHQDYQANLK
                     KGMLPEDLIDHKNSLPPREKKIVLLGHTYLLSDSYINMNVAKKVNDLSYSVITPDMVE
                     EEKRRYYLSTIPKPSFWTISNEIIGSALYHLKERNPEVRGFIHLVSFECGPDSLVGEL
                     IERLIKREKESLPYLRLEIDEHTGEAGLVTRLEAFVDMMEWRYKNFESYVSSFTSS"
     gene            complement(8054..8536)
                     /locus_tag="PK016_06590"
     CDS             complement(8054..8536)
                     /locus_tag="PK016_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXL02764.1"
                     /translation="MTETTIEKNLSHHRLKELIPELVSIYQRAYENLPEYREDSYWKI
                     VAYLHWLWRGDPEGFLVAFEGDKPAGFSGMHTNWREGKEYLAEIHELVVDPQFQEKGI
                     GKKLIEEATRIAISRGRKKIELWVGEKNYQAQEFYLHNGFQQRGQRGIWIRMIKELNL
                     "
BASE COUNT         2606 a         1734 c         1723 g         2769 t
ORIGIN      
        1 gttagtttgt tcgattcaat attctactct acttgtataa cctctcgaaa ggattttact
       61 gagggatact tttctccctc gatggttctt gctttttaac tctcttatta tctcggagga
      121 tttttgaagg ggtaataagg ggattttctc ccctctagtt ttggattctt aatacattca
      181 gggatgacaa gagaggatga tagatacatt cgagaggtag caaaaaataa ggaagttata
      241 ggagggttat ctccttattg ttgataatag gttttgcagc gactatattt gttaaattga
      301 atttgcaagt taaggattgt agaaaggttt tcttctggta aaattaaata aggaaggagg
      361 aagcggtgga aaatatttcc caatcgtctt atcttactct aaaagagcta actcagatat
      421 tgaaggatgt ggtggaagat tattactttt ctgcccctct ccggatagta gcagagattg
      481 ctgagataag attgaactct actgggcact gttatctgga gctggtagaa aaagaggatg
      541 accatgtggt ggctcgaatg aaaggaatta tctggtcttc caattatctt ctagtagctt
      601 cctcttttct ggaagtaaca ggtaaaaatc tggaacgggg aatgaaaatt ctctttctgg
      661 ctcgtttgcg atttcatcct gttcatggtt tgagcctgga tattaaagaa atcgacccct
      721 cttattcctt gggagaaatg atacgtaaaa gaaaagaaat tttagaacgt ctggagaaag
      781 aagggttttt aactcttaat cgttctcttt cttttcctct ttttcctcaa cgaattgcag
      841 taatttcttc tcctactgca gcagggtggg gagactttat cagtcacctt cagaacaatc
      901 ctcatggttt tagttttttc tgtaagctat ttcccacttt gatgcaggga gaagaggtgg
      961 aaaattctct tttttcttct ctggaggaga taagaaaaga acagaattat tttgacgtag
     1021 tggtgataat caggggagga ggttctattg ttgatttgag ctgttttgat agttaccttt
     1081 taggaaaagc agtagccact tttccccttc cagtagtggt gggtattggt catgaacgag
     1141 atgatacggt tttagactat gtagctcata ccagcttgaa aacccccact gcggtagctg
     1201 aattttttgt taatcagatg aaaacggtgg aagaagaaat agaggaggaa atgacagagt
     1261 taaaggaaat ggtaggaagt tttatagaaa aagaaaagcg tgttttgaca attcttgttg
     1321 accagtggac cagaagagta agagatttgc ttcttttatc gagtaaagaa ctttcctttt
     1381 gtcgggaaag gttattaatc cgttctcaaa aaattttagg agaaataggg gaaaaccaga
     1441 gagtctgtga aaggcaatta aaaaatttgg tggaagaaag ggtttctaaa gagcgggaaa
     1501 cattgaaaag agctgaggag gcaatacgtt tgttaaatcc agaaaatgtt ctccgccggg
     1561 gttatagcgt aacttatcga gaaggtaagg tggtaaaaag ttttagagat ttacaggagg
     1621 gagaggatat ttctacctgt ctgggggacg gctggattaa gagtaaagtg gaggagactt
     1681 atggaagagg acaacttgag ctatagtgag gcttttcagg aactgaaatc tattgtggac
     1741 gaactggaga acgaagaaat agaaatagat aatttaacgg caaaagttaa aagagcttct
     1801 ttcttgtatc gttttttaag ggaccgtctc cgggctacgg aggaagaaat aaaaaaagtt
     1861 ctggaagaag aagaaccttt ttgagaaaac aaaaatttat ttttctttca caatgaggtc
     1921 ctgattttta caacctttgt tttcgaattg aatggtggaa tgttcaattt gaaaatcttc
     1981 aaaaagagtt ttttgtattt tttctcgaag ctggtcaatt tcttcccagc tggaacaggt
     2041 agtagttaca tggaattcgg caaaatgctc tttatcattt aaagaccaca catgaagatg
     2101 gtggatattt ttcactcccg gcaaagcttc caggcccttt tttaagcttt ctaaatttaa
     2161 gtgaggcggt gccccctgca ttaggatatt aaccacttct ttgaacattt tccagcttcc
     2221 ccgtaaaaga agaagagata gtcctatgct gaagagagga tcgataaaaa accatccccg
     2281 gtatttgata aacagagctc caattattac tactaaagaa gagaaagtgt ctgcaagaag
     2341 atgccagaaa gagcttcgaa gattaaggct ttctcgagaa ccaggaaaaa ggaagaaaac
     2401 agaaaataaa tttcctgaga gacctactat tgctattcct aaaatgatat tagcttctac
     2461 tacctgagga gagaacagcc tttgaatggc ttctataaaa atgtagccaa gagtagcgaa
     2521 aataagcaag gaattaacaa aagcacccag aacttctgcc cttcgatagc cataagttct
     2581 ttgaggagtt tcttctttta aagagaggcg gagagagaaa agacttatga aaagagaggt
     2641 agtatctcct aaattgtgca gagcatcgga aagaagggct aagcttcccg accatattcc
     2701 tcctatgatt tcagttagag aaataataag atttaagatt atggcaatta agagagcttt
     2761 ttcatttttc tgagaattaa ggaattcatg tttttcttcc ataagaagat atgaagaata
     2821 aagggggtgc tacccccttt attcttctac tttgatttcc ttttctcctt tccacaatcc
     2881 atgaatatta caataagaag tgacgaaaat ggttcccggt tgagtggttt tgaaggagaa
     2941 attcactcgt ggttcggtat acaccggtcc ctggttagca ccttcaacgc tttctccatg
     3001 agcggtgaat tcaaaatgtc ctaactgaaa gggaaattta cccccagtgg ggagaaagta
     3061 gatcgctatc caccggatgt ggtgttcggt agtgttagga tggggaatct cctttcccac
     3121 agtggcactg aagttaacca gttctccctt tttaactata tcaggtcctt cgattaccgg
     3181 aacatgtttt tcctgtttcc agtcagctga tttgaaaagg tcactaaaat tagccatttt
     3241 tttaacctcc ttagaaggct ttgaattttt ctcgaggagc tccacaaata ggacattttt
     3301 ctggagcttc tccttctgta gtatagccac acactgaaca tatttgaata gttcccagag
     3361 ctatatcttt accttcttct gcaatttcct tagctttttt gtacatttga gcatgaattt
     3421 tttctgcttc taatgctcca tgagtagtaa tctctgcttc tttttcgcct tgaagcttag
     3481 ctacagcgtt gtaagcagga tacatttcct ctacttcata tgtttctcca gcaatggcca
     3541 tatctaaatt ctttaccgta tcgttgatat tccctagtgc tcgatagtgg ttagtagcat
     3601 ggacttcctc agcataagca atagccttaa aaaggcgagc gatgttaggt tttccctctt
     3661 tttctgctac ctgagaaaaa attagatatt tcatatgagc ttgtgattca ccagcataag
     3721 cattttccag attttcttga gtcattttgt gcttgatagg catatgtggt tcaacctcct
     3781 ttttaattca ggatttttaa gaagtaaact aaatcggtag tattttattc cacaagggaa
     3841 tgtttaaaaa cagccgggta aaaaagggct aaaagcttcc cgctcctctg gtcttcacga
     3901 ccaatttcct tttacccggc tgatagatat tatacaactt ttaatatttt ttggaaattg
     3961 ctcatcagta atgcttttct cctctggttt tttccttatc gatttaggga ataataaaaa
     4021 gaagtacgtt tcgtttaaag ctatgacatt agcactacaa tatttgaata tagtattgaa
     4081 gattaaattt tatactttct ccgcaactct cctcttatta ctctatgttg ccttagtaag
     4141 tcagaaccgc tacgggagaa aggaggtgtt aaaataaata gtgcgaacat tcagacggta
     4201 catggtcatg ggtttggtgc tttttattgc tttctttctg ggaactttaa ccgagaataa
     4261 ccctctttct tattccaagg aggatatgaa gtcgatggct gaaaaagtgg gcttgactgc
     4321 tcaagctttt tacactgcag ggagtgacca ggtttactac cagattgagc agaatttagt
     4381 tgttctggac gaaattatag atatggtaga aaaaacagaa agtattggtg cggttataaa
     4441 ccctatggta gaaaaattag agaaaattac catctcttac gagcatattt ccaatcttaa
     4501 accccggata gaggaagaat taaggggaaa aataggagag tttcaagaat tacttaagaa
     4561 atcgaaagac cagattagga ttctggaaaa tgaaaaatta gcttcccact ttccaggaat
     4621 aaaattattg cgctatagag aaaggagaag taagaagagg gtttaggaaa atagaatggt
     4681 gggtagtcat cttggtaata atttttttct tgttctggga agcagtaaaa aatggaaatg
     4741 gttttcccct cctcgtagct ggtctgatat agccgtgctt atccaggaaa ttgaagagaa
     4801 aactcaaaaa ttaaaccaga ttttacagga ggctctccac tcggcaaaag aaaaagaagc
     4861 tcctttatca ccatagttac ccttttacta aagattcttc ctcttctatc tggagaggtt
     4921 cctggggatt atgttctttt tcttcccatc tctttttcag tgtggcagga gtccacttac
     4981 cacaacgact accccataaa gccttaatgt cgtcttcctc ttttaatatt actatttcac
     5041 aggcattggg acagtcctcg caggtgaagc tccggttctc aaactgacgg ctaattactt
     5101 caaatccgga aaaagcactt ttttctggag catactccag agctaaaatg gctgctcccc
     5161 aggctcccat tactccatgt tcaggaggaa cgatgattct ctttttaccc tggaaaaaat
     5221 cctgaaaagc ttgcacaatt ccctcattag cagctactcc tccttgaaaa acgatagggt
     5281 cttctatggt ttttcctgtg gctaagttat tgatgaaatt tcgcaccaaa gcctcacaga
     5341 gtcctttaac tatttctggt ttgccaaaac ctaattgttg tttgtgaatc atatctgatt
     5401 cagcaaaaac cgtgcatctt ccagcaatac gtactgcctg ttcagcccgg agagccaatt
     5461 ttccaaattc ctcaataggg acgttgaggc gtactgcttg atgctctaag aaagaccctg
     5521 ttccggctgc gcaaacggta ttcatagcga agtcctctac cacgccatcc cggatgataa
     5581 ttaacttgct atcctgccct ccaatctcaa aaactgtacg tacttcaggt actcgagtaa
     5641 tagctgctat ggcgtgagca gtaatttcgt ttttaactat atctgctcct accactattc
     5701 ctgctagatg ccgagcacta ccggtactac ctactccttt tatttctcca ccactgggga
     5761 aatatctttc tatatagcgt aaaccttctt tcagcgctcc aatagggtca ccactggtgc
     5821 gtaagtaaca actggccagg atattttttt ctttgtccat aagtacgaac ttagtggtta
     5881 ccgaccccac atccactcct aaataaaggt ccatttaact aacctccagc tttcttttct
     5941 tcaatttttt tcttctttcc agaacatcca tgaaggcttc tacccgggta actaatcctg
     6001 cctctccaga atgttcgtct atggctaaat gcagaatggg taagtcataa tccttactta
     6061 ctgcagcgag tatactttta gctactactt caggcatgca agtgaaagga taaaggtgga
     6121 caactccatc aaatccccat tgagcatagc gaatggtgct acctaccgta tcaatccctt
     6181 ctcctcccac aaatctcttc aggtaaggtt tggctaactt ccaggcttct aatttttcct
     6241 gagagcgagt aaaaggtaga gtatgcagga catattctac tgttgaaaca gagttgtgca
     6301 catatgctcc caggtctcct aggatttttt ccacattaaa atttattctg gtctcctgag
     6361 ctatataaat ttctcctaca atccctactt ttataaagtc ttccttctct tttcgcttta
     6421 aactttttaa tttcttcaga gtttcttccc ggatagcttg aaaattttct tcattgtttg
     6481 cctggcgaag ttgttccagt gctccttcta ctatttcgtc taatttttga gggtctttta
     6541 cctgaacacg cagggctctt ctttcttttt ctaagtcttc ggtgaagcac aaaattttcc
     6601 aagctaaact tgctacttta ataactcgag aagaaagaac ccgtcctcca gccatagttt
     6661 gtaaagccct tataaagggg cgaatgccat agcgaggatg gtctaataaa acgaaattaa
     6721 cattatatcc taaatcctcc agaattatat tctggatttg gccataataa ccaaatcggc
     6781 aaggtcctac ccccccagcc ataaaaatgg tatcagctcc cagctcaatt ccttctataa
     6841 aattagcaag agtaactttg aggggaaaac aagcattttg aggagctaat tttaccccga
     6901 tttcaatagt tttttgagta atgggaggag gggttacaat ctctactcct aaagtggggt
     6961 agagaactcc tagagcgata tcaagatgaa gtaaatgagg aaacgtagct ttcaaagttc
     7021 ttatacctcc actccatcat atctacaaaa gcttctaatc gagtgactaa acctgcttct
     7081 ccagtgtgtt catcaatttc caaacgcaaa taagggagag actccttttc ccttttaata
     7141 aggcgttcga ttagttctcc cactaaagag tctggcccgc attcaaagga aactagatgg
     7201 atgaatcctc taacctccgg atttctctct tttagatggt ataaagcact accgatgatt
     7261 tcattagaaa tagtccagaa gctgggttta ggtatggtag agagatagta tcttctcttt
     7321 tcttcttcta ccatatcagg agtgataacc gagtaactga ggtcgttaac tttcttggca
     7381 acattcatat ttatataact atcacttaga agataggtgt gaccgagaag aactatcttc
     7441 ttttcccggg ggggaagaga atttttgtgg tcaatcaaat cttcagggag catccctttt
     7501 ttcaggttgg cctgataatc ctggtgggct ttttctgcta atctcagcgc tttcatgatt
     7561 ttgctttctt tagtggtgaa atgtttacca aggtttaaaa agccttttcg ccagcctcgg
     7621 attccctctt cccgcatatt aatttcatca tcaattattc tttcttcagg aatactaaaa
     7681 agattacggg ctaagtcagg gagagctaat attttaggac agttatattc ttttctgtgg
     7741 aaactgacta cccggggaat aaaaagtaaa tccacccggt ctttaagata aagaacatgt
     7801 cctaaaaata ctttggccgg gaaacaaatt tcctccaccg cataggttaa accttgctgg
     7861 atgatgcctt tattagtagg ggaggaaatt actatttctg cccctaattc ttccagaaaa
     7921 gttttccaga gagcataaaa acggtaaaat aaaagacctc gcggaatacc tactttcatt
     7981 ttttcacctg ctttaaaaaa catctaggtt ttattataca acttaaaccc caaaaaacaa
     8041 aaaaacttcc tttctacaaa tttaattctt ttatcattct aatccatatt cctctctgtc
     8101 ctctttgctg aaacccgtta tggagataaa actcctgagc ttggtaattt ttttctccta
     8161 cccataactc tattttcttt ctccctcgag aaatggcaat ccgagttgct tcttctatca
     8221 atttttttcc tatccctttt tcctgaaatt gagggtctac tacaagttcg tgaatttcag
     8281 ccaggtactc ttttccttct ctccagttag tgtgcattcc tgagaatcct gccggtttat
     8341 ctccctcaaa agccactaaa aacccctcag ggtctcccct ccatagccaa tggagataag
     8401 ccactatttt ccagtagctg tcctctcgat actcagggag attttcataa gctcgctgat
     8461 aaatggaaac aagctcggga attaattctt taagtcgatg atgactcagg tttttctcaa
     8521 ttgttgtttc cgtcaattgt ctcccccttc tctcgaaatc aaaaatggat tttctttgtt
     8581 gtttttacta attatatagt taaccgcaaa atccattaca aagactagag ggggaaaaag
     8641 acccccccct cataaccccg aactataaag gggctttgtc cctttataat cccccaaaaa
     8701 gcaaaagaaa aagacaaaag actgaaaaga caaaaactac tgaggaagga gaaaccttcc
     8761 tcagtgcctg cggcactaaa gacgaaagca caatagatga aagaaactaa aaccaattac
     8821 gctggaaaaa ag
//
LOCUS       DAOSVE010000073         8604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_4304_length_8604_cov_69.806410, whole genome shotgun sequence.
ACCESSION   DAOSVE010000073 DAOSVE010000000
VERSION     DAOSVE010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 8604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8604
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4304_length_8604_cov_69.806410"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..1660
                     /gene="groL"
                     /locus_tag="PK016_06595"
     CDS             80..1660
                     /gene="groL"
                     /locus_tag="PK016_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049018.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HXL02765.1"
                     /translation="MAKEIIKDIKAREALGKGVDILAGVVRITLGPKGRNVLLEKKYG
                     SPVVTNDGVTIAREIEVEEPEENVGVQVAKEIATKTNDLVGDGTTTALVLAQAMLREG
                     LKSVAFGHNPVFFRRGMEEALRRTLEKIKSYSLAVEDKKAISQVASISANDPVVGEIV
                     AQALEKVGKDGVVTVEESKSSETTIEVTEGMQFDQGYLSSYFVTNSERMEVILEEPLL
                     LICDFKIANVVSLLPLLQKVFEMDRPLLIIAEDIEQEVLATLAVNKLQGVLKVAVVKA
                     PAFGDRRKEILGDLAVLTGGQVISQDIGMKLEKVTPDLLGEADKVRITKDSTTIIGGR
                     GNKKDIEARERQIRAQIEKTTSTYDREKLQERLAKLQGGVAIIWVGASSEVELKEKKY
                     RVEDAISAAKAAIEEGVVPGGGALFVHVAQELDFSDLREEERQGAEALRKALEAPAYW
                     IAENAGYDGSLVVEEIKKSSWRVGFEAQKGEFVDLVEEGVIDPTKVLRVALSNAESIV
                     SLILVSEALIFEAPEEKI"
     gene            1716..2879
                     /locus_tag="PK016_06600"
     CDS             1716..2879
                     /locus_tag="PK016_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HXL02766.1"
                     /translation="MKNWFRSDKGLLRTTYGFDDVSLVPGEITIDPQEVDVTTTIADE
                     KLNIPVLGAAMDGVIDPRMAIELGHLGGWGVLNLEGLFTRYDDPYSIIEEMIQKPREK
                     VTDFIQEVYRSPIKERLVGERIQEIKKGGVKAVASLTPPRVEALGKVAQEAGLDVLVI
                     QSTVTTLRHCSSTSRVLDLEKFCRETPIPVIVGNCATYEVALELMKAGAAGILVGIGP
                     GAACTTRAVLGIGVPQVTATWDAARARDTYLKATGKYVAVITDGGMRVGGDVAKAIAS
                     GADAVMLGSPLASAQEAPGKGHHWGMATPDPNLPRGTLVKVGIKGSLKEILLGPSHLT
                     DGTMNLIGALRSSMGSLGAKTIPEMQEVEIVVAPSIWTEGKSLQRTQGVGMGK"
     gene            2883..4418
                     /gene="guaA"
                     /locus_tag="PK016_06605"
     CDS             2883..4418
                     /gene="guaA"
                     /locus_tag="PK016_06605"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HXL02767.1"
                     /translation="MDKIVILDFGSQYTQLIARRVRECQVYCEIWPYHLKGYDLDSPE
                     IKGIILSGSPYSVGDANAPSLPPQILESGKPILGICYGMQLLVSTQGGKVVHAHGREY
                     GKALLEVSENRGIFENLAGDIVCWMSHGDSVKELPPGFRGVARTENCEYAAIRDEAGK
                     IWGIQFHPEVSHTPLGREILSNFLFRVCGCRPEWTPESIAKQQIQWIRNEVKDKKVVC
                     ALSGGVDSVTAAVLTYRAIGEQLTCIFVNNGLLRKGEAEQVFSKMKEILGKGRVIYRD
                     AAERFLSALQGVLDPEEKRKIIGRTFVEVFEEEAKKLGDVSYLLQGTTYPDVIESISV
                     YGPSVRIKTHHNVGGLPEHMQLKVIEPLRFLFKDEVRKLALELDIPEEIVWRQPFPGP
                     GLAVRIIGEVTEDRLQILRDMDFIIDQELRKSPIYKKLWQSFGVLVPVRSVGVMGDER
                     TYKYVGVLRAVMSEDGMTADWARISYDTMDAIARRVVNEVAGIGRMVYDITSKPPATI
                     EWE"
     gene            4431..5717
                     /locus_tag="PK016_06610"
     CDS             4431..5717
                     /locus_tag="PK016_06610"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019224918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HXL02768.1"
                     /translation="MAVIIVVGTHWGDEGKGKIVDYLGENAQVVVRAQGGNNAGHTVV
                     VGGEKYVFHLLPSGVLHPDKVGILGDGMVVDPSSFLGEIDFLRQKGKDVSGRLFLSPK
                     AHLVLPYHRILDGIYENIRGGGKLGTTGRGIGPAYEDKVARWGIRVGDLEDEKLFARK
                     LRVVLDYKNLLLERAFSQSPLPYDKILEDYLGYASLLKPYIADTSKMVYQFYREGKTI
                     LIEGAQGTMLDLDHGTYPFVTSSYPVAAGSLLGVGLGPVSNLEVLGVCKAYTSRVGEG
                     PFPTELRGETGDYLRKKGGEYGATTGRPRRCGWLDGVVLRYAARINGITSLALTKLDV
                     LGGLSSLKICRAYSIEGEEVDDFPVSPHLWERCEPIYEEMEGWEEDISSVSSYSDLPS
                     AARHFVEFVEDFVQIPIAIVSLGSGRENTLVRKEIF"
     gene            5727..7733
                     /gene="ligA"
                     /locus_tag="PK016_06615"
     CDS             5727..7733
                     /gene="ligA"
                     /locus_tag="PK016_06615"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HXL02769.1"
                     /translation="MEDIEKVKQEIENLRAIIRHHDYCYYVLNNPEISDEEYDALFKK
                     LQELEAKYPELITPDSPTQRVAGQPQEGFATFLHYRPLLSLSNAFTPEEVRDFDKRVK
                     RMLKAESLDYVVELKIDGLAVNLRYENGIFVRGATRGDGVVGEDVTLNLKTIHSIPLR
                     LRGDRIPRVIEVQGEAYMRKKDLERLNEERKKEGEPLFANTRNAAAGSLRQLDPRITA
                     QRKLDIFNYGAFVIDSDYEPSTHWELLNYLKELGFKINPHIQLISDIEEAIRIHEKWE
                     KERKNLDYDIDGLVIKVNSLSYHEMLGATSRSPRWAIAYKFESTYAITQVLDIEVNVG
                     RTGILTPVAVLEPVNVGGAIVKRATLHNEDEIKRKDVRIGDWVMVARAGEVIPEIAGV
                     LKERRMGEEKVFVMPRECPVCHSRAMREEGEVAWRCINASCPAQIKERIRHWASRDAM
                     DIEGLGEKLIDQLVEGGWVKTIPDLYRLNKETLLQLERMGNKSADNLLRAIERSKRRE
                     LPRFIYALGIPHVGQFIARLLVEQFGSLDKLKEAKEEELLAIEGIGPTVAESVRQFFS
                     NPQNRHMLEELKSLGVGPEELKKKEEGPLAGKTFVFTGTLSNFSRDEARALVEERGGR
                     AADTVSSRVDYVVVGENPGSKLAVAREKGIKILTEEEFYQLLGI"
     gene            7750..8076
                     /gene="gatC"
                     /locus_tag="PK016_06620"
     CDS             7750..8076
                     /gene="gatC"
                     /locus_tag="PK016_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HXL02770.1"
                     /translation="MKEVISLQELKRIAQLSKLEFSEEEFQGFFKDINNIAAHFEKLN
                     ELDLQDVSPTSHLSWSEPPFFSDEPRPGEGREEVLKVAPWVAGGYFVVPRIVDKEEKR
                     NEDRRV"
     gene            8057..>8604
                     /locus_tag="PK016_06625"
     CDS             8057..>8604
                     /locus_tag="PK016_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXL02771.1"
                     /translation="MKIEEFRDFTIADYHHLFKEKKISPLELVNLFLERIKREEDRIH
                     AFLFVNEEEAKSEAQKKAENPPDEFPPLWGIPIAIKDNICTRGIPTTCGSKILENFTP
                     PYDATVIERLKKAGAIILGKTNMDEFAMGSSTENSAFGPTYNPFDRRRVPGGSSGGSA
                     ASVCSLEAPLALGSDTGGSVRQP"
BASE COUNT         2510 a         1447 c         2358 g         2289 t
ORIGIN      
        1 ttttgggggt gataggggga gtctttttcc ccctatagtc tgttataatc ttttatatta
       61 agtaaggaag gtgaagtaat tggctaaaga gataataaaa gatataaagg caagagaagc
      121 tctggggaaa ggagtagata ttttagccgg tgttgtcagg attactctgg gtcctaaagg
      181 ccggaatgtt cttctggaga agaaatatgg ctcgcctgtt gttacaaatg atggagtgac
      241 cattgcccga gaaatagagg tggaagaacc agaggaaaat gtgggggtgc aggtggctaa
      301 agaaatagct accaagacca atgatttagt gggagatgga actactactg ctttagtttt
      361 agcgcaggca atgctccggg aagggttaaa aagtgtagct tttggccata atccggtttt
      421 tttccgccgg ggaatggaag aagcactgag aagaacccta gagaaaatta aaagttatag
      481 cttagcggtg gaggataaaa aagctatttc tcaggtagct tctatctcag ctaatgaccc
      541 ggtagtggga gaaatagtgg ctcaggcttt agagaaagtg ggaaaagacg gagtggtaac
      601 tgtggaagaa tctaaatcat ccgagaccac tattgaggta acagaaggta tgcagtttga
      661 tcagggttat ctttcttcat actttgttac caatagcgag cggatggaag tgattttaga
      721 agaacctctc ctgctcattt gtgatttcaa aatcgctaat gttgtctctc ttcttcctct
      781 tttacagaaa gtgtttgaga tggataggcc tctccttata attgctgagg atatagagca
      841 ggaagtatta gctaccctgg cggtaaataa attgcagggg gtgctaaaag tggcagtagt
      901 taaagcccct gcttttggag atagaagaaa agagatttta ggagatctag ccgttttaac
      961 tggagggcag gtaatctctc aagatatagg tatgaagtta gaaaaggtaa ccccggatct
     1021 actgggagaa gcggataaag tgcgtataac taaagatagc accactatta ttggagggcg
     1081 aggaaataaa aaagatatcg aagcccggga aagacaaatt agagcccaga tagaaaaaac
     1141 tacttctact tatgaccggg aaaagttgca ggaaaggctg gcaaaactcc agggaggagt
     1201 ggctatcatc tgggtgggtg cctcaagcga agtagagtta aaggagaaaa agtatcgggt
     1261 ggaagatgcc ataagtgctg ctaaagcggc gatagaggaa ggtgtagtcc cgggaggtgg
     1321 agcacttttt gtgcatgtag cccaggagtt agattttagc gacttaagag aagaagaaag
     1381 gcagggagcc gaagctttaa ggaaagctct ggaagcgcca gcttactgga ttgctgaaaa
     1441 tgcaggttac gatggttctc tggtagtaga agaaataaaa aagagtagct ggagagtggg
     1501 atttgaagct caaaagggag agtttgttga tttagtggaa gaaggagtaa tagacccgac
     1561 taaagtgctg cgagtggcat tgtccaacgc tgagagtatt gtttctctaa ttttagtttc
     1621 tgaggctctg atatttgaag caccggaaga gaaaatttga gattgagcaa gggtaatagt
     1681 ataattttgg acaaaacggg agaaaggtga gtagaatgaa aaactggttt agaagtgata
     1741 aaggtctttt gcgtactact tacggatttg atgatgtttc tctggttccg ggagaaatta
     1801 ctatagaccc tcaagaagtg gatgtaacta ccactattgc tgatgagaag ttgaacatcc
     1861 ctgttttagg tgccgctatg gatggagtga tagaccctcg aatggcaata gaactggggc
     1921 atttaggagg atggggagtt ttgaacctgg aagggttatt cacccgctat gatgatcctt
     1981 attccattat tgaagagatg atacaaaagc cccgggaaaa agttaccgat tttatccagg
     2041 aagtttatcg gtctcctata aaagaacgct tggtgggaga gagaatacag gaaataaaaa
     2101 agggaggagt taaagcggta gcttctctta ctcctcctcg ggtggaagct ttaggtaaag
     2161 tagcacaaga agcaggctta gatgttttag taatccagtc tacggtgact accctccgac
     2221 attgctcttc tacttcccgg gttttagatt tagaaaagtt ttgtcgggaa actcctattc
     2281 ctgtaattgt ggggaactgt gctacttatg aagtggcgtt agagttaatg aaagcgggag
     2341 cagcgggaat attggtgggc ataggtcccg gggcggcttg caccacccgg gcagttttag
     2401 gtataggtgt gccgcaggtt actgctacct gggatgctgc tcgggcgagg gatacttatt
     2461 taaaggctac aggtaaatat gtggcggtga ttaccgatgg aggaatgaga gtgggaggag
     2521 atgtggctaa agctatcgcg agtggtgcag atgctgtgat gttagggtct cctttagctt
     2581 ctgctcagga ggcaccgggg aaaggacatc actggggcat ggccactcct gaccctaatc
     2641 ttcctcgagg aactctggtt aaggtaggaa taaaaggaag tttgaaagaa attctcttag
     2701 gtcctagcca ccttaccgat ggtactatga atttgatagg agctttacgg tcttcgatgg
     2761 gttctttagg agcaaaaact atcccggaga tgcaggaagt ggaaattgta gttgctccca
     2821 gcatctggac ggaaggaaaa tctttgcaga gaacccaggg agtaggaatg ggaaaataga
     2881 taatggataa aatagttatt ctggattttg gttctcaata cactcagttg attgcccgaa
     2941 gagttagaga atgtcaggtt tactgtgaga tatggcctta ccatcttaaa ggatatgatt
     3001 tagacagtcc ggagattaaa gggattattc tctcaggtag tccctacagc gtaggagatg
     3061 ccaatgctcc gtcgcttcct ccccagattt tagaaagtgg caaacccatt ttaggtattt
     3121 gttacgggat gcaactttta gtcagcactc aaggggggaa agtggtgcat gctcacggtc
     3181 gggagtatgg taaggctctg cttgaggttt ccgaaaaccg ggggattttc gaaaatctgg
     3241 ctggggacat agtttgctgg atgagccatg gggatagcgt taaagaactt cctcccgggt
     3301 tccggggtgt agcccgaacg gaaaattgtg aatatgcggc gataagagat gaggcaggga
     3361 agatatgggg tattcaattt caccctgaag tctctcatac ccctttaggc cgggagatat
     3421 tgtctaactt tctctttcga gtttgtggct gtcgaccgga atggactcct gaatccattg
     3481 ccaaacagca aattcagtgg atccgcaacg aagtaaaaga taaaaaagtg gtgtgtgctt
     3541 taagtggagg agtagattcg gttactgccg cagttttgac ttatcgagcc atcggggagc
     3601 agctgacctg catttttgtg aataatgggc tgcttcgcaa aggtgaagca gaacaggttt
     3661 tttccaagat gaaggaaatt ttagggaaag ggcgagtaat ttatcgagat gctgcagagc
     3721 gtttcctttc tgctctccag ggtgttttag acccggaaga aaaaagaaag atcatcggta
     3781 gaacctttgt ggaagtattt gaagaggaag caaaaaaatt aggggatgtg tcttatcttt
     3841 tgcagggaac tacttatcct gatgttatag aaagcatctc cgtttatggt ccatcggtca
     3901 ggataaaaac tcatcacaac gtaggaggac tacctgagca tatgcaactc aaggtgatag
     3961 agcctcttcg ttttcttttt aaagatgaag tgagaaaatt agctctggag ctggatattc
     4021 ctgaggaaat agtctggaga cagccttttc ccggtccggg gttagcggtg agaatcattg
     4081 gtgaggttac cgaagaccga ctgcaaattt tgcgggatat ggatttcatc atcgaccagg
     4141 aactccgaaa atctcctatt tacaaaaagt tgtggcaatc ttttggagta ttggtgccgg
     4201 tgaggagtgt gggagtgatg ggagatgaaa gaacctataa atatgtggga gtgctgcgag
     4261 cggtgatgag tgaagatgga atgactgctg attgggcgag gatttcctat gatactatgg
     4321 atgccatcgc tcggcgggta gtgaacgaag tggcggggat tggaagaatg gtgtacgata
     4381 ttacttccaa gcctcctgct accattgaat gggaataaga ggagaagaaa atggcagtta
     4441 ttatagttgt aggaactcac tggggtgatg aagggaaagg taaaatagtt gattacctgg
     4501 gagaaaatgc ccaggtggta gtaagagctc aaggaggcaa taatgctgga cacacagtgg
     4561 tagtaggagg agaaaaatat gtttttcatc ttctcccttc tggtgttctt catcctgata
     4621 aagtagggat attaggagac gggatggttg ttgacccttc ttctttttta ggagaaattg
     4681 actttttgcg tcagaaaggg aaggatgttt cggggagact atttttgagc cctaaagctc
     4741 atctggtttt accttatcat cgaattttag acggaattta cgaaaatatt cgaggtgggg
     4801 gaaaactggg aaccacggga agaggtattg gtccggctta tgaagacaaa gtggctcggt
     4861 ggggaataag agtaggagat ttagaagacg agaaactttt tgcccgaaaa ctgagagtgg
     4921 ttctggatta caaaaatctt ttgttagagc gagcttttag ccaatctcct cttccttatg
     4981 ataaaatttt agaagattat ttaggttatg cttctctttt aaagccttat atcgctgata
     5041 cttccaagat ggtttaccag ttttaccgag aagggaaaac catcctcatc gaaggggcgc
     5101 agggcaccat gctggattta gaccatggta cttatccctt tgttacctct tcttaccctg
     5161 tggcggcagg gtcgctactg ggggtagggc tggggccggt ttccaatcta gaagtgctgg
     5221 gagtatgtaa ggcttacact tctcgcgtgg gagaaggacc attccctacg gagctccggg
     5281 gagaaactgg agattatctg cgaaaaaaag gaggagaata cggcgctacc actggtcgtc
     5341 ctcgtcgttg tgggtggctg gatggggtgg ttttgcgtta tgccgcgagg ataaatggca
     5401 taacctcttt agctttgact aaactggatg ttttgggagg gctgagttct ctcaaaatat
     5461 gtcgggctta ttcaatagaa ggagaagaag tggatgattt tcccgtaagt cctcacttgt
     5521 gggaaagatg tgagccgatt tatgaagaga tggaaggatg ggaagaggat atttcttcgg
     5581 tttcttctta ttctgacctt ccctctgcag cccggcactt cgtggagttc gttgaagact
     5641 ttgttcaaat tcccatcgct atagtttcac tgggaagtgg gcgagagaac actctggtga
     5701 gaaaggagat tttttaacca ccatttatgg aagatataga aaaagtaaag caggagattg
     5761 agaatctccg tgctatcatt cgtcaccatg attattgtta ttatgttttg aataatccag
     5821 aaataagtga tgaggaatat gatgctctat tcaagaaact ccaggaactc gaagcaaagt
     5881 atcccgagct catcactccc gattctccca ctcagagagt ggccggccag cctcaggaag
     5941 gttttgccac tttcctgcat tatcgacctc ttctcagttt gagtaatgct ttcacccccg
     6001 aagaagtgcg agatttcgat aaacgggtaa aaaggatgct caaagcggag agtctggatt
     6061 atgtggtaga gcttaaaatt gatggcctgg ctgttaatct gcgctatgaa aatgggatat
     6121 tcgttcgggg agctacccgg ggagatggag tggtgggaga agatgttact ctcaatctta
     6181 aaaccattca cagtatcccc cttcgcttgc gaggggatag aattccccgg gtgatagaag
     6241 tgcaagggga agcatacatg agaaaaaaag atttagaaag attaaatgaa gaaaggaaaa
     6301 aagagggaga gcctcttttt gctaacaccc gtaatgcagc agccgggtct ttacgacaat
     6361 tagacccccg gataacggca cagaggaagc tggatatttt taattatggt gcttttgtga
     6421 ttgatagcga ctatgagcct tccactcact gggaactgtt gaactatctc aaagagctgg
     6481 gattcaaaat taatcctcat atccagctga tttctgatat cgaggaagcc atccgtattc
     6541 atgaaaaatg ggaaaaggag agaaaaaact tagattatga tattgatggt ctggtgataa
     6601 aagtaaattc tctatcttac cacgagatgt taggagcaac cagcagaagt cctcgatggg
     6661 ctatagctta taaatttgag tctacttatg caataactca agttctggat attgaagtaa
     6721 atgtgggccg gacagggatt ctcacccctg tagcggtact ggagcccgta aatgtgggag
     6781 gagcgatagt aaagagagct actttacaca atgaagatga aatcaaaaga aaagatgtga
     6841 ggattggtga ctgggtgatg gtagctagag caggagaagt aatccccgaa atcgctggag
     6901 tattaaagga gagaaggatg ggggaagaaa aagtttttgt tatgcctcga gagtgtccgg
     6961 tctgtcattc ccgggcgatg agagaggaag gagaagtagc ctggcggtgc atcaatgcca
     7021 gctgtccggc tcaaatcaaa gaaagaattc gtcattgggc ttcccgggat gctatggata
     7081 tcgagggtct gggagagaaa ttgattgacc agttagtaga gggaggatgg gttaaaacca
     7141 tacctgacct ttaccgttta aataaagaaa ctcttttaca actggagagg atgggaaata
     7201 aatcagctga taacttgctg cgagctatag agaggtctaa aagaagagag ttacctcgtt
     7261 tcatctatgc tttagggatt ccccatgtgg ggcaattcat tgcccggctt ttagtcgagc
     7321 aatttggttc tttggataag ctaaaagagg caaaagaaga ggagcttctg gctatagaag
     7381 gtattggccc tacagttgca gaatcagtaa ggcaattttt ttctaaccct caaaatcgcc
     7441 acatgttaga ggaactaaag agtttaggag tagggccgga agagttaaaa aagaaagaag
     7501 aagggcctct ggcaggaaaa acttttgttt tcaccggtac tctatctaac ttttctcgag
     7561 acgaagctcg ggctcttgtg gaagagagag gaggaagagc tgccgatacg gtttcttctc
     7621 gagtggatta tgtagtggtg ggagaaaatc cggggagtaa gctagctgtg gctcgagaaa
     7681 aagggataaa aattctcacc gaggaagaat tttatcagct actgggaatt taaagaggga
     7741 ggtggtaaaa tgaaggaagt gatttccctt caagaattga aaaggatagc gcagctttct
     7801 aaactggaat tttccgaaga agagtttcaa ggttttttta aagatattaa taatatcgct
     7861 gcccattttg aaaagttaaa cgaacttgac ctgcaggatg tttctcctac ttcccatctt
     7921 tcctggagtg agcctccttt cttttctgat gagcctcggc ctggagaagg tagagaagaa
     7981 gtgctgaaag tagctccatg ggtggcaggt ggttatttcg tggtgccccg gatagtagat
     8041 aaagaggaga aaagaaatga agatagaaga gtttagagat tttaccattg cagattatca
     8101 ccatcttttt aaggagaaaa agatttctcc tttagagctg gtcaatctct ttttagaaag
     8161 aataaaaaga gaggaagata gaattcatgc ctttttattc gtaaatgaag aggaagcgaa
     8221 gagcgaggca cagaaaaaag ctgaaaaccc ccctgatgag tttccccctc tgtgggggat
     8281 tccgatagct attaaagata atatttgcac tcgaggtata cccactacct gtggttctaa
     8341 aatactggaa aacttcactc ctccctatga tgctacagta atagagaggc ttaaaaaagc
     8401 cggggctata atactgggta aaaccaatat ggatgaattt gccatgggtt cttctactga
     8461 aaacagtgct tttggcccta cttataatcc ttttgaccga agaagagttc cgggaggctc
     8521 gagtggtggt tcagcagcca gtgtttgttc tttggaggct cctttggctt tagggtcaga
     8581 taccggaggc tcggtgaggc aacc
//
LOCUS       DAOSVE010000074         8551 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_4328_length_8551_cov_69.208804, whole genome shotgun sequence.
ACCESSION   DAOSVE010000074 DAOSVE010000000
VERSION     DAOSVE010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 8551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8551
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4328_length_8551_cov_69.208804"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            286..426
                     /locus_tag="PK016_06630"
     CDS             286..426
                     /locus_tag="PK016_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02772.1"
                     /translation="MFIKVTKSGKYEYAQLVRSYRERGVVKQEVLLNLGRLDQIAHNS
                     SI"
     gene            complement(430..1275)
                     /locus_tag="PK016_06635"
     CDS             complement(430..1275)
                     /locus_tag="PK016_06635"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HXL02773.1"
                     /translation="MITAQTRLLGVIGHPVAHSLSPLFQNAALSYLGLPFVYLAFDIS
                     PSYLEKSLEAMRIFSIRGLNVTVPYKEEVIPFLDVLEEEAQIIGAVNTIVNEEGKLIG
                     YNTDGVGLYRSLERKGVSVEGKKALLLGAGGAAKAAVWALWKKGIASIFIINRTRERG
                     ESLAHWVRAKFSLEIEVGEWGKMEEWDSLWQEAGVIVNATSLGLKGEEISLPWEKLSP
                     SVWIIDVVYRQGLTPLVRKARRSGIQSFDGKEMLIQQGAESFSLFTGEKAPLEVMEEA
                     LNFRG"
     gene            complement(1272..1769)
                     /locus_tag="PK016_06640"
     CDS             complement(1272..1769)
                     /locus_tag="PK016_06640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HXL02774.1"
                     /translation="MSLKRKITPCLMAKSLPDVSLDFLEKKGIRGLIIDLDNTLVPWG
                     GFELPPRVEAWIKEAQSRGFKIFLVSNALEERVNHFRESLDIPGISQAQKPRRGAFRL
                     ALAQMGLEKNQVAVVGDQLFTDVWGGNRLGLFTVLVSPVNEKELFFTRLVRRIERRVL
                     KRLKA"
     gene            complement(1766..2740)
                     /gene="mltG"
                     /locus_tag="PK016_06645"
     CDS             complement(1766..2740)
                     /gene="mltG"
                     /locus_tag="PK016_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HXL02775.1"
                     /translation="MKFKFILCLSLSWILCLFLALAFYYEGEKGEGKIVVIESGLSGR
                     EISCVLEKEGIVPSSLFRLFLFLSHQDKLIAGNYFFYPGESVKEVVKKLTQGPDYEKV
                     TFPEGFTSEEMAKVLEEKGICSAGDYLDFINKPELFSASWLEGVISLEGFLFPDTYYF
                     SPFTSPQKVIETQLHHFEEVFLPHYQTSHSFLSLKEVVILASIVEKEAKWKEEKPVVA
                     SVFINRLKQGIKLQSCATVVYALYREKGLRVENVTEEDLKVVSPFNTYLVSGLPPQAI
                     CNPGLDSLLSVLNPPATDYLYFVLQDDGKHAFSRTYEEHLHHKEDNNP"
     gene            complement(2730..3161)
                     /gene="ruvX"
                     /locus_tag="PK016_06650"
     CDS             complement(2730..3161)
                     /gene="ruvX"
                     /locus_tag="PK016_06650"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HXL02776.1"
                     /translation="MDREVMKRVMAIDWGEKRIGVAFNQGTGFSFPCKVIEAEGEEGV
                     NKVLALAEELKAEVIVIGLPLHLDASFREEARKVEKFKKKLEQSFGGEVVLWDERLST
                     KEAEKRLIEADLSRKKRRKVVDKVSAALILETYLSHTGNEV"
     gene            complement(3143..5758)
                     /gene="alaS"
                     /locus_tag="PK016_06655"
     CDS             complement(3143..5758)
                     /gene="alaS"
                     /locus_tag="PK016_06655"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HXL02777.1"
                     /translation="MTSVEIRKAFLQFFEEKGHQILPSASLIPEDPTILLTIAGMVPF
                     KSIFEGRVSPPFRRAATVQKCVRISDLERVGYTSRHHTFFEMLGNFSFGDYFKKEACS
                     WAWEFLTQNLLLPPEKLWISVHRHDEEAFAVWRDIVGIPEEKIVFLGDEDNFWASGPA
                     GPCGYCSEIYYDTGVERGCGRPDCRPGCDCDRYLEVWNLVFMEFDRGSDGSLRELPHK
                     NIDTGMGLERITSIIQKVQTDFETDLFLPIIKEIEKMSGVSYQREDLRPYFRIIADHV
                     RAITFLIGDGVYPANEGRGYVLRRLIRRAHRYGQKLSLHRPFLPELAFVVIEIMDYYP
                     ELRDREKLIAQVVLQEERKFEETLQLGMGYLEDLIARFREKGERVIPGREIFYLYDTY
                     GFPPDIASEILKEEGLSFSQEEFEKEMERQKTRARQAYEEKAQEFKERKDKEEIFSGL
                     VSDFVGYEEEEAETTILALFSGEKRVQKVEKGEEVMVVLEKTPFYPERGGQEGDKGFI
                     VSSQGEIKVEKVNAPSSSLIIHRGVVEKGIIKEGEKVRAQIEKWRRRAMEIHHTVTHL
                     LHRALREVLGEQVKQAGSWVGEEGLRFDFTHFAPLSGEEIKAIESLVNEKIFEDLPVH
                     ITFSTLEEAQKRGVVALFEEKYEKTVRIVRVGNYSAELCGGTHLTHTSQALLFKIKSE
                     SGIGRGLRRIEGVAGPFAFSYLNQKEQILQDITKAINVEEDKLEGYIEKVVDENKELK
                     KVLEKEKKWRIEDKIQSRISSINNEKVALISYLEEEDGLDLDFLKEVVDHLRPLIQKG
                     VVVLGLKKEGKIQGVLAGVGLPSEVNLGKMIKEWSQKIGGGGGGRPNLAQFGGLPEEK
                     WEELISLVDDWIEKL"
     gene            complement(5715..6743)
                     /locus_tag="PK016_06660"
     CDS             complement(5715..6743)
                     /locus_tag="PK016_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExsB family protein"
                     /protein_id="HXL02778.1"
                     /translation="MTGQYSSSVWAQDAIQKLREVASSFEQVVVAFSGGIDSTVALFL
                     SLQALGKEKVISCTVDWDIYFPSLARENIDYWVKSLGVSHVYLPGRKVMEEIMKAGPS
                     CNRCTKEAKLGTIRRYFGDKVLIVGGANQSDSWGKRGVKLLNNTYSPLFELSKEEIIN
                     LASSLSLPLLRIGENRLREGCLLKHLLKPLASPYQAQAVVKSNEILLKILKERNIERD
                     IANVKIIGPLSRNVALVNVKPLPSLHLREEITAILSSIEEIDEVSWVDSPVTLIARAN
                     LGQYRNPSSLHWLEKGKLQPEFAFPLTIHWMPSSNRRLHTFQIVDFKKENTSYDQCRN
                     QESFSSVL"
     gene            complement(6740..>8551)
                     /locus_tag="PK016_06665"
     CDS             complement(6740..>8551)
                     /locus_tag="PK016_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HXL02779.1"
                     /translation="NISSKSEGLLPLREKMVGREGEVENQLEEGDETWVVVTHVDEQG
                     YVWLSREQARYQGAWIDIEESQSQNKTLTAKVKKRVKGGLIVDVGINAFLPASCVDVV
                     PKNLDEFIDKTISVKVIESDRKTKNVVVSRKVVLEEERKRQKKELFDSLEVGQVRKGI
                     VKNITNFGVFIDLGGVDGLLHVSEVSWKKTSENLEELFEKGEEVEVKVIAFDPEKEEI
                     SLSLKRLQPDPWENIDEKIKPGDIVKGKVVSVRDFGVFVEIEEGLEGLIHISDLSWGY
                     VKHPQEVVKVGSLVEARVLEVDKNKRRISLGIKQVLPDPWEGIEEKYPLGSTVRLRIS
                     SITPRGVWGEIEEGVEGRIPLEELSWKRVNRVSEIVRRNQVVEAKILGIDTRNRQILL
                     SIRQLRPNPWEEIGREWKVGDLVEAKVRRLMNFGAFVELVPDVEALLPVSELDWEPVK
                     HPNQVLKRGQTVSLKIIEFNPEEQRIVVSRKATLPDPWEEIKSKYPVGSVHLKKVVRL
                     VDFGAFIELEKGWDGLVHISEISNQRISSPSETLKEGEKVKVKVIKLDDRERKIGLSI
                     KQASQEEEKDYTSSNGKVTLGDMVGGKLTDFLNSLKK"
BASE COUNT         2453 a         2123 c         1359 g         2616 t
ORIGIN      
        1 ttataatcta atcatgtccc acaattgctt tctggacact atttatgatt aaaccataaa
       61 aacagcctac ggcgtagaca aaagacaaaa accactgagg agaaaagctt ccctcagtag
      121 aggtacactc ccttgccaac tcccaaaaca caaaagacaa agaccataga gaaagacctt
      181 gtctttctct atgcgtggcg accttgagtt tttgactttc gatataattt actttttaaa
      241 gtttttaaca ttttaatcag ttttgcggga tactataata cactcatgtt catcaaagtg
      301 actaaatcag gaaaatatga atatgctcag ctggttcgct cctatagaga aaggggagta
      361 gtaaaacaag aggtgctatt aaatttaggt agattagatc agatagccca caattccagt
      421 atctagcgtt tagcctctaa aatttagagc ttcctccata acctcaaggg gtgctttttc
      481 tccagtaaaa agagaaaaac tttctgcccc ctgctgaatc aacatttctt tcccatcaaa
      541 gctttgaatc ccactccttc ttgcttttct aactaaaggt gtcaaaccct gccgataaac
      601 cacatctatt atccagactg aaggagagag tttctcccag ggcagggata tttcttctcc
      661 ctttaatcct aaagaagtgg cattcactat cactcccgct tcttgccaga gagagtccca
      721 ctcttccatt tttccccact ctcccacttc tatctccaga gaaaactttg ctcttaccca
      781 gtgagcaagg ctctctcctc tttcccgggt tcggttaatt ataaaaatag aagctatccc
      841 ctttttccac agagcccaaa ctgctgcttt cgctgcacca cctgctccta aaagcaaagc
      901 ttttttacct tctacgctca ccccttttct ctccaggcta cgatacaggc ctaccccatc
      961 agtgttataa ccgattaatt ttccctcttc gttaactatg gtgttaactg ctcctataat
     1021 ctgagcctcc tcttctaata catccagaaa agggattact tcttctttat aaggaacagt
     1081 gacgtttaaa cctctgatag aaaaaattcg catagcctcc agcgacttct ctagataaga
     1141 aggcgaaata tcaaatgcca gatagacaaa aggaagacca agataagaga gggcagcatt
     1201 ttgaaaaaga ggagaaagag aatgtgcaac agggtggcca ataactccca gaagccgggt
     1261 ttgagcagta atcatgcctt tagccttttc agtactcgcc tttctatgcg cctcaccaaa
     1321 cgggtgaaaa atagctcttt ctcatttact ggtgaaacaa gaacagtaaa aagacctaaa
     1381 cgattccctc cccatacatc agtaaaaagc tggtcaccta ccactgctac ctggttcttt
     1441 tctaaaccca tctgggccaa agctaaacga aaagcacctc gacgaggttt ttgagcctga
     1501 gaaatccccg ggatatcaag actctctcga aaatgattca ctctctcttc cagagcatta
     1561 gagaccagga aaatcttaaa acctcggctt tgagcttctt ttatccaggc ttccactctc
     1621 ggagggagtt caaaacctcc ccagggtacc aaggtattat ccaggtcaat tatcaaaccc
     1681 cgaatacctt ttttctcaag aaaatctaaa gaaacatcag gaagactctt tgccataaga
     1741 caaggggtaa tttttctttt caagctcatg gattattatc ctctttatga tgtagatgtt
     1801 cttcgtaagt tcgagaaaaa gcatgctttc catcgtcttg caaaacaaaa tagagataat
     1861 cagttgctgg aggattaaga acggaaagca aagaatccag acccgggtta catatagcct
     1921 gaggaggaag accagaaacg agatatgtat taaaaggaga gacaactttt aaatcttctt
     1981 ccgtcacatt ttctactctc aatccctttt ctcgataaag agcataaacc acagtagcac
     2041 aagactgcag ttttataccc tgtttcaggc gattgataaa aacggaggct accacaggtt
     2101 tctcctcctt ccattttgct tctttttcca ctatagaagc taatattacc acttctttca
     2161 aagataaaaa agaatgcgag gtttgataat gaggaaggaa gacttcctca aaatgatgca
     2221 gctgagtctc tattactttt tggggagagg taaagggaga aaaataatac gtatcaggga
     2281 aaagaaaacc ttccaggctg ataactcctt ctaaccaact agctgaaaaa agctctggtt
     2341 tattgatgaa atccaggtaa tcgccagcag agcaaatacc cttttcttct aaaacttttg
     2401 ccatttcctc agaagtgaaa ccttctggaa aagtaacttt ttcataatcg gggccttgag
     2461 tcaacttttt aactacttcc tttactgact ctcccggata gaaaaaataa ttaccagcta
     2521 ttagcttatc ctgatgggat aaaaaaagaa aaagccggaa aagggaagaa ggaacaatgc
     2581 cttctttttc cagaacgcag gatatttctc gtcctgaaag accgctttca atcactacta
     2641 ttttcccttc tcctttttcc ccctcataat aaaaagccag cgctaaaaaa agacacaata
     2701 tccagctgag agaaagacag agaataaact taaacttcat ttccggtgtg agaaagataa
     2761 gtttccagaa ttaaagcagc agaaacttta tctaccacct ttctcctttt ttttctgctg
     2821 aggtctgcct ctatcaatct cttttctgct tccttagtgg acaatctctc atcccacaac
     2881 actacttctc ccccgaaaga ttgttccaat tttttcttaa atttctctac cttccgggct
     2941 tcttcccgaa aggaagcatc aaggtgaaga ggtaaaccta tcactatcac ctcagctttt
     3001 agctcttctg ctaaagctaa aactttattt accccttcct cgccttctgc ttctatcacc
     3061 ttacagggga aagaaaaacc agtcccctgg ttaaaagcta ctcctatcct tttttctccc
     3121 caatctatag ccattactct cttcataact tctctatcca atcatccaca agagaaatca
     3181 gctcttccca cttttcttct ggcaatcctc caaactgggc taagttaggt cttcctcccc
     3241 cgcctcctcc tattttttga ctccactcct ttatcatttt tcctagattt acctccgatg
     3301 gtaatcccac accagcaaga actccttgaa tttttccttc tttctttaac cctaaaacta
     3361 ctaccccttt ctggataaga ggccggagat gatctaccac ctctttcaga aaatcaaggt
     3421 ctaacccatc ttcttcctcc agataggaaa taagggcaac tttctcgtta ttgattgatg
     3481 aaatccgact ctgaatctta tcttctattc tccatttttt ttctttttcc aaaacttttt
     3541 tgagttcctt attctcatcc actacttttt ctatatatcc ttccagctta tcttcttcta
     3601 cattgatagc tttagtgata tcctgtaaga tttgttcttt ttgattaaga taagaaaagg
     3661 caaaaggccc agccactcct tcgattctcc tcaggcctct tccaatgccg gattcggatt
     3721 taattttaaa aagcaaagcc tgggaagtat gagtaaggtg agtccccccg cagagctctg
     3781 ccgaatagtt tcctactctc actattctca cagtcttttc atatttttcc tcaaaaagag
     3841 ccaccactcc ccttttttga gcttcctcta aagtggaaaa ggtaatatgc accggcaaat
     3901 cttcaaaaat cttttcatta accagagatt ctattgcttt tatctcctcc ccggataaag
     3961 gagcaaagtg ggtgaagtca aaccgcagcc cctcttctcc tacccaggaa ccggcctgtt
     4021 taacctgctc tcctaaaact tctcgcaaag cacggtgaag aagatgagta acggtatggt
     4081 gaatctccat cgccctcctc cgccactttt caatctgagc acgtactttt tctccctctt
     4141 ttatgattcc tttttctacc actccccggt ggataattaa agaggaagaa ggagcgttta
     4201 ctttctctac ttttatttct ccttgagaag aaacgatgaa acctttatct ccctcctgtc
     4261 ctcctctttc aggataaaaa ggagtttttt ctaaaactac cattacttct tctccttttt
     4321 ccaccttttg cactcttttt tccccagaaa aaagagccaa aattgtggtt tctgcttcct
     4381 cttcttcata acccacaaag tcactcacca agccagagaa aatttcttct ttatcttttc
     4441 tttccttaaa ttcctgtgcc ttttcttcat aagcctgacg agccctggtt ttttgtcttt
     4501 ccatttcttt ttcaaactct tcttgagaaa aagacaaacc ttcttctttt aatatttccg
     4561 aagctatatc aggagggaaa ccataagtgt cataaaggta gaaaatttct cttcccggaa
     4621 ttactctttc ccctttttcc cgaaaacgag caatcaaatc ttccagatat cccatcccca
     4681 gttgcaaagt ttcctcgaac tttctttctt cctggagcac tacctgggca atcaattttt
     4741 ctctatctct cagttctgga taatagtcca taatttctat cactacaaaa gccagctccg
     4801 gtaaaaaagg acgatgtaga gaaagcttct gtccgtagcg atgagcccgg cgaatcaaac
     4861 ggcgcaaaac ataacctcta ccttcgttag caggataaac tccatcccct atcaaaaaag
     4921 taatggctcg aacatggtca gcaataattc gaaaataggg gcgcaaatct tctctttgat
     4981 agctaactcc gctcatcttt tctatctctt taattatcgg taaaaaaagg tcggtttcaa
     5041 aatcagtctg aactttttgt attatcgagg taattctctc caaacccatt ccagtatcga
     5101 tgtttttatg gggtaattcc cggagagaac catcacttcc ccggtcaaac tccataaaca
     5161 ccaggttcca tacctctaaa tatcggtcac aatcacaacc cggacgacaa tctggccgtc
     5221 cgcatcccct ctccactcct gtatcataat aaatttcaga acaataacca caaggacctg
     5281 ctggtcctga agcccaaaaa ttatcttcat cccccagaaa cactatcttt tcctccggga
     5341 tacctactat atccctccag actgcaaatg cttcttcatc atggcggtga accgagatcc
     5401 ataacttctc gggaggaagc aatagatttt gagtgagaaa ttcccaggcc caggagcagg
     5461 cttctttttt gaagtaatca ccaaaagaaa aattgcccag catctcaaaa aaagtatgat
     5521 gacgagaggt atagcccact ctctctaagt cgcttattcg cacacacttc tgtaccgtag
     5581 ctgcccgacg aaaaggaggc gatactcggc cttcaaaaat ggacttaaaa ggcaccatac
     5641 cagcaatagt caaaagaata gtggggtctt cggggataag agaagcactg ggtaaaattt
     5701 ggtgtccttt ttcttcaaag aactgaagaa aagctttcct gatttcgaca ctggtcatag
     5761 cttgtattct cctttttaaa atccactatt tgaaaagtat gaagtcttcg attagaagag
     5821 ggcatccagt gaatagtaag ggggaaagca aattcaggct gcagttttcc tttctctaac
     5881 caatggaggg aagaaggatt acgatactgt cctagattag ctctggctat taaagtaaca
     5941 ggactatcca cccagctaac ctcatctatt tcctcgatag aagaaagaat agcggttatt
     6001 tcctccctca aatgtaaaga aggcagaggt ttaacgttaa ctaaagcaac attgcgacta
     6061 agaggaccaa ttattttcac attagctata tccctttcta tatttctctc ctttaatatt
     6121 ttaaggagaa tctcgttact ctttaccact gcctgagcct gataaggaga agctaaaggc
     6181 tttaaaagat gttttagtag acacccttct cgaagtctat tttcccctat ccttagcaaa
     6241 ggaagagaaa gagaagaagc cagatttata atttcttctt tactaagttc aaaaagagga
     6301 gaataagtgt tattaagtaa ctttactcct cttttccccc agctatcact ctggttagcg
     6361 cctcctacaa ttaaaacttt atctccgaaa taacgacgga tagttcctaa cttggcttct
     6421 ttagtgcaac gattgcagga aggaccggct ttcataattt cttccatcac ttttcgccca
     6481 gggagataaa catggcttac ccctaagctt ttcacccagt agtcaatatt ttcccgggct
     6541 aaagaaggaa aataaatatc ccagtccaca gtacaagata ttaccttttc ttttcccaag
     6601 gcttgaagag aaagaaaaag ggctacagta ctatctatcc ctccagaaaa agccactact
     6661 acttgttcaa aagaagaagc tacttctctt agtttctgga tagcatcctg agcccatact
     6721 gaagagctgt actgaccggt cattttttga gactgttcaa aaagtcagtt aatttgcctc
     6781 ccaccatatc tcctaaggtt actttaccat tggaagaagt ataatctttt tcttcctcct
     6841 gcgaagcttg tttaatactc aatccaatct ttctttcccg gtcatcaagc ttgatgacct
     6901 ttactttcac tttttcgcct tcttttaatg tttcagaggg agaagagatg cgctggttag
     6961 aaatttccga gatatgaact aaaccatccc accctttttc taactctata aaagcaccga
     7021 aatctaccaa tctaactacc tttttaagat gaacactacc tacaggatat ttactcttaa
     7081 tttcctccca ggggtcggga agagttgctt ttctgctaac cacaattctc tgttcttcag
     7141 gattaaactc tataattttg agagatacag tctgaccccg cttcagcact tgattaggat
     7201 gtttaactgg ctcccaatct aactccgaaa caggaagcag agcttctaca tctggaacca
     7261 gctctacaaa agcaccaaaa ttcatcaacc tccgtacttt tgcttctact agatctccta
     7321 ctttccattc tctgccaatc tcttcccagg gattaggtcg aagttgtcga atactcaaaa
     7381 gaatttgccg attccgggta tcaattccta aaatttttgc ttctactacc tggtttctcc
     7441 ttactatttc gctgacccgg ttaactctct tccaggataa ctcttcaagc ggtattcgtc
     7501 cctctactcc ctcttcaatc tccccccata ctccccgggg ggtaattgaa gatattctca
     7561 atcggacggt gctacccaga ggatattttt cctctatacc ttcccagggg tcgggcaaaa
     7621 cctgtttaat acccagagag attcttctct tgtttttatc tacttccagt acccgagctt
     7681 ctactaggct acctactttg accacttctt gaggatgctt cacataaccc caggaaaggt
     7741 cggaaatatg aattaatcct tccagacctt cttctatttc tacaaatact ccaaagtctc
     7801 gaacagaaac tactttacct ttaactatat caccaggttt tattttttca tctatatttt
     7861 cccaggggtc aggttgcagt ctcttaagac ttagagatat ttcttccttc tccgggtcaa
     7921 aagcaataac ctttacttct acttcttctc ccttttcgaa gagttcttct aaattttcag
     7981 aggttttctt ccaggaaact tctgaaacat gtaagaggcc atctactcct cctaaatcta
     8041 taaaaactcc aaaattggtg atatttttta ctattccctt tcttacttgc cctacttcca
     8101 gggagtcaaa caattccttc ttctggcgct ttctttcttc ctccagaact acctttctcg
     8161 acaccactac attctttgtc ttcctgtcag attcgattac tttaactgaa atagttttat
     8221 ctataaactc atccagattc ttggggacaa catcgacaca agaagcaggt aaaaaggcgt
     8281 tgattcctac atctacaata agtccacctt taactctctt tttgacttta gcagttaaag
     8341 ttttattttg actttgactc tcttctatgt ctatccaggc tccctggtaa cgagcctgtt
     8401 ctctggagag ccatacatag ccctgttcat cgacatgggt aactactacc cacgtttcgt
     8461 caccttcttc taactgattc tctacttctc cttctctgcc caccattttt tctcgcaaag
     8521 gcaataaacc ttccgattta gagcttatgt t
//
LOCUS       DAOSVE010000075         8507 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_4349_length_8507_cov_78.258519, whole genome shotgun sequence.
ACCESSION   DAOSVE010000075 DAOSVE010000000
VERSION     DAOSVE010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 8507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8507
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4349_length_8507_cov_78.258519"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PK016_06670"
     CDS             complement(<1..290)
                     /locus_tag="PK016_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02780.1"
                     /translation="MSNYLTLVRAVYSGISQRQVAKSHQVSRNTIALVLQHAQKRGWL
                     TLASLASISEEEFCCDFHLAKANEQEQFIKFKRQIAKIPLLIIDEWLLFPIT"
     gene            complement(669..1451)
                     /gene="trpA"
                     /locus_tag="PK016_06675"
     CDS             complement(669..1451)
                     /gene="trpA"
                     /locus_tag="PK016_06675"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HXL02781.1"
                     /translation="MTDILKGKLEGRRQRGKLFLPYLTFGYPDIPTFLDLMHICEEEG
                     VDALEVGIPHSDPIADGPVIQTTSQRALEKGVTPQLVAQTLKEEETTLPLIAMTYGNI
                     VYQYGFSRFARDFQNAGFSGLIIADFPREARNFEKDIEGYLSRILLASVTSPPERIKQ
                     IAKESEGFVYLVSGKGTTGQVEIEKEHLREVVEVIKGVSSSLVLIGFGVDSPEKAKGL
                     AQIADGVIVGSALLQVILQEEKHPQPAFRRLLSSYRQALDEV"
     gene            complement(1444..2628)
                     /gene="trpB"
                     /locus_tag="PK016_06680"
     CDS             complement(1444..2628)
                     /gene="trpB"
                     /locus_tag="PK016_06680"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HXL02782.1"
                     /translation="MENLGFFGEFGGRFVPEILVHPLEELEEAYQYYRKDKQFQEELN
                     YYLENYAGRPTPLYFAQSLSKSWGGARVYLKREDLNHTGSHKINNTLGQALLAKKMGK
                     KRIIAETGAGQHGVATATVAALLDLECQVYMGAKDIERQKLNAFRMEILGAEVIPVYS
                     GSQTLKDAINEAMRDWVRNLDTTHYVIGSVVGPHPYPQMVRDFQSIIGRETKQQIMEK
                     EGRLPDYALACVGGGSNAMGMFYEFVPEKVALIGVEAGGMGIESGKHAASIGGGRKGI
                     LHGSLSYILQDEFGQIQETHSVSAGLDYPGVGPEHSYLFSSGRAQYVSVNDEEAVGAF
                     LELSKKEGIIPALESAHAVAYASKIAPKLPGKVMVICLSGRGDKDVEEVLRYLEEEKK
                     ND"
     gene            complement(2630..3235)
                     /locus_tag="PK016_06685"
     CDS             complement(2630..3235)
                     /locus_tag="PK016_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HXL02783.1"
                     /translation="MPRVKVCGIKEKNDVLLLSEFPLWALGFILVEDSPRFILPEKAK
                     EIVSSLPSRIIKVGVFQNQSAEEVKRLQVFCGFDLIQLHGREDPAFCENLGRGIIKAF
                     SVGEEFDTREVEEYLPVVDFLLFDGKRGGSGMTFPWGKIKGVDFPRPIIIGGGLREEN
                     ILQCISLLHPFALDLNSGVEIYPGKKDPEKVRKILEKAGGK"
     gene            complement(3222..4022)
                     /locus_tag="PK016_06690"
     CDS             complement(3222..4022)
                     /locus_tag="PK016_06690"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HXL02784.1"
                     /translation="MMEGFLEKIVGGKKRRLLEGKQYSPREVVEIFKRREGRRNPLLP
                     SLKYLSGPNIIAEIKRASPSRGKLIEGSFLPYLEEYEAGEAVAISVVTEEDYFKGSLE
                     MFQEVRKFTSLPLLRKDFVIEETQIYESAQWGADALLLIARILSRERLQRFVYLCTLL
                     NLVPWVEVHDEKDVEKALEAGAPLIGINNRNLATFQVALEISERLRREVPPSIPVVAE
                     SGIKNRADIEKLMDKGINNFLIGETLLASLHPAEKLKELQRGAKVAQS"
     gene            complement(4019..5053)
                     /gene="trpD"
                     /locus_tag="PK016_06695"
     CDS             complement(4019..5053)
                     /gene="trpD"
                     /locus_tag="PK016_06695"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HXL02785.1"
                     /translation="MLKTARDFLEYLIEGKRLSRKDSYSLMRNLMEGEFTSSQMGALL
                     SSLRMRKEDGEEITGFAEAMREKAVNFSISSLSVADNCGTGGDGQGTFNISTASAFVA
                     LSSGLKIVKHGNRSVSSRCGSADLLENLGIKVDMEREKMQILFEETGFAFLYAPLYHP
                     ATRAVQGVRKELGIRTVFNLLGPLTNPCPMDYKLVGVYSPELIEPIASTLVSLGVKRG
                     LVVWGEPGVDEISTIGESKLALVEGDKITFSSFHPEEAGFPTRDIEELRGGTPPENAQ
                     IFMNILQGEEKGARREAIVLNSAFLLWIGEKARDLEEAIEKVEEAIDSGRVLQTLKKV
                     VRISRALEGK"
     gene            complement(5047..5623)
                     /locus_tag="PK016_06700"
                     /pseudo
     CDS             complement(5047..5623)
                     /locus_tag="PK016_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514407.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
     gene            complement(5623..7029)
                     /locus_tag="PK016_06705"
     CDS             complement(5623..7029)
                     /locus_tag="PK016_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HXL02786.1"
                     /translation="MEIKPEKGKFKKLSAEGYDFIPLYTEQLADRLTPVSLVDKFSEE
                     TPLVLLESAERGEKWGRYSFLIPGGEEVKVEGFVDIISLLEEKFPSYSVYPEPPLPFL
                     GGAVGFLSYDAVKTWEKTIPRRKINYPLSYWIKSNRFLYFDHLKHLLGIVYIARGDEE
                     MDYDEGKKWIEETYNRLLQPSYSPSSSFEVRGEVEHSLTEGEFREKVEKVRDFIREGH
                     TSQVVISQRFSQSFSGDPFVPYRFLRSLNPSPYLFYLKTDQFQIAGSSPEMLIKIEKG
                     KIFTKPIAGTRKRLSSTPEEEIIEDLLHDEKEIAEHVMLLDLGRNDLGRVCERGTVQV
                     EKFMDVERYSHVFHIVSTVSGNLAPRISPWRALQASFPAGTVSGAPKVRAMEIIEELE
                     PLPRGPYAGALGYVSLNGNLDTCIVIRSIFFREGQAEVQAGAGIVYDSLPQREYEEIR
                     SKAEALLLALNLAGRRNS"
     misc_binding    complement(7058..7305)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7449..8453
                     /locus_tag="PK016_06710"
     CDS             7449..8453
                     /locus_tag="PK016_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXL02787.1"
                     /translation="MRKKDNSLYDLALRFYEKGRRAIVPLFGYSGLQFTGTTIKQNQF
                     NHLVQFHSLSRLYDRFRPDAMFFLMDLSVEASALGLPVRFPLEETPSVEAHLIKSKND
                     LDKFRKIDILGDGRVIVYLETLRHMRAAFPCPVAAYVIGPLTLSGLLMGANELAIKSI
                     LEPDFFEEVLEFSHQVIMRYAQALRENGAESIMVLDPTAVIFGPQQFRQTVSRFYREM
                     VGILDDVEITLHVCGNTFHLLEEMVDSGVAGLSLDSLVNMKEASQVVGEGILLMGNIS
                     PVKMLFGSPEEVYGETWNLLESMRDFPLFILSTGCDLPQDVPLGNIDAFFQAGRDWKK
                     "
BASE COUNT         2224 a         1988 c         1696 g         2599 t
ORIGIN      
        1 tctgtgatag gaaagagaag ccattcatcg ataataagta aaggaatctt tgctatctgg
       61 cgtttgaact taataaactg ctcttgttca ttggcctttg ccagatgaaa atcacagcaa
      121 aattcttctt cactgatgga cgccaatgat gcaagcgtta gccatcctct cttttgggca
      181 tgctgtagaa caagggcaat cgtgttacga gatacctggt ggcttttagc tacctgtcgt
      241 tgacttatac cactgtaaac agcacgcact aaggttaagt aatttgacat atcaaacctc
      301 ctgtaaaaag atttaccact ttcgtggcat gagataatcc tacaggattt ggtttgttaa
      361 ggtggtacta aatgggcgcg ctactggcgc ttttactccg cgcgcttggt tctaaacagc
      421 cgtgtttcgc accccgtctg tttgcttatg tgacaatcta tttcccctat ttatgaaatc
      481 ttctattagg tattcactga ggaagacgct ttttcttcct caacgctgga gtgcatttat
      541 ccagcgtaat tagttttagt ttttttcatc tattttgctt ttttatgttc tttgtctttt
      601 cccggggatt ataaaggggc agagcccctt tatagtttag attcctggta gagctattta
      661 taattttatt atacctcatc cagagcctgg cgataagaag aaaggaggcg gcggaatgca
      721 ggttgaggat gtttttcttc ctgaagaatt acctgcagga gcgccgatcc cactatcact
      781 ccgtcggcaa tctgggccag tcctttagct ttctctggag agtccactcc aaagccgatg
      841 agaaccaaag aagaagagac accttttata acctctacta cttctcggag atgttctttt
      901 tctatctcca cctgcccggt cgtgccttta ccggaaacca gatagacaaa accttcactc
      961 tctttagcaa tctgctttat cctctccggg ggagaagtga ccgaagcaag caatattcgg
     1021 gataaatatc cctctatatc tttttcaaaa tttcttgctt cccgggggaa atcggcgatg
     1081 attaaacctg aaaatccggc attttggaaa tctcgagcaa aacgagaaaa accatattga
     1141 taaacgatgt taccgtaagt catagctatc aaaggaagag tggtttcttc ttctttcaaa
     1201 gtttgcgcaa ccagctgagg tgtaactcct ttttccagag ctctctggga agtagtttga
     1261 attacagggc catcagctat ggggtcagag tgaggaatgc cgacttccag cgcatctacc
     1321 ccttcttctt cgcagatatg catcaaatcc agaaaagtgg gaatatcggg ataaccaaaa
     1381 gtgaggtagg gaagaaaaag ttttcctctt tgtcttctcc cttctaactt gccttttaat
     1441 atgtcagtca tttttctttt cctcctctaa atagcgtaaa acttcttcta cgtccttgtc
     1501 tcctcgtccg gagaggcata tcaccataac tttccccggg agttttgggg ctattttaga
     1561 agcataagct acggcatggg cactctctaa agccggtatg attccttctt ttttagaaag
     1621 ttctaaaaat gctcctactg cttcttcatc gttaactgag acatactgag cccggccgga
     1681 ggagaaaaga taactgtgtt ctggccccac cccggggtag tcaaggccgg cagatacgga
     1741 atgagtctcc tgaatttgtc cgaactcatc ctgtaagatg tagctcaggc ttccgtggag
     1801 aatccctttt cttcctcctc caatactggc agcatgtttt ccgctttcta ttcccattcc
     1861 tcctgcttct actcctataa gagctacctt ttccggaacg aattcgtaaa acatccccat
     1921 agcattactt cctcctccca cgcaggctaa agcgtagtca ggtagtctcc cttctttttc
     1981 cattatctgc tgtttagttt ctctaccgat tatggactga aaatctcgta ccatctgagg
     2041 ataaggatga ggtcccacaa cagaaccgat aacataatga gtggtatcta aattcctcac
     2101 ccagtctctc atagcttcgt taatagcatc tttcaaagtc tggcttccac tgtagaccgg
     2161 aatcacttct gctcctaata tctccatgcg aaaagcattg agtttttgtc tttctatgtc
     2221 tttggctccc atatatacct ggcactctaa atctaaaaga gcggcaacag tggcggtagc
     2281 tactccatgc tgtccggctc cggtttcggc aataattctc tttttaccca tttttttagc
     2341 caatagcgcc tggcccagag tattatttat tttatgagag ccggtatggt ttaagtcttc
     2401 tctttttaga taaacccgtg ctcctcccca ggatttgctg agggattgag caaaataaag
     2461 cggggtgggt cttccagcat agttttccag gtaataattt agctcttcct ggaattgttt
     2521 atcctttcgg taatattggt aagcctcttc taactcttct aaggggtgaa ctaaaatttc
     2581 tggaacaaac ctccccccga attctccaaa aaatcccaga ttttccattt cattttcctc
     2641 ctgccttctc taaaattttc ctcacttttt ccgggtcttt tttcccgggg taaatttcca
     2701 ccccgctatt taaatccaga gcgaagggat gcaagagaga aatacactgc aggatatttt
     2761 cttctctcaa gcctcctccg attataatcg gtctgggaaa atcaactcct tttatctttc
     2821 cccaggggaa agtcatcccg cttcctcctc ttttaccatc aaaaagcagg aaatctacca
     2881 ccggaagata ttcttctact tcacgagtat caaattcttc tcccacagaa aaggctttga
     2941 ttattcctct ccctaagttc tcgcaaaaag cagggtcttc tctaccatgt aattgtatta
     3001 agtcaaaacc acaaaagacc tgcagccttt ttacctcctc tgcactctga ttttgaaaaa
     3061 ctcccacctt tattatccgg gaaggtagag agctaactat ctcttttgct ttctctggaa
     3121 ggataaagcg ggggctatcc tccaccagaa taaatcccag agcccagagc ggaaactcac
     3181 tcaaaagcag aacatcattt ttttctttaa tgccacaaac tttaactctg ggcaacttta
     3241 gctcctctct ggagttcttt cagtttctct gccggatgca gagaggcaag cagcgtctcc
     3301 cctatcaaga aattgtttat tcctttatcc atcagttttt cgatatctgc tctattttta
     3361 attccgcttt ctgctactac cggaattgag ggtggaactt ctcgacgaag tcgctctgaa
     3421 atttcaagag caacttgaaa agtggccaga ttacgattgt tgataccaat taaaggagcg
     3481 ccagcttcca gcgctttttc tacatccttc tcatcatgaa cttccaccca ggggacgagg
     3541 ttcaaaagag tacagagata aacaaagcgc tgcaagcgtt cccgggatag aatacgagca
     3601 atgagaagaa gagcatctgc cccccattga gctgactcat aaatctgggt ttcctctatc
     3661 acaaaatcct tacggagaag agggagagag gtaaattttc tcacttcctg aaacatctcc
     3721 aggcttcctt taaagtaatc ctcttccgta accacagaaa ttgccaccgc ttctcctgct
     3781 tcgtattctt ctaaataagg aaggaaactt ccttctatca atttccctcg agaaggagaa
     3841 gctctcttaa tctcagctat gatgttaggt ccggacagat atttaagaga aggaagaaga
     3901 ggatttctcc tcccctctct tctcttaaat atctccacta cttcccgggg agaatattgt
     3961 tttccctcta gaagcctcct tttctttcct cctactattt tttccagaaa cccctccatc
     4021 attttccctc cagagctcta cttatcctca ccactttttt caacgtttgt aacactctcc
     4081 ctgaatcaat agcttcttct accttttcaa tcgcttcttc taagtctcga gccttttctc
     4141 ctatccagag caaaaacgca ctattcaaaa ctattgcttc tcttcgtgct cctttttctt
     4201 ctccctggag gatgttcata aaaatttgag cattctcggg gggcgttcct ccccgaagtt
     4261 cctctatgtc tcttgttgga aaacctgctt cttcggggtg aaaggaagaa aaggttattt
     4321 tatctccttc taccaaagct aatttacttt ctccaattgt acttatctca tctactcctg
     4381 gctctcccca taccaccaat cctcttttta ctcctaaaga aactaaagtt gaggcgatag
     4441 gttcaataag ctcgggagaa taaactccca ccagcttata atccatggga caaggattgg
     4501 taagaggtcc taagagattg aagacagttc tgattcctaa ttcttttctt actccctgta
     4561 ccgctcgtgt ggcaggatga tagagaggtg cgtagagaaa ggcgaaacct gtttcttcaa
     4621 aaaggatctg cattttctct ctttccatat ctacttttat tcccaggttc tccagtaaat
     4681 ctgcgctgcc acagcggctg gaaactgaac gattaccatg ttttactatt tttagtccgg
     4741 aggataaggc cacaaaggca gaagcggtgg aaatgttgaa agtcccctgg ccatctcctc
     4801 cagttccaca gttatcggcc acagaaagag aagaaatcga gaaatttact gctttttctc
     4861 tcattgcctc ggcaaacccg gtaatttctt ctccatcttc tttgcgcatc cgcagggaag
     4921 acaggagtgc tcccatctgg gaagaagtaa actctccttc catcaagttt ctcatcaggg
     4981 aataagagtc cttcctgctt aacctttttc cttctatcag atattctaaa aaatctcgag
     5041 ctgtttttaa catgagagtc ccacctccag aaaattcttt agaatggttt taccctgagg
     5101 ggttaaaact gattcggggt gaaattgcac cccgaaaaga gggcgataaa tatgttgcaa
     5161 agccattatt tcttcacttt ctgtttgggc aatgactttc agggaaggtg gtaaactctc
     5221 tcgctcaaca atgagggagt gatatctacc tacccagatc tggggaggca atcctcgaaa
     5281 aagaggagag gggaaaaggc aaactaaaga ctttttcccg tggcaaggtt ggggagcttt
     5341 tactattttc cccccttcaa cataggcaat gcactgatgg ccaaagacac acccctaaag
     5401 tagggataga ggggaaatga gtaattatct cattggtgat tcctgcctcc cggggctctt
     5461 tgggaccggg agagataaca attttggttg gtttcatttt ttccacctga gagagggaaa
     5521 cctcatcatt gcggaatact ttgatttcct ccgcagttag ctctcctaaa tactgcacta
     5581 agttgtaggt aaaagaatcg taattatcaa tcaagagaat cattaactgt tcctccttcc
     5641 agctaaattt aaagctaaga gtaaagcttc ggctttactc cgtatttcct catattctct
     5701 ctgagggaga gaatcataaa caattcctgc cccggcctgg acttctgcct ggccttcccg
     5761 gaaaaagatg ctgcggatta cgatgcaggt atccagattt ccgtttaagc tcacataccc
     5821 taacgctcca gcgtaaggcc ctcgagggag aggctccagc tcttcgatta tttccatggc
     5881 tctcacttta ggtgctccgc taactgttcc tgcaggaaaa gatgcctgta gtgctctcca
     5941 gggagagata cggggagcta aattaccgga aacagtggag acgatgtgaa aaacatggga
     6001 atagcgctct acatccataa atttttccac ctgtactgtt cctctttcgc acactctccc
     6061 taaatcattt cttcccaggt ctaagagcat cacgtgttcg gcaatttctt tttcgtcatg
     6121 gagtaaatct tctattattt cttcttcagg agtggaagat agtctttttc tcgttccagc
     6181 gatgggttta gtaaaaattt tccccttctc tatttttatg agcatctccg gagaagagcc
     6241 ggctatctga aactggtcgg tcttcaaata aaaaagataa ggagaaggat tcaaggaacg
     6301 caaaaaccgg tagggaacaa aagggtcacc agaaaaagac tgagaaaagc gctgagaaat
     6361 gaccacttga gaagtatgcc cctcccggat aaagtctctc actttttcta ctttctctct
     6421 aaattctccc tcagtgaggg aatgctccac ctctccccga acttcaaaag aagaggaggg
     6481 agaatatgag ggctggagta atctattata agtttcttct atccattttt ttccctcatc
     6541 ataatccatt tcttcatccc cccgagcaat atatactatc cctaagagat gtttcaggtg
     6601 gtcaaaatag agaaaacggt tgctctttat ccagtaagat agaggatagt ttatcttccg
     6661 ccgaggaatg gttttttccc aggttttcac tgcatcatag ctcagaaaac ccactgctcc
     6721 tcctaaaaag ggaagaggag gttcgggata aacagaataa gaggggaatt tttcttccag
     6781 aagcgaaata atatcgacaa aaccctctac ttttacttcc tccccacctg ggattaaaaa
     6841 agaatagcgc ccccattttt ctcccctttc ggcactttcc agtaaaacca gaggagtttc
     6901 ttcactaaat ttatccacta aggataccgg agtaagccgg tcggccagct gctcggtata
     6961 gagaggaatg aaatcataac cctctgccga caatttctta aatttcccct tttctggttt
     7021 tatctccata ttttcctccc atataaaaaa gccctcgtca agagacgagg gcttaaactc
     7081 gcggtaccac tctaattaac ggcgcaaacc gttcactcgt tcggtacaaa aactactata
     7141 cctactttct ttagcgggaa agaaccgtct caacctactc gagtattctc tttcgatgag
     7201 ctgctccgag gcccattcac ttttttctcc agcaccaggc tctcacctgc cctggctctc
     7261 tgtggttgaa ggaaaaaagc tactctccct catcattgct tttcttattg gcgcttatta
     7321 aagcacaact ttttctcttc gtcaaggggg gtaggctgac attgggaaaa tcctgtattt
     7381 tttctttcac tttcttacct tcaggtgcta ttgtgctatt atgttgttat aaattgtagg
     7441 tggtggaaat gagaaagaaa gataattctt tgtacgattt agccctccgt ttttatgaga
     7501 agggaaggcg tgctattgtc cctctttttg ggtattctgg tcttcagttt actggaacta
     7561 ctattaagca gaatcaattt aaccacctgg tacaatttca ttctctgagc cgtctttatg
     7621 accgtttccg tcctgatgcc atgtttttcc tcatggattt gtcggtagaa gccagtgctt
     7681 tggggttgcc agttcgtttt cctttagagg aaactccttc agtggaagct catctaataa
     7741 agagtaaaaa tgacctggat aaattccgta aaattgatat tctgggtgat ggaagggtta
     7801 tcgtttattt ggaaacactg agacatatgc gagctgcttt tccttgcccg gtggcagctt
     7861 acgttatagg tcctttaact ttatcgggtt tgttaatggg agctaatgag ctggcaatta
     7921 aaagtattct ggagccagat ttttttgaag aggttttaga attttctcat caggtaatta
     7981 tgagatatgc tcaagctttg cgagaaaatg gagcagagtc tattatggtt ctagacccta
     8041 ctgcagtaat ttttggtcca cagcaatttc gtcaaactgt ttctcgtttt tatagggaga
     8101 tggtaggaat tctggacgat gtggagatta ctcttcatgt gtgtggcaat actttccatc
     8161 tcttggagga gatggtagac tctggagtag caggtttgag cctggatagt ctggtaaata
     8221 tgaaagaagc atcccaggta gtaggagaag gcattctact tatgggaaat ataagcccgg
     8281 ttaaaatgct gtttggctcc ccggaagaag tttatggtga aacctggaat cttttagaaa
     8341 gcatgaggga tttcccttta tttattttga gtactggttg tgacctgcct caggatgtgc
     8401 ctttagggaa tatcgatgcc ttttttcagg cagggaggga ctggaaaaaa taggttagcc
     8461 tgcagtaaag ctgtgagggg gcaatgcccc cactcataac tcccaga
//
LOCUS       DAOSVE010000076         8399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_4398_length_8399_cov_68.373562, whole genome shotgun sequence.
ACCESSION   DAOSVE010000076 DAOSVE010000000
VERSION     DAOSVE010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 8399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8399
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4398_length_8399_cov_68.373562"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..461)
                     /gene="cas2"
                     /locus_tag="PK016_06715"
     CDS             complement(198..461)
                     /gene="cas2"
                     /locus_tag="PK016_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003871087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HXL02788.1"
                     /translation="MFIIMVYDVNQSRVTKVLNIARKYLNWVQNSVLEGELTDAKYEK
                     LKAEVTKKIKLEEDSVIFYILRTTRYSEREIIGVEKGGHQTII"
     gene            complement(462..1457)
                     /gene="cas1b"
                     /locus_tag="PK016_06720"
     CDS             complement(462..1457)
                     /gene="cas1b"
                     /locus_tag="PK016_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1b"
                     /protein_id="HXL02789.1"
                     /translation="MSESIYIFSSGKLERKHNTIYFEDSEGGRKYVPIENTREIHIFG
                     EVSLNKEILEYLSQNEILLHFYNYYGYYSGSFYPREHYNSGYLILKQAEHYLDEAKRL
                     TLAKSFVQGALQNMGKVLSYYNNRGVELSPLIEKINSFSQSAEEQETVESLMAIEGNA
                     RQIYYSSFDSIVQDEDFNLESRTKRPPENRMNALISFGNSLLYTIVLSEIYKTHLDPR
                     IGYLHTTNFRRFTLNLDVAEIFKPILVDRVIFYLLNKKMIGSENFDERVGGIYLDESG
                     RRAFVERFEERLKTTINHSKIGRQVSYRRLIRLELYKLEKHFMGEENYAPFGAEW"
     gene            complement(1454..1954)
                     /gene="cas4"
                     /locus_tag="PK016_06725"
     CDS             complement(1454..1954)
                     /gene="cas4"
                     /locus_tag="PK016_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012996176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HXL02790.1"
                     /translation="MVDGHVSGTLVQSYSICQRQLWLMAHQITPDQDNSYLEIGRFLD
                     TESYDRDKKKIHFENVVLDLVQTKERNLVVGEIKKSSRAEESARLQLLFYLYRLRESG
                     ITSEGVLLFPKERKRIKVDLTPELEEKMKNVIEDIKRIIALPSSPPPRKIKFCTNCAY
                     REFCWA"
     gene            complement(1948..4311)
                     /gene="cas3"
                     /locus_tag="PK016_06730"
     CDS             complement(1948..4311)
                     /gene="cas3"
                     /locus_tag="PK016_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HXL02791.1"
                     /translation="MRGVEGFSFSNLYSHPGKYLEEHLIRVAKIALTNWEAAPINTLG
                     EFSKSDLTRLVKICGLAHDLGKSTNAFQKYLLSENPGKSKETRHSLLSAIATFFLVED
                     EFREATHLSADQKKLLAFVGFLAVKRHHGNLEDVLTESFLDTKEEELLLKQIEDIDAD
                     RLSILVENLKISGLPSGITRDSWSGWVEAFPSEVIKMRRTLRQLSKENNLSWYFLTNL
                     VFSLLIDADKSEVTVGELSRKEVSIPPSIVSEYKSTLKRGETYLNQLREEAYQEVINQ
                     EIDLATRFFSLDLPTGLGKTFTSFAFALKLREKIKEEKGYTPRIIYSLPFLSIIEQNA
                     DQIEKILKNSGLAVDSSIILKHHHLSDIYYQEDEKEFEPDQAKILIEGWNAEIIVTTF
                     VQFFHSLISHRNSTLRKLHRLAGSIIILDEVQSIPFKYWLLLREVFKHLTSSFNAYII
                     LVTATQPLILERGQMFPLVDPQKYFPQVNRITIRSDIEQSMTIEELVNYLTIKEDQSY
                     LFIMNTINAATTLYQLMREKVKDKIVYLSTHVVPWERLKRIKALQEKKFRLAVTTQLV
                     EAGVDIDFDVVYRDIAPLDSLIQSAGRCNRNWSKEKGLVNIVRLQDNKRAYSSYIYDS
                     LLLRETEKVLLKNNVLYEQDLFEMVENYFHLLQNKKSSDESRKLLEAVYRLKYTSNDQ
                     QVGIADFQLIEEDYPKLDVFLEINEEAQEVWNAFLEIKEIKDLWQRRLEFDKIKGDFY
                     QFVVSVPIKVENLPPEVSGFRYVNNDSLSDFYDSETGFKLKGGSAIW"
     gene            complement(4280..4999)
                     /gene="cas5b"
                     /locus_tag="PK016_06735"
     CDS             complement(4280..4999)
                     /gene="cas5b"
                     /locus_tag="PK016_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5b"
                     /protein_id="HXL02792.1"
                     /translation="MKVLVFDIYGDLAHFRKFYTTSSPLTFPFPPPSTIKGMLGAIIG
                     VDKKDYLRVFSGDHCKIGLQILRPVKKIRLGLNHINTKQNYWIPTKRGSHGARTQIRT
                     EFVKDPGYRLYVSHQDQAIFEDLVRKVKNHEAVFTPALGLSELLADFQYQKVLEVEER
                     RKEGETSLVTVIPLSYLEGLRINFEEGKKYFKERIPLRMNEERVVEEYQEVIFESEGK
                     PIRVETQKSWEGEGSGGLLFF"
     gene            complement(5014..5922)
                     /gene="cas7b"
                     /locus_tag="PK016_06740"
     CDS             complement(5014..5922)
                     /gene="cas7b"
                     /locus_tag="PK016_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas7/Csh2"
                     /protein_id="HXL02793.1"
                     /translation="MDVIQNRSEILFLYDVTNANPNGDPVDENKPRIDEETGINLVSD
                     VRLKRTIRDYLYDYLGKEVFVREIRDDNGNLKTKEDRLKDFSSPQDLIEKCIDIRLFG
                     ATTAVKEQTMALTGPVQFKYGRSLHRVDLTYVKGTTVMPSQSDKKQGTFTERYILPYS
                     LIAFWGVVNEKAAQAQNLPLTCEDVDLMMEAIWNGTKNLISGSKFGQTPRFLLQIIYK
                     GGNFYIGELDKRIAIQTEKEEEKIRDISELQVEISPLIKALQENQDKIEKLRIKTDDR
                     IVLAKDGEPVRVESAFSGLPTASFPF"
     gene            complement(5924..7708)
                     /locus_tag="PK016_06745"
     CDS             complement(5924..7708)
                     /locus_tag="PK016_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02556 family CRISPR-associated protein"
                     /protein_id="HXL02794.1"
                     /translation="MIEAIKEIGEIIIQRERKDLMEIMVEDPNINGKYTQVVTIILEK
                     RGNQVSFEEVDREEYDSSKKMRYLYRSGAGNGPDFSPTAKLTEPQKTFTNKILGWANR
                     VLQKNADLSPEEKELLHSLKRELEAHRDRIIGEIQKIREEIPRKEGIFITLKLRDENE
                     EYYPGDIPLFREIFTLGVQGGNKKRGGQFATCSICNEKKDVILGDGVYSFFTIDKPGF
                     IAGGFREEVAWKNFPICLDCKQALEEGKKYLEANLTFNFAGLRYQLIPKFIMGKENVA
                     QDVLDLFTDSPKLLSLKKESQNRFLGDEQEILLLLSQAQDNLTLNFLFLLKQQSAERI
                     LAFIEDVFPSHLKEIFEVKDEIDHAFQQNFTFRTIRNFFSKSDPAKRDNDLDGYFLDL
                     VDRVFKGRPVDYDFILHFLMNKIRSSFVNDQYFGPTTIEGVMVVNFLERMKLIKMEVM
                     AMDERLFDPLFKKYGATFETPLKRGLFLLGALTQLLLRVQYNRRESTPPFLKNLKGLR
                     MDERDFLGLLPRVENKLQEYDSFDQGKQKLAREAAHYLLEAGNNWKMSTDEMNFYFVA
                     GMNLLSEVTPFIYSEKSEKEKILVEEDV"
     gene            8124..>8399
                     /locus_tag="PK016_06750"
     CDS             8124..>8399
                     /locus_tag="PK016_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HXL02795.1"
                     /translation="MKRSLLILAVLGICLGSLLAVCSVEAQAVPPPKPTLKFNSQGTF
                     KIVMFSDVQDQFPINPQTIEAMNNILDFERPEFVILVGDNCTGAIGSA"
BASE COUNT         2383 a         1786 c         1523 g         2707 t
ORIGIN      
        1 ttcaatccct tataggtagg ctaaaaaccc gttcgagggg cttaaaataa ggatttctta
       61 tttcctattc acttttcaag gtgcacacct gaaattatta aaaactactg tcatcctttt
      121 gtcaagcgga gactaaaata tcttttcttt tctgtcgatc cccgggggtt tttgcactat
      181 tggacatcga cgaaaaatta gatgatcgtc tggtggccac ctttttctac acctattatt
      241 tctcgctcag aataacgagt ggtgcgtaaa atgtagaaga ttaccgagtc ttcttcgagc
      301 tttatctttt tggtaacttc tgcttttagt ttttcgtatt tagcatcggt gagttctcct
      361 tctaaaactg agttttgcac ccagttcaaa tatttccgag caatatttaa gaccttggtt
      421 actctacttt gatttacgtc ataaaccata atgataaaca tttaccattc agccccaaaa
      481 ggagcataat tttcttcccc cataaagtgt ttttccagct tatataattc cagccggatg
      541 agccttcggt aagagacctg tcttcctatt ttgctgtgat tgatagtagt ttttaatctt
      601 tcttcgaatc tttctacgaa agctcgtcgg cccgattcgt ctaaatatat cccccctact
      661 cgttcatcaa aattctccga gcctatcatc tttttgttca acaggtaaaa aataacccgg
      721 tcaaccaata tgggtttaaa gatttcagct acgtctaaat tcaaggtaaa tctacgaaag
      781 ttagtggtgt gcaggtatcc aatacggggg tcaagatgag ttttgtaaat ctcagacaac
      841 acaatagtgt aaagcagaga attgccaaaa ctgataaggg cattcattct gttttctggt
      901 ggtcttttgg tccggctttc cagattaaaa tcttcgtctt gcactataga gtcaaaagaa
      961 gaatagtaaa tctgccgggc attcccttct atagccatta acgattcaac tgtttcttgc
     1021 tcctctgccg attgagaaaa ggaatttatc ttttctatta atggcgatag ctcaactcct
     1081 cgattgttgt aatagctcaa gacttttccc atattctgaa gggcaccttg aacaaagctt
     1141 ttagctaaag tcagccgctt tgcttcatcc agataatgct ctgcttgttt caagatgaga
     1201 taaccagagt tgtagtgctc ccgagggtaa aagctccccg aataatagcc ataataatta
     1261 taaaaatgga gcaatatttc attttgagag agatattcga gtatctcttt attgagagaa
     1321 acttctccaa agatatgaat ttctcgagta ttctcgatag ggacatattt gcgtcctccc
     1381 tcactatctt caaaatagat ggtgttatgt tttcgctcga gtttaccact ggagaaaatg
     1441 taaatgctct cactcatgcc caacaaaact cccgataggc acaattggtg caaaacttta
     1501 tttttcgagg agggggagaa gaaggtagag caattatcct ttttatatct tctataacat
     1561 ttttcatctt ttcttctaat tctggagtta agtctacttt tattcttttt ctttctttag
     1621 gaaaaagtaa aactccttct gaggtgatgc cactctctcg taatcggtaa aggtaaaaca
     1681 acagctgtag cctggcactt tcttcggctc gagaactctt ttttatttct cccaccacta
     1741 aatttctctc tttagtttga accaggtcta agacgacatt ttcaaagtgg atttttttct
     1801 tatcccggtc gtagctttcc gtgtctaaaa atctacctat ttccagatag gaattatcct
     1861 ggtcaggggt aatttgatga gccataagcc atagttgcct ctggcaaata gagtagcttt
     1921 gaaccagagt tcccgataca tggccatcta ccatatagct gaccctcctt ttaatttaaa
     1981 gccagtttcg gagtcataga aatcggaaag actgtcatta ttaacatagc gaaaaccgga
     2041 aacttcaggg gggagatttt ctacctttat tggtactgaa acgacgaatt ggtaaaaatc
     2101 gccttttatt ttatcaaact ccagacgtct ttgccacagg tcttttattt ccttgatttc
     2161 taaaaaggca ttccagactt cctgagcttc ctcgtttatc tctaagaaaa cgtctaattt
     2221 gggataatct tcctcaatca gttgaaaatc tgctatcccc acctgctggt cattgctggt
     2281 gtattttaat cgataaacag cttccagtag ctttctcgat tcatcgctcg attttttatt
     2341 ttgcaatagg tgaaaataat tctccaccat ctcgaataaa tcttgttcat aaagaacatt
     2401 atttttaaga agaacttttt ctgtttcccg gagaagtaaa gaatcataga tataggaaga
     2461 gtaggcacgt ttattatctt gcaagcgtac tatatttacc agcccctttt ctttgctcca
     2521 gttccggtta catcttccag cagattggat gagagaatcc agaggagcga tgtcccggta
     2581 aaccacatca aaatctatat ctactcctgc ttctaccagc tgagtggtga cagccaagcg
     2641 aaatttcttc tcctgtagtg cttttatgcg cttcaagcgt tcccagggga caacatgggt
     2701 agaaaggtag actattttat ctttaacctt ttccctcatc aactgataga gcgtagtagc
     2761 agcatttatg gtgttcataa tgaaaagata gctctggtct tcttttatag tcagatagtt
     2821 gaccagctct tctatggtca tactttgctc tatatcggag cggatagtaa ttcgattgac
     2881 ttgggggaaa tacttctgag ggtcaaccag agggaacatt tgtcctcttt ctaaaatcaa
     2941 aggctgagta gcagtcacca ggattatata ggcattgaac gatgaagtta gatgcttaaa
     3001 aacttctctc agaagcaacc aatatttgaa aggtatagac tggacttcgt ctaagatgat
     3061 aatggagcct gctaaccgat ggagtttgcg tagggtggag tttcgatggg aaattaagct
     3121 atggaaaaac tgaacaaaag tggtaacgat aatttcagcg ttccatccct ctattaaaat
     3181 cttagcctgg tcaggttcaa actctttttc atcttcctga tagtaaatat ccgacaaatg
     3241 gtggtgcttt aagatgatag agctatctac tgcaagacca ctatttttca aaattttttc
     3301 tatctggtca gcattttgct caataatact taaaaaggga agagaatata tgatacgggg
     3361 agtgtaacct ttttcttctt ttattttttc tcgaagcttc agagcaaaag caaaggaggt
     3421 aaaagttttc cccaacccag tgggaaggtc taaactaaaa aatcgggtag ctaaatctat
     3481 ttcctgatta attacttcct gataagcttc ttctcttaac tgatttaaat aggtttctcc
     3541 ccttttcaga gtagatttat actcacttac tatagaagga ggaatactca cttcttttct
     3601 ggatagttca cccacagtta cttcgctttt atcagcatca attaacagag aaaatactaa
     3661 attggtcaag aaataccagc tgagattatt ttccttactc agctggcgca atgttcttct
     3721 catctttatc acttctgaag ggaaagcttc cacccatccc gaccaggagt ctctggtaat
     3781 gcctgatgga agaccagaga ttttaaggtt ttctaccagt atagaaagcc ggtcggcatc
     3841 tatatcttct atttgtttta aaagaagttc ttcttcttta gtatccagaa aagattcagt
     3901 taaaacatct tccagattcc cgtgatgtct tttaaccgcc aggaagccca caaaagccaa
     3961 gagctttttt tggtctgcgg acaaatgagt tgcttctcga aattcatctt ccaccagaaa
     4021 aaaagtggct atcgccgaga gcagagagtg acgagtttcc ttagatttcc cgggattttc
     4081 tgaaagaagg tatttctgaa aagcattagt agacttaccc aggtcatgag ctaaaccaca
     4141 gattttgact aacctggtca aatcactttt agagaattct cccaaagtat ttataggagc
     4201 ggcttcccag ttggtcagtg ctattttagc tacccggatt aagtgttctt ctaaatattt
     4261 cccggggtga gaatagagat tagaaaaaga gaagccctcc actcccctca ccttcccagc
     4321 tcttttgggt ttcaacccgg ataggtttac cttcggattc aaagataact tcttgatatt
     4381 cttctactac cctctcctca ttcatccgaa gcgggattct ctctttaaaa tatttcttcc
     4441 cttcttcaaa atttatccgc aaaccctcca agtaagaaag gggaatgaca gtaaccaaac
     4501 tcgtttcccc ttcttttctt ctttcttcta cctcaagtac cttttgatat tggaaatcag
     4561 ccagaagttc actcaaacct aaagcaggag tgaacactgc ttcgtggttt ttgacttttc
     4621 tcaccaggtc ttcaaaaatt gcctgatcct ggtgagaaac atataatcga taaccggggt
     4681 cttttacaaa ttcagtccgg atttgggttc tcgctccatg gctccctctt ttagtcggta
     4741 tccagtagtt ttgcttggta tttatatgat taagacctaa gcgaattttt ttgaccggcc
     4801 tcaggatttg aagaccaatt ttacaatggt caccagagaa gacccgaagg taatcttttt
     4861 tgtccacccc gattatagct cccagcatcc ccttaatcgt agaaggagga ggaaaaggga
     4921 aagtcaaagg agaagaagta gtataaaatt tccgaaagtg agctaaatca ccataaatat
     4981 caaaaaccag gactttcatt agttacccac cttttaaaaa gggaaggatg cagtcggcaa
     5041 ccccgagaaa gcactttcca ctcttaccgg ctctccatct ttagctagga ctatccggtc
     5101 atcagttttt atccttaact tttcaatttt atcctgattt tcctgcaaag ctttaatcaa
     5161 gggagagatt tccacctgca attcagaaat atctctgatt ttttcttctt ctttttcagt
     5221 ctgaatagct attcgtttat ccagctctcc gatatagaaa ttccctccct tatagattat
     5281 ctgcaagaga aaacggggag tttgtccaaa cttagagcca gagatgagat ttttagtgcc
     5341 gttccagatg gcttccatca tcaaatccac atcctcacaa gttaaaggaa ggttttgagc
     5401 ttgagccgct ttttcattaa ccactcccca aaaggcgatt aaagaataag ggagaatata
     5461 tctctcggtg aaagtgcctt gttttttatc agattgagaa ggcatcacag tagtcccttt
     5521 cacataggtt aaatctactc gatggagaga cctcccatac ttaaattgaa caggaccggt
     5581 aagagccata gtctgctctt ttactgcagt agtagctcca aaaagccgga tatcaatgca
     5641 tttctctatc aaatcctgag gagagctaaa atctttaagc cggtcttcct tggtttttaa
     5701 attgccattg tcatccctta tttcccgcac aaaaacctct tttcccagat aatcgtaaag
     5761 atagtctctg atagtcctct ttaatctgac atcagagacc agattaatcc cggtttcttc
     5821 atcaattctg ggtttattct catctaccgg gtcaccattg gggttagcgt tggtaacatc
     5881 ataaagaaaa agaatttctg aacggttttg aataacatcc atctcaaaca tcctcctcta
     5941 ccagaatttt ttctttctca cttttttcgg aataaataaa aggagtgact tcagaaagga
     6001 gattcattcc ggccacaaaa tagaagttca tttcatctgt ggacatcttc cagttatttc
     6061 cggcttctaa aagatagtga gccgcttctc tggctaactt ctgtttcccc tggtcaaaag
     6121 aatcatattc ctgcagctta ttctctaccc ggggaagcaa tcctaaaaag tctcgctcat
     6181 ccatcctcaa ccccttgaga ttctttagaa aaggtggggt ggactctctt ctattatatt
     6241 gaactcggag aagaagctga gttagagcac cgagaagaaa gagacctctt ttgagggggg
     6301 tctcaaaagt agcaccatac tttttaaaaa gaggatcaaa aagtctttcg tccattgcca
     6361 ttacctccat tttgattaat ttcatcctct ccagaaaatt aaccaccatc actccctcta
     6421 tagtggtcgg gccaaaatat tggtcgttta caaaactgct gcgaatctta ttcatcaaga
     6481 aatgcaagat aaaatcgtaa tctaccggcc ttcctttaaa cactctatct actaagtcca
     6541 gaaaataacc gtctaaatcg ttatctcttt tagccgggtc agatttagaa aaaaagttgc
     6601 ggatagtgcg gaaggtgaaa ttttgctgga aagcgtggtc tatctcgtct ttgacctcga
     6661 atatttcctt taggtgagaa gggaaaacgt cttctataaa ggctaaaatc ctttctgcac
     6721 tttgctgctt gagaagaaaa agaaaattaa gagtaagatt atcctgagct tgagaaagaa
     6781 gtagaagaat ttcttgctca tctcctaaaa atctattctg gctttccttt tttaagctga
     6841 gcaacttagg agaatcggta aacaggtcta aaacatcctg agccacattc tcttttccca
     6901 taatgaattt gggaatgagc tggtagcgga gaccggcaaa attaaaagtc aaattagctt
     6961 ctaaatactt tttcccctct tctaaggctt gtttacaatc taagcagata ggaaaatttt
     7021 tccaggctac ttcttcccgg aagcctccgg cgataaaccc cggtttatct atggtaaaaa
     7081 agctgtacac tccgtctcct aagataacgt cttttttctc attgcagata gaacaagtag
     7141 cgaactgtcc tcctcttttt ttattacccc cttgaactcc caaggtaaaa atctctctaa
     7201 aaagaggaat atctccggga taatattctt cgttttcatc tcttaatttc agagtgataa
     7261 aaatgccttc ttttctggga atctcttccc taattttctg gatttctcct attatccggt
     7321 ctctatgtgc ttctaactct ctctttagag aatgaagtaa ctctttttcc tccggggaaa
     7381 ggtctgcatt cttttggaga actctgttgg cccaacccag gattttattg gtaaaggttt
     7441 tctgtggttc ggtaagcttg gcagtaggag aaaagtccgg gccatttcct gctcctgagc
     7501 gatacaggta tctcattttt ttagaactat catattcttc tctatccacc tcctcaaagg
     7561 aaacctgatt gcctctcttt tctaaaatta tggtaaccac ctgagtatac tttccgttaa
     7621 tgttggggtc ttccaccata atctccatca ggtcttttct ttctctctga atgatgattt
     7681 cccctatttc tttgattgcc tcaatcatat ctttcctcct tcctcgtttc cacccttact
     7741 ccacccgagc tatctaaaag gtaaaaggtt gtcagatttg agctttcttc gatgatggtg
     7801 tagtgtgata ggagggctat aacataataa attaagtttc ggattgaata accgcactaa
     7861 aaagagattt ttctagttca gaattcttgg ccggatagct caaacccccc ttattatttc
     7921 ctcatatcgt aatatagcac atttgagcta aatttccacc tctattcccc ggagatggcc
     7981 gaagctctgg cacaggaaag agggcaaaat gacgcaaaat ttctcttttt tcattaccac
     8041 atttataaat aaatttaaca tcagcttaat ttactcttaa aaaacttctc ttaagctgga
     8101 gaaaatgaaa aggaggtagt tttatgaaaa ggagtctttt aatcttggcc gtccttggaa
     8161 tatgcttggg ctcattgtta gcagtgtgct cagtggaggc acaagctgtt cctccaccaa
     8221 agcccacgct taaatttaat tctcagggca cttttaagat tgttatgttt tcagatgtgc
     8281 aggaccaatt tcccattaat cctcagacca tagaagctat gaacaatatt ttagacttcg
     8341 aaagaccgga atttgtgata ctggtggggg ataattgtac tggagctatc ggaagcgca
//
LOCUS       DAOSVE010000077         8247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_4462_length_8247_cov_77.485352, whole genome shotgun sequence.
ACCESSION   DAOSVE010000077 DAOSVE010000000
VERSION     DAOSVE010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 8247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8247
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4462_length_8247_cov_77.485352"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            373..448
                     /locus_tag="PK016_06755"
     tRNA            373..448
                     /locus_tag="PK016_06755"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:406..408,aa:Lys,seq:ctt)
     gene            1162..1287
                     /locus_tag="PK016_06760"
     CDS             1162..1287
                     /locus_tag="PK016_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02796.1"
                     /translation="MVRVKNNQIALSNYRNILIISLSGKTSSSGGKKEKDPAGKD"
     gene            1293..1526
                     /locus_tag="PK016_06765"
     CDS             1293..1526
                     /locus_tag="PK016_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02797.1"
                     /translation="MSEEKSLKELEKRVWDSFSEAIFLRDLAQRLEMEIADVWEAILH
                     MEEEGLISFYNWQCKTVIYKKGREEPCLKNSSW"
     gene            1502..1996
                     /locus_tag="PK016_06770"
     CDS             1502..1996
                     /locus_tag="PK016_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HXL02798.1"
                     /translation="MPKEFQLVAFQLGEETYGVDISQVEEIIRMQPITRVPGAPDFVE
                     GVINLRGRVIPVIDLRKRFRLPPREETKNTRIVVVEVSPNTVGMIVDAVDEVLRISEE
                     KVEAPSTLISSIDTEYIKGVGKLDNKLLILLDLSKVLTKEEKGVLQSMKEEIEAEKEG
                     ETEK"
     gene            1993..3498
                     /locus_tag="PK016_06775"
     CDS             1993..3498
                     /locus_tag="PK016_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HXL02799.1"
                     /translation="MTLSLQGKLTSGFGLLIVILLIVVGLGYYAINEVYKAFYALPEL
                     QNYRETSQEVNAIITSIDRIQNILLLVGLIALVVGVVSALLIYRSILKPIRKLARSCE
                     MVEKGDLRQEIEVKSEDEIGKVARGFNSMVQRLRELISQVLRISSNLASSGEELSSSI
                     EEISKATEEIAKTVSQVAQGSTEQSTELETINQETQKISQIAHQLSEATQRNLNLIAE
                     MKKGLEKNQLSLSEIEQAMKVTSTEGENSEKEARKGQELLSLLSQNIQAISQVTQEVA
                     QSISTLEARSQEISKIVDLITGIAEETNLLALNAAIEAARAGDAGRGFAVVANEVRKL
                     AEESAQAAQQISTLIGEVKNDTQMAVERMERAEDRVKEGVKESEEVAKNFQNILITIQ
                     KVIQSVNNLNLVFEEAKNSQKITEKSAEEVAVLSEDNAQMIEEINSRIQGVSKSINSV
                     ALVSEENAASSQEVSAAAEEQSASLEELTSASQSLAQLAQELRELVATFQV"
     gene            complement(3609..4844)
                     /locus_tag="PK016_06780"
     CDS             complement(3609..4844)
                     /locus_tag="PK016_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXL02800.1"
                     /translation="MPAYLYRVRDKTGKLLEGKIEAPSEQEAAAILRREGVFITSLEE
                     ERAAKSALQREIKLGGGGKKVELQDLAVFARGISTMLEAGVPLLQALRGMADQATNRY
                     LAQVIGEVAQKVARGYSFYQALSDYPKVFNRVFVGMVQSGESGGNLDWSLQRLADYLE
                     WEKDLRDKIQSATYYPLILIVAIIGISLFMVYFVFPQFVVLFEGFQMELPSLTKFMLG
                     VMNFLGQNWLLVYGSLGGAILAFILYINSEQGKRWWDRHQPKLPLFGQLFHKLLVSRF
                     CWLLSGLLRSGMPMVQALEVTASSVGNYYVGDIIQKIAEDIRRGSNLSQGVAIHSFFP
                     PLVVQMIAVGEETGSLELTLGKVTELYDKEIKIFVDRISTIIEPILTVIIGGAVLLLA
                     LALFLPIFQMASQGMSAGF"
     gene            complement(4846..5913)
                     /locus_tag="PK016_06785"
     CDS             complement(4846..5913)
                     /locus_tag="PK016_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HXL02801.1"
                     /translation="MNIEISDLLKESVERGASDLHLTAGVSPTLRIDGKLIPLDYPPL
                     TPEDTERFAWSLAGEERFSAFSREKELDFSLGISQFARFRINIYQQRGSVALAIRALN
                     YAIPSMEELRLPASTLQKLASLSRGLILVTGPTGSGKSTTLASMIEYINNTRSCHIIT
                     MEDPIEYLHHHRKSIINQREVSSDTLSFENALVHALREDPDVILVGEMRNLETIALAL
                     TAAETGHLVFATLHTGNAPQSIDRIVDVFPPHQQTQVRVQLAGCVQGIISQQLLPLIN
                     GKGRVPAVEIMIANNAIRNLIREGKSYQIYNIMQTSLSEGMITMDKALLDLYRDGQVS
                     WEEAQARSIDPREFSQMRSRI"
     gene            complement(5923..7647)
                     /locus_tag="PK016_06790"
     CDS             complement(5923..7647)
                     /locus_tag="PK016_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HXL02802.1"
                     /translation="MPENKNTNIKIPGFQRLGEIMLSRQYITPQQLEEALKVQKETGE
                     KLGVVLKKMGLVGTKEVYQALAEQLGASFIELDNYLIDPQVASLLPEKFCRERQVIPV
                     GEENGALILVMANPADVVTIDRAQLMTGKEIKPAIAPPEEIERIINTYFGAKESVDDI
                     LRGVEEKEGVALEEKAKEIEIGQLKALGEEAPIIQAVNAFILQAIRAGASDIHLEPQD
                     KIVRIRFRIDGILHDISSTSSRVHPALVSRIKILSRMNIAERRLPQDGRFQIRVDNRI
                     VDFRVSSLPTIFGEKIVMRILDKSTLLLDLDKLGFGPNDLEKFTRLIKHPYGMVLLTG
                     PTGSGKTTTLYSALNLINQPELNIITIEDPVEYQLPGINQVQINPKIDLSFASALRSI
                     MRQDPDIIMVGEIRDRETAEIAIHAALTGHLVLSTLHTNSAAGAIIRLQEMGVPSYLI
                     SSALLGVVAQRLVRKICLHCRTDLEIEGETALKLTEGGKEKLILHRGQGCNLCNRTGY
                     RGREAINEVLVIDDDIRSLILKNATEGEITTKAKEKGMSTLKENAIQKVLKGITTLEE
                     MYRVVANF"
BASE COUNT         2342 a         1688 c         1761 g         2456 t
ORIGIN      
        1 cccccaaaag caaaaaacat aaaaagcaaa aacccaccga ggaaggaaaa gcacttcctc
       61 ggtgcaaggg ggaattcttt tcccccttgc caaccccttt ctttttcttc cctcgctttc
      121 aatggaagaa ccccacatat tccttccctc gccctcgact ccctttcctt gtcgtcgcga
      181 gggtcgtaaa aaacagccta cggcgtaaga ttaaaaaaat agaaaatccc tcccatcttt
      241 tcaatcctat tagagggatt atagcttaag gggtgggtca tttttattcc ggctttcctg
      301 ggtcaattat acgccggcgg tgacatgtgt tgacatcttt atttatccat agtatattga
      361 taaccactga atgggccgtt agctcaattg gcagagcaac tgactcttaa tcagtaggtt
      421 gcaggttcaa ttcctgcacg gcccaccagt cttatgttac tttactgcag ggaatcctct
      481 gatttttagc atagagttac ttttcaccgg ttcagtggtc aatgagaact actttttctt
      541 ttgagaaaac gataacttaa aatgctatcc tgctagtatt aagtgggttt ttagtttgct
      601 taggaagttt tgagttaggc tatgtcttat atttgtgtgt ttggtagaat agtgttacct
      661 ctcacattat tttagacaga gggggccgat tatgtcctat atcctcgtaa aaatagaaaa
      721 gtggatggcc attgtggtaa agtaaatttt atggaaaatt ttacagaaag gagaccatct
      781 acttatgaga aggataccaa agagtgaagt attacacaag gagttgaatc aaatattata
      841 gtattccgca aaactggtta aaaattttag gaagtaaatt atatcggaag tcaaaaactc
      901 aaggtcgcta tgtactgagg gatactataa tttttctatt agccttttgt ttctttgttc
      961 tttgtctttt tctttttctt tttctttttc tttttctttt tctttttctt tttcttttgt
     1021 tttttggggg attataaaga ggttctcccc tttatagttg ttgtttctgt ctcatatttc
     1081 tttttcagag aaaaagtctc tcagcctcta aagttttgga agaaactgcc gataatattg
     1141 taagaggagg caaaaataaa tatggtacga gtgaaaaata accagatagc attatctaat
     1201 tatcgtaata tactcataat atctctttca ggaaaaactt cctcttcagg aggaaaaaaa
     1261 gaaaaagacc cagcaggaaa ggattagttt tagtgtctga ggaaaaatcg ctaaaagagt
     1321 tagaaaagag agtttgggac tctttttctg aagcgatatt cttaagagac ctggcccaga
     1381 ggttagaaat ggaaatagca gatgtttggg aggctattct tcatatggaa gaagagggat
     1441 taatttcttt ttataactgg caatgtaaga ccgtaatcta taaaaaaggg agggaagaac
     1501 cttgcctaaa gaattccagt tggtagcttt tcagttagga gaagagactt acggggtgga
     1561 tatctcccag gtggaagaaa ttattcgtat gcaacctatc accagggttc ctggagctcc
     1621 tgactttgta gagggagtga ttaacttgcg aggaagagta attccggtga ttgacctccg
     1681 gaagagattt cgccttcctc cccgggaaga aactaaaaat acgagaattg tagtggtaga
     1741 agtatcacct aatactgtgg gtatgattgt agatgctgtg gatgaggtat taagaattag
     1801 tgaggaaaaa gtagaagctc cttctaccct catctcttct atcgacaccg aatacataaa
     1861 aggtgtagga aaattagaca ataaacttct catccttcta gacctatcca aagtgttaac
     1921 caaagaagaa aaaggtgtct tgcagagcat gaaggaagaa atagaggcag aaaaggaagg
     1981 cgagacggaa aaatgacttt gtctctacaa gggaaattaa cttcggggtt tggtttattg
     2041 attgttattt tattgatagt agtaggattg ggttattacg ccattaatga agtatataaa
     2101 gctttttatg ctcttcctga gctgcaaaat tacagagaga cctcgcaaga agttaatgcc
     2161 atcatcacca gtatagatcg gattcagaat attctgcttt tagtaggtct tatagctttg
     2221 gttgtgggag tggtttctgc tcttcttatc tatcgcagca ttttaaaacc catacgtaag
     2281 ttagcccgga gctgtgaaat ggtggaaaaa ggagacctcc gtcaggaaat agaggtcaaa
     2341 agtgaagatg agatagggaa agtagctcgt ggttttaact ctatggttca aagactccgg
     2401 gagcttataa gtcaagtatt aaggatttct tctaatctgg cttcctcagg tgaggaactc
     2461 tcttcttcca ttgaggagat ttctaaagcc acagaagaga ttgctaaaac cgtttctcag
     2521 gtggcacagg gttctactga gcaaagcact gagttggaaa ctattaacca ggagacccag
     2581 aagatatctc aaattgctca tcagcttagt gaagctacac aaaggaatct taatttaata
     2641 gcagagatga aaaaaggatt ggaaaagaat cagctctctc tttctgaaat tgaacaggct
     2701 atgaaggtga cgagtactga aggagaaaat agcgaaaaag aagccaggaa aggtcaagag
     2761 ctcctctctc ttttatccca gaatattcag gccatctccc aggtaacgca agaagtagct
     2821 cagtctatct ccacgctgga agcgaggtct caagaaataa gtaaaattgt agatttaatc
     2881 actggaattg ctgaagaaac caacctttta gctttaaatg ctgcaattga agctgctcga
     2941 gcaggagatg caggacgggg atttgccgta gtagctaatg aggtcagaaa attggccgaa
     3001 gagtcagctc aagcagcaca gcagatttca actttgattg gagaggtaaa aaatgatacc
     3061 cagatggcag tggagaggat ggaaagagct gaagaccggg ttaaggaagg agtaaaagag
     3121 tcagaagaag tggcaaagaa tttccagaat attcttatta ctattcaaaa agtgatacag
     3181 agtgtcaaca atttaaatct ggtatttgaa gaagcgaaaa attctcaaaa gatcacggaa
     3241 aaaagtgctg aagaagtggc tgttctttct gaagataatg ctcagatgat tgaggagatc
     3301 aatagtcgta ttcaaggagt aagtaagagc ataaattcag tggctcttgt atcagaggaa
     3361 aacgctgctt cttcacaaga agtttccgct gctgctgaag agcaaagtgc ttccttagaa
     3421 gaattaactt ctgcttctca atctctggct cagttagctc aagagttaag agaactggta
     3481 gctaccttcc aggtatgagt tctatcccgg ggaaagcttt ccccgggctt ctgtttttta
     3541 gccgaatggt aaacgataat aagttcttcg ccatcccgtc agcctcgaga gtatctcttt
     3601 ttagagactt agaaaccagc ggacattccc tgtgaggcca tctggaaaat gggaagaaaa
     3661 agagcgagag cgaggagcag aacggctcct ccaattatca ccgtcaaaat aggctcaatt
     3721 atagtggaga tacggtcaac aaatatttta atctctttgt cgtaaagctc ggttacttta
     3781 cccagagtga gttccaagct tccagtttct tctcccactg ctatcatctg caccacaagg
     3841 ggtgggaaaa aagagtggat tgccactcct tgagagaggt tgctccctcg tcggatatct
     3901 tcagctattt tctggataat atctcccaca taataattgc ccactgagct tgccgtaact
     3961 tccagggctt gcaccatagg cattccactg cgcaggagtc cagataacaa ccagcaaaat
     4021 cgggaaacta gaagtttatg aaaaagctgt ccaaacaaag ggagtttggg ctgatggcgg
     4081 tcccaccatc ttttgccttg ctcagagtta atatacagaa taaaagccag aatcgctccc
     4141 cccagcgatc catagaccag taaccagttc tggccaagaa agttcatcac tcctagcata
     4201 aacttggtaa gagaggggag ttccatctga aaaccttcaa acagaaccac aaattgagga
     4261 aacacaaaat agaccataaa taaagagatg ccgattatag ctactatcaa aatcaaagga
     4321 taataagtgg ctgactggat tttatcccgg aggtcttttt cccactctaa gtagtcagct
     4381 aaccgctgta agctccagtc caagtttcct ccgctttctc ccgattgcac catgcccaca
     4441 aatacccggt tgaaaacttt aggataatca gatagagcct gataaaaaga gtaacccctt
     4501 gctaccttct gggcaacttc tccgattacc tgagctaaat atcgattggt ggcctggtca
     4561 gccatgcccc gcagcgcttg aagaagaggc actcctgcct caagcatggt ggagattcct
     4621 cgggcaaata cggctaaatc ttgaagttct accttttttc cgccccctcc cagctttatt
     4681 tctctttgta gggcagattt agcagccctt tcttcttcta aagaagttat aaaaaccccc
     4741 tctcgccgga ggatagctgc tgcttcttgc tcgctcgggg cttctatttt gccctccagt
     4801 aacttaccgg ttttatcacg aactcgatac agataggctg gcatgttata ttctgctcct
     4861 catttgagag aactctcgag gatcaataga gcgagcttgt gcttcttccc agctcacttg
     4921 accatcgcga tagaggtcta aaagagcttt gtccatagta atcatcccct ctgaaagaga
     4981 agtttgcata atattataaa tctggtaact tttcccttcc cgaataagat tacgaatggc
     5041 attattggct atcataattt ctactgccgg aactctacct tttccattta ttaggggcag
     5101 gagttgctga cttataattc cctggacgca tccagctaat tggacccgaa cctgggtttg
     5161 ttgatgaggt ggaaaaacat ccactatccg gtctatactc tgaggagcgt tgccagtatg
     5221 gagagtagca aacaccaggt ggccggtctc tgctgctgtt agggcaagag caatggtttc
     5281 taaatttctc atctctccca ccagtataac atcggggtct tctcgcaggg catgtaccag
     5341 agcgttctca aaagatagag tgtcagaact tacttctcgc tggtttataa tgctttttcg
     5401 gtggtgatga agatattcaa ttggatcttc catagttata atgtggcagg agcgagtatt
     5461 gttgatgtac tctatcatag aggccagagt agtggacttt ccacttccag taggcccggt
     5521 aactaaaatt aatccccggg agagagaggc cagcttctgc agagtagaag ctgggagacg
     5581 aagctcttcc atagaaggaa tggcatagtt taatgctcgt atggctaaag cgactgaacc
     5641 ccgttgctgg taaatgttta ttctaaatcg agcaaactga gaaatcccca gagaaaaatc
     5701 cagctctttc tctcttgaga aggcagagaa ccgttcttct ccagctaagg accaggcaaa
     5761 tctttcagta tcctcagggg taaggggagg atagtccagg ggaattaatt ttccatctat
     5821 tcttaaagta ggtgagacac ctgctgttaa atgaaggtcg gatgctcctc gttctactga
     5881 ttctttaagt agatctgata tctctatatt catttttcct ccttagaaat tggctaccac
     5941 ccgatacatt tcttccagag tggtaattcc ttttagtact ttttggatag cgttctcttt
     6001 taaagttgac attccctttt ctttggcttt tgtggtaatc tctccttccg tagcattttt
     6061 tagaattaag gatctgatat catcgtctat caccagaact tcgtttattg cttctctacc
     6121 tcgatagcca gttctgttac acaggttgca cccctgaccc cggtgcaaaa tcaatttttc
     6181 cttccctcct tcagttaact taagagcagt ttctccctct atttctaagt ctgtccggca
     6241 atgcaagcat atttttcgca ccagacgttg agctaccact cctaaaagag cagaagatat
     6301 aagataagaa ggaactccca tctcttgcag gcggataata gctccagcag cgctatttgt
     6361 atgaagggta gaaagaacca gatgtccagt gagagcggca tgaatggcaa tttcagcagt
     6421 ttctcggtcc cggatttctc ctaccatgat tatgtccggg tcttgacgca ttatagagcg
     6481 gagagcgctg gcaaaactga ggtcaatttt ggggtttatc tgtacctggt tgatacctgg
     6541 taattgatac tctaccgggt cttcgatggt tataatgttc agctccggtt gattgataag
     6601 gtttaaagct gaatatagag tagtggtttt tccacttcca gtagggccgg tgaggagaac
     6661 catcccgtag ggatgtttta tgagacgagt aaatttctcc aagtcattgg gaccaaaccc
     6721 cagtttatcc aggtctaaca ataaagtaga cttatccaga attctcatta ctattttttc
     6781 tccaaaaata gtaggtagag aagaaactcg gaagtccact atccggttgt ccactcggat
     6841 ctgaaaccta ccatcctggg gaagacggcg ttcagcaatg ttcattcggg agagaatttt
     6901 tattcgagaa actagagccg ggtggactcg agaggaagta gaggaaatat catgcaaaat
     6961 tccatcaatc cggaagcgaa tccgaactat tttgtcttga ggttctaagt gaatgtcgct
     7021 cgctcccgct cgaatagcct gaagaatgaa cgcattcacc gcctgaataa taggggcttc
     7081 ctctcccagg gcttttaact gaccgatttc tatttctttt gccttttctt ctaaagctac
     7141 tccttctttt tcctctaccc cccggagtat gtcatctact gactctttag caccgaaata
     7201 ggtatttata atccgctcaa tttcttcggg aggagcaatg gctggtttga tttctttccc
     7261 ggtcataagt tgagctcggt ctatagtcac tacgtctgct ggattggcca taactaaaat
     7321 taaagctccg ttttcctccc ctaccgggat tacctggcgc tctcggcaaa acttttcagg
     7381 aagaagggag gctacctgag ggtcgatgag ataattatca agctcgatga aagaagctcc
     7441 cagctgctct gcaagagcct ggtaaacttc tttagtgccc accaaaccca ttttttttaa
     7501 aaccacccct agtttctctc cagtttcttt ttgaactttt aaagcttctt ctaattgctg
     7561 gggggtaata tactggcgag aaagcattat ctctcctagt ctttgaaaac cgggaatttt
     7621 tatattcgta ttcttatttt cgggcattac gtcctctttt atctggtttt cttttatgag
     7681 ataggcctct attctatgaa gtgtagttta tgatagcaca caagaatcat tctttcaatc
     7741 cagaacagtc tgtaatatct tttgctacta cgagcttgta tttcctcctt gtttatagta
     7801 tcccacaaaa ctggttaaaa tattaacttt taaaaagtaa attacaccgg aagtcaaaaa
     7861 ctcaaggtcg ctacgcatag agaaagactt tgtctttctc tatggccttt atttcttttg
     7921 ttttttaagt tttcttaatc ttgcatcgca gactgccttt ttctccagcg tactggtgga
     7981 tattttccca ccagtagttt ttgtttattt gctttttaag tctttgttct tttcttttgc
     8041 ttttgggggg ctataagggg gctctgcccc tttatagttc ggattcctga tagaggcatt
     8101 caggaatgac ggctatgaga gacattccct ttatttcgtc attcccgaaa tcttcaatcg
     8161 ggaatcctcc taatctgtca tgcctgaaat cctttatcag gcatctacat cattcgtcat
     8221 tcccgagcgc tttaatcggg aatccaa
//
LOCUS       DAOSVE010000078         8106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_4549_length_8106_cov_70.777543, whole genome shotgun sequence.
ACCESSION   DAOSVE010000078 DAOSVE010000000
VERSION     DAOSVE010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 8106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8106
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4549_length_8106_cov_70.777543"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1198)
                     /gene="flgK"
                     /locus_tag="PK016_06795"
     CDS             complement(<1..1198)
                     /gene="flgK"
                     /locus_tag="PK016_06795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HXL02803.1"
                     /translation="MSNAFFGLEIAKKALQAQQAAMDVTGHNIANSNTVGYTRQSPKF
                     TQETVSSGGLFVLPHLRNLGAGVSITEVQRLRDKFIDLQIRQESRIGSYWKTVDDGLA
                     QIEVIFGEPQEGALSEIFDRFWNTWQELAKSPESQAARALVAETANLLSTAFNHTYNQ
                     FATQQQQLNEKVAIKVDEVNNYVQQIYDLNQQIVRLSLGGGNANDYKDQLDVLVDKLS
                     NVLDFQVSENSNGTYSLILQGRILVSDKEKRFLEVEANATTGFWEVKFQGEEQINLDY
                     QNGELKALFYLRDEVIEDYKDYLNDLAGGIISAVNMAHKQGYTLEEPPVKGGDFFIGS
                     DAATIALDSSIDADPNKIAASSTGAPGDGENALAVARLRDEAIINGSTPDDYYRGVVS
                     RLGVEKE"
     gene            complement(1221..1736)
                     /locus_tag="PK016_06800"
     CDS             complement(1221..1736)
                     /locus_tag="PK016_06800"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HXL02804.1"
                     /translation="MIALNDFLSHLLMVLGKELQLQNELLQAAEEKKDLLLKNHIEEL
                     VPLLEREMDLVTQVRDTEKQLSDVWKNMVEQCNLEPGELALSRIIELSPPDLALKFSE
                     IQIELKRVLKTLQEINLQNAILINDTLHYIDVVFSLLMGEEKNKFYSPLGKEEEKSAR
                     ESKGILINGII"
     gene            complement(1733..2053)
                     /gene="flgM"
                     /locus_tag="PK016_06805"
     CDS             complement(1733..2053)
                     /gene="flgM"
                     /locus_tag="PK016_06805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HXL02805.1"
                     /translation="MKISSNQMESILRIYVDKAKESKKVSSSPKKKEEGEGDKVFLSN
                     AGQEMVKLQQQYKKIPDVRADIVQELRARIEKGEYEVRGREVAEKMFAREIADFFIEG
                     GQDI"
     gene            complement(2112..2528)
                     /locus_tag="PK016_06810"
     CDS             complement(2112..2528)
                     /locus_tag="PK016_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HXL02806.1"
                     /translation="MSLAKCKRCGNLFNKVVDKNVCPACMEKEEEEFQKVRDFLKRYP
                     EKKLEEISEGTGVDKKVILDFIREGRLQLAEIEGEEGSALLVCERCGKPITTGRFCED
                     CRKKLAAILSPDEDKSSSRSPNFYFKDAIEKKKKEG"
     gene            complement(2515..2811)
                     /locus_tag="PK016_06815"
     CDS             complement(2515..2811)
                     /locus_tag="PK016_06815"
                     /inference="COORDINATES: protein motif:HMM:NF021638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HXL02807.1"
                     /translation="MRIENKDLTISLKPQEPSPQSVKKACQDFEAFFLGFVFKKAFQP
                     LFVSSSSPFFSQEEAWFREMWVDEVTKKVEESGGWGLSDMLYRRLVEENKGEFS"
     gene            complement(2909..4030)
                     /locus_tag="PK016_06820"
     CDS             complement(2909..4030)
                     /locus_tag="PK016_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HXL02808.1"
                     /translation="MKIKESLILFCLVLVIFLAVSPVRGETVRIKDITEVEGVRGNQL
                     VGYGLVVGLAGSGDSRNSLFTNQALANMLEKMGISVDSQAVRSRNVASVIVTAELPPF
                     VQEGEQIDVTVSSLGDAKTLQGGVLLLTPLKAVDGKVYAVAQGPLSIGGFNVGGGGGN
                     VVQQNHPTVGMIPGGGIVERTVETNFVDEVRGVFTLLLREPDFVTASRIVQAINEELG
                     GGKAQALDANRVEVTIPEHYQHQVSRLIALVGEIPVEPDVSARVVINERTGTVVIGGN
                     VRILPVALSHGNLTVTITTEYQVSQPPPFSSGETVVVPQQEIKVEEEPARLFRVESGN
                     TIDDLVKALNALGVTPRDLVAILQAIKKAGALQGELIIE"
     gene            complement(4043..4648)
                     /locus_tag="PK016_06825"
     CDS             complement(4043..4648)
                     /locus_tag="PK016_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HXL02809.1"
                     /translation="MKRTVIILFMLVFVSLFLFPEGARGESLWQDEGWFSDPYSDYRA
                     SQVGDTITVIINEDTSGKNSASSTNNKQTSIGLKAGQGIFDFVPPATLESSNSRGGSR
                     DYGRDISLSGTITCQVTEVLEGGNLRIAGSKDVYLNKERETLSIEGIVNPRYIGADNS
                     VYSTQVVDAVIKLDGTLKPEKKSGLIGLLEGIFGSVMDILF"
     gene            complement(4645..5628)
                     /gene="flgA"
                     /locus_tag="PK016_06830"
     CDS             complement(4645..5628)
                     /gene="flgA"
                     /locus_tag="PK016_06830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HXL02810.1"
                     /translation="MRKKIVEYCFLWLLTFICLISVEEVKALTLEVNLKEKVEVSAES
                     ITLGKIAEISYPEREWQERLENLDLGYLPPPGEEREIKPEEIYERVVAQKVSQLDYIY
                     FSGAPTCRVIVKGELITRDELERKIREQLSEIFPFAQKIEVNLSSPGEVIIPPDGEIN
                     IIFSSPSSLKPWGVGNGTLEIRQNGRVEKTLPLRFDIQVYREVVRAKNKLSRGEVISK
                     DQIYSSLEVLDYRNEKAFSNIEEVEGKETKREIPAGAILIPSSLQEETLIKRGDLVTM
                     VAKYGGIVVTGTGKARGEGSMGDVIVVENPDSKKRFEAEVIGERMVEVVVK"
     gene            complement(5918..6706)
                     /gene="flgG"
                     /locus_tag="PK016_06835"
     CDS             complement(5918..6706)
                     /gene="flgG"
                     /locus_tag="PK016_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HXL02811.1"
                     /translation="MIRALWTAATGMQAKQMDMDVIANNLANVNTTGFKKSRVDFQDL
                     MYQVVRVKGTPTSADTQVPSGIEIGLGVRPGAVQKMFSQGNIYETSNPLDVVIEGDGF
                     FQILTPDGTIAYTRDGSFKLDAQGRMVTSDGFPLEPPITIPEGAESVTIAQDGTVTVK
                     MPGEMEPQEVGVIELARFINPAGLLSMGRNLYLATASSGEPQIGTPGLEGFGTLAQGF
                     LETSNVQIVEEMVKMITAQRAYEINSKAITTADEMMSIANNLKR"
     gene            complement(6754..7503)
                     /gene="flgF"
                     /locus_tag="PK016_06840"
     CDS             complement(6754..7503)
                     /gene="flgF"
                     /locus_tag="PK016_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HXL02812.1"
                     /translation="MIRGIYTATSALNLQELKQETWANNIANLDTPGFKKELTWAEAA
                     QEANLFRFLSSQNPYPLGEMTAGVQPGTEKITDFSPGRLEETGNSLDLAIEGDGFFVV
                     STPSGEAYTRAGNFQLDGEGRLVTVSGYPVMGEGGEIILPDRGTVSVDEQGNILVDGE
                     EIEQLRIVDFANRSAIRKSGENLFIAEGVAPQETTNFNLRQGFLEKSNLDPVEAMVTM
                     MEALREYEIAQRMLVSQDEALQKAVNNITQL"
BASE COUNT         2321 a         2014 c         1232 g         2539 t
ORIGIN      
        1 cttctttttc tactcccagc cgagaaacca ctcctcgata atagtcatcc ggagtggagc
       61 catttattat agcttcatct cgcaggcgag caacagccaa agcattttcc ccatctccag
      121 gagcaccggt tgaagaagca gcaattttat tggggtcagc atcaatggaa gagtcaagag
      181 caatggtagc tgcatccgaa ccaataaaaa aatctcctcc ttttactggt ggttcttcca
      241 gagtatagcc ctgcttgtgt gccatattta ccgcactaat aattccccca gccaaatcat
      301 tcaaataatc tttgtaatcc tctattacct cgtcacggag ataaaaaaga gctttcagtt
      361 ctccattttg ataatctaaa ttaatttgtt cttctccttg gaatttaact tcccaaaaac
      421 cggtagtagc attagcttct acttctaaaa atctcttttc cttatcactc actaatattc
      481 taccctggag aatcaaactg taagttccat tagaattttc actcacttgg aagtctaaaa
      541 cattggaaag cttatccacc agaacatcta actggtcttt ataatcgttg gcatttcctc
      601 ctcctaaact cagccgcaca atttgttgat ttaaatcata aatttgctga acgtaattat
      661 ttacttcatc tacctttata gctacttttt cgtttagttg ttgttgctga gtagcaaact
      721 gattataagt atgattaaaa gctgtactca aaagatttgc agtttcagct actaaagctc
      781 gagcagcctg agattcggga gacttggcta actcctgcca ggtattccaa aaacggtcaa
      841 aaatctctga tagagctcct tcttgcggct ctccaaaaat cacttcaatc tgcgctaaac
      901 catcatctac ggtcttccaa taagaaccaa tacgagattc ctgccgaatt tgcaaatcta
      961 taaacttatc tcgtaaacgt tgaacctcag ttattgaaac tccagcacct aaattgcgta
     1021 aatgaggaag aacaaaaaga ccccctgaag aaactgtttc ttgagtaaac ttaggtgatt
     1081 ggcgagtata acctacggta ttagaattag caatattgtg gccggtaaca tccatagccg
     1141 cctgttgagc ttggagagct tttttagcta tttctagtcc aaaaaaggca ttactcattt
     1201 ctttcccctt ccatttttta ttatattatc ccgttaatta aaatcccttt actttctctg
     1261 gctgattttt cttcttcttt tcccagtgga gaataaaatt tatttttctc ttctcccatc
     1321 aaaagagaaa aaactacatc aatatagtgt agagtatcat taatcaatat ggcattctgt
     1381 aaattaattt cctgtagtgt ttttagtact ctcttcaatt ctatttgaat ttcactgaac
     1441 ttcagagcca gatcaggagg agaaagctca attatccggc ttaaagccaa ctctccaggt
     1501 tctaagttac attgttccac catatttttc caaacatcgc tcagctgttt ttccgtatcc
     1561 cgtacctggg taaccagatc catttctctc tccagaagag gaaccaattc ttctatgtgg
     1621 tttttcaaaa gcaaatcttt cttctcttcg gctgcttgca aaagctcatt ctgtagctgc
     1681 aactcctttc ccaaaaccat caaaaggtga gagagaaaat cattcaaagc tatcaaatat
     1741 cttgccctcc ctcaatgaaa aaatcagcaa tttcccgagc aaacatcttc tctgcaactt
     1801 ccctaccccg aacttcgtat tctcctttct ctattctggc tcttaactct tgaactatgt
     1861 cagctcttac atcaggaatc ttcttatact gttgctgcag ttttaccatt tcctgaccag
     1921 cattagaaag aaaaaccttg tctccttctc cctcttcttt ttttttggga gaagagctaa
     1981 cttttttact ctctttagcc ttgtctacat aaatccttaa aatactctcc atctggttgg
     2041 aggaaatttt catttctctc acttccttta cacctactca ctatattata tcggacaaat
     2101 ttctgatttc tttagccttc ttttttcttt ttttctatag catccttaaa ataaaaattg
     2161 ggggaacgag aagatgactt atcttcatca ggagagagaa tagcagcaag cttttttctg
     2221 caatcctcac aaaatctccc cgtggtaatg ggcttaccac atcgctcaca gacaagaaga
     2281 gcagatccct cttctccttc tatttcagcc aattgcagtc tcccttcccg gataaaatct
     2341 aaaattactt ttttatctac cccggtcccc tcgcttatct cttccaactt cttttctgga
     2401 tagcgtttca agaaatccct aaccttttga aattcttcct cttctttttc catacaagca
     2461 gggcaaacat ttttatctac cactttatta aacaaattac cgcacctttt acatttagct
     2521 aaactcacct ttattttcct ccaccaatct tctataaagc atatcagata atccccaacc
     2581 cccactttcc tccacctttt tagtaacttc atccacccac atctctctaa accaggcttc
     2641 ctcctggctg aaaaaaggag aggaagaaga gacaaaaaga ggttgaaaag cttttttgaa
     2701 aacaaacccc aggaagaaag cttcaaaatc ctggcaggct tttttcaccg attgaggaga
     2761 tggttcttga ggtttcagag aaatggttaa atctttattt tctatacgca taagctaccc
     2821 tctctatttt ttaaaaatat atcataatta actcacaatg caagcaccaa aaaactaaaa
     2881 tctttttcag gaaatcatta tccaaagtct actctataat taactccccc tgtaaagctc
     2941 cagccttttt gatagcctgc aaaatagcaa ccaggtctcg aggagtaact cctaaagcat
     3001 ttagagcttt aactaaatca tctatggtgt ttcctgattc tactctaaaa agacgagcag
     3061 gctcttcctc caccttgatt tcttgttgag gaaccactac agtttctcct gaagaaaaag
     3121 gaggaggctg agaaacctga tactcagttg taatagttac agttaaatta ccatgagaaa
     3181 gagctaccgg taatatccgg acattccctc caattactac tgttcctgtc ctctcattta
     3241 tcactacccg agcagagaca tcaggttcta ccgggatttc tcccaccaaa gctatcaatc
     3301 gagaaacctg gtgttgataa tgttcaggaa tggttacttc tactcgattg gcatccaatg
     3361 cttgagcttt tcctcctccc aattcctcat taatagcctg aacaatccga gaagcagtca
     3421 caaaatctgg ctctcgcaag agaagagtga aaacccctct cacctcatcg acaaaattag
     3481 tctctactgt cctttccact atccctcccc caggaatcat gcccaccgta ggatgatttt
     3541 gctgcaccac attgcctccc cctcctccca cattgaatcc tccaatagag aggggacctt
     3601 gagctacagc atacactttc ccgtctactg cctttaaagg agtgagaagt aatactcccc
     3661 cttgcaaagt cttagcatct cctaaagaag aaacggtaac atctatttgc tccccctctt
     3721 gaacaaaagg aggtagttca gcagtaacta tcactgaagc tacattcctg gagcgtaccg
     3781 cctgagaatc tacagatata cccattttct ccagcatgtt agccagagcc tgattggtaa
     3841 agagactgtt cctgctatct ccgcttcctg ccagacctac caccaatcca tatcccacta
     3901 attgattacc tcttactcct tccacctcgg taatatcttt tatcctcact gtttctcctc
     3961 taaccggaga aacagctaaa aaaattacta gaactaaaca aaataaaatt aagctttctt
     4021 ttattttcac ctctaccacc tattaaaaaa gaatatccat aactgaacca aaaataccct
     4081 ctaaaagacc tattaaacca cttttctttt ctggtttgag agttccatct aattttataa
     4141 ctgcatctac tacctgggta gaataaacac tattatctgc tcctatgtag cgaggattta
     4201 ctatcccttc aatacttaaa gtttcccttt ccttgtttag gtagacatct ttgcttccgg
     4261 caatccgtag atttcctccc tccagtactt ctgtaacctg acaggtaata gtgccagaaa
     4321 ggctgatatc gcgtccataa tctctacttc ctcctcgaga attgctactt tctaatgtcg
     4381 ctggaggaac aaaatcaaaa ataccttgcc ccgctttaag gccaatggaa gtttgcttat
     4441 tattggtgga ggaagcactg tttttaccac ttgtatcttc atttataatg actgttatag
     4501 tatctcccac ttgagaagct cggtaatcag aataagggtc ggaaaaccat ccttcatcct
     4561 gccatagaga ctcccctctc gctccttcag gaaaaaggaa aagagaaaca aagaccaaca
     4621 taaaaagtat aatcactgtt ctcttcattt caccaccacc tctaccatcc tctcccctat
     4681 aacttctgct tcaaatcttt tcttagaatc gggattttct accacaatta catcccccat
     4741 gctaccctct cctcgtgctt ttccggtccc agtaactaca ataccaccat attttgccac
     4801 catggtgact aaatctcctc gcttaataag agtttcctcc tggagagaag aaggaattaa
     4861 aattgcccca gcaggaattt ccctttttgt ttccttccct tccacttctt ctatgttaga
     4921 aaaggctttt tcgttgcggt aatctaaaac ttccagactg gaataaatct ggtctttact
     4981 tataacttct ccccgggaaa gcttgttttt agctcttacc acttcccgat aaacctgaat
     5041 atcaaaacga agaggaagag tcttttctac tctcccgttc tggcgaattt ccagagttcc
     5101 attccctacc ccccagggtt ttagagaaga aggagaggaa aaaattatat ttatttctcc
     5161 atcaggagga attatcactt ccccgggaga ggaaagatta acttctattt tctgagcaaa
     5221 aggaaaaatt tccgatagtt gctcccgtat tttcctttct aactcatctc tggttatcaa
     5281 ctcccctttg actatcactc tacaggtcgg agctccagaa aaatagatat aatccaactg
     5341 ggacactttt tgagctacta cccgctcgta tatttcttct ggctttatct ctcgctcttc
     5401 ccccggagga ggcaaatacc ccaggtctaa attctccaat cgttcctgcc actcccgttc
     5461 gggataggaa atttctgcta ttttcccgag agttatgctt tccgctgaaa cttctacttt
     5521 ttctttcaga ttgacttcca gcgtgagagc ctttacctcc tctacagaga tcaaacaaat
     5581 aaaggtcaaa agccataaaa aacaatactc aacaattttt tttctcattc ttccttatcc
     5641 ctcaacacca agtgtttccc ccagcataat tattccagat ttttatctat tctaccttct
     5701 ttcccttata ctaagggcga aagcatcccc tcagtatatt ttatgctttt tacgttttta
     5761 atctttttct ttttcatctt gcgccgcagg tttacctttt tattcctcat tagcattttg
     5821 tttgtttcca ccttttattt atctctttat ttttagggag ttataagggg gcagcgcccc
     5881 actgaggaag atgcttttct tcttcctcag ttattcttta ccgttttaaa ttattagcaa
     5941 tactcatcat ctcatcagca gtcgttatag ctttggagtt tatttcataa gcgcgttgag
     6001 cggtgatcat ctttaccatt tcttctacta tttgaacatt ggaggtttcc agaaaaccct
     6061 gagctaaagt tccaaaaccc tctaaaccag gagtaccaat ctgaggttct cccgaagaag
     6121 cagtggctaa atacaagttt cttcccatac ttaaaagacc agcagggtta ataaaccgtg
     6181 ccagctctat tactcccact tcttggggtt ccatttcccc tggcattttg accgtcactg
     6241 ttccatcttg agcaatagta accgattctg ccccctcagg aatggtaata ggaggctcaa
     6301 gaggaaaacc atcactggtc accattctcc cttgagcgtc taacttaaaa gaaccatctc
     6361 gggtataagc tatcgttcca tcaggcgtca aaatttgaaa aaaaccatct ccttcaatta
     6421 ctacatctaa ggggttagaa gtctcataaa tatttccctg agaaaacatt ttctgcactg
     6481 ctcctggcct tactcccaaa cctatttcaa ttccggaggg aacttgagta tcagcagaag
     6541 taggagttcc tttcactctt accacctgat acattaaatc ttgaaaatca actctgcttt
     6601 ttttaaaacc agtggtattt acgttagcta agttattagc aataacatcc atatccatct
     6661 gcttagcttg cattcccgtt gccgctgtcc aaagagctct aatcattaaa atccttcctt
     6721 tctcgggctc cccgcaggac cagccctaaa tttttaaagt tgagtaatat tgttaaccgc
     6781 tttttgcaaa gcctcatctt ggctaaccaa catacgctga gcaatttcat attcccgcaa
     6841 ggcttccatc atggttacca tcgcctctac tgggtcaaga ttggattttt ccaggaaacc
     6901 ctgtcggaga ttgaaattag tagtttcttg aggagctaca ccttctgcaa taaacaaatt
     6961 ttcaccactt tttctaatag ctgaacggtt tgcaaaatct actattctaa gctgttctat
     7021 ctcctctcca tctaccaaaa tattcccttg ctcatcaaca gaaacagtcc ctctatcagg
     7081 taaaataatc tccccaccct cacccatcac tggataaccg gatacagtaa ccaacctacc
     7141 ttctccatca agctggaaat tacctgcccg agtataagcc tcaccagagg gagtagaaac
     7201 tacaaaaaaa ccatctccct cgatggccaa atccagagag ttaccagttt cttctaatct
     7261 cccgggagaa aaatcagtaa tcttttctgt ccccggctgc actccagcag tcatttctcc
     7321 caaaggataa ggattttgag aactcaagaa tcggaataaa ttagcttctt gagcagcttc
     7381 agcccaggtc aattcctttt taaaaccagg ggtatccaga ttagcaatgt tattagccca
     7441 ggtttcttgc ttgagctctt gtaagttaag agctgatgta gcagtataaa ttccccgaat
     7501 caatactcta tcatctcctt tttttatttt tcacctacta tctctagata aatttatctg
     7561 ccaaatattt cattttcctg gaaaaagatt tttaaaattg aatatattta taactattcg
     7621 gtaattcctg tttttatact cttaggcttt tcttcccttt tttcaaaaca caggaaagct
     7681 ttatccccta tagtcggaaa tatagttaac cgcaaaacta attataatta ctataaaggg
     7741 gagaacccct ttataatccc ccaaaaagca aaagaaaaag acttaaagag gcaaaaacaa
     7801 ctgaggaaag aaaaacgctt tcctcagtgc ctgcggcact agagacgaaa gcaaaataga
     7861 tgaaagaaac taaaaccgat cacgctggag aaaagcactc cagcgttgag aaaggaaaag
     7921 cgtctttctc aatataaagg ggaaagaatt cccccttatg aatccccttt ctttttcttc
     7981 ccttgccttc aatggaagaa ccccacatgt tccttccctc gccctcgaag ggagagcttt
     8041 acatcttttt tccctcgccc tcgaagggag agggttaggg tgagggtgaa agctataaga
     8101 aagctt
//
LOCUS       DAOSVE010000079         7962 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_4631_length_7962_cov_75.932718, whole genome shotgun sequence.
ACCESSION   DAOSVE010000079 DAOSVE010000000
VERSION     DAOSVE010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 7962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7962
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4631_length_7962_cov_75.932718"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            474..549
                     /locus_tag="PK016_06845"
     tRNA            474..549
                     /locus_tag="PK016_06845"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:507..509,aa:Gly,seq:tcc)
     gene            641..889
                     /locus_tag="PK016_06850"
     CDS             641..889
                     /locus_tag="PK016_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02813.1"
                     /translation="MADLILIKVGNRAQNALKVQELLSRYGCNIKVRLGIHEFASECE
                     GEDEGIIILELSGNLEDFKKLKEDLEKIEGVKAIYEEL"
     gene            932..3406
                     /locus_tag="PK016_06855"
     CDS             932..3406
                     /locus_tag="PK016_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXL02814.1"
                     /translation="MESNIDLSQYRLSPDQLRWVCPPDAFSFECTDELEELEGFLGQE
                     RALSSIEFGLRMEKPGYNLFVVGPTGTGRASAVKNRIERLVKEKKEKGIIPEINDWCY
                     IFNFDDPDRPQVLKLPRGEGKVLSRLVDELLKNLRDAIPKTFAGDEYKNQKQILIDEQ
                     QRKNRQIIQELEKEALEEGFAFRMTSMGPILIPLVEGKPMTQEQYLALTDGEKEIIEN
                     RRQELLTRISEAFDKIHQLEVELKNKIAELDRRVAEFAINPLFKELVDKYSSSPEVLK
                     FLESLRNFTLAYLHIFRDSSEAPQQLPQLLPYQRLQDPFLPFKINVFVDNASLEDPPV
                     ILETHPNWTNLFGKIERRAFMGAYFSDHTMLKSGSLHQANGGYIIMYFRDLISNPGAW
                     EGLKRTLKNREIRLEDPFEQFGIIAPQGLRPDPLPFNAKVIIIGDEYYYRVLTLLDED
                     FRDLFKIKADFDYQIQREERILHSFACFVNNCCKEEGLLPFDRTGVAKVLEYGARAVS
                     HQKKLSSQFGFMKDILVEADFWAREEATDRVYARHVQKALQERKKRLSLMADRLQEAI
                     LEDVLLIDTQGKVTGQINGLAVYDLGDYSFGRPSRITARTYLGKQGVVNIERESRLSG
                     RIHDKGVLILGGYLGYKYAQNKPLSVAASVCFEQSYEGVEGDSASLAETCAVLSSLAE
                     VPIRQDIAVTGSINQKGEVQAIGGVNQKIEGFFRTAQEKGLTGEQGVIIPRSNIQNLM
                     LEEEVVEAVRKGKFHIYTVSTVDQAMEILTGLPAGERKPDGTFEEGTLNYLVDEKLVK
                     TVELLRSFEEKEGEKKEENISENKEE"
     gene            3434..3922
                     /locus_tag="PK016_06860"
                     /pseudo
     CDS             3434..3922
                     /locus_tag="PK016_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777384.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
     gene            complement(3962..4261)
                     /locus_tag="PK016_06865"
     CDS             complement(3962..4261)
                     /locus_tag="PK016_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HXL02815.1"
                     /translation="MSEEKTAKPSKTKDLGFYFLGLLSAKAWQYLGLVVHPETGEMLV
                     DLEEARKSIDLYSVILEKVKKDLEGSELRELEAQLSQLQLNFVEKSKEAKENKEQ"
     gene            complement(4258..5196)
                     /gene="miaA"
                     /locus_tag="PK016_06870"
     CDS             complement(4258..5196)
                     /gene="miaA"
                     /locus_tag="PK016_06870"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HXL02816.1"
                     /translation="MVQLKSRKLPAIVGPTATGKTDLSLALAERIGKVELIYADSLAV
                     YKYLDIGTDKPPVNFRERVPHHLVDFLDPGERWSAFQFRKEAIYLQREIRERGNLPLV
                     VGGTAFYLQALYRSFLFVGAPAENNIRIALERLSTEKLYALLKNVDPRRAQKIGKYDR
                     KRLIRALEIFKKTGHPPSLFSPRPSSFSPLLIGIRWDRDKLKERIYHRVGEMFKRGIV
                     EEVGELFSRGYSPPIPALDNFTYRPIVKLLRGDVSREEAQEEILQGTFRFLKTQINWF
                     KKAPVTWIEGEGRSLSILVEEAYTIIERNLVEEDTK"
     gene            complement(5177..6535)
                     /gene="miaB"
                     /locus_tag="PK016_06875"
     CDS             complement(5177..6535)
                     /gene="miaB"
                     /locus_tag="PK016_06875"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HXL02817.1"
                     /translation="MEIKRKPGFTAREVALATFWVETFGCQMNKSRSEHLAYQLQELG
                     FYLAEGPEEADIFVFNTCAVREHAAERAMNIIVKFAQERREKNPDSIIVVCGCLGQLE
                     REKLLGKDEKIDVLAGTHNWGKIPFLIKKAWQEKVKIVDFQEEAPSLYEERGYCHSSK
                     ISAYLPITFGCDNFCSYCVVPYTTGKERSKPREIIKEELMNLAEEGFREVTLLGQNVN
                     SYGRDRKEEAFEELLLDIEEWLGNKKIWVRFLTSHPRDFREKIVRIVKDSRVLCPYFH
                     LPLQAGSNKILQLMKRGYTREDYLNKVNLIRQSIPSARIGTDIIVGFPGETDSDFQET
                     LDVVREAQFDVAYTYVYSPRPGTAAYLLEDDVPLPEKKERLHVLNDTLRNIYREKLPS
                     LQGKVMEILVDRKEGGLLSGRGANNLRVYLREPCSMEVGEWIEVVLTPGEGNKVYGSV
                     KK"
     gene            complement(6553..6819)
                     /locus_tag="PK016_06880"
     CDS             complement(6553..6819)
                     /locus_tag="PK016_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HXL02818.1"
                     /translation="MDVLRVSSKSNPNAVAGALAGAIREKGIAEVQAVGAGAVNQAIK
                     AIAIARGYVAPSGIDLVCMPAFTDINIDGEERTAIKLVVKPRNA"
     gene            complement(6851..7624)
                     /locus_tag="PK016_06885"
     CDS             complement(6851..7624)
                     /locus_tag="PK016_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HXL02819.1"
                     /translation="MRFLIVGDVVGKPGRQILADKLKTIKEELDIEGVVVNGENAAGG
                     LGIVPEVCEEILSYGVDVITSGNHIWDKKEIVDYIDYQPRLIRPLNYPPGVPGKGWVM
                     VERKGKKWIVANMSGRVFMPSLDCPFHRIEEELENFKKVTNLILLDFHAEASSEKIAM
                     GWFLDGKVSCVFGTHTHVATADERILPQGTAYITDIGMTGAHDSVIGVKTEDILSRFL
                     TQMPIRYQVAKDNVKLNGIVVEVDEITGRATEISRLSIS"
     gene            complement(7640..>7962)
                     /locus_tag="PK016_06890"
     CDS             complement(7640..>7962)
                     /locus_tag="PK016_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541312.1"
                     /note="protein from Staphylococcus aureus has
                     phosphodiesterase activity against 2'-3'-cAMP and
                     2'-3'-cGMP; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HXL02820.1"
                     /translation="VEAVLVQAADAISASRPGARRESLESYIKRLEKLEKIADDFEGV
                     EKSYAIQAGREIRIIVKPDRIDDARATKLAYDIARKIEKELEYPGQIKITVIRETRSV
                     EYAK"
BASE COUNT         2301 a         1743 c         1595 g         2323 t
ORIGIN      
        1 tccgcaatat atatggtttt gaactaaaat gggatagatg ccaaccattg gtagagaacc
       61 tggaccgggt tttcagcgat ttgaaagagc aaattgataa gtttgaaagt ttcctgaatt
      121 ctattggata gaaaaaaaga tagtcaagcg tagtcctctc aatgcaatag gagaaaaacc
      181 tcttccgtca cccggtaatc tttagtcagg atctatttat ttaagaacaa aaactctgaa
      241 ttctcgacta aaatgacaaa acagacaatg agggggtaag ctccctcata acctcccaag
      301 gcaaatattt aaaaagtaaa taagcaaata ctactggtgg gaaaagctcc accagtataa
      361 ggggagacgc cttatgcatt tctaacatta tcacagacta ttgacaatgc tcttctctct
      421 ccccttgacg aagcttattc cattatttat aatcattgtg ctaacagtag cgagcgggaa
      481 tagctcagat ggtagagcgc cagccttcca agctgggtgt cgcgggttcg agtcccgttt
      541 cccgctccat tttttattct gagaactttc tccaaaaagt gtaaagagca aaaaatattc
      601 tataataaga acaattaaga ttcccaggga ggatgagagt atggctgatt taattctcat
      661 taaagtggga aacagggcac aaaatgcttt gaaagtgcaa gaattactaa gtcgttatgg
      721 atgtaacatt aaagttcgtt taggaattca tgaatttgct tcagagtgtg agggagaaga
      781 tgaaggtatc ataattttag agctttctgg caatttagaa gattttaaaa aacttaaaga
      841 agatctggaa aaaatagaag gagttaaagc tatctatgaa gaactgtagt aagaaatttt
      901 ctccttctat aagaaaacga agggaggacc tatggagagt aacatcgacc tttctcaata
      961 tcggttaagc ccagaccagc tgcgctgggt ttgtcctccg gatgcttttt cttttgagtg
     1021 taccgatgaa ttagaagaac tggaagggtt tttgggtcag gagagagccc tgagttctat
     1081 cgagtttgga ttacgcatgg aaaaaccggg gtacaatctt tttgtggtcg gtcctacagg
     1141 gacaggaaga gccagtgcag ttaaaaaccg catcgagaga ttggtaaaag aaaagaaaga
     1201 aaaaggaatt attccggaaa taaatgactg gtgctatatt tttaactttg atgacccgga
     1261 tcgtcctcaa gttttaaagc ttccccgggg agaagggaag gttctttctc gtttggtgga
     1321 tgagctatta aaaaaccttc gagatgcgat acctaaaact tttgctggag atgagtataa
     1381 aaatcaaaaa caaattctga ttgatgagca gcagaggaaa aatcgtcaaa taattcaaga
     1441 actggaaaag gaagccttag aagaagggtt tgccttccgt atgacttcta tgggtcccat
     1501 actgattcct ttggtagaag gaaaacccat gactcaagaa cagtacttag ccttaaccga
     1561 tggagaaaaa gaaattatag aaaatagaag gcaagaactt ctgacccgga tcagcgaggc
     1621 ttttgataaa attcaccagc tggaagtaga attgaaaaat aaaatagcag aacttgaccg
     1681 gagagtagcg gagtttgcca ttaatcctct tttcaaggaa ttagtggata aatacagttc
     1741 ctctcccgag gtgcttaagt ttttggagtc actgcggaat ttcactcttg cttatcttca
     1801 catcttcaga gactcttcag aagctcctca gcaacttcct cagcttcttc cttatcaaag
     1861 attgcaagat ccttttttac ctttcaagat aaatgttttt gtagacaatg cttctttaga
     1921 agaccctcca gtaattctgg aaacccatcc taactggacc aatcttttcg gaaagattga
     1981 gcgtcgagct tttatgggag cttatttcag tgaccatact atgttgaaat caggttctct
     2041 tcaccaggct aatggtggct atataataat gtatttccgg gatttaatca gtaacccggg
     2101 cgcctgggaa gggctgaaaa ggactttgaa aaatcgggaa atcaggttag aagacccttt
     2161 tgagcaattt ggtatcattg ctcctcaagg gttacgtcct gatcccctac ctttcaacgc
     2221 caaagttatt attatcggag atgaatacta ttatcgtgtt ttaactcttc tcgatgaaga
     2281 ttttcgagat ttattcaaaa taaaagctga ctttgactac cagatccaga gagaagaaag
     2341 gattctgcat tcctttgcct gctttgtcaa taattgttgt aaagaggaag ggcttttacc
     2401 ttttgaccgg acgggagtag caaaagtttt ggaatacgga gcacgggcag tttctcatca
     2461 aaaaaaactc agctctcaat ttggttttat gaaagatatc ctggtggaag ctgatttttg
     2521 ggcccgagag gaagccacag atcgagttta tgctcgccat gttcaaaagg cattacaaga
     2581 gagaaaaaag aggctgagcc ttatggctga ccgcctgcaa gaagctattt tagaagatgt
     2641 attgttaatt gatactcagg gtaaagtaac gggacaaatc aatgggttag cagtttatga
     2701 cctgggagat tatagttttg gccgaccttc ccgcatcact gcccgcactt atcttggaaa
     2761 acaaggagtg gttaacatcg agcgagagtc tcgtttgagt ggccgcatcc atgataaagg
     2821 agttctcatt ctggggggat atttaggcta taagtatgct cagaataaac cactttctgt
     2881 ggctgctagc gtttgttttg agcagtctta cgagggggta gaaggagaca gtgcttccct
     2941 ggccgaaacc tgcgctgtac tttccagtct ggcagaagta cctattcgcc aggatatcgc
     3001 cgttactggt tctattaacc agaaaggaga agtacaggct attggaggag taaatcaaaa
     3061 aattgaaggg tttttccgca ccgctcaaga gaaaggctta accggagaac agggagtaat
     3121 tattccccgg agcaacattc aaaaccttat gttagaagag gaagtagtag aggcagtgcg
     3181 aaaaggtaaa ttccacattt acacagtttc tactgtggac caggctatgg agatattaac
     3241 cggtcttcca gctggagaaa gaaaacctga tggaactttt gaagaaggaa cgctaaatta
     3301 cttggtggac gaaaaacttg ttaaaactgt ggaactattg aggagttttg aagaaaaaga
     3361 aggagagaaa aaagaggaaa atatctcaga gaataaggag gaatgattta cggaagaatt
     3421 ttctcgccgg ggactccggt ggttagttag aactgctgtt ctcttagctc ttactttagc
     3481 tatccagatg ctggggctgc ctcagttttt caccggccct ttagtgaatg cttttcttct
     3541 tttggctgct atattaaatg gaatatggag tggggtaata atagggttat ttactccctg
     3601 gatagctttt gtccgaggga ttcttgctcc cccattaggg ccaatgattc cctttattat
     3661 ggctggtaat gccatactgg taatagtttt cagcctcata gcaggaaaaa gagaaaactg
     3721 ggggagagaa gtaataggag ttattctggg ctctttccta aaatatctcc ttctctctca
     3781 agcggtaacg tgggtggtgc aagtacctcc tccggtagcc aaagctatgc agactcctca
     3841 gcttttcacc gctttagcag ggggagccat agctttaata atttaccggg taattcccat
     3901 caggttcaga agctataagt aaaagagtaa ggagtttttc tgcttttaga gaaaaagatg
     3961 tttattgctc cttattctct ttagcttctt tagatttttc tacaaaattg agctggagct
     4021 gggaaagctg agcttccagt tctcgtagct cactcccttc taaatctttt tttaccttct
     4081 ccaaaatcac ggaatagagg tcaatggact tacgcgcttc ttctaagtct accagcattt
     4141 ctccagtctc agggtgaact accagcccta aatattgcca ggctttagcc gaaagaagac
     4201 ccaaaaaata gaatcccaga tccttagttt tactgggttt cgcggttttt tcttcgctca
     4261 ttttgtatcc tcctccacta aatttctttc tataattgta taagcttctt ccaccagaat
     4321 agatagagat ctcccctctc cttctatcca ggttacagga gcttttttga accagttaat
     4381 ttgagttttc aaaaatcgaa aagtgccctg taatatttct tcttgagctt cttctctaga
     4441 aacatctccc cggaggagtt ttactatcgg gcgataagta aaattatcca gagccggaat
     4501 aggaggggaa tatccccggg aaaaaagctc ccccacttct tctactattc ctcttttaaa
     4561 catctcccct acccgatggt aaatcctttc ttttaattta tctctgtccc agcgaatacc
     4621 aatcaagaga ggcgaaaaag aagagggacg gggagaaaaa agagaaggag gatgaccggt
     4681 tttcttgaat atctcaagag ctctgatcaa tctttttcgg tcatactttc ctattttttg
     4741 cgctctccgg gggtctacat ttttcaaaag ggcataaagt ttttcagtag ataaacgctc
     4801 gagagcaatg cggatattat tttcagcagg agctcccaca aatagaaaag accgataaag
     4861 cgcctgaaga taaaaagccg tccctcctac cacgagaggg agatttcctc gctctctaat
     4921 ttctctctga agataaatgg cttcttttct aaactggaag gcactccacc tctccccggg
     4981 gtcaaggaaa tccacgagat gatgaggaac tctttctcga aaattcaccg ggggtttatc
     5041 tgtgcctata tccaggtatt tataaactgc taaggaatca gcataaatca actccacttt
     5101 tcctatccgc tctgccagag ctaaagatag atcagttttt ccggtagctg tagggccaac
     5161 gatagcggga agcttcctac tttttaactg aaccataaac tttattgccc tccccggggg
     5221 tcaaaactac ctctatccac tctcctacct ccatagagca gggttctctc aggtataccc
     5281 gcaggttatt agcccctcta cctgataaca gccctccttc ttttcggtca accaatattt
     5341 ccattacttt tccctgcagg gaaggtaatt tttctcgata gatgtttcgc aaagtgtcgt
     5401 tcaaaacatg gagacgttcc tttttttcgg ggagaggaac atcgtcttct aaaagataag
     5461 ctgcagtccc tggccgggga gaataaacat aggtataagc tacgtcaaat tgtgcttctc
     5521 taactacatc cagagtctcc tggaaatcag aatctgtctc tccagggaaa cccacaatga
     5581 tatctgtccc gattctcgcc gagggaatag actgacggat gaggttaact ttattcaaat
     5641 aatcctcccg ggtataaccc cgcttcataa gctgtaaaat cttattagaa ccagcttgaa
     5701 gaggaagatg aaaataaggg caaagcacac ggctatcttt tactattctt actattttct
     5761 ccctgaagtc tcgagggtga gaggtgagaa aacgaaccca gattttttta ttcccgagcc
     5821 actcctcgat atctagaagc agctcttcaa aagcttcttc tttcctatcc cgaccgtaac
     5881 tattgacatt ttgcccgagg agggtaactt ctcgaaagcc ttcttctgcc aggttcatta
     5941 actcttcttt tattatttct ctaggcttac ttctctcttt cccagtagta taaggaacca
     6001 cacaataact acagaagtta tcgcaaccaa aagtaatagg taaataagca gaaattttac
     6061 tggaatgaca ataccctctt tcttcataaa gagaaggagc ctcttcctgg aagtctacta
     6121 tctttacttt ttcctgccag gcctttttaa ttaaaaaggg tattttcccc caattatgag
     6181 taccagctaa aacatctatt ttttcatctt ttccgaggag tttttctctc tctaactgtc
     6241 ctaggcagcc acatactaca attatagaat ccgggttttt ctcccttctc tcttgagcaa
     6301 atttaactat aatattcatc gcccgttctg cggcatgttc tcgcactgca caggtattaa
     6361 acacaaaaat atctgcttcc tctgggcctt cggccagata aaatcccagc tcttgcagtt
     6421 gataagccag atgctcggaa cggcttttat tcatctgaca accaaaagtt tccacccaaa
     6481 aagtagctaa agctacttcc ctggccgtaa aacctggttt tcttttaatc tccactacat
     6541 ctctcgaaac gcttaagcgt ttcgaggctt caccaccagt ttaatagctg tcctctcctc
     6601 cccatcaatg ttgatatcag tgaaagccgg catacaaacg agatcgatgc cactaggagc
     6661 aacataccca cgagctatag caatagcttt aatagcctgg ttgactgctc ctgctcctac
     6721 cgcctgcact tcagctatac ccttttctcg gatagctcca gctaaagcgc cagctacagc
     6781 attgggatta gacttagaag atacccttag aacatccatg gccattcctc cttcgttttt
     6841 tttactgatt ttaagatatg gacaagcgag agatttccgt agctcttccc gtaatctcat
     6901 ctacctccac tacaataccg ttaagtttaa cattatcttt agctacttga tatctgatag
     6961 gcatttgagt tagaaatctg cttaaaatgt cttctgtttt cactccgata actgaatcat
     7021 gagctcctgt cataccgatg tccgtaatat aggctgtgcc ttgaggaaga attcgctcat
     7081 cagcagtagc cacatgagta tgagtgccaa aaacacaaga taccttacca tccagaaacc
     7141 atcccatagc tatcttctct gaagaagcct cagcatgaaa gtcaagcaaa attaaattgg
     7201 ttaccttctt aaagttctcc agttcttctt ctattcgatg aaacgggcaa tccagagaag
     7261 gcataaacac ccggccactc atattagcca ctatccactt tttacccttt ctttctacca
     7321 ttacccaacc cttacccgga actcccggag ggtaattgag gggacggata agccggggct
     7381 gataatcgat ataatccacg atttctttct tatcccagat gtggttacca ctcgtaatta
     7441 catctactcc ataactcaaa atctcctcgc acacttctgg cacaatcccc aaacctccgg
     7501 cagcattttc tccgttgacc accactccct caatatctaa ttcctcttta atagttttta
     7561 atttatcagc cagtatttgc cttcctggct ttcccactac gtctcctact atcaaaaacc
     7621 gcaaggattt atccctccgt tatttagcat attctacaga cctggtctct ctaattaccg
     7681 ttattttaat ctgcccggga tactccagtt ctttttctat tttacgagct atatcataag
     7741 ccagctttgt cgctcgggca tcatcaattc tatcgggctt tactataatt ctaatttctc
     7801 tccccgcctg aatggcataa gatttctcta ctccctcaaa atcatcggca attttctcca
     7861 atttctccag acgtttgata tatgactcca agctttctct tctcgcccca ggccgagaag
     7921 ccgatatggc atcagctgcc tgcaccagaa ctgcttccac ag
//
LOCUS       DAOSVE010000080         7959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_4633_length_7959_cov_66.972293, whole genome shotgun sequence.
ACCESSION   DAOSVE010000080 DAOSVE010000000
VERSION     DAOSVE010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4633_length_7959_cov_66.972293"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PK016_06895"
     CDS             complement(<1..485)
                     /locus_tag="PK016_06895"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HXL02821.1"
                     /translation="MYRTNLLKPVTEKDISELFSRLEKIIVSESNTQQRLEKVIRKQI
                     MEEFISFPNHEASSLPDYITKAIEIIQKRYQEKITLREITEEVNATPAYLSYSFRKYT
                     GQTIVEYINKLRIEVSKCLLINSALQIQEVAEKVGYEDAKYFSRVFHGLVGCSPTTYR
                     R"
     gene            complement(472..792)
                     /locus_tag="PK016_06900"
     CDS             complement(472..792)
                     /locus_tag="PK016_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXL02822.1"
                     /translation="MKEGLFLQIILVDDEDFFLNYLEFLIQGVSEAYQVVAKASSGLE
                     ALAILEMVYPDLIITDIKMPEMDGLTMLKCAKERGWRGKSIVVSGYDDFTLVQEALRS
                     DVQD"
     gene            complement(1160..2107)
                     /locus_tag="PK016_06905"
     CDS             complement(1160..2107)
                     /locus_tag="PK016_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HXL02823.1"
                     /translation="MEDIDMVVKAAELYYISDLSQREIAEIMGISPQKVFRILKKAKE
                     LGLVEIKIRKIGEVDKGLSLSLKEKFSLKDAIVAETYSNVPEQVIKAVAQAAANFLKS
                     LLESSSYSSVGVAYGRTLGEVLEYLPFFNFSNLKVVQMMGSYGDRRWRTLAIELVRSF
                     SKHLGGEPLYLFVPAFAKDSLSKEVFLQDRETREILEMAENVDLALVGIGGIKRETSL
                     LIETGGFHEEEIQELIDRRAVGAICGSFFDIEGKIVHSTIDSRRIAVDLERLRKNAKR
                     IVGMAGGLEKRETILGALRGKWITDLVTDYEVAKWLVTQ"
     gene            complement(2174..3085)
                     /locus_tag="PK016_06910"
     CDS             complement(2174..3085)
                     /locus_tag="PK016_06910"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HXL02824.1"
                     /translation="MDYIEYKDKLSGTFAPMVVPFNEKGEIEFGALKRNLEKMSQTSL
                     KGYFVLGTNGEFRSLSYEEKVEIVRVTLSLKKDKIVIAGASCESTMESIKLAKELSNL
                     GVDFISLMPPSFFAKKMTDDILFGYFSEVADNIDKPVLLYNNPGVANNLCLSPTLISR
                     VSKHPNILGIKDSSKGNYDSYISSSLGENFWVLAGSADFAFPALLLGAVGGVLSLANV
                     FPELCNNLFRLGMERNLESGLTFHRKIMKLNSLVSGKYGVSGVKAAMDIFGFEGMYPR
                     KPLLGLSSVQREELEREVNKVLVELKQ"
     gene            complement(3105..4028)
                     /locus_tag="PK016_06915"
     CDS             complement(3105..4028)
                     /locus_tag="PK016_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004066788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HXL02825.1"
                     /translation="MKKWKVLLPQPIAKAGMELLEKSDKFEVIVFSSPDMEEVKRVAG
                     EIDAIVVRSGFKVHKELIDLAPRLKVISRVGAGLDNIDVAYAQSKGIKVLNVEGINAL
                     SVAEHTISFILALAKNLFEFDRCVRQGSWDIRYAYKAFELEGKILGLVGLGSIGKEVA
                     RLALALGIKVVFYDPFIKEIGGLSVERIDDLQVLLRQSDFVSLHVPLNEDTKKMIDED
                     ELRTMKPTAFLINVSRGLIVNNEALCKALQEKWIAGAGIDVFAEEPPPLDDPLLRLEN
                     VILTPHVAGLTKESSEKVALQAIENLIKALE"
     gene            complement(4049..5593)
                     /gene="xylB"
                     /locus_tag="PK016_06920"
     CDS             complement(4049..5593)
                     /gene="xylB"
                     /locus_tag="PK016_06920"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HXL02826.1"
                     /translation="MNSKKPCVVGVDVGTSSCKTLAIDENGKVLASGVEEYPVYTPLP
                     GWSEQNPDDWWKAVRTTLKEVSGKVIEKGYMVEGIGLTGQMHGLVLLDAEGNVLRPCI
                     MWNDQRSAPYCSQIYQRVGGESEFLKIANNVMLPGYTGGKILWVQDREPQIFEKASKF
                     LCPKDFIRFKLTDDFATDVTDASGTGLFDVRERKWATDLIQELGIPLSMFPASFESHE
                     KTGVIKEEVANAIGIQSGTPVFAGGGDAVVQTIGIGAVMPSILSITIGTAGIVAVSSD
                     KFLKNPGGTLQFFCNAIPDGWIVFGTTLAAGGSLRWFRDIFGGVEMQAAQWLKKSVYD
                     LLGEEGELSPPLARGILFLPYLIGERCPHSDPDARGVFVGFGLHHSRNDIVRSVFEGI
                     VFSLRDVYQHIEQLGFDISQIRLSGGGAKGKLFQKLHADIFGSEVVTVRYGEEGGSYG
                     AAILAGVGAGFWPSVKDAAQCIEIETRVEATSEYVEIYRDFFKVYQSLYGTLKPTFEI
                     LSRFQS"
     gene            complement(5627..7066)
                     /locus_tag="PK016_06925"
     CDS             complement(5627..7066)
                     /locus_tag="PK016_06925"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HXL02827.1"
                     /translation="MLRQLMIIGGKLAESSEGQYIEVINPATEEKIGEVPVASFNQID
                     EAVNRAAEAFQEWSTKSPFERAKFMEKAALEIEKKAKDIGKVLTMEEGKPLKQAVSEV
                     QGTAEALRYFAEAGKRAFGEIIPLEKSNLSSLVVKQPIGVVAAITPWNYPVQLLSWKV
                     GAALAGGCTVVAKVPSETPFSPLMFLQCFLDAGFPKGVVNGIAGSGSTVGKNLVLHPL
                     IRKVSFTGSTEVGKEIMHYCADGVKRVALELGGQSPLIVLQDADLEKAVKGGVRRSFR
                     NMGQVCNSINRIYVERPIFERFVEMFVEETKKLTIGNGLIEDVDLGPMVSLNAWQRAK
                     EHIDDAVAKGARILTGGGKPVGEKYKKGYYLEPTVIVDVNHQMLLMSEETFGPVVGIM
                     PFDKIEEAITWANSTRYGLAAYLYTSSLDHARQISLGLECGNIGLNNVDVATIYAPYT
                     GWKESGFGSDLGPEGVSSYLEEKHIKVEF"
     gene            complement(7094..7468)
                     /locus_tag="PK016_06930"
     CDS             complement(7094..7468)
                     /locus_tag="PK016_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047869 - dimethylpropiothetin
                     dethiomethylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfonioproprionate lyase family
                     protein"
                     /protein_id="HXL02828.1"
                     /translation="MVFKTNIHEMQPDTCERSFRYLAQNKKGETLRDTYWLIDPEASP
                     AKVLKLGYTVVYPEGKTTGHSHAEEEVYFVLSGRGEMEIENERFPIQEGDAFYVEPGK
                     HHVTYNTGVLPLTIIWTTGKIE"
     gene            complement(7487..>7959)
                     /locus_tag="PK016_06935"
     CDS             complement(7487..>7959)
                     /locus_tag="PK016_06935"
                     /inference="COORDINATES: protein motif:HMM:NF019998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HXL02829.1"
                     /translation="INEGELQQIGTVEEIWNHPANKFVAYFVGEPTMSFLPGKVESPW
                     TIVIPCSQGERVFSLESPVDQKYAGKMVTIGVRPQQIEISREKNRENTIQGIVKIIEF
                     QGDKTILTVKLEDEPESEVKVVVSAQEKYSEGEVVWLYFAPQIIHLFIDDVSIL"
BASE COUNT         2410 a         1972 c         1148 g         2429 t
ORIGIN      
        1 tttcttcgat atgtggttgg agaacaacct accagcccgt gaaaaacacg ggagaagtat
       61 ttggcatctt catatccaac cttctccgct acttcttgaa tctgtaaagc actatttatc
      121 aaaagacatt tggaaacttc gattctcaat ttatttatgt actctactat cgtttgccca
      181 gtgtatttac ggaaactgta actaagataa gcaggggttg cattgacttc ctcagttatt
      241 tcacgaagag tgattttttc ttgatatctc ttctgaatta tctctatagc tttagttata
      301 tagtccggta acgaagaagc ctcatggttt ggaaaagaaa taaattcttc cataatttgc
      361 ttacggatta ctttttcaag acgttgctgt gtattactct cagaaacaat aattttctca
      421 agcctcgaaa aaagttcaga aatatccttt tcagtgactg gcttaagtag attagtcctg
      481 tacatcggag cgtaaagcct cctgcaccag agtaaaatca tcatacccac taaccacaat
      541 acttttcccc ctccaccctc tttcttttgc acatttcaac atggtaagtc catccatctc
      601 tggcatttta atatcagtga ttatcaggtc cggatagacc atctctaaaa tcgccagggc
      661 ttctaagcca ctcgacgctt tagcaactac ttgataagcc tccgaaacac cctgaatcaa
      721 gaattcaagg taattcaaga agaaatcttc atcatccacc aatattattt gcagaaacaa
      781 accttctttc atcaccaatc cctcctattt cccggataag ctaacttttt ataactaact
      841 tcctcccctt taaatcatca taaaaggtag caacaaatta gaaaacgcta tgccttcata
      901 tgatttcacc accagtttta agttttctcc cggcactctt tttcaattgt gagacataca
      961 tccatctcct atcctccata ttagcttttg aggctttctt cattcaatta taatggaatc
     1021 tgaaaagtag acagagtaat aagatggcct gtaaacgaaa agaagaaaga tattaccggg
     1081 atttataaca tagagagaat gtttccacac aagttggcat agagccgcta aaaacttgac
     1141 gagctcttac atgattacat cattgagtaa ctaaccactt tgcaacttca tagtcggtaa
     1201 ccaaatcagt aatccattta cctctcagag ctcccaaaat ggtttccctt ttttccagac
     1261 ctccagccat tcccacaatt cttttagcgt tcttcctcaa cctttcgagg tctaccgcaa
     1321 tcctccttga atctatagtg ctatgcacta ttttaccttc tatatcaaaa aaactaccac
     1381 aaattgctcc tactgccctt ctgtctatta gctcctgaat ttcctcctca tggaaaccac
     1441 ccgtctcgat tagcaagctc gtttctctct ttatacctcc tatacctacc aaggctaagt
     1501 ctacattttc tgccatctcc agaatttctc gagtctctct atcctgtaga aaaacctcct
     1561 tactcaaaga gtccttagca aaagcaggaa caaataagta gagcggttcg ccccccaaat
     1621 gtttactaaa agaccttact aattcaatag ccagcgttct ccatctccta tcaccgtagc
     1681 tacccatcat ttgaaccact ttcaaatttg aaaaattaaa aaatggtaaa tactcaagaa
     1741 cctccccaag cgtcctgcca taagctacac caacgcttga gtatgaagaa gattctaaaa
     1801 gagattttag aaaattggct gcagcttgag ctactgcttt aataacctgt tcaggaacat
     1861 tactataagt ctcggctact atggcatcct ttaagctaaa tttctctttc aaacttaaag
     1921 ataacccttt atctacttct ccaatttttc taatttttat ttctactaaa cccaattctt
     1981 tagctttttt caaaattcta aaaactttct gaggagaaat acccataatt tcggcaattt
     2041 ctctctggct taaatcagaa atatagtata attcagctgc cttaaccacc atatcaatat
     2101 cttccaaagg tcctcttctt tgaaccaaag ctccctatca cccctttcag caaatgtctt
     2161 aaaagcacaa agactattgc tttaattcta ccagaacttt attaacttct ctctccaatt
     2221 cttctctttg gaccgagctt agccccaaaa ggggtttccg aggatacata ccttcaaagc
     2281 caaatatatc catcgctgct tttactccac ttaccccata ttttcccgaa actaaggaat
     2341 taagcttcat aattttacga tgaaaagtca aacccgattc taaatttctc tccataccta
     2401 agcgaaatag attattgcat aactcaggaa aaacattagc caaagaaagt acccctccta
     2461 ctgctcctaa aagtaaagcg ggaaatgcaa aatctgctga accagctaaa acccaaaaat
     2521 tttctcctaa agaagaagaa atataagaat cataatttcc tttagaagaa tcttttatac
     2581 ccaaaatatt aggatgcttg cttacccttg aaatcaaagt cggagataaa catagattgt
     2641 tagccacacc agggttattg tagaggagaa ccggcttatc tatgttgtcc gctacttcag
     2701 aaaaataacc aaataagata tcatctgtca tttttttagc aaagaaggaa gggggcataa
     2761 ggcttataaa atcaaccccc aaattagaaa gttctttagc aagttttatt gattccattg
     2821 tagattcaca acttgcacca gcgatgacaa ttttatcttt cttcaaggat aaagttaccc
     2881 taactatctc gactttctct tcataactca aacttctaaa ttccccgttt gtacctaaga
     2941 caaagtaacc ttttagagaa gtctggctca tcttttccaa attcctcttt aaggctccaa
     3001 actcaatttc tcctttctca ttaaaaggta ctaccatagg agcaaatgtt ccagataatt
     3061 tatctttgta ctctatataa tccattctca taacctcctt gattttattc cagagctttt
     3121 attaaattct ctattgcttg aagagcaact ttttcactac tctctttagt tagaccggct
     3181 acgtgaggag ttaaaataac attttccaaa cgaaggagag gatcatctaa agggggaggc
     3241 tcctcagcaa acacatctat accagctcca gctatccatt tttcttgcaa agctttacaa
     3301 agagcttcat tattcacaat caaacccctt gaaacattga ttaaaaaagc agttggcttc
     3361 atcgttctaa gctcatcctc atcaatcatc tttttagtat cttcgtttaa aggtacatgt
     3421 agagaaacaa aatcactttg ccttagtaag acttgaaggt catcaattct ttccactgaa
     3481 agtccaccaa tttctttgat aaaagggtca taaaatacaa cctttatgcc taaagcaagt
     3541 gcaagacgag caacttcttt cccaatagag ccaagaccca ctaatcccag tattttccct
     3601 tcaagctcaa aagctttgta agcgtaacgg atatcccaac ttccttgacg tacacaacga
     3661 tcaaactcaa aaagattttt cgctagcgcc aggataaaag aaatagtatg ttcagcaact
     3721 gaaagcgcgt ttattccctc cacatttagt actttaatac ccttactttg agcataagct
     3781 acatcaatat tatccaatcc tgcacctacg cgagaaataa cttttaacct gggagccagg
     3841 tctataagct ccttatgcac tttaaaacca cttctgacaa ctattgcatc tatttcccct
     3901 gcgactcttt taacttcttc catatcagga gaagaaaaaa ctatgacttc aaatttatca
     3961 cttttttcca aaagctccat accagcttta gcgattggct gagggagcaa gaccttccac
     4021 tttttcatcg tttatttttg accccctctc aactctgaaa acggcttaat atttcaaatg
     4081 ttggttttag agtaccatat aaggactgat atactttaaa aaaatcccgg tatatttcta
     4141 catattctga agtagcttca actcttgtct caatctcgat acattgagca gcatctttta
     4201 ccgagggcca aaaacccgct cctacaccgg cgagtatagc tgctccatag ctacctcctt
     4261 cttctccata tctcactgtg accacctcgg aaccaaaaat atcagcatga agtttttgga
     4321 ataatttgcc ctttgctcct cctcccgaaa gtcgaatttg ggaaatgtca aaacctaatt
     4381 gctctatatg ttgatataca tcacgcagac tgaaaacaat gccctcaaaa acacttcgta
     4441 ctatgtcatt acgagaatga tgcaaaccaa aaccaacaaa cacacctcga gcatcaggat
     4501 cgctatgagg acatctctcc cctataagat aaggcaaaaa gagaatgccc cgggctaaag
     4561 gaggagaaag ttccccttct tcaccaagta agtcataaac cgacttcttt agccattgag
     4621 cagcctgcat ttctaccccg ccaaagatgt ccctaaacca tcttagagaa ccaccagcag
     4681 ctaatgttgt tccaaaaact atccaaccat caggaatggc attacaaaaa aactgtaaag
     4741 ttcccccagg gttttttaga aacttatctg aagatacagc tactatcccc gcagttccta
     4801 tggtaatact cagtatactg ggcattaccg ctcctattcc gatagtttgg acaactgcat
     4861 cacctccacc ggcaaaaacc ggtgttccac tctgtattcc aattgcattt gcaacttcct
     4921 ctttaataac tccagttttt tcatgagatt caaaagaagc aggaaacata gaaagaggga
     4981 ttcccagctc ttgaattaaa tcagttgccc atttcctttc cctcacatca aacaaacctg
     5041 tgcctgaagc atctgttacg tctgtagcaa agtcatccgt taacttaaat ctgataaaat
     5101 ctttaggaca taaaaatttg ctagcttttt cgaaaatttg aggctcacga tcttgaaccc
     5161 ataaaatttt tcctccagta tacccaggca acatcacatt gttagctatt ttaagaaatt
     5221 ccgactctcc acccactctc tggtaaatct gcgaacaata tggagcgctt ctctgatcat
     5281 tccacatgat acagggtctt aaaacatttc cttctgcatc caaaagaact aaaccatgca
     5341 tctgaccagt taaacctatc ccctcaacca tataaccctt ctctattacc ttacctgaaa
     5401 cctcttttaa agtcgttcta actgctttcc accagtcatc cgggttctgc tctgaccagc
     5461 ctggtaaagg ggtatacacg ggatactcct ctactcctga agcgagcact ttaccatttt
     5521 catctatagc caacgtttta caacttgaag ttccaacgtc cacacccacc acacaaggtt
     5581 ttttgctatt catattctca ctctccacac gcgctcagcc cacagattag aattccactt
     5641 tgatgtgttt ttcttctaaa taggaagaaa ctccttctgg ccccaaatcg cttccaaagc
     5701 cactttcttt ccatccagtg taaggagcat aaatcgtagc tacatctaca ttattgagtc
     5761 ctatattacc acactctaac cctaaagaaa tttggcgggc atggtcaaga gaggaggtat
     5821 ataaataagc tgctaggcca tatcgggtgc tattggccca agtgatagct tcttcaattt
     5881 tatcaaaagg catgatgcca actaccgggc caaaagtttc ttcgctcatc agcaacatct
     5941 gatgattcac atctacaatt acagttggtt ctagataata acccttttta tacttttcac
     6001 ctactggctt tccacctcca gtaagtatcc tggcaccttt cgccaccgca tcatctatat
     6061 gttcctttgc ccgctgccag gcgttaaggc taaccatagg gcctaagtca acatcttcta
     6121 tcaaaccatt accaatagtg agtttttttg tctcttccac aaacatttcc acaaatcttt
     6181 cgaaaatcgg tcgctcaaca tatatccgat taatagagtt acaaacttgc cccatgttac
     6241 gaaaagacct tctcactcca cccttcactg ctttttccaa atcagcatcc tgaagaacaa
     6301 taagcggaga ctgcccacct aattctagcg caactctttt tactccatcg gcacaatagt
     6361 gcataatttc tttgcccact tcagtagaac cagtaaagct aacttttcta attaaagggt
     6421 gtaaaactaa atttttaccc acagtagagc ctgaaccagc aattccatta accactcctt
     6481 ttgggaaacc agcatccaga aaacactgca ggaacattaa gggagaaaat ggcgtttcag
     6541 aaggaacttt agccacaacc gtacatcctc cggctaatgc tgcaccaact ttccaagaaa
     6601 gaagttgtac aggataattc caaggagtaa tagctgctac aactccaatt ggttgtttta
     6661 ccaccaaact actcaaatta ctcttctcaa gaggaattat ctctccaaaa gctcttttgc
     6721 ctgcttcagc aaaatatcgt aaagcttcag ctgtcccttg cacctcgctt actgcttgtt
     6781 tgagaggttt accctcttcc attgttaata ctttccctat gtccttcgcc ttcttctcta
     6841 tttctaacgc tgctttttcc ataaactttg ctcgttcaaa aggactttta gtagaccatt
     6901 cttgaaatgc ctctgctgct ctatttactg cttcatcaat ttggttgaat gaagcaactg
     6961 gcacttcccc tattttctct tccgtagctg gatttattac ttctatatac tgtccctctg
     7021 aagattcagc caatttccca ccaataatca tcaattgcct taacatggta attttcctcc
     7081 ctatcttcta tcgctattct atttttcccg tcgtccagat gatggttaaa ggtaaaacac
     7141 ctgtattgta agtcacatgg tgctttcctg gttcaacata aaaggcatct ccctcttgga
     7201 taggaaatcg ctcattttca atttccatct cacctcttcc gcttaagaca aaatatactt
     7261 cctcttcagc atgagaatga ccagtggttt ttccctcagg ataaaccact gtatatccca
     7321 actttaacac tttcgctgga gaagcctcag gatcaataag ccagtatgtg tcacgaagag
     7381 tttcaccctt cttattttgg gcaagatacc gaaaagaacg ttcacaagtg tcaggctgca
     7441 tttcatgaat attagttttg aaaaccattt tcgatagaac acctccctat aaaattgaaa
     7501 catcatcaat aaaaagatga atgatttgag gagcaaagta aagccatact acctctccct
     7561 cactatattt ctcctgagca gaaacaacaa cttttacttc actttctggc tcatcttcaa
     7621 gcttcacagt caatattgtt ttatctcctt gaaactcaat aatctttaca ataccctgga
     7681 tagtgttttc ccggtttttc tctcgagaga tttctatttg ctgaggacga acacctatag
     7741 taaccatttt accagcgtat ttttgatcca ccggggattc taaagaaaac accctttccc
     7801 cctgggagca tggaatgact atagtccaag gagattcaac ttttcccggc aagaaactca
     7861 tggttggttc tcctacaaag taagctacaa acttattggc tggatgattc caaatctcct
     7921 ctactgtgcc aatctgctgt aactctcctt cattaataa
//
LOCUS       DAOSVE010000081         7917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_4651_length_7917_cov_68.030018, whole genome shotgun sequence.
ACCESSION   DAOSVE010000081 DAOSVE010000000
VERSION     DAOSVE010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 7917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7917
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4651_length_7917_cov_68.030018"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PK016_06940"
     CDS             complement(<1..232)
                     /locus_tag="PK016_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02830.1"
                     /translation="MYKKILVLSLTFLFLLSGCNWFSDNPMFPRAKIIIAGTPIEEDT
                     GKIAFEFVAVNKVGAQLTECHLKYKKPDGTELT"
     gene            complement(1043..3010)
                     /locus_tag="PK016_06945"
     CDS             complement(1043..3010)
                     /locus_tag="PK016_06945"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HXL02831.1"
                     /translation="MNHKEVDIPNTDKKTSLPFFRVYQLGSEKTVEFAADELKKYLQR
                     ILGKDTSVVLEKKESFNQLEKEAIWVGTSEKFYPGLCSETEEQKLDDAIAIEIKSGSG
                     VITGINPRSVLLGVYRFLTEIGCRWVRPGIDGEILPSRESPDFTVQIKEKAAYRHRGI
                     CIEGAVSLENVRQIIDWAPKVGFNAYFIQFREAYTFFERWHTHRHNPFKQPEPFSLDT
                     AREYTRELEKEIKKRGLLYHAVGHGWTCEPLGIPGLSWEPGDYHVSPEYAGYLAKVDG
                     KRTIWEGIPLNTNLCYSQPEVRKLIVNEVVTYLQKHPEVDFLHLWLADGSNNNCECDD
                     CQRALPSDFYLQILNEVDEALNKQGLTNKIVFLIYVDLLWPPKETRLKNEDRFVMMFA
                     PITRTYQCPYPSTMPSLPLVPYNRNHLQFPTTVEGNLAFLREWQKIFSGDSFAFEYYL
                     MWAHYADPGDYKIAWLISEDIKNLKNLGLNGLISCQVQRAFFPTGLPLYLMGKLLWND
                     NLSFEEIVEDYFRSAFGPEGKKCYEYLKNLSQLFTPLFQEENSKKEEIYECGEKIEKL
                     IKEFRPIIEKNAQRDCISEAQSWRYLEYHAEICSQLAKILVEKQKGDKEKSREWWEKL
                     KTFLQKNEDQMQPVFDLFEYIETMEGRILSK"
     gene            complement(3041..3886)
                     /locus_tag="PK016_06950"
     CDS             complement(3041..3886)
                     /locus_tag="PK016_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXL02832.1"
                     /translation="MERSLREEIGPLPRIVLYVLLLGFTFLTLYPLIWMAINSFKTTQ
                     EFLLNPLGLPNRWFAKNYSLAWRLANFSTLFLNSILYTGISTIGVIWFSLAAAFAFAK
                     IKSRFTPFLYGGFLIGILFTIESLMIPLYLMAFTTGLVNTRVGVLIPYIGLGLPLGIY
                     LCTEFIKGLPDSVIEAGRIDGASYLKIFIYIIIPMAKPVIATVAILNGLGIWNEFMLI
                     NILVSRGNLLSLPVGIMKFSGALASDYGKQFAALVIGTVPTVTFYLVLRNRITQGVAV
                     GATKG"
     gene            complement(3937..4851)
                     /locus_tag="PK016_06955"
     CDS             complement(3937..4851)
                     /locus_tag="PK016_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXL02833.1"
                     /translation="MVNPPHLYSKSEERRAYWLLVLPAVVIYWAVTAFPLLFSIVLSM
                     TDYSGGPIFAFDFKFVGLHNYVSMFKDPYFWISLKNNVYIILISVLGQIPLGFIFAYV
                     IFRKLVRFGDFFQTMIYLPAVISPIVVGILWQTFFSPYGPLTKIMQHFQPGWRSTFLL
                     DPKRAIIPVLFVMLWMYTGTYLIIFLANLQKIEPTILEAAKIEGATEFQTLWHVILPA
                     LSGVIVTTIILAISGSLKSFNLIFAMTQGNPARRTSVLALYMYDTAFRGAPNYPKASA
                     ISIFIVILSIILIIITKAVENRYGGREE"
     gene            complement(4968..6284)
                     /locus_tag="PK016_06960"
     CDS             complement(4968..6284)
                     /locus_tag="PK016_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXL02834.1"
                     /translation="MYIKKSRVISIVLAVFLITAGISGLSAQEQITLKVLNYLDLTSP
                     GTEREIKEIWEAFEKNNPDIKIEREDLFGEPFHQKLEAYAAAGNLPDVIYMWPGARSA
                     TLHDKKLVKDLRPFLGDEINDFVPAAVDPNNQRGKYLAMIPIGITATHMLYVNQEMLN
                     EWGVSVPQTYDELKNMVSKAKEAGKSLVVMGAQDDWVIQSVLFSMLVGRLLGNDYVDK
                     LVAGEAKFTDEPFVSVLKFYQQLFDDGVLTPDIVMTPYGTSPALFATGEAPLTIDGDW
                     RVGAFLTDPTTGEALIPPEKQNNILLTIFPAIPGEVTPNSTSMVLATGFGMSAAIPDG
                     SPKEEAAWRLIKWLVSPEAQKIRLETGAAIPSRKGVTAEGLEPLVQQRLGFYEKYVGT
                     YVLDDVLDPKIYLPINTGLQEIALGMSTPEDVASKVQKGYEEWRAE"
     gene            complement(6463..7641)
                     /locus_tag="PK016_06965"
     CDS             complement(6463..7641)
                     /locus_tag="PK016_06965"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HXL02835.1"
                     /translation="MPKSQKSLNSTRVVRLYNVTSLLRTLYREGGCSQTRLAKLTHMS
                     PATVSRITSELVDQKIIIEKGTGKSSAGRKPTIFQLNYEKIYIAGACISRDQVGLAIA
                     DLKGEIVSKRYFQPYSLEPESLIKELVHEFDLLVQETSINRKYILGLGLAISGIVDSE
                     QKTLVRSVNLGWREVKIGEILEKELGLPVLVENDANAAALAEFWFGGATEARSFFYIK
                     TDTGVGSGIIFERNLLAGPRGTAGEIGHLCFIPGGRKCRCGLHGCLETYLYAPDIKRR
                     YEEETGNRLESISHLKEKVQEGDPVARKIIDEAAEALSIAVLFAAGLLDLDLVVVGGA
                     WGALGDGFLNLIREKTMDRWERSGIGRIVPVTGSCLGESSDLLGAVGLVIHRWLASPI
                     "
BASE COUNT         2332 a         1676 c         1561 g         2348 t
ORIGIN      
        1 cggttagctc agtgccatcg ggttttttat atttgagatg gcactcagtt agttgtgctc
       61 caactttatt taccgcaaca aactcaaatg ctatttttcc tgtatcttct tcgataggag
      121 tgccagcaat aattatcttt gccctgggaa acatggggtt atcagaaaac cagttacaac
      181 cagataacaa aaataagaaa gttagactga gaactaaaat ctttttatac atttaccctc
      241 ctcctcacta aagttataaa tttgttctga aaatgttcta ttggatttac tgaagtcgca
      301 aaatctcttt aaaagagcta ataatagagt ttatcttaga atgtaaaata actctctaaa
      361 atttcttact tatagtagta aaataatagt gcaaaatcaa gtactttcgt taaattatct
      421 caaggcggtt cactcggatg tttttctgcc tttttagtgc ttggaatcaa atacacagct
      481 ttcatttata taccttaaaa attgtagcta ttttttgtag tatcctgcaa gactaccatg
      541 aatgtaaggg aggaagcatt ctctcttatc agccgccttc ttttttgtta ctccgacgat
      601 ctttaaactg tttttagcct gcccctgaat ggttcagaac tataagggga agaccccttt
      661 ataatctccc aaaaagacta aagagataaa taaaaaacat aaaaaaagca aaaactacta
      721 aggaaggaaa acagacagcc tacggcgtaa gattaaatga ctaaaagaaa aacagaaaat
      781 acaaaagact actgagggag aaaaagcctc cctcagtgga ttcccgatag aaaatctcag
      841 gaatgacgag aatgaaaaac tctccaggag gaagaaagag atggaaaaag aagaaagctc
      901 tccaagctcg taataacctc ctgagagaga ttagctgcaa taactcctta gtatgaaaga
      961 gaatagacgc aaagaaaaag aacaccgcaa tggattttgg gaacaggctt cttatcaaag
     1021 ttgggaagaa aagtttgcca ccctattttg ataatatcct tccctccatg gtctcaatat
     1081 attcaaaaag gtcgaatacc ggctgcatct gatcttcgtt tttctggaga aatgttttaa
     1141 gcttctccca ccactctctg cttttttctt tatctccttt ttgcttttct acgaggattt
     1201 tagctagctg agaacaaatc tcagcatggt attctaaata tcgccacgat tgagcttcgc
     1261 ttatgcagtc tctttgagca tttttttcaa taatggggcg gaattctttg atgagctttt
     1321 caatcttttc tccacattca tatatctctt ctttcttgga gttctcttct tgaaatagag
     1381 gagtaaaaag ctgagagaga tttttaaggt actcataaca ctttttaccc tcaggaccaa
     1441 aagcactccg gaaatagtct tctactatct cctcaaagct gagattatcg ttccacaata
     1501 actttcccat aaggtataag ggaaggccag tagggaaaaa ggctctttgc acctgacagc
     1561 taatcaggcc atttagtcct aaattcttca gattttttat atcctcgctg attagccagg
     1621 cgattttata atctcccggg tcggcataat gtgcccacat aaggtagtac tcaaaggcaa
     1681 agctgtctcc agagaaaatt ttctgccact ctcgtagaaa agcgagattc ccctctacgg
     1741 tagtgggaaa ttgcaagtga ttccgattat aaggcacaag aggtaaagaa ggcatggtag
     1801 aaggataggg gcattggtaa gtcctggtga taggggcaaa catcattaca aaccggtctt
     1861 cattttttaa acgagtctct ttgggcggcc acagaaggtc aacatatatg agaaacacta
     1921 ttttattagt taatccctgt ttgttgagag cttcgtccac ttcgttgaga atctggagat
     1981 aaaaatcgga ggggagagct ctttgacagt catcgcattc acaattgttg ttagacccat
     2041 cagccagcca caaatggagg aaatcaactt ctggatgctt ttgaagatag gtgacaactt
     2101 catttactat caatttcctg acttctggct gagagtaaca gagattggtg ttgaggggaa
     2161 ttccctccca tatggttcgt ttcccatcca ctttggctag ataaccagca tattctggag
     2221 aaacatgata atcgcctggc tcccagctca atccaggtat acccagcggt tcgcaagtcc
     2281 agccatgtcc tacagcatgg tagagaagac ctctcttttt tatttctttt tccagttccc
     2341 gggtgtattc tcttgctgtg tctagagaaa aaggttcagg ttgtttgaat ggattatgcc
     2401 gatgagtatg ccaccgttcg aaaaaagtgt aggcttcccg gaactggata aagtaagcgt
     2461 tgaatcctac tttaggagcc cagtcaatta tctggcggac attttctaaa ctaactgctc
     2521 cttcgatgca gatcccccgg tggcggtagg cagctttttc ttttatctga actgtaaaat
     2581 cggggctttc ccgggaaggg agtatttctc catcgattcc aggtctgacc caccgacagc
     2641 cgatctcggt taaaaaccgg tatactccta gtaatacact acgaggattt attccggtga
     2701 ttacaccact accacttttt atctcaatag cgatagcatc gtctaatttc tgctcttctg
     2761 tttctgaaca cagaccgggg tagaattttt cacttgttcc cacccagata gcttcttttt
     2821 ctaattggtt gaaactctct tttttttcta aaactactga tgtatcttta cccaggatac
     2881 gctgaaggta tttcttaagt tcatctgcgg cgaactctac cgttttttca ctacctaact
     2941 ggtagactct aaaaaaaggt agagaggtct ttttatcagt atttgggata tctacttctt
     3001 tgtggttcat cttttttcct ccctttcaaa ttctggaaat ttaacccttg gttgctccaa
     3061 ctgctacacc ttgagtaata cgatttcgga gaaccaggta aaaagtaaca gtaggtactg
     3121 ttccaatgac cagggcagca aattgctttc catagtcaga agccagtgct cccgaaaatt
     3181 tcatgattcc taccggtaat gataaaaggt tacctcggga gaccagaatg tttatgagca
     3241 tgaattcatt ccagattccc aggccattta atatggctac tgtagcaatt actggtttag
     3301 ccataggtat tataatatag atgaaaatct ttaagtaact cgctccatca attcttcctg
     3361 cttctataac tgaatcaggt agacctttta taaactcggt acagaggtaa atgccgagag
     3421 gaaggcccag cccgatgtat ggtattagca ccccgacgcg ggtattaacg aggccagtgg
     3481 taaaagccat taaatacagg ggtatcataa gggattcgat ggtgaataaa attcctatta
     3541 gaaagcctcc gtacaggaaa ggagtaaacc tggatttaat tttagcaaaa gcaaatgcag
     3601 cagccaggga aaaccatatt acaccgatgg tggatatgcc ggtatacagg atgctattta
     3661 aaaacagagt agaaaaatta gccagacgcc aggccagaga ataatttttg gcaaaccatc
     3721 ggttgggtaa tcccaggggg ttaaggagaa actcctgagt agttttgaaa gaatttatag
     3781 ccatccatat aagggggtat aaagttagaa aggtaaaacc taaaagcaac acatacagaa
     3841 ctatccgggg gagggggcca atttcttctc ttaaactacg ttccatcgaa cttttcttct
     3901 cttttcctct cgatttgtaa ctcactgaag ccttgctcat tcttctctac ccccatatcg
     3961 gttttctact gctttagtga tgataattaa aatgatgctt aatatcacga taaaaataga
     4021 aatggcactg gctttaggat aatttggcgc tcctcgaaat gcagtatcat acatataaag
     4081 agctaatact gatgttctac gagccgggtt accttgagtc atggcgaaaa tgagattgaa
     4141 gcttttcaga gaaccagaaa tagcaagaat gatggtggtt acaattactc ccgaaagagc
     4201 ggggagtatc acatgccaca aagtctggaa ttcggtagct ccttcgattt tagcagcttc
     4261 caggatagtg ggttcgattt tctgtaaatt ggcaagaaag ataattaaat aggttccagt
     4321 gtacatccat aacatcacaa agagaactgg tattatcgct cgcttggggt caaggagaaa
     4381 agtacttcgc catccaggct ggaaatgttg cattatttta gtaagcgggc cataaggaga
     4441 gaaaaatgtc tgccataaga taccaactac aatgggtgaa ataacagctg gcaaataaat
     4501 catggtttgg aagaaatctc caaagcgtac cagttttctg aaaattacat aagcaaaaat
     4561 aaaacctaat ggaatctgtc ccaggactga aatcaatatg atgtagacat tgtttttgag
     4621 cgaaatccag aaataagggt ctttgaacat gctgacgtag ttatggagcc ctacaaattt
     4681 aaaatcgaag gcaaatattg gcccaccact gtagtcggtc atgcttaaca caatagagaa
     4741 taaaagggga aaggctgtga ctgcccagta tataactacc gcgggtaaaa ctaacagcca
     4801 gtatgctcga cgctcctcgc tttttgagta aagatgtggt ggattcacca cttcacctcc
     4861 gcaaaggata atttaacttt attttcaaag tagaaatcaa gaggagaggg tagcctggca
     4921 cgtgttgcca ggctaccctc aaaattagtt atctacattt ggcttttcta ttctgctctc
     4981 cactcttcgt aacctttttg aactttactg gccacatctt ctggtgtaga cattcccagt
     5041 gcgatttcct gaagcccggt gttgattggt aaatatattt tgggatccag aacatcgtca
     5101 agaacgtaag ttccaacata cttttcgtaa aagccaagtc gctgctgaac aagaggttcc
     5161 agcccttcag cggttacgcc ctttctggag ggaatagcag caccggtttc cagacggatc
     5221 ttctgtgcct caggagatac aagccactta atgagtctcc aggctgcttc ctctttagga
     5281 gaaccgtctg gtatagcagc actcatacca aagccggtag ctaaaaccat ggaagtgcta
     5341 ttgggagtta cttcacctgg aatagcagga aaaatggtga gaaggatgtt attctgtttt
     5401 tctggtggga taagtgcttc accggttgtg gggtcagtga gaaacgctcc cactctccag
     5461 tcgccatcga tagtgagcgg agcttctcca gttgcaaaca gggcaggtga tgttccatag
     5521 ggggtcataa caatgtctgg ggtgagtaca ccatcatcaa aaagctgctg ataaaatttg
     5581 agaacagaaa cgaaaggttc atcagtaaat ttggcttctc cagcaactaa cttatctacg
     5641 taatcattgc ctaaaagacg tccgaccagc atactgaaaa gaactgattg tattacccag
     5701 tcatcctgag cacccatcac cactaaactt ttacctgctt ctttggcttt ggaaaccata
     5761 tttttcagct catcgtaggt ttgaggtaca gacacacccc attcatttaa catttcctgg
     5821 tttacataaa gcatgtgagt agcagtaata cctatcggaa tcatagccaa atatttacca
     5881 cgctgattat tgggatctac ggcagcggga acaaaatcgt taatctcatc gcctaagaag
     5941 gggcgcaagt cttttactaa ttttttgtcg tggagagttg cagaacgcgc gccaggccac
     6001 atatagataa catcaggaag attacccgca gcagcataag cttctaattt ttgatggaaa
     6061 ggttcaccaa agagatcctc tcgttcaatc ttgatatcag gattattttt ctcgaaagct
     6121 tcccatatct cttttatttc tctttctgta ccgggcgagg tgaggtctag atagttaaga
     6181 acttttaaag taatttgctc ttgagctgat agcccgctta tgcctgcagt tataagaaaa
     6241 acagccaata caatcgaaat cactcgggat ttcttaatat acatatagat acctcctttt
     6301 aaattccaaa atcaagcact tttaccttct aatggaatta acaccgtttg tccaacgcca
     6361 ttatcctttt aagtacttcc ccgattttta catcacctcc gttgtttttt caggtaatca
     6421 gtaaaggctg acaatctgtg cctttgcttt aatgactcct ggctatatgg gagaagctaa
     6481 ccagcggtga atgacaagac ccactgcgcc cagtaagtcg ctcgactctc ccaaacaaga
     6541 cccggttact ggaactatcc tgccaatacc agatctttcc catctatcca tggttttttc
     6601 tctgatgagg tttaaaaagc catcgcccag tgctccccat gcgcctccaa cgaccactaa
     6661 atccagatct aacagtccag cagcgaacaa aacggcaata gataatgctt ccgcagcctc
     6721 gtctattatt ttgcgtgcta ctggatcacc ttcctggact ttttctttta gatgggaaat
     6781 actttctaga cgattgcctg tttcttcttc atatcttctt ttgatatcag gggcataaag
     6841 ataggtttct aaacatccgt gtagcccaca tcggcatttt ctgccacctg gtataaagca
     6901 tagatggcca atttctcctg ctgtccctcg tggaccggct aacagattgc gttcgaaaat
     6961 tatacctgac cccacaccag tatcagtttt aatgtagaaa aagcttcttg cttcagtggc
     7021 tccgccaaac cagaattctg ccagcgctgc tgcgttagca tcgttttcta ctaataccgg
     7081 taatcccagc tccttttcca gaatttctcc aattttcact tcccgccagc ctaaattgac
     7141 cgaacgtacc agagtctttt gctcactatc cacaatacct gaaattgcta gtcctaaccc
     7201 caggatatat ttccggttga tactagtctc ctgaactaac aaatcgaatt catgcactag
     7261 ctctttaatt aaagattcag gttctaaaga gtagggctga aagtacctct tagaaactat
     7321 ttctcctttc aggtcagcga tggctaaacc tacctgatcc ctggatatac atgcgccggc
     7381 tatataaatc ttttcgtagt taagttgaaa aatcgttggt ttacgtccgg cactggattt
     7441 acccgttcct ttttcgatga ttatcttttg gtccaccagc tcggatgtta ttctgctcac
     7501 tgtagcgggg ctcatatggg taagtttggc taacctggtt tgcgagcagc caccttcccg
     7561 gtaaagagtt cttaaaaggg aagtaacgtt gtataggcga accacacggg tgctgtttaa
     7621 agatttttga ctttttggca tttgtaacac cctatttttt cactatgaaa taattgtact
     7681 ctaagtatag ttcaatataa ttgaaaaagt caataccaaa tttatttcat tatgaaaaaa
     7741 acataaaaga tttgaattga ttttagaata agagtggttt tttagaaatt agcgaaactt
     7801 tttggttatc taaattgaat aatctaaatc ctctttggaa gagtttagag ggttattgta
     7861 aacttatttc tctctcccca ttgacaaatc ccttgctcct gtggtaaatt actcctt
//
LOCUS       DAOSVE010000082         7698 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_4798_length_7698_cov_77.913777, whole genome shotgun sequence.
ACCESSION   DAOSVE010000082 DAOSVE010000000
VERSION     DAOSVE010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 7698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7698
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4798_length_7698_cov_77.913777"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      162..248
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            337..1005
                     /locus_tag="PK016_06970"
     CDS             337..1005
                     /locus_tag="PK016_06970"
                     /inference="COORDINATES: protein motif:HMM:NF023802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HXL02836.1"
                     /translation="MRRRFFFLLTIFGLILIILISGCSSSINTPETSFGISSSPTSGE
                     TQDKFVEFSNDRILLDVPFLPQVPPGDWVNTRNCGPTCAVMMRAYYFNISPSPDHIVQ
                     ADDWLNARFGIPVNGYNSDWTNIFQIRAWLDSEGVPTKVGMGNLEQARAMLSEGKPFL
                     VAIYSNMNSSGGAKHAMLVVGIDSNNIYVNDPGKVTGANNSYPLSQFLSAWSAQGNWY
                     LALN"
     gene            1482..>2271
                     /locus_tag="PK016_06975"
     CDS             1482..>2271
                     /locus_tag="PK016_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02837.1"
                     /translation="MNHKNLFLLVAIITCSLAIMTGCTVSPQFGAPETTGATITGRVA
                     TPESCFSDACDNSSITDGKPLPAATALFYGGDSGQLKIQGETECDGSFGVSAGGEGSY
                     VLYTVREYPEGSSKYVVVKKGINSSSGDVGEANAYTTSQVIIWERANVLYGENFTPFD
                     ASWYAQHNWGWSFDPAKLVLPVSDIPNLEPTAQLYNAVEKALSECRDPQGDNKVIFFA
                     NQIADATFGAPGGGGAGGGGGGTVVTTYTLTTTVSPAGGGTVTGG"
     assembly_gap    2272..2371
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2372..3079
                     /locus_tag="PK016_06980"
     CDS             <2372..3079
                     /locus_tag="PK016_06980"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02838.1"
                     /translation="STTVEATATAGYRFVNWTGDLTGSTNPASITMDSNKSVTANFEL
                     IPPTQYTLTTTVSPAGGGTVTGGGTYDAGASTTVEATATAGYRFVNWTEGTTVVSTTA
                     NFTYTMPDRDVTLTANFEEIPCCVFTLNVEKAEFKWTGGPGGSWKVDFKSKVDNDDCR
                     RRLKVTVEVLKIGGLSQGPKGTYSHEAEGKNFSVNSSIDTGWSLPIVEENWKVKVTIN
                     DTSGDCSDLVLDNIPIS"
     gene            complement(3330..3467)
                     /locus_tag="PK016_06985"
     CDS             complement(3330..3467)
                     /locus_tag="PK016_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXL02839.1"
                     /translation="MNPAQAQKTLEEWCKLAMESQLERIMTFAQTLKKYIVISLIVTL
                     Q"
     gene            3751..5286
                     /locus_tag="PK016_06990"
     CDS             3751..5286
                     /locus_tag="PK016_06990"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HXL02840.1"
                     /translation="MDLVEHIFSGSEIKTLESVVDNAIKLSGAQTGSLILPDSDGSLF
                     VAAGQGLRDEYIGAPIKSDEESVSEHVFKTGEPLIIGSRNYSQFSRRKERKDYSISLP
                     IKKSDGVVVGVFNLNRTGCPFREEDISQLETLTINIAIILEENNLRRNRERVIVALSE
                     IVGFFASLFCTDTSSDVFDKIFYSVKTLTGARKAAVFKLSKKRPYLVFEKDWPKQLRW
                     QALSEVNDQIQECIEQKKIITIEHSSQNLFLFIPLISKNHSPYLFVGVLIEPITIIDY
                     LVLSAVVNMGNTTLENITLYQKSRRLAQERERNRLARELHDGLTQIMVATQIYLHLLG
                     KSLEDTSPNILAILQKVQSLNKLGLEESRFILSELKGKSITSLQFRERVDEVIKLFTT
                     PDKEVEFDYKVNVKEIPYQVYQTVLKILQETLSNIQKYAQAQKVTIHIMNSKQQMMFS
                     IEDDGVGFDPQILEEKESDHFGLKNLKERVRVLRGKFDIDSQPGGGTKIMVKVPLPRE
                     VAV"
     gene            5309..5950
                     /locus_tag="PK016_06995"
     CDS             5309..5950
                     /locus_tag="PK016_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXL02841.1"
                     /translation="MRVAIVDDHPIFAAGLKQLLESDGRYEVRAVAQSGEEAQKTIDF
                     NEIDIALVDVNMPGGGGIELLKAIKRRSKNCRVIMLTVEEDEETINRAMKEGAQGYIL
                     KQESPEHLLKAIQASMDGEILLSSHVYARMVDRIRKTSPPDKEQSIIYSKLTEREIEI
                     TRLIVQGKNNKQIAQELYISESTVKNHISNIMYKLSLKNRTELVAIAVREGIQ"
     gene            6407..6796
                     /locus_tag="PK016_07000"
     CDS             6407..6796
                     /locus_tag="PK016_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXL02842.1"
                     /translation="MATKVKIKQKKVFVVDDNHFFLESLVFLLGRQGDIQVVGTHHRA
                     RGALQKIEATQPDVVILDMQLPDGDGISVLQKIRSCMDIPVIMLTVYEEGREQAMKNG
                     AYAYLIKGGSLDNLYQKIREAGEMRES"
     gene            7313..7660
                     /locus_tag="PK016_07005"
     CDS             7313..7660
                     /locus_tag="PK016_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02843.1"
                     /translation="MTYRVHQVNKKTGVTYVYEATSTWDKEKKQPRNQQVCIGKLDPV
                     TGEFIPSKRLNPIQAAIRDPAVTATAVVVGPTLLLDKITMELELDKILKASFPQVYRE
                     LLSMAYYITRQVI"
BASE COUNT         2213 a         1389 c         1660 g         2336 t
ORIGIN      
        1 taagtcaaac ttttcagcca tctaaagcaa ggactgtttt actactacct gatggatagg
       61 ccgtgtagtt acaaaatttt ttaagttccc ctaaagtttt gaaaaaatct tccgatatat
      121 tataatagta tttaattaga ataattttct attttttaat attttctcaa gggcaaactc
      181 tccgaaagga gaggacgcaa agccacgagc ctaaagcaga ttcttgctaa ggtagtcggg
      241 ttgccggatc tcagcgattg agattcgtta agtaataagt tttagcgcgt cactctcaac
      301 attccttcgg agaaagccct gaaggaggtg tgagagatga ggcgtcgatt tttctttctt
      361 cttaccatct tcgggcttat cctgattatt cttatttcgg ggtgtagtag ttctataaat
      421 actccggaaa ctagttttgg tatctcttct tcgcccacca gtggcgagac acaggataag
      481 tttgttgagt tttctaatga cagaatttta cttgatgtac cttttcttcc tcaggttcca
      541 cctggtgatt gggtaaatac ccgtaattgt gggcctacct gtgcagtgat gatgagggcc
      601 tattacttta acatttcacc atctcctgac catatcgttc aagctgatga ttggcttaat
      661 gctcgcttcg gaattccggt taatggttat aacagtgact ggactaacat ttttcaaatt
      721 cgtgcctggc tagatagtga gggagtgcca actaaagtag ggatgggaaa tctggagcag
      781 gctagagcga tgctttctga agggaaacct tttttggtag caatttattc taatatgaat
      841 tccagtggtg gagcaaagca tgctatgtta gtagtaggta ttgactctaa caatatctat
      901 gtcaacgacc cgggtaaagt tacaggagcc aataattctt atcctctttc ccagtttctt
      961 tctgcctgga gcgctcaagg gaactggtat ttagctttga actaaaatta agcctcctcc
     1021 cggaaaaggc aaagaagatt gttagagaaa aggttatgag gtcttccgca gatttgcgag
     1081 aggatgatac ttcttattat tgaagctatt ttatcctcat gcgtttctct cccttttatt
     1141 tttctctgga gttttccttc aatggcgggg gggaatgtat aatttcttct cttattgttc
     1201 ttttcgttgt tcccgaaatt tagctgggaa ttcatcctct tctaccatgt ctgtaatctt
     1261 taattaggca tctagagata ctttttaaac cataaacccc taataaaacc attcaggggt
     1321 gatagaagaa ggggtaatgg attattcttc ctatagtgat tgtcttgcaa aataggagta
     1381 gtagctacca aaataggaat atttacctat aggattcctc ttttttgggg attattcttg
     1441 ttacagaaaa ggaacttaat ccttaaaagg aggaaataat aatgaaccac aaaaatcttt
     1501 ttttactggt ggccattata acttgttctt tggctataat gactggttgt acggtttcac
     1561 cccagtttgg tgctccagaa acaactggtg caacaataac tggccgggtg gcaactccag
     1621 agagttgttt tagtgatgcc tgtgataact catccataac tgatggcaaa ccccttcccg
     1681 cagctacagc tcttttttat ggaggagata gtgggcaact aaaaatccag ggtgaaactg
     1741 agtgtgacgg atctttcgga gtaagtgcag gcggcgaagg aagttatgtt ctctatacag
     1801 tgagggaata tccagagggt tctagtaagt atgtagtggt taaaaaaggc atcaatagct
     1861 cctctggtga tgtcggtgaa gctaatgctt ataccacttc tcaggtaatc atctgggaac
     1921 gcgccaatgt cctttatgga gaaaacttca ctccttttga tgcttcttgg tatgctcaac
     1981 ataattgggg atggagtttt gaccccgcta aattagttct cccggtgagc gatattccta
     2041 atctcgagcc tactgcgcag ctttataatg cagtagagaa agctctatct gaatgtcgcg
     2101 acccgcaggg agacaataag gtaatatttt ttgctaacca aatcgctgac gctacattcg
     2161 gtgctcccgg cggtggtgga gctggaggcg gaggaggggg aacggttgtt actacctata
     2221 ctctcactac tacagtttct ccagctggtg gaggaacagt aactggtggt gnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcaactaca gtcgaagcta cagcaactgc
     2401 tggttatcgg ttcgtaaact ggactggtga ccttactgga agcaccaacc ctgcttccat
     2461 taccatggat agcaataaat cagtaacggc taactttgaa ttgattcctc ctacccaata
     2521 tactctcact actacagttt ctccagctgg tggaggaaca gtaactggtg gtggaactta
     2581 tgatgcaggt gcatcaacta cagtcgaagc tacagcaact gctggttatc ggttcgtaaa
     2641 ctggactgaa gggacaacgg tagttagcac cactgctaac ttcacttaca ccatgccaga
     2701 tagagacgtc actttgactg ccaattttga agaaattccc tgttgtgttt ttactcttaa
     2761 tgttgaaaag gctgagttca aatggactgg tggaccaggt gggagttgga aagtcgattt
     2821 taaaagtaaa gtagacaatg atgattgtcg tcgacgccta aaagtcactg tcgaagtttt
     2881 gaaaattgga ggattgtctc aggggccgaa aggaacctat tctcacgaag cagaaggaaa
     2941 aaacttttca gtcaactcat caattgatac tggatggtca ttgcctattg ttgaggaaaa
     3001 ctggaaggta aaagtaacaa taaatgatac aagtggagac tgttctgatt tggtgctgga
     3061 taatattccg atttcataat cactaagaac agaggtacct gtcacctgaa ttgttggtca
     3121 atcttgcaac tcctggtagc cggtttgatc taccttacca ggagttgcaa tttttgagaa
     3181 gaaatccacg cattgaatac aaggtgggag atatagcatt gtttctcgag aaaggccaaa
     3241 ctgtgggtca catagaacta cgaaaggacc gagagaatct tgttctttat cctgattttg
     3301 acactttctc tagatttttt gtctcatttc tattgaagcg ttacaattaa ggatatcaca
     3361 atatactttt ttagcgtttg ggcaaaggtc ataattcgtt ctaattgact ctccatagcc
     3421 agcttacacc attcttccaa ggttttctga gcttgagctg gattcatctc ctccagagtt
     3481 ttttcaacgt accttcaagg atatagacga gacaaagatt ctgattcaat tccaacaatc
     3541 tttggagtcg ctgtacttct ttagggagga ggtgctcttt attctttaaa gaaaagcttt
     3601 acttcccttt tcaacttctt ttccttcctc aattgtttat atttgttttt taggctttcc
     3661 tgtcttttgg gggttataag ggggtctttt tccccctata gttctgctat aatattgtaa
     3721 agattttcat ttcttctatc gtggagggga atggatttgg tagagcatat cttcagtggg
     3781 agtgagataa aaaccctgga gagcgttgtt gacaatgcga taaagctatc tggggctcaa
     3841 actgggtctt taatattgcc ggacagtgac ggaagtttat ttgttgctgc tggccagggg
     3901 ctgcgagatg agtatatcgg agctccaatc aagtcagatg aggaaagtgt atcagagcat
     3961 gtgtttaaaa ccggagaacc cttgattatc ggtagccgca attatagtca gttttcccgc
     4021 cgaaaagaaa gaaaagatta ttctatttcg cttcctatta aaaaatctga cggagtagtg
     4081 gtgggtgttt ttaacttgaa tcggactggt tgtcctttta gggaagaaga tatttcccaa
     4141 ctggagacgc tcaccattaa tatcgccatt atcttagaag aaaataatct acgccgaaac
     4201 cgagagcgcg ttattgtggc tctatctgaa atcgtgggat tttttgcttc tttgttctgt
     4261 actgatacct caagtgatgt ttttgataaa atattttatt ccgttaaaac cttaaccggt
     4321 gcgagaaagg cagcagtatt caaactttct aaaaaaagac cttatttggt ttttgagaaa
     4381 gattggccga aacagctccg gtggcaagca ttatcagaag ttaatgacca aatccaggag
     4441 tgtatagagc aaaagaagat tataactatt gaacatagtt ctcagaatct tttcctcttt
     4501 atcccgttga tatcgaaaaa tcactctccc tacctttttg taggtgtttt gatagaacct
     4561 attaccatta tcgattattt ggtgctttcc gctgtggtaa atatggggaa taccactctg
     4621 gaaaacatta ctctttacca gaaaagtaga agattagctc aagaaagaga aagaaatcga
     4681 ttagctcgtg agctgcatga tgggctaacg cagattatgg ttgccactca aatttatctt
     4741 catcttctgg gaaaaagtct ggaagacacc agccctaata ttcttgcgat tttgcaaaaa
     4801 gttcaatctt tgaataaatt gggtttagaa gaatcgcgct ttattctttc tgagctaaaa
     4861 ggtaaatcaa tcacttcttt gcaattccga gagagagttg atgaggttat caaactcttt
     4921 accactcctg acaaagaagt ggagtttgac tataaggtta atgttaaaga aattccttat
     4981 caggtttacc aaacggtgct gaagatttta caagaaactt tatccaatat tcaaaaatat
     5041 gctcaggctc aaaaagttac tatccatata atgaatagca aacaacagat gatgttctct
     5101 atagaagatg atggagtagg ttttgaccct caaattttag aagagaaaga atctgaccat
     5161 ttcggtctta aaaatcttaa agaaagggtt cgggttttgc ggggaaaatt tgatattgac
     5221 agtcaaccgg gagggggaac aaaaattatg gtaaaagttc ccttaccgag agaagtagcg
     5281 gtttaaactt tggtatagag ctatgggaat gagagttgct attgttgacg accatcccat
     5341 ttttgctgct ggcttaaagc aattgctgga aagcgatggt cgctatgaag ttagggcggt
     5401 ggctcagagc ggcgaagaag cacaaaaaac tattgatttt aatgaaattg atattgcttt
     5461 ggtagatgtc aatatgccag gagggggtgg tattgagtta ctgaaagcca tcaaacgaag
     5521 aagtaaaaat tgccgagtga ttatgttgac ggttgaggaa gacgaagaaa ctattaatcg
     5581 ggcaatgaaa gaaggagccc aaggatatat cctaaaacaa gaatcacctg agcacttgtt
     5641 gaaagctatt caggcttcta tggacgggga aatactcttg agtagccacg tctacgctcg
     5701 aatggtcgat agaatcagga aaaccagccc acccgataag gaacaatcaa ttatatatag
     5761 caaattgact gaacgagaaa ttgaaatcac ccggttgata gttcaaggga aaaacaataa
     5821 gcagattgct caggagttgt atatcagtga aagtacggta aaaaatcata tcagtaatat
     5881 tatgtataag ttatcgttaa aaaatcgaac agagctcgtt gccatagcag taagggaggg
     5941 cattcaataa tagatgcaac aaaaattaag tctttccctt tcctcagtat tatttgggtt
     6001 ttgtattttt tatcttttcg ggggttgata agggggtaaa cccccttatg gttgatagag
     6061 gagttcagga gtggcagagg gccattgcgg tgacaaaaaa gaaaggggtt tgtaagggga
     6121 aaagaattcc cccttgcacc gaggaagacg cttttccttc ctcggtgtct tttccttttt
     6181 atattttttc gattcttttc tgcttgcgcc gcaggctgtc tgttttttct tcctcagcta
     6241 ttttttgcct ttaaggggtg ataggggaac acatttatca ttttccaata tagtagtact
     6301 tggggtgctt gaaataggaa taaattccta tagaaagtaa attatttatt ttttataatg
     6361 atagctaatt aaggtttaga actgcaagtc ctttggggat gataagttgg caacaaaggt
     6421 taaaatcaag cagaaaaaag tctttgtggt ggacgacaat catttttttc tggaaagcct
     6481 ggtgtttctt ctcggccggc aaggagatat acaagtggta ggcacgcatc accgcgccag
     6541 gggggcgttg caaaaaattg aagctaccca acccgatgtt gttattctgg atatgcagct
     6601 tcccgatggt gatgggatta gtgtccttca aaaaatcagg tcttgcatgg atattcccgt
     6661 tattatgctc actgtttatg aggaaggtag agaacaggct atgaaaaacg gagcctatgc
     6721 ttatttgatt aaaggtggaa gcttagataa cctttatcag aaaattcgcg aggctggaga
     6781 gatgagagaa tcttaaaaac aaaagttctt tgctagagag ttggctatag gacctcattg
     6841 tagtgcttgc tttatttagc gtttttctta tctgtactgg gttgtgtgaa aattaatctc
     6901 ttttgtattt tatttattat tctattccct ctgccttatt taccattgtt aatttttgac
     6961 ataaccataa ggaggttaaa ctcctttagc ccaacttgca gctttttctc ataaaaactc
     7021 ctatttttaa acatatctaa ttgtagaacc cttgatttat caacctctct tatctaaaaa
     7081 cctcttgtag tcccagaatt attatattat acttgactat accatagaga tagtttatac
     7141 tatcattatg ttcattaaag ccataattaa gacagataag aaaactaaga aacgttacta
     7201 ctcctataga ctatgtgaaa gttatagatt aggtggtaac ccccaccatc gcacgatcct
     7261 taacttacct ggtttcccaa gtattttata actatgaaag gagagtttat gcatgactta
     7321 ccgtgttcat caggtgaata agaaaactgg tgttacctat gtttatgaag ccacctccac
     7381 ttgggataaa gagaagaagc aaccccgtaa ccagcaggtc tgtataggca agctggaccc
     7441 ggtaactggt gagttcatcc cctctaagcg actaaaccca atacaagcag ccattagaga
     7501 tccggcagtc accgccaccg ccgtggtggt agggccaact ctgctactgg ataagattac
     7561 aatggagctg gaactggaca aaatactcaa agcctctttc ccccaggtat accgtgaact
     7621 cctgagtatg gcttactata tcacgcgtca agtcatctaa aatgtaaccg aagaggtatt
     7681 ggttgggtca tttaaaaa
//
LOCUS       DAOSVE010000083         7492 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_4916_length_7492_cov_71.024069, whole genome shotgun sequence.
ACCESSION   DAOSVE010000083 DAOSVE010000000
VERSION     DAOSVE010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 7492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7492
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4916_length_7492_cov_71.024069"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            427..1353
                     /locus_tag="PK016_07010"
     CDS             427..1353
                     /locus_tag="PK016_07010"
                     /inference="COORDINATES: protein motif:HMM:NF021597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HXL02844.1"
                     /translation="MREIRVIMLISFLVILSPPLYADMGAIVPAGEVKVEEMAQKAII
                     AHDGFEEILILSTDLIVSQNTPVLRLIPFPSLPEIIEVDREIWERLSYICKKHNLQYL
                     TFYKGGEKEEEIKVEYQVTQGAHEITVVKVNDGEEFLEWVRNFLLQNSLPSRFQEEKL
                     QSVVDDYLKRGINYFVLDIVDFQENRERVEPVGYAFLSRNFYYPVKISSLFEGSGEVE
                     LFVLSDRGEMLENFINFWPGWSGGGMSSTAVLEEEDLEFLHPEIHHLLGKRGVLCAFR
                     HPRFQELQEDIIFEIPVEEIKPQGFSNFIKKN"
     gene            1364..2230
                     /locus_tag="PK016_07015"
     CDS             1364..2230
                     /locus_tag="PK016_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HXL02845.1"
                     /translation="MLLQTFCHVPGIGEKIERRIWKEGVITWEEALDYPALEKIIPSP
                     FSRSARSFLYRSLQKLERGEAHFFKEHLDSRDHWRLFPHFPNDTVFLDIETTGLNPPQ
                     DYVTAITIFDGKRIKTFIQGINLPEFKEEIMRYKVIVTFNGKCFDIPFLEQHLGIKFP
                     QIHLDLRFILRSLGFRGGLKACERALGIDRGELRGVNGYTAVLLWQKYREGCLEALET
                     LLAYNVADAVNLEKLMVFAYNEKLRNYPFPQLALSLPPKKIITPYQVHPEILREINHE
                     YWRAIAQKEVEV"
     gene            2263..4446
                     /gene="glgB"
                     /locus_tag="PK016_07020"
     CDS             2263..4446
                     /gene="glgB"
                     /locus_tag="PK016_07020"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HXL02846.1"
                     /translation="MESIINADCHDPFSILGMHPLEKEKGVCVRVFIPAAKKIKVIDR
                     YDSQNSWLMDKVEERGFFELTLPGKDFFAYFLEIEEEEGVRVTADPYSFLPFFSEFDL
                     HLFNEGTYHFAYERLGAHVLEVGGIKGVYFSVWAPNARRVSVVGDFNKWDGRIHQMRV
                     LGNSGVWEIFVPQAGEGAKYKFEIKTREGNLLLKADPYAFFSEMRPATASIVYNLSPF
                     SWKDEEWMERRKSHNLLSSPWSIYEVHLGSWRRGEDNSFLNYRDLAHQLVAYAKEMGF
                     THIQLLPIAEHPFDASWGYQVTGYFAPTSRFGTPEDFRYFVDYCHREGIGVILDWVIA
                     HFPKDAHGLGRFDGTALYEHLDPRRGEHPHWGSYIFNYGRNEVRTFLISNAFYWFKEY
                     HIDALRVDAVASILYLDYGRNEGEWIPNPYGGKENLDAIGFLRYLHENVYRSFPGIAT
                     IAEESTAWPGVTLPPYLGGLGFLFKWNMGWMNDFLTYISKDPIYRKHHHQNLTFPLLY
                     AFSENFILPISHDEVVHGKKNLIDKMPGDWWQKFANVRLSFATMLGYPGKKLMFMGVE
                     FGQWREWNHDRELDWFLLGYETHRQLQRLVKDLNHLYITERSLFELDYSWQGFEWIDC
                     NDADNSVISFVRKSKDSGDFLVFVSNFTPVPRFNYRIGVAREGFYQEILNTDSEVYGG
                     SNLGNWGGVWTEDIPAHGRPFSLNLTLPPLGSLIFKWREHPVLNR"
     gene            4479..6128
                     /locus_tag="PK016_07025"
     CDS             4479..6128
                     /locus_tag="PK016_07025"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HXL02847.1"
                     /translation="MKILEGLALRFLIEEIEKEIKGARIQRVFTTTRKETWLEVYSFQ
                     EEKSLVISLHPQVSFIFTTSCREAQKEKPDNWQQLLNKYLVGGKIISLAQVGWDRVVR
                     IEIKNPHLFEEGNLYLFVELTGRNTNAILTDQSEPPHILGVYRRVDEEQNRFRVLLPG
                     EKYSLPPQKDKISPLFFLEEKDLSLSYSPEELQYKLIQQIDGIGPFLSQVIVEKVEEG
                     ENIQGAIKEIISPLLQNGYKIKVFFSSSGEPQGIFWGSATSYSSLSYKEFPSFNQAVE
                     CFHFEWWKRQEEFIRQKIKERRLKEELDFLKREREKIESLMKDEEELEDLKIKGELLK
                     VLPGLEVISRTEEGIMVRNIFSSPPQNIFISLNPRLSLNQNMQNYFRLYRKGRERNLK
                     LRERLKGLEEKERRVEKIFEEEPSFEKEEDKTSVSERALRFKTSWGSEIWVGKNAKSN
                     HFLVTRIASPQDYWLHVRDLPGSHVILKTKSRENEEEEIQKAAQVAAYFSSVRGESRV
                     EVILTQVKYVRPLPSQIGKVTYRKEKTLLVKPQLPGDVSPW"
     gene            6767..7234
                     /locus_tag="PK016_07030"
     CDS             6767..7234
                     /locus_tag="PK016_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HXL02848.1"
                     /translation="MPRCRRMGPPWWEPRFQEAGFRLTMPRQAILEVLSNTEEHLSAE
                     EIYLAVHKIYPNVGLTTVYRTLELLVDMGLVFKFDFGDKRARYELSQGPMSKKHHHHL
                     VCTNCGRIIDYTDFIDEEKELLRRTEAGLSEKYNFKITNHLIQFYGLCEECQG"
     gene            7297..>7492
                     /locus_tag="PK016_07035"
     CDS             7297..>7492
                     /locus_tag="PK016_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HXL02849.1"
                     /translation="MPGGDRTGPWGLGPMTGRAAGYCAGYSVPGYMNPIPGRGWGAGF
                     GRGWGRGYFGRGRGWRNWYRA"
BASE COUNT         2239 a         1285 c         1685 g         2283 t
ORIGIN      
        1 tcataagggg gaattctttc ccccttatac tgaggaagac gttttttctt cctcacgctg
       61 gagtgttttt ctccagcgta attggtttta gtttctttca tctattttgc tttcgtcttt
      121 ggtgccgcag gtactgagga agacgttttt tcttcctcag ttgtttttgc ctctttaagt
      181 cttttgtctt ttttttgctt tttgggggat tataaagggg ttctcccctt tatagttcgg
      241 ggttatgagg gggcagcgcc ccctcatagt ctttgcggtt aactataaaa taaaaagctc
      301 gaaatgatta tgctgaaggg aagagtgtct tcaagtaagg taaggatgat tctgtttccc
      361 attgctattg gaaaaatttt taaattattc tacaatataa caagtagaat tagaaaggag
      421 caaagaatga gggaaattag agttataatg cttatctcct ttctcgtgat tttatctcct
      481 cctctttacg ctgatatggg agccatagtt ccagcaggag aagtgaaagt agaagagatg
      541 gctcaaaaag ctattattgc tcatgacggt tttgaagaaa tccttattct ttctactgac
      601 ctcattgtct ctcagaacac tcctgttttg cgcttgattc cctttccttc tcttccagaa
      661 ataatagaag tagatcgtga gatatgggag aggctttctt atatctgtaa aaaacataat
      721 ttgcaatatt taacatttta taagggggga gaaaaagagg aagaaataaa agtagaatat
      781 caggttactc aaggggctca tgaaattaca gtggtaaaag taaatgatgg tgaagaattt
      841 ttagaatggg tcagaaactt tttgctccag aatagccttc cttctcgttt tcaggaggaa
      901 aagctccaga gtgtagttga tgattactta aaaagaggaa taaattattt cgtcttagat
      961 attgtggatt ttcaagaaaa cagagagaga gttgaacctg taggttatgc ttttctctct
     1021 cgaaattttt attatccggt aaagatttcc agtctttttg agggtagcgg agaagtagag
     1081 ctttttgtcc tctctgaccg gggagagatg ttagaaaact ttattaattt ttggccagga
     1141 tggagtggag gagggatgtc ctctactgcc gtattggaag aagaagattt agaattctta
     1201 catcctgaaa tacatcatct cttaggtaaa agaggggtat tatgtgcttt tcgtcacccc
     1261 cgttttcagg aattacaaga ggacataatc tttgaaatac ctgttgaaga aataaaacca
     1321 cagggatttt ctaactttat taaaaagaat tgagggaaaa gagatgcttt tacaaacttt
     1381 ttgccacgtc cccggtatcg gggaaaaaat agaaaggaga atctggaaag agggtgtcat
     1441 tacctgggaa gaagctctgg actatcctgc cctggaaaag attatccctt ctccgttttc
     1501 tcgctcggcg cgttctttcc tttatcgttc tcttcagaaa ttggaaagag gagaggctca
     1561 cttttttaag gagcacttag atagtcgtga ccactggagg ttgttccctc attttccaaa
     1621 tgatactgta tttttagata ttgaaacaac cggtttaaat ccccctcaag actatgttac
     1681 tgccataact atttttgatg ggaaaagaat aaaaactttt attcagggga tcaatctacc
     1741 ggagttcaag gaagagatta tgagatataa agtgattgta actttcaatg gtaaatgttt
     1801 tgatatcccc tttttagagc agcatttagg aataaaattt ccgcagatac atttagatct
     1861 ccgctttatt ttacgaagtt taggttttcg gggagggctg aaagcttgtg aaagagctct
     1921 gggtattgac cgaggggagc tgcggggagt gaatggctat actgcagttc tactgtggca
     1981 aaaataccgg gaggggtgtc tggaggcatt agaaacactt ttggcttaca atgtggcaga
     2041 tgcggttaat ttagaaaaat tgatggtttt tgcctacaat gaaaaattac gaaattaccc
     2101 ttttcctcag cttgctcttt ctctccctcc taaaaaaata attactccct atcaggttca
     2161 cccagaaata ttgagggaaa taaatcatga atactggcgt gccattgccc aaaaggaggt
     2221 ggaagtgtga gtaattcacc agttactatc agcaaagaaa aaatggagtc cataatcaat
     2281 gctgattgcc acgacccttt ttccatactg ggaatgcatc ctttagaaaa ggaaaaagga
     2341 gtttgcgtaa gggtttttat tcctgcggct aaaaaaataa aagtgataga ccggtatgat
     2401 agccagaata gctggcttat ggataaagta gaagaacgag gttttttcga gctgacccta
     2461 cccgggaaag atttctttgc ctacttttta gaaatagaag aggaagaagg agtaagagtt
     2521 actgctgacc cttactcttt tcttcctttt ttttctgagt tcgatctcca ccttttcaat
     2581 gaaggcactt atcactttgc ctatgagcga ttgggtgctc atgtacttga agtaggaggg
     2641 ataaagggag tctattttag tgtttgggct cctaatgctc gccgggtaag cgtagtagga
     2701 gatttcaaca agtgggatgg acgtattcat cagatgagag ttctgggaaa ctcaggggtg
     2761 tgggagatat tcgtccctca agcaggggaa ggggcaaaat ataagtttga aatcaaaact
     2821 cgagagggga atcttcttct taaagctgac ccttatgctt ttttctctga aatgcgtccc
     2881 gctactgctt ctatagtgta caatctttca cctttttcct ggaaagacga agaatggatg
     2941 gaaagaagaa aatctcacaa tctactttct tctccctgga gtatttatga agttcactta
     3001 ggctcctgga gaagagggga agataattct tttttaaatt atagagatct ggctcatcaa
     3061 ctggtggcct atgccaaaga gatgggattc actcatatcc aacttcttcc cattgctgaa
     3121 cacccttttg atgcctcctg gggttatcag gtaactggat attttgctcc caccagtcgc
     3181 tttggcactc cagaagattt tcgatatttt gtagattact gccatcggga agggataggg
     3241 gtaattttag actgggtaat tgcccatttt cccaaagatg cccatgggtt aggaagattt
     3301 gatggtactg cactgtacga acacttagac ccccggcggg gagagcaccc tcattggggg
     3361 agttatattt ttaactatgg aaggaatgaa gtaagaactt ttttaatctc taacgctttt
     3421 tactggttta aggaatatca tattgatgcc ttacgggttg atgcagtggc ctctatactt
     3481 tatctcgatt atggtagaaa tgaaggagaa tggataccca atccttatgg ggggaaggaa
     3541 aatttagatg ctattggttt tcttcgctat ttacatgaaa atgtttaccg cagttttcct
     3601 ggaattgcta ccattgctga ggaatccact gcctggccgg gggtaacact gcccccgtat
     3661 ctggggggat taggttttct tttcaagtgg aatatgggat ggatgaacga ttttctcact
     3721 tatatcagta aagaccccat ttatcgaaag caccatcacc aaaatcttac ctttcctttg
     3781 ctgtatgctt tttctgagaa ttttatctta cccatttctc atgatgaagt ggtgcatggg
     3841 aagaaaaatc tcatcgataa aatgccagga gactggtggc agaaatttgc caatgtgcgt
     3901 ctgagctttg ccactatgct tggttatccg gggaagaagc tcatgttcat gggtgtagag
     3961 tttggacagt ggcgagaatg gaaccatgat cgggaattgg attggttttt gctaggctat
     4021 gaaactcatc gccagcttca aagattagtg aaagacttga atcatctcta cattactgaa
     4081 aggtctctct ttgaattgga ctattcctgg caaggttttg aatggattga ctgtaacgat
     4141 gctgataatt cagtaatctc ttttgtgcgc aaaagtaagg attcgggaga ttttttagtt
     4201 tttgtttcca attttactcc tgttcctcgt ttcaattatc gtataggagt ggccagggaa
     4261 ggattctatc aagaaatctt gaataccgac tcggaagttt atggaggtag taatctggga
     4321 aactggggag gagtgtggac agaggatatc cctgcgcacg ggcgaccttt ttctttaaac
     4381 cttactcttc cacctctcgg ctctcttatt ttcaagtgga gagaacaccc ggtcctgaat
     4441 agataggaag ctataatgcg tttttctcgg tggaactgat gaaaatttta gaaggtttag
     4501 cactccgttt cttgatagaa gaaatagaaa aggaaattaa aggagcacga atccagagag
     4561 tttttactac tacccggaaa gagacctggc tggaggttta ttcttttcaa gaagagaagt
     4621 ctctggtcat ttctctccac cctcaggtca gctttatttt tactacttct tgtcgagaag
     4681 cacagaaaga aaaacctgat aactggcaac aacttttgaa taaatatttg gtaggaggaa
     4741 aaatcatttc tctcgcccag gtaggctggg atagagtggt gaggatagaa attaaaaacc
     4801 ctcatctctt tgaagaaggg aatctttatt tgtttgtaga attaacaggg agaaatacca
     4861 atgctatttt gactgaccag agtgaacctc ctcatatttt aggggtttac cgtcgggttg
     4921 atgaagagca gaatcgattc agagttttat taccaggaga aaaatattct ctacctcctc
     4981 agaaagataa aattagccct ctctttttcc ttgaagaaaa agatttatct ctttcctact
     5041 ctcctgaaga gttgcaatat aaactcattc aacaaataga cggtattggt ccttttttaa
     5101 gtcaggtaat agtggaaaaa gtagaagaag gcgaaaatat tcagggagct ataaaagaaa
     5161 taatttctcc tctccttcag aatggctata aaataaaagt atttttctct tcttctggag
     5221 aaccacaagg aatattctgg gggtcagcca cttcttattc ttctctttct tataaagagt
     5281 ttccttcctt taaccaggcg gtggaatgtt ttcactttga gtggtggaaa aggcaagagg
     5341 agttcattcg acaaaaaata aaagagagaa ggcttaaaga agagcttgat tttctgaaaa
     5401 gggagagaga aaagattgag agcctgatga aagacgaaga ggagttagaa gacctaaaga
     5461 taaaaggaga gcttctgaaa gttcttcctg gtttagaagt aataagtagg accgaagaag
     5521 ggataatggt aaggaacatc ttttcttctc ctcctcaaaa tatttttatt tctcttaacc
     5581 ctcgtctttc tttaaatcag aatatgcaaa attattttcg actttatcgt aaaggccgag
     5641 aaagaaacct taaattgaga gaaaggttaa agggattaga ggaaaaagaa agaagagtag
     5701 aaaaaatttt tgaagaagag ccttcgtttg agaaagaaga agacaagacc tctgtttcag
     5761 aaagagcttt aagatttaaa acctcctggg gtagtgaaat atgggtgggg aaaaatgcaa
     5821 aaagcaacca ttttttagtc accagaattg cctctcctca ggactactgg cttcatgtaa
     5881 gagacttacc cggctctcac gtaattttga aaacaaaaag cagggaaaat gaagaggaag
     5941 agatacaaaa agcagctcaa gtagctgctt atttttcttc cgttcgagga gaatccagag
     6001 tagaggtcat tttaactcag gtaaaatatg ttcgacctct tccttctcag ataggaaaag
     6061 taacttaccg gaaagaaaaa actcttctcg ttaaacctca gctacctgga gatgttagcc
     6121 cctggtaaaa actgtcttct gataatacta actatataca cttctttgtt aagaaccaaa
     6181 aggtgagaat aagaggtaca ataaaatgag tcagcctcgc ctaccttttt attctccctg
     6241 aggttaacta taaaatggca aagaccagat ccagaagtta gaaaagaagc cttaccacca
     6301 aggatagaag gagaattggt attacaattt tgccaggagt taaagattta aaaataaaag
     6361 agccgaccga atatgaggat agctattttt cctgattggt cttttttctt tggtaatcaa
     6421 tggagaattt atcagggttt tcctggtatt ctcgaacaag tcgggaaaaa tcttcttttc
     6481 tcaatccgca aaaggtaagg aagcttcttc ttatccttca gacactctca cagaaacatc
     6541 tttacctctt cagttgagaa tctcttatgt agttgttcag tcatactatc ctcctctttg
     6601 gggtaaattt ctaagaggat aatatgcaat attatattct ccaggcaaat tagagagatg
     6661 aaattttaaa ctttaaatga caacagcctc ctcaagagtt gacaaatttt ggttgctaat
     6721 ctataatgtt aatgaaaacc attttcaata atgagaggtt ttaagtatgc cgagatgtag
     6781 aagaatgggg ccaccctggt gggagcccag gtttcaagaa gctgggtttc ggctaactat
     6841 gcccagacaa gcaatattag aagtgctcag caatactgaa gagcatttaa gtgcagaaga
     6901 gatatattta gcagttcata aaatttatcc taacgttggg cttactacgg tttaccggac
     6961 tttagagtta ttggtggata tgggattggt gtttaaattt gattttggag acaaaagagc
     7021 gagatatgag ctatctcaag ggccgatgag caagaaacat caccatcatc tggtatgtac
     7081 caattgtggc cgaataatag attatactga ctttattgat gaagagaagg agctactccg
     7141 gcgaacagaa gcgggacttt ccgaaaaata caacttcaaa atcactaatc atcttattca
     7201 gttttatggc ttgtgcgagg agtgccaggg atagcttaaa aaatttttag taataaatga
     7261 aaatggtttt caatattaaa aagggaggtg tttcgaatgc caggtggcga tagaactggt
     7321 ccttggggat taggtccaat gactggaagg gctgctggtt attgtgcagg ttattcggtt
     7381 cccggctata tgaaccccat tcctggtcga ggatggggag caggctttgg ccgaggttgg
     7441 ggtcggggtt actttggtcg aggtcggggt tggagaaact ggtaccgggc ca
//
LOCUS       DAOSVE010000084         7348 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_5012_length_7348_cov_81.771699, whole genome shotgun sequence.
ACCESSION   DAOSVE010000084 DAOSVE010000000
VERSION     DAOSVE010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 7348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7348
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5012_length_7348_cov_81.771699"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            672..1526
                     /locus_tag="PK016_07040"
     CDS             672..1526
                     /locus_tag="PK016_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1616 domain-containing protein"
                     /protein_id="HXL02850.1"
                     /translation="MIVKNELVIIDMISAILVTLILFTEERILRIILGLPFILFFPGY
                     VLIAALFVRKKDLSSIERVALSFGLSIAIVPLIGLMLNYTPWGIRTTPVLISLFLFIL
                     LLSCVAIFHRSRVPEEECFCLSLQMISTAKEKNSLLDRALSIILIISILLAIGTLIYV
                     IATPKVGERFTEFYLLGPGGMAEGYPRELAPGEEGKVIIGIVNREYEEVTYTITVRAI
                     QETGESKKEKSLSILGPFTLKHEEKWEKPFVFSLSEKGENIKIEFLLFREDKEEPYLS
                     LHLWVTVS"
     gene            complement(1536..2711)
                     /locus_tag="PK016_07045"
     CDS             complement(1536..2711)
                     /locus_tag="PK016_07045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXL02851.1"
                     /translation="MRREYQVIVVGSGPAGSVAAYELAKKGIEVAILEKQRLPRYKCC
                     AGGVSVGAAKLLDIDLQGLVENTITRVYISFQGKTPFHGEHSSPLMYTVMRDRFDYAL
                     VQRAKEAGAAVFEESEATQIKINDKGIEVSTSGGDFSAQYLVGADGANSLVARELKLQ
                     TRREWIVAIQREIEVEEEILAKVKSQVILDFGRVNRGYAWIFPKLQHLSVGMACLSSG
                     AKNLKKHYHDFLVSYGLDKEITLREDTALLPISRGNPIVCEGRTALVGDAAGLVDPLI
                     GEGIYNAIWSAQLAAQTITLAIAEGNSDLLSYQQAVNQHIVPELEIAQKFSRLFATFP
                     ALFFEMLKREEIVWRGCRFFLRRDMNYSSIMQGLGGLRGLMKLLLKTRDGGKIRDKD"
     gene            complement(2712..3260)
                     /locus_tag="PK016_07050"
     CDS             complement(2712..3260)
                     /locus_tag="PK016_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02852.1"
                     /translation="MKNVQLGTTILRLIVFSIVFYSGLVAGSGQLIAQSLGDFFSFTY
                     QAQLSETEISKGETFFALIKSEVIYKGNLPISPRGARITSRIVARRELDNNEAGNDII
                     LNPGYTLNVNPFPRRGEQIELSSTVPLQFPPESEPGAYQVVGVLESARLIIGPISISA
                     TRYLPQLQVLGKVSYTGLQEEH"
     gene            complement(3517..4026)
                     /locus_tag="PK016_07055"
     CDS             complement(3517..4026)
                     /locus_tag="PK016_07055"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HXL02853.1"
                     /translation="MLLFGHLGITLGSFEAGASVYRWLTKKPLNLMFYYPTILIGSML
                     PDIIDKPLGGIILKDTIGNGRIYAHTLLFLLSLFVIGGYFWFRFHSNTGIITALGCLG
                     HDILDELWLQPDTFLWPAFGWSFPKENSQQWLHTWLAELMSDPGVYIPELIGFTIFLV
                     LIYHYQKVK"
     gene            4597..6396
                     /locus_tag="PK016_07060"
     CDS             4597..6396
                     /locus_tag="PK016_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HXL02854.1"
                     /translation="MKNFVAIMLLILFVFTLPVLANPLADVPLNHWAYDAVQSLAASG
                     IVIGYPDGTFGGNRTLTRNEFAVAIARAIGYLESSMDSANLATQEDLELLEKLIQEFA
                     DELQKLGVTVEDIKNVVGENSQAIQALNSRVSELESYAEPLKVTGELTTKYTGYVPAD
                     PDSTEKEGCEKWPKTGYRLEDQVDFHLLATINEFTTSGLTLTVTDTFTDNPSVSADHF
                     YVEYKQDPWYIRFGRVQLKRFDLGLVLGLYRPADPESSYALDFDGFYIAYQPEGSEVA
                     WKAVGSPNDFYMVRAEWEDTALALTWMPEGDELYSPENSDLIVSAAMKTDFNQSDVRM
                     TLEGAYGVLSDTYGLAGELAFKVKENATITLSGRYITEGFTPVSSQYIEWVNDTCGTE
                     QRISRWDASNRMIFTLPGNFQLSGQGKDQEQWKLSFGYTYGEKTTTGEKISDEVNATL
                     TYIPAQPVSGESATLRVNYYMMKDNYGVFGGYRNYPLEIQNESQEAYISANVQYVPDS
                     SEITAVGELRYRWLEEKTTLTFQGRYDSSPKKEDFSTWGLLTNLEWEMAKNTRLSLGY
                     ELNTWDGSLFAYDDDRGIIDKAGTMKAELKVSF"
     gene            complement(6489..6692)
                     /locus_tag="PK016_07065"
     CDS             complement(6489..6692)
                     /locus_tag="PK016_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012899030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methytransferase partner Trm112"
                     /protein_id="HXL02855.1"
                     /translation="MKKELVDILACPVCKTNLELTIKEKNASGEVLEGSLYCRKCGHN
                     YSIKEGIPNLLPPSLDRKDNAPL"
BASE COUNT         2112 a         1552 c         1483 g         2201 t
ORIGIN      
        1 gatggaagac cacagctact tcgtcgggca tattaacagc agtaaaattg gatttgtgat
       61 tttatcagtc tttcttagct ctaagacgct ttcccagctc tcaggttgcg gaagttttac
      121 tacttgcccc acaaaaatcg cctctttctg ggagtcgatt ttatagtcga cggactcgtg
      181 cttttagaga aagtgggaaa tcacaagaga ggctctatag agctctggtg agccgtgcag
      241 gaatcgaacc tgcgacaccc ggattaaaag ttgtgggcat agggtttttt atacacttta
      301 cgatgtcctg aaggtgctta aaataaggat tttttatttt ggagtttttt aaattcttta
      361 ctggttgctg tcaaaattgc tgtcaaggta aaaaaatgat ggagcaaaaa attctacgct
      421 gaagagggtt gatttatttt ggcatactac caaattttct atgggttgct tcaaaaatta
      481 ccagctggat ctattcttta ccaccatact tctttttaga tatttttttc cacacaattt
      541 ggtatagagc caaatagcca ttgaaatcag aaaaggattc tgggataata agaaagattt
      601 tgcaggctcc agggaaatgt gaaaagaaaa catcagaata agaaaaatgc tcaagcaaaa
      661 ggatgctcaa catgatcgta aaaaatgaat tggtcataat tgatatgatt tcagctattc
      721 tcgtaactct aattcttttt actgaggaac gaatactacg aatcattctt ggtcttcctt
      781 ttatcctttt ctttcctggt tatgtcctta ttgctgcttt gtttgttagg aaaaaagacc
      841 ttagctctat tgaacgagta gctttaagtt ttggcctaag catcgctata gttcctctaa
      901 taggtctaat gttgaactat accccctggg gaataagaac cacacctgtt cttatttctt
      961 tgtttctttt tattttactg ctttcttgtg tagcaatttt ccaccgttct cgagttccag
     1021 aagaagagtg cttttgcctc tccttgcaaa tgatctctac agctaaagaa aaaaattccc
     1081 ttttagaccg agcgctttcg ataatactga ttatctccat tctcctggct ataggcactt
     1141 tgatttatgt tattgccaca cccaaggtgg gagagcgatt tactgaattt tatctcctag
     1201 ggccgggggg aatggctgag ggctatcccc gtgaactggc accgggagaa gaagggaaag
     1261 tcatcatagg gatagtaaat cgggaatatg aagaagttac atacacaatc actgttcgag
     1321 caattcaaga gacaggtgag agcaagaaag aaaaaagctt gtccatttta ggaccattca
     1381 ctttaaagca tgaagaaaaa tgggaaaagc cctttgtttt ttcgctgtca gaaaaaggag
     1441 aaaacattaa aatagaattc ttactctttc gggaagataa agaagaaccc taccttagtc
     1501 ttcacctgtg ggtaacggtt tcataattct tttccctaat ctttatctct tattttaccc
     1561 ccatctctcg tctttaaaag caacttcatt agccccctga ggccacccaa gccttgcata
     1621 attgagctgt aattcatatc ccgtcgcagg aaaaaacgac atcctctcca tactatttct
     1681 tctcgcttca acatctcaaa aaataatgcc gggaaagtag caaaaagccg cgaaaatttt
     1741 tgagctatct cgagctctgg cacaatatgc tggttaacag cctgctgata cgacaaaagg
     1801 tcggagtttc cttcggcaat agcaagagtt atagtctgag cagccagttg agcactccag
     1861 atagcgttat aaattccctc ccctatcagg gggtcaacta atccagcagc gtcaccaact
     1921 aatgctgtcc taccctcgca aacaataggg ttacctcttg agatgggtaa gagtgcggta
     1981 tcttctcgaa gagttatttc tttatctaaa ccgtaggaaa ccaaaaagtc gtggtagtgc
     2041 tttttcaaat ttttagctcc cgagctcagg caggccatcc ccacagaaag atgctgaagc
     2101 ttagggaata tccaggcata acctctattc actctcccaa aatccagaat aacttgtgac
     2161 tttaccttag ccaaaatctc ttcttctacc tcaatttccc tctggatagc aacaatccat
     2221 tcccgtctgg tttgtagttt caactcccga gcaaccaaac tattagcgcc atcagcacca
     2281 acaagatatt gagcgctaaa atcacccccg gaagtggaaa cctctatccc cttatcattt
     2341 atcttgattt gagtagcttc actttcttca aacaccgctg ctcctgcttc tttagcacgc
     2401 tgaaccagag cataatcaaa cctatctcgc atgacagtat acataagagg actagaatgt
     2461 tctccgtgaa agggagtttt tccctgaaag ctaatataaa ctctcgtaat ggtattctca
     2521 accagccctt gaaggtcaat atccagcaac ttagcagcac caacactaac tcccccggca
     2581 cagcatttat agcggggtaa acgctgcttt tctaaaatag ctacctctat tccctttttt
     2641 gctaactcat aggctgcaac tgaccccgca ggtccagaac caacgacgat tacctgatac
     2701 tcccgtctca tttaatgctc ctcttgtaac ccggtatagc ttaccttacc caggacctga
     2761 agctgaggta gatatctggt tgctgaaatt gatattggtc caataataag tcttgccgat
     2821 tccaaaactc ccactacctg ataggcccca ggttcgctct ccgggggaaa ctggagaggg
     2881 acagtgctcg atagttcaat ttgttcccct cttctaggga agggattaac gtttaaagtg
     2941 tagccagggt tcaatattat atcgttacct gcttcattat tatctaactc tcgccgagct
     3001 acaatacggc tggtgattct ggctccccga ggtgatatag gtaaattccc tttgtatatt
     3061 acctcactct ttattagagc aaaaaaggtc tcgcctttgc taatttctgt ttcactcagt
     3121 tgagcctgat aagtgaaaga gaaaaaatcc cccagactct gagcaatcaa ctggccacta
     3181 cctgcaacca atcctgagta aaacactatg ctaaaaacga tcaatcttaa aattgttgtc
     3241 cccaactgca catttttcat aagctatttc cccttttttc gtaaaaatag cttaacaata
     3301 agataaggct acttagtttt ttcacattca cactcacttt cactctcttc tcaatattat
     3361 tttatagcat aaagttcttt ttacaagatt cagtttttgt tatctcatct tatactggat
     3421 agttctattt tttctgtttt aggcttataa ggggcataat cagagtgaaa atacagtaaa
     3481 taaccaggct ccaagcaacc agaaagggtt gaatatttac tttacttttt gataatgata
     3541 gattaacacc aggaagattg taaatccaat gagttccgga atgtataccc ccgggtcact
     3601 cattaattcg gccagccacg tatgtaacca ctgctgacta ttttctttag gaaaagacca
     3661 gccaaaagct ggccacaaga atgtgtcggg ttgtaaccat agctcatcca ggatatcatg
     3721 acccaggcat cccagagcgg taataatccc ggtgttggaa tgaaaacgaa accaaaaata
     3781 acccccgatt acaaaaagac tgagaagaaa aagcaaggta tgggcataga ttcttccatt
     3841 tccaatggta tctttgagga ttatcccacc taaaggcttg tcgatgatat caggaagcat
     3901 tgagccaatg aggatggtgg gatagtaaaa cattagattt aatggttttt tagttagcca
     3961 gcgataaact gaagctcccg cttcaaaaga gcccagagtt attcctaaat gtccaaatag
     4021 cagcattcgc tttcccaagt tctttcctgg attctatacg ttgtcacctt ttttatattt
     4081 caattcctcc caaatttcca gctttttgcc ctttggaata tacatcatga tagcatatat
     4141 aaatattata gttacccaca aaaatctact gtagtgtaca tctatttatt gtttcactct
     4201 tcttagcttt gagtttgaat tgtaacaatc cgttgatgtc accattaacc tcgcctggca
     4261 gcctatggca aaaacttaaa aagcataaaa ggtggagaag gctaaaacca taacgctggg
     4321 gaaatacacc caaaaaaaga ggtaattcaa aacttataaa atagacatac atctggagta
     4381 taaaaaggaa gtatgctcta taaaaatagt acttttctaa cagcttcttg tttacttgac
     4441 attgataaac actttcttgt aatatacttt tgtgaggtat ttcctaaagg ggagatagaa
     4501 aaatctgtag atatgagagg taagaaagag tagaataaat tttgttctta aaaagaagtt
     4561 ttgttttctg tctaagttta ctggagggag gatattatga aaaattttgt agcaataatg
     4621 ttattgattc tgttcgtctt tactctgccc gttctggcca atcctttagc tgatgtacct
     4681 ttaaatcatt gggcttatga tgcagtgcag agcttagcag ccagcggcat tgttattggt
     4741 tatcctgatg gtacttttgg tggcaaccga actttaactc gtaatgaatt tgctgtggcc
     4801 attgcccggg cgattggcta tctggaaagt tctatggata gtgctaatct cgctactcag
     4861 gaagacctgg aactcctgga aaagcttatt caggagtttg ctgacgagct gcagaagctt
     4921 ggcgttacgg tagaggatat aaaaaatgtg gtaggtgaaa atagtcaggc tatccaggca
     4981 ttaaattccc gggtttctga gctggaaagt tatgctgaac ctttgaaagt gactggagag
     5041 ctcactacca aatacactgg ttatgtaccg gccgatccag attctacaga aaaagagggt
     5101 tgtgaaaaat ggcccaaaac cgggtatcgc ttagaggacc aggttgattt tcatttactg
     5161 gctactatca atgagtttac tacctctggc ttgactctta ctgtcaccga tactttcacc
     5221 gataacccct ccgtctccgc cgaccatttt tatgtagagt ataagcaaga cccctggtac
     5281 atccggtttg gtagagtcca gctaaagagg ttcgatttag ggctggtttt gggtttgtat
     5341 cgaccggcag atccggaaag ctcttatgct cttgattttg atggcttcta tatagcctat
     5401 cagccggaag gaagtgaggt agcgtggaaa gcggtaggta gccctaacga tttctatatg
     5461 gtaagggcag aatgggaaga tacggctctg gctctgacct ggatgccaga aggtgatgag
     5521 ctatacagtc cagaaaactc cgacctcatc gtcagcgctg caatgaagac tgattttaat
     5581 caatcggatg tccggatgac tctagaggga gcctatggcg ttttatctga tacttatggc
     5641 ctggctggag agttagcttt caaagtcaag gaaaatgcta ctattactct ttcaggtcgc
     5701 tatattactg aaggttttac accggtatcg tctcagtata tagagtgggt aaatgatacc
     5761 tgtggaactg aacagcgtat cagccggtgg gatgccagta atcggatgat ctttactctt
     5821 ccggggaact ttcagctctc cgggcaaggg aaagaccagg aacaatggaa attgagtttt
     5881 ggttatacct atggagaaaa aaccactact ggagaaaaga tttcggatga agtgaacgcc
     5941 actttgactt atatccctgc tcaaccggta agtggagaaa gcgctactct acgagttaac
     6001 tactacatga tgaaagataa ttacggagtt tttggtggat accgaaatta ccccctggaa
     6061 attcaaaacg aaagtcaaga agcttatata agtgcaaatg ttcagtatgt tcctgattct
     6121 tcagaaataa ctgcggtggg agaactaagg tatcgctggc tggaagagaa aactaccctt
     6181 acttttcagg ggcgttatga ttcctctccc aagaaagagg acttttctac ctggggcttg
     6241 ctgactaatt tggaatggga aatggccaaa aatacccggc tcagcttagg ttatgagctg
     6301 aatacctggg atggtagcct tttcgcttat gatgacgata gagggattat tgataaagct
     6361 ggtactatga aagcagagct taaagtgagc ttctaaactt tcctacctct tagggagtaa
     6421 gttgatgggg aaatcctcga ttgggagagg taagggagtt ttatattttg cccttccatt
     6481 ccctcaggtt ataagggggc gttgtctttc ctgtctaaag aaggagggag gaggttgggg
     6541 ataccctctt ttatagaata gttgtgacca cacttccggc aatagagact tccttctaaa
     6601 acttctcccg aggcattttt ctcctttata gttagttcta aatttgtctt gcacacggga
     6661 caggctaaaa tatcaaccag ttccttcttc attatagtca ttcctcctta tatcgagaaa
     6721 gatctttccc gataatcatt tgtcttatat tatagttttg gttcttctat ttgtcacccc
     6781 ggcaactttc gagccgtttt tagcctgtcc ctgaatggtt ctgtcatccc cttgtccatc
     6841 attcctgagt gtttctatcg actataaggg ggtttacccc ctttataacc cccggaaaag
     6901 cgtaaaaacc taaaaacaaa cacggaaaat actactagtg ggaaaaatct ccaccagtag
     6961 tatttagatt cccgataaaa aatttcggga atgacaaaac tagggggaaa cctcgaattc
     7021 tttttccatc tcttttttcc tgtcatcgcg aggagcagga cagtctgcga cgtaaactaa
     7081 aaaagcaaaa tagataagaa aaaattggaa cgattacgct ggagaaaaga gcctcagcga
     7141 cgtggctatc tcacttttcg ctcctttttc cttctaggaa tagctctaca tactttttcc
     7201 ctcgactctc ttttatttcc ttctcccttg aatcctttcc ttttccctcg ccctttgctg
     7261 gagagcccca catattcctt ccctcgccct ccgagggaga gggttagggt gagggtgtct
     7321 tcttgccgtc attcctgaat gtttctat
//
LOCUS       DAOSVE010000085         7320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_5032_length_7320_cov_76.719202, whole genome shotgun sequence.
ACCESSION   DAOSVE010000085 DAOSVE010000000
VERSION     DAOSVE010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 7320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7320
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5032_length_7320_cov_76.719202"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(232..1680)
                     /locus_tag="PK016_07070"
     CDS             complement(232..1680)
                     /locus_tag="PK016_07070"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HXL02856.1"
                     /translation="MRQEYFFSFNFTEKDNKQHFPITFDVNEGAEEIVIYGAYYPETI
                     DEEKSKILVRECLRVYLNQGDVPLNVDTLSQQLLEKSLKEFIPIRNLLNFRIFDSLGN
                     FRGSGDSRISKGIPIKIGRSLSSLGCVTGDIYPGTWKLVVETHAVVNFCRLKLRIEIN
                     NKEETFGLPELAKPYQPVLNTCEQKRGWVKGDFHSHSNHSDGSLSIEELVQNAIDRKL
                     DFIFLTDHNKISGWETAQNEKFPLLPGIELSTFWGHFTAFGVTKYIDWSKIDPYKGIG
                     EVSDLVHNQGGLFCVAHPFTVGDPVCTGCRLIMDVDWNSVDALEIWAGTFNRRRHENT
                     EALKLWRNLLNQGYKIVGIGSTDVHSLQDINPDVPMTYVWLEELTLENILRSVKEGRV
                     YITREPEVEFKINTAKIGDTIDISDAPFILSYSISKDADLKIIYNGEETKSIKNTSYG
                     EIPLDLDEPGYIYLEFWKREELIALTNPIFIG"
     gene            2415..3353
                     /locus_tag="PK016_07075"
     CDS             2415..3353
                     /locus_tag="PK016_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXL02857.1"
                     /translation="MKCTRCKKEALVKIKRHNVKFCGDCFDIFFSRQVESAIKREKMF
                     SREEKVLIGVSGGKDSMSLWHYLQKAGYEVAAIHFDLGIEEHSQKSRQIVEEFASQHS
                     LPLEIIEVKKEIGFSIPEIKKRIPYRPVCSMCGLIKRYLLNFWSFKEHFSVYATGHNL
                     DDEAATLLGNVLHWQTDYLSHQSPTLPSPHPKMVKKVKPFYTLTEEEITIYAHLHNIP
                     FLEERCPLSERAKSWDYKEALNFLEDKSPGTKHMFWLGFLETGQKYFRQEEPPLYLRE
                     CSICSMPTTQEVCSFCRLMQKVKEREETHLDYNGSK"
     gene            3340..4527
                     /locus_tag="PK016_07080"
     CDS             3340..4527
                     /locus_tag="PK016_07080"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HXL02858.1"
                     /translation="MAVNSGGSSIKYELFSIEEDKERSLARGSVKRLYRSDSFWEGEK
                     EGTKTKKEVPHLDHEKGLRLIIDVLTQEGPLRNIQEIEAVGIKLINGGAKVTQTSFID
                     SGVIQALQDLSGVTSVHNPPSLLAIEMFQKILPQTPLVGVFETNFHLSIPLPYRTYGL
                     PWELSQKYGLEKLGFHGNSHRYIAERIIALTSQSQKVISCHLGSGCSVCAIKEGKSLD
                     VSSGFTPQSGTIMSTRPGDFDPQIITYLEEKGGFSYQEINRILTKESGLLGISGLSGE
                     MWELEELSQKGEERAKLAIEVFIYQVKKYIGAFTALLNGLDSLVFTGGIGENDSFIRE
                     KICGNLSYLGINFDREKNQEIKGKEGKISSSTSPVEVWIIPTNEELMVARETARIVKE
                     RRK"
     gene            4529..4963
                     /locus_tag="PK016_07085"
     CDS             4529..4963
                     /locus_tag="PK016_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HXL02859.1"
                     /translation="MTSGEYHDQGFNCCESILQGLGDYLGVKSDLIPSIATGFGGGIG
                     HTGGICGAITGAVMALGIKYGRKDPRDKEKRDWFYKMVENFLQDAEKELGSLSCRDLI
                     GLTLNTDETMKIYREKNLRRKCHEIISQVEKLAQAYLSLSYS"
     gene            complement(5396..6898)
                     /locus_tag="PK016_07090"
     CDS             complement(5396..6898)
                     /locus_tag="PK016_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HXL02860.1"
                     /translation="MERYSLVLDCGATSLRAVAVSEKGEILGLSSFPNAPSPQEGEKD
                     FLIWDVEEIWRKFTLAIRQVISEVGREILAVTVTTFGADGAPLRRDGTLTYPVISWQC
                     QRTAPQAKEITNFLSPREIFRITGYQIIPFNTIFKLLWLRENAPCSLDEADTFLMMPG
                     ILSFKLCGEMSMDFTSASTTMMLDIERKCWSQDLLSLVGLSPSFFPPLQCPGEVIGRV
                     HSKTSEETGIPRGTPVIAAGHDTQFAIVGSLASPEELVLSSGTWEISALRLPYYRDSS
                     SFFEQGGLTELDAEENLWNPQMLMMAGGVVEWVRRNFFSDIKDEKNIYDVMIESARSI
                     SPGAGGIVVIPAFMPSGPTKPYATQGTILGLELTSDRGQVFRATLEGLSFQLKEAVLA
                     LEEAFGYNPQRVRVVGGGSKNKLWNQIRADVLNLPVVTTSCQEATVLGASLFSQVGAG
                     IVKNLEEAKSRVKMETTVWEPSPQKEIYEELFSQYTKLPPLLAPFYRERS"
BASE COUNT         2352 a         1484 c         1410 g         2074 t
ORIGIN      
        1 aaagtgattg tcaacagaaa atggaaaaaa attaagggct gaaacgatta gaaatgttta
       61 gtttataaat gtttaaaacg acaaggataa gttggttggt aaagctaata aataattttc
      121 aattttttat ttagaaccta aaagtgtact tattgaaaga agaataaact ccttgggagt
      181 ttgaaaaata ataatactgc gaaccccaag gagaacgtat cgcatgacct gttagccaat
      241 aaatattgga ttagtcaagg ctataagctc ttctctcttc cagaattcca ggtatatata
      301 tcctggctca tcaaggtcga gaggtatttc tccgtagctt gtgttcttga tagacttagt
      361 ttcttctcca ttatagatta ttttaaggtc agcatcttta gagattgaat aacttaagat
      421 aaaaggagca tcagaaatat ctatagtgtc tccaattttt gcagtgttaa tcttaaactc
      481 cacctctggc tccctggtaa tatatactct tccttctttt acggaacgta gtatattttc
      541 taaagttagt tcctcaagcc agacatatgt catgggcaca tctggattaa tatcctgtaa
      601 tgaatgaacg tccgttgagc ctattcccac tattttatag ccctgattca ggagattccg
      661 ccagagcttt agagcctctg tattttcgtg tcttcttcta ttgaatgttc ctgcccatat
      721 ttctaaagca tctacagaat tccaatctac gtccattatt aacctgcagc ctgtacatac
      781 cggatcacct acggtgaatg gatgagcaac acagaagagc cctccctgat tatgaactaa
      841 atcagaaacc tcccctatac ctttgtaggg gtctatcttg ctccagtcta tgtatttggt
      901 tacgccaaaa gcagtaaagt gcccccagaa ggtagagagt tcgataccag gtaataaagg
      961 gaatttttca ttctgggcag tctcccaacc ggaaatctta ttgtggtctg taagaaagat
     1021 aaaatcaagt tttctatcaa tagcattctg aaccaattct tctatagaca agctcccgtc
     1081 actgtggtta gagtgagaat gaaagtctcc tttaacccat cctctcttct gttcacaggt
     1141 attcaaaaca ggctgatatg gcttggccag ctctggaagg ccgaaggttt cttctttatt
     1201 gtttatttca atcctcaatt tcagtctgca gaaattcaca acagcatggg tttcaaccac
     1261 taatttccag gttccgggat agatgtcacc agtaacacac cctaaagagc ttaaacttct
     1321 acctatcttt ataggtattc ctttagaaat tctactatca ccacttcctc taaaattacc
     1381 aagggaatca aatattctaa aattcagaag atttcggata gggataaact ctttcaggct
     1441 tttttctaaa agctgctgag ataaggtgtc aacgttcaga ggcacatccc cctggttcag
     1501 atagactctt aagcattccc tgactaatat tttagatttc tcttcatcaa tggtttcagg
     1561 ataatatgct ccgtaaatta ctatttcctc tgcaccttca tttacatcaa aggttatagg
     1621 gaagtgttgc ttattatctt tttctgtaaa attgaaacta aagaaatatt cttgcctcat
     1681 ttctaccccg ctcaggatga atccgcctca ttatagtttt tatagttaac cgcaaaatcc
     1741 attacaaata ctataaaggg gagtgcccct ttataatccc ccaaaaaaca aaaaaataca
     1801 aaaagaaaaa gactactgag gaaagaaaaa cgctttcctc agtataaggg gggaaagaat
     1861 tcccccttat gaatcccctt tctttttctt ccctcgcctt caatggaaga accccacata
     1921 ttccttccct cgccctcgaa gggagagggt tagggtgagg gtgaaagcta taagaaagct
     1981 ttgccccttt ataatccccc aaaagcaagg aacaaagaaa caaaaggcta ctggaaatat
     2041 tatatagtat cccgcagtac atagcgacct tgagtttttg actttcgata taatttactt
     2101 tttaaagttt ttaacatttt aatcagtttt gcgggatact atatatgaaa ctccttcaag
     2161 catttttggg gctttataat aaagtgtgca gaaaaaatat ctaaaaaagt atgaagtaaa
     2221 caaaagggtc taaaatagca gaaatctgta gaaagcaaga gatatttaag ctttgcatta
     2281 cgaatgatag gataaacttc ctgatacaga tataaatcca ggaagaactc ctgacctcta
     2341 tgcttttgag gagtaaaata tgtcttgaca aaccagtcgt gctacattta aatctaaaat
     2401 aaaagggaga caaaatgaag tgtactcgat gtaaaaaaga agctctggtc aagataaaaa
     2461 gacataatgt taagttttgt ggggattgtt ttgacatttt tttctcccgc caggtagaat
     2521 cagccataaa aagagaaaaa atgttttctc gggaagagaa agtgctaatc ggggtatcag
     2581 ggggcaaaga cagcatgtct ctgtggcact acctccaaaa agcaggatat gaggtagcag
     2641 ctattcattt cgatttaggg atagaagagc actctcagaa gtctcgccag atagtagaag
     2701 aattcgcttc tcaacactct cttcccttag aaatcataga ggttaaaaaa gagatagggt
     2761 tttctattcc ggagattaaa aagcggatcc cctatcgtcc tgtctgttct atgtgtggcc
     2821 tcattaaaag gtatctttta aacttctggt ctttcaagga acatttttca gtttatgcca
     2881 ccggtcataa tttagatgat gaagcagcca cccttttagg taatgtttta cattggcaaa
     2941 cagattacct atctcatcag tctcctactc ttcccagccc tcaccctaaa atggtgaaaa
     3001 aagtaaagcc tttttacact ctcaccgaag aggaaataac gatttatgct cacttgcata
     3061 acatcccttt cttagaagaa agatgcccac tctcagaaag agccaagagc tgggattaca
     3121 aagaagcgtt gaacttcctg gaagacaaat ctcccggcac caagcatatg ttctggttag
     3181 gatttttaga aaccgggcaa aaatattttc gacaggaaga gccgcctctt tatttgcgag
     3241 agtgctctat ttgtagtatg cccaccactc aagaggtttg ttctttctgt cggctaatgc
     3301 aaaaagtaaa agaaagggag gaaacgcatc ttgattataa tggcagtaaa tagtggtggc
     3361 tcatctatca agtatgaact cttttccata gaagaagata aagaaaggtc tctcgcccgg
     3421 gggagcgtaa aaagattata ccgttctgat tccttctggg aaggagagaa agaaggaaca
     3481 aagactaaaa aagaagttcc ccacctcgac cacgaaaaag gtctgcgtct gataatcgat
     3541 gtcttaactc aggaaggtcc tctacgcaat atccaggaga tcgaagcagt gggcataaag
     3601 ctcatcaacg ggggagcaaa ggttactcaa acctctttta tcgactccgg cgttatccag
     3661 gctctccagg acctctccgg ggtaacttca gtccacaatc ctccttctct tttagccata
     3721 gaaatgtttc aaaagatcct tcctcagact cctttagtag gagtttttga aaccaatttt
     3781 cacctttcta tacccctacc ctaccgcact tatggtctac cctgggaatt aagtcagaag
     3841 tacggtttag aaaaattagg atttcatggt aattcccacc gctacatagc tgagaggatt
     3901 atcgctctta cttctcaaag ccaaaaagta ataagctgtc acttaggaag tggatgtagt
     3961 gtctgtgcca taaaagaggg gaagtcttta gatgtctcga gtggctttac tcctcaaagc
     4021 ggaactatta tgtccactcg cccgggagac tttgaccccc agataatcac ttatctggaa
     4081 gaaaaaggag ggttttccta tcaggaaatc aaccgtattc taactaaaga atccggactt
     4141 ttaggaatat cagggttaag tggagaaatg tgggaactgg aggagttatc ccaaaaagga
     4201 gaagagagag caaaattagc gatagaagta tttatctatc aggttaaaaa atatatcgga
     4261 gccttcactg ccctcttgaa cggtttagat agtctggttt ttactggagg aataggagaa
     4321 aacgatagtt tcatccggga aaaaatttgt ggcaatcttt cctatttagg aataaatttt
     4381 gatcgggaga aaaatcaaga aatcaaagga aaagaaggta aaataagctc ttctacttct
     4441 ccggttgagg tatggattat acctactaac gaagaactaa tggtcgcccg agagacggcc
     4501 aggatagtta aagaaaggag aaaataaaat gacttccggt gaatatcatg accagggatt
     4561 taactgttgt gaatctattc tccaaggtct aggagattat ttaggagtaa agagcgattt
     4621 aattccttcc attgctaccg gctttggagg aggaattgga cacacgggag ggatttgtgg
     4681 agcaataacc ggagcagtga tggctctggg aataaaatat ggaagaaaag acccccggga
     4741 caaggaaaaa agggactggt tctacaaaat ggtagaaaat ttcctgcaag atgcagaaaa
     4801 agagttaggt agcctgagct gtcgagacct cattggtctc accttaaata ctgatgaaac
     4861 gatgaaaatc taccgggaga aaaatctgcg cagaaaatgc catgagataa ttagccaggt
     4921 agaaaaattg gcccaggctt atctatctct cagctatagt taatcgcaaa agacagcctg
     4981 cggcgcaaga ctaaaaaaaa taaaaaaaca gaaaaaccac tgaggaagag aaacatcttc
     5041 ctcaagctct cgataacagt taatcacagt gagattttgt ggttaactat gacatcatct
     5101 tgaacttata tgactaggtt tacagtgttt agatgatata gtttaatcta ccaggtagtt
     5161 agtatttatt cctttttgtc aatttgattc atctccaaaa cctcttttgg tgttgagatc
     5221 agatagatat gggtaatggt gagataaccc ccaaaaggca aaaaaataaa tacaaaaaga
     5281 aaagaccacc gtagaaatta tagtatccct aagtactttg ctatctttta acttcctgtt
     5341 gaatttgttt ttttaaagtt tttaacagtt tacagatttt gcgggatact ataatttagc
     5401 ttctctcccg ataaaaagga gctaaaagag gaggaagttt tgtatattgg gaaaaaagtt
     5461 cctcatatat ttctttctga ggagaaggct cccacactgt ggtttccatt ttcacccggc
     5521 ttttagcttc ctccaggttt tttactattc ctgcccccac ctgagaaaaa agagaggctc
     5581 ctaaaacagt agcttcttgg caagatgtgg taaccacggg aagatttaat acatcagctc
     5641 tgatctgatt ccatagttta tttttggacc ctcctcctac cactcgcact ctttggggat
     5701 tatagccaaa agcttcctct aaagctaaaa cagcttcttt caattgaaaa gataaacctt
     5761 ccagagtagc gcgaaagact tgtcctcggt cagaggttaa ctccagccct aaaatagtac
     5821 cttgagtggc ataaggttta gtaggaccag aaggcataaa agcaggaatt accactattc
     5881 ctcctgcacc aggggaaata gagcgggcag actctatcat tacatcatag atatttttct
     5941 catctttgat gtcagagaaa aagttccttc tcacccattc tactactcct ccagccatca
     6001 tgagcatctg ggggttccac aaattctctt cagcatctaa ttctgttaga cctccctgct
     6061 caaaaaaaga agaagaatct cgataatagg gaaggcgcag cgcagaaatc tcccaggtgc
     6121 cagaacttaa taccaattcc tctggagaag ctaaagaacc cactatggca aactgggtgt
     6181 catggccggc tgctataact ggtgtacccc gggggattcc ggtttcctca cttgtcttag
     6241 aatgaaccct tccaataacc tcaccaggac actgaagagg agggaaaaag gaaggggata
     6301 aacctactag agaaagtaag tcctgagacc agcattttct ctctatatct aacatcatag
     6361 tggtagaggc agaggtgaaa tccatactca tttctccgca aagtttgaaa ctcaagatac
     6421 caggcatcat caaaaaagtg tcagcttcat ctaaagagca aggagcattt tctcgcaacc
     6481 agagaagttt gaatatagta ttgaaaggta ttatctgata tccagtaata cgaaaaatct
     6541 cccgaggact aagaaaattg gtgatttctt tagcctgagg agcagtcctc tggcactgcc
     6601 aggagataac cggataggta agcgtcccat ccctgcgaag aggtgctcca tccgctccaa
     6661 aagtagtaac agtaacagcc aaaatttccc ttcctacttc actaataacc tgccggatgg
     6721 ctaaagtaaa ctttctccag atttcctcca catcccagat gaggaagtct ttttcccctt
     6781 cctgaggaga aggagcatta ggaaaagaag aaagacctaa tatttctcct ttttcactaa
     6841 ctgccacggc tctaaggctg gtagctccac aatctaaaac taaactatat ctttccatga
     6901 tctccccccc tgaaaaaaca tatattccgg taataattaa tccttagaga tcagtgcttc
     6961 aacaaaagta ttacgcacct gactaaagac aaattctccc tgaaacaccg gacctcttgc
     7021 taaattgttt ttataagata gtccacatct aaaatcgtag tgcatggtgg actgagaacg
     7081 tcccttccat ctccaatgaa gccaggaata aaagataaaa ctccaaaaag ataaaaactt
     7141 tctctctggg acttcaataa ttaagattaa tctcccgata aaaccactct tagataggat
     7201 ttagctattc acaaattacg aatgcgctcc ccaatatctt ttgattgaaa ttattctatc
     7261 aattctgtca atgttcttct attctggtga agaatcattt cttttaaggt ataatggtac
//
LOCUS       DAOSVE010000086         7292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_5052_length_7292_cov_71.421584, whole genome shotgun sequence.
ACCESSION   DAOSVE010000086 DAOSVE010000000
VERSION     DAOSVE010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 7292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7292
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5052_length_7292_cov_71.421584"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..810
                     /locus_tag="PK016_07095"
     CDS             169..810
                     /locus_tag="PK016_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXL02861.1"
                     /translation="MRKLKIRWFGHSCFLVTSGDIKIVFDPFDNTVGYPLPQVEANLV
                     VVSHDHFDHNNVSVVQGHPEIIKKPGVYEKDGIKIEIFSTFHDEVGGKKRGKNNVARL
                     ELEEMSLIHTGDLGIIPPDEELSSWKPVDILLLPVGGVFTIDAREAQKLVPKLSPRIV
                     IPMHYRTKYTQMEISTVEPFLHGFKEIKRLDTSEIEVTQKNLPAGMEVWVLGI"
     gene            965..1240
                     /locus_tag="PK016_07100"
     CDS             965..1240
                     /locus_tag="PK016_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HXL02862.1"
                     /translation="MVTISVCVGSSCHLKGAYEIIETCEELIEKLKLKDQIELKGNFC
                     LGRCTEEGVTVMVDDEILSGVSPQNFREIFQEKILKKLEEGGANGSN"
     gene            1227..2945
                     /locus_tag="PK016_07105"
     CDS             1227..2945
                     /locus_tag="PK016_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HXL02863.1"
                     /translation="MGVISTIEARCRDCYKCIRNCPVKAIRVSLGHAEVVDERCIKDG
                     RCVLVCPQDAKKIQNDLPRVQELLRGEDPVVVSLAPSFVAAFSEGTEGQIVAALKKLG
                     FQEVRETAEGAEWVAKEHTRLLSEGRKNIISSSCPAINALIYKYYPQYVSYLAPVVSP
                     MIAHARLIRQEYGKVKVVFIGPCIAKKAEVEEEEVKGEVDAVLTFEELKTWFQEENIS
                     LSVLEEEEMGLSSVEWGRAFPVEGGLLKTASLDTGILSKEVMVITGIDRCRQFLDDLE
                     NARSSFEMVEMMSCEGGCIDGPVLQSLLSPYERRKKVIDYTEKAKMVYNPPPEKKFIS
                     LPSFSRDFVPHPVPRPEVPEEEIKRILSLTGKFSPQDELNCGACGYNTCREKAQAVYQ
                     GMAEAEMCIPFMRAKAESFSSFLIAVTPNGIVLVDENLRIVDVNPALRRMFGLTGKLV
                     VGSKLDQFIDPSPFVEVLRTKRVVTKEVSYPQYQNLYVQLSVFYLEKSQLLMGVFVDL
                     TKEREREALMEQIREKTLEKAQQVINNQMTVAQEIASLLGETTAETKVLLNKLMKILR
                     GESVEE"
     gene            2942..4096
                     /locus_tag="PK016_07110"
     CDS             2942..4096
                     /locus_tag="PK016_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HXL02864.1"
                     /translation="MKDLFVEFAYAQLPKWGEELCGDSVSYDKGNNSTILSWSDGLGS
                     GVKANILSTLTSKIIVSMLKRESTLEEVIATLAETLPVCKVRKIAYSTFSILQILNDG
                     ETYVAEFDNPPLFWLRNGRLMHLDRKEREIGKRVVLESLIRLQKNDWIVGVTDGVVHA
                     GIGGLWNLGWRWEKIASFLENTVNDQLDALTLANKVIATTKRLYQDRLGDDATCIVAK
                     IRLPRYLIVWAGPPEDPTLDEYIVTKVMEFPGKKAVCGGTTGNILSRVLGKEIKVDLT
                     SIQKDIPPVGIMEGVDLLTEGILTLATVLRQLRKGVKLEEVMYRQDGAGRLLSLLLEA
                     DEITFIGGRAINPAHQNPKLSGELSLKARVLEDIVSELQKKGKIVTLEYY"
     gene            4130..4645
                     /gene="nuoE"
                     /locus_tag="PK016_07115"
     CDS             4130..4645
                     /gene="nuoE"
                     /locus_tag="PK016_07115"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010865339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HXL02865.1"
                     /translation="METEKETLVLGRQFEKVNAILEKYSHSVDNLIAILQEIQSEYRY
                     LPEEILTYVATALQVSPAQVYGVATFYAQFSLEPKGEHIIRVCDGTACHVKGSTQVLK
                     AVRENLGLREGENTTSDLLFTVETVACIGACALAPALMVDEEVYGQMDEQKVQQLIDR
                     LLGEGEKVDAV"
     gene            4635..6527
                     /locus_tag="PK016_07120"
     CDS             4635..6527
                     /locus_tag="PK016_07120"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HXL02866.1"
                     /translation="MRFENREQLEQYIAELKSKNGKRKVLICGGLGCLAKGAEEVFNT
                     FREVVEKEKVAAEVEKLEESPHLVKTGCMGLCEAGPLVRIEPEGILYLRVKPEDVEEI
                     VEKTLKNGEVVERLVYQEKGDGEVHYWPHLKDVPFYRKQVKITLRNCGSIDPQNMNEY
                     LARDGYLALARVLSEKTPDEVIEEITKSGLRGRGGGGFPTGRKWQFARVVKGDKKYVI
                     CNGDEGDPGAFMDRSVMEGDPHSILEGMAIAGYAIGAQEGYIYVRAEYPLAVKHLRKA
                     IEDAEKVGLLGNNILGSNFSFRITIREGAGAFVCGEETALISSIEGKRGMPNPRPPYP
                     AYRGLWGKPTIINNVETMANVPIIMLRGAEWFRSFGTANSAGTKTFALAGKIKNTGLV
                     EVPMGITLRDLIFDIGGGIQNDKKFKAVQIGGPSGGCLTERHLDLPIDYDSLSGAGAI
                     MGSGGLVVLDEDTCIPEVARFFMNFTQNESCGKCVPCREGTKHMLGLLQKVVKGEAST
                     SDLELLEETAHVVRDASLCGLGKTAPNPVLTTLRYFRDEYLDHVVYKVCPAHVCQAMK
                     EYRIDPEKCRSCAKCARVCPVQCISGRPRVPYVIDQEKCTKCGACFEACPFDSITVEW
                     RKKEDE"
     gene            6520..>7292
                     /locus_tag="PK016_07125"
     CDS             6520..>7292
                     /locus_tag="PK016_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HXL02867.1"
                     /translation="MNEKIAKKAVVVDGQEITLNGEKNILEVAKKANIEIPTFCYHSD
                     LSVYGACRMCLVEVEGRGLMPACSTPPEPGMVIKTTTSRVLQARRMMVEFYLANHERD
                     CTVCERNLNCRLQAIAASLGIDEVRFGERKEKLPKDTSTFSLVRDPNRCILCGDCVRA
                     CKEIENIGIYDFTQRGSRTKVEPAFGKKLSEVECVYCGQCSVRCPTGALVVRSELDNA
                     WKAVLNPDLMVVVQIAPAVRVAIGEAFGLPPGEITTGKIV"
BASE COUNT         2204 a         1207 c         1831 g         2050 t
ORIGIN      
        1 taatggattt tgcggttaac tataaaaggc caaataattt aggaacgggc aatcttagtg
       61 gcttctggac atcctgggtc ggcataaaga ttttcaggaa tgagaaaaaa agggaaagaa
      121 agtataaggg ttttggttat ctattttata gtaaaatagg tgtaggaggt gaggaaattg
      181 aaaattcgtt ggtttgggca ttcttgcttt ctggtgacga gcggagatat caaaatagtc
      241 ttcgaccctt ttgataacac tgtaggttat cctcttcccc aggtggaagc aaatttagta
      301 gtggtgagcc atgaccactt tgaccacaat aatgtttctg tggtgcaggg gcaccctgag
      361 ataattaaaa aaccaggagt ttatgaaaaa gacgggatta aaatagaaat tttttccact
      421 tttcatgatg aggtgggagg gaaaaaacga ggcaaaaata atgttgcccg attggaatta
      481 gaagagatgt ctctcattca tactggagat ttaggaataa tacctccaga tgaggagctc
      541 tcttcctgga aaccagtaga tattttatta cttcccgtgg gtggagtttt cactatcgat
      601 gctcgagagg ctcaaaaatt ggttcctaaa ctttctcctc gtatcgtgat accgatgcat
      661 tatcgaacta aatataccca gatggaaatc agtaccgtag aacccttttt acacggtttt
      721 aaggaaatta aaaggttaga tacttctgag atagaagtaa cccagaagaa tctccctgcc
      781 ggtatggaag tgtgggtttt gggaatctaa atttatgtta taataaccgg ctaaaacgta
      841 atttgggtaa tttatcaaga agaacataaa aattgaggga ggtgatttca ctccctctgt
      901 ttttatgttt aatatagtgt tcttggcaat ctagatgaag tagtattttt caggagaggt
      961 aaaaatggtt actatatcag tttgtgtagg tagttcctgc catctaaagg gagcttatga
     1021 gattattgaa acttgtgaag agttgattga aaaattgaag cttaaagatc aaatagaatt
     1081 aaaaggtaac ttctgtctgg gaagatgcac ggaagaggga gttaccgtaa tggtggatga
     1141 tgaaatactc agtggagttt ctcctcaaaa tttcagggaa attttccagg agaagatttt
     1201 gaaaaagtta gaagaaggtg gtgctaatgg gagtaattag taccattgag gcaagatgcc
     1261 gggattgcta taaatgtata cgcaattgtc cggtaaaggc cattcgagtg agtttaggac
     1321 acgctgaagt tgtagatgaa aggtgcataa aagatggacg gtgtgttttg gtttgtcctc
     1381 aagatgccaa aaaaattcaa aatgacctcc ctcgagttca agagctcctc cgaggagaag
     1441 acccggtagt ggtcagcttg gctccttctt ttgtagccgc attttcggaa ggaacggaag
     1501 gacagatagt ggcagctcta aaaaaattgg gttttcaaga agtgcgagaa acagcggaag
     1561 gagcggagtg ggtagctaaa gaacataccc ggctcttgag tgaggggaga aaaaatatca
     1621 tttctagttc ttgtccggct atcaatgctt tgatttacaa atattatcct caatatgttt
     1681 cttatcttgc tcccgttgtc tctcctatga ttgctcatgc tcgtttaatt cgtcaggagt
     1741 atggaaaagt aaaagtggtc tttatcggcc cttgcattgc caaaaaagca gaagttgaag
     1801 aggaagaagt gaaaggagaa gtagatgcag ttttaacttt tgaagaatta aaaacctggt
     1861 ttcaggaaga gaatatctct ctttctgtat tagaggaaga agaaatgggt ttatcttcag
     1921 tggagtgggg aagagctttc cccgtggaag gaggactcct aaaaactgcc tctttagata
     1981 ccgggatttt atccaaagaa gttatggtga taaccgggat tgatcgctgc cgccaatttt
     2041 tagatgattt agaaaatgcc agaagcagct ttgaaatggt ggaaatgatg tcctgtgagg
     2101 gaggttgcat cgatggaccg gtgctccaat ctttgctctc tccttacgaa cgacgaaaaa
     2161 aagtcattga ctacaccgaa aaagctaaga tggtttataa tcctccacca gagaaaaaat
     2221 ttatttctct tcccagtttc agtcgagact ttgtacctca tcctgtgcct cgaccagaag
     2281 ttccagaaga agagataaaa agaattttat ctctcaccgg caaattttct cctcaagatg
     2341 agttgaattg cggtgcttgt ggatataaca cctgtcgaga aaaagctcaa gcagtttatc
     2401 agggtatggc agaagcggag atgtgcattc cttttatgcg agccaaagcg gaatcttttt
     2461 ccagcttttt gattgccgta actcccaatg gtattgtgct ggtcgatgaa aatctgcgca
     2521 tcgtagatgt taacccggct ctccgcagga tgtttggcct gacagggaaa ttagtggtgg
     2581 gaagtaagtt agaccagttt atcgatcctt ctccttttgt agaagtgttg cgtaccaaaa
     2641 gggtagttac taaagaggta tcttatcctc aataccagaa cctttacgtt caattatccg
     2701 ttttttatct ggagaaatct cagcttctca tgggagtatt tgtggatttg actaaagaaa
     2761 gggaaagaga agctttaatg gagcaaatta gagagaaaac tctggaaaaa gctcaacagg
     2821 ttattaataa ccagatgacg gtagctcaag aaattgccag tttactggga gaaactactg
     2881 ctgagactaa agtgcttctg aataagttga tgaaaatttt acggggagaa tcagtagagg
     2941 aatgaaagac ctctttgtag agttcgctta tgctcagctt cccaagtggg gagaagagct
     3001 ttgcggagat agcgtaagtt atgataaagg aaataattcc accatattat cctggtctga
     3061 tggcttaggt agtggagtaa aggccaatat cctttccacc ctgacttcga aaattattgt
     3121 ttctatgctg aaaagagaat ctactttaga agaagtgata gccactttag cagaaacttt
     3181 accggtatgc aaggtaagaa aaattgctta cagtactttt tccatcttgc agattctgaa
     3241 tgatggagaa acttatgtag cagaatttga taatccccct cttttttggt tgcgcaatgg
     3301 aaggttgatg catctggata gaaaggaaag agaaataggt aaaagagtag ttttagaatc
     3361 tttaatcagg ttacagaaaa atgattggat tgtaggagta actgatggag tagttcacgc
     3421 cgggattgga ggattatgga atttaggatg gaggtgggaa aaaattgcct cttttttaga
     3481 aaatactgtt aacgaccaac tggatgcttt aactctggcc aataaagtaa tagcgactac
     3541 caaaaggctt tatcaggata gattaggtga tgatgctacc tgtatagtag ctaaaatacg
     3601 ccttcctcgt tatttaatag tgtgggctgg ccctcctgaa gaccccacct tagatgaata
     3661 tatagttaca aaagtaatgg aatttccagg taaaaaagca gtttgtggtg gcactacagg
     3721 aaacattttg tcccgggttc tgggcaaaga aataaaagta gatttaactt ctatacaaaa
     3781 agatattcct ccggtaggga taatggaagg agtggattta ctcaccgaag ggattttaac
     3841 tctggccacg gtccttcgtc agctacggaa gggagtgaag ctggaagagg ttatgtatcg
     3901 acaggacgga gccggtcgct tactttcact tttactggaa gcagatgaga ttacctttat
     3961 agggggaaga gctataaatc ctgctcatca aaatcccaaa ttatctggag agctttcact
     4021 aaaagctcga gttttagaag atatagtcag tgagttgcaa aagaaaggga aaatagttac
     4081 tctggaatat tactaaaaaa ctaaggttat ctatagagga ggtagaacag tggaaacaga
     4141 aaaggagact ttagttctcg gtcgtcagtt tgaaaaagtt aacgctattc tggagaaata
     4201 ctctcatagt gtggataatt tgatcgccat attacaggaa attcagtccg aatatcgtta
     4261 tctaccggaa gagattctaa cctatgttgc tacggctctg caggtttctc cagctcaagt
     4321 ttatggggtg gctacttttt atgctcagtt ttctttagag cccaaaggtg aacatatcat
     4381 cagagtttgc gatggtaccg catgtcatgt aaagggttct actcaagttc taaaggcagt
     4441 tcgagaaaat ttaggactga gagagggaga aaacaccacc tcagatttac ttttcaccgt
     4501 ggagacggtg gcctgtatcg gagcttgcgc tctggctccg gctcttatgg tggatgaaga
     4561 agtttatggc caaatggatg aacaaaaagt gcagcagctt atcgatcgcc tgttagggga
     4621 aggagagaaa gtagatgcgg tttgagaatc gggagcagtt agaacaatac atcgctgaat
     4681 taaaaagtaa aaatggaaaa aggaaagtcc tgatttgtgg aggcctggga tgtttggcta
     4741 aaggagcaga agaagtattt aatactttcc gagaagtggt agaaaaagaa aaggtagccg
     4801 cagaggttga aaaattagaa gaaagccccc acttagtgaa aactggttgt atggggctct
     4861 gtgaagctgg acctctcgtg agaatagaac cagagggaat tctgtatctg agggtgaaac
     4921 ccgaagatgt agaggaaata gtggagaaga ctcttaaaaa tggagaagtg gtagagagac
     4981 tggtttatca ggaaaaagga gatggagaag ttcattactg gcctcatctt aaggatgtcc
     5041 ctttttaccg aaagcaggtt aaaattactc ttcgcaactg tggctctatt gatccccaga
     5101 acatgaatga atatttagct cgagatggtt acttagcttt agctcgagtc ctaagcgaaa
     5161 aaactcctga tgaggttatc gaagaaatca ctaaatcagg tttgcgagga agagggggag
     5221 gaggtttccc cactggaaga aaatggcaat ttgctcgagt ggttaaagga gataaaaaat
     5281 atgtaatctg taatggcgat gaaggcgacc cgggcgcttt tatggatagg agcgtgatgg
     5341 aaggagaccc tcattctatt ttagaaggta tggctattgc cggttatgct ataggagcgc
     5401 aagaaggata tatttatgtc cgggctgagt atcctttggc agtgaagcac ctgaggaaag
     5461 ctatagaaga tgcggaaaaa gtcgggcttt taggaaataa cattttaggt agtaattttt
     5521 ctttccggat tacaatccgg gaaggggcag gagcttttgt ctgcggagaa gaaaccgctt
     5581 tgatttcttc tattgaaggg aaaagaggaa tgcccaatcc tcgtcctcct tatcctgctt
     5641 atcgaggtct atggggtaaa cctactataa tcaataatgt agaaactatg gctaatgtac
     5701 cgataatcat gcttcgggga gcagagtggt ttcgttcttt tgggacggca aacagtgcag
     5761 gaactaaaac ttttgctcta gccggcaaaa ttaaaaatac cgggttagtg gaagtcccta
     5821 tggggattac cttgcgagac ttgatttttg atattggggg aggaatacag aacgataaaa
     5881 agtttaaagc ggtgcaaatt ggaggaccat cgggggggtg tttgactgaa agacaccttg
     5941 atttacccat cgattacgat tccctgtccg gggcaggagc cattatgggc tctggcggat
     6001 tggtggtact agatgaggat acctgtattc cagaagtagc tcgctttttt atgaacttta
     6061 cccagaatga atcttgtggt aaatgtgttc cctgccgaga aggaactaaa catatgttag
     6121 gtctgttgca aaaagtagtg aagggagagg ccagcacttc agatctggaa cttttagaag
     6181 aaaccgccca tgtggtgaga gatgcttctt tatgtggttt aggtaaaact gctcctaatc
     6241 ctgttcttac cactcttcgc tatttccggg atgagtattt agaccatgtg gtctataagg
     6301 tttgccctgc tcacgtttgc caggcgatga aggaatatcg gattgacccg gaaaaatgtc
     6361 gttcttgtgc taagtgtgct cgggtttgtc cagtgcaatg tatttctggt cgacctcgag
     6421 tgccttatgt tatagaccag gaaaaatgta ctaaatgtgg ggcttgtttt gaagcctgtc
     6481 cctttgattc cataactgtg gagtggagga aaaaagaaga tgaatgaaaa aatagctaaa
     6541 aaagcagtag tggtagatgg gcaagaaatt accttaaacg gtgagaagaa tattctggaa
     6601 gtggccaaga aagccaatat tgagattcct accttctgtt accattctga cttgagcgtt
     6661 tatggagcct gccggatgtg tcttgtggag gtggaaggac gaggtctgat gcctgcttgc
     6721 tccactcccc ctgagccagg aatggtgata aagactacca cttctcgggt tcttcaagcc
     6781 cggcgtatga tggtagaatt ttatctggct aatcatgaga gagattgtac agtatgtgag
     6841 agaaatctca actgccgcct gcaagccatc gctgcttcct tgggaataga cgaagttcgg
     6901 tttggagaga gaaaggagaa gttgcctaag gatacttcta cattttcctt agttcgagac
     6961 cctaatcgtt gtattctatg cggagattgt gtacgagctt gcaaagaaat agaaaacatt
     7021 ggaatttatg attttactca gcgaggttct cgaactaaag tagaacccgc ttttggaaaa
     7081 aagttgtcag aagtagagtg tgtttattgc ggccaatgct ctgttcgttg tcctactggt
     7141 gctttagtag taagatcgga gttggataac gcctggaagg cagtccttaa tcctgattta
     7201 atggtagtgg tccaaattgc tccggcagta cgagtagcca taggagaagc ttttggtctt
     7261 cctccgggag aaatcactac cggtaaaatt gt
//
LOCUS       DAOSVE010000087         7270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_5071_length_7270_cov_69.133195, whole genome shotgun sequence.
ACCESSION   DAOSVE010000087 DAOSVE010000000
VERSION     DAOSVE010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 7270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7270
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5071_length_7270_cov_69.133195"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            356..937
                     /locus_tag="PK016_07130"
     CDS             356..937
                     /locus_tag="PK016_07130"
                     /inference="COORDINATES: protein motif:HMM:NF016216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HXL02868.1"
                     /translation="MEDLLNIWREFSVIGALRTSSWKDINDEELSWCPVFFFLEGNVF
                     TLREALKILPPDLHLFLHLDLFGGIASDESGLLFMRETFPQIRGIISTRARTLNLARK
                     AGFLTIFRLFLIDSESLRTGLRIAQEISPDALEILPGIIFPQVRNSIPVEDLPPLICG
                     GFIRKKEEVEEIISRGAVAVSTSCRELWKMNRR"
     gene            941..2377
                     /locus_tag="PK016_07135"
     CDS             941..2377
                     /locus_tag="PK016_07135"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HXL02869.1"
                     /translation="MGDFFLTVDLGTTSVKLIWWSKKGEKVLEDSFPVELRFPFEGGV
                     EFDPETMVEELVKRINHSPFPLQGMGITNQRETTIVWNEEGKLVYPAIVWQDRRTATW
                     CEAHREKEDWLFQKTGLFLDPYFSLSKLVWILEKVKTSHPLYFGTLDTYVLWVLTAGK
                     VFSTDYTNASRTLMFNLHNLCWDEEIIKEFDLEKVLFPQVVPSFHLRGETQITTSSVP
                     VYVVLGDQQASFLGQGCFEKGSTKNTYGTGCFLMTNQGKDWPEDTRRLLVTLASLEGD
                     EPIFALEASTFSAGVIIEWLKRIELIKSPQDIEEMAAQARPLEELLLVPAFCGLGAPY
                     WDPFARGAILGLTPQVGKEELVRGALEAVAFEVGDLIKIIDEISPGEIKELSVDGGLS
                     QSRYLLQFQADLSGKTIRVFPEKEATSLGVFYLWANRSGLLSREFIEEKKQRALIFEP
                     RGEEKERKKLEKKWQRGIDKVRGWAKNE"
     gene            2370..3824
                     /locus_tag="PK016_07140"
     CDS             2370..3824
                     /locus_tag="PK016_07140"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXL02870.1"
                     /translation="MSEEFDVIVIGGGVVGCALSWYLAHFDLRVLLLEKELDVCSGVS
                     KANTGIIHSRSYWTPHTLKGEVHLRSLPWIKRAEKELDIEFLKTGALTVAFSQDELEF
                     LRTLKERGKIEGAEILTPQEAHTLEPMMSDKMFAAYYDPETRVASPFKLNIALAEGAF
                     LNGVRFIFGQEVVGFEEGKNNNIKVITKEGEYLSRFALNCAGLSSSYLAQRTGENIPS
                     LQPLRGEYLVLDKECKSLVHHVLYPVPVGETKGILVSPTTDGNILVGPNLEPAEDEDT
                     STTKEGLKEVEEGGKKLIPSLPLSQVIATFSGLRPTLPARDFQIFFSSRFPSLLHLVG
                     IESPGLTASFGIAEYAGDLLQKKGLKLREKRSFLFRSSFPVFRELSWEERERLIQEDA
                     DWGKIICRCEEVTVREVKHALLSSPGASTLDGLKRRVRVMMGRCQGSFCGMHLPFLMA
                     ETLGLEERKIIKSGRNSFYLGGETKEVTSDASFY"
     gene            3808..5064
                     /locus_tag="PK016_07145"
     CDS             3808..5064
                     /locus_tag="PK016_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXL02871.1"
                     /translation="MPHSIKGDVVVIGGGPAGMAAALSSWEAGAKSVFLLERNEYLGG
                     ILPQCIHPGFGLQIWGEELTGPEYAEMFIEKLNRTGVEVMTDTTALELSPERIVKAVS
                     PQGVWEIKANSIILSMGCRERTRGMLRTPGARPAGIFTAGLAQYLVNIAGYLPGREIV
                     ILGSGDIGLIMARRLTLEGARVKMVVEIKNSLSGLVRNYVQCIKDFHIPLLFSHTVTE
                     IKGKERVESVIISEVDEEGQPLPGREKEVSCDTLLLSVGLIPENELSRQAKIPLSPST
                     GGPWVDEDFSTDVEGFFACGNVASVFDLVDYVTLVAERAGRAAVLPSPSQEKYVLQAG
                     KGVHTVIPQRIREGRNGFLFIRSQKSMRKVTVSLRGEDKIFYEKRISICRPSEMVKLD
                     LSEIPWPQEKGEIKVEIRGTEIWGIS"
     gene            5027..5413
                     /locus_tag="PK016_07150"
     CDS             5027..5413
                     /locus_tag="PK016_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HXL02872.1"
                     /translation="MKLEEQKSGESLELICIVCPLGCSLSVVKDGDYVQVRGGCRRGE
                     KYAKEEITNPRRMVTTTLRIKNGVWRRVSVRTSSPFPRDKIPELMKFLSSLEVEAPQK
                     RGDIIVKNLLGEGVDLVVTRTIERKK"
     gene            complement(5414..6364)
                     /locus_tag="PK016_07155"
     CDS             complement(5414..6364)
                     /locus_tag="PK016_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02873.1"
                     /translation="MDTSTVPDLDTIRKWIDEYAKRVANLRDERGKGVELLTLRDAID
                     EGLSFYRSRGASLGPEETKLNSFDALLDKNAASFLQIAGRKTLKEERNKISPPRERWW
                     WWLDERAKERKSHRIRKIITTAGIVAAILLVLYFTIFRLPPAEQNYLDALTQAEKLIG
                     ENNIEEALENLEKAMNIFPERPVPYVIAGCVKEMVGENEEAQKFFTQARSLYSHEVDF
                     LVEKGNWYLRLNLLDKAYSSVMEALQKESENLAALNLLGSIYEAENKIPEALEVYNKV
                     LELAEKQGVETMIPITRMKIGLLQLRLPLSTFSSPPEGEQ"
     gene            complement(6377..6688)
                     /locus_tag="PK016_07160"
     CDS             complement(6377..6688)
                     /locus_tag="PK016_07160"
                     /inference="COORDINATES: protein motif:HMM:NF021402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2103 domain-containing protein"
                     /protein_id="HXL02874.1"
                     /translation="MKHRARNSKIKYEHSMIPGLRQFLEKEIEPLDFVKSIFPGEIKR
                     TKGATSGLVVRFKYLTSTGAKLLAQKSSAIQEIFIVTDEPETLKKIITASGGTNSENS
                     L"
BASE COUNT         2037 a         1359 c         1723 g         2151 t
ORIGIN      
        1 tcatttaaaa tctattccca aatgctttaa ttatagttaa ccgcaaaatc cattataatg
       61 actatgaggg ggcaagcccc ctcataaccc ccaaaaaaga ttaaaactta aaaaatataa
      121 aagtaaagac tcacaaaaac tactgaggga gaaaacatcc ctcagtggga ctgtgcccct
      181 tatagtcatt aaaaacctta tctggatcta tctttttctg cttttgattg tctttctttc
      241 taaccttcac aataaaagtt gattgtgcta caatagaaac ggcgaggaga aaaggagaaa
      301 aagccttcca gaataatatt attctggaag gcttttttgt tggcaggagg tatacatgga
      361 agatttattg aatatctggc gtgaattttc agtaattgga gctctgcgga cttcttcctg
      421 gaaagatatt aatgacgagg aactttcttg gtgcccggtc tttttctttc tggaggggaa
      481 tgtgtttact ttacgggagg cattgaaaat tttacctccc gatttacatc tcttcctgca
      541 tttagacctt tttgggggga tagcttctga cgaaagtgga ctacttttta tgcgggaaac
      601 ttttcctcag attcgaggta tcatctctac ccgggctcgt actttgaatc tggccaggaa
      661 agcaggtttc cttaccatct ttcgtttgtt tctcatcgat agtgagtctc tgcgtactgg
      721 cctccggata gcacaggaaa tttcccctga tgctctggaa atattgcccg ggattatttt
      781 ccctcaggtg aggaattcta tccctgtgga agatttacct cctcttattt gtggagggtt
      841 tatccgaaag aaagaagagg tggaagaaat aatatcccgc ggggcggttg cagtttctac
      901 cagttgccgg gagttatgga aaatgaacag gaggtaaaaa atgggagatt tttttctcac
      961 tgtagatctg ggaactacca gtgtaaagtt gatatggtgg agtaaaaagg gagagaaagt
     1021 tctagaagat tccttcccgg tagagctacg ttttcctttt gaaggaggag tggaatttga
     1081 cccggagaca atggtagaag agctggtaaa gagaatcaac cactctcctt ttcccctcca
     1141 aggtatgggg ataaccaatc agagagagac cacgatagtg tggaatgaag agggaaagct
     1201 cgtctatcct gctatagtat ggcaagaccg ccgcaccgct acctggtgtg aagctcatcg
     1261 ggagaaagag gactggcttt ttcaaaaaac cggccttttc cttgaccctt atttttctct
     1321 cagtaaattg gtgtggattt tagaaaaagt gaaaacctct caccccctgt attttggcac
     1381 tctagatacc tacgtcctgt gggttctgac cgccgggaaa gttttttcta ctgattatac
     1441 caatgcttct cgtactctta tgtttaacct tcacaattta tgctgggatg aagaaataat
     1501 aaaagagttt gatttagaaa aagttctttt tcctcaagtg gtgccttctt ttcatctccg
     1561 gggagaaacg cagattacca cctcttctgt tcctgtttat gtagttttag gtgaccagca
     1621 ggcttctttt ctgggtcaag ggtgttttga gaaaggaagc accaaaaata cctacgggac
     1681 gggttgtttt ttgatgacca accagggaaa ggactggcca gaagataccc gaaggctcct
     1741 ggttacttta gcatcactgg aaggggatga gccgattttt gccctggaag ccagcacttt
     1801 cagtgccggg gtgattatcg agtggctgaa aagaatagag ttgattaaaa gccctcaaga
     1861 tatagaagag atggcagcac aggctcgtcc tttggaagag cttcttctcg ttccagcttt
     1921 ttgtggtctg ggcgctcctt actgggatcc ttttgcccga ggcgctattt tgggattaac
     1981 tcctcaagtg gggaaagagg aattagtacg gggagcactg gaagctgtgg cttttgaggt
     2041 gggagacctc ataaaaataa tagatgagat ttctccggga gagataaagg agttatctgt
     2101 agatggaggc ctgagccaga gtaggtatct tttgcagttt caggccgatt tgagcgggaa
     2161 aactattcga gttttcccgg aaaaagaagc tacttcttta ggagtttttt atctctgggc
     2221 taataggtca ggccttctat cccgggagtt tattgaagag aaaaaacaaa gagctctgat
     2281 ttttgaacct cgaggggaag agaaggagag gaaaaaacta gagaagaagt ggcagagagg
     2341 aatagataaa gtaaggggct gggctaaaaa tgagtgaaga attcgatgtg attgtaatcg
     2401 gaggaggagt ggtaggatgt gctctctctt ggtatttagc tcattttgac ctccgggttc
     2461 ttcttttaga gaaagaatta gatgtctgtt caggagtgag taaagctaac accggaataa
     2521 ttcactctcg cagctactgg actcctcaca ctctcaaagg agaggtacat cttcgctccc
     2581 ttccctggat aaagcgagca gaaaaagaat tagatatcga gttcttaaaa accggggctt
     2641 tgaccgtagc tttttctcaa gatgagctgg aatttttacg cactctgaaa gaaagaggaa
     2701 aaatagaggg agcagaaatt ttaactcctc aagaggcaca cacactagag ccgatgatga
     2761 gcgacaagat gtttgctgct tattatgacc cggaaactcg ggtggcttct ccttttaagc
     2821 ttaatatagc tctggcagaa ggtgcttttc taaatggagt tcgttttatt ttcggtcaag
     2881 aggtagttgg ttttgaagaa gggaaaaata ataatataaa agtgatcacc aaggaaggag
     2941 aatatctatc tcgctttgct ctcaattgtg cgggattgtc ttcctcttat ttagctcaaa
     3001 gaacgggaga aaatattccc tctctccagc ctctgcgggg agaatacctg gttttggata
     3061 aagaatgcaa gagtttagtc catcatgtcc tttaccctgt tccggtagga gaaactaaag
     3121 gaattctggt ttctcccact acagatggta atattttagt agggcccaat cttgagccag
     3181 cagaggatga agatacttcc accaccaaag aaggattaaa agaagtggag gaaggaggaa
     3241 aaaagttaat cccttctctt cctctttctc aggtcattgc cactttttcc ggattgcgcc
     3301 ccactttgcc tgctcgggat ttccagattt tcttttcttc tcgttttccc tctctccttc
     3361 acttagtggg cattgaatct ccaggactta ctgcttcttt tggtatagcg gaatatgcgg
     3421 gagatttact ccagaagaaa gggttaaagc tgagagaaaa aaggagtttt ctcttccgct
     3481 cctctttccc cgtttttcga gagctttcct gggaagagcg agaaaggctc atccaagaag
     3541 acgctgattg gggaaaaata atctgtcgtt gcgaagaagt aaccgtaaga gaagttaaac
     3601 acgctctttt atcttctcca ggggcttcta ctctggatgg tttgaaaagg agagttcgag
     3661 taatgatggg aagatgccag ggaagcttct gcggtatgca tttgccattt ttaatggctg
     3721 aaacactggg gttagaagaa agaaaaatta ttaagtcggg gagaaattct ttttacttag
     3781 gcggagaaac caaagaggtg acaagcgatg cctcattcta ttaaagggga tgtggtggta
     3841 ataggaggag ggccagctgg tatggctgct gctctatctt cctgggaagc aggagcaaaa
     3901 tcggtttttc ttctggagag aaatgaatat ttaggaggga ttcttcctca atgtattcac
     3961 cccggttttg gtttacagat atggggagaa gaacttacag gacctgaata tgcagagatg
     4021 tttatagaaa aactgaatag aacaggcgta gaagttatga ctgataccac tgccttggag
     4081 ctatccccgg agcggatagt taaagcggtg agcccgcagg gggtttggga gataaaagct
     4141 aattcaatta ttttgagtat gggttgtcga gaaagaactc ggggaatgct ccgaactcct
     4201 ggtgctcgtc cggcagggat ttttactgct ggattggctc agtatctagt aaacattgct
     4261 ggctatttgc caggtaggga aatagtaatt ctaggctcag gagatattgg tctcatcatg
     4321 gctcgtcgtc tcactttaga aggagcacga gtgaaaatgg tagtagagat aaaaaactca
     4381 cttagtggtc tagttcgcaa ttacgttcag tgtatcaagg acttccatat ccccctcctt
     4441 ttttctcata cagtaactga aataaaaggt aaagaaagag tagaaagtgt aattatcagt
     4501 gaagtggatg aggaagggca accacttccg ggaagagaaa aggaagtaag ctgtgacact
     4561 cttctattat ccgtgggatt aattcccgaa aatgagcttt ctcgccaggc aaaaatcccc
     4621 ctttccccct cgaccggggg cccctgggtg gatgaggatt tttctaccga tgtggagggc
     4681 ttttttgcct gcggcaatgt ggcttctgtt tttgatctcg tggattatgt tactttagta
     4741 gcagaaagag ccggtcgggc ggcggttctt ccctcccctt ctcaggaaaa atatgtttta
     4801 caggctggaa agggagtaca cacagttatc cctcagcgaa taagagaagg aaggaatggt
     4861 tttttattca ttcgctcgca aaagagtatg agaaaggtaa cggtgtctct gcgaggggaa
     4921 gataaaatat tctacgaaaa gagaatatcg atttgccgac ccagtgaaat ggtaaaatta
     4981 gacctttcag aaataccctg gcctcaggaa aaaggagaga ttaaagttga aattagagga
     5041 acagaaatct ggggaatctc ttgagcttat ttgtattgtc tgccctttag gctgtagttt
     5101 atcagtggta aaagatggtg attatgttca agtgagagga ggatgcagaa gaggagagaa
     5161 atatgcaaaa gaagagataa ctaacccccg gcgcatggtc accactactc ttcgaatcaa
     5221 gaatggggtg tggcgaagag tgtcagtacg gacttcttcc ccttttcctc gagataaaat
     5281 tcctgagctt atgaaatttt tgagctctct ggaagtggaa gctcctcaaa aaagaggaga
     5341 tataatagtg aaaaatttgc tgggtgaagg ggtagattta gtggtaacga gaactataga
     5401 gcggaaaaaa taattattgt tccccctcgg ggggagatga aaaggtactc agaggtagac
     5461 ggagctgcag gagaccgatt ttcattcgag tgatgggaat catagtttct actccctgct
     5521 tttcggccaa ttccaagact ttattatata cctccagggc ttcggggatt ttgttttctg
     5581 cttcgtaaat agagcctaag aggttgaggg cagcaaggtt ttctgattcc ttctgtagag
     5641 cttccataac ggaagaatag gctttatcca agagattgag tctcagatac cagttacctt
     5701 tttctaccag gaaatctact tcatgagagt agagacttcg ggcttgagta aaaaactttt
     5761 gggcctcttc gttttccccc accatttctt ttacacaacc agctataaca tatggaacag
     5821 ggcgctcggg aaaaatattc atagctttct cgagattttc taaagcttct tctatattat
     5881 tttcacctat tagcttttct gcttgagtga gagcatctaa atagttttgt tctgcgggag
     5941 gaagacgaaa aatggtgaaa taaagaacta gcaagatagc agctactatc cctgcagtag
     6001 tgataatctt tctaattcgg tgagattttc tctcctttgc tctttcatct aaccaccacc
     6061 accatctttc ccggggagga gagattttat ttctttcttc tttaagggtt tttctccctg
     6121 ctatttggag aaaagaggcg gcgtttttat ctaaaagggc gtcaaaacta ttcagtttgg
     6181 tttcttctgg accgagagaa gctccccgag aacgatagaa cgaaagcccc tcatcgatag
     6241 catctcgcag ggtaaggagc tccactcctt ttcctctttc atccctgagg ttagctactc
     6301 ttttagcata ctcatcgatc cattttcgaa tggtatctaa atcgggaaca gttgaggtat
     6361 ccatatctgc agtaaattat aaagagtttt ctgaattagt tccccctgag gcagtgatta
     6421 tttttttcaa agtttcgggt tcatcggtta ctataaatat ttcttgaatg gctgaggatt
     6481 tttgagctaa aagcttcgct cccgtagaag tgagatattt gaaacgtacc accagacctg
     6541 aagttgctcc tttagtacgt tttatctctc ccggaaagat agatttgaca aaatctaaag
     6601 gttcgatttc tttttctaaa aattgtctca aacccggtat catactatgt tcatatttaa
     6661 tttttgaatt cctggcccga tgtttcatta tggctatttt tatagttttt attatagttt
     6721 aactatttat gaaaaagaat caaccccgac tataagaaga taatgcctct atcgctcttt
     6781 ttggtgattc ctgaatgtct ctatcaggaa tctaaattat tttaagggca agggcctctg
     6841 gattcccgat caaagatttc gggtatgacg aaaactaaat ttttgactct tctttccctc
     6901 tctcttcttg ggagaacctt acatatcctc tccctcgatg gagagggtga gggtgatgtt
     6961 tccaatctct ctttttaggc ttatagtgtc ccgcaaaact tgttaaactg ctaaaacttt
     7021 aaaaagcaaa ttattggaag tcaaaaaatg ggaaattacc atgcagcaag ggatactata
     7081 aattctccag tggtcttttg ctcttatgtt tttgcttttc aaaaggggtt atgaggggct
     7141 ctgccccctt ataagtcatt ataagggatt ttgcggctaa ctataaataa ttgtgctata
     7201 ctgtactcct gtagagggaa tttttggagg gtaaccgggt ttttaagata atgaaggtta
     7261 tagctgaaaa
//
LOCUS       DAOSVE010000088         7167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_5145_length_7167_cov_66.534589, whole genome shotgun sequence.
ACCESSION   DAOSVE010000088 DAOSVE010000000
VERSION     DAOSVE010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 7167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7167
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5145_length_7167_cov_66.534589"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..665)
                     /gene="clpP"
                     /locus_tag="PK016_07165"
     CDS             complement(39..665)
                     /gene="clpP"
                     /locus_tag="PK016_07165"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HXL02875.1"
                     /translation="MKDFLRMDSYLVPMVVEQTPHGERAYDIYSRLLQDRIVFLGTEI
                     DDTVANLIIAQLLFLEAANREKDINLYVNSPGGSVSSTLAIYDTIQYIKPDVATICIG
                     QAASGAAVILAAGARGKRMALPNSRVMIHQPLGGAQGQVSDIEIHTREMIKIREKLNE
                     ILAHHTGKPVEKIKADTERDYFMSPEEAREYGLIDRVLYPEEKNKKEN"
     gene            complement(662..1966)
                     /gene="tig"
                     /locus_tag="PK016_07170"
     CDS             complement(662..1966)
                     /gene="tig"
                     /locus_tag="PK016_07170"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HXL02876.1"
                     /translation="MVEVKKEEKEKHVIEMEITVEKERVNEALDKVYRELSHRMTVPG
                     FRKGKAPRGILRSFAGESFILETMTQDLIPQVVQEVLVQENLKVIGEPDLEIVQAKEN
                     EPLVFRLKVIEEPEVKLADPQELEVKKYRLEVRESDVEKEVERLIDSRSTWKDKGEEE
                     PAREGDLVKIRIEDKDYAVVAESSLDKEKFIAREVLGMKVGEKRKILGEEGKETEFEL
                     SVSAIMRKEKPEVNEDFLSSLEEGLSSIEDLKNKVREKLEALAQEMVEERLEKEAIVA
                     LAKNSEVYIPETLLDIDVRQRIDLLKEDLEKDGLTLDRYLELMNMDFAALEKHLRDLS
                     RWELRKFFVLQKYAEENRISVTEEDLMAECEEIAKRVGKTKEEVKEILESNDKIEEIK
                     DNIKSRKALRNIAQKVKVKELEEPINFVQWRTLEDPEEEMIG"
     gene            complement(2225..2956)
                     /locus_tag="PK016_07175"
     CDS             complement(2225..2956)
                     /locus_tag="PK016_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HXL02877.1"
                     /translation="MKVAILADIHGNFDALNKVAEEIVEVEDVVVLGDIIGYGAEPEE
                     CVRWVIGRKAKAVLGNHEAACIGELPLSWFNPRAAKALLWTQEHLSSFSIQFIQSLPS
                     IIPDFLQALWVHGSPRQPTEEYIDHPLIAREIFEGFSFSLCFFGHTHQAESYILREGK
                     GVERVSLLRGGALRVSPDNRYLINCGSVGQPRDGNPQASFALWDTERKVVEIKRVDYD
                     IASAAHKILHSGLPEYLAYRLERGV"
     gene            complement(2958..3572)
                     /locus_tag="PK016_07180"
     CDS             complement(2958..3572)
                     /locus_tag="PK016_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HXL02878.1"
                     /translation="MRLNVNPNRMELMKLKSRLSMAERGHKLLKDKLDALMQDFIRTV
                     RENRNLRQEIEEEIVKAFEAQSKASLLLSEEDKKLITVSSEERLGLEIQVRHVTGVRV
                     PHFEVKVEGDPYTYSLINTPAEIDEAFSSFFRVLPLMVKLAEAEKTIELLAVEIEKTR
                     RRVNALEYVLIPNLQETIKYITLKLDELARSSVVSIMRIKGSRG"
     gene            complement(3585..4973)
                     /locus_tag="PK016_07185"
     CDS             complement(3585..4973)
                     /locus_tag="PK016_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HXL02879.1"
                     /translation="MYKEFTTISEVAGSLLTVKQVEDARYMEIVEIELQDGSRRRGQV
                     LMTSKGEALVQVFEGTEGIGVGQSKVRFLGRVMELPVSPDILGRVFNGSGTPIDGGPE
                     IVPDSRLDINGNPINPVARDYPIDFIQTGISAIDGLNTLVRGQKLPIFSASGLPHARL
                     AAQIARQAKVLGEEEKFAVVFGALGITFEEANYFISELRNTGAIERSVIFVNLANDPA
                     VERIITPRMTLTAAEFLAFEMDMQVLVILTDMTNYCEALREISAARREVPGRRGYPGY
                     LYTDLATMYERAGRIRGKKGSITQIPILTMPEDDKTHPIPDLTGYITEGQIILNRQLQ
                     HKGIYPPIDVLPSLSRLRDKGIGEGKTREDHPDVANQLFAAYARGKEAEELATILGEA
                     SLTDLDRLYMKFAEEFEAKFVQQGEYENRSIEETLSRGWDLLHILPREELKRIQDVYL
                     ERFYDGESSPEE"
     gene            complement(4966..6753)
                     /locus_tag="PK016_07190"
     CDS             complement(4966..6753)
                     /locus_tag="PK016_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HXL02880.1"
                     /translation="MRLEGSTKGTVLSVNGPVVVAIGLPGAKMYDLVKVGEKKLVGEI
                     IGLDQDKVTIQVYEETAGIRPGEPVESTYEPLSVELGPGLIGNIFDGTQRPLEFIRGI
                     SGDFIARGVEVPALDRSKKWDFHPLVKVGEEVEPGDILGEVPETTTISHRVMVPLGIR
                     GKVKEIKEGSFTVEEVIAIIDQEGEEKPITLMQHWPVRTARPYKRKVNLDEPLITGQR
                     VIDTFFPIAKGGVACIPGPFGSGKTVVQHQLAKWSDADVIVFIGCGERGNEMTDVLME
                     FPELVDPYTGEPLMKRTILIANTSNMPVAAREASVYTGITMAEYFRDMGYKVALMADS
                     TSRWAEAMREISGRLEEMPGDEGFPAYLGSRIASFYERAGKVVALGKEDRTGSLTVVG
                     AVSPPGGDLSEPVTQNTLRVVQVFWGLEDRLAYQRHFPAINWLISYSLYLPHLENFWE
                     KNVAPDFSANRNLAMQILQQEAELEEIVRLVGVESLSFRERFVLEIARSIREDFLQQS
                     AFQEVDTFSSLRKQYLMLKLIITYYQEGVKALEKGLSLRDILSLPVRERIGRAKYIPE
                     EKIEALEELIEEVKEEVANKLKGEVESNV"
     gene            complement(6766..7083)
                     /locus_tag="PK016_07195"
     CDS             complement(6766..7083)
                     /locus_tag="PK016_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HXL02881.1"
                     /translation="MSDNRLAFVGGEENSLCFRGMGFDVYVAYSTEEAKEFLPVLRRS
                     KYALIFTEWKFYSLVKEQFEDQRSEALPVILGIPTRKEEMGKGSEFIRELVRVAVGSD
                     ITL"
BASE COUNT         2013 a         1831 c         1130 g         2193 t
ORIGIN      
        1 aacttcaact tttcttcttc aaacttgggc ataacctgtt aattctcctt tttatttttt
       61 tcttccgggt aaagaaccct atctatcagc ccgtattccc gggcttcttc cggagacata
      121 aaatagtctc tctctgtgtc agctttaatt ttttctaccg gtttaccggt atgatgagcc
      181 aatatctcgt tcaacttttc tctaattttg atcatctctc gagtatgaat ctcaatatca
      241 gacacctgcc cctgagctcc acctaaaggt tgatggatca tcacccgaga attaggtaaa
      301 gccatccttt taccccgagc tccagcagct aaaattaccg ctgcaccact ggcagcttgc
      361 ccaatacaaa tagtggccac gtccggtttt atatattgaa tggtatcata aatagctaaa
      421 gtagaagaaa ctgaaccacc aggactattg acataaagat ttatgtcttt ttccctgtta
      481 gctgcttcta gaaaaagtaa ctgagcaata atcaaattag ctactgtatc atctatttcc
      541 gttcctaaaa aaacaattct atcttgcaaa agacgagaat aaatgtcata agctctctct
      601 ccatgaggcg tttgctctac caccatcggc actaaataac tatccattcg caagaaatct
      661 ttcatcctat catctcctct tccgggtctt ccagagttct ccactgaaca aaattgatag
      721 gctcttccag ttctttaact ttcacctttt gagctatatt tctaagagct ttcctacttt
      781 ttatattatc ttttatctct tctattttat cattcgattc caagatttct tttacttcct
      841 ctttagtttt acccactctc ttcgctatct cttcacactc agccatcaaa tcctcttccg
      901 taaccgatat tctattctct tcagcatatt tttgtaaaac aaagaacttc cggagctccc
      961 agcggcttaa atctcgtaaa tgtttctcta aagcagcaaa atccatattc attaactcta
     1021 aatagcggtc caaagttaag ccatcttttt ccagatcctc tttcaggagg tctatgcgct
     1081 gacggacatc aatgtccaga agagtttcag gaatataaac ttcagaattt ttagccaaag
     1141 ccactattgc ttctttttcc aacctctctt ccaccatttc ttgagccaga gcttctaatt
     1201 tttctctaac tttgtttttt aaatcttcta tggaacttaa accctcttct aagctggata
     1261 gaaaatcctc gtttacttct ggcttttctt ttctcattat tgcacttact gaaagctcaa
     1321 attcagtctc ttttccttcc tccccgagga tcttcctctt ctcccctact ttcatcccta
     1381 aaacttctcg agcaataaat ttctctttat ccagggaaga ttctgctact acagcatagt
     1441 ctttatcttc tattcttatt ttaaccaaat ccccctctct tgctggctct tcttccccct
     1501 tatctttcca ggtactgcga gagtctatca atctctccac ttctttttct acatctgatt
     1561 ctctaacctc taaacgatat ttcttcactt ctaattcctg gggatcagct aactttactt
     1621 ccggttcttc tatcacttta aggcgaaaaa ctaaaggttc gttctccttg gcttgtacta
     1681 tttctaaatc tggctcaccg attactttaa gattttcctg aactaacact tcctgaacca
     1741 cttgaggaat caaatcctga gtcatggttt ctaaaataaa actttccccg gcaaagcttc
     1801 gtaaaatacc tcgaggagct ttgcctttcc taaaccctgg cacagtcatc ctgtggctca
     1861 gttctcgata aactttatcc aatgcctcgt ttaccctttc tttctccacc gtaatttcca
     1921 tctctattac atgcttttct ttttcttctt ttttaacttc taccatttat atccaccttt
     1981 cttaaaccaa agtatcaggc ttatatagta aaaaacagaa gctatatctc aaatctttct
     2041 cattgaggta taacccagtt cgttcggttt tattttacta taatctctct ggcagtcaag
     2101 aaacctacgg cctcatatag atgggtaatt ctcaaaaatc acaaaatagt tcttattttc
     2161 tttagatagg atagaaaagt aaaaaaaccg aaaaattaga accctataaa ctggacttac
     2221 aaacttatac acctctttct aacctgtagg ctaaatactc aggaagccca ctatgtaaaa
     2281 ttttgtgagc agcagaagca atatcataat ctactctttt tatttccact acttttcttt
     2341 ccgtatccca aagagcaaaa ctagcctggg ggttaccatc ccgaggttgc cctacactac
     2401 cacaatttat taaatatctg ttatcagggg atactctcaa cgctcctccc cggaggaggg
     2461 aaactctctc cactcctttt ccctctctca agatatagct ttcagcctgg tgagtgtgtc
     2521 caaaaaaaca gagagagaaa gaaaaaccct caaatatctc ccgagctatg agaggatggt
     2581 cgatatactc ctctgtgggt tggcgaggac taccatggac ccagagagcc tggagaaaat
     2641 caggaatgat agaaggtaaa gactgaataa actggatgct gaaagagctc aagtgctctt
     2701 gagtccagag aagcgctttt gcagctcgag gattaaacca agaaagaggc agctcaccaa
     2761 tacaagcagc ttcgtgatta cctagaactg ctttagcttt tctccctatc acccacctta
     2821 cacattcttc cggttctgct ccataaccga tgatatctcc taaaactaca acatcttcta
     2881 cttctactat ttcttctgct actttgttca gagcatcaaa attaccatga atgtcagcaa
     2941 gaatggcaac tttcaactca tcctctggat cctttaatcc tcataatact taccaccgaa
     3001 gaacgagcca gctcatccag cttaagggta atatatttta tagtctcctg tagattagga
     3061 atcaaaacat actccagagc gttaactcgc cggcgagttt tttctatttc caccgccaaa
     3121 agctcgatag ttttttctgc ttctgctaac ttaaccatca aaggtaaaac ccggaaaaaa
     3181 gaactaaacg cctcatctat ctcggctggt gtgtttatta agctataggt ataagggtct
     3241 ccttccactt taacctcaaa atgaggaacc cggaccccgg taacatggcg aacttgaatc
     3301 tcaagcccca gtcgctcttc ggaagaaacg gtaattaact ttttatcttc ttcagaaagt
     3361 aaaagagaag ctttgctttg ggcttcaaaa gctttgacaa tttcctcttc aatctcctgg
     3421 cgcaaattgc gattttctct caccgtgcga atgaaatctt gcatcaaggc atccaattta
     3481 tctttcagga gcttatggcc tcgctctgcc atactcaagc ggcttttgag cttcataagc
     3541 tccatacgat tgggattaac attcaatctc aagggtaacc ctcctcactc ctcaggagaa
     3601 ctctcaccat cataaaaacg ttccaggtaa acatcctgaa tccgttttag ttcttctcga
     3661 ggtaatatat gtaacaaatc ccagccccga gaaagagtct cttcgatact ccgattttca
     3721 tactctccct gctgaacaaa ttttgcctca aattcttcag caaatttcat atacaaccgg
     3781 tcaaggtcag taagagaagc ctctcccaaa atggtggcca attcttccgc ttcttttcct
     3841 cgagcataag cagcaaacaa ctggttagct acatcgggat ggtcttctcg agttttacct
     3901 tcaccaattc ctttatctct gagtcgagaa agagaaggca aaacatcaat aggaggataa
     3961 atacctttat gctggagttg tcgattcaaa attatctgcc cttcagtaat gtaaccagta
     4021 aggtcgggaa taggatgagt tttatcatct tcaggcatag tgagaatagg aatctgagta
     4081 atagaacctt ttttaccccg gattctacct gcccgttcat acatagtagc taagtcggta
     4141 taaagatatc caggataacc tcttcttcct gggacttctc ttcgggcagc agaaatttct
     4201 cgcaaagcct cgcaataatt ggtcatatct gttaatatta ccaaaacctg catatccatt
     4261 tcaaaagcta agaattcggc tgcggtaaga gtcatacgag gagtaataat gcgctctact
     4321 gccgggtcat tagctaaatt aacaaaaata accgaccgct caatagctcc ggtgttccgt
     4381 aactccgata tgaaatagtt cgcttcttca aaggtaatac ctaaagctcc gaaaactaca
     4441 gcaaattttt cttcttctcc caatacttta gcctgacggg caatttgagc agccaaccga
     4501 gcatgaggta aaccagaagc cgaaaaaata ggaagttttt gccctcttac taaagtgttt
     4561 agaccatcga tagcggagat cccagtttga ataaagtcga taggataatc tcgagctacg
     4621 ggatttatag ggttaccatt aatatccaga cgagagtcag gaacaatctc gggcccgcca
     4681 tcaataggag ttcctgaacc attaaaaact cttcctaaaa tatcaggaga aacaggaagc
     4741 tccatcactc ttcccaaaaa tctaacctta ctttgcccta ctcctattcc ttctgttcct
     4801 tcaaaaactt gcaccagcgc ctcgccttta ctggtcatca acacctggcc tctccggcgt
     4861 gaaccatctt gtagctctat ttctacaatc tccatatacc gggcgtcttc cacctgtttt
     4921 acggtaagta aagaaccagc tacttcggaa atagtagtaa attccttata cattgctttc
     4981 aacctcgccc ttcaatttat tggctacttc ctctttaacc tcttctataa gttcctccaa
     5041 agcctctatt ttttcttcag gaatgtattt tgctctccct atcctctctc gaaccggaag
     5101 agagagaatg tctcgcaaag ataatccttt ctctaaagcc ttcacgcctt cctgataata
     5161 agtgatgata agtttaagca tcaggtattg cttgcgcaaa gagctaaaag tatctacctc
     5221 ctggaaagca ctctgttgta gaaaatcttc tctaatagac cgagctattt ccaaaacgaa
     5281 cctctctcgg aaagagagcg attctactcc taccaaacgg actatttctt ctaattctgc
     5341 ttcctgctgg agaatttgca tagccaaatt acgattggca gaaaaatcag gagctacatt
     5401 tttctcccag aaattctcca aatgaggaag ataaagggaa tagctaatca accagttaat
     5461 agctggaaaa tggcgctgat aagccaatct atcttccagc ccccaaaata cttgcactac
     5521 gcgcaaagta ttttgagtta ctggctcaga caagtcacca ccggggggtg atacggcacc
     5581 aactacggtc aaggaaccag ttctatcttc ttttcccaaa gcaaccactt tcccggctcg
     5641 ttcataaaag ctagctattc ggcttcctaa ataagccggg aatccttcgt ctccaggcat
     5701 ttcttccagg cgcccagaaa tctctcgcat agcttccgcc cagcgagaag tagaatcagc
     5761 cataagagct actttgtaac ccatatcccg gaaatattca gccatagtaa tcccggtata
     5821 aactgaagcc tcacgtgctg ctaccggcat attagaggta ttggcaatca aaatagtcct
     5881 cttcataaga ggttccccag tataggggtc aactaactcg gggaactcca tcaacacatc
     5941 ggtcatctcg ttaccccgtt ctccacaacc aatgaagacg atgacatcag catctgacca
     6001 tttagctaat tggtgctgca ccactgtctt tccacttcca aaaggaccag gaatacaagc
     6061 tactcctcct ttggctatgg ggaagaaagt atcaataact cgctgcccag tgattaaggg
     6121 ctcatctaaa ttaacttttc ttttgtaagg acgagcagtt ctaaccggcc agtgctgcat
     6181 caaagttatg ggcttttctt ctccttcctg gtcaattatg gcaatcactt cttctacagt
     6241 gaaagaacct tcttttatct ctttaacttt tcccctaata ccaaggggaa ccatcactcg
     6301 gtgagaaata gtagtagttt cagggacttc tcctaatatg tctcctggct ctacttcttc
     6361 tcctactttg actaaggggt gaaaatccca ctttttggag cgatccaatg ccggaacttc
     6421 tactcctcga gcaatgaaat ctccagaaat ccctcttata aattccagag gtctttgtgt
     6481 tccatcaaaa atgttaccta tcaaccccgg gcctaactcc acactcagcg gttcataagt
     6541 tgactctact ggctccccgg gtcggattcc tgctgtttcc tcataaacct gaatggtaac
     6601 tttatcctgg tcaagaccaa taatttctcc caccagcttt ttttctccca ctttaaccag
     6661 gtcatacatc tttgccccgg gaagtcctat agccactacc actggcccgt tgacgctcaa
     6721 taccgtacct ttagtgcttc cctccaacct catcattcct ctcttctata aagttatatc
     6781 gcttccaact gctacccgca ccaattctct tataaattca gaaccctttc ccatctcttc
     6841 cttccgagtg ggtattccta aaataaccgg caaagcttca gagcgctggt cttcaaactg
     6901 ttctttaacc aaagaataaa acttccactc ggtaaaaatc agggcatact tacttctccg
     6961 caagacgggt aaaaattcct ttgcctcttc agtagagtaa gctacataaa catcgaaccc
     7021 catacctcga aaacagaggc tattttcttc tcctcctaca aaagctaaac gattatcact
     7081 catagtaaca ctctcgtaat ttatttttta ttttttcatt ttctaaacca aaatactttc
     7141 cttccagaat caaccgcaaa ttcttaa
//
LOCUS       DAOSVE010000089         6892 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_5351_length_6892_cov_70.919994, whole genome shotgun sequence.
ACCESSION   DAOSVE010000089 DAOSVE010000000
VERSION     DAOSVE010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 6892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6892
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5351_length_6892_cov_70.919994"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            713..1225
                     /locus_tag="PK016_07200"
     CDS             713..1225
                     /locus_tag="PK016_07200"
                     /inference="COORDINATES: protein motif:HMM:NF013234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HXL02882.1"
                     /translation="MVHGKLIICFLAVSVREPKNFEEVSIMIKVIGSQHATSPPKIHR
                     PLEVKKPWGKFIRYTLNTPTTVKILEINPGEILSLQSHNYRDELWIPLNERVVAEIDG
                     EIYFPKELEPIFVPKHSKHRLSAKDKKVRVLEISFGYFDEEDIVRYEDKYGRTKDELI
                     EILEQRASGQ"
     gene            1225..2418
                     /locus_tag="PK016_07205"
     CDS             1225..2418
                     /locus_tag="PK016_07205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXL02883.1"
                     /translation="MSRSLPIGFLSTYPPTPCGIATFTGDVVRAISSFPFAPQIMAVS
                     QSEQAVTYPEEVKVVIQKERIEDYQKAAEYANKELKMVNIQHEYGIFGGADGEMVIEF
                     LDKLRIPAVTTLHTVLCQPSSHQAEVLQKIIQRSEKVVVMNSLAIPILKDKYRAPSEK
                     MVVINHGAPSACLYQKEEQKVALGFGDRIVLSTFGLISRGKGIEYAIQALPEVVHKFP
                     QVIYLIIGITHPQVKAKEGESYRNFLEQLVKELSLEKHVIFVNRYLGKEELIKYLVAT
                     DIYLTPYLNSQQIVSGTLAYAMRFGKVIVSTPYLYAQELIGKNRGILVNFADSQSIKD
                     ALVGLLENPLYFRQIEKNAQEFGAKLEWSEVGRDYAQLFDEVINKKTASSLGEINLIS
                     QVNHR"
     gene            2462..3535
                     /locus_tag="PK016_07210"
     CDS             2462..3535
                     /locus_tag="PK016_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02884.1"
                     /translation="MNILQEKKVFSSIKLDYLASLSDHVGLLQHADYKLPKFEFGYAT
                     DDNCRGLLVVAKFYSIYPDPLAKKLAQRYLAFLRWVQKEDGQFHNEVSIDRRFLDVVG
                     SEDCQCRTFWALSATLNTTIAEDFKSVIKEILEKFLPNLFSYSALRPLAFLILGLREL
                     SRQKENIFSGLDILNTTRTLAERLAEQYERNASPQWAWFEEAMTWGNALLPASLFVSY
                     RLLGDKKLLEIAEESFDFMSNHVFDQQLIFQPVGNRGWFPREGKKAQFDQQPIEAMWM
                     TIAAEEAFEVTGKSHYRRKARSAIEWFGGRNCHRIPLYDSEDGSCADGLTPDGINSNR
                     GVESTLSALCSFLWAKEKGILGN"
     gene            complement(3748..6018)
                     /locus_tag="PK016_07215"
                     /pseudo
     CDS             complement(3748..6018)
                     /locus_tag="PK016_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018759257.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
BASE COUNT         2060 a         1367 c         1340 g         2125 t
ORIGIN      
        1 agtatccaga aagtctgttg tttacaataa gggaggaaaa taccccttat gactctcgca
       61 agaagtcaaa aaaaatataa aagacaaaaa ccaccggggg tatagactta ctgtaacccc
      121 tcttatctgt cactccggcg acttgcgagc cgtttttagc ctgcccctga atgactctat
      181 cggctatagg agggaaaaga ccccctatga cccccggaaa gacaaaagaa agagagcaaa
      241 tacaacccgg agaaaagacc tccgtgttcg tcacccctga atggttctgc gaggggtcca
      301 cagtatttaa aaaccaaaga aaaagtaaaa acttctggat gcctgataaa agcgctcagg
      361 catgacgaaa gaggcgtcat tcctgaatgt atctatcagg aatccaaact atagggggga
      421 agctgatccc cctatcaccc ccttggaaaa acaaaaactt ggattcccga taaaaaattt
      481 cgggaatgac ggcggggggt ggattcccga ttactgattt cgggaatgag ggagatagga
      541 agtcctcgac aaaagtgaga ttgccacgct cagaaggaag ttcttgctcc ttttggggtt
      601 tttacgtctt tattctttag tcttacgccg tagactgcca ggcgaggctc acaattggct
      661 ctggggaaag gagagagggg taaatttgac attgactttc tttttgacta taatggttca
      721 tggaaagtta ataatttgtt tccttgctgt ttcagtgagg gaaccaaaaa actttgagga
      781 ggtgagcatt atgatcaaag ttattggttc ccagcatgca acttctcctc ctaagattca
      841 tcggccttta gaagtgaaaa aaccttgggg taagtttatt cgatatacct taaatactcc
      901 taccacggta aaaatactgg aaataaatcc aggagaaatt ttaagccttc aatctcacaa
      961 ctatagagat gaattgtgga taccccttaa tgaaagagta gtagcagaga tagatggaga
     1021 aatttatttt cccaaagaat tagagcctat ttttgttcct aaacattcta aacatcgtct
     1081 cagtgctaaa gataagaaag ttagggtttt ggagatttct tttggttatt ttgatgaaga
     1141 ggacatagtt cgctacgaag ataagtacgg gcgaacgaaa gatgaattga ttgaaatttt
     1201 ggaacagagg gcgagtggac agtagtgtct cgctctctac cgatagggtt tttatctact
     1261 tatcctccca ctccttgtgg catcgctact tttactggag atgtggtccg ggcaatatct
     1321 tcttttcctt ttgctcctca aattatggct gtatctcaaa gtgagcaggc cgttacttat
     1381 cctgaagaag ttaaggtggt catccaaaaa gaaagaatag aagattatca aaaagcagca
     1441 gaatacgcta ataaagaact gaaaatggta aacatccagc acgaatatgg catttttgga
     1501 ggagcagatg gggagatggt gatagaattt ttagataaat taaggatacc agcagttact
     1561 actttacata ctgttctttg tcaaccttct tctcatcagg cggaagttct ccaaaaaata
     1621 attcagcgat cagaaaaagt agtggtaatg aatagcttgg ctattcctat tttaaaggat
     1681 aaatatcgag cgccttcgga aaaaatggta gttataaacc atggtgctcc gtccgcttgc
     1741 ctttaccaga aggaagaaca aaaagtggcc ttgggttttg gcgatagaat tgttttgtcc
     1801 acttttggat tgattagtcg gggaaagggg attgaatatg ctatccaggc cttacctgag
     1861 gtggtacaca aattccctca ggtaatatat ctgataatag gaattactca tcctcaagtt
     1921 aaggcaaaag aaggagaatc atataggaac tttttagaac aattagtaaa ggaattaagt
     1981 ttagaaaaac atgttatttt tgtgaaccgt tacttgggta aagaagaatt aatcaaatat
     2041 ctggtggcta ctgatattta ccttactcct tatttgaact ctcaacaaat tgttagtggt
     2101 actttagctt atgctatgcg gttcggaaaa gttattgttt ctactcctta cttatatgcg
     2161 caagaattga taggaaaaaa taggggaata ctggtaaatt ttgctgattc tcaaagtatt
     2221 aaagatgctc tcgttgggct tttggaaaat cctctctatt ttcgtcaaat agaaaaaaat
     2281 gctcaagagt ttggcgctaa gctggaatgg agtgaagtgg gaagagatta tgctcaatta
     2341 tttgatgaag taattaataa aaaaaccgct tcctccttgg gagaaatcaa cttaatttct
     2401 caagtaaatc atcgttagct gaaagttaac taagatagga atgaaaagag gtaaaatagg
     2461 catgaatatt ttacaggaaa agaaagtttt ttcctcgatc aagttagatt atttagcttc
     2521 tctcagtgac cacgttggcc ttttacaaca tgctgattat aaacttccca aattcgagtt
     2581 tggatatgct actgatgata attgtcgagg actcttggtg gtggctaaat tctactctat
     2641 ctaccctgat ccgttagcca agaagttagc tcaacggtac ttagctttcc ttcgctgggt
     2701 tcaaaaagaa gatggccaat ttcataacga ggtttctatt gatcgacgtt tcctggatgt
     2761 ggttggttcg gaagattgtc aatgccgaac cttctgggct ttatcagcca ctttaaatac
     2821 tactattgct gaagacttca aatcagttat aaaggagata ctggaaaaat ttcttcctaa
     2881 cctcttttct tattctgcgc ttcgtccttt agctttttta attctgggat tgcgggaact
     2941 ctctcggcaa aaagagaaca tcttttctgg cttagatata cttaatacta ctcgcacctt
     3001 agcagaaaga ttggctgaac aatacgaaag aaacgcttct cctcagtggg cctggtttga
     3061 agaggcaatg acctggggta atgctctttt gcctgcctct ctctttgttt cttatagact
     3121 tttgggagat aaaaaattat tagagatagc tgaagaaagt tttgatttta tgagcaatca
     3181 cgtatttgat cagcaactta tatttcagcc cgtgggaaac agaggatggt tcccccggga
     3241 agggaaaaaa gcccagtttg accaacaacc tattgaagct atgtggatga ctattgctgc
     3301 agaagaggca tttgaggtaa ctggcaaatc tcactaccgg cgcaaagctc ggtctgctat
     3361 tgagtggttt ggaggaagaa actgccatcg tatccctctt tatgactctg aagatggctc
     3421 ttgcgctgat ggcttaactc cggacgggat taattctaac cggggggtag aaagtacact
     3481 ttctgcttta tgcagctttc tttgggctaa agaaaaaggg attttaggca actaatggtt
     3541 cttgtataag aaacttttta cttaaaacca ccttaatagg aatggggttg acttttgaat
     3601 taaaccagcc aagaattata ttaatgacgc tcaggtctga ggatttcggc tgaagttgac
     3661 tcccaaaaca ggatattgct ttacctcccg ggttagtttt tcaggaaaga aagcaatatc
     3721 ctgtttttta tttaactctt ctattaacta tttgagaggg aaatctttat taaaaacata
     3781 ttttttaact cgttcatcga taattcttcc gctccaattg aggctaaaac caaaattgta
     3841 ggtatttcct tcggagtcta cgtaacattc catatcatgg gggacatctt cgtattgtaa
     3901 ttccactgtt ttcatatttg ctggcatttg tataggtaat agcccttgcg gttcaaactt
     3961 tcctgttaga gcatcaaaca ctgcttgggt ttgaacatca aaagtaatca gtagtgcatc
     4021 tatgtaattt tcaaattctc caacaattgt tggttttgac atgttgagca ctacaaccac
     4081 aggtttacca tccatagctc tatatgtatc taagaccata aacaaatcgt aaatgttaga
     4141 agcaacaaca cttttacctt tatatgttct gtttttaaaa tcttctaatg ggtctccgcc
     4201 agctaaacta attgctctcg cataaaccgc cgtgtaggga ctatactgaa gacttatagg
     4261 aacgtatcca ttccctccag aggcaaggtc atcagggtca taaccggcac cggtttttgg
     4321 agtttctata aagcagagtg caaaatctgc ttcttcgggg ttttctgtta cagcaaaata
     4381 tttttttgca atttcgatag aaatagggta gtcataagat tcttctataa tattaccaaa
     4441 gaagtccttt gacgctggag aatatctttt tggtatgtaa acttttttcc cggcttctaa
     4501 aggaagtata ttatctttgt tttttaacat tactattgat ttaagttgtg cttcataacc
     4561 tgctgccatg tattgtggat tacccacggt ttcctttgtt tcttctatat cgagataggg
     4621 attttcaaac aggcctactt ggaatatatt ttttagaagc ctttttgcaa attgttcaaa
     4681 tcgttgccgc ataaattctt ctccatattc ttttataccc aatttatagg cctctattat
     4741 tggttcagct ttattttgtt tcctccgaac tggtccacgc cagccattat ggctttataa
     4801 tgtctttcta cttcggttaa gttttcgaca ccccagggtc tggtctgctc tggaaagaat
     4861 caacggtagg tccctggtca gctgtaatcc cccagtcggt acaaatcact ccatcatatt
     4921 ggtattttgt tcgaagcaag tcattaatca tatatttact ataagaattt cctacatttt
     4981 ctttgtaaat aatgtcctga ttatatgaga ttgtatagta tggcataatt gcagcggttt
     5041 ttcagtttct tcttccaaat caagagcacc attcacaaag ggtattaact gcatatcaaa
     5101 attgtttcct ggatatactg catattttcc ataagagtaa tgtgcatctc ttcctccttc
     5161 tcctgttcct ccaccaggcc aatgttttgc cattgtgttt acactttcat acccccagcc
     5221 attcattatt tctttctcac cataagaagt ttgaaagctg tctacataag ctcttgccat
     5281 atcagctgca agctcagggt cttccccaaa agttccattt actcgacccc atctcgggtc
     5341 tgtcgcaagg tcaatttgag gagacagagc agtggcaata cttaatgctc tatgctctct
     5401 tgaagcgatt tttccaaaat ttttaactaa ctcaggatca aatgtagccg ctaaccccag
     5461 tgaattaggc cacatagaga tactctctcc cgcttctgca ttatattcag tatctgcaat
     5521 tgctccgtga cgggggtccg aacttatatt aaccggtatt ccataaccaa aactttctgc
     5581 cagagcctgg agattattat tccatcttgc tgcagcttcg ggactctcga cagatgtgat
     5641 taaaacatgt ctgagattat cttttgttaa aaattccttt tgctggttag ttagatccca
     5701 ggcagaagcg ccactttcct ggtaagactt tccattatag gttcctccga accacccagt
     5761 ttccggcaca gcttgatggc tgctatagag cataagcccg gcaatttgtt ctatggttaa
     5821 ttttgaagct aaatcttttg ctctttcttc gggggataac ctccaatctt catagtcatc
     5881 aagcacacca tttttattta aatctttgaa gtattttcca tcaacttcaa taatctttac
     5941 ccctgagcta tctgaatagc caagaacctg accatcctca ttttttacat aagaaatacc
     6001 atctgaagcg tataatatat aaaagggaaa tattacagtt catgctgcaa gaaaacctat
     6061 tttgacacct cttttaatct tcaatttttc tccctcctaa cttattttta atcaaaaaac
     6121 ttcatgtgtt taaagtatac catttttgta attatagtta accgcaaaat ccattacaaa
     6181 tactataaag gggctgtgcc cctttatcat ccccctttct ttttcttccc tcgccttcaa
     6241 tggaagaacc ccacatattc cttccctcgc ccttctagga agagggttag ggtgagggtg
     6301 ttttcttgcc gttattcctg aatggttcta tcgactatga ggaggcaagc ccccttataa
     6361 cccccaaaaa agattaaaac ttaaaaaata taaaagtaaa gactcaaagg ctactgaggg
     6421 aaaaaacatc cctcagtggg cagtgcccct ttataatccc ccaaaaagca gacagcctac
     6481 ggcgtaggat taaagactta aaaactaaaa gcaaaaagag taagcataaa aaactactgg
     6541 aagagaaaaa gtgtctcttc cagtacaaag gggaaagaat tcccccttgt aaaccccttt
     6601 ctttttgcca gtgcaataac cttcttgcca ctcctgaatg cctctatcga ctatgagggg
     6661 gtttcacccc cttataatcc ccaaaagcaa aaaattaaaa actgaaaaga caaagactac
     6721 tgaggaagaa aacgctttcc tcagtacctg cggcaccaaa gacgaaagca aaatagatga
     6781 aagaaactaa aaccaattac gctggagaaa agcactccag cgttgaggaa ggaaaagcgt
     6841 ctttctcagt ataaggggga aagaattccc ccttatgaat cccctttctt tt
//
LOCUS       DAOSVE010000090         6776 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_5430_length_6776_cov_80.043892, whole genome shotgun sequence.
ACCESSION   DAOSVE010000090 DAOSVE010000000
VERSION     DAOSVE010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 6776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6776
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5430_length_6776_cov_80.043892"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PK016_07220"
     CDS             <1..647
                     /locus_tag="PK016_07220"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXL02885.1"
                     /translation="EEAKKLAKEKGISSIDFEIGKVQDLSRFPDRSFDLVLSLDAPVS
                     YAYPEEKKALRELTRVASDKLIISVVNRWGQIPVAVETELRFRNNLEISRRFFQEGNW
                     DHPSFWENLERKVPFLCRFVFPPLHAFTPSEIIDDLIEEGFRPLRVVASGTLARLLSR
                     RTLKKIVNNPSLYREFLEFSREYDALFEVLGVGAKVASGLLVVGERKNSDEKMV"
     gene            631..1437
                     /locus_tag="PK016_07225"
     CDS             631..1437
                     /locus_tag="PK016_07225"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HXL02886.1"
                     /translation="MRKWYKTTITIGEEKEEELLLQLQGQNITGIWELRPGEIIVYSE
                     SSLTLPPEIEKIEEEEEEGEWAYKWREGFKPVRVGERIVVRPPWEDPQDVAVELVIYP
                     AYAFGTGQHPTTYWCLYMIDKHFQKGQSFLDVGSGSGILSILAAKLGGGRIVALDIDP
                     HAGEELKRNLSLNGIDFSRIDFQEKEVEEVKEKFDFIVCNIGTNFHLFHLETMKNLLN
                     EGGILVLSGGEEKDFFLLQKKAKMLSLKEKEVRIDASWVTVVYKKSENVL"
     gene            1461..1868
                     /locus_tag="PK016_07230"
     CDS             1461..1868
                     /locus_tag="PK016_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02887.1"
                     /translation="MRVAFVHSGKDKRVAELARYMVGLFEKEGCTVKIIEAESTASPV
                     SLRPFDLIFVGSQVFSLWGGKISQEVVNFLQGASGMEGKRAVAYVRPNLFGTDKALRR
                     LMSKMESQGAFVVDFEVLKGEKEAENLVKRHLK"
     gene            1885..2088
                     /locus_tag="PK016_07235"
     CDS             1885..2088
                     /locus_tag="PK016_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02888.1"
                     /translation="MEEKEKAREAFEEIFFKPMEKVLSPFLNEEARKHFSQARVEVLK
                     AMRAFIDAEIERADKKEKKSSES"
     gene            2202..2405
                     /gene="secE"
                     /locus_tag="PK016_07240"
     CDS             2202..2405
                     /gene="secE"
                     /locus_tag="PK016_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HXL02889.1"
                     /translation="MKRLGKWFRSLKQYFRDAWSELRRVTWPSRKELWNSTLTVLVVI
                     ALAGVFLGAVDLLLTFLVNLYLR"
     gene            2428..2952
                     /gene="nusG"
                     /locus_tag="PK016_07245"
     CDS             2428..2952
                     /gene="nusG"
                     /locus_tag="PK016_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HXL02890.1"
                     /translation="MSKQWYVVHTLAGSEHKVKANLERRITSMGMQDQIFRVVVPMEE
                     AIEVKRGKKRFTKKKVFPGYVMVEMIMNDRSWYVVRNTPGVTGFVGSGMKPEPLNEEE
                     VNVILRQTGVEKGVLRLDVEKGEMVKVIAGPFLNYTGTVESVDQEKGKMTVLLSIFGR
                     ETPVELDFSDVEKL"
     gene            2971..3399
                     /gene="rplK"
                     /locus_tag="PK016_07250"
     CDS             2971..3399
                     /gene="rplK"
                     /locus_tag="PK016_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HXL02891.1"
                     /translation="MAKKIIAVVKLQIPGGMATPAPPVGPALGQHGVNIMEFCKAFNA
                     ATEKDRGILTPVEITIYGDRSFTFVCKTPPASFLIKQALGVEKGSGEPNRVKVGKIPR
                     SKIREIAEKKMPDLNANDLEGAIKIIEGTARSMGVEVTEG"
     gene            3416..4132
                     /gene="rplA"
                     /locus_tag="PK016_07255"
     CDS             3416..4132
                     /gene="rplA"
                     /locus_tag="PK016_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HXL02892.1"
                     /translation="MADRGKRYLTAKNKVEAGKLYDPETAIRLVKEMANARFDETIEV
                     AINLGIDPKQSDQQVRGTVSLPHGVGKIVRVLVFAQGEKAREAQEAGADYVGGEELAD
                     KIQGGWLDFDAAVATPDMMRVVGKLGKVLGPRGLMPNAKTGTVTFDIGQAVKEIKAGR
                     IEIRNDRYGNIHAPIGKASFTVEDLLDNFYALIDAIVRLKPSTSRGRYIKKIVVSSTM
                     GPGIPVDPNLALGRLEKRVA"
     misc_feature    4146..4314
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            4329..4847
                     /gene="rplJ"
                     /locus_tag="PK016_07260"
     CDS             4329..4847
                     /gene="rplJ"
                     /locus_tag="PK016_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HXL02893.1"
                     /translation="MQKAEKEKIWQEVCEKLQKSQAVFVINYQGIKVENMNELRRSLR
                     NAQGEIKVVKNTLARIALDKAGISYDDELLKGQNALAFSYLDAVQIAKLLADAGKKLP
                     QLEIKGGWLDRNPLKPEEVKRLAELPSREQLIAQVVGGMKAPIAGLVGVLQGLLQNLV
                     GVLHAIEEKKNA"
     gene            4862..5239
                     /gene="rplL"
                     /locus_tag="PK016_07265"
     CDS             4862..5239
                     /gene="rplL"
                     /locus_tag="PK016_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HXL02894.1"
                     /translation="MTKEEIIESIEKMTVLELAELVKALEEKFGVSAAMPVAQVAAGG
                     AQGPATPEVEEKTEFDVVLKSVGEQKLQVIKEVRAITGLGLKEAKELVDNAPRSVKEA
                     VSKKEAEEIKAKLEAVGAEIEIK"
     gene            5278..6186
                     /locus_tag="PK016_07270"
     CDS             5278..6186
                     /locus_tag="PK016_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXL02895.1"
                     /translation="MNKKYLLNPYFLLILVSLIWGFNFSVVKIGILTIGPLAFSLLRF
                     ILSALLMFLVLWLTEKNPWVEVKDLPYFLFLGLLGFGIYQPLWSYGLKLSLASHSALI
                     LSLSPIVVSLVAFVRKEELVGGINLLGVIIGFGGVIFLVGQGGNGDYAPQSVLYGDLL
                     TLGAAICWGLYSYFGKNMVKKYSPLKTSTWSVIWGLVFMFPVCIGETLQVTREDFSFS
                     LLLSLSYSVVLSSLVAYIIWMQCVKELGAARTISSQYITQVVGVIAAWMIFGEPLGWR
                     VFLGMAFISLGLWLIHKKPSGGELIL"
     gene            complement(6400..6690)
                     /locus_tag="PK016_07275"
     CDS             complement(6400..6690)
                     /locus_tag="PK016_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02896.1"
                     /translation="MAPSLTAEPGAKVTDSFMEDLLTYKTELETGQLVSNHADAYQTF
                     FTIKETPVRGRKVLFNQEAIQKYRNRYAGFYVLLTNDIKDPVEALRVYRNKG"
BASE COUNT         2025 a         1077 c         1751 g         1923 t
ORIGIN      
        1 tagaagaagc aaaaaagtta gcaaaagaaa agggtatttc ttctattgat ttcgagatag
       61 ggaaggtgca ggatttatct cgtttcccgg accgctcttt cgacctggta ctttccttag
      121 atgctccggt ttcttatgct tatccggagg agaaaaaagc tctccgggag ttaactagag
      181 tagccagtga taaattaatt atatcggtgg taaatcgctg ggggcaaatt ccagtagcag
      241 tggaaacaga attacgattc agaaataatt tagagatttc tcgacgcttt ttccaggaag
      301 gtaactggga ccatccctct ttttgggaaa atctggaaag gaaagttcct tttctttgcc
      361 gcttcgtttt tcctcctctg catgctttta ctccttctga aatcatagat gatttgatag
      421 aagaaggatt taggccttta cgggtggtgg cctcgggaac cctggctcgt cttttatctc
      481 gtcgtacttt aaaaaaaata gtgaataatc cttccctgta tagggagttt ttagagttct
      541 cccgggagta tgatgctcta ttcgaagtgt tgggagtggg ggcaaaagta gcttcaggtc
      601 ttttggtggt gggggaaaga aaaaatagcg atgagaaaat ggtataaaac cacaataacc
      661 ataggggaag aaaaagaaga agagctttta cttcaattac aaggacaaaa tataaccgga
      721 atatgggaat tacgacctgg tgaaataata gtttactcag aaagttctct tactctccct
      781 ccagagatag agaagataga ggaggaggaa gaagagggag agtgggctta caaatggcga
      841 gaaggtttta aacctgtccg ggtgggagaa agaatagtgg tgagaccccc ctgggaagac
      901 ccacaggatg tagcagtgga gctcgtaatt taccctgctt atgcttttgg taccggtcaa
      961 catcccacca cttactggtg tttatatatg atagataaac acttccaaaa agggcaaagt
     1021 tttttggatg tgggaagtgg ctcggggatt ctttctattt tagcagcgaa gcttgggggg
     1081 ggaagaattg tagctttaga tattgatccc catgctggag aggagctgaa gcgcaattta
     1141 tctttaaatg gaatagattt ttctcggata gattttcagg aaaaagaggt agaagaagtt
     1201 aaagaaaaat ttgactttat agtttgtaat atcggaacta attttcatct ttttcacctg
     1261 gaaaccatga aaaatttact taatgaaggg ggtattctgg ttctctctgg aggagaagag
     1321 aaggattttt ttcttcttca aaaaaaagct aaaatgttga gcttaaaaga aaaagaagtc
     1381 aggatagatg cctcctgggt taccgtagtt tataagaagt cggaaaatgt attataatat
     1441 aaaaggaggt gaatgcattt ttgcgagtag cttttgttca cagtggcaaa gataagcgtg
     1501 tagccgaact ggcccgctat atggttggcc tttttgaaaa agaaggttgc acggtaaaaa
     1561 ttattgaagc ggaaagtacc gcttctccag ttagtttgcg tccttttgat ctgatatttg
     1621 ttggttctca agtttttagt ttgtggggag gcaaaatatc tcaagaggta gtgaactttt
     1681 tacagggagc aagcggaatg gaaggtaaaa gagcagtagc ttatgttcgt cccaacctgt
     1741 tcgggacgga taaggcttta agaaggttga tgtctaagat ggaatcgcaa ggagcttttg
     1801 tggtggattt tgaagtacta aaaggtgaga aagaagcgga aaacctggtt aaaagacacc
     1861 tgaagtgata gggaggtgaa ggctgtggag gaaaaggaaa aagctcgaga agcatttgaa
     1921 gagatatttt ttaaacccat ggaaaaagtt ttaagccctt ttctcaatga ggaagctcgc
     1981 aagcattttt ctcaagcaag agtagaagtg ctgaaagcta tgcgtgcttt tatagatgca
     2041 gaaatagagc gggcagataa aaaagagaag aaaagctcag aatcttaagg ataaaacatt
     2101 gatagcttaa ttgacagata ggaagaaaaa acttataatg cctcagtggc tttggactgg
     2161 agaaaagttg ttttttctaa aagtgtaaaa tagaaggatg ggtgaaacgg ttgggcaaat
     2221 ggtttcgttc gctgaaacag tattttcgtg atgcctggag tgaattacgt agagtaacct
     2281 ggcctagcag gaaagagctc tggaacagta ctttgactgt attggtagta attgcattag
     2341 cgggggtgtt tttgggagcg gtggacttat tgcttacttt tttagtgaat ctttatctaa
     2401 ggtgatgtgg tagtgaatga agcagaaatg tcaaaacagt ggtatgtggt tcatacttta
     2461 gcaggtagtg aacataaggt aaaagctaat ctggaacgga gaattacttc tatggggatg
     2521 caggatcaga tttttcgggt agtagttccc atggaagaag caatagaggt gaagagggga
     2581 aaaaagagat ttaccaagaa aaaggtcttc ccgggatatg ttatggtgga gatgataatg
     2641 aacgatcgtt cttggtatgt ggtgcgtaat actccaggag ttactggttt tgtaggttcg
     2701 ggtatgaaac ctgaaccttt gaatgaagaa gaagttaatg tcattttgcg tcagaccggg
     2761 gtagaaaaag gagttcttcg tctggatgta gaaaaaggag agatggtaaa agtgattgct
     2821 ggtccttttt tgaactatac cgggactgtg gaaagtgtag accaggaaaa aggcaaaatg
     2881 accgtgcttc tctccatttt tggccgtgaa acaccagtgg aactggattt ttctgatgtt
     2941 gagaaactgt aaagaggtgg cagagaaaga atggcaaaaa aaataattgc ggtggtaaaa
     3001 ttgcaaatcc cgggggggat ggctactcct gctcctccag tgggtccagc gttaggtcaa
     3061 catggggtta atataatgga attctgtaaa gctttcaatg ctgctacaga gaaggaccgg
     3121 gggatattaa ctccggtgga aattaccata tatggggatc gctctttcac ctttgtatgt
     3181 aaaacccctc ctgcttcttt tttaatcaaa caggctctgg gggtggaaaa aggttcagga
     3241 gagcctaacc gggtgaaagt aggtaaaatc cctcgttcta aaattcggga aattgctgag
     3301 aaaaaaatgc ctgaccttaa tgctaatgac ttagaaggag caattaagat tatagaagga
     3361 acagccagaa gtatgggagt ggaagtcaca gaaggataac gaggaggaat ggaaaatggc
     3421 agatagaggt aagagatatc tgacagcaaa aaataaagta gaagcaggca agttgtatga
     3481 cccggagacg gctattcgcc tggtgaaaga gatggctaat gcccgctttg atgaaacaat
     3541 agaagtggct ataaatttgg gaattgaccc caaacaatct gaccagcagg ttagggggac
     3601 ggtttccctt cctcatggag tgggcaaaat tgtgcgggtt ttagtttttg ctcaagggga
     3661 aaaggcccgg gaagcacagg aggcaggtgc tgattatgtg gggggagaag aactggcaga
     3721 taaaatccag ggtggatggt tagattttga tgctgcagtg gccactcccg acatgatgag
     3781 ggtagtgggg aagttaggta aagttttagg accccggggg ttgatgccta atgctaaaac
     3841 tggtacagta acttttgaca taggacaagc ggtaaaagaa ataaaagcag gacgaataga
     3901 gattcgcaac gaccgttatg gtaatattca tgcccctata ggaaaggcct cttttacggt
     3961 ggaagattta ctggacaatt tctatgccct tatagatgcg atagtacgct taaaaccatc
     4021 tacttctcga gggcgttaca ttaaaaagat agtcgtttct tctaccatgg gaccaggtat
     4081 accagtagat cccaatttag ctttagggcg tctggaaaag agggtagcat aaaaattgag
     4141 taaaattgaa atttgaatac cggcctgaga accgggggca ttagcttaaa tatcccgggg
     4201 aggtaagagt agaaaacttt tctcatggtg tttggtgaag ttttctgaga aaagtgagta
     4261 ttaaacatta actgaaaagg ctcttctccc ggaagagcct ttttttatgg gaaggaggta
     4321 gatagttctt gcaaaaggca gagaaagaaa aaatctggca ggaagtttgt gagaaactgc
     4381 aaaagagcca ggcagtgttt gtgattaatt atcagggaat taaagtggaa aacatgaatg
     4441 agctccgccg gagcttgaga aatgctcagg gagaaataaa ggtagttaaa aacaccttgg
     4501 ctcgcattgc tctcgataag gcgggtattt cctatgatga tgagctcctg aagggacaga
     4561 atgctcttgc attttcttat ctggatgcag tacaaatagc taaattatta gctgatgctg
     4621 gtaaaaagct gcctcaactg gagatcaaag ggggttggct agaccgtaat cctttaaaac
     4681 ctgaagaagt taaacgttta gccgaacttc cttctcggga gcaactaatt gctcaagtag
     4741 tgggaggaat gaaagcccct attgctggtt tggtgggagt tttgcaagga ttgttgcaaa
     4801 acttggtggg agttttacac gctattgaag agaaaaagaa tgcttagagg gaggattaat
     4861 catgactaaa gaagaaatta ttgaatccat tgagaaaatg actgttttgg aattggccga
     4921 actggtgaaa gcgctggagg agaaatttgg agtttcagca gctatgcccg tagctcaggt
     4981 agcagcagga ggagcgcagg gacctgctac tcctgaagta gaagagaaaa ctgagtttga
     5041 tgtggtattg aaaagcgtag gagaacagaa acttcaagtt atcaaggaag tacgggctat
     5101 tactggttta ggtttgaagg aagccaaaga actggtagat aatgctcctc gctcggttaa
     5161 agaggcggtt agtaaaaaag aagccgaaga aattaaagct aaattagaag cagtaggtgc
     5221 agaaatagaa attaaataaa aagaggagcc ttaaggctcc tcttttttaa aactaccttg
     5281 aataaaaaat atctcttaaa tccttatttt ctgttaatcc tcgtttctct catctgggga
     5341 tttaatttca gcgtggtcaa aataggaatt ctgaccatcg gtcctctggc tttttctctt
     5401 ttacgtttca tattgagtgc tttgctaatg tttttggttc tatggcttac ggaaaagaat
     5461 ccctgggtag aggtaaaaga ccttccttat tttttgtttc tgggtctttt aggattcggg
     5521 atttatcagc ctctgtggag ctatggactt aaactcagtt tggcttctca ttcggctctt
     5581 attttgtctc tttctccaat tgtggtatct ctggtggctt ttgtgcgaaa agaagagctg
     5641 gtgggaggaa taaacttatt aggggtaata ataggttttg gaggagtcat atttttagta
     5701 ggtcaaggag gaaatgggga ttatgctcca cagtctgttt tatatggaga cttattgact
     5761 ttaggggctg ccatttgctg ggggctttac tcttattttg gcaaaaacat ggtcaagaaa
     5821 tattctcctc ttaaaacttc cacctggagt gtcatctggg gattggtgtt tatgttcccg
     5881 gtgtgtatag gagaaactct tcaggtaaca agggaagact ttagtttttc tcttttgctt
     5941 tctctttctt actctgtagt cctatcttca ttagtagctt atatcatctg gatgcaatgt
     6001 gtaaaagaac ttggagcagc gcgtactatt tcttctcagt atattactca ggtagtaggc
     6061 gtaattgctg cctggatgat atttggtgaa cctttgggat ggcgagtatt tttggggatg
     6121 gctttcatca gcctgggttt gtggttaatt cataaaaaac cctcgggggg agaattaatc
     6181 ttataagcct aatttcccgg aaaattataa ctatgttttt gtatcaatcc ccagactctc
     6241 cataattgat gctgagattt ggtaatctct gtgagaatgt gcccatactt accagagtaa
     6301 cgaatttgag tcaatgtctc catctccaag agtaactccc ggactgtgta cttctcagtt
     6361 aagccggtat cccgcatctt tttccgcaag gcactcaggt tagcctttat tccgataaac
     6421 ccgcagggct tccaccggat ctttgatgtc attggtaaga agcacataga aaccagcata
     6481 ccggttacgg tatttctgaa tggcttcttg gttaaaaagc actttgcgac cccgtactgg
     6541 ggtctcctta atggtgaaga aggtctggta agcatcggca tggttactca ctaattgccc
     6601 ggtctccaac tctgttttgt aggtcagtaa atcttccata aagctatcag tgacttttgc
     6661 tccaggctct gcagtgagag agggggccac ctctcgtggt gatatagtaa gccatgctca
     6721 aggagttata atcaaaagtt gtgtatggcc aaatgaaaac aggtggcgat tctcgg
//
LOCUS       DAOSVE010000091         6772 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_5434_length_6772_cov_70.050618, whole genome shotgun sequence.
ACCESSION   DAOSVE010000091 DAOSVE010000000
VERSION     DAOSVE010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 6772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6772
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5434_length_6772_cov_70.050618"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..870)
                     /locus_tag="PK016_07280"
     CDS             complement(4..870)
                     /locus_tag="PK016_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HXL02897.1"
                     /translation="MPLVSTKTILKLAEEKNFALPAFNVPIPEFILWILEEGEEMRSP
                     LIIQVAPVEYEALDIRMFYGTLLFLSQKFSIPFSLHLDHAHRWEEILLAIQNGFSSVM
                     IDGSRLPLEENINLTNKVIEIAHPVGVLVEGELGRVGGLEGDESWEEKSKEGDFFTSP
                     EEAEEFISKAPVDMLAVAVGTRHGLYEGRTPQLQFKRIKEIREKSKLPLVIHGGSGTP
                     REQLQKAAQLGVRKINFSTALRVQYLSAFPEYLKNHPQELMVGKVLKGVEGKVKEAIK
                     ECIVSSCAEGKV"
     gene            complement(1097..>1651)
                     /locus_tag="PK016_07285"
     CDS             complement(1097..>1651)
                     /locus_tag="PK016_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02898.1"
                     /translation="YLERAKTKARFIAYPNRSIMISPWAIKDAKDRIISLEIYMKHEL
                     SHILLYQNMGFIADLRYPKWLLEGIAMYTSNQLGTSLYPSKSQVYELIQKGNHFPPQF
                     YETSQKDDIQLDVENRVTFMYSEFACLVDFLIERYGREKFHQYMTGLMVNKNHDNLFK
                     KIYNISFTDFQDEFIEHVKNNAFK"
     assembly_gap    1652..1751
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2571..3509
                     /locus_tag="PK016_07290"
     CDS             2571..3509
                     /locus_tag="PK016_07290"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HXL02899.1"
                     /translation="MANILMLFSRLQKAGLEVILPPYPHPLKEEELREFVSQVDGIIG
                     GLDPVTPSLLEEAKCLRVISKHGVGVDNIAVEEATRRGIVVANTPGANDDSVADLAFA
                     FVLSLARHIPRAYWSMKEGKWEKIVGIEIKGKTLGVIGTGRIGKKVICRSGGFEMRVL
                     AYDVAPDWDLSQKWQFSYVSLEELLRESDFVTLHIPLTRETKGFIGEKELSLMKKSAY
                     LINTARGGIVKEGALYRALRERMIAGAAWDVFYQEPLGNNPLLGLENVIATPHMASDT
                     EEAICRMDIVSAENVIRVLRGERPLSAVNMVEKVQR"
     gene            3521..4288
                     /locus_tag="PK016_07295"
     CDS             3521..4288
                     /locus_tag="PK016_07295"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HXL02900.1"
                     /translation="MNGKLLKNKLEAKKPCVGSWVTFTDPTITEILCGAGYDFLVIDT
                     EHAPLGVETVQLHLLATKGEDTVPIVRVLWSDLVYLKQVLDIGAPGVMIPMVHNKEEA
                     EKAISFCLYPPEGIRGFGPRRAAGYDRRIPEYIEKANEEVLIILQIEHFETVENLEEI
                     LKVKGLSAIFIGPWDLSGSLGLLGEINHPRVRKYVDIIIEKANNANIPVGIAASSKAE
                     DVVGWFKKGVEFVTAGTDYGLLSQACDSSVVSIKQLL"
     gene            complement(4324..5559)
                     /locus_tag="PK016_07300"
     CDS             complement(4324..5559)
                     /locus_tag="PK016_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HXL02901.1"
                     /translation="MPKIYILDVTNRDGVQTARLGLAKIEKTMINFFLNEMGVYQSEF
                     GFPTTRHETYYLNANLELAEMGALSPIRLSGWLRAVKKDVELAYQMVPGLKYLNLSIS
                     TSDQMIHSKFQEKMSPETVIKSMVEAVETAKACGAQGIGINAEDASRSDIDYLIEFAK
                     AGKEAGAERFRYCDTLGYDSPFTIYERIAKIGEAVQMPIELHCHNDLGMVVANSIAGA
                     KAAVDTGCDVFINTTINGIGERAGNADLVSVILAIKYASGMDGEKYQLDERINLKAAW
                     KTCKYASYAFRVPIPITQPGVGANAFAHASGIHADGVLKDSKNYELYDFGELGRGEPE
                     IIETGRQIVIGEYSGIKGFRNVYNQLEIEFKDEEEAREVLELARFANVEKQKPLTSDE
                     LRFIAQYPQQAKKILTLIP"
     gene            complement(5572..6759)
                     /locus_tag="PK016_07305"
     CDS             complement(5572..6759)
                     /locus_tag="PK016_07305"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HXL02902.1"
                     /translation="MQITERVKKIEPSATLSISARAKALKREGRDIISFSAGEPDFDT
                     PLAIKEEAKKAIDEGFTKYTPTSGTPELKEAISKKLERENGLFYTPQEIIVSCGAKHS
                     LFNAILTLCNPGDEVLLPKPYWVTYLEQIKLAEGKPVFLEPSPSIFTVSIEEIKKALT
                     PRTHLLIINNPSNPTGVVWEEEELEEVAQLAKEKDLIIISDEIYEHLAYDGAKIRSIA
                     AFPGMKDRTIVINGVSKTFSMTGWRIGYAAGPREVIEAMGRLQDHSTSNPTSISQRAA
                     WKALQSDSSLFEKMVKKFDERRKLITQHLSEIPSVTFPIPRGAFYVFANFSPYLGKSF
                     QGKPINNSLELAESLLEEAQIACVPGTAFGMEGYLRFSYAISEENIEKGMERLKNFVN
                     QMN"
BASE COUNT         1840 a         1287 c         1383 g         2162 t
ORIGIN      
        1 gtattatact ttgccctcag cgcaggagga aactatacac tcttttatcg cttctttcac
       61 ctttccttct actcccttta gaaccttgcc caccataagt tcttggggat gattctttaa
      121 gtattctggg aaagcactta aatattgcac tctcaaagca gtgctgaaat ttatctttct
      181 cactcctaat tgtgccgctt tttgtaattg ttcccgaggt gtgccacttc ctccatgtat
      241 tacaaggggt aatttacttt tttctctaat ttctttaatg cgtttaaatt gaagctgggg
      301 tgttctccct tcgtataatc catggcgagt tcccactgct acggctaaca tatctaccgg
      361 tgctttagaa atgaattctt ctgcttcctc gggggaggtg aaaaagtctc cttctttact
      421 tttttcttcc cagctttcgt ctccttctaa tcctcctact cttcccaact ctccttctac
      481 taataccccc actgggtggg caatttctat tactttgttg gttagattaa tattttcctc
      541 taaaggtaaa cgagagccat caatcatcac tgacgagaaa ccattttgta tagctaagag
      601 aatttcttcc catcgatgag cgtggtctaa gtggagagag aaaggtatag aaaatttctg
      661 ggagagaaaa agcaatgtcc cgtaaaacat ccttatatcc agggcttcat attctaccgg
      721 agctacctgg ataataaggg gagaacgcat ttcttctcct tcttccagaa tccataaaat
      781 aaattctggg atgggaacat tgaaagcagg aagagcaaag tttttctctt cagctaattt
      841 tagaatagtt ttagtagaga ctaaaggcat aataaagctc ctttcttttt ggctattttg
      901 aaattatagc agatttttcc tcctttcctc ggaaatattt tctcgatgct caggagaaat
      961 atcgttatgg tgaggggggg gtagaaactt tccgtctata cgctctctgt aaggaagaaa
     1021 gctaaaaact aaatattatg acagatgcaa acgaggtatt cttaataaaa gtgagaactg
     1081 gaaaagaagg tacaaattac ttaaaggcgt tatttttcac atgttctatg aactcatcct
     1141 gaaaatcggt aaaactgatg ttataaatct tcttgaacaa attgtcatgg tttttattga
     1201 ccatgagccc ggtcatatat tggtggaatt tttctcttcc atatctttcg attaaaaaat
     1261 cgactaggca agcaaattca gaatacataa atgtaactct attctcaaca tccagttgga
     1321 tgtcatcttt ctggctggtt tcgtagaact ggggagggaa atgattgcct ttttgaatca
     1381 attcataaac ttgactttta ctgggataca atgaagttcc taactgatta gaagtataca
     1441 tagctatacc ttccagaagc cattttggat atctgaggtc ggcaataaat cccatatttt
     1501 gatataaaag aatgtgggaa agctcatgtt tcatgtagat ttctaaagaa atgattctat
     1561 cttttgcatc tttaattgcc cagggggata tcattatact tctatttgga taagcaatga
     1621 atcgagcctt agtttttgct ctttctaaat annnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn naattgatat aaaagctaaa gtatatttcc gcttcatctt ctattcacta
     1801 tattgcttaa tagatttttt gttcctcttt gaataatatt tctgaataaa gctcgataat
     1861 cttttttata ttttccataa cctcttcaaa ggtttccccc ggcttggcat cctttaagtt
     1921 ctggacatta tgcataataa ctgtagtcac cttccttctc gtaattttac tgttgtttta
     1981 agcgggcgag ttgaccgttc tttttggatc ttccccaatt tatttcctcc ggagaggaat
     2041 ctttttaatg attaaagaga aaatattccg tgtcatgaaa gccatatgct tttcttttaa
     2101 tcctattata gtatcccgca aaacctgata taattgctaa agtttactaa aaatctcaaa
     2161 ggcgagatcg ccacgggaag cgagcaaacg accctcgcga tgacaagttg aggcaagatt
     2221 gctacattac ttcttacgat aataaaaggg aaatggcaat cccttccaga cggtcattgc
     2281 gagttcgtcg ttacgtttac gtggcaatcc cgagttttta acctctgatg taattggcct
     2341 ttttagtatt caccaaggat tcaaaagcga gattgcttca gagcttcctc ctcgcaaaga
     2401 caggaggggg tgagatctct cttgacagtt agttttacaa taggttttgc ggttaactat
     2461 attacagttt caactaagat gaatagctga gttttgtttt tgcttggtaa gaataaagga
     2521 aagggtaaat atggagaaat ataaagtatt agtcactcca actgcttctt ttggcaaata
     2581 ttctgatgct tttttctcgc ttgcaaaagg caggattaga ggtgattctt cctccctatc
     2641 ctcatccttt aaaggaagaa gagttaaggg agtttgtttc tcaagtagat ggtattattg
     2701 ggggcttgga ccctgtcact ccttctcttc ttgaggaggc taaatgtctc cgggtgatct
     2761 ctaagcatgg ggtaggggtg gataacatcg ccgtagaaga agcaactcgc cgggggatag
     2821 tggtagctaa tactccgggg gctaatgatg actcagtggc tgaccttgca tttgcttttg
     2881 ttctttctct tgctcgtcat attccccgag cttattggag catgaaagag ggaaaatggg
     2941 aaaaaattgt aggaatagag attaaaggaa aaactttagg agtgattgga actggccgga
     3001 ttgggaaaaa agtaatctgc cggtcggggg ggtttgagat gagagtgctg gcttatgatg
     3061 tggctcctga ctgggaccta tcccagaagt ggcagttttc ttatgtatca ttggaagagt
     3121 tactgcgaga aagtgatttt gttactctgc atattcctct tactcgggaa accaagggtt
     3181 ttataggaga gaaagagctt tctctaatga agaaaagtgc ttatcttata aatactgctc
     3241 gtggaggaat tgtgaaggaa ggagcccttt atcgagcgct aagagagaga atgattgctg
     3301 gagcggcttg ggatgttttt tatcaggagc cactaggtaa taatcctctc ttaggtttag
     3361 aaaacgttat tgctactcct catatggcct ctgatactga agaagctata tgccggatgg
     3421 atatagtgtc agcagaaaac gtcattcggg tgctccgggg agagagacct ctttcggcag
     3481 taaatatggt agaaaaagta caaagatgag gaggtttatt atgaatggga aactattaaa
     3541 aaataagttg gaagcaaaaa agccttgcgt gggcagctgg gtaaccttta ctgaccctac
     3601 aattactgaa attttatgtg gagcaggcta tgattttttg gtcattgata ctgagcatgc
     3661 tcctctggga gtggagacgg tacaacttca ccttttggct accaaagggg aagatactgt
     3721 ccccatagtg cgagtgctat ggagtgattt agtttatttg aaacaggttc tggatatagg
     3781 agcgcctgga gttatgattc ccatggtaca taataaagaa gaggcggaaa aggcaatctc
     3841 tttttgtctt tatcctccag aagggattag aggcttcggc cctcgacgag cagcgggtta
     3901 tgaccgccgg atacctgagt atattgaaaa agctaatgag gaagttttaa tcattctgca
     3961 aattgagcat tttgagaccg tggagaattt ggaagaaatt cttaaagtaa agggattaag
     4021 tgcaattttt attggtccct gggatttatc gggttcttta ggtttattgg gagaaattaa
     4081 tcaccctcga gtgagaaaat atgttgatat cattattgaa aaggccaata atgctaacat
     4141 accggtaggg attgccgctt cttctaaagc agaagatgtg gtagggtggt ttaaaaaagg
     4201 agtagaattc gttacagccg ggacagacta tggtcttctt tctcaagctt gtgatagctc
     4261 agtagtttcc ataaagcaat tgttgtaaat ttctaaggaa gggtgggttt atccaccctt
     4321 cctttaagga atcaaagtaa gaattttttt agcctgttgg ggatattgag cgatgaaccg
     4381 caactcatcc gaggttaacg gtttttgttt ttctacgtta gcgaaacgag ctaattctaa
     4441 tacttctcgt gcttcctctt catctttgaa ttctatttct aattgattgt aaacgttgcg
     4501 aaatcccttt atcccgctat attcgccgat tacaatctgt cttcccgttt ctatgatttc
     4561 cggttctcct cttcctaact ctccaaagtc ataaagctca tagtttttgc tatcttttaa
     4621 tactccatcg gcatgaattc ccgaggcatg agcaaaagcg ttggctccta ctccgggttg
     4681 agtgatgggg atgggaactc ggaaagcata agaagcatac ttgcaagttt tccaggccgc
     4741 ttttaaattt attcgctcat ccagttgata tttttctcca tccatgccgc tggcatattt
     4801 tatggctaaa attactgaaa ccaagtcagc attccctgct cgctctccta tcccattgat
     4861 ggtagtgtta ataaaaacat cgcatcctgt gtccactgct gctttcgctc cggcaataga
     4921 gttagccact accatgccta agtcattgtg acagtggagc tcaatgggca tctgaaccgc
     4981 ctctccaatt ttagctatac gctcataaat ggtaaaaggg ctatcataac ctaaagtatc
     5041 gcaataccgg aagcgttctg ctcctgcttc tttacctgct ttggcgaatt cgattaaata
     5101 atctatatca gaacgagaag catcttcagc gtttattccg attccttgtg ctccacaggc
     5161 cttggcagtt tctactgctt ctaccataga ttttattact gtttctgggc tcatcttttc
     5221 ttgaaattta ctatgaatca tctggtcgga ggtagaaata gagagattga gatatttcaa
     5281 ccctggaacc atctggtaag ctaattcaac gtcctttttt accgctctca accatccact
     5341 caatcttatg gggctcagag ctcccatttc tgctaattct aaattggcat tgaggtaata
     5401 agtttcatga cgggtggtag gaaaaccgaa ttcgctttgg taaaccccca tttcgttgag
     5461 gaaaaagttt atcatagttt tttcaatttt agctaaccct aaacgagcag tctgtactcc
     5521 atcacgattg gtaacatcca gaatatagat tttaggcata tacacacctc ctcaattcat
     5581 ttgattgaca aagtttttta atcgctccat tcctttttca atgttttctt ctgaaatagc
     5641 ataggaaaag cgaaggtaac cttccatccc gaaagctgta cccgggacac aagctatttg
     5701 ggcttcttct aaaagagatt cagctaactc taaagagtta ttaatgggtt tcccctggaa
     5761 gcttttacct aaataagggg aaaaattggc aaaaacgtaa aaagctcccc gaggaatagg
     5821 aaaagtgact gagggaattt ctgaaaggtg ttgtgttatg agttttctcc gctcgtcaaa
     5881 ctttttcacc attttttcga atagagaaga atcgctctgt aaagctttcc aggctgctct
     5941 ctggcttata gaagtgggat tagaagtaga atggtcctga agccttccca ttgcttcaat
     6001 tacttcccga ggtccggcag catatccaat tcgccagccg gtcatggaga aagttttgga
     6061 cacaccattg attactatag ttctatcttt cattcccgga aaagcagcta tgcttcttat
     6121 ttttgctcca tcataagcga ggtgctcata tatttcatca gaaattatta ttaaatcctt
     6181 ttctttagcc aattgcgcta cttcttctaa ctcttcttct tcccatacta cccctgtggg
     6241 gtttgaaggg ttgtttatga taagaagatg agtgcgagga gtgagagctt ttttaatttc
     6301 ttcgatactc acagtaaata tagatgggga aggctcaaga aaaactggtt ttccttcggc
     6361 caattttatc tgttctaagt aggtaaccca gtaaggtttg ggcaaaagca cctcatctcc
     6421 agggttacaa agggtcagaa tagcattaaa aagagagtgc tttgcgccac aggaaactat
     6481 tatttcttga ggggtgtaga aaagcccgtt ttctctctct aattttttac tgatagcttc
     6541 ttttagctct ggtgttccag aagtaggggt atatttggtg aatccttcat cgatagcttt
     6601 tttagcctct tctttgattg ccagaggagt atcaaaatct ggttcaccgg cactgaagga
     6661 aattatatcc cttccttccc tttttagagc cttggctcga gcagaaatag atagagtagc
     6721 ggaaggctcg atttttttaa ctctttctgt tatttgcaaa atatcacctc ct
//
LOCUS       DAOSVE010000092         6649 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_5537_length_6649_cov_66.612223, whole genome shotgun sequence.
ACCESSION   DAOSVE010000092 DAOSVE010000000
VERSION     DAOSVE010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 6649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6649
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5537_length_6649_cov_66.612223"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            383..583
                     /locus_tag="PK016_07310"
     CDS             383..583
                     /locus_tag="PK016_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HXL02903.1"
                     /translation="MATGKVKWFSATKGYGFIESDEGGDVFVHYSAIEGSGFKTLEQG
                     QAVEFEVQQGDKGPQAVKVKRL"
     gene            complement(763..2211)
                     /locus_tag="PK016_07315"
     CDS             complement(763..2211)
                     /locus_tag="PK016_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXL02904.1"
                     /translation="MSYLDSFKAFTTEQVVKLMVNSLSNASDENIIKLTYLAEKLTPI
                     EYYRDQIRGLRQMFEEKRPQIILARRVLQETHPNVREKLVVNFIVKNILLGVPKRQKL
                     EKELGVQVPFFFVVSPSMRCNLNCYGCYAGQYRKESDMPIELMDRLFNEAKEMGMNFL
                     TISGGEPFIRKEHLDLFEKHNDISFQIYTNGTLINREVAKRLAQMGNVYPAISVEGYE
                     EETDNRRGKGTFKKIMQAMEALRDEGVLFGFSITATRHNTELVSSDEFIEFLINKGCA
                     FGWFFTYVPIGKKPDTSLMPTPEQRLHLRSQVHRIRYEKPIFIGDFWNDGPYVGGCIA
                     GGRRYFHINNAGDVEPCVFCHFTVDNVKDKSLKEVIASPFFRAIQERQPYDNNLMRPC
                     MLIDVPEVLREVVEKYGARPSHEGAESLITTLKDEIDQYSQAFKKLTDPIWETDYKGT
                     VYYKDYKSERKEYLRKLEEAATNKEKVKAEVN"
     gene            complement(2259..2864)
                     /locus_tag="PK016_07320"
     CDS             complement(2259..2864)
                     /locus_tag="PK016_07320"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HXL02905.1"
                     /translation="MIKSLTGKEELLKEIKTEVEVCTLCPLSQSRTKIVFGEGDPDAL
                     LMFIGEAPGEEEDRTGRPFVGKAGQLLTKILQSVDLKREEVYIANMVKCRPPGNRTPT
                     QSEMDSCFPYLEAQIAIINPSIIITLGSTSTLHLLGKKEPISKLRGQWFDWRGGKKIF
                     PMFHPSFLLRYDSRQPGSPRYLTWKDIQEVKRTYDLLKQSS"
     gene            3417..5231
                     /gene="glmS"
                     /locus_tag="PK016_07325"
     CDS             3417..5231
                     /gene="glmS"
                     /locus_tag="PK016_07325"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HXL02906.1"
                     /translation="MCGIIGYVGENPALPFLLQGLKKLEYRGYDSSGVALLEGENILA
                     FRKKGKITELEKDLSGKEFASICGIGHTRWATHGIPSERNAHPHVDCHRNIALVHNGI
                     IENYRQLREELEKEGHRFSSETDSEVIAHLLEGEDNPLKRILGVVKKIQGSYALAIIF
                     KEEPGVVYGVRKSSPLILGIGDKSNFLASDIPAFLEYTRKAVFLEDEEIIRITPREWE
                     VFDFAGAKKGKEIFPISWDPVSAEKGGFKHFMLKEIHEQPQVLEDTMAGRINLLKGKV
                     FLEEIENLPLSFSRIVIVACGTAYHAGLLGKIYLEKFTRLPAEVDYASEFRYRDPLVD
                     ENTLVICISQSGETADTLAAADLARRKGARVLSICNVMGSSLTRLSQDVIYTRAGVEI
                     GVASTKAFLCQEAVLLLLALYLGDKRRMISQKELLTLLQEMTTIPRHIRSILEESDFI
                     HQLAREFYLFRDFLYLGRGLFYPLSLEGALKLKEISYLRAEGLSSGEMKHGPIALVDP
                     EVVSVVLVGKGVTKPKIIGNIEEIKARQGKIIALVLGRDEEVEEKVDRITSIPDVEET
                     LSPLLAIVPLQLLAYYIALERGCDIDQPRNLAKSVTVE"
     gene            complement(5232..6143)
                     /locus_tag="PK016_07330"
     CDS             complement(5232..6143)
                     /locus_tag="PK016_07330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HXL02907.1"
                     /translation="MREQKLSSEEISSNPLASKAYYAKLEAWASILVNFILFGIKLYY
                     GNLSQSLALTADAFETLSDVATSLAIFFIARELNRAPDREHPFGHGRLEDTTTLIIAV
                     LLFVVGIQFLIQGYRRILHPSPIQFSLPALFAILIGIGGKEILAQIALFLGKKASNSE
                     LLIAKAWHHRSDALDSLPVLVVFLFPRYPWLDGVSSLFTSITIIWIASKILRGSVSRL
                     LGRSISPQEREKILNIASQFKEVSNVHSIYLHDYGGRGVITMHIEVDGNLSVKEGHDL
                     SEKMEKEIKNQTGFEGIVHVEPFSEEK"
     gene            6329..>6649
                     /locus_tag="PK016_07335"
     CDS             6329..>6649
                     /locus_tag="PK016_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02908.1"
                     /translation="MVREIGKRFQTEVDSEAGMVNIFVLVMVSFLLIASSWLLSDVFL
                     HYQVVNAHIKKFKEEYQIEGLLVEAIQLLKNGAFQENHENYISSSFNSLFRFSIKEDK
                     LIISK"
BASE COUNT         2022 a         1195 c         1341 g         2091 t
ORIGIN      
        1 ttctcgtgct attttagctt ttagtgctgc gtcatacttc tttcggatgt cacccatttt
       61 cttatctttt tcctttccat tttacaggtt aaatacacct tattactctg tccagttttg
      121 ggggtccatt atagactaag ggaaacatgg gcttttcttc tttttttcag ttgtttaaga
      181 ttatctctta taaaatctaa taataacttt ctctctcttt ctctaagaac tttaatactt
      241 accaggggga taccatcttc cttccgtgtt ccaaaataaa agcaaggggt cattgacaaa
      301 accggaaagt gatgatacta tgctctccga gcttgcgcaa gcttattaat tttcaattta
      361 tttttgggag gtttaagttt aaatggcaac tggcaaagtt aaatggttta gtgccaccaa
      421 aggttatgga ttcattgaat ctgacgaagg tggggatgtt ttcgttcact actcggctat
      481 cgaaggtagc ggctttaaaa ctttagagca aggccaagca gtagagtttg aggtacagca
      541 aggtgataaa ggacctcaag cagtaaaagt aaagagacta taggaaaaaa gggaaaaaaa
      601 gccctgggga aatttttccc cagggctttt ttgttgggct acgtcagcat agacgatagg
      661 ttttgagtga aatgattttt gaggatggat cttaagccaa aaacaaaaat cccagagcag
      721 gaattcctgc tctgggattc accttaaaat aaagcctgtt ttttagttta cttctgcctt
      781 tactttttcc ttatttgtgg cagcttcttc taacttacgg agatattctt ttctttctga
      841 tttgtaatcc ttatagtata ctgttccttt gtagtcagtt tcccagatag ggtcggttaa
      901 ttttttgaat gcctgagagt attggtctat ttcgtcttta agagtagtta tcaggctttc
      961 tgctccttca tggctgggac gagctccata tttctccact acttctcgca aaacttcagg
     1021 aacatcgata agcatgcacg gtctcatcaa gttgttatca taaggctgtc tttcttggat
     1081 agctcggaag aagggagaag caataacttc ttttagagat ttgtctttta cattatccac
     1141 ggtaaagtga cagaacacgc aaggttctac atccccagcg ttattaatgt ggaaatatct
     1201 tcttcctcca gcgatacaac ctcctacata tggtccatca ttccagaaat cgccaataaa
     1261 aattggcttt tcataacgaa tgcggtgtac ttgactgcgg agatgaagtc tttgttctgg
     1321 agtaggcatt agggaagtat ctggtttttt acctatggga acgtaagtaa aaaaccatcc
     1381 aaaagcacac cctttgttga ttagaaattc tataaactca tcgctggata ccaattcagt
     1441 gttgtgtcga gtagcagtaa tggagaagcc aaagagtact ccctcatctc gcagagcttc
     1501 catagcctgc attatttttt taaatgtgcc ttttcctcgg cgattatcag tttcttcttc
     1561 gtagccttcc acacttatag cgggatagac attacccatt tgagctaagc gtttagccac
     1621 ctctcggttg attaaagtac cattagtgta aatctggaaa gatatatcgt tgtgtttctc
     1681 aaaaaggtct aaatgttctt ttctgataaa tggttctcct ccagaaatag tgagaaaatt
     1741 catgcccatt tccttagctt cgttaaataa acgatccata agctcaatgg gcatatcgct
     1801 ttctttacga tactgtccag cataacaacc gtagcaatta aggttacatc tcatagaagg
     1861 gcttactaca aagaaaaagg gaacttgcac gcctaattct ttttccaact tttgcctttt
     1921 gggtactcct aaaagaatat tcttgacgat aaagttaact acaagttttt ctctcacgtt
     1981 gggatgagtt tcctgcagaa cacggcgggc taatattatc tgaggccttt tctcttcaaa
     2041 catttgacgg agccctcgaa tttggtctcg ataatactcg ataggggtaa gcttctccgc
     2101 caaataggtg agcttgatga tgttttcatc agaagcatta cttaaagaat ttaccattag
     2161 ctttactacc tgttcagtag tgaatgcttt gaaagaatct aaataactca tattaagtcc
     2221 ctccctcaaa aggaattttt tttattataa cacattattt aagagctttg tttcagaagg
     2281 tcatatgttc ttttaacctc ttgaatatct ttccaggtta aataacgggg agaccctggt
     2341 tgtcgggaat cataacggag tagaaaactg ggatggaaca tggggaagat tttttttcct
     2401 cctcgccagt caaaccattg accacggagt ttactgatag gttctttttt acccaaaagg
     2461 tgaagagtag aggtactacc taaagttatg ataatagaag gattaatgat agcaatttga
     2521 gcctccaggt aaggaaaaca actatccatt tcgctttgag taggggttct attgcccgga
     2581 gggcgacatt ttaccatatt ggcaatatat acttcttctc tttttaaatc tactgattga
     2641 aggattttgg tgagcaactg gccggcttta cctacaaaag gtcttccagt ccggtcttct
     2701 tcttccccgg gtgcttctcc gataaacatg agcaacgcat cagggtctcc ctctccaaaa
     2761 actattttag ttctgctttg agaaaggggg caaagggtac acacttctac ctcagttttt
     2821 atctctttta ataattcttc tttgccggtc aaggacttta tcacttgtca cctacaaaat
     2881 aaagaataca ttcttatttt caactattat tattaattct atcatataat taaacttttc
     2941 gaaaaccttt tccgatgcct tcgtgtttta ttaagtcaag gagtattctt tgccaggtaa
     3001 gtacagcccc tccgtataag ggcttataaa agaactataa agtgattttt ccttacaacc
     3061 ctttaagaaa aggaaaaagt atcatcacac agttgatact atgaagctaa tataatcttg
     3121 tgtaaatgtt agctaagaaa agatgtgaga ctcacaatgg ctcaagatat gaactaaaat
     3181 acaaaaactg aaatagttac attagaaaaa cagaataatc tgtgacacaa aaaagcataa
     3241 aaaaataaag attactgaga gaagaaagaa ctttcttcag tatttgcggt aagaaacaga
     3301 gttaaacatc tagaaagaaa tattcgtgat gcttttgtga ttaatataat tttagactct
     3361 tggataatag gaagacgttc agctataatt attataggac gtaggggtga gtagaattgt
     3421 gtggaattat aggctatgtg ggagaaaacc ccgctttgcc atttcttctc caagggctta
     3481 aaaaattaga atatcgaggt tatgattctt ccggggttgc ccttttagaa ggagagaaca
     3541 ttcttgcttt tagaaaaaaa ggtaaaatta ctgaattaga gaaagattta agcgggaaag
     3601 aattcgcctc tatttgtgga atagggcata ctcgttgggc tacccacggg attccctcag
     3661 aaagaaatgc tcatcctcat gttgattgcc atagaaatat tgctttggta cacaatggca
     3721 taattgaaaa ttatcgccag ctcagagagg aattagaaaa agaaggacat cgattttctt
     3781 ccgaaactga tagcgaagtt atcgcccatc ttttggaagg ggaagacaat cccctaaagc
     3841 ggattctagg ggtggtaaag aaaattcaag gttcatatgc cctggctatc atctttaagg
     3901 aagaaccggg agtggtttat ggagtgagaa aatctagccc cttaattttg ggaataggag
     3961 ataaaagtaa tttcttagcc tctgatattc cagctttttt agaatatacg aggaaggcgg
     4021 ttttcttgga agatgaggaa ataataagaa tcactcctcg agaatgggaa gtttttgatt
     4081 ttgcgggagc aaaaaagggt aaagagattt ttcctatctc ctgggatccg gtaagcgcag
     4141 aaaagggtgg atttaaacat tttatgctca aagaaattca tgagcaaccc caagttttag
     4201 aagatactat ggcaggaaga atcaaccttc taaaaggaaa ggtcttcttg gaagaaatag
     4261 aaaatttacc actttctttt tcccgaatag taatagtagc ctgtggaaca gcatatcatg
     4321 ccggtttatt gggtaaaatt tatctggaga aatttactcg ccttcctgca gaagtagatt
     4381 atgcctctga atttcgctat cgtgatcctc tggtagatga aaataccttg gtgatatgta
     4441 tctcccaatc aggagagaca gctgatactt tagcagcagc agaccttgcc cgtcgcaagg
     4501 gagcaagagt tctttctata tgcaatgtga tgggtagctc tcttactcgt ttgtctcagg
     4561 atgtaattta tactcgagca ggggtggaaa ttggagttgc ttctaccaaa gcttttctct
     4621 gccaggaagc ggttctcttg cttttagcac tttatttggg ggataaaaga aggatgattt
     4681 ctcagaagga gttactaact cttttgcaag agatgaccac cattcctcgc catatccgat
     4741 ccattttaga ggaaagtgat tttatccatc agttagctcg agaattctac ctgtttcgag
     4801 attttctcta tctgggaagg gggctttttt atcctctatc tttggaaggg gctttaaaat
     4861 taaaagagat atcctatctc cgggcagaag gtttaagttc tggagagatg aagcatggtc
     4921 ctatcgcttt agtggatcca gaagtagtga gtgtagtttt ggtaggaaaa ggagtaacta
     4981 aaccaaaaat tatcggtaat atagaggaaa taaaagctcg tcaagggaaa ataatcgcct
     5041 tagtactggg aagagatgaa gaagtggaag aaaaagtaga tagaataact tctattcctg
     5101 atgtggagga aacacttagc ccgcttttgg ccatcgttcc tctgcagcta ttggcctatt
     5161 atatagcttt agagagagga tgtgacatag accaacctcg taatttagct aaaagtgtaa
     5221 cagtagaata gtcatttttc ttcgctaaaa ggctccacat gaactatccc ttcaaagcct
     5281 gtttggtttt taatttcctt ttccattttt tctgacaggt catgtccttc tttaactgag
     5341 aggtttccat ctacctcgat atgcatagta ataacccctc tacctccata atcatgaaga
     5401 taaatggaat ggacattgct tacttcctta aattgggaag caatatttaa gattttttcc
     5461 ctttcctgag gggaaatact ccttcctagt agccgggaaa ctgaaccccg taagatttta
     5521 ctggctatcc agatgatagt aatagaggta aataaactgc taactccatc taaccaggga
     5581 tagcgaggaa acaaaaatac cactaaaacg ggcagggaat ctaaagcatc actacggtgg
     5641 tgccaggcct tagcaatgag gagctcagaa ttagatgctt ttttccccaa aaaaagagct
     5701 atctgggcta aaatttcttt tccccctata cctatcagta tggcaaagag tgctggtaaa
     5761 gaaaattgaa taggagaagg gtgaagaatc ctccgatagc cttggattaa gaattgaata
     5821 cccacaacaa aaagaaggac ggctatgata agggtagtag tatcctccaa tcgaccatgt
     5881 ccgaagggat gttctcggtc tggagcacga ttgagctctc gggcgatgaa gaaaatggct
     5941 aaagaagtag ctacatcaga taaagtctca aaagcatcag cagtaagagc taaactttga
     6001 gaaaggtttc catagtaaag ttttatccca aaaaggataa aattcactaa tatactggcc
     6061 caagcttcta atttggcgta ataagcttta gaagccaaag gattactaga aatttcttct
     6121 gaagaaagtt tttgctctct cacttttcac tctttgattt tcggtaaaat aatggtggcc
     6181 tttttttact tttttatatt ataatccttt aggcttcatt gtaaagttat tgaaaatagt
     6241 ggtcttttct ctgtataaca tcaagaaaaa taaaggagat taatttattg acttccatta
     6301 actttgtttt taaaggggag gataaagaat ggtcagagag ataggaaaga gattccaaac
     6361 cgaagttgac tcagaagcag gcatggtaaa tatctttgtc ctggtgatgg ttagcttttt
     6421 acttattgct tcttcctggc ttcttagtga cgtgtttctt cattatcaag tagtaaatgc
     6481 tcatattaaa aagtttaagg aggaatatca gatagaggga ttgttagtag aagctatcca
     6541 attgttgaaa aatggtgctt tccaggaaaa tcatgaaaat tatatttctt cctcttttaa
     6601 ctctcttttt cgcttttcaa taaaagagga taaactgatt atcagtaaa
//
LOCUS       DAOSVE010000093         6244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_5872_length_6244_cov_67.651640, whole genome shotgun sequence.
ACCESSION   DAOSVE010000093 DAOSVE010000000
VERSION     DAOSVE010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 6244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6244
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5872_length_6244_cov_67.651640"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..753)
                     /locus_tag="PK016_07340"
     CDS             complement(49..753)
                     /locus_tag="PK016_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HXL02909.1"
                     /translation="MNLNLIGDLHIHTVASGHGYSTVMEIVQVAKRKKLEVVAITDHS
                     PKMPGGPSPIYFEARGHFPREIEGIKIYFGAEVDILDEEGSLSLEDKLLKKLDIVIIS
                     FHPQVFPPASREINTQALLRALENPAVDIIAHPGNPRYPLDYERVVKEAVSRGKVIEI
                     NNSSFAVSRKGSKENCRLIAQEVKKQGGWVVVSSDAHFCEEVGEFKEAQDLLEEVGFP
                     PDRVLNSSRDNIEKFL"
     gene            complement(758..1930)
                     /locus_tag="PK016_07345"
     CDS             complement(758..1930)
                     /locus_tag="PK016_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02910.1"
                     /translation="MAKRKKSLVFSGGKNSNFFSQQGEIELLEKLPLLLREEEVILEQ
                     FFSFYRAVLREKKNLIFLSSPAVISFLQNFLMGEAASRGEQFIGFVDSSRPDLLLCWG
                     EKLPPQDTTLVVLARPLEELQLLLLAFSFPQWRKVLVGNNYGILAQSARVLFLPFLPT
                     EIYHHYFWQRSALFYLPLISLRLPVEELEEGFKNSDYLQREAAAVSIFIQDVEKKGIR
                     RIIFLVDNSFILSILQEFLPLLEYSCRGGGNMEFCAFNFSIMRDGQLFWPDDSVFILV
                     ESRKTDKIKFTLPPALKKIDLFSGEGIIPASGSWKKIKEIEYQVVENWLQNEGKGFLK
                     WEYYLPDLQTVGELMTFVRYLVSYNAWLHGLDLWSDPPVVEFDNQIWRLLSSNRKR"
     gene            complement(1906..3492)
                     /gene="cimA"
                     /locus_tag="PK016_07350"
     CDS             complement(1906..3492)
                     /gene="cimA"
                     /locus_tag="PK016_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HXL02911.1"
                     /translation="MPAEVEIYDTTLRDGSQAEGINFSVQDKLQIAQKLDELGIDYIE
                     GGWPGSNPKDIAFFQEVKKISFKKAKISAFGSTRKPSIRVEEDKNIRSLVEAQTPVVT
                     IFGKSWTLHVREALKTTLDENLHMIEDSVSFLVKQGREVIYDAEHFFDGFKDNSDYAL
                     KTLKVAYEAGAQILVLCDTNGGTLPLEMEAIIGRMKEFFGKDREIKWGIHAHNDSGVA
                     VANSLIAVQQGAHQVQGTINGYGERCGNANLCTIIPNLKLKMNIHPITDEEMRKLREV
                     SYFVSEIANLKPNDYDPYVGKSAFTHKGGIHVSAISRYPATYEHASPESVGNERRILV
                     SEQAGRSNLIYRARELGIELDSQDPRLLNLVQRIKEAENYGYQFEAADASFELMLREA
                     LGERAEFFDLEGFRVIVEKRGEEDTVAEATIKVKVDEKMIHTAAEGDGPVHALDNALR
                     KALEEMYPVLKEIRLSDYKVRVLNEKEGTGALIRVLIQSTDGRSSWGTVGVSADIIEA
                     SWQALTDSIKYGLWRRERKV"
     gene            complement(3514..4269)
                     /locus_tag="PK016_07355"
     CDS             complement(3514..4269)
                     /locus_tag="PK016_07355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HXL02912.1"
                     /translation="MDLDGVIYLLNQPIPENIRFVNFLRENHFKVVFLSNNTFLSRRG
                     YVRKLRKMGIKAREDEIFSSAFVTAHYLRGKKPDAKIYVIGEAGLKREIENCGLHIVP
                     DDTEKVDFVVVGMDRKFNFKKMSMALNLILSGGELIGSNPDVTYPTESGILPGCGAIL
                     AGIETCSGRKAQVIGKPSPIILDFVFHYTGFDREETVIIGDRLDTDVKLAKDLGLFSI
                     LVLTGVSKKEDLSRFTAQPDMVVENLEVLKEFF"
     gene            complement(4530..5765)
                     /locus_tag="PK016_07360"
     CDS             complement(4530..5765)
                     /locus_tag="PK016_07360"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXL02913.1"
                     /translation="MSDYSAELFLTLLESPKIDTHEHLLPDEEFLKSPDILLITLERS
                     YLSWILSGTTGKTFNFTREEFLSGVDTIPASSFYRYLIRAFQFMYGFSDDSLTLENWD
                     SLSQQIRSDYEKGERLRRWLQEKLNVQRIILDRYWVIGDFEVDRSIFLPTLRLDPFFF
                     GYNREGRDHDGQSPYFYSDREGIEVETFSDYLNLIDHILCKGKREGLGALKCAIAYDR
                     GLAFEKVPREKAERAFYRKTGEETREEITSFQDFIFHYFLQKAGELNLPLQIHTGPGK
                     AFQAVPSFLGPIFEEYREVKISLFHGGFPWIGEPGAMAIFYPHLYLDLVWLPLLSPTF
                     AGLALREWLEATGGSRIMMGGDSWNVEGAVGSIIYNLETISRVLGEMVEDGYLAKNTA
                     LELGKRILWDNPQEFFTSL"
BASE COUNT         1913 a         1482 c          986 g         1863 t
ORIGIN      
        1 taatttactt tttaaaagtt ttaatatttt aaccagtttt gcgagatact ataaaaattt
       61 ttctatatta tctctactgg agttgagtac ccggtcagga ggaaaaccta cttcctcgag
      121 caagtcctgg gcttctttga actctcccac ctcttcacaa aaatgggcat cactcgatac
      181 tactacccac cctccttgtt tttttacctc ctgggcaatc aagcgacaat tttctttact
      241 gccttttcta cttacagcaa aagaactgtt attaatttct ataaccttcc cccgactaac
      301 tgcttctttt accactcttt cgtaatccag agggtaacga gggtttccag ggtgagcaat
      361 aatatctacc gctggatttt ccagagcccg tagtaatgct tgagtgttta tttctcgtga
      421 ggcaggagga aaaacttgag gatgaaaaga aataattaca atatccagct ttttcagcaa
      481 cttatcctcc agacttaaac ttccttcttc atctaatata tccacctctg ctccaaaata
      541 aatttttatc ccttctattt cccgaggaaa atggcctcga gcctcaaaat aaataggaga
      601 aggaccgccc ggcatcttgg gactgtggtc agttattgct actacctcca atttttttct
      661 ctttgctacc tgtactattt ccatcaccgt actatatcca tgaccactgg ctactgtatg
      721 gatgtgcagg tctcctatta aattcaggtt catcacctca ccgttttcta ttagaagaaa
      781 gtaacctcca tatttgatta tcaaattcca ctaccggcgg gtcactccat aaatctaaac
      841 catgtaacca ggcgttatag gaaacaagat atcgtacaaa tgtcattagc tcccctactg
      901 tctggaggtc aggtaaataa tattcccatt tcagaaaacc ttttccttca ttttgcaacc
      961 aattctctac tacctggtac tctatctctt ttattttttt ccaggaacca gaggcaggaa
     1021 ttatcccctc cccggaaaaa aggtctattt ttttgagtgc aggaggaaga gtaaatttaa
     1081 ttttatcagt cttcctactc tccaccagaa taaaaacaga atcatcaggc cagaataatt
     1141 ggccatctcg cataatagaa aaattaaaag cacagaattc catatttcct cctcctcgac
     1201 aactgtattc taaaagagga agaaattcct gcagaatgga aagaataaag ctattatcta
     1261 ccaaaaaaat aatccttctt attccctttt tctccacgtc ctgaataaaa atagaaacgg
     1321 ctgccgcctc tctttgaaga taatcgctat ttttaaatcc ttcctccaat tcttctactg
     1381 gcaagcgaag gctgattaaa ggtaaataaa aaagagcgct cctctgccag aaatagtggt
     1441 gataaatttc agtaggcaag aaagggagaa aaagaactcg agcgctctga gccagtatgc
     1501 cataattatt ccctaccagc acttttctcc actggggaaa agaaaaagcc aaaagtaaaa
     1561 gctgcagctc ttccagcggt cgagctaaaa ccacaagggt agtatcctga ggaggtaatt
     1621 tttcccccca gcagagaaga aggtcaggcc ttgaactgtc gacaaagcct ataaattgtt
     1681 cccctcggga ggctgcctcc cccattagaa aattctggag aaaagaaatt actgcaggag
     1741 aagacaaaaa aatgaggttt ttcttctccc ttaaaactgc tcgataaaaa ctgaagaatt
     1801 gttctaaaat tacctcttcc tctctaagaa gaagaggtaa tttttctaaa agctcgattt
     1861 ccccttgctg agaaaaaaaa ttgctgtttt tcccgccgga aaagactaaa cttttctttc
     1921 tcttcgccat agtccatatt ttatgctatc agtaagtgct tgccaactgg cctcaattat
     1981 atcggcagat acccccactg tcccccagga gcttcttcca tcagtagatt gaatcaaaac
     2041 ccggataaga gctccggttc cttctttttc attcaaaact cgtaccttat aatctgacaa
     2101 tcgaatttct ttcaataccg gatacatctc ttccagcgct ttccttaaag cattatccag
     2161 agcgtgaacc ggaccatcac cttctgctgc tgtgtgtatc atcttttcat ccactttcac
     2221 tttaatagtg gcttcggcca ccgtatcttc ttctcctctt ttttccacaa ttactctaaa
     2281 accttccaaa tcgaaaaact cagctctttc tcctaaagct tcccgcaaca taagttcaaa
     2341 agaggcatct gctgcttcaa actgatagcc atagttttct gcttctttaa ttctctgcac
     2401 caggttaagc aggcgagggt cttgagagtc taattctatt cccaactctc tagctcggta
     2461 aattagattg cttcttcctg cctgctccga taccagaatt ctcctctcgt tacccactga
     2521 ttcaggagaa gcatgctcat aagtagcagg ataacgagaa atagcactaa catgaatccc
     2581 tcctttatga gtgaaagcac ttttccctac ataaggatca taatcattag gttttaaatt
     2641 agctatctca ctgacaaaat aggaaacctc cctcagcttt cgcatttcct catcagtgat
     2701 gggatggata ttcatcttta attttaaatt aggaattatg gtgcaaagat tggcattccc
     2761 gcatctttcc ccatacccat tgatagtacc ctgcacctga tgtgctccct gctgcactgc
     2821 tatcaagcta ttagctaccg ccactccact atcattatga gcatgaatac cccattttat
     2881 ttccctgtct ttgccaaaaa actccttcat tcgaccgatg attgcttcca tctctaaagg
     2941 aagagtccca ccgttggtat cacacaaaac taaaatctgt gctcctgcct cgtaagctac
     3001 tttaagagtt ttcagagcat agtctgagtt atctttaaat ccatcaaaaa agtgttccgc
     3061 atcataaatt acttctcttc cctgtttcac taaaaaactc accgaatctt ctatcatatg
     3121 taagttttca tccagggtag ttttaagtgc ttcacgaaca tgaagagtcc aggacttacc
     3181 aaaaatagta accactggag tctgggcttc cactaaagag cgaatatttt tatcttcttc
     3241 tactcgaatg ctaggttttc tcgtactacc aaaagcacta attttagctt ttttaaagga
     3301 tatcttctta acttcctgga aaaaagcaat atccttgggg ttagaacctg gccaaccccc
     3361 ttctatgtaa tcaatcccca attcgtccaa tttttgagca atttgcagtt tatcctgtac
     3421 tgaaaaattg attccttcgg cctgtgagcc atcccgtaaa gtagtatcgt aaatctcaac
     3481 ttccgctggc attatatcac ctcgccacca taactaaaaa aattccttta aaacttctaa
     3541 attttccacc accatatcag gttgagcagt aaaacggcta aggtcttctt ttttacttac
     3601 tccagtcaaa actaaaatag aaaagagacc caaatcttta gctaacttta catcggtatc
     3661 tagacggtct cctataatta ccgtttcttc tctatcaaat ccagtgtagt gaaatacaaa
     3721 gtcaagaata ataggagagg gcttaccgat aacttgagct ttcctccccg aacaggtttc
     3781 tatccctgcc agaattgccc cacaacctgg taaaatgcca ctctcagtag gataagttac
     3841 atcaggatta ctaccaataa gctcgccacc agataagatg aggttgagag ccatgctcat
     3901 ttttttaaaa ttaaattttc tgtccattcc taccactaca aaatctactt tttcagtatc
     3961 atcaggaact atgtgcaagc cacaattttc tatctctctt ttcaatcctg cttccccgat
     4021 cacataaatt ttcgcatcag gcttttttcc ccttaagtaa tgagcggtaa caaaagcgga
     4081 actaaaaatc tcgtcttctc tcgccttaat ccccattttc ctcaactttc tgacataacc
     4141 tcttctactc aaaaaagtat tattggataa gaaaaccact ttaaagtggt tttctctcaa
     4201 aaagttaaca aaacgtatat tttcgggaat aggttgattg agaagataaa ttactccatc
     4261 aaggtctatg ataaaatttt taaaattttt tagtggcttc tgaattattt taaatcctgc
     4321 ctttcacttg aattaagcat ttttaaacaa tttacttatg aaatgaattc tattgttcaa
     4381 ttttattctg tattttaaac ttcttcctat attatcacaa gagacgaaat taaattaagg
     4441 tcggttaaga aatgaaaaac cccagactat cctttataat tcccaacatt tttaaaaatg
     4501 gagatatttt caacccctaa aaactctctt tatagagaag taaaaaattc ttggggatta
     4561 tcccacagga ttctcttccc taactctaaa gcagtgtttt tagccaggta tccatcttca
     4621 accatttccc ccaaaacccg ggatatggtt tccaggttat agataataga ccccactgct
     4681 ccctctacat tccaggaatc tcctcccatc attatccggc ttcctccggt agcttctaac
     4741 cactctctca aagccagacc agcaaaagta ggagataaaa gaggaagcca gaccagatcc
     4801 agataaagat ggggataaaa aatagccata gctcccggtt ctcctatcca ggggaatccc
     4861 ccatgaaaga gagaaatttt tacttctcga tattcttcaa aaataggtcc caaaaaagag
     4921 ggaacagcct gaaaagcctt gcccgggccg gtgtgaatct gtaatgggag gttcagctct
     4981 ccagcttttt ggaggaaata gtggaaaata aaatcttgaa aactggtgat ttcttctctg
     5041 gtctcttctc ccgtttttcg ataaaaagcc cgctccgctt tttcccgagg cactttttca
     5101 aaagctaatc cccggtcata agcaatagca cactttaaag ccccgagtcc ttcccttttt
     5161 cctttacaga ggatatggtc aataagattc aggtaatcac tgaaagtctc tacctcgatt
     5221 ccttcccggt cagagtaaaa ataagggctc tgaccatcat ggtctcggcc ttctcggttg
     5281 tagccaaaaa agaaagggtc aaggcgtaaa gttggaagga aaatacttct atcaacctca
     5341 aaatcaccta ttacccagta gcggtccaga ataattctct ggacgtttaa cttttcttgc
     5401 aaccatcttc ttaacctctc acctttttca taatcacttc taatttgctg agaaagactg
     5461 tcccaatttt ccagagtcag agaatcatcc gaaaacccgt acataaactg aaaggcccga
     5521 ataagataac gataaaaaga actggccggg atagtatcta ccccggataa aaactcttcc
     5581 ctggtaaagt taaaagtttt tccagttgtc ccggacaaaa tccaggataa ataacttcgt
     5641 tccaaagtaa taagaagtat atccgggctt tttaaaaatt cttcgtcagg tagaagatgt
     5701 tcgtgagtat caattttggg actttccaaa agagtaagaa ataactctgc agaataatcg
     5761 ctcattctta aaccctcttt ctaaagaaaa aataactgac gcctcttaat tatagtatcc
     5821 cgcaaaactg gttcttgaca ataagaggga aacaacctcc cttagtttct atttcctgct
     5881 gtacttttta acttccgctg tgctgcattt ctattctacc aaacacaccc cttatctatc
     5941 atcccctcag ccgtcattcc tgaatggttc tatcaggaat ccaaacaatg agggggcaag
     6001 ccccctcata accccaaaaa agctaaagga aaaagacaaa aacttaaaaa ataagcaaaa
     6061 attactggtg ggaaaatatc ttccttagtg ggacaaagcc cccttataat cgaactataa
     6121 gggggtaaac ccccttcata acccccaaaa agattgaaaa acaaataaaa aagcaaagac
     6181 aactgaggaa aacgcgtttc cttcctcagt ggatgcctga taaaagcact caggcatgac
     6241 agaa
//
LOCUS       DAOSVE010000094         6192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_5927_length_6192_cov_70.065341, whole genome shotgun sequence.
ACCESSION   DAOSVE010000094 DAOSVE010000000
VERSION     DAOSVE010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 6192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6192
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5927_length_6192_cov_70.065341"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PK016_07365"
     CDS             complement(<1..489)
                     /locus_tag="PK016_07365"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02914.1"
                     /translation="MARTNINREGTSTLDMQIKPIPETDLISYLPKITSFILPKEFEL
                     LPNIAEIFELEFAFYEYKFLTRDEIIHRGAFFKSVDGIPTYHYIICSNSSPLIWKGRS
                     ELTKSYFKEGNFSTGYATHGLFPYRGKFHPQLIKGILNMLKIQPGDIILDPMCGSGTL
                     NVE"
     gene            complement(594..1490)
                     /gene="whiA"
                     /locus_tag="PK016_07370"
     CDS             complement(594..1490)
                     /gene="whiA"
                     /locus_tag="PK016_07370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HXL02915.1"
                     /translation="MDFRTFLKDEIYAQIPSSPRMCRGEWWGIFRGGGAIKRREGNSY
                     LSFSHTDLSLIKKCLWLKNKLYPHLPHLLLQEEKRGINAGKWYSLDILWEESFWEEGG
                     FSDLSVVQDMLKGKVAGAFLRGLFEVRGYISDPLRHHHLEIGPLSPDLASVVLGYLEK
                     KDLPFQLRERKKESYIYTKNSSVIGSFLHYLGASQAYLQWEKLAIEKSTLNNLTRWVN
                     WETANLERTVNSSLRQRQKLQEIDFDSLPSRLQEVARLRLEHPYASLREIGNLCSPPL
                     SKSGAYHRLREIEKIIEEQGKR"
     gene            complement(1480..3294)
                     /gene="uvrC"
                     /locus_tag="PK016_07375"
     CDS             complement(1480..3294)
                     /gene="uvrC"
                     /locus_tag="PK016_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HXL02916.1"
                     /translation="MTRRSYTRFEVKKEDWNKLPPECGVYIFRNSGGEIIYVGKAKNI
                     FKRVSSHLHHRYSSPVKRDMVEEIDTIGYFLVPDEHQAFLLEDELIKTHQPRYNLRLK
                     DDKSYPYLVFSTEGDFPAVKMARRKTTPFGQFFGPFTNVKKVREGLKLLRSLFLLRGC
                     AISESRFPLDRPCLDFELGLCCAPCLGSVSKEEYRERVKKAQEFLNGDYEGVLSWLEE
                     EMWKKAEELDFEEAARHRDRLKAVNEILARYQLVLSQPEDIDFIDIASDEDLASLIVL
                     RVRKGRLIGSESFLTSGGEREEILREFGEEFYVNRFNLPSRVCWEGGKLPEIEEEYNV
                     VFTPPLSEEEKEIMTFAQSNAQRNLEVEKLKMKKRERISEKVQEELQALLGIPTPPEW
                     VEGVDISTFQGGETVGAIVSFRGGLPDKNNYRKFIIRETDYPNDYECLREVLERHLRS
                     LQEKSSPFPDLLLVDGGIGQLNVALEVLEKLGVKFPVLSLAKEWEEIYLPGKSSPLRL
                     PPHSEALQFLQRVRNEAHRFAITFHRLRRNKQSFGSILEEVKGIGPKRKKKLLSAFND
                     LREILEADSREVSKNLGIPEKTIEEVKKQLEKNLSRGF"
     gene            complement(3260..6073)
                     /gene="uvrA"
                     /locus_tag="PK016_07380"
     CDS             complement(3260..6073)
                     /gene="uvrA"
                     /locus_tag="PK016_07380"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HXL02917.1"
                     /translation="MNLDGKIIVKGAREHNLKNVDLEIPRNRFIVITGLSGSGKSSLA
                     FDTIYAEGQRRYLESLSSYARQFLERMEKPDVDYIEGLSPAIAINQKAASHNPRSTVG
                     TVTEIYDYMRVLFARCGKAFCPECGKPITRQTVPQITDEILSLPSGTRVMILAPLVRG
                     RKGEYRKLLGDMLKNGFARVIVNGEMIELEDWESVELDRNKKHEIDLVVDRIVVEKDA
                     ARRISDSVEIALRYGRGVVKVRIQEGEEDRDVFFSEHFACVDCGISFPEITPRMFSFN
                     NPYGACPDCSGLGFLSFVDPALVVPDESKSLREGAVEPWGELDSDSYSGERIIKFLKS
                     HEISLNKPFRKLKEEEKDLIFYGDGGFEGVINYLERKLRSAAGWWEEEEISPYLSTRT
                     CPTCRGDRLRKESLSVKIQNFSIGDLSRLTIAELRSFFSHLEFTGSELVIAQPIMREI
                     LSRLTFLEEVGLDYLNLNRPAATLSGGEAQRIRLATQIGSGLVGVLYVLDEPTIGLHP
                     RDNEKLISTLKNLRDLGNTVLVVEHDADTIRNADFIVDMGPGAGEKGGEIIARGSLLE
                     IMNDPHSITGLYLSGKREVPLPRERKKPGEEWLVVKGAREHNLKNITVEVPLGLLVGI
                     TGVSGSGKSTLMDEVLYRSLARVFYRSRWEAGEHDSIEGVEFLDKVVVIDQSPIGRTP
                     RSNPATYTGVFTEIRNLFAKLPESRARGYRPGRFSFNVKGGRCEACQGNGTIKIEMHF
                     LPDVFVTCEHCKGKRYARETLDIKYKGKNIAEVLDLTVNEALSFFENIPAIRRKLEIL
                     DRVGLGYIRLGQSATTLSGGEAQRIKLARELSKIATGRTLYLLDEPTTGLHFADIEKL
                     LDVLLELRSRGNTILIIEHNLDVIKSCDYLIDLGPEGGERGGYLIAKGTPEEVAENPA
                     SYTGQFLRRVLSLDQEKLYSVRS"
BASE COUNT         1755 a         1543 c         1095 g         1799 t
ORIGIN      
        1 ctctacattt agagtcccac taccgcacat agggtcaaga ataatatctc caggttgaat
       61 ctttagcatg ttcaatatac cctttatcaa ttgtggatga aatttccctc gatatggaaa
      121 aagtccatgt gtagcatacc ctgtggaaaa gttaccttct ttgaagtatg acttggtcag
      181 ctctgacctt cctttccata tgagaggact gctgtttgaa caaattatat aatgatacgt
      241 agggattcca tcaacacttt tgaaaaatgc ccctctgtga atgatttcat ctctggtaag
      301 gaatttatat tcatagaaag cgaattctaa ttcaaagatt tcggcaatgt taggcagtag
      361 ttcaaattct ttcggtaata taaatgaggt tattttaggt aaatacgata tcaagtcagt
      421 ttccggaatt ggtttaatct gcatatccaa agtagaagtt ccctccctat ttatatttgt
      481 cctggccacg taccaatctc cttaatcctc tgctaaccca ggcaacacca ggctctaaat
      541 tttagtgaat tctttcccat atactactca accggaacaa gatggtcaag cgattatctt
      601 ttcccctgct cttctattat tttttcaatc tctcgcaggc gatgataagc tccagattta
      661 gaaagaggag gagaacaaag gttgccgatt tcccgaagag aagcataagg gtgctctaat
      721 cgcaggcggg cgacttcctg aagacgagaa ggtaaagaat caaagtcaat ctcctgaagt
      781 ttctggcgct gacgcaggga ggagttcact gttctctcca gattagcggt ttcccagttt
      841 acccatcggg tgaggttatt gagagtagat ttttctatgg ccaacttttc ccactgcaga
      901 taagcttgag aagctcctag gtagtgcaag aaagagccaa taacagaaga atttttggta
      961 taaatataac tttctttttt tcgttcccgg agttgaaaag gtaaatcttt tttctccaga
     1021 tatccaagaa ccaccgaagc taaatcagga gaaagagggc caatttctaa atgatgatga
     1081 cgtaaagggt cggagatata accccggact tcaaataacc ctcgtaaaaa agcgccggcg
     1141 actttcccct taagcatatc ttgcactaca gaaaggtcag aaaatcctcc ctcttcccag
     1201 aaactttcct cccacaggat atctaaagaa taccattttc cggcattaat ccctcttttt
     1261 tcttcttgta agagaagatg gggaagatga ggataaagtt tattcttcag ccacaagcat
     1321 ttttttatca aactcaaatc agtatgagag aaggaaagat aactattacc ttctctccgt
     1381 ttaatcgctc ctcctccccg gaatatcccc caccactccc ctcggcacat cctgggggag
     1441 gaaggaatct gagcgtaaat ttcatctttg agaaaagttc taaaatccac gagataagtt
     1501 tttctccagt tgttttttaa cttcttctat agttttttcg ggaataccca ggtttttact
     1561 aacttcccga gagtcagcct ctaaaatctc tcttaaatca ttaaaggcag aaagaagttt
     1621 tttcttcctc tttggtccta ttcctttcac ctcttctaaa atcgaaccaa aagattgttt
     1681 attacgccgc aaacggtgaa aagtgatggc aaagcgatga gcttcgtttc tgactctctg
     1741 caaaaattgt aaagcctcag aatgaggagg taaacgcaaa ggggaagatt ttccgggaag
     1801 atatatttct tcccactcct tggctaagga tagaaccgga aacttaactc ccagtttctc
     1861 taaaacctct aaagccacat taagctgccc tatccctcca tctacaagaa gcaaatcggg
     1921 aaagggagaa gatttttctt gaagagaacg gagatgcctt tctaaaactt ctcttaaaca
     1981 ttcataatcg ttgggataat cagtctctcg aatgatgaat ttccgatagt tatttttatc
     2041 cggtagccct cccctaaagg aaactatggc tcctaccgtc tctcctccct gaaaagtgga
     2101 aatatccact ccctctaccc actccggagg ggttggaata cccaaaagag cttgcagctc
     2161 ttcctggact ttttctgaaa tcctttctct ctttttcatt ttcagctttt ctacttctaa
     2221 atttctctgc gcgttacttt gagcaaaagt catgatttct ttttcttctt cgctgagggg
     2281 aggagtaaaa actacgttat actcttcttc tatctcgggc aattttcccc cttcccaaca
     2341 tacccgagaa ggaagattaa acctattaac ataaaattct tccccaaatt ctctcaatat
     2401 ctcctctctt tccccacctg aggtaaggaa actctcactc cctatcaagc gccctttcct
     2461 cacccggagc acaatcaaag aagctagatc ttcatcgctc gctatatcga taaaatctat
     2521 atcctccggt tgagaaagaa ccagctgata ccgagccaga atttcattaa ccgctttcaa
     2581 tctatcccga tgccgggctg cttcctcaaa atccagctct tctgccttct tccacatctc
     2641 ctcttctaac caggagagca ctccctcata gtcaccattc aaaaattctt gcgctttctt
     2701 tactctctct ctatattcct ctttactcac tgaaccaaga caaggagcac agcagagccc
     2761 cagctcaaaa tcgagacacg gcctatctaa gggaaaacgg ctctccgaaa tagcacaacc
     2821 ccgcaagaga aaaagagaac gcagaagttt caacccctcc ctcactttct ttacattagt
     2881 aaaggggcca aaaaactggc caaaaggagt agtttttcgc cgggccattt tcactgccgg
     2941 gaaatctcct tcagtagaaa aaaccaaata agggtaactt ttatcgtctt ttaagcgcag
     3001 gttatagcga ggctggtgag ttttaattaa ttcatcttct aaaagaaaag cctggtgttc
     3061 atcgggaacc agaaaataac caatggtatc tatctcctcc accatatccc tttttacagg
     3121 agaagaataa cgatggtgaa ggtgagagct cacccgctta aaaatattct tagctttacc
     3181 cacatagata atttcccccc cggagtttct aaagatatac accccgcact ccgggggcaa
     3241 tttattccag tcttctttct taacttcgaa ccgagtatag cttctcctgg tcaagggaaa
     3301 gcactctcct caaaaactgc ccggtatagg aagcaggatt ttctgctact tcctcaggag
     3361 ttcctttagc tatcagatat ccccctcttt ctcctccttc cggtcctaaa tcaatcaagt
     3421 agtcgcagct ttttatcaca tctaaattat gctcaatgat taaaatcgta tttcctcgac
     3481 tgcgcaattc cagtaacaca tccagcagtt tttcaatatc ggcaaaatga aggccggtag
     3541 ttggttcatc taaaagatac agagttctac cagtggctat tttactcaat tctcgagcta
     3601 actttattct ctgcgcttct cctcccgaaa gagtggtagc cgattgcccc agccggatat
     3661 aacctaaccc cacccggtct aaaatttcta attttctccg aatagccgga atgttttcaa
     3721 agaaagaaag agcttcattt accgtgaggt ctaaaacttc agcaatgttt ttacctttgt
     3781 atttaatatc taaggtctcc cgagcatatc ttttaccttt acagtgttca caagtaacaa
     3841 acacatcggg gagaaagtgc atctctattt taatcgtccc attaccctgg caggcttcgc
     3901 accttcctcc tttaacatta aagctaaagc gccccggacg ataacctcga gcccgggatt
     3961 caggaagttt ggcaaagaga ttcctgattt cggtgaaaac tccggtatag gtagcagggt
     4021 tggaacgtgg agttctacca atgggggact ggtcaataac caccacttta tccaggaact
     4081 ctaccccttc tatagaatca tgttcccccg cttcccaacg agaacgataa aaaacccggg
     4141 ctaaagaccg atacaaaact tcatccatca aggtgctttt tcccgaacca gagacgccgg
     4201 tgattcccac caaaagcccg agaggaacct ctacagtgat atttttcaaa ttatgctccc
     4261 gggctccttt aactaccagc cactcttctc ctggtttctt tctctctcga ggtagaggca
     4321 cttctctttt tccgctcaaa tacagaccgg ttatactatg agggtcattc attatctcta
     4381 aaagagaacc tcgggctatt atttctcctc ctttttctcc tgctcccggc cccatatcca
     4441 cgataaaatc agcatttctg atagtatcgg catcatgttc cactactaaa actgtgttac
     4501 ctaaatctcg cagatttttt aaagtagaaa tcagtttttc attatcccgg ggatgcaggc
     4561 caatggtggg ctcatctaaa acgtagagaa ctcccactaa acctgagcca atctgagtag
     4621 ccaaacggat tcgctgcgct tctcctcccg aaagagtagc agcaggacga ttaagattga
     4681 gataatcgag acccacttct tccaagaatg ttaaacgaga gagaatctct cgcattatgg
     4741 gctgagcaat taccagctca cttccagtaa attctaaatg agaaaaaaag gagcgcaatt
     4801 cagcaatggt cagacgagag aggtctccga tggaaaaatt ctggattttt accgacaaac
     4861 tctctttccg caggcggtct ccccggcagg taggacaggt tctcgtagat aagtaaggag
     4921 aaatttcttc ttcctcccac catcctgcag cagaacgcag ctttctctct aaataattga
     4981 tgactccctc aaatcctcca tcaccataga aaattaagtc tttctcttct tccttcagtt
     5041 ttcggaaggg cttattcaag cttatttcgt gggacttcaa aaactttata attctctctc
     5101 cactataact atcggagtcc aactctcccc agggttctac cgctccttcc ctcaggcttt
     5161 tcgactcatc aggaactaca agagcagggt caacaaaact cagaaacccc aatccactac
     5221 agtccgggca tgctccataa ggattattaa aagaaaacat ccggggggta atttcaggga
     5281 aactaattcc acagtccaca caggcaaaat gctcgctgaa gaagacatct ctatcttctt
     5341 ccccctcttg aattctcacc tttactacac ccctaccata gcggagagct atctctaccg
     5401 aatcacttat ccttcttgcg gcatcttttt ccactactat ccggtctacc actaaatcaa
     5461 tttcgtgttt tttatttcta tctaactcta cactttccca gtcttctaac tctatcatct
     5521 ctccatttac aatcactcga gcaaaaccat ttttcagcat atccccgagg agcttgcgat
     5581 attccccttt tcttcctcgc actaaaggtg ctaaaatcat cacccgggtg ccagaaggga
     5641 gagataagat ttcatcagtt atctgcggga cagtctggcg agtgataggt tttccacact
     5701 cgggacagaa agctttcccg cagcgagcaa ataaaactcg catataatcg taaatctcag
     5761 taactgtccc cacggtagag cgagggttgt gagaagcagc tttttgattt atggcaatag
     5821 ctggagataa accttcgata tagtccacat cgggcttttc cattctctct aaaaactgcc
     5881 gggcataaga agaaagagat tctaaatatc ttctctgccc ttcagcataa atggtgtcaa
     5941 aagccagaga agacttccct gaccccgata aaccagtaat aactataaag cgattacggg
     6001 ggatttctaa atccacattt ttaagattgt gttcccgtgc tcctttaact ataattttcc
     6061 catccaggtt catgttttat tcccaacttt caatcctttt cttttttctt taattacccg
     6121 gttaccagat tttatcttga gaatttcatc tctcaagata gctgcttttt caaactccaa
     6181 gtgacgtgct gc
//
LOCUS       DAOSVE010000095         6077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_6042_length_6077_cov_71.399369, whole genome shotgun sequence.
ACCESSION   DAOSVE010000095 DAOSVE010000000
VERSION     DAOSVE010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6042_length_6077_cov_71.399369"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..2048)
                     /locus_tag="PK016_07385"
     CDS             complement(114..2048)
                     /locus_tag="PK016_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXL02918.1"
                     /translation="MNEFIGTVTGLEEINPYCFFVRVNSSYSSNIALDQVVRVDYRWG
                     EREFHAYGMITEVKAKWDGTISTGYQEEAYNEGIYQGVPIYLGKVVVTRVLEKKGESL
                     IPVPYSFPPPAGEKVFGACGEELKVALGFSEIRKGKRALPCGILPSGDVAYLDLKYIL
                     GDNGAHINISGQSGVAAKTSYATFLLWMLMNYPHYVKDHVFSNQLANSRSLVFNVKGE
                     SLLFLDYWNEEWKKAQEKDPEGKRQFAEWQEMFRLFEVEPHPFSGLEVYVPPRNEKGS
                     PYIHHSRPEVKAYGWDILDIVELGLLPLMFDPEELSQNPNFLLTVTVIEDFLRDQYDR
                     LVEDARRELRSGSYPVKIALQGLTDQELVRIYLQAAGEEPRLILENFSLPNNLSSLIT
                     MLRLSLEGEGKLGEELQREQIDPRTTLAISRRLRAARRTGLNLLWRDITRLPYREVKE
                     VPEGGYHLRWNRPGGISVVDISKLTYQGQAFVVGAVLREIMHWKERELLAEPVFVYLD
                     ELNKYAPRTGGGPLGNIFRDVAERGRSFRVVLIGAEQTASQVDYRVITQAATTVVGRQ
                     KLAELHKEEYAHLEGNLRDKAATLLPGEVIIDQPFLRIPLTVKFPLPPWATAEENREE
                     LYREGVVFSPEEELKKLLGE"
     gene            complement(2041..3111)
                     /locus_tag="PK016_07390"
     CDS             complement(2041..3111)
                     /locus_tag="PK016_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02919.1"
                     /translation="MEEKRGSYLTLADFLRKKLAQVDHWFYEEDSFPRFFREENDSFL
                     TTSELWEIEEKDFASWPGKRPSPELEEVYLVDGRMRIHARIIVLMKTVLLGEVTAGFA
                     RWQKGKGLSMGFSPQNPPKLERVLGAPEGLFSGEMVSHELPLGPGFVFHVWESARTSR
                     YPQELEAAQQAIFSAMQVLEGKVVKDLLSHSSYIIKDGTLHEGDPEFQAGVGPCGLVK
                     RVEDLHLPSSYLESLFQLEKGERTPFFSAYLKKDSLTLRVFCYTRLVERDDNYPWKGL
                     VRLETLLNKERWIEEKDGLSLFFDGVVSLLPSLTADFPAKRLPENIFPVIALEEHLGQ
                     YLSSPPFVQSLIRKILGRKENE"
     gene            complement(3084..>6077)
                     /locus_tag="PK016_07395"
     CDS             complement(3084..>6077)
                     /locus_tag="PK016_07395"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HXL02920.1"
                     /translation="FGRGIRSERGKRELIHRGYPEGPLRVELEFLLGGNEFRVVREYS
                     PRGGAAVLEKKEDNTWRGLASGEQLVNQKIEETLGFDYLTFKSSVFLPQGETLSFVEA
                     TPAERFKTLSSLFGLELLDLIRERVKEELRSLERELLPREDRLKEREEENLEERLNLV
                     EKEKEDLEKRATVLTQEEEKKEEKTRLLGNLKDIEKNEKELQKRREDLEKEINIASQK
                     AREDREIQKAWEVKVSFWQPWRIVVERIKSWEEEKRTWLEKKTKIEEEITRLNKELKK
                     IRLQLEEQNTRRKNLESYEDKLRQEASPLAEEARAIKRRLQELEKDREERKEKIKEIE
                     KALQKERKEKEELEKKFVEEKEKTEREEEILQKVEKTKEKIEPLIRDLKEKEKEISLI
                     SEELKSLEEEIKTCSKGYEDLDNEWSKKRKELLLWQEEREIKEESYRQMVRDFALWEL
                     EKEWEEKGICPLCGSDVPPPSEIEAFPEIDWEGYHSFQEKGEKLLEEESSLRQKKIQE
                     EKKLTELKNKEREKREEQEKLKEEKGKILEALDSIDLPPLIEPEKIEEFLKKKREERE
                     KTLVNLYQLEKEKEVKKAKIENRIKEKDEEQEKIRNIERNIKEEEKLLAEKREQLSQI
                     FTLLRWEDSQPPEEVFNDLWGKFLKRLQVLSQELEKLGKEESRIEEKLKFLFQSKKEA
                     EEKIAQLLQGGEELQKEEREKKEAFQKELQRLEWTEEYFEQLRDKKAGNWQERWHRLE
                     GELKQVEKSLQLYEESKRNLIHQLGIEKDEVEELWEKEIEELEKIRGELGESKERKGS
                     LDKEIENLRDRIEERDKLREELQEKRKIWELHYQLDKALEARGFRSYLLGFLFRELEE
                     EASSFLEDLSQGRYFLRMKMEEGKAEMVVVDKNYGGEERLPSECSGGEKTLIALSLAL
                     ALSRIHLREVGKERGADCLFIDEGFSALDQEHLELVADAILRLSQDGRMVGIVTHDPS
                     FASYFPLHLEVKGGKAFWKRNEEVI"
BASE COUNT         1491 a         1540 c         1005 g         2041 t
ORIGIN      
        1 tctgtcattc ccgagcgctt taatcgggaa tccaagatcg cttttttctc tcccttaacg
       61 ggagaggatt aggttttcat cgtcatctgg tgtcacagca gtgacatgag ggtctattct
      121 cctaacaatt ttttaagctc ctcttcagga gaaaacacca ctccctcacg gtatagctct
      181 tctcgatttt cctctgcagt agcccaggga ggaagaggaa actttaccgt gagaggaata
      241 cgcaaaaaag gctggtcgat gattacctct ccgggtaaaa gagtggccgc tttatctcgt
      301 aaattccctt ctaaatgagc atactcttct ttgtgtagct cggctaactt ctgtctacct
      361 actactgtag ttgctgcttg agttatcact cgatagtcta cctgagaggc agtttgctct
      421 gcaccgatca aaaccacccg aaaagaacgt cctctctctg ctacatcccg gaaaatgtta
      481 cctaggggcc ctcccccggt acgaggagca tatttattga gttcatccag ataaacaaaa
      541 actggttcag ctaaaagctc tctttctttc cagtgcatta tctctcgcaa taccgctccc
      601 actacaaaag cctgtccctg gtaagtgagc ttagagatgt ctactactga tattcctcct
      661 ggacggttcc atcgcaagtg atagccacct tctggaactt ctttaacttc ccggtagggt
      721 aaacgagtga tatctctcca caaaaggttt aatccggtgc ggcgagcagc acgtaagcgg
      781 cgagaaatag ctaaagtcgt gcgagggtca atctgctctc tctgcaattc ctctcctaac
      841 tttccttctc cttctaaaga taagcgaagc atagtaatga gagagcttaa gttattgggt
      901 aaactaaaat tttccagaat aagccggggt tcctctcctg ctgcctggag ataaattcgc
      961 accagttcct ggtcggttaa tccctgtaaa gcaattttta ccggatagct accactgcgc
     1021 aattcccgcc gggcgtcctc caccaatcta tcatactgat ctcgtaagaa atcttcaatt
     1081 acagtaacag ttaaaaggaa attggggttc tgagagagtt cttcagggtc aaacatcaaa
     1141 ggtaaaagtc ccaattccac aatatctaag atatcccagc cataagcttt cacttctgga
     1201 cgactgtgat gaatataggg agaacctttt tcattgcgag gaggaacgta aacttccaat
     1261 cccgaaaaag gatgaggctc tacctcaaaa agacgaaaca tctcctgcca ctcagcaaat
     1321 tgtcttttcc cttctgggtc tttctcttgg gcttttttcc attcttcgtt ccagtaatcc
     1381 agaaagagga gactctcccc tttgacattg aagaccaaag aacggctgtt agccagctgg
     1441 ttagagaaaa catggtcctt aacataatgg gggtaattca tcaacatcca caacaaaaaa
     1501 gtggcataag aggttttggc tgccactcct gattgcccgc tgatattgat atgagctcca
     1561 ttatctccta atatgtattt aaggtctaaa taagccacgt ctccggaagg taaaatccca
     1621 caggggagag ctcttttccc ttttcggatt tctgaaaatc ccagagccac ttttaactct
     1681 tctccacaag ccccgaacac tttttctcct gcgggagggg gaaaagaata aggaacaggg
     1741 atgagacttt ctcctttttt ttctaaaact ctggtcacca ctactttccc caggtaaatg
     1801 ggcactcctt gataaattcc ttcgttataa gcttcttctt gataaccagt agaaatagta
     1861 ccgtcccact tagctttcac ctcggtaatc attccgtaag catggaattc tctttctccc
     1921 cagcgataat ctactctcac tacctggtcc agagcgatat tagaagaata agaagaatta
     1981 actcggacaa agaagcagta aggattaatt tcttctaagc cggtaactgt gccaataaac
     2041 tcattcattt tccttcctcc ccaaaatttt acgtatgaga ctctgcacaa atggaggaga
     2101 cgaaaggtat tgtcctaagt gctcctccag agcaattacg ggaaaaatgt tttctggtaa
     2161 tctcttggct ggaaaatcag cagtcaaaga gggtagaaga gacactactc catcgaaaaa
     2221 aagagaaagg ccatcttttt cctctatcca cctctcctta ttcaggagag tttctaaacg
     2281 taccagtcct ttccagggat aattatcatc tctttctact aagcgggtat agcaaaaaac
     2341 tcgtaatgtg agagagtcct ttttcaaata ggcactaaaa aaaggagttc gctctccttt
     2401 ttccaattgg aataaagatt ccaggtagga agaaggaaga tgtaaatctt ccactctttt
     2461 caccaagccg cagggtccca ctcccgcttg gaattccggg tctccttcgt gtagtgttcc
     2521 atcctttata atataggaag aatgagaaag caaatctttt accactttac cctccaaaac
     2581 ctgcatggcg ctgaaaatag cttgttgggc tgcttctaat tcttggggat agcgggaggt
     2641 gcgagccgat tcccagacgt ggaaaacaaa acccggccct aaggggagtt cgtgagaaac
     2701 catctccccg gaaaagagac cctctggcgc tcccaaaact ctttccagtt taggaggatt
     2761 ttgaggagag aaacccatag aaagaccctt acctttttgc caccgggcaa agccggctgt
     2821 tacttctcct aaaagcacag ttttcatcaa tactataatt cgggcatgga tgcgcattct
     2881 tccatctacc agatagacct cttctaactc tggagaggga cgctttcccg gccaggaagc
     2941 aaaatccttt tcctctatct cccacaattc agaagtggtt aagaaactat cattctcttc
     3001 ccggaaaaag cgagggaaag aatcttcttc ataaaaccag tggtcaacct gtgccaattt
     3061 ttttcgcaaa aaatcggcta aagttaaata acttcctcgt ttctcttcca aaaagccttt
     3121 cctcccttaa cctctaaatg cagcggaaaa tagctggcaa aagaggggtc gtgagttact
     3181 attcccacca tccgtccatc ctggctcaaa cgcaaaatag catcagctac cagctctaaa
     3241 tgttcctggt ctagagcaga gaaaccctca tcgatgaaaa ggcaatctgc acctctctct
     3301 ttacctacct cccggagatg aatacgagat agagccaggg ctaaagagag agcgatgagc
     3361 gtcttttctc cacctgaaca ttcactgggt aagcgttctt cccctccata atttttatcc
     3421 accaccacca tctccgcttt tccctcttcc atcttcatcc gtaagaaata acgcccctga
     3481 gataagtctt ctaaaaacga agaggcttct tcttccagct cccgaaagag aaaacctaaa
     3541 agatagctgc gaaaaccccg agcttctaag gctttatcca gctgatagtg aagctcccaa
     3601 atttttcttt tttcctgtaa ttcttctctc aatttatctc gctcttctat cctatctcgt
     3661 agattctcta tctctttatc cagacttccc tttctttctt tactttctcc cagttcccca
     3721 cgtattttct ctaactcttc tatctctttt tcccacaatt cttctacctc atctttttct
     3781 atccccaatt ggtggatgag atttctttta ctttcttcat aaagctggag agatttttcc
     3841 acttgcttta gctccccttc taacctgtgc cacctctcct gccaattacc cgctttctta
     3901 tcccgcaact gctcaaaata ttcttctgtc cactctaaac gttgaagctc tttctggaaa
     3961 gcttcttttt tttctctctc ctctttttgg agttcttctc ctccctgaag tagttgagct
     4021 atcttttctt ctgcttcttt cttactttga aagagaaact ttaatttttc ctcgattcta
     4081 ctttcttctt tccccagttt ctctaattct tgtgaaagaa cttgtagacg tttcaaaaac
     4141 tttccccaca agtcattaaa aacttcctct ggaggttggc tgtcctccca gcgtaaaaga
     4201 gtaaagattt gagaaagttg ctctcttttt tcggccaata atttttcttc ctcttttatg
     4261 ttcctttcta tattccttat tttttcttgt tcctcatctt tttcttttat tctgttctct
     4321 attttagctt tcttgacttc tttttctttt tccagttgat aaagattaac aagcgttttt
     4381 tctctctctt ctcttttttt cttcaaaaat tcttctatct tttctggctc tattagggga
     4441 ggaaggtcaa tactatctaa agcctctaaa attttgccct tttcttcctt caatttttcc
     4501 tgttcttctc ttttttctct ctccttattt tttagctctg ttaatttttt ctcctcctgg
     4561 atttttttct gacgaagaga actttcctct tctaataact tttctccttt ttcctgaaaa
     4621 gaatggtaac cttcccaatc tatctccggg aatgcttcta tctcagaagg tggagggaca
     4681 tcactaccac aaagaggaca aattcccttc tcttcccatt ctttttccag ctcccagaga
     4741 gcaaaatctc tcaccatctg gcgatagctt tcttctttta tctctctttc ttcttgccag
     4801 agcaatagct cttttctctt tttactccat tcgttatcta aatcttcata tcctttacta
     4861 caagtcttga tttcttcttc taagcttttc aactcctcgc tgataagaga aatttctttt
     4921 tctttctctt tcaaatccct gataagaggt tctatttttt cttttgtctt ttccactttc
     4981 tggagtattt cttcttctcg ctctgttttt tctttctctt ccacaaattt tttctctaac
     5041 tcttctttct cttttctttc cttttgcaga gccttttcta tctcttttat tttctctttt
     5101 ctttcctctc tatccttctc taactcctgt aatcttcttt ttatggctct cgcctcttca
     5161 gctaaaggag atgcttcttg gcgaagtttg tcttcatagc tttccagatt ctttcttctg
     5221 gtattttgct cttcaagctg gagtcgtatt ttttttaatt ccttattcag tcgagtaatc
     5281 tcttcttcta tttttgtttt cttttctaac caggttcttt tctcctcctc ccaactcttt
     5341 atcctttcca ccactattcg ccagggctgc cagaaagaaa ctttcacttc ccaggctttt
     5401 tgaatttccc gatcttcccg ggctttctga gaagctatgt ttatctcttt ttctaaatcc
     5461 tcgcgccttt tttgtaactc tttttcattt ttttctatat cttttaaatt acccagtaat
     5521 ctggtttttt cttctttttt ctcttcttct tgagttaaaa cggtcgccct tttttctaaa
     5581 tcctcttttt ctttttctac taagttcaag cgctcttcca ggttttcttc ttcccgctct
     5641 tttaatctat cttctcgagg aagaagttct ctttccaggg agcgcagctc ctcttttact
     5701 ctttcccgaa ttaaatccag cagttccaga ccaaagaggc tgctcaaagt tttaaatcgc
     5761 tcggcaggag tggcttctac aaaagaaaga gtttctccct gaggtagaaa cacggaagat
     5821 ttgaaagtga gataatcaaa acccagagtt tcttctattt tctggtttac cagttgctct
     5881 cctgaagcta aacctctcca ggtattatct tcttttttct ctaaaactgc tgctcctccc
     5941 cggggagaat attctctgac tactcgaaat tcattgcccc cgaggagaaa ttccagctcc
     6001 actcgcaagg ggccttcggg atatccccgg tgaattaatt ctcttttacc tcgctctgat
     6061 cgaatacctc gtccgaa
//
LOCUS       DAOSVE010000096         6060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_6059_length_6060_cov_76.415321, whole genome shotgun sequence.
ACCESSION   DAOSVE010000096 DAOSVE010000000
VERSION     DAOSVE010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 6060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6060
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6059_length_6060_cov_76.415321"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="PK016_07400"
     CDS             <1..1049
                     /locus_tag="PK016_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02921.1"
                     /translation="MINELFLQGIEAPWEEVDFRVGLLKMSWGASDIMSPVDVINPTP
                     FSLSFSENALENKIPVPALDLEWYHSQDWSWEFFYQPDFVPNFIPEGIEGQLLAYELA
                     SLLSVNPQKLVLNLTEEEPLVDFSHPIWGIRARGIVSNVDVAVSYLQSYYLSPYPRET
                     NVLMGLDGNSIVDVTLGYPKRSMLGLEFQGEFPLLEGSTFRGDIAIFMPERWVNHINI
                     TQPSGEVVSYPQEIFQNPYWKGSLGIDYTTDNNTLLNLVWMWGNPYEEGKDVSPYLFL
                     TAERPSEDNKWTTLLTSGVSLQDGSMVNVIGLTYKPKDNWNIALSYSFSSGAPGSKLG
                     EVGDGVFLNVKYSF"
     gene            1114..1704
                     /locus_tag="PK016_07405"
     CDS             1114..1704
                     /locus_tag="PK016_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXL02922.1"
                     /translation="MDKKEKILEAAKKVFAEKSFFEATLGDISLLSGIKKSTIYYYFG
                     SKLELLLEIVSVILQHATESIANIPSSLSSRETLERLIEGYFIFFYQERDSILVFRRV
                     GYDFFAHPEVFRELRQSFQKFRKVRENLGRRIGVLETKRGNKIDGTEIIRIILSSINA
                     YWIEEIVEGREIKLEDKEIFKDIFTSFIKESSATEV"
     gene            1815..3038
                     /locus_tag="PK016_07410"
     CDS             1815..3038
                     /locus_tag="PK016_07410"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HXL02923.1"
                     /translation="MKVAERVKKAYQLKDWVADRVFRLRGEGAFEVLAQAQKLEKQGK
                     EIIHLEIGEPDFDTPINVKEKAIQAIRSGSTHYVPSAGIDELREAVCVYLRRSRGIEV
                     YPEEVVITPGAKPVIFLSFLLLLEEGDEVIYPDPGFPAYRSIIEFIGAKAVPLRLREE
                     NEFRVDVDELKNLITSKTKMIVINSPHNPTGSVLTGEDIEAIARLAQEEDLLVFTDEV
                     YSEIIYDQKHVSILQFPDMKERTVMLDAFSKSFAMTGWRLGYAVLPEEWARLLSLLVT
                     NSNSCTCSFTQIAGVEALLNGEESVRAMTQEFRRRRDFMEKRIARIEGIKIVQPQGAF
                     YFFPNITSFGLSSKELADYLLKEGGVAVLPGTSFGHYGEGYLRISFANSLENLARAMD
                     KMEEALGKLTKRGGK"
     gene            complement(3120..4064)
                     /locus_tag="PK016_07415"
     CDS             complement(3120..4064)
                     /locus_tag="PK016_07415"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXL02924.1"
                     /translation="MREERSKLLIIKSDVSLLLANLIWGATFVTMKNLLESVPPLTIL
                     FWRFAIATVLLLLISLLSKWHKIDWVGGIFLGISLFAGYLFQTWGLIYTTPARSAFIT
                     GLSVILVPPLAFVILKSSIDRFSIIGTVISLFGLGLLTWQRGETKSIFFLGDFLTLLG
                     AIAYALQIVLVEKFTKDNESLPLATIEMGTVALLSLMFGIFFHQNIYPSFSRKEWGSI
                     LFLGIFATALAFTLQKRAQKHTSSIHAGLIFASEPVFAAIFSYFLWQEKFTPRTLGGC
                     ALILGGILFSQLGLLFNQPILYPRSSNQTPGLPRKEDR"
     gene            4405..5943
                     /locus_tag="PK016_07420"
     CDS             4405..5943
                     /locus_tag="PK016_07420"
                     /inference="COORDINATES: protein motif:HMM:NF020698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HXL02925.1"
                     /translation="MNKNRFLRRRGKLFILSLLVFLLWGSTLVGAQPQISIRFQLHPD
                     VYVSIPRSPQITLSIDKGEGANYYIDDPITIRYRANRDGYVNLIDYLPNGDVSILVRD
                     QLIRSGKEEKFVGTVYGPAGVERIVILLTPDKVSNADLESFIQSPHQGERIFGSRFST
                     NRVHFQVLARMESAVLTIEPDDLTLEPGTSVILTATLKDGRGRPVQGKEIRWSVNGGK
                     LSTSRTTTDLQGRSRVTFYAPSQPGTVTVEARFPGTVSLASVSTRITIRVEKAAYSAS
                     LEVTPSSFTLNGGENIKLVATLKDENGRPVSGRTIYWEVSEGDLDKYSIPTDSSGKAV
                     VYYYAPDVDETTDVKITAQFRGTSSLAPASFDVWGTIEPKAIALTSSLFYLDFGSGTP
                     RGNVDRLRYSGEIVFGFSLNDLHVLEMKPREAVEFEFNPGGFLQEGAIFLWLQGDSGG
                     KARVSVNGNRLKTISVTRDVITPDGEEKIVIPQNILVRGTNRLRIEAEPGRNIRLQKV
                     IIAF"
BASE COUNT         1856 a         1057 c         1403 g         1744 t
ORIGIN      
        1 taatgattaa cgagcttttt ttgcagggaa tagaggctcc ttgggaagaa gtggactttc
       61 gggtgggact tcttaaaatg agctggggag ctagtgacat tatgagcccg gttgatgtca
      121 taaaccccac tcctttttct ttgagtttca gcgaaaacgc tctggaaaat aaaatccctg
      181 ttcctgccct ggatttggaa tggtatcatt ctcaagattg gtcctgggaa tttttctatc
      241 agcctgattt tgtaccaaat tttattcctg aagggataga aggacagctt ttagcctatg
      301 agctggcttc tttactgtca gttaatcctc agaaattagt tttgaattta actgaagaag
      361 aaccgttagt tgatttttct caccctatat ggggaataag agcccgagga atagtaagca
      421 atgtagatgt agcggtgagt tatttgcaaa gttattatct ttctccttat ccccgggaaa
      481 ccaatgtcct tatgggactc gatggtaata gcatagtgga tgtcaccctg ggttatccta
      541 aaagaagtat gctggggctg gaattccagg gggaatttcc tcttctggaa ggctctactt
      601 ttcgaggaga tatcgccatt tttatgcctg agcgatgggt gaatcacata aatattactc
      661 agccctccgg tgaggtggtt tcttatcctc aagaaatttt tcaaaatcct tactggaaag
      721 gaagtctggg cattgattac acgacagata ataatacctt gctaaattta gtgtggatgt
      781 ggggtaatcc ttacgaagaa gggaaagatg tttctcccta ccttttcctg actgcagaac
      841 gacccagtga agataataag tggactactt tacttacctc gggtgtaagc cttcaagatg
      901 gaagcatggt aaatgtaata ggacttacct ataaaccaaa ggataactgg aatattgctc
      961 tttcttattc tttttctagc ggagccccag gaagcaagtt aggagaggtg ggagatggag
     1021 tgtttttgaa tgtgaaatac agcttctaag acagagctag aaaatcagat gatacaatgg
     1081 aatttaatcc cctgaggagg ttatggggtt ctggtggata aaaaagaaaa aatcttagaa
     1141 gcagcaaaga aggtttttgc tgaaaagagc ttttttgaag ctactttagg agacatttct
     1201 cttctttccg gaattaaaaa atcaactatt tattattatt ttggtagtaa attagaatta
     1261 ctactggaaa tcgtcagtgt gatccttcaa catgccacag agagtatcgc taacattccc
     1321 tcttctctga gttctcgaga aactttagag agacttatag agggatactt tattttcttt
     1381 tatcaggaaa gagattctat tttggttttc cgccgggtgg gctatgattt ttttgcccac
     1441 cctgaagtgt ttcgagaact tcgtcaatct tttcaaaagt ttcgaaaagt gagggaaaac
     1501 ctgggaagga gaattggtgt tttagagaca aaaaggggga ataagataga cggtacagaa
     1561 attattcgta ttattttatc cagcatcaac gcttattgga tagaagagat agttgaagga
     1621 agagaaataa aattagaaga taaagaaatt tttaaagata tttttacttc ttttataaag
     1681 gaatcgtcag ctaccgaagt ttgaagattt attttatagt cagctgtgct aaaaacttga
     1741 agaatttttg ggtagggtat tgccaaatta acttattctc gctaaagtat tgctcaataa
     1801 ggtaggtgaa aacaatgaag gtagcagagc gagttaagaa agcttatcaa cttaaagact
     1861 gggtggcgga ccgggttttt cgattaagag gggaaggagc ttttgaggtc ctggctcagg
     1921 cacagaagtt agaaaaacaa ggtaaggaga taatacatct ggagatagga gaaccagatt
     1981 ttgatacacc tataaatgtc aaggagaagg ctatacaggc tattcgaagc gggtctaccc
     2041 attatgttcc ttctgcaggt attgatgagc taagagaagc agtatgtgtt tatctgcgaa
     2101 gaagtcgagg cattgaagtt tatcctgaag aagtagtgat aaccccgggg gctaaaccgg
     2161 ttattttcct gagtttcctc cttttactgg aagaggggga tgaggtaatt tatcccgacc
     2221 ctggtttccc tgcttatcgt tctattattg agtttatagg tgccaaggca gtgccgctgc
     2281 gtcttagaga agagaatgaa tttagagtag acgtagatga gctaaagaat cttattacca
     2341 gtaaaactaa aatgatagta attaactctc cccataatcc cacgggttca gttttgaccg
     2401 gggaggatat agaggccatt gcccggttgg ctcaggaaga agatttattg gtatttaccg
     2461 atgaggtata cagtgaaatt atttatgacc agaaacatgt aagtattttg cagtttccag
     2521 acatgaaaga aagaacggta atgctggatg ctttttctaa aagttttgcc atgactggct
     2581 ggagattggg ttatgctgtt ttacctgaag aatgggcaag gcttctttct ctcctggtta
     2641 ccaattctaa ttcctgtacc tgtagtttta ctcaaatagc gggggtagaa gctcttttga
     2701 atggagaaga atcggtgcgg gctatgaccc aagaattccg tcggcggaga gactttatgg
     2761 agaagagaat agcccggatt gagggaataa agatagttca acctcaagga gccttttatt
     2821 tctttcctaa tattacttcc tttggcttga gttctaagga actggctgat tatcttttaa
     2881 aagaaggtgg ggtggcagtt ttacctggta cttcttttgg tcattatgga gaaggttatt
     2941 tgaggatatc atttgctaac tctttagaga atctggctcg ggctatggat aagatggaag
     3001 aggcacttgg taagcttacg aaaagaggag gaaaataatg atgagaatta ttctatctaa
     3061 tttcaggcga aaaggagacc ctttgagccg agcagaagag caggttattt tgcaaaaaat
     3121 catcgatctt ctttacgtgg aaggcctgga gtttgatttg aagatcgagg atatagaatc
     3181 ggctgattaa ataataagcc taattgggaa aatagaatcc ctcctaatat aagagcacac
     3241 ccccctagtg tccgaggtgt gaatttttct tgccagagaa aataagaaaa aatagcagca
     3301 aaaactggtt cagaagcaaa tattagcccg gcatggatag aagaagtatg tttttgggct
     3361 cttttttgca aggtaaaagc taaagcggtg gcaaatatcc ccaaaaagag gatacttccc
     3421 cattcttttc tggaaaaaga ggggtatata ttctggtgga agaagattcc aaacattaaa
     3481 cttaaaagag ctactgttcc catctctatg gtagcaagag gcaggctttc attatctttg
     3541 gtaaactttt ctaccaatac tatctgtaaa gcgtaggcaa ttgctcctaa taaagtgaga
     3601 aaatcaccga ggaaaaaaat agattttgtt tctcctcttt gccaggttaa aagtcctaaa
     3661 ccaaaaagag atatcaccgt tcctattatg gaaaaccggt caatcgagga tttaaggatt
     3721 acaaaagcta aaggcgggac aagtatcaca cttaagccag ttatgaaagc agatcgggca
     3781 ggggtagtgt atatcaaccc ccaggtttga aatagatagc cggcaaaaag agatattcct
     3841 aaaaaaatac cgccaaccca atcaatttta tgccatttag ataggaggga aatgagaaga
     3901 agtaaaacag tggcaatggc aaatctccag aaaagaatgg taagaggagg aacactctct
     3961 aagagatttt tcatagtaac aaaagtagct ccccatatca ggttagctaa aaggagagat
     4021 acatcagact taatgattag gagtttagaa cgctcttctc tcatagataa tagtatatac
     4081 cacttctcag cagggaagaa gcctcgatgg taattaatta cagaaatgga ctgtttcttt
     4141 ccctcttaga gggattcctt ttttctttct ccttcagtag tccttgcctt tttattctgt
     4201 ctttagcgct gcaggctgct ctgttttggg tcaagaaggt ttattcttaa ctatatacca
     4261 caaacctttg tacgagtaga taaaagagaa aaatagaaat gcccctgcga tggtattgcc
     4321 ttcaaagagg aacaagagaa ggagaaagga ggtatgatat aattagaaat acaaattaat
     4381 taatacaaga aggaggaaga gaaaatgaat aaaaatcgtt tcctccggag gagagggaag
     4441 ctatttatcc tgagtctgct tgttttcctc ctttggggaa gcacgctggt aggagctcaa
     4501 ccccagattt ccatccgttt ccagctccac cccgatgtgt atgtttccat tcctcgctca
     4561 ccacagatta ctttatctat agataaagga gaaggggcaa attattatat agatgacccc
     4621 attaccattc gctatcgggc aaatcgggat ggttatgtta acctcataga ttacttacct
     4681 aatggggatg taagcattct tgtccgtgac caattaattc gctcgggaaa agaagaaaaa
     4741 tttgtaggaa cagtatatgg tccggcggga gtggagagaa tagtgattct tcttacccct
     4801 gataaggtga gcaacgcaga cctcgagagt tttatacaat ctcctcatca aggagagaga
     4861 atttttggta gccgtttttc taccaatcgc gttcacttcc aggtgctggc gagaatggag
     4921 agtgctgttt taacaataga gcctgatgat ttaaccctgg aaccgggaac ttcggtaatt
     4981 ttaactgcta ccttaaagga tggaagagga agacctgtac agggaaagga aattcgctgg
     5041 tcggtaaacg gcgggaaatt gagcacttcc cgcacgacta cagaccttca gggtagaagt
     5101 agagtaactt tttatgctcc ttctcaaccc ggaacagtaa cggtagaagc tcgttttccg
     5161 ggaacggtaa gtttagcttc tgtttctacc cggattacta ttcgagtaga aaaagcggca
     5221 tattccgctt ctctcgaggt cactccttca tctttcactc ttaacggtgg agaaaatata
     5281 aaactggtag ccactttaaa ggatgagaat ggaaggccag tatctggccg aactatttac
     5341 tgggaagtaa gcgaaggaga cctggataaa tattccattc ctactgattc ttctggtaaa
     5401 gctgtagttt attactatgc tccagatgtg gatgaaacta ccgatgtcaa aataactgcc
     5461 cagtttcgag gaacaagcag tttagctcct gcctcttttg acgtatgggg tacaatagag
     5521 cctaaggcta ttgctcttac atcttctctc ttttaccttg actttggtag cggcactcct
     5581 cgaggcaatg tagacagatt gcgttactcc ggagagatcg tttttggttt ctccctgaac
     5641 gatttacatg ttttagaaat gaagccccga gaggcggtgg aatttgagtt taatcctgga
     5701 ggattcctac aggaaggagc tattttcctc tggttgcagg gagattcggg agggaaagcc
     5761 cgagtttctg tgaatgggaa tcgcttaaaa actattagtg taacccggga tgtaataact
     5821 ccagacgggg aagagaaaat tgttattcct caaaatatat tagtgcgagg aaccaaccgt
     5881 ttgcgaatag aagcagagcc tggccgaaac attcgcctgc agaaggtgat tatcgctttc
     5941 taaaaaagaa aacaactata aaagggttta cccattatca ctctctgtaa gaaattaaaa
     6001 gcaagatata aagaaacact gagggagaaa aagcctccct cagtggattc ccaattgaag
//
LOCUS       DAOSVE010000097         6021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_6117_length_6021_cov_68.838921, whole genome shotgun sequence.
ACCESSION   DAOSVE010000097 DAOSVE010000000
VERSION     DAOSVE010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 6021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6021
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6117_length_6021_cov_68.838921"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..1542
                     /locus_tag="PK016_07425"
     CDS             19..1542
                     /locus_tag="PK016_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HXL02926.1"
                     /translation="MKRNWWILPLVVALLFFLSGGWVGAQESSSEYSGEPAKYVFLFI
                     GDGMGIDHFSVTQDYLNALEEKNVGLKELSFTSFPAAGLVSTFDYGSYITDSASAITA
                     ILTGEKTYSGVLNMLPDKSTPLVSVAEMAKEAGMKVGVVTSVSLDHATPAGCYAHQAS
                     RNNFYEIAIEATQSDFDYFGGGGFLQPRGKNDDQRDIFEIFAENGYTVVRNREGFEKL
                     TPQPGKVVAINAVLDADQAMPYEIDREENDLSLADFTRKGIELLDNPEGFFMLVEGGK
                     IDWAAHANDAATVIHDVLAFSKAIEEAIKFYQQHPEETLIVVTADHETGGLTLGFAAT
                     GYSLYPEVLQNQKMSFLEFSKVVEQYRQNTPQEKANLSDFWPQIEENFGLLDIPAPEK
                     AQLEEKAKNGDAEAQAKLRLTLTDYEKEELEKALTMSIQGEAPQSYGGYDPLTVTLTQ
                     ILNQKAGLSWTTFSHTGAPVAIFAQGAGAELFDDYFDNTEIFAKLVSIMSLETVLVQ"
     gene            complement(1667..2575)
                     /locus_tag="PK016_07430"
     CDS             complement(1667..2575)
                     /locus_tag="PK016_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HXL02927.1"
                     /translation="MKVKCPECGFEDEGKFCSNCGAPLPQIEFPSRQETPQVPSEVSW
                     LSKCPVCKSGRLLPVVKKKLFGLVTSENWECENCGATFTRRGEQYELTKVWDVSNPIW
                     RNYKNQSLTEIEWKNIAYGGKPYREQREMDMEYWMTQLQEGNVPVHITGAKTPIILKK
                     NEKLQLVLPEISLLEPRAVRTSVYGGPGFRVAKGVYFRVGRTRSESHEELKHIDQGVL
                     TLTSKRFVFSGVKRNVNVDLRKIVSMEPYSDGIALRVENRQKTQYFTGVDQVKLAITV
                     DDRTYEEQLSGLILMYFIEGLIKKME"
     gene            2877..4133
                     /locus_tag="PK016_07435"
     CDS             2877..4133
                     /locus_tag="PK016_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL02928.1"
                     /translation="MKRFKGLSLLLSVLLVLALGTVAFSQEKGRIEIFSWWTAGGEAE
                     GLQALFDIYSKLYPDVEIINATVAGGAGANAKAVLKTRMLGGDPPDTFQVHAGKELID
                     TWVKTGFMEPLTELFQSEGWVDVMPKGILDIVSHEGDYWSVPVNIHRANVLWYNKKIF
                     ADNGLTAPQNFDEFFQIADKLQGQGIVPFVMGTKDGWEAGHIFETVLIAKLGAEGYSG
                     LWTGATKWSDPKVTEALETFKKMMTYINSDHAAFTWDEAYEYIIDGKGAMSIMGDWVA
                     GYFFSKEYTDFGWAPVPETAGIFDALSDSFGLPKGCQNRDNVINFLKVLGSKEGQEAF
                     NLKKGSIPARTDINKDLFGDYQKSVMEDWEKDVIVPSVMHGAAAAEGWVTEYKDVIAL
                     FVARPDVEVTQKALVQVAERAGIPQQ"
     gene            4199..5092
                     /locus_tag="PK016_07440"
     CDS             4199..5092
                     /locus_tag="PK016_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXL02929.1"
                     /translation="MKKTREEKILTFLFILPSVILIAVFVYGFIAWTGWVSLTNWKDV
                     IPDFTFVGFKNYIDLFNNLRFQISFKNILVFTALFLVASIAIGLLLAILLDRKVRFEN
                     IFRSIYLFPMAISFIVTGVVWRWMLNPGTTITGSLGINMLLDKIGLGFLKSGWYTDPN
                     VGIKAVVIPAVWQMSGYVMAMYLAGIRGIPQELSEAAQVDGASSWQVYRYIILPLLQP
                     ITLGAVIILTHISLKIFDLVVAMTGAGAGFSCDVPALFMYDTTFRGNFFAQGAAIATI
                     LLIFVAILIIPYLRHGLKEGS"
     gene            5229..5948
                     /locus_tag="PK016_07445"
     CDS             5229..5948
                     /locus_tag="PK016_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXL02930.1"
                     /translation="MWKLPQNISLDSFKIAWSGNASKGITGLSKNFMNSIYLVVPATI
                     ISSLLGSMNGYVFSKWKFKFSNTLLALIIFGMFIPYQSVLIPIVVVLQKIGLYGSIPG
                     LIFIHCVYGIPITTLIFRNYYSTVPTELLEAAKIDGAGFLRIYRDIILPVSMPGFAVV
                     MIWQFTSIWNDFLFGLIIAQKPSVQPITVALNNLAGSYFVEWNIQMAGALITAIPPLL
                     LYIFLGRYFMRGLLAGSLSGT"
BASE COUNT         1733 a         1197 c         1242 g         1849 t
ORIGIN      
        1 aagactggag gtagtagaat gaaaagaaat tggtggattt tgcctttagt tgtggcgctt
       61 ctctttttcc tctcgggagg atgggtcgga gctcaagagt cctcaagtga atacagcggc
      121 gagccggcca aatatgtttt tctctttatc ggtgatggaa tgggcataga ccacttcagt
      181 gtcactcagg attatctcaa tgctctcgag gagaaaaacg tcggtttaaa agagctttct
      241 tttacttctt ttccagcagc ggggttagtc tctacttttg attacggttc ctacataact
      301 gattcagcct cagctataac agctattctt actggagaga aaacctatag tggagttctt
      361 aatatgttac ctgataaatc tacacctctt gtaagtgtag ccgaaatggc caaagaagcg
      421 gggatgaaag tgggggtggt aaccagtgtt tctttagatc acgccactcc tgctggttgc
      481 tatgctcatc aagcttctcg caacaatttt tatgaaattg ctatagaggc aactcagagt
      541 gactttgatt acttcggggg aggagggttt ttacaacccc ggggtaaaaa tgacgaccag
      601 cgagatattt tcgaaatctt tgctgaaaat ggctacactg tagtaagaaa tcgagaaggt
      661 ttcgaaaaat taactcctca accgggaaaa gtggtagcaa tcaacgccgt tttggatgca
      721 gaccaggcaa tgccttacga aattgaccgg gaggaaaatg acctttcttt ggctgatttt
      781 actcgcaaag gtatcgagct tctcgataac cccgaaggtt tctttatgtt agtggaagga
      841 ggaaaaatcg actgggcggc acacgctaat gatgctgcca ccgttataca tgacgtttta
      901 gcctttagta aagcgataga agaagcaatt aaattttatc agcaacatcc cgaagaaact
      961 ctaattgtag ttactgctga ccatgaaact ggagggttaa ctctggggtt tgccgcaacg
     1021 ggatatagtt tatacccaga agtcttacag aaccagaaga tgtctttctt agaattcagc
     1081 aaagtggtag aacaataccg acagaatact cctcaagaga aagcaaatct ttcagatttc
     1141 tggcctcaaa tagaagaaaa ctttggttta ctggatattc ctgctccaga aaaagctcaa
     1201 ctggaagaga aagctaaaaa tggagatgca gaagcacagg ccaagttgag acttacttta
     1261 actgactatg aaaaagaaga attagaaaaa gctttgacaa tgagtataca aggagaagca
     1321 ccccaaagct atggtggcta tgaccccctt actgttaccc taactcaaat tttaaaccaa
     1381 aaagctggtc tgagctggac aactttctct cataccggcg ccccagtagc catcttcgct
     1441 caaggggcag gagctgagct atttgacgac tactttgata atactgaaat atttgctaaa
     1501 ttagtatcaa taatgagttt agaaacagta ttagtacaat aaaaattctg tctccgctac
     1561 tccaagggcg gggttcatga accccgccct ttttcagaac catcagaaga taaaaattaa
     1621 tttgattgca aatggttttt tcttatcccg gtaaaactta ccaaagctac tccatctttt
     1681 ttatcaagcc ctcaataaaa tacatcaata tcaaacccga aagctgttcc tcatatgttc
     1741 tatcgtctac ggtgatagca agcttaacct ggtcaacccc tgtgaaatac tgtgtttttt
     1801 gtctattttc tactcttaga gctattccat cactataagg ttccatcgac acaatctttc
     1861 gcaggtcaac attaacatta cgtttaacac ccgaaaaaac aaatcttttg ctcgttaaag
     1921 tgagaactcc ctggtctata tgttttagct cttcgtgaga ttcggacctg gtccttccca
     1981 ctctaaaata aactccttta gccactctaa atcctggccc cccatagaca ctggttctga
     2041 ctgcccgagg ttctaataaa gatatttctg gtaaaacaag ttgaagtttt tcattctttt
     2101 tcaaaataat cggtgttttt gctcctgtaa tatgtactgg aacatttcct tcttggagct
     2161 gagtcatcca gtactccata tccatttctc tctgttctct atatggcttg cctccgtacg
     2221 ctatattttt ccattctatt tctgtcagac tctgattttt ataattccgc cagatggggt
     2281 tagaaacatc ccaaacttta gtaagttcat attgttctcc ccttcgagta aaggttgcgc
     2341 cacaattttc acactcccaa ttttcactgg taaccagacc aaagagcttc tttttaacaa
     2401 ctggtaatag tcgacccgat ttgcacacgg gacacttact gagccacgaa acttctgaag
     2461 gaacctgggg agtttcttgc ctagaaggaa actctatctg aggaagtggc gctccacaat
     2521 tgctacaaaa cttgccttca tcctcaaatc cacattctgg acattttact ttcattttcc
     2581 ctcccctttt taataatact gttcttattt tatactttga taaaacatgt gtctatcccc
     2641 taccctgcag ttttgtcagg caggagttcc cttttacaga tttaataacc cccttccttt
     2701 ttgcaaacct catttttcta ttttataaaa gaccaaaagc cagattgcca tgggaagaaa
     2761 tagcaacttt aacagtaagg gaggaaacga gacagagagc cctcggtaac ttgacctaat
     2821 ttgtgggaat ggtataattt tatacagaag taaataatat taaggggggt acaaaaatga
     2881 agcgttttaa aggtttatct ttgttgcttt ctgtgttgtt agttctcgct ttaggaaccg
     2941 ttgctttctc ccaggaaaaa ggtaggatag aaatcttttc ctggtggaca gcaggtggag
     3001 aagctgaagg attgcaggca ctctttgata tttatagtaa actttatccc gatgtagaaa
     3061 tcatcaacgc taccgtagct ggaggagcag gagccaatgc taaagcagta ttaaaaacca
     3121 gaatgctggg tggagaccca cctgatactt tccaggtaca tgctggaaaa gagctaatcg
     3181 atacctgggt taaaaccggg tttatggagc ctctgactga gcttttccag tctgaaggat
     3241 gggtagatgt catgcccaaa ggtatattag atattgtttc ccatgaaggt gattactggt
     3301 ctgtaccggt aaatattcac cgagctaatg ttctctggta caataaaaag atttttgccg
     3361 ataacggatt gacagctcct cagaactttg atgaattttt ccagatagcg gacaagctac
     3421 aaggacaagg gattgttccc tttgttatgg gtacaaagga tggatgggaa gctggccata
     3481 tatttgaaac agtgttgata gcgaaattag gagcagaagg ttacagtggt ttgtggactg
     3541 gcgcaacaaa gtggtcagat cccaaagtta ccgaagcctt agagacattc aagaaaatga
     3601 tgacttacat taattctgat cacgctgcct ttacctggga tgaagcctat gagtatatca
     3661 tagacgggaa aggcgcaatg agtataatgg gtgactgggt agcaggatat ttcttctcta
     3721 aagaatacac tgattttgga tgggcaccag ttcctgaaac tgccggaata tttgatgctc
     3781 tatctgatag ctttggtctt cccaaaggat gccagaatag agacaatgtt ataaacttct
     3841 tgaaagtctt aggttctaaa gaagggcaag aagcttttaa cctcaagaag ggttctattc
     3901 ctgcccgaac cgatataaat aaagacctct ttggtgatta ccagaaatca gttatggaag
     3961 attgggagaa agacgtcata gtccccagtg taatgcacgg tgcagcagct gcggagggat
     4021 gggtgactga gtacaaagac gttatcgctc tttttgtagc tcgtccggac gtagaagtaa
     4081 ctcagaaagc tctggtgcag gtagcagaaa gagcaggaat accacaacaa taagatttcc
     4141 ctgaagagga gggaaaattc cctcctcttc tttttttctc ttttagaggg agagatttat
     4201 gaaaaaaaca agggaagaaa aaatcttaac tttcttgttt attttgccct ctgttatcct
     4261 tattgctgtt tttgtctacg ggtttattgc ctggacagga tgggtgtctt taaccaactg
     4321 gaaagatgtg attcccgatt ttaccttcgt gggatttaaa aactatattg acctctttaa
     4381 taaccttcgt ttccagataa gctttaaaaa tattttagtc ttcactgctt tgtttcttgt
     4441 ggcatccatc gctattggtc ttcttctggc tattctttta gatagaaaag ttcggtttga
     4501 gaatatcttc agaagtattt atttatttcc tatggccata tctttcattg tgactggtgt
     4561 agtatggagg tggatgctaa accccggcac gaccatcact ggttctttag gcattaatat
     4621 gctattagac aaaataggtt taggattttt gaaaagtgga tggtatactg accctaatgt
     4681 aggcataaaa gcggtggtaa ttcctgcagt atggcagatg tcaggatacg tcatggctat
     4741 gtatctggca ggcatcagag gtatacctca agaattatct gaagcagccc aggtagatgg
     4801 agccagtagc tggcaggtgt acaggtatat tattttacca ttattgcaac ctattacttt
     4861 aggagcagtt atcatcctta ctcatatctc cttaaaaata tttgacttag tggtagccat
     4921 gaccggagca ggagctgggt tttcctgtga tgtcccggct ctatttatgt atgataccac
     4981 gtttagaggt aacttctttg cccaaggcgc agccatcgcc actattttgt tgatctttgt
     5041 agctattctt atcataccct atttgagaca cggcctgaag gagggaagct aaaatacgca
     5101 gggcttataa atcaagtaga tttattttat acctcgttct ggtattattc gccatatttt
     5161 accttttacc tgtatatgta cttctagcaa ccagcttcaa aagctttgcc gaagtgggtt
     5221 taaaagacat gtggaagctc cctcaaaata taagtctgga cagttttaag attgcctgga
     5281 gtggtaatgc ctcaaaagga ataaccggtc tatctaaaaa ctttatgaat agcatatatc
     5341 tggtagttcc tgccaccata atatcttctc ttctgggttc tatgaacggt tatgtgttct
     5401 ccaagtggaa attcaaattt tctaatacct tgctggctct cattatattt ggtatgttta
     5461 ttccttatca gagcgtttta attcccatag tggtagtttt acagaagata gggttgtacg
     5521 gctctatccc gggcttaatc ttcattcact gtgtttatgg aatacccata actacattaa
     5581 tattcagaaa ctactactct accgttccta ctgagctctt agaagcagca aaaattgatg
     5641 gagctggatt tttaagaatc tatcgagata taatcctacc agtatctatg cctggttttg
     5701 ctgtagtgat gatatggcaa tttacttcta tatggaacga ctttctcttt ggactgataa
     5761 tagcacaaaa gcccagtgtt caacctatta ccgttgctct caataacctc gctggctcat
     5821 attttgtaga atggaatatc cagatggcag gagctctcat taccgccatt cctcctcttt
     5881 tgttatacat tttcctgggt cgctacttta tgagaggact tttagcaggc tctttatctg
     5941 gaacatagct ccacataaat tattgcatta tagtatcccg caaaactggt taaaaacttt
     6001 agaaagtaaa ttatatcgaa a
//
LOCUS       DAOSVE010000098         5618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_6541_length_5618_cov_81.218407, whole genome shotgun sequence.
ACCESSION   DAOSVE010000098 DAOSVE010000000
VERSION     DAOSVE010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6541_length_5618_cov_81.218407"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="PK016_07450"
     CDS             complement(<1..1102)
                     /locus_tag="PK016_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HXL02931.1"
                     /translation="MRKVKRGRFLNNLVVTLVFLLFLSLVDNACAEVNVGKQLVNPKD
                     FVVEETTVYPAIPGHSLPLAISIVTNFEDFSGKIPLSSTAIELLQKNAFVVIPTPLNI
                     GEQEIYLPPSWEATNPKDDFVAYYKVLKNIDVPIFITSDSLLHYYHIFFDTTLMRMER
                     DLFYQDIWAISRELFEASQKDYEQSEGDLKEAARRNMAYLSVALELLKPEKDQVINDE
                     ILKEEYCSPEMDPEYCELMIAGVKDVYGEKASYQYFSEAEFERYHFETPEIVKELVQS
                     EIELIEAHEGWEYSPIFIYQEDYSQYVPRGHYTKSERLKNYFKALMWYGRMTALIEGS
                     PSLSPGESLCGGRIEGIISDYDARIQTLQAFLL"
     gene            complement(1127..2839)
                     /gene="amrB"
                     /locus_tag="PK016_07455"
     CDS             complement(1127..2839)
                     /gene="amrB"
                     /locus_tag="PK016_07455"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HXL02932.1"
                     /translation="MRNFPQLLLLLLVCSLITVTAFGNSGEALSVVSGVVPHHLLAKE
                     IMVGFFSFIAREEQLPDTIILLSPDHFHSAALERGNSFISVNWESDDVELGDVAVDRE
                     LLTKIATKIRIRPNQGAVLSEFGLMNLLPLIKEYLPEVKIVPILIPADISREEVAQLV
                     NTINQVASLQTIMVASVDFSHYLPSRAASFHDVKSIRVLLNGEEEHFENIEVDSWQSL
                     YAVRLFARLRKKEKPVVIAHKNSVDFLSLSPNETTSYFSVVFEEGEAENEVEAETILF
                     AGDMMLGRGIAELNKKNGIYYPFQKIGRLLRGVDVVFANLEGPVGENPLEFGGDKLRL
                     VFLPRVLEGVAWSKINLVSLANNHIMDRGAEGVQETRDWLDKYQIRHIGNPLYSYQDE
                     LNPCFSTEHSVFLAFNRVLPFTHREEEIVREIRLAKIDNPDKFIIVSIHWGEEYQLKS
                     SSSQRQLARRMIAAGADIVVGHHPHVVQEIELVQGKPVFYSLGNFIFDQQSIPETQEG
                     LIVGLAVEPDKATCYLFPIQHHLGQPMLMSRGRVDEFLVGVAEKSDKKLIEDIKRGII
                     EIKR"
     gene            complement(3180..4538)
                     /gene="mgtE"
                     /locus_tag="PK016_07460"
     CDS             complement(3180..4538)
                     /gene="mgtE"
                     /locus_tag="PK016_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HXL02933.1"
                     /translation="MEERNIISFKESVEQMLISQDYEALQELLEELHPYEVATLLEEL
                     EEPEQLKILELMPADMAAETLEHLDYEDQYRLLDHLEDTVARKIIATMPSDAIADLVM
                     AIHPHQAQKILDMVPEEYLQRIEYLMTYPEDSAGGIMSLEYVAVRQHWTVERVIEHFR
                     KVGRRGSAVNYIYVVDNIGRLVGVVSLKELLLSHPKTPVSEIMHTNVVAIPVDADQEE
                     AAKLMSQYDLLVLPVINGRGRLVGVITADDIMDVIEEENTEDIHKLGGSNPLDLPYMQ
                     SSLTTLFSKRINWLIFLFAVQAITSNILKHFQSALSSVVALSFFIPLFTDTGGNSGTQ
                     ASSLIIRSLALGEVTTEDTTRILLKELQVSAMLGAVMGVVGYFFAWVIAGSVAIAIAV
                     AITLFVVLVVSSTIGAILPIVGSKLGIDPAVFSAPLITTIVDIVALMLYFNIATLILG
                     IR"
     gene            complement(5002..5172)
                     /locus_tag="PK016_07465"
                     /pseudo
     CDS             complement(5002..5172)
                     /locus_tag="PK016_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869125.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
BASE COUNT         1730 a         1309 c         1003 g         1576 t
ORIGIN      
        1 ctaacaaaaa cgcttgcagg gtctgaatcc gggcatcata gtcagaaata attccctcta
       61 tccgaccacc gcagagagat tctcctggcg ataaagaagg agagccttct atcaaggccg
      121 tcattctgcc ataccacatg agcgctttaa aataattttt caatctttct gatttagtat
      181 aatgcccccg gggaacgtac tgagaataat cttcttgata gataaagatg ggtgaatatt
      241 cccaaccctc gtgtgcttct attaactcta tctctgactg caccaactcc ttaactattt
      301 cgggagtttc aaagtggtaa cgctcaaatt ctgcctcact aaaatattga tagctggctt
      361 tctctccgta aacatctttc acaccagcta tcattaactc acaatattcc gggtccatct
      421 ctggagaaca gtattcttct tttaagattt catcgttgat tacctggtct ttctcaggct
      481 ttaaaagctc cagggctaca gaaagataag ccatatttct tcgggctgct tcttttaaat
      541 cgccctccga ttgctcataa tctttttgtg atgcttcaaa caattctcgg ctgatagccc
      601 agatatcctg atagaaaagg tctctctcca tccgcatcag ggtggtatca aaaaagatat
      661 ggtaataatg taaaagagag tcagaggtaa tgaaaattgg tacatcaata tttttcaaca
      721 ctttgtaata agcaacgaaa tcatctttgg gatttgttgc ctcccaggaa ggaggaagat
      781 aaatttcctg ttctcctata ttaagggggg tgggaatgac cacaaaggca tttttctgca
      841 acaactctat tgcagtagaa cttaagggaa tcttccccga aaaatcttca aaattagtaa
      901 caatagagat agctaaaggc aaggaatggc cgggaatagc aggataaact gtggtttcct
      961 ctaccacaaa atctttcgga ttaaccagtt gcttaccgac attaacttcg gcacaggcat
     1021 tatctaccaa ggaaagaaac agcaaaaaca ctaaggtaac tactaaatta tttaaaaaac
     1081 ggcctctttt tacttttctc atttttaccc ctccttattt ttctatctat ctttttatct
     1141 ctattatacc tctctttata tcctctatta actttttatc agacttctca gccacaccta
     1201 cgagaaattc gtctacccta cctctactca tcagcatcgg ctgacctaaa tggtgctgga
     1261 tagggaaaag ataacaggta gctttatcag gttcaacggc caagcccact atcagtcctt
     1321 cctgagtttc aggtatactt tgctggtcaa agataaagtt acccagggaa taaaagactg
     1381 gcttaccctg taccaattca atttcctgta ccacatgggg atgatgtccc acaacgatat
     1441 ccgctcccgc agcaatcatg cggcgagcca attgccgctg ggatgacgag ctttttaact
     1501 gatattcttc tccccagtgg atgcttacaa ttataaattt atccgggttg tctattttgg
     1561 ctaaccttat ttctctaaca atttcctcct ctctatgggt aaacggtagc actctattaa
     1621 aagccagaaa aacggagtgt tcagtagaaa aacagggatt tagctcatcc tgatagctat
     1681 ataaaggatt accaatatgg cgaatctgat atttatccag ccaatctcta gtctcttgca
     1741 cgccttctgc tcccctatcc ataatatgat tgttggctaa ggacacaaga ttaatcttac
     1801 tccaggcaac cccttcaaga acccggggaa gaaagacaag tcgcagttta tcgcccccaa
     1861 attccagcgg attttcccca acaggacctt ctaagttggc aaaaacaaca tcaacgcctc
     1921 tcagtaatcg cccaattttt tgaaaaggat aataaatacc attcttttta ttcagctctg
     1981 ctatccctcg ccccagcatc atatccccag cgaataaaat tgtttctgcc tcgacctcat
     2041 tttcagcttc tccctcctca aaaaccacgc tgaaatagga agtagtttca tttggagaaa
     2101 gggataagaa atccacagaa tttttgtgag caatcacgac cggtttttct tttttccgca
     2161 accgggcaaa taacctcaca gcatacagtg attgccagct atccacctct atattttcaa
     2221 aatgctcttc ctcaccgttc aataaaaccc taatgctttt aacgtcatgg aatgatgctg
     2281 cgcgagaggg gaggtaatgg gaaaaatcga cactggctac catgatagtt tgaagagaag
     2341 caacctggtt aattgtattt accagctgag ccacctcttc ccgggaaatg tcggctggta
     2401 tcaaaatagg cacaattttc acttccggaa gatactcttt gattaaaggc aagaggttca
     2461 taaggccaaa ttccgaaagc accgcaccct gatttggcct tatcctgatt ttagtagcta
     2521 tctttgttaa caattccctg tcaacagcga catcacccag ctcaacatcg tccgattccc
     2581 aattaacact gataaaggaa ttgcccctct cgagggccgc actatgaaag tgatctgggc
     2641 ttaatagaat gatggtgtca ggaagctgct cttctctggc aataaaactg aaaaaaccca
     2701 ccataatttc tttagctagc aagtgatgag gcacaacacc ggaaaccaca gacaatgcct
     2761 cgccagaatt accgaaagca gtcaccgtaa ttaaagagca aaccagcaaa agcaataata
     2821 gctgagggaa atttctcata tctatgatat tttacaacat ataaataacc tacggcataa
     2881 gactaaggaa aagattaaaa atgcaaaaag taaaggtact aaagaagaaa aggcatacct
     2941 tcctcagtgt ctgcaacgac aatatttcaa aagaaacact accatgaatt ttgcagttaa
     3001 ctatacataa ataaaaatgc ggataaacac atcaaatgac tttaacccag aaaccagacg
     3061 ctcagaagcc accataatct tctggcgaaa aaccattatt tacagcgata tggaaaagaa
     3121 tataggtcat caagatatct tggaactcct gaatttataa tcacactacg ataatcaaac
     3181 tatcttattc ccaaaattag ggtagcaatg ttaaagtaaa gcattaacgc tacaatatcc
     3241 actatcgtag taatgagagg ggcagaaaat actgccggat ctatacccag cttgctacct
     3301 actatcggta gtatcgctcc tatggttgaa cttacaacta aaaccacaaa aagggtgatg
     3361 gctaccgcaa tagcaattgc taccgaaccg gcaataaccc aggcaaaaaa gtatcccact
     3421 acacccatca cagcacccag catagcacta acctgaagct ccttaagtaa aatcctcgtg
     3481 gtatcttcag ttgtgacttc tcccagagcc agagacctga taatcagaga agaagcctga
     3541 gttcctgaat taccacctgt atcagtgaac aaaggaatga aaaaagaaag agccacaaca
     3601 ctacttaaag cactttgaaa atgcttgaga atattgctgg ttatcgcctg aacagcaaag
     3661 agaaaaatca gccagttaat tcgcttacta aaaagggtag tcagactcga ctgcatgtaa
     3721 ggcaaatcca gaggattact tccccctaat ttgtgaatat cttcagtgtt ttcttcttca
     3781 atgacatcca ttatgtcatc tgcggtaata actcctacca ggcgtcccct accatttata
     3841 actggaagaa ctagcaaatc gtactgactc atcagctttg ccgcttcctc ctggtcagca
     3901 tcaactggta tggctaccac attggtatgc attatttctg aaacgggtgt tttaggatga
     3961 ctcaacagca attctttgag agatacaact cccaccaacc gcccaatgtt gtcaactaca
     4021 taaatatagt taacagcact tcccctacga cctactttgc gaaaatgctc aatcactctt
     4081 tcaacagtcc agtgctgtct tactgcaacg tattccaggg acattatacc ccctgcagaa
     4141 tcttcggggt aagtcataag atattcaatc ctttgtagat attcttcagg aaccatatcc
     4201 agaattttct gagcctggtg gggatgaata gccataacga ggtctgctat ggcatcactg
     4261 ggcattgtag caataatttt tctggctaca gtatcttcta aatggtcaag aagccggtat
     4321 tggtcttcat aatccagatg ctccagggtt tccgctgcca tatctgcagg catgagttcc
     4381 agaattttca gttgctctgg ttcttccagt tcttccaaga gtgttgctac ttcataaggg
     4441 tgcagttctt ctaataattc ttgtaaagct tcatagtctt gagaaataag catttgttca
     4501 acagattctt tgaaagatat aatgtttcgc tcttccacag atatcacctc caatagcaaa
     4561 tttttaaagg ttcaaaataa gtttgctgaa aatgtgatgt tcgattctta ctctccaacg
     4621 atacctgaaa ttctttaagg tttttgcggg gccagcagac agaaaggggg gaagaacgtc
     4681 agctacctgt tagccaggat acacctgata cattcaaaat gcactgggaa aggtggtagc
     4741 cctggcgcaa aaccccaaag aagccagcac attaagggaa atgtagctca tgaccgaact
     4801 ttcaatattc aattgtttct ggcacctatg actaccaggt tcagcgtcca tttcatacca
     4861 ccaccttcag taacacaaag ataaccgttt ataagtttat catatctcca aaaaatttgc
     4921 agctatctca aaaataagac caactcaatt gtggttaacc gcataattct gtacctctca
     4981 actcaattgc gcattgagcc cctgagcttc ccctcgggga tgttctaaag gaagatatcc
     5041 ctgttcatct ttatacattt ctttctttac cttcctgaca taattggcta ctgtcctgta
     5101 gcttaccttc agtagatcag catgttcttc ttttaatctg gtgaatatct tccgagcggt
     5161 atgtctttgt ttgaaagtca agtataaact actagaagta atcagtgtag tgacaaaata
     5221 gcagattcta aattaaagaa tatagatttg atgcgggttt caactaagta aagtgtcaaa
     5281 atcaggataa agagaagcac ccctttataa tcctccaaaa cacaaaagac aaaaactact
     5341 gaggaaggag aaaccttcct cagtataagg gggaaagaat tcccccctta tgaaccctct
     5401 ttctttttct tccctcgcct tcaatggaag aaccccacat attccttccc tcgcccttga
     5461 agggagagct ttacatcttt ttcccctctc cctcgacgga acctacatct tcttttccct
     5521 cgccctcgaa gggagagggt tagggtgagg gtgtcttctt gccgtcattc ctgagtgtat
     5581 ttatcaggaa tccagaacta taagggggta aaccccca
//
LOCUS       DAOSVE010000099         5610 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_6557_length_5610_cov_61.036724, whole genome shotgun sequence.
ACCESSION   DAOSVE010000099 DAOSVE010000000
VERSION     DAOSVE010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5610
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6557_length_5610_cov_61.036724"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="PK016_07470"
     CDS             complement(<1..740)
                     /locus_tag="PK016_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HXL02934.1"
                     /translation="MSSNDEIIKTENLTKVFGSGKKAIKAVDDVSISIKKKEVISLVG
                     QSGSGKTTLARMLLRLLKPTAGKIIFEGQDVTNIEGKEKKAYWRKVQAIFQDPYASFN
                     IFSPVKKVLLDAFKLFDNKFTKEEKWRMVCESLEAVNFRPDEVADKFPFELSGGQRQR
                     VMIARAHLIKPELLIADEPTSMIDANLRSGILELLLDLRDTEGTTIMFVTHDLGLAYY
                     VSERLLIMHEGKIVEEGEADKIISEPDHP"
     gene            complement(727..1752)
                     /locus_tag="PK016_07475"
     CDS             complement(727..1752)
                     /locus_tag="PK016_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL02935.1"
                     /translation="MEESRKIMLKVDGLRAYYRTRSGERIRAVDNISFNLMEGEILGI
                     AGESGCGKSTLAMAMSGLFLPPLTYESGSVFLDGENVIGKKEEELRKKILGRKYSYIP
                     QSAMNALNPTLKIKNFVIDLLKEHDPRMSKNKVLDLTTERFESLSLPPWVIDLYPLEL
                     SGGMKQRVVIAVSTLMNPKVVVADEPTSALDVTSQKIVIKLIKELFDEGIVKSVIFIT
                     HELPILRHVCDRIAVMYAGEFVEIGETEGIIFNPIHPYSQALMQSILVPEVGIKGKKL
                     PNLPGSPPDLRKPIKGCRFADRCYLAIEDCRKEAIGMVEFDGRKVRCIRVDHIKAKEE
                     VVPHVQQ"
     gene            complement(1755..2588)
                     /locus_tag="PK016_07480"
     CDS             complement(1755..2588)
                     /locus_tag="PK016_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL02936.1"
                     /translation="METLKLLFKSPKFIAGLGIFVFLFLTAFIYPAVVDKNPFEMVGL
                     MYEAPSSRYILGTDNFGRDVFVELIHGMKTSLIIGLFAGVIATAIGVTIGLFAGYKGG
                     TVDNILNSITNIFLVIPPFVILILITVSLRSRSLAVMGLVLGITSWPWTARAVRAQSM
                     SLRGREHVDIARLNGASVVEIIIREIMPYILSYIFMAFVLQVASGILNEAGISMLGLG
                     PSNVVSLGTMLSWALLFESVRSGAWWAFVPPAITIALITFSLYFMNTGMDEIFNPRLR
                     S"
     gene            complement(2590..3600)
                     /locus_tag="PK016_07485"
     CDS             complement(2590..3600)
                     /locus_tag="PK016_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019003674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL02937.1"
                     /translation="MRKFTRYILQKFSWYLLAFFLALFLNFLLPRLVPGDPISVLVSQ
                     MMSGAVTSEAQERVYQSFIQEFGLDKPFFVQFFTYVGNVFKGNLGTSFSLYPLSVNKI
                     MADALPWTLFLQFPAIIVGWIVGNLLGAFAAYRKGNFDRILFPVALFINSVPYYCLAI
                     ILLYVFGVYLRWFPIGGGYSRTLLPSWSWPFIVDVLHHYFLPFISIVLVTIGGQAIGM
                     REMAIYELNTDYVTYCKMLGMGDRKIQSYVFRNAMLPQITGLAISLGTMVGGSLITEI
                     VFGYPGIGSTLFNGIRRLDYPLIQGSTLIIALTVLAANFIIDIVYGLVDPRIKAAQVE
                     GS"
     gene            complement(3675..5408)
                     /locus_tag="PK016_07490"
     CDS             complement(3675..5408)
                     /locus_tag="PK016_07490"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL02938.1"
                     /translation="MKKAVLTCFLVLVLALGGVAFGQQVQIPREEALYVAGFQWGPPT
                     TDNPFTGSPMTFVSNPRQHIWIYEPLFTYDGLLTGDFVPILGESFQWRDDLTLEVKLN
                     PNAYFHDGEKVTADDVVYSYKLGQKYPIGYSSVWDWLEDVYKEDDQTVIFKMKADNPN
                     KLMVEDGLGAAYIIPEHIWAKVEEDAGNDITEIRKFDNKDPIGSGPYRVYYESPETII
                     LERVDDYWGVVLYDGELPRPKYVVHPIFKSNDEGNLAFENGEVDLSQQFMPRIWECWE
                     NKGLPIGTWYKEIPYHVPATMPSLWFNVNKPPLDNPELRRAIAYCIDYAKISELAMSN
                     YSPKVEASLIVPYGAQAKYFNKELVKQEGWEYNPEKAVEILEGELGAKKGSDGIYVLP
                     DGTRLGPFKAECPYGWTDWNAALEIVAQSAKAIGIDIETSFPDAPVAYDNRETGNFDM
                     TMWTPSEPSPAQPWLNFQIVLHSKGVPEYGKIAYRNFGRYKNERADELIDLIPTVTDE
                     AELKKLYDELDSIYRKDVPVIPLMYRPWVFYEYNETYWKGFPNDDNPYAPGMPGVGAG
                     IKILWHIEPAK"
BASE COUNT         1661 a         1324 c          952 g         1673 t
ORIGIN      
        1 ggatgatcag gttcagatat tattttatca gcctctcctt cttctacaat ttttccttca
       61 tgcataatca ataatctttc gctcacatag tacgccagac ctaaatcatg ggtaacaaac
      121 attattgtcg taccttccgt atctcttaag tccaacaata actccagtat ccctgacctc
      181 aagttagcat ctatcataga agtgggttca tcagctatca aaagctcggg ctttatgaga
      241 tgagctctgg ctatcattac tctctgtctt tgtcctccac tgagttcaaa aggaaattta
      301 tctgctactt catccggtct aaaattcact gcttctaaag attcacacac cattctccat
      361 ttctcttctt tagtaaattt attgtcaaac agtttgaaag cgtctaataa tactttttta
      421 actggcgaaa atatattaaa agaagcataa ggatcttgaa atatagcctg cactttcctc
      481 cagtaggcct ttttctcttt tccttcaata ttggtaacat cttgaccctc gaatattatt
      541 ttccctgcag tcggttttaa aagtctgaga agcatcctgg caagagtggt ctttccgctt
      601 ccactttgtc ccactaaaga aataacttcc ttctttttta tggaaatgct aacgtcgtct
      661 actgctttta tagctttttt gccactccca aaaacttttg ttaaattttc cgtttttatt
      721 atttcatcat tgctggacat gaggtaccac ttcctctttt gccttgatat ggtccaccct
      781 tatacatctc acttttctac catcaaattc caccatgcct atagcttctt ttctacaatc
      841 ttctattgcc aaataacatc tatcagcgaa tctacaccct tttattggct ttcttaaatc
      901 aggaggtgat cccggtaaat taggaagctt tttacctttt attcctacct ccggtaccaa
      961 tatagattgc attaaagcct gagaataagg atgaatagga ttaaatatta taccttcagt
     1021 ttcccctatc tccacaaact ctccagcata cattactgct attctgtcac atacgtgcct
     1081 caatataggc aattcgtgag ttataaatat cacgctttta actatccctt catcaaaaag
     1141 ctcttttatc aattttatta caatcttttg ggaggttaca tccagagcag aagtgggctc
     1201 atccgccact accactttag gattcataag cgtggacacc gctatcacta ctctctgctt
     1261 catgcctccg cttagctcca gaggatagag gtctatcacc cagggaggca gagaaagcga
     1321 ttcaaacctt tccgtagtta aatctaaaac tttatttttg ctcatacgag gatcgtgctc
     1381 ttttaaaagg tctataacaa agtttttaat ttttaaagtg ggattcagtg cattcatggc
     1441 gctctgaggt atataggaat attttctccc cagtattttt tttctcaact cttcttcttt
     1501 tttgcctatt acgttctcgc catccagaaa caccgaacca ctttcataag tcaggggagg
     1561 taaaaaaaga ccggacatag ccatagccag tgttgatttg ccgcaacccg attcacccgc
     1621 tatgcctaat atctcccctt ccatcaagtt aaacgaaata ttatcaactg ctctgatgcg
     1681 ctctcctgac cttgttctat aatatgctct gaggccatct acttttagca ttattttcct
     1741 ggattcttcc acttttagct cctcaatcgg ggattaaaaa tttcgtccat cccggtattc
     1801 ataaaataca gagagaaagt tattaaagct atggttatag ccgggggtac aaaagcccac
     1861 cacgccccag atctaactga ctcaaataaa agagcccagg ataacatagt accaagcgaa
     1921 accacattac tggggccaag ccccagcata cttatacctg cttcattaag aattccgctt
     1981 gccacctgga gaacaaaagc cataaatatg taagacaaaa tatagggcat gatctccctt
     2041 attattattt ctacaacgct cgccccattg agtcttgcta tatcgacatg ctctctaccc
     2101 cttaagctca tagactgggc tcttactgcc cgggcagtcc agggccagga agtaatcccc
     2161 aataccagcc ccatcacagc aagggaccga ctgcgaagac tcacagttat cagtatcaaa
     2221 atgacaaatg gtggtataac taaaaaaatg ttggtaatag agttcaaaat gttatctaca
     2281 gtgcctccct tatacccggc aaaaagacca atggttactc ctatagctgt agctatcacc
     2341 cctgcaaaca aacctattat cagagaggtc ttcattccgt gtatcagctc cacaaataca
     2401 tctcttccaa aattgtctgt tcccaggata tatcttgaag agggggcttc atacatgagt
     2461 cctaccatct caaaggggtt tttatcgacc actgcaggat atataaacgc agttaaaaac
     2521 aaaaaaacaa aaatacccaa tcctgctata aactttggag atttaaagag tagtttcaga
     2581 gtttccatat cagcttccct caacctgcgc agctttaatc cgagggtcta ccaggccata
     2641 gactatatct attatgaaat tcgccgccaa taccgttaaa gctattatca aagtcgagcc
     2701 ctgaatcaga ggataatcaa gtcgtcttat tccattgaat agcgttgatc ctattcctgg
     2761 atatccaaac acaatctcgg ttatcaacga ccctcccacc atggttccta aactgatagc
     2821 aagaccggtt atctggggta acattgcatt tctgaacacg taactttgaa ttttcctatc
     2881 ccccatacct aacatcttac aatatgtaac ataatcggtg tttagctcat atatggccat
     2941 ctctctcatc cctatagcct gcccaccgat tgtcaccagc actatagata tgaaaggcaa
     3001 aaaatagtgg tgcaatacgt ctactataaa tggccaggac caggaaggaa gtagagttct
     3061 actatatccc ccgcctatag ggaaccagcg caggtacaca ccaaaaacgt acaataaaat
     3121 aattgccaga cagtaatagg gcacagaatt gataaataat gcaacaggaa ataaaatcct
     3181 gtcaaaattt ccttttctat aggcagcaaa agctcccaat aagtttccta ctatccatcc
     3241 tacaattatg gctggaaatt gtaaaaaaag cgtccaggga agagcatcag ccattatctt
     3301 attgaccgag agaggataaa gactgaagga ggtccccaga ttacctttga acacatttcc
     3361 aacataggta aagaattgaa caaaaaacgg tttatccagt ccaaactctt gtatgaaaga
     3421 ctgataaact ctctcttgtg cttcactggt cacggctcca gacatcatct gggaaactaa
     3481 cactgatatg gggtctcccg gaacgagccg gggaagaaga aagtttaaaa acagagcaag
     3541 gaaaaaagcc agaagatacc aagaaaactt ctgcaaaatg tacctggtaa atttcctcat
     3601 cgggctacct cggattaagt attgtatttt taaagcccgt ggagaaataa cctccacggg
     3661 ctttaaaaat aaggttattt cgcgggctca atatgccaca gtatctttat acctgctcca
     3721 acaccaggca tacctggagc atagggatta tcatcgttgg ggaatccctt ccagtaggtc
     3781 tcattgtatt catagaatac ccagggtctg tacattaatg gtattacagg aacatcttta
     3841 cgatatatac tatccagttc atcatacaat ttcttcaatt ctgcctcatc tgtaactgtg
     3901 ggtattaaat caataagctc atctgctcgt tcatttttat atcttccaaa gttcctgtag
     3961 gcaattttgc cgtattctgg tactcccttg gagtgaagca ctatctggaa attcaaccag
     4021 ggctgagcag gactgggctc agaaggtgtc cacatggtca tgtcaaaatt accggtttct
     4081 ctgttatcgt aggcgacagg tgcatcaggg aaggaagttt ctatgtctat acctatagct
     4141 ttagctgatt gagcaactat ttctaaagca gcattccagt ctgtccatcc atacggacat
     4201 tcagctttga aggggcctaa cctcgttcca tcgggaagta catatattcc gtcacttccc
     4261 tttttagctc ctagttcccc ttccagaatt tccactgcct tttccggatt gtattcccag
     4321 ccttcttgtt tcactaattc tttattaaag tattttgcct gtgcaccata aggcactatt
     4381 agacttgctt caactttggg agaataatta gacatagcca gctcagaaat ctttgcataa
     4441 tcaatgcagt aagcaatagc tcttctgagt tcaggattat ctaagggagg tttatttacg
     4501 ttgaaccaca gagaaggcat ggttgctgga acatggtaag gaatttcttt ataccaggtt
     4561 ccaatgggca atcctttatt ttcccagcac tcccagattc gaggcataaa ctgctgagaa
     4621 agatctactt ctccattttc aaaagccaag tttccttcgt cattactctt aaaaatagga
     4681 tgaacgacgt attttggtct gggcagttcg ccatcgtaga gaaccactcc ccagtagtca
     4741 tcaactcttt ccagaattat agtttctgga ctttcataat aaacccgata aggtcctgag
     4801 cctatggggt ctttgttgtc gaatttcctg atttctgtta tatcattgcc ggcatcttct
     4861 tccacttttg cccagatatg ttcaggaatt atgtatgcag cacccaaacc atcttccacc
     4921 attaatttat tgggattatc agctttcatt ttgaaaataa cagtctggtc atcttctttg
     4981 taaacatctt ccagccaatc ccagacggaa gaatagccta ttggatattt ctgaccaagc
     5041 ttataggaat aaactacatc gtcagcggtg actttttctc catcatgaaa ataagcattg
     5101 gggttcaact ttacttccag agttaaatca tctcgccact ggaacgattc tcccagtatg
     5161 ggaacaaaat caccggttaa gagaccatca taggtaaaca agggctcgta aatccagata
     5221 tgttgccggg ggttagaaac aaaagtcatg ggactcccgg taaaaggatt atcggtggta
     5281 ggaggacccc actgaaatcc tgcaacatag agagcttctt ctcgaggaat ttgcacctgt
     5341 tgaccaaaag ccactccccc taaagccaaa accaacacca agaaacacgt taaaactgcc
     5401 tttttcatag tacttctcac cccttcttaa aatattagcc atagaaacct atggctaatt
     5461 cgaattatag cacaaataat tatcattaga aatagtacca gaaataaaca aagaaaataa
     5521 gggtcaaaag actaatttcc cctttctcac ccttgcatat cccgaagacg ctcttttcaa
     5581 ccggtccata attgctcttc ccaatccttt
//
LOCUS       DAOSVE010000100         5450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_6760_length_5450_cov_70.529564, whole genome shotgun sequence.
ACCESSION   DAOSVE010000100 DAOSVE010000000
VERSION     DAOSVE010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6760_length_5450_cov_70.529564"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..918)
                     /locus_tag="PK016_07495"
     CDS             complement(133..918)
                     /locus_tag="PK016_07495"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02939.1"
                     /translation="MRKIVRVLTDSPLSGYENMSRDEALLEIGSSNALLTLRFFEWRE
                     KTLSVGRFQKTREIDFDYLKTKNLPLVRRPTGGRAILHDKEVTFSLFIPASPFKRHTF
                     YYEELQKVLTRMIGKMGIKVDEKVESGFYTSSPSCFSIHVSHEIMVRGKKIIGVAQAR
                     GEKGNLFQASFILEGDREKIASCFKEKEKVQKELENKFLPLNEILSSLPEKKEIYRLL
                     LESLEEEWGVEIEEGEWSAEELQKAEELFCTKYYPDSLYHTER"
     gene            complement(915..1295)
                     /gene="gcvH"
                     /locus_tag="PK016_07500"
     CDS             complement(915..1295)
                     /gene="gcvH"
                     /locus_tag="PK016_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011066779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HXL02940.1"
                     /translation="MNIPDDLFYTREHFWVKKGDVVTCGITDYAQNELGDVVFVELPD
                     VGKKIKQGDRIGEVESIKTVSYLYAPLSGEVVEVNKELDKKPELINQSPYREGWICKV
                     QPENEEEFKNLMSKEEYEAFLKTQ"
     gene            complement(1301..2392)
                     /gene="gcvT"
                     /locus_tag="PK016_07505"
     CDS             complement(1301..2392)
                     /gene="gcvT"
                     /locus_tag="PK016_07505"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HXL02941.1"
                     /translation="MTLKELPLSNFHRSLGARMGVFSGWKMPLYYRSIREEYFSCINS
                     CGLFDISHLGKFFLRGKEVTPFLDYATCRQVSKVKEGKGKYTFFLNWRGGIVDGVTIF
                     REKEEEWLLISNAGAREKLFQWLDYLIKNQKWEVEVEDVTEKKTLFALQGPQAQQTVR
                     QLFPVPNDNWPKFSFRQFSPGFQIIHLAFSREDGFEIMSDVSLGLEWWKKLVEEVESQ
                     GGALCGLGARDLLRFEATFPLYGQEIDETITPLELGREDLIDWEKIDFIGKKPLLLER
                     EEGSKKKLVGLEILGRRIPRYGYPLYTKEGMPCGYVTSGNYSFSWHKVLALGFVSSAC
                     SGRGENLLVAAPRTRLEAKMVSLPFGEGG"
     gene            complement(2404..3792)
                     /gene="lpdA"
                     /locus_tag="PK016_07510"
     CDS             complement(2404..3792)
                     /gene="lpdA"
                     /locus_tag="PK016_07510"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HXL02942.1"
                     /translation="MKEAEVVVIGGGPAGYAAATRAGREGWKTILIEGRELGGTCLNR
                     GCIPTKAFFKSQEVTNLVRKASDFGIKADFQGIDWEKVLNRKNRVVKQLTSGVQFLLQ
                     KGKVEIIKGFASFLDSHTVRVEKEGGEKEEIRGKYIILASGSRSATLPVEGTNLEGVI
                     DSDQALELSSLPESLVVIGGGVIGMEMACIFNAFGVKVEVVEMMPKILPPVDEEITNL
                     LTQLVEKRGMKVHVNAQVKKIHRSGNLRVTFLQEGEEKEVEGDYVLVATGRVPQVENL
                     GIENLGLKMENRGVWTDEFLRTSIPHLYAPGDVNGKYLLAHVAYREAEVVISHIKGEN
                     SKINYKAIPNCIFSFPEIASVGLTEKEAEAKGYEVKVGKFPFRANGKALIEGEIDGLV
                     KMVSKAKNGEILGVHIIGPHASDLIGEAVLALNLECTPQEIAETIHPHPTLSEALMEA
                     AQGVFTSPLHFS"
     gene            complement(3797..5209)
                     /gene="gltA"
                     /locus_tag="PK016_07515"
     CDS             complement(3797..5209)
                     /gene="gltA"
                     /locus_tag="PK016_07515"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HXL02943.1"
                     /translation="MSPERPSRPKMPQRPPEERIKDFNEVPLGLSEEAAVTEARRCIQ
                     CKKPTCVRGCPVEIDITGFIKLLSQGKFDEAIAKIKEKNSLPAICGRVCPQEDQCESQ
                     CILGKRGQPIAIGYLERFLADYEREKGVKAPSLPPPREEKVAIIGSGPAGLTCAGDLA
                     KMGYKVTIFEALHAPGGVLVYGIPEFRLPKTIVAQEVNYVRSLGVELETSRVIGKTFT
                     LDDLKDEGYQAFFIGTGAGLPYFLEVPGENLNGIYSANEFLTRSNLMKAYLFPRYDTP
                     VKVGQRVAVIGGGNVAMDSARTSLRLGAKEVCLVYRRTKKEMPARIEEVERAEEEGVN
                     CIILTNPVRFLGNENGWVTGIECIQMELGEPDESGRRRPVPIPGSEFVIPIDTVVVAI
                     GQGPNPLLLETIPGLQLNKRGYIVADSETGATSLPGVFAGGDIVTGAATVISAMGAGK
                     RSAQAIDRFFTDPDSFPQWR"
     gene            complement(5199..>5450)
                     /locus_tag="PK016_07520"
     CDS             complement(5199..>5450)
                     /locus_tag="PK016_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HXL02944.1"
                     /translation="YSIPTLVSLNPIMVDGTGMCGCCRVTVGGKCKFTCVDGPVFDGH
                     QVNFDELMARQRIYLEEEKRALDSWLMEKEKEEATYHVS"
BASE COUNT         1533 a         1364 c          896 g         1657 t
ORIGIN      
        1 tcgacgggat ttgaacccgc gatcttcaac gtgacaggct gacgtgttag gccagactac
       61 accacgaccc cacatgctta cattttaata gctaaaatca tttttggcaa gaggcaaaac
      121 tgaattcagt gtttatcttt ctgtgtgata cagagaatca ggataatatt tagtacaaaa
      181 gagctcttct gctttttgta actcttcagc actccactct ccttcttcta tctctacacc
      241 ccactcttcc tctaaactct ccaaaagcaa tcgataaatt tcctttttct caggtaagga
      301 agaaagaatt tcattaagag gaagaaattt attttccagc tctttttgaa ccttttcttt
      361 ttccttaaaa caagaagcaa tcttttctct atctccttcc aaaataaaag aagcttgaaa
      421 gagattccct ttctctcctc gagcttgagc tactccaata attttcttcc ctcttaccat
      481 tatctcatgg gaaacatgaa tagaaaagca agaaggggaa gaagtataaa atcccgattc
      541 taccttttca tctactttta tgcccatctt tcctatcatt cgggttagaa ctttttgcaa
      601 ttcctcataa taaaaagtat gtctcttaaa aggagaagcg gggataaata aactgaaggt
      661 aacctcctta tcatgcaaaa tagctcttcc cccggtaggt cggcgcacca aaggaaggtt
      721 tttagtttta aggtagtcaa aatctatttc cctggttttt tggaatctcc ccaccgaaag
      781 ggttttttcc ctccactcaa agaaacgcaa agtcaaaaga gcatttgaag agcctatctc
      841 gagaagtgcc tcatctctac tcatattttc ataaccagag agaggggagt cagtaagaac
      901 tctaactatt ttcctcattg agtcttaaga aatgcttcgt attcctcttt actcatcaaa
      961 ttcttaaatt cttcttcgtt ttccggttga actttacaaa tccaaccctc tcgataagga
     1021 ctttgattga ttaactccgg ctttttatct aattccttat ttacttccac tacttcccca
     1081 gaaaggggag catataaata agaaactgtt tttatagact ccacttctcc tattctatct
     1141 ccttgcttta ttttcttccc tacatctggt aactctacaa atactacatc tcctaactca
     1201 ttttgagcgt agtcagtaat cccacaagta accacatctc ctttttttac ccaaaaatgt
     1261 tcccgggtgt aaaataaatc atcaggaatg ttcatattta ttaacctcct tccccgaagg
     1321 gtaaactcac catttttgct tccaatcgag ttcggggtgc agctaccaac aaattttctc
     1381 ctcttccgct acaagcggag ctaacaaacc ccagagctaa aactttatgc caggaaaaag
     1441 aataattacc actggttaca tagccacaag gcatcccttc tttagtataa aggggataac
     1501 cataacgagg aattctccgc cccagaatct ctaaccctac cagtttcttt ttagaacctt
     1561 cttccctttc taaaagaaga ggctttttac caataaaatc aatcttttcc caatctatga
     1621 ggtcctctct acctagttcc agtggagtaa tagtttcgtc tatctcctga ccataaagag
     1681 gaaaagtggc ttcaaagcgc aagagatctc ttgcgcctaa cccacatagt gcaccccctt
     1741 ggctttccac ctcttctacc agttttttcc accattccaa acccaaagat acatcgctca
     1801 ttatctcaaa cccatcttcc cgggaaaaag caagatggat tatctgaaac ccaggagaga
     1861 attgccgaaa agaaaacttt ggccaattat cattgggaac cgggaacaat tgcctcactg
     1921 tttgttgggc ttgaggacct tgcagagcaa aaagcgtctt tttttccgtt acatcttcta
     1981 cttctacttc ccatttctgg ttctttatca gataatcaag ccactggaaa agtttttccc
     2041 gtgccccggc attggaaatc aaaagccact cttcttcttt ttccctgaaa atagttactc
     2101 catccactat cccacctcgc cagttgagga aaaaagtgta tttacctttc ccttccttaa
     2161 ctttagaaac ttggcggcaa gtagcataat ctaaaaaagg agttacttct ttgcctcgaa
     2221 gaaaaaattt ccccagatga gaaatatcaa agagtccaca actattgata cagctaaaat
     2281 actcttcccg gatactacga taataaagag gcatcttcca gccagaaaat actcccatcc
     2341 gagcccccaa agagcgatga aaattagaaa gaggtaactc cttaagcgtc atctaatttt
     2401 cctttaagag aaatgcaagg gggaggtaaa aactccttga gccgcttcca ttaaagcctc
     2461 agaaagtgtg ggatgaggat gaatggtctc agctatttct tgaggagtgc attctaaatt
     2521 tagagctaaa actgcttctc caatcaaatc tgaggcatga ggtcctatga tatgcactcc
     2581 cagtatttct ccatttttag ctttgctgac catttttacc aggccatcaa tttccccctc
     2641 aatcaaagct ttaccatttg ctcgaaaggg gaatttccct actttaactt catatccctt
     2701 tgcttctgct tccttctcag ttaaacctac agaggcgatt tctggaaaag aaaaaatgca
     2761 gttgggtatt gctttataat ttattttact attttctccc ttaatatgac ttatcactac
     2821 ttctgcctcc ctgtaagcta catgagctaa gagatatttc ccattgacat ctcctggagc
     2881 ataaaggtga ggaatagagg tacgcaaaaa ttcatccgtc caaacacctc ggttttccat
     2941 ttttaaaccc aagttttcta tccctaaatt ttctacctga ggcactctcc ctgtagccac
     3001 taaaacgtaa tctccttcta cttctttttc ttctccttct tggagaaagg ttactcgcaa
     3061 gttccctgag cgatgaattt tcttcacttg agcattgaca tgaactttca ttcctctttt
     3121 ttctaccagc tgagtaagaa ggttggttat ttcctcatcc acgggaggaa gaattttggg
     3181 catcatttcc accacttcta ccttaactcc aaaagcgttg aagatgcagg ccatctccat
     3241 ccctataact cctcccccga taactaccag actctcagga agagaagaaa gttccagtgc
     3301 ttggtcacta tcgatgaccc cttctaaatt agtcccttct accgggagag tagcagaacg
     3361 tgaaccacta gccaaaatta tatacttacc ccggatttcc tccttttctc ctccttcctt
     3421 ttctaccctt acagtatgag agtccagaaa ggaggcgaaa ccttttatta tctccacttt
     3481 gcctttctgg aggagaaatt ggactccact ggttagttgt tttaccaccc gattttttcg
     3541 attcaggact ttctcccaat cgattccttg aaaatccgct tttattccaa aatctgatgc
     3601 ttttcttacc agattggtta cttcttgaga tttaaaaaag gctttagtag gaatgcaccc
     3661 ccggttaagg cacgttcctc ctaattctct tccttcaata aggatggttt tccacccttc
     3721 tcgtcctgct cgagtagccg cagcataacc tgccgggcct cctccaataa ccacaacttc
     3781 tgcttctttc attttattac ctccactgag gaaaactatc agggtcagtg aaaaaacggt
     3841 ctatggcttg cgctgacctt ttacccgctc ccatagctga tattactgtg gctgctccag
     3901 ttactatgtc ccctccggca aaaacccccg gtaaactagt agctccagtt tctgagtcag
     3961 caacgatgta acctctttta ttcaattgca aaccaggaat ggtttctaaa agtaaaggat
     4021 taggaccttg acctatggct accactacag tatcaatagg aataacaaat tctgaaccgg
     4081 gaatgggaac gggtcttctt cttcctgact catctggctc tcccaattcc atctgaatac
     4141 attcaatacc cgttacccaa ccattttcat tacctaaaaa acggaccgga ttagttaaaa
     4201 taatgcagtt tactccctct tcttccgctc tttccacttc ttcaattcga gcaggcattt
     4261 cctttttagt acgtcgatat actaaacata cctcttttgc tcccaggcgc agggaagttc
     4321 gagcagaatc catagctaca tttccccctc caataactgc tactctttgc cctactttca
     4381 caggagtgtc atagcgagga aagagatatg ctttcatcaa gttggaacgg gtgagaaatt
     4441 cgttagcgga ataaatacca tttaaatttt cccccggaac ttctaaaaag tagggaagac
     4501 cagctcctgt tccaataaag aaagcctggt atccttcatc ttttaagtcg tccagagtaa
     4561 aagttttacc tatcaccctg ctggtttcta attctactcc caaagatcgc acataattga
     4621 cttcttgtgc cacaatggtt ttgggaaggc ggaattcagg aataccataa actaaaactc
     4681 cacctggagc atggagagcc tcaaaaatgg taactttata acccattttg gccaaatccc
     4741 cagcgcaggt taaacctgca ggacctgagc caattatcgc tactttttcc tctcgaggag
     4801 gaggaagaga aggagccttt actccctttt ctctttcgta atcagccaag aaacgctcta
     4861 agtatccaat ggcaataggt tgtcctcttt tacccagtat gcattgactt tcacactggt
     4921 cttcttgagg acacactctt ccacatatgg cagggagact atttttctct tttattttag
     4981 caatagcttc atcaaattta ccctgagata gaagcttgat aaaaccagta atatcaattt
     5041 ccaccggaca acccctgaca caggtaggtt ttttacattg gatacaacga cgagcctctg
     5101 ttactgctgc ttcttccgat aaccccaggg gaacttcgtt aaaatctttg atgcgttcct
     5161 caggaggacg ctgaggcatt ttaggtctag aaggacgttc aggagacatg gtaagtagct
     5221 tcctcctttt ctttttccat tagccaggaa tctaacgctc gtttttcttc ttccaaataa
     5281 attcgctgac gagccatcaa ctcatcaaag tttacctgat ggccatcaaa aaccggacca
     5341 tctacacagg taaacttaca ctttcctccc actgttaccc ggcagcaacc acacattccc
     5401 gttccatcca ccattatggg attaaggctc accagagtgg gaatggaata
//
LOCUS       DAOSVE010000101         5384 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_6850_length_5384_cov_69.624883, whole genome shotgun sequence.
ACCESSION   DAOSVE010000101 DAOSVE010000000
VERSION     DAOSVE010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 5384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5384
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6850_length_5384_cov_69.624883"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            347..1807
                     /locus_tag="PK016_07525"
     CDS             347..1807
                     /locus_tag="PK016_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HXL02945.1"
                     /translation="MNQPFRRYLRWLYPGMRVKRWLLLVIVGVFLISLGVSLIVSTPY
                     FRSIYALWRNFVFGFLNRYWLAVVFGLAWLAGGVALAVYGLQRMNRSVITAISSLPEK
                     EVVNEVFKKRQLEKGPSIVAIGGGHGLYSLLHGLKEYTSNTTAVVTVFDDGGSSGRLR
                     EEMGVLPPGDIRNCLIALADAEPLMSELFQYRFGDDTGLGGHSFGNLFVTALAQITGD
                     FQKAVFESSKILAVRGRVLPTTIQNVFLRAECEDGSIILGESQVGTCGKKIKRIFLEP
                     SPVHTTPEVIKAIEEADLIVLGPGSLYTSVLCNLAVEEVREAILRSPAPLVFVCNITT
                     QPGETDGYDFSDHLQAVFQFILPQRLNYILVNNGAPSEAGEEELSLSGAEMVQGNGEE
                     LPSSIKVIEADLISSHHPSRHDSQKLAQALMSILLEEKPSWAFYLTLHNDEILKNLNL
                     VHLVNHWRVKVRENGGKKEKRENSTPVYGLKASRRK"
     gene            complement(1777..3342)
                     /locus_tag="PK016_07530"
     CDS             complement(1777..3342)
                     /locus_tag="PK016_07530"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXL02946.1"
                     /translation="MPDRYDVLIIGNGIAGSSAALFLAEAGKRVLLLTKGKGLEDTNT
                     AKAQGGIVFRGEGDRPSFLLRDIMEASAFTSYTASARLTSRLGPPLVQKLLIDRLQIP
                     FDRRNGEWDLFQEGNHSLRRILHIKDYTGKVIQEALNKAVLREPLIEVKKDSLVLDLI
                     VSSRDTPDPENRYLPLECLGAYVWEGETQEVVPAFASATIVATGGLSEIYYYSSGGPW
                     NTGDGFAIAHRAGARLSNMEYVQFHPTLLYTPQYSSSLLISESARGEGAELINADGEP
                     FMFRYHPRGSLAPRDVVARAILQEMKIHESNCVYLDFRQKMTPDKIKDTFPTIYEELL
                     RRGIDITQEPAPVAPGAHFSCGGVLTDTHGRTTIKRLYAAGEVACSGLHGANRLASTS
                     LLEGLTFGYRVARDIVKHSEELPNFTVLPFRGKWGEPPPENILRSLKEIIQINMWENV
                     GLIRERKGLSQSLQILTTLKKEIAHLKEEYGISPVLLELENMAETALLVTEEALKNPL
                     SRGTHFRLDAFNP"
     gene            complement(3335..4186)
                     /gene="nadC"
                     /locus_tag="PK016_07535"
     CDS             complement(3335..4186)
                     /gene="nadC"
                     /locus_tag="PK016_07535"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HXL02947.1"
                     /translation="MLGLVPWDVDRRLQAFLEEDLGFDDITTQGLGDLGKNIVKARIT
                     AKKEGIMAGGFFALRVFALLDPRTRTEAKVPEGQHFSPGEVLLEIEGPARAILMGERT
                     ALNLLQRLCGIATKTQELVKLVEDLPVKVADTRKTSPGLRIFEKYAVRCGGGVNHRLG
                     LYDCALIKDNHIALCGSVKEAIKKIRPALPFTAKIVVECTTLVQVEEALQGKADVIML
                     DNMSIETIREAVKLAKGKATLEASGDINPTNIRKVAEQGVDIISSGYITHHATWIDMS
                     LKIKENA"
     gene            complement(4201..5247)
                     /locus_tag="PK016_07540"
     CDS             complement(4201..5247)
                     /locus_tag="PK016_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02948.1"
                     /translation="MTCFLFVTRGELEEKLSLALYHKMKETYPEVKILFFSIDNPGIW
                     QEKGLAEIKPPRSSFRNAMSGFKKLTFTYSALAKRRNFKEKVVLWSDNPSFLLPLFLL
                     TGKKAIYIPVIKSLRFSLSFWHTIFFRFSSVVLTNDRELVHNLRRKNMPAYFVGNLLV
                     DLLSSSVFLFPHGRKPIYALFPREENFSSDLKFFLDVVEKISSEVKAYFILAIPRKIK
                     TQEAREAGEKKGWHWRRSLEGDTMEGYLEKEGIYLNLTRFRGEALRQCNLALSIDDFS
                     TIQATGAGRKVLPISNLNPDEVVSLMRNPQYLFEYNQYLSSRYGREGGIEKLSTYLLQ
                     GIVEDENFLKKFSK"
BASE COUNT         1422 a         1125 c         1211 g         1626 t
ORIGIN      
        1 ggagagggtt agggtgaggg tgtcttcttg ccgttattcc tgagtgtatt tatcatcccc
       61 ttgtctgtca ttcctgaatg tatttatcag gaatctgaac tatgaggggg cttgccccct
      121 catagtcgat agaacaattc aggggcaggc taaaaacggc tcgaaagttg ccggggtgat
      181 ggatgaggag ctttattgcg gcgacagaag agggttataa ggagggtttt tctcctttta
      241 tagtttgtgg taaaattata cctggcaaat gaaaaagagg ttaaatatac tgataccttg
      301 agagtttata taaaatttta tcctggtgtc taagggggaa aggttgatga atcaaccttt
      361 taggcgatat ctgcggtggc tgtacccggg aatgcgggta aagcgatggt tgcttctggt
      421 tattgtgggt gtgttcctca tctctttagg agtgagttta attgtttcta ctccctattt
      481 tagaagtata tatgcactgt ggagaaactt tgtcttcggg tttttaaacc gttactggtt
      541 agctgtagtt tttggtctgg catggttagc gggaggtgtg gcactggcag tctatggctt
      601 gcagagaatg aaccgttcgg ttattactgc catttcttct cttccggaaa aagaagtagt
      661 taatgaagtt ttcaaaaaga gacagctgga aaaaggacca agcattgtgg ctattggagg
      721 agggcatgga ttatattctc ttctccatgg actaaaagaa tatacttcta acaccacagc
      781 agtagttaca gtttttgacg atggaggaag ttctggtcgc ctgcgagaag aaatgggagt
      841 tttgcctcct ggcgatattc gcaactgttt gatagcttta gctgatgctg agcctttgat
      901 gagtgaacta tttcagtatc gctttgggga tgataccggg ttgggaggac atagttttgg
      961 caatctcttc gttactgctc tggctcaaat tactggagat ttccaaaaag cggtttttga
     1021 atccagcaaa atcttagcgg tgagagggag agttttgcct actactatcc agaatgtctt
     1081 tctccgtgct gaatgcgaag atgggtcaat cattctggga gaatctcaag taggtacctg
     1141 tgggaagaaa ataaagagga tttttctgga accctcaccg gtgcatacca ctcctgaggt
     1201 gattaaagcc attgaagaag cagacttgat agtgttgggg ccgggaagtc tgtataccag
     1261 tgttttatgt aatttagcag tggaggaagt gagagaggct attttgcgtt ctcctgctcc
     1321 tttagtcttt gtgtgcaata tcactactca gccaggagaa acggatggct atgatttttc
     1381 tgaccatttg caagcggtat tccagtttat tcttcctcag cgcttgaact atatcttggt
     1441 aaataatgga gcacccagcg aagcggggga agaggagcta tctctctcgg gggcagagat
     1501 ggttcaggga aacggtgaag agctaccttc ttccatcaaa gttatagagg cggaccttat
     1561 ttcttcccat catcccagtc gtcatgattc ccagaagtta gctcaagcac ttatgtctat
     1621 tcttctggaa gaaaaacctt cctgggcttt ttatttgact ctccacaacg acgaaatcct
     1681 caagaatttg aaccttgtac atcttgtaaa ccattggagg gtaaaagtac gggaaaatgg
     1741 aggaaagaaa gaaaaaaggg agaactctac ccccgtttat ggattaaaag cgtccagacg
     1801 gaagtgagtt ccccgggata aagggttttt taatgcttct tctgtcacca aaagagcggt
     1861 ttcggccata ttttccagtt ctaaaaggac cggagatatg ccgtattctt ctttgaggtg
     1921 agcaatttct ttttttagag tggtcaatat ctgtaaagat tgggaaagtc cctttctttc
     1981 ccttattaat cccacatttt cccacatatt tatctggatg atttctttca ggctgcgtaa
     2041 aatgttttct gggggaggtt ctccccattt ccctcgaaaa ggaagaacag taaaattagg
     2101 aagctcttca ctatgtttta ctatatcccg agctactcga tatccaaaag tgagtccttc
     2161 aagtaaactg gtggaagcta aacgattagc tccgtgcaaa ccactacaag ctacttctcc
     2221 tgcagcgtac aatcttttta tggtggttct gccatgggta tcggtgagca ctcctccaca
     2281 ggaaaaatga gcaccaggag caacaggagc tggttcttgg gtgatatcta ttccccttcg
     2341 gagaagttct tcataaatgg tggggaaggt gtctttaatt ttatcgggag tcattttttg
     2401 ccgaaagtct aaataaacac aattactctc gtgaattttc atttcctgta aaattgccct
     2461 tgctaccaca tcccgggggg ctaaacttcc ccgggggtga tatcgaaaca taaaaggctc
     2521 tccatctgca tttatgagtt ctgctccttc tcctcgagct gattcagaga taagaagaga
     2581 ggaagaatat tgaggagtat aaaggagggt ggggtgaaat tgcacgtatt ccatattact
     2641 caggcgagcc ccggctctgt gggctatagc aaaaccatct cctgtattcc aggggcctcc
     2701 tgaagaatag tagtaaattt cactcaatcc cccggtagct acaatggtag cggaggcaaa
     2761 agctggaact acttcttggg tttctccctc ccacacataa gctcccagac actccagagg
     2821 tagatagcga ttttcagggt ccggagtatc acgggaagag acgatgaggt ctaacaccag
     2881 agaatctttt ttcacctcaa ttaatggttc gcgtaagaca gctttgttca aagcttcctg
     2941 aataactttt cctgtgtaat ccttaatgtg gagaatacgg cgaagggaat ggtttccttc
     3001 ctggaaaagg tcccattcgc cgttccttcg gtcaaaggga atttgcaaac ggtcaattaa
     3061 taatttttgt acgagaggtg gccccaagcg agatgtcagt cgagcgctag cagtataaga
     3121 tgtgaacgcc gaggcttcca ttatgtctct aaggagaaaa gagggacggt cgccctctcc
     3181 ccggaatact attcctccct gggctttagc ggtgttagta tcctctaaac ctttgccttt
     3241 agtgagaagt aacactctct ttcctgcttc agctaagaag agagcagcac tacttccggc
     3301 aatgccgtta ccaatgatta agacatcata acggtcaggc attttctttt attttcagac
     3361 tcatatcaat ccaggtagca tggtgggtga tgtaaccgct ggaaattata tctactccct
     3421 gctctgccac tttacgaatg ttggtggggt taatatctcc ggatgcttcc agagtggcct
     3481 tccctttagc taatttaact gcttcccgga tagtttctat actcatattg tctaacatga
     3541 tgacgtctgc ttttccttgt aaagcttctt ctacttgcac taacgtggta cattctacca
     3601 ctattttggc ggtaaaagga agagcagggc ggattttttt aattgcttct ttcactgacc
     3661 cacacagagc gatgtggtta tctttaatta gggcgcagtc atagagcccc aaacggtgat
     3721 ttactcctcc tccacatctc acagcatatt tttcaaaaat ccgcaacccc gggctggttt
     3781 tccgagtatc ggctactttc accggcaggt cttctaccag ttttaccagt tcttgagttt
     3841 tagtggcgat accacagagg cgttgcagga ggtttaaggc agttctttcc cccatgagaa
     3901 tagctcgtgc tggcccttct atttctagca gcacttcccc gggagaaaaa tgttgtcctt
     3961 caggaacttt tgcttcagtt cttgtccggg ggtcaagaag ggcaaacact cgaagagcaa
     4021 aaaaccctcc ggccattatt ccttcttttt tggccgtaat cctggctttt actatatttt
     4081 tccccaggtc gcccaatcct tgagtagtga tatcatcaaa tcccaggtct tcttctaaga
     4141 aggcctgcag tcttcggtct acatcccagg gtactaatcc tagcatattc atcacccttt
     4201 ctatttggag aatttcttga gaaaattctc atcttccact atcccctgca agagataggt
     4261 ggataatttt tctattcctc cttctcttcc ataccgggaa gagagatatt ggttatattc
     4321 aaaaagatat tgagggttac gcattaaaga gaccacctcg tcggggttga ggttagaaat
     4381 agggagcact tttctacctg ctcccgtggc ctggatggtg gaaaaatcgt cgatacttaa
     4441 agctaaattg cattgtcgta gtgcttctcc tctaaagcgg gtaagattca aataaattcc
     4501 ttctttttct aaatatcctt ccatggtatc tccctctaaa cttctcctcc agtgccatcc
     4561 ttttttctct cctgcttctc ttgcttcctg ggtttttatt ttcctgggga tggctaagat
     4621 gaagtaggct ttaacctccg aagatatttt ttctactaca tctaaaaaga actttaaatc
     4681 agaagaaaaa ttctcttctc tagggaacag ggcataaatg ggttttctcc catggggaaa
     4741 taggaataca gaagaggaaa gaagatcaac taagagatta cccacaaaat aggctggcat
     4801 atttttcctc cgtaagttgt gcactagttc ccggtcatta gttaaaacta cggaagaaaa
     4861 gcgaaagaaa atggtatgcc agaaggatag agaaaagcga aggcttttaa tcaccggaat
     4921 gtatatagct tttttaccgg ttaaaagaaa aagaggaagg agaaaagagg gattatcgct
     4981 ccagagtacc actttttcct taaaatttct cctctttgcc agagcagaat aagtaaaggt
     5041 taatttcttg aatcctgaca tggcgtttct aaaggaagac cgaggaggtt ttatttctgc
     5101 caatcctttt tcttgccata tccccgggtt gtcgatactg aaaaagagga tttttacctc
     5161 aggataagtt tccttcattt tatgataaag tgccagggat agtttttctt ctaattctcc
     5221 ccgggtaaca aatagaaaac aagtcattac cgccaccttt tctaagttta taattaaccg
     5281 caaaatccat tataatgact atgaggggga aaagaccccc tttataaatc ccctttcttt
     5341 ttcttccctc gccttcaatg gaagaacccc acatattcct tccc
//
LOCUS       DAOSVE010000102         5282 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_6986_length_5282_cov_63.890568, whole genome shotgun sequence.
ACCESSION   DAOSVE010000102 DAOSVE010000000
VERSION     DAOSVE010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 5282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5282
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6986_length_5282_cov_63.890568"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="PK016_07545"
     CDS             <1..617
                     /locus_tag="PK016_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02949.1"
                     /translation="PFVVTFEDVLRCKKRSCEFYIERPEPVECGDTVLVWGDYFGAAD
                     AVEKLGFEGKKVFVVTKNKEFASWMEPCHRDVMMKRMAGGNGEGLKTRTYAHPVTVIT
                     ESTVVEIKSNGEVVIEDREFNKSTIKVDNVVLAQVECNDDLYQQLLDAGMVVAKIGDA
                     KKVRNLRGAVTDGANIGLTLDLDLMMNANNSPIANLPTEVKVSG"
     gene            925..1497
                     /locus_tag="PK016_07550"
     CDS             925..1497
                     /locus_tag="PK016_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010870005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HXL02950.1"
                     /translation="MDVQVTYFDQKGPVNTEATLKIAQKRAKELGIEQVVVASTYGET
                     ARKALQIFDSSRVNVVVVTISYGFKSEGWIMEEEVRRELEEKGAKVLTSIHALGDDVS
                     SSFSSQKGGWGANQIVAEALRRFSQGMKVAVEITLMAAEAGLIDVEKETISIGGTDRG
                     VDTAIVVKPAYARNFLDFEIREILAKPRIG"
     gene            complement(1522..>1732)
                     /locus_tag="PK016_07555"
     CDS             complement(1522..>1732)
                     /locus_tag="PK016_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02951.1"
                     /translation="SFLNVSIRNLKQNYKGETTPYYLLRKIKEKTKNIKEWKTTEGDK
                     SSLSKTFGFPIEDFGNDKRRRIPDY"
     assembly_gap    1733..1832
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1951..2748
                     /locus_tag="PK016_07560"
     CDS             1951..2748
                     /locus_tag="PK016_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXL02952.1"
                     /translation="MDEKANLLPKSEKLTEDSNIPLYHQLKNIIKAQIITGAIQPGDK
                     IPSESELGERYGVSRITVRQAINSLAQEGYLYRKQGVGTFVTSPKLRRRLPRLYSFSE
                     DMRELGLEPSSQVLEQKVIEANEEMAEILKLPPGDRRVNKLVRVRLANESPILLEKTF
                     IPYYLCPDLTKEDLEKGSLYSLLREKYNLLLDHAFETYEVGKIRKEEAKVLKCPVGLP
                     AFVIERLTYLKTEVPVEWTKSVARGDRLRFTVRLVADQAQIRREIEF"
     gene            2759..3745
                     /locus_tag="PK016_07565"
     CDS             2759..3745
                     /locus_tag="PK016_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HXL02953.1"
                     /translation="MVKTIFLGIDLGGTNTKVALVDSEGTILERSIIPTRAMRKAEEV
                     VKDIASEAMKLRKRFEERGFKVQAAGIGIPGLIDWEKGICLLLPNFPNKWRDVPIREW
                     LEEELSLPVAVINDVRAITLAEKRFGAGKDVQSLVMVAIGTGIGGGVVLDGDLYIGKD
                     GAAGEFGHITVEPNGVRCGCGSRGCLEAYSSGPAMVAQALRALVQQNDTLIRDLVGDD
                     LSRVNPKVIAEAAQKGDKVATEIIERAGSYIGQALSCICLVVNPEMIVIGGGVAQAGE
                     LLFRSIEQGLRERLFMIPVDTIQFVPAQLGMDAGAVGTATWAKERLKKGAIG"
     gene            3742..4893
                     /locus_tag="PK016_07570"
     CDS             3742..4893
                     /locus_tag="PK016_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXL02954.1"
                     /translation="MSDTLKVGVIGTGFIGPAHIEASRRTFLAEVVSLADINEEVAHQ
                     KANQYAVRKYYGDYQNLLQDEEVEVVHVCTPNYLHYQMSKDALLAGKHVICEKPLAMN
                     RQEAEELVELADKKGLVNAVHFNIRYYPLAREAHEMVKGGEVGKVLAVHGSYLQDWLF
                     YETDYNWRLEPQLSGDSRAIADIGSHWLDLVEYVTQKKIAEVFADFATFHKTRKKPLK
                     PVETYAGKILQPEDYQDVPISTEDYASVLLHFEDGAHGVLTVNQVAAGRKNRCSFEID
                     GSRSALFWDSERPNELWIGRRDRANELLLKDPSLVSEETRKIISFPGGHNEGFPDTSK
                     QLFREIYQYILEGKRENSSFPTFKDGLREVVLCEAILHSAKEEKWVKVN"
     gene            4909..>5282
                     /locus_tag="PK016_07575"
     CDS             4909..>5282
                     /locus_tag="PK016_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HXL02955.1"
                     /translation="MKLGFMTACLPEMKLEEIVKWAKDKGFGMLEVACWPRVFEKRRY
                     AGTQHIDVENFTEEKAQEIKELFRNNHLEISSLGYYPNNLDPDLESRRFYHEHLKKVI
                     QAAEKLGVKVVGTFVGRDPRMNV"
BASE COUNT         1611 a          805 c         1313 g         1453 t
ORIGIN      
        1 taccttttgt agttaccttt gaggatgttt tgcgatgcaa aaagagaagc tgtgagtttt
       61 atattgagag accagagcca gtagaatgcg gtgatacagt tttagtttgg ggagactatt
      121 ttggtgctgc agatgcagta gaaaaattag gctttgaggg aaagaaagtt tttgtggtaa
      181 ctaagaataa agagtttgct tcctggatgg aaccttgcca ccgtgatgta atgatgaaga
      241 ggatggccgg aggaaatgga gagggactta aaactcgaac ttacgcccat ccagtaacag
      301 taataactga atctacagtg gtagaaatta agagtaacgg agaagtagtg attgaggacc
      361 gagagtttaa taagtctacc attaaagtag ataatgtggt tttagctcaa gtagaatgca
      421 acgatgattt gtatcagcaa ttgctcgatg ccggtatggt ggtagctaag ataggagatg
      481 ccaaaaaagt gagaaacctg aggggcgcgg ttactgatgg ggcaaatatc ggccttactc
      541 tggatttaga tttaatgatg aatgccaaca atagccctat tgctaatctt cctactgagg
      601 taaaagtttc aggctgatta aaattttttt ctctatccag ccctaaacta taccttatgg
      661 tttagggctg gatttatata gtattatgca gaacctgtta taattataat ataagcagga
      721 aaaaagagag ttatcgcaca atagctgtga catgagcgaa aaggcgtggg aaactaagac
      781 tacctgcggt gtaagataaa agatatcaaa aacataaaag acgaaaatca ccaggggagc
      841 aaacctctcc ggtagcgctg ctatacttta agaaatagca tttaccatgg tagaatcttg
      901 attgagttaa agggggtagt gaatatggat gttcaagtaa cttattttga ccagaagggg
      961 cctgtaaata cagaggcaac tttaaaaatt gcccaaaaaa gagcgaaaga gctgggaatt
     1021 gaacaagtag ttgtagcttc tacttatgga gaaacagcca gaaaagcttt acagattttt
     1081 gatagctcta gagtcaatgt agtagtagta accatctctt atgggtttaa gagtgaaggc
     1141 tggataatgg aagaagaagt tagaagagaa ctagaggaaa aaggggctaa agtacttact
     1201 tctattcatg ctttgggaga tgatgtgagc tccagtttta gttctcaaaa aggaggctgg
     1261 ggtgctaacc agattgtggc tgaggctctt agaaggttca gtcagggtat gaaagtggcg
     1321 gtagaaatta ccttaatggc tgcagaggcc gggcttatcg atgtggaaaa agaaactata
     1381 tccataggag gcactgatag aggggtagat accgctattg tggtgaaacc tgcttatgcc
     1441 agaaactttt tagatttcga aatcagggag attttggcta aacccagaat tggatgacga
     1501 actttctttt cattcccgaa atcagtaatc gggaatccgt ctccttttgt cattcccgaa
     1561 atcttcaatc gggaatccaa aagtcttgct gagggaggat ttgtctcctt cagtagtttt
     1621 ccattctttt atgtttttag ttttttcttt gatttttctc aggaggtaat agggagttgt
     1681 ttctccttta tagttctgtt ttagattcct gatagaaaca ttcaggaatg acnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnacattcag gaatgacagg caagggaagg
     1861 gggcttgccc ctttatagtt ctgttataat ctttatgcag tgtttatatt ataatgattc
     1921 taaaaggtgt gtaaacgatg aggaaaaaga atggatgaaa aggctaatct tttgcccaaa
     1981 tcagaaaaat taacagagga tagtaatatt cctctttatc accagttgaa gaatattatc
     2041 aaagctcaaa ttattaccgg tgctattcag ccaggagata aaattccttc tgaaagtgaa
     2101 ttgggtgaaa gatacggagt aagtaggata acagtaagac aggctatcaa ctctctggca
     2161 caggaaggtt acctgtatcg caagcaagga gtggggactt ttgttacttc tcctaagctg
     2221 cggcgaaggc ttcctcgact ctacagcttt agtgaagaca tgcgagaatt agggctggag
     2281 cccagctctc aagtattaga acagaaggta atagaggcta atgaggaaat ggcagagatt
     2341 ttgaaattgc ctcccgggga tcgaagagtt aacaagttgg ttagggtaag gttagctaat
     2401 gaatcgccta tcttattaga gaaaactttt attccttatt atctttgtcc tgacttgact
     2461 aaagaagatt tagaaaaggg ctctctttat tctttgttga gagaaaaata caatcttttg
     2521 ctcgaccatg ctttcgaaac ttatgaggtg ggaaaaattc gcaaagaaga ggctaaggtt
     2581 ttaaagtgcc cggttggtct acctgccttt gttattgaaa gattgactta tttaaagact
     2641 gaagttcctg tagaatggac taaatcggta gcaagaggag accgcttgcg gtttacagtg
     2701 aggttggtag ccgatcaagc tcaaattcgc cgagaaatag aattttagga ggttttatat
     2761 ggttaaaaca atttttttgg ggatagactt aggaggaact aataccaaag tagctttagt
     2821 ggatagtgaa ggaacaattc tggaaagaag tatcattccc actcgagcta tgcggaaagc
     2881 agaagaagta gtaaaagata ttgcctcaga agctatgaag ttgcgaaaaa gatttgagga
     2941 gcgaggtttt aaagtgcagg ccgcgggtat cgggattcct ggtcttattg actgggaaaa
     3001 agggatttgc cttttacttc ctaattttcc caacaaatgg agagatgttc ccatccggga
     3061 gtggttagaa gaagagctgt ctttaccggt agcagtaatt aatgatgtga gagccataac
     3121 tttagctgag aaaaggtttg gagcgggaaa agacgtacag agcttggtga tggtggctat
     3181 cggtacagga attggaggag gagtggtatt ggatggtgac ctctatattg gaaaagatgg
     3241 agcagcaggg gaatttggac acatcacagt agagccgaac ggagtacgct gtggatgtgg
     3301 tagccgaggg tgtttagaag cttattcttc aggacctgct atggtagcgc aagctttgcg
     3361 agctttagtt cagcaaaatg atactctaat tcgagatttg gtaggagatg accttagtcg
     3421 ggttaatccc aaagttattg ccgaagctgc tcagaaaggg gataaagtgg ctacggaaat
     3481 aattgagcga gctggttctt acattggcca ggctctttct tgtatctgtc tggtagttaa
     3541 tccggagatg atagttattg gaggaggggt agcacaggct ggagaattac tttttagaag
     3601 tattgagcaa ggtttacgag agagactttt tatgattcca gtggacacta tccaatttgt
     3661 tccagcccaa ttaggtatgg atgcgggtgc tgttggtacg gctacctggg caaaagaaag
     3721 gttaaaaaag ggggctatag gatgagtgac actcttaaag tgggagtaat aggcactggt
     3781 tttattggtc cggctcacat cgaagcttct cggcggactt ttttagctga agtggtgagt
     3841 ttagctgata taaacgaaga ggtagctcac caaaaagcca atcaatatgc agtaagaaaa
     3901 tactatggtg actatcaaaa cctcttacag gacgaagaag tggaagtagt gcatgtctgt
     3961 actcctaatt atcttcacta tcagatgagt aaagatgcgc ttctcgctgg aaaacacgta
     4021 atttgtgaaa agcctttagc tatgaatcgc caggaagcgg aagaattagt agaattggct
     4081 gataagaaag gattagtaaa cgctgttcat ttcaatattc gctactaccc cctggctcgt
     4141 gaggcacacg agatggttaa aggaggagaa gtgggaaaag tattagctgt tcacggttct
     4201 taccttcagg actggctttt ctatgaaact gattataact ggagattaga gccacagttg
     4261 agtggtgatt ctcgggctat agctgacatt ggttcacact ggttagattt ggtagaatat
     4321 gttactcaaa agaaaatagc agaggttttt gctgattttg ccacttttca caaaaccaga
     4381 aagaaacctt taaaaccagt agaaacctat gccggtaaaa tactgcagcc ggaagattat
     4441 caagatgtgc ctatttctac cgaagattat gccagcgttc tgttgcactt tgaagatggg
     4501 gctcatggag tattaaccgt aaatcaggta gcagcaggga ggaaaaatcg ctgttctttt
     4561 gagattgatg gttcacgttc ggctctcttc tgggattcgg aaagacctaa tgagctgtgg
     4621 ataggaagaa gagaccgggc taatgagtta ctcctaaaag acccatctct cgttagtgag
     4681 gaaacgagaa aaattatttc tttcccggga ggacataatg aaggattccc tgatacttct
     4741 aagcagcttt tccgggaaat ttatcagtat attttagaag gaaaaagaga gaattcctct
     4801 ttccccactt ttaaagatgg gcttagagaa gttgttctct gcgaggctat cctccacagt
     4861 gctaaagaag aaaagtgggt taaagttaac taaaagggga gtgagcaaat gaaattaggt
     4921 tttatgactg cttgtctacc agaaatgaag ttagaggaga tagttaagtg ggcaaaagat
     4981 aaaggatttg gtatgttaga ggtggcttgc tggcctcgag tttttgaaaa gagaagatat
     5041 gctggtactc aacacattga tgtagaaaat tttactgaag aaaaagcaca ggaaataaaa
     5101 gaactctttc gcaataatca tttggagata tcatctctcg gctattatcc caataacctg
     5161 gatccggatt tagaaagtcg tcgtttttat catgagcatt tgaagaaagt tatccaagcg
     5221 gcggagaagt taggagtaaa agtggtggga acttttgtag gaagagaccc ccgtatgaat
     5281 gt
//
LOCUS       DAOSVE010000103         5212 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_7070_length_5212_cov_75.276905, whole genome shotgun sequence.
ACCESSION   DAOSVE010000103 DAOSVE010000000
VERSION     DAOSVE010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7070_length_5212_cov_75.276905"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="PK016_07580"
     CDS             <1..455
                     /locus_tag="PK016_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HXL02956.1"
                     /translation="AEEFGESTNSVRLELNRLTQAGLLTSAENGRTKVYQANPNHPLF
                     PEIQSMVRKFTGIDQLIPQVLSQLGEIHSAFVTGDYARGIDSGIIDLVIVGKVDELYL
                     KKLIDKAEQLIHRKIRFLVLSDEELEKLKSTLKIEEAIPLWEEKSSEE"
     gene            630..1574
                     /locus_tag="PK016_07585"
     CDS             630..1574
                     /locus_tag="PK016_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL02957.1"
                     /translation="MGKVVVTGGAGFIGSHLAEELSKRGHKVAILDNLFTGKRENVEP
                     LLKEGKVEFIQGSITDSPLLYKLFQDVSYVFHEAAIPSVPRSVENPQASHEANITGTL
                     NVLLAAKDTGVKKVIYASSSSVYGDTPTLPKKEDMPPNPLSPYAVTKLSGEYYCRVFH
                     RVYGLPTVCLRYFNVYGPRQDPASQYAAVIPRFINRVLENKPPIIFGDGEQTRDFTFV
                     KDVVEANILAAESNAYGVYNIGRGERISINKLAEIIIQLVGNNIEPVYQEARSGDVKH
                     SLADISRARTFGYNPKWSLEEGLRETVNFMRRKVIREE"
     gene            1588..1710
                     /locus_tag="PK016_07590"
     CDS             1588..1710
                     /locus_tag="PK016_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02958.1"
                     /translation="MSEEWEKMGGGVLKPIKDWFSRQGRGKKKESKEREKKKEL"
     gene            1950..2243
                     /locus_tag="PK016_07595"
     CDS             1950..2243
                     /locus_tag="PK016_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02959.1"
                     /translation="MRAEMIEEISQYVALDKNSLLDKGIESLLREKKRGVMLDRLEIL
                     SRYKVSSAEELERKIREGEIEEHPAWEDLIILENLDSALEKINGYLASLSAST"
     gene            2586..2780
                     /locus_tag="PK016_07600"
     CDS             2586..2780
                     /locus_tag="PK016_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02960.1"
                     /translation="MLKVKDLSMDDLEYLIEQKVLEILGDPDSGIELTDEFKKKLAER
                     LEKPSRRISHKEVLEEFGQD"
     gene            2767..3024
                     /locus_tag="PK016_07605"
     CDS             2767..3024
                     /locus_tag="PK016_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015078642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HXL02961.1"
                     /translation="MARIEWTEEAVRDLKVIEKQVGRRILKKLSWFSENFETIVPEPL
                     SGEFKETYKLRVGDWRVIYTIENGVIVIQSIGHRREIYKIQ"
     gene            3197..3411
                     /locus_tag="PK016_07610"
                     /pseudo
     CDS             3197..3411
                     /locus_tag="PK016_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
     gene            3709..4053
                     /locus_tag="PK016_07615"
     CDS             3709..4053
                     /locus_tag="PK016_07615"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HXL02962.1"
                     /translation="MKLDENLKNQLIERLKPLNPEKVILFGSYAYGEPSKDSDIDLIV
                     VTDDDIMPSNFDEKMQIYLKVSNVLTDIQREIPIDLIVHTKPMHKRFIELNSMFYREI
                     AEEGIVIYERNN"
     gene            4037..4438
                     /locus_tag="PK016_07620"
     CDS             4037..4438
                     /locus_tag="PK016_07620"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HXL02963.1"
                     /translation="MKEITEEWLERAYDDIETMGEIIDNPRLTNIVALHSQQAIEKSL
                     KAVIEELEIGAIKTHNLEKLFATVDDFIQFEVDWDLVKKLDALYIDARYPSEWGILSY
                     GKPTMEDARKFYELAMEIYDMVKNFLRSYSE"
     gene            4912..>5212
                     /locus_tag="PK016_07625"
     CDS             4912..>5212
                     /locus_tag="PK016_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02964.1"
                     /translation="MSEDVAHHASRITGNSVVCIIGLGYVGLPLLEAFSQKLKVIGFD
                     INAEKVDKLLSQQPTNSSLATGLSTPDSGLSTNLTFTTDPKEIGKADFIIICVPTP"
BASE COUNT         1632 a          896 c         1277 g         1407 t
ORIGIN      
        1 tggctgagga gtttggggag tctactaact ccgttcgctt agagctcaat cggctcactc
       61 aagctggtct tctcacctcc gcggaaaatg ggaggactaa agtatatcag gccaacccca
      121 accatcctct ttttcctgaa atccagagta tggtgcgcaa attcactggg attgaccagc
      181 tcatccccca ggttctctcc caattagggg agattcattc tgcttttgta actggtgact
      241 atgcccgggg gatagactct ggtatcattg acctggtcat agtagggaaa gtggatgagc
      301 tttacctaaa gaagcttata gataaagcag aacagctcat ccatcgcaaa atccgttttc
      361 tggttttaag tgatgaggag ttagagaagc ttaaaagtac tctgaaaata gaggaagcca
      421 ttcctttgtg ggaggagaaa agcagtgagg agtgagggtg aaagtatctt actgttactc
      481 ccttccgcca ttcccgaaat gcaggcggga atccaccccc gctgtcattc ccgaaatctt
      541 caatcgggaa tccactgaga agaggctttt ccttctcagt atgagtggta gaatagctta
      601 aaattgaaca tccaaagggg aaagaagaaa tgggaaaagt agttgtaacc ggtggagccg
      661 ggtttattgg ctcccattta gctgaggagc tatcaaagcg aggacataaa gtcgctatcc
      721 tggataacct cttcaccggt aagagagaaa acgtagaacc gttacttaaa gaaggaaagg
      781 tagagttcat ccagggtagc attaccgact ctcccctcct ttataagttg ttccaagatg
      841 tgagctatgt ttttcatgag gcagctatcc ccagtgttcc ccggagtgta gaaaaccctc
      901 aggcttctca tgaggcaaat ataaccggga ctttgaatgt acttttggca gctaaagata
      961 ccggagttaa aaaagtgatt tatgcctcat cctcttctgt atatggtgat actccaactc
     1021 tacctaaaaa agaagatatg ccacccaatc ctctctcacc ttatgcagtt accaagctga
     1081 gcggggaata ttattgccgg gtatttcacc gggtttacgg gcttcctacc gtgtgcctgc
     1141 gctatttcaa tgtctatggt ccacggcaag accctgcctc tcaatatgct gctgtcattc
     1201 cccgatttat caacagagtt ctggaaaata aacctcccat aatttttggg gatggcgagc
     1261 aaacccgaga ttttaccttt gttaaagacg tagtagaggc aaatattctg gcagccgaga
     1321 gtaatgctta tggtgtttat aatatcggaa gaggtgagcg tatctccata aacaaactgg
     1381 cggaaataat catccagctg gtgggaaata atattgagcc agtctatcaa gaggcaaggt
     1441 ctggagatgt aaaacacagc ctggcagaca tttcccgagc cagaactttt ggttataatc
     1501 caaagtggag tttagaagaa ggattaagag aaacagttaa ctttatgcgc agaaaagtaa
     1561 tccgtgaaga gtaatgggtg aagggtgatg agtgaagagt gggaaaagat ggggggagga
     1621 gtattaaaac ctataaaaga ttggttctcc cgtcaagggc gagggaagaa gaaggaatca
     1681 aaggagaggg aaaagaaaaa ggagttatga gggggcgttt tgagagaaaa gagtttttta
     1741 tggaggggaa aaatgtcctt attgctggaa gtaggccgat atggaaaaga taaaaatttt
     1801 tgtgataaag aaagtcatgc tatactctcg attaatgact attgactatt gactgccgac
     1861 tgttgttaag actctcgatt aatgactcct gactatgaat agccgtgata taataacatc
     1921 ggatgcaaga ttttacgagg aggatagcaa tgagggctga gatgattgag gagataagtc
     1981 agtatgtagc tctggacaaa aactccttgc tggacaaagg tatcgagtca cttttaaggg
     2041 aaaagaagag gggagttatg cttgaccgac tggaaatact ttctcggtat aaggtttcct
     2101 cagcagagga attagaaaga aaaattcggg agggagaaat agaggagcat ccagcctggg
     2161 aagacttaat aatcctggaa aatcttgatt ctgcactgga aaaaataaat ggttatcttg
     2221 caagtttatc agcgtcaact tgaagtagca aggcaaaaat ttggtgacat cgtaagtgaa
     2281 ggtgaaatcc tttatacaga aagcaaagag ccattaaagt taaggctaag tatagtggat
     2341 ggctcaattg tagacgtttt ttactcttct agaggtaaat actcctatca ttgggataga
     2401 ccctcatgcc ttatgaacaa ggtataaggg ccataataga tgatgctaag gctcgaaaga
     2461 tggcagaaaa gcagtgatgc gtgaggagtg gggattaatc agtaatgagt aatgagtgat
     2521 gcgtgagtgg tgagggtgag cggttcgcaa cttgacctaa aagggttgac taaaaggagg
     2581 cagagatgct aaaagttaag gatttaagta tggatgactt ggaatacttg atagagcaaa
     2641 aggttttaga aatactcgga gatcctgatt ccgggataga attaacagat gagttcaaga
     2701 agaaattagc agaacgtctg gagaaaccgt ccagaagaat ttctcataaa gaggtattag
     2761 aggaatttgg ccaggattga atggacggaa gaagcggtaa gggatctcaa ggtaatcgaa
     2821 aagcaagtag ggcgaaggat tcttaagaaa ctcagctggt tttctgagaa ttttgaaaca
     2881 attgttcctg agccattaag tggagagttt aaagaaactt acaaattacg ggtaggtgat
     2941 tggcgggtta tatatacaat tgaaaacgga gtcatagtaa tccaaagtat tggtcatcga
     3001 agagaaatct acaagataca gtaatcagtg atgtgtgagg gtgaaagtga aagtgaatgt
     3061 aaaaaatgat agaaagtaat aacgtatcac acctctcatc ttgcagcatt tatcacatat
     3121 tatatggtat tatttggttg gaggtgatgt tgtgtcaaag ctagagctgg agatacctga
     3181 ggagctggtt tagtcactga agattccaag agatgaagtt ccggcgagac tgaaaagaga
     3241 gttagctctg cggctctatg agaagggctc gcttagcttt ggaaaagccc gtgaacttgc
     3301 tgggatgacc aagtgggagt tttcctatct tttggggaag aagagattcc tcgccactat
     3361 gatcttgaag agtttaacaa agacctcaat accctggaaa agctggattg actgtgatct
     3421 gtaattcttc gatattgatt gccttgagtg caattggtcg gctggatctg ttgagaaaac
     3481 gatgtaagga tgcgaaacta atagtccccg ctgcagtatg gaaagaaata gtatagatta
     3541 tggagaggct gaagtcatag ccttgacgct ggagaaaaag acggatttag ttctgcttga
     3601 cgagaaagag gcaagagaag ttgggtttcc ggattttggg aacggtgggt ttactcatag
     3661 gggcaaaacg agaggaatta tattatttaa agtgatatac taattattat gaaacttgat
     3721 gaaaacttaa aaaatcaatt gatagagagg ctgaaacctc taaatcctga aaaggtaatc
     3781 ctttttggta gttacgccta tggagagcca tcaaaagata gtgatattga tttgattgtg
     3841 gtaaccgatg atgatattat gccaagtaat ttcgatgaaa aaatgcagat ttacttgaag
     3901 gtctctaatg tacttacaga tatacaaaga gagatcccta ttgaccttat cgtgcacaca
     3961 aaaccgatgc ataaaagatt tatagagctt aatagtatgt tttaccgcga aatagctgag
     4021 gagggaatcg taatatatga aagaaataac tgaggaatgg ctcgaaaggg catatgatga
     4081 tattgagact atgggtgaga tcatagataa tcctcgcctg acaaatattg tggctcttca
     4141 ctctcagcaa gctatagaga aatctctgaa ggcagttata gaggagctcg aaataggagc
     4201 tatcaaaact cataatttgg aaaaattgtt tgctaccgta gatgatttta tacaatttga
     4261 agttgattgg gatttagtta agaaacttga tgcgctatat atcgatgctc gctatccatc
     4321 agaatgggga atattgtcat atggaaaacc gaccatggaa gatgcaagaa aattttacga
     4381 attggcaatg gaaatatacg atatggtcaa gaattttttg aggtcatatt cagaatgaga
     4441 aataacctgc aaaacagcat ttaccaatgc ttgatttctt tcgctggagt gtttatctcc
     4501 agcgtaatta gttttcttca cctatttttg ctttcgtctt tagcaccgca ggcacgctgg
     4561 atgcttttcc cagcgttgtt tttccagatt cactggtgga gcttttccca ccagtggtct
     4621 ttgcttttta tcttttattt ttttgccctt tccttttcct cctcccgccc ctggagggag
     4681 ggagttagag ggagggggaa aattgctgaa gaaatatgat tttgagggaa agacaccctc
     4741 accttagtcc tcttcccgtc aagggagagg gaaagaaaaa agaaagaaag gggttataag
     4801 ggggtctttc cccctttata gttattgtaa tggattttgc ggttaactat aatgtcagaa
     4861 aaatgtttct cagattaatc aaaaatttaa aaaacaagca ggtggacgca aatgtctgaa
     4921 gatgttgctc atcatgcatc acgcataact ggtaactcag ttgtttgtat catcggtctg
     4981 ggatatgtgg gcttgccgct tcttgaggct ttctcccaga aactcaaagt aattggcttt
     5041 gatatcaatg ctgagaaggt agataaatta ctcagccagc agccgacaaa tagctccctc
     5101 gccactggac tctcgacccc agactccgga ctctcgacta acttgacttt taccaccgac
     5161 cccaaagaaa taggcaaagc tgattttatc atcatctgcg tccccacccc gg
//
LOCUS       DAOSVE010000104         5015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_7370_length_5015_cov_69.086492, whole genome shotgun sequence.
ACCESSION   DAOSVE010000104 DAOSVE010000000
VERSION     DAOSVE010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7370_length_5015_cov_69.086492"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(221..1264)
                     /locus_tag="PK016_07630"
     CDS             complement(221..1264)
                     /locus_tag="PK016_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HXL02965.1"
                     /translation="MAQTMKAAVMKGIGQIEIEELPIPIPREEEVLVRIKSVGVCGSD
                     VHYFVEGRIGDYVVKPPFILGHECAGEVVEIGKKVKNLKPGDRVTMEPGVPCGKCEFC
                     RSGRYNLCPDVVFWATPPVNGTFCEYVTHPANFTYLIGPNVSFEEGALVEPLAVGVYA
                     THRAYVEPGEVALVLGSGPIGLVTIEALSARGVTRIIAVDVFDFRLKKAEEVGAKFTI
                     NAQKEDIKSRVKELTQGRGVDVVFETAGGIPTSQLTVDVTRKGGRVVLIGLPSQTHFD
                     FELLKVVDKELDILGVFRYANAYLGCVDLLNAGKVNLQSLITHRFTLEETQKALQFAH
                     EHKEESIKVVVNL"
     gene            complement(1279..1920)
                     /locus_tag="PK016_07635"
     CDS             complement(1279..1920)
                     /locus_tag="PK016_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HXL02966.1"
                     /translation="MFLQDKDKETLVNFLKENLSRKVSLKFFTQELECETCHSVRSLL
                     EEVVALSPLLHLETYNFLLDKDKANLYGIDKIPAIVVEGEKDYGIRFYGIPAGYEFSG
                     FIETLIWVSRGQTDLKKESKDFLSSLTREVNIQTFVTVTCPYCPQAVVLGHKMAIEGE
                     KVRSAMIEVEEFPHLAYRYGVRAVPKIVINEQMSFEGAVPEEYFLTKIMEAVS"
     gene            complement(1932..2900)
                     /locus_tag="PK016_07640"
     CDS             complement(1932..2900)
                     /locus_tag="PK016_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI/YhbO family deglycase/protease"
                     /protein_id="HXL02967.1"
                     /translation="MAVRRIIVLVESQYQELELWYPLLRLREAGIEAKLVGVNVEETF
                     YGRHGFPARAEMAISLVNPRDFDGVIIPGGFAPDYLRRLPVVLNLVREFYQGGKLVGA
                     ISRGPWVLVSADIVRGKRIAGLVSIKDDVNNAGGDFTDLPVMVDGNIITAQGPDDLPF
                     FMEEALYFLWQNKPCLNSPSPDGWLTDTTGNSVLLSQLLQGKAGVILFFDSVFSPQGS
                     QTLPHYLSLKEEIEKKKGTLIPISSDTFFAQREFIERNNLPFPLWSDPGAQLIKAFGV
                     WRKDSLARWAVFLVDRQGFLRYGEDCPGGDLDNLPGFQRKLESILG"
     gene            complement(2904..3398)
                     /locus_tag="PK016_07645"
     CDS             complement(2904..3398)
                     /locus_tag="PK016_07645"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HXL02968.1"
                     /translation="MVLRFSPSEILETAKELERRGIDFYCQLAQLTSSPSAREVFSFL
                     GEEEKKHLDFFTNLQKELDEGVEIVFSTTDESINYLGAIVESGILDQVLRGMNLTPVG
                     IKMEDALEIGIEIEKESILFYQGLIPMVSPEKREWLDKVIKEEKKHFLRLQSMKRELK
                     EKEG"
     gene            complement(3583..4554)
                     /locus_tag="PK016_07650"
     CDS             complement(3583..4554)
                     /locus_tag="PK016_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HXL02969.1"
                     /translation="MAVHFTELGLVNTREMFKKALAGKYAVPGYNFNNMEQLQAIITA
                     CVECNSPVILQISKGARQYANQTLLRYMVPGAVQMAREMGSNIPIALNLDHGDSFELA
                     KSCVDYGFSNVMIDGSHLPYEENVAITRKVVEYAHAYDVTVEGELGVLAGIEEDVSSS
                     VSHYTDPAQVEDFVGRTGVDSLAISIGTSHGAYKFKLKPGEPFPTLRFDILEEVERRL
                     PGFPIVLHGASSVLKKYVDIINQYGGALEDAVGVPEDQVHRAAQSAVCKVNIDSDGRL
                     VMTAMIRKYLAENPKEFDPRKYLGYAREELKKMYIAKCELLGSAGQA"
     gene            4941..5013
                     /locus_tag="PK016_07655"
     tRNA            4941..5013
                     /locus_tag="PK016_07655"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4974..4976,aa:Thr,seq:tgt)
BASE COUNT         1402 a         1196 c          943 g         1474 t
ORIGIN      
        1 gcaaaacctg ttataatttc tctccgaaaa gattgctaca ccaagcaagc aaacgaagct
       61 cgcaacgacg gtttagtagg gattatcaca taattattct ttgctatact gcagaaaagg
      121 cggagtgtga actaagaaaa cattattgtg agttttatga ttaactataa tgtcgaaaaa
      181 taaagggggg tttaaagtaa accccccaag gtcaaatttt tcacagattc actactactt
      241 tgattgattc ttccttgtgt tcgtgggcaa actgtagagc tttttgagtt tcttctaagg
      301 taaaacgatg agtaatcaaa gattggaggt ttacttttcc agcattcaat aaatctacgc
      361 aaccaaggta ggcattagcg taacggaaaa cccccaggat atccagttct ttatccacca
      421 cttttaaaag ctcaaaatca aaatgagttt gagaaggaag accgatgagg accactctcc
      481 ctccttttct ggtgacatcc acagtgagtt gagaagtggg aatacctcct gcagtctcaa
      541 agactacatc tactccccga ccctgagtta gctcttttac tcgagacttt atatcctctt
      601 tttgagcatt tatcgtgaat tttgctccca cctcttctgc tttcttcaag cggaagtcaa
      661 aaacatccac tgctatgatt cgggtaactc ctcgagctga aagagcttcg atagttacaa
      721 gtcctatagg gccgctacct aaaaccagag ctacctctcc tggctctaca tatgcccggt
      781 gagtggcata tactcctaca gctaggggtt caaccagtgc tccttcttca aaactcacgt
      841 ttggtcctat gaggtaagta aaattggccg ggtgagttac atattcgcaa aaagtcccat
      901 tcaccggggg agtagcccag aaaactacat ccgggcagag attatatcta ccagagcgac
      961 agaactcgca ctttccgcag ggaacaccag gctccatagt aacccggtct ccgggtttta
     1021 aatttttgac tttctttcct atctccacta cttctccggc gcactcatgg cccagaataa
     1081 aaggaggctt caccacgtag tcgccgattc tcccctccac aaagtaatga acgtcagagc
     1141 cacatactcc tacgcttttt atccgtacca gtacctcttc ttctctgggg ataggaatag
     1201 gcaactcttc aatctctatt tgcccgattc ctttcataac tgctgctttc atggtttgag
     1261 ccatataatt tcctcctttc aagacactgc ttccataatt ttagtcaaga aatactcttc
     1321 cgggactgct ccttcaaaag acatctgttc attgattact attttaggca ctgctctcac
     1381 tccgtagcga taagccagat ggggaaattc ttctacctct atcattgccg aacgtacttt
     1441 ttctccctct atagccattt tgtgtcccag aaccactgcc tgcgggcaat agggacaggt
     1501 aacggtcaca aaagtctgga tattaacttc tctggttagg gagctcaaaa aatccttact
     1561 ctctttcttt aaatcggttt gcccccggga aacccatatc agggtctcta taaaaccaga
     1621 aaattcatag ccagctggaa ttccataaaa gcggatacca taatcttttt ctccttccac
     1681 tactatggct ggtattttat caatgccata aagatttgct ttatccttat ctaaaagaaa
     1741 gttataagtc tcgagatgga gaagaggaga aagagctact acctcttcta agagtgagcg
     1801 tacactgtgg caggtttcac actctaattc ctgagtaaaa aacttcagac tcaccttacg
     1861 ggataagttt tccttcagaa agttaaccag agtttcttta tccttatcct gtaaaaacaa
     1921 atttaccacc cctatcctaa gatactttcc agttttcttt ggaaaccagg gagattatct
     1981 agatccccac cgggacaatc ttcaccataa cgcaaaaacc cctggcggtc taccaaaaag
     2041 actgcccacc gagccagaga atccttcctc cacaccccaa aagctttaat caattgagca
     2101 ccagggtcac tccatagagg gaaagggagg ttatttcttt ctatgaattc tcgttgggca
     2161 aaaaaggtat ctgagcttat aggtattaaa gtgcccttct ttttttctat ctcttctttt
     2221 agagaaaggt agtgggggag tgtctgactt ccctgagggc tgaaaaccga atcaaaaaag
     2281 aggattactc ctgctttacc ttggaggagc tgggaaagaa gaactgaatt accggtagta
     2341 tcagttaacc agccatcagg agaaggacta ttaagacaag gtttgttttg ccacagaaaa
     2401 tacagggctt cttccataaa gaaaggtaag tcatcaggac cctgggcagt gataatattt
     2461 ccatctacca taactggtaa gtcggtaaaa tctcctcctg cgttattcac gtcatcttta
     2521 atggatacaa gtcctgctat cctttttcct cttacaatat cggcagagac cagcacccaa
     2581 gggccccgcg aaatagcccc tactaatttt cctccctgat agaattctct gactaaattg
     2641 agcaccactg gaagacgacg caaataatcg ggagcgaaac caccaggtat aatcactccg
     2701 tcaaagtctc gaggattaac taaagagata gccatttctg ctcgcgccgg aaaaccatga
     2761 cggccataaa aagtctcttc tacatttacc cctactaatt ttgcttctat tcctgcttct
     2821 cgcagacgga gcaggggata ccatagctcc aattcttggt attgactttc taccaaaact
     2881 attatccggc gcacagccat tttttatccc tccttttctt ttagctctcg cttcatactt
     2941 tgaagacgca aaaaatgttt tttctcctct ttaataactt tatctaacca ttctcttttc
     3001 tctggagaaa ccatgggaat aagtccttga tagaaaagga tgctttcttt ttctatttct
     3061 atacctattt ctaaagcatc ttccatcttt atccctacag gggtaaggtt cattcctctt
     3121 agcacttggt ccagaattcc actttctaca attgctccca gatagttgat actctcatca
     3181 gtagtagaaa agacaatttc tacgccctca tcaagctctt tctgcaggtt agtaaaaaaa
     3241 tccagatgtt ttttttcttc ctctcctaag aaggaaaaaa cctctcgggc agaaggagaa
     3301 gaagttaatt gtgccagctg acaataaaaa tctatacccc ttctttctaa ctctttagcc
     3361 gtttccagga tttcagaagg agaaaaacgt aataccattt ctatcacatc ccttcctatc
     3421 tttagctgta gtatgaggga gatatctccc tcatacttac tgcctcgtta ctggtaatcg
     3481 cttgatgtgc gatgcttttg acttgattta aattctatcc tgtggggatg ccatccctta
     3541 aactgcaggc acccgaagat accttgctgg tccaggattt tatcaggcct ggcctgcgct
     3601 acctaagagc tcgcacttag cgatatacat tttcttgagt tcttcccgag cataacctaa
     3661 atacttgcga gggtcaaact ccttgggatt ttcagctaaa tattttctaa tcatagctgt
     3721 cattaccagc cgcccatcgg aatcgatatt gactttgcat accgctgatt gagcagcacg
     3781 atgaacctgg tcttcaggta caccaacggc atcctctagt gctcctccgt actgattaat
     3841 aatatctaca tatttcttca atactgaaga agctccatgg agcacaatgg ggaaaccagg
     3901 gagccgacgt tctacctctt ccagaatgtc aaaacgaagg gtggggaagg gttcaccggg
     3961 ttttagctta aacttatagg ctccatgaga agtaccgatg gaaatggcta agctatctac
     4021 ccccgtccgg cctacaaaat cctctacttg tgcagggtca gtgtagtgag aaacgcttga
     4081 agaaacgtct tcttcaattc cagccaggac acccagttct ccttctactg ttacgtcata
     4141 ggcatgagca tattccacta cttttctggt tatggctaca ttttcttcat agggaagatg
     4201 agagccatca atcatcacat tagaaaatcc gtagtccaca caggatttcg ctaactcaaa
     4261 agaatctcca tggtctaagt ttaaagcaat ggggatgtta cttcccattt ctcgagccat
     4321 ttgtactgct ccaggaacca tatagcgcaa cagggtttgg ttagcatact gacgggcacc
     4381 cttggaaatt tgcaaaatta ccggtgaatt gcattccaca caggcagtaa taatagcctg
     4441 cagctgctcc atgttattaa aattgtaacc tggcacagca tattttccgg ccagagcctt
     4501 tttaaacatt tcccgagtat tgaccaatcc cagctcagta aaatgaacag ccattattat
     4561 ttcctccctc tttcgattta tgttatcatt gttgtaaaca attctctctt attttacaac
     4621 tttcttcaag tagtgtgtaa ttttacacta acataaaatg caggagaaat actgaaaagg
     4681 attatggtga taaccatgtg ggagagcaac ttatttctgt cgttgagaac ctcacttgtt
     4741 ccttgtcgaa cagtcgcgtt tcttaaggga ggggttgttc tgcctcccca caccttgcag
     4801 agacgaaaaa aagaggttta taaagagcgc ctccctttgt agttttgaca ttatattaga
     4861 ataaatataa atggagcttc gtttctcttg acattcttct tacctttgag tatatttatt
     4921 gagacacggg cgataaggtc gccggtgtag ctcagctggt agagcagctg acttgtaatc
     4981 agcaggtcgt ccgttcgagt cggatcaccg gctcc
//
LOCUS       DAOSVE010000105         4937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_7489_length_4937_cov_66.412331, whole genome shotgun sequence.
ACCESSION   DAOSVE010000105 DAOSVE010000000
VERSION     DAOSVE010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7489_length_4937_cov_66.412331"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PK016_07660"
     CDS             complement(<1..210)
                     /locus_tag="PK016_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HXL02970.1"
                     /translation="MEQIYHIRFEHNEKGKSLRRIAQETGFNFRTVRKYVEREDFNIK
                     KKAIHTRRGKLDPYKETIDKWLTEDL"
     gene            450..1178
                     /locus_tag="PK016_07665"
     CDS             450..1178
                     /locus_tag="PK016_07665"
                     /inference="COORDINATES: protein motif:HMM:NF015549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HXL02971.1"
                     /translation="MEENIEHSIKQKEVPSKFREGMVASLPIFIGYIPAAVTFGLLAK
                     STGLTLLETFLFSALVFAGASQFMALQLIQGGAGPAQIVVATLIMNFRHFLMSSHLST
                     IFPIKEKPWLLPFLSFGVTDESFAYFSTSHTPMGVDFILGLEYSAYAGWVGGTVVGYL
                     FGNILPPIFQASMGIALYALFVALLIPEAKKSFSITIVAIIGGLTHSFLTWLKFFSPG
                     WNIILAILIAASAGAWIVDEEENQ"
     gene            1175..1483
                     /locus_tag="PK016_07670"
     CDS             1175..1483
                     /locus_tag="PK016_07670"
                     /inference="COORDINATES: protein motif:HMM:NF017273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HXL02972.1"
                     /translation="MNNIKIIIGMMLVTFLPRYLPFLAFSRLNIPRPLKKLLQYITPA
                     ALGALILPGAITAIPDYPLAATAGIVTAIACSWFRGEISLTVFLSVIATLVGLYFSGY
                     "
     gene            complement(1754..2587)
                     /locus_tag="PK016_07675"
     CDS             complement(1754..2587)
                     /locus_tag="PK016_07675"
                     /inference="COORDINATES: protein motif:HMM:NF016952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HXL02973.1"
                     /translation="MKNFVLSYKATCKILGVTVWLFVIFVRLDAWCYDSLALHLSRNE
                     DIPHYPAQVVAIYPHDPQAFTEGLVYEDGFLYESTGLYGESSLRKIDLTTGEIIHLVE
                     VEGRYFAEGLALVDSNLIQLTWKSKVVFLYCKDTLELEKTLYYPYEGWGITYDGNCFI
                     VSDGSHLLRFLDPVNFSLMKELPVKLGNRPIDQLNELEYINGKIYANVWHTDIIIIID
                     PQSGEVVGCIDLASLEEKRNTGEDVPNGIAYDAENNRLFVTGKRWSHIYEIQLPKINE
                     N"
     gene            2709..3737
                     /locus_tag="PK016_07680"
     CDS             2709..3737
                     /locus_tag="PK016_07680"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HXL02974.1"
                     /translation="MDYTYQEILKQSTTWEKTLHYLKEQENYFSQFLAETYDNVIFTG
                     CGSSYNLSLVASHFWQTGKQIPARGVPASETFLFPGGVFLPRNRYLLFGISRSGETTE
                     TVKALQHFQDKFKGKTVGVSCEENSSLLKAADYQISLPFAHEESVVMTQSFPSILLSF
                     LFLSLLESKSTDNLSLLPEILEKYLSSGEKTIKDLVSNLSFDKFIFLGNGPYYGIAWE
                     GSLKLKEMSLTSTETFHFLEFRHGPKSVVDEKTLIIALSSQRAFSWEKEVMMEMMDLG
                     ATILHLGKNPLNSPRAIEITFSEEILDDFSLPVLYLPFLQLLGYYRAKNKGLNPDTPR
                     YLAKVVKI"
     gene            3734..4879
                     /gene="nagA"
                     /locus_tag="PK016_07685"
     CDS             3734..4879
                     /gene="nagA"
                     /locus_tag="PK016_07685"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HXL02975.1"
                     /translation="MRWGIKGKIVTPQEVIPGLVIVRENRIEDVLSHPGGEDLEVVYD
                     FEDNFIFPGLVDLHLHGLQGEDVTEGGVKSIQKISTLLPFSGVTSFLPTTLTTDLATL
                     LKIVEETKAWGGSSSSGARMLGLHLEGPYLSSGRRGAHNSTYLREPHQEEIKSLLQKG
                     EGIIKRITIAPELPGALETIEYLAQHNILVSLGHSEANYETSRQAWKRGAKMVTHLFN
                     GMDPLHHRQPNLLAFALGNDEIYAEIIADKIHVRPEIMKIALKCKAPDHLCIVSDALK
                     VTNLPEGVYELSGQKVEVREGMAYLLSGTLAGSTFLLNQMLYNLKELFSIPLPQLAEM
                     GSFIPAQLVGKEKELGSIEKDKIADIVIFDDHFQVKTTFINGIRVKG"
BASE COUNT         1474 a         1003 c          914 g         1546 t
ORIGIN      
        1 taggtcttca gtaagccact tatctatggt ttctttatag ggatccagtt tacccctacg
       61 ggtatgaata gctttcttct tgatgttgaa atcctccctt tctacatact tccgaaccgt
      121 tcggaagtta aatcctgttt cttgggctat ccttctcaat gatttacctt tttcattatg
      181 ttcaaatctg atatgatata tttgctccat tgttagcatc cctttcttta ccccctttag
      241 attcagcatc taaaagggta gtttatttta agggtgctgg caatggagtt tctatttcct
      301 gctgtatttt ttaacttcca ctgtgctaca tttctattct accaaacaca tctgcgtaaa
      361 aacaaaaaac ataatcctac agcactacta ctattttttt tgggatataa tatcaggaaa
      421 aatagaaagt taatggtgag gaggttaaca tggaagaaaa catagaacac tctataaaac
      481 aaaaagaagt accttcaaaa tttagagaag gaatggttgc ttccttgcca atctttattg
      541 gatatatacc ggccgcggtg actttcggac tattagcaaa atcaacagga ttaactttgc
      601 tagaaacatt tcttttttct gccttagtat ttgccggtgc cagccagttt atggctttac
      661 aactcatcca gggaggggca ggaccggctc aaatcgttgt agccacttta attatgaact
      721 ttcgccactt ccttatgagt tcacacctat ccactatttt ccctataaaa gaaaagccct
      781 ggcttcttcc ctttttgagc tttggtgtaa ctgatgaatc cttcgcgtat ttctccacct
      841 ctcatactcc tatgggagtc gattttattt taggccttga atattcagct tatgctggct
      901 gggtaggagg aaccgtagtg ggttatttat ttggtaatat tttacctcct atctttcagg
      961 ccagtatggg aatagcactt tatgctcttt tcgttgctct actcatccct gaagctaaaa
     1021 aatctttttc gataacaata gtcgctataa taggggggct gacgcattct tttcttacct
     1081 ggcttaaatt tttttctccg ggctggaata taattctggc cattttaata gctgctagtg
     1141 ctggagcctg gatagtagat gaggaggaaa atcaatgaat aacattaaaa taattatagg
     1201 gatgatgctg gtcacttttc ttcctcgtta tcttcctttt ctggcttttt ctcggctcaa
     1261 tattccccga ccgcttaaaa aacttttaca atatatcact cccgctgctt taggagcatt
     1321 aatacttccc ggggcaataa cggctatccc cgattacccc cttgcagcta ctgcaggtat
     1381 cgttacagca atagcctgtt cttggttcag aggagaaatt tccctgactg tatttttatc
     1441 agtaatagct accttagtcg gtttatactt ctccggctat tgagtttgca gtcactgcaa
     1501 actactatgt tggtaatata aggtcctctc ttccatggct ttttgtcatt ctgaaattca
     1561 gccgggaatc catatgcttc caaggtagtg ttttttctct cccggtagta tctgtttttt
     1621 attatatttg ttgttttctt aaagggggta ataaggggct atttcccctt ttaatcggtt
     1681 ataaagagat tcccacttat tgctaataat agattttgcg agatactact ataccattat
     1741 gtagtagggt tacctaattt tcattaattt tcgggagttg tatctcataa atgtgagacc
     1801 atctcttacc agtcacaaac aagcggttat tttcagcatc gtaagcaatt ccatttggga
     1861 cgtcttctcc agtatttctt ttttcctcga gtgaggcaag gtcaatacac cctaccactt
     1921 caccactctg aggatcgata attataataa tatcagtatg ccagacattg gcataaatct
     1981 taccgttaat gtattctaac tcattaagct ggtcaatggg tctatttccc agtttgacag
     2041 gtaattcttt cattaaagaa aaatttacag ggtcaagaaa ccgcaacaaa tgggaaccat
     2101 cacttacaat aaaacaatta ccatcatagg taatacccca cccttcataa ggataataga
     2161 gggttttttc gagttccaaa gtatctttac agtaaagaaa gacaactttc gatttccagg
     2221 tcaattgaat caaattagaa tccacaagag caagaccttc tgcaaaatac cggccttcaa
     2281 cttctaccaa atggataatt tctccagtag taaggtcaat cttccttaaa cttgactcac
     2341 catataagcc agtactttcg tataaaaaac cgtcttcata gaccaatccc tcagtaaaag
     2401 cttgaggatc atgaggataa atagctacca cttgagccgg gtaatgagga atatcttcgt
     2461 tacgggagag atgcaaagca agcgagtcat aacaccaggc atccaatctc acaaaaataa
     2521 caaagagcca cacagtaact cccagtattt tacaagtagc tttataagaa agaacaaagt
     2581 tcttcatacc ttttatatca tatcataatt tttgttttga gctaaagcct tttgtagcta
     2641 agccagatac caacaaataa aatgaggtat aatggtttta aataagaaat ttcaaaggag
     2701 gaatgcttgt ggattacacc tatcaggaaa tactcaagca atctactacc tgggaaaaga
     2761 ccctgcatta tctaaaagag caagaaaatt atttctctca atttttagca gaaacatacg
     2821 ataatgtcat tttcactgga tgtggaagct cctataacct ctctcttgtt gccagccatt
     2881 tctggcaaac agggaaacaa attcctgcca gaggagtgcc tgcttctgaa acttttctct
     2941 ttcctggagg ggtattttta ccccggaacc gataccttct ttttggtatt tctcgctcag
     3001 gagaaactac agaaacagtc aaagctcttc agcacttcca ggataaattt aaagggaaaa
     3061 ctgtaggagt aagctgcgaa gaaaattcct ctcttctaaa agcagctgat tatcaaatat
     3121 ctctcccttt tgctcacgaa gaaagcgtgg taatgactca atcttttccc agtatattgt
     3181 tatccttcct cttcttgtct ctcctagaga gcaaaagtac tgataattta tcccttctcc
     3241 ctgaaatttt ggaaaaatat ctttcatctg gagaaaaaac tataaaagat ttagtttcta
     3301 atctttcttt tgataaattt atatttttag gtaatggccc ttactacggt atcgcctggg
     3361 aaggctccct aaagctcaaa gaaatgagtc taacttctac tgagaccttc cattttctgg
     3421 aatttcgcca tggtcctaaa tcagtcgtag atgagaaaac tttaatcatt gctctatctt
     3481 cacagagagc tttttcctgg gaaaaagaag taatgatgga gatgatggac ttaggagcca
     3541 ccattcttca tctaggaaaa aatcctctta actctccccg agcaatagaa ataacatttt
     3601 cagaagagat tttagacgat ttttccctcc cggtgctata tttacctttc ttgcaacttc
     3661 tgggctatta tcgagcaaag aataaggggt taaatcctga taccccaaga tacctggcta
     3721 aagtggtaaa aatatgaggt ggggaattaa gggtaaaatc gttactcctc aagaagtaat
     3781 tcctggcttg gtgatagtga gagaaaatcg catcgaggac gttctatccc atccgggggg
     3841 tgaagatttg gaagtagttt atgactttga agataatttc atattccccg gccttgtgga
     3901 tttacacctc cacggtcttc agggagagga tgttactgaa ggtggggtaa agagtattca
     3961 gaaaatcagc actctcctgc ccttttccgg ggttacttct tttcttccta ccaccttaac
     4021 tactgattta gcaactcttc ttaaaattgt tgaggaaact aaagcctggg gagggagttc
     4081 ctcttcagga gctcgaatgc tgggccttca cttagaaggc ccttatttat cgtcaggaag
     4141 aagaggagct cataattcca cctacttaag ggagccccac caagaagaga taaaatcctt
     4201 acttcaaaaa ggagaaggaa taattaaaag aataaccata gctcctgagc ttcccggagc
     4261 actggaaacc attgaatatt tagctcagca taatattttg gtttctttag gacacagtga
     4321 ggcaaattac gagacctccc ggcaagcctg gaaaagagga gctaaaatgg taactcacct
     4381 ttttaatggt atggatcctc ttcatcaccg ccaacccaat cttttagctt ttgccctggg
     4441 gaatgacgaa atatatgctg agattattgc tgacaaaatc catgtaaggc cagaaataat
     4501 gaaaatagct ctgaaatgta aagctcccga ccatctttgt atagtaagtg atgctctgaa
     4561 agtaaccaac ttaccagaag gagtatatga attgagtgga cagaaagtag aggtaagaga
     4621 aggaatggct tacctacttt caggaacttt agctggtagc accttccttc ttaaccaaat
     4681 gctttataat ttgaaagagt tattctctat ccccctaccc cagttggccg agatgggaag
     4741 ttttatccct gctcagctag taggaaaaga aaaagagttg ggtagcatcg agaaagataa
     4801 aatagctgac attgtaatat ttgatgacca ctttcaagta aaaactacat ttataaacgg
     4861 aattagagta aaggggtaag caattatgta taaattgagt caggtttgga ggtaaaaaca
     4921 ccgtagaaga attcctc
//
LOCUS       DAOSVE010000106         4897 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_7572_length_4897_cov_72.800083, whole genome shotgun sequence.
ACCESSION   DAOSVE010000106 DAOSVE010000000
VERSION     DAOSVE010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7572_length_4897_cov_72.800083"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(469..1116)
                     /locus_tag="PK016_07690"
     CDS             complement(469..1116)
                     /locus_tag="PK016_07690"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HXL02976.1"
                     /translation="MDYKAFASFSGGKDCHLSILRAQETGWEVRFLFTMLQEDGAFSR
                     AHHLPSFILERQAQAMGLELQKANATWEEYEDNFKETIRFLKGKGINSGVFGDIDLAE
                     HREWVERVCKEEGIYPLLPLWGEEHQKVAQEIVKRGFVAFVIMVNDQFLDSRYLGRRY
                     DYSLIEELVNLGIDPCGEKGEFHTLVVDGPLYHYPIPYQWGEIKEEDGHHFIEVK"
     gene            1523..2686
                     /gene="dnaJ"
                     /locus_tag="PK016_07695"
     CDS             1523..2686
                     /gene="dnaJ"
                     /locus_tag="PK016_07695"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HXL02977.1"
                     /translation="MKRDYYEVLGVNRNATSEDIKRAYRRLARQYHPDVNQGDNGAAE
                     KFKEINEAYQVLSDEQKRATYDRFGHSAFEGANQGSYGGFGSDFGPFGDFSNFGDIFD
                     FFFGGGTQSRREHRPRAAKGPDIKVEITLGFEEAAQGVEKEISFSRTEICPECQGIGG
                     KRKVACPHCQGRGEVRHTQTSLFGSVVTSRPCSYCQGKGWVAEDICSLCRGSGKIKNE
                     RKIKIKIPAGVDSGYRLRVAGEGEAGENGGPSGDLYAYIKVKPHPVLERKGSDLYYDL
                     ELSYPQLVLGDEVEIPTLRGKETIRIPAGTESGALLRLRGKGLPDPQIGSRGDQIIRV
                     KLRVPRRLTLEHRQALEKLMELERVENSLEGNGDNRKQSGVFERLKEAFTRPE"
     gene            2698..3423
                     /locus_tag="PK016_07700"
     CDS             2698..3423
                     /locus_tag="PK016_07700"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HXL02978.1"
                     /translation="MSRFLFHSPLVIGETILLSPRESHHLLVERIKPGSSILLSDGKG
                     NLFRGTYVEEKRGRARIRVEEKVEEEKRSFNLTVWQAILKSPSRMDWIIEKLTEIGVS
                     QIGFFSSHRSLKNVISPEKMERWEKIALSACKQSGRTIFPDLVFLEQWEGFVSHLSSY
                     PGVTILADPEGDETLWSFLQGKKERNFQIVIGPEGDLTEEEKEEILEKRPGKRIKLSK
                     KILRSETASLFSASVVTSFLEDK"
     gene            3420..4673
                     /gene="mtaB"
                     /locus_tag="PK016_07705"
     CDS             3420..4673
                     /gene="mtaB"
                     /locus_tag="PK016_07705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HXL02979.1"
                     /translation="MKVAIKTLGCKVNQVESDILATTFSQEGVEVVSFGEEADLYIVN
                     SCAVTKEAENKTRQLVNRILRKHLAPRIILTGCYAYRISRERENPFEESVFLLPDFPK
                     EKEIINLVGKWWGEKFTLAEYPLPSRARAWLKVEDGCDHFCSYCLIPHLRGKIKSEEK
                     EKIIRQAQALEREGIREIVLCGVNLGYYGKDRGEKGLINLLEELVDNTTLVRFRLSSI
                     EPFLLDEEFVKRYFSLRDRVCPHFHLPLQSGSDRVLKSMRRGYDTTRYRALVSLLRSY
                     CPAVAISTDIIVGFPTEEKEDFENTVNFSQEIGFSRTHVFVFSPRPGTPAFLWEKERG
                     TPKKEKKNREKILLQVARETQKKYYHLFLGKKLKVLVESIQDGTSSGYSENYIPVRFR
                     GNSKEREIVEVTIEKEGDGFLEGKL"
BASE COUNT         1536 a          955 c         1082 g         1324 t
ORIGIN      
        1 gttgtatttg ctctctttat tttgtctttc taagggggtt atagggggtc tttttcccct
       61 tatagccggg gctataaaaa ggcagtgccc ctcatagttc tattatttgc gaccaccgtt
      121 cttttacatc catcgatgat gatttttagc aaaaattaag tctcctacag aagtggtaac
      181 atagacgatt ttagagaagc aatttttacc ctttacgaga cttacgctgg aaagagtaag
      241 attatagaga cccccacaga atcagtaaag agttagtata atatcgagcc attcactgtt
      301 tcttccactc tctggaaacc aaaatggaat aatgaaagtt ttgcaaccct gaaactggta
      361 aacctgtaaa agagattatg aatgctatga ataaaagagg ggggaaacta tgtttatgaa
      421 aaggtgtatc ctatcttacc attaatgcta taggtgcctg aggtgccatt attttacttc
      481 tataaagtga tgtccatctt cctcttttat ttctccccac tgataaggga taggataatg
      541 gtaaagaggg ccatcgacca ccaaagtgtg gaactcccct ttttctccac aagggtcaat
      601 gcctaaattt accaattctt caatcagaga ataatcatag cgcctaccca ggtagcgaga
      661 atctaaaaat tggtcgttca ccattattac aaaggctaca aaccctcttt ttactatttc
      721 ctgagccacc ttctggtgtt cttctcccca aagaggaaga aggggataga taccttcctc
      781 tttgcaaact ctctctaccc actcccgatg ttcagccaaa tcgatgtctc caaatactcc
      841 actattaatt ccttttcctt taaggaaccg gatggtctcc ttaaagttat cttcgtattc
      901 ctcccaggtg gcattagctt tctggagttc taaacccata gcctgagctt gcctttccag
      961 aataaaagaa ggaagatgat gagctcgaga aaacgcaccg tcttcttgaa gcatagtaaa
     1021 aagaaatctc acttcccatc ctgtttcttg cgctcgaaga atgctcaaat gacaatcttt
     1081 acccccgcta aaagaggcaa aagctttgta gtccaaatct ttacctcctt ttaatttatc
     1141 atgccaaatt aaatttaaga gcttccctca tcctatttat tttcccgaag taagtcaata
     1201 ctgcaagttc ccctataacc tcatcatgtc ccataatttt tctctggaca ctattatggt
     1261 taaccgcaaa acctaatata aaatataact gttaccttcc cgcgagggtc gcattgtcac
     1321 atcccgtggt gatttctttt tttcaaaaac gagattgcca cgtattgatg ctacgaggca
     1381 aacgcaaaaa gctaaaaaga ccatcgaaaa agtcttttat agctaccgag agggtttctt
     1441 catagggaac attaccgcgg cttttgtggt taactataat ttgtagtata ataaggagtg
     1501 aatccaaaaa aggtgaaagg ccatgaaaag agattattat gaagtattag gagtaaatag
     1561 aaatgctacc tcagaagata taaaaagagc ttatcgtcgc ttggctcgcc aatatcatcc
     1621 tgatgttaat caaggagata acggtgcagc agaaaaattt aaagagatta atgaagctta
     1681 ccaggtactc tctgatgagc aaaagagagc tacctatgac cgttttggcc actctgcttt
     1741 tgagggagcc aatcagggaa gctatggggg gtttgggtca gattttggcc cctttggtga
     1801 tttttccaac tttggcgata tattcgactt cttttttggt ggaggaactc aatcccgcag
     1861 agagcatcgt cctagagcag caaaaggacc ggatataaaa gtagaaatta ctttaggatt
     1921 tgaagaagca gctcaaggag tagaaaaaga aatttctttc tcccgaacag agatttgtcc
     1981 agaatgtcag ggaataggag gaaagagaaa ggttgcctgt cctcattgcc agggaagagg
     2041 agaagttcgt catacacaaa cttctttatt cggttcagta gttacctccc ggccttgctc
     2101 ctattgtcag ggaaaaggat gggtagcaga agatatttgt tccctttgtc gaggaagcgg
     2161 taaaatcaaa aatgaaagaa aaattaaaat taaaatccct gctggcgtgg atagcggtta
     2221 ccgcttacgg gtggccggag aaggagaagc gggagaaaac ggaggaccat cgggagactt
     2281 atacgcttat attaaggtta aacctcaccc agttctagaa agaaaaggtt cggacctcta
     2341 ttatgatctg gagctgagtt atccacaact ggtcctgggt gatgaagtag aaatacctac
     2401 tttgcgaggt aaagagacca tccgtattcc tgccggaacc gaaagtggag ctttgttgcg
     2461 tttgcggggt aaaggattac ctgaccccca gattggtagc cggggagatc agataatcag
     2521 agttaaactc cgtgtacccc gtcggttgac cttagaacac cgacaagctc tggaaaaact
     2581 catggaactg gagcgagtag aaaacagtct ggaaggaaac ggcgacaatc gtaaacagag
     2641 cggagtattt gagcgcttaa aggaggcttt tactcgccct gagtaatagc ttccctcatg
     2701 agccgttttc tttttcactc ccctttggta atcggtgaaa caattttact ctcaccgcgg
     2761 gagagtcatc atcttttagt agaaagaata aagcccggtt cttctattct cttgtccgat
     2821 gggaaaggaa acctatttcg gggaacttat gtggaagaaa aaagaggaag agcccggata
     2881 cgagtagaag agaaagttga agaagaaaaa cggagcttta atctcaccgt atggcaagcc
     2941 attttgaaaa gcccctctcg tatggattgg ataatagaaa aattaaccga gataggagta
     3001 tcacaaatag gttttttctc ctcccatcgc tcacttaaaa atgttatctc tccggaaaaa
     3061 atggaaagat gggagaaaat cgccctgagt gcttgcaagc agtcaggaag gacaattttt
     3121 cctgatttag tatttttgga gcaatgggaa ggtttcgtct ctcacctcag tagctacccc
     3181 ggagtaacga ttcttgccga cccggaagga gatgaaactc tctggtcttt tctccagggg
     3241 aaaaaagaga gaaattttca aatagtgata gggccagaag gcgacttaac tgaagaagaa
     3301 aaggaagaga ttttagaaaa aagaccggga aaacggataa aattaagtaa aaaaattttg
     3361 cggagcgaaa cagccagtct tttttctgcc tcggtagtaa cttcttttct ggaggataaa
     3421 tgaaagtagc gataaaaact ctaggatgca aagtaaatca agttgaaagt gatatactgg
     3481 ctaccacctt ttcccaggag ggagtggaag tggtgagttt tggagaagaa gctgacctct
     3541 acatagtgaa tagttgtgca gtaaccaaag aagcagagaa taaaactcgc cagctggtaa
     3601 atagaatttt gcgaaaacac ctcgctcccc ggataatatt gaccggttgc tatgcctacc
     3661 ggatttcccg ggagagagaa aacccatttg aagaatcggt ttttttacta cctgattttc
     3721 ccaaagaaaa agaaattata aacttagtgg gaaaatggtg gggggaaaag tttacccttg
     3781 ctgaatatcc cctcccctct cgggcccgtg cctggctcaa agtagaggat ggttgtgacc
     3841 acttttgtag ctactgttta attcctcatc ttcgaggcaa gataaaaagc gaggaaaaag
     3901 aaaaaattat aagacaggct caagcacttg aaagagaggg aataagagaa atagttcttt
     3961 gtggggtgaa tttaggctat tatggaaaag atagaggaga aaaggggctt attaatcttt
     4021 tagaagaatt agtagataat accaccctgg tgcgttttcg attgagttct attgaaccat
     4081 tcctgctgga tgaagagttc gttaaacgtt acttttcgct tcgagataga gtttgtcctc
     4141 atttccacct tcccttacag agtggtagcg acagggtttt gaaaagcatg aggagaggat
     4201 acgatactac tcgctaccga gctcttgttt ctctactgcg aagttactgt cctgcggtcg
     4261 ccatctccac cgatatcata gtgggattcc ccacagaaga aaaagaggat tttgaaaaca
     4321 cagttaattt ttctcaagag ataggttttt ctcgaactca cgtttttgtg ttttctcccc
     4381 gcccgggaac accagctttt ctctgggaaa aagaaagagg gactcccaaa aaagagaaaa
     4441 aaaaccgaga aaaaatcctt ctacaagtgg ccagagaaac tcaaaaaaaa tattaccatc
     4501 tctttttagg taagaaactg aaagtcttag tagagtcaat acaagatgga acgagctcgg
     4561 gttattctga aaattacatt ccggtcaggt ttagaggaaa ttctaaagag agagaaatag
     4621 tagaggtaac aatagaaaaa gaaggggacg gtttcttaga agggaaacta tgagattaat
     4681 attacctata tctatctggt ctggacacaa aacggcggtt tttatcctta aataatttgg
     4741 attcctgata gagacattca ggaatgacgg acttcaagag acaccctcac cctaaccctc
     4801 tcccttcgag ggagagggaa agaagatggg gtggtctcct attaaggaag agggaaagaa
     4861 gagataattt aagggctccc cttttcgtca ttcccga
//
LOCUS       DAOSVE010000107         4897 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_7573_length_4897_cov_69.230277, whole genome shotgun sequence.
ACCESSION   DAOSVE010000107 DAOSVE010000000
VERSION     DAOSVE010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7573_length_4897_cov_69.230277"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            360..1373
                     /locus_tag="PK016_07710"
     CDS             360..1373
                     /locus_tag="PK016_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00303 family protein"
                     /protein_id="HXL02980.1"
                     /translation="MSFLKEEVNVFILIIGGTELGTIPGLSAAGSNPQMVAYTAPADA
                     EVLWRGKPQIIDALPVDPEGHPTPALISRAAYLEARFPVLIVRAGTFLPPTVPYVETS
                     ASPGKDPRQGKAVEEAFSLWEKGRRLGEELSKTQSYLVIAESLPGGTTSALCVLRALG
                     YNGMVSSAFPHNPVPLKEKIWQEVSFFQSIGYGSFRGEGLKAVESCGDPMQAVAGGLV
                     EGALSQCQVVLAGGTQMLAVSAVLRHQGYSRPLFVATTRYVIEDKSAHFQEIAQELGV
                     EVYSAPLDFSHSPFPGLLDYERGFVKEGVGAGGAVWYAEKLGSSVKTIISRVEELYGN
                     FFP"
     gene            1398..1763
                     /locus_tag="PK016_07715"
     CDS             1398..1763
                     /locus_tag="PK016_07715"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HXL02981.1"
                     /translation="MREQKICWVIILSLFVAAGIWVGVKCVPSRGSYTILLETQDQTM
                     ELKVTPQTEKVITLKGPLGETIVTVKGGKVWVSSSPCPDQTCMKMGKIPDNGGFIACI
                     PNRVIIRKKGAKTLPAFQQ"
     gene            1771..2517
                     /locus_tag="PK016_07720"
     CDS             1771..2517
                     /locus_tag="PK016_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXL02982.1"
                     /translation="MTAKAASGRVTSFLHRSSAIEDLHKVDHLVKTEKRLLRCIISSY
                     SAQGAYQAVANAGLAGVVKISAWDATEDLIKALKEGQVDLVLAENLNVYSNIFLGREK
                     VKNLLGVIPVLDHEYMMEESQKVLSRLGIEIPSLKNRIQNLSGGQRQSVAISRSVYWN
                     AKLLILDEPTAALGVTEQKKALDLVRTLSSQGVAVIIISHQLYDVFQVAHRLVVLRRG
                     EKVGERLTKETNSDEIVGLMVGAELVGEKK"
     gene            complement(2520..2678)
                     /locus_tag="PK016_07725"
     CDS             complement(2520..2678)
                     /locus_tag="PK016_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HXL02983.1"
                     /translation="MGRYVCLICGYVYDPRQGDPDFGIEPGTPFSQLPEDWICPVCGA
                     SKDQFEKE"
     gene            complement(2785..3678)
                     /gene="garR"
                     /locus_tag="PK016_07730"
     CDS             complement(2785..3678)
                     /gene="garR"
                     /locus_tag="PK016_07730"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002917952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HXL02984.1"
                     /translation="MTTKPVIGFIGLGIMGKPMSHNLLQAGYPLVVYTRTKTKIAEMV
                     KEGAQGATTPREVGEKSKIVITMLPDSPQVEEVILGENGVLEGVDKGEIVIDMSSIDP
                     FSTKQIGEALSLKGVEMLDAPVSGGEEGAIKGELSIMVGGKREIFDKCLPIFQVLGKN
                     ITYTGELGSGEMTKLVNQIIVAVNLAALCEAFTLGAKAGLKAETIYQAIRGGMAGSKV
                     MDMKVPRFEERNFTPGFKIDLHLKDLKNALSTAREMRVPLPFTSGVEQIMIALSGKGK
                     GDKDHGAMIEFFEELSQVEVK"
     gene            complement(3692..4618)
                     /locus_tag="PK016_07735"
     CDS             complement(3692..4618)
                     /locus_tag="PK016_07735"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HXL02985.1"
                     /translation="MAFSIGIDLGGTKIAGVLTDEELNILAEEKIPVDREKRVDKVIG
                     DMELLIRNFKEKGKGECLGIGLALPGLLDWKKGGIVLSENLNWRNVPVIPLLKEKLSL
                     PFLMEHDVRSGAIAEVYFGAARLFQDILYVSVGTGIAGSLIWKRNIIRGAHEASAELG
                     HMVIEPDGPLCRCGNVGCLETLASGNAMERDVFHLSGEYMKGEEIFKRAQEGIFPYQE
                     VVKRASSFLGLGLFNLVQIFDPEVIILGGGVAEAGDWWLEMVKEGYENRFFWTPTIPP
                     LVLGQFRGKASVMGAAGLPILQKRGEYHQSVY"
BASE COUNT         1354 a         1075 c          996 g         1472 t
ORIGIN      
        1 ccagatggaa cagaaatccc tctgagtgta agtattggta tagcttctta tcctcaagat
       61 gcagaagatg aagaaaaact cttctcttta gcagatactg atatgtatag agctaaaaca
      121 atgaagaaac aaaccctacc tttcttttaa aagagggaga aatagaagat tagtagtcgt
      181 taacaaatct ggctaagccc tttttctcta tgtgtaacaa ccttgagttt ttgacttccg
      241 atgtaattta ctttttaaaa gttttaatat tttaatcagt tttgcgggat actataatct
      301 ctttctttca gctttttgtg ctctttccct ttcttctcag aagtgattaa attgtaccaa
      361 tatcttttct taaagaggag gtaaatgtgt ttattctaat cattggagga actgaattag
      421 gaactattcc cggactttca gcagctggct ctaatccaca aatggtggct tatactgctc
      481 cggctgatgc cgaagtgctc tggaggggta aaccccaaat aatcgatgct cttcctgttg
      541 acccggaggg acatcctacc cctgctctaa taagtagagc agcttacctt gaagctcgtt
      601 ttccagtgct tatagtacga gcgggaactt ttcttccccc tactgttcct tatgttgaga
      661 cttctgcttc tccagggaaa gaccctcgac aggggaaagc agtagaagaa gccttttcac
      721 tctgggaaaa aggaagaagg ttgggagaag agctctctaa aactcaaagc tacctagtga
      781 tagctgaatc tttacctgga ggaactacct cggctctttg tgtccttcgg gctttgggat
      841 ataatggaat ggtctcttct gctttccctc ataaccctgt tccccttaag gagaaaatat
      901 ggcaagaagt tagctttttt caaagtattg gctatggttc ttttcgaggg gagggattaa
      961 aagcagtgga gagctgtgga gaccccatgc aagcagtagc agggggcttg gtagagggag
     1021 ctctatcaca atgtcaagta gttttggctg gaggaactca aatgttggcg gtgagtgctg
     1081 tgcttcgcca tcagggttat agtcgccccc tttttgtggc tactactcgc tacgtgatag
     1141 aagataaaag tgctcatttc caggaaatag cgcaagagct gggtgtagaa gtttactctg
     1201 ctcctctgga tttttctcat tctccctttc ctggactttt agattacgag cgagggttcg
     1261 taaaggaggg agtgggagca ggaggagcag tatggtatgc agaaaaactg ggtagttcag
     1321 taaaaaccat tatttcccgg gtggaggagc tctacggaaa tttttttccc tgaaggtctt
     1381 gataaaggta atagttgatg agagagcaaa aaatatgttg ggttattatt ctatctctat
     1441 ttgtggcagc agggatatgg gtaggagtaa aatgtgtccc atctcgaggt tcttatacca
     1501 ttttattaga aacccaggac cagactatgg aactgaaagt tactcctcag acagaaaaag
     1561 ttataactct gaaaggaccg ctgggagaaa cgatagtaac agtaaaggga gggaaagtat
     1621 gggtcagttc ctctccttgt ccagatcaga cctgcatgaa gatggggaaa atccctgata
     1681 acggggggtt tatcgcttgt atacccaatc gggtaataat caggaagaaa ggagctaaga
     1741 ctttaccagc ttttcagcaa taaaataatt atgacagcaa aagctgcttc cggaagagtc
     1801 acatcttttc tccacagaag tagtgcaatt gaagatttac ataaggtgga ccaccttgtc
     1861 aagacagaaa agagactttt aaggtgtatt atttcctcct atagtgctca gggagcttat
     1921 caggcagtag ctaatgctgg gttggctgga gtggttaaaa tttctgcctg ggacgccact
     1981 gaagatttaa ttaaagccct aaaggaaggg caagtagatt tagttctggc agaaaacctc
     2041 aatgtttatt ccaatatttt cttaggtaga gagaaggtaa aaaatctgct aggggtaatc
     2101 ccggtgttag accatgagta tatgatggaa gaatcccaaa aagtattgag ccgcctgggg
     2161 attgaaattc cctctctgaa aaatcggatt caaaacctct ctggaggaca gcgtcagtca
     2221 gtagctattt ctcgctcggt atattggaat gctaaactcc ttattctaga tgaacccact
     2281 gctgctttgg gggtaaccga gcaaaaaaag gcgctggatt tggtgcggac tttgagctct
     2341 caaggagtag cagtaatcat aatcagccac cagctttatg atgtgtttca ggtagcacac
     2401 cgcttggtag tattgcgacg gggagaaaaa gtaggggagc gcctgaccaa agaaaccaac
     2461 tctgatgaaa tagtggggct tatggtaggc gctgagttag tgggagagaa aaagtagaac
     2521 tactctttct caaactggtc tttacttgct ccacagacag ggcaaatcca atcttccggt
     2581 aattgagaaa aaggagtacc tggttctata ccaaaatcag ggtctccctg ccgagggtca
     2641 taaacatatc cgcaaattaa gcaaacgtat ctccccattt ctatgctcct tccccatatg
     2701 aatgatgact gaaagatgga gaaatgataa aaaaatctgg ccagttagtc aagagctttt
     2761 acttgtgata taagcttttc tcgattactt tacttctacc tggctcagtt cttcaaagaa
     2821 ctctatcatt gctccgtggt ctttatctcc ttttcctttt cccgataaag ctatcataat
     2881 ttgttccact cctgaggtaa aaggaagagg gactctcatc tctcgggcag tagataaagc
     2941 attttttagg tctttgagat gaagatcgat tttaaatccc ggggtaaaat ttctttcctc
     3001 aaagcgagga actttcatat ccataacttt gcttcctgcc attcctcctc ttatggcctg
     3061 gtatatagtc tctgctttta gccctgcttt ggccccaaga gtaaaagctt cgcaaagagc
     3121 ggctaaattc acagccacaa ttatctgatt gaccaattta gtcatttctc cgcttcccag
     3181 ctctcctgtg taagtgatgt ttttccccag aacctggaag ataggaagac atttatcaaa
     3241 tatctctctt ttccctccta ccataatgga aagttctcct ttaattgctc cttcttctcc
     3301 tccactcact ggagcatcca gcatttccac tcctttcagg gaaagggctt ctcctatttg
     3361 tttagtagaa aaggggtcaa tagagctcat gtcaattact atttcccctt tatccacccc
     3421 ctctaaaact ccattttctc ccagaattac ttcttccact tgaggagaat cagggagcat
     3481 ggtaatcact atcttgcttt tctctcccac ttcccgggga gttgttgctc cttgtgctcc
     3541 ttctttaacc atctctgcta tcttagtttt agttcgagtg taaactacta aaggatagcc
     3601 agcttgcaaa agattgtggc tcataggctt tcccataatt cccagaccaa taaatcctat
     3661 tactggtttc gttgtcattt tatttctcct ctcagtaaac tgactgatga tattctcctc
     3721 ttttctggag aatgggtaac cccgctgctc ccataacact ggctttacct cgaaattgcc
     3781 ccaaaaccag gggagggatg gtgggtgtcc agaaaaatct attttcatat ccttctttaa
     3841 ccatctctaa ccaccaatct cctgcttctg ccacccctcc tcccagaata attacctccg
     3901 ggtcaaatat ctgcaccaga ttaaatagtc ccaggcccaa aaaactgctt gctcttttaa
     3961 ccacctcttg ataaggaaaa atcccttcct gcgcccgctt aaaaatttct tctcctttca
     4021 tatactcacc gctcaggtgg aaaacatctc tttccatggc atttcctgag gctaacgttt
     4081 ctaagcatcc gacatttcca caccgacaca gaggcccatc tggttcaatc accatatgtc
     4141 ctaattcagc agaagcttcg tgagcacccc ggattatatt tctcttccaa attaaacttc
     4201 cagcaattcc tgttcccaca cttacataaa gaatatcctg gaaaagacga gcagcaccaa
     4261 aatatacctc tgctatggct ccgctgcgga catcatgctc cataaggaag ggaagagata
     4321 atttttcttt caggagagga atgactggca cattacgcca gttcaggttt tcagataaaa
     4381 ctatacctcc ttttttccag tccagaaggc caggaagagc aagacctatc cccagacatt
     4441 caccttttcc tttttcctta aaattccgga tgagaagttc catgtcccct ataactttgt
     4501 ccactctctt ttccctatct acaggaattt tctcttcagc caggatattc aattcctcat
     4561 cggtgaggac tccagcaatt ttagtgcccc ccaaatcaat cccaatagaa aaagccaaag
     4621 ttcatccccc tttcttttag ctttttaaaa tctcttaatt attgtgagat agtggcgatg
     4681 aaaattcaag gtttcatgga ggctgtttga aaaatagcct tctagatttt tatggtcact
     4741 caaaaactgc agtagctgtt cttctttgta attatagtta atcgtaaagc tcataaaatc
     4801 ttttcctatt tatagtatct cgcaaaactg gttaaaaact ttaggtcgct acgcatagag
     4861 aaagactttg tctttctcta tggcctttat ttctttt
//
LOCUS       DAOSVE010000108         4746 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_7810_length_4746_cov_64.995737, whole genome shotgun sequence.
ACCESSION   DAOSVE010000108 DAOSVE010000000
VERSION     DAOSVE010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7810_length_4746_cov_64.995737"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            376..2151
                     /locus_tag="PK016_07740"
     CDS             376..2151
                     /locus_tag="PK016_07740"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HXL02986.1"
                     /translation="MPLIKIPLLNRVREVKKGEKLLEALQREGIEIDSYCGGMGSCGK
                     CVVKIKGEGVSSPNSLEEKHLKEKIKEGFRLACQVEVYGEIEVDVSPSLISQVEVMVK
                     TWEARREELPVYQTKASVVKPLVDGVSSLQEILVSQIQGEGKKWSLASLYRLSHLGEE
                     RETQFEVISDEEKIRYVSELSPDKSFLGMAFDLGTTTIGGELVDLTTGTSLHRLGALN
                     RQTQFGADVISRLRAIQDDSYNLYRLQEVLAQTMNDLIQEACEKVNKNPERIFAVTVA
                     GNTIMEHLFLGVSPLSIGVAPYTPVFRKEQVLDAREVKIVVNPEAQVYLFPSLAGYVG
                     GDILSGVVAFNLMEKEGLTLYVDIGTNGEIVLIDGENVFCCGTAAGPAFEGAQIKYGT
                     RATLGAISAVEIKDGDVKIYTIGDLAPRGICGTGLIDALSCFLREGLLSPTGRFKEND
                     KWSNRLIKEGGNRAFLLSYDPPIFITQEDISQLQLAKAAIQAGREILLKEAGRKEEEI
                     EEVILAGAFGSFINPESAKMIGLIPREAKTTSVGNASLYGAKKALLSRDFCQKVKTLF
                     QRARYIELSARSDFSDYFYQSLIFD"
     gene            2174..3472
                     /locus_tag="PK016_07745"
     CDS             2174..3472
                     /locus_tag="PK016_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HXL02987.1"
                     /translation="MAVVKPFRGYFYSSEFIKSHPIECLAAPPYDVISEKEKVRLEEE
                     PYNFVHLTLGKEGNSYQKAKELLQSWVKEGVVEQDKTPSFYIDEQEFQLNSLERKKRT
                     GFVALVRLESWEKKIIMPHERTMPKYSLDRLELLRATRANLEQIFGIFNDSSRGIDEL
                     LEENKKEENFIFQFTDYQDVTHRLWRIEEKEVINKIRRILNPRTIIIADGHHRYETSL
                     MYREERRKELQDPWEEIPEDYVMMTLVNMKNPGLLILPTHRLVHGLPEERVKGFFSRC
                     ANYFTVKNFSNEREMEEFLRSAPPHTIGVYDKVENKWGAITLKDLKVMDEKLGKENVN
                     RYLDTAILHEVVFKEILGINRDGEGESEYVDYLRGTKDVFALAREENYYQLVFVMRST
                     PLERVEEAVAYAQRMPQKSTYFYPKAWSGLVFRLLDEEKL"
     gene            3469..4122
                     /locus_tag="PK016_07750"
     CDS             3469..4122
                     /locus_tag="PK016_07750"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HXL02988.1"
                     /translation="MKPYFQLALDIVTREEAKQLALSLLDYIDIIEVGTPLILRYGLS
                     IVTEIKNLVGKKKVFADTKIVDAGSWETEEALKSGADMVSVLAGASFFTLREAKEVCQ
                     SRGARLVVDNIDFFFPCPQKVKEIKEIDPDFLSLHLASDAFHKGENWLEAMEKSPFLS
                     EKGFAFMVAGGISLSSLPRIIKEISPSVVVVGSAITNSPHPEEMAKEIREIIDEFGL"
     gene            4106..4675
                     /locus_tag="PK016_07755"
     CDS             4106..4675
                     /locus_tag="PK016_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HXL02989.1"
                     /translation="MSLGFDQAKEAVLKEADALLSTLDSNKVKRFIARLEGDWRRRRL
                     FFWARGRSFLVLQGFAVRLMHMGYEVHIVGEVNCPAIGGGDILVVASGSGGTSSVLLM
                     VEKAHQFGAEVVAIVGRPGSSLQEKADEVVEFNPEKAGDSVQLFSNGGGTRFEHSLML
                     FFDSIILSLVFPRREEAYREMMERHANLE"
BASE COUNT         1418 a          774 c         1134 g         1420 t
ORIGIN      
        1 tttacttctt ttctatcttc tatccgtggc tttcctaata aggaataaag tataagggtc
       61 tttcctctat agttctggca attcctttct ctgtctttgc gaggatcgct cttacgtttc
      121 ctgtggcaat tttggttttt tctcttctgt aaaaacaaaa gcagatcgcc acgacgttgg
      181 gatatttttg ccagcgtagt tttttaatct ttgttttaag ttttttatcc tacatcatag
      241 attgctctat actgtgctca tggtgataaa aagggggcac cgtaggctat tcttgacaaa
      301 aatgataaac taacttgata gaaatcttga agagcctaaa gaggatttga gaaagaagga
      361 tagataaata ctaaaatgcc acttataaaa attcctcttc ttaatcgagt tcgggaggtt
      421 aaaaaaggag aaaagctgct ggaggcttta cagcgagagg gaatagagat agattcctac
      481 tgtggaggta tggggtcttg tggaaaatgc gtggtaaaaa tcaaaggaga gggggtatct
      541 tctcctaatt ctctggagga aaaacattta aaagaaaaaa ttaaagaggg ttttcgttta
      601 gcttgccagg tggaagtata tggagaaata gaggtagacg tttctccctc tcttatttct
      661 caggtagaag tgatggtaaa aacctgggaa gcccggcgag aagaacttcc tgtctatcaa
      721 acgaaagcaa gtgtggtaaa accgctcgta gacggagtat cttctctcca ggaaattctg
      781 gtttctcaaa tacagggaga aggcaagaag tggtctttag cttctcttta tcgtctttct
      841 cacctcgggg aagaaagaga gacacagttt gaagtgataa gtgatgaaga aaaaatccgg
      901 tatgttagtg agctttctcc cgataaatcc tttttaggga tggcttttga cctgggcacc
      961 actactatag gaggagagct ggtagacctc acaactggta cttctcttca tcgtttagga
     1021 gctctaaata gacagactca atttggggca gatgttatat ctcgtctccg ggctatccag
     1081 gatgactctt ataaccttta tcgtttacaa gaggtgttag ctcaaactat gaacgattta
     1141 atccaggaag cctgtgaaaa agtgaataaa aacccggaaa ggatttttgc tgttactgta
     1201 gctggaaata ccataatgga gcacctcttt ctgggggttt ctcctctttc tattggagta
     1261 gctccttaca ccccggtttt tcgaaaagag caggtgctcg atgcccggga agttaaaata
     1321 gtggtcaatc cagaagccca agtttattta tttccctctc tggctggtta tgtgggggga
     1381 gatatcctgt ccggagtggt agcctttaat ttgatggaaa aggaaggttt aaccctttat
     1441 gtggatatag gcactaatgg ggagattgtt ttaatagatg gggaaaatgt tttttgctgt
     1501 ggaacggctg caggtcctgc ttttgaagga gcacagatta agtacgggac tcgagctact
     1561 ttgggagcga tatcggcagt agagataaaa gatggagatg ttaagattta cactattgga
     1621 gatttagctc ctcgggggat atgtggtact ggtttaatcg atgccctatc ttgttttttg
     1681 agagaaggat tgctgagccc aactggaagg tttaaagaga atgataaatg gagcaaccgg
     1741 ctgataaaag aaggaggtaa tcgagctttt cttttaagtt acgaccctcc tatttttatt
     1801 actcaggaag atatttctca gttacaattg gctaaggcgg ctattcaggc cggcagagag
     1861 attctcttaa aagaggcagg aagaaaagaa gaagaaatag aggaagtaat attagcagga
     1921 gcttttggta gctttattaa cccggagagt gctaaaatga ttggtcttat ccctcgggag
     1981 gctaagacta cttcggtagg taatgcttct ctctatggag ctaaaaaagc tcttctttct
     2041 cgagattttt gccagaaagt taaaacactt tttcaaagag cccggtatat tgagctttcg
     2101 gcaagaagtg atttttctga ttatttttat caatctttaa ttttcgatta gactggaaaa
     2161 ggagacaggg aagatggcag tagttaaacc atttcgaggt tatttttatt cttcagaatt
     2221 tattaagagt catcctatag agtgtctggc agctcctcct tatgatgtga tatcagaaaa
     2281 agaaaaagtc cggttagagg aagaacctta taattttgtc cacctcaccc tggggaaaga
     2341 aggaaatagc tatcaaaaag caaaagaact gcttcagtcc tgggtaaaag aaggagtggt
     2401 agaacaagat aaaactcctt ctttctatat agatgaacag gagtttcagc tcaactctct
     2461 ggaacgaaaa aaaagaactg gttttgtagc tctggtacgc ttagagagct gggaaaagaa
     2521 aataattatg cctcatgagc ggactatgcc taaatattcc ctggatcgat tagagctctt
     2581 gcgagctact cgagccaatt tggagcaaat attcgggatt tttaatgatt cttcaagagg
     2641 aattgacgag cttttagaag aaaataaaaa agaggaaaat tttatttttc agttcactga
     2701 ttatcaggat gttacgcacc gattatggag gatagaagaa aaagaggtaa taaataaaat
     2761 aagaagaatc cttaatcccc gtactattat cattgctgat ggtcatcatc gctatgagac
     2821 cagcctcatg tatcgagaag aaagaagaaa agaactacaa gacccctggg aggaaatacc
     2881 cgaagattat gtgatgatga ctttagtcaa tatgaaaaat cccgggcttt taattcttcc
     2941 cactcatcga ttggtgcatg gactacctga agaaagagtg aaaggatttt ttagccggtg
     3001 tgcgaattat tttacggtta aaaatttctc taatgaacga gaaatggaag aatttttacg
     3061 ttctgctcct cctcatacca ttggagttta tgataaggta gaaaataagt ggggagctat
     3121 aactttgaaa gatctgaagg taatggatga aaagttggga aaagaaaatg taaatcgtta
     3181 tttagacacc gccattttac atgaagtggt gttcaaagaa attctgggga ttaatagaga
     3241 cggagaaggg gaaagcgaat atgttgatta tctccggggg actaaagatg tttttgcttt
     3301 agcccgggaa gaaaattact atcagctggt ttttgttatg cgctctaccc ctttagagag
     3361 agtggaagaa gcggttgcct acgctcagcg aatgcctcaa aaatctacct acttttaccc
     3421 taaagcatgg agtgggctgg tctttcgttt actggatgag gaaaaattgt gaaaccttac
     3481 tttcagctgg ctctggatat tgtaacccgg gaagaagcta aacaacttgc tctttctctt
     3541 ttagattata tagatattat agaggtagga actcctttaa ttcttcgcta tggtctttct
     3601 atagttacgg aaataaaaaa tttagtggga aagaaaaagg tatttgctga taccaaaatt
     3661 gtagacgccg gaagctggga aaccgaagaa gctttaaaaa gtggagcaga tatggtttca
     3721 gtactggctg gtgcctcttt ttttactctc cgggaggcaa aagaagtttg ccaaagtaga
     3781 ggagcgcggt tagtagtgga caacattgat tttttctttc cttgtcctca gaaagtgaag
     3841 gaaataaagg aaattgaccc tgattttctt tctcttcatc ttgcttctga tgctttccat
     3901 aaaggagaga actggttaga agcaatggag aagtctcctt ttctctccga aaaaggtttt
     3961 gcctttatgg tagcaggagg aataagcctt tcttctctac ctcggataat aaaagagatt
     4021 tctccttctg tagtcgtggt aggttcagct attactaatt ctcctcatcc tgaagaaatg
     4081 gctaaagaaa taagagagat aatcgatgag tttgggcttt gaccaggcaa aagaagcagt
     4141 tcttaaagaa gctgatgccc ttctttctac tctggactct aataaggtaa aaaggtttat
     4201 tgctcgtttg gagggagact ggcggaggag gagacttttt ttctgggcca gaggaagaag
     4261 ttttttagta ctgcaaggct ttgctgtgcg tctcatgcac atgggctacg aagtgcacat
     4321 tgtgggagag gtaaattgcc cggccatagg gggaggagat attttagtag tagcttcggg
     4381 ctcgggggga acatcttctg ttttacttat ggtagaaaaa gctcaccagt ttggggcaga
     4441 agtagtggct atagtgggaa ggcctggttc ttctcttcaa gaaaaagctg atgaggtggt
     4501 ggagtttaac cctgaaaaag caggagatag cgttcagctt ttttctaatg ggggagggac
     4561 tcgatttgag cattccctga tgctcttttt tgatagtatc attttgagtt tggtttttcc
     4621 tcgacgggaa gaagcttacc gagaaatgat ggaacgtcat gctaatttag agtgatgtgt
     4681 tctgtttttt taatatttaa attactattg aaagaagttt atagtatccc gcaaaactgg
     4741 ttaaaa
//
LOCUS       DAOSVE010000109         4743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_7817_length_4743_cov_78.888012, whole genome shotgun sequence.
ACCESSION   DAOSVE010000109 DAOSVE010000000
VERSION     DAOSVE010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 4743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4743
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7817_length_4743_cov_78.888012"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..820
                     /locus_tag="PK016_07760"
     CDS             23..820
                     /locus_tag="PK016_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HXL02990.1"
                     /translation="MSIKEGERVYLRLEDGKNYLLKVEKGKFFSTHLGNIYFDQIIGR
                     EFGEEIYTSQGKKVYLFQPGIVENVFHMKRKTQIVYPKDLGFILLMLDIKENDRVIDV
                     GLGSGAMCGALARLVGEKGKVYAYEKREEFISIARYNLSQWGWEERVEIKEKDIKDGF
                     EEREIDALFLDVPNPWDYLGECWKALRGGGRLAIVSPTAGQVMEVLRGLQTLPFRETE
                     VWENLFRQYKTNPSAFRPVDRMVAHTTYMIFSRKVLPSTLGFDLKEI"
     gene            845..1606
                     /locus_tag="PK016_07765"
     CDS             845..1606
                     /locus_tag="PK016_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXL02991.1"
                     /translation="MSIVVAVANQKGGVGKTTTCVNLGACLAEKGKKVLLVDIDPQGN
                     ATSGLGIDRQRLKQCVYDLLINGEKARGLMVETGVSGLWLLPATIRLAGAEIELVPLM
                     GRESRLKEGLREVIPDFDFVFIDCPPSLGLLTVNALTSAHKVIVPIQCEYYALEGLGQ
                     LMNTFHLVHNFLNPQLELWGVLLTMFDSRTNLSQQVVEETRRVLGDKVFRTVIPRNVR
                     LSEAPGFGKPIIVHDANSVGAIAYRELAEEVLENE"
     gene            1599..2429
                     /locus_tag="PK016_07770"
     CDS             1599..2429
                     /locus_tag="PK016_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HXL02992.1"
                     /translation="MSKRGLGKGLEALIPGAGAFGARTIQEIDIDSIKVNPFQPRTQI
                     DEEGLEDLANSIRVHGVLQPLLVRKMEEGFELIAGERRWRAAKRAGLSTVPVIVEEVE
                     EESRLEIALVENLQREDLPPLDLARGIKKLMEDFNLTQEEVAERVGKKRSTIANLLRL
                     FDLPPSIQDLLQEGKITLGHAKALLSVESQEKQEELVEEILAHSLSVREVEKKVKKVT
                     SQEEPSECSAEEKNFQELLTRYLSARVKVGKGKIIIEYHGREDVERLYSLIVGKEETF
                     "
     gene            2539..3603
                     /locus_tag="PK016_07775"
     CDS             2539..3603
                     /locus_tag="PK016_07775"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HXL02993.1"
                     /translation="MLGKKEEEQFTIFWIHPTKGQLKRFHFSWSTLLLGALVLVIGVG
                     LGIGGVLWYQSRIKKELYLTLEKMEETQKNTAILEAQKKEQEEKLKQLTQETEAVIKE
                     LETLRELDEKVRGVLEKDLQSRFQKLGIDLSLSSVSVPSYIPLDRFLGELYVVPLGMG
                     GPERLSSISLIRNSSPPASFNHDSGFERRAKALEDNLSWVRTETLARKKSLEEVVEVA
                     QQRDRLLYVVPMRWPTWGRVSSNYGWRNDPFTGRKAWHTGVDIAGPTGRNVVATAEGK
                     VIFTGWNGNYGKCVIVRHQFGYETVYGHLSKILVNTGDVVKKEQIIGQVGSTGRSTGP
                     HLHYEVRRYGDVINPWPYLP"
     gene            3635..4105
                     /locus_tag="PK016_07780"
     CDS             3635..4105
                     /locus_tag="PK016_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02994.1"
                     /translation="MIRMAIVFLLCFSWPAWSQVAKVVITEVNIQELAGDISISVRGE
                     IQAEGGGSVFLQASTTYEDREYFTAPREIKYALSRETFSFSFSREDFELINLKENKIE
                     RLPQLVKGQNVIVYVYEKKITSSEGQTEEVRQDIERTGYALRGILAKASKTLEF"
     gene            complement(4491..4664)
                     /locus_tag="PK016_07785"
     CDS             complement(4491..4664)
                     /locus_tag="PK016_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL02995.1"
                     /translation="MRYLLKTRREIVYEASKGYKKLSKKEKTERLDNLIAITGYNRDY
                     ICGYSPNLVGVFM"
BASE COUNT         1413 a          843 c         1156 g         1331 t
ORIGIN      
        1 ttctctggtt caaggagaga atatgtctat taaagaagga gaaagagtat atctgcggtt
       61 agaagacggg aaaaattatc ttcttaaggt ggaaaagggg aaatttttca gcacccatct
      121 gggaaatatc tattttgacc agataatagg aagagaattt ggagaggaaa tttacaccag
      181 ccaggggaaa aaagtttatc ttttccagcc tggaatagta gaaaacgtct tccatatgaa
      241 gagaaaaacc cagattgttt accccaaaga cctgggcttt atcctcctca tgctggatat
      301 aaaagaaaat gaccgggtca ttgatgtagg cttaggaagc ggagccatgt gtggagcgct
      361 ggcccgcctg gtgggagaga aagggaaagt ctatgcttat gaaaaaagag aagaattcat
      421 ctctatagct cgctataatc tttcccagtg gggatgggaa gaaagagtag aaataaaaga
      481 aaaagatata aaagatggct ttgaagaaag agaaatagat gctctttttc tggacgttcc
      541 taacccctgg gattatcttg gggagtgctg gaaagccctg aggggaggag gaaggttagc
      601 catagtttca cccaccgctg gtcaagttat ggaagtgttg cgtggcttac aaactcttcc
      661 ttttcgggaa actgaagtgt gggagaatct cttccgacaa tataaaacca atccttctgc
      721 ctttcgcccg gtagacagaa tggtggctca tactacctat atgatttttt ctcgaaaagt
      781 tttaccctct actttaggat ttgatttgaa agaaatataa aattagttta gaatatgtcc
      841 atctatgagt atcgtagtgg cagtggccaa tcaaaaaggg ggagtgggta agaccactac
      901 ctgtgtaaat ctgggagcct gtctcgcaga aaagggaaag aaagtgttgc tcgtagacat
      961 tgaccctcag ggtaacgcta caagcggcct gggaatagac cgccagcgat taaagcagtg
     1021 tgtctacgac cttttaatca acggagaaaa agcgagggga ttgatggtag agactggagt
     1081 ttctggtctc tggcttcttc ctgccactat tcgtctcgcc ggggcagaga ttgagctggt
     1141 tcctctcatg ggaagagaaa gccgcctgaa ggaggggtta agggaagtaa ttcctgattt
     1201 tgattttgtt tttatcgatt gtcctccttc tctgggtctt ttaaccgtta atgctttaac
     1261 ctcagctcat aaagttattg taccaattca gtgtgaatat tacgctctgg agggtcttgg
     1321 acaattaatg aatacttttc acctggtaca taattttttg aatccccagc tggagctatg
     1381 gggggtgttg ctgaccatgt ttgattctcg aaccaatctc tctcaacagg tggttgaaga
     1441 aacaagaagg gttctggggg ataaggtttt ccgcactgtt atccctcgta atgttcgttt
     1501 gagcgaagct cccggttttg gaaagccaat aattgtgcac gatgctaatt ctgtaggagc
     1561 aatagcctat cgagagctgg ctgaggaagt gttagaaaat gagtaaacga ggattgggta
     1621 aggggttaga ggcgttaatt ccgggagcgg gagctttcgg agctcgtacc attcaggaaa
     1681 tagatataga tagcatcaag gttaacccct ttcagccccg tacccagata gatgaggagg
     1741 ggctggaaga tttagccaat tccatccggg tgcacggagt tcttcagccg cttttggtga
     1801 gaaagatgga agagggattt gagttaatag ctggagagag gagatggaga gcggcaaaac
     1861 gagcgggttt atctactgtt ccggtgatag tggaagaggt agaagaagaa agcagattag
     1921 aaatagctct ggtagagaat cttcaacggg aagatttacc ccctctggat ttagcccggg
     1981 ggattaaaaa gttgatggaa gactttaatc tcactcagga agaagtggcg gaaagagtgg
     2041 ggaaaaaacg ctctaccatt gccaacttgc ttcgcctctt tgatttgcct ccttcaattc
     2101 aagacctttt gcaggaagga aaaataactt taggtcatgc caaagctctt ttgagcgtgg
     2161 aatcgcagga aaagcaagag gagctggtag aggaaattct tgctcattct ctttcggttc
     2221 gggaagtaga aaaaaaagta aaaaaagtca cttctcaaga agaaccttct gagtgctccg
     2281 ctgaagagaa aaattttcag gagcttttga ctcgttacct ttctgctcga gttaaagtag
     2341 gtaagggaaa gatcatcatc gagtatcatg gtcgggaaga tgtggagcgt ctttattctt
     2401 taattgtggg taaagaagaa actttttgaa gataagcttg aataattttt ttattttgtg
     2461 gtaacattta ggttaataat tgaaaagcag aaaataatgc acaccagtcg ataaaatttt
     2521 ttagggggag gatcgtaaat gctggggaaa aaagaagaag agcaatttac gattttctgg
     2581 attcacccca ccaaggggca gcttaaaaga tttcattttt cctggtcaac tctccttttg
     2641 ggagctttgg ttttggtgat aggtgtcggt ttaggtatcg gtggagtatt atggtatcaa
     2701 tcaagaatta aaaaggaact ttatcttact ctggaaaaaa tggaagaaac tcagaagaac
     2761 actgctattt tagaagccca aaagaaggag caagaagaaa aattaaagca actgacccag
     2821 gagaccgaag cagtgataaa ggaattagaa accttacgag aattagatga aaaggtgcga
     2881 ggtgttttag aaaaagattt acaatctcgg ttccagaaat tgggaatcga tctatctctt
     2941 tcctcagttt ctgttccctc ttatatccct ttagataggt ttttgggtga gttatatgtg
     3001 gttcctctgg ggatgggagg cccggagaga ctttcttcta tttctcttat ccgcaattct
     3061 tctcctcctg cttcttttaa ccatgattcc ggctttgaac gtcgggctaa agctttagaa
     3121 gataaccttt cctgggtacg aacagaaact ctggctcgta aaaaatcttt ggaagaagta
     3181 gtagaggtag cacaacaaag agacagatta ttatacgtgg tgcctatgcg gtggcccacc
     3241 tggggtagag tatcttctaa ttatgggtgg cgtaacgacc ctttcacagg aaggaaagcc
     3301 tggcacacag gggtagacat tgctggccct acagggagaa atgtagtagc cacagcggag
     3361 ggaaaggtga ttttcactgg ttggaatggt aattatggaa aatgtgtgat cgttcgccac
     3421 cagtttggtt atgaaacagt atacggtcac ctttctaaaa tcttggtgaa caccggggat
     3481 gtagttaaaa aagaacaaat cattggccag gtgggaagta cgggaagaag taccggccct
     3541 caccttcatt atgaagtaag aaggtatggt gacgtgatta atccctggcc ttatcttcca
     3601 tagaaaagga ggagaaaaaa cgaggcaaaa taacttgata agaatggcga ttgttttcct
     3661 tctgtgtttt tcctggcccg cctggagtca ggtagcgaaa gtagtgataa ctgaggttaa
     3721 cattcaagag ttagccggag atatttccat ttctgttcgg ggagaaattc aagctgaagg
     3781 tggagggagt gtatttttgc aagcttctac cacttacgag gatagagaat attttactgc
     3841 tccccgagag ataaaatatg ctttatctcg agagaccttt tctttttcct tctcccggga
     3901 agattttgag ctgataaact taaaggaaaa taaaatagaa agacttcccc agctggtgaa
     3961 aggacaaaat gtaatagttt acgtttatga aaagaagatt acctcctcag aagggcaaac
     4021 tgaggaagta cgacaagata tagaaagaac cggctatgct ctgcgaggga tactggctaa
     4081 agcttccaag actttagaat tttgaaagtg tctgctacct ttttatttta ggcggaacaa
     4141 aattacgatt tagttgtaaa gacctgcgat tattccttgg agagtcacaa aaacgctcct
     4201 cctgagtaga cccttttatc tgagagctat gaggtgaata ttcttttatg gctcgaaaga
     4261 tgcactttta cctcatcgca gttaaccacg ggatttgtgg cgaggagcgt gcagctttgg
     4321 cacaagccag ctcatacagg ggaatagtcg tttactacag ggatagtcca taatagccca
     4381 gatgtgcttt aagactttaa acacttcagt atcatatttc ctctttcggg agcacttttt
     4441 cttttttctt gcatcaccaa ttaatctata tgacttacca gagatatctt ttacataaac
     4501 acacctacca gattgggaga gtaaccgcaa atataatcac gattatatcc agtaatagct
     4561 atcaagttat caagtctctc cgtcttttct ttcttggata atttcttata tcccttactg
     4621 gcctcataaa caatttctct tcgtgtcttc aagagatacc tcacttatag cccctccctt
     4681 aagaatattc tcttaaggga ataataattt aactttgatt tttagttgag gcaactcgtg
     4741 agg
//
LOCUS       DAOSVE010000110         4711 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_7869_length_4711_cov_74.189003, whole genome shotgun sequence.
ACCESSION   DAOSVE010000110 DAOSVE010000000
VERSION     DAOSVE010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7869_length_4711_cov_74.189003"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..1437)
                     /gene="mnmE"
                     /locus_tag="PK016_07790"
     CDS             complement(40..1437)
                     /gene="mnmE"
                     /locus_tag="PK016_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HXL02996.1"
                     /translation="MKEIEGLLVNDTIVAPATPLGVGAIGIVRMSGREAVRIASRIFT
                     SRSGYIVENFVSHRLYLGDVFDPLTQEIIDEALLVVMKAPHSYTREDVVEFQLHGGPV
                     VIQKVLQLLLREGARLAEPGEFTRRAFLSGRIDLSQAEAVAEIIKARSEKALSLSLRN
                     LKGEWGKKIRFWENRLLNTLALIQAHCDFLDQEEELPLETIDEELTSLIEEIEKELSY
                     NERLKLAYDGALVVIVGKPNVGKSSLFNAIVGWDRAIVTSFPGTTRDALEENILIDGL
                     LLRLVDTAGMREGHDPVELMGIQKAEEYFEQADLVVVVLDQSVPLEKEDEVLADKVRN
                     KPHFVVLNKSDLLPQLNKSDISLLYPDEEVLEVSALQGGGIEKLLKKIGDKIKAEIGP
                     EDQAILINTRQKETLRKVFLFLTQARGNLKEGMSLDVVGLDLEGALREMGRMLGTQMD
                     KELLDAIFSQFCLGK"
     gene            complement(1406..2143)
                     /gene="jag"
                     /locus_tag="PK016_07795"
     CDS             complement(1406..2143)
                     /gene="jag"
                     /locus_tag="PK016_07795"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HXL02997.1"
                     /translation="MPRSVEVSGKSLEEALERAARYFDVSPESLNYEIISENRGFLSA
                     FTPRVVKVRVWIEEISREEAPRKEVGEKAPSSPAYLLQAGEELEKFFKELMNKMEMEV
                     DFQVQERDGFLFLLVEGKDAGMLIGKGGETLEALETLLRMVMSKKGFNDVILQLDISG
                     YREKREKALKKLAEKSAKKVMKEKRRLKLEPMNARERRIIHTSLKDYPEVTTYSIGKE
                     PGRRVVIDLKKERSSKRSEGNRRSVSK"
     gene            complement(2127..2912)
                     /locus_tag="PK016_07800"
     CDS             complement(2127..2912)
                     /locus_tag="PK016_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HXL02998.1"
                     /translation="MFSQLLDILAQAMEYLLRFFYNWTGDYGISIILLTLLIRLILSP
                     LIHKQTLSTRKMQEDMQELQPEIKKLQEKYGNNSQKLNEEMMKLYKEKGINPLGGCLP
                     GCLPLLIQLPILVVLYRVLMAYDYGQAGFLWLPSLSQKDPYFILPLLMGITTFWQQKI
                     SMPPTTDKAQAQQSMVMMVVMPVFLVFISWNLPAGVLLYWFVSNLFYILQQYVLNNQI
                     QKAKALSLKPEINAEVEPAISLESPSLTGSTVKKGGKTSAKKR"
     gene            complement(2933..3160)
                     /gene="yidD"
                     /locus_tag="PK016_07805"
     CDS             complement(2933..3160)
                     /gene="yidD"
                     /locus_tag="PK016_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HXL02999.1"
                     /translation="MKLSNFVVSGLDKVLLFYQKYISPLFPPSCRFEPTCSEYARQAL
                     RKYGLWKGGFLALRRLMRCNPYFPGGYDPVK"
     gene            complement(3147..3497)
                     /gene="rnpA"
                     /locus_tag="PK016_07810"
     CDS             complement(3147..3497)
                     /gene="rnpA"
                     /locus_tag="PK016_07810"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HXL03000.1"
                     /translation="MEGLSEKKDFSRLFSQGKRVKVDSIKLWYLQKNEGSLRVAFVGK
                     GKKAVDRNRVRRRLREAFRVCYYPQWRDKPVDFVFMSNEKLLKEGFKDLVETMGKVLN
                     EVEICGVKTESEIK"
     gene            complement(3501..3635)
                     /gene="rpmH"
                     /locus_tag="PK016_07815"
     CDS             complement(3501..3635)
                     /gene="rpmH"
                     /locus_tag="PK016_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012082253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HXL03001.1"
                     /translation="MKRTYQPHRIPMKRTHGFRARMKTQGGREILSRRRRKGRKRLTV
                     "
     gene            3968..>4711
                     /locus_tag="PK016_07820"
     CDS             3968..>4711
                     /locus_tag="PK016_07820"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HXL03002.1"
                     /translation="MKRKLRVAVICGGKSVEHEISLQSAKNVIEAMDGGKYDIVLIGI
                     DKEGYWHLKALTELQEEMKGSLLPHLTSQGDEVIFVPGGAGQLVNLSTGERLQPVDVV
                     FPVLHGPFGEDGAIQGLFKIAGVPFVGAGVLGSAIAMDKEVSKKLLREAGIPTPKFLS
                     FVCQQKEEIKFYEVANYLGVPFFVKPASLGSSVGISKVKKEEELRPALEEAFRYDGKI
                     LIEEYIEGREIECAVLGNEKPRASCPGEII"
BASE COUNT         1344 a         1115 c          870 g         1382 t
ORIGIN      
        1 gggcagcgcc ccctcatagt atgttccacg tggaacaatt tattttccca ggcaaaattg
       61 agaaaaaata gcatccaata attctttatc catttgtgta cccagcatcc gccccatttc
      121 tcgcaaggct ccttccagat ctaaacccac aacatctaag gacatcccct cttttaagtt
      181 gcctcgagcc tgagtcaaga aaagaaaaac ttttctaagc gtttcttttt gccgggtgtt
      241 gattaaaatt gcctggtcct caggacctat ttccgccttt atcttatctc ctattttttt
      301 cagtagcttc tctattcccc ctccttgcaa agcagatact tctaaaactt cctcatcagg
      361 ataaagcaag gaaatgtcgc ttttatttaa ttggggaaga agatcgcttt tatttagcac
      421 tacaaaatgg ggcttgtttc tcactttatc tgccagtact tcgtcttctt tctctaaagg
      481 gacactctgg tccaaaacca ccactacaag gtctgcctgt tcaaaatact cctctgcttt
      541 ttgtatcccc atcaattcca cgggatcgtg cccctctctc attcctgctg tatccactaa
      601 tcgaagaagt agaccatcta taaggatatt ttcttctaaa gcatcccggg tggttccggg
      661 gaaagaggtt acaatggccc tatcccatcc cacgatggcg ttaaaaagac tggacttgcc
      721 cacgttgggt ttacccacaa taaccacgag tgctccatca tacgccaatt taaggcgctc
      781 attataagat agctctttct ctatttcttc tattaatgag gtaagttctt catctatggt
      841 ttcaagaggt agctcttctt cttggtctaa aaaatcacag tgagcttgaa taagagccag
      901 agtattcaag aggcgatttt cccaaaaacg aattttcttt ccccattctc ctttcaaatt
      961 gcgaagagaa agagaaagag ctttttctga acgagcttta attatctccg ccaccgcctc
     1021 agcctgagaa aggtctatac ggccactcaa aaaggcccgg cgagtaaatt cccctggttc
     1081 agccaatctc gctccttctc ggaggaggag ctgcagaact ttttgaatta ccaccggacc
     1141 tccgtggagc tgaaattcca ccacatcttc ccgggtataa ctgtgagggg ctttcatcac
     1201 tacaagaaga gcctcgtcga ttatttcctg tgtcagaggg tcaaagacgt cacccagata
     1261 taaacgatga ctgacaaagt tttctactat atacccggag cgagaagtaa aaatccggga
     1321 ggcaatccta actgcttcac gaccactcat ccgcacaata ccaattgctc ccaccccgag
     1381 aggagtggcc ggagctacaa tagtatcatt tactaacaga ccttctattt ccttcactgc
     1441 gcttactact tctttccttt ttcaagtcaa taaccacccg ccttcctggt tcttttccta
     1501 tgctgtaagt ggttacctcc gggtaatctt taagagaagt atggataatt ctccgctccc
     1561 gggcattcat aggttccaac tttaatctcc ttttttcttt cattaccttt ttagctgatt
     1621 tttccgccag cttcttcagt gctttttctc gtttttctcg ataaccagaa atatccagct
     1681 gcaaaattac atcgttaaaa ccttttttgg acatgaccat tcgcagcagg gtttccaaag
     1741 cctccagagt ttctccgccc ttaccgataa gcatcccagc atctttccct tctaccaaga
     1801 ggaaaagaaa accgtctctt tcttgaacct ggaagtcaac ctccatctcc attttgttca
     1861 tcagttcctt aaaaaacttc tctagttctt ctcccgcttg aaggagataa gctggagaag
     1921 agggagcttt ctctcctacc tcttttctgg gggcttcctc tctggaaatt tcttctatcc
     1981 acacccgaac ctttactact cgaggggtaa aggcgctcaa aaaaccgcga ttttctgaaa
     2041 taatttcata atttaaactt tctggcgaca catcaaaata acgagctgct cgctccaatg
     2101 cttcttccaa gctttttcct gaaacctcaa cgcttcttgg cactcgtttt tcctcctttt
     2161 ttaactgtag aacctgtcaa agagggactt tctaaagaaa tagcgggttc aacctccgca
     2221 ttaatttccg gtttaaggga gagggccttg gccttttgga tttgattatt caatacatac
     2281 tgctgtaaaa tgtaaaacag gttagataca aaccaatata aaagcacccc ggcgggaaga
     2341 ttccagctga taaacaccaa aaacaccggc atgactacca tcataaccat actttgttgc
     2401 gcctgcgcct tatcagtagt aggaggcatg gatattttct gctgccagaa ggtagttatt
     2461 cccatcaaaa gaggaaggat aaaataaggg tctttttgag aaagactggg aagccagaga
     2521 aacccggctt gcccataatc ataagccatt aaaacccggt aaagcactac caatataggt
     2581 aattgaatta aaagaggaag gcatcccggt aagcaccctc ccaaaggatt tattcccttt
     2641 tctttgtaaa gcttcatcat ttcttcattc agtttttgac tgttgttacc atatttctcc
     2701 tgcaattttt tgatttccgg ctgcaactcc tgcatatctt cttgcatctt gcgagtggag
     2761 agagtttgct tgtgaattaa aggagaaaga atcaatctga tcaataaggt aaggagaatt
     2821 atagaaatac catagtcgcc ggtccaatta tagaaaaaac gaagcaggta ttccatagct
     2881 tgagccagta tatctaaaag ctgcgaaaac attgattaac caccttttct ttttatttta
     2941 ccggatcata accacctgga aagtaaggat tacaacgcat aagccttcga agagctaaaa
     3001 agccaccttt ccagagacca tatttacgga gagcttgtcg ggcatattct gaacaggtag
     3061 gctcaaagcg gcaggaaggg ggaaagagag gagaaatata tttctgataa aaaagcaaga
     3121 ctttatctaa accgcttact acaaaattac ttaatttcac tttctgtctt tactccacaa
     3181 atctctactt cattcagcac ttttcccata gtttcaacca ggtctttaaa accttctttc
     3241 agtaattttt cattactcat aaaaacaaag tccaccggtt tatctctcca ttgaggataa
     3301 tagcaaacac gaaaggcttc ccgcaaacgc ctccgtactc gattcctatc taccgctttc
     3361 tttcctttcc ccacaaaagc aacccgcaag cttccctcat tcttttgtaa ataccatagt
     3421 ttaatactat ccaccttcac tctttttcct tgagagaaca accgggagaa atcttttttc
     3481 tcactaagtc cctccattta tcaaaccgtg aggcgcttac gtcctttcct ccttcttcta
     3541 gagagtattt ctctccctcc ctgggttttc atccgagccc gaaagccatg agttcttttc
     3601 atcggtatcc gatgaggctg ataagtcctt ttcatcgaag cttctccctt ctttactgct
     3661 ttaaaaaaga gtaatttacc acggatatca ttatactctc aaccaccccg gaggtcaatc
     3721 agaagaaaaa gaggaccaat cacttttata aattataaaa aggatagaac taacgctgaa
     3781 agtgatttta aaccaggttt ctctcccatt ccactactaa gatgaaaaag cggtgcacaa
     3841 ctaaagccag aaaagcagag ataagaaagt aacatccttc atggttatca acttctcaag
     3901 cttctcttgt gaaagaagaa attttgtgct aaattccctc cagaaataat ttaccagggt
     3961 tggtgctatg aaaagaaaat taagagtggc tgtcatctgt ggtggaaaat cggtagagca
     4021 cgagatttct cttcaatcag caaaaaatgt tatagaagct atggatgggg ggaaatatga
     4081 tattgttctt ataggcattg acaaggaggg ctactggcat cttaaggcct taactgagct
     4141 tcaagaagag atgaaaggtt ctcttctacc tcaccttacc tctcaaggag atgaagtaat
     4201 tttcgttcct ggaggcgcag gacaactggt taatctctcc actggagaaa ggctacaacc
     4261 ggtggatgtg gtttttccag ttcttcatgg accgttcggc gaagatggtg ccatccaggg
     4321 attatttaaa atagcgggtg tcccttttgt gggagcagga gtgctgggtt cagctattgc
     4381 tatggacaaa gaagtaagta aaaaattgct gagagaagct ggaattccta cccccaaatt
     4441 tttatccttt gtttgccaac aaaaagagga aatcaaattc tacgaagtag ctaactactt
     4501 aggagtacct ttctttgtaa aaccggccag ccttggctca tccgtgggta taagtaaggt
     4561 taaaaaagag gaagagcttc gccctgcttt agaagaagct ttccgctacg atggtaaaat
     4621 cttaattgag gaatacattg agggacgaga aattgaatgt gctgttctag gaaacgaaaa
     4681 acctcgtgct tcttgccctg gagaaatcat c
//
LOCUS       DAOSVE010000111         4710 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_7872_length_4710_cov_74.312352, whole genome shotgun sequence.
ACCESSION   DAOSVE010000111 DAOSVE010000000
VERSION     DAOSVE010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7872_length_4710_cov_74.312352"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..253
                     /locus_tag="PK016_07825"
     CDS             95..253
                     /locus_tag="PK016_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL03003.1"
                     /translation="MGCYRIKTIMLGKARRCPVALWHGKNKFFCTVQEHAIMILVTDC
                     KFNIGRDV"
     gene            286..1740
                     /locus_tag="PK016_07830"
     CDS             286..1740
                     /locus_tag="PK016_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002680394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HXL03004.1"
                     /translation="MPKVNWTLDNLDEILSTDFTPQQSAQIKTLFFMRLAERAHRFYG
                     GKMQTVPKAGVFGFNWFNVWYTPGVSKISTTIRDNNDESFELSNRGNSVAVVSDSSRV
                     LGDGDCTPPGGLGVMEGKAFLMKYLGGIDATALCIDSRDEKGNHRAEKIIDFVKMVEP
                     SFGAVNLEDISQPNCYQVLDTLREECNIPVWHDDAQGTACVTLAGLINALKLADKKIE
                     EVKIVLLGAGASNTTIARFIKLAGGNLENMVVFDSRGALHRDRRDIESDPRYYKKWEL
                     SLKTNPQKITDIEEAMRGADVLIALSTPGPDTVKPDWIRLMADKSIVFACANPVPEIY
                     PYAAREVGAFIVATGRGDFPNQVNNSVGFPGILKGALLVRAKKISDGMAIAAAYSLAN
                     YAEKRGIHPDNIIPSMDEVGVFPHEARDVAQQAIKENLARVEMSEEEAFSQAEKDIKY
                     ARELVQVMMSEGFISAPPESLLQEALDWAIQQIH"
     gene            2121..2387
                     /locus_tag="PK016_07835"
     CDS             2121..2387
                     /locus_tag="PK016_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXL03005.1"
                     /translation="MVEQIFKALSSPWRVEILKRIAEVPLCQCELEKTMAIDKTTISR
                     HVRELVLTGLVEIEQKGVMKILHIKDERVMEIIALAEDMVEEKV"
     gene            2388..3401
                     /locus_tag="PK016_07840"
     CDS             2388..3401
                     /locus_tag="PK016_07840"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HXL03006.1"
                     /translation="MWQTALQEGLRTIVAYLNPWHLLLGIMPAFLISGAIATLMDREN
                     ILRFFGPGANKWIAITIASIAGAILAVCSCSILPMFTSMYKIGAGIGPATSFLYSGPA
                     INTAAIFISAAILGKIGWARSLSAVIVAFVIGLIMDFGFGKAQLRQAKEKKMEATSSL
                     GVPWKGLLVIFLLVAIYMVGLNLFKGILLVVAFLLGYILFSKEECKSWHQESWSLFKQ
                     IFPILLIGVFIASGLKAFVPPSLIVKLFGRESFVSNMFSSVIGAFLYFSTLTEVPITQ
                     SFIEMGMSSGSAIAFLLAGPALSLPSMITINRVMGWKRGLSYIGLVILFSAISGSVYE
                     FIF"
     gene            3419..3652
                     /locus_tag="PK016_07845"
     CDS             3419..3652
                     /locus_tag="PK016_07845"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HXL03007.1"
                     /translation="MKIQVLGAGCKKCNLLYQNLKSFVEKNNLDAEVEYSDDLDKLME
                     AGVIMPPAVFVDGVKKSEGKIPTEAQFKEWFQV"
     gene            3773..4492
                     /locus_tag="PK016_07850"
     CDS             3773..4492
                     /locus_tag="PK016_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HXL03008.1"
                     /translation="MKIAVFSDIHSNIEAFRAVMQDIEAQKPDHLICLGDLVGYAPFP
                     NEVIAAVHSLDIPVIMGNYDQGVGNDLNDCGCAYKTDEERALGNISLQWTKKVTTPEN
                     KIYLKNLLPRYQMTVGDYQILFVHGSPRRINEYLFPERPDESLLHVMMNETANILFFG
                     HTHRPFSRMVQNIWFINDGSVGHPKDGDWRAGWVLLTIEGNLPLEIDFRRCEYDLESL
                     KVAYQSSDLPQKFYYDLLPTK"
BASE COUNT         1353 a          820 c         1097 g         1440 t
ORIGIN      
        1 cagttttgcg ggatactata aattgactat agaaagaagt aatcttccta tcacttcctt
       61 ttgaaaaaca aagaacaaaa ctgtggcagg ttttatggga tgctatagaa ttaaaacaat
      121 aatgttggga aaagcacgcc ggtgtcctgt ggcactgtgg cacgggaaaa ataaattttt
      181 ctgtacagta caagaacacg ctataatgat tcttgtaact gactgtaaat ttaatattgg
      241 ccgggatgtc tgaagatagg attattaaat taaggaggaa atgatatgcc taaagtaaac
      301 tggaccctgg ataatttaga tgaaattctt tctaccgatt ttactcccca acaatcagca
      361 cagattaaaa ctctcttttt tatgaggtta gctgagcgag cccatcgatt ttatggaggt
      421 aagatgcaaa ctgtacccaa agcaggagtt tttggtttta actggtttaa tgtctggtat
      481 actccagggg tttctaagat ttctaccact atcagagata ataacgatga atcttttgaa
      541 ctttctaacc ggggaaattc agtagcagtg gtgagtgatt cttcccgggt tttgggagat
      601 ggagattgta ctccccctgg ggggctggga gttatggagg gtaaagcttt cttgatgaag
      661 tatttaggag gaattgatgc tactgcttta tgtattgata gtagagacga gaaaggaaac
      721 catagagcag aaaaaatcat tgattttgtg aaaatggtag agcccagttt tggagcagtt
      781 aatctggagg atatatctca acccaattgt taccaggtgc tagatacttt gcgggaagaa
      841 tgtaatatcc cggtctggca tgatgatgct caaggtactg cctgtgttac tctggctggc
      901 ttaatcaatg ctctgaagtt ggcagataag aaaatagagg aagttaagat agtgctttta
      961 ggagctggag cttccaatac cactatagct cgttttatta agttggcagg aggtaatcta
     1021 gagaatatgg tggtatttga ctctcgagga gctctgcatc gggatagaag agatatagaa
     1081 tctgaccctc gttattacaa gaagtgggag ttgtctctca agactaatcc ccaaaagatt
     1141 accgatatag aagaggctat gagaggagct gatgttttga ttgctttatc tactcctggc
     1201 cctgatacgg taaagcctga ttggataaga ctgatggcag ataaatccat agtttttgcc
     1261 tgtgctaatc cagttcccga gatttatcct tatgcagctc gggaagtggg agctttcatt
     1321 gtggctaccg gtagaggtga tttccccaat caggtgaaca actcagtagg tttcccgggg
     1381 attttgaaag gagcattact tgtcagagct aaaaaaatat ctgatgggat ggccattgct
     1441 gccgcttatt ctttagctaa ctatgctgaa aaaagaggta tacatcctga taatatcatc
     1501 cccagtatgg atgaggtagg agttttccct catgaagcaa gagatgtagc tcaacaggct
     1561 attaaagaaa acctggctcg ggtggaaatg tctgaagaag aggccttttc tcaggcagag
     1621 aaagacataa aatatgctcg ggagctggtg caggtgatga tgagcgaagg atttatttct
     1681 gcacctccgg agagtttgct tcaagaagct ctggattggg ctatacagca aattcactga
     1741 tgagtaaaga gggaagtcaa tagcaattga ggttgtttga aaaatacctc tctatactca
     1801 atcaccatta cccagctata tcatttatac tggtaaagga attttctaag cttttatagt
     1861 gataactatt aaatgaagga tgtgatagta tgagaaggaa tgccattcct tttatagtta
     1921 accgcaaaat tcattataaa gactatgagg gggtaaatgt agtatttctc agcttgtcct
     1981 ctggaatata ggaaatattc ttgttatctt ctttgcagcc ctgaggtata gaaagaattt
     2041 tcaaacagcc tcaaaagata ttgacaatca tcttgcgttg ttgtaataca ttacaataat
     2101 acaataaata ggtgatggat atggttgagc agatttttaa ggctttgtct tctccttggc
     2161 gtgttgagat tttgaagagg atagcagaag tgcctctctg tcaatgtgaa ttagaaaaaa
     2221 ctatggctat cgacaaaacc actatttccc gccacgtgcg ggaattggtt ctgacaggtc
     2281 ttgtggagat tgagcaaaag ggagttatga agattttgca tatcaaagat gaacgagtta
     2341 tggaaatcat tgctctagct gaagatatgg tagaggaaaa agtataaatg tggcaaaccg
     2401 ctttacagga aggacttcgc accatcgtag cctatcttaa cccttggcat cttttgttgg
     2461 ggattatgcc agcttttctt atctcaggag caattgccac tctaatggac cgggagaata
     2521 tcttgcgctt ttttggcccg ggagccaaca agtggattgc catcactatc gcctccattg
     2581 ccggagcaat tcttgccgtt tgttcctgtt ccatacttcc tatgtttact tctatgtata
     2641 aaataggagc aggaattggt ccggctacca gttttctcta ttctgggccg gcaattaata
     2701 ctgcagctat ctttatctct gctgctatac tggggaaaat cggctgggct cggtcgcttt
     2761 ctgcggttat agtggctttt gtgattgggc tgataatgga ctttggtttt ggtaaagccc
     2821 aactgcgcca ggcgaaggaa aagaaaatgg aagccacttc ctctttggga gttccctgga
     2881 aggggctatt ggtcattttt cttctcgtcg caatctatat ggttgggcta aatctattta
     2941 aaggaatact actcgtggta gcttttcttt tggggtatat tttatttagc aaagaagagt
     3001 gtaagagctg gcatcaagaa tcctggagtt tgttcaaaca aatttttccc atattattga
     3061 taggagtgtt cattgcttca gggctgaagg cttttgttcc tccttctctc atcgtaaaac
     3121 tttttggaag agagagcttt gtttccaata tgttttcctc ggttatcggt gcatttttgt
     3181 actttagtac tcttaccgaa gttcctataa cccaaagctt tattgagatg ggaatgagct
     3241 ctggctcagc tattgccttt cttttagctg gtccggcgtt aagcctcccc agtatgatta
     3301 ccatcaaccg ggttatggga tggaaaagag ggttaagtta tattggattg gtaattttat
     3361 tttctgccat ttctggttcg gtatatgaat ttatatttta gcttaggagg tgcaaataat
     3421 gaaaattcaa gtcttggggg caggatgcaa aaaatgcaat ttgctctacc aaaatcttaa
     3481 atcatttgtg gaaaagaata atctggatgc tgaggtggag tatagtgatg acttagataa
     3541 actgatggaa gccggggtca tcatgcctcc ggcagtgttt gtcgacggag taaagaaaag
     3601 tgaagggaaa attcccaccg aagctcaatt taaagagtgg tttcaggtat agtaaaagcc
     3661 gccgcagtgg gggagattcc ccactgtatt taattaactc tatgccaaaa aatagagagt
     3721 aaagctggtt agttttttgg ggtcataggg ggaaaacaag gagaaagagg ttatgaaaat
     3781 tgccgtcttt tctgacatcc attctaacat agaggcgttt agggctgtta tgcaagatat
     3841 cgaagctcaa aaaccagacc atctcatatg tctgggagat ttagtggggt atgctccttt
     3901 tcccaacgag gttatagcgg cagtgcattc tctcgatatc ccggtaatta tgggaaatta
     3961 tgaccaggga gtgggaaatg acctaaacga ctgtggttgc gcttataaga ctgacgaaga
     4021 aagagcgctg ggtaacattt ctctgcaatg gactaaaaag gtgactactc cggagaataa
     4081 aatctattta aaaaatcttc ttcctcgata tcagatgact gtcggagatt atcagatact
     4141 ctttgttcac ggtagtcctc gtcggattaa cgaatatctt tttcccgaac gccccgatga
     4201 gagtctcctt catgtgatga tgaatgagac tgctaatatt cttttctttg gccatactca
     4261 tcgacccttt tcccgtatgg ttcagaatat ctggtttatt aacgatggga gtgtgggaca
     4321 ccccaaagat ggagactgga gagcaggatg ggtgctattg actatagagg ggaaccttcc
     4381 tctcgagatt gattttagac gttgtgagta tgatttggaa tctcttaagg tagcttatca
     4441 aagctctgat cttcctcaaa agttttacta tgaccttctt cccactaaat gatagagaga
     4501 ctatggtggc gggaaattca ttataatcaa tctcttaaac cttaaaaggc aagctgaata
     4561 ggctaaaaac tggagattta attgagttcg gtaaacttcc tattactcct tttttgcctt
     4621 gttaccaatt ttcttctctt tctatataac agactaaaaa gaaagagcta tcttccggaa
     4681 gtaaagccta tcttccctta gagcataaac
//
LOCUS       DAOSVE010000112         4596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_8076_length_4596_cov_74.106805, whole genome shotgun sequence.
ACCESSION   DAOSVE010000112 DAOSVE010000000
VERSION     DAOSVE010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8076_length_4596_cov_74.106805"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="PK016_07855"
     CDS             complement(<1..1052)
                     /locus_tag="PK016_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXL03009.1"
                     /translation="MRWLFFPDLRVLELNFVQWIIIFFCFYGIYQTIISFRGFWSAKP
                     LPSASYLRRFAVLIPAHDEEKVIGSLLDSLNKQTYPRDLFDIYVACDACSDATPEIVR
                     EHGGVALLRNDPLHPGKTQNIRWALNNIPLDKYSAIVIFDADNLAHPDFLSRMNDYLE
                     AHPEAEAVQGYLDTKNLSDSWLTKVYALAYWYTNRFWQLARANWGLSATLGGTGLLIT
                     VPCIKRLGWDLQSLTEDLEFSTKLILSGGKVHWNEWAITYDEKALSYLASHRQRLRWM
                     QGHYWVGWHYGPKLLIQFFKTLRFQYLDYFIYLLTPLTIILSFVLTIFEVLRWVILAP
                     LPGAFFFWYWWIPFVF"
     gene            complement(1092..1619)
                     /locus_tag="PK016_07860"
     CDS             complement(1092..1619)
                     /locus_tag="PK016_07860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXL03010.1"
                     /translation="MVWGIGLFLIVVALLFFFWFFPVFFNGIPWQPTDMKRVRRMLEM
                     ANVKPGEVVYDLGCGDGRIIICAAQEFGARAVGVELNPWLYFLALLKVWTKGLSSRVR
                     VIFGNLHQVDLKEADVVTIFLYSHVNELLQEKFQRELKPGARIVSYVWVLKNWEPVAI
                     DSKYDLYLYVKDSVN"
     gene            complement(1604..2470)
                     /gene="lipA"
                     /locus_tag="PK016_07865"
     CDS             complement(1604..2470)
                     /gene="lipA"
                     /locus_tag="PK016_07865"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HXL03011.1"
                     /translation="MSSLPSWIKKRISLGEEGEETQKLLQSLSLHTVCESAHCPNRGE
                     CFMKKVATFLIMGNICTRRCRFCAIDKGTPQPLDPEEPKRVAEASQVLGLRHVVVTSV
                     TRDDLPDGGASHFAATIREIRRALPFSTVEVLTPDFQGREEDLQVIVKEKPEVFNHNM
                     ETVPRLYPSVRPLASYKRSLTLLKRVKEISPSIITKSGLMVGLGERKEEVVEVLEDLR
                     EVGCDVITIGQYLRPSPRHLEVKEYVSPQVFSWYEDVARSLGFRGVASGPFVRSSYLA
                     EKFVGEKLWSGA"
     gene            complement(2467..3180)
                     /gene="lipB"
                     /locus_tag="PK016_07870"
     CDS             complement(2467..3180)
                     /gene="lipB"
                     /locus_tag="PK016_07870"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HXL03012.1"
                     /translation="MEKLWFVRGGQVDYSFAHRLQLNLREKVERENIPGFFLLMEHPP
                     VFTLGRGASRSHLLVDESILEKEGIKVYEIERGGDITYHGSGQIVGYPIINLDFWERD
                     VHLFLRALEEVLIHFLKRFEVEGFRLPPYTGVWVNKEIPQKIAAIGVAVKKWITYHGF
                     ALNISTFLPHFNYIIPCGISDKGVTSLERITGNSYSLEEREEMEELLAQDLGEVLGFK
                     MEEISRDDFFLGRERRLEI"
     gene            complement(3213..3306)
                     /locus_tag="PK016_07875"
     tRNA            complement(3213..3306)
                     /locus_tag="PK016_07875"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3270..3272),aa:Ser,seq:gct)
     gene            complement(3373..4512)
                     /locus_tag="PK016_07880"
     CDS             complement(3373..4512)
                     /locus_tag="PK016_07880"
                     /inference="COORDINATES: protein motif:HMM:NF027610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4932 domain-containing protein"
                     /protein_id="HXL03013.1"
                     /translation="MSAGKLSKSKWLYFFIGVLAILIILFIGFAPRAHYTRAYIEENR
                     GKVIVEIPEVFELANIIIAIANHELTNNPYSIFQQGEYYERVLHHFLPFKNHPLISEI
                     REEFSGEDRGSKYYRFRDNSVCYVFKGDSIVNAGLYSVVFSPDLFKEYINLVEDFARV
                     SGFREFYDANQSYYQEQIQKYKERVPVRKMWDWLENNFPQQYDCYKVIFSPLIQGMHH
                     TERFETRNFKEIVMFICGPDAFHVRLPEKIEEGLLPRAVFTEIDHNYVNPITKKYSDK
                     VEKAFSDIQKWNIQSQYWYPTPVITFNEYMTWAVFTLYAYDTYEKEDFEIINQFTVNQ
                     MVNSRGFVLFEQFNNQLLELYVNREEGETIPDLYPEILEWVENYP"
BASE COUNT         1467 a         1034 c          795 g         1300 t
ORIGIN      
        1 aaaaaaacaa aaggtatcca ccaataccaa aaaaagaacg caccggggag aggagcaagt
       61 atcacccaac gcaatacttc gaaaatggtc aaaacaaagc ttaaaataat ggtaagaggg
      121 gtcaaaagat aaatgaaata atccagatat tggaaacgaa gagttttgaa gaattgaatt
      181 agtaatttag gtccataatg ccatcctacc cagtaatgac cttgcatcca tcgtaacctc
      241 tggcgatgag aagccaaata agaaagagct ttctcatcgt aagttattgc ccattcattc
      301 caatgcactt ttcccccaga aagaattagc ttggtactga attccagatc ctcggttaag
      361 ctctggaggt cccaacctaa acgtttaata cagggaacag taatgagtag acctgttcct
      421 cccagcgtag ctgataaccc ccagttagct cgggccaatt gccagaaacg attggtatac
      481 cagtaagcta aagcataaac tttagtaagc caggaatcag aaagattttt agtgtccagg
      541 taaccctgta ctgcttccgc ttcaggatga gcttctaaat aatcattcat tctagagagg
      601 aaatcaggat gagccagatt atcagcatca aatataacta ttgcgctata tttatcaagg
      661 ggaatattgt tcaatgccca gcgaatattt tgagttttcc ccggatggag agggtcgtta
      721 cgcaacaaag ccactccacc gtgttcccta acaatttctg gggtagcatc actacaagca
      781 tcgcaagcca cataaatatc aaataaatct cgggggtagg tttgtttatt aagactatct
      841 agcaaagaac cgataacttt ttcttcatca tgagcaggaa ttaaaaccgc aaaccgccgc
      901 aggtaagaag ctgaaggtaa gggttttgct gaccaaaaac cacgaaaact tataattgtc
      961 tggtatatcc cgtagaaaca aaaaaagata attatccact ggacgaaatt aagttctaat
     1021 actcgaaggt caggaaagaa taaccagcgc aatgtatcat ctcccgtttt atagctcaaa
     1081 agaaaaattt actaattaac agaatctttc acgtataaat aaaggtcata tttactatct
     1141 atagccaccg gttcccaatt ctttaaaacc cagacgtagg aaacaatgcg agctcccggc
     1201 tttaactccc gctgaaattt ttcctgcaaa agttcattta catgagaata caaaaatatg
     1261 gtgaccacgt ctgcttcttt taaatctacc tgatggagat taccaaaaat cactcttacc
     1321 cgagaagaaa gacctttagt ccaaaccttc aacaaggcta aaaaatagag ccaaggatta
     1381 agttccaccc ctactgctcg agcaccaaac tcttgtgcgg cacaaataat aattctcccg
     1441 tctccacaac ctaaatcata cactacctca ccaggtttga catttgccat ttctaacatt
     1501 cttctcactc tcttcatatc tgtaggctgc cagggaatac cattaaagaa aacagggaaa
     1561 aaccagaaga aaaataagag agctacaacg attaaaaata atcctatgcc ccagaccaca
     1621 gcttttcacc cacaaatttt tctgctaaat aagagctacg aacaaaagga ccactggcta
     1681 ctcctcgaaa acctaaagaa cgagctacgt cctcatacca ggaaaaaacc tgaggagaaa
     1741 cgtattcctt cacctctagg tgtcgaggag agggacgaag atactgacca atagtaatca
     1801 catcacatcc tacctctctt aaatcttcta atacttctac cacttcttct tttctttccc
     1861 ccaagcctac catcaaaccc gacttagtga taatagaagg agaaatctct tttaccctct
     1921 taaggagagt taatgacctc ttgtaagaag ccagaggtcg cactgagggg taaagacggg
     1981 gaacggtctc catattgtga ttaaaaactt ccggtttttc ttttactatt acttgcaaat
     2041 cttcttctct cccttgaaaa tcaggagtaa gaacttctac cgtagagaag ggcaaagccc
     2101 gccgtatttc tcgaatggta gcggcaaaat gagaagcacc cccatcaggg aggtcatctc
     2161 gagtaactga ggtaacaacc acatgtctca atcccagaac ctgtgaggct tctgctactc
     2221 tttttggttc ctcgggatcc aaaggttgag gagttccttt gtctatggca cagaaacggc
     2281 atctcctggt acagatgtta cccataatta aaaaagtagc taccttcttc atgaaacatt
     2341 ctccccgatt aggacaatga gcactttcac aaacggtatg gagagaaaga gactggagta
     2401 atttctgggt ttcttctcct tcttccccca gagaaattct cttctttatc caagagggta
     2461 aactgctcaa atttctaatc tcctttctct tccaagaaaa aaatcatctc tggagatttc
     2521 ttccattttg aatcccaaaa cttctcctaa gtcttgagcc aggagttcct ccatctcttc
     2581 tctttcttcc agagagtaag aattaccggt gatacgctca agagaagtta ctcccttatc
     2641 agatatacca cagggaatga tgtaattaaa atggggaaga aaagtggaaa tgttaagagc
     2701 aaaaccatga taggtaatcc atttcttcac cgctactcct atagcagcaa ttttctgagg
     2761 tatttcttta ttgacccata ccccggtata gggaggaaga cgaaaacctt ccacttcaaa
     2821 tcttttcaaa aaatggataa gaacctcttc caaagcacgt aaaaaaagat gaacgtctct
     2881 ttcccaaaaa tcaagattta ttatagggta gccaacaatc tgccctgaac catgataagt
     2941 gatgtctcct cctctttcta tctcatacac cttaattccc tctttttcta aaatgctttc
     3001 atccaccagg aggtgacttc gagaggctcc ccttcccagg gtaaaaaccg gagggtgttc
     3061 catcaaaagg aaaaaaccag gaatattttc tctttctact ttttctcgga gatttaactg
     3121 cagcctgtga gcaaacgaat aatccacttg tcctccccgg acaaaccaga gtttttccac
     3181 ttatctcatc acgactttgc caaacataaa actggcggag agggtggggt tcgaacccac
     3241 gaggcgagtt ttaactcgcc taatcgctta gcaggcgact gccttcaacc actcggccac
     3301 ctctccactt ttcgttagta tatcacattt ttttattttc tcaactttca cacaaagctc
     3361 tttctgtcca cgctaaggat aattttcaac ccattccaaa atttcaggat acaaatcagg
     3421 tattgtttct ccttcttccc gatttacata gagctctaac agttggttat taaactgttc
     3481 aaaaagtaca aatcctctgg agttgaccat ttgattcact gtgaattggt ttattatttc
     3541 aaaatcttct ttttcatagg tatcataggc atacaaagta aaaactgccc aggtcatgta
     3601 ctcgttgaaa gttataaccg gtgtgggata ccaatattga ctctgtatgt tccatttttg
     3661 aatatcagaa aaagccttct ccactttatc tgagtacttc ttggttatgg gattgacgta
     3721 gttatgatct atttcagtga agaccgctcg aggtaaaagt ccctcttcga ttttctcagg
     3781 aagtctgaca tgaaaagcat ctggtccaca gatgaacatc acaatttctt taaagtttct
     3841 tgtttcaaag cgttctgtgt ggtgcatgcc ttgaatcagg ggtgaaaaga taactttata
     3901 gcaatcatat tgctgaggaa aattgttctc caaccagtcc cacatctttc tcaccgggac
     3961 tctttcttta tatttttgaa tttgctcttg ataatatgac tgatttgcgt cataaaactc
     4021 tctgaaacca gaaactcggg caaaatcttc cacaagatta atgtattctt tgaataaatc
     4081 aggtgaaaat actacagaat acagtcctgc gttgactatt gaatctcctt tgaaaacata
     4141 gcaaacacta ttgtccctaa aacgataata cttagagcct cgatcttctc ctgaaaattc
     4201 ctctctaatt tcagagatta aaggatgatt cttaaaggga agaaaatggt gtaaaactct
     4261 ttcgtagtat tcaccctgct gaaaaatgct atagggatta ttcgtcagtt catgattagc
     4321 aatggctatt atgatatttg ctaactcaaa cacttcaggg atttctacta tcactttacc
     4381 cctgttttcc tcaatgtagg ctctggtgta atgcgcccga ggagcaaagc cgataaacaa
     4441 tataattaat atagctaata caccaataaa gaaataaagc catttacttt tacttaattt
     4501 tccggcagac ataatttaaa tctccttttc tatagtatcc cgcaagttca acctgcactt
     4561 catgacgagt gagctgtcat acataaagcc ataata
//
LOCUS       DAOSVE010000113         4520 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_8235_length_4520_cov_70.378499, whole genome shotgun sequence.
ACCESSION   DAOSVE010000113 DAOSVE010000000
VERSION     DAOSVE010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8235_length_4520_cov_70.378499"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..840)
                     /gene="radC"
                     /locus_tag="PK016_07885"
     CDS             complement(7..840)
                     /gene="radC"
                     /locus_tag="PK016_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HXL03014.1"
                     /translation="MKCSKGGILTQEKKKEIIEHLNIKTSSECATLRETIKKQVAERR
                     TTRPIKEWIKEERPREMLIRLGAENLPLSKLLAIILRTGKNGSSAEELAQNLLNHFGS
                     LRAIDAAPISELSKIEGIGPAKAAQIKAALELGKRFLQERASTRKKLTKPDDVINYVC
                     EYYAPYLRDQEKEFFNVILLDIKNKPVDNIEISKGSVSATIVDPKEIVKMASLKSASS
                     VILVHNHPSGETTPSQDDIELTNRIVSACELLGIKVLDHIIIGKNMEDFYSFAKEGLI
                     G"
     gene            1382..3130
                     /locus_tag="PK016_07890"
     CDS             1382..3130
                     /locus_tag="PK016_07890"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HXL03015.1"
                     /translation="MKKKQLKLIFFLLVIFSFFSISVQAQDVVTWRDFSQELTRLLQL
                     PSGRIPDFGEGEKPISRLEAAKMVIQALSYQDLLSFVDTKDVPFSDLANISSEEQKWM
                     VLASTLEPPLYQGDSGGRLRPKDPLTRGEFSQLKERISHYARGEVKWQREKAINQYLT
                     LLIKKEGFSPSPSIETPEAKGEVMLQVGAFQDQERAERTSTWLKELGYTVQMKKEGDL
                     FKVRVGPYSREEAPQVSARLQKQGFVSYQVAVETTPVTTAVAPTTPLGPFFSLGLLFD
                     PYSPLRLEIALAQDKVLEREKTSEIARRKGAVFAVNAGFFAQDGDPLGTLMIDGRILS
                     EPQEGWYNVGITEDNQLLFGELKLRAQAQAENGTTYSIAGINRRNNGKELVFYDHFLG
                     EKTPQQEGIESIIVRGRVIETKKSEGGTVIPTDGFILQGKGEASQWMWQNLIPGSAVK
                     VKMSIYPQDEGWEKWKEVKYILSSGPLLFRNGQPGPFGNFNEKIVTEKHPRTIIAQTE
                     EGKILFLVVDGRNPAHSVGLTIPELVEELKNYRVVNALNLDGGGSTTFYLQGQILNQP
                     SDLLGERKVSTAFIIR"
     gene            complement(3132..3794)
                     /locus_tag="PK016_07895"
     CDS             complement(3132..3794)
                     /locus_tag="PK016_07895"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXL03016.1"
                     /translation="MIKAIIFDIDGTITDTNPLFLRSISQTFREFTGNNKPESFFIFT
                     LGIPSPETLKALSIPPEKGKAFVVRWQKLIQEGMREVRLFPGIREIIEELHQEGIKMA
                     LVTSKIRSEMSYQFDQFMINHFFETIICAEDSPRPKPYPDPLLLALERLGVKNNESIF
                     VGDSIYDIQAAKGASIPFALASWGALELEKVLEFCPDFVLEEPEDIGEIVRGKRGLTP
                     VV"
     gene            4318..4475
                     /gene="ssrS"
                     /locus_tag="PK016_07900"
     ncRNA           4318..4475
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PK016_07900"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
BASE COUNT         1319 a          910 c          919 g         1372 t
ORIGIN      
        1 tctgattcac cctatcaatc cttcttttgc gaaactgtaa aagtcctcca tgtttttgcc
       61 gatgattata tggtctaata cttttatacc caggagctca cacgccgata ctatgcgatt
      121 ggttaattct atatcgtcct gggagggtgt agtttctccc gaaggatgat tgtgaaccag
      181 aatgacagaa gatgcagatt tcaacgatgc cattttgaca atttctttgg ggtcaacaat
      241 agtagcactt acactgcctt tgcttatttc aatattatca acaggtttat ttttaatgtc
      301 caggagaatt acattaaaaa attccttttc ttggtccctc aagtaaggag cgtaatattc
      361 gcaaacatag ttaattacat catctggctt ggttaatttc ttcctggttg atgctctctc
      421 ctgtaaaaat cttttaccca gttccagtgc tgccttaatt tgagctgctt tagcagggcc
      481 aatcccttca atcttagata gctcagaaat cggtgctgca tctatagctc tgagtgaccc
      541 aaaatggttg agcaaattct gggctaactc ttcagcgctc gagccatttt tccctgttct
      601 taagattatg gccagaagct tggataaggg caaattttcc gctcccagcc ttatcagcat
      661 ttctcgtggt ctttcttcct ttatccattc ttttataggg cgagttgtac ggcgttctgc
      721 tacttgcttt ttaattgtct ctcttaatgt tgcgcattcg ctactggttt tgatattcaa
      781 gtgttcaatg atttctttct ttttttcttg ggttaatatc cctcctttag aacatttcaa
      841 gaaaatagtc ccctttttgc tataaaaagg ataaatagca ttcgcaacca taaagtcgag
      901 gagctcccct cttgagctat tcatgctggt atccatctcc ttttaacttt cgtgtaaact
      961 tgaattgaat tccacttctt gcgccacaac aattttatgc tacgtacaaa acttctgcag
     1021 acttcagtat ctcagaggca attctatcga ttttcttcgc catgaacgag acctccgctc
     1081 actcaatctc ataaagtatg gaatcaataa gtttcgccct ggtatctgtc ttgctcaata
     1141 tttccacctt gattcctcat ccatatttta cacaatatct cataaacatg ccattttata
     1201 taaagactct ggatttgcga agtgtttaag tagagcgccg aaggtgaaga gctaacaagt
     1261 acattctgct aaacgaaatt gcctgagata actttttaaa acttcccgta acaggtttgt
     1321 actcttaaga gacaaagcgg ccttttcttt tttaagtatt tttgctaaac taattataga
     1381 aatgaaaaag aaacaactaa aactgatatt ctttttactg gtaatttttt cctttttttc
     1441 tatctctgtc caggctcagg atgttgtaac ctggagagat ttttctcaag aattgacccg
     1501 tcttcttcag ctcccctcag gaagaattcc cgattttgga gagggagaaa aacctatatc
     1561 tcgccttgag gcggcaaaaa tggtaattca agctctttcc taccaggatt tactctcttt
     1621 tgtagacacc aaagatgtcc ctttctctga tttagcgaac atctcttctg aagagcagaa
     1681 atggatggtt cttgcttcta ctttggagcc tcccctttat cagggagata gcggggggag
     1741 gttaagaccg aaagaccccc tcaccagagg ggaattttct caattgaaag aaagaatatc
     1801 tcattatgcc cggggagaag taaagtggca gagagagaaa gctatcaatc aataccttac
     1861 tctcttaatt aaaaaagaag ggttctctcc ttctcccagc atagaaaccc cggaggcgaa
     1921 aggagaagta atgcttcaag tgggagcatt tcaagaccag gagagggcag agagaacatc
     1981 tacctggctt aaagagctgg gatatacagt acagatgaaa aaagaaggag accttttcaa
     2041 agtaagagta ggtccttact ctagagaaga agctcctcag gtcagcgctc gtttgcaaaa
     2101 gcagggcttt gtatcttatc aagtggcggt agaaaccact ccggttacta ccgctgttgc
     2161 tcctactact cctctcgggc catttttcag cctgggttta ttgtttgacc cttattctcc
     2221 cttacgatta gaaatagcct tagctcaaga taaagtttta gagagagaaa aaactagcga
     2281 aattgctaga agaaaaggag cagttttcgc cgtaaatgca ggtttttttg cccaggatgg
     2341 tgacccctta ggaactttaa tgatagatgg aagaattctc agtgagcctc aggaaggttg
     2401 gtataatgtg ggcatcactg aagataatca actacttttt ggagaactaa agcttagagc
     2461 tcaggctcaa gcagaaaatg gcaccactta ctctattgct ggaattaacc gccgtaataa
     2521 tggaaaagaa ttagtttttt acgaccattt cttaggagaa aaaacccccc agcaagaagg
     2581 aatagaaagt attatagtca gaggaagggt tatagaaact aaaaagagcg aggggggaac
     2641 ggttattccc actgacggct ttattctgca aggcaaggga gaagccagcc aatggatgtg
     2701 gcaaaatctc atccctggct ctgcagttaa agttaaaatg tccatatacc cccaagatga
     2761 gggatgggaa aagtggaaag aggtaaaata cattttaagt tctggtcctc ttcttttccg
     2821 gaatgggcag ccaggaccat tcggtaattt caatgaaaaa atagtaactg aaaaacatcc
     2881 tcgcactatc attgctcaaa ccgaagaggg taaaatcctt tttctggtag tggatgggag
     2941 aaaccccgct cacagcgtag gattaactat tcctgaatta gtagaagaat taaaaaatta
     3001 ccgggtggtt aacgcgctta atttagatgg cggaggttct actacttttt atcttcaggg
     3061 tcaaattctt aatcaaccat ctgaccttct gggggaaagg aaagtttcca ctgctttcat
     3121 catccgttaa ttcaaactac cggggttaaa cctcgctttc ctcttactat ctccccaata
     3181 tcctctggtt cttctaatac aaaatcggga caaaactcca gaactttttc caactccagc
     3241 gctccccagg aagccagagc aaagggaatt gaagctcctt tagctgcctg aatgtcgtaa
     3301 atactgtcac ctacaaaaat agactcgttg ttcttaaccc ccaatctctc cagggctaaa
     3361 aggagagggt caggataagg tttaggacga ggggaatctt cagcacagat tatagtttca
     3421 aaaaaatggt ttatcatgaa ttggtcaaat tgataagaca tttcagagcg aattttagaa
     3481 gttaccagtg ccatttttat cccttcctgg tgcaattctt ctattatttc tctaatgcct
     3541 ggaaaaagtc gcacttctct catgccttcc tgaattaatt tttgccagcg aaccacaaat
     3601 gcttttcctt tttcaggagg aatgcttaaa gcttttaatg tctcagggct gggaatacct
     3661 aaagtaaaaa taaagaaact ctctggctta ttatttcctg taaattcccg aaatgtttga
     3721 gagatacttc gcaaaaataa cgggttagta tcagtaatag ttccatctat atcaaaaata
     3781 attgctttaa tcaatttatc actctccgat tctttagtta ttataatagg gatgtcaacg
     3841 gaggaagaga aatttaatct ctacggggtt tcagatttca atagatgggt tccaacgaat
     3901 taataatttg aagatattgt gaatgtaaac taaaaagtca tagaggcaga gacacgggat
     3961 ttagagagtc tataaaactt taagaaaatc gccagtagaa attctccaga tagaaagggg
     4021 agtgaagcct ctcatacttt ctgtctctct aaagatagtt cttaagtaag ctgaactaat
     4081 tgattccctt cttttcacgg gattattgcg tgactgatac caagattaag cttatgaggt
     4141 gagaaacgtt tactctcccc ggtatctatt gtgtgcccaa aattccgaaa gaatctcaag
     4201 ctaaatggat ggtgattgga aatattacct cccataggtt agcgctatcc tcatcaagtc
     4261 tttggttgaa aagagtacca tcttatgcta ttatttaagt gggagtttga agtggatatg
     4321 gccctgcttt gttggtgatg ccagagaagg ctttgaacca acacccttgt cttgggagcc
     4381 tgttcgctag tttgagctgt ggctcagctg ggagggcacc cacctttttg aggacgagga
     4441 tcaaagcggt tggcacacgg caaagtgggg ccatttagtg taaaggaaat ggaggaagga
     4501 gttgtttggt tgtggaaaat
//
LOCUS       DAOSVE010000114         4496 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_8297_length_4496_cov_68.054943, whole genome shotgun sequence.
ACCESSION   DAOSVE010000114 DAOSVE010000000
VERSION     DAOSVE010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8297_length_4496_cov_68.054943"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(755..1921)
                     /locus_tag="PK016_07905"
     CDS             complement(755..1921)
                     /locus_tag="PK016_07905"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014463773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HXL03017.1"
                     /translation="MRNFDYCNPTRVIFGKGETDRVGKECQKLGKKVLFIYGGESIKQ
                     NGLYDRVMRHLREEKLIVYELGGVKPNPRIDKVREGVAICKKEKIDLVLAVGGGSVLD
                     TGKVVAAGALYPGDPWDFFIDEHKISGKIPLVTVLTLAATGSEMNHNAVITNEETLEK
                     LGTHNPHLYPTLSILDPENTYTVPQDQTVNGIADMMAHVFEQYFHPLPETPLQDRWAE
                     GLMKTIIEYTPRVLQNPTDYDGRAVIMWCSTMALNHLLEEAGGGGEWTCHAIEHVLSA
                     VYDIPHGAGLAIIFPQWMKYVLDVIPHKFAQFAERVWEIPRAHKSDKELGLEGIERTR
                     EWFRAIGAPTTLKEVGIGEERIPEMARMAVKSGKLGVTKVLDEKDVEEILKMSL"
     gene            complement(1940..2617)
                     /locus_tag="PK016_07910"
     CDS             complement(1940..2617)
                     /locus_tag="PK016_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HXL03018.1"
                     /translation="MNLDALFTLNCGMYILCSGWEGKFNGLIVNALTQVTAFPPQLVV
                     SVNKESLTSTYIQKSGYFSVSVLEKNTPLPLIGLFGFHSGRNTDKLSQVKHFQGKTGM
                     PIITEHTVAYLEAKVVNQLDAGTHWVFLGEIVETDFMEKGGEPMTYSYYRDIKGGRVP
                     RTAATYHEEIKEKERYRCQVCGYIYDSSLGDPDSGIKPGTPFSDLPPQWVCPICGASK
                     DQFEKIQ"
     gene            complement(2633..4162)
                     /locus_tag="PK016_07915"
     CDS             complement(2633..4162)
                     /locus_tag="PK016_07915"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HXL03019.1"
                     /translation="MYLLGTDIGTQGTKTVMVNEKGEFIADAFQEYEVITPRPSWAEQ
                     WPEVWLEAVIKTISRCVEKSKVAPSEIAGLAISGLYGGSGVPVDEEIKPLYPCLIWMD
                     RRAREETQWVKNHVPQEKIFGITGNYVDSYFGFTKMMWIRNHEPEVWKKIYQFVTPKD
                     YVIYHLTGVLATDYSSAGNLGGVFDIRKRTWSEEMCEILGIPKRMLPEKILKSSDIVG
                     KLNARFAKETGLQEGTPVVSGGIDAPVAQFSAGVLAEGEHVAMAGTSMCWGTVHEGQY
                     LTPLLVSYPYVVYDDKLIYTFGGSATSGGLARWFRDQFGKYEKEVEKNTGVSAYTLLE
                     LQTQGIPPGSEGVVVLPYFMGERSPIWDPDARGTIFGLSLYHNRAHVYRAMLEAAAYS
                     LRHNMEEGIKAGLKLNPECWIVGGVARSSYWVQIFADITGFDMKRLSKDVEAPFGDAF
                     LAGLGTGVIDKPERIKEWVDFRPIIKVNQDNLHIYDKYYSIYRELYENTKETMKKLAD
                     I"
BASE COUNT         1156 a         1014 c          866 g         1460 t
ORIGIN      
        1 caaataatgg gttaagcctt ttctatgata ttgttctact cttctgaact gattttacta
       61 atataaaaaa actaaatggt gtatactttt tattgtgatt ttttattcaa tttttgcagc
      121 agttttctta tagctctttc ctttgttttt atcctcatcc ttccttagta ttaccgatat
      181 ctatctgaat ctggacacaa aaaagggcac agcccactga ggaagactct ttcccttcct
      241 cagtgagttg tgtttttcta tgtttctttg ccttttgggg tttataaggg ggcaaagctt
      301 tcttatagct ttcaccctca ccctaaccct ctcccttcga gggcgaggga aggaatgtgt
      361 gggattctcc cttcgagggc gagggaagga atgtgtgggg ttcttccatt gaaggcgagg
      421 gaagaaaaag aaaggggatt cataaggggg aattcttccc cctttgtacc gaggaagacg
      481 ctttccttcc tcggttgcat ttgtcttttt tgattttgta aatgttcttt gcttttctgg
      541 gggttataag ggggttttac ccactgaggg agacgttttc cctccctcag taatctttgc
      601 tttttttatg ttttttgggg gattataaag gggcacagcc cctttatagt tcggggttat
      661 gaggggaggg tcttttcccc cccctcatag tcattataat ggattttgcg gttaactata
      721 attagatttt ataaaattta tatattctct tttttcacag agacattttt aggatttctt
      781 ccacgtcctt ttcatctaaa acttttgtta cccctaattt tcctgatttg actgccatcc
      841 tggccatttc tggtattctc tcctcgccaa ttcctacttc ttttaaggtg gtgggagcgc
      901 cgattgctct aaaccactct cgagttcttt cgattccctc taaccccagt tctttatcgc
      961 ttttatgagc tcgaggaatt tcccagactc tttctgcaaa ctgagcaaat ttgtgaggga
     1021 taacgtccag gacatacttc atccactgag gaaaaattat agctaatccc gctccatggg
     1081 gaatatcata aactgcgctt aaaacatgct ctatagcgtg acaggtccac tctccaccac
     1141 ctcctgcttc ttctaagaga tggttgagag ccatagtgct gcaccacatg attactgctc
     1201 gtccatcgta atcggtgggg ttttgtaaaa cccggggagt gtattcaatg atggttttca
     1261 tcaatccttc tgcccaccta tcttgtaagg gggtttcggg aaggggatga aaatattgct
     1321 caaaaacatg agccatcata tcggcgatgc catttacagt ttggtcttga ggaacagtgt
     1381 aggtattttc agggtcgagg atagaaagag tggggtagag gtggggatta tgagttccga
     1441 gtttttccag agtttcttcg ttggtaataa cagcattgtg gttcatttct gaacccgtag
     1501 cagctaaggt taaaacagta accagaggta ttttccctga tattttgtgc tcatcgataa
     1561 aaaaatccca ggggtctcct ggataaaggg ctcctgcagc tacaactttt ccagtatcaa
     1621 gaacactccc tcctcctact gctaaaacca ggtcaatttt ctcctttttg caaattgcta
     1681 ctccttctct taccttatca atacgagggt taggtttaac tcctcctaac tcataaacaa
     1741 ttagcttttc ttctcgtaga tgcctcatta ctctatcgta taacccattt tgtttaatgc
     1801 tttctcctcc atatataaag aggacttttt tccctaactt ttggcattct ttccctaccc
     1861 ggtcagtttc tcctttacca aaaattactc tggtggggtt gcagtaatca aaatttctca
     1921 aatttacgcc tcctcaaatt tactgaattt tttcaaactg gtctttgctt gctccacaga
     1981 tggggcacac ccattgggga ggtaaatcgg aaaaaggagt tccgggtttt atccccgaat
     2041 cgggatcacc cagagaggaa tcgtaaatgt agccacaaac ctggcatcgg tagcgctctt
     2101 tttccttaat ctcttcgtga taagtggcag cggtacgagg aactcggcct ccttttatat
     2161 cccggtagta actataagtc attggttctc cacctttttc cataaaatcg gtctccacta
     2221 tttctcccaa aaatacccag tgagtaccag catctaactg attcactact ttagcttcta
     2281 agtaagctac tgtgtgttcg gtaataatgg gcattcccgt cttaccctgg aagtgcttaa
     2341 cctgggagag tttgtcagtg tttctaccac tgtggaaacc aaagagacca ataagaggta
     2401 agggggtgtt tttttccaga actgaaactg aaaaatagcc gcttttctgg atataggtag
     2461 aggttaaact ttctttgttt acactcacta ccagctgagg tgggaaagcg gtcacctgag
     2521 taagagcatt gacaataaga ccgttaaatt ttccttccca ccctgaacac aggatgtaca
     2581 tcccacaatt taaggtaaaa agagcatcta aattcaagat tagctctcct ttctatatat
     2641 cggctaactt tttcatagtt tctttagtat tctcatagag ctcccgataa atggagtaat
     2701 atttatcata aatgtgaagg ttgtcctgat ttactttaat tattgggcga aagtccaccc
     2761 attctttgat tcgttctggt ttatcaatta ctcccgtccc taaaccggcc aggaaagcat
     2821 caccgaaagg agcctctaca tctttggaaa gccttttcat atcaaatcca gtgatatcag
     2881 caaagatttg cacccagtaa gatgagcgag ctacaccccc tactatccag cactctggat
     2941 taagcttcaa gcctgctttt attccctctt ccatattgtg gcgcaaagag taagctgctg
     3001 cttccagcat tgcccgatag acatgggctc gattgtgata aagagacaat ccaaaaatgg
     3061 ttcctcgagc atcaggatcc cagataggtg aacgctcacc catgaaatag ggtaaaacta
     3121 ccactccctc gcttccggga ggaatacctt gagtctgtaa ttctaataga gtataggctg
     3181 atacgccggt gtttttttct acttcttttt catattttcc aaattggtct ctgaaccacc
     3241 gagccagccc tccggatgta gcacttcctc caaaggtgta gatcagcttg tcatcgtaga
     3301 ctacgtaagg atagctaacc aataagggag tcagatattg tccttcatgc accgttcccc
     3361 agcacatcga agttccagcc atggctacgt gttctccctc agccagcacc ccggcagaaa
     3421 actgagctac tggagcatca attcctcctg atacaaccgg agtcccttcc tgtaagccag
     3481 tttctttcgc aaaccgggcg ttaagctttc ctactatatc ggaggacttt aaaattttct
     3541 cagggagcat tcttttggga atacccagaa tttcacacat ttcctctgac caggtacgct
     3601 ttcggatatc aaaaactccc cccagatttc ctgctgagga ataatcagta gctaagacac
     3661 cggtgaggtg gtaaattaca tagtctttgg gggtaacaaa ctggtagatt tttttccaca
     3721 cttctggttc atgattgcga atccacatca ttttggtaaa gccaaagtaa gaatcgacgt
     3781 agtttccagt aattccaaaa atcttttcct gggggacatg gttttttacc cactgagttt
     3841 cttctctggc tctgcggtcc atccagatta agcagggata aagaggcttt atttcttcat
     3901 ctactggaac gcctgagccc ccatagagac cgctgatagc taatccggca atctcagaag
     3961 gagctacttt agatttttct acacatcgag agatggtttt aatcactgcc tccagccata
     4021 cctcaggcca ttgttctgcc caggaaggtc ggggagtgat aacttcatac tcttgaaaag
     4081 catcggctat aaactcacct ttttcattaa ccatgactgt tttggtcccc tgtgtaccaa
     4141 tatctgttcc taaaagatac atttttttat tcaccccttc agctctaatt tctgctattt
     4201 taacccactt tgacgaatta ctcaaaacct gttttttaag gaaattgctt tataaagttt
     4261 ttagaaggtt tataatagca ttttgattct tcctgacttt gacagctata atttaaaaac
     4321 attgatatat caaggaataa agagtctctt ttttgtttac tccactacag tttcactgag
     4381 aataaacttg ggaatcttta tccctaaagc tctaaatatc tgaacattat cattctctat
     4441 ggcctttatt tcttttgttt tcgtaactac acagaggtgc atatttttat tgaatc
//
LOCUS       DAOSVE010000115         4455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_8386_length_4455_cov_76.708409, whole genome shotgun sequence.
ACCESSION   DAOSVE010000115 DAOSVE010000000
VERSION     DAOSVE010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8386_length_4455_cov_76.708409"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="PK016_07920"
     CDS             <1..948
                     /locus_tag="PK016_07920"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXL03020.1"
                     /translation="AINTKQMLQLFFEAIKSPAFREEEIERERTFLLNILHSLDDDPF
                     QAAMLRFKKAFYGRHPYAFPTMGEENTLRNLGEEEIWAWYRKVYIPNNMVVAVVGDFD
                     PDEVEKIFYEEMGEIIPKEPLIPSIEKFSPFSLKIVDRKEVRDSWMVVGFRAPGLLEE
                     KERAVFDILNNILGGGMYSRLFLKIREERGLSYQVGSLYVPLRGPSFICAYASFPYAY
                     FDTVVGILKEELRGLSHLDKEEFEEAKNYTRGNFLHCLEMVFSLSSLFAFFERVGLGW
                     DFIFRYEKTLKEISLEEARAIYQRFSGQGESIGGIVPAE"
     gene            complement(1027..2466)
                     /locus_tag="PK016_07925"
     CDS             complement(1027..2466)
                     /locus_tag="PK016_07925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL03021.1"
                     /translation="MREEYSSSPYTHRYQILAAVMLCGIMGPIDASVVNVNYHIIGSR
                     FGVALTSAQWVVMVYLLTISSLLPFYGRLGDIWGYKRVYLIGLAGFTITSLLCSVSYF
                     LSSISWLILFRAIQGITAGMMMSVPYAIIVASFPPEERGRALGINAISVSTGLAIGPS
                     LGGFITHFAGWPYIFLINIPIGIVGLWWGNKIIPELKGKPGRIDGRGAITNCLFLFSF
                     LLFLNRFPTQGIHSTTLLTAAVAVVAFFSFLTIEKRANPPLINLELFHNLSFSLANLC
                     ALLNFMSQYILVTLTPSYLNRVLRYEQNEVGLILTIFPLTTLAVAPFAGSLSDRTSPH
                     FLTFIGAALCALSLFSLSQLPASISTAGVAWRLALFGLGTGIFQSPNMNVAMGSVPRE
                     HLGVASSILATVRNVGMVLGTAMGGGILNASVPTSILQGLGKLIGEEASIFMLGLKNA
                     YFWGAILTGIAALVSLFIPHYNLVNKECS"
     gene            complement(2502..3911)
                     /locus_tag="PK016_07930"
     CDS             complement(2502..3911)
                     /locus_tag="PK016_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HXL03022.1"
                     /translation="MEEKTIEDEEVVLEGEAFSTILPEILDKKHIRSKAFSLSLPALL
                     NMLLISFVGMVDMIMVGRLGPAAISAVGMVNQPIFLIVSVFIALTVGTTALVARFIGA
                     KDINSAKNVAKQSMVVSILFGILLLPILYIFAPQIEKAMGAEPEVLTLGVAYMRTIAI
                     GTPFNVLAMGLGAILRGSGDMKTPLIADIIANLSNILGNYILIFGKFGAPALGVAGAG
                     IATSIARFISSIILLYVLYRGKTVIKLSLKENYGLDFGILKRIFNIGIPSALEQFVLR
                     AGQLTFVRIVAELGTIAFATHQIAMNIQSLSFMPGQAFSMAATTLVGQLLGANKPDIA
                     EESARQTRLLGMIVSGISAFTIFFFGKYITMLYTNDIAIIEQSRICLRIIALIQPAQS
                     TQFILAGALRGAGDTRFPLYSTIIGIWGMRVALAYLFVMTFGWGLTGAWLAIGFDQIL
                     RAIVIYSRFRSGKWKHFEV"
BASE COUNT         1368 a          827 c          931 g         1329 t
ORIGIN      
        1 gctatcaata cgaaacaaat gttacaatta ttctttgaag cgattaaaag cccggctttt
       61 cgagaggaag agattgaaag agaaagaact tttcttttga atatccttca ttctctggat
      121 gatgaccctt tccaggctgc gatgctgcgg tttaaaaaag ctttttatgg tcgtcatcct
      181 tatgcttttc ccaccatggg tgaagaaaat actctccgca atctgggaga agaagaaatt
      241 tgggcctggt ataggaaagt ttacataccc aataatatgg tagttgcggt ggtgggtgat
      301 tttgaccctg atgaagtaga gaagattttt tacgaagaga tgggagaaat tatacctaag
      361 gagcccctta ttccttctat agaaaagttt tctcccttct cattaaaaat tgtggaccgt
      421 aaagaagtga gggatagctg gatggtggtc ggttttaggg ctcctgggtt attagaagag
      481 aaagagagag cagtttttga tattctgaat aatattttgg gagggggtat gtattctcgc
      541 cttttcctga aaatcaggga ggaacgaggc ttgagctatc aagtaggttc tctttatgtt
      601 cctctgcggg gtccatcttt tatctgtgct tatgccagtt ttccttacgc ttattttgat
      661 acggtggtgg gaattttgaa agaagaactt cgaggattat ctcatctcga taaagaagag
      721 tttgaagaag ccaaaaatta tacccgaggt aattttcttc attgtttaga aatggttttt
      781 tccctttcct ctctttttgc tttttttgag agggtagggt taggttggga ttttatcttc
      841 cgttacgaaa aaacgttgaa agagatttct ctcgaagaag caagggctat ttaccaacga
      901 tttagtgggc agggagagtc tataggaggt attgtccctg ctgaatagct gtagtgggtt
      961 ttgcgggatg ccataattta aagaaatacg gttctgtgca ggttttatct gagcgaaaca
     1021 ttaaagttaa gagcattcct tatttactaa gttgtaatga gggataaaaa gagaaacaag
     1081 agctgctatc ccggttaaaa ttgctcccca gaaataagca ttttttaatc ccagcataaa
     1141 aatggaagct tcttcaccta tcagttttcc taatccttga agaatagaag tgggaactga
     1201 ggcattaaga atcccgcctc ccatggcagt tcccagaacc attcctacat tacgcactgt
     1261 agctagaatg cttgaagcca cacctaagtg ttctcgaggt acacttccca tagcaacgtt
     1321 catattggga gattggaaaa tcccggtgcc taaaccaaag agagctaatc tccaggcgac
     1381 gcctgctgta ctgatagagg caggtaactg gcttaaggaa aacaagctta aggcacaaag
     1441 agcagcgcct ataaaagtaa gaaagtgagg actagtacgg tcagagagtg aaccagcaaa
     1501 gggggcaact gccaaagtag tgagagggaa aatagttaag ataagtccta cctcattttg
     1561 ctcatatcgt aatactcgat taagataaga gggggtaaga gttaccaaaa tatactggga
     1621 cataaaattt aagagagcac ataggttagc taaggaaaag gacaagttat gaaataattc
     1681 taaatttatt agtggagggt tagctctttt ttctatagtc aaaaaactga agaatgccac
     1741 aactgcaact gcagcagtaa gaagagtggt agaatgtatc ccctgggtag gaaaacgatt
     1801 tagaaacaag agaaaagaaa aaagaaacag gcagttggtt attgctcctc ttccatctat
     1861 tctacccggc tttcctttta attcgggaat aattttattt ccccaccaca ggccaactat
     1921 accgatgggg atatttatta agaagatgta aggccaacca gcaaagtgag tgataaaacc
     1981 acctaaggat ggcccgatag ctaatcctgt ggatacactg atagcattga ttcctaaagc
     2041 tcggcctcgc tcctcgggag ggaaagaggc aacgattatg gcataaggta cagacatcat
     2101 cattccggcg gtaatcccct gaatagcacg aaaaagaatt agccaggaaa tagaggatag
     2161 aaaataagaa acactacaca gaagagaagt aatagtaaaa ccagcaaggc caattaaata
     2221 tactctttta tatccccaga tgtcacccag acgaccataa aagggaagca aactgcttat
     2281 ggtcaaaaga taaaccataa ctacccattg agcgctggtg agagcaacgc caaagcgact
     2341 tccaatgatg tgataattaa cgttgaccac actggcgtca atgggtccca ttattccgca
     2401 taacataacc gcggctaaaa tttggtaacg atgggtgtaa ggagagctac tatattcttc
     2461 tctcatctat ttttgaacct tattggttaa gaatagatag tttatacctc aaaatgtttc
     2521 cattttccag acctaaatcg agaataaatc actatagctc ttaatatttg atcaaaacct
     2581 atggctaacc aagcacctgt gagtccccac ccaaatgtca ttacaaaaag ataggccagg
     2641 gctactctca ttccccagat acctataatg gtggaataga gaggaaaacg ggtgtctcct
     2701 gctcctctca gagctccagc taatataaat tgggttgatt gagcgggttg aataagagct
     2761 ataatcctca ggcatatacg actttgttca ataatagcga tatcattggt ataaagcatg
     2821 gttatatatt ttccgaagaa aaatatggta aatgcgctta taccagagac tatcattcct
     2881 aacagtcttg tttgacgagc gctttcttct gctatgtcgg gtttgttagc ccctagaagt
     2941 tgacctacta aagtagtggc agccatagag aaagcttgtc cgggcataaa tgacagagat
     3001 tgaatattca tagctatctg atgggtagca aaagctatag ttcccaattc tgccactatt
     3061 cttacaaatg ttaactgtcc tgctcttaaa acgaattgct ccaaggcaga aggtattcct
     3121 atgttaaata ttcttttcaa gatgccaaaa tctaagccat agttttcttt aagtgagagc
     3181 ttgattactg tttttcctct atagagtaca taaagtagaa taattgagga gataaatctt
     3241 gctatggagg tagctatacc tgctccagca actcctaaag ccggagctcc aaatttgcca
     3301 aagattaaaa tatagtttcc taatatattc gataaattgg cgatgatatc agctataaga
     3361 ggagttttca tatcgccgga gcctctcagt atggctccca atcccattgc tagaacatta
     3421 aaaggtgttc ctattgctat tgttctcata taggcaactc ccagagttaa cacctcaggc
     3481 tctgctccca tagctttttc tatttgggga gcaaatatat atagaatggg tagcaatagt
     3541 ataccgaaca atatacttac caccatgctc tgttttgcca cgttttttgc gctgtttatg
     3601 tcttttgctc ctataaagcg agccactaat gcggtagttc ccactgtgag ggctataaac
     3661 acagagacga taaggaaaat gggctgattt accattccta cggctgagat agcagcaggt
     3721 cctaatcttc ccaccataat catatctacc atacctacaa aactgatgag aagcatattt
     3781 aagagggcag gtaaagacaa ggaaaaagcc ttggaacgga tatgtttctt gtccagaatt
     3841 tctggcagga ttgtgctaaa agcttctcct tctaaaacaa cttcttcatc ttctattgtt
     3901 ttttcttcca atttcatctt cctctaagat attatatagc ttttgtaaag cgtgtactgc
     3961 tttggcattt accttttgaa ggattaagca gtttcttttt atgtaaaggt aaaaaatagt
     4021 tttcttatca caccggattt cccctttccc tattttcttt tagctatttt ggaggaaaaa
     4081 ggattaagaa aaatgtgata aaaactttgt gagctattta gctgtggtac caaaattaaa
     4141 atactataat atccctcagt acatagcgac cttgagtttt tgactttcga tataatttac
     4201 tttttaaagt ttttaacatt ttaatcagtt ttgtgggata ctatattttg cttttaatct
     4261 tatgccgtag gctgcccgct tctcgtggtg atgggaggag gtgccgcagg ctgttcggtc
     4321 tcgcgatgac ggagaattac agtgagattt ttggttaact atgatatctc tcttgaactt
     4381 atatgattgg gtttacaggg tatagtttaa tctcccaggt agttattcct ttctatccat
     4441 ttatctgttt tgaaa
//
LOCUS       DAOSVE010000116         4197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_8937_length_4197_cov_75.128199, whole genome shotgun sequence.
ACCESSION   DAOSVE010000116 DAOSVE010000000
VERSION     DAOSVE010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8937_length_4197_cov_75.128199"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="PK016_07935"
     CDS             complement(<1..776)
                     /locus_tag="PK016_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4896 domain-containing protein"
                     /protein_id="HXL03023.1"
                     /translation="MWFLLVIFNALFIGVGIYLIHNSYYELGAILFIASFLIDLFILN
                     PRAYPYRYTIPALIFLLLLVVYPICFTIKTAFTNYGTGHIFTRQEAVERLLYDPNYTY
                     VESEEEIDFKVFSVFEDSKPTDEFILLFDISGELYIGGMPSPSKQRGKEILLREGKTY
                     KVTSEEVNADGKIFKLSPFPESLENIKVITSADKEYEPLYAPDDPALQKNVYHFIMLV
                     QKYLVNTEYLNQGQGKIIGIKVAADGKWKFFFVERLYRLA"
     gene            complement(867..2063)
                     /locus_tag="PK016_07940"
     CDS             complement(867..2063)
                     /locus_tag="PK016_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter substrate-binding
                     protein"
                     /protein_id="HXL03024.1"
                     /translation="MKGLKFLPLVLVFILVFSSISVAQTPEITIWCSEKQVDILQKLG
                     EEFKAQYGVSVNVQYVEFGSIKPNFLTAAPQGKGADIIVGAHDWVGELVVNGLLEPLP
                     PFEGQESFYSTALNAFSYGGKLYGLPYSMEAIALIYNKEYVEEPPKNMEELINIAERI
                     DATYKGEVRGFITSAAELYYAAPFILGFGGYVFKETPEGLDVTDIGLANPGAIKGAEF
                     WKKLVDEGILTTGDNYEIMDSLFKEGKAAMIINGPWAVKAYKDAGIDYGVAPIPDLEE
                     GLPAKPFVGVQGFMINAKSPNKVLARDFLVNFIATQDTMYKIYLADPRLPSREDVLSL
                     VEDDPDIVGFTQSAANGTPMPNVMEMAAVWGAMNDALNLIINGKASPEDALLNAVETI
                     KAQIGK"
     gene            complement(2404..3297)
                     /locus_tag="PK016_07945"
     CDS             complement(2404..3297)
                     /locus_tag="PK016_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXL03025.1"
                     /translation="MINKNTTNFSDAAIAKKYNTGYKKTFGRVRLLPLYFLLYLLLFL
                     YLVPFFLILVNAFKDRSQIVANPLGLPPAFKLINFIEAFQKMNYPYAFWNSLVITVLS
                     SILIVFFSSMAAYLFARAKWSFNKFMFFVMVASMLIPFQAIMIPLVKIYGALQLLNNK
                     WTLIYMYIGFGSSLAVFIYHGSVKSIPLELEEAAMIDGCSKLQTFFKIVFPLLKPTTT
                     TIIILDVLWIWNDFLLPSLILISPKNRTLPLSTYYFHGTYTSDYGLLLASLILAITPV
                     IIFYVFMQKHIIKGVLQGSIK"
     gene            complement(3294..4178)
                     /locus_tag="PK016_07950"
     CDS             complement(3294..4178)
                     /locus_tag="PK016_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXL03026.1"
                     /translation="MITEKTFLGKLKTYLLFAGPTTFIFFTVVILPFLFGIYLTLTNW
                     DGISATYSFVGLKNYLSAFQDKKFWDSFILTLKYVSYTVIFTNVIAFTLAYLLTSGIK
                     GQNFLRAGFFIPNLIGGIVLGLVWRFVFSNVLVYIGNSLNWEIFSRSWLSHPEKAFWT
                     LVIVSVWQSSGYMMIIYIAGLTGIPKDLIEAATIEGANSLQRIRHVILPLLAPFATVC
                     VFLSLQRGFMVYDLNIALTEGGPFKSTELVAMHVYEKAFLSQEYGIGQAEAFFLFAMI
                     VTVTLVQVYFSKKLEVEQ"
BASE COUNT         1384 a          829 c          780 g         1204 t
ORIGIN      
        1 tatgccagcc ggtaaagcct ctccacaaag aaaaacttcc acttaccgtc tgcagctact
       61 ttaatcccga tgatttttcc ctggccttga ttcaaatact cggtatttac caaatacttt
      121 tgaactaaca tgataaaatg gtaaacattc ttttgtagag cagggtcatc cggagcataa
      181 agaggctcat actccttatc tgcagatgta atcaccttta tattttctaa agattcaggg
      241 aaaggagaaa gcttgaaaat ttttccatct gcatttacct cttcagaagt gactttataa
      301 gttttacctt ctcttaagag aatttcttta cctctctgtt tggaaggaga aggcatgcca
      361 ccaatataaa gctctccgct tatatcaaat agtaagatga actcatcagt gggttttgaa
      421 tcttcaaaaa cggagaaaac tttaaaatct atttcttctt cgctttctac ataagtataa
      481 ttcgggtcat acagaagtct ctctacggct tcctgcctgg taaagatatg tcctgtacca
      541 tagttagtga aagcagtttt aatggtaaaa cagatgggat ataccaccag caaaagaaga
      601 aaaatgagag caggaattgt gtacctataa ggataggcgc ggggatttaa aataaaaaga
      661 tctatgagga aagaagcaat aaaaagtata gctcccagct cgtaatagga gttatggatg
      721 agatatattc ccacccctat gaatagagca ttaaatatta ctagtagaaa ccacaaaaga
      781 accttggcaa ttttaatttt aaatcttctc ccttttaata aatttgtgag cctgaagaaa
      841 tttcttcagg ctcacaaaag gaagacttac tttcctattt gagcttttat ggtttctaca
      901 gcattaagaa gagcatcttc tggtgaagct tttccgttga taataaggtt gagagcatca
      961 ttcatagctc cccataccgc tgccatttcc ataacattgg gcataggagt tccattagct
     1021 gcgctttgag taaagcccac aatatctggg tcatcttcta caagagacag cacatcttct
     1081 cttgagggaa gccgggggtc tgccagatat atcttataca tagtatcttg ggtagctata
     1141 aaattaacaa ggaagtctcg agctaaaact ttgttgggag atttagcgtt aatcatgaaa
     1201 ccttgaacac ctacaaaagg cttggctgga agcccttctt caaggtcagg gatgggtgct
     1261 acaccatagt ctatacctgc gtctttgtaa gctttcaccg cccatggtcc gtttataatc
     1321 attgctgctt ttccttcttt gaagagagag tccataatct cgtagttatc tccagtagtc
     1381 agaatccctt catctactaa ttttttccag aactctgctc cttttattgc tccggggttg
     1441 gcaagaccga tatcagtcac atcgaggcct tcgggggttt ccttaaatac gtatcctcca
     1501 aaaccaagta taaatggagc tgcatagtaa agttccgccg cactggttat aaaccctctt
     1561 acctctcctt tataggtggc atctattctt tcagctatat tgattaattc ttccatgttc
     1621 ttcggaggtt cttcaacata ttctttgtta taaattaagg ctatggcttc catgctgtag
     1681 ggcagtccgt aaagtttccc cccgtagctg aaagcattca gagctgtaga ataaaagctc
     1741 tcttgacctt caaaaggggg aagaggttcc agaagcccgt tgaccaccag ttctcctacc
     1801 caatcatgag ctcccacaat gatatctgct ccttttcctt gaggagcagc agtcaggaaa
     1861 ttgggcttta tagaaccaaa ctctacatac tggacattta cactaacacc gtattgtgcc
     1921 ttaaactctt cgcctaattt ctggagaata tctacttgtt tttcagaaca ccatatggta
     1981 atttcgggag tttgagctac agaaatagag gaaaacacca aaatgaacac caaaaccaaa
     2041 ggtaaaaatt tgagtccctt cattaataac cctccttttt gatttttggt ttaatttaca
     2101 gaacttagcg ttccagaagc ttttttttga cctcctttgc cggcttttca aaaccatccg
     2161 gactgtcttt attatatatt cccctctcat ctttttcaag ttaccgagtt gcctcgatga
     2221 aactaaagtg aaagtaaaat tattccccct aaaggagacc aaaaaatcat ttacaagtta
     2281 agccaaggat ataaggtggc tctatagtaa attgtggaag aagatatcta aatggttatg
     2341 aaatcgcctt acaacaaaaa atattcagtt atgcaaatag gtgaaactga catacagttt
     2401 cggctatttg atcgagcctt gtagtactcc tttgataatg tgcttctgca taaatacata
     2461 aaaaatgatt accggcgtaa tggctaagat taaagatgcc agcaatagcc catagtccga
     2521 ggtgtaggtg ccgtgaaaat agtaggtaga taagggtaaa gttctgtttt tgggagagat
     2581 caaaatcagg gaaggcaaaa gaaaatcgtt ccagatccac agcacatcca aaatgatgat
     2641 tgtggtggta gtgggcttta ataatggaaa aacaatcttg aaaaatgtct gcaacttcga
     2701 acatccatcg atcatcgctg cttcttctaa ttccagaggg atacttttaa cagaaccgtg
     2761 ataaataaaa actgcgagag aagagccaaa gccaatgtac atgtagatta aagtccactt
     2821 gttgtttaga agttgcaggg ccccatatat cttgaccaga ggaatcatga tggcctgaaa
     2881 tggtataagc atagaagcga ccataacaaa gaacatgaac ttattaaagc tccacttggc
     2941 ccttgcaaaa aggtaagcag ccattgaaga aaaaaagaca atcaagatgc tgcttaaaac
     3001 ggtaataaca agtgaattcc aaaaagcata aggataattc attttttgaa aagcttctat
     3061 aaaattgatc aatttaaaag ctggtggtaa acctaagggg ttagccacta tctggctcct
     3121 atctttaaag gcattgacca agattaaaaa gaacggaact aagtaaagaa ataaaagcaa
     3181 atataagaga aaatataagg gaagaagcct aacgcgcccg aaagtcttct tgtagcccgt
     3241 attatatttt tttgctatcg cagcatcact aaaattggtt gtattcttgt ttatcattgt
     3301 tcaacctcca acttcttgct aaaataaacc tgaacaagag taacggtaac aatcatcgcg
     3361 aacaagaaaa aagcctctgc ttgtccaatg ccatattcct gcgacaggaa agccttctca
     3421 taaacatgca tggccaccaa ttctgtactt ttgaaagggc cgccttcagt taaggctatg
     3481 tttaaatcat aaaccatgaa tcctctttgg agggacaaaa aaacacaaac ggtagcgaag
     3541 ggagccaaca aagggagaat tacatgcctg attctttgca aactattagc tccttcgata
     3601 gtagcggctt ctattaaatc tttagggatt cccgttaatc cagcaatata gataatcatc
     3661 atatatccgg aagactgcca aacacttaca atcactaatg tccagaaagc cttttctgga
     3721 tgagataacc aggaacgaga aaaaatttcc cagttgagac tattaccgat atatactagg
     3781 acattagaaa agacaaaacg ccagactaga ccgagaacga ttccaccaat aaggttggga
     3841 ataaagaagc cagctcttag aaagttttgc cctttaattc cactggtcaa taaataagct
     3901 agtgtaaaag caattacgtt ggtaaatatc acagtatagg aaacatattt taacgttaag
     3961 ataaacgagt cccaaaactt tttgtcctga aaagctgata ggtaattttt taaccccacg
     4021 aaagagtaag tagctgatat tccgtcccaa tttgtcaaag ttaaataaat accaaataga
     4081 aaaggaagga taacgacagt aaaaaaaatg aaagttgttg gtccagcaaa tagcaaatag
     4141 gtcttgagtt taccaagaaa agttttttct gtaatcattc catgcctctt gtgttaa
//
LOCUS       DAOSVE010000117         4014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_9375_length_4014_cov_73.133620, whole genome shotgun sequence.
ACCESSION   DAOSVE010000117 DAOSVE010000000
VERSION     DAOSVE010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9375_length_4014_cov_73.133620"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..556)
                     /gene="rpiB"
                     /locus_tag="PK016_07955"
     CDS             complement(98..556)
                     /gene="rpiB"
                     /locus_tag="PK016_07955"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HXL03027.1"
                     /translation="MYLVIASDHFGYPLKKVIVEHLKEKKIEFEDRGVSSEDEVVDYP
                     DVAREVGEEISKGKYQYGILICGTGIGMAMAANKIPGVYAACAHDIYSAERAKKSNNS
                     NVLTLGRHIVGPELAKMLVDAWLESEFQGGRSLPKVAKIKEIENKYLKGE"
     gene            complement(581..1351)
                     /locus_tag="PK016_07960"
     CDS             complement(581..1351)
                     /locus_tag="PK016_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-threitol dehydrogenase"
                     /protein_id="HXL03028.1"
                     /translation="MEGVNFNIDFSLPGKVALVTGGAQGIGKAIALLFAQKGADLVLV
                     DMAEEVKEVAQEIEGYGRKALPLIFDLTDFDKIPQIVDQSVKALGRIDILVNNAGVGL
                     LDDAENLSPEMWDKTMAINLKAPFMLSQAVGREMIKQGGGKIINISSQAGLIALDKHV
                     AYCASKAGLIGMTKVLALEWAEFGIRVNAVAPTVVLTELGKRAWAGEVGEAMKQKIPT
                     RRFAYPEEIAAGVLYLASDASDIVTGITLVIDGGYTIQ"
     gene            complement(1526..2392)
                     /locus_tag="PK016_07965"
     CDS             complement(1526..2392)
                     /locus_tag="PK016_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HXL03029.1"
                     /translation="MFKFGVDTFIWSEAFSEKDLWVIPKAKELGFEVLDISISHPESF
                     PTDLVKEKVEEVGIEVVTTTTLGEKTNLISPDPDIRAQGVKHMKLLVDINVELGSKIT
                     GGVNYAAWGYLPGRPRTEEEWNWSITAMREVAEYAREKGDLIIAAEPVNRFETHFLNI
                     AEDAVRYCKEIGTGNVRVHLDSFHMIREETSFRGAVETCGKEYLGYVHVCENNRGIPG
                     TGLVPWREFFQALKDIGYQGPLVIESFDPSFKELNRQCAIWRKFADTGEELAVKGLQN
                     LKAIAQEIERGY"
     gene            2607..3788
                     /locus_tag="PK016_07970"
     CDS             2607..3788
                     /locus_tag="PK016_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL03030.1"
                     /translation="MSQEASQRRAKALRFIVFMGLVSLFGDIVYEGARGVIGPYLALL
                     GASASVVGLVGGLGEFIGYALRLFSGFIADRTRAYWLFTFIGYGLLFSIPLLSLANTW
                     WVAAIFIILERAGKAFRSPARDTILSYATKEVGRGFGFGIHEALDQIGAIIGPLIFSF
                     ILFFGGDYRSGFSILWIPALIVIGILVIARNQLPHPEKLETSKELEEKDDKIFSRVFI
                     LYSLFTFLAVAGFANFPLISYHLKIKSIMSDVQIPTFYALAMGVDALIALLIGRAYDR
                     VGLRTLMVIPLFSLLIPFLAFSNSFASVIIGIILWGIVLGVHETIMRAAVADLTSISH
                     RGSAYGIFNTIYGLSWLLGGALMGILYEISVSYLIILAVVFELASIPILLVLQREQKA
                     R"
BASE COUNT         1091 a          948 c          682 g         1293 t
ORIGIN      
        1 gtttctaatc tctttacttt attctggtta gctattacaa aaccttctaa catctcctca
       61 attaagtttt ctggttggtt aatgaatttt ttcactttca ttccccctta agatatttat
      121 tttctatctc cttgatttta gctaccttag gaagggacct tcctccttga aactcacttt
      181 ctaaccaggc atctactaac attttagcta actctggccc tacaatgtgt cttcctaaag
      241 ttaagacatt ggaattgttg ctctttttag ccctttctgc cgaataaata tcatgagcac
      301 aggcagcata aactcccgga attttattag ccgccatagc catgcctata cctgttccac
      361 agattaaaat tccatactga tatttccctt tgcttatttc ctcccccacc tctcgggcta
      421 catcaggata atctaccact tcatcttcag agctaactcc tcgatcttca aattcaattt
      481 tcttttcttt aagatgttct acaataactt tttttaaagg atacccaaaa tggtcagaag
      541 caataactag atacataaaa aacacctcct ctttaattct ctattgaata gtataccctc
      601 catcgataac taaagtaata ccagtcacta tatcagaagc atcactagcg agatacagga
      661 ctccagcagc aatctcctct ggataggcaa aacgtcgagt aggtatcttc tgtttcatag
      721 cttcacctac ttctcccgcc caggctcttt tgcctaactc agttagaact acggtgggag
      781 caacagcatt aactctgatt ccaaactccg cccactccag agccaaaact ttagtcatac
      841 caataagtcc agctttactg gcacaataag ctacatgttt atccagagca atgagtccag
      901 cttgagagga gatattgatg attttccctc ctccttgctt aatcatctct cgtcctacag
      961 cttgagagag cataaaggga gcttttaagt taatagccat agtcttatcc cacatctccg
     1021 gggataagtt ctcagcatca tccagtaatc ctactccagc attgttaact aaaatatcaa
     1081 ttctccccag agctttaaca ctctggtcta ctatttgagg aattttatca aagtcagtga
     1141 ggtcaaaaat aagaggtagg gcttttcttc catatccttc aatttcctgg gctacttctt
     1201 taacttcttc tgccatatct accagaacta aatctgctcc tttttgagca aaaagtaaag
     1261 ctatagcttt accaatccct tgagctcctc cggtaactaa agctactttc cctggtagag
     1321 aaaaatcgat attgaagttt actccttcca taactattcc ttcctttcca agatttttat
     1381 ttgtccctct atcatcttat tgtccgtcat tcctgaatgt atctatcagg aatccagaca
     1441 atgagggggt aaaccccttt ctttttcatc accgcaataa ccttcttttt tcgtcaccct
     1501 tcatagtctg ccgtagactg cttttttaat atcccctctc aatttcttgg gcaatagcct
     1561 tgaggttctg caatcctttt actgctaact cctcaccagt atcggcaaac tttctccaga
     1621 tagcacactg tcgatttaac tccttaaaac tggggtcaaa ggattcaatg accaatggcc
     1681 cctggtatcc aatatcttta agagcctgaa aaaactctct ccagggtact aatcccgtcc
     1741 ctggtattcc tcgattattt tcacacacat gaacataccc taaatactct ttcccacagg
     1801 tctctaccgc tcctctgaaa ctggtctctt ctcggatcat gtggaaagaa tctaaatgca
     1861 cccttacatt ccctgtccct atttccttac aataacgtac tgcatcttca gcaatattta
     1921 aaaaatgagt ttcaaaccga ttaactggtt ctgcagcaat aatgaggtca cctttttctc
     1981 gggcatattc tgccacttct ctcatagcag taatagacca attccattct tcttccgttc
     2041 ggggtcttcc tggtagatac ccccaggcag catagtttac tcctccagtt attttagaac
     2101 ccaattctac attgatatct actaaaagtt tcatatgttt aactccttgg gctctgatat
     2161 ccgggtcagg agaaataagg ttggttttct cccccagggt agtagtagtt accacttcta
     2221 tccccacttc ttctactttc tcttttacta aatctgtggg gaagctctcc gggtgactaa
     2281 tggaaatatc caagacttca aaccctaatt ctttggcttt aggaataacc cacaagtctt
     2341 tctcggaaaa agcttcggac cagataaagg tatctactcc aaacttaaac attaagctac
     2401 ctccctttgc attcttatag gcttactctc aatcttattt ttatcatcac cgctattcta
     2461 ccacctttag atgtttaaaa ataatccttc gaaggaattg cgataatcgc taaaaacttc
     2521 tgtaatataa agcaaccttg aaaagacacg agagctggat ttataatacg agcattggta
     2581 acattaacaa taggaggaga aaaaagatga gccaggaagc ttcccaaaga agagccaaag
     2641 cactccgatt tatagttttt atgggtttgg taagtctatt cggtgatata gtttatgaag
     2701 gagcaagagg agttattggc ccctatttgg ctcttttagg ggcaagtgca tcagttgtgg
     2761 gcttagtcgg ggggttgggt gaattcatcg gatatgctct tcggcttttt tccggtttta
     2821 tagctgaccg aaccagagcc tactggctct tcaccttcat cgggtatgga ttactattct
     2881 ctatccctct cctttctttg gctaatacgt ggtgggtagc ggctatattt ataattctag
     2941 agagagcggg caaggctttc cgctctccag cccgggatac cattctctcc tatgctacca
     3001 aagaagtggg acgaggattc ggcttcggta tccatgaggc gttagaccag attggagcta
     3061 ttattgggcc tctgattttc tcttttattc tattctttgg cggtgattat cgttccgggt
     3121 tttccatcct ctggatacca gctttaatcg tgataggcat tctggtcatt gccagaaatc
     3181 agctacctca ccctgagaaa ttggaaactt ctaaagaatt agaagagaaa gacgataaaa
     3241 tattttcccg ggtattcatt ctctattctc tatttacttt tcttgctgta gcgggatttg
     3301 ctaactttcc gctcatttct taccatctca aaataaagtc aatcatgagc gatgtccaaa
     3361 tccccacctt ttatgcctta gctatgggag ttgatgcttt aatagctctt ttaataggaa
     3421 gagcctatga tcgagtagga ctgagaactc taatggtcat acctttgttt tctcttctta
     3481 tccccttttt agctttttcc aatagttttg cctctgtgat tatcggaata attctatggg
     3541 gaatagtttt aggtgttcat gaaaccataa tgcgggcggc ggtagctgat ctaacctcta
     3601 taagccaccg aggctcagcc tatgggattt tcaataccat ttatggacta tcctggttac
     3661 tgggaggagc tttgatggga atactttatg aaatctctgt ttcttattta attattctgg
     3721 ccgtggtatt cgagttggcc tccatcccca ttttactggt tttacaaagg gaacaaaagg
     3781 cccgataaac attaaatctc gctaaatgtt aaaacccggg attgccaggt aaagagtaag
     3841 cgaggcttac aatgactata aaggggcaaa accctcctat aacccttttt tgttgtcatt
     3901 tctgaatgtc tctatcaact acgaggggaa aaagaccccc tcataacccc cagaaacaaa
     3961 agactaaaag acaaaaaagc aaaagctact gaggaagaaa aaaccttcct cagt
//
LOCUS       DAOSVE010000118         3899 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_9659_length_3899_cov_73.582986, whole genome shotgun sequence.
ACCESSION   DAOSVE010000118 DAOSVE010000000
VERSION     DAOSVE010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9659_length_3899_cov_73.582986"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..799)
                     /locus_tag="PK016_07975"
     CDS             complement(29..799)
                     /locus_tag="PK016_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL03031.1"
                     /translation="MSDSFWYVDQLTGEWINSLAEEEPIYNEDYTQMTAKLRKGIYWS
                     DGVEFTADDVAFTVEYLMKTPGMRWSAELNQYVESVSKPDDYTVIFKLKEPNNRFHYY
                     FVFRFDALYMMPKHYWENVTDPLVDNFYPPISLGAYVLKNADPSGYWTLYERREDWQR
                     TSAGVITGKPGPEYIMSVFYNTSTAKAMAMERNELDLLMDLDTETFIEVTNKNPNVRS
                     WYKDFPWAFPDEIDQRSIGFNLEKYPYNLRNKTIASLF"
     gene            complement(1451..2455)
                     /locus_tag="PK016_07980"
     CDS             complement(1451..2455)
                     /locus_tag="PK016_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HXL03032.1"
                     /translation="MKALVLEKPAPIETNPLLTYDLPIPQPQKEEVLIRIKACGVCHT
                     DLHIVEKEIPPHLLPLVPGHQIVGIIEEVGERVKDLLPGELVGVPWLNRVCGNCRFCQ
                     EGRENLCESALFTGYDVPGGYAEYVATSKEAVYRLPEGYKEEELAPLLCGGVIGYRAY
                     RMSEVKKGDTLGIFGFGSSAHVVAQIAKIEGVEVFVFTRSLEHQILARDLGASFVGTS
                     EDRPPNPLNAIIIFAPAGNLVKNALLYLERGGKVIMAGIYSTPLPETPYSFLYWERSI
                     KSVANSTRRDVRELLDFAQKFHLEVKSEVFPLTEANHVLQLLKKSKIKASAVLKPELL
                     "
     gene            complement(2452..3375)
                     /locus_tag="PK016_07985"
     CDS             complement(2452..3375)
                     /locus_tag="PK016_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXL03033.1"
                     /translation="MAKVLVSGGAGFIGSHIVEAYLASGFEVVVVDNLSTGKRENLPP
                     GVTFYEGDITSPLDMEEIFSRERPDYVNHHAAQINLRRSTEEPLFDAQVNILGSISLL
                     ELSVKYRVKKFIFASTGGAIYGEVETPPAHEDMPTLPLSPYGVAKRSVELYLNYYHKV
                     QGLDFVALRYGNVYGPRQDPRGEAGVIAIFISRIIKGEPCIIFGDGRKTRDYVYVKDI
                     AKANLLALSVSGVYNLGTGKETSVLELVEFLKKVSGRDFQVIFEEERKGEINRIALCS
                     EKARETLGWTPSFSLEEGISQTWKWFKETKQ"
BASE COUNT         1205 a          826 c          674 g         1194 t
ORIGIN      
        1 tggttgtaaa agggattctt tcaacaattt agaataagga tgcaatggtt ttatttctca
       61 ggttataagg atatttctcc aggttgaaac caatggatct ttgatcaatt tcatcaggaa
      121 atgcccaggg gaaatcctta taccaggatc tgacgtttgg gtttttgttg gtaacttcga
      181 taaaagtttc tgtgtctaag tccataagca aatctaattc gtttctctcc attgccatcg
      241 ctttagcagt agacgtattg taaaaaacac tcattatata ttcgggacct ggtttacctg
      301 taattactcc agcggaagtt ctttgccaat cctctcttct ctcatatagc gtccaatagc
      361 ctgagggatc agcattcttt aaaacataag ctcctaaaga aattggggga tagaaattat
      421 ctacaagggg atcagtcacg ttttcccagt aatgcttcgg catcatgtat agagcatcaa
      481 atcggaatac aaaataataa tggaatcggt tatttggctc ttttaattta aaaataaccg
      541 tataatcgtc tggtttactc acactctcta catattgatt tagctctgcg ctccatctca
      601 tccccggagt tttcattaaa tattcaacag tgaatgctac atcgtctgct gtaaactcaa
      661 ctccatcact ccagtatatt ccttttctga gcttcgcagt catttgggta tagtcttcat
      721 tatagatagg ttcttcttct gctaaagagt ttatccactc accagtaagt tgatcgacat
      781 accaaaaaga gtcagacata aaagtttgtc tgatgtgatt tggaacagta gccatccata
      841 aattataatt atttggatat gaatatcgag ttatttggtc tattaggaat atctgtagca
      901 atggtttgcc ctacaacaat agcctgagaa aaaaaacatc aaactacaaa aggtaattag
      961 taacaatacc attatctttt tcattcccaa tttctccttt tcgatttagt ttccaaaagc
     1021 tctctttaac taacctcaaa tttcacaccg ctaccaatat taaccgattg gttattgata
     1081 aacagaattc agaatctttt taatcacctc tccttaaagc tcttattaaa gtttctgcaa
     1141 cttttacatc agtatagatt cttatttata gttaaccgca aaatccatta taatgactat
     1201 gaggaggaaa aagacccccc tcataacccc ccaaaagcaa gaaacaaaga agcaaaatgc
     1261 tactggaaat attatatagt atccctcagt acatagcgac cttgagtttt tgactttcga
     1321 tataatttac tttttaaagt ttttaaccag ttttgcggga tactataaag gtataagaag
     1381 ttgcttagat atttcttatt cctctcccat tttctcgagc ttttctaaaa gaggccataa
     1441 agaagcctcc ctatagtagt tctggcttga gtactgctga agctttaatt ttactttttt
     1501 tcaataattg caaaacgtga ttggcctcag taaggggaaa aacctcgctc tttacctcca
     1561 ggtgaaactt ttgagcaaaa tctaaaagtt ctctgacatc tcgacgggta ctgttggcca
     1621 cacttttaat acttcgttcc cagtaaagga aggaataggg agtctctggt aagggtgtag
     1681 aataaatccc tgccattatc acttttcctc ctcgctccag ataaaggaga gcgttcttaa
     1741 ctaaattacc agcaggagca aatattatga tagcatttaa aggattagga gggcggtctt
     1801 ctgatgttcc tacaaaagaa gctcccagat ctcgagctaa aatctggtgt tcaagacttc
     1861 tggtaaaaac aaatacttct actccttcta tttttgctat ctgagctaca acatgagcag
     1921 aggagccaaa tccgaaaata cccagagtat ctcctttttt tacttcactc attcggtaag
     1981 cccggtaacc aatcactcct ccacataaaa gaggagccaa ttcctcttct ttatacccct
     2041 ccggtagtct atatactgct tcttttgatg tggctacgta ttcagcatac cctcccggaa
     2101 catcataacc agtgaaaaga gcactctcac ataaattttc tcttccttcc tgacagaagc
     2161 gacaattccc acacacccgg tttaaccagg gaactcccac caattccccc ggtaataggt
     2221 cttttaccct ttcccctact tcttctatta tccccactat ctgatggcca ggaacgagag
     2281 gcaaaaggtg aggtggaatt tctttctcta caatgtggag gtcagtgtga caaactccgc
     2341 aggctttaat ccggattaaa acttcttctt tctggggttg tggaatagga aggtcataag
     2401 ttaaaagagg gttggtttct ataggagcag gtttttccag tactagagct ttcattgttt
     2461 tgtttcctta aaccatttcc aggtctggct aattccttct tctaaagaaa aagaaggagt
     2521 ccaacccaga gtttctctgg ctttttccga gcaaagagca atacggttta tttctccttt
     2581 tctttcttcc tcaaatatca cctggaaatc tcttccgcta acttttttca aaaattctac
     2641 cagctctaaa acactggttt ctttccctgt tcctaaatta tatactcctg atacagaaag
     2701 agctaagaga ttggcctttg ctatatcctt tacataaaca tagtctctag tttttcgccc
     2761 atcaccaaaa atgatgcaag gttcaccttt aattatccgg gagataaaga tagcaataac
     2821 tcctgcttct cctcgagggt cctggcgtgg accataaaca ttaccgtagc gcagagccac
     2881 aaaatccagg ccttgtactt tatgatagta gttaaggtat agttctaccg aacgtttggc
     2941 cactccataa ggagaaagag ggagagtagg catgtcttcg tgagcaggag gtgtttccac
     3001 ttctccatag atagctcccc cggtggatgc aaaaatgaat tttttaaccc ggtatttaac
     3061 actcaactct aaaagagaaa tgcttcccag gatattcacc tgagcatcaa aaagaggctc
     3121 ctctgtagaa cggcgcaaat taatctgtgc agcatgatgg ttaacatagt caggtctttc
     3181 ccgggaaaat atttcctcca tatcaagtgg agaagttatg tctccttcat aaaaagtcac
     3241 ccctgggggt agattctctc ttttaccagt agagagatta tccactacta ctacctcaaa
     3301 gcccgaggca agataggctt ccactatatg agagccgata aaacctgctc ccccgctcac
     3361 aagtacttta gccacattat ttctcctttt gttcttatta ctaatataaa agcacaaaat
     3421 ctatctcaaa taaaccccct ctggagtaaa ttagtccagg agaaagctat tatagctccc
     3481 ctgaagagag actttttctg tacagagaga aaaattacaa aagcttttgt aacaccgcaa
     3541 tttatagtta accgcaaaat ctattataaa gactataaag gggtgctcct ctttatagtc
     3601 ccccaaaaaa gataaaggaa aaagataaga aacttaaaaa aaaacaaaaa ctactggtgg
     3661 gaaaatatcc accagtatgc tgagaaaaaa gcagtctgcg acgcaagatt aggaaaactt
     3721 aaaaaacaaa agaaataaag gccatagaga aagacaaagt ctttctctat gcgtagcgac
     3781 cttgagtttt tgactttcga tataatttac tttctaaagt ttttaaccag ttttgcggga
     3841 tactataata gacgctctct ataagtgaga attggggaaa gaagtaagtg ataatatag
//
LOCUS       DAOSVE010000119         3871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_9764_length_3871_cov_87.617138, whole genome shotgun sequence.
ACCESSION   DAOSVE010000119 DAOSVE010000000
VERSION     DAOSVE010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9764_length_3871_cov_87.617138"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..759)
                     /locus_tag="PK016_07990"
     CDS             complement(172..759)
                     /locus_tag="PK016_07990"
                     /inference="COORDINATES: protein motif:HMM:NF014048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HXL03034.1"
                     /translation="MKELKRGRNCQSQVYTETVSYLDLKNKEVFIPKFTWDKNSQNIP
                     EVFKFQELILQSLIRHHLSDANKLQKFFDGMGMNDLNGNDFEILGEKALPEGHIDLLI
                     KDAQPIGISRKIIIEVKTENANVIDVRQLRNYIDEIKSECKAGILIAKHFTKKVLGEA
                     KSNGIACCVYSFKNVNKSSEYSFEELLSKLELVFQ"
     gene            920..1657
                     /locus_tag="PK016_07995"
     CDS             920..1657
                     /locus_tag="PK016_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL03035.1"
                     /translation="MFIKRTRGGSKEKPIYYLQLVQSYRDESGKPKHRVICTLGREED
                     LINSGTIDSLAQKFADLSDKFILLDKEKDSLGYTYLLGPLLIWDTLWKRLGIEEILNQ
                     VKKNYHIQFNFPKAVKLMVFNRLMDPQSKLSITTWKEKLYGQEFQEVELQHLYRALDL
                     LAENKEKIEKRLGKRTLTLFKPEVKLVFYDLTTIYFESQIVDDLRRFGYSKENRTDCT
                     QVVMGLVLSDRNIPLCETRLFRTKRAE"
     gene            1783..3342
                     /gene="istA"
                     /locus_tag="PK016_08000"
     CDS             1783..3342
                     /gene="istA"
                     /locus_tag="PK016_08000"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HXL03036.1"
                     /translation="MSNYLTLVRAVYSGISQRQVAKSHQVSRNTIALVLQHAQKRGWL
                     TLASLASISEEEFCCDFHLAKPPARDLSYTWPDYNWVHQELSKPYVTLKLLWEEYVAQ
                     CVQSGKRYYMETQFRQHYHEYAQQFKSTIRLEHKPGLALEVDWAGTKIKYFDQEKVTW
                     SEASLFISVLPCSQLIYAEPFPDQSLSSWISGHVHGFQYFNGVPKTIIPDNLKTGVKQ
                     SNFYQPVITKAYQEMADYYQTIILPARVRKPKDKPSVENGVLISSRKILAKLRNKQIL
                     SFSNLQELISAALEEINQSPLTGKNESRWSSYLAEEKDYLLPLPARSYELAEWSQAKV
                     QPNCHIAFQHKFYSVPFEYLGKEVDIRSTSSTIEILYQHERIASHKRLWGKVTYSTVK
                     EHMPPAKTFFTDWNGERFLEWAKKCGPETQAVVQAILDSAAIEQQAYRSCFGLLALRE
                     EYGELRLERACQLTRQRSPQPTYRHVKSILKRNEDQFSKETGIQPPVLKKATGFRRGA
                     NYFAREEKDPC"
     gene            3387..>3871
                     /locus_tag="PK016_08005"
     CDS             3387..>3871
                     /locus_tag="PK016_08005"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXL03037.1"
                     /translation="MVEAYLDQERNNNYEAMSFEDRLGLLVDWEFTKRQHNRLDRLIK
                     AAKFTDSTACIENIDYSPDRHLDRNLILELASCNYIPHARNIVINGATGAGKSYLAQA
                     LGISACRHHLPTRYFQLPDLLDELHLAKANEQEQFIKFKHQLAKIPLLIIDEWLLFPI
                     T"
BASE COUNT         1196 a          803 c          751 g         1121 t
ORIGIN      
        1 gcaacggact tctaatccgt aggttggggg ttcaattccc tccaggcgcg ccaaggcttt
       61 acagagccat tttcgccttc cctaccctct ctaaaaaacc cctgagtccg acgactataa
      121 gaagatgctt aatgattgtt gtatttgtgt tcttcagcta caatactccc tctattgaaa
      181 aactaactcc aattttgata ggagttcttc aaaggaatat tcagagctct tattaacatt
      241 tttaaacgaa taaacacagc aagcaattcc attgcttttt gcttcaccta aaactttctt
      301 ggtaaaatgt ttggctatta aaatgccagc tttacattca cttttgattt catcgatata
      361 gtttctcaat tgcctcacat cgataacatt tgcattttcc gttttaacct cgataataat
      421 ttttcttgat atacctattg gttgtgcatc ttttatgagg agatctatat gcccttcagg
      481 taaggctttt tccccaagta tttcaaaatc atttccattt aaatcattca ttcccatacc
      541 atcgaaaaat ttttgcaatt tgtttgcgtc tgagagatgg tgacgaatta atgattgaag
      601 tattaactct tgaaatttaa atacttcagg tatattttgg gaatttttgt cccaagtaaa
      661 ttttggtata aaaacttctt tatttttaag atctaaatag ctaacagttt cagtatatac
      721 ctgactttga cagtttctcc ctctttttaa ctccttcaat ctctgaaacc cttgatacat
      781 ctatattcta tttttctatt tctcattttt tctttatttt gtagtggcat tattttgtga
      841 tgtttttatg tttttctatt gacattaggt attgtatata gctattatgt agtaaaagag
      901 agagaagagg ggtagaagaa tgttcatcaa aagaaccaga gggggaagta aagaaaaacc
      961 catttattat ctccagttag tacaatccta tagagatgaa tcaggtaaac ccaaacatag
     1021 agtaatatgc actttaggaa gagaagaaga cttaataaac tctggaacta tagattcctt
     1081 agctcagaaa tttgctgatc tatcagataa gtttatcctc ctggataaag agaaagattc
     1141 cttgggatat acctatctct taggtcctct tcttatctgg gataccctat ggaagagatt
     1201 gggtatagaa gagatattaa accaggtaaa aaagaactat cacatccaat ttaacttccc
     1261 taaagcagta aagctcatgg tgttcaatag attgatggat cctcagagta aactatctat
     1321 aaccacatgg aaagaaaagc tctatggtca ggaattccag gaagtagaat tacagcatct
     1381 ctatagagct ttagacctct tagctgagaa taaagaaaag atagagaaaa gactgggaaa
     1441 aagaactctc actctcttta agccggaggt aaagttagta ttttatgacc ttaccaccat
     1501 ctattttgaa agccagatag tagatgactt acggaggttt ggatacagta aagaaaacag
     1561 aactgattgc actcaagtag ttatgggatt ggttttaagt gatcgtaata tccctttatg
     1621 cgaaacacgg ctgtttagaa ccaagcgcgc ggagtaaaag cgccagtagc gcggataatt
     1681 tggagccagt agcgcgccca tttagtacca ccttaacaaa ccaaatcctg taggattatc
     1741 tcatgccacg aaagtggtaa atctttttac aggaggtttg atatgtcaaa ttacttaacc
     1801 ttagtgcgtg ctgtttacag tggtataagt caacgacagg tagctaaaag ccaccaggta
     1861 tctcgtaaca cgattgccct tgttctacag catgcccaaa agagaggatg gctaacgctt
     1921 gcatcattgg cgtccatcag tgaagaagaa ttttgctgtg attttcatct ggcaaagcca
     1981 cctgccaggg atctttctta cacctggcca gattacaatt gggttcatca ggaactatcc
     2041 aaaccctatg taactttgaa actgttatgg gaggaatatg tagctcagtg tgttcaaagt
     2101 gggaagcgat actacatgga gactcaattc cgtcaacact atcatgaata cgctcagcag
     2161 tttaaatcaa ctattcgttt agagcataaa ccgggtttgg cgctcgaagt cgactgggca
     2221 ggaaccaaaa ttaagtattt tgaccaagag aaagtaactt ggtcagaagc atcactcttc
     2281 atcagtgttt taccttgtag tcaacttatc tacgccgaac cattcccaga tcaatcttta
     2341 tcctcctgga tttcaggaca cgtccatggt ttccaatact ttaatggggt acccaagact
     2401 atcatccctg acaatctcaa aaccggggtg aagcagagca acttttacca gccggtcata
     2461 actaaagctt accaggagat ggcagactat taccagacta tcattctccc tgcccgggtt
     2521 cggaaaccta aagataagcc ttcggtagaa aacggggtac tcatttcttc cagaaaaatc
     2581 ctggcaaagc tgagaaacaa gcaaattcta tccttctcta acctgcaaga gcttatcagt
     2641 gctgccttgg aagaaattaa ccaaagcccc ttaaccggga aaaatgagag ccggtggtcg
     2701 agttatctgg cggaagaaaa agactacctg ctccccttac ctgctcgttc atatgaactt
     2761 gcagaatgga gtcaggcaaa agttcaacca aattgccaca tcgcctttca acacaagttc
     2821 tattcggtgc catttgaata cttaggaaaa gaggtggata ttcgctccac ctcatcaacg
     2881 atagaaatat tgtaccagca tgagcgcatt gcttcccata aaagactctg gggaaaggta
     2941 acctattcaa cggtcaaaga acacatgcct ccggccaaaa ccttttttac tgactggaat
     3001 ggggaacgtt ttctcgaatg ggcaaagaaa tgcggtcctg aaactcaagc tgtggttcag
     3061 gcaattctgg atagtgccgc tatcgaacag caggcctatc gttcttgctt tggcttgctt
     3121 gccctaagag aggaatacgg tgagcttcgt ctggaacggg cctgtcagct tacacggcaa
     3181 aggtcccctc agccaactta tcgtcatgta aaaagcatcc ttaaacgaaa tgaagaccag
     3241 ttttctaaag aaacagggat tcagccccca gtgttgaaaa aagctaccgg ttttcgcaga
     3301 ggagcaaact attttgccag ggaggaaaaa gacccatgtt aacccaagaa acattaaaca
     3361 aactccgaca gatgaaactc aacggcttgg ttgaagccta tctggatcag gagagaaaca
     3421 ataactatga agctatgtct tttgaagatc gtttagggct acttgtggat tgggaattta
     3481 ccaaaagaca gcataatcga ttagatcgcc tcatcaaggc ggcaaaattt actgacagca
     3541 cagcttgtat agaaaacatc gactattccc ctgatcgaca tttagatcgt aaccttatct
     3601 tggaattagc ttcttgcaat tacattcctc atgcaagaaa cattgtgatt aatggagcaa
     3661 cgggtgctgg aaagagctac ctggctcagg ctctgggtat ttcggcttgc agacaccatc
     3721 tcccaaccag atattttcaa ctacccgacc ttttagatga gcttcatctg gcaaaggcca
     3781 atgaacaaga gcagtttatt aagttcaaac accagctagc aaagattcct ttacttatta
     3841 tcgatgaatg gcttctcttt cctatcacag a
//
LOCUS       DAOSVE010000120         3765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_10097_length_3765_cov_69.863342, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000120 DAOSVE010000000
VERSION     DAOSVE010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10097_length_3765_cov_69.863342"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..532
                     /locus_tag="PK016_08010"
                     /pseudo
     CDS             1..532
                     /locus_tag="PK016_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611272.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(568..1233)
                     /gene="rpe"
                     /locus_tag="PK016_08015"
     CDS             complement(568..1233)
                     /gene="rpe"
                     /locus_tag="PK016_08015"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HXL03038.1"
                     /translation="MIKVSASLDCADYLHLGEEVQKLERAKIDMLHIDIMDGVFVPNF
                     ALGTNLLKKLRPATSLLFDVHLMVQNPESFISLFADLGADMITFHAEVDSRLHQLVDR
                     VKRENKKVGLALNPSTPPDFLSYLLPYLDLVLLMTVDPGFVGQKFIPEVVPKVQRVRE
                     MVEEKDLNVDIAVDGGIGEKTVPLLKSAGANVFVAGTTSIFSGQEDVEKAALEFKKFC
                     EEC"
     gene            complement(1230..2249)
                     /locus_tag="PK016_08020"
     CDS             complement(1230..2249)
                     /locus_tag="PK016_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HXL03039.1"
                     /translation="MKALVLEEKGILSLREIQTPAPREGEALIRVQAAGICGSDLPRV
                     FGGQAYFYPLIPGHEFSGEVVEVGKKEDEEWIGKRVTVYPLIPCRNCRYCEAGQYELC
                     DNYGYLGSRQNGGFAEFVVAPVENLVLLPSCVSWEEGALSEPGAVTLHALRRVHPEMG
                     DRVAVFGLGPIGLLCGIWAKLGGARMLTGYDVDEQKFPLARASGFDLTLSPAGEKEEV
                     DLAIEASGSRRALLDALGIVRKKGKVLLVGNQEGEVVLKPQEISIVLRHELTILGTWN
                     SDFSSLDSDWERLLELEERGKVSLSPLISHRISLEEAPSFMLEMQQKRFPYTKVVIEP
                     QGVKL"
     gene            complement(2246..3280)
                     /locus_tag="PK016_08025"
     CDS             complement(2246..3280)
                     /locus_tag="PK016_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HXL03040.1"
                     /translation="MKAALLEDIDRLVVKEVEDYQVREGEVLVKVKSCAICGSDIRIM
                     HSGNPRVKFPHIIGHEIAGVVIEVGKGVEKFEVGDRVAVGADVPCGKCFWCQNGMGTN
                     CAINYAIGYQFPGGFAQYILLNALSVEQGSVHKIPEKLSFDEASLAEPLACAINGLEM
                     ANLGVGDALCIIGAGPIGCMMIEMGRHMGATNIIVIQRSQRRLDLARKFNADHYFLSD
                     KNVEEKIKEVTNGEGADVVMITCASPDAQEQALLLARHRARINFFGGLPSGARNISIP
                     SNLIHYRECMILGSHGSLPRHHRKALEVMEKGMVSPGEYITHRFPLEEIQEAFRVAES
                     HEGLKVVVNP"
BASE COUNT         1192 a          917 c          667 g          989 t
ORIGIN      
        1 ggtatagtag atatgcatta gcttgggagt tagctaatac cctggatgca gatttttgcc
       61 ttcagacact ggataaggca ttacttattt cccagcccga gatattcaac agcgatccgg
      121 gctctcagtt tacgagtggc gagttactga gaagctggag aaagcaggaa ttaaaatcca
      181 aagagatgga agaggccggg tatttgataa tatcttcata gaaagactat ggagaacagt
      241 aaaatatgag gaggtgtata tccacagcta tgatacagtg aggaaagcca gagagagttt
      301 agataattat ttccgattct ataacggtga gaggcttcac caatccctgg gatataaaac
      361 accccaggaa gtttatttag gtaaaactat aaacaaacag gaggaaataa tacaccttaa
      421 aagtctcttt tctgtcttga caagggggtc caccttatat taccactaca ctttggaaag
      481 aattaaatat aaatgcctgt atttactggg tttgagacat cagaataggt gaaaatcagg
      541 agaaagtgtc aaagttggta gaatcattta acattcttca caaaatttct taaattctaa
      601 agctgccttt tctacgtctt cctgaccgga gaaaatgctg gttgtcccag ctacaaaaac
      661 attagcccca gcgctcttta aaagaggaac ggttttttct ccaatccccc catccacggc
      721 aatatccacg ttcaaatctt tctcttctac catttctctt accctttgca ccttggggac
      781 aacttccgga ataaatttct gtcccacaaa tcccgggtct acagtcatta aaagcaccag
      841 gtctaaataa ggcaaaagat aagaaagaaa atcagggggt gtagaggggt tgagagccaa
      901 ccccactttt ttattttccc tttttactct atccactaac tgatgcaaac gactatctac
      961 ctcggcatga aaagtaatca tatctgctcc caaatcagca aataaggaga taaaagattc
     1021 aggattctgc accatgaggt gaacatcgaa aagaagagag gtagctggac gaagcttctt
     1081 taaaagatta gtacccagag caaaattagg gacaaacact ccatccataa tatcaatgtg
     1141 aagcatatct atttttgctc tttctaactt ttgtacttct tcccccaggt gaagataatc
     1201 agcacaatcc agagaagcag aaacttttat catagtttaa ccccctgagg ttctataacc
     1261 actttagtat aaggaaaacg tttttgctgc atttccaaca taaaggaagg agcttcttcc
     1321 aaacttatcc gatgagagat taatggggat aaagacactt ttcctctctc ttctaattcc
     1381 agtaaccttt cccagtctga atcaagagaa gaaaaatctg aattccaggt gcccagaatg
     1441 gtaagttcgt gacgaagaac gatgctgatc tcctgaggct ttaaaactac ttctccctct
     1501 tgatttccca ccaggagaac cttgcctttc tttctaacta tccctaaagc atccaaaaga
     1561 gctcgacgac ttcctgaagc ttcaatggct aaatccacct cctctttttc ccctgctgga
     1621 gataaagtta agtcaaaacc tgaagcacgc gctaagggaa atttttgctc atccacgtca
     1681 tatcctgtga gcattcgagc tccccctaac tttgcccata taccacagag aagcccgata
     1741 ggacctaaac caaaaactgc tactctatct cccatctccg gatgaacacg tcgcaaggca
     1801 tgcaaggtta ctgctcccgg ctcactcaac gctccttctt cccaggaaac acaggaagga
     1861 aggagaacaa gattttctac aggagctacc acaaactcgg caaacccccc attttgccga
     1921 gaacctaaat acccataatt atcacacaac tcgtattgac ccgcttcaca atatcggcaa
     1981 tttcggcaag ggatgagggg gtaaactgta actctttttc ctatccattc ttcatcctct
     2041 tttttcccta cttccactac ttcacctgaa aattcatggc caggaataag agggtaaaaa
     2101 taagcttgac caccaaaaac tctaggaagg tctgagccac atattcccgc tgcctgaact
     2161 cttatcagag cttccccttc tcgaggagca ggtgtttgta tctctcgcag ggataaaatt
     2221 cctttttctt ccagaactag agccttcatg ggttaaccac aactttcaac ccttcgtgag
     2281 attcggccac tctaaaagct tcctgaattt cttccagagg gaaacggtga gtaatatact
     2341 ccccggggct aaccatcccc ttttccatta cctctaaagc tttccggtgg tggcgaggaa
     2401 gactaccgtg actacccaat atcatacact cccgataatg aataaggttg ctgggaatac
     2461 tgatattacg cgctccagaa ggcaatcccc caaaaaaatt tattcgagcc cggtggcgag
     2521 ctaaaagaag agcttgctct tgagcatcgg gagaagcaca ggttatcatc accacatctg
     2581 ctccttctcc attggttact tcttttattt tttcttctac atttttatca ctcaaaaaat
     2641 aatggtcagc attaaattta cgagccagat ccagacgccg ttgagaacgc tggataacta
     2701 ttatattagt tgctcccata tgacgaccca tttctatcat catacaacca ataggacctg
     2761 ctccaataat acacagagca tctcccactc ctaagttagc catctccagc ccattaatgg
     2821 cacaggctaa aggttcagct aaagatgcct catcaaaaga aagtttttcc ggaattttat
     2881 gtactgaccc ttgttctaca gatagagcat taagtagaat atattgagca aatcctcccg
     2941 ggaattgata gccaatggcg tagtttatag cgcagttagt ccccattcca ttctgacacc
     3001 aaaaacattt tccgcaggga acatctgccc ctaccgctac tcggtctccc acctcaaatt
     3061 tttctactcc tttacccact tctatcacca cacctgcgat ttcatgacca ataatgtgag
     3121 gaaacttaac ccgagggtta ccagagtgca taatcctgat atctgagcca caaatcgcac
     3181 aggatttaac ttttaccagt acctcccctt ctctcacctg atagtcttct acttccttca
     3241 ccactaaccg gtctatatct tctaaaagag cagctttcat tgtaacctcc ttcttgtgga
     3301 ggaaaaccat tcctccctgt actattttta cagccaacgc cgagtatcta ttttttatag
     3361 ttaaccgcaa aatccattac aatgactatg agggggaaaa gaccccctta taaccccgaa
     3421 ctataaaggg gagaacccct ttataatccc ccaaaaacaa aaaaatacaa aaagaaaaag
     3481 actactgagg gagaaaacat ccctcagtgg ggtaaacccc cttataaccc ccaaaagcaa
     3541 aaaacaaaag gaagaagcaa aaaattaaag actgaaaaga caaaaaccaa ctgaggaaga
     3601 aaaaacgtct tcctcagtac ctacggcacc aaagacgaaa gcaaaataga tgaaagaaac
     3661 taaaaccaat tacactggag aaaagcactc cagcgttgag gaaggaaaaa catcttcctc
     3721 agtataaggg ggaaagaatt cccccttatg aaccctcttt ctttt
//
LOCUS       DAOSVE010000121         3763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_10107_length_3763_cov_71.448220, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000121 DAOSVE010000000
VERSION     DAOSVE010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10107_length_3763_cov_71.448220"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..507
                     /locus_tag="PK016_08030"
     CDS             115..507
                     /locus_tag="PK016_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HXL03041.1"
                     /translation="MEEKIFVIQEHWASHHHFDFRLEKDGVLKSWAVPRSIPLKAGEK
                     RLAIEVEDHSLDYADFEGEITEGYGKGKVAIWDRGYYFADKWEEDEILFELKGNKVKG
                     KYALIRTRSGKLNDNHQWLLIKRKEDAS"
     gene            504..1028
                     /locus_tag="PK016_08035"
     CDS             504..1028
                     /locus_tag="PK016_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HXL03042.1"
                     /translation="MTIQEVLEQALKMEEDGRQFYLEGYERVSSSLSKEVLKRLADEE
                     LVHIERIKEGYNKVTKGEAFAVENWGGIRKPTREYFDNVFTEARKNMNEIITPGAQEL
                     DVIQASMDLESKSHQFYVEKSKEVEDDKVREFLDFLASEEYRHYNLLFNTYEYLSNPT
                     SWYYREEKPIFEGG"
     gene            1058..1645
                     /locus_tag="PK016_08040"
     CDS             1058..1645
                     /locus_tag="PK016_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HXL03043.1"
                     /translation="MKIVAIMGSPREGGNSDVMLNAFLSSRDSRHHLEWIVPSRLNLG
                     FCRGCRFCEAMGRCILRDDMDGVGNKLLEADKVVLSSPVFFYGFPASLKALIDRTQVF
                     WSRKYKMGEKFKYKEGFLLAVGATRGEKLFEGIVLTTRYFFDAWGCEYKGGLFFRGFD
                     QKGAIGECQKCLEEIKEAGKSFLRSEDVTAPTEPS"
     gene            complement(1965..2186)
                     /locus_tag="PK016_08045"
     CDS             complement(1965..2186)
                     /locus_tag="PK016_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HXL03044.1"
                     /translation="MACVTPEGKPTESGKKILSALKSGATSPEEVAKISGLPLFRVRS
                     GLRDMVQADLVAEEEGKYKLTGKGGEFIE"
     gene            complement(2202..2852)
                     /locus_tag="PK016_08050"
     CDS             complement(2202..2852)
                     /locus_tag="PK016_08050"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013171683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HXL03045.1"
                     /translation="MEELREEVVSFPRVGEVAPSFEAVTTHGTLRLEDFKGSWLVLFS
                     HPADFTPVCTTEFIAFSQAYPEFQKRGVELLGLSVDSVSSHIAWARNIQEKTGVKVPF
                     PIIADLSKEVSVKYGMIHPGESKTETIRCVFIIDPNQVIRAILYYPLTTGRNISEILR
                     IIDALQTTDKYGVATPANWQPGEKVIVPAPTTVEGAEERLKEGYECVDWYLCKKSV"
     gene            complement(2875..3294)
                     /locus_tag="PK016_08055"
     CDS             complement(2875..3294)
                     /locus_tag="PK016_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HXL03046.1"
                     /translation="MDGVNKTKKTKQRETILEILKNTKSHPTADEIYQKARQEIPHIS
                     KGTVYRNLKLLAERGEIFKIDTEGTEARYDVRVESHYHFRCDSCGKVFDIDIPVNAEL
                     DKKAAQKTGLDITHHILEFRGFCSECEKKQLSPPPGR"
BASE COUNT         1131 a          709 c          798 g         1125 t
ORIGIN      
        1 tgtttgtcat tcctgagtgg ttctggagga gactgctttc tctcatctgt tgctacagaa
       61 gcaaagagta ttaaaaaaat caaaaaacat gttacaattt aaagggggcg aaaaatggaa
      121 gaaaaaattt ttgtgattca ggaacactgg gcttctcacc atcattttga ctttcgtctg
      181 gaaaaagacg gggtcttaaa aagctgggct gttcctcgct ccattccttt aaaggcggga
      241 gagaaaaggt tagccataga agtagaagac catagcttgg attatgctga ttttgaggga
      301 gagattaccg aaggttatgg gaaagggaaa gttgcaatct gggatagggg atattacttt
      361 gccgataaat gggaagaaga tgagatttta tttgagctga aggggaataa agttaagggg
      421 aaatatgctt taattcgcac tagaagtggt aaactaaatg acaatcacca atggttattg
      481 attaaaagaa aggaggatgc cagttgacca ttcaagaagt tttggagcaa gctcttaaaa
      541 tggaagaaga tggccgtcag ttttacttag agggctatga gagagtaagt agctctttaa
      601 gtaaagaggt gttgaaaagg ttagccgatg aggaattggt gcatatcgag cgcattaaag
      661 aaggatataa taaggtaacc aaaggagagg catttgccgt agagaattgg ggaggtatta
      721 gaaaacccac tcgagaatat tttgataatg tttttactga agctcgaaag aatatgaatg
      781 agattataac ccccggcgct caagagctgg atgtgattca agcctctatg gatctggaat
      841 caaaatccca ccaattctat gtagaaaaaa gtaaagaagt ggaggatgat aaagtacgag
      901 agtttttaga ttttttagcc tctgaggaat atcgtcacta taatctttta tttaatacct
      961 acgaatattt gagcaatccc acttcctggt attatcgaga ggaaaaaccc atttttgaag
     1021 gaggttgaaa actccccggg agagagaacc tgaaagtatg aaaattgtag ccattatggg
     1081 gagtcccaga gaaggaggaa atagcgatgt gatgttgaat gcttttcttt cctcgaggga
     1141 ctctcgccat cacttagaat ggatagtccc ttcccggctt aacctgggat tttgccgggg
     1201 atgccggttt tgcgaggcca tgggaagatg cattttaaga gatgatatgg atggggtggg
     1261 gaataaatta cttgaggctg ataaggtagt tttaagctct ccggttttct tttatggatt
     1321 tcctgcctct ctgaaagcgc taatagatag aacccaggtt ttctggtcta ggaaatataa
     1381 aatgggagag aaatttaagt ataaagaagg ttttctccta gcggtaggag ccaccagggg
     1441 agaaaaactt tttgagggaa tagttttgac caccagatat tttttcgatg cctggggatg
     1501 tgaatataaa ggaggcctct tcttccgggg ttttgaccag aaaggagcta tcggagaatg
     1561 tcagaagtgc ctggaagaga ttaaggaagc gggaaaaagc tttttgaggt ctgaagatgt
     1621 gacagcccct accgaaccgt cctgacaaac accggcagct tacggtgtaa gaaaaagaca
     1681 tagtggacca ttggcaatct accgggggtt attgcaagca ccaagagaga cattcttttg
     1741 gtaatttttc ttatactttt acgtttttct ttcaattttc ctgaggatga tagaaagttg
     1801 gcctccgcta aggaaaattt tcttcctcaa tagtataaat atttcctgaa acagaaaatc
     1861 gagagtagaa aaattttaaa gtcttaagct accttacatc gagcaaattg ccgggaggag
     1921 ttcgattttc tactccctcc cggcaaaatc aacttctctt ctatttattc aataaattct
     1981 ccaccttttc ctgtaagttt atacttccct tcttcctctg ccaccaaatc cgcttgaacc
     2041 atatctcgaa gcccacttct tactcggaat agaggtaagc cgctgatttt agctacctcc
     2101 tcaggagatg ttgccccaga ttttagggca ctaagtattt tctttcccga ttcagtgggt
     2161 ttaccttccg gcgttacaca ggccataaaa attctccttt ctcagacgct tttcttacac
     2221 agataccaat ctacacattc atatccctct ttcaaccgct cttctgcccc ttctacggtg
     2281 gtgggagcag gaactattac tttttctccc ggttgccagt tagccggagt agccacaccg
     2341 tatttatcgg tggtttgcaa agcatcaatt attctcaata tttcactgat atttctacca
     2401 gtagttaaag ggtaataaag aatagcgcga ataacttggt tagggtcaat gataaacacg
     2461 caccgaatag tttcggtttt actttctcca gggtgaatca tgccgtattt gacagatact
     2521 tccttactaa ggtcggcaat aatggggaaa ggaactttaa ctccggtttt ctcctgaatg
     2581 ttacgagccc aggcaatgtg agaggacaca ctatccacgc tgagccccaa gagttctacc
     2641 cctctttttt gaaattcagg gtaagcctga ctgaaagcaa taaactcggt agtacatacc
     2701 ggagtgaagt cggcggggtg agaaaataac accaaccagc tccccttaaa atcttccagc
     2761 ctcaaagtcc catgagtagt aacagcttca aaagagggag ctacctctcc cactcggggg
     2821 aaactgacaa cttcttctcg taattcttcc atcactcata cctccttttt atttttatct
     2881 ccccgggggg ggagataatt gttttttctc acattcagag caaaaacctc gaaactctaa
     2941 aatatggtga gtaatatcaa gcccagtctt ttgagctgct tttttatcca gttctgcatt
     3001 tactggtata tctatatcaa aaacttttcc acagctatcg cagcgaaaat ggtaatgact
     3061 ttctactcga acatcatatc ttgcctctgt tccttccgta tcaattttaa atatctctcc
     3121 tctttcagca agtaacttca aattacggta taccgtacct ttactgatat gaggaatctc
     3181 ctgccgtgcc ttctgataaa tttcatcggc agtgggatga gatttagtat tttttagaat
     3241 ttctaaaata gtctctctct gtttagtttt tttagtctta tttactccat ccattgtaat
     3301 aatagttctc attactattt tcacaaatta taaatgacct ttaatctttt gtcaaattat
     3361 agtgaggctg gatagcatca actcattgta ggagtatttt tatttttcct tctgttatta
     3421 gctttttaga ctctctccaa acacaaacag gaacaattca agacttacaa aggtggacaa
     3481 cttctaatac tctgtaaaag agactatctt ccaaacagtc taaaatatag ccgacactat
     3541 cgttggaggt tatcaaatta ctttttgccc tctgttagat tataatatat acaaagaaat
     3601 aataacagag agaaatgaaa tatggacgaa aagcgagcgt tgaaaataaa tctggatgac
     3661 ctttgcttgg caatggagaa tagctcctac gagtgtgatt attacctgga cctggaaaca
     3721 ggagagataa tatttatttc tgattatatg gatgatgcaa acc
//
LOCUS       DAOSVE010000122         3727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_10213_length_3727_cov_72.605664, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000122 DAOSVE010000000
VERSION     DAOSVE010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10213_length_3727_cov_72.605664"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..3662
                     /locus_tag="PK016_08060"
     CDS             15..3662
                     /locus_tag="PK016_08060"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXL03047.1"
                     /translation="MFRFRSQKKRRTPDTNRVKILLFFMLFVFSFSLTAEAQERIKVA
                     VLPLVGKDKEDAYWGYFLRDIIKMGLEGESSAEICDTLLSDDIVREARISWSDVLMPS
                     TAKIIGGRIGCSYLLTGSFRYRAMAGKERILVSARLYHVESDDYLDIPSEIFDLDNLQ
                     GLVEYVVGESLSFMGLDYKAPVYLPPLVIDNLLPLYRGVQKMEEALATYGEAQYPDKP
                     LWKEAFSLAEETIEKEPQYLDAYSYLAYMYRKTNWWAKEIETWDLYLQKLQERGEKID
                     SRQISQTYLRLAYSYFYQKNLNLARESLQKAVEVSPQWAEPYLLWARIEYEDGNMEEA
                     EKLYSKAFELDPSLKEAEYFAQMAEKAQIYGKSAYEAYVAGYQSFAAGNLERAEQYLR
                     EATRLNPEMKEAYYWLGRTLYDAGKLKEAEKAWEKVLEIDPFHSQARRFLDKTQQEVK
                     YGREALDYFRQGYDLYEEGRYEEAANLFRQALRVNPTFPDAHDYLARCYYRLGKTEEY
                     VREREESAELLEDSKDKAWQYYQMGYELFSWGEKEKAIQTLEKAVKENASLADAHLLL
                     GELYGEKGNWTKSKEHYTQALEGLEGESRGLALWGAAVASFQSGEWADALPLLNELVV
                     NYPYGEFIEEAEVVRMEVLVREGNYQEAQRSFQQFLVRFPDSSFLERARFWYASSFYQ
                     AKDWENAQKLLEEFLRLYPESSYRSSIIEMLGYTYRNMGKETEAQKYFAQLEGEEGAF
                     LTADAYYREKDWGKAISSLEDYLSHYPQGKFSLEARFKLASSYLEMGNPLKAQELIKG
                     WENKLQEQFKKDFLPFSIKLHAQREEWSEVKEEILRLEKETGQLEEEYSLLLALAYSN
                     LGEEEEAKNVLLKAGKNPEEILVSPEKEEIQEILDLMERGDYQSALSRLEAIKGEENP
                     LIGESTIDFLEGKCYYQLGDFSRAFNLLSRALGSPDESFKEEALFYLFNSAYRLEKWD
                     KVIEVYSQMQNKDDSSVRFRAALAHYYSGNYEESIPILRTLQNDINFSSSAASLLIEE
                     LFILKNYTECVKEAENFLTVFPQDSQRERVLYLASWALYYSGDIEKVKEFIKSYQTEF
                     PEGKYGEELVSLLADIYLSQGKTDEAIIILQDIEESSDSHRKLYTWYRLANAYLGKED
                     FAQALPYFERLFQAGQSEYQTAAGYWWGVCLEYLDRLEEAKEVYQAVIETGKEDQWVS
                     KAQERWDVLTR"
BASE COUNT         1138 a          612 c          905 g         1072 t
ORIGIN      
        1 aggagaagaa gagaatgttc cggtttcggt ctcagaaaaa gagacgaact cctgatacca
       61 atcgggtaaa aattcttctt ttctttatgt tatttgtttt ttccttttcc ctgacagctg
      121 aagcacagga gagaataaaa gtagctgttt tacctctggt gggaaaagat aaggaagatg
      181 cctactgggg ctactttttg cgggatatca taaaaatggg tctggaagga gaaagtagcg
      241 cagagatatg cgatacttta ctttctgatg atattgtaag agaggcacgg atttcctggt
      301 ctgatgtttt gatgccttct accgctaaaa ttataggggg aaggattggt tgctcttatc
      361 ttttgaccgg ttcttttcgt tatcgtgcga tggcagggaa agaaagaatc ttagtgagtg
      421 ctcgtcttta tcatgtggaa agcgatgatt atttagatat acccagcgaa atatttgacc
      481 tcgataacct gcagggtctc gttgagtatg tggtgggaga gtctttgtct tttatgggtc
      541 tggattataa ggcgccggtt tatcttccac ctcttgtgat agataacctt ttacctcttt
      601 atcggggagt gcaaaagatg gaggaggcgc ttgctactta cggagaggca caatatccag
      661 ataagcctct gtggaaagaa gctttttcct tggcagaaga aaccatagag aaagagcctc
      721 aatatttaga tgcttatagt tatcttgctt atatgtatag aaaaacgaac tggtgggcta
      781 aggagataga aacctgggat ttatatttgc agaagctaca ggagagagga gaaaagattg
      841 acagccggca aatttctcaa acctatcttc gcctggctta ttcttatttt tatcagaaga
      901 atttgaattt agctcgagag agtttgcaaa aagcggtgga ggtcagtcct caatgggctg
      961 agccttattt gctatgggcg agaatcgaat atgaagatgg taatatggag gaagcagaaa
     1021 aactttattc taaagctttt gaattagatc cctctcttaa agaggctgag tattttgctc
     1081 aaatggcaga gaaagcccag atttatggca aatcagctta tgaagcctat gtagctggtt
     1141 atcaatcttt tgctgctgga aatctggaaa gggcagaaca atatttgcga gaagctactc
     1201 gtctgaaccc ggaaatgaaa gaagcttact actggctggg aagaactctc tatgatgcag
     1261 gaaaattgaa agaggcagaa aaggcctggg agaaagtttt agaaattgat ccctttcata
     1321 gccaggctcg ccggttttta gacaagacgc agcaggaggt aaaatatggt agagaagcgc
     1381 tagattattt tcgccagggt tatgacctct atgaagaagg tcgttatgaa gaggcagcga
     1441 atctattccg ccaggcatta agagtcaatc ctacctttcc tgatgctcat gactatttgg
     1501 ctcgctgtta ttatcgcctg ggaaaaactg aagaatatgt tagagaaaga gaggaaagcg
     1561 ctgaactttt agaagactcg aaggataagg cctggcaata ttatcaaatg ggttatgagc
     1621 tattctcttg gggggagaaa gaaaaagcta ttcaaacatt agaaaaagca gtaaaagaaa
     1681 atgcttcttt agccgatgct catcttttac tgggagaact ctatggagag aaaggaaatt
     1741 ggactaaatc taaggagcat tatactcagg ctcttgaagg cttagaagga gaaagtcggg
     1801 gtttagctct gtggggagcg gcagtagcca gtttccaatc aggagagtgg gcagatgccc
     1861 tgcctttgct taatgagctg gtagttaatt atccttatgg tgaatttata gaagaagcgg
     1921 aagtggtgag gatggaagtt ctggtaagag aaggaaatta ccaggaagct caacgtagct
     1981 tccagcaatt tctggttcgc tttcccgaca gttctttttt agagcgagcc cggttctggt
     2041 atgcttcgag tttttatcag gctaaagact gggaaaatgc tcagaaatta ctggaagaat
     2101 tcctacgtct ttatccggaa agttcctatc gttcttctat catagaaatg ttaggttata
     2161 cttatcgaaa tatgggtaaa gaaacagaag ctcaaaaata ctttgcccag ttagagggag
     2221 aagagggagc ttttctaact gctgatgctt attatcgaga aaaagattgg ggaaaagcca
     2281 tttcttcctt ggaggactac ctttctcatt accctcaagg taaattttct ttggaagctc
     2341 gatttaaatt agcttcttct tacctggaaa tgggtaaccc tcttaaagct caagagctca
     2401 taaaaggatg ggaaaataaa ctgcaggagc aatttaagaa agattttctg cctttcagca
     2461 ttaagttaca tgctcaaaga gaagaatgga gcgaagttaa agaagaaatt ttacgtctag
     2521 agaaagaaac cggtcagctg gaagaggaat attctttgct tttagccttg gcttacagta
     2581 atttaggaga agaagaggaa gctaaaaatg ttctccttaa agcaggtaaa aaccccgaag
     2641 agatactagt aagccctgag aaagaggaaa tacaagaaat attagacttg atggaaagag
     2701 gagattacca gtcggctctc tcgcgcctcg aagctattaa aggagaggag aatcctctta
     2761 tcggtgaatc gaccatcgac tttttggagg gaaagtgtta ttaccagctg ggagatttct
     2821 cccgggcttt taacttgctc tcccgggctc tcgggtctcc tgatgagagt tttaaagaag
     2881 aagctctctt ttaccttttt aattctgctt atcgtctgga gaaatgggat aaggtgatag
     2941 aagtttactc tcaaatgcaa aataaagatg actcttcagt tcgttttcga gcggctttag
     3001 ctcattatta ttcaggtaac tatgaagaga gtattcctat tttgaggact ttgcagaatg
     3061 atataaactt ttcctcttcc gcagcttcgc ttttgataga agagctgttt atacttaaaa
     3121 attatactga atgtgttaag gaagcagaga actttttgac tgttttccct caggattccc
     3181 agagagaaag agtcctttat cttgcctcct gggcccttta ttattccggt gacatagaaa
     3241 aagttaaaga atttataaaa tcttaccaga cagaatttcc ggagggaaaa tatggagagg
     3301 aacttgtttc ccttctggcc gatatttatt tatctcaagg caagacagat gaagcgataa
     3361 taattttaca agacatagag gaaagtagtg actctcatcg gaaactttat acctggtata
     3421 gattagctaa tgcgtattta ggaaaggaag attttgccca agctcttcct tatttcgaac
     3481 gtttgtttca agcaggacaa agtgagtatc agactgcagc tggctactgg tggggagttt
     3541 gtttagagta tctggatagg ttagaagaag caaaagaagt ttatcaagcg gttattgaaa
     3601 ccgggaaaga agaccagtgg gtgtctaaag ctcaggaaag atgggacgtg ttaaccagat
     3661 gaaaggagag atggaggttc tctaactatg caaatttttt ctctgataat taaaggtgga
     3721 tacgtta
//
LOCUS       DAOSVE010000123         3656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_10451_length_3656_cov_66.054429, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000123 DAOSVE010000000
VERSION     DAOSVE010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10451_length_3656_cov_66.054429"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..790)
                     /locus_tag="PK016_08065"
     CDS             complement(29..790)
                     /locus_tag="PK016_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HXL03048.1"
                     /translation="MKKLLSLVILILVVLIGASSWAITADEILDEVEARTTKFETQKS
                     TAKMILVDSKGREEVREVVMYSKDEGEDVISMIVRFLSPAEVKNVTLLSVKGGETMYL
                     YMPAFKKPRRIASSEKSEKFMGTDFTYDELGGSYSREDYDSTLVEEAEEVYKLELIPR
                     DPESNYSKLLMEVDKEKFYFRKIEFYDKDGNLWKTLEVPEIQEEEGGSITVKKIDLTD
                     LKENHKTIIEMEEIEKDIPLPDNFFSVRTIQRPEL"
     gene            complement(796..3567)
                     /locus_tag="PK016_08070"
     CDS             complement(796..3567)
                     /locus_tag="PK016_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HXL03049.1"
                     /translation="MQKISQWIVKNAWLILIAVLIVTIFFAFQVVNVKFKDDVTEYLP
                     EDDPEISFYNSLSDKFASFQKKSMIVALEFDDLFIPHNLELLQEIVESIEKLSSVKGV
                     SALTNMPKVVATDFGIEVKEVVETLPQTPEEAKNLRNDLEKDKLIWGKMVTEDGKGTI
                     IAVSFYETVDEEKAVDEVRAVCRPYEGEAQITYFGLPIIMEQTSYDAQRTLKVLAPLA
                     GLLLLLILYWGFRSLQGVILPIFVALLASLWTLGLAALIGESLTIVSATLPVILLAMV
                     TAYGIHFINRYYEERSRIGGDDVTRITLQEVFVPISLSALTTMGGFISLLSADIKPIT
                     EFGLYSTLGIFFGFLLATFFLGAFFTVFTPRRLPRHFTREEEANPQDLISRFLLLLTR
                     GVTHHRNLIIAIIIAALVVLTVGIPRIQVETTVEEQIGANHPLTKLLKYFQERFGGTD
                     YNYLYLETQNVKDPFVLREIIRISRFAERYEAFSDPSSIADFICNLNEAVDGWEAIPD
                     TREKVENLWFFAQDNSFIKDRISEDGKATLIEFRARETTSRQLEEEVETLKSFLAQRP
                     QRVTAVPITENGAAQYLAKSVVDDLEIFGLSFSEREAVEKMVLQFIKQPLGDFMTFDE
                     EFAQKVTHDALLEIEDLGLEEEEVKKALLSYYSQPDSSLSSVLETDLGIIEDDALYLA
                     DVLDLSMERVARNNKANTLRQEIENLVGQTLDSDFDFVFYQILDEYVFLPQENGDILV
                     SYRLTGTPIIANHINNQLFTQQIESLILAFIIVYLLLLLQLRSIKKSLVAVIPILMTV
                     YTSFGLMGLLGIPLNTATLMVASIAIGVGIDYTIHFTSRWYLELREGSPNPLRTTLVN
                     TGRGIVLNSLSVSGGIYILTLSRIRMLRMFGDLVATVLLISMFYTLMVLPLLLHAAEY
                     GNNKNIERG"
BASE COUNT         1187 a          810 c          629 g         1030 t
ORIGIN      
        1 aaaattttac tcctcatagt ctttcacctc ataactccgg ccgctgaatg gttctcaccg
       61 agaaaaagtt atcaggaaga ggaatgtctt tttctatttc ttccatctcg atgatagttt
      121 tatgattttc cttcaaatca gtaaggtcaa tctttttaac tgtgatagag ccaccttcct
      181 cttcttgtat ctcgggaact tccagagttt tccatagatt gccatcttta tcataaaatt
      241 caatttttcg gaaataaaac ttctccttat ctacttccat cagtagcttg gagtagttag
      301 actccgggtc tcgaggaata agttccaatt tatacacctc ttcagcttct tccaccaggg
      361 tagaatcata atcttcccga ctatagcttc ctcccagctc atcataggta aaatcagtgc
      421 ccatgaattt ctcacttttc tctgaactgg caatccggcg tggcttttta aaagccggca
      481 tataaagata catagtctct cctcccttaa cgctgagaag ggtaacattt ttcacctccg
      541 ccggactcaa aaaacgaaca atcatagaaa taacatcttc tccttcatct ttagaataca
      601 taactacctc tcttacttct tccctacctt tagaatccac caaaatcatt ttagctgtcg
      661 atttttgagt ttcaaactta gtagtgcgag cttctacctc atccagaatt tcatccgctg
      721 ttatagccca ggacgacgca cctattaaaa ccaccagaat caatatcact aaagataata
      781 actttttcat tgaattcatc ccctttctat atttttatta ttaccatatt cagccgcatg
      841 cagaagcagt ggtaacacca tcaaagtata aaacatactg attaacaata ctgtagccac
      901 caggtcacca aacatgcgga gcatccgaat acgagaaaga gtaagaatgt aaattcctcc
      961 cgaaacagac agagagttaa gcactattcc tcttcccgta ttcaccaggg tggtacgaag
     1021 aggattggga gagccttccc tcagttctaa ataccaacga gaagtaaaat gaatggtata
     1081 atcaattccc actccgatgg cgatactggc taccatcaaa gtagcagtat tcaaaggaat
     1141 acctaacaat cccatgaggc caaaagaagt ataaacggtc atcaaaatgg gaataacagc
     1201 aacaagagat tttttaatag agcggagttg taaaagcaat aaaaggtaaa caatgatgaa
     1261 agccagaatt agactttcaa tctgttgagt aaagagctgg ttgtttatgt gattggcaat
     1321 aatgggcgtt ccggttaacc ggtaagaaac caggatatct ccattttctt gaggaagaaa
     1381 aacatactca tcaagaatct ggtaaaacac aaaatcgaag tcactatcca gggtttggcc
     1441 taccagattc tctatttcct ggcgtaaggt attggcttta ttattccgag ccactcgctc
     1501 catagataaa tctaaaacat cagccagata cagagcatca tcttcaataa tccccagatc
     1561 cgtctccaaa actgaagaaa gtgaggaatc cggttgagaa taataggaaa gaagagcttt
     1621 tttaacctcc tcttcttcta accccaaatc ttcaatttct aagagagcat catgggttac
     1681 cttttgagca aattcttcat caaaggtcat aaaatcgcca aggggttgtt ttatgaactg
     1741 gagaaccatt ttctctaccg cttctctttc agagaaagat aagccaaaaa tctctaaatc
     1801 atccaccaca gatttagcca gatattgtgc tgctccattt tcagtaatag gtaccgctgt
     1861 aactcgttga ggcctttgag ccaggaacga tttcaaggtt tcaacttcct cttctaactg
     1921 gcgagaagta gtttcccggg ctctaaattc tattaaagta gccttcccat cttcacttat
     1981 cctatcctta ataaaactat tatcttgggc aaaaaaccac aggttttcca ctttttcccg
     2041 ggtatcagga atggcttccc atccatccac ggcctcattc aaattacaaa tgaaatcagc
     2101 tatcgaagaa gggtcagaaa aagcttcata gcgttcagca aaacgagaga tacgtattat
     2161 ttccctcaaa acaaaggggt cttttacatt ttgtgtttcc agatacaaat agttataatc
     2221 cgtgccccca aaacgttcct ggaaatattt caacaattta gtaagagggt gattggctcc
     2281 tatctgctct tctactgtag tttctacctg aatacgagga atccccacag tcaagactac
     2341 tagtgccgct ataataatgg cgattatcaa gtttcgatga tgggtaaccc cccgggttaa
     2401 aagaagcaag aagcgactga tgagatcttg aggatttgcc tcttcctccc gggtaaaatg
     2461 acgaggcaaa cgacggggag taaaaacagt gaaaaaagct cccagaaaaa aggtggctaa
     2521 gagaaacccg aagaaaatcc ccagagtgga ataaagtcca aattcagtaa tgggtttaat
     2581 atcggcagaa agcaaagata taaacccacc catggtagtt aaggcactga gagaaatggg
     2641 aacaaaaact tcttgcagag taatcctagt gacatcatct ccccctattc tggatctttc
     2701 ctcataataa cggttaataa aatgaatgcc ataagcggta accatagcta aaaggataac
     2761 tggaagagtg gcagatacaa tggtgagaga ttccccaatt aacgccgcca atcccagagt
     2821 ccaaagagaa gctaaaagag caacaaaaat aggaagaata accccctgta aagaacggaa
     2881 accccagtaa agaatgagca acaataataa tccagcaaga ggggctaaaa cttttaaggt
     2941 tctctgagca tcataagaag tctgttccat tattatagga agaccaaaat aagttatctg
     3001 agcttcccct tcataaggac ggcataccgc tcgtacttcg tccactgcct tttcttcatc
     3061 tactgtttca taaaaagaaa ctgctattat agtccctttg ccatcttcag taaccatctt
     3121 cccccagatt aatttatctt tttctaaatc attacgtaaa ttttttgctt cttctggagt
     3181 ttgaggtaaa gtctccacta cttcttttac ctctatacca aaatcggtcg ctaccacttt
     3241 aggcatgtta gtcagggcag aaacaccctt taccgaagat agcttttcta ttgactctac
     3301 tatttcctgc aacagctcta agttgtgagg aatgaaaagg tcgtcaaatt ccagagctac
     3361 aatcatcgat ttcttctgga atgaagcaaa cttatcactc agagaattat aaaaagaaat
     3421 ctccgggtca tcttcaggaa gatactcagt aacatcatcc ttaaacttaa catttactac
     3481 ttgaaaagca aagaaaatag taactattaa aacagcaatt aaaattagcc aggcgttttt
     3541 aactatccat tgagaaattt tctgcataag catcttcctc cctaactgac cattcagtcg
     3601 aaaatttgct ttgtttatga taattgatga ccggtaactt ttcaagagct aaaagg
//
LOCUS       DAOSVE010000124         3590 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_10654_length_3590_cov_67.293918, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000124 DAOSVE010000000
VERSION     DAOSVE010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10654_length_3590_cov_67.293918"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..1333)
                     /locus_tag="PK016_08075"
     CDS             complement(74..1333)
                     /locus_tag="PK016_08075"
                     /inference="COORDINATES: protein motif:HMM:NF016083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HXL03050.1"
                     /translation="MRFRNRRVSCIIKEIIIAAKKGEPLAQVIKLVLVFTIILLSLRR
                     KINLGATLAGAGVLLLFLSSITPTKSLEIAISTFKDYSNLNLMANIVCITILGNVLSY
                     TGQLNRLVKSTQAIFKDPRLATVALPALVGILPMPGGAIFSAPLVEASRTSPDTEGEV
                     LTFINYWFRHIWEYFLPLYPGILLASSLSGLSLPWIVIHHLPFSFLAIIGGWLVIRKM
                     KVENSSDPPVKTNFFEIIQPFIPLIPPILGIFSGIPVWAGGIMGIILTVLLGKLSFSQ
                     VIPAMFKNFPWLIMIDVAGILIFKTAITQESVLQPVIDFMISQKMPVIILGLVLAFFI
                     ALISGLTAAFVGVSFPLILPLISVSLERALPLLYIGGYLGIIFSPTHLCLILSCRYFK
                     TDIEKVYRLLLWPSILVLSWGVIWYFI"
     gene            1451..3235
                     /locus_tag="PK016_08080"
     CDS             1451..3235
                     /locus_tag="PK016_08080"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HXL03051.1"
                     /translation="MKKFVLSFLVGWLCFCSVALATPEFRGIWIDARSIPLAEDDIRD
                     MVRRVHSANFNALLVESFYLGQTIYPSSFLASQGLPSQMSTFQEAGIDPLNIIVEEAH
                     NLSLQVHVWFDMFYVGLNEPGALLSRYPQWSAVNRDGSTGYQQGNNYFFWLCPAHPGV
                     REFYSQLFREVGENYPIDGIHLDYLRFPDPTLADTCYASEHCQKFKEKYGIDPREISP
                     WSQEALYREWNKMRAQSLTDFVAYISEELHHSLPHLIFSCAVKPQGFPEELNPRSLQD
                     WPHWAELGLFDVFIPMTYSSRPPEFKGLLLWINTFLSSTPFYSGIWGVNLPDSSIARE
                     INISREAKSQGVSIFAYPYLTGETLNFLSTGPFPEKVAPPTIEGLSAPRELPPQVKPI
                     SYYQENARQITALYTDIPIVPDGVLEKRWDSARWQTDFALITGEGKATEETQIALLYN
                     SQNLYVAFLFEKDPHEIISSVTQRDGPVFYDDSVEIFLDPDKSKSFYYQLVINLSGVQ
                     YDGSSLQGAAWNGNWKAGVSQENHLYSLEVAIPFSEIAKESPSSKEEWGINFYRNNLK
                     PEEFSAWSPTPGVYAAPSFFGTVEFSMP"
     gene            3378..3590
                     /locus_tag="PK016_08085"
                     /pseudo
     CDS             3378..3590
                     /locus_tag="PK016_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917193.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
BASE COUNT         1053 a          680 c          759 g         1098 t
ORIGIN      
        1 tttagaggta ttgcggcatg ccattttaat tttttgatta tctgtgtttt ttattacccg
       61 ctctatgctg ctactatata aaataccaga ttactcccca ggaaaggact aaaatactgg
      121 gccagaggag caatcgatat actttctcta tatcagtctt aaaatatcga caggaaagga
      181 tgagacagag atgggtggga gaaaaaataa ttcccaggta tcctccaata tagagaagag
      241 gaagtgctct ctcaagagag acggaaatga gaggtaaaat taaagggaaa ctaaccccta
      301 caaaagctgc cgtcaatcca cttatgagag caataaaaaa agccagtacc aaacctaaga
      361 tgattactgg cattttctga gaaatcataa aatctattac tggttggaga acgctttctt
      421 gagtgatggc agttttgaat attagaattc cagctacatc aatcattatt aaccagggaa
      481 agttcttaaa catagcaggg ataacttgag aaaaagataa tttgccgagg agaacagtca
      541 gaattatccc cattatgcct cctgcccaaa ctggaattcc tgagaatatt cctaaaatgg
      601 gagggattaa aggaataaaa ggttgaataa tctcaaagaa attggtcttt actggcgggt
      661 cagaagaatt ttctactttc atttttctaa ttactaacca acctcctata atggccagaa
      721 aggaaaaagg aagatggtgt attactatcc agggaagaga taaaccagat agagaagaag
      781 ctaaaagaat tcctggataa agaggaagaa aatattccca aatatggcga aaccagtaat
      841 tgatgaaagt caaaacttct ccttcagtat caggtgaggt gcgagaagct tctaccagtg
      901 gagcagagaa aatagcccca cctggcatgg ggaggattcc cactaaagca ggaagggcga
      961 cagtagctaa tcgggggtct ttaaaaatag cctgggtgga ctttaccaat cggtttagct
     1021 gaccggtata agaaaggaca tttcccagaa tggtaataca tactatatta gccattaaat
     1081 taagattgga ataatccttg aaggtgctta ttgctatttc taggcttttg gtgggagtaa
     1141 tggaagagag aaaaagcagt agaacacctg ctcctgcgag agttgcgcct aaatttattt
     1201 tacgtcgtaa agaaagcaaa attatagtga agaccagaac gagttttata acttgagcca
     1261 aaggttcgcc tttctttgct gctataataa tttctttgat tatacaacta actcttctat
     1321 ttctgaacct cacagttagc cacatcggtc attttctgtg aaggaaaaat acgggttata
     1381 aaaatcggat attaggagga aatttgattc cgaggtggca tttttaatct ctctttgaga
     1441 taatacaatt atgaaaaaat ttgttttaag ttttcttgtg ggatggcttt gtttttgctc
     1501 tgttgccctg gcaactcctg aatttcgagg aatatggata gatgcgcgga gtattccttt
     1561 agccgaagat gacattcggg atatggtgag gcgggtgcat tcggctaatt ttaatgctct
     1621 tttagtggag tctttttatt taggccaaac tatttatcct tcttccttct tagcttctca
     1681 aggtttacct tctcaaatgt ctacttttca ggaggccggc attgaccctt tgaacatcat
     1741 tgtggaagaa gcccataatc tttctttgca agtgcatgtg tggtttgata tgttttatgt
     1801 agggctcaat gaacccggag ctttactttc tcgctatcct cagtggagtg ccgtaaaccg
     1861 agatggaagc actggttatc agcaagggaa taactacttt ttctggcttt gtcctgccca
     1921 cccgggggtg agagaatttt actctcagct cttccgggag gtgggcgaaa attaccccat
     1981 agacgggatt catctcgatt atcttcgctt tcctgacccg actttagccg atacttgtta
     2041 tgcttcagag cattgccaga agtttaagga gaagtatggc atcgaccctc gagaaattag
     2101 cccctggagt caggaggctc tttatcggga atggaataaa atgcgggctc aatctttaac
     2161 tgattttgtg gcttacatct cagaagaatt acaccattcg ttgcctcatt taattttttc
     2221 ttgtgcagtt aagccccagg gtttcccgga ggagctgaac cccaggtcct tgcaagattg
     2281 gcctcactgg gcagaattag ggctttttga tgtttttatt cctatgactt atagttctcg
     2341 acctccggaa tttaagggct tgctgctctg gataaacact tttctttctt ctacaccttt
     2401 ttatagtgga atctggggag ttaatcttcc tgattcttct attgctcgag agattaacat
     2461 ttctcgagaa gcaaaatcgc aaggagtatc tatttttgct tacccttatc ttaccggcga
     2521 gactttaaac ttcttatcta ctggtccttt cccagaaaaa gttgctcctc ctactatcga
     2581 aggattatca gctccgcggg aacttccccc tcaagttaag cccatatcct attatcagga
     2641 aaacgctcgg caaattactg ctctttatac cgatattcct attgtacctg atggagtttt
     2701 agaaaagagg tgggactctg ctcgctggca aaccgatttt gctcttatta ccggggaagg
     2761 aaaagctact gaggagactc agatagctct tctttataac tctcaaaact tatatgttgc
     2821 ttttcttttt gagaaagacc cccatgaaat tatctcctct gttactcaga gagatggacc
     2881 agtattttat gatgattcag tagaaatttt cttggatccg gataagagta aaagtttcta
     2941 ctatcagctg gtaataaatc tatcaggtgt tcaatacgat ggttcttcct tacagggagc
     3001 agcctggaat ggaaactgga aagctggagt ttcgcaagaa aatcaccttt actctctgga
     3061 ggtagctatt cctttttctg agatagctaa agagtcacct tcctcgaaag aagaatgggg
     3121 gataaatttt tatcgcaaca acctaaagcc ggaagaattc agtgcctggt cacctactcc
     3181 gggtgtctac gccgctcctt ccttttttgg aactgtagag ttttctatgc cctaaacagc
     3241 ttttttagaa atgcttttat tctgtgatat tctgatgcct caaacccagt aaatacaagc
     3301 atccatattt tattcttttc aaagtgtagt ggcaataata tataaataaa atagctctaa
     3361 tgctatatat acttcacatt accactctct cttttgaaag tgttaaagct gatagtttga
     3421 gagattttgg cttcagtaaa gatgggaagt tcaaagaagt tcaggtagtg ctgggattat
     3481 taatcgactg tgacggtaga ccagtgggat atgaactatt ccctggtaat acctttgatg
     3541 gtaaaacttt agaagctgcc ttagataaac tggagaagcg tttcggtata
//
LOCUS       DAOSVE010000125         3573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_10716_length_3573_cov_70.776862, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000125 DAOSVE010000000
VERSION     DAOSVE010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10716_length_3573_cov_70.776862"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..603
                     /locus_tag="PK016_08090"
     CDS             100..603
                     /locus_tag="PK016_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like YkuK family protein"
                     /protein_id="HXL03052.1"
                     /translation="MISQDLHFYSPTRGWLSFKETVLSIFDFIKEEPQFHYRLIIGTD
                     SRETDDSPRAQLFVTAIIIHRVGWGARYFWRKTHIHSLYTIRDRIYQEALLSLQTSQK
                     LMELLNSFPDNHLSNYNVEIHVDVGNNGPTRSLIKEIVGMIESMGFVAKVKPDSFGAS
                     RVAHRHT"
     gene            843..1133
                     /locus_tag="PK016_08095"
     CDS             843..1133
                     /locus_tag="PK016_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HXL03053.1"
                     /translation="MKYNLYVIKNKNQKETNIWQSLGQVWDLAWVTVVPMILGLFLGR
                     YLDHNYPAGLSWTLSLLVFGSFMGFYNLYDSLMKESKKLEKKDKNKNGKGSN"
     gene            1114..1380
                     /locus_tag="PK016_08100"
     CDS             1114..1380
                     /locus_tag="PK016_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL03054.1"
                     /translation="MGKAVISGILGVVLAVIAIALLVRSVRKMVSSKKSPFWGEILGR
                     WALEAVFLFVFIKVFSLNIIVFLLSFSLSYFLLVWITSKTIFRG"
     gene            1385..2050
                     /locus_tag="PK016_08105"
     CDS             1385..2050
                     /locus_tag="PK016_08105"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HXL03055.1"
                     /translation="MEETLNPSVVFFIFGLPVTKIVVSTWFVMAFLVIASFISTRKLD
                     FIPTRFLQNVLELFVEAIIYLIEDMSPGNGKKFLPLVGTLALFIGTANLAGLVPGLSS
                     PTSDFNTTLALALVVFCSVPYYGIKTRGVKDYFKDYISPSPIMAPFHVISEITRTFSL
                     ALRLFGNILGEEIIIAILFLLSPLFVPVPMMLFSIFTGIVQAYIFTILTVVYLSAAVS
                     REN"
     gene            complement(2060..2581)
                     /locus_tag="PK016_08110"
     CDS             complement(2060..2581)
                     /locus_tag="PK016_08110"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HXL03056.1"
                     /translation="MIYFQLFLAFLRIGFFSFGGGMAALPLIEKEVVDTYQWLGSSEF
                     LELVTLSELTPGPIGINCATFTGFKVGGVLGAASATLAFCLPSILLVTLVFHFLSYFK
                     ENAIVQHFLRGLRPAVIALMAVAAYSLAQKGIKDWFAILLGIASFVFLFRKKTDPIFL
                     LLLSGAIGVIWYR"
     gene            complement(2578..3126)
                     /locus_tag="PK016_08115"
     CDS             complement(2578..3126)
                     /locus_tag="PK016_08115"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HXL03057.1"
                     /translation="MATLNRAKWATLGSLFFSFFKIGAFTWGGGYAMIPLIKREIVEK
                     KKMLTEEEFIEGLSVAQSLPGVMAVNTACFVGRKVEGMLGSIIAISGAVLPSFLIIML
                     IASFFLQYRHLEIVQNFFRGATPAIVALIAGGVADIGKSALDDWEDLLIAFFLFSLVI
                     FLKLHPLWIVLIGGVLGIARKK"
BASE COUNT         1024 a          689 c          714 g         1146 t
ORIGIN      
        1 taattttaac tttaattaag gaaaatagta aacaaaaata tgtttttagt gataaaatat
       61 aacgaaacta aagtgaggtg agttaaagac cgcgattcgg tgatttctca agatttgcat
      121 ttttacagcc ctacccgggg ttggttaagt tttaaagaaa cagttctttc catttttgat
      181 tttataaagg aagaaccgca gtttcattat cggttaatta ttgggactga ttctcgagaa
      241 acagatgatt ctcctcgagc gcagttgttt gttactgcca ttattatcca cagggtgggc
      301 tggggagcaa ggtatttctg gcggaaaact catattcata gtttatacac tatacgagat
      361 agaatttatc aggaagcact attgtcttta caaacttctc aaaaattgat ggagcttctg
      421 aattcttttc ctgataatca tctttccaac tacaatgtag aaatccatgt ggatgtagga
      481 aataatggtc ccactcgtag tttaatcaaa gaaatagtag ggatgataga aagtatgggt
      541 tttgtagcta aagttaagcc ggattctttt ggagcttctc gggtggctca tcgtcacact
      601 taaaaagagt gattgacttt cataccggat gatttttgtt accctcttct tttgtctttg
      661 cgagcttcgc ataacttcct tttggagagt cattatggtt ttagttttgt agcaatctgg
      721 actttctctc ctctatagac tcagaaaaga aaatctacca caggagatgg aaatacgact
      781 ctggcgggag agctgctttt ctctcttctc gagagcgaag aaaagatttt ctttaccaga
      841 ttttgaagta taatttgtat gtgataaaaa acaaaaatca aaaagagacc aatatctggc
      901 aaagtttagg gcaggtctgg gatttggcgt gggtaaccgt tgtcccgatg attctgggat
      961 tgtttttagg acgatattta gaccataatt atcccgctgg cctttcctgg acgctttctc
     1021 ttttggtttt cgggtccttt atgggttttt ataatcttta tgattctctg atgaaagaga
     1081 gtaaaaaatt agaaaagaaa gataagaata aaaatgggaa aggcagtaat tagcgggatt
     1141 ctgggtgtgg ttttagctgt gatagccatt gctctccttg tccggagcgt gagaaaaatg
     1201 gtatcttcta aaaaatcccc tttttgggga gaaattttgg gaaggtgggc tttagaagct
     1261 gtctttttgt ttgtttttat caaagttttt tctcttaaca tcatagtttt tttgctttct
     1321 ttttctttga gctatttctt actggtctgg ataacgagta aaactatttt tagagggtga
     1381 taagatggaa gaaactttaa atcctagtgt cgtctttttc atattcgggc taccagtaac
     1441 caaaatcgtg gtttctacct ggtttgtgat ggctttcctg gtaattgctt cttttatctc
     1501 tactagaaaa ttagatttta ttcctacccg atttttgcaa aatgttctgg agctttttgt
     1561 agaggctata atatacctta ttgaggatat gtcccccgga aatgggaaaa agtttctccc
     1621 tttagtggga acactggctc tttttattgg tacggctaac ttagcaggat tagtccctgg
     1681 tctgtcatcc cccaccagtg attttaatac tactttagct ttggctctcg tggtattttg
     1741 ctctgtccct tattatggga taaagacccg gggagtcaaa gattatttta aagattacat
     1801 atctccttct ccaattatgg ctccttttca tgttataagc gagattaccc gcactttttc
     1861 tctggctctg cgtcttttcg gtaatatctt aggggaagag atcataattg ctattctctt
     1921 tcttctctct cctctttttg ttcctgtacc tatgatgctt tttagcattt tcaccggtat
     1981 agttcaagct tatatattta ctattcttac tgtagtttat ctaagtgctg cagtgtcgag
     2041 ggaaaattaa atgctaaatt catctgtacc aaattactcc tattgctcca gataaaagga
     2101 gaagaaaaat ggggtcggtt tttttccgga ataaaaaaac aaaggatgct attcccagca
     2161 aaattgcaaa ccaatccttt atcccttttt gggccagaga ataagcagcc acagccatta
     2221 aggcaataac tgccggtcgt aaaccgcgca agaaatgctg tactatggca ttttctttaa
     2281 aataggagag aaagtgaaac actaaagtta ccagtaagat agaagggagg caaaaagcta
     2341 aagtagcaga agctgcaccc agtactcctc ctaccttaaa accggtaaag gtagcgcaat
     2401 taatcccgat gggtccggga gtgagttcag aaagagtgac gagctccaga aattcgctgc
     2461 tgcctaacca ctgatatgtg tctactactt ctttttcaat caaagggaga gcagccattc
     2521 ctcctccgaa agagaaaaat cctattcgta aaaaagcgag gaaaagttga aagtaaatca
     2581 tttttttctc gctatcccca gtactccccc gattaatact atccatagag ggtgaagttt
     2641 aaggaatatc accagggaaa aaagaaaaaa agcaatgagt aaatcttccc aatcgtctaa
     2701 ggcgctcttt ccgatgtctg ctactcctcc tgcaattaaa gctacaatgg caggagtagc
     2761 tccccggaag aaattctgta ctatttccag atgtcgatac tggagaaaaa aggaggcaat
     2821 aagcatgata attaaaaaag agggaagaac tgcccctgat attgctataa tgcttcctaa
     2881 cattccctct acctttcttc ctacaaaaca ggcggtattt actgccataa ctcctggcaa
     2941 actctgggct acggaaagtc cttcgataaa ttcctcctcg gtcagcatct tctttttttc
     3001 tactatttct cgcttaatta aaggaatcat agcgtatcct cctccccagg taaaagctcc
     3061 aatcttaaaa aaagaaaaaa agagagaacc aagagttgcc cactttgctc gatttaaagt
     3121 tgccatttcg acttcttaat ccccactttc ttgtcataaa gttgctgtag cgttctgccg
     3181 cataccggtg atattagtga atattaaaga tttatatact atattctcgc ccttgtaaat
     3241 agttcattca tccagaaacg aacaatagag gtagtttttg gtcttttgcc tttttcttcc
     3301 caggggttat aagctaataa tgtcccatag tttttctctg aacactacta tggttaaccg
     3361 caaaacctaa tataattgtt atcctcccct tccgtcatcg cgagggaggc tagaggccga
     3421 ccgtggcgat ctcgtttttc attgagtccc agcgaattcc gaaaggtaag gtacgtagta
     3481 ctaaaagctc gaggttgcca cgccaagcga aactgcgagg ctcgcgattg acctttggag
     3541 gggattacca tacccggcaa aaaccgctgt ctc
//
LOCUS       DAOSVE010000126         3514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_10925_length_3514_cov_66.683435, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000126 DAOSVE010000000
VERSION     DAOSVE010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10925_length_3514_cov_66.683435"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..714
                     /locus_tag="PK016_08120"
     CDS             28..714
                     /locus_tag="PK016_08120"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HXL03058.1"
                     /translation="MEKVILEDIPWEVEEENFLKKFHLKKGSEDAQRVRDLLKEAKKV
                     GHPRGMYHVAYIDKKGENEVTIEGITFNSKVLRTNLEDIYRVFPFVVTSGKELEEWSN
                     QFEDMLETFWVAEIKEWALKQAMHYMLENLKEKFHLDKVAAMNPGSLPDWPMEEQKKL
                     FVLLGNPEREVGVKLTDSYLMIPIKSVSGIWFPTKADFENCQLCSRENCPGRRAPYDE
                     KLTRKYQVKA"
     gene            complement(726..1739)
                     /locus_tag="PK016_08125"
     CDS             complement(726..1739)
                     /locus_tag="PK016_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HXL03059.1"
                     /translation="MPSIKEVARLSGVSIATVSRVINNKESVSKETAEKVWKAVRLLN
                     YQPNLLARSLRSQQSRLLGLLVPDIESPVFGRLAKYLEEAASKKGYSLIFCNTNDNPE
                     KEKKYLEILIQRQIEGIIFSRVLDESLLFKTPQLSKIPYVVLDRTLEKEEAPTIKLDN
                     YAAGALAASHFLNLGHKKFACLSGPLRIKICRERFYGFLDTLEKSGMSLREELIEEGD
                     FKMDEGRKAMNRILTVSLFEQPTAVFSMNDLMALGAIQAIKERGLSVPGDVSIIGLDN
                     IPLCDVISPPLTTVAQPFDEMAKEGINLLSKLIEGKKIRRTTIVVQPELVIRNSTAPP
                     RKR"
     gene            2020..3222
                     /locus_tag="PK016_08130"
     CDS             2020..3222
                     /locus_tag="PK016_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL03060.1"
                     /translation="MSNPASLLNIALEKGQGIFRLFPNWVPRAFCIPGKRLKLHPDDY
                     FAFGVHRGGIDERWFASTVRADNGPETLPDEGLSYIYVEDGSRVEKVLLKDAIELMGE
                     DILGKEVMKKYGGWTMFAKFFDNMDPLPHHLHLDDEKAAQVGRRGKPEGYYFPRQLNN
                     HGGYFPYTFFGLNSGTTRDDIIQCLKNWDKGDNGILYLSRAYRLKLGTGWNVPPGILH
                     APGSLLTYEPQGASDVSAMFQNIVWDRYIPWDMLVKDVPLEHKQDLDYIVDLIDWELN
                     LDPDFYNQHFFNPRPVKPLNEMRGEGYEEYWIAYNTEYFAAKELTIFPRHKVTIKEDA
                     SYGLIVIEGRGKFGALEIETPAIIRFGQATRDELFVTREAAINGITIVNESENENLVM
                     LKHLGPKP"
     gene            3361..3504
                     /locus_tag="PK016_08135"
     CDS             3361..3504
                     /locus_tag="PK016_08135"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL03061.1"
                     /translation="MVTGVYQKASGEVFYQGKLINFRISHDALQNGIATIYQEFNLIP
                     LYS"
BASE COUNT         1133 a          580 c          712 g         1089 t
ORIGIN      
        1 tatttaaagt ggaaggagta ggaaactgtg gaaaaggtaa ttttagaaga tattccttgg
       61 gaggtagaag aagaaaattt tttgaagaag tttcacttaa aaaaaggaag cgaagatgct
      121 caaagggtta gagatctatt aaaagaagct aaaaaagtag gtcatcctcg aggaatgtat
      181 cacgtggctt acatagataa aaaaggagaa aatgaagtta ctattgaagg gataactttt
      241 aatagcaagg ttttaaggac taacttagaa gatatttatc gagttttccc ttttgtggtt
      301 acatcaggta aagagttaga ggaatggtct aatcaatttg aggatatgct ggaaactttt
      361 tgggtagcag aaataaaaga atgggcattg aaacaggcta tgcattatat gctcgaaaat
      421 ttgaaggaga aatttcatct ggataaagta gcagctatga atcctggctc tttacctgat
      481 tggccaatgg aagagcaaaa aaagctcttt gttttactag gtaacccgga aagagaggtg
      541 ggagtaaagt taactgatag ttacctaatg attccaatta aatcagtttc gggaatctgg
      601 ttccctacca aggcagattt tgaaaattgc cagctttgtt ctcgagaaaa ttgtccggga
      661 agaagagctc cttatgatga gaagctgaca agaaaatatc aagtaaaagc ctgattaagg
      721 ttcatttatc gttttctagg aggagcggta gaatttctta tcaccaactc aggttgtact
      781 actattgttg ttctacggat ttttttacct tcaataagtt tcgagaggag atttattcct
      841 tctttagcca tttcatcaaa aggttgagct acagtagtta aaggaggaga aattacatcg
      901 caaaggggaa tattgtctaa gcctataata gaaacatctc caggaacaga taaacccctt
      961 tcttttatcg cttggatagc ccctagggcc attaaatcat tcatagagaa aacagctgtg
     1021 ggttgttcaa aaagagaaac ggtgagaatt ctattcattg ccttccttcc ttcgtccatt
     1081 ttaaaatctc cttcttcgat gagttcctct cttaagctca taccactttt ctccagagta
     1141 tccagaaaac catagaatct ctcccggcaa attttaattc ttaatggtcc agataaacag
     1201 gcaaattttt tatgacctaa gttcaaaaaa tgagaagcag ctaatgctcc agctgcgtag
     1261 ttgtccagct ttatagtagg agcttcttct ttttctagag ttctatctaa tactacataa
     1321 gggattttgg acaattgagg ggttttaaac aataaacttt catctaatac ccgggagaag
     1381 attattccct ctatttgacg ctgaattaga atctcaaggt attttttttc cttttccgga
     1441 ttatcgttag tattgcaaaa aattaaacta tatccttttt tagaagctgc ttcctctaag
     1501 tactttgcta atcgaccaaa aaccgggctt tcgatatcgg gaacgagaag accgagtaac
     1561 ctggattgct ggctacgcaa actccgggct agaagattag gttgataatt aagtaaacga
     1621 accgctttcc aaactttttc tgcagtctct ttgcttactg actctttatt atttattact
     1681 cgagatactg tagctataga aacccctgat agccgagcta cttctttaat tgatggcatt
     1741 ttattccctt ttgaaaacgt ttttataaaa caaaaaaatt tgtaagatgt atactatata
     1801 atatagtaaa attagttatt gacaaagtca agttattttg ttaacgtttt cagaaaacgt
     1861 taacaaaata acttgataga agcaaccaac aagattttta tggattttta tattagaatc
     1921 ttggcggcaa cgtaaaagag ttaataattt ttttgtttat gttaaagttt atgttaaact
     1981 tgtgatgtct ttaaaaagct acgaaaagga gatgaaataa tgtctaaccc agcctcgctt
     2041 ttgaatattg ccttagaaaa agggcagggg atttttcgtt tgtttcctaa ctgggttcct
     2101 cgagcatttt gtatcccggg aaaaaggtta aaacttcatc ctgatgatta ttttgctttt
     2161 ggcgtccacc gaggaggtat cgatgagcgt tggtttgcat ctacggtcag agctgataat
     2221 ggtccagaaa ccttacctga tgagggacta agctatattt acgttgaaga tggctctcga
     2281 gtagaaaaag tccttcttaa agatgctata gaactaatgg gtgaagatat attaggtaaa
     2341 gaggtaatga aaaaatatgg tggttggact atgtttgcca agttttttga taatatggat
     2401 cctcttcctc atcaccttca tctggatgac gaaaaggccg cacaagtagg gcgaaggggg
     2461 aaaccagaag gatattactt tcctcgtcaa ttaaataacc acggaggtta tttcccttat
     2521 actttctttg gtcttaattc aggaactact cgagatgata ttatccaatg tcttaaaaac
     2581 tgggataaag gagataacgg catcttatat ctttctcgag cttatagact aaaactagga
     2641 actggctgga atgttcctcc gggaattttg catgctcctg ggtctttgct tacttatgag
     2701 ccacagggag cttccgatgt ttctgctatg ttccagaata tcgtctggga tagatatatt
     2761 ccctgggata tgttagtaaa agatgttcct cttgaacata aacaagattt agattatata
     2821 gttgacctca tagattggga actaaactta gatcctgatt tctataacca gcacttcttt
     2881 aatcctcggc cggttaaacc tctaaacgaa atgagaggtg aaggctatga agaatactgg
     2941 atagcatata atactgagta ctttgcagct aaagaactta ctatattccc ccgacataag
     3001 gtaaccataa aagaagatgc atcttatggc ttaattgtaa ttgagggaag aggtaaattc
     3061 ggagctttgg aaatagaaac ccccgctata attcgttttg gccaagcgac cagagatgag
     3121 ctattcgtaa cccgggaagc agcaataaac ggtataacta tagtcaacga aagtgaaaat
     3181 gaaaacctgg ttatgttaaa acatttggga cctaaaccct gaaatagaag aggttatttt
     3241 tatggtttga taaactaact aaaaaattcc tgggatagtg gctccggatg aggttagttt
     3301 tgatattttg cgaggagaag tacacacgct ggtaggagag gtaaatcaac cttaattaaa
     3361 attgttaccg gggtttatca gaaggcatca ggagaagttt tctatcaagg gaaactcatc
     3421 aattttcgca tctctcatga tgccttacaa aacggaatag ctactatata tcaagaattt
     3481 aacctcattc cgctttatag ttaaccgcaa aatc
//
LOCUS       DAOSVE010000127         3472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_11104_length_3472_cov_76.431958, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000127 DAOSVE010000000
VERSION     DAOSVE010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11104_length_3472_cov_76.431958"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..590
                     /locus_tag="PK016_08140"
     CDS             93..590
                     /locus_tag="PK016_08140"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HXL03062.1"
                     /translation="MRKTWLLVALVSVLVLSIAFPIFAQGRSLAPYQERDRQMKQDRL
                     HQNISYSLNLTEEQKVQTLDIWAKYRSDIRDLQDQMEEARIALWKLSLETPSEEVASE
                     IRDKIGEINSWQQKIQEIRDTMFQEILSLLTPEQVSQLAKQYLPKGGRDFALQRGMGF
                     PENLR"
     gene            665..1342
                     /locus_tag="PK016_08145"
     CDS             665..1342
                     /locus_tag="PK016_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXL03063.1"
                     /translation="MAKILVVEDEESVRELLRLYLEKEGFQVELASDGEEALEKMSRI
                     SPDLVLLDLMLPKKDGWEVCREIRKTSYVPIIMLTARGEEFDKVLGLELGADDYVAKP
                     FSPRELVARIKAVLRRFNTSLGGGKTWEALGLKIDYEAKKVEIKGKEINFTPKEFELL
                     WFLVNHPQRVYTREQLLEYVWGYDFAGDARTVDTHIKRLREKLTEGGIASSIKTVWGY
                     GYKFEPG"
     gene            1339..3120
                     /locus_tag="PK016_08150"
     CDS             1339..3120
                     /locus_tag="PK016_08150"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXL03064.1"
                     /translation="MMKKTLFSKLLLSYLAVVAFSFLASGILSSQFFSRHYLASKEKE
                     IMEQAKTISEFISQSPQEEIPEILPRLRRGTQILLLSRQEFSSPNFPVSEAPRPGPGG
                     MRRETISKDKLEKVLQGETIVFRGFNPRFNQVMLMAASPVIQDGEVSSILLLASPIVS
                     IEATVSALRRIILMASAISLIFSLVLSYFFSRSLTRPLQKISEAARDLASGNLQRRIE
                     VHTEDELGQLAQDFNYLSSALEKTINHLHQEKRETENILLHMAEGVVAIDEEGKITSV
                     NPALLKKLKWEGEAPSGLHLSQLFPKSEIQEIFFRALKEGREEAQEFQIENGDHLILH
                     VTPLKEEEKVYGVVGVFQDITELRQLEELRRDFLANVSHELRTPLTSIQGFVEAIMDG
                     VISDEELKKKYLEVIHRETLRLSRLIHDLLDLSLMESKKIEWEVNPLSLSQMIDQVLL
                     KLMPQIEDKKIQIKKDFPPRIPLALGNTDRIEQVLINLLSNALIFSPPESIIEIKAWR
                     EEKEVAISIKNQGEGIPEKDIPHLFERFYRVEKSRSREKGGTGLGLAIVKQIIENHGG
                     TIRVESQSQEGTAFIFTLPIAPPPEGD"
BASE COUNT         1116 a          735 c          736 g          885 t
ORIGIN      
        1 caaactttca taaattggta acaaatcgct cactgaaaag ccaaaaagac cccttagaat
       61 attctcagaa aactcaaaag gaggtcagac acatgagaaa aacttggtta ctcgtagctt
      121 tagtgagcgt gcttgtttta agtatagcct ttccaatttt cgctcaagga agaagccttg
      181 ctccctatca ggaaagagac aggcaaatga agcaggatag attgcaccag aatattagct
      241 attctcttaa cttgactgaa gaacaaaaag tccaaacact ggatatctgg gctaaatatc
      301 gttccgacat ccgggactta caagaccaga tggaagaggc gcgcattgct ctctggaagt
      361 tgagcctcga aaccccttct gaagaagtag ccagcgaaat acgggataag ataggagaaa
      421 tcaattcctg gcaacaaaag atacaagaaa tcagagatac tatgtttcaa gaaatacttt
      481 ctctcttaac tccggagcaa gtctctcaac tggctaaaca gtatcttcca aaaggaggaa
      541 gagacttcgc cttgcaaaga ggtatgggct ttcctgaaaa cctcaggtaa gataattata
      601 gcagctccga agtaacttcg gagctgctat atcacacgat ataatgatta aaggtaaaca
      661 tggcatggct aaaattctgg tagtggaaga tgaagagagc gtacgagaac tcctccgcct
      721 gtatctcgag aaagaggggt ttcaggtgga attagcttcc gatggggagg aagctctgga
      781 gaagatgtcc agaatttctc ctgacctggt ccttttagac ctcatgcttc ctaaaaaaga
      841 tgggtgggaa gtatgccggg aaataagaaa aacttcttac gttcctataa ttatgctcac
      901 cgcccgggga gaagagtttg ataaagtact ggggttagaa ctaggagctg atgactatgt
      961 ggccaaacct ttcagtcccc gagagctggt ggcaagaatt aaggcagttc tccgccgctt
     1021 caatacctcc ttaggaggag gaaaaacgtg ggaagctctt ggcctcaaaa ttgattatga
     1081 agccaaaaaa gtggaaataa aaggaaagga aatcaacttc acccccaagg agttcgaatt
     1141 gctctggttt ttagttaacc atccccagag agtttacacc cgggaacaac ttttagaata
     1201 tgtatggggg tatgattttg ccggagatgc tcgaactgta gacactcaca taaaacgtct
     1261 ccgggaaaaa ttaaccgaag gaggaattgc ttcttcgata aaaacagtat ggggatacgg
     1321 ttataaattt gagcccggat gatgaagaaa actctatttt ctaaacttct cctttcctat
     1381 cttgcggtgg tagctttttc ctttttagcc tcagggatat tgagctccca atttttttcc
     1441 cgccattacc tcgcttccaa agaaaaggaa ataatggaac aagctaaaac catcagcgag
     1501 tttatttccc aatctcccca ggaagagata ccggaaattt taccccgtct gagaagagga
     1561 actcagattc tgctactcag ccggcaagaa ttctcctctc ctaattttcc tgtatcggag
     1621 gcacctcgtc ccggaccggg tggaatgaga agagaaacta tttcgaaaga caagctagaa
     1681 aaagtccttc agggagaaac catagttttc aggggattca atcctcgatt taaccaggta
     1741 atgttgatgg ctgcttcccc ggttatacag gatggagaag tctcctctat ccttctgctt
     1801 gcgtctccta tagttagtat tgaggctact gtgtctgcac taaggcgtat catccttatg
     1861 gccagcgcca tctctttaat tttttctctc gttctttcat atttcttttc tcgttctctt
     1921 acccgtcccc ttcaaaaaat aagtgaagca gcccgagatt tagcttccgg taatttacag
     1981 cggaggatag aagtgcatac cgaagatgaa ttagggcaat tagctcagga ctttaattat
     2041 ctttcttccg ctttagagaa aaccattaat cacctgcacc aggaaaaaag agaaacagaa
     2101 aatatactcc ttcatatggc agagggtgta gtagctatag acgaagaagg gaaaatcact
     2161 tccgttaatc cggctttact taaaaaactg aaatgggaag gagaagctcc ttcaggactt
     2221 catctttctc aactgtttcc taaatctgaa atacaggaaa tcttcttccg ggcgttaaaa
     2281 gaggggagag aagaagctca agagtttcaa atagaaaatg gagaccatct cattcttcac
     2341 gtaacaccct taaaagagga agaaaaagta tatggagtgg tgggagtttt tcaagacatc
     2401 accgagcttc gccagctgga agagctacgc cgtgactttt tagctaacgt ttctcacgag
     2461 ctgcgtactc ctcttacctc catccagggg tttgtagagg caataatgga cggagtaatt
     2521 agcgatgagg aactaaaaaa gaaatactta gaagttattc atcgagaaac actgcgcctt
     2581 tcccggctta tccatgacct tttagatctt tctctaatgg aatcaaaaaa aatagaatgg
     2641 gaagtaaatc ctttatccct ctcccagatg atagaccagg tattacttaa attaatgccc
     2701 cagatagagg ataaaaaaat ccagataaaa aaagatttcc ctccccggat tcctttagct
     2761 ctgggaaata ctgaccggat tgagcaagta ttgataaatt tactcagtaa tgctctgatt
     2821 ttttctcccc cggaaagcat catagaaata aaagcctgga gagaggaaaa agaagtagct
     2881 atttctataa aaaaccaggg agaagggatc cccgaaaaag acattcctca tctctttgag
     2941 agattttacc gggtggaaaa atctcgttct cgagaaaaag gaggaacagg cctcgggtta
     3001 gccattgtca aacaaatcat agaaaaccat ggtggcacaa taagagtgga aagccaatcc
     3061 caggagggga ctgcttttat ctttactttg cctatagccc ctccccctga gggagattaa
     3121 agcaaaacgg tcccccctat aacccccatt aaaaacataa aaagcaaaaa ctactgggaa
     3181 aggaaaagcg cctccctcag tggggcaaac tcctttataa ttcccttttt tcgtcacccc
     3241 agcaactttc gagtcgtttt tagcctgcct ctgaatagtt ctattatcac cttatctgtc
     3301 attcctgaat gtatttatca ggaatctaaa ctataaaggg gagggcccct ttataatccc
     3361 ccggaaagca aaagaaaaag acaaaaactt aaaaacccaa gactactgag gaagaaagaa
     3421 ccttcctcag tggattcccg ataaaaaatc tcgggaatga cgaaaagaga ag
//
LOCUS       DAOSVE010000128         3462 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_11147_length_3462_cov_63.009392, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000128 DAOSVE010000000
VERSION     DAOSVE010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11147_length_3462_cov_63.009392"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="PK016_08155"
     CDS             complement(<1..964)
                     /locus_tag="PK016_08155"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HXL03065.1"
                     /translation="MERILPATDENIKKGAKILKEGGLVAFPTETVYGLGANVFLPEA
                     VARIFEVKGRPRFDPLIVHISRHGMVSRLFREIPPLAEMLMEKFWPGPLTLVLPKKEE
                     VPDIVTAGLSTVAVRMPAHPVALKLIDWADVPVAAPSANRFGHLSPTQAEEVALDLGE
                     GVDLILDGGKTEIGVESTIIMIEEGKVFLLRPGGLSIEEIEKWVGKIETPPSSPKVLS
                     PGTLKKHYAPRIPLYLFEGKIEDLLLCDLSGIAIVSPFPIADSREEIKVLSEKGDIRE
                     VASNLFSVLRELEEGPFRAIISLTVEEKGLGRAIMDRLKRASSGY"
     gene            1044..1799
                     /locus_tag="PK016_08160"
     CDS             1044..1799
                     /locus_tag="PK016_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HXL03066.1"
                     /translation="MTGQSIILGLIQGLTEFFPISSSAHLILLRPIFNLGEQSLWFDV
                     LLHGGTWLAVLIYLIPYYSRAFRQPRIILNILLATIPAGLLGYLFEDIIENSLRNSFL
                     EICLLLVILGVIFLVVKERGNKTLENMTGKEALLIGVAQALALFPGVSRSGITLLAGF
                     LCGLQKKEAVLFSFFLSATTIGGAFILGIRNLSVSGNFLSPELVSGFLASFGSGLLAC
                     FLLLRIVKEKGLKPFGYYRIIFGGTMFIWFLFH"
     gene            complement(1796..2098)
                     /locus_tag="PK016_08165"
     CDS             complement(1796..2098)
                     /locus_tag="PK016_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL03067.1"
                     /translation="MIKLILEPVPYRYEETVYFYWEYELNSPYQLCFIPFRSLNKFLE
                     EMVRRETEIPESIHIILEDGRIKIWLPVAFYSEQLFNKVENILREKIRSLLEDVMF"
     gene            complement(2099..2734)
                     /locus_tag="PK016_08170"
     CDS             complement(2099..2734)
                     /locus_tag="PK016_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HXL03068.1"
                     /translation="MNESWELLSIFGSAFLIGFSGALMPGPLLALVLSGTYQVGYKAG
                     PLVVLGHGILELGLVIALMLGLGKVLDIPLVHQVIGIGGGVVLLYLGIDMLRMVRNSE
                     IFSVENSSNSEGGLAFKGLLVSLSNPYWIMWWATVGLALLLSATSFPFWGVVFFFLGH
                     ILSDFVWYSAVSFTLHRGKNFLSPRFYQWLVGICGGFMLFFGIYFISEVIR"
BASE COUNT         1091 a          792 c          619 g          960 t
ORIGIN      
        1 catatcccga agacgctctt ttcaaccggt ccataattgc tcttcccaat cctttttcct
       61 ctacggttag gctgattatg gcccgaaaag gaccttcttc caactctcgc aaaacactga
      121 agagattact ggctacctct cttatatccc ctttctcact caaaaccttt atctcttctc
      181 gggaatcagc aatcggaaaa ggagaaacaa tagctattcc cgaaagatca cacaagagga
      241 gatcctctat cttcccttca aaaaggtaaa gaggaatccg gggagcataa tgcttcttta
      301 aagtaccagg agaaagaact ttaggggaag aaggcggtgt ctcgattttt cctacccact
      361 tttcgatttc ttcaatactc aaccctcctg gccgaaggag aaagaccttt ccctcctcta
      421 tcatgattat tgtagattcc accccaatct ctgtttttcc tccatccaga atcaaatcca
      481 ctccttctcc taagtcaaga gccacttcct ctgcttgagt aggactcaga tgtccaaaac
      541 gattggcact gggagcagcc acagggacat ctgcccaatc tatcaacttt aaagctacag
      601 gatgagctgg catgcggaca gccacagtgg aaagaccggc agtaacgata tcaggaactt
      661 cctctttttt gggtaaaacc agagttaaag gaccaggcca gaacttttcc atcaacatct
      721 ccgcaagagg aggaatttcc ctaaagagac gggaaaccat cccatggcga gaaatatgca
      781 caataagagg atcgaaacga ggtcttcctt taacttcgaa aatacgagcc accgcctcag
      841 gtaaaaacac attagccccc agtccataga cggtttcagt ggggaaagct actaaccctc
      901 cctcctttaa aatttttgct ccttttttaa tgttttcatc tgtagccgga agaattcgct
      961 ccatttttct ccttttctcc tgatattgct ctctttttag ttctattata gaatagcaag
     1021 caaaaccgag gaaggagtca gctatgaccg ggcaatcaat tatattaggt ttaattcagg
     1081 gattaaccga gttttttccc atttcttctt ctgctcatct tattcttctt cgcccgatat
     1141 tcaatctggg agaacaaagc ctgtggtttg atgtgctgct ccatggagga acctggcttg
     1201 cagtattaat ttaccttata ccttattatt cccgggcttt tcgtcagccc cgcattattt
     1261 tgaatatcct cctggccact atccctgcag gattactggg atatttattt gaagatataa
     1321 tagaaaattc cttgagaaac agctttttag agatatgttt attattagtt attttaggtg
     1381 tgatattttt agtggtaaaa gaaagaggga ataaaacttt agagaatatg actggaaagg
     1441 aagctctctt aatcggggta gctcaagctc ttgctctttt cccgggggtt tcccgctcag
     1501 gtattaccct tctggctgga tttctctgcg ggctacaaaa aaaagaagcg gtgctttttt
     1561 ccttcttttt atcagctacc accattggag gagcatttat cttaggaatt agaaatttga
     1621 gtgtttcggg aaacttcctc tcaccggagc tggtgagtgg attcctggct tcctttggta
     1681 gcggattgct ggcttgcttt ctcctactga ggattgtgaa agaaaaaggg ctgaaacctt
     1741 ttggttacta tcgcatcatt tttgggggga caatgttcat ctggttttta tttcattaaa
     1801 acataacatc ttcgagaaga gagcggattt tctctctcaa aatattttcc actttgttaa
     1861 ataattgttc agaataaaag gcaaccggca accatatttt tattcttccg tcctctaaaa
     1921 tgatatggat actttcagga atttcagttt ccctcctcac catttcctct aaaaacttat
     1981 taagactcct aaagggtata aaacagagct gataagggct atttagttca tactcccagt
     2041 aaaagtaaac agtctcttca tatcggtaag gtaccggctc taaaattaac ttaatcattt
     2101 atcgaatcac ctctgagata aaataaatac caaagaaaag cataaaccct ccgcaaatcc
     2161 ccactaacca ctgataaaaa cggggagaga ggaagttttt acctcgatgc aaagtaaaag
     2221 aaaccgcgct ataccagaca aagtcagaga gaatatggcc aagaaagaaa aacaccactc
     2281 cccaaaaggg aaaagaagta gcagaaagaa gaagagccaa ccctacagta gcccaccaca
     2341 ttatccagta aggattagaa agactgacca gtaaaccttt aaaagccagg ccaccttctg
     2401 agtttgagct attctctaca gagaaaattt ctgaattacg caccatcctc aacatatcta
     2461 ttcccagata caataaaact acccctcctc ctattcctat aacctggtga actaaaggta
     2521 tgtccagcac tttccccaga ccaagcatta aagctataac caaacccagt tctaaaattc
     2581 catgacccaa aactaccaga ggtccagctt tatagcccac ctggtaagtc ccggaaagaa
     2641 ccagagccaa aagcggaccg ggcatcaatg ctcctgaaaa accaatcagg aaagcactgc
     2701 caaagataga taataattcc cagctttcgt tcatttcctt ttttttattt attatataac
     2761 aatcagcgag atatttactg gaaaaaactt aaaaataaaa tctaccgagg gagaaaaaac
     2821 ccctcaacaa aaaacttctg gattcccgat tgaagatttc ggtaatgacg taagaagatg
     2881 aacgcctcgc taaatttcag gcataataga cctttccttt tctcaccttg aaatcctctt
     2941 ttttattcgc ctcctgcact ttctccactg actcttttct ttctcccttc cgcaagagat
     3001 gagaaaagga gaagcccgca aggggtggga gaaagaaaaa aatcccacga ggatcgcaaa
     3061 aacagcctac ggcgccaaaa gcaaaagagc aaaaaattga aaccaaaaac taaaacaatt
     3121 acactggata atacgccaga atcatgatta caaaatggag agtgaattgc cttaaaaaag
     3181 tggcctgact cccttttaca aaaatttaga gcacttatcc acttactgca aaacaaaata
     3241 taggatataa tcgtctgtac tgaggtattt cccttagcta ttcatagagg aacttattta
     3301 cctggtacca atatataaaa cgatgagaaa acatatgcag acagaaatga aaaagttatt
     3361 atcatatctg aagccatact ggaaagcagc gctgttagct cctattctga tgctaataga
     3421 agtaatatgc gatttatctc aacccacttt attagccagc at
//
LOCUS       DAOSVE010000129         3430 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_11272_length_3430_cov_68.576889, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000129 DAOSVE010000000
VERSION     DAOSVE010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11272_length_3430_cov_68.576889"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            439..1329
                     /locus_tag="PK016_08175"
     CDS             439..1329
                     /locus_tag="PK016_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HXL03069.1"
                     /translation="MAIYYVGTCSWTDKTLIEEGSFYPPQFKNSAERLSFYAQHFNTV
                     EVDSTFYAFPAERNAHLWVERTPPSFLFNIKSFSLFTFHKTPLSALPLFLKEELPEKL
                     HNQPYLYLSDVPQEWLKMALSAFFSAINPLPEENKLGYVLFQFPPWIEKSKETMEYLS
                     WLRENTPGTLAIEFRHRSWLDEDNREETFRFLRQERLTYTCVDEPQLPWTVPPIVEAT
                     TAELVTRFHGRNKPAWEKKGASVEEKFAYLYQEEELNNWKEVVKKKSPDVERVFLMFN
                     NCFRDYAIVNAKQMKLFLTE"
     gene            1339..1959
                     /locus_tag="PK016_08180"
     CDS             1339..1959
                     /locus_tag="PK016_08180"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HXL03070.1"
                     /translation="MEIIDISLPLSEETPTYKGRKEKKPRLERIRTLSQGANETRLTL
                     ETHTGTHVDAPLHMLPSSPSIDQLPLSSFLGPALVAEIRGVETIALEHLSPFQQFLSP
                     RQFLILKTDNSFSDFSRENFVYLSTSGASYLAQQGIKGIGIDSLGIERDQPGHPTHRL
                     LLEKGILIFEGLYLREVEPGFYYFLSFPLKIKEGDGSPARAVLFRI"
     gene            1961..3328
                     /locus_tag="PK016_08185"
     CDS             1961..3328
                     /locus_tag="PK016_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL03071.1"
                     /translation="MKTLIFFGATGNLFTRKIFPALSSLFYRYEIRDLALIALGRRFS
                     EEEDYRRFLQEIHQREEKELLERIFYLPGDLENPEISLKISQLISKIGKEEVIFYLST
                     PPSLYKPALKTIEKTLSLSPPSVKKVALEKPFGSSEKDFEDLEKALKKNFREEEIFYV
                     DHYLGKATVQNLIILKAENLFIEKLLSRSFVKEVRIAVSEKEGIGERKKFYEETGAIK
                     DILQNHLLQLLTLLAIDIPPLCEEDKKECQRFLENVAQAKSQLLTKIQLPPQEAIYLG
                     QYQSYREEVGNPHSQVETLVYLPLYISSERWQGVPFRILTGKRMAERESYIEVVFHSY
                     SPEDNRLRIEIQPEERIDLFIRVKTPEMGLYSSPARLNFTYSGNFGANTPGAYEKIIY
                     DLLAGDRTLFPDSTFIRHAWQIVDQLISIIHKEKRNPHLYPEDTLTARDFWSSPPQLN
                     LSLPG"
BASE COUNT         1070 a          723 c          613 g         1024 t
ORIGIN      
        1 ttaatctgca aggtttttcc ctccctctca aactttctct agctcagtta aaaaagactt
       61 cttcaataat acaaatcggc aatttaagaa aaaactttat agagacttta aattttctcc
      121 tgactttgac acctttctct tctttttaaa ctctcctgac ctttaggacc actgagaaaa
      181 tctatattct gcttcttcta ttcaggatta gaaagtgaag taaagaaaaa cctatttact
      241 atctccacct ggtacaatcc tatagagatg aaccaggtaa gctcaaacat cgaataattt
      301 atgctttaga agagttatat tagtgatata atcaaattgc tgcgtttctt ctgcggtagc
      361 taaaataaag aagggtattt ttattgtgaa tgagattgta aagatataca atcgtataag
      421 ggaagggagg tgaaggtggt ggctatttat tatgtgggaa cttgttcctg gacagataaa
      481 accctcattg aagaaggctc tttttacccc cctcaattta aaaactctgc cgaaagatta
      541 agtttctatg ctcaacattt taataccgta gaagttgatt ccacttttta cgctttccct
      601 gcagagagaa atgctcatct ctgggtagag agaacccctc cctcttttct gtttaacata
      661 aaatcttttt ctctatttac cttccataaa actcccctat cagctcttcc tcttttctta
      721 aaagaagaat tgcctgaaaa gttgcacaac caaccctatc tctatttatc ggatgttccc
      781 caagagtggc taaaaatggc tctatctgct ttcttttctg ccataaatcc cttgccagaa
      841 gaaaataaac tgggttatgt tctctttcaa tttcctccct ggatagaaaa aagtaaagaa
      901 actatggaat atttatcctg gcttagagaa aacaccccag gaactttagc tatagagttt
      961 cgccaccgtt cctggttgga tgaggacaat cgagaggaga ctttccgctt cttacgtcaa
     1021 gaaaggttaa cctatacctg tgtagacgaa ccccaattac cctggactgt tcctcccata
     1081 gtagaagcca ctactgcaga gcttgtaact cgcttccatg ggcgcaataa accagcctgg
     1141 gaaaagaagg gagcatccgt agaagaaaaa tttgcctatc tttatcaaga agaggaatta
     1201 aataactgga aagaggtggt aaagaaaaaa tccccggatg ttgaaagagt tttcctgatg
     1261 tttaataact gttttcgtga ttatgcgata gtgaatgcta aacaaatgaa attatttctt
     1321 accgagtgag gtgaaattat ggaaatcatt gatatatctc tccctttgtc agaagaaaca
     1381 cccacttata aagggagaaa ggagaaaaaa cctcgtttag aaaggataag aactctttct
     1441 caaggagcta acgaaactcg cctgaccctg gaaacccaca ctggaactca tgtagatgct
     1501 ccccttcaca tgctcccttc ttctccctca atcgaccagc tacctctctc ctcatttctt
     1561 ggtcctgctt tagttgcgga aataagagga gtggaaacta tagctttaga gcatctctct
     1621 ccttttcaac aattcctatc tccccggcaa tttttgattc taaaaactga taactctttt
     1681 tctgactttt ctcgagaaaa ttttgtttac ctttctacat ccggtgcttc ttatttagct
     1741 caacaaggga taaagggtat aggaattgac tctttaggta tcgaacgcga ccagcctggg
     1801 catcccaccc atcgtcttct tttagaaaaa ggcattctaa tatttgaagg gttatatctc
     1861 cgggaggtag aacctggctt ttactatttt ctatcttttc ctcttaaaat caaagaggga
     1921 gacggttccc cggcaagagc tgtactcttt agaatataaa atgaaaacct tgatattctt
     1981 tggagctaca ggtaacctct tcacccggaa aatcttcccc gctttgtcct ctctcttcta
     2041 tcgctatgag ataagagatt tagctttaat agctttggga aggcgttttt ctgaggagga
     2101 agactaccgg agattccttc aagaaatcca ccagagagaa gaaaaagaat tattagaacg
     2161 aattttctat ctcccgggag atttagaaaa cccagaaatc tctctgaaaa tcagtcagtt
     2221 aattagcaaa atagggaaag aagaggtaat attttatctt tccacccctc cttctcttta
     2281 taaaccagcc ttaaaaacca tagaaaaaac cctgtcttta agtccgccct ccgtaaaaaa
     2341 agtagcttta gaaaaaccct ttggctctag tgaaaaagat tttgaagatt tggaaaaggc
     2401 tcttaaaaaa aattttcgag aagaagaaat tttttatgtc gaccattact taggaaaagc
     2461 caccgttcaa aatctcatca tcctcaaggc tgaaaatcta tttatagaaa aactgctctc
     2521 ccggtctttc gtaaaagagg tacgtattgc agtaagtgaa aaagaaggaa taggtgagag
     2581 aaaaaagttt tacgaagaga ctggtgccat aaaagacata cttcaaaatc atctccttca
     2641 acttctcact cttttggcta tagacattcc tcctctttgt gaagaagata agaaagagtg
     2701 tcaaagattt ctagaaaatg tagcacaggc caagagccag ttgcttacca aaattcaatt
     2761 acctcctcag gaagctatat acttagggca atatcaaagt taccgagaag aggtaggaaa
     2821 tcctcattct caggtagaaa ctttagttta cctaccctta tatatttctt ccgagcggtg
     2881 gcaaggagta ccatttcgaa tactgactgg aaaaagaatg gcagagagag aatcatatat
     2941 tgaagtggta ttccactctt attctccaga agataaccgt cttcgcatag agattcaacc
     3001 ggaggaaaga atagacctct ttataagagt aaaaacaccg gaaatgggat tgtattcttc
     3061 tcccgcccgg ttaaacttca cctattctgg aaactttgga gccaacactc ccggagccta
     3121 tgaaaaaata atatatgacc tcttagccgg ggatagaact ttgtttcctg attctacttt
     3181 tattcgtcac gcctggcaga tagtagacca gttgatttct attatccaca aggagaagag
     3241 gaacccccac ctctaccctg aagacacttt aaccgccaga gatttctgga gttcacctcc
     3301 acagcttaac ctaagtctcc ccggataaaa ttagctgatt agctagttct aactttgatg
     3361 gtttcttctt ttagttttac actgtaggca cccgaggaag tatttttctc ctccagtgct
     3421 cagcttttat
//
LOCUS       DAOSVE010000130         3381 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_11483_length_3381_cov_77.607637, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000130 DAOSVE010000000
VERSION     DAOSVE010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11483_length_3381_cov_77.607637"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..1529
                     /gene="gndA"
                     /locus_tag="PK016_08190"
     CDS             117..1529
                     /gene="gndA"
                     /locus_tag="PK016_08190"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HXL03072.1"
                     /translation="MEGFCDIGIVGLAVMGQNLALNMARKGFKVAVFNRTASRTRDFL
                     ETRVKDESIFPAYEVEEFLSLLASPRRVMLMVKAGQPVDDFIQLFAPHLSSGDLIMDG
                     GNSYYRDTDRRIKEAESKGFLYLGVGISGGEYGALHGPSIMPGGHREGYFLVKDILEK
                     AAAQVEDGPCCTYLGPSSAGHFVKMVHNGIEYAFMQSIAECYHLLREVERLSPPSIGQ
                     IFEEWNKGDDLNSYLVGITAQVLQYLDPDTGKPLVDLILDQAEQKGTGKWASQVALDL
                     GIPTPVINQSVIARNLSSFKEERKKISALFSEEKIEKSDVLSPQLEKALFLSELAAFC
                     EGFHMLQEASSEFSYNFNLSEIARIWKGGCILQARLLSQIQEALREEPDLLFTSPLFW
                     EKVKDCLPYLRQVCTLALQREIPVPALCASLSYFDSWKREWLPANLIQAQRDFFGAHT
                     YRRIDREGVFHTIWQKEENK"
     gene            1526..2800
                     /locus_tag="PK016_08195"
     CDS             1526..2800
                     /locus_tag="PK016_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HXL03073.1"
                     /translation="MILLEKGDAYSTLSSKDIEESLLPVLSSLPLENKKVLVIIPDST
                     RTIPMKLLFNILERALLGKTKKLDFMIALGTHPPMNDEELKRHLGVTYREAEERGIQI
                     FNHRWDKKEELREVGVLSSQEMEEISSFLIKEAVPVTVNKNIFNYDELIIVGPVFPHE
                     VVGFSGGYKYLFPGIAGEEITHKFHWFAALITNPKVIGHKNTPVREILNRAARFIPHP
                     ITLISLVMRGEEVCGLFAGDAQEAWSRAADLSSQVNIVYKDKPFSTVISVAPPLYNEL
                     WVGGKCMYKLEPVVADGGKLIIYAPHIKEISLTHGKYLREVGYHTRDFFLAQWEKYRH
                     YPGAVLAHSTHVKGIGKYVNGREYPRIEVILATGIPQEVCKEINLGYLDYRTLRPEDF
                     MGREEEGILVVPRAGEMLYKLSDGTVPDIDKL"
BASE COUNT          897 a          663 c          834 g          987 t
ORIGIN      
        1 tctcttaagg gaataataat ttaactttga tttttagttg aggcaactcg tgaggtagct
       61 cgttgggctt ttacatgcta aaatgatgat tattaaaagc aggaaggaga taaattatgg
      121 aaggattttg tgatattggc atagtgggct tggcagtgat ggggcagaac ctggctttaa
      181 atatggctcg taagggtttt aaagtagcgg tgtttaaccg cactgcgagt agaacccggg
      241 attttttaga aacaagagta aaagatgagt ccatatttcc tgcttatgag gtggaagaat
      301 ttctctccct ccttgcttct cctcgccggg tgatgctcat ggtgaaagcc ggacaaccag
      361 tggatgattt cattcaacta tttgctcccc atctttcctc tggagacctt ataatggatg
      421 gggggaattc ttactatcga gatactgacc gccggataaa agaggccgaa agtaaaggat
      481 ttctatacct gggggtggga ataagcggag gggaatatgg agctcttcat ggaccatcta
      541 ttatgcctgg aggacaccgg gaagggtatt ttctggttaa agatatcctg gaaaaagcag
      601 cggctcaggt ggaagatggc ccttgctgca cttatttagg cccttcatca gcagggcatt
      661 ttgtaaaaat ggtacacaat ggtattgaat atgcttttat gcaaagcatt gcggaatgtt
      721 accatcttct acgggaggta gagaggctct cccctccctc tattggtcag atttttgagg
      781 aatggaataa aggtgacgat ttgaactcct atcttgtggg aataacagct caggtcttac
      841 aatacttaga ccctgatacc ggtaaacctc tcgtggattt aattttagat caggcagaac
      901 aaaaaggaac ggggaaatgg gcttctcaag tagctctgga tctgggtatc cccacgccgg
      961 tcatcaacca atcagtgatt gctcgaaatc tttcttcctt caaagaagaa agaaaaaaaa
     1021 tcagcgctct tttttctgaa gaaaaaatag aaaagagcga cgttctttct ccacaattag
     1081 aaaaagctct ttttctctcc gagctcgcag ccttttgcga aggttttcac atgttgcaag
     1141 aagccagctc ggagttttct tataatttta atctatctga aattgcccgt atctggaaag
     1201 gaggatgtat ccttcaggct cgtcttctga gccagattca agaggcgctt agggaagagc
     1261 ccgatttact ttttacctct cctctttttt gggaaaaagt taaggattgc ttgccgtatt
     1321 tgcggcaagt ctgtactctg gctctccaga gagaaattcc tgttccagct ctttgtgctt
     1381 ccctttctta ttttgatagc tggaaaaggg aatggctgcc agccaacctc attcaggctc
     1441 aaagagactt ttttggagct catacttacc gcaggataga ccgggagggt gttttccata
     1501 ctatctggca aaaggaggaa aataaatgat tttattagaa aaaggagatg cttacagtac
     1561 tctttcttct aaagatatag aggaaagttt acttcctgtt ctctcttctc tccctctgga
     1621 gaataagaaa gttttggtga ttatccccga ttctactcgc actatcccca tgaagcttct
     1681 ctttaatatt ttagagagag ctctcttggg aaagactaaa aaactcgatt ttatgattgc
     1741 tctggggact catcctccta tgaatgatga agagttaaag cgacatttag gagttaccta
     1801 ccgggaggcc gaagagcggg gaatacagat tttcaaccat cgctgggata aaaaagaaga
     1861 actccgggag gtgggggttc tatcctcgca agagatggaa gagatttcct cttttcttat
     1921 taaagaggct gtgccagtaa cagttaataa aaatatcttt aattatgatg agctaatcat
     1981 tgttggtccg gttttccctc atgaagtggt ggggttttcc gggggataca aatacctttt
     2041 cccgggtatt gctggagaag agataactca taaatttcac tggtttgctg cccttatcac
     2101 taacccgaaa gtcatcggac ataaaaatac tccagtgagg gaaattttaa atcgtgctgc
     2161 tcgcttcatc ccccatccta ttacccttat ttccttggtg atgagaggag aagaagtttg
     2221 tggattgttt gcaggtgatg cgcaggaagc ctggtctcgg gcggctgatt tatcgtctca
     2281 ggtaaatata gtatataaag ataaaccttt ttctacggtt atatctgttg ctcctcccct
     2341 ttataatgag ctatgggtag gaggaaagtg tatgtataag ctggagccgg tggtagctga
     2401 tggaggaaaa cttatcatct atgcccccca tattaaggag atttcgctta ctcatgggaa
     2461 gtatctccgg gaagtgggtt accatacccg ggatttcttt ttagctcagt gggaaaaata
     2521 tcgccactat cccggggctg ttttggctca ttctacccac gttaagggga ttggaaaata
     2581 cgtcaatggt cgggaatatc cccgcatcga ggtaatactg gctacaggta tccctcagga
     2641 ggtgtgtaag gagataaatt taggttacct cgactatcgc actctccgtc cggaagattt
     2701 tatgggtcgg gaagaggaag ggattctggt agttcctcgg gcaggagaaa tgctttataa
     2761 actctccgat ggcaccgttc ctgatataga caagttgtaa acaatttatt actactgagg
     2821 gataagaagt tcatccctca gtagtttttg ttttttcttt tgtttacgcc gtaggctgtt
     2881 ctttcccgtc attcccgaaa tttagccggg aatccattga gggaggcttt ttctcccctt
     2941 agtaatcttg ggtttagtta agtttttatg tttttttgct tttctggggg ttatgagggg
     3001 gtctttttcc cctcatagtt gatagagaca ttcaggaatg acaacaaaaa aggggatggt
     3061 agaaccactc aggaatgacg gcaagaagac accctcaccc taaccctctc ccttcgaggg
     3121 cgaggggaaa aagatgtaaa gctctcccgt cgagggagag gggaaatatg taggttccgt
     3181 cgagggagag gaaaaactga gtctcccgca tttttttctc ccgcccctgg agggagggag
     3241 tgcccgggtt ccctgccaaa tcaacgattt ggcagggtga gcgagggagg gggaagttgc
     3301 tgaggaaaag tattttttga ggagaatcac cctcacccta accctctccc ttcgagggcg
     3361 agggaaggaa tatgtggggt t
//
LOCUS       DAOSVE010000131         3372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_11524_length_3372_cov_67.046126, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000131 DAOSVE010000000
VERSION     DAOSVE010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11524_length_3372_cov_67.046126"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..313
                     /locus_tag="PK016_08200"
     CDS             65..313
                     /locus_tag="PK016_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HXL03074.1"
                     /translation="MGIEELKNKTVVVGKRETERAINKNKAKKVFLAQDVDEEVQKEL
                     IDILKERGISWEYVPLAKDLGLACGIEIEASCAAIIED"
     gene            332..706
                     /gene="rpsL"
                     /locus_tag="PK016_08205"
     CDS             332..706
                     /gene="rpsL"
                     /locus_tag="PK016_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HXL03075.1"
                     /translation="MPTVNQLVRQGRKQSRKKSSAPALKNSPQKRGVCTRVWTITPKK
                     PNSALRKVARVRLTNGMEVTAYIPGIGHNLQEHSIVLIRGGRVKDLPGVRYHVIRGTM
                     DTAGVENRKQSRSKYGAKRPKS"
     gene            720..1193
                     /gene="rpsG"
                     /locus_tag="PK016_08210"
     CDS             720..1193
                     /gene="rpsG"
                     /locus_tag="PK016_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HXL03076.1"
                     /translation="MPRKGPVSKRKIPPDPVYNNVDVSCLINKIMRTGKKNVAEKIVY
                     GAFEVVRDKTKRDPMDVLSQALNNVMPLIEVRPRRVGGANYQVPMEVRPDRSKSLGIR
                     WLVSYAQGRPGRNMVDKLAQEIMDAANGTGGAVKKREDTHRMAEANKAFAHYRWF"
     gene            1227..3317
                     /gene="fusA"
                     /locus_tag="PK016_08215"
     CDS             1227..3317
                     /gene="fusA"
                     /locus_tag="PK016_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HXL03077.1"
                     /translation="MANRVQSESEEHLISKIRNIGIMAHIDAGKTTITERILYYTRKI
                     HRLGEVHEGAATMDFLPQERERGITISSAVTTCFWKDFRINIIDTPGHVDFTAEVERS
                     LRVLDGAVAVFSGLEGVEPQSETVWYQADHYHVPRIAFINKLDRVGADFYAAVQSIRQ
                     KLNANAHPLQVPIGKESNFRGVIDVIEMKAYLYNSEGEGTDYQTTEVPSELMSEAREV
                     RDKLVEALAELDDYIMEKYIEGEDPSPLEIREAIRRATIAGKFIPVLGGTALRNKAIH
                     PLLDAIVWYLPSPLDLPPIEGINPKTGEKEQRFASVEEPFSGLVFKIVTDPHVGKISF
                     FRVYSGSLKSGSYVYNPTKGEKERVSRLLMIHASHREDVDKIKAGDLCGIVGLRKATT
                     GDTLCDENYPIILESLSFPEPVISVAIEPKTRADQDKLTVALSKLSEEDPTFRVRTDE
                     ETSQTIISGMGELHLEIVIDRLLREFKVEANVGQPQVAYKETIKRSARAEGKFIRQTG
                     GRGQYGHVVLEVEPTKENFVFEDHIVGGTIPREYIPAVQAGVREALDNGVIAGFPVIG
                     VKVKLIDGSYHPVDSSDIAFKIAGAMAVKEAIQKAEPVILEPIMKVQVIIPQEYLGEV
                     IGDLNARRGKIEGMETRGAAQVVNALVPLKELFGYATALRSLTQGRGSYTMQFSRYEE
                     VPSFIKSEIIEKSR"
BASE COUNT         1058 a          539 c          863 g          912 t
ORIGIN      
        1 gactgttgac aggttcttca aatgctgcta aaattgctcc gttgcttttg ggggtgtaat
       61 ttttttgggc atagaggagt taaaaaataa aacagtagtg gtggggaagc gagaaaccga
      121 aagagcaata aataagaata aggcaaagaa agtttttttg gctcaggatg tggatgaaga
      181 agtgcaaaag gaattgattg atattttgaa agaaagagga atatcctggg aatatgtgcc
      241 cttagctaaa gatttggggt tagcttgtgg cattgaaata gaggcttctt gtgcagctat
      301 tattgaagat taaaagtaag ggaggaataa gatgccaact gttaatcaac tagtaaggca
      361 gggtagaaag cagtccagaa agaaaagcag tgctccagct ttgaaaaatt ctcctcaaaa
      421 aagaggagta tgtaccagag tgtggactat tactcctaaa aagcctaact cggctttacg
      481 taaagtagct cgagtgaggc ttactaatgg gatggaggtt accgcttata ttcctggtat
      541 cgggcataac ttgcaggaac actctattgt cctcattaga ggagggagag taaaggattt
      601 gcctggagtt cgttatcatg ttatccgggg gactatggat actgcgggag tggaaaatag
      661 aaaacaaagt cgctccaagt acggagctaa aagacctaaa tcttgaggag gtgagataat
      721 tgcccagaaa aggtccggtt tctaaaagaa aaatacctcc tgatccggtg tataataatg
      781 tggacgtgtc ttgtttaatt aataaaatta tgcgaacagg aaagaaaaat gtggcagaaa
      841 aaattgttta tggggctttt gaagtggtaa gagataaaac taaaagagac ccgatggatg
      901 ttttgtctca agctctaaac aatgtgatgc ctcttataga agtgagaccc cgccgagtgg
      961 gaggggcaaa ctatcaggtg cctatggaag tgaggccaga tcggagtaaa agtttgggta
     1021 tacgctggtt ggtaagttat gctcagggac gaccgggaag aaacatggtg gataagttag
     1081 ctcaagagat aatggatgca gctaatggaa caggtggagc agttaaaaag agagaagaca
     1141 cacatcgaat ggctgaggct aataaagctt ttgctcatta tcgttggttt taacaggagg
     1201 ccaggaagct taaggtgagt aaaactatgg caaatagagt acaatcagaa tctgaagagc
     1261 atcttatttc caaaattcgt aacattggaa taatggctca tattgacgca ggtaaaacta
     1321 caattaccga acgtattctt tattatacca ggaaaatcca ccgtctgggg gaggtccatg
     1381 aaggagcagc cactatggac tttctgcctc aggagcgaga gcgaggtata actataagtt
     1441 cggcagttac cacctgcttc tggaaagatt ttcgtattaa tattattgat acgccagggc
     1501 acgtggactt tactgcagag gtagaaagaa gcttgcgagt gttagatgga gcggtagcag
     1561 ttttctctgg attagaagga gtggaacctc aatcggaaac agtctggtat caggccgatc
     1621 actatcacgt acctcgtatt gcctttatta ataaattaga tagagtagga gcagattttt
     1681 atgctgcagt tcaatccatt cgtcagaaac tgaatgctaa tgctcacccc cttcaggttc
     1741 ctataggtaa ggaatctaat tttcggggag tgattgatgt aatagagatg aaagcctatc
     1801 tttataattc ggagggagaa ggtacagatt atcaaacaac tgaagttcct tctgagctca
     1861 tgagtgaagc ccgggaggtt agagataaat tagtggaggc tttagccgag ttagatgact
     1921 atataatgga aaaatacata gaaggagaag atccctctcc tcttgagata agagaagcta
     1981 ttcgacgagc aaccatagct ggtaaattca ttcctgtttt aggaggaacg gctctgcgga
     2041 ataaagctat tcatcctcta ctggatgcca tcgtctggta tcttccttct cctttggatt
     2101 tgcctcccat agaagggatt aatcctaaaa ccggggaaaa agaacagagg tttgcttcag
     2161 tcgaggagcc tttttctggc ctggttttta aaattgttac cgaccctcat gtagggaaaa
     2221 tatctttttt ccgggtttat tctggttcct tgaaaagtgg tagttatgtc tataatccta
     2281 ctaaaggaga aaaagaaaga gtaagccggt tattaatgat tcatgccagt catagggaag
     2341 atgtggataa aataaaggcg ggagatttat gtggcattgt gggattaagg aaagcaacta
     2401 ccggtgatac tctctgcgat gagaattacc ccatcatttt agaatctctt tccttccctg
     2461 agccggttat ctcagtggct attgaaccga aaaccagggc ggatcaagac aaattaaccg
     2521 ttgctctttc caaattatct gaagaagacc ctacttttcg agtgcgtact gatgaagaga
     2581 cttcccagac tataatttcg ggaatgggag agttgcatct ggaaattgtg attgaccgtc
     2641 tgttacggga atttaaagtg gaggcaaatg ttggtcagcc tcaggtggct tataaagaga
     2701 ctattaaaag atctgcgaga gcagagggta aatttattcg ccagaccggt ggaagaggac
     2761 aatatggtca tgtggtttta gaagtagagc ccacgaaaga aaattttgtt ttcgaagatc
     2821 atatagtggg aggaactatt cctcgagagt atattcctgc tgttcaagcg ggagtgaggg
     2881 aagctctgga taacggtgta atagctgggt ttccagttat aggggttaag gtaaaattaa
     2941 tagatggttc ttatcacccg gtagattctt cagatatcgc ttttaagatt gctggagcta
     3001 tggcagtgaa agaagctatt cagaaagctg aaccagttat tttagagccg attatgaaag
     3061 ttcaggttat cattcctcag gaatatttag gagaagtgat aggagatttg aatgcccggc
     3121 gagggaaaat tgagggaatg gaaacacgag gagcggcaca ggtcgttaac gctttagttc
     3181 ctcttaagga gctgtttggc tatgctactg ctttgcgctc tcttactcag ggaagaggta
     3241 gctacaccat gcagttttct cgttatgaag aagttccctc ttttattaaa agtgaaatta
     3301 tagaaaaaag tagatagagg gaggatagat gaattatggc aaagcaaaag tttgaaagaa
     3361 agaaacctca tg
//
LOCUS       DAOSVE010000132         3254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_12064_length_3254_cov_64.003126, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000132 DAOSVE010000000
VERSION     DAOSVE010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12064_length_3254_cov_64.003126"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="PK016_08220"
     CDS             complement(<1..515)
                     /locus_tag="PK016_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HXL03078.1"
                     /translation="MFFLVLFSGSAFALELRDLFWRADGETIKLTFKFNGSTNWVESS
                     PDPYHIVVDIEGATNSLGYNQRPMNIGPLRNIMFVEKDGVLRLWVEIREKVEYEIYDE
                     AGGRLLSIVFHLPPGGGEQGLFSFDFRDAEVRDVLLSLAKAAGVNIVVDDSVTGTISV
                     SFENLTFDQALG"
     gene            complement(536..1099)
                     /locus_tag="PK016_08225"
     CDS             complement(536..1099)
                     /locus_tag="PK016_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL03079.1"
                     /translation="MRSRDFYWMLVLVASLGFLAFLGFNFWKTLSSGEESDLFALTPV
                     SSPAVSSSSLEEIKEEELFLASYPEGELADFRNLFQSAIAAPWEVEKETVAVLPEESE
                     KEVVYIPSQEVIPAQEEVEEEIPPLHLQGVVVSGPRQAVIVEVEGKIQILTNEKNLSS
                     DIKLVKVEDKEVVLNYEGREVTLTFEN"
     gene            complement(1096..1659)
                     /gene="pilO"
                     /locus_tag="PK016_08230"
     CDS             complement(1096..1659)
                     /gene="pilO"
                     /locus_tag="PK016_08230"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HXL03080.1"
                     /translation="MNMSLEKNWPIFLLVWAGLLIGFFYLILIPGGEKLESLREEKGS
                     LGVQVVSLERKVRNLEGLERRLTELRSAASLLEERLPEEKEISNLLLVIEDSALRSGV
                     EIQSLKPQPLETQKDSDKPQPDYLEALFESNLMSDYHGFLLFLNYLRQSPRLIQVKNF
                     NLEEDQDNNLKIAMKLSTYVFSKRETK"
     gene            complement(1656..2210)
                     /locus_tag="PK016_08235"
     CDS             complement(1656..2210)
                     /locus_tag="PK016_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL03081.1"
                     /translation="MELKSIYPVKINLLPRRYIIKRTPNWARLFFIVVLIFFSFLTFF
                     FYINLNVKTSSLESDVESLEMRLVNLREREKRMEEVQTQINVIEERINTLKYLIQSEP
                     DWLKIMYTLGGCMPSDLHWDKASFAPSSFNCEGQVYSIFSLARFISSLDQHRDLFPEV
                     DFNSLALGEEGIYRFELSGRFKTP"
     gene            complement(2201..3178)
                     /gene="pilM"
                     /locus_tag="PK016_08240"
     CDS             complement(2201..3178)
                     /gene="pilM"
                     /locus_tag="PK016_08240"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HXL03082.1"
                     /translation="MLGVDLGAYSVKFCLTRKRGENFEPVFLGEKFFAPQSLEGGKIK
                     DRNALVDNLQQFCQRNHLPREVAVAFYHPRMVVQNISLPEMPDEELENALRWEASSII
                     TGENDFQIGWQTLGKEEGQQKILFAASPSLIVEDYLDVFRRARFEVEVFEPQMLSLIR
                     GFLSLHPDLVNSSFSIVDLGFTKGGILYFSNGKLLFSRYFNWGLGKAWSYLEEKFKLL
                     PTEIMELFDRSTKEGNIPYQMQETLSEVSKDLVTELRRSLSFLQSEFGSSSNQKLFLG
                     GGGARISFLRQILAGSLPVTIGEIPPLVVKKEEFPAEVYFSALGASLWS"
BASE COUNT          989 a          784 c          572 g          909 t
ORIGIN      
        1 cccagagcct ggtcaaaagt gaggttttca aaagaaacac ttattgtccc ggtaactgaa
       61 tcatccacca ctatattcac tcctgctgct ttggccagac tgagaaggac atctctcact
      121 tcggcatccc tgaagtcaaa ggaaaaaagc ccttgctctc ctcccccagg aggtaagtgg
      181 aaaactatac tcaaaaggcg accaccggct tcatcatata tttcatactc cactttttcc
      241 cggatttcta cccacaggcg aagaacacca tctttttcta caaacataat attccggaga
      301 ggtccaatat tcataggcct ttgattatag ccaagactat ttgtagctcc ttcaatatcc
      361 accacaatat ggtaaggatc aggagaagat tctacccagt ttgtactccc gttaaactta
      421 aaagtaagct tgatggtctc cccatctgct ctccagaata aatcccggag ttccagagcg
      481 aaagcgctcc ccgaaaaaag aaccagaaaa aacaaaatta tagttaccgt ccgggttaat
      541 tttcgaaagt taatgtaact tccctcccct catagtttaa tactacttct ttatcctcta
      601 ctttaaccaa ttttatatca gatgacaagt ttttttcatt ggttaaaatc tgaattttac
      661 cctccacctc cactatcact gcctgccggg ggccggaaac tactactcct tgcagatgaa
      721 gcggagggat ttcctcttcc acttcttctt gagccgggat aacctcttga gaagggatat
      781 aaactacctc tttttcagat tcttcgggta aaactgccac tgtctctttt tctacctccc
      841 aaggagcggc aattgccgac tgaaataaat tccgaaaatc ggccagttct ccttcaggat
      901 agcttgcaag aaatagctct tcttccttga tttcttccag actcgaagag gaaacagctg
      961 gggaagaaac aggagttaaa gcaaaaaggt cactttcttc cccggagctc aatgttttcc
     1021 agaaattaaa tcccaaaaaa gccagaaacc ccagactcgc tactaaaact agcatccagt
     1081 aaaaatctct acttctcact ttgtctccct tttagaaaat acataagtag aaagtttcat
     1141 ggcaatcttt aaattgttat cttggtcttc ttccaggtta aaattcttca cctgaattaa
     1201 acgaggagat tgacgaagat agttgagaaa aagcaaaaat ccgtgataat cactcataag
     1261 attactctca aatagtgcct ctaagtaatc aggctggggt ttatcagaat ctttttgtgt
     1321 ctcaagaggt tgaggtttta aagactgaat ctctaccccg gaacgcagag cggaatcttc
     1381 aatcactaga agaaggttag aaatttcctt ctcctctggt agccgttcct caagaagcga
     1441 agcagctgag cggagctcgg tcaacctcct ctccagtcct tctaaatttc tcacttttct
     1501 ctccagagaa accacttgaa cccccaaact tcctttctcc tcccttaaac tttccagctt
     1561 ttccccccca ggaattagaa tcaggtaaaa aaaacctatc aataatcctg cccagaccag
     1621 aagaaaaatt ggccagttct tttccaaact catattcatg gggttttaaa cctccctgat
     1681 agctcaaaac gataaattcc ctcttcccca agagctaaag agttaaaatc aacctccggg
     1741 aaaaggtccc ggtgctggtc aagggagcta ataaaccggg ctaaactaaa tatactgtaa
     1801 acctgcccct cgcagttaaa agaagaaggg gcaaaactgg ctttatccca gtgaagatcg
     1861 gagggcatac atcctcccag ggtatacatg attttcagcc aatcgggctc actttgaatc
     1921 aaatatttaa gagtattaat tctttcttct attacattta tctgagtctg tacttcctcc
     1981 attctttttt ctctctctcg gaggttgact aacctcattt caagagattc cacgtcgctc
     2041 tctaaagatg aggttttaac gttgaggtta atgtaaaaga aaaaagtgag aaaggagaaa
     2101 aaaattaaaa ccactatgaa aaataaccgc gcccagttag gagttctttt gattatatat
     2161 cgacgaggta aaaggttaat tttaactgga taaattgact tcaactccat aacgatgctc
     2221 ctagagcact gaaataaact tccgccggaa attcttcttt tttcaccaca agagggggaa
     2281 tttctccgat ggttacaggt aaactcccag ctaaaatttg ccggagaaaa gaaatccgtg
     2341 ctcctccccc acccaaaaaa agcttttgat tggagctcga gccaaactca gactgtaaaa
     2401 aggagagaga ccttctgagt tcagttacca ggtctttact cacctccgaa agtgtttcct
     2461 gcatctggta cgggatattt ccctctttag tgctccgatc aaaaagttcc attatttctg
     2521 tcggcaaaag tttaaatttc tcttctaaat aactccatgc tttgcccaga ccccagttaa
     2581 aataccggga gaagagcagt tttccattag aaaagtagag aatacctcct ttggtaaaac
     2641 caaggtctac tatggagaaa gaagaattca ccagatcagg atgcaagctc aaaaaccctc
     2701 gtatcaggct taacatttga ggctcaaata cttctacttc aaaacgagcc cggcgaaaaa
     2761 catccagata atcttccact atcagagaag gagaagcagc aaagagaatt ttttgctgcc
     2821 cttcttcctt acccagagtt tgccagccaa tttgaaagtc gttctctccg gtgatgatgg
     2881 agcttgcctc ccaccgcaga gcattttcca gctcctcatc aggcatttca ggaagagata
     2941 tattctgcac taccattcgg gggtgataaa aagctaccgc tacttcccgg gggagatgat
     3001 tcctttggca gaattgttgc aaattatcta ccagtgcatt tctatctttg atttttcccc
     3061 cctccagaga ttgaggagca aagaatttct ctcccaaaaa gaccggctcg aaattctctc
     3121 ctcttttcct ggtcagacaa aacttgacgg aataagcacc taagtcgaca cccagcataa
     3181 cagtctcctc aaaaaagact tagatattat tttatcataa attatttttt gctaaaacta
     3241 ccttttatta ttgg
//
LOCUS       DAOSVE010000133         3149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_12540_length_3149_cov_75.515514, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000133 DAOSVE010000000
VERSION     DAOSVE010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12540_length_3149_cov_75.515514"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="PK016_08245"
     CDS             complement(<1..681)
                     /locus_tag="PK016_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HXL03083.1"
                     /translation="MLRRDKVDQSFIRRKAEEERVPVVDFLLHKMKELEKIEGKPSSL
                     FAACPNSETVIKAALRSAKRANAPIKFAATLNQVDEDGGYTGLTQSEFVETVKMEAEA
                     IGYDGLIIVALDHGGPWLKDLHSIENWSLEETMRAVKKSLEASIDAGYDFLHIDPTVD
                     KTLLPGEIIPIERVVERTIELIKHAENYRRKKGIAPVGYEVGTEEVHGGLANLEVFDR
                     FLYLLKEGL"
     gene            complement(665..1633)
                     /locus_tag="PK016_08250"
     CDS             complement(665..1633)
                     /locus_tag="PK016_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HXL03084.1"
                     /translation="MRVGCLGELLVEIMRDKVDVPFDVPGVFVGPFPSGAPAIFADCL
                     ARLGEEVFFVGAVGEDDFGKMILRRLKEDGVDIGGVKILPDFTTGVAFVTYFRDGSRK
                     FIYHLSRAAAGQIFPSDIDEEQFSRLDYLHVMGSSMLINDNCRDAYLRALEIVKKKGG
                     KISFDPNFRPELLGASKARALFQPIVRESFVLFPTQEELEVLSGEKDIDKGTAVLLSQ
                     GPKIIALKQGKKGSTIFTPEGKFVIPAFPVEEVDPTGAGDCYCAGFLAGLSRGLPLIE
                     VGRLANAVGALAVTKKGPMEGAPTQKELEEFGKKFGKGEEGSAEKR"
     gene            complement(1848..2192)
                     /locus_tag="PK016_08255"
     CDS             complement(1848..2192)
                     /locus_tag="PK016_08255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXL03085.1"
                     /translation="MEEAELFHYWNKLLKEEGLGVYKGGREILLPDLTEKYPTPAREK
                     RKNISLPLEILSPRQQEVIYLLFYDRLTEEEAAEKLGISRSSLRTHRDRALEKLKKEL
                     EKDWWEDNNEVK"
     gene            2284..2685
                     /locus_tag="PK016_08260"
     CDS             2284..2685
                     /locus_tag="PK016_08260"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXL03086.1"
                     /translation="MFYNLFTMGDLQDKGQKLKELRKEKGWSVRKLAQRVGVSKSTVS
                     DVELGKRKPTANYLIKAAKALNIDERELLEWYGYLEPSPPGLDQKLERVVREFDTIFL
                     DHLGELSEESKKSLIDFLEYLIEKQKREGRD"
     gene            2697..3110
                     /locus_tag="PK016_08265"
     CDS             2697..3110
                     /locus_tag="PK016_08265"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HXL03087.1"
                     /translation="MVSLSPESIKRIESFSSSLPRINSETEALKLAEKEGIIVEFYDL
                     RGFKGVLAQRQGQTFLGVNKKLREAEEVKTILHELAHYFFHWNNRFRPIFLCHEYIIN
                     REEKEADFFAWCLMGEDWRETLSWKFLSENRTIRG"
BASE COUNT          862 a          729 c          607 g          951 t
ORIGIN      
        1 tagcccttct tttaaaaggt agagaaagcg gtcaaacact tccagattgg ccaatcctcc
       61 atgaacttcc tctgtcccta cttcataccc caccggagca attccttttt tgcggcgata
      121 gttttcggca tgttttatta gctctatggt tctctctact acccgctcta tgggaattat
      181 ttctccggga agaagagttt tgtctacggt ggggtcaatg tgcagaaaat catagcctgc
      241 atctatggat gcttccagag attttttaac tgccctcatc gtttcttcta aactccagtt
      301 ttctatactg tgtaggtctt taagccacgg tcctccgtgg tctagagcga caatgattaa
      361 tccatcgtag cctatagctt cggcttccat tttcaccgtt tccacaaatt cagattgagt
      421 aaggccggta tatcccccat cttcatccac ctgatttaag gtagcagcaa acttaatagg
      481 agcattggct cttttggctg aacgtaatgc ggctttgata accgtctccg aattaggaca
      541 agcggcaaaa aggctggaag gtttcccttc tatcttttct aactctttca ttttgtgcaa
      601 caggaaatcg actactggaa ctctttcttc ttctgccttg cggcggataa aactttggtc
      661 tactttatct cttctcagca ctaccttcct ctccttttcc aaattttttc ccaaattcct
      721 ccaactcttt ttgagtgggc gctccttcca taggaccttt tttagttaca gctaaagctc
      781 ccactgcgtt agctaacctc cccacctcta tcaaaggcaa ccctcgggag agaccggcta
      841 aaaatccagc acaataacaa tcaccagctc cggtggggtc tacttcttct accgggaaag
      901 cagggataac gaattttcct tccggggtaa aaatagtaga acctttcttt ccttgtttca
      961 gggcaattat tttaggtcct tgagataata ataccgctgt tcctttatct atatcttttt
     1021 ctccgcttaa cacctctaat tcttcctggg tgggaaataa aacaaaactt tcccgaacaa
     1081 tgggctgaaa cagagcccga gctttacttg cccccagaag ctcgggacgg aaattagggt
     1141 caaaactgat tttccctcct tttttcttta ctatttctag ggccctaaga taagcatcac
     1201 ggcaattatc gttaatgagc atactagagc ccataacatg gaggtaatcc agacgagaaa
     1261 attgttcttc atcgatatca gaaggaaata tctggcccgc agctgctcga gagagatgat
     1321 aaatgaattt tcggcttcca tctcggaaat aggtgacaaa agccaccccg gttgtgaaat
     1381 cagggagaat tttcactcct ccgatatcca ccccatcttc ttttaatctc cggagaatca
     1441 tttttccaaa gtcatcttct cctactgctc ctacaaagaa aacttcctct cctaggcgag
     1501 ccagacaatc agcaaagata gccggagcac cgcttgggaa aggccctaca aaaacaccag
     1561 gaacatcgaa agggacgtct actttgtctc tcattatctc taccaggagc tctcctaaac
     1621 aacctactcg cataactctc tctcctttca gggttttata actactttag ctctctattt
     1681 tttagagaaa aaacatagat aattattaaa gctggattgt cactcctttt tccataagca
     1741 ccagagaaat ctacgttact cgagaaacat gaatttcttt tgccacaatt ttttgactct
     1801 cctagaatag tagccagcga ggcaatcaat tcccttttta gtttattcta ttttacctca
     1861 ttattatctt cccaccaatc cttttccagc tcttttttaa gtttctctag agctctatca
     1921 cggtgcgtgc gaagagagga gcgagaaatt cccagtttct ccgctgcctc ctcttctgtt
     1981 agtctgtcat agaagagaag ataaattacc tcttgctggc gagggctgag aatctcaaga
     2041 ggaagagaaa tatttttcct cttttctctc gcaggtgtgg gatatttttc tgtgaggtca
     2101 ggtaaaagta tttctcttcc ccctttatat actcccagcc cttcctcctt tagtaacttg
     2161 ttccagtaat gaaatagctc tgcttcttcc ataaacgcct ccttatctgt ccctattata
     2221 ggacgaaacg tcctgtaagt caacaaataa ttttttgaaa ttcctctttc tttttagaga
     2281 ggaatgttct ataatttgtt tactatgggt gacttgcagg acaaagggca aaagttaaaa
     2341 gagctgagaa aagagaaagg ttggtcggtc aggaaattag cccagagagt gggggtttcc
     2401 aaatctactg tttctgacgt agagctgggt aaaagaaaac ccactgccaa ctatttgata
     2461 aaggcagcta aagctttaaa tatagatgag agagagttgc tggaatggta tggctacctg
     2521 gaaccctcac ctcccggttt agaccagaaa ttagaacgag tggttcgaga gtttgacacc
     2581 atctttttag accatctggg ggagttgagt gaggagtcca aaaaatctct catcgatttt
     2641 ctggaatatt taatagaaaa gcaaaaaaga gagggccggg attaaagatt taccccttgg
     2701 ttagcttatc tccagaaagt atcaaaagaa tagagagttt ttcttcctcc cttccccgga
     2761 taaatagcga aactgaagca cttaaattag cagaaaagga gggtataatt gttgagtttt
     2821 atgatttacg aggatttaaa ggagttctgg ctcaacgtca gggacaaact tttctgggag
     2881 tgaataaaaa actgcgagaa gcggaagaag tgaaaaccat tcttcacgaa ttagcacact
     2941 atttctttca ctggaataat cggttccgcc ccatatttct ctgccacgag tacatcataa
     3001 accgtgaaga aaaagaagct gatttctttg cctggtgtct tatgggggag gattggcggg
     3061 aaactctctc ctggaaattc ctgagcgaga atagaactat aagggggtaa ataccccctt
     3121 cataaccccc tttctttttc ttccctcgc
//
LOCUS       DAOSVE010000134         3113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_12708_length_3113_cov_67.234140, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000134 DAOSVE010000000
VERSION     DAOSVE010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12708_length_3113_cov_67.234140"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..863
                     /locus_tag="PK016_08270"
     CDS             99..863
                     /locus_tag="PK016_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HXL03088.1"
                     /translation="MKMKKYGVILLVLCVLMLGAVNGAVAEEKKTYIVGIDGDYYPFS
                     YIDENGNPAGFDVESIKWIAQEMGFEVEIVPIAWDGIIPSLLAKKIDMIYSGMTVTEE
                     RKEKVDFTDVYWVVNQAVATKEEANWTLEDFLAGKCSVGTQRGCSAAMWIEDNLVKSG
                     ILPEDKFKLYDNFPLAVTDLLNGRIETVIMDEPVIDKAIAGRPLKTIGVISTGEEYAI
                     AVRKEDTELKALLNEGLKRLMSSPYWEELIKKYEMK"
     gene            876..1553
                     /locus_tag="PK016_08275"
     CDS             876..1553
                     /locus_tag="PK016_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HXL03089.1"
                     /translation="MEGLSAVLESLPYILSGISVTLGLVALSLALGFALGVPMAVATV
                     YGNRIIVKIIRVFVFILRGLPNLVLLFLFYFGIFPLLGWNLSPFAIGTITLGLRSAAY
                     QTEIFRGAILSLEEGQMLAALSVGMTKNQAIWNIILPQALRLALPGWSNEYPILLTDS
                     SVTYAIGVAEILTRGSQMVTRTYQPMPIYLTCAIIFILLNWGGMQIIQWGEKKAYIPG
                     LGRKTET"
     gene            1553..2329
                     /locus_tag="PK016_08280"
     CDS             1553..2329
                     /locus_tag="PK016_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HXL03090.1"
                     /translation="MNTTDNTPILVVENIYKSYGKVEILKGASFTVNKGETKVIIGPS
                     GAGKSTLLKCINQLVIPDRGRIFLNGEEVLHSHSQINRFRQKIGMVFQNFYLFDHITA
                     LENVEIALLKVKKMKRSEARKKALEELARVGMEDKAHLYPAELSGGQAQRVSIARALA
                     MDPEVMLFDEPTSALDPELTGEVLEVMRNLAKEGMTMVVVTHEMGFASSVADEVLFME
                     EGKFLESGPPSQIFFHPREERTKKFLSRISELYGELNKNG"
     gene            2322..2978
                     /locus_tag="PK016_08285"
     CDS             2322..2978
                     /locus_tag="PK016_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HXL03091.1"
                     /translation="MDSLLFIRNELLPELGKGWIVTLKLVFLSIPFGLLTGILIAIGR
                     VYGNRLISSLCRLYVIFFKGCPLLLLLFIIYFGLPSLGITFSPFGASVLGFVICNGAY
                     TSEYIRGAIQSVKTGQMIAAQALGMTKWQAVFYIILPQAFRRALPGVGNEMIYLIKYS
                     SLAYMLTCIELTGAGKIVAARSFRFMETFTVVGIIYLFMVSIATKILQWAEKRLSYPG
                     "
BASE COUNT          920 a          589 c          679 g          925 t
ORIGIN      
        1 agcataatac caactctttt tcttcctaag agagttcact tatccaattg acagtaaata
       61 attcattgtt taatattaac aaaaatcctt aagggaggat gaaaatgaaa aagtacggag
      121 taattttatt agttttatgt gttttaatgt taggggcagt caacggagca gtggcagagg
      181 agaaaaaaac ttacatagtg ggaattgatg gcgattatta tccttttagc tatattgacg
      241 aaaatggcaa tccagcagga tttgatgtag agtccattaa atggattgct caagaaatgg
      301 gatttgaagt agagattgtt cctatagcct gggatgggat aattcccagt ttgctcgcca
      361 aaaaaatcga tatgatatat tcgggtatga cagttaccga agagcggaaa gagaaagtag
      421 attttactga cgtttattgg gtggtaaatc aggcagtagc cactaaagaa gaggctaact
      481 ggactctgga ggatttcctg gctggaaaat gcagcgtagg tacacaaaga gggtgttcag
      541 cagctatgtg gatagaagat aatttagtaa aatcgggaat ccttcctgaa gacaaattta
      601 aactttatga caattttcct ttagcagtaa ccgacctcct caatggccga atagaaacgg
      661 taatcatgga tgagccagta atagataaag ctatcgccgg aagaccgttg aaaacaattg
      721 gagttatttc tacaggagaa gaatatgcta tagcggtgag aaaagaagat actgaactca
      781 aagctctgct caatgaagga ttgaaaagat taatgagctc tccctactgg gaagaactga
      841 ttaaaaaata tgagatgaag tagaaaagga aaaaagtgga gggtctctct gctgttttag
      901 aatctcttcc ctacattcta tctggaatat cagtaacttt gggacttgtg gctctgtctt
      961 tggctttagg gtttgcctta ggagtgccaa tggcagtagc cacagtctat ggaaatcgaa
     1021 taatagtaaa aataatccgg gtattcgttt ttatattgag aggcttaccc aatttagtgc
     1081 ttctctttct tttttatttt ggtatctttc ctctcttagg gtggaacctc tctcctttcg
     1141 ctataggaac tataactctg ggtttgcgaa gcgctgctta ccagacagaa attttccgag
     1201 gcgccatcct ctctttagaa gaaggacaga tgttggctgc cctatctgtg ggtatgacca
     1261 aaaatcaggc catctggaat ataatattac cccaggctct acgcctggca cttcctggat
     1321 ggtctaatga atatcctatt cttcttactg actcttcggt aacttatgct attggagtag
     1381 ctgagatttt aacccgggga agccagatgg taactagaac ctatcagcct atgcctatct
     1441 atctaacctg tgctatcatt tttattctcc tcaattgggg aggaatgcaa attatccagt
     1501 ggggagaaaa gaaagcctat atccctggtt taggaaggaa aacagaaact tgatgaatac
     1561 tacagataac acacccattt tagtagtgga aaacatctac aaatcttatg gaaaagtgga
     1621 aatcctcaaa ggagcttcct ttaccgtaaa taaaggagaa actaaagtta taattggtcc
     1681 ttcaggtgca ggaaaaagca cccttcttaa atgtatcaat cagctcgtca tccccgatag
     1741 gggaaggata tttctcaacg gagaggaagt gctacattct cattctcaaa ttaatcgttt
     1801 tcgtcagaaa ataggtatgg tcttccagaa tttttatcta tttgaccata tcactgcttt
     1861 agaaaatgta gaaattgctc ttctcaaagt caaaaagatg aaacggtctg aggcacgaaa
     1921 aaaagctctg gaagagttag cccgggtggg aatggaagac aaagctcatc tttatcctgc
     1981 cgagctctcc ggaggacagg cacagagagt ttccattgcc cgagctttag ctatggaccc
     2041 tgaagtgatg ttattcgatg agcccacctc cgctttagat ccggaattaa ccggagaggt
     2101 gctggaggtt atgagaaact tagctaaaga ggggatgacc atggtggtgg ttacccatga
     2161 gatgggtttt gcttcctcag ttgctgatga agttctattt atggaggaag ggaaattttt
     2221 agaaagtggc cccccttccc agattttttt ccatcctcga gaagagcgaa ctaaaaaatt
     2281 cctcagccgg atttctgagc tatacggaga gttaaataaa aatggatagt ttgcttttta
     2341 tccgcaacga gctactcccc gagttaggga aaggatggat agtcacttta aaattagttt
     2401 ttctttctat cccttttgga ttactaaccg ggatattgat tgccatagga agagtatatg
     2461 gcaatcgcct gatttcttct ctctgtcggc tttatgtcat cttttttaaa ggttgtcctc
     2521 tccttctttt gctatttata atttattttg gactcccttc cttaggaatt actttttcac
     2581 cttttggggc ttcagtttta gggtttgtca tctgtaacgg agcctatacc tctgagtata
     2641 taagaggagc aatccaatcg gtaaaaaccg gtcagatgat tgctgcccag gctttaggaa
     2701 tgaccaagtg gcaggcagta ttttacatca ttcttcctca agcctttcgc cgggcactcc
     2761 ctggtgtggg aaacgaaatg atttacctca ttaaatactc ttccttagcc tatatgctta
     2821 cctgtataga gctaaccgga gcagggaaaa tcgtagccgc tcgaagtttc cgttttatgg
     2881 aaactttcac cgtggtgggg ataatttacc tattcatggt ctccatagcc actaaaattc
     2941 tccagtgggc agaaaagagg ctctcctacc ccggctaaag gaaataaact cacctaccct
     3001 ctttttgaga tttagctttt ttaatccagc gagcatattc tgctattgcc tcttccatag
     3061 gataacgagg agaaaagcct aactcctttt tagctaaaga aatatccaga gtt
//
LOCUS       DAOSVE010000135         3111 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_12725_length_3111_cov_68.269961, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000135 DAOSVE010000000
VERSION     DAOSVE010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12725_length_3111_cov_68.269961"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            633..2537
                     /locus_tag="PK016_08290"
     CDS             633..2537
                     /locus_tag="PK016_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL03092.1"
                     /translation="MKRSSLILFVSLLVLVVGFAGISFGQLSEDIPREETLIADVLSG
                     RVGTPGNFNVFTTAWRNPDRGMQQLVLEPLWIVDPTTGEVINALAKEGPIYNEDFTQM
                     TVKLREGCQWSDGVPITADDIVYGIELCMQNEGMGYNSQLSLNVNKVYKTDDYTVVFE
                     LKQPNARFHANLIDRWGGWRPFPKHIFEKVEDPLSFEFNPPISSGPYVLKDYDQAGYW
                     TLYERREDWDKTPTGMLYGMPKPKYVLFYYYGEPSNKVIAQANHNLDMTDLTMEAFRA
                     ALEQNKYSRGYRREYPWVVNTDPCMTGLMLNNDVSPFDLKDVRWALTLSIDIVDYIGI
                     AFDGAATMGVLHIPPTLAYQKWYYEPMEEWLKDFTLDIEINGEPFKPYDPDAPFRLVE
                     YAKSRGYTVPEDPEEIKEVFGRGWWKYAPDVAEQLLLRNGFTRDVDGKWLLPDGTPWK
                     IEILTPANMASPNYRNALAATQQWRNFGIEVTIVPSEAEQTLHGLGNYIVGAKWPSPE
                     PWGGHPDLYRVFSAWHSKYYKPVGEQASSTDGPASRWTDPRMDKVIEEMEKVDWNDPK
                     NTELGMEGLKIFIEEMPTIPTFSYPGVVAWDEYYWTNYPGAENPYTQPYHHWPNFKYM
                     LPFLESTGNK"
     gene            2917..3108
                     /locus_tag="PK016_08295"
     CDS             2917..3108
                     /locus_tag="PK016_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL03093.1"
                     /translation="MQQLLEEEVTDLPDGPLRESKRREENKSRYRGYEPRKIKTKDKR
                     YISEVRDIEEPFLLGWELS"
BASE COUNT         1051 a          500 c          695 g          865 t
ORIGIN      
        1 ggggttgata aggggcagag cccccttagt cattataata aattttgcga ttaactatat
       61 atactgaata atctaaagag gaataacttt caaaaccgtt ttaattagta aattttcttt
      121 ttatttactg ttgacaatta ttttaaaaat aatctatact ggtagcggtg acctgtgtcg
      181 atttgatttg ctaaaacgat ttagccacga agaagtatgc actataattg tttcgagtgg
      241 aaaaggtaag gtatcagagg aaaactaaaa aggtgtgaaa aacagtacga aagttggggg
      301 gtatattacc tgatattggg actcaaggtt tagcaaggga taaattgatg ctattttgct
      361 ttttatagag gagtggttaa gtgttttttg attttcattc ctgtaaagta atattgcctt
      421 cagatagcag caaggagaga gtttttagcg aacattctct ggataaattg cttacaacca
      481 tttaagaaaa ctgaacgtgc tttaattgtg aaggatgtat cccggatata tccgggatga
      541 tctctaagca agaagatggg aggtgatgag aaaaataaaa gcttagagaa tttaagagta
      601 aaaatactgt attcaaactg gaggtgtggt tagtgaaaag aagctcttta atcttgtttg
      661 tatctttgtt agtgctggtg gtgggttttg ccgggataag ctttggtcag ctatctgaag
      721 atattcccag agaagaaacc ctgattgctg atgtattatc aggcagagta ggaacaccgg
      781 gaaactttaa tgttttcacc actgcctgga gaaacccgga tcgcggaatg caacaactgg
      841 tattagaacc tctctggata gttgacccta cgacaggaga agttataaac gctctggcta
      901 aagaagggcc tatttacaat gaagacttca ctcagatgac agtaaagctg agagaaggtt
      961 gccagtggag cgatggagtt cctataaccg cagatgacat agtatatgga atagagttat
     1021 gtatgcaaaa tgaaggaatg gggtacaact ctcagcttag ccttaatgta aataaagtat
     1081 ataaaactga tgactatacg gtagtatttg agcttaaaca acctaatgcc cgatttcatg
     1141 ctaacttaat agatagatgg ggtggctgga gacctttccc taaacatatt tttgaaaaag
     1201 tagaagaccc tctatccttt gaattcaatc ctcccataag tagcggtcct tatgtactca
     1261 aagattatga ccaggcaggt tactggacgt tatatgagag aagagaagat tgggataaaa
     1321 ctcctaccgg aatgttatat ggtatgccca aacccaaata tgtacttttc tactattatg
     1381 gagaaccaag taataaagtt atagctcaag caaatcataa cttagatatg accgatttaa
     1441 ctatggaggc attccgagca gctctagagc agaataaata ttcaagaggt tatagaagag
     1501 aatatccctg ggtagttaat accgacccct gcatgacggg attaatgtta aacaacgatg
     1561 tatccccatt tgacctaaaa gatgtcagat gggcgctaac tctctcgata gacatagtag
     1621 attacattgg aatagctttt gatggtgctg ctactatggg ggtattgcat attcctccaa
     1681 cacttgccta tcaaaaatgg tattatgaac ctatggaaga atggttgaaa gatttcactc
     1741 tggacataga aataaatggg gaacctttca aaccttatga ccctgatgcc cccttccgac
     1801 ttgttgagta tgcaaagagt agaggctaca ctgtacctga agaccccgaa gaaattaaag
     1861 aagtttttgg acggggatgg tggaaatatg ctcctgatgt agcagaacaa ttattactca
     1921 gaaatggatt taccagagat gtagatggga aatggcttct gcctgatgga acaccgtgga
     1981 aaattgagat attaactcca gcaaatatgg cctctcctaa ctacagaaat gctcttgctg
     2041 ctactcagca gtggagaaac tttgggatag aggtaacgat tgtgccatct gaagctgaac
     2101 aaaccttgca cggtttaggt aattacattg ttggtgcaaa atggccttcg cctgagccct
     2161 ggggaggaca tccggatttg tacagagttt ttagtgcctg gcattccaaa tattataaac
     2221 ctgttgggga gcaagcttct tcaacagatg gtcctgcttc taggtggaca gaccctcgga
     2281 tggataaagt aatagaagaa atggaaaaag ttgattggaa tgacccgaaa aatactgaac
     2341 tgggtatgga agggctaaag atatttattg aagagatgcc cactatcccc acatttagtt
     2401 atccaggagt ggtagcctgg gatgaatatt actggactaa ctatcctgga gctgaaaatc
     2461 cttacaccca accatatcat cactggccga actttaaata catgcttccc ttcctcgaat
     2521 caacaggaaa taaataaaaa cacttgtagt acccaggcct cttgggaggc ctgggtaatg
     2581 tgtgtttttt ttggtgtata tgacagttat aacccttagg aactttaaga tttgtaagcc
     2641 caactcaaat ggtgttcaga ttagaagatt aaaacaataa aaatagataa actcagctga
     2701 tgatatggag attcaacata gcttcttttt gagaataaca agtaaaatat gagctaatac
     2761 aagaaggatc cacttataaa aagaatacca aagagtgaag tacgagacag gagttgaatc
     2821 aaatattaga aggaattttg gatgatttag ctgaaggtaa attagaagaa agagaatcct
     2881 ggttgaggag gttataaaaa aagagctgct tttttcttgc aacaattatt agaggaagaa
     2941 gtaactgatt taccagatgg accattacga gagagcaaaa ggagagaaga aaataaatca
     3001 agatatcgag gctatgagcc cagaaagata aaaactaaag ataaaagata tatctcagaa
     3061 gtaagagata ttgaagaacc attcctatta ggctgggagc tttcttaaaa g
//
LOCUS       DAOSVE010000136         3049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_13041_length_3049_cov_64.330661, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000136 DAOSVE010000000
VERSION     DAOSVE010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13041_length_3049_cov_64.330661"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PK016_08300"
     CDS             <1..387
                     /locus_tag="PK016_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HXL03094.1"
                     /translation="EIWRRMFDAIPSDYFGLNLDPSHLIWQQIDYVKVIYDFREKIFH
                     VHAKDTRIDWNKLNEVGIFGFGWYIDKVAGTGDIDWNSFIATLYEVGYDYVVSIEHED
                     RSFEKDQESKLRGILLAKQLLEPYIV"
     gene            421..1524
                     /locus_tag="PK016_08305"
     CDS             421..1524
                     /locus_tag="PK016_08305"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HXL03095.1"
                     /translation="MGRRTVEVRRIGVLSGGGDCPGINAVIHAVVKRAILRYGWEVIG
                     VLDGFEGMVEGKWRKLEYNDVSGIMPLGGTILGTSNIADPFHYPVREGEKLVFRDFSQ
                     KVVDNYHQMDIQALITIGGDGTFNVSQKFVERGLNVVGVPKTIDNDLGGTDLTFGFDS
                     AVAVVTDAVDRLHTTAQSHHRVMVVEVMGRYAGWIALYGGIAGGGDIILIPEIPFDMD
                     KICEFILKRRESGKRFSIVIVAEGAHPKGGEMVVQKIVEESHDQIRLGGIGYEVGQEI
                     ERKTGLETRTVVLGHLQRGGSPTAFDRILATRFGAKAVDLVAEGKYGYFPALQGEEIV
                     PLPMTAAICNLKVVPLESSLLDVARSLGTYMGD"
     gene            1528..2304
                     /locus_tag="PK016_08310"
     CDS             1528..2304
                     /locus_tag="PK016_08310"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HXL03096.1"
                     /translation="MTARLSVALDVAKKAGNFAMAHQNSIREIKEKNSPVDVVTEVDK
                     EVQEMIIKELSSHFAQDLFWGEESGYPLEDFSSTWVIDPIDGTSNYIHNLPLYGISIA
                     YLREGEPIIGVLEIPRLRETFWAEKGGGAFLNGERIRVSEIKEMHRAIVGTGFPHRGA
                     KWQIMEPVYAQILSECQALRSLGSAALGVAYVACGRLEGYLQLGLSFYDIAAAICLVR
                     EAGGKIRELESEKWNVKSRSILATNSKIEMGKFLKDFSLP"
     gene            complement(2534..2728)
                     /locus_tag="PK016_08315"
     CDS             complement(2534..2728)
                     /locus_tag="PK016_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015791114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HXL03097.1"
                     /translation="MPNITVEGPSIPDIEKKRKLVKTITQVATEVYGLPAQAIVVTIR
                     ENSPENVAIGGNLLSDQKKS"
BASE COUNT          856 a          514 c          757 g          922 t
ORIGIN      
        1 gaaatctgga ggagaatgtt tgatgccatt ccctctgatt atttcggcct caatcttgac
       61 ccttctcatc tcatctggca gcagattgat tatgtaaagg taatttatga ttttagagaa
      121 aaaatcttcc atgttcatgc taaagatacc cgtattgatt ggaataaatt gaatgaggtg
      181 ggaatctttg gttttggctg gtatatagat aaagtagctg ggacaggaga tatcgattgg
      241 aattctttca ttgccactct ttatgaagtg ggttatgatt atgtagtgag tattgaacac
      301 gaagatagaa gctttgagaa agaccaagag agcaaattgc gaggtatttt actggctaag
      361 caactgttag aaccatatat agtataatta atccaaattt ggtaaatacc tcagtttgca
      421 atggggagga gaacagtgga agttagaaga ataggagttt taagcggagg aggggattgt
      481 cccggtatca atgcggttat tcatgccgtg gtaaaaagag ctatccttcg ctatggctgg
      541 gaagtcatag gtgtgttaga tggttttgag ggaatggtgg aagggaaatg gagaaagtta
      601 gaatacaatg atgtttcagg aataatgcct ctggggggaa ctatcttagg gacttccaat
      661 atagcagacc ccttccatta tccggtgcgc gaaggagaaa agttggtttt ccgtgacttt
      721 tctcaaaaag tggttgataa ttaccaccaa atggacattc aagccctgat tacgatcggc
      781 ggcgatggca cttttaatgt ttctcaaaag tttgtagaaa gaggtttgaa tgtagtagga
      841 gtgcctaaaa ccattgataa tgatttggga ggaactgatt taacttttgg ctttgattca
      901 gcagtggcgg tggttactga tgctgtagac cgtttacaca ctactgctca atctcaccac
      961 cgggttatgg tggtggaggt gatgggaaga tatgcagggt ggattgctct ttacggagga
     1021 atagcaggag gaggagatat cattctcatc cccgagattc cttttgatat ggacaaaatt
     1081 tgtgaattca ttctgaaaag aagagaatcc ggaaagagat ttagtattgt aatagtggca
     1141 gagggagctc atcctaaagg gggagaaatg gtggtacaaa agatagtaga agaaagccac
     1201 gaccagattc gtttgggagg catcggctat gaagtggggc aagaaataga gagaaaaacc
     1261 ggcctggaaa caagaacagt ggtgctggga catttgcaaa gaggaggttc tcctacagct
     1321 tttgaccgaa ttttagctac tcgctttgga gctaaggctg tagatttagt ggcagaggga
     1381 aaatatggtt attttccggc tcttcaagga gaagaaattg ttcccttacc catgacagca
     1441 gctatttgta acttaaaagt tgttcccctc gagtcatctc tgttagatgt agctcgttct
     1501 ttagggactt atatgggaga ttaaaaagtg actgctcgtc tttcagtggc tttagatgtg
     1561 gctaaaaaag caggaaattt tgcaatggct catcagaact ctattcggga aataaaggaa
     1621 aaaaatagcc ctgttgatgt ggttactgag gtagataaag aagttcagga gatgataatt
     1681 aaagaactat cttctcactt tgctcaagac cttttctggg gagaggaaag cggttatcct
     1741 ctggaagatt tttcttctac ctgggtaatt gaccccattg acggcaccag taactacatt
     1801 cataacttac ctctgtatgg tatttctatt gcctatctta gggaaggaga accaataata
     1861 ggggttttag aaattccccg tctgagagaa actttctggg cagaaaaggg agggggagct
     1921 tttctgaacg gagaaagaat tcgagtttca gagataaaag agatgcatcg tgccattgtg
     1981 ggaacaggtt tcccccaccg gggtgccaag tggcaaatta tggagcctgt ttatgcccag
     2041 attctttccg agtgccaggc tctgcgctct ttaggaagcg ctgctttggg agtggcttat
     2101 gtggcttgtg gccgtctgga gggatatcta cagttgggat tatcttttta tgatatagca
     2161 gcggcgatat gcttggtgcg agaggcaggg ggaaaaatca gggagttaga aagtgagaaa
     2221 tggaacgtta agagccgttc tattctggcc actaactcta aaatagagat gggaaaattt
     2281 ctaaaagatt tctcccttcc ttaagcggtt tgggggctta aaatagcggg gagtattttt
     2341 aatctttcag gagtaagata tttaaagaat aaccgggtac aattacccgg ttattctaac
     2401 ctaaaacagc tataaaagag ttataatatt ttatcttagt ggattttctc tcagatatgt
     2461 agtatagact tttctatcta aaatacctta aaaccactaa ccaagggctt tttccgatat
     2521 cgggttttca gttttagctc tttttctggt ccgataataa gtttcctcca attgctacgt
     2581 tttcgggaga gttttcccgg atagttacta caatagcttg agctggcaat ccgtatactt
     2641 ccgtagccac ttgagttata gttttcacca gttttctctt tttttcaata tcgggaattg
     2701 atggtccttc aacagtaata ttaggcataa ttctcacctc tctggacatg ttttttaagc
     2761 taaaatactt gaccctatag gtcattataa tgtttcttcc ttttctcttc aatccttgtg
     2821 agctggctat cctttttcct cagtggtctt tgtttcaggc ttttgcgttt ttatggaggt
     2881 tgtaaggagg ccttttcccc tcatagttgg gattcgtcct caccctctct cggagggtaa
     2941 gggaaaaaaa tatgtggggc tctccctggg agggtgaggg aaaaatacat agagggtttc
     3001 ccgtcctatg tttctaccaa cccaggatgt ccaaaagcta ccgagattg
//
LOCUS       DAOSVE010000137         3039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_13099_length_3039_cov_64.753351, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000137 DAOSVE010000000
VERSION     DAOSVE010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13099_length_3039_cov_64.753351"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..801
                     /locus_tag="PK016_08320"
     CDS             166..801
                     /locus_tag="PK016_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HXL03098.1"
                     /translation="MDESWKLLSIFGSAFLVGFSGALMPGPLLALVLSGTYRVGYKAG
                     PLVVLGHGILELGLVVALMLGLGKVLNIPLVQQVIGVGGGIVLAYLGIDMLRAVRNSE
                     TLSVKKGSSWEGNLVLKGLLVSLSNPYWIMWWATVGLALLLSAISFPWWGVVFFFLGH
                     IFSDFVWYSAVSFTLDRGKNFLSPRFYQWLVGICGGFMLFFGIYFISGVIQ"
     gene            802..1104
                     /locus_tag="PK016_08325"
     CDS             802..1104
                     /locus_tag="PK016_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL03099.1"
                     /translation="MIKLVLEPVPYRYEETVYFYWEYELNSPYQLCFIPFGSLNKFLE
                     ETVRRETEIPESIHIILEDGRIKIWLPVTFYSEQLFDKVENILREKIRTLLEDVMF"
     gene            complement(1101..1856)
                     /locus_tag="PK016_08330"
     CDS             complement(1101..1856)
                     /locus_tag="PK016_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HXL03100.1"
                     /translation="MTGEAIILGLIQGLTEFFPISSSAHLILLRPIFNLGEQSLWFDV
                     LLHGGTWLAVLIYLIPYYSRAFRQPRIILNILLATIPAGLLGYLFEDIIENYLRSRFL
                     EICLLLIILGVIFLLVKERGDKTLENMTGKEALLIGVAQALALFPGVSRSGITLLAGF
                     LCGLQKKEAVLFSFFLSATTIGGAFILGIRSLSVSGNLFSPELVGGFLSSFGSGLLAC
                     FLLLRIVKEKGLKPFGYYRIIFGGIMFIWFLLH"
     gene            1798..2928
                     /locus_tag="PK016_08335"
     CDS             1798..2928
                     /locus_tag="PK016_08335"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HXL03101.1"
                     /translation="MGKNSVNPWIKPNMIASPVIMFPFLFACYSIIELKGEQYQEKKK
                     KMERILPATDENLKKGAEILRKGGLVAFPTETVYGLGANVFLPQAVARIFEVKRRPHF
                     DPLIVHISHHDMVSRLFREIPPLAEVLMEKFWPGPLTLVLPKKEEVPDIVTAGLSTVA
                     VRMPAHPVALKLIEWADTPVAAPSANRFGHLSPTQAEEVALDLEEGVDLILDGGKTQI
                     GVESTVIMIEEGRIFLLRPGGASVEEIERWVGKVETPPPSDKILSPGALKKHYAPQIP
                     LYLFEGKIEELFSCDLTGVVIVSPFPITDSRGEIRVLSEEGDIKEVASNLFTVLRELE
                     RGPFRAIISLTVEEKGLGRAIMDRLKRASSGYIKVKDGRLSL"
BASE COUNT          880 a          556 c          687 g          916 t
ORIGIN      
        1 gcaaaagaga tgaaaaggac taaaactacg gaggaagaaa aagccttcct ccgggcgtgg
       61 cgatcttgag tttttgactt ccggtataat ttacttttta aagtttctaa cattttaacc
      121 agttttgcgg gatactataa ttgttatata ataaataaaa aagaaatgga cgaaagctgg
      181 aaattgttat ccatctttgg tagtgctttc ctggttggtt tttcaggagc gttgatgcca
      241 ggtcctctct tagctctggt cctttccggg acttatcggg taggttataa agctgggcct
      301 ctggtagttc tggggcatgg gattttagag ctgggtttag ttgtagcttt aatgctcggc
      361 ctggggaaag tgctgaatat acctttagtt caacaggtta taggggtggg gggaggaata
      421 gttttagcgt atctggggat agatatgttg agggcagtgc gtaactcaga aactttatct
      481 gtaaagaagg gctcaagctg ggaaggcaac ctggttttaa aaggcttgct ggtcagtctt
      541 tctaatcctt actggataat gtggtgggct acggtggggt tagctttgct tctttctgct
      601 atttcttttc cctggtgggg ggtagtgttt ttcttcctcg gccacatttt ctctgacttt
      661 gtctggtata gtgcagtttc ttttacttta gaccggggta aaaacttcct ctctccccgt
      721 ttttaccaat ggttagtggg aatttgtgga ggatttatgc ttttctttgg tatttacttt
      781 atatcagggg tgatccaata aatgattaag ttagttttag agccggtgcc ttaccgatac
      841 gaagaaactg tttactttta ttgggaatat gagctaaata gtccttatca gctctgtttt
      901 ataccctttg ggagtcttaa taagttttta gaggagacgg tgaggagaga aactgaaatt
      961 cctgaaagta tccatattat tttagaggat ggaaggataa aaatatggtt gccggttacc
     1021 ttttattctg aacagttatt tgataaagta gaaaacattt tgagagagaa aattcgtact
     1081 cttctcgaag acgttatgtt ttaatgaagc aaaaaccaga taaacattat tcctccaaaa
     1141 atgatgcgat agtaaccaaa aggttttaac cctttttctt tcacaattct cagtaataaa
     1201 aagcaagcca gtaatccact accaaaggaa gataaaaaac caccaacgag ctccggtgag
     1261 aagaggtttc ccgaaacact caggcttctg attcctaaaa taaatgctcc tccaatggtg
     1321 gtggccgata aaaagaaaga aaaaagcact gcttcttttt tttgcaatcc gcagagaaat
     1381 ccagctaaaa gggtgatacc tgagcgagaa actcccggaa aaagagccag ggcttgagct
     1441 accccgatta agagagcttc cttcccggtc atattctcta aagttttatc tcctctttct
     1501 tttaccagta aaaatatcac ccctaaaata attaacagca ggcatatttc taaaaacctg
     1561 cttctcagat aattttctat tatatcttca aatagatatc ccagtaatcc tgcaggaatg
     1621 gtggctaaaa ggatgttcaa aataattcgg ggttggcgaa aagcccggga ataatagggt
     1681 ataaggtaaa tcaacactgc aagccaggtc cccccatgta gtaatacatc gaaccacaag
     1741 ctttgttccc ctaagttaaa tatcggacgg agaagaataa gatgagcaga agaggaaatg
     1801 ggaaaaaact cagttaaccc ctggattaaa cctaatatga ttgcctctcc ggtcataatg
     1861 tttcctttct tatttgcttg ctattctata atagaactaa aaggagagca atatcaggag
     1921 aaaaagaaaa aaatggagcg aattcttccg gctacagatg aaaatcttaa aaaaggagca
     1981 gagattttaa gaaagggagg gctagtagct tttcctactg aaactgtcta tgggttggga
     2041 gctaatgtat ttttgcctca agcagtggct cgtattttcg aagttaaaag aagacctcat
     2101 tttgaccctc ttattgtaca tatttctcac catgacatgg tctcccgtct ctttcgagaa
     2161 attccccctc ttgcagaggt gctgatggaa aagttttggc ctggtccttt aactttagtt
     2221 ttacccaaaa aagaggaggt tccggatatt gttactgctg gtctttctac tgtggctgtt
     2281 cgtatgccag ctcatcctgt agctttaaaa ttaatagagt gggcagacac ccccgttgct
     2341 gctcctagcg ccaatcgttt cgggcatctg agccctactc aagcagagga agtagctctt
     2401 gatttagaag aaggggtaga tttaatcctg gatgggggta aaacacaaat tggagtagaa
     2461 tcaacagtaa ttatgataga agaaggaagg attttccttc ttcggccagg aggagcgagc
     2521 gttgaagaaa tcgaaaggtg ggtggggaaa gtcgagacac cgcctccttc cgataaaatt
     2581 ctttctcctg gtgctttaaa gaaacactat gctccgcaga ttcctcttta tctttttgaa
     2641 gggaagatag aagaactctt ctcctgtgac cttacaggag tagttattgt ttctcctttc
     2701 ccgattactg attcccgagg ggagataagg gttttgagtg aagaggggga tataaaggaa
     2761 gtagccagta atctattcac tgttttgcga gagttagaga gaggaccttt tcgggccata
     2821 atcagcttaa ctgtagaaga aaaaggactg ggaagagcaa ttatggatcg gctaaagaga
     2881 gcttcttctg gatatatcaa ggtgaaagat gggagattaa gcttataaat tcctttgctt
     2941 cttctcctac ttctttttcc ttccttctcc tcctctccct tgaggctgat ccaagcacac
     3001 acttaacacc gaggctaaga tagcgtagat tatagttaa
//
LOCUS       DAOSVE010000138         2995 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_13348_length_2995_cov_96.792517, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000138 DAOSVE010000000
VERSION     DAOSVE010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13348_length_2995_cov_96.792517"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..1588
                     /locus_tag="PK016_08340"
     CDS             128..1588
                     /locus_tag="PK016_08340"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HXL03102.1"
                     /translation="MTDKDKFLGPDWLLDTKEGVKLYHDVAVPLRHRLGIVDVHTHYS
                     LRQIIENQPFPNIWKAEVLEDREEYANCDHYIIQLASKFPGFSQALARDPHLSDYAKW
                     MALSKVFPDLEGNHVHQWLHLDLKRIFGIEELLSADTGDEIWRLTEECFKKEDMFPRA
                     LLKRMGARVIFTTDDPVDDLSYHKIAKETINDINFLPCFRPDAYCNIFDEKWRDNVEK
                     ICQLTEEDTSLQGLVEALRKRHDYFAEMGAKASDHGLLEPYGLEVSEERAEQIFRRAY
                     NQKEKFTIRSSETREFISYLMHKFCEMNQDKKMVTQIHYGALRDANQYLYENWGANVG
                     GDIATEDITIVEFLKPLLSKFFSGKSDNESHLVLYPMNQAFSHINLTLERAFPNVHQG
                     FPWWHNDAPYVMEHFLLHTANSSLLSSVAGPVSDARKILSEGSRFEVFDRVICKAVGR
                     LLSEGQISYQGAINAVKGLMYGNQIRIFDLEGHGEI"
     gene            1633..2667
                     /locus_tag="PK016_08345"
     CDS             1633..2667
                     /locus_tag="PK016_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXL03103.1"
                     /translation="MDEARWSCFVDAVSGRNKKVVVALIVDSPWIPGFCGMSHFDYYL
                     FPERWLEANIKVYSSYPDIIFLPGFWVEYGMATEPSAFGCKITWWDNNTPSITPMLSS
                     IDEINSIRVPQPREDGLMPFVLHWYKWAQDILQEHNMSIKMVAARGPLALAAHLRGMT
                     DFLMDLKLEPDNTKKLLEITTTTVIEWLKAQVEVLGTVEGVMVLDDIVGFLSRSDYME
                     FAHPYLKAIFDTFPSMIKVYHNDAHITPFVDQLPETGLDVLNFSHELDIVEVAQKVGD
                     KICLMGNIPPLDVLVRGDISEVKSAATRCLDSVSKNHIRRFILSAGGGVSPDTPGENV
                     DVLNQLANSL"
BASE COUNT          886 a          525 c          655 g          929 t
ORIGIN      
        1 atttgataga gagaagcaag gcttcggaaa ggagaagaac taaagtatcc tctttacagc
       61 aaaaagggac ttgaacataa aattatgaca agcatcctaa aaaattcttt tgaaaggaga
      121 attaactatg actgataaag ataagtttct aggacccgat tggctactgg atactaaaga
      181 aggagtaaaa ttgtaccatg atgttgctgt tcccttaaga cacagattag gaattgttga
      241 tgttcatact cattatagtt taagacaaat tattgaaaat caaccattcc ccaacatctg
      301 gaaagctgag gttttagaag acagagaaga atatgctaat tgcgaccatt atattatcca
      361 gctggcctca aaattccctg gtttttccca ggctctggca agagatcctc atctttctga
      421 ctatgccaaa tggatggcct tgagtaaagt ttttcctgat ctggaaggta atcatgttca
      481 tcagtggctt catctggatc taaaaaggat attcgggatt gaagaacttt tgagtgctga
      541 tacgggagat gaaatctgga gattaaccga ggagtgtttt aaaaaagaag acatgtttcc
      601 ccgggcattg cttaaaagaa tgggagccag ggttattttt actactgatg acccggtgga
      661 tgacctctct tatcataaaa tagctaaaga aaccataaat gatataaatt ttctcccttg
      721 cttcaggccc gatgcttatt gtaacatttt tgacgagaag tggagagata atgtagaaaa
      781 aatatgccag ctgacggaag aagatacctc gcttcagggt ttggttgagg cattgcgaaa
      841 gcgtcatgat tactttgctg agatgggagc taaggccagt gaccatggcc ttcttgaacc
      901 ttatggactg gaagtgagtg aagaaagagc ggagcaaatt tttagaagag cctataacca
      961 gaaggaaaag tttaccatca ggtcttctga aactagggag tttatttctt atttaatgca
     1021 taaattttgt gaaatgaacc aggataaaaa gatggtaacc cagattcact atggtgccct
     1081 acgagatgca aaccaatatc tctatgaaaa ttggggagct aacgtaggag gtgatatagc
     1141 taccgaagat attactattg ttgaattcct aaaacctctt ctttcaaaat ttttcagtgg
     1201 aaaaagcgat aacgagtccc atctcgtact gtatcccatg aatcaggcct tttctcatat
     1261 taacctcaca ctggaaagag ctttccctaa tgttcaccaa ggcttccctt ggtggcataa
     1321 tgatgctccg tacgtgatgg aacattttct cctgcatacc gctaactcat ctcttttaag
     1381 ttcagtagca gggccggtga gtgatgcaag aaagattttg tctgaaggaa gtagatttga
     1441 ggtctttgat agagtgattt gtaaggctgt aggaagattg ctctctgagg gtcaaatctc
     1501 ttatcaggga gctataaacg cggtgaaagg cttgatgtat ggaaatcaaa tccggatatt
     1561 tgatctcgaa ggtcatggtg aaatttagta ttttaccatc agtggttaaa tcacataaaa
     1621 ggggtgaaac tcatggatga agctcgatgg agttgctttg tagatgcggt atctggtaga
     1681 aataaaaaag ttgttgtagc tctgattgtt gatagcccct ggattccagg tttttgtggt
     1741 atgtcccatt ttgattatta tttatttcct gaacgttggc tggaggctaa tatcaaggta
     1801 tattcttctt accctgacat tattttcctt cctggtttct gggtagaata tgggatggct
     1861 actgaaccat ctgcttttgg ttgtaaaatt acctggtggg ataataatac tccttccata
     1921 actccaatgc tatccagtat agatgagata aatagtatcc gagttcctca acccagggaa
     1981 gatggtttga tgccctttgt tctccactgg tataagtggg ctcaagatat actccaagag
     2041 cacaatatgt ccataaaaat ggttgctgct cgtggtcctc ttgctcttgc agctcattta
     2101 agaggaatga ccgattttct gatggactta aaactggaac ctgataacac caagaaacta
     2161 ctggagataa ctactaccac agttattgag tggcttaaag ctcaagtaga ggttttagga
     2221 actgtagagg gagtgatggt acttgatgat atagtagggt ttttatctag aagtgattat
     2281 atggagtttg cccatcctta tctgaaggca atttttgata cttttcccag catgataaag
     2341 gtatatcata atgatgccca tattactcct tttgtagacc agctccctga aactgggtta
     2401 gatgttttga attttagtca tgagttagat atagtggaag tagcacaaaa agtaggagat
     2461 aaaatatgtc ttatgggaaa tatacctcca ctggatgtcc tagtgagagg tgatatttct
     2521 gaagttaagt cagcagcaac tcgttgcctg gatagtgtct ctaaaaatca cattcggaga
     2581 tttatcctct ctgctggagg tggagtatct cctgacacgc ctggtgaaaa tgtggatgtc
     2641 ttgaaccaac tggctaacag tttatagtat cccgcaaaac tgattaaaat gttaaaaact
     2701 ttaaaaagta aattatatcg aaagtcaaaa actcaaggtc gctacgcata gagaaagact
     2761 ttgtctttct ctatggcctt tatttctttt gttttttaag ttttcttaat cttgcgtcgc
     2821 agactgcctt tttctccagc atactggtgg atattttccc accagcagtt tttgtttatt
     2881 tgctttttaa gtctttgttc ttttcttttg cttttggggg gttataaggg ggtgaaaccc
     2941 tcttataacc ttcaccctca ccctaaccct ctcccttcga gggcgaggga aggaa
//
LOCUS       DAOSVE010000139         2932 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_13684_length_2932_cov_73.049705, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000139 DAOSVE010000000
VERSION     DAOSVE010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13684_length_2932_cov_73.049705"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      72..168
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            255..1538
                     /gene="thiC"
                     /locus_tag="PK016_08350"
     CDS             255..1538
                     /gene="thiC"
                     /locus_tag="PK016_08350"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HXL03104.1"
                     /translation="MLKTQLDFARAGIVTEEMRRAAEDEAISPEELSHLIAEGVAVLP
                     KNVNHSFPRIKAIGSKLKTKVNANLGTSDECADLEFEKRKLSAAIKAQADSIMDLSTG
                     GDLHLWRCFFLEESPVMVGAVPVYAVAADMARRGEPLEKMTEEMLFTSIEEQCRQGVD
                     YITVHCGVTRESSGRLNTFKRIMPCVSRGGSIHLYWMRKTGKENPLYEHFDDLLEIAH
                     HYDVTLSLGDGLRPGTILDAIDGPEIEELMILAELSKRARARGVQVMIEGPGHVPFTH
                     IQSHVQLEKRLCEGAPFYVLGPIPTDIAPGYDHITSAIGGALAASAGADFLCYVTPAE
                     HLSLPDENDVYLGVMAARVAAHVGDIAKGVPGAIEKDKRISLAREHLDWEGMMAEALD
                     PELVRCKFNLASGRQSCTMCGKLCAVKMTRGAYEL"
     gene            1553..2335
                     /locus_tag="PK016_08355"
     CDS             1553..2335
                     /locus_tag="PK016_08355"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HXL03105.1"
                     /translation="MKLDEVSITKAIVERYFERLMNNLEIDVAIVGGGPSGLVAGYFL
                     AKAGCRVVLYERKLSVGGGMWGGGMLFNEIVVQQDARRLLDELEVRTTPYKEPGYYTA
                     SSVESVSTITSKAVKAGLSIFNCVSVEDVVVKNDRISGLVINWTAVSMAGLHVDPLSV
                     RSRFIIDATGHDTEVVAMIERKAPGKLATPSGNKEGEKFMNPEEAEKLTLENTREVFS
                     GLYVTGMACNATFGGPRMGPIFGGMLLSGEKVAQLILKEFSR"
     gene            2404..2491
                     /locus_tag="PK016_08360"
     tRNA            2404..2491
                     /locus_tag="PK016_08360"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2439..2441,aa:Leu,seq:gag)
     gene            2539..2612
                     /locus_tag="PK016_08365"
     tRNA            2539..2612
                     /locus_tag="PK016_08365"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2571..2573,aa:Gln,seq:ctg)
BASE COUNT          809 a          527 c          777 g          819 t
ORIGIN      
        1 ctttaagatt ccagttttcg cgtgattgac gtaaaaaaga aacggtgata gtattattaa
       61 aggtaggttg tatatcgcca tgggggcgct cttaaagctt gggctgagaa ggtaccccgt
      121 gaacctgatc caggtaatgc tggcggagga aatggcggga aggaagttaa aagtatagct
      181 ctggtttaat ttttaagctg ctttttttct tcttcagttg ctttttcctc ttctaaatta
      241 tacaggagga aaatatgttg aaaacccaac tcgattttgc cagggcaggc atcgtaactg
      301 aagagatgcg tcgtgccgct gaagatgagg caataagtcc ggaagaactc tcacacctta
      361 tagcagaagg agtagcagtt ctacctaaaa acgtaaatca ctctttccct cgcataaagg
      421 ctattggtag taagctaaaa accaaggtga atgccaatct ggggacgagt gatgagtgtg
      481 ctgaccttga gtttgaaaaa cgcaaacttt ctgcggctat aaaagctcaa gctgattcca
      541 ttatggatct ttctaccggg ggagacctcc atctttggag atgtttcttt ttagaagaat
      601 caccggttat ggtaggggct gtacccgtat atgctgtcgc agcagatatg gccagaagag
      661 gtgaacctct ggagaaaatg actgaagaaa tgctttttac atcaatagaa gagcaatgtc
      721 gccagggtgt agattatatt accgttcact gtggcgtaac acgtgagtct tctggtaggc
      781 tcaacacttt taagcggatt atgccttgtg tgagcagagg cggctcaatt catctttact
      841 ggatgcggaa aactggtaaa gaaaatcctc tctatgagca ttttgatgac cttttagaaa
      901 ttgctcatca ctatgacgtg actttgagtt taggagatgg cctgagaccg ggaacgattc
      961 tcgatgctat agatggcccc gagattgagg aactcatgat acttgctgag ctatcaaaac
     1021 gagcaagggc aagaggagtt caggttatga ttgaaggtcc ggggcatgtg ccatttactc
     1081 atattcagtc gcatgtgcag cttgaaaagc gtttatgtga gggagcgcct ttttatgtgc
     1141 tgggacctat ccctactgat attgctcctg gatatgacca tattacttcc gcaataggtg
     1201 gagcattagc agcatcagca ggtgcagatt ttttgtgtta tgtgacgccg gctgaacacc
     1261 tcagtttgcc ggatgaaaat gatgtgtatc tgggtgttat ggctgcaaga gttgccgctc
     1321 acgtgggaga tatagctaaa ggtgtacccg gagcgataga aaaagataaa agaatttcct
     1381 tagctcggga gcatctagat tgggaaggga tgatggccga ggctttagac ccggagctgg
     1441 tacgctgcaa atttaacctg gcttccggcc ggcagtcctg taccatgtgc ggtaaacttt
     1501 gtgctgtgaa aatgactcgt ggagcatatg aattataaaa ggagtgatag aaatgaaact
     1561 ggacgaagtt agtataacta aagcaatagt agagcgttat tttgagagat taatgaataa
     1621 cttagagatt gatgtagcta ttgtaggtgg tggaccttcg gggcttgtgg caggatattt
     1681 cctggctaaa gcaggctgcc gggtagtact ttatgagaga aagctcagtg tgggtggagg
     1741 aatgtggggt ggaggaatgc tatttaatga aatagtggtg caacaagatg cccgacgtct
     1801 tttagacgag ttggaagtgc ggactacccc ctataaggaa cctggttact acacagcttc
     1861 ttcggtagaa agtgtgagta ctataacgag caaagcagta aaagccgggc tttcaatatt
     1921 taactgtgtg agcgtggaag atgtagtggt aaaaaatgat agaatttcag ggctggtcat
     1981 caactggaca gctgtttcta tggctggttt acatgtagac ccgctttctg tgagatctcg
     2041 atttatcata gatgctacag gacatgacac tgaagttgtg gctatgatag agagaaaggc
     2101 tccagggaag ctggctactc cctcaggtaa taaggaagga gaaaaattta tgaatcctga
     2161 ggaagcagaa aagttgactc tagaaaatac cagagaggta ttttccggtc tttatgtgac
     2221 ggggatggcc tgtaatgcta cttttggtgg cccacgaatg gggccgattt ttggaggcat
     2281 gcttctctcc ggggagaaag ttgctcaatt gattttaaaa gagttttctc gatgaggtag
     2341 gtggctagtt taatatataa aaagattgac ggaagcggtt tgccttgtta taattctttt
     2401 tgagccgggc tggtggaacc aggaagacac gctatcttga gggggtagtg ggtttttgcc
     2461 cgtgcgggtt caagtcccgc gcccggcacc acctgagaag cacagaggtg gtaaagcggt
     2521 attgaaaaaa gagccggttg agaggtcgtc taaaggtagg actcgtgact ctggatcacg
     2581 cagtggtggt tcgaatccac ccctctcagc cagattttct gttaacctca aagagttgca
     2641 ggtatctcct tccttctcgg taacctttgc ctttttcctc ttcacttgaa aagagagggt
     2701 agcttcagaa tacaaaaata ttattcttgc agagatagaa aaaaggggat tatagtagtt
     2761 gctccctatt atttggaaag cttcggtaat tttaaagaga aagtcttttt gctgttttag
     2821 ctctatacat atcagtatct gctaaagaga agagcttctc ttcatcttct gcatccagag
     2881 gataagaagc tataccaata cttacactca gagggatttc tgttccatct gg
//
LOCUS       DAOSVE010000140         2871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_14061_length_2871_cov_68.026634, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000140 DAOSVE010000000
VERSION     DAOSVE010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14061_length_2871_cov_68.026634"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(332..1126)
                     /locus_tag="PK016_08370"
     CDS             complement(332..1126)
                     /locus_tag="PK016_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HXL03106.1"
                     /translation="MEILRFAFMQRALSIGLIIGALGAVLGVFVVLKRLSFMGAGISH
                     AAFGGIALGYFLGWNPILTAVIFCISVGIGISMLIENNRIKEDTAVGIFFTSTMALGI
                     LLIGLKKEYNIDLFGYLFGNILAVSGEDLVISLIIGAIVAFSLYFFFKELTLSILDPE
                     MARVIGLPVSFLNYLLVSLIALFIVISTRIVGIILVSALLTIPAATALRWSKNLKKAL
                     IISIIDSLGCTIGGLILSYYLDTAPGATIVILATLIFFITLFFNNK"
     gene            complement(1131..1916)
                     /locus_tag="PK016_08375"
     CDS             complement(1131..1916)
                     /locus_tag="PK016_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HXL03107.1"
                     /translation="MEEPIIEVRNVSFFYPPLEVLEGINLTVKKGEFLAIIGPNGGGK
                     TTLLKIILGLLKPQKGEVYIAGKKVEELGKWRSKIGYVPQTAQIDYTFPITVEDFVMT
                     GRFGLIGLGKRPQPSDWEMVRKALKEVKMEEWSKKQIGELSGGQRQKILIARSLALEP
                     EILILDEPTTAVDPESSESFYELLLRLHQQGITIITVSHDVGVVAQYADSIACLNRRL
                     IKHDRPEEVIREGVLEEMYGKEAAFFHHGTIPHIVVQKTSPER"
     gene            complement(1919..2773)
                     /locus_tag="PK016_08380"
     CDS             complement(1919..2773)
                     /locus_tag="PK016_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HXL03108.1"
                     /translation="MKKYFKLALGVLVLVCSFSPFSWGEKLQIGASIAPLCSLVQEVG
                     GDKVEVLQLIPNGASPHTYEPRPSDIRKIEEGQASFLIGLGLDFFLEEIIKNVAGDKP
                     IFYVYQGIDLITEGEGVNPHIWLSLSNARIMVENISQALSSLDPESSAYYAENAREYN
                     QKIAELDRWFKEEVKKLSHRSFVAQHPAWEYLARDYNLELKGVIEKSPGGEPSSREFK
                     NLIEQMEKNDVRVIFAEPQLNQKIAQVLAQETGAQVILLDPLGHFPEQPYLELMRENL
                     NKILEAMR"
BASE COUNT          830 a          619 c          544 g          878 t
ORIGIN      
        1 gcattatgat tgctctatat acattataga acaccaactt ttggtctgtc aacctagttt
       61 gaaacggttc gaaaattatt ctctttcgcg cctattactt caaccccata ctcatttttg
      121 tcatcatgag aagcaaatag ccatggtgct cttgcctttt aatgagcctc acaaaactaa
      181 aaagtgagtt acattggtct aaaaacctgg attgccaggt actgaggaag acgttttttt
      241 cttcaacgtt atagctgagg aaatagaaga ctatgaaaga agtggccgag gcgttaatat
      301 tagcctggga attaaaaaag aaagaataga gtcatttatt gttgaaaaat aaagtaataa
      361 agaatatcag agtggccaat attacaatgg ttgcgccagg agcggtatct aaataatagg
      421 atagaatgag ccctcctata gtgcacccta agctgtcgat gatagaaatg attaaagctt
      481 ttttgaggtt cttgctccat cgcagggcag tagcagcagg aatagttaaa agtgctgaca
      541 ccagtataat ccccactatt ctggtagaga tgacaataaa aagagcaatt aaagagacga
      601 gaagatagtt tagaaaactg actggcagtc caatcactcg agccatttcc gggtctaata
      661 tggaaagagt tagttctttg aagaagaagt agagagaaaa ggcaactatt gctcctatta
      721 ttaaagatat tactaagtct tctccactta ctgccagtat gtttccaaat aagtatccaa
      781 aaaggtcgat attgtattct ttcttcagtc caatcaagag tattcccaga gccatggtac
      841 tggtaaaaaa aatccctacg gcagtgtctt cttttatccg gttgttttca ataagcattg
      901 atattcctat tcccaccgag atacaaaaaa ttaccgcggt taaaatgggg ttccatccca
      961 gaaaatatcc taaagctata cctccaaaag cagcatgaga tattcctgct cccataaagg
     1021 aaagtctctt caagactaca aaaaccccga gcactgctcc taatgctcct attataagtc
     1081 cgatggataa agctctctgc atgaaggcaa atcgtaaaat ttccaagttt tcacctctcc
     1141 ggggatgttt tctgtactac gatatgggga atagtaccat ggtggaagaa tgctgcttct
     1201 ttaccataca tttcttctaa cactccttct cttatcactt cttccgggcg gtcgtgtttt
     1261 attaatcttc ggttcaaaca ggcaatacta tcggcatatt gagccactac tcccacatcg
     1321 tgggaaacag taatgatggt tattccttgc tggtggagac gaagaaggag ttcataaaaa
     1381 ctttcgctac tttccgggtc tactgctgtt gtgggttcat ctaaaattaa tatctctggt
     1441 tcaagagcta gggagcgagc aattaaaatt ttctgtctct gccctccaga aagctctcca
     1501 atttgctttt tgctccactc ctccattttt acttctttca gggcttttct caccatttcc
     1561 caatcagagg gctgaggcct tttccccaga cctattaacc caaatcttcc ggtcattaca
     1621 aagtcttcta cggtgatggg aaaggtatag tcaatctggg cagtttgggg gacatagcca
     1681 attttacttc tccactttcc taactcttct actttttttc ctgcaatgta tacttcgcct
     1741 ttttgaggtt tcaaaagccc caggattatt ttaagtaaag tggttttccc tcctccattg
     1801 ggcccgataa tagccagaaa ctctcctttt tttaccgtaa ggtttatgcc ctccagcacc
     1861 tcaagtgggg gataaaaaaa agaaacattt cgtacttcta tgatgggctc ttccattttt
     1921 atctcatggc ctccagaatt ttattcaggt tttctctcat aagctcaaga tagggctgct
     1981 ctggaaaatg ccctaaaggg tcaagaagaa tgacctgagc accagtctcc tgggccaaca
     2041 cttgggctat cttttgattg agttggggtt cagcaaaaat cactcggaca tcgttctttt
     2101 ccatttgttc tatcaaattt ttgaactctc tagaagaagg ctctcccccc ggggattttt
     2161 ctattactcc ctttagctcc agattatagt ctcgagctaa atactcccag gcgggatgct
     2221 gggcaacaaa agaacgatgg gataattttt taacttcttc cttgaaccat ctatctaatt
     2281 ctgcaatttt ctggttatac tctcgagcgt tttctgcata ataagcgctg ctttccgggt
     2341 ctaaactaga tagtgcctgg cttatatttt ctaccattat tcgggcatta gaaagagaaa
     2401 gccagatatg agggttaacc ccttctcctt ctgttatgag gtctatcccc tggtatacgt
     2461 aaaaaatggg tttatctcct gctacatttt taattatttc ttccagaaag aaatccagac
     2521 ctaatccaat gagaaaagaa gcctgtcctt cttctatctt tctaatatca gaggggcgag
     2581 gttcataagt atgagggctg gctccattgg gtatgagctg gagcacttcc actttatctc
     2641 ctcccacttc ctgaaccaaa ctacaaagag gggcaatgga agctcctatt tgtagttttt
     2701 ccccccagga gaaaggagaa aaactgcaaa ccagaactaa aaccccgagg gccagcttaa
     2761 agtatttttt caaactcttt acctcctgag ctgatttgta attataaaaa ttatagcatc
     2821 ctggagaagt agctattaag agtttaaact atagtatctc gcaaaacctg t
//
LOCUS       DAOSVE010000141         2849 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_14198_length_2849_cov_71.043665, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000141 DAOSVE010000000
VERSION     DAOSVE010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14198_length_2849_cov_71.043665"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PK016_08385"
     CDS             <1..561
                     /locus_tag="PK016_08385"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HXL03109.1"
                     /translation="RYGGLFVFYVVLSRIEWLCFHAMAQAPGGLLTHSYDHSMKAIYL
                     NGILVGEIGMEAAIAGYYHIPLILVTGDSEGVKEAKSLLGEIETATVKYAINEHSALC
                     LPLTVTHKEIKEKAKRATKNINQFAPFRLDPPYAIEVEFFQKEGTQRMEGLPGLQKIN
                     EVKVRVEGENLPLLWESFIGFYQQYD"
     gene            571..1608
                     /locus_tag="PK016_08390"
     CDS             571..1608
                     /locus_tag="PK016_08390"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HXL03110.1"
                     /translation="MKAIVKTKKEKGLELKEVETPRPSRGEVLVKIKAAAICGSDIKI
                     YKWDSFAQSIIHSLPFIPGHECAGEVVEVGEGVKNIQVGDKVASETHIPCGWCWQCTH
                     GRPHTCENMKLFGHNVNGCFAEYAVIPEVSTRKIPSSLTFEEGCMLEPMGIPYRAVEK
                     GEVEKEAVVVVGCGPIGQFAIGFSHAMGAGQIIAVDVNEERLKIAKAVGAHYLLNPEK
                     EKVKERIKELTQDWGKGPGVVIEASGNVSALREALDYLRVGGKLLILGQSTNPLEVRV
                     SPDIVFKETEIYGFWGRKIWDTWEKTEKLLVEGKMKVDPVITHRFSLDEYEIAFEKAV
                     KGEGGKIVFLP"
     gene            1886..>2849
                     /locus_tag="PK016_08395"
     CDS             1886..>2849
                     /locus_tag="PK016_08395"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL03111.1"
                     /translation="MAELGNKYLWMQSHTDAAWLWQWPETVWVVDKTLNHNIKFMNQY
                     AQYKFHYSSPQFYEWMKVIGKYAISSAQGQIFDKDIFNKIVEKVAQGQLIPVDGNWIE
                     PDLNNASGEGLVRQRLYGVRWFKETFGDLPYYKVVWIPDSFGFPHSLPQIYKKSGAEY
                     FTTTKLMWISDTDKAHFPFYVFKWRSPDGSEILSYQTPLGCNQDPQDLEDSQLKREFD
                     FDKARLLLPEYQNKVFNTRNSVNIIKYFDDKYINDMAILAGKGDHGEGPTEEEIKAAI
                     ALAGKHGYFISTANDFFDVLEKNYDTSLIPVWDDELYVETHRGTY"
BASE COUNT          914 a          458 c          701 g          776 t
ORIGIN      
        1 cgctatggtg gtctttttgt cttttatgtg gttctaagcc ggatcgagtg gctatgtttc
       61 catgctatgg ctcaagctcc gggagggttg ttaacccatt cttatgacca ttctatgaaa
      121 gccatatatt tgaatgggat tctggtggga gaaataggta tggaagcagc aatagccggc
      181 tattatcata tacctttgat tttagttact ggtgattcag aaggggtgaa agaagctaaa
      241 agtcttctgg gagaaataga aacagcaacg gtaaaatatg ctataaatga acatagtgct
      301 ctgtgcttgc ctcttacggt aactcacaag gaaataaaag agaaagctaa aagagcgaca
      361 aaaaatatta accagtttgc cccttttcga ttagaccctc cttatgctat agaagtggag
      421 tttttccaaa aagaaggaac gcagaggatg gaaggattac cggggttaca gaagattaat
      481 gaggtaaaag ttcgggtgga gggggagaat cttccccttt tgtgggagag ttttattgga
      541 ttttatcagc aatatgattg agaggtaaat atgaaagcca ttgttaaaac caaaaaagaa
      601 aagggactag aattaaaaga ggtggaaact cctcggccaa gtagaggaga agttttagtg
      661 aaaataaaag cagctgctat ctgtggctca gatataaaaa tatataaatg ggattctttc
      721 gctcagagta taattcattc tcttcctttt attcccggtc atgagtgtgc gggagaagtg
      781 gtggaagtgg gggaaggagt gaaaaatatt caggtgggag ataaagtggc ttcagaaact
      841 catattccct gtggttggtg ctggcagtgc acccatggta gacctcatac ctgtgaaaat
      901 atgaagctct tcggtcataa tgttaacggg tgctttgctg aatatgcagt tatccccgag
      961 gtatctacca gaaaaatccc ttcctctctt acttttgaag aaggatgtat gctagagcca
     1021 atgggaatac cttatcgggc agtagaaaaa ggagaggtag aaaaagaagc agtggtggtg
     1081 gtagggtgcg gtcctatcgg tcaatttgct ataggctttt ctcatgcaat gggggcgggt
     1141 caaattattg ccgtggatgt aaatgaagag aggctgaaaa ttgctaaagc tgtgggcgct
     1201 cactatctcc tgaaccctga gaaggaaaag gttaaagaga gaataaaaga gttaacccaa
     1261 gactggggaa aaggaccggg agtggtaatt gaggcttcgg gaaacgtttc ggctctgcgg
     1321 gaggcgctgg attatctccg ggtagggggc aaacttctca tcttaggtca atctactaac
     1381 ccattagaag tgagagtgtc tccggatata gtgtttaaag aaacagagat ttatggtttc
     1441 tggggaagga aaatctggga tacctgggag aaaacagaaa aattgctggt agaggggaaa
     1501 atgaaagtag acccggttat aactcatcgc ttctccttag atgagtacga aatagctttt
     1561 gaaaaagcag taaaaggaga aggaggaaag attgtttttt taccttgaaa ggcacaatta
     1621 gctgcaaaac ttatggtaat attctttttg aatatggtta ttgagaactc gtaatttcat
     1681 ttacgtggcg atcgcggggt tttatgctat cttgcttgtc gttcccgagc gctttagtcg
     1741 ggaatctaga tgtttaacca ttaaccacta tagacccccg gctcgaaagt tgccggtgtg
     1801 acgaagggag taataagaat attgctctac tggagatcca agagctaaaa agaggagaaa
     1861 tgaaagcttt caaggaggaa ataaaatggc agaattaggg aataaatatt tatggatgca
     1921 gtctcacact gacgctgcgt ggctctggca gtggccagaa acagtatggg tagtggataa
     1981 aacgttaaat cacaatataa aatttatgaa tcaatatgct caatataaat tccattatag
     2041 ctctcctcaa ttctatgaat ggatgaaggt aattggtaaa tacgctattt cctcagcaca
     2101 aggacaaatt tttgataaag atatatttaa taaaatagtg gaaaaagtag cacagggtca
     2161 attaatacca gtagatggta actggataga gccagactta aataacgctt caggggaggg
     2221 tctagtaagg cagcgtctgt atggagttag atggttcaag gagacatttg gagatttacc
     2281 ctattataaa gtggtctgga taccggacag ttttggtttt ccccactctc tacctcagat
     2341 atataaaaag agtggtgcag agtatttcac taccacaaag ctaatgtgga tatcagatac
     2401 tgataaagct cattttcctt tttatgtttt taagtggaga agtcctgatg ggtcagaaat
     2461 tctttcctac caaaccccct taggatgtaa tcaagaccct caagatcttg aagattctca
     2521 acttaaaagg gaattcgatt ttgataaagc cagattacta ttgccagaat accagaacaa
     2581 agtatttaac accagaaata gtgttaacat aattaagtat ttcgacgata aatacataaa
     2641 cgatatggct attctggccg gaaaaggtga ccatggagaa ggccccaccg aagaggaaat
     2701 aaaagctgcc attgctttag ctggaaaaca cggttacttt atttctacag caaatgactt
     2761 tttcgacgtc ctggagaaaa attatgacac cagtttaata ccggtgtggg atgatgagct
     2821 atatgtggaa acgcaccggg ggacatata
//
LOCUS       DAOSVE010000142         2844 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_14237_length_2844_cov_71.352815, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000142 DAOSVE010000000
VERSION     DAOSVE010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14237_length_2844_cov_71.352815"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..996
                     /locus_tag="PK016_08400"
     CDS             136..996
                     /locus_tag="PK016_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL03112.1"
                     /translation="MAKASIEKRVDRLEEALTELAQQSRVTEIRLATFIQEMQDYREE
                     NDRMIQEMKEENNRMIQEMKEDTRLLGERLDKTIQEMKEENNRMIQEMKEDTRLLGER
                     LDKTIQEMKEDTRIFREGVKVYQDRMDKTVQDMNRRWGELANKMGTVTEDIVAPGFPF
                     LIERYFGLSLDDISCRRKVKDKSGNRWEFDLVARSGNIAFIVDVKSDYNRVEYLDHFT
                     QVVLPKATELLPDLKGCKIIGCIASFRLTPSIIERASQMGILAISLGGDYLEFCNQEV
                     VESIREAIRG"
     gene            1428..2678
                     /locus_tag="PK016_08405"
     CDS             1428..2678
                     /locus_tag="PK016_08405"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HXL03113.1"
                     /translation="MENQKEQQKSVVLLIIFLLALVVRSLLASFIPGYITDVGCFKGW
                     ADQVAKVGFSRFYSSDMFVDYPPGYILVLYPIGKIRLLFNLDFNSPLFLFLIKLPSLI
                     TDLASGYLLLSAARKAKLNFSLSLLLILYLFNPAILINSAVWGQIDSFFTFFVLLSLW
                     FIEKKRFSVASATMVYALLVKPQALFFFPILFFALLENKNWKTFFKSVVSGLAMLLLV
                     ILPFSWGNGISWLVQKYQETISSYPYASLNAFNFFALTGGNWVEETRPFLFLNYKIWG
                     YIFLLIILGIATFLFFRWKNPYKIYPLSFFLISSIFMFSSKMHERYLYPALIFALFTF
                     VYFPYRRVLLTYSGFSITHFLNVGLVLLYSWYNVFHLYRYSPYVLAISLANLLLYGYA
                     LYTGYHLYHNEKSVVIEERSFISS"
BASE COUNT          847 a          544 c          557 g          896 t
ORIGIN      
        1 aaaaaaagcc cttgacggga gaggactaag gtgagggtgt ctttccctca agaaagtagc
       61 gtgtctcctt ttccatccct ttccggaagt taattttatg ctataatgaa gaaaactata
      121 aaaaggtgaa ataatttggc taaagcctct atagaaaaaa gagtagatag attagaagaa
      181 gcccttactg agttagctca acaatctcgg gtcacagaga taagattagc tacttttatc
      241 caggagatgc aagattaccg agaagagaat gaccggatga ttcaggagat gaaagaagaa
      301 aataatcgga tgattcagga gatgaaagaa gataccagat tgctgggaga aaggctggac
      361 aaaaccattc aggagatgaa agaagaaaat aatcggatga ttcaggagat gaaagaagat
      421 accagattgc tgggagaaag gctggacaaa accattcagg agatgaaaga agataccagg
      481 atattccgag aaggggtaaa agtctaccaa gacagaatgg ataagactgt ccaggatatg
      541 aatcgcagat ggggagagtt agctaataag atgggaactg taactgaaga tatagtagct
      601 cctggctttc cttttctcat agaaagatac tttggtttat ctctggatga tataagctgc
      661 cggagaaaag taaaagataa atctggtaat agatgggaat ttgatttagt agccagaagt
      721 ggaaacatag cttttattgt tgatgtaaaa agtgactata atcgagtaga atacttagac
      781 cattttactc aagtagtctt acctaaagcc actgaacttc ttcctgacct caaaggatgc
      841 aagattatag gttgtattgc ttccttcaga ctaactcctt ctatcattga gagagcttcc
      901 caaatgggta tcttagccat ttctttagga ggagattact tagagttttg taatcaggaa
      961 gtagtagaga gtataagaga ggctataagg gggtaaaccc cactgagaga gactactgag
     1021 gaagacaaag ctttcctcag tggattcctg ataaaagatt ccaggcatga cggaagggga
     1081 agcccttgaa gggagacccc ctatttttcc tttccctcat ttttagttag agtaaaggtg
     1141 aagtttccac aataatctta tttgcaacag cttacccctc tccttatctc tcccgcaaaa
     1201 atagtaagaa aatccgggtt gtcacggaag gaattacgac cctcacgatg acaccccgga
     1261 ataaattgcc atagtaatgc cctggcacaa aaaatgaata gagcataaaa agaaacatta
     1321 ccatggattt tgcagttaac tatggtcagc tacaaattac atggtaaaat aacataaaag
     1381 tgtaaataaa cctatcaagt actatctatt ttctaatgag ggatgaaatg gaaaatcaga
     1441 aagagcagca aaaatctgtt gttttactta taatttttct gttagcctta gtggttcgat
     1501 cccttcttgc ttcttttatt cctggatata ttactgatgt agggtgtttc aaagggtggg
     1561 cagatcaggt agcaaaagta ggattttccc gattttactc cagcgatatg tttgtagact
     1621 accccccggg ctacattctg gttttgtacc ctatcgggaa aattcgactg ctgtttaatc
     1681 tggatttcaa ttcccctctt tttctgttcc tgataaaatt gccttctttg attaccgacc
     1741 tggctagcgg ctatttgctc ctctctgccg ctcggaaagc taaacttaac ttttctcttt
     1801 ctctcctttt gattttatat ctctttaacc ctgctattct tatcaattcc gctgtttggg
     1861 gacagattga ttctttcttc accttctttg tacttctttc tttgtggttt atagagaaaa
     1921 agcgtttttc tgtcgcctca gccactatgg tgtatgctct ccttgtaaaa cctcaagccc
     1981 ttttcttttt ccctatttta ttctttgcgc tcttagaaaa taaaaactgg aaaacctttt
     2041 tcaagagtgt ggtttctgga ttagcgatgc ttcttctagt gattcttcct ttttcctggg
     2101 gaaatggaat ctcctggttg gtgcagaaat accaggaaac tatttcttct tatccttatg
     2161 cttcactcaa tgcttttaat ttttttgccc tgactggagg aaactgggta gaagaaactc
     2221 gcccctttct ttttctcaac tataaaatct ggggctacat ctttctatta attatattag
     2281 gaattgcgac ttttctattt ttccggtgga aaaaccctta taaaatctat cccttaagct
     2341 ttttcctcat cagttctatt tttatgttca gttctaaaat gcatgagcga tatttatatc
     2401 cagcccttat ttttgctctt ttcacttttg tttatttccc ctaccgccgg gttttactca
     2461 cttattcagg atttagcata acccatttcc taaacgtggg attagtgctt ctttatagct
     2521 ggtataatgt ctttcatctg tatcgatata gtccttatgt tttagccatc tccttggcta
     2581 atctcctttt atacggttat gccctctaca ctggctatca tctttatcac aatgaaaaat
     2641 cagtagttat tgaagaaagg tcgtttatat cctcttagcc actcagaatg ctccagaaca
     2701 ataaggggct ttactccctt atcaaccccc aaaaagcaaa aaactactga ggaaggaaaa
     2761 gcctccctca gtggattccc gcctaaattt cgggaatgac aaagagaggg atatcattcc
     2821 tgaatggttc tatcaggaat ctaa
//
LOCUS       DAOSVE010000143         2823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_14357_length_2823_cov_85.123916, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000143 DAOSVE010000000
VERSION     DAOSVE010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14357_length_2823_cov_85.123916"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            755..1300
                     /locus_tag="PK016_08410"
     CDS             755..1300
                     /locus_tag="PK016_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXL03114.1"
                     /translation="MLESLITSQTRIKLLLKFFLNPESKSYLRELAEEFGESTNSVRL
                     ELNRLTQAGLLTSAENGRTKVYQANPNHPLFPEIQSMVRKFTGIDQLIPQVLSQLGEI
                     HSAFVTGDYARGIDSGIIDLVIVGKVDELYLKKLIDKAEQLIHRKIRFLVLSDEELKN
                     LKDTLKIEEAIPLWGEKGSDE"
     gene            1357..1590
                     /locus_tag="PK016_08415"
     CDS             1357..1590
                     /locus_tag="PK016_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL03115.1"
                     /translation="MAEGLKERLVKELDRLPEERLQEVLDFVEYIIVKESRSLTSKPL
                     EELDPEKDPILKFVGGVSHGSLAKDIDEELYEG"
     gene            1587..2009
                     /locus_tag="PK016_08420"
     CDS             1587..2009
                     /locus_tag="PK016_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010474731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HXL03116.1"
                     /translation="MKLFIDTWGWLSLRDRREMRHEEAKNFYYSFRRQSGKAFTTDYV
                     LDETFTLIFKRLPLLQAHESLVMLDEAIKKGYLWLEWITPERFEQAKRLRIKFQDKPR
                     ISFTDLTSIVVMDELGITDILTEDVHFTQVGIGVRIIP"
     gene            2090..2260
                     /locus_tag="PK016_08425"
     CDS             2090..2260
                     /locus_tag="PK016_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL03117.1"
                     /translation="MMADRGLDKEVKKALMTIKYTKHAETSLRERKNGKDVVDGPQRS
                     RNIGRENDENKI"
     gene            2244..2474
                     /locus_tag="PK016_08430"
     CDS             2244..2474
                     /locus_tag="PK016_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HXL03118.1"
                     /translation="MKIRYDEEADALYIKLREAEYYESDEIREGFILDYDADGNIIAI
                     EILEVSTHVPLADLSTMNFEINRQLMKIGSDG"
BASE COUNT          806 a          511 c          689 g          817 t
ORIGIN      
        1 tgagcactct cctttaattt actccccttt tattatacca gactacctgc ggtgcaggct
       61 gtctgttttt tccgggggat tataaagggg cacagctttc ttatagcctt caccctcacc
      121 ctaaccctct cccttcaagg gcgagggaag aaaaagaaag agggttcttt ccccctttgt
      181 accgagggac gttttttcct cggttagtat ttatcttttc gtgttttgtc tttaggtttt
      241 tgtattttgg gggttatgag gggcttttta cccactgagg gagacgtttt ttctccctca
      301 gtaatctttg ctttttaagt ttttatcttt tgtcttttag tcttttgttt ctgggggtta
      361 ttagggggta ttttccccct aatagttctg ttctttttaa tcctacgccg taggcacgct
      421 gggacgattt tccccagcgt gattatcaag attttttctt atctaagttg cttttaatct
      481 tacgccgtag gctgtctgtt ttttccgggg gattataaag gggagaaccc ctttatagtt
      541 tgaccactca ggaatgacgg ataggaggga atttcaccct cactctcact tcccccccgg
      601 aaggatgacg aaggggggtt tccccttatt gctcttgaca ctcctctttt cgagtagtat
      661 tattactcac aaaggtaaat aattatttct aattgcctgg caacctgggc caggactcag
      721 ttttttagat aaagtaggtg ggttattgaa attaatgtta gagtctttaa tcacttctca
      781 aaccagaata aagctcttgc ttaaattctt tctcaacccg gagagtaaat catatctgcg
      841 ggagctggct gaggagtttg gggagtctac taactccgtt cgcttagagc tcaatcggct
      901 tacccaagct ggtcttctca cctccgcgga aaatgggagg actaaagtat atcaggccaa
      961 ccccaaccat cctctttttc ctgaaatcca gagtatggtg cgcaaattca ctgggattga
     1021 ccagctcatc ccccaggttc tctcccaatt aggggagatt cattctgctt ttgtaactgg
     1081 tgactatgcc cgggggatag actctggtat cattgacctg gtcatagtag ggaaagtgga
     1141 tgagctttac ctaaagaagc ttatagataa agcagaacag ctcatccatc gcaaaatccg
     1201 ttttctggtt ttaagtgatg aggaactaaa aaatcttaaa gatacgttga aaatagaaga
     1261 ggcaattcct ttgtggggag agaaaggcag tgatgagtga gagtgagtgg tgaaaaacct
     1321 gactgaaacg gtggattgaa aggagggaaa acagtgatgg cagaagggtt gaaggagcga
     1381 ttggttaaag agctcgatcg gttgcctgaa gaacgactac aggaggttct ggattttgta
     1441 gaatatatta ttgtgaaaga aagcagaagc ctgacctcca aacccttaga agaactggac
     1501 cctgaaaaag atcctatatt gaaatttgtg gggggagtgt ctcatggctc tttggccaaa
     1561 gatatcgatg aggagttgta tgagggatga agctttttat cgatacctgg ggttggctca
     1621 gccttcgaga tagacgagag atgcgacatg aggaagcaaa aaacttttat tacagtttcc
     1681 gcagacaaag tggaaaagcc tttactactg attatgtctt agacgaaact tttaccttga
     1741 tattcaaacg attgcctctc ttacaagctc acgagtcatt ggtgatgttg gatgaggcca
     1801 tcaagaaggg ctatctctgg ttagaatgga taacaccgga acgctttgaa caggctaaga
     1861 ggctgcgaat caaatttcaa gataagccac gaatctcatt tacagatctt acctccatag
     1921 tagtgatgga tgagctgggc ataactgata tcttgaccga agatgtgcac ttcactcagg
     1981 taggaatagg cgttaggatt attccttaat catgcggagt aatgagtaac gagtaatcag
     2041 tgaggacgcg taaggcgtga acaagccata gagcatatga tgtgagacta tgatggcaga
     2101 tagaggattg gataaagagg taaagaaggc acttatgacc attaaatata ctaagcatgc
     2161 ggaaacaagc cttagggaac ggaaaaatgg aaaagatgta gtggatggac ctcaaaggtc
     2221 gagaaatatt gggagggaaa acgatgaaaa taagatatga tgaagaagcg gatgctcttt
     2281 atattaaatt gcgggaagca gaatattacg agagtgacga gattcgagag gggttcattc
     2341 tggactacga tgcagatggg aacatcattg ccattgagat tttagaagtt tcaacccatg
     2401 ttccccttgc tgacctctct accatgaatt tcgaaattaa tcgtcaactg atgaaaattg
     2461 ggagtgatgg ttagtgtatt cccgggaggg aggagaaaag cagtgaggag taatgagtaa
     2521 tgcgtaatga gtgagagtaa aagtgagcag ttcgcaactt gacctaaaag ggttagctga
     2581 aaggaggcag agatgccaaa agttaaggat ttaagtatgg atgacttgga atacttgata
     2641 gagcagaagc ttttagaaat agaagcttat aaattacgat ttgattaatg atcgatgccg
     2701 gctttgagac accaccctta gtaattacgc gatgggaaaa gttgcaagtt gggttaatat
     2761 tcccctctaa gcaatttttc aaaatcttcc tgagttatac ccatttgcct caatatgctt
     2821 tta
//
LOCUS       DAOSVE010000144         2789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_14589_length_2789_cov_74.580834, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000144 DAOSVE010000000
VERSION     DAOSVE010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14589_length_2789_cov_74.580834"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PK016_08435"
     CDS             <1..269
                     /locus_tag="PK016_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013429289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXL03119.1"
                     /translation="AFESLPSINCNMCKYCLPCPNGVNIPRNFELYNNAFQGGAFEDA
                     RRKYLVLPEEEKASSCLECGDCESLCPQELPIPELLKKVAQAFE"
     gene            698..2155
                     /gene="hydG"
                     /locus_tag="PK016_08440"
     CDS             698..2155
                     /gene="hydG"
                     /locus_tag="PK016_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HXL03120.1"
                     /translation="MNVQEWMSSIIKEEENEKFLEGGRDFINDEEIEELLRENRNPEP
                     QKVRDIIAKSLSLKRLEPEETATLLNVKDEELWEEMYKAGGEIKRSVYGDRIVTFAPL
                     YCSNFCVNNCLYCGFRRDNNVQKRRRLTLEEVKKETEVLVSLGHKRLVVVYGEHPLSD
                     AYYIASTIQTIYDTRVGNGEIRRVNVNAAPMDVARYKILKEVGIGTFQVFQETYHHET
                     YRQFHPSGIKSNYQWRLYALHRAQEAEIDDVAIGALFGLYDWKFEVMGLLYHTIDLEK
                     HFAGVGPHTISFPRLEPAVNTPLVEETRYRVSDRDFKRLVTVIRLSVPYTGMILTARE
                     PAYIRRDVIPVGCTQIDASTRIGIGAYSDSQSDQELDRQQFQIGDSRSLDEVIRELAE
                     MGYITSFCTAGYRCGRTGEHFMEIAKKGKVHNFCIPNAILTFQEYLEDYASPQTREIG
                     EKVIQNHLSSLKDGIKPKVEEYLQRIKKGERDLRI"
     gene            2133..2441
                     /locus_tag="PK016_08445"
     CDS             2133..2441
                     /locus_tag="PK016_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL03121.1"
                     /translation="MKETYEFEKWTKQIEEKISKLPVEEKLEIMAEEIYSRYGVRVWF
                     SEISGKRWSHIAGRGSYEPAPIHQVLLSPRYGMAIEGDEIPEIEREALLSFIKRELYF
                     "
BASE COUNT          906 a          542 c          603 g          738 t
ORIGIN      
        1 aagcttttga gagtctcccc tctataaact gtaatatgtg taaatattgt cttccctgcc
       61 ccaacggggt gaatattccc cgcaattttg aactttacaa taatgctttt cagggaggtg
      121 cttttgagga tgctcgccga aaatatttag tccttcccga agaagaaaaa gcatcctcat
      181 gtcttgagtg cggagattgt gagagcttgt gtcctcaaga gctccccata cctgagctcc
      241 tgaagaaagt tgctcaagct tttgaataat gagctatagg gggagagaaa aatcccccta
      301 tagctttttt gtcgtcaccc ggcaatcttt aagccggggc ctaaacagca agggagaaat
      361 ctcatacccc agagaatacc agcactctat gagaaaataa accccatgtt gacgtaataa
      421 aattcctcgc aacggtagcc aagtataaat ttatccttaa tggcacaact caaacattca
      481 tcactctacc tgaatatcca agtattattt acactatcca gttagcatat ttatttgaca
      541 tttaagggga ttttatttag tatttatctg gttataattt gaggaacctg cggcgaggaa
      601 ggttttctga aaccaagggt aaaatattta ttaatgcctg gccttacctt agccgtaggg
      661 ccaggcatta ttttttcggg aaaggaaggt aagaaaaatg aatgttcaag aatggatgag
      721 tagcatcatc aaggaagaag aaaatgaaaa gttcttagaa ggtggaagag acttcataaa
      781 cgatgaagaa atagaagagc tcctccggga aaaccgtaac cctgaacccc agaaagtacg
      841 agacatcatt gctaaatcgc tatctctcaa gcggctggaa cccgaggaaa ctgctacttt
      901 gttgaatgtg aaagatgaag aactgtggga ggagatgtat aaagctggag gagagataaa
      961 aagaagtgtt tacggagata gaatagtaac ctttgctcct ctttactgca gtaatttctg
     1021 tgttaataat tgtctatact gcggtttccg ccgggacaat aatgtgcaaa agagacgccg
     1081 attaactctg gaagaagtga aaaaagaaac cgaagtcctg gtttctctgg ggcataagag
     1141 attagtagtg gtttacggag aacaccctct ctctgatgcc tactatattg ctagcaccat
     1201 ccaaaccatt tatgatacca gagtaggaaa tggagaaatc aggagagtaa atgtcaatgc
     1261 tgctcctatg gatgtagccc ggtacaagat ccttaaagaa gtaggaatcg gaacttttca
     1321 agttttccag gaaacttacc atcatgaaac ttatcggcag tttcatcctt caggaataaa
     1381 atccaactat caatggagat tgtatgccct tcatcgagcc caagaggcag aaattgatga
     1441 tgtagccatt ggagcactat tcggattata cgattggaag ttcgaagtta tgggacttct
     1501 ctaccatact atcgacttag aaaagcactt tgccggagtt ggccctcata ctatatcttt
     1561 tccccgcttg gagccagcag taaatactcc tttagtggag gaaactagat atcgagtgtc
     1621 cgatcgggac ttcaagcgat tagttactgt tatcagactt tctgtccctt acacgggaat
     1681 gattctcact gctcgagaac cggcttacat ccgcagagat gtgattccag tggggtgcac
     1741 ccagattgat gcctccactc gcatcggtat tggagcttat agtgatagcc agagtgacca
     1801 ggaattagat cgtcagcaat ttcagattgg agactctcgt tctttagatg aggtaattcg
     1861 ggagttagca gaaatgggat atattacttc tttttgcacc gctggttacc gttgtggaag
     1921 aacaggggaa cactttatgg aaatagccaa aaaaggcaaa gtccacaatt tctgcatccc
     1981 caatgccatt ttaactttcc aggaatatct ggaagattat gcttctcccc aaaccaggga
     2041 gataggagaa aaggttatcc agaatcattt atccagcctt aaagatggaa taaaacctaa
     2101 agtggaagaa tatttacaga ggataaaaaa aggtgaaaga gacctacgaa tttgaaaaat
     2161 ggacaaaaca aatagaagaa aaaattagta aacttccggt agaagaaaaa ctggaaataa
     2221 tggcggaaga gatttattcc cgctatggtg tgcgggtgtg gttcagtgag atttcaggaa
     2281 aaagatggtc gcatattgcg gggcggggta gctatgagcc cgcccccatc catcaagttc
     2341 ttctctcccc ccgctatggt atggcgatag agggagatga aattccagaa atagaaagag
     2401 aagctcttct ctctttcatt aaaagagaac tttattttta gcttcccccg ctattttcgg
     2461 gatttcttct atcagataga aaaacagacg tgctttaatc cccattttct gcaagatgaa
     2521 ctaagagctt attcctttcc ctcgagctac aatcaaaagt cgtgtatagt cagataaaaa
     2581 aacgggtagc gattattgta aagcctcaag aaaatcatct aatttttata gttaactgca
     2641 aaactaatta taaatactat aaaggggagt gcccctttat aatcccccaa aagcaaaaaa
     2701 ttaaaaactg aaaagacaaa aactactgag gaaggaaaaa cgctttcctc agtacctgcg
     2761 gcaccaaaga cgaaagcaaa atagatgaa
//
LOCUS       DAOSVE010000145         2780 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_14650_length_2780_cov_69.460183, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000145 DAOSVE010000000
VERSION     DAOSVE010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14650_length_2780_cov_69.460183"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PK016_08450"
     CDS             complement(<1..257)
                     /locus_tag="PK016_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXL03122.1"
                     /translation="MSLEIVLIEDEKDVVDFVSYFLQKEGYQVFSFLRGEDFLEKMDT
                     LLPDVIILDLMLPGIDGLEVFRYVREYPRFRNVPIIMLTAK"
     gene            complement(381..1214)
                     /locus_tag="PK016_08455"
     CDS             complement(381..1214)
                     /locus_tag="PK016_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009379451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HXL03123.1"
                     /translation="MKKVLLSSLVLCFILSLSGLSWGAEAVDVQGSTTILPIMQIIAE
                     EFMAQNEDIEVTVGGGGSGVGISALLDGITNIVMASRQMKEEEWQKAEEKGLDIREIE
                     IAKDALSVIVNSLNPISEIDIDTLQQIYTGKINNWPDLGVALDGEIVIISRDTNSGTF
                     EVFNEHVLKGEELVDKALRLSSNQAVLNEVTTNSNAIGYVGLGYTQQGGDKIKALTVN
                     GVEASPEKALSGEYPLSRGLYLYVNGEPQGAVKKLIDFILGPEGQTIVEEEGFVPLSL
                     K"
     gene            1333..2742
                     /locus_tag="PK016_08460"
     CDS             1333..2742
                     /locus_tag="PK016_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HXL03124.1"
                     /translation="MAQTFGLIVGNRGFFPDELAKEGRSKILEVLKKKGFEVIAPSTE
                     DTKFGTIETWEDAQKCARIFDENRHRIDGIIITLPNFGDERAAAQTIRLSGLSVPVLV
                     HAFPDQLNALDVQNRRDSFCGKLSICNNLKQFQIPFSLTSYHTEDPDSPIFGEDLEWF
                     AGVCRVVKGFKNLKAGAVGARPAAFNTVRFSEKILESYGISVETIDLSEIIGNMNRLD
                     NNNLEVKRKVQEIKDYIDTTGIPEEALVKMAKLSVVLEEWIKATGVSLFSFQCWNSIE
                     ENYGIMPCTVMSMFSEKLVPAACEVDLVGGLAMYALQLASQKPSALVDWNNNFGDDPD
                     KAILFHCSNFPKSLLRGAKMGYGDIISSSVSKEKAYGTCYGTIPHGEVTLARLATNDE
                     EGNVISYIAEGEITPDQVNTFGGVGVVQIEDLQDLLNYICNNGFEHHVAINLSQSGDI
                     LSEAFENYLGIEAYYHNIY"
BASE COUNT          863 a          590 c          520 g          807 t
ORIGIN      
        1 ctcttagctg taagcataat aattggaacg tttctaaatc gaggatactc tcgaacatag
       61 cggaaaacct ctaaaccatc aatacctgga agcatgaggt ctaaaataat aacgtcagga
      121 agaagagtat ccatcttttc caggaaatct tcaccccgca aaaaggaaaa aacctgataa
      181 ccttctttct gaagaaaata agagacaaaa tcaactacgt ctttctcatc ttctatcaaa
      241 actatttcta aactcaaact atcttttcac cccactatta caaaattctg gcacctttcc
      301 gggggataaa aaggctattt ccccgaggcg ctacttttag taaactatat ggggggacat
      361 tctccccccc cccatggttt ttactttaga gaaagaggaa cgaagccctc ttcttccact
      421 atggtttgcc cctcaggacc caggataaaa tcaatcaatt tcttaactgc tccctggggt
      481 tctccattaa catagagata cagtcctcga gaaagaggat actccccaga aagtgctttt
      541 tcggggcttg cttcgactcc gttcacagta agagctttga ttttatctcc tccctgctgt
      601 gtatatccca atcccacata tcctatagca ttggaattag tggtaacttc attcagcact
      661 gcctggttag aagaaagacg taaagcttta tcaacgagtt cttccccttt caggacatgt
      721 tcattaaata cttcaaaagt cccggaatta gtatcccgag aaataattac tatttccccg
      781 tctaaagcaa ctcctaaatc tggccagttg tttatttttc cagtgtatat ctgttgcaag
      841 gtatcaatat caatttccga aataggatta agagagttaa ctataaccga aagggcatct
      901 ttagctattt ctatttcccg aatatccaga cctttctctt cagccttttg ccactcttct
      961 tctttcatct ggcgggaagc cataactata ttggttattc catctaaaag agcagaaata
     1021 cctactcctg aaccacctcc acctacggtg acttctatat cttcgttttg agccataaat
     1081 tcttcagcta ttatctgcat aataggtaaa atagtagtgg aaccctgcac atcaaccgct
     1141 tctgctcccc agcttaagcc ggaaagagag agaataaaac ataacactaa gctacttaac
     1201 agcacctttt tcatttatct tacctccctt tttaattttt tattctgaaa atatggtaag
     1261 ataagagtgt taaatcggta tttaaataat attaaacttt tttaatattt taacatgaaa
     1321 ggagaaataa gtatggcaca aacttttggt ttaattgtag gtaaccgagg tttcttccct
     1381 gatgaattag ctaaggaggg aagaagtaaa attttagaag ttctaaagaa aaaaggtttt
     1441 gaagtaatag cgcccagcac tgaagatacc aaatttggta ccatcgagac ctgggaagat
     1501 gcccagaaat gtgcccgcat ctttgacgaa aatcgtcacc gcatagacgg gattataatt
     1561 accctaccca atttcggtga tgagcgagca gccgctcaaa ccattcgtct ctcaggatta
     1621 agtgttccag ttttagtaca tgccttcccc gaccagctta atgctctgga tgttcaaaat
     1681 cggagagata gcttctgtgg caaactctcc atatgcaata acctgaagca atttcagatt
     1741 cctttttctt taacttctta ccatactgaa gaccctgatt ctccaatctt cggagaggat
     1801 ttagagtggt ttgcaggagt atgtcgagtg gtaaaaggat tcaaaaatct aaaagccgga
     1861 gcagtgggag cacggccagc tgcttttaac accgttcgtt tctcagaaaa aattctggag
     1921 agttatggaa tatcagtaga aaccatagac ctctcagaaa taatcggtaa tatgaataga
     1981 ttggataaca acaacttaga agtgaagaga aaagtacagg aaataaaaga ttatatcgat
     2041 actaccggga ttcccgaaga agcgctggtc aaaatggcta aactttctgt ggtattagaa
     2101 gaatggatta aagcaaccgg tgtatctcta ttttcttttc agtgctggaa ctctatcgaa
     2161 gaaaactatg gcattatgcc ttgtacagtg atgagtatgt tcagcgaaaa gttggtaccc
     2221 gcagcctgcg aagtagacct ggtgggagga ttagctatgt atgccctgca gctcgcttct
     2281 caaaaaccat ctgctcttgt ggactggaac aacaattttg gcgatgaccc cgataaagct
     2341 attcttttcc actgcagtaa ctttcccaag tcacttctgc ggggagctaa aatgggatac
     2401 ggtgacatta tctcttcctc agtttctaaa gaaaaagctt acggaacttg ctatggaact
     2461 atacctcatg gagaggtcac tctggctcgt ttagctacca acgatgaaga gggtaatgta
     2521 atatcttaca tagcagaagg agaaattact cccgaccagg tcaatacttt tggaggagta
     2581 ggagtagtgc aaatcgaaga cctccaagat ttattgaact acatttgcaa taacggtttt
     2641 gaacatcacg tagctataaa tttatctcaa agcggagata ttttgtctga agcttttgaa
     2701 aactacttag gaatagaagc atactaccac aacatctatt aattcccaaa gagggaagct
     2761 taagcttccc tctttgtttt
//
LOCUS       DAOSVE010000146         2730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_15013_length_2730_cov_71.219065, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000146 DAOSVE010000000
VERSION     DAOSVE010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15013_length_2730_cov_71.219065"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..1201
                     /gene="hydE"
                     /locus_tag="PK016_08465"
     CDS             125..1201
                     /gene="hydE"
                     /locus_tag="PK016_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HXL03125.1"
                     /translation="MENSGKEWDLLNKLDQEEDLAQEEIVILLDCTDRKIEEQLFHLA
                     DEKRKKYVGEEVHLRGLVEFSNFCRRNCLYCGLRRGNRKLKRYRMSPEEIYNLAEKIA
                     LQGLKTIVLQSGEDLTYDARALASLIHRIKKLDVAVTLSIGEREPWEYALWRDAGADR
                     YLLKQETFDRELFAKLHPDDDFDQRLECQKTLKELGYQVGSGNMVGLPYQSLHALALD
                     IKKFQEWDFDMIGIGPFIPNPDTPLGEEISNSEEKYILTLKVLALTRILTRTSNLPAT
                     TALGALTSGGREKALSCGANVFMPNLTPSFYQKRYIIYPGKGIEESWENYLLEVKKMV
                     EREGRIVSTGYGDRISLSGSGEAI"
     gene            complement(1234..2451)
                     /locus_tag="PK016_08470"
     CDS             complement(1234..2451)
                     /locus_tag="PK016_08470"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HXL03126.1"
                     /translation="MAEVLENLKSLLKKAESKIVLLIADGLGGVPHPDFGYQTELEAA
                     RTPNLDELAKKSVLGLMTPVNYGITPGSGPGHIGLFGYNPEELIIGRGVLEALGVGFH
                     LQAGDIAARANFATRDEKGIIVDRRAGRISTEECTRLVEKISQEIKEIEGVEIILKPG
                     MEHRFVLILRGEGLGENVRDTDPQKEGLPPYEPQALDAGAEKTARVLKLFLEKANQII
                     KKEPKANAILLRGFSQSPHLEKFPERYGVKALAIAIYPMYKGIARLFGFDTPEVTGDF
                     QDEVKLLRDKWFDYDFFFLHYKDTDKAGEDGDFKKKVDRIEYLDQFIPQILALRPDVL
                     AITGDHSTPTLLHSHSWHPSPVLLYSKYAGSSTGIRFTEKECAHGYLGQFPARALMSL
                     MLANALRLGKFGA"
BASE COUNT          787 a          603 c          560 g          780 t
ORIGIN      
        1 gttataaggg ggaggaacat tatggtagtt tttgtggtta attatgaaaa tgagctaaaa
       61 ccttctaata cagattcttt gttcctttcc ttgaaattgg ggaaagaaaa gagtgggaat
      121 agaaatggaa aatagcggaa aagaatggga tttattgaat aaattagacc aagaagaaga
      181 ccttgctcaa gaagagatag ttatacttct tgactgtaca gatagaaaaa tagaagagca
      241 actttttcat cttgctgatg aaaagaggaa aaagtatgtg ggagaagaag ttcaccttcg
      301 aggtttagta gaattttcca atttttgccg tagaaattgc ctctattgtg gattgcgccg
      361 gggtaaccgg aaacttaaaa gatatagaat gtctccggaa gaaatttata accttgctga
      421 aaaaatcgct ctacaggggc tgaaaaccat cgtgcttcaa tcaggagaag atctcactta
      481 tgatgctcgg gctttagctt ctctcattca tcggattaaa aaactcgatg tagcagtaac
      541 tttaagcatc ggagaacgag agccctggga gtatgccttg tggagagatg ccggagcgga
      601 tcgctatctc ctgaaacagg aaacttttga ccgggagctt tttgcaaagc ttcatcctga
      661 tgatgatttc gaccagcgtc tggagtgcca gaaaactctc aaggaattag gataccaggt
      721 gggaagtggg aatatggtag ggcttcccta tcagtctctt catgccttag ctttagatat
      781 taaaaaattt caggaatggg attttgacat gattggtatc ggtcctttta tccccaatcc
      841 tgatactcct ctgggagagg agatttccaa ctctgaggag aaatatattc tcaccttgaa
      901 agttctggct ctcacccgga ttcttacccg aacctctaat cttcccgcta ctactgctct
      961 gggagcactc acctccggag gaagagaaaa agccctctct tgcggagcca atgtttttat
     1021 gcccaatctt actccttctt tttaccagaa aagatacatt atctatcccg ggaaaggcat
     1081 cgaagaatcc tgggaaaatt atcttttaga ggttaaaaaa atggtggaga gagaaggaag
     1141 gatagtatcc accggctacg gagaccgaat ttctctttca ggaagtggcg aggcgatata
     1201 actaattctt tctcgcctcg ccactcaatt ttttcaagct ccaaatttac ccaggcgtag
     1261 ggcgttagca agcatcaagc tcataagagc ccgagccgga aattgcccca gatatccgtg
     1321 agcgcactcc ttttcggtaa aacgtatacc tgtgctactt cctgcgtatt tggaataaag
     1381 gagaactggc gaaggatgcc aggaatgact gtgtaaaaga gtgggggtag agtggtctcc
     1441 agtgatagca agaacatcag gacgcagagc aagaatctgg ggaataaact ggtctaaata
     1501 ttctattcta tccacttttt tcttaaaatc tccatcttct cctgctttat cggtatcttt
     1561 gtaatgcaaa aagaaaaagt cataatcaaa ccacttatcc cggagaagtt ttacctcatc
     1621 ctggaaatct cctgttactt ccggggtgtc aaagccaaaa agccgggcaa tacctttata
     1681 catagggtaa attgcgatag ctaaagcctt cactccatag cgctccggaa atttttccag
     1741 atgcggagat tgagaaaaac cacgcaatag aatggcatta gctttaggtt cttttttgat
     1801 aatctggtta gccttctcta aaaagagctt taaaactcga gcggtttttt ctgcccctgc
     1861 atccagcgcc tggggctcat aaggagggag cccttccttt tgagggtcag tatctctgac
     1921 attttcccct aatccttccc cccgtagaat caaaacaaat ctgtgctcca tacccggttt
     1981 taagattatt tctactcctt caatttcttt tatttcctgg gaaatctttt ctactaaccg
     2041 ggtacattct tcagtagaaa tgcgccctgc tcttctatct actataatgc ctttctcatc
     2101 ccgggtagca aaattagccc gagcagcaat atctccagct tgaagatgga aacccacccc
     2161 cagagcttct aacactcctc gaccgatgat aagctcttca ggattatagc caaataaccc
     2221 gatgtgtcct ggaccacttc ccggggtgat accatagttt accggagtca ttaaccccag
     2281 aacacttttc ttagctaatt catccaggtt gggagttcga gctgcttcca gctctgtttg
     2341 gtaaccaaaa tcaggatggg gaacaccacc taaaccatca gctatgagaa gcactatttt
     2401 gctctctgct tttttgagaa ggctctttaa attttctaac acctcagcca tcttaattct
     2461 ctcctttctc ttattttcct cttatattac cacagttgat tcccaaattc cctatttttt
     2521 acatctttcc tcgttaccta taccagcata aagggggctg ccattcctgg ctcttccaaa
     2581 agaggaaaaa ggaaatacag aaaaagataa attcccgatt atagttaacc gcaaaatcca
     2641 ttacaaatac tataaagggg ctgtgaaggg gctgtgcccc tttataatcc ccctttcttt
     2701 ttccctcgcc tttaatggaa gaaccccaca
//
LOCUS       DAOSVE010000147         2726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_15040_length_2726_cov_88.113066, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000147 DAOSVE010000000
VERSION     DAOSVE010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15040_length_2726_cov_88.113066"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PK016_08475"
     CDS             <1..630
                     /locus_tag="PK016_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HXL03127.1"
                     /translation="KEGLSEVWPILIVGKVGTDLHTTRFDPEVAKTLAKRAAQYGSFI
                     KGHYTDYVENPEAYPLSGMGAANVGPEFTEEEYNALAQLAQLEDKLKEEKLIARPSLI
                     LQVLEKEVYQSKRWQKWLTEEEKGKDLYELPSSRQKWLVKTGCRYIWAQPQVVTSRAL
                     LYHNLEQNGYQGETMVLLQVEKAMDKYFRAFNLINLNDRLRKIIYPVAG"
     gene            1230..1502
                     /locus_tag="PK016_08480"
     CDS             1230..1502
                     /locus_tag="PK016_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002744784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HXL03128.1"
                     /translation="MATQYVLSEYIEQAMAQAIYDKLEDGTFVGRIPSCKGVVAFGAT
                     LRECEESLRSTLEDWILVGLKLKHPLPVIGDIDLNQEPVCEPMDTL"
     gene            1480..1710
                     /locus_tag="PK016_08485"
     CDS             1480..1710
                     /locus_tag="PK016_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006631061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HXL03129.1"
                     /translation="MSRWTPCKRSDFIRRLRQLGFEGPLSGAKHQFMLYEKHRLAIPS
                     YFEYSVPQLRIMIKEVEKIIGRKITADEWNQL"
     gene            complement(1857..2276)
                     /locus_tag="PK016_08490"
     CDS             complement(1857..2276)
                     /locus_tag="PK016_08490"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HXL03130.1"
                     /translation="MIKIQCRGVFLDTSVWIHYFRPQGDEAIKTEVKHALLAESVFTC
                     WVVKTELLVGAKDEDAFDRLSSILEALEEIPLTPEVWLEVARLGYNLRRRGLTVPLPD
                     LLIGQVAAREKLELWHADEHFEYIKEIIPLGTKSFIK"
     gene            complement(2269..2493)
                     /locus_tag="PK016_08495"
     CDS             complement(2269..2493)
                     /locus_tag="PK016_08495"
                     /inference="COORDINATES: protein motif:HMM:NF021474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HXL03131.1"
                     /translation="MSRFTISIDSELLDEAMRLSGKKHKREVIELALGELIKKHQLLE
                     LQSLAGSDLVDWTPEDFASWREAAQRKGND"
BASE COUNT          780 a          574 c          640 g          732 t
ORIGIN      
        1 aaagaagggc tatcagaggt gtggcctata ctcatcgtgg gaaaagtggg aaccgacctc
       61 cacaccactc gctttgaccc cgaagtagcc aagacgctcg ctaaaagagc agcacaatat
      121 ggttctttca ttaaggggca ttacaccgat tatgtggaaa accctgaagc ctatcctctc
      181 tcggggatgg gggcggccaa tgtaggcccg gagttcaccg aagaggagta taatgctctc
      241 gctcaattag ctcagttaga agataaatta aaagaagaaa agctcattgc ccgcccttct
      301 ttgattcttc aggtgttaga aaaagaagtg taccagagta aacgctggca aaagtggcta
      361 acggaagaag aaaaaggtaa agacctgtat gagcttcctt cctcacgcca gaagtggttg
      421 gtaaaaaccg gttgccgcta tatctgggcc caacctcagg tagtaaccag tcgggctctt
      481 ctgtatcaca acttagaaca aaatggttac cagggagaaa caatggtcct tttgcaggtg
      541 gagaaggcta tggataagta tttccgggct tttaatctta taaacttgaa tgaccgcttg
      601 agaaaaataa tctatcctgt agcaggataa aaagtaaaac cactgaggga aagcttttcc
      661 tcagtgctgg ggttttttgg gggattataa aggggtgacg cctcttttcg tcattcccga
      721 gatctttaat cgggaatcca aatgctttta ttctaaataa tatagattcc tgatagaaac
      781 attcaggaat gacaggtaac ggggtgatag aaccattcag gaatgacaga caaagaggaa
      841 agacaccctc accctaaccc tctcccttcg agggagaggg gaaaaagatg gaggggtctc
      901 ccttcaaggg aaaggggaaa tatgtaggtt ttgtcaaggg agagggaaag aaaatcgaga
      961 ttcgctttta tcttttgttt ttccgggggt tatgaggggg cttgccccct catagttctt
     1021 tttactctga cgccgtaggc tgttattttt ttcttttttg ggggtaatag agggtctttt
     1081 tcccctatag tcaggattat aaaggggtac tctctccttt attgtcattg ctacttttca
     1141 aaaaaatatt ttcacttata atagagaagg gaaagtttga acaaaaatag aatgctgaaa
     1201 aggggaaata aagaaacagg aggtgctcgg tggccacaca atatgtctta agtgagtata
     1261 tagagcaagc aatggcgcaa gctatctacg ataaacttga agatggtacc tttgtcggtc
     1321 gaattccctc ttgtaaagga gttgtggcat ttggagccac actgcgagaa tgtgaagaat
     1381 ctttgcgttc tacacttgaa gattggattc tggtaggatt gaaattaaag caccctttgc
     1441 cggtaattgg agatattgat ttaaaccagg agccagtttg tgagccgatg gacaccctgt
     1501 aaaaggagcg attttatccg acggttgcgc cagctgggat ttgaaggtcc tctttccgga
     1561 gctaaacatc aatttatgct ctatgaaaaa caccgcctag caattccctc ctactttgaa
     1621 tattctgtac ctcaacttcg aataatgatt aaggaagtgg agaaaatcat aggtcggaaa
     1681 attaccgctg atgaatggaa ccagctataa taacaccaaa agaggttctc ccccttattg
     1741 ttctttttag tcttacgcca caggctgttc ttcctcgcga tgacgagaag gtgaacaccg
     1801 taggctacca ggcgaagctt gcagtaaccg cccgggcagg taatgatagt gtacatctat
     1861 tttataaaag atttagtccc gagtggtatt atctctttga tatactcaaa gtgttcatca
     1921 gcatgccaga gctctagttt ttcccttgcg gcaacttggc caatcagtaa atctggaaga
     1981 ggaacagtca gccctcgacg acgcaagtta taaccaagtc gcgctacctc taaccatacc
     2041 tctggagtga gagggatttc ctccagagct tcaagaattg agctcaagcg gtcgaaagcg
     2101 tcttcatctt ttgctccgac taaaagctct gttttcacta cccaacaggt aaaaactgac
     2161 tcggccaaaa gagcgtgctt tacttcggtt tttatagctt catctccttg gggtcgaaaa
     2221 taatgaatcc atacagaagt gtccagaaac actccccggc actggatttt aatcatttcc
     2281 tttcctttgg gctgcttctc tccaggaggc aaaatcttcc ggagtccagt ctacgaggtc
     2341 tgacccagcc aggctttgca gctcaaggag ttgatgtttc tttattagtt cccctaaggc
     2401 aagttcaatt acttcccgtt tgtgcttctt gcccgagagc cgcatggcct cgtcgaggag
     2461 ttcggaatca atagatattg tgaatctact catcacacac ctccaaagca tcataatttg
     2521 cactattact atacaccatt tattcgtgta aagcaaatta ccaaattttc tcccgcctgg
     2581 gagggggaag ttgctgagga aaagtatttt ttgaggaaag acaccctcac cctaaccctc
     2641 tcccttcaag ggcgagggga aaaagatgta aagctctccc ttcgagggag aggggaaata
     2701 tgtaggttcc gtcgagggag agggaa
//
LOCUS       DAOSVE010000148         2572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_16274_length_2572_cov_71.900675, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000148 DAOSVE010000000
VERSION     DAOSVE010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16274_length_2572_cov_71.900675"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..1151
                     /locus_tag="PK016_08500"
     CDS             78..1151
                     /locus_tag="PK016_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HXL03132.1"
                     /translation="MLRSLRKHLDVILIIVVIAFAVTIYYGYGSYQRSGTSNQAVAAT
                     VNGTAITFYELDQAFRNLIYQYDSTTLNQMDENSITFLRRLILENMIQNELLYQEAKA
                     RKIKVSSSEIEEQIEAIKAQFPSEEDFRRYLEYQGFNLATLREAIKRELMIQKLEDSL
                     AEEITIPEEEIQKYYEENKSLFITPPQYHLRQMTFSSQEEAEVVLKRLYLGEDFAQLA
                     KDNSKDSYASQGGDLGWVSESSLPPEAKEVLSSLKDKPLAITPVIKVGENYYIYQIIE
                     FKPQEEKNYEEVKEDIEKFLKEEQKRVKIDHLVGELREKSKITIDESLQIAIEAPSPE
                     PGATPMGSPVTTGEENTEETSTP"
     gene            1181..2389
                     /locus_tag="PK016_08505"
     CDS             1181..2389
                     /locus_tag="PK016_08505"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HXL03133.1"
                     /translation="MSHRGRLVSLITGLVLLSLFLSSCSRPIEGNLLGVDLSELKKND
                     LSSFLEIMEERWLESPLILKVNGVTISLNKKELGISWNIEEMKRFILKGKGETVPILF
                     SWEEEKVRSILNDLAQKTYSLPQDARLEGKRVIPSQEGKKLDEEGAIIELREALSRGT
                     AEITLTTLQVIKPDIDTSLILEKLGTSHLLARYQTSLESKDEPTVYNIQKASSSINGY
                     ILEKGEIFSFNNLVGRAEKEDGYLETNIMVNGRLSPGYGGGVCQVSSTLYNALLNTEA
                     RILERHPHSGYSEATSYVPPGRDAAVSYGSKDLRFSFPTQKVVIFAYLQDKNLICEIW
                     GEKGNSLQKTWETRVVSLEKRGEGEGLLTVETTVRNGEKIEYRYQDTYLIPWELGQKI
                     KEDFQNLGNK"
BASE COUNT          880 a          473 c          517 g          702 t
ORIGIN      
        1 agcagttgac agaaaataag ttaatggagt agtattctaa agatgttaaa aacttagaag
       61 tcacagaggt ggattgaatg ttgaggagtt tgcgcaagca tttagatgta atacttataa
      121 tcgtagtaat agcttttgct gtaaccattt attatggtta tggttcttat cagcgctcag
      181 gaacaagcaa tcaagcagta gcagccacgg ttaacggcac ggctatcact ttttatgaac
      241 tggaccaggc ctttcgtaat ctaatttatc agtatgattc tacaactctc aaccagatgg
      301 atgaaaattc tataactttc ttgcgtcgct taatattgga aaacatgatt caaaatgaac
      361 tcctttatca ggaagccaaa gcgaggaaaa ttaaagtttc ttcttctgag atagaggaac
      421 aaatagaagc tatcaaggcc caatttccct ccgaagaaga tttccgaaga tatttagaat
      481 atcagggatt taatttagct acgctccggg aagccataaa aagagagtta atgattcaaa
      541 agctagaaga tagcctagca gaagaaatta ctataccaga agaagagata caaaaatatt
      601 atgaagaaaa taaatctctt ttcattaccc cccctcagta tcacctgcgg cagatgactt
      661 tttcttctca ggaagaggcc gaggtagtat taaaacgctt gtacttaggt gaggattttg
      721 ctcagttagc taaagataat tctaaagatt cctatgcttc tcaaggagga gatttaggat
      781 gggtaagtga aagtagctta cccccagagg cgaaagaggt tctctcctct ttaaaagata
      841 aacctttagc cattactccg gtaattaaag tgggagaaaa ctactatatt tatcaaataa
      901 tagagtttaa acctcaagaa gagaaaaatt atgaagaggt aaaagaagat atagaaaagt
      961 tcttaaaaga agagcaaaaa agagttaaaa tcgaccactt agtaggtgag ttgcgggaga
     1021 aaagtaaaat tactatagat gagtctttac agatagctat agaagcccct tctccagagc
     1081 ctggagctac tcctatgggc tctccagtaa caactggtga agaaaacaca gaggaaacct
     1141 ccactccatg aagtttgaag aaaattatta gaaaataacc atgtctcaca gagggcgttt
     1201 agtttcatta attacaggtt tggtactttt gtctttgttt ctttcttcct gttcccggcc
     1261 cattgaaggt aatttattag gtgttgactt aagcgagctt aaaaaaaacg acctatcctc
     1321 tttcttagag ataatggaag aaaggtggct ggaatcccct cttattctca aagtaaatgg
     1381 tgtaaccatt tctctcaata aaaaggaact gggaatttcc tggaatattg aggagatgaa
     1441 aagatttatt cttaaaggaa aaggagaaac agttcccatt ttattttcct gggaagaaga
     1501 aaaagtcagg agtatcctga atgatttagc tcaaaaaact tattctcttc ctcaggacgc
     1561 ccgcttagag ggaaaaaggg taattccctc ccaggaaggg aaaaaactcg atgaggaagg
     1621 agcaataata gaactccgag aagctctgtc ccgcgggaca gcagaaataa ctcttaccac
     1681 tctccaggtt ataaaaccag acattgatac ctctcttatc cttgaaaaac tggggacttc
     1741 gcatctatta gctcgatacc aaacttctct tgaaagtaag gatgaaccca cagtttacaa
     1801 tattcaaaag gcttcttctt ccatcaacgg ttacatctta gaaaaaggag aaattttctc
     1861 ttttaataat ctagtaggta gagcagaaaa agaggatggc tacctcgaaa ccaatataat
     1921 ggtcaatgga agactatccc ccggttatgg aggaggagta tgccaggtat cttccactct
     1981 gtataatgct ctattaaata ccgaagctcg aattttagag cgtcatcctc attccggata
     2041 ttcagaagct acttcctatg ttcctccagg gagagatgca gcagtgagct atgggagtaa
     2101 agatctgcga ttttcttttc ccacacagaa agtggttatt tttgcctatc tccaggataa
     2161 gaatttaata tgtgagatat ggggagaaaa aggaaactcc ttacaaaaaa cctgggaaac
     2221 ccgggttgtt tctttagaga aacggggaga gggggaaggg ctcttaactg tagaaactac
     2281 agtgagaaat ggagaaaaaa tcgaatatcg ctaccaagat acttatctaa taccttggga
     2341 gctgggacaa aaaatcaaag aagactttca gaatcttggt aataaataac ttctaacata
     2401 gccagaatat tttcacaggg tatatctcga ttaccctttt tcttagccaa ggcaatcaat
     2461 ttctgccatt cgtggtttaa tgaactttct ccaaagagga gaccccataa ttaggcctcc
     2521 ctgccatccc caatcgtcac caaagtgcac tccatcaaaa cccaaattta ga
//
LOCUS       DAOSVE010000149         2540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_16513_length_2540_cov_103.792757, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000149 DAOSVE010000000
VERSION     DAOSVE010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16513_length_2540_cov_103.792757"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1783
                     /gene="glpQ"
                     /locus_tag="PK016_08510"
     CDS             <1..1783
                     /gene="glpQ"
                     /locus_tag="PK016_08510"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: protein motif:HMM:NF008354.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HXL03134.1"
                     /translation="PEFVILVGDNCTGAIGSAEEFNLYVQQMVEPMEKRGIPWAHVYG
                     NHDEDHVEKTGLTKELQQGIYESFPHCLSQRGPEEVEGVGNFVLPVYSSDGSKEVFAI
                     WGLDSNAYLEDNNSWYGGRLENDAILENGLQGSHNYDFIKFSQIRWYWDTSVEMEKKW
                     GYKIPGILFTHIPLPEHRLIAMNPEPTGIIGEKNEDVCNSPINSGLFAAILQRGDIKG
                     IFVGHDHVNDFVGNYCGIKLGYDANIGFGTYGLEGEDKDRLRGARVFRINERTPQALE
                     TWMRYASEFDKNKSSELLSSLLVIAHRGASGYLPEHTLESYAMAYALGADYIEQDVVL
                     TKDKVFICLHDIYLEPTTNVEELYPERKREDGHWYAADFTLEEVKKLRVHERSKADGT
                     PYFPERFPVNYSKFEVPTFEEAIQLIQGLNKSTGKNIGIYPEFKQPSWHEKEKLEGGE
                     KILLDILQKYGYTEKNSGVFVQCFEPEALQKMRFELGTELPLIQLIGSGEEKDAQMLT
                     SEGLEEIASYADGIGILKDYIENNPELVSLMREKGLLVHAWTFRKDAVPEKYPSYEKE
                     LEQLCRECNIDGLFTDFPDIVIKFLER"
     gene            1933..2370
                     /locus_tag="PK016_08515"
     CDS             1933..2370
                     /locus_tag="PK016_08515"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012466090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HXL03135.1"
                     /translation="MKQYQTANLSRKKRIALVAHDHKKPDLLEWAKFNRGLLSEHTLY
                     ATGATGTLLEKELGLQVHKLKSGPWGGDQQIGAKIADGEIDLLIFFWDPLEPLPHDPD
                     VKALLRIAVLWNIPVACNRASADFIISSPLMYEEYERLVPQPV"
BASE COUNT          823 a          482 c          572 g          663 t
ORIGIN      
        1 accggaattt gtgatactgg tgggggataa ttgtactgga gctatcggaa gcgcagaaga
       61 attcaatctt tatgtacagc aaatggtaga acctatggaa aaaagaggaa ttccctgggc
      121 tcatgtttat ggtaatcatg acgaggacca tgtagaaaaa acaggcctta ccaaagagtt
      181 acaacaagga atttacgaat catttcctca ctgtttgagc cagcgtggtc ctgaagaggt
      241 tgaaggagta ggcaactttg ttttgccggt atattcttca gatggcagca aagaagtgtt
      301 tgcaatttgg gggttagatt ccaatgctta ccttgaagat aataattcct ggtatggtgg
      361 caggcttgaa aatgatgcaa ttttagagaa tggattacag ggtagccata actacgattt
      421 cataaagttc tcccagatta gatggtattg ggacacttcg gtagaaatgg agaaaaaatg
      481 gggctataaa atcccgggca ttctctttac tcatatacct ctacccgagc atagactgat
      541 agctatgaat ccagaaccaa ctggtataat aggagagaaa aacgaggatg tttgtaactc
      601 tcccataaac agtggactat ttgctgccat cttacagaga ggagatatca aaggtatttt
      661 tgtaggtcat gaccacgtta atgattttgt gggcaattac tgcggaatca aattaggcta
      721 tgatgccaac ataggattcg gaacctatgg tttagaagga gaagacaaag accgactgag
      781 aggtgccaga gtgttcagaa ttaacgaaag aacccctcaa gccctagaaa cctggatgcg
      841 ctatgccagt gaatttgata aaaataagag ctcagaattg ctttcttccc ttttagtaat
      901 cgctcaccgg ggagccagtg gatatctccc agaacatacc ttagaatctt atgctatggc
      961 ttatgcttta ggagcagact acatagagca agacgtagtc ctgaccaaag acaaagtctt
     1021 tatttgcttg cacgatattt atcttgaacc tactaccaac gttgaggaat tatatccgga
     1081 gcgcaaaaga gaagatggac actggtatgc tgctgatttc actctggaag aggttaaaaa
     1141 actacgagta cacgaacgct cgaaagccga tggcacaccc tattttcctg agcgctttcc
     1201 ggtaaattat tctaaatttg aagtgcctac ctttgaagaa gcaattcaat tgattcaagg
     1261 gcttaacaaa agtacgggta aaaatatagg aatctatcct gaattcaagc aacccagctg
     1321 gcacgaaaaa gaaaaactcg aggggggaga aaaaatcctt ctcgatatac ttcagaaata
     1381 tgggtataca gaaaaaaatt ccggagtttt tgtccagtgt tttgaaccag aagctcttca
     1441 aaaaatgcgc tttgagctgg gaactgagct tcctctaatc cagttaatcg gtagcggaga
     1501 agaaaaagat gcccagatgc taaccagtga aggcttagaa gaaatagcca gctatgccga
     1561 tggcatagga atcttaaaag attatataga aaacaacccg gaactggtta gtttgatgcg
     1621 agaaaaaggg ctattggttc atgcctggac tttcagaaaa gatgctgttc cagaaaaata
     1681 tccttcttat gaaaaggagc tggagcagct ttgccgggaa tgcaatatag atgggttgtt
     1741 tacagatttc cccgatatag taatcaagtt tttagaaaga taagactctt tgctgctctc
     1801 ttttttggga gagagcagca aagtccataa aaatatgaag acctataaat tctggacatg
     1861 atgcaactat aaagttatac tttagtacag ctaataatag aagcaggaaa tgaaagaaag
     1921 gggggtaaat cgatgaaaca ataccaaacg gcaaaccttt ccaggaaaaa aagaatagct
     1981 ctggtagctc acgaccataa aaaaccggat ctcctggagt gggcaaaatt taatcgtgga
     2041 cttctgagtg agcacaccct ttatgccaca ggagctacag gcactctact ggaaaaagag
     2101 ctcggtctcc aggtgcataa acttaagagc ggaccctggg gaggagatca gcaaattggt
     2161 gctaaaattg ccgatggaga aatagacctt ttgattttct tctgggatcc tttagagcct
     2221 ttgcctcatg acccggatgt aaaagcccta ttacggatag cagttctgtg gaatatacct
     2281 gttgcctgta acagagcttc ggcagacttt atcatatcat ctcctcttat gtacgaagaa
     2341 tatgagaggt tagttcctca acctgtgtaa acgaagctgt tgctaatatc gagctgaacc
     2401 agagtctaaa ggaaaaacgt caaatcgctg cagatattat agtatcccac aaaatgtgct
     2461 aaaactttac caccctcact ctctccttcg gagggctttc ctttctatca ttcccgaaat
     2521 ttagccggga atcttcctca
//
LOCUS       DAOSVE010000150         2503 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_16838_length_2503_cov_65.470180, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000150 DAOSVE010000000
VERSION     DAOSVE010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16838_length_2503_cov_65.470180"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PK016_08520"
     CDS             complement(<1..181)
                     /locus_tag="PK016_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL03136.1"
                     /translation="MRIMKTNLESKISALPLESQQALIAHPALFELIKELIEIVERRQ
                     QEIAELRLEVKHLRDQ"
     gene            complement(302..1141)
                     /locus_tag="PK016_08525"
     CDS             complement(302..1141)
                     /locus_tag="PK016_08525"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXL03137.1"
                     /translation="MGWGLAKILAITTLGLWGVEAEQPALLVEEKIEEAEPIEVKVTV
                     IYDNYSLVEGLKTEWGFSCLIEGLEKTILFDTGGRGDLLLENMKKLNINPQEIDIVFI
                     SHDHWDHTGGLATFLQENSEVEVFLLDSFSSTLKDEVTRSGADLREVKGPTVVCSRVC
                     STGTLGTAIKEQSLVIEIEQGLVVITGCAHPGVVDIIRAVKDHYHQDIYLVMGGFHLL
                     DLLLDELQKVTKQFRELGVKMVGPSHCSGDLCRKCFAQEYGKDWVEVGVGRIISFPLK
                     LKS"
     gene            complement(1172..1984)
                     /locus_tag="PK016_08530"
     CDS             complement(1172..1984)
                     /locus_tag="PK016_08530"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXL03138.1"
                     /translation="MIKKKKDFRYIYGPVASWRLGASLGIDLISQKEKVCNFDCVYCQ
                     LGKTLIFPQQRKTYVPTETIIAELKSLPEVQIDYITFSGRGEPTLAENLGQAIKAVKE
                     VRSEPVAVLTNSSLMSYPEVREDLSGADLVVAKLDAYSSASLSKINHPQPGIEFDLVI
                     EGIKQFRVNSNTRLALQIMFIEENKNHPEEFWPLLDYIRPDEVQINTPLRRSPVSPLS
                     PEEIFQIEKCFSTYNEHLPEEGKINLVSVYNAKMKEVAPISSKNTLKRRGKV"
     gene            complement(1959..>2503)
                     /locus_tag="PK016_08535"
     CDS             complement(1959..>2503)
                     /locus_tag="PK016_08535"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HXL03139.1"
                     /translation="ISETKYGPMVHAKLGIAEENSGKLVAMVKQKAQEIAEAKGKAWL
                     IVDGPPGIGCPVIAALARVDLALIVTEPTLSGIHDMERVMDLAAHFGVTAKVVINKCD
                     INWNNTQAIEEICQKREIEVIGRLPFSEEVVRSLILGIPVVEFSQGEIAEEMTALWRK
                     IEAIFQEEGVALGDKKEERL"
BASE COUNT          691 a          625 c          424 g          763 t
ORIGIN      
        1 gctggtctcg aaggtgtttc acctccaaac gaagttcagc tatttcttgt tgccgccttt
       61 caacaatctc tatcaattcc ttgatgagtt caaataaggc agggtgtgct atcagtgcct
      121 gttgagattc taatggtaaa gctgagattt tactttccag attagttttc ataatgcgca
      181 tagtattcct gactccttaa gaactgctaa tggagcttca ataaaaatat atacctctgt
      241 gtagttacct ataatttatc atttttgctt gcgccgcagg ctgtgtgttt acaggctagg
      301 gttaagattt taacttcaaa ggaaagctga taattctacc aactcccacc tctacccaat
      361 cttttccata ctcttgagcg aaacattttc gacaaaggtc gccactacag tgagaggggc
      421 ctaccatttt cacgcctagc tccctaaatt gcttagtaac tttctgtaac tcatctaaca
      481 ggagatcaag caagtgaaag ccacccataa ccaaatagat atcctgatgg tagtggtcct
      541 taaccgctcg aatgatgtcg accactcccg ggtgcgcaca accagttatc accaccaacc
      601 cttgctctat ttcgataacc agtgattgtt ctttaattgc agtccctaaa gttccggtag
      661 aacacaccct cgagcacacc acagttggtc ctttaacctc ccttaaatca gcaccactcc
      721 ttgtaacttc atccttcagg gttgacgaaa aggaatccaa taaaaaaact tcgacctccg
      781 aattttcttg aaggaaagta gctaagcctc cggtgtggtc ccagtggtca tgagagataa
      841 aaactatatc tatctcttga ggattaatat ttagcttttt catattttcc agtaacaggt
      901 cgcctcttcc accagtgtca aaaagaatag ttttctccaa accttcaatc aggcaagaaa
      961 aaccccattc cgttttgaga ccctcaacta gcgaatagtt gtcatagata acagtgactt
     1021 ttacttctat tggttctgcc tcttctatct tttcttcaac taataaagca ggctgttctg
     1081 cctccactcc ccacagtcct aaggtagtta ttgctaaaat tttcgctaaa ccccacccca
     1141 taaaaaatcc ctctctttct cttaattatt tttatacttt ccctcttctt ttgagagtgt
     1201 tcttgctact aataggagca acttccttca ttttggcatt atagaccgaa accagattta
     1261 tttttccctc ctcaggaaga tgctcattat aagtagaaaa acacttttca atctgaaaaa
     1321 tctcctcggg ggatagaggc gacacgggac tacggcgaag gggggtgtta atttgcacct
     1381 catccggccg gatataatca aggaggggcc aaaactcttc cgggtggttc ttgttctctt
     1441 ctataaacat aatctgcaga gccaagcgag tattgctatt taccctaaat tgttttattc
     1501 cctctattac cagatcaaat tctatacccg gttgagggtg gttaattttc gaaagggaag
     1561 ccgaagaata agcatctaac ttggctacta ccagatctgc cccagataaa tcctctctaa
     1621 cttcgggata gctcatcaaa gatgaattgg ttaaaactgc caccggttca gaacgaacct
     1681 cttttactgc tttgatagcc tgacccaaat tttcagctaa agtaggttca ccccgaccag
     1741 aaaaagtaat ataatctatt tgcacctccg gcagagattt aagctctgct attattgtct
     1801 ctgtgggaac ataggttttc cgctgttgag gaaaaatcaa agtcttacct aattggcaat
     1861 aaacacaatc aaaattacat accttctctt tctgagaaat aaggtctatc cccaaagaag
     1921 ctcccagcct ccaagaagct accggaccat agatgtatct aaagtctttc ttctttttta
     1981 tcacccaggg ctaccccctc ctcttggaag atagcttcaa ttttcctcca caatgcagtc
     2041 atttcttcag ctatctctcc ttgcgaaaat tctaccactg gtattcccaa aatcaaagac
     2101 cgcactactt cctccgagaa aggtaagcgt cctatcacct ctatttccct tttttggcaa
     2161 atttcctcta tcgcctgggt attattccaa ttaatatcgc acttatttat aaccaccttg
     2221 gcggttactc caaaatgagc agctaaatcc attactcttt ccatatcgtg gatacctgac
     2281 aaggtaggtt cggtcactat caacgctaaa tctactcgag ctaaggcagc gattaccgga
     2341 caccctattc cgggtggacc atcaacgata agccaggctt ttcccttcgc ttctgctatt
     2401 tcttgagctt tttgtttcac catggcaacc aatttacctg agttttcctc agctattccc
     2461 agcttagcgt gcaccattgg accatactta gtctcagaaa taa
//
LOCUS       DAOSVE010000151         2489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_16969_length_2489_cov_72.506984, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000151 DAOSVE010000000
VERSION     DAOSVE010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16969_length_2489_cov_72.506984"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..823)
                     /locus_tag="PK016_08540"
     CDS             complement(62..823)
                     /locus_tag="PK016_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HXL03140.1"
                     /translation="MSEKYFHPHLTYTDFKEGKFDKAILAVGSAENHGFHLPFGTDTL
                     VSLAIAEGVAKEVEGLLVLPPVTYGVSFHYDDFPFTLTLRPEVLVEVLKDILDSSIRQ
                     GINHIIIINGHDGNIAPIEIASRSIKVKHPDSFIASLDAWWVKAGELLPPQTFEVWGG
                     LGHAGEGETSIVLHLYPQLTQMEKARGVVPDLPDNLDIKWKFSEITPYGATGDPNKGT
                     PEKGEKMFKVLVDCVVKFIQEMEKTGWKYGEKAKL"
     gene            complement(1157..2305)
                     /locus_tag="PK016_08545"
     CDS             complement(1157..2305)
                     /locus_tag="PK016_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXL03141.1"
                     /translation="MGKVFSDLDHKIKVLGSAARFDLCGACGGEESRRRNPQGGWIYP
                     VSLPGSGKRVNILKILLSNNCMHNCFYCANRGGRDHLITSFSAEELSNLFWYLYQKGM
                     VRGIFLSSAVDKSPSWTMSEMLKVAEILRYRFHFSGYLHLKILPDVSNDYLEEAIKLA
                     TRVSVNLEAPNPQSLCEIAPQKNFSRIWQKFQWFRSQKEKGFSTPAGITTQLVVGGSR
                     ETDREIMQTSSQLYRQFGLARVYYSAFQPVADTPLSYKPPVSTWREHRLYQADFLLRQ
                     YKFSFEELIFDEQGNFPLSLDPKTLWANNHPEFFPVEVNTAPWEVLIRVPGIGVTSAR
                     RIMKLRREITFRDSEALSKLGVRVERALPFILMDGKKLKRVEQLSLFT"
BASE COUNT          773 a          552 c          429 g          735 t
ORIGIN      
        1 atagtcatta taattagctt tgcggttaac tataaaacaa cattttacta atgaacactc
       61 tttaaagctt agctttttct ccatatttcc atcctgtttt ttccatttcc tgaatgaatt
      121 tcaccacaca atctaccaac accttaaaca tcttttctcc tttttctgga gtccctttat
      181 tagggtcacc agtagctccg taaggggtga tttcactaaa tttccactta atatccaaat
      241 tatcaggaag atcaggaact accccccgtg ccttttccat ctgagtgagc tgaggataga
      301 gatgtaaaac aatagaagtt tctccctctc ctgcatgccc caaacctccc cacacctcaa
      361 aagtttgagg agggagtaat tctccagctt tcacccacca ggcgtctaaa gaggcaataa
      421 aagaatcggg atgtttcact ttaatcgagc gagatgctat ttcaataggg gcaatattac
      481 catcgtgtcc attgatgata ataatatggt taatcccttg tcttatagaa gaatccagaa
      541 tatcttttaa tacttctacc aaaacttcag gtcgaagagt caaagtaaaa ggaaagtcat
      601 cataatggaa actcacccca taagttacag gaggcaaaac caagagtcct tctacttctt
      661 tagctactcc ttctgcaata gccaaggaaa ctaaagtatc agtaccaaaa ggcaggtgaa
      721 aaccatgatt ttctgccgac cctactgcca gaatagcctt atcaaattta ccttctttaa
      781 aatcggtata ggtaagatga ggatgaaaat acttttcgct catttttatt cctcctttta
      841 agggattcag caacttacac atattacctt tcaggtagca aaatagtccg gcctaaatat
      901 ctgaaaagtt tggtataaat actttaacaa gcctcattct gccagattat aactgaccgc
      961 aaaatttatt ataacattcc cctacttagc gctttgttta tctatgtatt gatattattg
     1021 cgacaactcc ccctcgtcaa tcgcgagcct caatttttcg catggtgtgg cgatcccaag
     1081 tttttaaccc aatgtaactt gcctttaaag tttgagcaat ttaaaaggtt ttgcggaatg
     1141 ccatattatc aaaaaatcag gtaaaaagag atagttgctc cactcttttt aactttttac
     1201 catccataag aataaatgga agagctctct ctacccgtac tcctaattta gagagtgctt
     1261 cggagtcacg aaaagttatc tcccgacgca atttcataat tcgtcgtgct gaagttaccc
     1321 ctataccagg aaccctgatt aaaacctccc aaggagcagt atttacttcc acggggaaaa
     1381 attcagggtg gttgttagcc cacaaagttt tagggtcaag agacaaaggg aagttaccct
     1441 gttcatcaaa aatcagctct tcaaaagaaa atttatactg acggagcaaa aaatcagctt
     1501 gatatagacg atgttctcgc caggtagata ccggaggttt ataagaaaga ggagtgtcgg
     1561 caactggctg aaaagcactg tagtaaactc gagctaaacc aaattgacgg taaagttgag
     1621 aagaggtttg catgatttct ctatcggttt ccctgcttcc tcctactacc aactgggtgg
     1681 taatccctgc tggggtagaa aagccctttt ctttttggct cctgaaccat tgaaatttct
     1741 gccagatacg agaaaaattc ttttgaggag caatttcaca gaggctttga ggattaggag
     1801 cttctaagtt aactgatacc cgggtagcca gcttgatagc ttcttctaag taatcattgc
     1861 ttacatcagg taatattttg agatgaagat aaccagaaaa atggaaacga tagcgaagaa
     1921 tttctgctac tttcagcatt tcgctcatag tccaggaagg gcttttatct acggcagagc
     1981 ttaaaaaaat acctctcacc atacctttct ggtaaagata ccagaaaaga ttagatagtt
     2041 cttcagcaga aaaagaagta ataagatggt ctctacctcc ccgattagca caataaaagc
     2101 aattatgcat gcaattatta gaaaggagga ttttaagaat atttactctt ttcccgctac
     2161 cgggaagact aactggataa atccaacctc cctgaggatt acgcctcctc gattcctctc
     2221 ctccacatgc tccacacaaa tcaaaacggg ctgcacttcc taaaactttt attttgtggt
     2281 ctaaatctga aaagacctta cccattttta tcccctgtct attttactcc atttaaccca
     2341 ttttagccat tcttagagag aaatcacgct acgcctccct aggcatcatc tgactcagga
     2401 atattaatga cctcctcaat acttaagcct ctaacatgct cggtcttcac ccagtgtttc
     2461 tggtttccgg acaaaaatag agagtaaaa
//
LOCUS       DAOSVE010000152         2487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_16993_length_2487_cov_75.372122, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000152 DAOSVE010000000
VERSION     DAOSVE010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16993_length_2487_cov_75.372122"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..1708
                     /locus_tag="PK016_08550"
     CDS             245..1708
                     /locus_tag="PK016_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HXL03142.1"
                     /translation="MKKQEPLFLGIDLGTQGVRCLVSTPQGEIVAEGKEKISHIYREG
                     EQFEQEPEEWWEVTTKALREALRNLREKGKDPNSIQALSCDSTSGTVLALNREGQALS
                     RAIMYNDGRAKEEAEIINREATSFTERLGYQFGASFALAKILWWKRNNPELFDKAHLF
                     LHAADFIVGKLTGEWGISDTSNSLKMGYDLIDFKWPSFIETQLGISISKLPQVVNPGE
                     VVGKIRPQVAQELSLSPHCKVVAGCTDGTASFLASGATRPGDTSCALGTTLVVRSISY
                     RLIKDPEGRIYCHRHPEGYWLPGGASNTGGECLEVYFPGEDYQKWDEKISQDNLPTSI
                     LLYPLTRRGERLPFSSGEACFFQIGKEKNREEFYAACLEGVGYIERLSLELLENLGAD
                     PITRVFASGGGSRSSIWRKIRANILNRPIFLAQTTESAFGSCILSASLFWGKISRAVQ
                     EMVHLSGVEKPHPLLSSLYEERYQAFLMECKKRGYIS"
     gene            complement(1807..2292)
                     /locus_tag="PK016_08555"
     CDS             complement(1807..2292)
                     /locus_tag="PK016_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL03143.1"
                     /translation="MAKPWSEEEDEIILNNPKLNSQELSELLNRSQKAIMHRRARLRG
                     KDNRGNNNILITSDWHIPHYDKEILELLLNTAKENNTQELIIAGDFLNLDILSTFAKR
                     ETPISVDEELKEAKEAVLYADDYYPESSGVDKLKEAIEYFKAAAQKAGLWVSDGKQGR
                     K"
BASE COUNT          685 a          496 c          567 g          739 t
ORIGIN      
        1 agggaaagag taacatccta taatatcgct gaggagagtt tttccttcct cagtagtctt
       61 tttctttttg tatttttttg tttttggggg attataaagg ggttctcccc tttatagttc
      121 ggggttatga gggggtcttt ttccccctca tagtcattat aatggatttt gcgattaacc
      181 ataatagata aggcaaataa attataatac aatcacaaag acgcaagaaa ggaaaactgc
      241 taagatgaaa aagcaagaac cactattctt gggaattgac ttaggaactc aaggggttag
      301 atgtttagtc tctacccctc agggggaaat agtagcagaa ggaaaagaaa agatttctca
      361 tatttaccgg gagggagagc aatttgagca agaaccagaa gaatggtggg aagtaactac
      421 taaagccttg agggaagctc tccgcaacct gagagaaaaa gggaaagacc cgaattctat
      481 tcaggcttta tcttgtgatt ctacctccgg tactgtttta gctctcaacc gagagggcca
      541 agctctcagc cgggctatta tgtacaatga cggaagagcc aaagaagaag cagaaattat
      601 aaaccgtgaa gctacctcgt ttaccgagcg attaggttac caatttggag cttcttttgc
      661 tttagccaaa atattgtggt ggaagagaaa taatccagaa ttgtttgata aggctcatct
      721 ttttctccat gctgctgact ttatagtggg aaaattaact ggagagtggg ggatatcgga
      781 tacctccaat tccttgaaga tgggatatga ccttatcgat tttaaatggc cctcctttat
      841 cgaaacccaa ctgggcattt ccatctccaa actccctcaa gtagtcaatc ctggagaagt
      901 agtaggaaaa ataagacctc aggtagctca agaactgagt ttatctcccc attgtaaggt
      961 agtagcagga tgtactgatg gcacggcatc ttttttagct tccggagcca cccggccagg
     1021 tgacacatcc tgtgctttag gaactactct tgtggtcaga agtatcagtt atcgactgat
     1081 taaagacccg gagggacgga tttactgcca tcgccaccct gaagggtatt ggttaccggg
     1141 aggagcgagt aacaccggag gagagtgttt agaggtatat tttccaggag aggattacca
     1201 gaagtgggac gaaaaaataa gtcaggataa tcttcctacc tctattctgc tttaccccct
     1261 cacccggaga ggagaacgtt tacctttttc ctccggggag gcttgttttt ttcagatagg
     1321 taaagaaaaa aaccgggaag aattttatgc tgcctgttta gagggagtgg gttatataga
     1381 gcgactgagt ctggaacttt tagaaaacct gggagcagac cctataactc gagtgtttgc
     1441 ctcaggaggt ggttctcgta gttccatctg gcgaaaaatc cgggctaaca ttttaaaccg
     1501 ccctattttc ctggctcaaa ctaccgaatc agcttttggc tcttgcattt tgtcagcttc
     1561 ccttttttgg ggaaagatat cccgagcagt tcaagaaatg gttcacctct ccggtgtgga
     1621 aaagcctcat cctcttcttt cttctctcta tgaggaacgt tatcaagctt tcctgatgga
     1681 atgcaaaaaa cgaggttata tttcttagcc cgggggtggc acttatagca tcacccttat
     1741 ccctcccgca aggaacgata aagcagtagc accataagag aaaataccgc gcttgataag
     1801 ctactcctac tttcttccct gcttgccatc actcacccac agtcctgctt tctgtgctgc
     1861 tgctttaaag tattctatag cttccttgag cttatccacc ccggagctct cggggtagta
     1921 atcatcagca tataaaacag cttcctttgc ttctttgagc tcctcatcaa cgcttatcgg
     1981 tgtctctctt ttggcaaagg tagataagat atctaagttt aagaaatcac cagcaatgat
     2041 gagctcttga gtgttatttt ctttggcggt atttaataaa agctccagta tctctttatc
     2101 ataatgggga atgtgccagt cagaagtgat aaggatatta ttgtttcctc tattatcctt
     2161 accccggagt cttgctctcc ggtgcataat tgctttttgt gagcgattaa ggagctctga
     2221 gagctcttga gagttgagtt ttgggttatt gaggattatt tcgtcttctt cttcgctcca
     2281 cggtttcgcc aattcctaac ctccttatga ataaaaaaga ttaggtgact aaaaagctag
     2341 ttagctttta ttctactgga ttgtaagttg gtaaagtagt aaaattgggg aaagtatatc
     2401 ctaaaggggg gtaaagaagt atgaaaataa gaaacctcgg ttatctggga ttgttgggtt
     2461 tattgggttt gctgggtctt atcacgg
//
LOCUS       DAOSVE010000153         2432 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_17528_length_2432_cov_68.205301, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000153 DAOSVE010000000
VERSION     DAOSVE010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17528_length_2432_cov_68.205301"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..1135
                     /locus_tag="PK016_08560"
     CDS             119..1135
                     /locus_tag="PK016_08560"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HXL03144.1"
                     /translation="MILLFSEEGGWKLDKKPRVLVTRRIPQEGLNILEESCQVEVSEY
                     DGVLPREILLQKVKGVDGILCLLTDNIDRKVMEAAGQSLRVISNYAVGYDNIDIEEAT
                     RRRIMVTNTPGVLTETTADLTWALLMAVARRIAEGDRFVREGKFREWEPLLFLGDDVY
                     GATLGIIGLGRIGQAVARRARGFNMQVIYFSHHRLEDKEKELGVTYYPFLELLAQSDF
                     ITLHVPLTRETYHLIGEEELKMMKKESYLINTSRGSVIDEKALIRALKEGWIRGAALD
                     VFEQEPQVEPELLELEEVVLTPHLGSASRTTRTKMAILAANNLALALRGEIPPYLVNL
                     DVVK"
     gene            1254..2096
                     /locus_tag="PK016_08565"
     CDS             1254..2096
                     /locus_tag="PK016_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HXL03145.1"
                     /translation="MGKVSLQNLLKKVDDLPALPSVVKRVMELTEDPNSTARDINEVI
                     NQDQSMTAKVLRLANSAFYGFPRRISTVTDATIMLGFQAIRSIVIAASVSKLLSQEVE
                     GYALLPGELWRHSQSAAIAARMIARKVKFPKTEVAYTAALLHDIGKVVLNSYVRETYE
                     EVMDKVEKEKIPFSQAEEEILGFNHALVGSKVAENWNLPPELVEAIAFHHSPELAKTN
                     PVLTSIVHVADFICVTMGIGIGADGLLYPLSPPAVNLLGLGEQDIYQIISQLSDLLAD
                     QDSF"
BASE COUNT          699 a          441 c          560 g          732 t
ORIGIN      
        1 gctactatag aattttatag agaagctcaa gaaggcttcc atctttatag gacaagcgag
       61 cttatttctg gaatttctta agggaaaaca cgaaaagtag tataatgaaa tagacattat
      121 gattctgttg ttttctgaag aaggagggtg gaaattggat aaaaagcctc gagttctggt
      181 gactcgccga attccccagg aagggctgaa catcttagag gagagttgcc aggtagaggt
      241 aagcgaatat gacggagtcc ttccccgaga gattctcctt caaaaggtaa aaggggtaga
      301 tggtattctg tgtttactga ccgataatat cgaccgcaaa gttatggaag cggcagggca
      361 gagtttgcgg gtgatttcta attatgccgt ggggtatgat aatattgata tagaagaagc
      421 cacaaggagg agaataatgg ttactaacac ccctggagtt ctcactgaga ccactgctga
      481 ccttacctgg gctctgctta tggccgtagc tcgtcgaata gcggagggag acaggtttgt
      541 tcgggaaggg aaatttagag aatgggaacc acttcttttt ctgggagatg atgtttatgg
      601 ggctacttta ggaattatag gtttgggtcg gattggtcaa gccgttgccc gcagagctcg
      661 agggttcaat atgcaggtaa tttactttag ccatcaccga ttggaagata aggaaaaaga
      721 attaggagta acttactatc cttttcttga gcttttggct cagtctgatt ttatcactct
      781 tcatgtgccc cttactcggg aaacttatca tcttattggg gaagaggaac ttaagatgat
      841 gaagaaggaa tcttacctta taaatacttc tcgagggtct gtaatagatg agaaagccct
      901 aatccgggct ttgaaggaag gatggattcg cggtgcagct ctcgatgttt ttgagcagga
      961 gccgcaagta gaaccagaac ttttggaatt ggaggaagtg gtattaacgc cgcatcttgg
     1021 ttcagcttct cgtactaccc ggactaaaat ggcaattctg gcggcaaaca acctggcttt
     1081 ggcactccgg ggagaaattc ctccttatct tgttaatctg gatgtagtca aatgatttgt
     1141 ttaatgatta acaaaaagct aatacttggc aaagaaattt ctctatcttt tgaaaagatg
     1201 agaaatattt tcaaaaaatt taactatttt tagggtcgaa aggaaaaaga aaaatgggta
     1261 aagtttctct tcaaaatttg ctcaaaaaag ttgatgacct tcctgccctg ccttcggtgg
     1321 taaaaagggt tatggagctt actgaagacc ccaattctac tgctcgagat atcaatgaag
     1381 taataaatca agaccagagt atgacggcta aagttttgag gttagccaac tcagcttttt
     1441 atggttttcc ccgtcgcatt tctactgtta ccgatgccac cattatgctt ggtttccaag
     1501 ccattcgctc tattgttata gctgcttcag tgagtaaact tctcagtcag gaggtagaag
     1561 gttatgcact tttacctgga gaactgtggc gacactctca atcagcagct atagctgcta
     1621 gaatgattgc gcgaaaggtg aagtttccta aaacagaggt ggcttatact gcagcactgc
     1681 tccatgatat tggcaaagta gttcttaata gttatgtgcg agaaacttac gaggaagtga
     1741 tggacaaggt agagaaagag aagattcctt tttctcaagc agaagaagaa atattaggct
     1801 tcaatcatgc tctggtaggt tctaaagtag cagaaaactg gaatcttccc cctgaattgg
     1861 tagaagctat cgctttccat cacagcccgg agctggctaa aactaatcct gtgcttacct
     1921 ctatagttca tgtggctgat tttatatgtg tgacgatggg tattggtatt ggagcagatg
     1981 gtttgttata tcctctttct cccccagctg taaatttact tggtttgggg gaacaggata
     2041 tctaccagat tatttctcaa ctgtctgatc tcctggcaga ccaggatagt ttttagtcct
     2101 cagcagtttt tcttaaaaac ggaggaaata taaactaaaa aggtaagatt catattgctg
     2161 accttcagac acagtttcga gaaatcttct gtatgatttt taaatgcttc tcaggccctt
     2221 tttaatagct ataaagcctg tcaaattctc tccgctcgta gtcttagtaa gatgaagagt
     2281 ccagaataaa ggttcttttt tgcgtgtact tctttccata gtgcctcatc acctaaccca
     2341 acttattgat cagtgatagt cttgatttaa cacaagacaa ataatgggtt aagccttttc
     2401 tatgatattg ttctactctt ctgaactgat tt
//
LOCUS       DAOSVE010000154         2359 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_18277_length_2359_cov_66.211806, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000154 DAOSVE010000000
VERSION     DAOSVE010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18277_length_2359_cov_66.211806"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PK016_08570"
     CDS             complement(<1..237)
                     /locus_tag="PK016_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL03146.1"
                     /translation="MNIFFTTFLLILVLPAFSSEIVGFFSLLDNMDRFLSSEVTPQRL
                     EEFNHKKVIPVFQLEADDAKALNEPVQELLNIEKE"
     gene            690..2246
                     /locus_tag="PK016_08575"
     CDS             690..2246
                     /locus_tag="PK016_08575"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL03147.1"
                     /translation="MKRTGLLNRGKRLAHFYLFHYLRFYSRRFRPQDNIRREEIEKGL
                     QAVVKDGLASQAMSTLTGGAFLVDFALLLGASNFFIGVLAAIPALTQLIQIPATFLVE
                     KTRRRKLIVAGGAFASRIFLLLFALIPFLFSPPRRITALSLTILFHAACNSLVSCSWS
                     SWMRDFIPENILGSFFSRRMALSYSLGATLGLAGAFYLDFWKRSFSNPLTGYSLLFLG
                     GVVAGFFSFYYLTLPPEPRSGGEGKAIGFAQLFLSPLKDQNFRNLLFFSGSWHFAYNL
                     ATPFFTVYLLRRLEYNMITVIILSTISQVMSILFFRWWGSIADHFSNKSVLLVSSPIF
                     LASLFLWTFTTLPEKHTFTLPLLVIIHILLGVSTAGILLATGNIGMKLAPRGQATPYL
                     ATYNLVNSLSLGIAPLLGGKLADFFAQRELSLSLRWISPAGERYFETLSFEHWDFLFF
                     FSLLIGIYALHRLTFVKEVGEKKVAIGELATEIGRTVHNISSAGGIFLIETFRFPLRR
                     KEKKKKIYLY"
BASE COUNT          620 a          514 c          445 g          780 t
ORIGIN      
        1 ttccttttca atattcagaa gctcctgtac aggttcattg agggctttag cgtcatcagc
       61 ttctagctga aacacgggaa tgactttctt atgattaaac tcttcgagac gctgaggagt
      121 gacttctgaa gaaaggaagc ggtccatatt atcgagaagg ctaaaaaaac caactatttc
      181 agatgaaaaa gcaggaagaa ccagtataag taaaaatgtt gtaaagaaaa tgttcaaaaa
      241 acagaatcgt ctcattaggc taacctacaa actatattga ttttcttcat tgtataaaag
      301 ctttcttgct atgtcaatgc gccgagaggc tgtttaaaaa atccctaata tttttgggga
      361 atacaatttc tttatatact cagaagtatg tttacccttc caggctctga gctacccttt
      421 tgtaactctt cttttgtcaa tcgtgagcct cacatttccg tatgacgtgg caatcccgag
      481 tttctagccc aatttaattt accttctagt atttattaaa gattcagaaa cagatcgcca
      541 caaccggcaa aaaccgccgt gctcgcgatg acggttcaat taccatgggt tttgcggtca
      601 actatatttc ccctctataa taaagaggaa ctatctttta agaaagaccg taatattaag
      661 caggaatttc tctcggagag gtaaaaagat tgaagaggac tggactatta aaccgaggaa
      721 aaagattagc tcatttttat ctgtttcatt atctccgttt ttattctcgg aggtttcgtc
      781 cccaggataa tattagaaga gaagaaatcg aaaaaggttt acaggcagta gtcaaagatg
      841 gcttagccag tcaagctatg tccactttga ccgggggagc atttctggta gactttgccc
      901 tgcttctcgg ggcttccaat ttcttcatcg gagttttagc tgctattccc gcccttaccc
      961 agttgataca gattcctgct acttttttag tagaaaaaac ccgcagaaga aagctgattg
     1021 tagcaggagg agcatttgcc agtcgtatat ttcttttgct tttcgccctc attccttttt
     1081 tattttctcc tccccggagg attacagctc tatctctcac tattctcttt catgcagctt
     1141 gtaactctct ggtttcctgt agctggtctt cctggatgcg agattttatt cctgaaaata
     1201 ttttagggag tttcttctcc cgccgtatgg ctctttctta ttctttagga gcaaccctgg
     1261 gccttgcagg agctttttat ctggatttct ggaaacgttc tttttctaac cctctcactg
     1321 gctattccct tctcttttta ggaggagtgg tagcaggctt tttcagtttt tactatctaa
     1381 ctcttcctcc tgaacctcgc tcaggaggag aaggaaaagc gattggtttt gctcagctat
     1441 ttctttctcc tcttaaagac cagaattttc gtaatttgct ctttttctct ggctcctggc
     1501 attttgctta taacttagcc actccttttt tcaccgttta tctcttgcgg cgcttagaat
     1561 ataatatgat tacagttatc atcttatcca ccataagcca ggttatgagc atcttattct
     1621 tccggtggtg gggtagtatt gctgaccatt tctccaacaa atctgttctt ttagttagca
     1681 gtcccatatt tctggcttct ctatttttgt ggactttcac tactctacca gaaaaacata
     1741 ctttcaccct gccgttgctc gtcatcatcc acattttgct gggagtttcc actgccggca
     1801 tcctcttagc taccggaaac ataggtatga aattagctcc tcggggacaa gccaccccgt
     1861 atctagccac ttataacctg gttaactctc tatcactggg catcgctcct cttttgggag
     1921 gaaagttagc tgactttttt gcccagcgag agctatcttt atccctccgc tggataagcc
     1981 ctgcaggcga gcgctatttc gaaaccctct cctttgaaca ctgggatttt ctgttcttct
     2041 tctctctctt aatcgggata tacgctcttc atcgtttgac ttttgttaaa gaagtaggag
     2101 aaaaaaaggt ggccatagga gaactggcaa ccgaaatagg aagaacagtt cataacattt
     2161 cttcagctgg ggggattttt ctaatagaaa cctttcgttt tcctctccgc agaaaagaaa
     2221 aaaagaaaaa gatatatctt tattaaaaaa ttttaaactt accttaatcc tcgcttaaac
     2281 ctccggtgtt ttaatgagaa caaatcttaa aaagactgga ggtagtagaa tgaaaagaaa
     2341 ttggtggatt ttgccttta
//
LOCUS       DAOSVE010000155         2334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_18547_length_2334_cov_60.329969, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000155 DAOSVE010000000
VERSION     DAOSVE010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18547_length_2334_cov_60.329969"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="PK016_08580"
     CDS             <1..989
                     /locus_tag="PK016_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXL03148.1"
                     /translation="ADGKWKFFFVERLYRLAYREIEEDGKVSQKMVIVNTQTNEPLIE
                     DKGSFYDIDENGNQVFLIGYSTWTGLENFLRIFNDPRISGPFMSIFVWTIMWSLFSVL
                     FSFLLGLAFASVLNNEKLRGRNIYRTLLIIPWAVPYFISVLTWKNGIFNETYGILNKI
                     ILPFLGLDSIKWFNDQYWAKLACIIVNTWLTFPYMMTISLGALQSIPDELYEVAAMDG
                     AGKWHNFRYITFPLLLTVVAPLLISSFAFTFNNFTLIYLLNSGGPPMVGATTPAGHTD
                     ILISYVYKLAFEGRGQEFGFASAISVIIFVLVGGISLLNFKISGAFEEIRNE"
     gene            993..>2334
                     /locus_tag="PK016_08585"
     CDS             993..>2334
                     /locus_tag="PK016_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL03149.1"
                     /translation="MIERGRPLLTHIILWILIPVILFPIIWIVSTSIRRDEAVFSTKL
                     FSSRVSLQNYRDLIAPEKNMPALVQEMQNLILRASPYDSWKEERLKEAVLEDIKAFKG
                     YLQETRDRYERAQSSFDNINLALSSRSKNIKEEVLSSLRELKEYLEKNISSQGLNEES
                     SKVVLYQLLYPESYNSKLFTYLKEDLEKVSGYHINSPEDYEKAFSVLEKTYQMSLGSL
                     KTELAQLEEKLEENSRNYSMVRAEYEKIEEEILSINQILNSEVQLETTSILEELKAAE
                     TTRGAEKKLVSLSKDASLSASVPQPLKTLPLVIENLSKYPDFYELSDDWREISLYLTN
                     YEETAEGNLASLYPILEDLYIKILSLLSGLEKNINDLQETEKTLIAFKEENTLLKNQQ
                     ELLGEEIKKVEETLILATKAEQVQIFIKKIEEQIADIEEATNFEELINYCASIAK"
BASE COUNT          759 a          467 c          458 g          650 t
ORIGIN      
        1 ctgcagacgg taagtggaag tttttctttg tggagaggct ttaccggctg gcataccgag
       61 aaatagaaga ggatggaaaa gttagccaaa aaatggtaat cgtgaatact caaaccaacg
      121 aaccccttat tgaagacaaa ggttccttct atgatataga tgaaaatggc aaccaggtat
      181 ttttaatcgg atacagtacc tggacaggac tggaaaattt ccttcgaata tttaacgacc
      241 caaggatttc tggtcctttc atgagtatat ttgtctggac tattatgtgg tctttattca
      301 gcgtgctttt ctcctttctt ctgggattag catttgcctc agtgttaaac aatgaaaaat
      361 taagaggaag aaacatttat cgaactcttc ttataattcc ctgggctgtt ccctatttta
      421 tctccgtttt aacctggaaa aatggaatat tcaatgaaac atatggaata ctcaataaaa
      481 taatactccc ttttttaggt cttgactcca ttaaatggtt taatgaccag tactgggcaa
      541 aattggcatg catcattgta aatacctggc tcactttccc atatatgatg accatttctt
      601 taggggcttt gcaatccatc cctgatgaac tttacgaagt ggcagctatg gacggagctg
      661 gaaaatggca taatttccga tacataactt tcccgctact tttgacagtt gtggcaccct
      721 tgctcataag cagctttgct tttaccttta acaactttac tctcatatat ttactgaatt
      781 caggtggccc tcctatggtt ggcgccacca cccctgccgg tcacactgac attttaattt
      841 cctacgtgta taagctggcc tttgaaggaa gaggacagga atttggcttt gccagcgcta
      901 tttctgtcat tatattcgta ctggtaggag gcataagcct cctgaatttc aagatttcgg
      961 gagctttcga agagataagg aatgaataga caatgataga aaggggaaga cctcttttaa
     1021 ctcatataat tctctggatt ctcataccgg tgattctttt ccccatcatc tggatagtct
     1081 ctacttctat aagaagagat gaggctgttt tttccaccaa acttttctcc tcccgagtga
     1141 gcttgcagaa ctaccgagac ctgatagctc cagaaaagaa catgcctgct ctggtacaag
     1201 aaatgcagaa tctgatttta agggcttctc catacgatag ctggaaagag gaaaggctaa
     1261 aagaggctgt gcttgaagat ataaaagctt tcaagggtta tttgcaagaa acccgggata
     1321 gatatgaaag agctcaaagt tcttttgata acataaattt agcgctttct tcccgcagca
     1381 agaacataaa agaagaggtt ctttcatccc ttcgagagtt aaaagaatac ctggagaaaa
     1441 acatttcctc tcagggatta aacgaagaaa gctcaaaagt ggtgctttat caacttctct
     1501 accctgaaag ttataactca aaattattca cttatctaaa agaagacctg gaaaaagttt
     1561 ccggttacca tattaactca cccgaggatt atgaaaaagc cttctctgtg ctggaaaaaa
     1621 cttaccagat gtctctgggc agcctaaaaa ctgaattagc tcaattagaa gaaaagttag
     1681 aggaaaattc cagaaactac agcatggtaa gagcagagta tgagaagata gaggaagaaa
     1741 tcctctctat aaaccagatt ctaaattcag aggtccaact ggagacaact tccattttag
     1801 aagaattgaa agctgcagaa accacgaggg gtgcagaaaa gaaactcgta tccctctcca
     1861 aagacgcctc tcttagcgct tctgtccctc agccattaaa aacattacct ctagtgatag
     1921 aaaatttgag caaatatcct gacttttatg agctaagtga tgattggaga gaaatttccc
     1981 tttatcttac caactatgaa gaaaccgcag agggcaattt agcttcacta tacccgattc
     2041 tggaggattt atacataaaa atactctctc tcctcagcgg actggaaaaa aatataaacg
     2101 accttcaaga aacagaaaaa accctcattg ctttcaagga ggaaaatact ctccttaaaa
     2161 accagcagga gctcctcggg gaggagataa aaaaagtaga agaaactctt attttggcta
     2221 ccaaagctga gcaggtgcag atattcatca aaaagataga ggaacaaata gcagatatag
     2281 aagaagctac aaacttcgag gagctcataa attactgtgc atccatcgcc aagg
//
LOCUS       DAOSVE010000156         2313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_18764_length_2313_cov_72.439770, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000156 DAOSVE010000000
VERSION     DAOSVE010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18764_length_2313_cov_72.439770"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..1938)
                     /gene="thrS"
                     /locus_tag="PK016_08590"
     CDS             complement(118..1938)
                     /gene="thrS"
                     /locus_tag="PK016_08590"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HXL03150.1"
                     /translation="MNVNSREAVAAVAEDGRLVDIGEEEKIKKYLSFEDEAGREVYWH
                     STSHIMAQAVKRLFPEAKLAIGPAIEEGFYYDFDLPRSLTDEDLEKIEEEMRKIIQAD
                     LPFKRREISREEAQKIFAERGETYKLEILKEIEEDTVSIYEEGEFLDLCRGPHVPSTG
                     YIKAFKLLSVSGAYWRGKEDNPMLQRIYGISFDSEEKLQAFLTQREEAKKRDHRRLGK
                     ELDLFSLHEEGPGFPFFHPKGMVVINTLLDLWRREHLRRGYQEVKTPMILDRALWEQS
                     GHWEHYRDNMYFTKIDDRDFAIKPMNCPGGILIFKSQLHSYRELPLRWAELGLVHRHE
                     LSGVLHGLMRVRCFTQDDAHIFMETHQIKQEIIGVIDLADYFYRLFGFSYEIELSTRP
                     ENSMGSDEMWELATRSLEEALKELKIDYRINPGEGAFYGPKIDFHLRDCLGRRWQCGT
                     IQLDFAMPEKFDLYYIGEDGDRHRPVMLHRTVLGSVERFLGILIEQFAGAFPLWLAPV
                     QVVILPIAERHLDYAREIKDLLFSEEIRVEVNDENATLGAKIRKAEMEKVPYLAIVGD
                     REMENRTLSIRKRKKGDLGSFALDELLKNLKKEIEEKVID"
BASE COUNT          648 a          563 c          378 g          724 t
ORIGIN      
        1 tctatttcta accataccag atccgtgtgg attctttttt cccagttttt atcccgcttt
       61 ttttctcttc tcactcctcc attccaaaaa aggagatgag aagtttctct ccccactcta
      121 atctattacc ttttcctcta tctctttttt aagatttttc aataattcat ccagcgcgaa
      181 agaacccagg tctcccttct tacgttttcg gatggagagt gtccgatttt ccatttcccg
      241 gtctcccacg atagcaagat agggaacttt ttccatctcc gcttttctga ttttagctcc
      301 caaagtggca ttttcgtcat taacttctac tcttatttct tcactgaaaa ggaggtcttt
      361 tatttcccgg gcataatcaa gatgtctttc agcaataggc aaaattacca cttgcactgg
      421 tgctaaccac agagggaaag ctccggcaaa ttgctcgatt aaaataccta aaaaccgttc
      481 aacacttcct aaaaccgttc ggtgaagcat cactggccgg tggcgatctc catcttcgcc
      541 aatgtagtaa aggtcaaatt tttcaggcat agcaaaatct aattgaatag ttccacactg
      601 ccatcttctc cccaagcaat cacggagatg aaaatcaatt ttcggcccgt aaaaagctcc
      661 ctctcccggg ttaattcgat agtcgatttt cagctccttc aaagcttcct ctagcgaccg
      721 tgtggccagc tcccacattt catcagagcc catagaattt tcgggacgag tgctcaattc
      781 tatttcgtag ctaaagccaa acaaacggta aaaataatca gcaaggtcga tgacccctat
      841 aatttcctgt tttatctgat gagtctccat aaaaatatgg gcatcatctt gagtaaaaca
      901 acgaaccctc attaacccat gcaaaacccc ggaaagttca tggcgatgaa ctaaccccaa
      961 ttctgcccac cggagaggta gttctcgata actatgaagc tggctcttaa aaatcaaaat
     1021 cccacccgga cagttcatgg gctttatggc aaaatcccgg tcatcaattt tagtaaaata
     1081 catgttatct cgataatgct cccaatggcc agattgttcc cagagagccc ggtctaaaat
     1141 cataggagtt tttacttcct gataacctcg tctcaggtgt tccctacgcc acaaatctaa
     1201 aagagtatta ataactacca tccctttggg atgaaagaag ggaaagcccg gtccctcttc
     1261 gtgtaagcta aacaaatcca gctctttacc taaacggcga tggtccctct ttttggcttc
     1321 ctctctttga gtcagaaatg cttgtaattt ttcttccgaa tcaaaggaga ttccatatat
     1381 ccgctgtagc atagggttat cttcttttcc tcgccagtaa gctcctgaaa cactcaaaag
     1441 cttaaaggct ttgatgtaac cagtagaagg aacatgtggc ccccgacaga ggtctaaaaa
     1501 ttctccctct tcataaatac tcaccgtatc ttcttctatc tcttttaaaa tttctaattt
     1561 gtaagtttct cctctttcag caaatatctt ctgtgcttct tctcgggaga tttctcttcg
     1621 cttgaaaggc aggtctgctt gaatgatttt tctcatttct tcttctattt tttctaaatc
     1681 ttcatcagtc aaggaacgag ggaggtcaaa atcgtagtaa aaaccttctt caatagctgg
     1741 accaatggcc aatttagcct cagggaacaa ccttttcact gcctgagcca taatatgaga
     1801 agtgctgtgc cagtaaactt ctctacctgc ttcatcttca aaggagagat atttttttat
     1861 tttctcctct tcccctatat cgaccagacg cccatcttcc gcaaccgcag ctacagcttc
     1921 tctagaattt acgttcataa tctttcctcc tcagataata cataacaaca ttctttttaa
     1981 tagaaataat ataatatgag agtagacaac taattaccta aagagtgcca ctttccccct
     2041 gcttactctc tttgcctcac cctgtgttct ctagttaatg gggtatgatg gctacactct
     2101 aatatattac cacattactg agaaatcggg gatataataa agagattgtt ttaataccct
     2161 ggtgtcgtga ctaatcccga gtttttaatg tgatagatgc atccaaaaat gatggataaa
     2221 ggagcaacat actaaatcaa ttttaaatct gaaagcccaa tttaactatt tacgacctga
     2281 aaagtagttc ctatgaatgt gttttcttac cag
//
LOCUS       DAOSVE010000157         2308 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_18826_length_2308_cov_65.353751, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000157 DAOSVE010000000
VERSION     DAOSVE010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18826_length_2308_cov_65.353751"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..1144
                     /locus_tag="PK016_08595"
     CDS             77..1144
                     /locus_tag="PK016_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HXL03151.1"
                     /translation="MAVAVIMAGGRGERLWPLSRSSRPKQFISWKRHSFFRATVDRVK
                     PIVGVENIYVVTQKKYRELILKQAPLIPPSNIIVEPQGKNTAPCIGLAAVIISALKNP
                     EEIMIILPADHFIGDEEAFQKLIRFACRVANDNFLVTLGITPLYPHTGYGYIHYGDSY
                     YKEEIEAFKVLKFTEKPDLETARKYLKEGRYLWNSGIFIWKARVILEEMRKYMPDLYQ
                     GLMKIKESIGTPQEKEVMSEVFKNLENISIDYGIMEKTDRALVIPADIKWSDVGSWAA
                     LEEILKKDQQGNIVFNKHLGIDTQNCIIWTKKPVVTLGIKNLVVVETDDVIFLMPKSH
                     SQRVRLVIEALKEEEEFKKLL"
     gene            complement(1160..2143)
                     /locus_tag="PK016_08600"
     CDS             complement(1160..2143)
                     /locus_tag="PK016_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HXL03152.1"
                     /translation="MSKEELCNFLYREKESSLFRRYEKNPILQPEDWPYEVNSTFNPG
                     ACIFQDKVLLLVRVEDCRGFSHLTIATSDNGKDDWVIEKEPCLFPEAYVHEEQWGIED
                     PRIVFLEEEKQYAVTYVSFSKGGPVVSLALTDDFKHFEKKGELLPPEDKDASLFPRKF
                     KVGSSSPRYALIHRPIIRGEAHIWISFSPDLRHWGDHQILIPTRGGWWDQRRVGLGAQ
                     PIETPEGWLIFYHGVRDTTSGSLYRMGAALLDLEDPTKVIYRTSNWLLSPKESFEWLG
                     DVPGVVFPSGAIVNKEKNEILLYYGAADKYVGLAIGNYQEVLEYLKDSPVQ"
BASE COUNT          763 a          446 c          450 g          649 t
ORIGIN      
        1 gagttttaca gttaactata ataaaataaa acattctgga tcgtatttga taatacccat
       61 aaaggaggtg gcaaatatgg cagtagcagt gattatggca ggtggtagag gcgaaagatt
      121 gtggccccta agccgtagct cccgtcctaa acaatttatt tcctggaaaa gacatagttt
      181 tttcagagct acagtagacc gggtaaagcc tatagttgga gtagaaaata tttatgtagt
      241 aacccagaaa aaataccggg aattaatctt aaagcaggct cctcttattc ctccttctaa
      301 cattatagta gaacctcagg gtaaaaacac cgctccttgc attggactcg cagcggttat
      361 cattagtgct ttaaaaaatc cggaggaaat tatgataatt ctccctgctg accactttat
      421 aggagatgaa gaggctttcc agaaattaat tcgttttgct tgtcgtgtgg caaatgataa
      481 tttcttggtc accttaggta tcactcccct ttatcctcat actggttacg gatatattca
      541 ctatggagat tcttattaca aagaagaaat agaagcattt aaagttttaa aatttactga
      601 gaaaccagac cttgaaactg ccagaaaata cctgaaagaa ggaagatatt tgtggaacag
      661 tgggatattt atatggaaag ccagagttat tttagaagag atgagaaaat atatgcctga
      721 tttatatcaa gggcttatga agataaaaga aagcataggc actcctcaag aaaaagaagt
      781 gatgagtgaa gtcttcaaaa acttagagaa tatctccata gattatggaa taatggaaaa
      841 aactgaccgg gctttggtaa tccccgctga tataaagtgg agcgatgtag gctcttgggc
      901 agctttagaa gaaatcttaa aaaaagacca gcaaggaaac atcgttttta ataaacactt
      961 aggaattgat actcaaaatt gtattatctg gaccaaaaag cccgtggtta ctctgggaat
     1021 aaaaaattta gtagtagtag aaaccgatga cgtaattttc cttatgccca aaagtcatag
     1081 ccaaagagtg agattggtaa ttgaagcttt aaaagaagaa gaggaattta aaaaactcct
     1141 ctaatttctt cctcactttt tattgtacag gactatcttt aagatactct aagacttcct
     1201 gataattacc aatggccagt cctacatatt tgtcagcagc tccataataa agtaatattt
     1261 catttttttc cttgtttact atagctccag aggggaaaac gacgccaggg acatctccta
     1321 accactcaaa agactcctta ggactaagta accagttgct ggttcggtaa attactttgg
     1381 tagggtcttc taaatctaaa agggctgctc ccatccgata gaggctacct gaagtagtat
     1441 ctcttactcc gtggtaaaaa ataagccaac cctcaggagt ctcaataggt tgagcaccga
     1501 gccctactcg gcgttggtcc caccatcctc ctcgagtagg aataagaatt tgatgatccc
     1561 cccaatgtcg gagatccgga gagaaagaaa tccagatatg cgcctctcct ctaatgatag
     1621 gacggtgaat taaagcataa cggggcgaag atgaaccaac tttaaatttt cggggaaaaa
     1681 gggaagcatc tttatcttcg gggggaagaa gctctccttt tttttcaaaa tgtttaaaat
     1741 catcagtaag agctaaagaa accaccgggc ctcctttact aaaggagaca taagtaacag
     1801 catattgttt ttcctcttct aaaaaaacaa tacgggggtc ttctatcccc cactgctcct
     1861 cgtggacata agcctcaggg aaaagacatg gttctttttc tataacccaa tcgtctttgc
     1921 cgttatcact tgtagctatg gtaagatgcg aaaatccccg gcaatcttct acccggacaa
     1981 gaagaagaac tttatcctgg aaaatacaag ctccagggtt aaaagtagaa ttaacctcat
     2041 aaggccaatc ttcaggttgt aaaataggat ttttctcata gcgacggaaa agggaggatt
     2101 ctttctctcg ataaagaaaa ttgcataatt cttccttgga catctcgttc tcctttcggt
     2161 ctaattttct tatattatat tataacctga ctttgacagc tataatttat agttaaccgc
     2221 aaaatccatt ttaatgacta tgaggaggca agcgccctca taaccccgaa ctataaaggg
     2281 acgttgcccc cttataatcc cccaaaaa
//
LOCUS       DAOSVE010000158         2069 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_21905_length_2069_cov_68.820755, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000158 DAOSVE010000000
VERSION     DAOSVE010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21905_length_2069_cov_68.820755"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="PK016_08605"
     CDS             complement(<1..658)
                     /locus_tag="PK016_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007930580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HXL03153.1"
                     /translation="MAKILKKEVLTPQIKLMVIDAPWVACKAKPGQFVVLRINEQGER
                     IPLTIADFGPQEGTVTIIFQEVGKTTMMLGELEEGDEVLDFIGPLGKEIEEKYFGHVV
                     CVGGGVGIAPTYPKARAFKEKGNRVTSIIGARSSNLLFWEDKMREVSDELFVTTDDGT
                     YGEKGFVTQVLERVLNKDKVDLVVAVGPVMMMKMVSLLTEKYSIPTLVSLNPIMVDGT
                     G"
     gene            816..1340
                     /locus_tag="PK016_08610"
     CDS             816..1340
                     /locus_tag="PK016_08610"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HXL03154.1"
                     /translation="MKKLRSILLVTIFSMIFAAGIASAQTISSQPVYYVNFMDTVSKV
                     EELSAFVEVIKAAGLEETLNKGEYTIFAPTNDAFAALPEGKLERLLQPENKETLKDIL
                     TYHVIPRRLSVDDFRAGEKYKTLNGHLIHVFFEKPPIVINQKINVVGSYAASNAIIYT
                     VDKVIFPPEGPKPK"
     gene            complement(1402..1476)
                     /locus_tag="PK016_08615"
     tRNA            complement(1402..1476)
                     /locus_tag="PK016_08615"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1442..1444),aa:Thr,seq:cgt)
     gene            complement(1574..1663)
                     /locus_tag="PK016_08620"
                     /pseudo
     CDS             complement(1574..1663)
                     /locus_tag="PK016_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489362.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          588 a          469 c          351 g          661 t
ORIGIN      
        1 ttcccgttcc atccaccatt atgggattaa ggctcaccag agtgggaatg gaatattttt
       61 cagttaagag gcttaccatc ttcatcatca ttaccggccc cactgctact actaaatcca
      121 ccttatcttt gtttagaact cgttccagaa cctgagttac aaaccctttc tcaccatatg
      181 tcccatcatc agtagtgaca aaaagctcat cactcacttc tcgcatttta tcttcccaaa
      241 aaagaaggtt agaactgcga gctccaatga tagaagtaac ccgatttcct ttttccttaa
      301 aagctcgagc cttgggataa gtaggggcaa tacctactcc tcctcctaca caaactacgt
      361 gaccgaaata tttttcttcg atttcttttc ccaaaggtcc tatgaaatct aaaacctcat
      421 ccccttcttc cagttcccct aacatcatgg tggttttacc tacctcttga aaaatgatgg
      481 taactgttcc ttcctgcggg ccaaaatcag caatggttaa gggaatacgc tctccctgct
      541 cattaatgcg taaaactaca aattggccag gtttggcttt gcaagctacc cagggggcat
      601 caattaccat aagtttaatc tgaggggtga gaacttcttt ttttagaatc tttgccactt
      661 tctttcctcc ttctaaattt agaaaaccaa aaaaattata ctatataatc cctttttatt
      721 tagggtatat tttaaattat accgttttat tttccaaatc ctatgcccta tgttataata
      781 cgctttagca aaccgaaaag aaaggagaag gtcaaatgaa gaaattgcgt tctattttac
      841 ttgtgactat ctttagcatg attttcgctg caggtatcgc atcagctcaa actatctcat
      901 ctcaacccgt ctactatgtc aactttatgg ataccgtctc taaagtagaa gaactgagtg
      961 cttttgtcga ggtaattaaa gctgccgggc tggaagagac ccttaacaaa ggtgaataca
     1021 ccatttttgc tcccactaac gatgcttttg ctgctcttcc agaaggaaaa ctggaaagat
     1081 tgctccagcc cgagaacaaa gaaaccctaa aagacatact cacctaccac gtaatccctc
     1141 gtcgtctctc agtagatgat tttcgagcag gagaaaaata taaaaccctg aacggtcacc
     1201 taattcatgt cttttttgag aagccaccca tagtgattaa tcaaaaaatc aatgtagtag
     1261 gctcttatgc tgcctctaat gccattattt atacggtgga taaagtaatt ttcccaccag
     1321 aggggcctaa accaaaataa aggcgagtac taaaaagcgc cctcttcagg aaataaactt
     1381 gggggcgctt acaattttta gtggagccgg cgagcggatt cgaaccgcta acctgcgcat
     1441 tacgaatgcg ctgctctacc gttgagccac accggctaaa caccgcaaaa attctataac
     1501 tttctctctt ccctgtcaaa cattcattaa gtgaactctt aacattatat aaaaacgtca
     1561 ccttatgtta aacgtcaatg attgcggctg ttattttctc atcataaaat attttattcc
     1621 acttgctgaa ttccaagttt gtagtaattt ttatactgta ctttttaact tccactgtgc
     1681 tgcatttcta ttctaccaaa cacactttgt ctttctctat ggcctttatt tcttttgttt
     1741 tttaagtttt attaatcttg cgtcgcagac tgcctttttc tccagcatac tggtggatat
     1801 ttttccacca gtagtttttg tttatttgct ttttaagtct ttgttctttt cttttgcttt
     1861 ttagggatta taagggggct tgcctcctca taatcgatag aaccattcag gaataacggc
     1921 aagaagacac cctcacccta accctctccc ttcaagggcg aggtaaggaa tatgttaagg
     1981 aatatgtggt gttcttccat tgaaggcgag ggaagatttt gcggttaact ataaagcgga
     2041 atgaggttaa attcttgata tatagtagc
//
LOCUS       DAOSVE010000159         2010 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_22815_length_2010_cov_64.738107, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000159 DAOSVE010000000
VERSION     DAOSVE010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22815_length_2010_cov_64.738107"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(397..825)
                     /locus_tag="PK016_08625"
     CDS             complement(397..825)
                     /locus_tag="PK016_08625"
                     /inference="COORDINATES: protein motif:HMM:NF021634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HXL03155.1"
                     /translation="MRYKVFLDTNILLSGIFFEGNEAKLLNIMEIDLITSEDVVRELK
                     IVAKKKLKFLGRRPLEIALLEIDRALYDVEILPQDAYKDKLSEAKRLIAHEKDVPILA
                     AALYADVDYLLTGDSHFFTDEIKAIIRVRTTREFLDEIGK"
     gene            complement(822..1037)
                     /locus_tag="PK016_08630"
     CDS             complement(822..1037)
                     /locus_tag="PK016_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL03156.1"
                     /translation="MPKVSTDIPDDLYEKIEEEVRLGIFNDISEAINAALRKAYAEKS
                     RSYLRWLAKKEGISEDSMVEELKKIRE"
     gene            1821..1994
                     /locus_tag="PK016_08635"
     CDS             1821..1994
                     /locus_tag="PK016_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL03157.1"
                     /translation="MKGKKQLPEELPSIFSDKRAKTEKEELEAELYRQIGQLKVELEW
                     LKKNLLSLSGGKA"
BASE COUNT          653 a          401 c          374 g          582 t
ORIGIN      
        1 tattatcaaa tacgatccag aatgttttat tttattatag ttaactgtaa aactcattac
       61 aaatacaata aaggggagac cccctttata atcccccgaa aaagaaaaaa catcctgcgg
      121 cacaagatta aaaggcaaaa tcacctttgt ccgtcacccc tgaatgtttc tgcgaggggt
      181 ccacagtatt taaaaacctt tggattccca ataaaagatt ttgggaatga cgaaagatgt
      241 ggattttcgg ccaaatattt caggcatgac gggtgagagg gtggattccc gattactaat
      301 ttcaggcatg acggaggggt gtatgcgtga ctaaatttca gaaatgacaa aaagaaagta
      361 atagaataat atcaaccaaa actatcctgt cttcttttat ttcccaattt cgtctaaaaa
      421 ctctcttgtg gttctgactc taattattgc cttaatctcg tccgtaaaaa agtgggagtc
      481 gcccgttaac aggtaatcta catctgcata aagcgctgca gccagtattg gaacatcctt
      541 ttcgtgggct attagtctct ttgcttctga aagtttgtct ttatatgcat cctggggtaa
      601 aatctctacg tcatacagtg ctctgtctat ctctaacagt gcaatttcaa gggggcgtct
      661 tcccagaaat ttgagcttct ttttagctac tatttttagc tcccgaacta catcctcact
      721 tgttattaag tcaatttcca taatattcag taacttagcc tcgttacctt caaagaaaat
      781 acctgataaa agaatgttgg tatccagaaa tactttatat ctcattctct tattttttta
      841 agttcttcaa ccattgaatc ctcagatatg ccctcttttt ttgctagcca tcttagatat
      901 gatctacttt tctcagcata cgcttttctg agagcagcat ttatcgcctc cgatatatca
      961 ttaaatatac caagccgtac ctcttcttcg attttctcat acaaatcatc aggtatatct
     1021 gtagatacct taggcatttt tatcacctcc cttaaaggag acctcccttt tcccatactc
     1081 taaattaagc atatagctta actgaaattt tgagaaccgt tcgaaaattt ctatcttaat
     1141 tataataatc aaaggggttc tgtcaaactg cagtaatcgg gagaaccacc aacacacgat
     1201 aaacaactcc tgactttgac aagcattttc gcagcgaggc tatcattcga gataagtagc
     1261 ctcctcgtca cccctgaatg actctatcgg ctataggagg gaaaagaccc cctattaccc
     1321 ccttggaaaa acaaaaactt ggattcccgc ctaaatttcg ggaatgacgg cggggggggt
     1381 ggattcccaa ttaaagcgct caggcatgag ggtggggtgg attaccgata aaaatcttgg
     1441 gaatagcaga gttcttcctc tctttgagaa gaagagtaaa attctcccat aaaatacttt
     1501 tttctcaacc tcatttcctt attcctcccg caagagatgg ggagaggagt ctccatattt
     1561 tatccctctc cagagtcctc aagttaaaag gttaccttta caggaagagt tttttatagg
     1621 agtataatgg acccccaaaa ctggacagag taataaggtg tatttaacct gtaaaatgga
     1681 aaggagaaag ataagaaaag gggtaacatc cgaaagaagt atgatgcagc actaaaagct
     1741 aaaatagcac gagaagcacc gaatgagata agattataag agaaatatcc agtgcctatg
     1801 gggtacattc taatcaaata atgaaaggga agaaacagtt acctgaagaa ttaccctcta
     1861 tattcagtga taaaagagca aagactgaga aagaagaatt agaagcagag ctatatagac
     1921 agataggaca attaaaagta gaacttgaat ggctcaaaaa aaatctgtta tccttgagtg
     1981 gaggaaaagc gtaaaatgat agagccgagg
//
LOCUS       DAOSVE010000160         1991 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_23128_length_1991_cov_75.976756, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000160 DAOSVE010000000
VERSION     DAOSVE010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23128_length_1991_cov_75.976756"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PK016_08640"
     CDS             complement(<1..355)
                     /locus_tag="PK016_08640"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HXL03158.1"
                     /translation="MKILLISDLEGASGVVSNEQQAKPGAPLYQEARNYLISDVGGAV
                     EGAIEGGADEVIIFDMHYSGLNLSLEEIHPRAKLVMGKPRKIYPFFSELYQEIKGVFQ
                     DGGRLYLTKKNQQAIG"
     gene            complement(377..1021)
                     /gene="dhaL"
                     /locus_tag="PK016_08645"
     CDS             complement(377..1021)
                     /gene="dhaL"
                     /locus_tag="PK016_08645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HXL03159.1"
                     /translation="MSSIPFEKVKKAFSRIGENLEKHRQYLNDLDSPIGDSDHGESVC
                     NAFHKIQEVVDKFPEEKRDIGELLQTVGKSIVLSGGAAMGPLYGTAFMDAGKAVLGKE
                     ELAPADLVQMWEAFAGGIKRRGQVELGDKTMYDTIYPSFLALRKAYEEGKSLKEMLQE
                     AIEGAREGMESTKDLISKRGRSSRLGERSIGHIDPGAASSFFIIEAFFGVLAED"
     gene            complement(1033..1989)
                     /locus_tag="PK016_08650"
     CDS             complement(1033..1989)
                     /locus_tag="PK016_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HXL03160.1"
                     /translation="MLEGFVIANQNKVKRLETERVLVRKDAPVAGKVGIVTGGGSGHK
                     PAFIGYIGRGLVDAVAVGDIFAAPPVQRVYEAIKATDAGKGVLLCLGNYSGDVMNFGM
                     AQELAQSEGIEVESVIVNDDVASSPKERMDNRRGVAGEIILWKVAGALAEEGADLKKI
                     KEVGERAVFNTRSMGVAHTPCILPTTGKPSFQIGEEEMEIGVGHHGEPGIKRTKMMSA
                     DEVTELLTHQVLEDLPFQRGDEVTVLINGLGSTSLLEMYICFRKVAQILSDYGIKIHR
                     TYVGEFFTSLEMGGFSVTLTKMDEELKRLIDAPCDGVHLIQH"
BASE COUNT          534 a          522 c          322 g          613 t
ORIGIN      
        1 ctccaattgc ctgttgattt ttctttgtca aatacaatct tccaccatct tgaaataccc
       61 cttttatttc ctggtagagc tcagaaaaaa aggggtagat tttgcgtggc tttcccatca
      121 ccaacttagc ccggggatga atttcttcta aactcaagtt tagaccagaa tagtgcatat
      181 caaaaattat aacttcatcc gctccgccct ctattgctcc ttctaccgct ccccctacat
      241 cagaaataag ataatttctt gcctcctgat agaggggagc accaggttta gcttgctgct
      301 cgttactcac tactcctgaa gctccttcta aatcagaaat taaaagaatt ttcacgagaa
      361 taacctcctt agtaaattaa tcttctgcca aaaccccaaa aaaggcttcg atgatgaaga
      421 aactagaagc tgctcccggg tcaatatggc ctatgcttct ctctcctaaa cgactggagc
      481 gaccacgctt agagattaaa tctttagtag attccatacc ttcccgcgct ccttcaattg
      541 cctcttggag catctctttc agggatttac cttcttcata ggctttccgc aaagctaaaa
      601 aagagggata aatggtatcg tacatagttt tatctcctaa ctctacctgt ccccttcttt
      661 ttatccctcc agcaaaagcc tcccacatct ggactaaatc agcaggggca agctcttctt
      721 ttcccagtac tgcctttcca gcatccataa aagcagtacc atagagaggt cccatagctg
      781 cacccccgga gagaacgata gatttaccta ccgtttgtaa aagctcaccg atatctcttt
      841 tctcttcggg gaatttatct accacctctt gaatcttatg gaaagcattg caaacactct
      901 ctccatggtc agaatcacca atgggggaat ctaagtcatt caaatattgg cgatgttttt
      961 ccagattctc cccaatgcgg ctgaaagcct tttttacttt ctcaaaaggt atactagaca
     1021 tagctttctc ctttagtgtt ggatgaggtg taccccatca cagggagcat ctatcaatct
     1081 ttttagttct tcatccatct tagttaaggt tactgaaaaa cctcccatct ctaaagaagt
     1141 aaagaactct cctacatagg tacggtgaat ttttatccca tagtcggaga ggatttgggc
     1201 tactttacgg aagcagatat acatctctaa aagagaggta gaacctaaac cattgattaa
     1261 aacggtaact tcatctcctc tttggaaggg aaggtcttct aaaacctggt gggtgagaag
     1321 ctcggttact tcatcggcac tcatcatttt ggttctttta atccctggct cgccatgatg
     1381 tcccactccg atttccattt cctcttctcc aatctgaaaa gaaggttttc cagtagtggg
     1441 taagatacaa ggagtatgag ctacccccat actacgagtg ttaaacactg ctctttctcc
     1501 tacttcttta atctttttca aatctgctcc ttcttcagcc aaagcaccgg ctactttcca
     1561 gagtattatc tctccggcta ctcctcgccg attatccatt ctctctttgg gagaagacgc
     1621 tacatcatca ttaactataa cactttctac ttctattcct tctgattggg ctaactcttg
     1681 agccatacca aagttcatca catctcccga ataatttccc aaacatagaa gcactccttt
     1741 tccggcatcg gtagctttaa tagcttcata cactctctgc actggaggag cagcaaaaat
     1801 atctcctaca gctaccgcgt ctactaaacc ccttccaata tagccgataa aagccggctt
     1861 atgtcctgag ccacctccag taactattcc tactttcccc gcaactggag cgtcttttct
     1921 taccaaaact ctttcggttt ctaatctctt tactttattc tggttagcta ttacaaaacc
     1981 ttctaacatc t
//
LOCUS       DAOSVE010000161         1897 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_24740_length_1897_cov_83.247014, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000161 DAOSVE010000000
VERSION     DAOSVE010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24740_length_1897_cov_83.247014"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            812..1228
                     /locus_tag="PK016_08655"
     CDS             812..1228
                     /locus_tag="PK016_08655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL03161.1"
                     /translation="MDYQELLYSFRVKIKLFYTGSCGLVKATAYYVSDRIGVMLRGNL
                     VEVGKAKEILDKPLHPYTQFLKQSILEPDPDKKKLDILQKQEISATEISEYQREGCKF
                     SGRCPQVMDACREKEPGYYEPAENGIMVKCFLYESL"
     gene            1294..>1897
                     /locus_tag="PK016_08660"
     CDS             1294..>1897
                     /locus_tag="PK016_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014480512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HXL03162.1"
                     /translation="MYVDRNFTVGRASCRLFGSFIEHLGRAVYTGIYEPDHPKADQRG
                     FRKDVIDLIKELNVSIIRYPGGNFVSGYNWEDGVGPVDKRPKKLELAWRSLEPNEFGT
                     NEFVEWCGLIGAEPMMSINLGTRGIDEARNFVEYCNHLAGSYYSDLRRSHGYEKPHSI
                     KVWCLGNEMDGPWQIGHKTAQEYARLAEETAKVMKIVDPGI"
BASE COUNT          633 a          327 c          426 g          511 t
ORIGIN      
        1 tttttccccc tcccttaccc accctgccaa atcaattatt tggcagggaa cccgggcact
       61 ccctccctcc aggggcggga gaaaaaaatg cgggagactc aatttttccc ctctccctcg
      121 aagggagagc tttacatctt ttttccctcg cccttgaagg gagagggtta gggtgagggt
      181 gtcttcttgt ctgttattcc tgaatggttc tatcaggaat ccagaactat aagggggtaa
      241 acccccttat aacctaatta tgtcccacaa ttgctttctg aacactattt atgattaaac
      301 cgcaaaaaca gcctacggcg tagacaaaag gcaaaaaaca caaaagacat aaaagacaaa
      361 ggccatagag aaagacaaag tctttctcta tgcgtagcga ccttgagttt ttgactttcg
      421 atataattta cttttcaaac gttttaacat tttaaccagt tttgcgagat actataatac
      481 cactttatta ctctgtccag ttttcgaggt ccgttttaaa caatgataac tacaaaaaaa
      541 tcgatttgga aagttgacaa gttttggaga gaatgatact atcgttgcag taaaaattta
      601 tcttaagaga tactaacatg agaaaaaaat ttacagttag gaatataact caggagttaa
      661 agtatcaatg accggtggga acagagttcc taactatagg gagatagcta acccgaattc
      721 gtataaaaag atcatgagaa ggattcataa cataaattgt ttctattatt tctctctcta
      781 tttaaagaat aaaacagcaa agaaaagtat cttggattac caagaactat tatatagttt
      841 cagagtgaag ataaagcttt tctacaccgg tagctgtggc ttagttaaag ccactgccta
      901 ctatgtaagc gataggatag gggttatgtt aagagggaat ctagtagaag taggaaaagc
      961 aaaagaaatt ctggataaac ccttacaccc ttatactcag tttttgaagc aatctatact
     1021 tgaacccgac ccggataaga aaaagttgga cattttgcaa aaacaggaaa tttcagctac
     1081 agaaataagt gaatatcaaa gggagggttg taagttttcg ggaagatgcc cccaggtgat
     1141 ggatgcgtgc cgagaaaagg aaccgggata ttatgagcca gcggaaaacg ggataatggt
     1201 caagtgtttt ttgtatgaat ctttgtagga gaacgaggaa aattaaaaac tttaaagaga
     1261 ggtgctaatg tggtgagtga taaaaaatca aaaatgtacg tggaccgaaa ctttacggtg
     1321 gggagagctt cgtgtaggct ttttggttca tttattgaac accttggtag agcagtatat
     1381 accggtatat atgaacctga ccatccaaaa gcagaccaga ggggtttcag aaaagatgtt
     1441 atagatttaa ttaaagaatt gaatgtttct atcattcgct atccgggggg aaacttcgtt
     1501 tcgggatata actgggaaga tggggttgga ccagtagata aaagacctaa gaagctagag
     1561 ctggcatggc gctctttaga gcccaatgag tttggcacca atgaatttgt agaatggtgc
     1621 gggctaatag gcgcagagcc tatgatgtct ataaatttag gcaccagagg gatagatgaa
     1681 gcccgtaatt ttgtggaata ctgtaaccat ctagcaggga gttactatag tgacttgcga
     1741 agaagtcacg gatatgaaaa accccacagt ataaaagtct ggtgtttagg aaatgaaatg
     1801 gatggcccct ggcaaattgg ccataaaacc gctcaggaat acgctcgttt agctgaagaa
     1861 acagctaaag ttatgaaaat agtagacccg ggtattg
//
LOCUS       DAOSVE010000162         1783 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_27030_length_1783_cov_75.858796, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000162 DAOSVE010000000
VERSION     DAOSVE010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27030_length_1783_cov_75.858796"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..485
                     /gene="smpB"
                     /locus_tag="PK016_08665"
     CDS             36..485
                     /gene="smpB"
                     /locus_tag="PK016_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HXL03163.1"
                     /translation="MKVIAENRKARHIYTIVEKAEAGIELQGTEVKSLRNHAVSLDQS
                     FARVEGGEMWLYDLHISPYDYGNLFNHDPRRPRRLLLHKREILRWGSQVEAKGMTIVP
                     LRIYFNDRGKVKVEIALVKPKKVYDRRQEIQEREEERNLRRVLKTKV"
     gene            486..835
                     /gene="ssrA"
                     /locus_tag="PK016_08670"
     tmRNA           486..835
                     /gene="ssrA"
                     /locus_tag="PK016_08670"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(904..1776)
                     /gene="brxL"
                     /locus_tag="PK016_08675"
     CDS             complement(904..1776)
                     /gene="brxL"
                     /locus_tag="PK016_08675"
                     /inference="COORDINATES: protein motif:HMM:NF024733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system Lon protease-like protein BrxL"
                     /protein_id="HXL03164.1"
                     /translation="MRSMEYNPDGFYGLEQKLLFLSRLLIFIEPRLNMIELAPKGTGK
                     TYIFSNLSKYGWWVGGGIITRAKMFFDISRNTFGFITKYDFVALDEIQTIKFSDESEL
                     KGAFKDYLEQGKFTVANVMGTSNAGVILLGNISLNQYMRPINNIYFSELPDFFHESAL
                     LDRFHGFIEGWNLPRMREDMKVRGYTLNVEYFTEVLHLLRERAEFASAVDEMLDIPRT
                     ADTRDVNAIKRLATAYLKLLFPHVKQASDIDKKDFEIFCLNPAIEKRGIIRQQIHLID
                     PEYKEELPDIKVIN"
BASE COUNT          565 a          331 c          373 g          514 t
ORIGIN      
        1 gggaattttt ggagggtaac cgggttttta agataatgaa ggttatagct gaaaaccgta
       61 aagcaagaca tatttatact atagtagaaa aagcagaggc agggatagaa ttacagggta
      121 cagaagtcaa atccttgcgt aatcatgctg taagcttgga ccaaagtttt gctcgagtag
      181 agggagggga aatgtggctt tatgatttgc acatttctcc ttatgattat ggtaatttat
      241 tcaaccatga ccctagaagg ccaagaagac ttcttcttca caaaagagaa attctccgct
      301 gggggtcaca ggtggaagca aaaggtatga ctatcgttcc tctgcggata tattttaacg
      361 accgcggaaa agttaaagta gaaatagcgc tggtgaaacc taaaaaagtt tatgaccggc
      421 ggcaggaaat tcaagaaaga gaagaagaaa ggaatttaag gagggttctt aagactaaag
      481 tttaaggggg cgtcagggtt tcgacggggc tccttgaatt agaagaggcg agtcgaggtc
      541 tccaccgggg ctcgtaaaaa aggtggtaaa aacataaacg ccaacgataa tttggcactt
      601 gctgcttaat taaagcggca cacccttgag ctcggtgctc acagagttca agtaggtgtc
      661 agatggtgag caaccttctg ggagttctcg ggtaccagaa gcgtaatacc gagatagcaa
      721 gttgctttcc tgcctctccg gaagtaactt gcgaaaccaa aaacagaggc tacactcgta
      781 ggctcttcta actcacgagt ttcggacgcg ggttcgactc ccgccgcctc caccagattt
      841 tatctagttt atttgatata gcttaactaa gatttagcaa gcagaaagga aagattgact
      901 aatttaattt atcactttta tatcgggtag ttcttcttta tactcggggt ctattaaatg
      961 aatctgttgt cttataatac ctcttttttc aatggcagga tttaaacaga atatttcaaa
     1021 atctttttta tcaatgtcag aagcttgctt aacatgagga aagagtaact ttaaatacgc
     1081 tgtagcaagc ctttttatgg catttacgtc tctagtatcg gcggtacggg gtatatcgag
     1141 catctcatct acagcggaag caaattcagc tctttcccga agcagatgta aaacctcagt
     1201 aaaatattcc acgtttagag tataccctct gaccttcata tcttcgcgca ttcttggaag
     1261 attccatcct tctataaaac catgaaaacg atcaagtagg gctgattcgt gaaaaaaatc
     1321 aggaagttct gaaaagtaga tattattgat gggtctcata tattggttta gagagatatt
     1381 tcctaaaaga ataactccgg cattagaagt tcccattaca tttgctacag taaattttcc
     1441 ctgttctaaa taatccttaa aagctccttt taactcgctt tcatcagaaa atttgatagt
     1501 ttgtatctca tctaaagcaa caaaatcata cttggttata aacccgaaag tatttctaga
     1561 aatatcaaaa aacatctttg ctctggtgat gattcctcct cctacccacc atccatattt
     1621 actaaggtta ctaaagatat atgttttgcc tgttcctttg ggagcaagtt caatcatatt
     1681 taaacgaggc tctataaaga ttaaaaggcg agacaagaaa aggagtttct gctctaagcc
     1741 atagaaacca tcagggttat actccattga ccttattaaa agg
//
LOCUS       DAOSVE010000163         1707 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_28718_length_1707_cov_60.694915, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000163 DAOSVE010000000
VERSION     DAOSVE010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28718_length_1707_cov_60.694915"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1348
                     /locus_tag="PK016_08680"
     CDS             <1..1348
                     /locus_tag="PK016_08680"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXL03165.1"
                     /translation="LAGFTEYYNLFVNVGYNLGSRKYKGEKNMTSRERVITSLSHQEP
                     DSLPLDLGSTLITGIHVSSLHKLKVALGLIKPEEPVKVIDPFQMLGEVDDELRKVLGI
                     DTVPLLPLYSFFGFKNENWKPWTFFDGTPLLVPEKFNTTPDSEGNIYQYPGGNTTFPP
                     SAKMPQGGFYHDVIIRQKSLEEEELKVEDQIEEYGLLSEEELRYYESESKRLCEETEY
                     SIVSGGVPGINLGDIAYVPGPALPDPRGIRDVEEWYISLLTRQDFIREVFSRTTDIGL
                     QNLELFYQAVGERIQVIVVSGTDFGSQNGPFISREVYRKLFKPFHQKINHWIHQNTTW
                     KTFIHTCGSVYDLLPDIKEAGFDILNPVQISAAKMIPQNLKKNFEHQFTFWGGGVNTQ
                     KTLPFGTPEEVREEVRQLIETFKPGGGFVFATVHNIQANIPVENLLALFEAVNEYR"
     gene            1472..>1707
                     /locus_tag="PK016_08685"
     CDS             1472..>1707
                     /locus_tag="PK016_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase"
                     /protein_id="HXL03166.1"
                     /translation="MEEATLGVVWPAFYEPSPARWKREKMVKDDRGADHFALARVREK
                     EPELKRDYVGVAPLYYVFNERGELCFSSEVKGLLS"
BASE COUNT          531 a          282 c          396 g          498 t
ORIGIN      
        1 tttagcaggt tttacggaat actataattt atttgtaaat gtcggttata atttaggaag
       61 cagaaaatac aaaggagaaa agaatatgac ttctcgagaa cgagtgataa cttccctttc
      121 tcatcaggaa ccagatagtc tccctttaga tctaggttct accttgatta ccgggattca
      181 tgtttccagc ttgcacaaat taaaagtggc tttaggcttg attaagccag aagagccagt
      241 aaaggtgatt gacccttttc agatgttagg ggaagtagat gatgagttgc gaaaagtttt
      301 gggaatagat actgttcctc tccttcctct ttatagcttt tttgggttta aaaatgaaaa
      361 ctggaaaccc tggacttttt ttgatggaac tcctctttta gtaccggaaa agtttaacac
      421 taccccggac tcagaaggta acatttatca gtatcccggg gggaatacca cctttcctcc
      481 atcggctaaa atgccccagg gcggttttta tcacgacgtt attatcagac agaaatcttt
      541 agaagaggaa gaattgaaag tagaagacca gatagaagag tatggcttgc tttccgaaga
      601 agagctccgc tactacgaaa gtgaatctaa aagactctgt gaagaaactg agtatagtat
      661 agtatcggga ggagttccgg gaatcaatct gggagatatt gcctatgttc ccgggccagc
      721 tttaccagac cctcgaggga ttagagatgt agaagaatgg tatatatcgc ttttaacccg
      781 tcaggacttt atcagggagg ttttttctcg tactaccgat attggtttgc agaatttgga
      841 attattttat caggcggtgg gagaaagaat tcaggtaata gtggtttcag gaactgattt
      901 tggttcgcaa aatggtcctt ttatttcccg ggaggtatat agaaaattat ttaaaccctt
      961 tcatcaaaaa attaaccact ggattcatca aaacaccacc tggaaaactt ttattcacac
     1021 ttgtggttca gtttacgacc ttctcccgga tataaaagaa gctggttttg atatcttgaa
     1081 cccggtgcag atatctgcag ctaagatgat tcctcaaaac ctaaagaaaa actttgaaca
     1141 tcaatttact ttttggggtg gaggagtgaa tactcagaaa actcttcctt ttggaacacc
     1201 tgaagaggtg agagaagaag tgcggcagtt gatagaaact tttaagcctg gtggagggtt
     1261 cgtatttgct acagttcaca atattcaggc taatataccg gtagaaaact tattagctct
     1321 ttttgaggca gtaaatgaat atcgataaaa taggaaggtg aatgtatttt ggctggtatt
     1381 gcaggaattt ttgcttcagg aaagaaaaaa gaagtagaaa aatgctaaat aagactctca
     1441 tcgaggtccg aaaggtaaag cagttttaga attggaagag gcgactttag gagtggtgtg
     1501 gcccgctttc tatgaaccat cgcctgcccg atggaaaaga gaaaagatgg taaaagatga
     1561 tagaggggcc gatcattttg cgctggcacg cgtaagagaa aaagagccag agttaaagcg
     1621 agattatgta ggagtagctc ctctttatta tgtatttaat gaaagaggag agctttgttt
     1681 ttcttcggaa gtaaaaggac ttttgtc
//
LOCUS       DAOSVE010000164         1619 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_2171
            NODE_30964_length_1619_cov_66.241049, whole genome shotgun
            sequence.
ACCESSION   DAOSVE010000164 DAOSVE010000000
VERSION     DAOSVE010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864238
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,739
            CDSs (total)                      :: 1,700
            Genes (coding)                    :: 1,675
            CDSs (with protein)               :: 1,675
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 9 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30964_length_1619_cov_66.241049"
                     /isolate="AMR_MDS_2171"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PK016_08690"
     CDS             <1..227
                     /locus_tag="PK016_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL03167.1"
                     /translation="SMFFYSGEESFLLRYNGTPRKGVIVNRKIHYKYYKGECPFIIPQ
                     KTKKYKRKKLLRKEKRFPQYLRHQRRKQNR"
     gene            complement(289..>1619)
                     /locus_tag="PK016_08695"
     CDS             complement(289..>1619)
                     /locus_tag="PK016_08695"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HXL03168.1"
                     /translation="AMLFQRLLQPDSKLALLEWLPLTPLKHFLAPGELDLQSLYRSLE
                     ILQANLKPVEEYLYHMATVQFKEESKELYYDLTSSYFEGRKCIIAEYGYSRDKRKDRK
                     QIVIGLVTTASGFPLKCKVYPGNRVDKTTVTEMISELQEEFPLPEIVLVGDRGMLTAN
                     NVATIEELGHKYVMALPRVQSKKYLKDRDVDLKSMRKITTDLYAQFIPGEENQRLLLC
                     LNTQKREEDREYRQFCLESIQEELERLNNSLGKNKTITSRDEAMKRVGMILKENFARK
                     YFRVATVNSSKNPLGFEIQYQLKTAQIEEDEKLDGTFMICSNEQSYEDEKLIQVYKNL
                     SQVERAFKVIKNELDIRPINHRKPVRVEGHVYLCVLAYFITNVIEYLANQKKLNKSAP
                     KILRELSQIALIEIYLPDGQKKYSLTTMQEEHKKILNAFQIKNMEIPDVG"
BASE COUNT          485 a          324 c          278 g          532 t
ORIGIN      
        1 tgtcaatgtt cttctattct ggtgaagaat catttctttt aaggtataat ggtaccccta
       61 gaaaaggggt tatagttaac cgcaaaatcc attacaaata ctataaaggg gagtgcccct
      121 ttataatccc ccaaaaaaca aaaaaataca aaagaaaaaa actactgagg aaagaaaaac
      181 gctttcctca gtacctgcgg caccaaagac gaaagcaaaa tagatgaaac cgaagttcaa
      241 cccgatgttg gcaaataagc ccttgttgag ggattttaag taaaaagtct accctacatc
      301 aggaatctcc atatttttaa tttggaaagc attaagtatc tttttatgtt cttcctgcat
      361 agtagtcaga gaatacttct tttgaccatc aggtaaatag atctcaatca aagcaatttg
      421 acttagttct cttaaaattt taggagcaga tttattaagt ttcttctgat tagcaaggta
      481 ctcaattaca ttggtaataa agtaggccag aacacataag tatacatgcc cttccacccg
      541 gacgggtttg cggtgattaa taggtctgat atctaattca tttttgatca ctttgaaagc
      601 tctctctact tggcttaagt ttttgtaaac ctggatcagt ttttcatcct cataactttg
      661 ctcattagaa caaatcataa aagtaccatc taatttttca tcttcctcaa tttgggcagt
      721 tttgagttgg tattgaatct caaatcccaa agggttttta gaactattca ctgtggctac
      781 tcggaaatac ttccgggcaa aattctcctt gaggatcatc cccactcttt tcatagcttc
      841 atctctactg gtaatagttt tgttctttcc taaactgtta ttcaacctct ccagttcctc
      901 ctggatactc tctaaacaaa actggcgata ctctctatct tcttctctct tttgggtatt
      961 gagacataaa agaagtcttt ggttctcttc tccggggatg aactgagcat agaggtcagt
     1021 ggtaattttc ctcatagatt taagatcaac atctctatct tttaagtatt tcttactctg
     1081 aacacgagga agagccataa catatttgtg tcctagctct tcaatggtgg ccacattatt
     1141 agcagttaac attccccgat ctcctactaa gacaatctct ggaagaggaa attcttcctg
     1201 gagttcagat atcatttctg taactgtggt tttatctact cgatttccag gataaacttt
     1261 acatttgaga gggaaaccag aggcagtggt aaccaatccg atgactatct gtttcctatc
     1321 tttacgctta tctcgggaat agccatattc cgctatgata catttacgac cttcaaaata
     1381 actactggtt aaatcataat atagctcctt gctctcttcc ttaaactgta cagtagccat
     1441 atgatataaa tactcttcta ctggctttaa gttagcctgt aggatttcta agctgcggta
     1501 taaactctgg agatccagtt ctccaggagc taaaaaatgt ttaaggggag ttaggggtaa
     1561 ccactctaaa agagctaatt tactatctgg ttgtagtagt ctttggaaga gcatggctt
//
