LOCUS       DAOSWN010000001        61011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_300_length_61011_cov_5.381439, whole genome shotgun sequence.
ACCESSION   DAOSWN010000001 DAOSWN010000000
VERSION     DAOSWN010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 61011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 61011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61011
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_300_length_61011_cov_5.381439"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..101)
                     /locus_tag="PLF29_00005"
     tRNA            complement(26..101)
                     /locus_tag="PLF29_00005"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(66..68),aa:Ala,seq:cgc)
     gene            complement(175..1881)
                     /locus_tag="PLF29_00010"
     CDS             complement(175..1881)
                     /locus_tag="PLF29_00010"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HXK52362.1"
                     /translation="MNVFTEISIIIFIAMTMAGIMQLLKQPLIMGHILTGLVAGPYFL
                     NLTHSSETIETFSQIGIAVLLFIVGLGLSPKIVREVGKIAVITGVGQVLFTTFFGSLI
                     ALAFGYSLVETLFIAVALTFSSTIIILKLLTDKKDVEKLYGRISIGFLLVQDIIATII
                     LVVVSTASKGDMNAYTLILFTILKGAVLTAILSFISIKILPTLSGFFAKSQEFLFLFA
                     IGWGLGLANIFAYMGFSIEIGALIAGVTLSMSPYNHEIGSKLKPLRDFFIIMFFVLLG
                     MKMSLDSIATILPQALVFSAFVLIGNPVIVMVLMGLLRYNKKTGFMAGLTVAQISEFS
                     LILILLGVQAGHLSQKILSLVTVVGLITIAGSTYLIIYSETIYAKLAKYLVIFERKSS
                     KSEHYKISSPEVVLFGCNRVGYDFLKLFKELGNGFLAVDFDPLIIKQLNAQGFNCIYG
                     DAEDAELIEALNLQNVKMIISTIPDYETNTFLISKAISINPSIAAIVMSHNLAEALQL
                     YDKGAAYVILPHFIGGHFSSMLAGKHWAGVDDLLKQREKNIEYLKNRVELGHTHPTKD
                     HS"
     gene            complement(2233..2904)
                     /locus_tag="PLF29_00015"
     CDS             complement(2233..2904)
                     /locus_tag="PLF29_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52363.1"
                     /translation="MSIKEEITHGSEGRKRIEGEYFLPTASNRGLKDCGVFAAFFCAH
                     NFRGIQYRPLNDMRAEIGKEKDLGTLPDKILKFLATQGLQCEYRTQVDWEEFLHFDSN
                     KLEDVARELDGSDGVLVTASGLKECANSLVYEHSFSLWETDEDPEITFKLALTGGAFV
                     ICLVGANHYVVIVRQEDEAYVYYDPNDPSKLQESKKTDFFQWWEQTTGFVRLREAIIV
                     KNTEE"
     gene            complement(2934..3009)
                     /locus_tag="PLF29_00020"
     tRNA            complement(2934..3009)
                     /locus_tag="PLF29_00020"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2974..2976),aa:Arg,seq:gcg)
     gene            complement(3110..3709)
                     /locus_tag="PLF29_00025"
     CDS             complement(3110..3709)
                     /locus_tag="PLF29_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52364.1"
                     /translation="MNLNLKYLSEVMGFTTRKEENIYQREQELKEKARPKKSDRKTIY
                     EWEALARPHRKPLSQKFLRTLMVIGVFFAALLIAAKEFTLIALILGIYILSYALTKNA
                     PETVLYKITNQGLEIEGKFYFWDELKHFFIFDRDDLRVLGIDAYEAFPPRIYLTLTGS
                     KIDEQELIQLLSVHMDMLEEMPENFLDKAYRELISKLNV"
     gene            complement(3900..4322)
                     /locus_tag="PLF29_00030"
     CDS             complement(3900..4322)
                     /locus_tag="PLF29_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006890203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HXK52365.1"
                     /translation="MDWYITVLKKYADFSGRAGRSEYWMFQLINVGIYFALAFVMGVS
                     SNINTNLGAIFSIVYYVYMFAVLIPSIAVSVRRLHDTNKSGWLLLLSLIPFIGGIIVF
                     VFTVLPSDAGPNAYGEAPAAPVVSNSAPQATAESEPKA"
     gene            complement(4445..4732)
                     /locus_tag="PLF29_00035"
     CDS             complement(4445..4732)
                     /locus_tag="PLF29_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52366.1"
                     /translation="MHEIILKFIIFAYGATSLVDAIAYWPTIKDLWFHKKPSANSRSY
                     QVWTLSAIVTFLYSLFVLPDVLFRVVSLVMLLLNVLILVLRLRLQTFKQQI"
     gene            complement(5234..5407)
                     /locus_tag="PLF29_00040"
     CDS             complement(5234..5407)
                     /locus_tag="PLF29_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52367.1"
                     /translation="MNNKRFSLEQIHSLSIAMALGVFVILSLLDVDSSALKAVGFLFI
                     FELAFKIIYKPKK"
     gene            5555..5959
                     /locus_tag="PLF29_00045"
     CDS             5555..5959
                     /locus_tag="PLF29_00045"
                     /inference="COORDINATES: protein motif:HMM:NF024886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HXK52368.1"
                     /translation="MLNSFMQTELQKTERELKINNYSFKTSKSCLYGLRKYFSFKENN
                     LAEFELDSIKNFLLQCQQKRISPQSRNLFLNAIKFYYRNVAKNIQKIDIRSAKEPKSL
                     PAVLSRSEVKRVLESQKTLSTSFCFHLLTGQG"
     gene            5979..6206
                     /locus_tag="PLF29_00050"
     CDS             5979..6206
                     /locus_tag="PLF29_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52369.1"
                     /translation="MTVQNLDLEELTVHVKQAKGQKDRISVIPKALVTGLDNLTTAKK
                     PGTLSLKANVGENYPPEQHKKFLKMLYEHLV"
     gene            complement(6503..6590)
                     /locus_tag="PLF29_00055"
     tRNA            complement(6503..6590)
                     /locus_tag="PLF29_00055"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6555..6557),aa:Ser,seq:gct)
     gene            6669..7736
                     /locus_tag="PLF29_00060"
     CDS             6669..7736
                     /locus_tag="PLF29_00060"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HXK52370.1"
                     /translation="MTSAPVDNNVNALKTQNMVERAQKTHILQTDVWGDFKTAMGTKA
                     IRVAGVQYTLHRLPKTNIAYAYCPKVNPKNIIWEELEKSLISNRCFAIKFDVPNIIST
                     AANYNNSNTKELEIFTSKKGCIKTYKSTITQNNVLLNIEEEENKLLSNMHPKHRYNIK
                     YAQKKGVQVRITPYNNYDEQAKRDFEIFWNLQKETRARQNFLTHSKNYFEKAFETLGK
                     QNAAFLITAEYRETPLTSWMVFIKDGTLYYPYGGSSEKYRNFQASTLTGWEAITLGKK
                     FNCDLFDMWGACKDLTKESDPEVGFTTFKIRYGGTYVRFIDSYDYVLNKPIYHAFKNI
                     YPKVLGGLKFAAKIKNLGKTS"
     gene            complement(7772..8344)
                     /locus_tag="PLF29_00065"
     CDS             complement(7772..8344)
                     /locus_tag="PLF29_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52371.1"
                     /translation="MLILLFLMLLFFILVVAGSLVFLELFTFVYTTYLCDAPYLPLDK
                     KNIDLALEVLQLSGNDVFVDFGSGDGRVLLSALREHGVKRAIGVEKDRYQALKSKIFL
                     KLRYKGYGTYCVYREDIFRPSTKLLEDLSQITAVYMYLFPSMIDRIYIQLLEQKRPFR
                     IVCCRYSLSGIPPTSVIKGEEFDSYYYEIG"
     gene            complement(8344..11241)
                     /locus_tag="PLF29_00070"
     CDS             complement(8344..11241)
                     /locus_tag="PLF29_00070"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HXK52372.1"
                     /translation="MKKGTFVLNPEQQAAVDHAVGPLLVVAGAGTGKTRVITERIKKL
                     IQHDNLPPEKLLALTFTDKAAAEMLARLDTVMPLGYTEPWVDTFHSFCDRVLKLEGLE
                     IGLNPSYKILSYSDQWVLVRRHLFDFELDYYRPLSNPTKAISALLKFVSRLQDENTSV
                     EDLQSYVESYVGEPEEIKRWKELSSFYKKYTELKLQQSAMDFGDLIVWTIKLFKERNN
                     ILNRYKKQFTHVLVDEFQDTNYAQYELIKLLSPSAELEVEKRSLMVVGDDSQSIYKFR
                     GAAVSNILDFRADYPNTTMLTLLTNYRSSQAILDPAYSLIQKNNPDTLEIKLNISKEL
                     VSSFGADFSGSIKPEIIELDTLESEAEFIVKKILLILGQEPSYSYKDFAILARANNHL
                     DPFIMALRRHGLPYQLVGNRGLYDTDEVRNVIALLRILVDPYDAISLYRILNLQHFNI
                     SPDLISKILAEVKKERVDLWDKLVSTPVEEVQSFLKLFEDYRGRLASQTPSQLTYELV
                     HAVGMIKRLTSEESLENELRLKNLDLFLEKIKKFELSHYADTREIATVVDLVNYIDLM
                     LEAGDSPAQAEIQDYDTVQLMTTHASKGLEYPVVFMINLVSGRFPTRNRSDAIDLPDS
                     LVKERLPSGDAHLQEERRLFYVGMTRAKKYLYLTYAKSYGGKRNTVPSGYLAETKIET
                     TQLSSQDFEGAESQSTLFDKNSVYRKPKPKKNLNEKKLVLSYSKMESYLSCPFRYRYE
                     HVLNVPTPSKYSLSFGNTIHNTLRDYHMKKLVSPSVKIEDVLQMYKANWDPTGYQSKE
                     QREGYYKKGEDVLKKYFEVDILNSAKTFALEKNFYLSLDQDKTLNGRIDRIDRLDSED
                     GVNEVEIIDYKTGQSKSQKEVDENEQVTIYALAAKEVLKLVPKKLTLYFVEHNEKVST
                     TRTLDQLEAIKTKALAVFSKIQDNKFDPTPGHVCKLCPYSDICPHAQKVSY"
     gene            complement(11290..12372)
                     /locus_tag="PLF29_00075"
     CDS             complement(11290..12372)
                     /locus_tag="PLF29_00075"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HXK52373.1"
                     /translation="MSTSTLIIFALALVALGIIYYINTALKKLKEELQAPKEDTTLIE
                     WLKEMKGSVDKNADVIASQLKDQRESLNVQLKNQQDSLQKAQKLMWDRLDNATKVISD
                     VQKNLGGLQEFSKDMKDLSNVLKSPKLRGGLGEQFLYDVLANALPKDLYKTQYKFRTG
                     DVCDAIIKTDKGLIPVDSKFPMENFKLMQTQDSPEEREKAKKTFIRDVKKRIDEVATK
                     YILPAENTTDQAVMYIPSENVYYELLVNTAEIEDYAKAKNVVLASPNTFSYFLKILLV
                     AYQQQELQQHAGVILKALGGLKVEAKKFDSDLGVLDGHLRRATNSMDTVKTNYQKIIN
                     KLENIQQVKPAEDSSKLGLEQKNLLE"
     gene            12482..12775
                     /locus_tag="PLF29_00080"
     CDS             12482..12775
                     /locus_tag="PLF29_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52374.1"
                     /translation="MPKFLYTLTVVILVSWLTLLTYMKTVPPKNIPAALLWGILFFIT
                     TYSTTSVLVYIFNKGKKSDKTDRELYRQGLRASTAIALILTLTITIKIVLKLI"
     gene            12870..14315
                     /gene="metG"
                     /locus_tag="PLF29_00085"
     CDS             12870..14315
                     /gene="metG"
                     /locus_tag="PLF29_00085"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HXK52375.1"
                     /translation="MEKRFYVTTPIYYANGSPHIGHFLTTTIGDVLARYNRLIVGAEN
                     VYFTTGIDEHGTTVSDAAKKAGYEENIQAYVDEAAQEWKAVFDKTHISYDYFVRTTSP
                     YHAKFVKELVLKMQKAGDIYKGNYEGTYCNGCEKHLTLSDLNEEGYCPLHRPDQTIKI
                     TEENYFFRLSKYANRLVEIFEKDSVVIFPESKKNEILSRLKSGVEDLSITRPKEKVSW
                     GIEFPGDSEQTLYVWADALSNYISSLVINNNYDYFWGKYTTHLVGKDINWFHSVIWTA
                     FLLSAGYPLYKNIFAHSFLNIGGKKISKSLGNLITPDELIKYFGVDGARYLVLKNIPY
                     KDDIDVTIETLTQQYNADLANGLGNTVARVTKLAENSQISFDNISEAQSRKEILETIQ
                     EDIEQVKVHEALSKIWLKLSDLEKHINTNEPWAIEDKNQLKTILSHELRELYSIGILL
                     EPFLPETANQIVSSVSSSKIIKDKALFERIK"
     gene            complement(14540..14899)
                     /locus_tag="PLF29_00090"
     CDS             complement(14540..14899)
                     /locus_tag="PLF29_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52376.1"
                     /translation="MKKKDSISSIFDDKPSPGRSFLSGKKIMHMALFVLLFLLGLFIK
                     YILMPNFDSRIFHSAVSQPSGTEDEKKDFAIEPEEDISQGAEIKERVKVIEGSIESLD
                     TLPNNEDLMLDIPEIGM"
     gene            complement(14912..15634)
                     /locus_tag="PLF29_00095"
     CDS             complement(14912..15634)
                     /locus_tag="PLF29_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52377.1"
                     /translation="MHVWLKKALVFSILLFVISLLSQSVRAQDVDQRSDYLEVVKKCN
                     EENDTSAVCNRYRQQALIFSKNRLANTFEAFENRLNRLLGSSEIWDDAELSDLKILKA
                     DFYTFKKENLELVNEAETVGDVEIVVSNLQPAYSVLLNKLNSFIYKESLLKQKVVLDK
                     IVSLIDVLEDTQATYSQEDKDRLADLSYRAQELMVNIDALLENHDNISSSSFNRDLTE
                     NTEDIDSLFDDLRELLNSLGYI"
     gene            complement(15684..16355)
                     /locus_tag="PLF29_00100"
     CDS             complement(15684..16355)
                     /locus_tag="PLF29_00100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HXK52378.1"
                     /translation="MQLNAQKREVFGKKTKSLRENRLIPAVIFGKGMESVPVSINYVD
                     FVRVNNKAGETNVIDLMVDGTEYKVLTKGIAVHPVTSTILHVDFHKVNLKEKISATIP
                     VEIVGEEKNPLVKNGQGLVLVLLNEIDVEALPTDLPNNFTVDVSGLVEIDQGITVKEL
                     NYDRSKVEIKDYEDEDLVVKIGYAVQEEIVEETEEDALSKIEATEETAKDETDEGSST
                     DEKND"
     gene            complement(16301..16654)
                     /locus_tag="PLF29_00105"
     CDS             complement(16301..16654)
                     /locus_tag="PLF29_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52379.1"
                     /translation="MTSSVSLKNQLSKAFCTKCGTSLDSAKFVPISEMPLITIAHAVC
                     SNCRAENMVTITSIGLGVLPLESDLMSSEVRKYINVPAVSLEEVLQVHKKLKKESVCN
                     LMHKKEKSSVKKQKA"
     gene            complement(16746..20066)
                     /locus_tag="PLF29_00110"
     CDS             complement(16746..20066)
                     /locus_tag="PLF29_00110"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXK52380.1"
                     /translation="MPNTNVIKKSALSPTVLIVGGAGFVGSHLAEALLLKDARVIVVD
                     NFSTGKQIYVNKFLENPKFALFEADINVGLPKEIESVDYVYHLAGLEAYLTDASSLSY
                     YTLLTNSVGTKVILDFCKACNAKFMLASLVDVYKGLISPAGLDHYYGTSKEVENKYTL
                     NEAKRYAEALTWEYYKEFQSDVRIARFSEIYGPRMSFEASGSLGKAIKNTLENTDIVI
                     YGDGVEKENYIYISDAVSGLVKGMFSDNTSGEIFTFISEEYSTVLETAYLLKNLASSE
                     IEVTFKPKTTSYAGVDKITLDSAALKKIKWSQKVYLKEGLKKTLESFGYDVSSHSFKP
                     ASLISKKKNPKSSDEVKIDSLVDETSMGIDDVSIKPGEKPNTPVSTNVPAPEDKNAVL
                     ITSQNQISNNPSTIEGISTVDSYGSEKGAPVSSLSILAPEKQFSVKSKPNRRFFSFRK
                     TARDKSLSTKPSQLETLYSSSNLGDSGSSSIVSSADRGGFLSSKKVQFVLSLALVFLA
                     FLIVFVIMPGLQIALHAMRAEGFAQEAISATRSMRLDDAISNSEKAFNELYRADAALS
                     RTYYLFKVVGREDLFTDTRQALNSGKYSIKSIYFLASAGMPLKDLWDTMKPTSENPID
                     TSKIVEATEDLEVAKENLQLAAYELDQIDYPKLKNRTASYKEQLQNALYLSDLASPFL
                     EQFPSIIGVDGTKSYLVLFQNNNELRPTGGFIGSYAVVNFKDGKIASIVIDDVYNPDG
                     QIALREIKIDSPAPIKTYLQEDSLFIRNANWYPEFSQSAEVIKELFFKVDGRNFDGVF
                     AVDLNMAKEFLEITGPIFLTAYNEEITAQNIYERTQFHSEFTFEAGSEQKKTFLTVMG
                     GKLLESVFGLSDTKLPKMVLATKKLLDERHLMVYLPGSSLGTVFNDLGWDGAIRPTGG
                     DYLQVVNANLGGTKANYYIKNEMAYSVTSLTRDGVLRARLELLYDHTGTDSAWPGGPY
                     TNYVRIITPEGTFLTGAKISANDVETNVFDSVVAEKEGAYNSFGVGFKLDPQKKLKVI
                     FEYDLPEMLNISKENGEYSLYWQKQPGTNDDLMSFSFDGPFGTSISSDDPRLILGSLS
                     GSYSGYLNTDFTTSLKIN"
     gene            20438..20902
                     /gene="greA"
                     /locus_tag="PLF29_00115"
     CDS             20438..20902
                     /gene="greA"
                     /locus_tag="PLF29_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HXK52381.1"
                     /translation="MNDQQILISAEGLENLKKELQDLIELKRPEIAKNIQMARDQGDL
                     SENAQYDAFKHEQGLVEGRIKELEDIIKKAKIIEESESNGTVKIGSTVTIHVEGEKEV
                     IKIVGSMEADAMNKMISHESPIGTALLGKKVGDVAEVSAPVGKLKYKILQID"
     gene            complement(21187..22443)
                     /locus_tag="PLF29_00120"
     CDS             complement(21187..22443)
                     /locus_tag="PLF29_00120"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXK52382.1"
                     /translation="MLLPIISLFLAGCTIQDLPVIGGLFGGGGKTTEPVTLVFWGLWE
                     DSGVMNVLIQKYQEQNPNVTINYEDRSIMTTVDYKERIFTRSQQSSGIDLALVHNTWV
                     PDLVTLLAPMPKNLMDVSQYTTSYYPVAAQSAVFDGEIYAVPAYYDGLVLVYNKKHFK
                     EIGQNFPPTAWEEFRRLAIELTVRGPDGELKRGGAAIGTAKNIDHSTDIVGLMMAQAG
                     IAVPEQMDTKPAQDALTFYTNFAKVDKVWSEAMPNATEAFTKEQASMIFVPTWQILDI
                     LKAMPNINDVGVAAVPQALIDNPVSWGSFWMYAVPSNSTNSAVAWDFINFLAQPEQQQ
                     LYFSEASKIRAFGPPYSAVSLKDTVANPYITPVLDSAPFAKSSEIAGRAGNRRQADAL
                     NTSIEEVLSGASAKDALSRAKVEIEK"
     gene            complement(22737..24146)
                     /locus_tag="PLF29_00125"
     CDS             complement(22737..24146)
                     /locus_tag="PLF29_00125"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HXK52383.1"
                     /translation="MQIKNIKLNFMVQLLVVIVFAIPLNLGKHFIIPSSYVNGLLVDY
                     LIPTLYVQDILVFCFSLVFIFEATKSKNRANYKSSFSKINKSWLIPLATIFIFSFGLS
                     SIGSVNITASLVAFLRFSLYTFFCFSAVIVLLESDKSENLPLSTGGNLEDIELGAQTF
                     YSNYPVFLKVAFFSLFLFLVLGTVQLVLQRSVFNNYLVLGEQPYTSATFGISVETFFG
                     NKYVPPYSTFRHPNVFAGYVGLLVFFLFTNLKDLRNLVSRKLLVLCFTMCVIVLVSAS
                     SFNAFLAILIGFLLYYGFSSSANNKNRQAGVTLAIFILSIVISFSFLINTVPDFTSVY
                     SPSVVVRAALASKSKHIIIDNSLFGTGLATSPQLLESYYFERERRFPQPVHNIFLLLG
                     SETGLIGLVLFLSFFLYTLRFFYINKNYGSLCFVLLYFLLGLFDHFIFTSHQILLLFW
                     TFLSLSLYRSEKSLARHQE"
     gene            complement(24173..24943)
                     /locus_tag="PLF29_00130"
     CDS             complement(24173..24943)
                     /locus_tag="PLF29_00130"
                     /inference="COORDINATES: protein motif:HMM:NF019305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52384.1"
                     /translation="MKLRIGYFYKDLLNLYGDNGNVEILVSRCKKRNIDVDVYEVGLE
                     TKLNKFFMSGIDLVFMGGGPDSSQKEMYKDLTTSKASFLRDYIISGGVSLFVCGSYQL
                     LGHYYKSADGSTLQGLGIFDLSTEHPGNSAARCVGNVVCTMSSTITGDPSFPHESGFG
                     GSLVGFENHGGRTNLGTKSSEFAVIRRGFGNNGKDGTEGIHFMNSIGTYLHGPVLSKN
                     PHLADFLIAKSLELPASGLAKLDDGLILNAHNIALDLN"
     gene            complement(24940..26070)
                     /locus_tag="PLF29_00135"
     CDS             complement(24940..26070)
                     /locus_tag="PLF29_00135"
                     /inference="COORDINATES: protein motif:HMM:NF019952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HXK52385.1"
                     /translation="MRFALSKFIVLIIFNLSTRLGIGSGYVWPGHVVLKLFPSILSFK
                     QLQCRKGYILISGTNGKTTTAKLITHILEQSGYKVLNNKTGANLLNGLVTTILLADIG
                     SVDYCVLEIDEFTLPRFLEYIDPKAIALLNLSRDQLDRYGETDIVLDRWQQSIRKLPP
                     SVRIYADSAEPRFKTLSADMHFFDDSSERLSLTSLKGSFNAKNINAATKLVQDLGIPL
                     TGILSSLDSFESAYGRGEQIDFFNKKYHLYLAKNPASFTHNLDLLEHFSPDNTDVLII
                     LNDNIPDGRDVSWIYDIDPTAIKDKLAMFNGLYISGTRCYDMAVRLQYAGCSIDMSKV
                     NPLIGEVVRQVLPKSKAEHVLVLPNYSAMLDFRKLLLGRKIL"
     gene            complement(26583..27986)
                     /gene="dnaA"
                     /locus_tag="PLF29_00140"
     CDS             complement(26583..27986)
                     /gene="dnaA"
                     /locus_tag="PLF29_00140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HXK52386.1"
                     /translation="MSQETLTEITLWKAVLAEMELNFSGMHYNTWLSRTEAVSLDEEK
                     VEIICESKLAKNKIETLYKAFILDSIKRICKKDLDLVVKIGQTKETRDQINTSPLFAT
                     TNKSDDKVSSLNLFKKAKENGLNPLFTFENYVMGNTNRLAYAIATTIADNPGTKHNPF
                     FLYSGVGLGKTHLIQAIANRALQNNPEAKIIYCTGEQFTNELIENIMKGKGGKGGYST
                     DTFRKKYRNADLLIIDDIQFIAGKDSTQEEFFHTFNDLQMKQKQIVLASDRPPEEYKN
                     IEERITSRFKSGIMADIQAPDYEMRTAILRNKRDRSGDKLPNEIIDFIAENVTSNIRE
                     LEGAYNQVIIFSKATGADYSIETAKQALGNNIKTEPSKPINLNQIAKAVCGYYAIKHN
                     DLKGKRRTKEIALPRQIAMYLMYELTGTPFMTIGEYLGGRDHTTIMHGVKKIESDIKE
                     STRMRQEVVTIKKALYQ"
     gene            28153..28305
                     /gene="rpmH"
                     /locus_tag="PLF29_00145"
     CDS             28153..28305
                     /gene="rpmH"
                     /locus_tag="PLF29_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018944472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HXK52387.1"
                     /translation="MPKRTWQPKKRKRVKKHGFLKRMSSKGGRRVLKRRRLKGRKLLA
                     RVLKIK"
     gene            28360..28728
                     /gene="rnpA"
                     /locus_tag="PLF29_00150"
     CDS             28360..28728
                     /gene="rnpA"
                     /locus_tag="PLF29_00150"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HXK52388.1"
                     /translation="MLPKQNRLSTRHQFKKVRYLSKKYNQEIVSPYFYLSYSYTDPEA
                     PTEVGFVIGTKLLKSAVARNRLKRVFRGCVRERLSDIKNGYWIVIIPRKASIEAPYEK
                     ISADINKAIQNICISGQTRN"
     gene            28658..28873
                     /gene="yidD"
                     /locus_tag="PLF29_00155"
     CDS             28658..28873
                     /gene="yidD"
                     /locus_tag="PLF29_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015395865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HXK52389.1"
                     /translation="MKKLALTLIRLYKTFVSPGRLGIKVCRFEPSCSRYTYEAIQKYG
                     ILKGALLGLYRFLRCNPFNKGGYDPVK"
     gene            28879..29655
                     /locus_tag="PLF29_00160"
     CDS             28879..29655
                     /locus_tag="PLF29_00160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HXK52390.1"
                     /translation="MELIKEFFNLILTVPLYNLTMFLYFLTGNLGLSIILLTVLIKAI
                     LVPATNPQLRTMKKQQALKPELDKIKKKYKDDKKKQAEAQMTLFKEHGLNPGSGCITM
                     FATIVVMLALYTVIRKIALATDIDQINQTLYFEALKVTEGIKTSFLWFDLSKPDPFYV
                     LAVVSGIVQFIVSKMTMSFNKSGEAAAKETPDRKDDIAYNIQQQMLYTMPIFNVVIGI
                     SLPAGVVLYLVVSTVFSAFQTYFVLREDISTMPVLKKEKK"
     gene            29652..30098
                     /locus_tag="PLF29_00165"
     CDS             29652..30098
                     /locus_tag="PLF29_00165"
                     /inference="COORDINATES: protein motif:HMM:NF013583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HXK52391.1"
                     /translation="MNKDQFITQYMKDVLNHLNVATVVNIDVAEDSVKVVLTGDDLNF
                     LIGFRGTSLDALQALLALSLYNKYEEWFNVFVDINGYKEKRFERLEDLAKKAIDKVRF
                     FSKEVALPPMNSFERKYIHEFVSSYDDMQTESTGEGSERHIVVSSS"
     gene            30198..31328
                     /gene="dnaN"
                     /locus_tag="PLF29_00170"
     CDS             30198..31328
                     /gene="dnaN"
                     /locus_tag="PLF29_00170"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HXK52392.1"
                     /translation="MKFTCLRENLAKSLSVVSKAIPIKSTFPILSNVLISTHNGRIKL
                     SATNTSTSITTYVGASIEEEGTITIPAKMLAEFISNLSSETVSAVLENNVLQVNAGRT
                     KSKFNGVPSDDFPPLPELKNEYLSMELDQKVFSNAVMQVGFSVALDDSRPVFTGIMMS
                     YSDGKLTLVSTDGYRLSERIVSLEPVLAKESREFSVVIPAKTLVEVARTFASSEENIK
                     VYVDEDESLCIFESGDTVISTRLLESSFPDYKAAIPQAGVVTANFASSDLLEAVKLTS
                     VFVKGGSSGALKIAVDPETSLLSVSSAEAEFGHNDTSIPAEIQGESIELFFNFKYLLD
                     FLNNIKYEKLTLVATGPDSACVLRSDEDSEFIYVMVPLQLNS"
     gene            complement(31399..33471)
                     /locus_tag="PLF29_00175"
     CDS             complement(31399..33471)
                     /locus_tag="PLF29_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HXK52393.1"
                     /translation="MPKLMLFKKKNKKQNKAQAEDVQQVQDQENPATRLADGMINVKD
                     IIAPPALEVDFSSIKIGNKYYRTLFVSGYPRFVGANWMSPVINFEHSLDISMFYYPVE
                     SKGVLDDLRRKITEMEATVMTNKEKGRLNDPGVEIALQDAKGLQEQLVKGAEKFFQFS
                     FYITIPADSLDELNNITKRLEATLGSLLLISKTASLQMEEAFQSTVPIALDKLMITRN
                     MDTTSLATTFPFTSSDLTSDEGIMYGINRHNGSLVIFDRFSLENANSVVFAKSGAGKS
                     YFVKLEALRLMIFGAEVMIIDPEEEYRMLSDAVGGNYISFSSSSPSKINPFDLAGVST
                     GGENELGQKILSLHTLFKLMLGTVSPIEEALLDRALMETYRIKGITTDPATQRDKEPP
                     IMEDLYKVLMGAAEPEARGMADRLEKYIKGSLVGIFDSQSTVNLNSKLTVFSTRNLED
                     VLRPIAYYIILDFVWTRIKKDLKKRILIVEEAWSLLQNEDSARFIYGVAKRARKYYLG
                     LTTISQDVEDFLNSEYGRAIVTNSSIQILLKQHPAAIDKVAETFYLSEGEKRLLLSAD
                     KGEGLFFAGANHVAIKIKASTAENALITTNPEEILRMRREAEAEAGITTKTQMPQMNR
                     SVELKASSDQSSFVSKDAATEYENTLNPIQESEAGEGLNLENIETDINNEPADENLPD
                     PSAPPAQF"
     gene            complement(33473..34171)
                     /locus_tag="PLF29_00180"
     CDS             complement(33473..34171)
                     /locus_tag="PLF29_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52394.1"
                     /translation="MSDIKATTQEHLDIETIKDDFVVLKNGSVCSVLQTTAVNFDLLS
                     EIEQDAMISAFSMLLNSFSFPIQIVIRSKRLDITKYLESLERNEEKVSDPLLKSQAIQ
                     YRQFVQEVIEKNDVLDKKFYVVIPTGGIVDLKKGTNPFEWFFVLFGFHSKRAPVNVDK
                     ALTKAKTELTPKVAHVISEFNRIGVKATVMTTQELVELYFDIYNPSTIHGQRIRTNIE
                     DYKTAIVNPAIIEE"
     gene            complement(34224..36188)
                     /locus_tag="PLF29_00185"
     CDS             complement(34224..36188)
                     /locus_tag="PLF29_00185"
                     /inference="COORDINATES: protein motif:HMM:NF024078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HXK52395.1"
                     /translation="MPLNDTQTTNKQHALPQNIMDVEFKIIGDLTMRQFMYLALSGGI
                     AYGVFAFVNSIFKWPIILFLVLFGLSLAFLPIEDRGLDQWVVNFIKSIYLPNQRIWKK
                     TPEIPAAFTFENLNVVRQELITLSPTTSRRKLEEFLTAQDSAPNNDPLDFQEHVYINK
                     VAAAFAGTPVSKANPYTPGGNVQKRMGPGIQTAATAPIAPAAHAKVIDDAGFQKSHSP
                     SEEKRLDSNQYSAAVPGNIAGAPGAGSLSFGSAPKTIEGSGGQAPAGAGASQTTQAAT
                     AEPKHLIPEAQFDNAYNPSEKPQFLTTSYFEPAPITPDRHSGRAFTNLVPSKGEIVLP
                     IKGEKTLRTSEEMEIDEELQNKTKQLNALLEQIKRDAKYKNIVAAKPVEKEIPLTSNV
                     QGSLQMNHSALEEKSSFPSNHEANNASTINTTSTNTGYSAQFGPDDEIITTTQSPFGM
                     PVDNTQPVIQIGQDIKNLLAKLQRENKELSIQIERLKQDIANSKGDNDKAEKITTIQK
                     LEKEKEATEKNYSQIKEKYTELTEKSFVKKAIEEDRERTEKVIHGETPEFIESGISNT
                     IPNIISGKVVDQNNRALENIVLIIKNHAQEPVRALKTNPLGEFAITTPLLNGTYTIET
                     DKSKRTNLSFDIIKVEVAGKIIPPILIKGR"
     gene            complement(36347..37705)
                     /locus_tag="PLF29_00190"
     CDS             complement(36347..37705)
                     /locus_tag="PLF29_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52396.1"
                     /translation="MAQFTNTKNFKLLSFLLTLTVLFGTVASRSIYAANCDPELDTFE
                     NVGKSSAIDIRMPTVGSMYAAGLTGASSIAFNGNATQVTQCGGYALSEYSAQGVDLQM
                     DAEDCQPQDPTYCEGLGNNFSEPTTSNGGDFDARGKGSLLGLANAFQEANTEPMPVNM
                     AYFIQRETEGIPLISPVFAQQTGLQYTSPWLDIVYEFWAMSRNVAFGVLAIVSIVVGV
                     MIINRKKVSAQVAVTVQYALPRLILAIVLIYFSYPIGAAIANLAYVAKYSVVGVIRDI
                     AQTTSANTLTANGVVAAANIGFGLEIFILAMLIFQAFIPAAGVAFIVLFTILIMIVIL
                     YLIVQVKLFIFYIKMLIEVIKAPLIFVFGAIPGNEKATVGWFKTMAGYALGILAGQAT
                     IAITRVASITIMGTSSTGLIPMPYFTKGALTLIIYLVGYNYARKAPDKVKALVAGDDK
                     KR"
     gene            37767..41321
                     /locus_tag="PLF29_00195"
     CDS             37767..41321
                     /locus_tag="PLF29_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52397.1"
                     /translation="MASSNPFYVGYHPDDKPTQIKPKNILQGADPQKALDSLLNTGLL
                     DQALQDIDVPRYVKEKIKNDFLSSLAVQIKDSSVSAADLALLPPEALQDMQGSFGIKI
                     SLNPLDWKDDPVKQLKKTLSSWVKAAYDPDDFRTTTMMKVAQSVYGNSKPYTRTSSGA
                     SRRYIISDISDRLADLQHKIDESAISSAALRDSARAGRNREMQAAVAAGDPNPLKIVG
                     YVTKTERKSITVKDASGADVTISADKVSDPYSDVVQSYISFLQSSSSGTSKREGGMDG
                     LISSGVGAILSEIDVDIEGLSLAPGVTDYSDKVRALIDTRFAGQPAAVRDTYVSQILS
                     LHSQQELAKSMYGFNEFLSNANPAKSLGISAALDQYMAVGRSQRQALAADPAFMARFT
                     NYSAEAAKARTALENFKLSPEYLRDPDKYKGIVKSFERNFKQLDSRVAPLVTAFQANP
                     SVNSARALATASKRITAVGLTAPIAGGDHLQRAPLRKLYRQMYEDHLRPQGPGIINSG
                     LLQRSLGLGVLFNEAEKIRNENWAEDVLQLLEGGKLTDTFVWMRVKNTFPYVFPSYWV
                     GRVLDKVQYFGLVIDESSIEKTTTTLFGIRRFYKDLLENSDGGGVLGFLFVNKVKVEA
                     TGFFGDIVKLNLTGGAHMNGALAMSKWLISNAGSANISDIDDMVKGMLTNFRFHYLAD
                     GSINIANTKSALTDFLLRDPKYKNLLPTLFKDGADLDDFLGKFIVFKQYVDAKLAKYL
                     GISIDMLDQGLGLEFVVFQELHKFNLSNDTRGKLAVKLMGPLQKIALKLNILQTNILN
                     KFPITRLIFGGQNALLGLLLGGTYALKSNVAAFIKSGLAKLFGKFGGGAVSGFFAWLG
                     PLGAMLGKFADKLLTILIRKGLDMLEGLTKAIIHLDISYFTKSYSKFVDNTTKIIVYL
                     LAIPLLLMFLFAAMINTVFNSSTPPTSQAAVGQSTPLTGGCFGSGCGSGTGSTYECGI
                     SKTTGNTCIFKDGNYSVMNGSLNIGADSGHGSNSYWDWLVAKKVIGSGQKCSFTLPML
                     ITDGIGPTRITDSGSIPGSFYCRGDGTTTESPLYGYAVDYYTAPNCGSVFAPPINGNS
                     VTWNLTPYPTLTGAGDAVFLTTTQGGTTYRLTLLHVVNIVSATSVDTGDFIADMYNWQ
                     VSGSDNSHIHVEYAEVTGGAVTVMALDDPSTGSPAFCR"
     gene            41348..41845
                     /locus_tag="PLF29_00200"
     CDS             41348..41845
                     /locus_tag="PLF29_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52398.1"
                     /translation="MIEKIKAFYYRHKITIWVAGGVATIVFLIIVDNLVANLSTQRPS
                     VEPVYNTKQQDVKILKIFPPDGAKVSSPSNFEVISVTLSAPIKKGSLVIKINPYIGYT
                     ISYSDENPSILTIVPKGQGWAPNVTYTIDFLHALDDSGKKVYLNYPYVFLNTPPDPAS
                     IVFPF"
     gene            complement(41977..42453)
                     /locus_tag="PLF29_00205"
     CDS             complement(41977..42453)
                     /locus_tag="PLF29_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52399.1"
                     /translation="MQRQLFKKIVIFGSLLTMLLIAPQRTYAVTGVAGLGCDQLTSVD
                     APEFTQLMCPIIRVVYILLYASGAVFSMMVIITAYKYITAQGDPKGIMGANLSGTQAV
                     IGFLMIIGSFVVLRLIMNVTGLQASMNFFDDVIAGICGIFTDTNGNLIIRGLPGCQ"
     gene            complement(42434..42766)
                     /locus_tag="PLF29_00210"
     CDS             complement(42434..42766)
                     /locus_tag="PLF29_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52400.1"
                     /translation="MKNMFYYLQKINGSASGLTGSNFNSIGDVLGLILNVMIGVGISL
                     SIIFIGFSGIRVITSLADPKKYATAMKSFYYSLLGLLLTFAVLLIRIILLNIVGIGGD
                     LQNATPTF"
     gene            complement(42828..43193)
                     /locus_tag="PLF29_00215"
     CDS             complement(42828..43193)
                     /locus_tag="PLF29_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52401.1"
                     /translation="MLVEKMLKTAHAQTSVPVTVVDPFSGSPIATAGTLAAIFGVVMN
                     LVIGVGIALTVIFLGIAGIEHITAKGDTKKAASARTALTNALIGFLIVVGAITIKIIA
                     QNVVGISGTNSGISGITPF"
     gene            43504..44244
                     /locus_tag="PLF29_00220"
     CDS             43504..44244
                     /locus_tag="PLF29_00220"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HXK52402.1"
                     /translation="MSFFLALLVILVILIVPFLKVIPEGYHGVVLFLGKYNRTLNPGL
                     NFVLFPLEQVIKVDIREQVMDIPTQEAITKDNVSCMVDGIVRFKVLNPSTAIFKVQNY
                     QSQISAKAQTALRDVIGSITLDEVLTKRAEIAEHIKGIVDTAAEQWGVDISAIEMQNI
                     EIPADMKRAMAQQAEAERDKKARIIKAQGEKEAAVQLAEAAQVLSGAKGALELRTLQT
                     LKEISADPSEKIIMTIPNSFFEVLNPRK"
     gene            44252..44908
                     /locus_tag="PLF29_00225"
     CDS             44252..44908
                     /locus_tag="PLF29_00225"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HXK52403.1"
                     /translation="MNRGKIQIAFNYTFNDFAKVIRYIPKNPNIILEAGTPLIKYEGI
                     KVISKMRSLWSGEICADMKIADGAHEEVLMAKQAGATSVTVLGNSSVETLKIFVDSCT
                     KLNITSIVDMISNEKPLKTLWKANVVPEMVYIHRGRDEENSFGKILQYKDISMLKGKW
                     DLKAGAAGGMDKRELQSAIFNSADVVVVNIVRPSDSWKGIVFDSQFRTNLEDFLRFVA
                     "
     gene            45012..46790
                     /locus_tag="PLF29_00230"
     CDS             45012..46790
                     /locus_tag="PLF29_00230"
                     /inference="COORDINATES: protein motif:HMM:NF020075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HXK52404.1"
                     /translation="MQRPAIHARKLFLILLPFLLLTTVLSYSAFADSCKNIEDLDDRE
                     KCYAKEYESTEKKLQDVREQKDSVSKKISDLSSALTVKNEEIVELQNEIAAMNSVLNQ
                     INQTLEDKNLQMAKKIELRNGMLRTFAKKGNLSTTDFELIFGTAAVSENLTGFQYATL
                     NYIFEKSLNSDAIRTIKVLQTEIQNFERDKKEAEDLKLELESAQSNLLVLKNSLSSEK
                     ASAEGQYSELSDKEKSYSQKLSDISAKQQEILNLKAGGDNGTVGDYDSPSWKVPEPPF
                     SPAFGAFSYGAYTHYKGMSQYGAKGRAEAGQDYKDIIKFYYKQDVKEKDDFPSKICVQ
                     GYGDLDFQYYLYGLAEMPSDWPEDALKAQAIAARTYAYRYWKSGQCICTTQSCQVFLK
                     SKADNVPSRWKEAVDDTKKKIIDGDTTAMYASTSGGYVEPGGWDTSGSWPGGAYEKKA
                     KSPWFYWAWYSQNYRFDSSTCGRSHPWLTEKEMADILNSWVVWDKGSNSDRDRISPVT
                     TGCWGGNPYSLDQMASKADDYGTKYSKINSVDVTVGNNGVTTQVKFSTDKGNVTISGD
                     VFKTVFNLRAPGYISLRSRLYDLQRE"
     gene            complement(47034..48269)
                     /locus_tag="PLF29_00235"
     CDS             complement(47034..48269)
                     /locus_tag="PLF29_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52405.1"
                     /translation="MNRIKIIFFYTFVAGVIWAVSAVGIPIDNDVLTRAFVKTAHAEG
                     THEPTNYEFLMDSFSSEVANCGSMALNSIVNATVTINPKIPASTDPSGYYSFVHVDTV
                     RFNLHVYDQNGVRIGVSNEIEWPADSSGYVFQCQEDGAISGPGCEPSSSTRLFGTGVE
                     ISDPATSQVIVQVNLVEVAGNYNWVSNDSNVVEAARFSINLSQPFSAPTTCYLASTAP
                     ACDATFIANHNGRDLCGPVKVACTTGEYCGYTGVPPRPGCVLDVDGDGNGFTDICGIP
                     TGILPSGECTYDPAYINTCGYGGCAANETCNTSGQCVRNITCIPAPPRREYTGPLINF
                     ETLLTSLMSLMIPAALGFFSLPMILINGYKTMTSQGDPAKVKDAREGFVAAVLGTIAL
                     LGFIVIIRLAIQSLLGLTI"
     gene            48511..48933
                     /locus_tag="PLF29_00240"
     CDS             48511..48933
                     /locus_tag="PLF29_00240"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HXK52406.1"
                     /translation="MQVFLNTDGGARGNPGHGAAGIVIFENDAVKHTRKKYLGICTNN
                     EAEYLALILGLEEAIKHGYKNIVCRLDSELVVKQLTGVYKVKTPHIKILHDKAATLVK
                     KFSTVIFTHVPRSQNSGADSLVNEAIDEYLSSEVRKSY"
     gene            48943..49833
                     /locus_tag="PLF29_00245"
     CDS             48943..49833
                     /locus_tag="PLF29_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52407.1"
                     /translation="MSYRLPRNATALEQAIINELKYRAVFNASLTYYQLWNSLFLLHP
                     INESQQKEFTLENFNKVLKKLKQSKYINRTTTGNYYIQTKKPFKWGHRANYAQEYLNS
                     LGPVVKVLSHIGWIKLLAVSGSVAAYSAKKNDDVDLFIVTEKKRLWLTRLFVVLLLKT
                     LRSYRTDKNSANKICPNIFLDEGALTWPTERQNIYVGHEIILLRPLINRDNTYFKFLD
                     ANSWIKGFFCTFCYASEKTYKSIYKPGSKLVDLLEDFVKMVQLRYMKQKITSEIIQDR
                     LLHFNKHDHTSKIINEFEKN"
     gene            complement(50355..52007)
                     /gene="groL"
                     /locus_tag="PLF29_00250"
     CDS             complement(50355..52007)
                     /gene="groL"
                     /locus_tag="PLF29_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007932107.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HXK52408.1"
                     /translation="MSAKQIIYGNDARTKLKAGVDALAQAVATTLGPKGRNVALDKSW
                     GSPQVVHDGVTVAKEIELQDKFENMGAQLVKEASSKTNDIAGDGTTTATVIAQALVEE
                     GIRNVSAGANPMILRKGLEKATDVVVKEIASLAKKITTKEEKAQVATISAQSSEIGNL
                     IADAMDKVGDNGVITVEESKGFEMELDHKEGMQFDKGYASPYFVTDTDKMTAEINDAY
                     LLVTDAKVSSMQDLLPMLENFVKVSKNLVIIADDIDGEALATLVVNKIRGTFNALAVK
                     APGFGDRRKAMLEDIAALTGATMISQDMGRKLDSVTVEDLGRAERVFATKDETTIVGG
                     TGNEVAIKERVQQIESQIKNTTSDYDKEKLQERLAKLTGGVAVIKVGAASETELKEKK
                     LRVEDAVNATKAAIEEGIVPGGGVTLLKIRKALLDMKLEEDEEVGAKTLYNALEKPVR
                     LIVSNAGVDAGRIIGEIERLATEKKNSNMGFNVMTMKYVDMIEEGITDPAKVTRSAIQ
                     NAVSVATMILTTECLVTDAPQEKDENSSAGGMGGMGGMPGMM"
     gene            complement(52045..52341)
                     /locus_tag="PLF29_00255"
     CDS             complement(52045..52341)
                     /locus_tag="PLF29_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HXK52409.1"
                     /translation="MADTKIKPMFDYILIEPLEEETTTPSGIVIPDTAKEKPQKGRVI
                     AAGAGKRGENGDLIKMEVEVGAIVMYKKWGGTEVKVNGKEHLLVKQDDVLAVVE"
     gene            complement(52512..54335)
                     /locus_tag="PLF29_00260"
     CDS             complement(52512..54335)
                     /locus_tag="PLF29_00260"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF002436.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HXK52410.1"
                     /translation="MTSKEILEKYIQFYKDRGHELIPNVSIVPENDPTLLYVNSGMFP
                     LVPYLSGQSHPMGKRLTNVQRCLRFFEDIENIGHTNRHTTLFHMVGNWSLGDYFKQEQ
                     LPWAYEFLIEVLGLDPNKIYATVFKGDEDAPKDTEAVEILKRIFEKYGIEAKEDERIF
                     AYGKKDNWWQRGEAVGELGGPDSEIFYYIGREGTGFGLSPHENEDEFLEIGNSVFMQY
                     KKAEDSWVELPQKNVDFGGGLERLALVAQEKNDIFETDNFWPIISELERITGKPYKLD
                     NNTTIAMRKIADHMRAATILMMDGVLPSNKDQGYILRRLIRLMVRAGRFLDIDQDISS
                     KLLSPTINMLSWIYPELPKIEGEALKILRDEETKFRKTLHKGSKEAEKELSGIGGTTT
                     TQHLAEISFALYQSVGYPQEIFIEDAKEKGVNLDQKDFEEEFKRIQQEHQALSRVGAE
                     KKFKGGLAEHTEQITKYHTATHLLHWALRQTLGPQITQQGSNITNERLRFDFSHNQKL
                     SEKELKAVEELVNKKIEEAIKVQYEIMPKETAQKTGALHFFGEKYGDMVKVYFIGDSL
                     ESAFSREFCGGPHVQNTKEIGRIEIYKQEKIGGDKIRIYAK"
     gene            complement(54343..54416)
                     /locus_tag="PLF29_00265"
     tRNA            complement(54343..54416)
                     /locus_tag="PLF29_00265"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54382..54384),aa:Cys,seq:gca)
     gene            54484..55743
                     /locus_tag="PLF29_00270"
     CDS             54484..55743
                     /locus_tag="PLF29_00270"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HXK52411.1"
                     /translation="MSISTLVPTKERKTSKPFYTIILYILISFGFVALAYFGLVFVKN
                     ISKSKIPAALELKVENGEAEVFLDGVSIGKSPIENYQLEAGSHEVMLQSGDGMSYKTN
                     IDFVPGAAAVVIKELGSSSLFSSGASVWFAKKSADSSLSVISEPSGADVYIDGTLVGS
                     TPYNSAKLSAGGYEIKVAMAGYEDRVLRAEVLETHTTNFNVILFPNAVPSEVGTIEGY
                     DNIYDVTSNDPRVLSDISSWIKAINYWTTTRGVNIKDLGLTKEPLFTYFIDAEGIVYG
                     TDGTTLSADQVAGLSSDQKGAYLHSKNVSETEASQLTEKAKSSVEKLGTGPVLTEAEN
                     KEIPGTENAGTVGTPQTGIGGQATILDTGLGWLRVRNAPGLSGEEIAKVNVGEAFEVL
                     DIQGDWVKIRVDANVAGWVSATYVSID"
     gene            complement(55876..57933)
                     /locus_tag="PLF29_00275"
     CDS             complement(55876..57933)
                     /locus_tag="PLF29_00275"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HXK52412.1"
                     /translation="MSTTSSIKLIILLLPIILGLPGQETYEFSKFIFTLIAVSLAFMA
                     QKQNNRSSADRLSYKSGFSETDALVIGNFILLALSTLASTYFLGSLIGYYTRYGDSFL
                     FQIILLATYLLIRKIDTKKSQELIETIAFSTLSVSIFALVQYISTQTFRIGGTFGQPN
                     WLGSYLATTILLNLALLIQTNKEQIKGRVFWYNLNAILGFSVIVLTYSLSALAGLFIG
                     LLVLIFFGAKLGSSRKNIHKTTNIIIAKCKKLRITLIFFFVFSTVCAAPLAKRVHDVF
                     EDIKTSFSGTNYALQNPQYSNRATNNIKSNAPDTFPNTNQRFYSDPAFIRGQVWLGTI
                     QLATSSPARFILGYGPETFPFVFQNFRPPALNFSSEWDYIINKPHNYYLEMLAEQGIL
                     GLIGYILLVSYLYRRKDEILSPALSTIVITNFFGWPSIALSFIFIVIVGIYALKETRE
                     GEDELNNYGKLLMKFEQKTQNSRETLARIKSLNRILNGPVHKLSKLCTLGIFVLIIVF
                     ALRYAAAEYFYSKARNAILNGSVSASNENIDVALRLWKYEPNYYLQKAKALTLQTLDT
                     KPNMHAQLKKQAIANIEKGIKINPNNLATKRDALGLYYFLAINDLAMPSSKDNIDNAT
                     IGDFLDYSEKLQTDYPTDLGVQIQAAKYQNLLGLEEKFKESLVRIETLRPDILQWNSD
                     LLK"
     gene            57971..58537
                     /locus_tag="PLF29_00280"
     CDS             57971..58537
                     /locus_tag="PLF29_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52413.1"
                     /translation="MFSKILIRLVDQAIVPAILLLVSRVTSMLLLSAIFNVPYELTNK
                     GFTFLEVSDYVRINSYSMLFMLVCVFVGLLYIMLKSFIFHDTHINPVLAARLFTLRLD
                     SFIQASFDLYSQGAVWLSYLYLLTVVIIAMVFTGMLYSWIFVVAFIMSIVATVLLVFD
                     IENELEYTKDSKEEQADSLDEYVLEMEV"
     gene            58539..58904
                     /locus_tag="PLF29_00285"
     CDS             58539..58904
                     /locus_tag="PLF29_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52414.1"
                     /translation="MDEYQGFKSKHLLRGLTDGLLIAMFLGLLAMPISAIGLFAYQNS
                     SFSSVASNTKSQDDILSQQPVIEPASPSVAVLGAQSDPLLTDLTLEEQKLVEAYIEYL
                     KLSRPRGGSVLDLQNIPSN"
     gene            complement(58980..59945)
                     /locus_tag="PLF29_00290"
     CDS             complement(58980..59945)
                     /locus_tag="PLF29_00290"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HXK52415.1"
                     /translation="MVGFIYHVIRTARPRQWLKNLSLFAAPVFAGTLFETNVFANTVK
                     GVVVFCILSSGIYFFNDLMDAPKDRLHPIKRNRAIASGKISAKQAQIIIFILLGGSVL
                     GGYFFVGTYFTLAVIFYIAIQVAYSIKIRNIIILEALFVAGGFIIRVFAGGFAAESSV
                     SSWLILTTTGLSLLLAFGKRRSEKTILLKHLDTKDQIKTRDTLRHYPDNLLNSMISMS
                     STFCIITYALFAFQISPETNDAAITKVLPSIISSPKWMMLTIPIVIYGVARYLYVIYE
                     KEEGESPARVLMSDKPLFASVITWGVATLFVIYLLPSILIYFSTL"
     gene            complement(59890..60738)
                     /locus_tag="PLF29_00295"
     CDS             complement(59890..60738)
                     /locus_tag="PLF29_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HXK52416.1"
                     /translation="MENLYNLIFPKSCVLCKTRHAVLKKVYGPKFVLKDAIAKSENAL
                     LCSDCLAWFKVLNNSNCIVCDKPTIDGKTHKLCASEYSPEATLGIYEYAGFVRLCIKE
                     AKFGSKHFEILKEISTNAANNLLQSGVRDLFEYNSAPIIVPVPPSKSKLKKRGFNQAE
                     LIAEIFAKHFKLEINSGLLKRNIDTYHQYGLTRRNRYKNVQDAFSAKADSRGKEIILV
                     DDIWTTGATLLETAKAMKKSGATKVYCLALSKKPLKIRAKRHTIYSNGRVYLSRNQNS
                     KATTMA"
BASE COUNT        18032 a        12175 c        11753 g        19051 t
ORIGIN      
        1 cctcaagaat aacttggtgc aaatctggtg gactgtagcg gaatcgaacc gctggcctct
       61 tcaatgcgaa tgaagcgctc taccaactga gctaacagcc cgtacactgc acattatacc
      121 aataattcaa aaaaacgtgc tgtttagata agggacattc ttcaaggcgt acatctaaga
      181 atgatctttt gtcgggtggg tgtgtcccag ctctactcta tttttgagat actcgatatt
      241 tttttctcgt tgttttagta aatcgtcaac ccctgcccag tgcttgccgg caagcatcga
      301 agagaaatgt ccgccaataa aatgcggcaa aattacataa gccgctcctt tatcgtaaag
      361 ctgaagagcc tccgcgaggt tatgtgacat tactatggcc gcaatactag gatttataga
      421 tattgcctta gatatcagaa atgtgtttgt ctcataatcc ggaatggtcg agattatcat
      481 ttttacattt tgtaaattca aggcttcaat aagctccgcg tcttcggcat cgccataaat
      541 acaattaaat ccttgggcat tcagttgttt tattattaag ggatcaaaat caactgctaa
      601 aaatccgttg ccaagttctt taaatagctt taggaaatcg tacccgaccc tgttacaacc
      661 aaaaagaaca acctcgggag aagaaatttt ataatgttcc gattttgaag atttccgctc
      721 aaagataacg agatattttg ctaattttgc gtaaatagtt tcggaatata taatcaaata
      781 agttgagcct gcaatagtaa ttagacccac taccgtaact agcgagagta ttttctggct
      841 taaatgtccg gcctggactc caagtaaaat caagatcagg gagaactcac ttatctgagc
      901 caccgtaagt cctgccataa atcctgtttt tttgttataa cgcagaagcc ccattaacac
      961 cataacgatt accggattac caataagaac aaaggccgaa aaaactaggg cttgaggaag
     1021 aattgtagca atactatcaa gactcatttt catccctaat aaaacaaaaa acataattat
     1081 aaaaaaatca cgtagaggct tgagcttaga tcctatttcg tggttatagg gagacatcga
     1141 cagtgtgaca ccagctatca aagctcctat ttcaatagaa aagcccatat aggcaaaaat
     1201 gtttgctaaa cccagccccc acccaattgc aaaaagaaat aaaaattcct gtgatttagc
     1261 aaaaaatccg cttagtgtag gtagaatctt aatgcttatg aaggaaagta tcgctgttaa
     1321 aactgcgcct ttcaggattg tgaaaagaat aagagtataa gcgttcatgt ctcccttcga
     1381 ggcggtggaa acgacaacca gaataattgt tgcgataatg tcctgaacga gcaaaaaacc
     1441 tattgagatt ctgccgtaaa gcttttcgac atctttttta tctgttaaca actttaaaat
     1501 tatgattgtg ctactaaacg tgagcgcaac ggctataaaa agggtttcaa ccaaactgta
     1561 cccaaaagct aaagctatga gggaaccgaa aaaagtggta aaaagaactt gcccgactcc
     1621 ggttattaca gcaatctttc caacttcgcg cacaatttta gggcttaaac ccaagcctac
     1681 aataaacaac agcactgcaa ttccgatttg cgaaaaagtc tcaatagttt ctgaggaatg
     1741 ggttaagttt aaaaaatatg gcccagcaac caaccccgta agaatatggc ccatgatcaa
     1801 gggctgcttc agtaactgca ttataccggc catagtcata gctataaaaa taataatgct
     1861 gatctctgta aaaacgttca taggcagtgc ttcaatgata gcacgaggga atatgtgttt
     1921 ctacctgata tacattccac ggacttctcg aacagaatac cctaaaaaat tgatacaact
     1981 aatgggaatt tttataccac ggatagttct gggggcgggg tcctcagtaa atttttttac
     2041 ttaagacaag cttctctttt cctgggggaa gggttcttgg gtactcgaat tggcgcccca
     2101 gtaatataaa acgcagtact gaaaaaaaca agctggccca agaaagccga agaaatgtaa
     2161 taaaaacaca gcaacactat agtaaaaata gcactgagcg ggcactatag gacagtaaag
     2221 attctatgcg ggctattcct cagtattctt tacaataatg gcttcgcgta aacgtacaaa
     2281 gcccgtagtc tgttcccacc attgaaaaaa atcagttttt ttactttcct gtaattttga
     2341 ggggtcgttg ggatcataat aaacataggc ttcatcttcc tgcctaacaa taacaacata
     2401 atgattggct cccactaaac atatcacgaa ggcgccgcca gtcaaagcaa gtttaaaagt
     2461 tatttcagga tcttcatccg tttcccaaag actgaaagaa tgttcatata ctagcgaatt
     2521 agcacattct tttaaaccag aggcggtcac caaaacacca tcagagccgt ctaattccct
     2581 agcaacatct tccaacttgt tgctgtcaaa gtggaggaat tcctcccagt ctacttgtgt
     2641 tctatattca cactgtaacc cctgcgtcgc tagaaacttc aaaattttat cgggcaaagt
     2701 acccagatct ttttctttgc caatctcagc acgcatatcg tttaaaggcc tgtactgtat
     2761 tcctctaaaa ttatgagcac aaaagaaagc ggcaaaaaca ccacaatctt taagccccct
     2821 attcgacgcc gtagggagaa aatactcacc ttcaattctt tttcggcctt ccgacccatg
     2881 tgtaatttct tctttaatag acatgcccta atttacacgg ttttatgcaa cagtggtgtg
     2941 cccggaggga ctcgaacccc cgacttcagc gtccgcaacg ctgcgctcta tcctctgagc
     3001 tacgggcaca taaaaccttt tcgatacttg ggattatagt agagtaagag caaaattgct
     3061 acagtttgcg actttttact taaaaaggcg gagtcaacaa ctactcgact tacacattaa
     3121 gcttgcttat caattctcga tatgccttat ccaaaaagtt ttccggcatt tcttccaaca
     3181 tgtccatatg tacagaaagt aattggatta actcctgctc atcaattttg ctaccggtta
     3241 aagtgaggta gattcttgga ggaaaagctt cgtaagcatc aatacctaga actctaagat
     3301 cgtcgcggtc aaaaataaag aaatgcttta gttcatccca aaaatagaac tttccctcta
     3361 tctctaggcc ttgatttgtt attttatata aaacggtttc gggagcattt tttgtaagag
     3421 cataagatag tatgtatata cccagtatca aagctattaa tgtaaattct tttgccgcta
     3481 taagaagtgc agcaaaaaac acacctatta ccataagagt tcgtaaaaac ttttggctga
     3541 gtggctttct gtgaggcctt gctaaagcct cccattcata tatagttttt ctgtctgatt
     3601 tttttggtcg tgctttttct tttagctctt gctctctttg atagatgttt tcttcttttc
     3661 tagtggtgaa acccataacc tcactaagat attttaggtt taaattcata ctacaatgat
     3721 atagtttttt tagtctttat tcaacaacac tccataaaaa agttttgagt tggtgtgttt
     3781 ctaacaaaac gagctatttt ttcgtggagt atttctgatc tggttgtttt ggcaggaaaa
     3841 gatggtggcc gcggataaat ccgcggccac taaatcaaaa aaacacaaag taaactgctc
     3901 taagctttag gctcagactc agccgtcgcc tgtggcgcac tattgctaac aacaggggct
     3961 gcgggtgcct cgccgtaagc attggggcct gcatcgcttg gcaaaacagt aaatacaaaa
     4021 actatgatac cgccaataaa aggtatgagg cttagaagca acaaccagcc ggatttattt
     4081 gtatcatgta gccttctaac agaaacggct atagacggta taagtacggc aaacatatat
     4141 acgtagtaga ctatggaaaa aattgctcca agatttgtgt ttatattaga agaaactccc
     4201 attacaaaag caagggcaaa ataaattccg acgttgatta gctggaacat ccagtattca
     4261 ctacggccgg cccgcccgct aaaatcagcg tattttttta aaacagttat gtaccaatcc
     4321 atggtaatct ccttataaat aaatatatat ctacgataag tagacaacat aataatacag
     4381 cagttgcatt cctagggcaa tctgggataa aggaaaggta aatctttcaa atagtcgggg
     4441 tgcgctaaat ttgctgttta aaagtctgca acctaagcct aagaactaaa atgagaacat
     4501 ttagtaataa cataaccaag gaaactactc tgaataaaac atcgggaagt acaaaaaggc
     4561 tatataaaaa ggtgacaata gccgacaaag tccagacttg atatgacctt gagttggcgg
     4621 agggtttttt gtgaaaccac aaatctttaa ttgtaggcca atacgcaatc gcatcaacta
     4681 aagaagttgc gccataagca aaaataataa atttcaaaat aatttcatgc atatataccg
     4741 gaaaggcagc gttttacaca aatcaaaatt caaaaaaacc tccgaacaca aaagcttttc
     4801 tagagaatat ccctacaaaa cgacatttca gcctaagcat cagcggcgtg ttttcgaaaa
     4861 ctccaacaga tttcgccaaa aactcgtaga gagtgcggta ttcgtgtcaa acccccgccc
     4921 cctcagctaa cctatagaaa caatcctttc aagacgaact atttcaagtt tttagtttct
     4981 cttttagcgt tgctcaacgc ttagcctagt tttgcggaaa attcttttct ttaacttttc
     5041 gagatacgca aagttaagct atatcaatat tttatcattt ttttaatttt cagagcgaaa
     5101 tatgagagag tgctgagctg aggcgcgaac cagatatact gtagttaggc gagggtgagc
     5161 gaggagagta gaatctcccc tactatccga gcgaaagcgc agcgtcccct tgagtcgcga
     5221 agaaataagc tacttacttt tttggcttgt aaatgatttt gaatgcaagt tcaaaaatga
     5281 acaaaaaacc tactgctttg agagccgagc tatcaacatc caacaagctt agtatgacga
     5341 aaactcccaa tgccatggca attgagagag agtgaatttg ttctagtgaa aatcgtttat
     5401 tattcataat ttacaagcct aattataatc tatttcttcg caatttcgcc taactaattc
     5461 ttatacgacg tatcttcacc aacataacta cagttcgtag actatacgaa actacagcag
     5521 tgtttataac tacataatac acttagatat tcatatgtta aacagcttta tgcaaacaga
     5581 attacaaaaa acagaaaggg agttaaaaat caataactat agctttaaaa cctctaaaag
     5641 ttgtctctat ggcttacgaa agtatttttc ttttaaagaa aacaatttag cggaatttga
     5701 actggatagc attaaaaatt ttcttctcca atgccaacaa aaacgtatat cgcctcaaag
     5761 cagaaattta tttctaaatg caattaaatt ttactaccgt aatgtagcaa agaacataca
     5821 aaaaatcgat attagatcgg ctaaagagcc taaaagccta cctgccgttt tgtctagaag
     5881 tgaggtaaaa agagtcttgg aatcccaaaa aacattaagc acaagcttct gctttcactt
     5941 gcttacgggg cagggttgag agtagcgaag tggttaactt gacagtacaa aatttagatc
     6001 ttgaagagct gacagtgcat gttaaacaag caaaaggcca aaaagataga ataagcgtaa
     6061 taccgaaagc cttagttacc ggtttggata atttaactac agcaaaaaag ccggggacat
     6121 tgtctttgaa agcgaacgtg ggggaaaatt atccaccaga acagcacaaa aagtttttaa
     6181 aaatgcttta cgagcatcta gtataaaaaa gaacgccact tttcattcac taacactctt
     6241 ttgcaacaca cttgcttgaa aatggtaccg atgtccgcta tgtacaaaac ttcttgggca
     6301 tcaaaatatc agaacaacac aaagatatac acaggtcacc aatccattac tcaaaaatat
     6361 taaaagtccg ctattataaa aatgggggaa accggccttt gactagactt tcgatagcgg
     6421 gacaaaataa aaaatccaat ttgtgcttgg aattggccgg taagctctaa cctcgagcga
     6481 gccaacaaag acattttcca actggcggag ggtgtgggat tcgaacccac ggacggtttt
     6541 atccgtcacg gcttagcaag ccgctgcatt agccactctg ccaaccctcc aaatgtgagg
     6601 ttattatact acacaatcat acaattttga catgagccaa aaacacaatg ttgtataata
     6661 acagcactat gacaagcgcc ccagtagaca ataacgtaaa tgcactaaaa acccaaaaca
     6721 tggttgagcg ggctcaaaaa acgcacattc ttcaaacaga tgtttgggga gattttaaaa
     6781 cagctatggg aacaaaggct ataagggtgg ccggtgtaca gtacacttta cacagattac
     6841 ctaaaactaa catagcctac gcttattgcc caaaagtaaa cccaaaaaat ataatttggg
     6901 aagaattaga aaagtcttta ataagtaaca ggtgtttcgc aataaaattt gatgttccga
     6961 atataatttc taccgccgct aattataata actcaaacac aaaagaattg gagatattta
     7021 cgtcaaaaaa agggtgtatc aaaacttata aaagtacaat tactcaaaac aacgttctac
     7081 taaacatcga ggaggaagaa aacaagcttc tgtcgaacat gcatccgaaa caccgatata
     7141 acattaagta cgcgcagaaa aaaggcgtgc aagtaagaat tacaccatat aataactatg
     7201 acgagcaggc taaaagagac tttgaaatct tctggaatct acagaaagaa actagagcca
     7261 ggcaaaactt tttaacacac tctaaaaact atttcgagaa ggctttcgaa acacttggta
     7321 aacaaaatgc agcttttttg attacagccg aatatagaga aacgcccctg acctcatgga
     7381 tggtttttat aaaagacgga acactttact acccttatgg cggctcctcc gaaaaataca
     7441 gaaactttca agcaagcaca ttaactggat gggaagctat tacgttgggt aaaaaattta
     7501 actgcgacct ttttgatatg tggggagcat gcaaagatct aacaaaagaa tctgatccgg
     7561 aagtaggatt tacaactttt aaaattaggt atggaggtac gtatgtaaga tttattgatt
     7621 cttatgatta tgttctcaac aaacctattt atcacgcttt taaaaacatt tatccaaaag
     7681 tacttggtgg tttaaaattt gcagctaaaa ttaaaaattt agggaaaaca tcataagtac
     7741 taaacaaact ttccggtaaa agcttaaaaa actatcctat ttcgtagtaa taactatcaa
     7801 actcttctcc cttgatcacc gaggtcgggg gtataccact taaagaataa cggcaacaca
     7861 caatacggaa cggtcgtttc tgttcgagca gctgtatata gattctgtct atcattgagg
     7921 ggaataaata catatagacc gcggttattt gagacaggtc ttcaagcagc ttggttgagg
     7981 gtctaaatat gtcctctcta tatacgcagt aagtgccgta tcctttatag cgaagcttta
     8041 gaaatatttt ggattttagg gcttggtaac gatctttttc aacacctatt gcccttttaa
     8101 ctccgtgctc tcggagagcc gacagtaaaa ctctcccatc accgctaccg aaatcaacga
     8161 aaacgtcatt tccgctaagc tgcaagactt ctagagctaa atcaatgttt ttcttatcta
     8221 gcggaagata cggcgcgtcg caaagatacg ttgtgtatac aaacgtgaac agctccaaaa
     8281 aaactaaaga tcctgcaaca acaagtatga aaaatagcaa cattaaaaat aataaaatta
     8341 acattagtaa gatactttct gcgcgtgagg gcaaatatct gagtaaggac acagcttaca
     8401 cacgtgcccc ggagtaggat caaacttatt gtcttgaatc ttagagaaca ctgctaaagc
     8461 ttttgttttt atggcctcga gctgatcgag tgttcgagtg gtagatactt tttcgttatg
     8521 ctcaacaaag tacagcgtta gtttttttgg caccagcttt agcacttctt tcgccgctaa
     8581 tgcataaata gttacctgct cattttcatc tacctctttt tgagacttac tttgcccggt
     8641 cttgtagtct attatttcga cttcgttgac accatcttct gagtccagcc tgtctattct
     8701 gtctattctt ccgtttagtg ttttatcttg atctaagctt agataaaaat ttttctccaa
     8761 tgcaaatgtt ttagcagaat ttaaaatatc aacttcaaaa tattttttta gaacgtcttc
     8821 tccttttttg taatatcctt cgcgctgttc tttactttga tatcctgtag gatcccagtt
     8881 agctttgtac atctgcaata cgtcttcaat ttttacagat ggcgaaacaa gttttttcat
     8941 gtgatagtcc cgtagggtgt tgtgaattgt gtttccaaaa cttaaactat attttgacgg
     9001 cgtaggaaca ttcaggacat gttcatatct gtacctaaac ggacacgaaa gataagactc
     9061 cattttagaa tagcttagta ccaatttctt ttcatttaga ttttttttgg gtttcggttt
     9121 gcgatagact gaatttttat cgaaaagggt gctctgactc tcggctccct cgaagtcttg
     9181 agaagataat tgtgtggttt ctatttttgt ttcagctaaa tagccgctgg gaacggtgtt
     9241 tctttttccg ccatagctct ttgcgtaagt taagtaaaga tatttttttg ccctagtcat
     9301 gccaacataa aacaatctcc tctcttcttg caaatgtgcg tctcctgagg gcaatctttc
     9361 tttcaccagg ctatcgggca aatcaattgc gtctgatcta ttacgtgtag ggaaccttcc
     9421 ggaaacaagg ttaatcataa acaccacggg atattccaga cctttactcg catgtgtagt
     9481 cataagctga acagtgtcgt agtcctgtat ttctgcctga gccgggctgt ccccggcctc
     9541 cagcatcaga tcaatatagt ttactaaatc aacaacagta gcgatttctc ttgtatctgc
     9601 gtagtgagaa agctcaaact tctttatttt ctctaaaaac aggtcgaggt ttttcaatct
     9661 tagctcattt tccagagatt cttcagacgt tagacgtttt atcataccta ccgcatgcac
     9721 aagttcatag gttagttggg agggcgtttg gctcgccaaa cgccctctat aatcttcaaa
     9781 tagctttaag aaactctgca cctcctccac cggggtactc actaacttat cccacagatc
     9841 aactctctcc ttcttcacct cagcaagaat ttttgaaata agatcagggg aaatattaaa
     9901 atgctgaagg ttcaaaatac gatacaggct gatagcatca tatggatcaa ctaaaattct
     9961 taacaaggca ataacatttc ttacttcatc ggtatcatat aaaccgcggt tacctaccaa
    10021 ctgatagggg aggccgtgtc tcctcagagc cataataaaa ggatcaaggt ggttatttgc
    10081 acgagcaagt attgcaaaat ctttgtatga atacgagggc tcttgtccca gtattaaaag
    10141 tattttcttt acaataaact cagcttcgct ctccaatgtg tctagctcaa taatctcagg
    10201 ctttatcgag ccggaaaaat cggccccaaa actactaacc aattctttag aaatattaag
    10261 ttttatttcg agagtatccg gattattttt ctgtatcaaa ctataagcag ggtccaatat
    10321 cgcttgggat gaacggtaat tagtaagcag agtcagcatt gttgtgtttg gataatcggc
    10381 cctaaaatct aaaatatttg aaactgcagc acctctaaat ttataaattg actgagaatc
    10441 atctccaact accattaggc ttctcttctc aacctccagt tccgcagaag gagagagaag
    10501 ttttattaat tcgtactgtg cataattcgt atcctgaaac tcatccacca aaacatgcgt
    10561 aaattgtttc ttatacctgt ttagaatatt attcctctct ttaaaaagtt taatggtcca
    10621 aacgataaga tctccaaagt ccatggcaga ctgctgcaac ttcaactcag tgtatttttt
    10681 ataaaaagag gagagctctt tccacctttt gatttcttca ggctcgccaa cataagactc
    10741 gacgtaactt tgcagatctt ccacactagt attttcatct tgcagtcttg aaacaaattt
    10801 taacaatgcg gaaattgctt ttgtggggtt actcaaaggg cgataataat ccagttcaaa
    10861 atcaaataaa tgtctacgaa ccaaaaccca ctgatctgaa taggataaaa ttttgtaaga
    10921 aggattcagc ccgatttcaa gaccttcaag tttgagcact cgatcacaaa aagagtgaaa
    10981 agtgtcaacc caaggctcgg tataacctaa aggcataaca gtgtctaatc tagccagcat
    11041 ttctgctgca gccttatctg taaaagttag tgctagtagt ttctcgggag gaaggttgtc
    11101 gtgctgaatc agttttttta tacgttcagt aataactcga gtctttccgg ttcctgcacc
    11161 ggcgaccaca agcaaggggc ccacagcatg atccacagcg gcttgttgct caggatttag
    11221 tacaaaggtt ccctttttca tttaacagtc attatacagc aaagcagtac cgaagaaaga
    11281 acgaacattc tattctaaaa ggttcttttg ctccaaacca agtttagagc tgtcttccgc
    11341 tggtttaacc tgctgtatgt tttcaagctt attaataatt ttttggtaat tggttttaac
    11401 tgtatccata gagttagtag ctcttctcaa atgaccgtct aaaaccccaa ggtcagaatc
    11461 gaatttttta gcttctactt taagtcctcc gagtgctttt agaattactc ctgcgtgttg
    11521 ctgtagctcc tgctgctgat aagcaaccaa aagtattttt aggaaatatg aaaatgtatt
    11581 tggggaagct aaaacaacat tcttagcttt tgcataatcc tctatctctg cggtattaac
    11641 aagaagctca taataaacat tttcagaagg aatatacatt actgcctggt ctgttgtatt
    11701 ttcggccggc aaaatatact ttgttgccac ctcgtcaatt ctctttttca catctctaat
    11761 aaacgtcttt ttcgcctttt ccctttcttc agggctatct tgagtctgca tcagtttaaa
    11821 gttttccatt gggaatttag aatctacagg aatcaaacct ttatcagtct tgatgattgc
    11881 gtcacaaaca tctccggttc taaatttata ttgagtttta tacaaatctt ttggcaaggc
    11941 attagccaaa acgtcatata aaaactgctc tccgagccct ccgcgaagct ttggggattt
    12001 tagaacatta ctaagatcct tcatgtcctt actaaattct tgaagccctc ctaggttttt
    12061 ttgtacatcg gaaataactt ttgtagcatt gtctagtctg tcccacatta gtttttgggc
    12121 tttttgcaaa gaatcttgct gattttttaa ctgaacattc agagattcac gctgatcttt
    12181 cagttgacta gctataacat ctgcattctt gtccaccgac cctttcatct ctttcaacca
    12241 ctcgatcaag gtcgtatctt cttttggtgc ctgtagttct tctttcagct tttttagcgc
    12301 tgtatttatg taataaataa ttcctaaagc caccagcgcc agcgcaaata ttattagtgt
    12361 agaagtactc atagggttat tgtaacaaga attctcataa atctctcgtt cttttaagca
    12421 gagagagtaa aggcgcgttt cctaaaaaac gttctctttc gtattaaggt ataatgttgg
    12481 tgtgcctaaa tttttatata cgctcacggt cgtaatattg gtaagctggc taaccttact
    12541 aacatacatg aaaactgttc caccaaaaaa tattcctgca gcactgcttt ggggcattct
    12601 cttcttcatc acaacctaca gcacaacctc agtgctggtt tatattttta ataaaggaaa
    12661 gaaatccgac aaaacagaca gggagttata cagacaaggc cttcgcgcct ccacagcaat
    12721 agcactcata ctaactttaa ccattactat aaagattgtg ttaaaactta tttaaacttc
    12781 tatctaacga gaggcacgat tcggcctcga gcttttctac gcttgctctt tgtattctaa
    12841 cctgcaacac atctgctata atccaaagca tggaaaaaag attttatgtt acaaccccaa
    12901 tatactatgc caacggaagt cctcatatcg ggcactttct gaccacgacg ataggggatg
    12961 ttttggcaag gtataacagg cttatcgtgg gggctgaaaa tgtgtatttt acaaccggga
    13021 tcgacgaaca cggcaccact gtctctgacg cagcaaaaaa agccgggtac gaagaaaata
    13081 ttcaagccta tgttgacgag gccgcacaag aatggaaagc tgtatttgat aagacgcaca
    13141 tttcatatga ttattttgta agaacaacaa gcccgtacca cgcaaaattc gttaaagaac
    13201 ttgtattaaa aatgcaaaaa gccggagata tttacaaggg aaattatgag ggcacttatt
    13261 gcaatgggtg tgaaaaacac ctgaccctat cggaccttaa tgaagaaggg tactgccctc
    13321 tacaccgacc ggaccaaacc ataaaaatta ctgaagaaaa ttattttttc cgcctatcaa
    13381 aatatgcgaa ccgcctagtt gaaatttttg aaaaagattc tgtagtaatc tttcctgaat
    13441 cgaaaaaaaa cgagatcctt tctcgcctta aaagcggggt agaagacttg agcataacac
    13501 ggccaaaaga aaaagtgtcg tggggaatag aatttcccgg ggattcggaa caaactttat
    13561 atgtttgggc cgacgcgtta tcaaactaca tatcttctct tgttataaat aacaattatg
    13621 attatttttg gggcaaatat acaacccact tagtaggaaa agatattaac tggtttcatt
    13681 ctgtaatatg gactgcgttt ttactttcag cgggctatcc tctttataaa aatatttttg
    13741 cgcacagttt tctaaatata ggcggcaaaa aaataagtaa atctttaggc aacctaataa
    13801 cccctgacga acttataaaa tattttggtg ttgatggggc acgttattta gttctaaaaa
    13861 acattcctta caaagacgac atagatgtaa ctatagaaac actaacacag caatataacg
    13921 ccgaccttgc aaacggccta ggcaacacag tggcgagagt cacaaaatta gcggaaaatt
    13981 cacaaatttc ttttgacaac atatcagagg ctcagtctag aaaggagata ttagaaacta
    14041 tacaggaaga tattgaacag gtaaaagtgc atgaggcttt gtccaaaata tggcttaaac
    14101 tatcggatct tgaaaagcat ataaatacaa acgagccgtg ggcgattgaa gataaaaatc
    14161 aattaaaaac tatcctgtcc cacgagctaa gggaactgta cagcattggc atactgctcg
    14221 agcctttctt acctgaaaca gcgaatcaaa tagtttcctc agtctcctcc tcaaagatta
    14281 tcaaagataa ggccttgttt gaaagaataa aatgatgtgc ttctgactta agtccgtagc
    14341 ctcttcttca ctgcttcttt gttacacttt cgccttcgaa tctaatttat aaatcttatc
    14401 tcagcaaata aaccaccacc ggcttacgca tatggttttc tgtaatttct ttgcattgca
    14461 aagcccggca tgttgggtaa actacgtcta ctggcccacg caagcagttt taggtaacga
    14521 aattataaaa agagtgtaat tacatcccta tttccggaat atccaacatc aagtcttcat
    14581 tatttggtaa agtgtccagg ctttctattg aaccttcaat gacttttaca cgctccttta
    14641 tttcagcacc ctgcgatata tcttcctcag gctcaattgc aaaatctttt ttttcatctt
    14701 ctgtacctga tggctgtgaa actgctgaat gaaaaatacg actatcaaag tttggcatta
    14761 aaatatattt tataaaaagt cctaacaaaa acaataaaac aaaaagcgcc atatgcatta
    14821 tttttttacc actcaaaaaa cttctacccg gtgagggttt atcgtcaaat atcgatgaga
    14881 tgctgtcctt ttttttcata aataatagag tttaaatata acctaaagag ttcaataatt
    14941 ctcgcaaatc atcaaataag gagtctatgt cttctgtgtt ctctgtcaaa tctctattaa
    15001 agcttgaact acttatgtta tcgtggtttt ctaacaatgc atcaatgttt accataagtt
    15061 cttgcgccct ataactcagg tctgcgagcc tatccttatc ctcttgggag taagtagctt
    15121 gagtatcctc aagaacgtct ataagagaaa cgatcttatc taaaacaacc ttctgtttta
    15181 gtagactttc tttgtatata aaggagttaa gtttatttag cagtacggag taagcaggct
    15241 gtaaattcga tacaactatt tctacatcac cgactgtttc agcctcgttt accaactcca
    15301 ggttttcttt tttaaatgta taaaaatcag ccttaagtat ttttaagtcc gagagctcgg
    15361 cgtcgtccca tatttcggaa gagcccaaaa gacggttaag gcgattttca aaagcctcaa
    15421 atgtatttgc tagtctgttt ttggaaaaaa ttaacgcttg ttgcctatac ctattacaaa
    15481 ctgccgaagt gtcgttctcc tcgttgcatt ttttaaccac ttcaagataa tcagaacgct
    15541 gatctacatc ttgtgcccga acactttgag aaagcagtga tattacgaat aacaatattg
    15601 aaaaaacgag tgcttttttt aaccaaacgt gcatcgtaaa atgtagtata tcttattaga
    15661 tgttagtctt caatgtgttg tttctagtcg tttttctcgt cagtagaact tccttcatca
    15721 gtctcgtcct tagcggtttc ctcagttgct tcaatcttgc tcaacgcatc ttcttcggtc
    15781 tcttcaacta tttcttcttg aactgcgtag cctattttta caactaagtc ttcgtcttcg
    15841 tagtctttta tttcaacctt ggatcgatca taatttagtt cttttactgt tattccttgg
    15901 tcaatctcta caagcccgga aacatcaact gtaaagttat tcggcaagtc tgtcggtaat
    15961 gcttcaacat caatttcatt aagtaataca agtactaagc cttgcccatt tttcactaaa
    16021 ggattttttt cttccccaac aatctcaacc ggtatagtgg ctgaaatttt ctcttttaag
    16081 tttaccttgt gaaaatcaac gtgaagaatt gtagaagtca caggatgaac tgcaattcct
    16141 tttgttaata ctttgtattc agtcccatca accattaaat caataacgtt tgtttcacct
    16201 gccttgttgt taactcttac aaagtcaacg tagttaatag aaacaggcac agattccatg
    16261 ccttttccaa aaataactgc aggaataagg cggttttctc ttaagctttt tgttttttta
    16321 ccgaagactt ctcttttttg tgcattaagt tgcatacaga ctcctttttt agtttcttat
    16381 gtacctgtaa tacttcttcc aaactaacgg caggcacatt aatatacttt cttacttcac
    16441 tgctcattaa atccgactcc aaaggcaaaa caccaagtcc aatagaggtt atggttacca
    16501 tattttcagc acggcaatta ctacaaacag catgggctat cgtaattaat ggcatttcgc
    16561 tgataggcac gaattttgcg ctgtccaagc tggttccgca cttagtgcaa aaggctttag
    16621 atagctggtt ttttaggctt actgatgatg tcataatact atttttccta gaaacaaaga
    16681 gattttaccc ttgtttgcac atttttgcaa ctttaacaca ctcggcactt aaaagctata
    16741 gatagttagt ttattttcaa agaagttgtg aaatcagtgt tcaagtagcc gctataagag
    16801 cccgacagcg accccaaaat aagcctggga tcgtctgaag aaattgaggt accaaaaggc
    16861 ccgtcaaagc taaaagacat taaatcgtcg tttgtacccg gctgtttttg ccaatacagg
    16921 ctgtactcac cgttttcctt tgaaatattc aacatctcgg ggagatcgta ctcaaaaata
    16981 accttcaatt ttttctgagg atcgagctta aagcctacac caaaggagtt atacgctcct
    17041 tccttttcag caaccactga gtcaaaaacg tttgtttcta catcatttgc agaaattttt
    17101 gcgccggtta aaaacgtgcc ctcaggggtt attatgcgta cataatttgt gtagggtcct
    17161 ccgggccaag cagaatctgt gcctgtgtgg tcgtataaaa gctccagcct agcccttaaa
    17221 accccgtccc tagtcaaaga tgttactgaa taagccattt cgttcttaat gtaatagtta
    17281 gctttcgtac ctcctaaatt agcgtttact acctgaagat aatctccgcc ggtagggcgt
    17341 attgctccat cccagccaag atcgttaaaa acggtgccaa gagaactacc cggaagataa
    17401 accattagat gacgctcgtc taaaagcttt tttgtagcta aaaccatctt aggtagcttg
    17461 gtgtcgctca gcccgaacac actttccaac aacttacccc ccattacagt taagaacgtc
    17521 tttttctgct cggatcctgc ttcaaaagtg aattcgctgt gaaattgagt cctttcgtat
    17581 atattttgtg ctgtaatttc ctcgttataa gccgttaaaa agatcgggcc ggtaatctca
    17641 agaaattctt ttgccatatt aagatctaca gcaaaaacac cgtcaaaatt tctcccatct
    17701 accttgaaaa aaagctcttt tataacttca gcgctctggc tgaactcggg ataccaatta
    17761 gcgtttctta taaaaaggct atcttcctgc aaatatgtct ttataggtgc cggggaatca
    17821 atttttatct ctcttaaagc tatctgacca tcggggttat aaacatcgtc aatcactata
    17881 gaagctatct tgccgtcttt aaaatttact accgcgtaag acccgataaa cccgccggta
    17941 ggtcttagtt cgttattgtt ctgaaataat accagatatg attttgttcc atccacccca
    18001 ataatactgg gaaattgctc taaaaacggg cttgctaggt cgcttagata gagcgcgttt
    18061 tgtagctgtt ctttataaga ggcggttcta ttcttcaatt ttggatagtc tatttgatca
    18121 agttcgtaag cagctagctg aaggttttct tttgccactt caagatcttc tgtagcttca
    18181 actatcttag aggtgtctat gggattttct gaagtgggct tcattgtgtc ccacaaatct
    18241 tttagaggca ttcctgctga tgccaaaaaa tatatacttt ttattgaata ctttcctgag
    18301 tttagtgcct gcctagtatc ggtaaaaaga tcttcgcgcc caacaacttt aaaaagataa
    18361 tatgttcgtg acagggccgc atcggcccta tatagctcgt taaaggcttt ttcggaatta
    18421 ctgatagcgt cgtccaaacg catgcttctg gtcgcagaga tagcttcttg ggcaaagccc
    18481 tcggccctca tagcgtgtaa tgcaatctgc aacccgggca taataacaaa aactatcaag
    18541 aaagctaaaa acactaaagc cagagagagt acaaactgta cttttttaga acttaagaac
    18601 cctcctctat cagcgcttga cacgatactt gaagagccgg aatcgcctaa atttgatgag
    18661 gaatataaag tttcaagctg agaaggcttt gtcgacaaac ttttatctct tgcggttttt
    18721 ctaaaactaa aaaatcttct attcggctta ctctttacag aaaattgctt ctccggcgca
    18781 agtatactca agcttgatac aggggcccct ttttcggaac cgtagctatc aaccgtgctg
    18841 ataccttcta tggtgcttgg gttgttgctt atttgatttt gcgaagttat aagtaccgcg
    18901 tttttgtctt caggtgcagg gacatttgtt gacacaggtg tatttggctt ttctccgggt
    18961 ttaattgata cgtcgtctat tcccatagaa gtttcgtcga ccaaagagtc tatttttacc
    19021 tcatcagagg acttagggtt tttctttttt gaaattagac tggccggctt aaatgaatga
    19081 gaactaacat cgtaaccaaa agactccaaa gtctttttta aaccttcttt caagtaaact
    19141 ttttgggacc attttatttt tttaagtgct gcggaatcta aggttatttt atctacccct
    19201 gcatatgaag tagtttttgg tttaaaggtt acctctattt ctgagctggc taggtttttt
    19261 aataaatagg cagtttcaag aacagtacta tattcttcgc taataaaggt gaatatctcg
    19321 cctgatgtat tatcggaaaa cattcctttt accaggccag aaactgcatc tgaaatgtaa
    19381 atatagtttt ctttttctac tccgtcgcca taaatgacaa tatcggtatt ttcaagtgtg
    19441 tttttaatag cttttcctaa actaccactg gcttcaaagc tcattcgtgg tccgtatatc
    19501 tctgagaatc ttgctattct aacatctgat tgaaactctt tgtaatactc ccaggtcaaa
    19561 gcttctgcat atctcttagc ctcgttgagt gtgtatttat tttctacctc tttagaggtg
    19621 ccataataat gatcaaggcc tgctggggat ataagacctt tgtatacatc aactaaagac
    19681 gcgagcatga attttgcgtt gcaggcctta cagaaatcca agattacttt tgtcccaacg
    19741 ctattagtca atagggtgta gtagcttaat gaagaagcat cggtgagata agcttccaat
    19801 cctgccaagt gataaacata gtcaacactt tctatctctt taggcaaacc aacatttata
    19861 tcagcctcga ataaagcgaa tttgggattc tcaagaaatt tattaacgta tatttgttta
    19921 ccggtactga aattgtcaac cacaatcacg cgagcatctt ttaataataa ggcttcggca
    19981 aggtgagatc cgacaaaacc tgctcctcct acaatgagga ctgtcggcga tagtgcagat
    20041 tttttaataa catttgtatt aggcataggg cagtaagcaa cacaacaata taaaatattg
    20101 ttgtgcattt gtataaagta tatgtagagt tagccctttt gacaagcagt aactaaagtg
    20161 ataatatact atcgtcacca aaccaaatga agttgtttgg tgtttttttt tgatgtgcgt
    20221 gtcaagtacg ttgctttcaa caagtacaga cagcttataa agcgagtagc ttattttcaa
    20281 aaaaaacacc atagaagctt gattaatact tgagcccaaa gagctgctta aacaaaagcc
    20341 cagaaaaaag agaaaaatag cggccagaga agctctaaaa gaagtacaaa ggtaaggtgt
    20401 ctggtgcgta attaattaga aattaaatat aaaaaaaatg aacgatcaac aaatattaat
    20461 atcagcagaa ggtttagaga acttgaaaaa agagttgcaa gacttaattg aactcaaaag
    20521 accggaaata gctaaaaaca ttcaaatggc acgagaccaa ggagaccttt ccgaaaacgc
    20581 acagtacgat gctttcaaac acgagcaggg tttggttgaa ggaagaatca aagagcttga
    20641 agatataatt aaaaaagcaa aaataattga ggagagcgaa tccaacggaa cagtaaaaat
    20701 aggctcaact gtgactatac atgtagaagg cgagaaagag gtaattaaga ttgtaggcag
    20761 catggaagcg gacgcaatga ataaaatgat ctcacacgaa tctcctatag gaacagcgct
    20821 gttaggaaaa aaggttggcg atgttgcaga ggtttctgct cctgtgggca aactaaaata
    20881 taaaattcta caaatagatt aatcgagctc gacagctaaa aaccccggct cgcttacaaa
    20941 agacataata cttactacct ataaagtgcg gcatagccac tctttaacat gtaaaaatat
    21001 ttcacagaaa tactattaag ctgattatat tggtttgaag aaaagtatta tcaactgtct
    21061 ccagcttacg caggttattt gctgtaactt ctttgcgctg caaagcgcaa catgttgggt
    21121 aaactatatc tactagttta cgcaagtagt tttatgtaat gaaattataa aaagctcaac
    21181 aataacctat ttttctatct caacttttgc tctggataaa gcatctttag cagatgctcc
    21241 gctaaggacc tcttcgatag aagtgttgag tgcgtcggct tgccttctgt ttccggcacg
    21301 gcctgcaatt tctgaacttt tagcaaacgg agctgaatct aaaaccggcg ttatgtaagg
    21361 atttgctacg gtatctttta agctgacggc gctatacgga ggtccaaaag cacgaatttt
    21421 agatgcttcg ctaaagtaaa gttgttgttg ctccggctgt gccaaaaaat ttataaaatc
    21481 ccacgccacc gctgaatttg ttgaatttga cggcacagca tacatccaaa aacttcccca
    21541 ggaaacaggg ttatctataa gagcctgcgg aacagcggca actccaacat cattaatatt
    21601 cggcatagcc ttcaaaatat ccagaatctg ccatgtaggg acgaagatca tcgaggcttg
    21661 ctctttagta aaggcttctg tagcgttcgg catagcttct gaccagactt tatcaacttt
    21721 tgcaaaattc gtataaaaag taagcgcatc ttgtgcaggc tttgtatcca tttgctcagg
    21781 aacagctata ccggcttgag ccatcattaa ccctactata tcggttgagt ggtcaatgtt
    21841 cttggctgtt ccaattgcgg ctccgcctct ttttagctca ccatcgggac ctctgacagt
    21901 aagttcgatg gcaagtcttc taaactcttc ccaagcggta ggaggaaaat tctgacctat
    21961 ttctttaaaa tgcttcttgt tataaacaag aacgagcccg tcatagtatg cgggaactgc
    22021 atatatttcg ccgtcaaaca cggcactttg agcagcaacc ggataatacg atgttgtata
    22081 ttgagacaca tccattaaat ttttcggcat tggagctagt aaagtaacaa gatcaggcac
    22141 ccatgtgttg tgaaccaatg ctaaatctat tcccgaagac tgctgagaac gggtaaatat
    22201 cctctcctta taatcaactg tagtcataat agacctgtct tcatagttta ttgtcacatt
    22261 cggattttgt tcctgatact tctgaatcag aacgttcatg actccgctgt cttcccaaag
    22321 cccccaaaaa accaaagtaa caggttcggt agtcttgcct ccgccaccaa aaagaccgcc
    22381 aataaccggg agatcctgta ttgtgcaacc tgccaaaaat aacgagatta taggaagtaa
    22441 cattaaagtt ttatacttgt tgggcatatt gaataaattt tacaccatat aaaggtaatt
    22501 aagcaaaggt ttctttgaat taaaacaaat catccgccac cagcttacgc aggtggtttg
    22561 ctgtaatttc tttgcgacgc aaaacccaac atgttgggta aactacatct actagcttac
    22621 gcaagtagtt ttaagtaatg aaattataaa gaaaaaacca cacaggcgca aaactggcct
    22681 aatatcccca atctagaaaa actaaaaagg aaaaagcgct atatctttag aacgtattat
    22741 tcttgatgtc gagctaagct tttttcagat ctatacaggg atagagagag aaacgtccaa
    22801 aacagtaata gaatttgatg cgatgtaaaa ataaagtgat caaacaaacc aagcaaaaaa
    22861 tataacaata caaagcataa agaaccataa tttttattta tgtaaaaaaa ccttagtgtg
    22921 taaagaaaaa aagataagaa cagaactaac ccaattaaac cggtttctga acctaaaagc
    22981 aaaaatatat tatgcaccgg ctgaggaaat ctgcgctctc tttcaaaata gtaagattcg
    23041 agtagttgag gggatgttgc caagcctgta ccaaacaagc tgttatcaat aataatatgt
    23101 tttgatttgc ttgccagagc agctcttaca acaactgaag gagagtatac agacgtaaaa
    23161 tccggtacag tattaattaa aaaagaaaaa gaaataacaa tacttaaaat aaatatcgct
    23221 aacgtaactc ctgcctgcct attcttatta ttagccgaac tcgaaaaacc atagtacagt
    23281 aaaaagccta ttaaaatagc taaaaaagca ttgaagctgg aagcacttac cagtacaatc
    23341 acgcacattg taaaacaaag tactaaaagc tttctactta caagattacg taaatctttt
    23401 aagttcgtga ataaaaaaaa tacgagaagg ccaacatacc ctgcaaaaac attcggatgc
    23461 ctaaaggttg agtagggagg aacatatttg ttaccgaaaa aggtttctac cgaaataccg
    23521 aatgttgcac tagtgtaggg ctgctcacct aaaactaggt agttgttgaa cacagatctt
    23581 tgcaaaacta attgcacagt tccaagaacc aagaataaaa aaagggagaa aaaagcaact
    23641 tttaaaaaaa ccggataatt gctgtaaaaa gtctgagcgc caagctctat atcctctaag
    23701 tttccgccgg tagaaagagg tagattttca cttttatcac tttctagcag cacgattaca
    23761 gcactaaaac aaaaaaatgt atataaagaa aatcttaaaa aggccactaa agacgccgta
    23821 atattaacgg atcctatgga agatagaccg aaagagaaaa taaaaattgt tgctaacgga
    23881 atcagccatg atttatttat tttgctaaac gaagatttgt agtttgccct gtttttactt
    23941 ttagtagcct cgaaaataaa aactaatgaa aagcaaaata ccagaatatc ctgcacgtaa
    24001 agggtaggaa ttaaataatc tacgagaaga ccgttaacat agctggacgg gataataaaa
    24061 tgctttccga gatttaacgg aatagcaaaa actataacta ccaacaattg aaccataaaa
    24121 ttcagtttta tattcttaat ctgcatatta tttttcttac tctgcgtgac ccctaattta
    24181 gatctaaagc aatattatgg gcatttaata taaggccgtc atctaatttc gccaaacctg
    24241 aagccggcag ctccaagctt ttggcaatca aaaaatccgc taaatgtgga tttttcgata
    24301 gcacagggcc gtgtaaatac gttcctatag aattcatgaa atgaattccc tcagtgccat
    24361 ctttcccatt atttccaaat cctcgtctta taacagcaaa ctcagaagac ttcgtgccaa
    24421 gattcgttct tcctccatga ttttcgaaac ccactaagct accgccgaag cctgactcat
    24481 gaggaaaaga ggggtcgcct gttattgttg aggacatagt gcagactaca ttacctacac
    24541 accgcgcggc gctattaccg ggatgttctg tactaagatc aaaaataccc aatccctgca
    24601 aagtagagcc atcagcagat ttataataat gtcctaaaag ttgataagaa ccacaaacaa
    24661 ataaagaaac gccaccggaa ataatatagt ctcttaaaaa cgaggctttc gaagtagtta
    24721 gatctttata catctccttc tggctagaat ccggacctcc ccccataaaa acaagatcaa
    24781 tgccggacat aaaaaattta ttaagcttag tttccaagcc tacttcgtaa acatctacat
    24841 cgatgttacg ttttttacac ctcgagacta atatctccac attaccgtta tctccgtata
    24901 ggttcaataa atctttataa aaataaccta tcctcagttt cataaaatct ttctcccaag
    24961 caaaagcttt ctaaaatcaa gcattgctga atagttagga agaaccagaa catgctcggc
    25021 ttttgattta ggaagaacct gcctaacaac ttcccctatc aaagggttaa ctttagacat
    25081 atcaatacta cagcccgcat actgcaaacg aacagccatg tcatagcatc tcgtaccgct
    25141 aatatacagg ccgttaaaca ttgcgagctt gtctttaata gctgtaggat caatatcata
    25201 aatccacgac acgtctcttc cgtcaggaat attgtcattt aagataatca atacatctgt
    25261 gttatcagga gaaaagtgct caagtaaatc aagattatgg gtaaagcttg ccggattttt
    25321 tgctaaatac aaatggtact ttttattaaa aaaatctatc tgctcacctc ttccgtaagc
    25381 actctcaaaa gaatccaaag agctcaatat accggttaaa gggatgccta agtcctgtac
    25441 tagcttggtc gcagcattta tatttttcgc attaaagctt cctttaaggc tagtcaggct
    25501 gagcctttcc gaactgtcgt caaaaaaatg catgtctgca gacaatgtct taaacctagg
    25561 ctcggcagaa tcagcgtaaa tgcgaacaga aggaggcagc tttcgaatag actgttgcca
    25621 gcgatctaag actatatccg tctcaccata tctatccagc tgatctctag ataagttgag
    25681 gagtgcgatg gcctttggat ctatgtactc taaaaaccta ggcaaagtaa attcatcaat
    25741 ctctaataca caataatcta cagaaccaat atcggccaat aaaattgttg tgaccaaacc
    25801 gttaagcaga ttagcaccgg tcttgttatt caaaacttta tatcccgatt gttccaatat
    25861 atgagttatc aatttggctg tagttgtttt tccgttagtt ccgctaatta aaatgtaacc
    25921 ttttcgacat tgaagttgtt taaaacttaa aattgagggg aagagtttca acaccacgtg
    25981 ccctggccaa acataccctg agcctatacc taacctagta cttaaattga aaattattaa
    26041 cacaatgaat tttgataatg caaatctcat ttctttttat tgttttttct tatgtatcaa
    26101 cagtcttatc tttttttagt atatataaat taagttgcag tagtattagt aagtgttgat
    26161 aagtgtatat ctctgcaacc tccctgtttg cagtaacaga tttacttctg tattgtttat
    26221 aactcttttt cggcttgttg tcagcatttt cttatggccg gcgggcgttt ttctttgtcg
    26281 taaagacgca aatctttgtg ggtatttctt tatttcttgt ggatattttt gtgtaaaagt
    26341 gcgctgattc tcacggcttg gcttgtgatt gttgttttct ttccactaat cctcgtgttt
    26401 tattcacgtc ttttctacat tttattcaca attaaatgcc gcctgttttt tgcttttgct
    26461 gtgagctttg tggttatttg tacaactttc tttggtacgt tgtggataag ctacggttta
    26521 ttctccagat gctttttgct aacggtgttg tttcgcctct ctttgctttg aggcacttta
    26581 ttttattggt ataaggcttt ttttattgtt actacttctt gtctcattct ggtagattcc
    26641 ttaatatcgc tttcgatctt ttttaccccg tgcattatgg ttgtatgatc tcttccgccc
    26701 aggtattctc ctatagtcat gaaaggcgtt cctgtaagtt cgtacattaa gtacattgct
    26761 atttgccgcg gtagggcaat ttcctttgtt cgccttttac cttttaagtc gttgtgtttt
    26821 attgcgtagt atccgcaaac cgcttttgct atttggttta ggtttattgg ttttgaaggc
    26881 tcagttttta tattgttacc taaagcttgt ttagctgttt caattgagta atcggcgccc
    26941 gttgcttttg aaaaaataat aacctggtta tacgccccct caagctctct tatgttcgag
    27001 gtaacatttt ccgcaataaa atctattatt tcgtttggca atttatctcc gcttctgtct
    27061 ctcttatttc ttaaaattgc tgtacgcatt tcataatcgg gagcctggat atctgccatt
    27121 atcccgctct taaatcttga tgtaattctc tcttcaatat ttttatattc ttcgggaggt
    27181 ctatctgagg ctaagactat ttgtttttgt ttcatctgta ggtcattgaa ggtatggaag
    27241 aactcttctt gagtagaatc ttttccggca ataaattgga tgtcatcaat aattaacaag
    27301 tccgcgttgc gatacttttt tctaaatgtg tctgtgctgt agccgccttt tcctcctttt
    27361 cctttcataa tgttttcaat tagttcgttt gtaaattgct ctccggtgca atatataatt
    27421 tttgcctccg gattattttg caaagccctg ttagctatag cttgtattaa gtgtgttttt
    27481 ccaagaccta ctccggagta tagaaaaaaa ggattatgtt ttgttccggg gttgtcggct
    27541 attgtagtgg ctatggcgta tgcaagacga ttagtatttc ccattacgta gttctcgaat
    27601 gtaaataaag gatttagtcc gttttctttt gcttttttaa ataaattaag gcttgatact
    27661 ttatcatcgc ttttgtttgt ggtggcgaac aaaggtgaag tgttaatctg atctcgtgtt
    27721 tcttttgttt gtccgatttt aacaactaaa tcaagatctt ttttgcaaat tcttttaatg
    27781 ctgtccaaaa taaaagcttt ataaagagtt tcaattttat ttttagcaag ttttgattcg
    27841 caaataatct ctactttttc ttcatccaaa ctaacagcct cggttctaga aagccaggtg
    27901 ttatagtgca ttcctgaaaa atttagttcc atttcagcta gcaccgcttt ccatagtgta
    27961 atttcggtta gtgtttcttg cgacattgtg aataaatatt atacgagtta tccacagccg
    28021 taaagcaacg caggttggag tatttatgca cgtttgcttt ttactattag tgttagaatt
    28081 ttcttttttg aaggccgggc agtttcatct atttgactta tttctaactt tatcctacaa
    28141 ttccccatag ctatgcctaa aagaacttgg caaccaaaaa aacgaaaaag agtgaaaaaa
    28201 cacggattcc taaagagaat gtcctcaaag ggcgggcgac gcgtactgaa aagaagaaga
    28261 ctaaaaggca gaaagctttt ggccagagtt ttaaagatta aataattacc aaactccaat
    28321 ctccactctt ccactgaagt gatcggccga aatattaata tgttacccaa acaaaataga
    28381 ttgtctactc gccaccaatt taaaaaagtt cgatacttat ctaaaaaata caatcaagaa
    28441 atagtatccc cttactttta cttgtcctac tcttatactg accctgaagc cccgacagag
    28501 gtaggttttg taattggtac aaaactgcta aaatcagctg ttgccagaaa caggctgaaa
    28561 agagttttta gaggctgtgt tcgagaaaga ttgtctgaca ttaaaaacgg ttactggata
    28621 gttataattc caagaaaggc aagcattgaa gcgccctatg aaaaaattag cgctgacatt
    28681 aataaggcta tacaaaacat ttgtatctcc gggcagacta ggaattaaag tttgcagatt
    28741 tgagccaagc tgttcaaggt atacatatga agctatacaa aaatatggta ttttaaaagg
    28801 tgctttattg ggtttgtata gatttttgag atgtaaccct tttaataaag gcgggtacga
    28861 cccggtgaaa taaccaaaat ggaactaata aaagaatttt ttaatttaat acttacagta
    28921 ccgttatata acttaacaat gttcctgtat tttttaacgg gaaatttggg actttctatc
    28981 attttactta ctgttttgat taaagcgatt ttggttcctg ccaccaatcc ccagcttcgt
    29041 actatgaaaa agcaacaggc acttaagcct gaacttgata aaatcaaaaa aaagtataag
    29101 gacgataaga aaaaacaagc cgaagcccaa atgactcttt ttaaagagca tggtttaaat
    29161 cctggttcag gctgtataac gatgtttgca actattgtgg ttatgctcgc tctttacaca
    29221 gttattagaa aaatcgcact agccactgat atagatcaaa taaatcaaac cctgtacttt
    29281 gaggctttaa aggtcacaga gggtataaaa acaagctttt tatggtttga tttgtcgaag
    29341 cccgatcctt tttatgtttt ggctgttgta tcaggtattg tacagtttat tgtttctaaa
    29401 atgacaatga gctttaataa gtcgggcgag gctgcggcta aagaaactcc tgacagaaaa
    29461 gatgatatag catataatat tcagcagcag atgctctata ccatgcctat ttttaatgtg
    29521 gtaataggca taagcttgcc tgcaggtgtt gtcctgtatc ttgtagtatc aaccgtattt
    29581 tctgcctttc aaacatattt tgtattaaga gaggatatta gtacaatgcc cgtacttaag
    29641 aaggagaaaa aatgaataaa gatcaattta taactcaata catgaaagat gttttaaacc
    29701 atctaaatgt tgcaactgta gtaaatattg atgttgcaga agattctgta aaagttgttt
    29761 taacaggaga tgatctaaac tttttgattg gctttagagg aacttcttta gatgctctac
    29821 aggccttgtt ggctttatct ttatataaca aatacgaaga gtggtttaat gtgtttgttg
    29881 atattaacgg ctacaaagaa aaaagattcg agcggcttga ggatttggca aagaaggcta
    29941 tagataaggt tcgcttcttt tcaaaagaag tcgccttacc tcctatgaat tcttttgaga
    30001 gaaaatatat ccacgagttc gtgtcttcct atgatgacat gcagacagaa agcaccggag
    30061 aaggttccga aagacacatt gtagttagca gttcgtagtt ttaggccact gggctagata
    30121 atgacagtaa aacttattta aactcaggat aaagtagagt ttgctacctg tagtatatta
    30181 aggtaaccta atacagtatg aaatttacat gtttaagaga aaatttagca aaaagtttga
    30241 gcgtagtttc aaaagcaatt cctatcaaaa gcacgtttcc tatattatcg aacgttttaa
    30301 tctcaactca taacggtagg attaaacttt ctgcaacaaa cacctcaacc tcaattacaa
    30361 catatgtagg agcttccatt gaggaagaag gcacaataac tattccggca aagatgttag
    30421 ctgaatttat cagcaactta tccagcgaaa ccgtatcagc agttttagaa aataatgttc
    30481 ttcaagttaa tgccgggaga acaaagtcga aatttaacgg tgttccgtcc gacgatttcc
    30541 ctcccttacc tgagctgaaa aacgagtatc tttctatgga gttggatcag aaagttttta
    30601 gcaacgcagt aatgcaggta ggattttctg ttgcacttga cgactctcgc cccgtattca
    30661 caggaataat gatgagttac tcagacggca agttaacttt agtttcgacc gacggataca
    30721 ggttaagcga aagaattgtt tccctcgaac cggttttagc aaaagagtca cgagagttca
    30781 gtgttgttat acctgcgaaa actcttgttg aggttgcgag aacattcgct tcctcagaag
    30841 agaatataaa ggtatacgta gatgaggacg aaagtttatg tattttcgaa tccggagata
    30901 ctgtcatatc aacaagactc ctcgaaagct cttttcctga ctataaggcg gccattcctc
    30961 aagccggagt agtaaccgca aacttcgctt cgtccgattt acttgaggcg gttaagctca
    31021 caagcgtttt tgtaaaaggc ggttcgtccg gtgcgctaaa aattgcagtt gatccggaaa
    31081 cctcgttgtt aagtgtgtct tctgcggagg ctgagtttgg gcacaatgac actagtattc
    31141 cggctgagat tcaaggagag tcaatagaac tattttttaa cttcaagtat ttattagact
    31201 ttttaaataa tattaaatac gaaaaactta cacttgttgc tactggccct gactctgcct
    31261 gtgttttgag gtctgacgaa gactctgaat tcatttatgt aatggttcct ttgcagctaa
    31321 acagctaagt ctcaacgttt taaacaaaac tttaatatta ataacacatg caggcattta
    31381 tggttgatgt gcagttattt aaaactgtgc gggtggagca ctggggtctg gcagattctc
    31441 gtctgccggc tcattgttta tatcagtttc gatattttct aggtttaatc cttcgcccgc
    31501 ttcactttct tgaatggggt taagtgtgtt ttcatattcg gtggccgcat ctttactaac
    31561 aaagctactt tgatcgctcg atgctttaag ttctacagat cggttcatct gaggcatttg
    31621 agtctttgta gttattcccg cttcagcctc agcctctctt ctcattctca gaatttcttc
    31681 agggttggta gttattaaag cgttttctgc tgtcgaagcc tttattttta tagcaacgtg
    31741 atttgcgcct gcaaaaaata aaccctcgcc cttgtcggcg gataaaagta gtcttttttc
    31801 tccttcagaa aggtagaaag tttctgctac tttatctatg gctgccgggt gttgttttaa
    31861 taatatttga atagatgagt ttgtaacgat ggctcttcca tactctgaat ttaaaaagtc
    31921 ctcaacgtct tgcgatatgg ttgtcagtcc aaggtagtat tttcttgcac gtttggccac
    31981 tccataaatg aaccgcgcgc tgtcctcatt ttgaagtaat gaccacgctt cttccacaat
    32041 aagtattctt ttctttaagt ccttttttat ccttgtccat acaaagtcta aaattatgta
    32101 gtaggcaata ggtcttagca cgtcttcaag atttctggtt gagaaaaccg taagcttgct
    32161 gtttaaattt acagtcgatt gagagtcaaa aatacctaca agtgagcctt taatatattt
    32221 ttctagtctg tctgccattc ctcgggcttc gggctctgca gcacccatca ggaccttata
    32281 aagatcttcc ataataggag gttctttatc tctttgtgtt gcggggtcag tcgttatgcc
    32341 tttaattcga tacgtttcca taagggccct gtccaaaaga gcctcctcta tgggcgacac
    32401 tgtgcctagc attagtttaa ataaagtgtg caaagaaagt attttttgac ccaattcgtt
    32461 ttctcctcct gtagaaactc ctgccaagtc aaaagggttt atcttagaag gcgagcttga
    32521 agagaagcta atgtagtttc cccctacagc gtcagaaagc attctgtatt cttcttcggg
    32581 gtctatgatc ataacttcgg ccccaaatat cattaatctt aaagcttcaa gttttacaaa
    32641 ataagattta cctgcgcctg attttgcaaa aacaacgctg ttagcatttt ctagcgaaaa
    32701 cctatcaaag attaccaagg acccgttgtg tctgtttatt ccatacatta tgccttcgtc
    32761 ggatgttaga tctgaagatg tgaaaggaaa ggttgtggct aagcttgtag tatccatgtt
    32821 tctggttatc atcagtttgt ctaaggcaat aggcacagtt gactgaaaag cctcttccat
    32881 ttgtagagag gctgttttgg aaattagtag caatgagcct aaagtggcct caagtctttt
    32941 tgtaatgtta ttcagttcgt ctaggctgtc tgccggtatg gttatataaa aagaaaattg
    33001 aaaaaatttt tctgcccctt ttactagttg ttcttgtagt cctttagcgt cttgtagagc
    33061 tatttcgact cctggatcgt taagcctgcc tttttccttg ttagtcatta cggtggcttc
    33121 catttcagtt attttccttc gaagatcgtc taaaacccct ttagattcaa cagggtagta
    33181 gaacatagag atatccaagg agtgttcaaa gttaattacc ggagacatcc agttggctcc
    33241 tacaaaccta ggatatcctg atacgaaaag agttctatag tatttgttac cgatttttat
    33301 gctgctgaaa tcaacctcta acgcaggcgg ggcaattata tcttttacat ttatcatacc
    33361 gtctgctagc cgtgttgcgg ggttctcttg gtcttgtact tgttgcacat cttccgcctg
    33421 agctttattt tgttttttat tctttttttt gaataacatt aattttggca tattattctt
    33481 ctataatagc aggattaacg atcgctgttt tataatcttc tatgtttgtt cttattcttt
    33541 gtccgtgtat tgtgctcgga ttgtaaatat caaaataaag ctctaccagc tcttgggttg
    33601 tcattaccgt agcctttact cctattctgt taaattctga aatcacatgg gcaacttttg
    33661 gggtcagttc tgtttttgct ttggttagag ctttatctac attcacaggg gctctttttg
    33721 aatgaaatcc gaaaagcaca aaaaaccact caaaaggatt agttcctttt ttaagatcaa
    33781 ctattccgcc ggtaggaata acaacataaa attttttatc taaaacatcg tttttttcga
    33841 taacttcttg tacaaactgc ctatactgta ttgcttgaga tttaagtaga ggatcggaga
    33901 ctttttcctc gtttctctcc aaactttcga gatactttgt aatatccagc ctttttgatc
    33961 ttattactat ttgaatcggg aaggagaagg agtttaatag catggagaaa gcagagatca
    34021 tcgcatcttg ctcgatttcc gatagaaggt cgaagttaac agcggttgtt tgtaacacag
    34081 agcatacact tccgtttttt aaaaccacga aatcgtcttt tattgtctct atatctaaat
    34141 gttcttgagt tgtcgcttta atgtctgaca tatgatgtac ttttttttat ttcgcattta
    34201 tgcgaaactc tacctttcaa tttttatctg ccctttatca gaataggtgg tattatcttt
    34261 cctgcgacct ctactttaat tatatcaaag ctgaggttag ttcgtttaga cttgtcagtt
    34321 tcgatagtat aagtaccgtt tagcaaaggc gtggttattg caaactctcc aagagggttt
    34381 gtttttagtg ctcttacggg ttcttgggcg tggtttttaa ttatcaacac tatattctct
    34441 aatgctcggt tattttggtc aaccactttg ccactaatta tgttaggtat agtgttggaa
    34501 atcccgcttt caataaattc aggggtttcg ccgtgaatca ctttctcggt tctttctctg
    34561 tcttcctcaa tagctttttt aacaaaagac ttttcagtta gctctgtgta tttttcctta
    34621 atctgtgagt aatttttttc tgttgcttct ttttcttttt ccagcttttg tatagttgta
    34681 attttttctg ctttatcgtt gtctcctttt gagtttgcaa tgtcctgttt tagcctttct
    34741 atttgtatgc tcagctcctt attttctctc tgtagtttag ctagaagatt ttttatatct
    34801 tgccctattt ggattacggg ttgagtattg tcgacgggca ttccaaaagg actttgtgtt
    34861 gttgtgatta tttcgtcatc cggaccgaat tgtgcgctgt accctgtatt cgtgctggtt
    34921 gtattgatag tgcttgcgtt gtttgcttcg tgattactcg ggaaagagct tttttcttcg
    34981 agcgcggaat ggttcatttg taagctgcct tggacatttg atgttagggg tatctctttt
    35041 tctacaggtt tcgctgctac aatattttta tattttgcat ctcgctttat ttgttctaaa
    35101 agagcgttaa gctgctttgt tttattttgc agttcttcat caatttccat ttcttctgag
    35161 gtccttaggg ttttttcgcc ttttattggt aacactatct cgcctttgct gggtactagg
    35221 tttgtaaagg ctctgcccga atgtctgtcg ggcgtgattg gcgcgggttc aaaatacgag
    35281 gtggtgagaa actgtggctt ttcagatggg ttgtatgcgt tgtcaaactg agcttcagga
    35341 ataagatgtt ttggttctgc cgttgctgct tgtgttgttt gtgaggcgcc ggcgcctgcc
    35401 ggcgcctgac ccccacttcc ctcaatagtt ttcggtgctg atccaaagct taaagacccc
    35461 gcgcctgggg ccccggctat gtttccggga acagccgcac tgtattggtt actgtccagt
    35521 cttttttctt cggacggact gtgagatttt tgaaaaccag cgtcatctat tactttagcg
    35581 tgagctgctg gtgctatagg tgcagtagcc gcagtttgta tgcccggccc cattcttttt
    35641 tgaacatttc ctcctggagt ataggggttg gcctttgaaa caggagttcc ggcaaaagct
    35701 gctgctacct tgtttatata cacatgttct tgaaaatcca acggatcgtt gtttggggcc
    35761 gagtcttggg cggtaagaaa ttcctccagt tttctcctgc ttgtagtagg ggaaagcgtt
    35821 attagctctt gtcttacaac gttcaaattt tcaaaagtaa acgctgccgg aatctcgggt
    35881 gtttttttcc agattctctg gtttggtaaa tatatagact taataaaatt tacaacccac
    35941 tgatccagcc ctctatcttc aataggtaaa aaagccaaac ttagcccaaa cagtactaaa
    36001 aaaagtataa taggccattt aaatatagag tttacaaaag cgaatacgcc gtaggcaatt
    36061 ccgcctgaca acgcaaggta catgaattgc ctcatcgtta ggtcaccaat aatcttaaat
    36121 tccacatcca ttatgttttg tggaagcgcg tgttgcttgt ttgttgtttg cgtgtcgttt
    36181 aaaggcatag tttatgtgta tttattgtat tgtaggtgta gcggaaaaac aagttacaca
    36241 ggttgcgcgg ttggtgtttt tacaacaacc ttttttgttt gcaaattctg caaacattag
    36301 aatttagaaa aagtgttttg gtgcctttga aaattggtat attaatctac ctcttcttat
    36361 cgtctcctgc aaccaatgcc tttactttat ccggagcttt gcgtgcgtaa ttatagccta
    36421 ctaggtatat aattagggtt agggcaccct tagtaaaata tggcatagga ataaggcccg
    36481 tgctggatgt tcccataata gttattgatg ctaccctagt tattgctatg gttgcttgcc
    36541 cggcaagtat tcccagtgca tatcctgcca ttgttttaaa ccatccaaca gtcgcttttt
    36601 catttccagg aatcgcgcca aaaacaaaaa taagaggggc ttttataacc tctatcaaca
    36661 tttttatata gaaaatgaac agctttactt gcacaataag gtataagatt acaatcatga
    36721 ttaatatcgt gaagagaaca atgaatgcta cccctgcggc tgggataaat gcctgaaata
    36781 taagcattgc aaggataaag atctcgaggc caaatcctat gttggcagca gccacaactc
    36841 catttgccgt taaggtattt gcgcttgttg tttgcgctat gtctctaata accccaacaa
    36901 ccgagtattt ggcaacatag gccaggtttg caattgctgc acctattggg tagctaaagt
    36961 atattagaac aattgcaagt attaatcttg gtagtgcata ttgaaccgta accgcaactt
    37021 gtgcgctaac ttttttccta tttattatca taaccccaac tactattgaa acgatggcga
    37081 gtaccccgaa tgcaacgttt cgtgacattg cccaaaactc gtacactata tctagccaag
    37141 gcgatgtgta ttgaagtccc gtttgttgag caaataccgg actgatcaaa ggtatgccct
    37201 ccgtttctct ttgtatgaag tatgccatgt taactggcat gggttcagtg ttcgcctctt
    37261 gaaaggcatt tgctaatcca aggaggcttc ctttgcctct agcatcaaaa tctcccccat
    37321 tgcttgtggt tggttcagag aagttgtttc ccaaaccttc gcagtatgta gggtcttggg
    37381 gttgacagtc ctcagcatcc atttgaagat caaccccttg agctgagtat tcgcttagag
    37441 cgtatccgcc gcactgtgtg acctgggtag cgtttccgtt aaaagctatg cttgaggctc
    37501 ctgttaagcc tgcagcatac atgctcccta ccgtgggcat tctaatatct attgcagagc
    37561 ttttacctac gttttcaaat gtgtcaagtt cggggtcaca gttagccgca tatattgacc
    37621 ttgatgcgac tgttccgaaa agaacagtta aagtaagtaa aaaagagaga agtttaaaat
    37681 tttttgtgtt tgtaaactga gccatagtgt tagtaaaata atacaagttt attttaagaa
    37741 aagcaatttc attatagaat aaactaatgg caagttcgaa cccattttat gttggttatc
    37801 atcctgacga caaaccgact caaataaaac ccaagaacat actgcagggc gcagatcccc
    37861 aaaaagcact tgatagtctg ttaaataccg gtttgctgga ccaagctctg caagacatag
    37921 acgtgccgag atatgttaag gaaaaaataa agaatgattt cctctcctct ttagcggtgc
    37981 agataaaaga cagctctgtt tcagcggccg atcttgcttt actgccccct gaagcccttc
    38041 aagacatgca agggtctttt ggtataaaaa taagcctcaa cccgctagat tggaaagatg
    38101 accctgtcaa gcagttgaag aaaacactta gcagttgggt aaaggctgcc tatgacccgg
    38161 acgatttccg tactactaca atgatgaagg tcgctcagtc tgtttacggg aactcaaagc
    38221 cctatactag aacaagttct ggggcttcga gaaggtacat aataagtgat atctccgata
    38281 gactagctga tttgcagcat aaaatagatg agtctgcaat ttcatcggcc gctcttagag
    38341 attctgcaag agccgggaga aatagagaaa tgcaagcggc ggtagcggca ggagacccta
    38401 acccgttaaa aatagttggc tatgtaacga aaacggaacg gaagtcaatt actgtaaaag
    38461 acgcctcagg cgcagatgta accataagtg ctgataaagt ctcggatcct tactcggacg
    38521 ttgttcaaag ttatatctct tttttacaaa gctcttctag cggaacttcg aaaagagaag
    38581 gcgggatgga tggtttgatc tcatcgggtg ttggtgcaat attatccgaa attgatgtcg
    38641 atattgaagg tttaagctta gccccaggag ttacagacta ttcggataaa gtaagggctc
    38701 tgatagatac aagatttgcg ggacagcccg ccgctgtgcg cgatacttat gtttcgcaaa
    38761 tattatctct tcactctcag caagagctgg caaagtcgat gtacggcttt aatgagtttt
    38821 tatctaatgc caaccccgct aagagtttgg gtatttctgc cgcgcttgac cagtatatgg
    38881 cagtgggaag atcgcagcgg caggctttgg cagcagaccc tgcttttatg gctcgtttta
    38941 caaattattc tgccgaggct gcaaaggcca gaacagctct ggaaaatttt aagttaagcc
    39001 ctgaatatct tcgcgatccg gataaataca aaggtatagt taaatccttc gagagaaact
    39061 ttaaacagct tgactcacgg gtggcgcctt tagtcacggc tttccaagct aacccttctg
    39121 ttaactcggc ccgcgcactc gcgactgcgt ctaaaagaat cactgcagta ggtctaactg
    39181 cccctattgc cggaggagat catcttcagc gcgccccttt aagaaaactt tacaggcaga
    39241 tgtatgaaga tcacctaagg ccgcaaggtc cgggtattat aaattccggc ttacttcaaa
    39301 gaagccttgg attaggagtt ttatttaatg aggcggaaaa aatacgaaac gagaactggg
    39361 cggaggacgt gctccagctt cttgagggag gaaagctaac cgatactttt gtttggatgc
    39421 gtgtaaaaaa tacttttcct tatgttttcc caagttattg ggtaggaaga gttttagaca
    39481 aagtccagta tttcgggctt gttattgacg agagctcgat agaaaagact acgacaaccc
    39541 tttttggaat taggagattt tacaaagatt tgcttgagaa ctcggacgga gggggcgttc
    39601 ttggattttt atttgtaaac aaagtaaaag ttgaagccac aggtttcttt ggagatatag
    39661 taaaacttaa tttgaccgga ggtgcccaca tgaacggcgc cctagccatg tctaaatggc
    39721 taatcagcaa tgcggggtcg gcaaacattt ctgacattga cgacatggtt aaaggcatgc
    39781 ttaccaactt tagatttcat tatcttgccg acggtagcat taacatagcc aacacaaagt
    39841 ccgcacttac ggacttttta ttaagggacc caaaatataa gaatcttttg cctactttat
    39901 ttaaagacgg tgctgatctt gacgattttt tgggaaaatt tattgttttc aaacaatatg
    39961 tagatgcaaa gctcgctaag tacctcggga tatcgataga catgctagat caaggtctag
    40021 gtctcgagtt cgttgttttt caagagctgc ataagtttaa cttaagcaat gataccagag
    40081 gcaagctcgc tgttaagtta atggggccgc ttcagaaaat agccttaaaa cttaacattt
    40141 tacaaacaaa catattaaac aaatttccaa ttaccagact tatttttggt ggccagaacg
    40201 ctttactagg attattgttg ggtggaactt atgcgcttaa atctaatgta gccgccttca
    40261 tcaagagcgg tcttgcaaag ctttttggaa agtttggcgg cggggcagtg tcgggttttt
    40321 ttgcctggct cgggccttta ggggccatgc tagggaaatt tgcggataag ttactaacga
    40381 ttttaattag aaaaggattg gatatgttag aaggcctcac taaggccatt attcacttgg
    40441 acatatctta ctttacaaaa tcttatagca agtttgttga taacacaact aaaataatag
    40501 tttacctttt agctataccc cttttgctga tgtttttatt tgcagccatg attaatactg
    40561 tatttaattc ctcgactcct ccaacaagtc aggccgcggt cggacagagc actcctttaa
    40621 caggaggctg ttttggttct ggctgcggtt caggaaccgg gagtacctat gagtgtggta
    40681 tttctaaaac cactggtaac acgtgtattt ttaaagatgg caattacagt gttatgaatg
    40741 gttctttaaa cataggcgct gattctgggc acggctcaaa tagttattgg gactggcttg
    40801 ttgcgaaaaa agtaatagga tcgggccaaa agtgttcttt tacactaccg atgctcatta
    40861 ctgacggaat tgggcccacg cgaatcacag atagcggctc aatccctggc agtttttatt
    40921 gcagaggtga cggcactacc acagaatccc cattgtatgg gtatgctgtg gactattata
    40981 cagccccgaa ttgcgggtcg gtctttgcgc ctcccattaa cggcaactct gtgacttgga
    41041 acctaacccc gtaccccaca cttacaggcg cgggagatgc cgtcttttta actactaccc
    41101 aggggggaac tacatacaga ttaacccttc ttcatgtagt gaatatagtg tctgcaacat
    41161 ctgttgatac aggagatttt attgcagata tgtataattg gcaggtttca ggaagtgaca
    41221 actctcatat acatgttgag tatgcagagg tgactggagg ggcagtaacg gttatggcat
    41281 tagacgatcc ttcaaccggg tctccggcgt tttgcaggta agttaattta acaaaagaaa
    41341 ggttgctatg atagaaaaaa taaaggcttt ttactataga cacaaaataa cgatctgggt
    41401 cgcaggaggg gttgctacga ttgtttttct catcattgta gataatctag tagcaaatct
    41461 ttcaacacaa agacctagtg ttgagcctgt ctacaataca aaacagcaag atgtaaaaat
    41521 attaaaaata tttcctcctg atggggccaa ggtttcttcg ccgagcaatt ttgaagtcat
    41581 ctcagtcact ctttctgcgc caataaaaaa aggatccttg gttataaaaa ttaatcctta
    41641 cataggatat acaatttcct atagcgacga aaatccatct atcttgacca ttgtacccaa
    41701 aggccagggc tgggctccca acgtgaccta tactattgac ttcttgcacg cgttggacga
    41761 ctccggaaag aaggtttatt taaactatcc ttatgtgttt ttaaacacac ctcctgatcc
    41821 cgcaagcata gtctttcctt tttaatctaa aaattcctga cgcaatgtgc gtcgtgtcgt
    41881 cgtgaggtta tagaataccg caacctttct ttaaagaaac taaagtttaa ggttgtgtat
    41941 gattctattt agtgcggttg aaaagatccc aggctattac tgacagcccg ggagccctct
    42001 tatgataaga ttgccgtttg tatcggtaaa tattccgcat attccggcaa ttacatcgtc
    42061 aaaaaaattc atgcttgctt gcaggcctgt aacattcatt ataaggcgta gtacaacaaa
    42121 cgatcctatt atcattaaaa accctattac agcctgagtt cccgagagat ttgcccccat
    42181 tatgcctttc ggatctcctt gagcagtaat gtacttataa gctgtaataa taaccatcat
    42241 tgaaaaaact gccccggatg cgtaaagaag tatataaacg actctaatta ttgggcacat
    42301 aagttgagtg aactccggcg catcaacaga ggttagttgg tcgcaaccaa gcccggcaac
    42361 ccctgttact gcgtatgttc tttgaggagc aatcagcagc attgttaata gagaaccaaa
    42421 aatgactatt tttttaaaaa gttggcgttg cattttgtag atctcctcct attccaacaa
    42481 tatttagcag tattattctt ataagtaaaa cggcaaaggt tagcaaaaga cctagcaaag
    42541 aataataaaa agatttcatt gcggtcgcgt atttttttgg gtccgcaaga ctagtgataa
    42601 cccttatgcc ggaaaatcca atgaaaatta ttgatagaga tattcctacc ccgatcataa
    42661 cattcagtat aagtcccaga acgtcaccta tcgagttgaa attggatccg gttagtcccg
    42721 aggcactgcc gtttattttt tgtaaataat aaaacatatt tttcacacac caacacggcc
    42781 ctgcctttct tggcagggct gtgccagttt aacccctagc tcaaacatta gaatggggtg
    42841 attccgctta taccgctgtt tgtaccactt attcctacaa cattctgtgc gattatcttt
    42901 attgtgatcg cgccgactac tatcaaaaat cctataagtg cgtttgtaag agccgttcta
    42961 gcagaggccg ctttttttgt atcccctttt gctgtgatat gttctatacc cgcaatgccc
    43021 aagaagataa ctgttagagc aatgccgact cctataacta agttcatgac aacgccaaat
    43081 atagccgcca gagtacccgc ggttgctatt ggtgatcctg aaaacgggtc aacgacagta
    43141 acaggaacgc tagtttgtgc atgagctgtc tttaacattt tttctactaa catttactgc
    43201 acctccttta taaaaaaacc tgaaaagtaa cattttacta actgtaatat tatatcagat
    43261 gaactggatt gttagtattg tatttagaaa tcaacttaat acttgcagga tatgtattat
    43321 taacacttgt tatatatggc ttgtggtatg gtttgctgga gatcgtttgt gttactatat
    43381 aagcagtaaa tatttttcat agttgccttt tgctaaatta caaagttaca aaaagcaaag
    43441 cagctctgat ggagtagtct tagcgctggt acagggggca ccctatacaa gacaaaaatt
    43501 atcatgtcgt tctttttagc actgcttgtt atattggtaa tacttatagt ccctttttta
    43561 aaagttatac ccgaaggtta tcacggcgta gttctttttt tggggaaata taacagaaca
    43621 cttaaccccg gcttaaattt tgtacttttt cccctcgaac aagttataaa ggtcgacata
    43681 agagagcagg ttatggacat tcctactcag gaggctataa ccaaagataa tgtttcctgc
    43741 atggtagatg ggatagtacg ctttaaggtt ttaaatcctt cgacagctat atttaaagtt
    43801 caaaactatc aaagtcaaat atcggccaaa gctcagacag ccctaaggga cgttataggc
    43861 agcattactt tagacgaggt acttacaaaa agagcagaaa tcgcagaaca tataaagggt
    43921 attgttgaca cggcagccga acagtggggt gtggatatat cggcaattga gatgcaaaat
    43981 attgagattc ctgcagatat gaaaagagct atggctcaac aagccgaggc cgaaagggat
    44041 aaaaaagcta gaatcattaa agctcaggga gagaaagaag ccgcagtgca gttggctgaa
    44101 gccgcgcagg ttttatcggg agcaaaaggt gctttggagc ttcgaacact gcaaactcta
    44161 aaggaaattt cagcagatcc ttctgagaag ataataatga ctatacccaa ttcgtttttc
    44221 gaggtgttaa atccacgaaa atagttatta gatgaataga ggtaagatcc agatagcttt
    44281 taattatacc ttcaacgatt ttgctaaggt aattcgctat attccaaaaa acccaaacat
    44341 tattttggaa gctggaacac cgctaataaa gtacgaagga ataaaagtta tatccaaaat
    44401 gcgctcgctg tggtcaggag agatctgtgc tgacatgaag atagccgatg gcgctcatga
    44461 agaagtgctc atggccaaac aagccggtgc aactagtgtt actgttctgg gcaattcatc
    44521 tgttgaaact ttaaaaatat ttgtcgatag ctgtacaaaa ctgaatataa cctcaattgt
    44581 tgatatgatt tctaacgaaa agccccttaa gactttgtgg aaagcgaatg tggttcccga
    44641 aatggtttac atccatcgtg ggagagatga agagaattct tttgggaaaa tcttgcagta
    44701 caaagacatc tcaatgttga aaggtaagtg ggatcttaag gccggcgcgg caggaggaat
    44761 ggataagaga gagctacagt cggctatttt taacagcgct gatgttgttg ttgtaaacat
    44821 cgtccggccg tcggatagtt ggaaaggtat agtttttgat agccagttcc gaaccaacct
    44881 agaagatttc ttgcgctttg tagcatagcg gtaaagtatt ttagataggg tagtcttagt
    44941 ttgcacaacc tcgcctaacc ttaagtagaa actaaagagc ccccagacct ataaatgcta
    45001 taatagttga tatgcaacgt cctgccattc atgctcgaaa gttattttta atattgttgc
    45061 cttttttact tctaactacc gttttgtctt atagcgcctt cgccgactcc tgcaaaaaca
    45121 ttgaagattt agatgacaga gagaagtgtt acgcaaaaga gtacgaatca actgaaaaga
    45181 agctgcagga tgtacgagag cagaaagata gcgtatcaaa aaagattagc gacttgtcct
    45241 ctgcgctgac tgtaaaaaat gaagaaattg ttgaattgca gaatgaaatt gctgcgatga
    45301 acagcgtgtt gaatcagata aaccagactt tagaagataa aaatctgcag atggcaaaaa
    45361 aaatcgagtt gcgtaacggc atgttgcgta ctttcgctaa aaaaggcaac ctctcaacca
    45421 ctgattttga attaattttt ggtaccgcgg ctgtttctga aaatcttacc gggtttcaat
    45481 atgcaacgct taattatatt tttgaaaaat ccttgaactc agatgctata aggaccatta
    45541 aggttcttca gaccgagatt caaaattttg agcgcgataa aaaagaggca gaagatttga
    45601 agcttgagct tgagtcagcg caatctaatt tgttggtttt aaaaaatagc ctttcatcag
    45661 agaaagcaag cgccgaggga cagtacagcg agttaagcga taaagaaaaa tcttacagtc
    45721 agaaactgtc ggatatttct gcaaaacaac aggaaatatt aaatttaaag gcgggaggag
    45781 acaacgggac agtaggagac tatgattctc caagctggaa ggtgccggaa cctcctttta
    45841 gccccgcctt tggtgcgttt agctatggtg cttacaccca ctataaaggc atgagtcagt
    45901 acggagcgaa aggaagagct gaagcgggcc aagattataa agatattatt aagttttatt
    45961 acaaacaaga cgtaaaagag aaggatgatt ttccaagcaa aatctgtgtt caaggctacg
    46021 gtgatttaga ttttcaatac tatttatacg gcttggccga aatgcccagc gattggccgg
    46081 aagatgccct aaaagcccag gcaatagccg caagaaccta tgcttataga tattggaaaa
    46141 gcgggcagtg tatttgtact acgcaaagct gccaagtatt tttaaaaagt aaggctgaca
    46201 acgttccgtc tcgctggaaa gaggccgttg atgacacaaa gaagaaaatt attgacggcg
    46261 atactaccgc tatgtacgca tcaacctccg gaggttacgt tgaacccgga ggatgggaca
    46321 cttccggcag ttggccggga ggggcctatg aaaaaaaggc caaatctcct tggttttact
    46381 gggcctggta ttctcagaat tatagatttg attctagtac atgcggaagg tcgcatccat
    46441 ggttaaccga gaaagaaatg gccgatatac taaattcgtg ggtagtttgg gacaaaggca
    46501 gcaacagcga cagagataga atatcccctg taacaacagg ttgttgggga ggaaaccctt
    46561 actcattaga tcaaatggca agtaaggccg atgactacgg caccaagtat agcaagataa
    46621 atagcgtaga tgttactgtt ggaaataacg gtgtgacaac acaagttaaa tttagtactg
    46681 acaaaggcaa cgtcacaatc tccggtgatg tttttaaaac cgtctttaat cttagagctc
    46741 ccggatatat ttctttaaga agcaggcttt atgacctaca aagagagtag cataacttga
    46801 gaagagtttt ttgaactata gttcaggctt aggtcgacag gcgcatatgc gttatttttt
    46861 tttgctgtta gattagtgcc gccagtgtta ccagctgctg cacgaattct aaagaagtcc
    46921 gcgcacactt agcaacggta taggggatct attggtacaa aacttagaac ctcacattta
    46981 ttctttgacg ttctttactg cagactggaa gcctgctcgt gtcttagcgg ttcttaaatt
    47041 gttaacccca aaagagactg aattgctagc ctgattatta ctataaatcc aagcagcgct
    47101 atagttccaa gaacagcagc tacaaagccc tctctagcgt cttttacttt tgcgggatct
    47161 ccttggctgg tcatggtttt atatccattt attagtatca tcggcaagct gaaaaaacca
    47221 agagccgcag gaatcataag tgacataaga gatgtcagca aagtttcaaa gtttatcaaa
    47281 ggccctgtgt attctcttct tggcggggcg ggtatgcagg taatgttccg aacgcattga
    47341 ccgctggtgt tgcaagtttc atttgctgcg cagccaccat aaccgcatgt atttatgtac
    47401 gctgggtcat atgtacattc gcccgagggt aagatacctg tcggtatccc gcatatatca
    47461 gtaaatccgt ttccatctcc atcgacgtcc aagacgcatc caggtctagg aggaacacct
    47521 gtgtacccgc agtattcccc agtagtgcag gcaactttga ctggtccgca caggtcacgc
    47581 ccattgtggt ttgcaatgaa agttgcgtcg catgcaggtg cagtagatgc aaggtaacat
    47641 gttgttggag cgctgaaagg ctgactaagg tttatcgaaa accttgccgc ctcaacaacg
    47701 ttgctgtcat tggaaaccca attgtagttt cctgctactt caacgaggtt tacttgaaca
    47761 atcacttggc tagtggcggg gtcagatatt tctactcctg taccaaaaag ccgagttgaa
    47821 cttgatggct cacagcccgg gccagaaatc gcgccgtcct cttggcattg gaatacataa
    47881 cctgaactat ccgctggcca ttcaatctcg ttcgataccc ctattcttac accgttctga
    47941 tcgtacacat gcaagttaaa acgtaccgtg tctacatgga caaaagagta atagcctgat
    48001 gggtctgtag aagcaggtat tttagggttt attgttaccg tggcgtttac tatactattc
    48061 aaagccatgc taccgcagtt ggcaacttca gaggagaaag aatccattaa aaattcgtag
    48121 ttcgttggtt catgtgttcc ttctgcatgc gcagttttca caaatgctct cgttaaaaca
    48181 tcgttgtcga tagggatacc cactgcagac actgcccaga ttactcctgc aacaaaggta
    48241 taaaagaata ttattttgat tctgttcatt attgttatct ttataactgt gggtacatta
    48301 caagtgtaac atgggcagaa aaggctttca acttttatct tctatgttag gccgaatagg
    48361 ttttacacgc aaactgatca taattacaga attgtttcat ctgttgctaa gccatgcttg
    48421 gcactgtagg aaatattttt ttctaaaaat taaccacctt ttacatgtat tctaaatgga
    48481 attcttttta catgatgtta caataagcct atgcaagtat ttttgaatac agatggggga
    48541 gctaggggta atccggggca tggggccgca ggaatagtta tttttgaaaa cgacgctgtt
    48601 aagcacacaa gaaaaaaata tttgggaatt tgcactaaca atgaggctga gtatttagcc
    48661 ctgattttag gtttggaaga ggctattaaa catgggtata aaaatattgt ctgcagatta
    48721 gacagcgagc tagtagtaaa acagctcaca ggagtttata aagttaaaac ccctcacata
    48781 aaaattttac atgacaaagc agccactcta gtaaagaagt tttctacagt aattttcact
    48841 catgtcccga gatctcaaaa cagcggtgcc gattctttgg tgaacgaggc gattgacgag
    48901 tatttgtcgt cagaagttcg gaaaagttat tagcttttgc ttatgtctta cagactacca
    48961 agaaacgcaa ccgcgcttga acaggcgatc ataaacgagt taaaatatag agctgttttt
    49021 aatgctagcc ttacatatta tcagctctgg aattcactat ttttattaca tcctataaat
    49081 gagagccagc agaaagaatt tacgctggaa aactttaaca aggtgttaaa gaagttaaaa
    49141 cagagcaagt acataaatcg cacaactacg ggaaattact atattcagac aaaaaaacca
    49201 ttcaagtggg ggcacagggc aaactacgct caagaatatt taaatagttt aggcccagtt
    49261 gttaaagtct tgtcacatat aggctggatc aagctgctcg ccgtttctgg atctgttgct
    49321 gcatacagtg ccaaaaaaaa cgatgatgta gatttattta tagtcacaga aaaaaaacgc
    49381 ttgtggctca cccgtttgtt cgttgtttta ttattaaaga ctctgcggtc ctataggaca
    49441 gataagaatt ccgcaaacaa aatctgccca aacatttttt tagatgaagg tgctctaact
    49501 tggcccacag agagacaaaa tatctatgtt ggacatgaga ttattttgtt aaggccttta
    49561 ataaatcgcg ataatacgta ttttaaattt ttggatgcaa attcctggat aaaaggtttt
    49621 ttctgcactt tttgttacgc ctcggaaaag acctacaaat ccatttataa gcctggcagc
    49681 aagctagtag atttactgga agattttgta aaaatggtcc agcttaggta tatgaaacaa
    49741 aaaataacct cggaaataat acaagaccgg ctcttgcatt ttaacaaaca cgaccacacc
    49801 tcaaaaatta ttaatgaatt tgaaaaaaat taattaattt ataatttcgt tactcaaacc
    49861 tacctacgca agatagtaga tgtagtttac ccaacatgtt gagctctgca aggcaaagaa
    49921 attacagcaa gccacctgcg taagctggtg atagttgata atttcgttac tcaaacctat
    49981 ttatgcaaca gaacttcagt ttttaactta caatcgaacg agcacgtctt tattagtaac
    50041 gagaggctgt agatttagct cgcagccctt gattttgttg taaaaccttt tttagttgtt
    50101 ttttatgcct tcattgcttt ttctaaaggt tgtgtataaa aaagaagcac ctccttttgg
    50161 aggtgctttt gggccggtta cgctacaaac atattagcat tttttattta ctgcgataag
    50221 acttcaattc tcagttcaca gatgaaagca catgtgtgtg gtattaaaaa agatgctgcg
    50281 gacataagtc cgcagcatcc tcattttcca gcggtaaggt cgctggcatt gtccgcgggt
    50341 taaacggaaa tgatctacat cattcccggc attccaccca ttccgcccat tccaccggcg
    50401 ctcgagttct cgtctttttc ttgtggagca tctgttacta agcactcagt agttagaatc
    50461 atggttgcaa cagatacggc attttgaatt gcgcttctcg ttacttttgc aggatccgtt
    50521 attccttctt ctatcatatc gacgtacttc attgtcatta cgttaaatcc catgtttgaa
    50581 tttttcttct cggttgcaag cctttcaatc tcacctatta ttcgtcctgc atcaactcct
    50641 gcgtttgaaa caataagcct taccggtttt tcaagagcgt tatataaagt ttttgctccg
    50701 acttcctcat cttcttcaag cttcatatct aataaagctt ttctaatctt aagcaaggtt
    50761 actccgcctc cgggaacaat cccttcctct attgctgctt tggttgcatt tacggcatct
    50821 tcaacgcgta atttcttttc tttaagttcg gtttctgaag ccgcgccaac tttaataaca
    50881 gccacgcctc cggttagttt agctaatctc tcttgtagtt tttctttgtc gtagtcagaa
    50941 gtggtatttt ttatttggct ttctatctgc tggacccttt cttttatagc tacttcgttt
    51001 cctgtaccgc cgactattgt ggtttcatct tttgttgcaa atactctttc tgctcttccc
    51061 aaatcctcga ctgttactga atccagcttt cttcccatat cttggctaat cattgtggct
    51121 ccggttaaag cagcaatatc ctcaagcatt gcttttcttc tgtcaccaaa tccgggggct
    51181 ttgacagcaa gtgcgttaaa ggtgcctcta attttgttta cgactaaagt tgctaaagct
    51241 tctccgtcga tatcatctgc gattataacc agatttttcg aaacttttac aaagttctca
    51301 agcattggta gcaagtcttg catacttgaa acttttgcat cagtaacaag aagatacgca
    51361 tcgtttattt cagcggtcat cttatctgta tcagtaacaa aatatggaga tgcatagcct
    51421 ttatcaaact gcattccttc tttgtgatct agctccattt cgaatccttt tgattcttca
    51481 actgtgataa caccgttgtc tccaacttta tccattgcgt cggcaattaa gtttcctatc
    51541 tcagaacttt gtgctgagat tgttgcaacc tgggcttttt cttcttttgt tgttattttc
    51601 tttgctaaag aggctatttc tttaactaca acatctgtag ccttttcaag gccttttctc
    51661 agaatcattg gatttgcgcc tgcagaaaca tttctaatac cttcttcaac taaagcttga
    51721 gcaattactg tagctgttgt agtgccatct cccgctatgt cgtttgtttt tgaagaagcc
    51781 tcttttacta attgggctcc catattttca aacttgtcct gaagttctat ctcttttgct
    51841 actgttactc catcgtgaac aacctgaggg gacccccagc ttttatccaa cgctacgttt
    51901 ctaccttttg ggccaagagt tgtagctaca gcttgcgcca aagcatctac tcctgccttt
    51961 agttttgttc gggcatcatt cccgtaaatt atttgttttg ctgacatatt gtcctcctaa
    52021 tttatatact cttttaactt tttattactc gaccacggcg agaacgtcat cctgctttac
    52081 taggagatgt tctttaccgt ttacttttac ttcagtaccg ccccatttct tgtacattac
    52141 tatagctcca acttcgacct ccatttttat tagatctccg ttttctcctc tttttcctgc
    52201 tcctgctgct ataactctgc ctttttgtgg cttttctttt gctgtgtcag gaataacaat
    52261 acctgaggga gttgtagtct cttcctctaa gggttcaatt aatatatagt cgaacattgg
    52321 ctttatttta gtatcggcca ttctaacctc ctgttttatc gtggctttat tttttaattt
    52381 tataaagctt tgatataaga gattttgcaa tgtcattgca aaatctcgct tgtgaaattt
    52441 aatactcctc gcgtatagca gaaaatttat agcactctat gcttgtgagt gtcaagtttt
    52501 agaatgcttt tttatttagc gtaaattctt attttatctc cgccaatctt ttcttgtttg
    52561 tatatttcta ttcgaccgat ttcttttgtg ttttgtacat gcgggccgcc gcaaaactct
    52621 ctactaaaag cactttcaag cgaatcccct ataaagtaaa cttttaccat gtctccgtat
    52681 ttttctccaa aaaaatgcag tgctccggtt ttttgtgcag tttccttggg cataatctca
    52741 tactgaacct ttatagcctc ttctatcttt ttattcacta gctcttcaac agccttaagt
    52801 tctttttcag ataacttctg gttgtgcgag aaatcaaatc ttaaacgctc gtttgttata
    52861 ttactgccct gttgggtaat ctgtggccca agagtttgtc tcagcgccca gtgcagtagg
    52921 tgagtggcag tgtggtattt agtaatttgt tcagtgtgtt ctgctaagcc ccctttaaat
    52981 ttcttctctg cgccgactct cgacaaagct tggtgttctt gctgaattcg tttgaactct
    53041 tcttcaaagt ccttttgatc cagattcacg cctttttctt tggcatcttc aataaagatt
    53101 tcttgcggat agcccactga ctgatatagg gcaaaactaa tttctgccaa atgttgtgtt
    53161 gtggttgtgc cccctatgcc tgaaagttct ttttcggctt ctttcgatcc tttatgcagg
    53221 gtttttctaa attttgtttc ttcgtcacgc agaattttta atgcctcccc ctctattttt
    53281 ggaagttccg ggtatatcca actaagcatg ttgatagttg gggaaagaag tttggatgag
    53341 atatcttggt caatatctaa gaatcttccg gcgcgaacca ttagtcttat tagtcttctt
    53401 agtatatacc cttgatcttt attagatggc agaactccgt ccatcattag tatggtggct
    53461 gctctcatgt gatcggcaat tttcctcatg gcgatagtag tattgttgtc cagcttgtat
    53521 ggttttccgg ttattctctc aagctcacta attattggcc aaaaattatc ggtttcaaaa
    53581 atatcgtttt tttcttgagc tacaagggcc agtctttcaa ggccgccgcc aaaatcaaca
    53641 tttttttgcg gtaattcgac ccaactatct tcggcttttt tatattgcat aaaaacacta
    53701 ttgccgattt ccaagaactc atcttcgttt tcatgcgggc tcaatccaaa tcccgtaccc
    53761 tcccgtccta tgtagtaaaa aatttcgctg tccggacctc caagttcgcc tactgcttct
    53821 cctctttgcc accaattgtc ttttttacca tacgcaaaaa ttctctcatc ctcttttgct
    53881 tcaatgccgt atttttcaaa tattctttta agtatttcaa ccgcctcagt gtcctttgga
    53941 gcgtcttcgt cgcccttaaa aactgtggcg tatattttat ttggatccag gcctaaaacc
    54001 tcaattaaaa actcgtacgc ccacggtaat tgctcttgtt taaaataatc ccctaaactc
    54061 cagttaccta ccatatgaaa tagagttgta tgtctatttg tgtgcccaat attctcaata
    54121 tcttcaaaga accttaaaca cctttgaaca tttgtaagtc tttttcccat ggggtggctc
    54181 tgccccgata agtagggaac aagcggaaac attcccgagt tcacatacag gagtgtaggg
    54241 tcattttccg gcacaataga aacgtttggg attagctcgt gcccgcggtc tttataaaat
    54301 tgtatatact tttccagtat ttcttttgaa gtcatgtttc tttggaggcc tgggcgggaa
    54361 tcgaacccgc gatgagggtt ttgcagaccc ttgccttacc acttggctac caggccacga
    54421 atttttgtgt caatgctagt tgctatttta gcacgtgcgt gctatctttc ctatgtatat
    54481 attatgtcaa tttcaacttt agtaccaaca aaagagagaa aaacatcaaa gccattctac
    54541 actattatcc tgtacatttt aatttctttt ggttttgttg ctttggcata ttttgggctt
    54601 gtttttgtta aaaatatttc taaatctaag atacctgcag cccttgaatt gaaagtagaa
    54661 aacggcgagg cagaggtttt tctggacgga gtatctattg gaaaatctcc tattgagaat
    54721 tatcagcttg aagccggcag tcacgaagta atgctgcagt cgggcgatgg catgtcgtat
    54781 aaaacaaaca tcgattttgt tccgggggcg gccgctgtag tcatcaaaga gctaggttct
    54841 tccagtctgt tttcctcagg cgccagtgtc tggtttgcta aaaagtctgc agacagttct
    54901 ttgagtgtga tatctgagcc ctcgggggca gatgtttata tagacggcac tctggtggga
    54961 agcacgcctt acaattcggc taagttaagt gccggaggct acgagattaa ggttgcaatg
    55021 gctggatacg aggaccgtgt tcttcgagct gaggttcttg aaactcacac gactaacttt
    55081 aacgttattt tattcccaaa tgccgtacct tctgaagtag gcactataga aggttacgac
    55141 aatatttacg atgtaacctc aaatgacccc agagttttat ctgatatctc aagctggata
    55201 aaagccatta attactggac aactactaga ggggttaata ttaaagactt agggctgaca
    55261 aaagagccgt tgttcactta ttttattgac gctgagggga tagtatacgg cactgatggc
    55321 acgactcttt cggccgatca agtggccgga ttgtcctcgg atcaaaaggg tgcgtatctc
    55381 cacagtaaaa atgtttctga aactgaagca tcccagctca cagagaaagc aaaatcatca
    55441 gttgaaaaat taggcacagg cccggttctt actgaagctg aaaataaaga aatccctgga
    55501 actgaaaatg ctggaacggt cgggacgccc caaacaggca tagggggaca ggcgacaatc
    55561 cttgatactg gcttgggctg gcttagggtt agaaatgctc cgggtttgtc tggtgaagag
    55621 atagctaagg taaatgtcgg agaagcgttt gaggttcttg atattcaggg agattgggta
    55681 aaaattagag ttgatgcaaa tgttgcggga tgggtttctg caacctatgt ttctatagat
    55741 taaaccctta taattttttt actaaactac cttgcataag ccagtagatg tggtttactc
    55801 aacatgttgg gcttcgcggc gcaaataaat tacagcaaac catttgcgta agctggcggc
    55861 ggctgatata agcttctatt taagtaggtc ggaattccac tgtaaaatat caggcctaag
    55921 agtttcaatt cgcacaaggc tttctttaaa tttttcctcc agccccagta gattttgata
    55981 ttttgctgct tgtatttgaa ctcccaggtc tgtggggtag tctgtttgaa gtttctctga
    56041 ataatctaaa aaatccccaa tagttgcatt atcgatatta tctttgcttg acggcatggc
    56101 tagatcatta atggccaaga aataataaag gcccaacgcg tctctttttg tagcgagatt
    56161 gttcgggttt attttaatgc ctttttcaat gttggcgatt gcttgttttt taagctgcgc
    56221 gtgcatatta ggtttagtgt ctagtgtttg aagcgttaag gctttagctt tctgtagata
    56281 gtaatttggc tcatatttcc acaatctgag agcaacgtct atgttttcgt ttgacgcaga
    56341 caccgagccg tttaatatcg cgtttctggc tttactataa aagtattctg cggctgcata
    56401 tcttaaagca aacactatta tgagtacaaa aattccaagt gtacaaagct tgctcagttt
    56461 gtgcaccggg ccatttaata ttctatttaa tgactttatt cttgctagcg tttctctgct
    56521 gttctgtgtt ttttgctcga acttcataag aagcttgccg tagttattta gttcatcttc
    56581 tccttctctc gtctctttta aagcgtaaat tccaacgata acaataaaaa taaatgaaag
    56641 agctatactc ggccagccaa aaaagtttgt tataactata gtcgaaagag caggtgacag
    56701 tatttcatct tttcttctat ataaataaga taccagcaat atgtagccga taagacctaa
    56761 aataccttgc tctgccaaca tttccagata gtagttatgc ggtttgttta tgatgtagtc
    56821 ccattcggac gaaaaattca atgctggggg cctaaagttt tgaaaaacaa acggaaacgt
    56881 ttcaggaccg tatcctaaaa taaatcgagc cggacttgat gttgcaagct ggattgtccc
    56941 gagccacacc tgccctctta taaaagcagg atcggaatag aatctttggt ttgtgttcgg
    57001 aaaagtgtct ggcgcgttac ttttaatgtt gtttgtagct ctgttgctgt actgtgggtt
    57061 ttgcaaagcg tagttagttc ccgaaaagct tgtttttatg tcttcaaata cgtcgtggac
    57121 cctttttgct aaaggggccg cacagaccgt ggagaataca aagaaaaata ttaaagtgat
    57181 tctaagcttc ttacactttg ctattattat gtttgttgtt ttatggatat ttttcctcga
    57241 gctacctagc ttcgcgccga aaaatattaa cactaaaaga cctataaaca agcccgcaag
    57301 cgcggacaaa gagtaggtta aaacaatgac tgagaatcca aggatggcgt ttaagttata
    57361 ccaaaaaacg cgtcctttta tttgctcttt atttgtttgt atcaatagtg ctaggtttag
    57421 cagtattgtg gttgctaaat acgatccgag ccaattaggc tgtccaaagg ttcctccaat
    57481 tctaaaagtt tgggtagaga tatattgcac caaagcaaaa atacttactg atagagtcga
    57541 gaaggcgatg gtttctatca attcttgcga ttttttagtg tcaatttttc taataagaag
    57601 gtaagtagca agcaaaataa tttgaaataa aaaagaatcc ccgtagcgtg tgtagtagcc
    57661 aattaaagat ccaagaaaat aggttgaagc tagagtagat agcgctaaga gaataaaatt
    57721 tcctattact agagcatcgg tctcggaaaa ccctgattta tatgacaatc tgtcggcact
    57781 tgagcgattg ttctgctttt gtgccataaa agctaggcta acagctatta acgtgaaaat
    57841 aaattttgag aactcgtatg tttcttgacc cggcaaccct aaaattatag gcaggagtaa
    57901 aataataagt ttaattgacg aagtagtcga cacagctgta ttatactatg taaggagtaa
    57961 gttctgcctt atgttttcaa agattttaat cagattagtc gaccaagcta tagttcccgc
    58021 aatattactg ttggtgtctc gtgttacctc aatgttgcta ctttctgcaa tattcaatgt
    58081 tccttatgag cttaccaaca aaggattcac tttcttagaa gtttccgatt atgtaaggat
    58141 aaactcttat tccatgctat ttatgctagt ttgtgttttt gttggcttac tctacatcat
    58201 gttgaagtca tttatttttc atgacacaca cattaatcct gttttagctg caaggttatt
    58261 tactctcagg ctggattcct tcattcaagc gtcttttgat ctttattctc agggggcggt
    58321 gtggctgtcg tatttatatt tgctaactgt tgtcattata gcaatggttt ttaccggaat
    58381 gctttatagt tggattttcg tggttgcttt tattatgtcc attgtcgcaa ctgtccttct
    58441 tgtatttgac attgaaaatg agcttgaata tacaaaagat tctaaagagg aacaagcaga
    58501 ttctttagat gagtatgttt tggaaatgga ggtttagcgt ggatgagtac caaggcttca
    58561 aatcaaagca cttactgagg ggactaactg acggcctctt aatagctatg tttttaggtc
    58621 ttctcgcaat gcccatttcg gcaattggct tgtttgctta tcaaaattct agtttctctt
    58681 ctgttgcttc aaacactaaa tcgcaggacg acattctctc ccagcagccc gttatagagc
    58741 cggcctctcc ctctgtggcg gttctaggtg cccagagtga ccctctatta actgacctaa
    58801 ctcttgagga gcagaagctc gtcgaggcct atatagaata cctgaagctt agccgcccgc
    58861 ggggtggtag cgttcttgac cttcaaaata ttccaagcaa ctaatgttca gagaagtatt
    58921 ttttaaaaat gtttttctca gttcacaaaa ataatttttg tatataatcc tagatatttt
    58981 tatagggttg agaaatatat gagtattgaa ggtagaaggt agatcacaaa caaagtagcc
    59041 acgccccatg ttattacaga agcgaacagc ggtttatcgg acatgagtac tcttgcggga
    59101 gattctccct cctccttttc ataaatgacg tacagatacc tagcgacgcc gtaaataaca
    59161 ataggtatag tgagcatcat ccattttggc gagctgatta ttgatggtag aactttagtt
    59221 attgcagcgt cgttcgtttc cggagatatt tgaaaagcaa atagagcgta ggtaattatg
    59281 cagaatgtag aggacatcga gatcatgcta tttaagaggt tgtcggggta gtgccttagg
    59341 gtgtcgcgag ttttaatttg gtcttttgtg tctaaatgtt taagaagtat ggttttttca
    59401 gatcttcttt ttccaaacgc caggagaagc gaaaggccag ttgttgtaag aattagccac
    59461 gaagacaccg agctttcggc cgcaaaacct cctgcaaaaa ctcttattat gaaccctcct
    59521 gctacaaaaa gagcttccaa gattataata tttcttatct tgatagaata ggcaacctgt
    59581 attgcgatat aaaaaattac tgccaatgta aaatacgtgc cgacaaagaa atatcccccc
    59641 aacaccgacc ctcctagaag aataaatatt attatttgag cttgtttggc agatattttt
    59701 cctgaggcaa tggccctgtt tcttttaata gggtgtaggc ggtcttttgg ggcgtccata
    59761 aggtcattaa aaaaatagat gccagaggaa agtatacaaa aaaccaccac acctttgaca
    59821 gtgttagcaa aaacattagt ttcaaagagt gtgcctgcaa aaacaggtgc ggcaaaaaga
    59881 cttaagtttt taagccattg tcgtggcctt gctgttctga ttacgtgata aataaaccct
    59941 accattactg taaatagtat gtctttttgc ccttattttc aatggtttct ttgatagagc
    60001 caagcagtaa acctttgtcg cgcccgattt tttcattgct ttcgccgttt ccaataatgt
    60061 ggctcctgtt gtccaaatgt cgtctacaag tattatttcc ttgcctctag agtcagcctt
    60121 agcactaaag gcatcttgca catttttgta tctgtttctt ctcgttaggc catattgatg
    60181 gtacgtatct atgtttcttt ttagcaagcc tgaatttatt tcaagtttga aatgcttcgc
    60241 aaaaatttct gcaattagtt ccgcttgatt aaagcctctt tttttgagtt tagactttga
    60301 gggcggtacc ggaactatta ttggagcact gttatactca aagagatctc taacccccga
    60361 ctgtaggaga ttattagccg cattagtgct gatttccttt aatatctcga aatgttttga
    60421 accgaatttt gcttctttaa tacagagcct gacgaaacct gcgtattcgt aaattcctaa
    60481 cgtggcttcc ggagaatact cggaagcgca taatttatgt gttttgccat cgatagtagg
    60541 cttgtcgcac acaatacagt tcgagttatt taagactttg aaccaggcta ggcagtcgct
    60601 acataataag gcgttttcgg acttggctat agcatctttt agcacaaact taggcccata
    60661 tacctttttt agtacagcgt gccttgtttt gcagagcacg cagcttttcg gaaaaattag
    60721 gttgtaaaga ttttccataa gttatccaca atttaataaa ccccgtcact aaaggtcggg
    60781 tatagtacga caccctatag cgtgtgtgct ttgtacctta tagtacacat atcctagcac
    60841 ctcgcctcgg cctgtataag gtggttgtct attttgggta gccatgtatc tagagtattc
    60901 agcagtcgag gcccccgtgg ttttaaacga agcttttcac tatatattct ggctaaatat
    60961 taagcgcgaa tcttataatt tcgttactta aaactactta attaagctag t
//
LOCUS       DAOSWN010000002        25970 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_1204_length_25970_cov_4.332279, whole genome shotgun sequence.
ACCESSION   DAOSWN010000002 DAOSWN010000000
VERSION     DAOSWN010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 25970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25970
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1204_length_25970_cov_4.332279"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PLF29_00300"
     CDS             <1..260
                     /locus_tag="PLF29_00300"
                     /inference="COORDINATES: protein motif:HMM:NF017042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HXK52417.1"
                     /translation="RRICDSDGKTYNIYTLPPEEVAKCKGNLIQRADDTEESVNKRLE
                     IYYSDTMKVVDHFRALGKVFEVDAGGSPAEVAELVIKVLQD"
     gene            262..1005
                     /gene="map"
                     /locus_tag="PLF29_00305"
     CDS             262..1005
                     /gene="map"
                     /locus_tag="PLF29_00305"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HXK52418.1"
                     /translation="MGYSKSNSDIEKMTRCGRISAGAMSAVLSNVRAGVSLIELDSVA
                     ADYISGSGAKASFMTVDNYRFTTCINVNEGIVHGLPNDYRLKDGDIVSIDLGALYLGF
                     HTDLSHTVEVGTSKESEFLSAGKEGLEKAISKAVEGNRLGDISFEMQSAVEKHGWSVS
                     RDLVGHGIGKQLHEDPYVPCYGKPGKGMKLKENMVLAIEIIYQKGKPELALERDDWTY
                     RTLDGSLSGLFEHTVVVGKTEPKILTVFN"
     gene            1055..1273
                     /gene="infA"
                     /locus_tag="PLF29_00310"
     CDS             1055..1273
                     /gene="infA"
                     /locus_tag="PLF29_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HXK52419.1"
                     /translation="MNTKEVVIKEGIVKESQTGGLFLIEFGPGEEAWSVLAGKLRKFK
                     IRILPGDKVKVEFSPHDLTRGRITYRFR"
     gene            1274..1387
                     /gene="rpmJ"
                     /locus_tag="PLF29_00315"
     CDS             1274..1387
                     /gene="rpmJ"
                     /locus_tag="PLF29_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HXK52420.1"
                     /translation="MKVRSSVKTICRNCKIIRRSGVLRVICTNPKHKQRQG"
     gene            1389..1775
                     /gene="rpsM"
                     /locus_tag="PLF29_00320"
     CDS             1389..1775
                     /gene="rpsM"
                     /locus_tag="PLF29_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011612501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HXK52421.1"
                     /translation="MARVLGVDIPENKRIEVALTYVRGIGLKKSNEILEATKIDPDIR
                     AKDLSDAQIASIRAHVEKLALPIEGELRRIVTQNIRRLQEIRSYRGERHKKGLPARGQ
                     RTSTNARTRKGKRKTVGGTQKKLTKK"
     gene            1777..2169
                     /gene="rpsK"
                     /locus_tag="PLF29_00325"
     CDS             1777..2169
                     /gene="rpsK"
                     /locus_tag="PLF29_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HXK52422.1"
                     /translation="MAVQKKKKSSKSKSITPSSGRVYILAGFNNTLITFTDDKGNVLF
                     SGSTGKAGFKGSRKSTPFAAIKSTEATAQAAYESGMREVAVLVKGPGMGRIASIKALK
                     SAGLRVTSIADITPIPHNGCRPKNKRRV"
     gene            2181..2783
                     /gene="rpsD"
                     /locus_tag="PLF29_00330"
     CDS             2181..2783
                     /gene="rpsD"
                     /locus_tag="PLF29_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HXK52423.1"
                     /translation="MARYNGPKCRLCRREGEKLYLKGTRCESEKCAMNKKAYAPGQHG
                     NTRGRLSEYGKQLREKQKVKRIYGLLEKQFNTYVQEALRVKGVSGEVLMQQLETRLDT
                     AVYRSGFAVSRAQARQFIRSNIFTVNGKPVNIPSYRVKVGDIIKPVSFDKIQLREGFV
                     MPDWLTANVTERYVKFTRMPTLEDLSENINVQLIIEFYSR"
     gene            2800..3744
                     /locus_tag="PLF29_00335"
     CDS             2800..3744
                     /locus_tag="PLF29_00335"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HXK52424.1"
                     /translation="MLLQPNEIKINKVSEKNSVGVFSFKPLPKGYGNTLGSSIRRVLL
                     TSIKGAAVTQVTFPGITHQFTTIPGVKQDVVDLCLNMKEIVVRSHSDSPVIGTIKKKG
                     KGAVTAGDIKISSEVEVINKDFVIAELADDKTTFEAELIIEQGVGYSIAESRETAKLG
                     AIVIDALFSPVLKVSYEVTPTRKGDVTDLDELIITVQTDGAVKPEEALTEAATLLRNF
                     FSKFASKEEYVEEIIVPASSQSDAAEDTTGNSSSKDDVYLEDLSLPTRTVNALRKHGI
                     EKLSQLAKMTDEEIADVKNLGEKSVKEIIKLLEKENLR"
     gene            3746..4285
                     /gene="rplQ"
                     /locus_tag="PLF29_00340"
     CDS             3746..4285
                     /gene="rplQ"
                     /locus_tag="PLF29_00340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HXK52425.1"
                     /translation="MRHRVNTKKMNRDTDHRKALLRNLSASLILHGKVETTLAKAKFV
                     KPYVEKLVTKARTGNSFDALNKVNAKLRSNEALKKLFSDVAPSFEKRPGGYTRIVKLG
                     YRDGDNAPMARLEFVKTKETKTKDSKAKVTPEKADGKKTIEKKTKGSKVSEKSESEVV
                     VENAMEVIDDSSTNVAKEQ"
     gene            4291..4713
                     /gene="rplM"
                     /locus_tag="PLF29_00345"
     CDS             4291..4713
                     /gene="rplM"
                     /locus_tag="PLF29_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HXK52426.1"
                     /translation="MKILKTTTPKITYIQQEWHFIDVRGRILGDAAQEIAKLLIGKNK
                     AIYTPNINVGDKVVVLNSKKVAVTGRKLDDKTYYRHTGYPGGIRNENLRSLLDRRPNE
                     VIRRAVKNMLPKNKLQKIRMANLYIYEGDQHPHEAQQK"
     gene            4710..5153
                     /gene="rpsI"
                     /locus_tag="PLF29_00350"
     CDS             4710..5153
                     /gene="rpsI"
                     /locus_tag="PLF29_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HXK52427.1"
                     /translation="MKKQQTEVHKEIMKPRKDFISGTGRRKTSVASVFLYKEKGQILI
                     NGMPIAEYFPSEKETLAWTKPFHAIGVSHPEAQFSATIKVSGSGKSAQLGAVVLGISR
                     ALAKIDEEFELNLKKLDLLTRDSRMVERKKPYLRKARKRPQFSKR"
     gene            complement(5213..5494)
                     /locus_tag="PLF29_00355"
     CDS             complement(5213..5494)
                     /locus_tag="PLF29_00355"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXK52428.1"
                     /translation="MLSYKIGKKIKKVRVDNKLSQRRFGEKIGLSAKTISAYECGRIS
                     PPYHVLEQISHTYNAVLVNPPIAKIKFIEKQLEVLEQALQTLKTELRQF"
     gene            5554..6393
                     /gene="radC"
                     /locus_tag="PLF29_00360"
     CDS             5554..6393
                     /gene="radC"
                     /locus_tag="PLF29_00360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HXK52429.1"
                     /translation="MHKNSKLFDRTDLLPREKIETLGSSHLTDAELLAIILRTGVSGV
                     GVIELSTNILSQLGGLRCVINAPKEQLLKVKGLGPAKISSLLALKEMFARLTTVENIR
                     NRKISSPKDVFEVVRSRLMNQEVEHLYLLNLDTRNNLISLDLLTIGTVNQTLVSCREI
                     FRMALLKGAVSIILVHNHPSNDPTPSSEDISLTKILAEFGKGLGINLIDHVVVSDSEF
                     ISIKSMFQLDSAKGQRDDEENFGNKSLHNNQEIRSQDRVHIPINSYNKSFSHKFVYKQ
                     ALL"
     gene            6418..7017
                     /locus_tag="PLF29_00365"
     CDS             6418..7017
                     /locus_tag="PLF29_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52430.1"
                     /translation="MFKKINKKMHASAGVLVLVLLLCSFAGVAYAAFVDKGQVLGSTF
                     SVGSADLKLLRDLSLGADGANLADEIQGPSFSNISSNWTADYLVKLYNNGSSRVTVTT
                     NSDYLTANDPDELRQLIYVEIIEWNDANSDGTADETELGVSYGRDTIVKWKTQGFSLG
                     SIDAGAVKGLVVRFSTGTVSDTKQGAVGIFDFEFNSIGE"
     gene            7116..9671
                     /locus_tag="PLF29_00370"
     CDS             7116..9671
                     /locus_tag="PLF29_00370"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HXK52431.1"
                     /translation="MLFILSQRLKLFLKWYFLDSTKYLFVVGGRALKILNNDFSLLLN
                     LRLLFTPLYGDYTIQGRIFGFMIRPLEVILGFLALTLFLCLYLGTIVLWLCAPVFLFW
                     IYRWEAIIPSILLVLLYLFKYRKTPVSRVGAVDQDKLAYTFQLDALDLCSEILKGASW
                     KDSLKSYRTHYLMKKLEIDYESFLLLLNQYELQETDLIKSSYDIASRMKTRYVEFEQA
                     FYAILGMVVKKEKFLSEFNLRDEQVLECVSWIVGEREKLSAAHFWQLDYRMKSLGGVG
                     KGMTYRVTPFLDSISSDFTNMASKGYFDDIITREDIVNEIASVLDGSRDNVLLVGPAG
                     SGKTSIIKSLSLEVMRGTEYKSLRNNRIVSLDVGSLIAGTSSAGEVAQRIDSVMAEVK
                     GSREVILFIDEIHNIVSGVGGDAGSSVLAFLEPHISTKTVRIIGATETLSYRKYIEPN
                     ASFERQFEVIRMEEASEATALEILKSQARKFELGRGVTITFPALLEAISLSKQYIGGR
                     VLPDSAINVLERGVVSSLSTDRVVNREVIKKEISRVSNVPVTTVSDDESDKLLNLEEV
                     LKKRVIGQDQAISMIVKALQRARVGIRNKNKPIASFLFVGTTGVGKTETAKALATEYF
                     GDEERMIRLDMSEYQEVSSLDKLIGTSDGTKRGLLTDAVKNTPFTLVLLDEIEKAESS
                     VLLTFLQVLDDGRLTDSMGDTVNFTNTIIIATSNSGTKKIQDLVAQRASFDEIQEAGL
                     GAVRDKFAPEFLNRFSGIIVYKPLDRESLRKIALLSLKSVERLAQNKGIKVQYEDGLI
                     DALVDRGTSIEWGARPLNRLIEDTVETYLAKKILSKELSQGDELVLNLEVLNT"
     gene            9707..10708
                     /gene="prfB"
                     /locus_tag="PLF29_00375"
     CDS             9707..10708
                     /gene="prfB"
                     /locus_tag="PLF29_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HXK52432.1"
                     /translation="MEEHKIQLDKLKESLFYDDKKSKIAELELRLQDETLWADWEAGQ
                     KVSQDLAELKRDVEDIELLELYLEEEDYISFENEVKRVEIKTYLSGKHDKDNAILSIH
                     AGQGGTEAMDWTEMLYRMYTRFSERKGWKTSLLSLVKGEEAGYKSVQLELSGKYAYGL
                     LKRESGVHRLVRQSPFNADSLRQTSFALVEVIPYIEEGEEVELSESDITFESFRSGGS
                     GGQNVNKVSTAVRLRHVPTGIVVECQEERTQAKNREKAMKVLRSKVYAIELQRVEDEK
                     AKLKGNYKTPGWGNQIRNYVLHPYKLVKDVRTGIESTNPDAVLDGDLDLFIQEELGL"
     gene            10785..11960
                     /locus_tag="PLF29_00380"
     CDS             10785..11960
                     /locus_tag="PLF29_00380"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HXK52433.1"
                     /translation="MEIDNTQLYSSGLVQPQPKKKIDKKNLTIFIIAFAIPVILGLIG
                     YYVYSVKARNPIDLKSPLSRSQNDGSQKADVMNPLTGVLYTPFAAQEWLDNRPLAVMV
                     NNHVDARPQSGLIYTDVVYEIVAEGGITRFIAFFLTNTPEKIGPVRSARHYFFIPVKE
                     LGDAMIMHIGWSPQAIEAIDTWPVRSLFKGGADFWRDNPRNVATEHTAFVNGKYLREV
                     GNELGWEGKREITPWKFKDETIPPVANEIGLVGEFEPIQVNFWYEGDYTAIWKYNRDT
                     NSYLRFSGFDLEGNPIPLFDQEQTTTQVSAKNLIVLYVTELSIDGDDKNRLDYELVGS
                     GQGVVFIDGTSIPVTWSKASRDDRTMLYDESGNEVVFNRGNFWIMVVPDRNIDQVIY"
     gene            12002..12580
                     /locus_tag="PLF29_00385"
     CDS             12002..12580
                     /locus_tag="PLF29_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52434.1"
                     /translation="MLKDLFVSEVRVKILALMLPNPAESLHVRAIVRAIDTEINAVRR
                     ELSNLYNIGLMKRRQSSNRIYYTVDTSHILYAELLSLVSKEYGLGADIIASRKKLGDI
                     RFAILARRFSRNEPSTMLDIDLFIVGSINLEVLDAIVKNYEQSLGKEIHYSVMGAEEF
                     LFRKRRNDQFISKVLSQGRTMLVGDEEELCSI"
     gene            12582..14240
                     /gene="recJ"
                     /locus_tag="PLF29_00390"
     CDS             12582..14240
                     /gene="recJ"
                     /locus_tag="PLF29_00390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HXK52435.1"
                     /translation="MKRWQIALEQRSDDIIKDLLKIRDIGEDLVESFLNPPKLQEYIR
                     NFDRNFIESLRNAKKAILEAADANVPIIIHGDYDADGVCATALMHNVLKKELGYSSVY
                     TFIPNRFEHGYGLSENSVAAVLGMLIKNGYSPDTKFVLITVDSGITAFNETAQLKKAG
                     HTVIITDHHQKPQNLPEADVIVWNDQLVGASIAWVLGLVLGSKNTSNLALASIATVTD
                     LQPLIGFNRRLVLDGLHVISANCPLGIKKLLEVSGRDSKDIDTYALGWVLGPRLNAGG
                     RLNDAAQALELLTTDDSLAAGEIAGMLADVNKERQDMTFEMYDLASLNFDEASMPKII
                     VSDDERYHEGIIGLVASKLVQKYHRPSIVISTDGDVAKGSVRSVKGINIIEILRNFED
                     LFLSLGGHPMAAGFSIKTENISLLRQKLAEYTEEHILDSQLVPSLEIDLELMPEEISL
                     STNSLLQRLKPFGIGNPEPIFLTKNLVVVSINSFGREQAHISLQLATDDIKIKAKMFN
                     GVEHDIMKRLALGDKIDIVFRMSENFYNGSTYLEVVIVDFRYSV"
     gene            14246..14734
                     /locus_tag="PLF29_00395"
     CDS             14246..14734
                     /locus_tag="PLF29_00395"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HXK52436.1"
                     /translation="MSDKKVIKNSKVLLAIKKLLSESGVEYKMFEHEPVWTSEEASKI
                     RGTPFDSGAKALVLYVDDVPSLFVLPGSLKADFNKIKKLFMAENVRLATPVEVEDLTG
                     LVIGSIPPFGSVMGLHSYFDSKLLEHEVVAFNIGAHTASVIMKPESLMVIEKPKILDF
                     SI"
     gene            14737..15777
                     /locus_tag="PLF29_00400"
     CDS             14737..15777
                     /locus_tag="PLF29_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52437.1"
                     /translation="MFEQLIIIPILIVLSTLKFLDYNYSSNTTYKFSRFGFLLLAIFF
                     AVFVIIPLGGSIGRITDYLLVTNYNSAYNYSFGDKQLFQSSLPKRINNAAVPEISALA
                     YIIVDKNTQTILSEKNSMQKLPPASTTKMMTALTAYDLYDLDSMLLVPATCTRGDNQK
                     AGFQAGDYVSVRDLFFSLLVSSSGDAACALASGFVNTLGGEQLITYDKFIDLMNLKAA
                     SLNLDDTVFSNPIGLDDANGNHVSSAWDLYLLSVELTKNTLFKQIVQTKEYVLYLSNK
                     KVIMTNTNKLLFDVEGTVGIKTGTTAGAGEVLVYEYNKDSTNIVIVVMGSSDRFTDTK
                     NLLEWVISSYKF"
     gene            complement(15844..17019)
                     /locus_tag="PLF29_00405"
     CDS             complement(15844..17019)
                     /locus_tag="PLF29_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52438.1"
                     /translation="MNLVKASVIGKRLTVILAILLVLYILFLITKPAVISAFYYVFPP
                     KDLPNPIFGMLDPLEFVEKPVANNNFSIELNTSNGRLPAGMPTRMKVYEFAPDYFSYE
                     AGRAAQDNAQKLGFTDDELITDLKGDLYSWRSKISGGTLNINKNTKSLELKTPLVGKN
                     IMYKAQTISSGSAAQGAISILTQINRFSDPLYSKGTNTVTFGKIQGNNLTMATNNTEI
                     QFARVDFFRQIETVPILGPNPKIGLINVTLSAAKIQNTEMNYPFMTADVHEIIPNTEA
                     TYPIIPIDEAWAAVVSGKGVVVNVTPKGSSVFDSTTAPRVDRALIENIYMAYYETPEK
                     QQYLQPIYVFDGKYNIGNAPGGEITIYYPAIQGQYIKPLEAIPTTPTPITQPSSPVQ"
     gene            17063..18523
                     /gene="gltX"
                     /locus_tag="PLF29_00410"
     CDS             17063..18523
                     /gene="gltX"
                     /locus_tag="PLF29_00410"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HXK52439.1"
                     /translation="MKMRTRIAPSPTGEFQLGNFRTALYNYALAKNTSGAFVVRVEDT
                     DRNRYVEGSMERTLEVLEKYGIPGDESPLVGGPYEPYIQSQRLELYKEYAKKLLDSGH
                     AYYCFCSKERLEELRDGQRKSGMAATRYDKKCLSLSSEEVQKKLDQGEPYVIRLNVPK
                     DELIKVDDLVYGEITFDSNQIDDQVLIKGDGFPTYHFAVVVDDYIMNITHILRGNDWI
                     PSTPKHILLYRFFGWEDRTPKFIHLPLLKELGSSKKLSKRFGAVAAMDFLKQGYLREA
                     VLNFVMFLGWNPGTEKEIYSLDEFIKDFTIEKIGKTDLVVMDREKLLWYNGHYIRSLS
                     VEDLMLRLAAWAQEHEIELPQADKKYHLEVLSLVHERMKTLSEFVPLTHYFYADPKVD
                     PSLIVSFGGDESARIIKDLLSLYKAVDPKKWTSADLDELSHSYVTNGGYKTKQVFMSL
                     RAAVTSETATPPIFDTLELLGKETTLRRLDSALARL"
     gene            18596..19684
                     /gene="recA"
                     /locus_tag="PLF29_00415"
     CDS             18596..19684
                     /gene="recA"
                     /locus_tag="PLF29_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HXK52440.1"
                     /translation="MPKQNTQTAQSTADKNKDSALQSALAQIEKSYGKGSIMKLGENS
                     PQRNIQVIPTGSIALDIALGLGGLPKGRIVEIYGPESSGKTTLVQHIIAEVQKAGGTA
                     ALIDAEHAFDPVYAERTGVDVNNLLVSQPDTGEQALEIAETLIRSNAVDVIAIDSVAA
                     LTPRAEIEGEMGDSFVALQARLMSQALRKITSAVSKSNCLVIFTNQLRMKIGIMFGNP
                     EVTPGGKALKFYSSVRLEIRRVQALKSGTEDIGIKAKVKVVKNKVAPPFRTAEFDIMF
                     NEGISKEGNLVDVGVEMDIVKKSGSWYEYAGEKLGQGREAAKQFLKENTKVRDEIEKS
                     IRAKMKDSKPLPLELVSGEESEENGIEE"
     gene            19668..20150
                     /locus_tag="PLF29_00420"
     CDS             19668..20150
                     /locus_tag="PLF29_00420"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HXK52441.1"
                     /translation="MESRSRSLEAKIFNKAVAYLALRPHSSYELEKKLSAYWVKISDD
                     VEFDKSTVVIKQAIDRLKNLNYLNDSIYFKDLVNSVANSPNPKSPLELKRYLYDRGVS
                     LEVIESILSDLYTSGLQKKAISTLISKLSGRYSEKQKLVNYLTGKGYNYALIADLIDR
                     "
     gene            20450..22027
                     /gene="rny"
                     /locus_tag="PLF29_00425"
     CDS             20450..22027
                     /gene="rny"
                     /locus_tag="PLF29_00425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HXK52442.1"
                     /translation="MQSIILILAVLSSFGLGIKAYQYYSVKQKKQGIDDSAESKKLEP
                     GLDNQDSSQDSDKPSLVSEARAKAREIILEAKDSSLQIKKEAQDYAMKVKQALELKER
                     ELVKKEAAVEVKEKEIENRQNELKSDREEVQKKLDNLASLESELQSKLAKIASLTREE
                     AREQLLKVFDKELAVEKSKRIREVEEEIKRDSVEKAQEILVDAMRFGATDYVVAHTTS
                     KVKLPDEDIKGRIIGKEGRNIRTFEEYTGVELDLDSSPGEIIVSSFDPIRREIAKVSL
                     ERLIADGRIQPSRIEEIVEKTTKEIEHIMYKEGDNLCHRVGVYNIPKDLVQMLGRFKY
                     RFSYGQNMIEHTLEETRIGVAIANELKVDVEVVKLGCLFHDIGKVVNDEEGTHVQLGV
                     DLLKKHKIDERVINCVAEHHEDRPFSSIESAIVALADHVSGARPGARSEDYEAYVQRM
                     KALEEAAMSFDGVEKSFAISAGRELRIFVTPSKVDDDSTALLAREIAKKIELEQSYPG
                     VVKVTVIRETRFTETAK"
     gene            22359..23141
                     /locus_tag="PLF29_00430"
     CDS             22359..23141
                     /locus_tag="PLF29_00430"
                     /inference="COORDINATES: protein motif:HMM:NF024674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HXK52443.1"
                     /translation="MRILFIGDIVAKPGREIVNNILPEIKVKESIDLVIANVENLSGG
                     KGVTRSTLKEMQDAGVDFFTGGDHIFWQKEVEDCFQEFPLIRPANYPSTCSGEGYKVL
                     DTGRNGSVLIANLIGRTSFGGNQAYTDDPFTLADKILSDTENLDIAFSLIDFHAEATS
                     EKQAFAYYLDGRVGAVCGTHTHVPTCDERVLPSGTMYVTDAGMTGNIDSVLGVKKEII
                     IQQYLTAQNQRFEWENTGKKAFRSVIIDTDSKVISRLDRISD"
     gene            23219..23491
                     /locus_tag="PLF29_00435"
     CDS             23219..23491
                     /locus_tag="PLF29_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HXK52444.1"
                     /translation="MSKQDIVNALAQQGMTKVEAARVLEALTTIITKALAKGEKVTLT
                     GFGTFETRKRAARAGRNPQTGVVIQIPATRIAAFKAGKALRDAVRK"
     gene            complement(23608..24786)
                     /locus_tag="PLF29_00440"
     CDS             complement(23608..24786)
                     /locus_tag="PLF29_00440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK52445.1"
                     /translation="MRSNKTDQFQKVAQEIYKKNLELYNERKRAELLLYKVSEAIYAT
                     DPKGKITLFNHTLEKMLNTTSAEVIGKKASDIISITTEKGEAIDITSFYRMTENFGIP
                     DTAILLSPEGKKYYVHIKVSVIKTGENNIEHLVTMSDITKEKELEKTKDDFVSIASHE
                     LRTPMSIIKSYLWMLSAGKGGKLSKKQAIYVEKSMKSTDRMIALINDMLNISRIEEGR
                     VKIEEKRLNLDLILPDIIEELEIKTAEKDLYLKLENKTNVRYVYGDRQKIREIFVNLV
                     GNAIKFTQTGGITVKIERLKNARHVKFSIIDTGKGISSTDTQMLFKKFQRLENSYKTV
                     AEAGGTGLGLYIVKLYVSKMGGEVGVFSGGPDKGSTFWFTLPTTKQQEDAVSLSTQQP
                     "
     gene            complement(24790..25098)
                     /locus_tag="PLF29_00445"
     CDS             complement(24790..25098)
                     /locus_tag="PLF29_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52446.1"
                     /translation="MTDEQLKRLVLAIIKKQTQIIGENLALERAVNTGLIQISSKDAR
                     NLTIEQIHFSGADNEVVTRLIESYREIFGQASVDVCVNILRENFPNDFRTVIPQTLLV
                     "
     gene            complement(25095..25904)
                     /locus_tag="PLF29_00450"
     CDS             complement(25095..25904)
                     /locus_tag="PLF29_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52447.1"
                     /translation="MKSLPLPKIYIILTIFWIIYWIALQFFSPESRYFYDIFAIVLFA
                     QPLYGSVKAAINSKKWNGRKSAVGRILILISLGLFAWSLGQLAYTIISFFVEEVPYPG
                     LPDIFFIFIDPFYSFAMFNLILVGGIVSNIKKSLVLNVSLLTTTIVALTFTTLYLLFN
                     DLSYFETFDLAIAVDLLYSFGSIVVISLVAINIMFSRLGGKMKVGLYCLLAGFVFQYI
                     GDISYSLLEPLGQAYSASMPDFLFYIATSLVSLAGMMINAELISTTRGERE"
BASE COUNT         8277 a         4403 c         5736 g         7554 t
ORIGIN      
        1 gaagaaggat ctgtgattct gacggtaaaa catacaatat ttatactcta ccccctgaag
       61 aggtggccaa atgtaaaggc aatttgattc aaagggccga tgataccgaa gagtcagtta
      121 ataaaagact agagatatac tattctgaca ctatgaaggt tgtagatcat tttagagctt
      181 taggaaaagt ttttgaagta gatgcggggg gcagtcccgc tgaagttgct gagttagtaa
      241 ttaaagtgct tcaggattag catgggttat agtaaatcaa actctgatat tgagaaaatg
      301 acacgttgcg gaagaatatc tgccggagct atgagcgcgg ttctctcaaa tgtaagggcc
      361 ggagtatcgt tgattgaact ggattctgta gctgctgatt atatttctgg cagcggcgct
      421 aaagcatctt tcatgacagt tgataattac agatttacaa cttgtataaa cgtaaacgag
      481 ggtattgttc atgggttgcc taatgattac cgtcttaaag atggtgatat tgtcagcata
      541 gacctcggag ctctttattt aggctttcac accgatttat ctcacaccgt tgaggtcggt
      601 acgtcaaaag agtcagaatt cctgtcagca ggcaaagagg ggcttgagaa ggctattagc
      661 aaggctgtag agggcaacag gcttggggat atttcatttg aaatgcagtc cgcggttgaa
      721 aagcatgggt ggagcgtttc aagagatttg gttgggcatg gaatcggcaa gcagctccat
      781 gaggacccat atgtgccgtg ctacggaaag cccggaaaag gcatgaaatt aaaggaaaac
      841 atggttttag ccatagaaat tatttatcag aaaggaaagc ccgagttggc tttagagcgg
      901 gatgactgga catataggac tttagatggt agtttatcag ggttgttcga acataccgtt
      961 gttgtcggaa aaacagagcc aaaaatccta acggtattta attgacgcta tagctttatt
     1021 ttgatagaat ttggcgcgtc agtatatttt gtagatgaac actaaagaag tagttataaa
     1081 agaaggaata gtaaaagaat ctcaaacagg ggggcttttt ttgatagaat ttggccccgg
     1141 ggaagaggct tggtctgtac ttgcaggaaa gctaagaaag tttaaaataa gaatattgcc
     1201 tggagataag gtaaaagttg agttttcgcc tcatgatttg acaagaggtc gaattactta
     1261 taggtttaga taaatgaaag ttagatcgtc agtaaaaaca atttgcagaa actgcaaaat
     1321 aataagaagg agcggagtgt taagggttat ttgtacaaac ccaaagcaca agcagagaca
     1381 aggataatat ggctagagta cttggagtag atatacctga aaataaaaga atcgaagtgg
     1441 ctttgactta tgtgagaggc ataggtttaa aaaaaagcaa cgagatcctt gaagccacaa
     1501 aaatagatcc tgatataaga gcgaaagatc tttcggacgc acaaatagct tcgataagag
     1561 cgcatgttga aaagcttgct ctaccaattg aaggcgagtt aagaagaata gtaactcaaa
     1621 acatcagaag gttgcaggaa attaggtcct acagagggga acgccacaag aaaggcttgc
     1681 ccgccagagg acaaagaaca agcactaacg cccgtacaag aaaaggaaaa agaaagacag
     1741 tcggcgggac gcagaaaaag ttaactaaga aataatatgg cagtacagaa aaaaaagaag
     1801 tcatcaaaaa gcaagtcaat tacaccttca tcaggaagag tttatatttt ggcaggtttt
     1861 aataacactt taataacttt tactgatgac aaaggtaatg ttttattctc gggaagtacc
     1921 ggaaaagcag gctttaaagg ctcgagaaaa tctactcctt ttgctgctat taagtctaca
     1981 gaagctactg ctcaggcggc ttacgagagt ggcatgagag aagtagctgt tttagttaaa
     2041 gggccgggaa tgggaaggat tgcttctatt aaggccctaa aaagtgccgg tttaagagta
     2101 acatcaattg cggacatcac cccaattcct cataacgggt gtaggccgaa gaataaaaga
     2161 agagtttaat ttggagaatt atggcaagat ataacggacc aaaatgcaga ttatgtagaa
     2221 gagagggcga aaagctctat ctaaaaggta cccgatgtga atcggagaaa tgtgccatga
     2281 ataagaaagc ttatgcgccg ggtcagcacg gaaacacacg aggaaggtta tccgagtacg
     2341 gaaagcaatt acgagaaaag cagaaagtaa aaagaattta tggattgctg gaaaaacaat
     2401 ttaataccta tgttcaagaa gctctaagag ttaaaggagt ttcaggagag gttttaatgc
     2461 agcagcttga aacaagatta gatacagcag tctacagaag tggttttgca gtttcaagag
     2521 cccaggcaag acagtttatt agatcaaaca tctttactgt gaatggtaaa cctgttaata
     2581 tcccctccta cagggtaaag gttggcgata ttattaagcc cgttagtttt gataagattc
     2641 agctaagaga aggttttgtt atgccggact ggctaacggc aaatgtaacc gaaagatatg
     2701 ttaaattcac cagaatgcct acgctggaag atttatctga aaacataaat gttcagttaa
     2761 ttatagaatt ctattctaga tagtttaaag gagaccgaag tgttattaca accaaatgag
     2821 atcaaaatta ataaagtatc agagaaaaac tctgtaggtg tttttagttt taagccatta
     2881 ccaaaaggtt acggaaacac tcttggaagt agtattcgaa gagttctttt aacttcaata
     2941 aaaggtgcgg cagtaaccca agttacattt ccgggaatca cccaccaatt caccacaatc
     3001 ccaggtgtta aacaggacgt agtggatctt tgcttgaata tgaaggaaat agtcgttaga
     3061 tctcactctg actctcctgt aattgggact ataaagaaaa agggcaaagg tgcagttact
     3121 gcaggagata taaaaatatc atctgaagtt gaagtaataa ataaggattt tgtaatcgca
     3181 gagcttgctg atgataaaac aacttttgaa gccgaactaa tcatagagca aggtgtaggt
     3241 tactcaatag cagaaagtcg tgaaacagcc aaacttggag caattgttat agatgctcta
     3301 ttttctccgg ttctcaaagt ttcatatgag gttaccccaa caagaaaagg ggatgttacc
     3361 gatctggatg aactgattat aaccgttcaa actgatggtg ctgttaagcc ggaagaggca
     3421 ttaacagaag ctgctacact tttaagaaat ttcttttcaa aatttgccag taaagaagaa
     3481 tacgtagaag agatcattgt tcctgcaagt tctcagagcg atgccgctga agataccaca
     3541 ggaaattctt catctaaaga cgatgtatat ttagaagatc tatcacttcc tactagaaca
     3601 gtgaatgcat tgagaaaaca cggtattgaa aaactaagtc agttagcaaa gatgaccgat
     3661 gaggaaatag cggacgttaa gaatttgggt gagaaatcag taaaagagat tataaagtta
     3721 cttgaaaaag aaaatctaag ataatatgag gcaccgtgta aacacaaaaa agatgaatag
     3781 ggatactgac catagaaaag ctttgcttag aaacttgtct gcatccctca ttttgcacgg
     3841 taaggttgaa acaacgcttg caaaagcaaa gtttgttaag ccttatgttg agaagttggt
     3901 aacaaaagcg cgcactggaa actctttcga tgctttaaat aaagtaaatg ctaaactgcg
     3961 atcaaacgag gctcttaaaa aattattttc cgatgtcgca ccttcctttg aaaaaagacc
     4021 gggaggatac accagaatag taaagctagg ctatagagat ggcgataatg ctcctatggc
     4081 aaggctggag tttgtaaaaa caaaggaaac caaaacaaaa gattctaagg caaaggttac
     4141 gccagaaaaa gccgacggaa aaaaaacaat agaaaagaaa acaaagggat ctaaggtatc
     4201 tgagaaaagt gaatcagagg tcgtggttga aaatgctatg gaagtaatag atgatagttc
     4261 aacaaatgta gcgaaagaac aataattaaa atgaaaatac ttaaaacgac aacaccaaaa
     4321 ataacatata tacaacaaga gtggcatttt atagatgttc gcggaagaat attaggagat
     4381 gctgcgcaag agattgcaaa attactcatt ggtaaaaaca aagccattta tacacctaat
     4441 atcaatgttg gggacaaagt tgttgttctt aattccaaaa aagtagctgt aaccggcaga
     4501 aagcttgacg acaaaacata ttacagacac actggttatc ctggaggtat cagaaatgaa
     4561 aaccttagga gtttattgga cagaaggcct aacgaagtca taagaagagc agttaaaaat
     4621 atgttaccca agaacaaatt acaaaaaata agaatggcaa atttatatat atatgagggc
     4681 gatcagcatc ctcatgaggc gcagcaaaaa tgaaaaaaca gcaaacagaa gttcataaag
     4741 aaataatgaa gccgcgaaaa gattttatat cgggaaccgg cagaagaaag acctcggttg
     4801 caagcgtttt tctgtataaa gaaaaaggcc aaatactgat aaatggcatg cctattgctg
     4861 agtactttcc ttccgaaaaa gaaacattag cttggactaa accttttcat gcaattggtg
     4921 tttctcaccc cgaagcacag tttagcgcaa ccataaaagt atctggctcc ggaaaatcag
     4981 cccagttagg agctgtagtt ctcggaattt ccagggcttt agctaaaata gacgaagaat
     5041 tcgaactgaa tttaaagaaa ttagatctat tgactcgaga ttccaggatg gttgaaagaa
     5101 agaagcctta ccttagaaaa gctcgaaagc gacctcaatt ctcaaagaga taggagctta
     5161 atttcagcgt atctctattg tccgctttac ccatacctcg cagttattga gattaaaact
     5221 gtctaagctc ggtttttaat gtttgtaacg cttgctctaa aacttcgagc tgtttttcaa
     5281 taaattttat ttttgctatt ggcgggttca caaggacagc attgtatgtg tgtgaaattt
     5341 gctcaagtac gtgatagggc ggagaaattc gaccgcactc ataggccgaa atagttttag
     5401 ccgataagcc aattttttcc ccaaaccttc tttgagaaag tttattgtct actctaactt
     5461 tttttatttt tttaccaatt ttgtagctga gcatatctac taaagttaga atacaatata
     5521 caaatgcatg ttgcaataac ctatattgtg agtatgcata aaaattccaa actattcgat
     5581 cgaacagatc ttttgccacg ggagaaaatt gaaactctcg gctcctcaca tcttaccgac
     5641 gcagagttgt tagcaataat acttagaaca ggtgttagcg gagttggtgt tattgaacta
     5701 agtactaaca tactgtctca gctcggagga cttaggtgtg ttattaatgc tccaaaagaa
     5761 caactcttga aggtgaaagg tttagggcct gccaaaatat cctcgctcct cgccttaaaa
     5821 gaaatgtttg cccgcctaac gactgttgaa aatatacgaa atagaaaaat atcaagccct
     5881 aaagatgtat ttgaggtggt gcgttctaga cttatgaatc aagaggtgga acatttgtat
     5941 ttactaaatt tggacaccag aaataactta atttcgttag atctgctgac tataggaaca
     6001 gtaaatcaaa ctttagtcag ttgtagggag atcttccgaa tggctttgct taaaggtgct
     6061 gtttcgatca ttttggttca caatcatccc tcgaatgacc ccacacccag ttccgaggat
     6121 atttctctca caaaaatttt agcagaattt ggaaaaggct tgggcattaa tttaatagac
     6181 catgtagttg tttccgactc tgaatttata agcataaaaa gcatgtttca gctagattct
     6241 gcaaaggggc aaagagatga tgaggaaaat tttggcaata aatcattgca taataaccaa
     6301 gaaataaggt cgcaagatcg agttcatata cctataaatt cttataataa aagtttttca
     6361 cataaatttg tttataagca agcgctactg tagagagtat ttagaaagga ggtgaaaatg
     6421 tttaaaaaaa taaataaaaa aatgcatgca agtgcgggtg tgttggttct cgtgttattg
     6481 ctttgctctt ttgcgggtgt tgcttatgct gcttttgtcg acaaagggca agtgcttggc
     6541 tctactttta gtgtaggcag tgctgatcta aagctgttga gggacctgtc tttaggtgcg
     6601 gatggcgcaa atttggcaga tgagatacaa gggccgtctt ttagtaatat ttcgtctaac
     6661 tggactgcgg actatttagt gaagttgtat aacaacggca gttccagagt cacagtaact
     6721 actaattccg actatctcac agccaatgat cccgatgagc ttaggcagct aatatacgtt
     6781 gagataatag agtggaatga cgctaactca gatgggacag ctgatgagac tgagctcgga
     6841 gtatcttatg gtagagatac gattgtaaag tggaagacac agggatttag tttaggaagt
     6901 attgacgcgg gggcagtaaa gggactggtt gtaaggtttt ctaccggtac cgtgtccgat
     6961 acaaagcagg gggctgtcgg tatttttgac tttgagttta attcgattgg ggagtaaaga
     7021 atcctgaggc cgggtctttt gagatccggt cgggcctcgg gcgcatgccc gaggccccac
     7081 tccaacccaa aaaccacata tgctatcatt tatttatgtt attcatcctg tctcaaagac
     7141 taaaactttt tttgaaatgg tattttcttg attcaactaa atacttattt gtggtgggtg
     7201 ggcgagctct taaaatattg aataacgatt tttcgttatt attaaaccta cgacttcttt
     7261 tcactccgct atacggcgat tacacaattc aagggcgaat ttttggtttt atgatcaggc
     7321 ctttagaagt tattcttgga tttttagcct tgaccctatt tctttgtctg tatcttggta
     7381 ctatagtgct ttggctctgc gcaccagttt ttttgttttg gatttatagg tgggaggcaa
     7441 ttattccctc tatcttactt gtgcttctgt atctttttaa atatagaaaa acccctgtat
     7501 caagagtagg cgccgtagat caggataagc tagcttacac ctttcaactt gatgctttgg
     7561 acttatgttc cgaaatcctt aagggggcga gttggaaaga ctcgttgaaa tcttacagaa
     7621 cccactattt gatgaaaaaa cttgaaatag attatgagtc atttttactt ctcttgaatc
     7681 aatatgaact ccaagaaaca gacctcataa aatcttctta tgatattgcg agccggatga
     7741 aaacaagata tgttgagttt gagcaagcat tctatgccat tttaggcatg gttgtgaaaa
     7801 aagagaagtt tttatctgag ttcaatcttc gtgatgagca ggtgttggag tgtgtatctt
     7861 ggattgtagg tgaaagagaa aaactttcag cagcacattt ctggcagctc gattatcgaa
     7921 tgaagagttt gggtggggtt ggaaaaggca tgacgtaccg tgttacgcct tttcttgatt
     7981 ctatttcctc cgattttacc aacatggcca gtaaaggtta ttttgacgat attattacgc
     8041 gcgaggatat agttaatgaa atagctagtg ttcttgacgg ctcgcgagac aatgttttac
     8101 ttgttggtcc agccggatca gggaaaacct caatcataaa aagtttgtct ttagaagtta
     8161 tgcgaggtac agagtacaag tctttgagga ataataggat tgtttctctt gatgtcggat
     8221 cattaatcgc cggtacaagc tcggcaggcg aagttgctca gagaatagat agtgttatgg
     8281 ctgaggtaaa gggatctcgg gaggtaatac tttttattga cgagattcat aatatagttt
     8341 ccggagttgg tggtgacgcc ggtagtagcg tgttagcatt tttagagccg cacatttcaa
     8401 caaaaacagt acgaataatc ggagccactg agaccttgag ttatcgaaaa tacattgaac
     8461 cgaacgcatc ttttgagcgt cagtttgaag taatcagaat ggaagaggct agtgaagcta
     8521 cagccctgga gattctaaag tctcaggcaa gaaaatttga gcttgggcgc ggggtaacca
     8581 ttacatttcc tgctttgctt gaggctatat ctttatctaa acaatatata ggcggcaggg
     8641 ttttgcctga ttctgccatc aatgttctag agagaggggt cgttagcagt cttagtacag
     8701 atagggtcgt gaatagagag gtgatcaaga aagaaatttc acgagtttct aatgttcccg
     8761 tgactacagt ttcggatgac gaaagcgaca agctgctaaa tttagaagaa gttctcaaaa
     8821 aaagggttat tgggcaagat caagcaattt ctatgattgt aaaagctctg cagagagctc
     8881 gtgttggaat acgaaacaag aacaagccta ttgctagctt tttgtttgta ggtacaacgg
     8941 gcgtaggaaa aactgaaaca gcaaaagctc tagctacgga gtattttggg gatgaagaga
     9001 gaatgattag attggacatg agcgaatacc aggaggtaag tagtttggat aaactcatag
     9061 gaacttctga tggaacaaaa cgtggcttgc tcacagacgc agtaaagaac actcccttca
     9121 cgcttgtgct tcttgatgaa atcgaaaaag ccgaatcttc ggtattatta acctttcttc
     9181 aggttttaga cgatgggaga ctcacagatt ctatgggcga cacagtaaat tttacaaaca
     9241 caatcataat agcaaccagc aactcgggca ccaaaaagat acaagacttg gttgctcaac
     9301 gtgcttcttt tgatgagatt caggaagcag gattaggcgc tgttcgagat aaattcgccc
     9361 cagaatttct taacaggttt agtgggataa ttgtatataa acctttagat agggaatctt
     9421 tgagaaaaat agccctttta agtctaaaaa gtgtagagcg tttggctcaa aataaaggta
     9481 ttaaagttca atatgaagat gggctgatcg acgctcttgt tgacagaggt acaagcattg
     9541 agtggggcgc tcgacctctc aacaggctta ttgaggatac cgtagagact tatttagcca
     9601 aaaagatact atccaaggag ttatctcaag gcgacgaact cgttttaaac ttggaggtat
     9661 taaataccta acgctaacta ataaaacacc tgatataatt cagattatgg aagagcataa
     9721 aattcaattg gataaattaa aagaaagcct gttttatgat gataagaagt ctaagatagc
     9781 agaattagag ttgcgacttc aagatgaaac tttgtgggca gattgggaag ccggacaaaa
     9841 agtctctcaa gatctggctg agttaaaaag agacgttgag gatatcgagc tcttggaatt
     9901 gtacctagaa gaggaagatt atatatcttt tgaaaatgag gtgaaaaggg tcgaaattaa
     9961 aacctactta tctggcaaac acgacaagga taacgctatt ttgtcaattc atgccgggca
    10021 aggtgggacc gaagccatgg attggacaga aatgctctat agaatgtaca caagattttc
    10081 tgagcgaaag ggttggaaga cttccctttt aagtttggtt aagggcgaag aggcgggcta
    10141 taaaagcgtt cagcttgagc ttagcggtaa gtacgcatat ggcctattaa aacgagagtc
    10201 tggggtacac aggcttgtca ggcaatctcc ttttaatgcc gatagtctta ggcaaacatc
    10261 ttttgcgtta gttgaagtta taccctatat agaagagggg gaagaggttg aattgtcaga
    10321 atctgatata acctttgaat cttttaggtc cggcggttcc ggaggtcaga atgtgaataa
    10381 ggtttctaca gcggtgcggc tgaggcatgt gcccactggc atagtggttg aatgtcagga
    10441 agagcgtact caagcgaaga atcgggaaaa agcaatgaaa gtattaagat cgaaagtata
    10501 tgccatagaa ctacagcgag ttgaagatga gaaagctaaa cttaagggta actataaaac
    10561 cccgggctgg ggtaatcaga tacgaaatta tgttctgcat ccttataagt tagtcaaaga
    10621 tgttcgaacc ggcattgaat ctacaaaccc cgacgcggta ttagacggag atttggacct
    10681 ttttatacaa gaagagctag ggctttgagc tcaattgtat aataatttgc ggcagacttt
    10741 gaaaataact agacaaaaaa tttactcaat actataattc cattatggag atcgataata
    10801 cacagttata ttcctcagga ctagttcaac ctcaacctaa gaagaagata gacaaaaaga
    10861 atttgactat ttttataata gcttttgcca tacctgtaat tttaggtctt ataggttatt
    10921 acgtttactc tgtaaaagct cgtaatccga ttgatttaaa aagtcctctt tcgagatctc
    10981 aaaatgacgg ttctcagaag gccgatgtca tgaatcctct aaccggtgtg ttatatactc
    11041 cttttgctgc acaggaatgg ttggacaata ggcccctggc agtaatggta aacaatcatg
    11101 ttgatgcccg accgcagtca ggcttaattt atactgatgt tgtatatgaa atagtcgctg
    11161 aaggcggtat aacaagattc attgcatttt ttttaactaa tactcctgaa aagatcgggc
    11221 ccgtgagaag cgcgcgacac tactttttta taccggttaa agaattaggg gatgcgatga
    11281 taatgcatat aggatggtct ccccaggcaa ttgaagctat agatacctgg ccggtacgaa
    11341 gtctttttaa aggcggggct gacttttggc gggataatcc cagaaatgtt gccaccgaac
    11401 ataccgcttt tgttaatggg aagtatctcc gggaagtggg caatgaatta ggttgggaag
    11461 ggaaaagaga aattacccct tggaaattta aggatgaaac aattccccca gttgctaacg
    11521 aaataggttt ggttggggaa tttgaaccca ttcaggtaaa tttttggtac gagggcgact
    11581 atacggctat ttggaaatat aatcgtgaca caaattcata tcttaggttt tcaggatttg
    11641 acctagaagg caatcctatt cctttgtttg atcaggaaca gacaacaacc caagttagtg
    11701 caaaaaattt aattgtgctg tatgtaacag aactttctat agatggcgac gataagaaca
    11761 gattagacta tgagcttgta ggtagtgggc agggagttgt atttattgac ggtaccagca
    11821 ttcctgttac ttggagcaag gcttctagag atgatcgaac tatgctgtac gatgagagtg
    11881 gcaacgaagt tgtgtttaac aggggaaatt tttggatcat ggtagttcct gatagaaata
    11941 tagaccaagt aatttattaa agtcgataag tatttagttt aaatactgcc ccctaactta
    12001 tatgttaaaa gatttatttg tatctgaagt aagagtaaaa atactagcat taatgctgcc
    12061 aaatcctgca gaatcgttgc atgtgcgagc tatagtaagg gcaatagaca ccgaaattaa
    12121 tgctgttcgt cgagagctgt caaacttata caacataggg cttatgaaac gtagacaaag
    12181 cagtaacaga atttattaca ccgtagacac ctcacacatt ttatacgcag aacttctctc
    12241 tctagtttca aaagaatacg gcttgggggc tgacattata gcatcaagga aaaagctggg
    12301 agatattaga tttgcaatat tggcgaggag attttctcga aatgagccct caaccatgct
    12361 ggatatagat ttattcatag ttgggagtat aaatcttgaa gttttggatg ctattgtaaa
    12421 gaactatgag cagagtctag gtaaagaaat tcattactcg gttatggggg cagaagagtt
    12481 tttatttaga aaaagacgta acgatcaatt catctcaaaa gtattaagcc aaggccggac
    12541 aatgcttgtg ggagatgaag aagaattgtg ctcgatataa aatgaaaaga tggcaaattg
    12601 cgctagagca aagatccgat gacataataa aagatcttct taaaattcgt gatatcggag
    12661 aagacctagt agaaagcttt ttgaacccac ctaaattaca agaatatatc agaaatttcg
    12721 ataggaattt tattgaatct ctgagaaacg ctaaaaaagc tatactagaa gcagcagatg
    12781 caaatgttcc tataataatt catggagatt atgacgcgga tggtgtttgt gcgactgctt
    12841 tgatgcataa cgttcttaaa aaagaactcg gctattctag tgtttataca tttatcccaa
    12901 atcgctttga gcacggttac gggctctcag aaaattctgt agccgctgtt ctcgggatgt
    12961 taataaaaaa cggatattct cctgatacaa aatttgtgct catcactgtt gatagcggta
    13021 taacggcctt taatgaaact gcacaactaa agaaggccgg ccatacggta ataattactg
    13081 atcaccacca aaaaccacaa aatcttcctg aggccgatgt aatagtgtgg aatgatcaac
    13141 tggttggtgc ttctatagct tgggtgttgg gccttgttct tgggtctaaa aatacatcca
    13201 atctcgcttt agcaagcatc gcaactgtta ctgacctcca gcccctaata ggttttaata
    13261 ggaggcttgt gctagatggg cttcacgtaa taagtgcaaa ttgcccactc ggcataaaga
    13321 aattacttga agtatcaggt agagatagta aagacattga tacttacgca ttaggctggg
    13381 tacttgggcc taggctcaat gcgggagggc gcttgaatga tgccgcccag gctttagagc
    13441 ttctcaccac tgatgatagt ttggcggcag gtgaaattgc cggcatgcta gctgatgtga
    13501 ataaagaaag acaagatatg acttttgaaa tgtatgatct cgccagtcta aatttcgatg
    13561 aagccagcat gcctaaaatc attgtctcag acgatgaacg ttaccatgag ggtatcatag
    13621 ggcttgtagc ttccaagttg gtgcaaaagt accacaggcc gtcaattgtt ataagtacag
    13681 atggggacgt agctaagggc tcagtaaggt ccgtaaaggg tataaatatt atagaaatct
    13741 taagaaactt tgaggacttg tttctttcgc tcggtggcca ccctatggct gcgggcttta
    13801 gtattaaaac tgaaaacatc agtcttttaa ggcaaaagct tgcagaatat acggaagagc
    13861 atattctaga cagccagctg gtaccttcat tagagattga tctagaatta atgcccgaag
    13921 agataagttt aagtacaaac tctctgcttc aaagactaaa gcccttcggt ataggaaatc
    13981 ctgagccgat ttttttaacc aaaaatttag ttgttgtatc aattaattct tttgggagag
    14041 agcaagcaca cattagtttg cagcttgcta ctgatgatat aaagattaaa gcgaagatgt
    14101 ttaacggtgt cgagcacgac ataatgaaac ggcttgctct gggtgataag attgatattg
    14161 tgtttagaat gtcggaaaat ttttacaacg gcagcactta cctagaagtt gtaatagtag
    14221 attttagata tagtgtttga aaagcatgtc tgataaaaaa gtaataaaaa actcaaaagt
    14281 gttacttgcc ataaaaaaat tactgtcgga aagcggagtt gagtataaaa tgtttgagca
    14341 tgagcctgta tggacctccg aagaagcttc aaagataaga ggaacccctt ttgattccgg
    14401 agctaaagca ttagtattat atgtggatga tgttccctcg ctgtttgttc ttcccggaag
    14461 tttaaaagca gattttaata agataaaaaa attgtttatg gcagaaaatg ttcggttggc
    14521 tacgcccgtt gaggtagaag atcttaccgg cctagttata ggcagcatac ctccgtttgg
    14581 aagtgtgatg gggcttcata gttattttga ttctaaactg ctggagcacg aagttgtagc
    14641 atttaacatc ggagctcata cagcatcggt tattatgaag cccgagtcac taatggtcat
    14701 agaaaagcct aaaattttgg attttagcat atagcaatgt ttgagcagct cattataatt
    14761 ccaatactta tagtgctttc tacattgaaa tttcttgatt ataactattc ttcgaatact
    14821 acctataaat tctctcgatt tggattttta cttcttgcaa ttttttttgc agttttcgtt
    14881 atcattccac taggcggctc cataggtcgt atcacggatt acttattagt tactaattat
    14941 aactctgcct acaattactc ttttggcgac aaacaactct ttcaatctag tttgccaaaa
    15001 agaataaata atgcggcagt gcctgaaatc agtgctttgg cgtacataat tgtggataag
    15061 aacacccaaa ccattctttc cgaaaaaaat tcaatgcaga agcttcctcc tgcatccact
    15121 accaagatga tgacggcttt aactgcatat gatttatatg atcttgatag tatgttgctt
    15181 gtgcctgcta cctgtactcg cggagataat cagaaggccg gttttcaggc cggtgattat
    15241 gtttcagtcc gcgatctatt ttttagttta ttggtaagct cttcgggaga cgcagcatgt
    15301 gcattggcta gcggttttgt aaatactttg ggaggagagc agttaataac ctacgataaa
    15361 tttattgatt taatgaatct taaagctgcc tctttaaacc tagacgatac agttttctct
    15421 aatcctattg gtttagatga cgccaatgga aatcacgttt cttcagcttg ggatttatat
    15481 ctcctttctg ttgaactgac taaaaacact ttatttaaac agatagttca aacaaaagaa
    15541 tacgttttat acctttccaa caagaaggta ataatgacta ataccaacaa gctccttttt
    15601 gacgtggaag gtacggttgg catcaaaaca ggaacgacgg ccggggcggg agaggttttg
    15661 gtgtatgaat ataataaaga tagcaccaac attgtgattg tcgttatggg aagttcagat
    15721 cgttttactg acacaaagaa tcttttagaa tgggttatat ctagttacaa attttaacta
    15781 aactcacaat tagtcactaa gttcgatata gttcgaagca acgatgcttg agggttgtca
    15841 gtattactgt acgggtgagc ttggctgtgt tattggagtc ggggtggtgg gaattgcttc
    15901 caacggtttt atatattgtc cttgtattgc cgggtaataa atagttattt ctccgcccgg
    15961 cgcgtttcca atgttatatt ttccgtcaaa aacatatatc ggttgaagat attgctgttt
    16021 ttcgggcgtt tcgtagtaag ccatgtatat attttctatt aatgctctat ctactcttgg
    16081 tgccgtagta gaatcaaaaa cggacgatcc ttttggcgtt acatttacta ctactccttt
    16141 acccgacacc acggcagccc aagcctcgtc tataggaatt attggatagg tcgcttctgt
    16201 gtttgggatg atttcgtgca catctgctgt catgaaaggg tagttcattt ctgtgttttg
    16261 gatttttgca gcacttaacg taacgtttat tagaccgatt ttaggatttg gtcctaggat
    16321 aggtactgtt tcaatttgtc taaaaaagtc aaccctagca aattggatct ctgtattatt
    16381 tgttgccata gttaggttgt ttccttgtat tttcccgaat gttactgtgt tcgtgccttt
    16441 tgaatataaa gggtccgaga atctattaat ctgggtaagg atggatattg ctccttgagc
    16501 cgctgaaccg gagcttatag tctgggcttt gtacataata ttttttccaa ctaatggagt
    16561 ttttaactca agcgactttg tgtttttgtt tatattaagt gttcctccgg atattttgct
    16621 tctccaagaa tacaaatccc ccttaagatc tgttatcagc tcgtcgtctg tgaaacctag
    16681 cttctgagca ttgtcttgag cagctcgccc tgcttcatat gagaaataat ccggcgcaaa
    16741 ttcgtatacc ttcattctgg tcggcatgcc ggccggaagc cttccattgc tggtatttag
    16801 ttctatagag aaattattat ttgctaccgg tttctcaaca aactcaagag gatcgagcat
    16861 tccaaaaatt ggattcggta gatcttttgg cgggaagaca taataaaaag cactaataac
    16921 tgcaggtttt gtaataagga atagaatgta tagtacaagc agtatcgcta gtatgactgt
    16981 gagccttttt ccaattacag aagccttcac tagattcatt ataataatat tatcacggta
    17041 tgctttatgt tagaatggtt ctatgaaaat gcgtaccaga atagcaccaa gtcctactgg
    17101 ggagtttcag ttaggtaatt ttcgaacagc cttatataac tatgctttgg caaaaaacac
    17161 ctcaggagct tttgtcgtta gggttgaaga cactgacaga aatagatatg tagagggttc
    17221 tatggagcga acccttgagg ttttagagaa gtacggaata cccggagatg agagccccct
    17281 ggtcggaggt ccttacgaac cttacattca atctcaaaga ttagagttgt acaaggaata
    17341 cgcaaagaag cttcttgata gcggacatgc ttactattgt ttttgctcaa aagaacgttt
    17401 agaagaatta agagatggtc agaggaagtc aggtatggcg gctaccagat atgataaaaa
    17461 atgtttatct ctaagcagtg aagaagttca aaaaaagcta gatcagggag agccctatgt
    17521 tattaggctc aacgttccaa aagatgaact tattaaagtc gatgatttag tttatggcga
    17581 aattactttt gattctaatc aaatagacga tcaggtgttg ataaaaggag acggttttcc
    17641 tacttaccac tttgcggtag tggtcgatga ttatattatg aacataaccc atattcttag
    17701 aggtaatgat tggattccct caaccccgaa acatatttta ctgtatagat tttttggctg
    17761 ggaagacagg actccaaaat tcattcacct gcctttgctg aaagagttgg gatcttcgaa
    17821 aaagctcagc aagaggttcg gtgctgttgc tgcaatggat tttctaaagc agggatattt
    17881 gagagaagca gtattgaatt ttgtaatgtt tttgggctgg aaccccggaa ccgaaaaaga
    17941 gatatattcc ctcgatgagt ttattaaaga ttttacaata gagaaaatag gaaagaccga
    18001 cttagttgta atggatagag aaaagctttt gtggtacaac ggccattata ttcgctcctt
    18061 gtccgtcgag gatctcatgc tcagacttgc tgcttgggcg caagagcatg agattgaact
    18121 gccccaagcc gataaaaaat atcatctaga agtacttagc ttggtgcacg aacgaatgaa
    18181 aactttgtca gagtttgtgc cgcttacgca ctatttctat gcggatccaa aagtagaccc
    18241 ttccttaatt gtttcttttg gaggagacga atcagcaagg ataattaaag atctattatc
    18301 tttatacaag gccgttgacc caaaaaaatg gacctccgcc gatttagatg aattgtctca
    18361 ctcatatgtg acgaatggcg gttacaaaac aaagcaagtt tttatgtctc ttcgggccgc
    18421 agtgacctcg gaaactgcaa ctcccccgat tttcgataca ttagaacttt tgggtaaaga
    18481 gacaacctta aggaggttag attctgcttt ggcccggctc tagttttgat agattttaac
    18541 gtacaattaa agttcaataa gtatacaatt agtttaatta aattaaagga tagttatgcc
    18601 aaaacaaaat acacaaacag cccaatcaac tgcagataaa aataaagatt ctgctctaca
    18661 atcagctctt gctcagatag aaaaatctta cgggaagggt tcaattatga agctaggtga
    18721 aaatagtcct caaagaaata tacaggttat acctacaggt tcaatcgcgt tagatatagc
    18781 cttaggtctc gggggcctgc ctaaaggacg aattgtggaa atctatggtc cggagtcctc
    18841 aggtaaaacc accttagtgc agcatataat cgccgaggtt caaaaggcgg gaggcacagc
    18901 agctcttata gatgctgaac atgccttcga ccctgtctat gcagagagaa caggtgtaga
    18961 tgtaaataat cttcttgttt ctcagcctga taccggggag caggcccttg aaatagctga
    19021 aaccttgatc cgctctaatg ctgttgatgt tattgctata gattcggtcg cagctttaac
    19081 tccaagagca gagatagagg gtgagatggg agatagtttt gttgcacttc aggcccgttt
    19141 gatgagccaa gctttgcgta aaattacatc ggccgtaagt aagtctaatt gtttggttat
    19201 ctttactaac cagctgagaa tgaaaatagg cattatgttt ggtaaccccg aagttactcc
    19261 aggaggaaag gcgctaaagt tctattcttc tgtaagactt gagataagaa gagtacaggc
    19321 attgaaatcg ggaactgaag atataggtat caaagcaaag gttaaggttg ttaaaaataa
    19381 agttgctccc ccttttagaa ctgcagaatt cgacattatg tttaatgagg gcataagcaa
    19441 agaaggtaat cttgttgatg ttggagtcga gatggatatt gtaaaaaaaa gcgggtcttg
    19501 gtatgagtac gcgggtgaaa aattaggtca aggccgagaa gccgctaaac aatttttgaa
    19561 ggaaaataca aaggtgagag acgaaatcga aaaatctatt cgagcaaaaa tgaaagactc
    19621 taaacctctt cctttagagc ttgtttccgg cgaagaatca gaagaaaatg gaatcgagga
    19681 gtagatctct tgaagctaaa attttcaata aggccgtagc ttatttagcg ctacggcctc
    19741 acagctctta tgagcttgaa aaaaaactta gtgcttattg ggtcaaaata tccgatgatg
    19801 tcgagtttga taaaagtact gttgttataa agcaggcgat tgatcggctt aaaaatttaa
    19861 actacttaaa cgacagcata tactttaaag atctagtgaa ttcggtcgcc aattccccta
    19921 accctaagag tcctcttgag cttaagcgtt atttatacga taggggtgtt tctcttgaag
    19981 taattgaatc tatattaagc gatctttata cctcgggtct gcaaaaaaaa gccatttcaa
    20041 ctctgatctc aaagcttagc ggtcgttatt cggagaagca gaagctggtg aattatctaa
    20101 ccggcaaggg gtacaattac gccctaatag ctgatttaat tgacaggtaa ggtattctga
    20161 actagaatca tacatgggaa agtttacaaa agtagagtaa acagacctat agcataagag
    20221 tttatctatg acgggcgcta agcctaaatt tataatctag tttaatgcca tgaatataat
    20281 atcagaatta ttaactaaat ctaataaagc ctttacctat cgattattac atgtatcgac
    20341 agcgccagct ttgcgcagac tggcttaatt tttagagcct ttatctattt ctgatatatt
    20401 gcattaactc gtttatttag gaaccgtcac agtgttttaa ggaggcataa tgcaaagtat
    20461 tatattaatt ctagctgttt tatcgtcttt tggccttggc ataaaggctt atcagtatta
    20521 ctcggtaaaa caaaaaaagc agggtatcga tgattccgca gagtcaaaaa aattagagcc
    20581 tggtttagat aaccaggatt cttctcagga ttccgacaaa ccaagtttgg tgtcggaagc
    20641 tcgtgcaaag gcacgagaaa taattcttga ggctaaagat tcctcacttc aaataaaaaa
    20701 ggaagcccaa gattatgcaa tgaaggtaaa gcaagccttg gagttaaagg agcgcgaact
    20761 cgttaaaaaa gaggctgcag tagaagttaa ggaaaaagag attgagaata ggcaaaatga
    20821 gctaaagagc gacagagaag aggttcagaa gaagctagat aatttagcct ctttagagtc
    20881 tgaactgcaa agcaaattag ctaagatagc ttctttaact agggaagagg caagggagca
    20941 gctattaaaa gtattcgata aagaattggc tgtagaaaaa agcaaaagaa tccgtgaagt
    21001 agaggaagag attaagcgcg actctgttga gaaggcgcaa gaaattttag tagatgccat
    21061 gagatttggt gcaacagatt atgttgtagc tcacactact tctaaagtta aacttcctga
    21121 cgaagatatt aaaggaagga taattggtaa agaaggaaga aacataagaa cttttgaaga
    21181 atacaccgga gtcgagttgg atttggattc ttcaccgggt gaaattatcg tatcgtcctt
    21241 tgatcctatt agaagagaga ttgctaaggt atctcttgaa aggcttattg ccgatggaag
    21301 aattcagccc tctcgcatag aagagattgt agagaaaacc accaaagaga tcgagcatat
    21361 tatgtataaa gaaggcgaca atctctgcca tagagttggc gtctacaata taccaaaaga
    21421 tttggtacag atgttaggta gattcaagta cagattttca tatggacaga acatgattga
    21481 gcacactctt gaggaaacaa gaataggtgt cgctattgct aatgagttaa aggtagatgt
    21541 tgaggttgta aagctaggat gtttgtttca cgacataggg aaagttgtaa atgatgaaga
    21601 aggtacgcac gtacagctag gagttgatct tctcaagaag cacaaaattg acgagagagt
    21661 tatcaattgt gttgctgagc accacgaaga cagaccgttc agttcaatcg agtcggcaat
    21721 tgtagctttg gcagaccatg ttagcggtgc gaggcccggc gccagatcag aggactacga
    21781 ggcttatgta caacgaatga aggctctgga agaagcagca atgtcttttg atggggtaga
    21841 gaagtccttt gctatttcag caggaagaga gcttagaata tttgttaccc cgtccaaagt
    21901 agacgacgat tctacagctt tgttggcacg tgagattgca aaaaagattg agttagaaca
    21961 atcttacccc ggcgtagtta aagttaccgt aattcgagaa actcgtttta cagaaacagc
    22021 caagtagaga ttggtttatt ttatgttatt ggccttaggg ggcgcaagca tagtgaaaaa
    22081 cggtgcttgc gccggaaagg cagaaagtaa gtaatttaaa aagataatat gtattacgtt
    22141 tatttaataa tattaggttt tatcgttttt gtttttatag caaaaatttt gaaatctatc
    22201 ataagaggat gcataactgc aacgttattt gttttattta ttgcaattgt gttgtattat
    22261 ttaggttata gagccgattt tttatctagc ctctacaact tactgccgtg attgggattc
    22321 atgctcactt atttttgtag catgcaaaat tgaaaaatat gagaatttta tttataggcg
    22381 atattgtagc caagcctggc agagaaatag taaacaacat tctgcccgag attaaagtaa
    22441 aagaaagcat agatcttgtt attgcaaatg ttgagaatct ctctggcggt aagggtgtaa
    22501 ctagaagtac tcttaaagag atgcaggatg caggagtaga tttttttacc gggggcgatc
    22561 atattttctg gcaaaaagaa gtcgaagatt gttttcagga atttccatta ataaggccgg
    22621 caaattaccc aagcacatgc tcaggagaag gctacaaggt tttagataca ggtaggaacg
    22681 ggtcggtttt aattgcaaat ctcattggta gaacctcttt tgggggaaat caggcatata
    22741 cagatgatcc ttttacttta gccgataaaa tactttcaga tacagaaaac ttagatatag
    22801 cattttcatt aattgatttt cacgccgagg caacaagtga aaagcaagca ttcgcttatt
    22861 atttagatgg tcgcgttggc gctgtttgcg gtactcacac ccatgttccg acttgtgatg
    22921 aaagagtttt gccttctggt acgatgtatg taacagatgc gggaatgact ggaaacattg
    22981 attccgttct tggtgtaaag aaagagatta ttattcagca atatcttact gcacaaaatc
    23041 agcgctttga gtgggaaaat actggaaaaa aagcttttag aagtgtaata atagatactg
    23101 atagcaaggt gattagtcga ttagatagaa tcagcgacta gcctctgtta attgagagca
    23161 caagtctctt agtaattagt tgataataac ttagaaattt gaaagggggt gtagcaagat
    23221 gtcaaaacaa gatatagtaa atgcattagc acaacaaggc atgaccaaag ttgaagcagc
    23281 tagagtttta gaagctttaa caacaatcat aaccaaagca cttgcaaaag gagaaaaagt
    23341 aactcttacc ggattcggta cattcgaaac aaggaagaga gctgcaagag caggtagaaa
    23401 cccacagact ggggttgtta ttcaaatacc agcaaccaga atagctgcat tcaaagccgg
    23461 taaagctttg agagatgctg ttagaaaata agcttagttt aatctaggtt ttttaaggcg
    23521 ggccatcagg cccgccttct cgtttctata agcaaaagcc gctctgaaga agaattaatt
    23581 gagtttagag ttttaaatta ccttatgcta aggctgctgg gtgctcaaag aaaccgcatc
    23641 ttcctgttgt ttggttgttg gtaaagtaaa ccaaaaagta ctgcctttgt cgggaccgcc
    23701 tgagaatacg cccacctcgc cgcccatttt agacacgtat agttttacaa tgtataggcc
    23761 cagtcctgtg cctccggcct cagcaaccgt cttgtaagag ttttctagtc gttggaattt
    23821 tttgaagagc atttgtgtgt ctgttgaaga aattcctttt ccggtatcaa ttatagaaaa
    23881 tttaacgtgc cttgcatttt taagtctctc tattttcaca gttattcctc cggtttgtgt
    23941 gaattttatg gcattaccga caaggtttac aaaaatctct ctaatttttt gcctgtctcc
    24001 ataaacatat ctcacgtttg ttttgttttc cagctttaaa taaagatctt tttcggcggt
    24061 tttaatttct aactcttcaa taatatcggg taaaattaga tctaggttaa gccttttttc
    24121 ttcgatctta actctgccct cttctatacg tgaaatgttt agcatgtcat taataagcgc
    24181 aatcattcta tctgtgcttt tcatcgattt ttctacataa atagcctgtt ttttagataa
    24241 tttccctcct tttcctgcag aaagcatcca caggtagctt tttattattg acattggtgt
    24301 tctaagctca tgtgaggcaa tggatacgaa gtcatccttt gttttttcca attccttctc
    24361 ttttgtgata tcactcattg taacaaggtg ctctatgttg ttttcgcctg ttttaataac
    24421 agacactttg atatgcacat agtatttttt cccttcgggc gataacagaa tggcggtatc
    24481 aggaatccca aaattttcag tcattctata aaatgatgtt atatcgatcg cctccccctt
    24541 ctctgttgtt attgaaatta tgtcagaagc ttttttccca attacttcag cagatgttgt
    24601 gtttagcatt ttctccagcg tgtggttgaa gagagtgatt ttgcctttag ggtctgttgc
    24661 gtagatcgcc tcggacactt tatatagtag aagctcggcc ctctttcgct cattgtagag
    24721 ttcgagattt ttcttatata tttcctgagc aaccttttgg aattgatccg ttttatttga
    24781 tctcattttt tagaccagga gagtttgagg tataacagtt cgaaaatcat taggaaagtt
    24841 ttcccgtaga atatttacac acacatctac ggaagcttgg ccgaatatct ctctgtagga
    24901 ctcaattaat ctagttacta cttcgttatc ggcaccggaa aaatgaatct gctctatagt
    24961 cagatttctc gcatctttag acgaaatctg aattaaacct gtgtttacgg cacgttcgag
    25021 agcaagattc tctcctatta tctgtgtctg tttcttaata atagctagaa ctagcctttt
    25081 cagctgctca tcggtcattc gcgttctcct ctagtagttg atataagttc agcattaatc
    25141 atcatgcccg ctaatgaaac tagggaagtt gctatgtaaa atagaaaatc cggcatgctg
    25201 gcgctgtatg cttgtccaag aggttcgagc aaagagtagg agatgtcccc aatatactgg
    25261 aatacaaatc ctgctaaaag gcagtataag cctactttca ttttacctcc cagacggctg
    25321 aacataatat ttattgcaac taaggatatt acgacaatgc tgccgaatga atatagaagg
    25381 tctaccgcta tagcaaggtc aaaagtttcg aaataagaaa ggtcgttgaa aagaagatag
    25441 agtgtagtga atgttaatgc cacaattgtg gttgttagaa gtgatacatt aaggactaag
    25501 ctctttttaa tgttagagac aattcctccg actaagatta ggttaaacat tgcaaaagag
    25561 tagaaaggat cgatgaatat aaagaagatg tctggcagcc cggggtaagg tacttcttca
    25621 acaaagaaag aaataattgt gtaagcgagc tgcccaagtg accaagcaaa cagccctaat
    25681 gatattaaaa ttaaaattct accgaccgca cttttgcgcc cgttccattt ttttgaattt
    25741 attgcagctt ttacgcttcc ataaagaggc tgtgcaaata gcactattgc aaatatgtca
    25801 taaaaatatc tgctttccgg actaaaaaat tgtagtgcta tccagtagat tatccaaaat
    25861 atagttaata tgatatagat tttaggcaga ggcagagatt tcattgatta attatcccat
    25921 ttttgttttt tattagtcaa gttatttgtg gtagcatagg gtgtgcattt
//
LOCUS       DAOSWN010000003        21056 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_1594_length_21056_cov_4.459883, whole genome shotgun sequence.
ACCESSION   DAOSWN010000003 DAOSWN010000000
VERSION     DAOSWN010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 21056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21056
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1594_length_21056_cov_4.459883"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..896
                     /gene="speB"
                     /locus_tag="PLF29_00455"
     CDS             42..896
                     /gene="speB"
                     /locus_tag="PLF29_00455"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HXK52448.1"
                     /translation="MNKKYAQYKGSKACIISIPYEHTVSFMAGCDLGPSAVLHASANV
                     ELYDEELQFKPHIYGISHVDVADFSEDHDANLKYIGKEFLKHYKSGKFIVSVGGEHSI
                     TIGLLEGLKQVEEDVSIIQFDAHADLRSFYPPSKKSHACVMRRASEMFPNTLALGIRS
                     MGEDAASYIKKNDYPILYAKDLFEMSDKEVQEYLSKVIRNKKVYITFDVDVFDASVVS
                     LTGTPEPGGLGWYQTLRILKHIFKKYDVIAVDVVELIGRESPTKCAFNTALLIRKMLG
                     YKFLSFQN"
     gene            1011..2030
                     /locus_tag="PLF29_00460"
     CDS             1011..2030
                     /locus_tag="PLF29_00460"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017943322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HXK52449.1"
                     /translation="MPYSKKDLLAKPIEHIDITKLEPTSFVDSMGSMGFTSREVSNAA
                     QIFVDMINDESCTVILTIAGSTSAAGCMKIYSDLIKYNLVDVVVATGASIVDMDFFEA
                     LGFRHYVREFEVPDDTLRDLYIDRIYDTYIDEEQLQVCDSVVRDIANSIGEGVLSSRE
                     FIVEMGRYLEKSGKKEDSLVLQAYKNQVPVFCPAFSDSSAGFGLVLHQAERPEKHVSI
                     DSVKDLRELTAVKIASENSGLMMIGGGAPKNFAQDTVICAETQGIEVPMHKYAVQITV
                     ADVRDGACSSSTLSEAQSWGKVSLSHQQMVFAEATSVLPLIASYAYHNTSWSSRKRKR
                     LSAIL"
     gene            complement(2118..2540)
                     /gene="speD"
                     /locus_tag="PLF29_00465"
     CDS             complement(2118..2540)
                     /gene="speD"
                     /locus_tag="PLF29_00465"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HXK52450.1"
                     /translation="MTAYKNIETTTFGPHLIFDAYGCPYKTLSNMELCFGILSDLADL
                     AGMKKMTEPYVIKATSNEFMGGKDPGGFSGFVIIQESHISIHTFAKRGFATVDLYSCK
                     NFEPESAVEYLKKTLQPRDVDVIKLDRGLKYPKQNIYK"
     gene            2597..2953
                     /locus_tag="PLF29_00470"
     CDS             2597..2953
                     /locus_tag="PLF29_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52451.1"
                     /translation="MDFLWPITFIASFSVISLIETEFYTLFGLKKVDIAEVVVGALAT
                     ALYFISLSLMRHSSFTVSILVLIIGWTVGNIVLSTIFLYVMGSINHDSKVFYEVIFWI
                     VLMIAAIVRMNLILGE"
     gene            2957..4003
                     /locus_tag="PLF29_00475"
     CDS             2957..4003
                     /locus_tag="PLF29_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HXK52452.1"
                     /translation="MGSKVSKSFKHHFLPHPSGKQRATLLTEAAIFTYCIVLVLSFLI
                     IRATPQLAPGVLGYASNINSEDLLRYTNQIRLQHGLGELTINQALNRAADKKAQDMFT
                     NNYWAHISPTNVKPWDFILSEQYDYAYAGENLAKNFDNSKAVVHAWYKSPSHRENLLS
                     PNYSEIGFAVRNGELEGYQTTLVVQMFGTLRNPSYSAQNQNQDFPGTSAVAEVPSSSS
                     GKSAENISQVNAQNELTLATLNQENIVAKNASESPLEPLVFKSSMSGTDGQEKVVLSL
                     DLSLTAKLLFGIFGSFILSLLFLDIWYSSKKGISRVSGHALAHIIFFNCCISRGFFCT
                     KFRHCPLIYHAYYR"
     gene            4260..5393
                     /locus_tag="PLF29_00480"
     CDS             4260..5393
                     /locus_tag="PLF29_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXK52453.1"
                     /translation="MKIVIVVPTYNEVENVGNLIPAIWQETKELSHDVRVLFVDGNSP
                     DGTAEKVKEMSALYPFVSVILEPEKRGLGAAYIYAFKYAMSEMSADVVVEMDADFQHN
                     PADIPRLIAALDAGADYAIGSRFAKGGSIPSDWALYRKLLSIGGSYFSKIVLGIFNVN
                     DFTSGFRASRVRGFVDKIDLDSILSKGFAYKIDLLYKMHKLGAKITEVPIAFGLRDRG
                     DSKMEKDNFQDSLKTVLTLRYRQNESFFKFLGVGLIGLFTDSALFNILYAVSLHDLLH
                     MSSSVESLVSGFIAMFVTFTLNNFWSFSARKIGSKIELAAKLGFYYATSYLPIVFRSF
                     LIHFSEQTFGESFFVRNGAFFIGIFIGLVWNFTIYSKVIWKKK"
     gene            5412..6161
                     /locus_tag="PLF29_00485"
     CDS             5412..6161
                     /locus_tag="PLF29_00485"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXK52454.1"
                     /translation="MEYTIVIPVYNESDKIANTINQVITFMRTFSEKFEVIVVDDGSS
                     DNTVQILENLTSSYKELVIIKNKHKGKSAAVWSGMYQAKGDYIYMCDADLATPIEELK
                     KLASWITEHNYDIVIASREGAGAVRINEPLTRHLAGRIFNAWVQAFALPGIKDSQCGF
                     KLFRREPAKVIFSKLQVYGENAPEINRPQFGAFDVEVLYLAKKFKYKVKELPVIWKYV
                     KTTRLNFFENTFRMSTDVLKVRINDLLGRYK"
     gene            6241..7647
                     /locus_tag="PLF29_00490"
     CDS             6241..7647
                     /locus_tag="PLF29_00490"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HXK52455.1"
                     /translation="MKKVCVIGTGYVGLVGAAVFADWGNSVVGVDIDEKKIAKIEEGI
                     MPIYEPGLDEVVLRNIKTGKLSFTTSLAEGMKDADMILICVGTPMSDTGAADLSYVWA
                     VAKEIGQNLKKGDKYVVIITKSTVPVGTNERVKQIVREYAEEGVEFDVASYPEFLAEG
                     RALEDMKETDRTVVGTENERALTVLREFYGHLDAPMVECDLRTAEMIKYASNAILATK
                     ISFINEIGQLCERAGADVGLVAKGMGYDKRIGRHFLNVSIGYGGSCFPKDVAALYKTS
                     TEEAYDFKLLRSVMEVNDSQMHHFVRKIRKVFGINMEGKKIACLGLAFKADTDDIRDS
                     KAIKIVRELRGHGATVSVYDPQAMENSRRELGDKDITYAEDMYSAVKDVDAVCILTEW
                     GEFKNIDIEKLWRAMGSSKRKFVFDGRNLLDQKKVEAQGFTYFAVGKRTNGYNEFLLE
                     DEEKRGFVTTALLKSQNG"
     gene            7659..8954
                     /locus_tag="PLF29_00495"
     CDS             7659..8954
                     /locus_tag="PLF29_00495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXK52456.1"
                     /translation="MSKDQKKKILYFVPQFPMLSQTFISREIGKLVEWDNLDISVLSM
                     SRGTGFASQKVLAKTHYQRLSVKICFEALIKYILLRPRKTWQAHRLISGEDSVPYLLQ
                     LKRKNFDPAADNSVLKRFHRARLVHFFKGVAFARMFEVYKPEHIHCHFLSDQSTIVMV
                     AAKLLGVPFSVSAHAKDVFVEGSLIPVKAREAKFIVVCNGNTWRKTIEFANLSASSGN
                     VHLLFHGFDYKDAYSKPLSVEKPAITSIFLGGTRFTKKKGIIYMVEASKILRDEGFAH
                     VVNLVGPADDLSTYNEIKDKIKEYGLEETVIIHGDGKGMPNEKVLEFYRISDIFVFPG
                     IETKEGDVDGVPTVLIEAALAKLPIISTDVGSVGDLIDSTNSIIIPQKDVGAIVDGVK
                     KLTADTALSKKLGEAVYIKAEKLFNIDINVRQLEQMLLD"
     gene            complement(9088..10020)
                     /locus_tag="PLF29_00500"
     CDS             complement(9088..10020)
                     /locus_tag="PLF29_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52457.1"
                     /translation="MALNNNEIRKKELEVYNLYLQKTGLSPATIKKLELSPEDVAKII
                     TKMTSADSTLRLFTNAKKETRDNYLKQSVTIREQGFIMVALTMLDAVQAWDIANKYAK
                     GIRETNDHKKIAYTLLADREKTNKGKLKYLTEASKCLEEALKATEGESISILKVHQAS
                     LNYDLALLHDTKSKEKKLKTALKMVDEALKTLPGSQAHRAWPLPIKAKILIELKLYDK
                     ALKVLQEAESCIYYGYEEEQNKNKQADMVLSVWTTGIALQMALLHIKTNKNTTARIYL
                     SAVINCPDAAGVLANRKLQAKNLLKKLEEPSKEN"
     gene            10144..11490
                     /locus_tag="PLF29_00505"
     CDS             10144..11490
                     /locus_tag="PLF29_00505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXK52458.1"
                     /translation="MHIAVFTKKTTFHKGYGGLETQNKLLVEELVRRGNSVIVFSPKA
                     EIETDGTVDSGVTYVFIPAVYRMGIFFQNIEDDVARFFEKFLPFINVSGLFSKKGKED
                     WKEKSLAYFSKYHAENRFDVVICQSSVGLGIINAKKQLGIKIVSISHGSILSEYVTRV
                     KELEIKSYFSPVALLSLFRDTAYVLVNYFTNQRRFIFGSDKIIAVSNSVKKSVIDETF
                     IPEDRVVVIHNGVEAGLFDKSLPAKNSSLYSKSANKDISSLAEYDGYVNLVFNGRIEK
                     TKGVFLLLPFVEELINDSEINVQLKMHVIGDGKDLAALKDKIQKYDLQDIFVLHGKLQ
                     YEQVVSLLKNMSIFIFPTLRKEGFPMVLVEAMMCGLPCIASDIGGNSDAVVSGETGFL
                     VSPGNISELVEACKALLLDKSLSAKFSDRALTKARSEFTLNNMVDGYLKVIEEVLQ"
     gene            11487..12728
                     /locus_tag="PLF29_00510"
     CDS             11487..12728
                     /locus_tag="PLF29_00510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXK52459.1"
                     /translation="MKILRIIYDWPPPSDGLAPHPYELSKAQIDLGHEVSVFSARWPR
                     QGPPADYSGKIKLIHFLGNSSLPFRTPAQGLMLVTVAPLLFFRYWFWRLKKSNSVDVI
                     HSHGHFALWIYLYRYFLRKFYKRAKELRTPLVVHFHNTVAGRFEAMNKSGKELKYLTE
                     KIDWPLARLSDETAVKIADACIFVSLDVFNEAKKHYNVDAKKCFILESGVNPDLFKPT
                     TIEEKDKTRKDLGLDPKDVVILHHGAMIDRKNTHCVVEALKYLPPHYKLLLVGPWPDS
                     KYRQTVDLIIEENRLSKRIVFVGYTPNEEVPIAYQASDIFVLPSSWEGLPKVAVQGLA
                     CGIPVLGSAFKIDDSVSGVFYLHDTKPETIARQIKEIIEGRPFVDVDLVSRIYSWRQR
                     ALQLNKVYDFVSTARSKNTRI"
     gene            12725..14539
                     /locus_tag="PLF29_00515"
     CDS             12725..14539
                     /locus_tag="PLF29_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52460.1"
                     /translation="MNMKKLPFIVLAFVILIRALFTNVNAAEWGDSYRILRAAEYLRN
                     YQYPSDEKRAPLFSLILAARPSKVDPVQWGRAEMLVITALLLTVFTYYVRYFISSPVY
                     QSLSIALLGFNPVFFYWSLRIMADVPFLLLVLCSFYLLKRQSTLGRFYWQKSFILGIL
                     GALGVLVRFEGYILICALFIGIIFLERGFYSWEETILQRKNLQKTPSAGKKRRTSKIP
                     ATVTNLKNIIRFVFSQLNLIFNLLRKKNREILAFVTAQLFILVPYWYYRNPLSSSYFE
                     EPSGRSYDLNTFLIYFASLFFISGFVPFFSYIFFRPAAGSLSSSTTFVEKDSKSSSTL
                     SFSAEARATIKKNLKDVISIGNENLGVSAFVIAELLLVLIWPAAIPRIFTPVLPFLII
                     LLTINLHNGLENFRSVMKDSKKMFLFFLSSQFFLLFLYVVLQFKYRLQFLLPIKEVFI
                     VMLVLQVLLVFLQLLKNKNVFLVSLAFVVFGWSLSTVFLHKDIFKVVRQANVYATSNL
                     KGTVGYNDVSSVSDWYLNQAGLNKSISGRFFDLTKKSNREYDVLLEQGFDYFLVTNEH
                     NTDLTIDLSSWPHLKVIKSFSSNINGKTFFTNIIEFEK"
     gene            14635..15420
                     /locus_tag="PLF29_00520"
     CDS             14635..15420
                     /locus_tag="PLF29_00520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXK52461.1"
                     /translation="MSRYREYPKISIITPSYNQGRFIKETIESVLSQNYPNLEYIVVD
                     GASTDSTLDILRGYGERILWVSEKDNGQTEAINKGIAKSTGDIVAYLNSDDLLLPGSL
                     FLVAQIFLDNPKIMWLSGDGWIINANGKKIQLEIKLFKNMLRLFPVMTVLAIANYIIQ
                     PSTFWRRTLHDDIGFFNEDLNYVMDFDFWMRAIKKYPLKVVYKNLSSFRIHDSSKCGT
                     AFEKVFREQQEIVYKHTSNFFIRKLSDLHYMLARFFYKLLKRY"
     gene            15420..15665
                     /locus_tag="PLF29_00525"
     CDS             15420..15665
                     /locus_tag="PLF29_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52462.1"
                     /translation="MKKGEKRRVSIKKVGLNLLYVVPNKVGGTQTYAEGILRSLSEDS
                     SGLEVHVFCSSRYYELLKKTNTVSFFTLLVLILKTAL"
     gene            15575..16552
                     /locus_tag="PLF29_00530"
     CDS             15575..16552
                     /locus_tag="PLF29_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HXK52463.1"
                     /translation="MFKQILRTTKKNEYGFIFHTVGFDTKNRTLRFLYENFILPFIMR
                     KHGVDVLHSLGYTSPFFIPGKNIVTIYDLNWYYQKDSFSRINRLFWKFLVTFSARVAD
                     VIITSSQASRDSLIEVLNIPENKVLAFYPGYTKYSSVDKNIKSIVPEIEGEFILTVSS
                     FLPHKNIMRLLEAFKYVSVATNLQLVIVGSGGKYGEAVSEYLLKNYEKGRVVLTGWVS
                     NEELATLYKKAKAFVFVSLYEGFGFPVLEAFVYGTPVISSSCYSLKEVLGDAAEIVDP
                     YSSESISKGILKVVSSSTLSKKLVKKGHIQCKKFEWPAVSKELKRIYYA"
     gene            16851..17561
                     /locus_tag="PLF29_00535"
     CDS             16851..17561
                     /locus_tag="PLF29_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXK52464.1"
                     /translation="MKLSVVVPAYNEKSTLESILARVKEVDFSDTPELPDIEKEIIII
                     DDCSTDGTTQILQELQKSDPDIVVIFKKTNEGKGFALRDGFRKTTGDYVIVQDADLEY
                     DPNDFKKLLRAVVEDGADVVYGSRFSGTYKDMSNLHYYGNKILTIITNIFFGVLLTDM
                     ETCYKLMPGKFARQVEIKSARFNFEPEITAKILKSGLKIVEVPISYQGRSHNEGKKIT
                     WRDGLSALFTLIKFRFTN"
     gene            17563..19755
                     /locus_tag="PLF29_00540"
     CDS             17563..19755
                     /locus_tag="PLF29_00540"
                     /inference="COORDINATES: protein motif:HMM:NF021379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HXK52465.1"
                     /translation="MFNRKETIKKSFQQAPIIFLVVFCIGAFLITVRINIFRYNNFDL
                     GKFDLGNMAQMAWYTLYGGKFMWLTDLFGANVPRWAMSHVDPILVIFLPLFAAIPHPL
                     TLALAQAAMVLFSSITVYKIAELHLRSKLAALFFGLAFTFYPAIGFLNAWTGYHGVTA
                     AIPFMFLAFYVYERMYKADKYSVYGLSVFWLFLILTMMGKEQIPLYIVLYGMFIVLFR
                     IPISLKISFFDLIRAKQFKKIYSSINVRVGSAMALVGSLWFIVAFFIIIPHYSSYRIE
                     GFNNFALMLSIDPNSATNVDEDNYFLSRYEGFGDSYTDIVLGMLKNPRYLVSVLFGGD
                     KSENLRQTLEPMGYTSLLYAPLFILALPDLLINYSTTAGGIGTSEIINHRISMIIPIL
                     FISSIYGVRYLSDTVIFLFSKSKKQKILSSYLPAALAFFVMYMNISTSFSYQNPVYIW
                     FQQAVAKRINIGFFQEVQAKTDLETIKLSDIKIGDVLRLSELENKDRECAQKIISQIP
                     DEASVSGPDYLGAHLSLRPTYAIFPALYKEADYVIVDVFAQKILRILDLDVTIVRDVV
                     ADVIKSEDYQQVFGCGNLFVFKNVGPHDKNRLLPLQERYEYTETSSIEIFKGLTIVDY
                     MLPSVLKRDSNEQAYFTYVKRTQEGLDDYVLFMTFINKDNGEIYQVANLPSFSLKQLR
                     EWDKGMYYTENIELTIPSFVSPGEYLSFMGMTNNIRTRSVYLGDVVVK"
     gene            19771..>21056
                     /locus_tag="PLF29_00545"
     CDS             19771..>21056
                     /locus_tag="PLF29_00545"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HXK52466.1"
                     /translation="MMGANKNNLEKLQILSLASRLSAMFVGLLQSFFLFRLFSVHDYG
                     VVQIAVAVGSALGIYQHLGLASGSTREIAASKDKNEVFKIFITALSIRYIITFPLAIG
                     LFHYAEYIALDKYNNAALILPLKIYALTLIVQGVQSMLNSVLSGTKRFNALFTYQVAI
                     AVASLVLYLPLVHFYGINGYFYAMFAHNFLGSVALAFIALGPLRENLALPNFREFKYF
                     FRELFTISLSIYLVKIIYTLWERSGTLILGLTISPEAVAVFSAAFLFSKKIIHISDAI
                     TDVNLPYLSEKYVNNIAEFQKAFSDNFNKVFVLILFIGVSAVFWSKQLVLLFASSKYS
                     DALPLILPFMFTFIFYSYLNIIKSSILVPAKLVTQMIATFVILIGFGIGSYYGFLAFT
                     DFSHVSSMTLGLLVGSTLALLFAIYAIYLRLKFKIFT"
BASE COUNT         6150 a         3519 c         4422 g         6965 t
ORIGIN      
        1 aataatgctt taattgaggc agagattaca aaatctttaa tattaataaa aaatacgcac
       61 aatataaagg atcaaaggct tgtatcatta gcatacctta tgagcacacc gtgtctttta
      121 tggccgggtg cgatcttggg ccctcggcag ttttgcatgc ctccgccaat gtcgagctat
      181 atgacgaaga acttcagttc aagccccata tatatggtat tagccacgtt gatgtcgcgg
      241 atttttcaga agaccatgac gcaaatctaa aatatatagg taaagagttc ctaaaacatt
      301 ataaatccgg taaatttata gtttctgttg gaggagagca ttctattacg attggcttgc
      361 ttgagggttt aaagcaggtg gaagaagacg taagtatcat tcaatttgac gcccacgcgg
      421 atttgaggtc tttttatcct ccttcaaaaa aaagccacgc atgtgttatg agaagggcaa
      481 gtgagatgtt tcctaatact ttggcattag gcattaggag catgggggag gacgcggcgt
      541 cctatataaa gaaaaatgac tacccgattt tatatgcaaa agatttattt gaaatgagcg
      601 ataaagaagt tcaggaatat ttgtcgaaag tgattagaaa caaaaaagtt tatataacat
      661 ttgacgtcga tgtatttgat gccagcgttg tatcgttaac gggaactcct gaaccgggag
      721 gtttagggtg gtatcaaact ttacgcatat taaagcatat atttaaaaag tacgacgtaa
      781 tagccgtgga tgtggttgaa ttgataggca gagagtctcc gacaaaatgc gcctttaaca
      841 ccgctctact aattagaaaa atgctaggtt ataaatttct tagctttcaa aattaaaagg
      901 attttcgttt taattcagag agatatattt gacacaacgg ctgtttcttg tcattcttaa
      961 tataaatata taaagctact aacgtgctag ctttaggagt taagttaact gtgccgtata
     1021 gtaaaaaaga tctgctcgca aaacccatcg aacacataga catcactaag ctagaaccta
     1081 cgtcttttgt tgattctatg ggatctatgg gattcacgtc acgggaagtt tcaaacgccg
     1141 cacagatatt tgttgacatg ataaatgacg aatcttgtac tgtaatctta actatagccg
     1201 gaagtacaag tgccgcgggc tgtatgaaaa tctattctga tctgatcaag tacaatttgg
     1261 ttgatgttgt tgtagcaacg ggtgcaagca tagttgatat ggattttttt gaggctttag
     1321 gttttaggca ttacgttcga gaatttgagg tacctgatga tactctaaga gatttgtata
     1381 tagatagaat ctacgataca tatatcgacg aagaacaact tcaggtatgc gatagcgttg
     1441 tgcgtgatat tgcaaactcc attggagaag gagttttatc ttcaagggag tttatagtcg
     1501 aaatgggaag atatttggaa aaatccggaa aaaaggaaga ttcattagtg ctgcaggcat
     1561 ataaaaatca agtgcctgtg ttttgtcccg cattttccga cagcagcgcc gggtttggtc
     1621 ttgttttaca tcaagcagag cgtcctgaaa aacacgtctc aatagattcc gtaaaagatc
     1681 tacgtgagct tacggcagta aagattgcct cggaaaactc cggtttaatg atgattggtg
     1741 gcggggcacc gaaaaacttt gctcaggata ccgttatatg tgctgaaacc cagggaatag
     1801 aggttcccat gcacaaatac gctgttcaaa taactgtcgc tgatgtcaga gatggggcct
     1861 gttcaagctc gacgctttca gaggctcagt cttggggcaa ggtatctctt tctcatcaac
     1921 aaatggtttt cgctgaggca acctccgttc ttcccttgat agcgagctac gcctaccata
     1981 atacttcttg gtcgagcaga aaaagaaaga ggctttcagc aatactctaa attagtttag
     2041 ctgagttttt ttgtttcgca gcctattccg tgcttatttt atgttgcgag taaagtacgt
     2101 tgtgaagaat acaccattta tttatatatg ttctgcttgg ggtatttaag tcctcgatcc
     2161 agttttatta catcaacatc tctaggctgt agggtttttt taaggtactc tacagcactt
     2221 tcaggttcaa agtttttgca tgagtacagg tctacagtag caaaacctct tttcgcgaaa
     2281 gtatgtatac taatatgaga ttcttggata ataacgaatc cactaaatcc tcccggatct
     2341 tttcctccca taaattcgtt gcttgtagct tttattacat agggctctgt catttttttc
     2401 ataccagcaa gatcggcaag atcggatagt attccaaaac atagctccat gttacttaga
     2461 gttttataag gacatccata ggcgtcaaaa attaagtggg gcccaaaagt agtcgtctca
     2521 atgtttttat aggcagtcat accaggaata tatcagagtc tttttctttt tgcaattgtg
     2581 gttatataat tgatgtgtgg actttctctg gcctatcaca tttatagctt cattttcagt
     2641 aatatctctt attgaaaccg aattctacac tctttttggt ttgaaaaagg tagatatagc
     2701 ggaggtcgtt gtgggggctt tagctacagc actttatttt ataagccttt cgttaatgag
     2761 gcattcaagt tttacggtaa gtattttagt gcttattatt ggttggactg ttggaaacat
     2821 tgtattatca accattttct tatatgtgat ggggagtatt aatcacgatt ctaaagtttt
     2881 ttatgaggta attttttgga tagttttaat gatagccgca atagttcgaa tgaatctgat
     2941 tcttggagaa taaaatatgg gaagcaaagt tagcaaaagt tttaaacacc attttttgcc
     3001 gcatccttcc ggaaagcagc gtgccactct tttaactgag gctgcaattt ttacatactg
     3061 tatcgtactg gtcttgtctt ttttaattat aagagcaact cctcagttgg ctcctggagt
     3121 tttgggttat gcttcgaaca ttaattccga agacttactt agatatacaa accaaataag
     3181 gttgcagcac ggattgggag aactaacaat aaaccaagct ctaaatagag cggctgacaa
     3241 gaaagctcag gatatgttta caaataacta ttgggcccat atctctccca caaacgtaaa
     3301 gccttgggat tttattctct ctgagcaata cgactatgct tatgccggag aaaatttggc
     3361 aaaaaatttt gataactcta aagctgtagt tcatgcttgg tataaatctc caagccacag
     3421 agaaaatctt ctaagcccga attattccga aattggcttt gccgtaagaa atggggagct
     3481 ggagggatat cagactacgc ttgtcgtgca gatgtttgga actctacgta atccttctta
     3541 ttcagctcag aatcaaaatc aggattttcc tggtacttct gctgtcgcag aagtaccgtc
     3601 ctcaagttca gggaaaagcg ccgaaaatat ttctcaagta aatgcgcaaa acgaactaac
     3661 attggcgaca ttaaaccaag aaaatattgt ggcaaaaaat gcttcagaaa gtcctcttga
     3721 gccgttagtt tttaagagct cgatgtccgg aaccgatggg caggagaaag ttgttttgtc
     3781 tttagaccta tccctaacag ctaagctgct gtttggaatt ttcggatctt ttatactttc
     3841 attgttgttt ttggacatct ggtattcttc taagaagggt atttcgagag tctccgggca
     3901 cgctctcgcc catataatat tttttaattg ttgtattagt aggggtttct tttgtactaa
     3961 gttcaggcac tgtcctttga tatatcatgc atattatagg taaaagcaac tctcatcatt
     4021 ttatacattt tacagttctc atagttgggc tcttacttac cgttttcttt tttgattatt
     4081 tctcttacag ttttgttata agctcttttg ttctattttt aggttcgcta ttttattttg
     4141 tttggggtat agtacatcat gcaattgagg atcgggttac tgctaaaata gttttggagt
     4201 acttctcgtt ttccgtcttt gtctttgttc tcgttatgat gacaatggct ttataaaaaa
     4261 tgaaaattgt aattgtagtc ccgacttata atgaggtaga aaatgtagga aatttaattc
     4321 cggcgatttg gcaggaaact aaagaacttt ctcacgatgt aagggttctt tttgtggatg
     4381 gaaattctcc agacggtact gcggaaaaag ttaaagaaat gagcgcgctc tacccttttg
     4441 tgagcgtaat acttgaaccc gagaaacgag gtctgggggc ggcttacatt tatgctttta
     4501 aatatgctat gtctgaaatg tcagctgatg tagtagtaga gatggacgct gattttcagc
     4561 acaatcccgc cgacatccca agattaattg ctgcacttga tgccggggcg gattacgcaa
     4621 taggttcaag attcgcaaag gggggctcta tcccatctga ttgggccttg tacagaaagc
     4681 tattaagtat aggcggtagt tatttttcca agatagtttt aggaattttt aatgttaatg
     4741 actttacgtc gggttttagg gcttcacgtg ttcgtggctt tgtagacaaa atagatctgg
     4801 atagtatcct ttctaaaggt tttgcatata aaatagacct tttatataaa atgcataaac
     4861 tcggtgcaaa gataactgaa gttcctatcg cctttggctt aagagataga ggggattcga
     4921 aaatggaaaa agataatttc caagactctc taaaaacggt tttaactcta agatacagac
     4981 aaaacgaatc gttctttaaa tttcttggag tcggattaat tggcctgttt actgattcag
     5041 cactgtttaa tattctttat gcggttagtt tacacgatct cttgcacatg agctcctctg
     5101 tagaaagttt agtgtcggga tttattgcaa tgtttgttac attcaccctg aataattttt
     5161 ggtcgttttc agctcgaaaa ataggttcta agattgagct tgcggctaag ctaggttttt
     5221 actacgcaac atcatattta ccaattgtct ttagaagttt cttaattcat ttttctgagc
     5281 agacctttgg cgagtcattt tttgttagaa acggcgcttt ttttataggt atatttattg
     5341 gattagtgtg gaactttaca atttatagca aggtcatttg gaagaaaaaa tagtgtataa
     5401 ttattgatca tatggaatac accattgtta tacccgttta caacgagtca gataaaatcg
     5461 ccaatacaat aaaccaagtc ataactttta tgagaacttt ttcagagaaa tttgaggtta
     5521 ttgtggttga cgatggcagc tccgacaata ctgtacagat actggaaaac ctcacttctt
     5581 cttataaaga acttgtaata ataaaaaaca agcataaagg taaaagcgct gccgtctggt
     5641 ccgggatgta tcaggccaaa ggcgattata tttatatgtg cgatgcagac cttgctactc
     5701 ctatagagga gttgaaaaag ctagcttcgt ggattacaga gcataactac gacatagtga
     5761 ttgcttcgcg agaaggcgcg ggcgctgttc gtattaacga gcctcttaca agacatttag
     5821 cgggaagaat ttttaatgcg tgggttcaag ctttcgcact tccgggcatt aaagactctc
     5881 aatgcggatt caaattattt agaagagagc cggcaaaggt gatcttctca aagctgcagg
     5941 tttatgggga aaatgcgcct gaaataaatc gcccgcagtt cggagcattt gatgtcgaag
     6001 ttttatatct agcaaaaaaa ttcaagtata aagttaaaga attacctgtt atttggaaat
     6061 acgttaaaac aaccagatta aatttttttg aaaacacatt tagaatgtcg accgatgttc
     6121 taaaagtaag aataaatgac cttcttggaa ggtataagta ataaatttag ggcccgcttt
     6181 gataaagagg cttttcttaa aaaatcgccg gtgtaaaggt cctaactaaa ggagagttta
     6241 atgaaaaaag tatgtgttat aggtacagga tatgtaggtt tagttggtgc tgctgtgttc
     6301 gctgactggg gcaacagcgt tgttggagtt gatatagatg aaaaaaagat agctaagatt
     6361 gaagagggta ttatgcctat ttatgagcct ggtcttgacg aggtggtgct gagaaatatc
     6421 aaaacgggca aactttcgtt tacaacctct ttagctgaag gcatgaagga cgcagacatg
     6481 attttgatat gcgtaggtac tcctatgtcc gacaccggag ctgccgattt atcctatgtg
     6541 tgggctgtgg caaaagaaat cggtcaaaat cttaagaaag gtgataaata cgttgttatt
     6601 ataactaaaa gtaccgttcc tgtaggtact aatgagcgtg ttaaacaaat agttcgagag
     6661 tacgctgagg aaggcgttga gtttgatgta gcctcatacc ccgaattctt agcggaaggc
     6721 agagctttgg aggacatgaa ggagactgat cgaactgttg tgggaacaga aaatgagcgt
     6781 gctttaactg ttttacgtga attttatggt catttagatg ctcctatggt tgagtgcgac
     6841 cttagaaccg ctgagatgat taagtatgca tcaaacgcta ttttagccac taaaatctct
     6901 tttattaatg aaatcggcca gctttgcgag cgcgcaggag cagacgttgg tcttgttgct
     6961 aagggcatgg gttacgataa aagaatagga agacactttt taaatgtaag cattggttat
     7021 ggaggaagtt gcttcccgaa agatgttgct gcattgtata aaacttctac agaggaggca
     7081 tacgatttca aattattgag aagtgttatg gaagttaatg actcgcaaat gcaccatttt
     7141 gttagaaaaa taagaaaagt gtttggaatt aatatggaag gtaaaaagat agcttgcctt
     7201 ggtctcgcat tcaaagcaga tacagacgac attagagatt ctaaggcaat aaaaattgtt
     7261 cgagagcttc gaggtcatgg agcaactgtt tcggtttatg acccgcaggc aatggaaaac
     7321 tcaaggcgcg aacttgggga taaagacatt acctatgccg aagacatgta ctcagctgta
     7381 aaagatgttg atgctgtttg tatactaacg gaatggggtg agtttaaaaa tatagacata
     7441 gagaagttgt ggcgtgcaat gggctcatca aagagaaaat ttgttttcga tggtcgtaac
     7501 cttttggatc aaaagaaagt cgaagctcag ggctttacat attttgctgt aggaaagaga
     7561 acaaacggat acaacgaatt tttacttgag gacgaagaga agagaggatt tgttacaacc
     7621 gctttattaa agagccagaa tgggtaaaaa tagttcttat gagtaaagat caaaagaaga
     7681 aaattctata tttcgttccg cagtttccga tgctgtctca gactttcatt agtcgtgaga
     7741 ttggaaagct tgttgaatgg gataatcttg atatttctgt tctctcaatg tctagaggta
     7801 caggttttgc ttcgcagaaa gttttggcaa aaactcacta tcaaagattg tcggtgaaga
     7861 tttgctttga ggcgctgata aaatatattc tgttacgccc cagaaaaacc tggcaagccc
     7921 atagactgat ctcaggtgaa gattctgtgc cttatttgtt gcaactaaaa agaaaaaatt
     7981 ttgatccggc ggcggataac agcgtcttaa aaagattcca tcgtgcaaga ttagtccatt
     8041 tttttaaggg agtggctttt gcgcgaatgt ttgaggttta caagccggag cacatccatt
     8101 gtcactttct gtcagatcag tcgacaattg taatggttgc cgcaaagtta ttgggggtgc
     8161 cctttagtgt ttctgctcac gcgaaagacg tgtttgttga agggtcgcta attccagtaa
     8221 aagcaaggga ggcaaaattt attgttgttt gcaacggaaa cacatggcga aaaaccatcg
     8281 agttcgcaaa tctctcggcc tctagcggta atgttcatct gctgtttcac ggctttgact
     8341 ataaagacgc ttattctaaa ccattgtccg tagaaaagcc tgcaataacg tctatatttt
     8401 taggcggtac tcgatttact aaaaaaaagg gaattattta catggttgag gcttccaaga
     8461 tattgaggga tgaaggtttt gctcatgttg taaatctggt agggcctgca gatgatcttt
     8521 ctacctacaa cgagattaag gataaaatta aagaatacgg tctcgaagag accgtaataa
     8581 ttcacggtga cggcaaaggc atgccaaatg aaaaagttct ggaattttac agaatttcgg
     8641 atatatttgt atttcctggc attgagacaa aagaaggcga tgtagatggc gtaccgactg
     8701 tgttgataga ggcggcactt gcaaagttgc ctataatttc cacagatgta ggaagcgttg
     8761 gagatttaat tgactcaaca aacagcatta ttattcctca aaaggatgtc ggtgcaatag
     8821 ttgacggggt gaaaaagcta acagcagata ctgcattgtc taagaaactg ggtgaggcgg
     8881 tttatattaa agcagagaaa ctctttaaca tagatataaa tgtaagacag cttgagcaga
     8941 tgcttttgga ttagtatttt ggccacaagc tatttttttt aagtccaaag cccggaaccg
     9001 ataacggtag aagcaaagga tccttatagc cgagatccaa aaatatcaac cggggtaaat
     9061 taagagtgca aaatcttaga ccctgcttca attttctttt gagggctctt ctaatttttt
     9121 aagaagattt tttgcctgca attttcgatt agccagtacg ccggctgcat cgggacagtt
     9181 tattactgcc gataggtaaa ttctggctgt agtattttta ttggtcttta tgtgcagaag
     9241 agccatttgt agtgctattc ccgttgtcca taccgataga accatgtcgg cttgtttatt
     9301 cttattttgt tcttcttcgt agccatagta aatacaagac tccgcttctt gaagcacttt
     9361 caatgctttg tcgtaaagtt tgagctctat gagtatttta gcttttatag gcagtggcca
     9421 agctctgtgt gcttgtgatc cgggcaaggt ttttaaagcc tcgtctacca tttttagcgc
     9481 tgttttaagt tttttctctt tagatttagt gtcgtgaagt aaagccaggt cgtaatttaa
     9541 tgaagcttga tgaactttca aaattgatat gctctcacct tccgttgctt ttaaagcctc
     9601 ttcaagacat ttactagctt ctgttagata ttttagcttg cctttgtttg ttttttctct
     9661 gtcggcaaga agtgtgtagg ctattttttt atgatcgttt gtctctctaa tacctttagc
     9721 gtatttattg gcgatatccc atgcctgaac cgcgtctagc attgtgagtg caaccataat
     9781 gaagccctgt tctcttatag ttacagactg ctttaggtag ttgtctctgg tttctttctt
     9841 tgcatttgtg aaaagtcgta gtgttgagtc tgcggatgtc attttggtaa taattttagc
     9901 tacatcttcg ggggaaagtt ccagtttttt aattgttgcg gggctaagcc ctgttttttg
     9961 aagatataaa ttatagactt caagctcttt ttttcttatt tcgttattat ttaaggccat
    10021 agtactttga ttttacacta acaggcctta tttttaaaga ttactcggcg gtgttagtgc
    10081 ttgagcctct ttgtcacatg ggaatgatag caattagttc gtattttttg atagaattaa
    10141 catatgcata tagcagtctt taccaaaaaa accactttcc ataagggata tggaggttta
    10201 gagacccaaa ataaattact tgttgaggaa ttggttaggc gaggaaattc cgttattgtt
    10261 ttttcaccga aagccgagat tgagactgac ggcactgttg attcaggggt tacttatgtt
    10321 tttattcctg ccgtgtatcg tatgggcata ttctttcaaa atattgaaga tgatgtcgca
    10381 aggttttttg agaaattttt accatttata aatgtaagcg ggctgttctc caaaaaagga
    10441 aaagaagatt ggaaagagaa atcattagcc tatttttcta agtaccatgc tgagaataga
    10501 tttgatgtgg ttatatgtca gagttctgta ggcttgggaa tcataaatgc taaaaaacag
    10561 cttggaataa aaatagtgtc aatttcgcac ggatcgattt tgagtgaata tgtcacaaga
    10621 gttaaggagc ttgagattaa atcttatttt tcacccgtcg cgctgctttc tttatttaga
    10681 gataccgctt atgttttagt aaattatttt acaaaccagc ggcgttttat ttttggttcc
    10741 gataaaatta ttgctgtatc gaatagcgta aaaaaatcag taattgacga gacttttatt
    10801 cctgaagata gggtagttgt aatacacaat ggggtagagg ccggcttatt tgataaaagt
    10861 ttgcccgcca aaaacagctc tctttattcg aaatctgcta ataaagatat ttcgagcttg
    10921 gcagaatatg atggctatgt gaaccttgtt tttaatggca gaattgaaaa aacaaagggg
    10981 gtttttttat tgttaccctt tgtcgaagaa ttgataaacg atagcgaaat taatgttcaa
    11041 ctaaaaatgc atgtaattgg agacggcaag gatctagctg ctttaaaaga taagattcaa
    11101 aaatatgatt tacaggatat ttttgttttg catggcaaac ttcaatacga acaggtagtc
    11161 agcttactta aaaacatgtc aatatttatt tttccgacat tgcgtaaaga gggattcccg
    11221 atggttttag ttgaagccat gatgtgcggc ctgccttgta tagctagcga tattggaggt
    11281 aattcagatg ctgtggtgtc gggtgaaaca ggttttttgg ttagccccgg aaacatttcc
    11341 gagcttgtag aagcttgcaa agccttactt ttagacaagt ctttatccgc aaaattttca
    11401 gatcgtgctt tgacaaaggc aaggtctgag tttacattga ataacatggt tgacgggtat
    11461 ctgaaagtca tcgaggaggt tcttcaatga agattctaag aatcatatat gattggccac
    11521 cgccttctga cggcttggcg ccacatccct atgaattgtc aaaagcccaa attgatctgg
    11581 gtcacgaggt gtcggtattt tccgcacgat ggccacggca aggccctcct gccgattaca
    11641 gcggtaagat aaaactcatc cactttttag gtaattcttc attacctttc cgcacaccgg
    11701 ctcaaggact aatgctagtt actgtcgctc ctctgttgtt ttttagatat tggttttggc
    11761 gtttgaaaaa gtccaattct gtagatgtta ttcatagtca cggacatttt gcactgtgga
    11821 tttacctgta tagatatttc ttacgaaaat tttataaaag agcaaaagaa ctaagaactc
    11881 ctcttgtagt tcatttccat aacacagtgg ccggcaggtt cgaggctatg aataaaagcg
    11941 gcaaagagct taaatatttg acagagaaaa ttgactggcc gttagcacgt ttgtctgatg
    12001 aaacggctgt taagatagca gatgcatgta tctttgtaag cctagatgtt tttaatgagg
    12061 ccaaaaagca ttacaatgtc gacgcaaaaa aatgttttat tttagagtca ggagtaaatc
    12121 cggatttatt taaaccgacc actattgaag aaaaggataa gactagaaaa gatttaggtt
    12181 tagatccaaa agatgtagtt attttgcacc atggggcaat gatagatcgg aaaaacactc
    12241 attgtgttgt tgaggcatta aagtacctgc ctccccacta caagctttta ctggtagggc
    12301 cctggcccga ttctaagtat cggcaaacgg tcgatttgat tattgaagaa aacaggcttt
    12361 caaaaagaat tgtatttgtt gggtatactc caaatgaaga ggttcctata gcttaccagg
    12421 cttcggatat ttttgtgctc ccgtcgtctt gggaaggttt gccaaaagtt gctgtgcaag
    12481 gccttgcgtg cggcattcct gtccttggct cggcttttaa aattgacgat tctgtctcag
    12541 gagtttttta cttacacgat accaagcctg agaccattgc aagacaaatc aaagagatta
    12601 ttgaaggcag gccttttgtg gacgtggatt tagtgtctcg catttattct tggagacaga
    12661 gggctttaca acttaataag gtgtacgatt ttgtttcaac cgcaagaagt aaaaatactc
    12721 gtatatgaat atgaaaaaac taccttttat agttttggct ttcgtcatac ttatacgagc
    12781 cttatttacc aacgtaaacg cggcagaatg gggcgatagc tatcgaattt tgcgagcagc
    12841 ggagtattta agaaattatc aatacccttc agacgaaaaa agagccccgt tattttcact
    12901 tattttagct gctcgccctt ctaaagttga ccctgtgcaa tgggggcggg cggaaatgtt
    12961 agttattaca gctttgctct taacagtttt tacgtactat gttcgttatt ttatttcgag
    13021 tccggtttat caatctctat ccatcgcttt attaggtttt aatcctgtgt ttttttattg
    13081 gtctcttaga ataatggccg atgtgccttt tttactccta gttttatgct ctttttattt
    13141 actaaagcgg caatcaacgc ttggaaggtt ttattggcaa aaatctttta ttttggggat
    13201 tttaggcgca ttaggcgttt tagttcgttt tgagggctac attcttattt gcgcattgtt
    13261 tattggcatc atatttttgg aacgcggatt ttattcttgg gaagagacta ttttacaacg
    13321 aaaaaattta cagaaaacac ctagtgcagg taaaaagagg cgcacttcaa aaattccagc
    13381 cactgtgaca aatttgaaaa atattataag atttgttttt tctcagctta atctcatttt
    13441 taatcttttg agaaagaaaa atagagagat tcttgctttt gtaacggcac agctttttat
    13501 actggtgccc tactggtatt atagaaatcc cctatcttcg tcatattttg aagaaccttc
    13561 aggaagaagc tatgatttga ataccttttt gatttatttt gcttcgctgt tttttatatc
    13621 tggatttgtt ccttttttta gctatatttt ctttagacct gccgcaggaa gtctaagctc
    13681 aagcacgact tttgttgaga aagattctaa gtcgagcagc actttatcat tttcggctga
    13741 ggctcgtgca actataaaga agaaccttaa agatgttatc tcaataggta acgaaaactt
    13801 aggggtctcg gcttttgtta ttgccgaact attgttagtt ttaatttggc ccgcagcaat
    13861 accaagaata tttacgccgg ttctcccatt tctgattata ttgctgacaa taaatttaca
    13921 taatggtctt gagaatttta gatcggttat gaaagatagt aagaaaatgt ttttattttt
    13981 tctttcatct cagttttttt tacttttttt atatgttgtt ttgcagttta aataccgttt
    14041 acagtttctg cttcctataa aggaagtttt tatcgtaatg cttgtattac aggtattgct
    14101 tgttttttta caattgctaa aaaataaaaa tgtgtttctg gtgtctttgg catttgttgt
    14161 ctttgggtgg tctctctcta ctgtttttct tcataaagat atttttaaag ttgtgcggca
    14221 ggcaaatgtg tatgcgacgt ctaatctaaa aggtacggta ggttataatg acgtaagcag
    14281 tgtcagtgat tggtatttaa atcaggccgg cttgaataag tctatctcgg gtagattttt
    14341 tgatttaact aagaagagta atagagagta tgacgtatta cttgagcaag gatttgacta
    14401 ttttcttgtc actaacgagc ataatacaga tttaactatt gacttatcgt cttggccaca
    14461 tttaaaggtt ataaaaagtt tttcaagtaa tataaacggc aaaacattct tcactaacat
    14521 tattgagttc gaaaagtgac attagatgcg ctgacttgct tttcttattt ggcatcgggg
    14581 gtggaggtag gttttccttc gtaatgctcg gaaacaaaat ggtaaaattg atcaatgagt
    14641 aggtatagag aatatcctaa aatttcaata ataactccaa gctataacca aggccgcttt
    14701 attaaagaaa caattgaaag tgtgttaagt caaaactacc ctaatcttga gtatattgtc
    14761 gtggacggtg cttcaactga cagcacactg gatattttaa gaggctatgg tgagaggata
    14821 ttgtgggtat ctgaaaagga caatggccag actgaggcta taaacaaggg catagctaaa
    14881 tccaccggcg acatagtggc ttacctaaac tctgatgatt tattacttcc cggttccttg
    14941 tttttagtag cacaaatttt cttggataat ccaaagataa tgtggttgag cggtgatggc
    15001 tggataataa atgctaatgg caaaaagata cagctcgaaa taaagttatt caaaaatatg
    15061 ctgcgccttt tccctgtaat gactgtttta gcaatagcaa attacattat tcagccaagt
    15121 actttttgga gaagaacctt acacgacgat ataggttttt ttaatgaaga tttaaattat
    15181 gtaatggact ttgatttttg gatgcgtgcg ataaaaaaat acccgcttaa agttgtatac
    15241 aaaaaccttt cttcattcag aattcatgat agctccaagt gcggaacagc atttgagaaa
    15301 gtatttaggg aacaacagga gattgtttat aagcatacta gtaatttttt tattcgtaag
    15361 cttagtgatc tgcattatat gctggcaaga tttttttata aattactaaa gagatactaa
    15421 tgaaaaaggg tgagaagagg agagtttcta taaaaaaggt aggtttgaat ctgctttacg
    15481 tggttccgaa caaggttggc ggtacgcaga cctatgcgga gggcattttg cgaagtttaa
    15541 gtgaagatag ctccggctta gaagttcatg ttttttgttc aagcagatat tacgaactac
    15601 taaaaaaaac gaatacggtt tcatttttca cactgttggt tttgatacta aaaaccgcac
    15661 tctaagattt ttatatgaaa attttattct gccttttatt atgaggaaac atggggtgga
    15721 tgtattacat tcattaggtt acacatcacc tttttttata ccaggaaaaa acattgtaac
    15781 tatatacgat ctcaactggt actatcaaaa agattccttc tccagaatca atcgtctctt
    15841 ttggaaattt ttagtaactt tttcagctcg agtagcggat gttatcataa cctcatctca
    15901 ggcttcgcga gattcgctta ttgaggtgtt aaatattcct gagaataagg tattagcttt
    15961 ttatcccggc tacacaaaat attcaagcgt cgacaagaat atcaagagca tcgttccaga
    16021 aatagaaggt gagtttattc taaccgtatc ttcattttta ccacacaaaa atatcatgag
    16081 gttgttagag gcattcaaat atgtaagtgt tgcaacaaac ctccaacttg tgattgtcgg
    16141 ttctggagga aagtatggcg aagcagtctc ggaatatctt ctcaaaaatt atgaaaaagg
    16201 cagggtggtg ttaaccggct gggtttcaaa cgaggagttg gccacattgt ataagaaagc
    16261 gaaggctttt gtatttgttt ctttgtatga gggattcggg ttccctgttc tggaggcttt
    16321 tgtttacggt actccggtaa tttcttcttc ctgctattca ttaaaggagg ttttagggga
    16381 tgccgcagag atagtagatc cttattcgtc ggaaagtata agtaaaggta tattgaaagt
    16441 tgtttcgagt agtactttaa gtaagaaatt ggtaaaaaaa ggacacattc aatgtaagaa
    16501 atttgagtgg cccgctgttt caaaagaact taaaagaata tattatgcat aaagtacaga
    16561 ttaatataat ctacacataa tctatcctgt tggttacaaa gaatattgaa taactttcgc
    16621 gttgttactt atcctaccga gtcttttact ttttggggtc ggcaacttgt aaagacattt
    16681 ctgcggataa tttatactaa aaacattact aagatctcag ggctagtgag tctgccgcta
    16741 aacatggggc ttctttttga aaggttttat tactagggcg ctgtagtgtt agaagtgttt
    16801 tcgctaaatc ttttgttggt tagtgcgcgt aaaaaatgta taattatgta atgaaacttt
    16861 ccgttgttgt gcccgcttac aatgaaaaaa gcactcttga gtcaattttg gcccgtgtta
    16921 aagaagttga tttttctgac actcccgagt taccagatat cgagaaggag attatcataa
    16981 tagatgattg ttctacagat ggtactaccc aaattcttca agaactgcag aaatccgatc
    17041 ctgatattgt tgtgattttt aaaaaaacta acgagggtaa agggtttgct ctgagagacg
    17101 ggtttcgaaa aacaacaggt gattatgtta ttgttcaaga tgctgatttg gaatacgacc
    17161 cgaacgattt caaaaaatta cttagggcgg ttgttgaaga cggtgcagac gtagtttatg
    17221 gatcccgatt ctctggaaca tataaagaca tgagtaattt acactattac ggtaacaaaa
    17281 ttttaactat cataactaac attttttttg gagtattgct tactgatatg gaaacctgct
    17341 acaagctcat gcctggaaag ttcgcaaggc aggttgagat taagtcggcg cgttttaact
    17401 ttgaacctga gattacagca aagatactta aatcaggcct gaaaatagtt gaagttccaa
    17461 taagctatca aggacgttct cataatgagg gtaaaaaaat aacttggaga gacggtctct
    17521 cggcactgtt caccttaatt aagttcagat tcacgaatta acatgtttaa tcgtaaagag
    17581 actatcaaaa aatcatttca gcaagcccct ataatatttc ttgtggtttt ttgtattggg
    17641 gcgtttttaa taacagtcag aatcaatata tttagatata acaattttga tttaggtaaa
    17701 tttgatcttg gaaatatggc gcaaatggct tggtacacat tgtatggagg aaaatttatg
    17761 tggctaacag atttatttgg agcaaatgtt ccgcgatggg ccatgagcca tgtagatccc
    17821 attttagtta tttttttacc cctttttgct gctatcccac atccccttac tttggcttta
    17881 gcgcaagcgg caatggttct tttttcaagt ataacagtct ataaaatcgc cgagctacat
    17941 ctaaggtcaa aactggccgc actttttttt ggtcttgctt tcactttcta tcctgctata
    18001 ggttttttaa atgcctggac cggttatcat ggggtaacag cagctattcc ttttatgttt
    18061 ctggcttttt acgtatatga acgtatgtac aaagccgata agtatagtgt ttatggattg
    18121 tctgtgtttt ggctgtttct catattaact atgatgggaa aagagcagat ccctttatat
    18181 attgttttgt acggtatgtt tattgtactt tttcgtatcc ctatatcttt aaaaattagc
    18241 ttttttgact tgattagagc aaaacagttc aagaaaattt attcaagcat taatgtacga
    18301 gtgggttctg cgatggcgct ggtcggatct ttatggttta tagtagcttt ttttattata
    18361 atccctcact attccagcta tagaattgag gggtttaata atttcgcgtt gatgctgtct
    18421 atagacccga attcagcaac taatgtcgac gaagataatt attttctaag cagatacgaa
    18481 ggttttggag attcttatac agatatcgtt ctgggtatgc ttaagaaccc cagatattta
    18541 gttagcgtgc tctttggggg cgacaagagt gaaaacctaa gacagactct tgagccgatg
    18601 ggttatacct ctcttcttta cgcaccgctt tttattctgg ctcttcctga tttattaatt
    18661 aattattcta ctactgcagg gggaataggt acgtcagaaa taattaacca tcgcatttcg
    18721 atgattatac ctattctttt tatttcaagc atttacggtg ttcgatatct tagtgacacc
    18781 gtgatttttt tattttcgaa atcaaaaaaa cagaagatac tttcttcgta cttgcctgcc
    18841 gcgttggctt tttttgttat gtacatgaat atttccacct catttagtta tcaaaacccg
    18901 gtttatatat ggttccaaca ggctgtggct aagagaataa atataggatt tttccaagaa
    18961 gttcaggcaa aaaccgatct agaaaccatc aagctctctg atattaaaat tggtgatgtt
    19021 ctgcgacttt cggaattgga aaacaaagat cgtgagtgtg cgcagaaaat tatttcacaa
    19081 attcctgatg aagcttctgt tagtgggcct gactatttag gtgctcactt atctttgcga
    19141 cccacctatg ctatattccc cgcgctatac aaggaagcgg actacgtgat tgtcgatgtt
    19201 tttgcacaga aaattttacg aatcttagac cttgacgtga ctatagtaag ggacgttgtt
    19261 gctgatgtaa taaaaagcga agattaccag caggtattcg gatgcggaaa cttatttgtg
    19321 ttcaaaaatg tcggccctca cgataaaaat cgtctcctgc ctcttcaaga aagatatgag
    19381 tatactgaga cttcttctat tgaaatattt aagggcttga cgattgtcga ctatatgctt
    19441 ccttcggtgt tgaaaagaga ttcaaacgag caggcttact tcacttatgt taaaagaact
    19501 caagaaggtt tggatgatta tgttttattt atgactttca tcaataaaga taacggggag
    19561 atttaccaag ttgcaaattt gccgtcattt tcgctgaagc agctaagaga gtgggataag
    19621 ggtatgtatt atactgaaaa tatagagctg actattccgt cttttgtgtc cccaggcgag
    19681 tatctttctt ttatggggat gacgaataat attcgaacaa gaagtgttta tttgggggac
    19741 gtagtagtaa aatagctttc gtaggagtca atgatgggtg caaataaaaa taatctagaa
    19801 aaattacaaa tattgagctt ggcatcgagg ctttcggcta tgtttgtggg cttactgcag
    19861 agcttctttc tgttcaggct tttttctgtt catgattacg gcgtagttca aatagctgtt
    19921 gctgtaggta gcgcactagg catctatcag caccttggtc tcgcttccgg aagtacaaga
    19981 gaaatagccg cttccaaaga taaaaacgag gttttcaaaa tctttataac agcactttca
    20041 atcaggtata ttataacttt tcctctggct atagggcttt ttcattatgc cgaatacata
    20101 gctcttgata aatacaataa cgcagcgctc attctgccac taaagatata cgcactgaca
    20161 cttattgttc agggtgttca gagcatgctc aactctgtac tttcaggaac aaaaagattt
    20221 aacgcccttt tcacatatca agtcgctatc gctgttgcta gtctagttct ttatctccca
    20281 cttgtgcatt tttatggtat aaacggctat ttttatgcta tgtttgctca taattttctt
    20341 ggatctgtcg ctctagcttt tatagctttg gggcctttga gggaaaattt ggctttgcca
    20401 aattttcgcg aatttaaata cttctttaga gaacttttca ctattagcct ttcaatttat
    20461 ttggtaaaga tcatttatac tctctgggag cgctcgggaa cgttaatcct tggtttaact
    20521 atttctcccg aggctgtggc ggttttttct gcagcatttt tgttttcaaa gaagatcatt
    20581 catatatctg atgcaattac cgatgttaat ctcccgtatt tatcggagaa atatgtaaat
    20641 aatattgcag agtttcaaaa agctttcagt gacaatttca acaaagtttt tgtacttata
    20701 ctatttatag gagttagcgc agttttttgg tcaaagcagc tggttttgtt atttgcgagc
    20761 tcgaagtact cagatgctct gcctttaata ctgcctttta tgttcacatt tatcttttac
    20821 tcttatttaa acatcataaa atccagcatc ttagtaccgg caaaattagt aactcaaatg
    20881 atcgcaactt ttgtaatatt aattggtttt ggaatcggct cttactatgg atttttggca
    20941 ttcacggact tttctcatgt aagctcaatg acgctcgggt tattggtggg ctcaacttta
    21001 gctttgcttt ttgctatcta tgctatatat ctaaggttga aatttaagat ttttac
//
LOCUS       DAOSWN010000004        20516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_1634_length_20516_cov_4.446019, whole genome shotgun sequence.
ACCESSION   DAOSWN010000004 DAOSWN010000000
VERSION     DAOSWN010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 20516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20516
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1634_length_20516_cov_4.446019"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /gene="nusA"
                     /locus_tag="PLF29_00550"
     CDS             <1..464
                     /gene="nusA"
                     /locus_tag="PLF29_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006211290.1"
                     /note="modifies transcription through interactions with
                     RNA polymerase affecting elongation, readthrough,
                     termination, and antitermination; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HXK52467.1"
                     /translation="VGSCVGQKGVRVQAIISELFGEKIDIIPFSASIEKYIAAALSPA
                     RVTGVEIIEDENRAVVSVPEDQQSLAIGREGQNARLANKLTKWKIDIKGTKGVFDTTE
                     DTSSQSTENRDVVGVWDAAIEKVNKQLEDSAKTTEEKIQENTESEKTESTQ"
     gene            593..2089
                     /locus_tag="PLF29_00555"
     CDS             593..2089
                     /locus_tag="PLF29_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HXK52468.1"
                     /translation="MSTTNKTNTKSNKAVRPPIVTIMGHVDHGKTSILDYIRKTKIQE
                     REYGGITQHIGAYQISHKSKLITFIDTPGHAAFSQMRSRGGKAADLVVLVVAAEEGVK
                     PQTKEAILHAKAAGVPIIVAINKIDKPGADSQKVKQELAQANIMVEDWGGDIVCVETS
                     AISGKGISELLDAILAVAEMENLVADSNAELEAIIIESKLDPKRGVVVSCIVKSGSLK
                     VGSEVTGSGIEAKVKSMVNPYGISIDIAGPSTPVEILGFKTTPHIGDLIVEKGSELAE
                     LSIVDDHNEVIGANTKRTVGIIIRADTQGTLEAVKQSLANLITESVGLDFSIKFVHTA
                     TGDITDSDVLLASTTDSVIVGFNVKEGKSVTELASELKVTAKTYKTIYDLIDEIKDLL
                     EGTAIKEEQKIKGRAQVIKKFKLPSGDVILGSKILAGKVKENDRVGIFLINPSEATEQ
                     DTPLYYSKIKKIKVQKNEVKEIKKGLECGLLLKPQIDEIEPGMYIEVL"
     gene            2181..2840
                     /locus_tag="PLF29_00560"
     CDS             2181..2840
                     /locus_tag="PLF29_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52469.1"
                     /translation="MQIENYDNNQIAELLRDAKSVGIIASRLNQPDTFCAASGLYLML
                     KNTFEDKEIKLIYKGDIPTECSNLINSEDVHANVDERDLVVTVNYKGTSASKVQYSTE
                     DGVFQFRLGPVPKYFDLGNIQSVLSGFDFDVVVTIGIPDLEVTGSLYSSLKHELSSAK
                     IVNIDNTGLNKRFGSVNVIDTQASSLSTLVFNLAAEWKLKPTAKSAKMLLVGVSSTSI
                     D"
     gene            2880..3140
                     /gene="rpsO"
                     /locus_tag="PLF29_00565"
     CDS             2880..3140
                     /gene="rpsO"
                     /locus_tag="PLF29_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HXK52470.1"
                     /translation="MALDKKNKTQLIQGSRLHDTDTGSPEVQITILSKRIAELSEHLK
                     KHKKDNHSRRGLLQMVNKRRRLLAFLKKKDPERFEKYVEKHE"
     gene            3414..5648
                     /locus_tag="PLF29_00570"
     CDS             3414..5648
                     /locus_tag="PLF29_00570"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HXK52471.1"
                     /translation="MKQVKKEIDFNGKKITLETGKLAPQANVSIKATYGETVILVTAV
                     SGGQNPDLDYFPLQMSYEEKLYASGTIKTSRFVKRDGRPTDDATVTRRLIDRAIRPLF
                     PDDFSDEIQIIMTVLSLDEDSDPAFLAGLAASAALQASNIPFEGPMIPVKVGYADQKY
                     ILIPTRKQTEEDSDLNMMVSFSGTDRKFLSIDASANILPEKVILGAIEFARDNAEPLL
                     NLVMDFAKEYNPDGTKYTYISSKPSDEVINSVKAIVKDELATLIKGNYKYNEALNNQK
                     SMLVEKVFTELEGKFKKSEMVKALEKIEKNIARALIMESGKRIDGRSESDLRPISGEV
                     GLLPRTHGSGLFNRGLTQALTVATLGNPSLELLIQDMYGEKTKRYLHYYNFPPFATGE
                     VGRIGGANPREIGHGMLAEKALVPVIPSKEEFPYTILLMTETLSSNGSSSMAATCGST
                     LALMDAGVPIKGAVGGVAMGLMANEDQSEFMILNDLSGYEDSVGYMDFKMTGTKEGIT
                     AIQVDIKLKGLPMDLIPRIIEKSREARLEIIEKIEEIISSPRDSLSKYAPKMCVVKID
                     PTKIGMVIGSGGKTIKEIQEQTNTELGIDDDGTVFISGESEEDVARAQGMVEGLTKDI
                     EVGEVYEGTVKEIVDFGAFVEILPNKQGLLHISEITDDYVEKASDHLKEGQVVTVKVI
                     ETGPNGKVSLSMRAMNPGYEPKPRHSDRGGRSSSGGHSGRDRNSHNSGGGRDRGGRNF
                     RDRR"
     gene            5706..6806
                     /locus_tag="PLF29_00575"
     CDS             5706..6806
                     /locus_tag="PLF29_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXK52472.1"
                     /translation="MPDKNFSIADTPNLSAADYAFAELRTLSSLVNDTRDIPEDLKDR
                     LNQMLDRLDRMAKLGHYAQEFDTLSRYIETIAEIPWGEKTKDNLDLTETKNILDKNHY
                     GMEWVKERILEYLATIKLLKDRQKEGVGKSPVLLLVGLQGIGKTTLAMSVAEALGRKF
                     IRISMGGIGSTLELRGRSKAYPEAEPGQIIKALIKTGVKNPVILLDEIEKASGESGLR
                     SDIMAILLEILDPSQNNSFRDHYIDYPIDLSDVLFICSANNTGTITTALMDRLEIIKM
                     PSYTDAEKATIAKDYLLPKVIELSGLKPEELEIDPALWPSIVRPFGFDSGIRSLSRTL
                     EAICRKVAKEIVEGKTKKVRVDESNLKYYLPS"
     gene            6842..7453
                     /locus_tag="PLF29_00580"
     CDS             6842..7453
                     /locus_tag="PLF29_00580"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HXK52473.1"
                     /translation="MSQNTEKLQIIEREIRKCEKCSLCKTALQAVPGEGSSTAKVVFI
                     GEAPGATEDKTGRPFVGRAGNLLEKLLEKINLSREEVWIGNIIKHRPPKNRDPLPEEI
                     KACRGYLENQLAVIKPSLIVTLGRFAMNHFYPEGKITKDHGKLIKLHNFNVFPVYHPA
                     AALRNRSFAEALAKDFLNIPNVLTELSKNEKSTNNTMMAKPLL"
     gene            7491..9200
                     /locus_tag="PLF29_00585"
     CDS             7491..9200
                     /locus_tag="PLF29_00585"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HXK52474.1"
                     /translation="MNTNNFNSVNSIQPELRIIGFSGAEGATKNMTVYESGTDIIIVD
                     CGIGFPDDELRGVDVVIPDFTYILENIEKVRGLFVTHGHEDHLGAIPYLLKEANIKIF
                     TTPIVQGFIRSRLLDRGSQKIHDDVSFYTMVPGGEAVTLGNFSVSAFHVNHSVPDSVG
                     LAIKTPQGTVLHMADYKIDLAPVIDKPMDFAAISNFGKEGVLCLLSDCLGANKEGHSK
                     YEQSMKGVFTDLYEKGEGKQIIITTISSNISRMYQIMDAATKSGRKVVLLGRSINNSV
                     EIARGLDMLPFSDELFVSHRDSMKHSQSELVYIAAGCFGQQGSALDRISRGEHRDVTL
                     EEDALVIFSADPAPPGTRIPVERLMDQLTLKGAEVIYTKIQDNLHVSGHGPKGDLELV
                     ASMVNPKYFIPIGGSVTQMRAYTSMVKGLGYSTDQVFEMLEGEILEFTENGALMLDEK
                     IDVKQVYVDGTDVGEIGEVVIKDREILANEGVFVVVVPYSKTDKQVKGNSDVITRGFV
                     YVKNSKQLMGTAKDLVNKIIDKSSTDEPWTKLQAKIEQEVSKLLKKETGRDPMVIVHA
                     LYV"
     gene            9220..11481
                     /locus_tag="PLF29_00590"
     CDS             9220..11481
                     /locus_tag="PLF29_00590"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HXK52475.1"
                     /translation="MSARRGRKKKFKLKFNIRGETVRSIAALLLILLAGVVLVSFAAG
                     VVSVDSSIQRVLKILFGYAHLALVPIFLITGLLLIDSIDWRFVEGRVLAGFSILLFGA
                     SGLLHAFYLGDGYEAAKAGKGGGIVGYKIFSVLSSNTSSLGAILVLLTLVIISILLIW
                     DKPIEELAAKLSALGLPFSKLKAAGLERKSKNASTHQKEDIHITSGLNLNFSDNTGYN
                     ESIESSPEEDATNYHDEDSAAEEIEIIPSLAEPKSKNSHEFKTNPKVTDTLAPAPLPY
                     SDRVWENPPLDILNDPPNTPVDRGDINQRSAIIVNALASFGINVVVKDVRFGPSVTQY
                     ALETEPGTKITKILSHQYDLALALASPTGSVRIEAPIPGKSLIGVEVPNNTRVVVHFK
                     ELLLSEQMKNMKSKIGMVLGLDVGGQPKVYNIAKMPHLLIAGATNSGKSVLIHNIMFS
                     ILFRASPEEVKFILIDPKRVELMYYNDIPHLLTPVITETEKAPAAFKWLVGEMTRRYN
                     LLESAHVRNIESYNEKSGFQAMPYIVMIVDELGEIMVTDPAAVEKSIIRLAQLARATG
                     IHLVLALQRPSANVVTGLIKANVPCRIAFNVTSQVDSRVIIDQPGAEKLLGKGDMLFI
                     PPDDSKPTRIQGSYIADNEINALVSYLKSTGVKPDYKEEILSTHEPEKSVSLGGDNVD
                     EYYDEAMRTVISEGRASASLLQRRLQIGYSRAARILDELEANGVIGPARGAKPREILN
                     NSIADLEDEEDVI"
     gene            11528..11815
                     /locus_tag="PLF29_00595"
     CDS             11528..11815
                     /locus_tag="PLF29_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52476.1"
                     /translation="MELQTFLIITLIFITLSIVAVSVYIILILREFKSTVQKVNGVLS
                     VTETLSTKLANTSITFGNIVNAVMGAVKTVKTIRSIADFGDEENKDAKSKR"
     gene            11796..12095
                     /locus_tag="PLF29_00600"
     CDS             11796..12095
                     /locus_tag="PLF29_00600"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HXK52477.1"
                     /translation="MPKAKGKFIKGLFLGAAIGTVLGLLNAPAKGSETKKSLKSKSEN
                     FKKKINALEQEDIEQLKKRVNKDVKSSISKSLKILASKIEKENEKSNKDKTDRIA"
     gene            12125..12934
                     /locus_tag="PLF29_00605"
     CDS             12125..12934
                     /locus_tag="PLF29_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52478.1"
                     /translation="MDLNAAQNTNNQQLNSHPDDAQKNKLNTILPGTNSQSLGGNSPI
                     TSIAGVSDDDLQNKSINLDTQDTLSTENKTNTNKEIEKAKGYKDDISKIVYDFLEKNG
                     ESLDSMEEVPSDIGPSINNHPATLGPGKGRREIFTSPVIGTISENAPISHRETFQTEI
                     DPASQTEQISVAKAETSAQEEKNSSEIQSIQEVLQNPQPDSENYDKISTLIPETEPIP
                     QPDSDLNVVDLRTGHENLQQIADPIDKLTEEADEKEADFITKVEDTHAVAN"
     gene            12918..15326
                     /locus_tag="PLF29_00610"
     CDS             12918..15326
                     /locus_tag="PLF29_00610"
                     /inference="COORDINATES: protein motif:HMM:NF024108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HXK52479.1"
                     /translation="MLSLTDIAYNIFWILFYIIFLASTILGIFYMLAKTKRQRFLKNK
                     QYNLTFFHVKVPQQNEFEIDAAEQLFAGVAGLRRSTLEGLLKGQYQVSFEILAKHGQI
                     GFYIVMPDELVEFMEKQINGAYPTAEIDIIDPNEVWDRGHFTAIAELKLKGPAFYPIR
                     TIKEKEGDPLSTLTSVLTKLKKDEVIAIQLVTSPASNWWRAAGNAFIGHVKHGGNPEK
                     KSAPDNSMLEGVEKKIGKSGLDVVVRVVSIAETKHIAESHIMNVVTAFEQYTDVKYNK
                     FKKSRVLFKTKFVNDFIYRNITPLNIYIPILYNTLYSRTSVLNITEMANIFHMPNKNI
                     QTPNILWLIARKASAPSNIPHEGLYLGINQFRGSQRGIYMKEKDRTRHMYIIGQTGTG
                     KSEFMKTLVLQDIAAGKGLALIDPHGSDIDDILEKIPESRIDDVILFDVSDIERPMGL
                     NMLEAETEEEKHMIINSFIALLYKLYDPNRQGIMGPQLERAIRNVMLTAMVDKESTMV
                     DVLRLLIDNKYVETFIPRIQDPLVKRYWTDEMANTSDYHKGEKMGYFVSKFDRFVTER
                     TMRNILGQPKSAFNFNKIMAEKKILLVDLSKGKIGEENSNFIGLLLVPRILAAALSRA
                     SLLGKEDFPDFYLYVDEFQNFATPDFATILSEARKYKLNLVVAHQFVAQLTDDIKEAV
                     FGNVGTTCVYRVGPDDAEYLEKQFDPYFTKSDLLNLPIGNAYMRLLVDGHPTTPFSLA
                     VDWDMVNKTKKDPSVAKRIRENSSMKYGTPVNEVEEYINKRAGLNKQGEDSQLLEKRS
                     LIPF"
     gene            15337..17214
                     /locus_tag="PLF29_00615"
     CDS             15337..17214
                     /locus_tag="PLF29_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52480.1"
                     /translation="MVETATAANDALTALKGRSETKVSPLEEFWTNINALTAASRQLL
                     TKIETSPQSGLNQRRAAQLARQALASYEMTTSLTENPTNREEINALSRLGAATNIDPT
                     NLYRETILLQPEVHSYEYSVKLLFSHIVSMTTEDRSNTTKNLALVLAGNCLDGSKVDV
                     RKIQNELRELNFPNHHLSEAAEAIAAIIRHTPYNLTDHQEKLGVTGYPEVLAKAYKRL
                     YDLATKLDPDTPPTKAEERSQHQPYANWEARAKERESIHYNSNPREHKIFSRKGEILT
                     AKGSENKTAQVLIPTAILTRTVPLADFAGNDRAFNKGYVKLQQVFTCTTFQEVEATLE
                     MLNAASIAEEEKTAQVPIYVPKTVVQNGKTTIEYEITYMEMPIGTYNSWRKALMEARK
                     SYLRAGFSIQNQDITAGVRLATEQENAYAEQTMLNRYQELLSGPSHIKDSLDAWMSQS
                     HSRDRFYIKELMQNTMELVNELTEELNLTRNPRNITLEAMKTLYEEGHEQHPLTMEKE
                     ITQMFDNRMAERSNYYVAMKVALTTLENVNALPPPRDRKEAKEQIRMIINVQTQIKQI
                     MLFLALQEKGVSESRASQVALENFPEHSVIKAQVPYRARNSQARRVAGKRQAVSNQGS
                     S"
     gene            17278..18483
                     /locus_tag="PLF29_00620"
     CDS             17278..18483
                     /locus_tag="PLF29_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52481.1"
                     /translation="MFKLPFKINFNKKSKTDTFLTIDIGSSTVKTLAFYNDSESRLKV
                     IGLGKEFLPEGAVRAGYITDLKSVIEAVDETIYQALANTEHRITDAIIGISENLCYSQ
                     VTTGRLNRTTPGELVTEKELDNLKYKIIDAAYNTALDDFVYKTGDLSTELIHISSIPA
                     HTKLDSKIVDDPTDLPGAKVELAIYNAYSPEYHTKQLQKIADTLKLSVLAMVPNMFSL
                     GEALKKLTKDNELDCIILDIGGETTDVGIMFGGGLITTKSLPIGGRHYTEQLSLQSGL
                     TWAESEKKKNQYYFGQTADTETSFIETVMTETTDLWLSGLETLFNEFNGVRTFASDIF
                     VVGGGSLLPDIMAALESRPWTKSIPFREPPRIKRVSISELDFARDTTGKLNELDYIQA
                     LSLGLMGEY"
     gene            18484..20013
                     /locus_tag="PLF29_00625"
     CDS             18484..20013
                     /locus_tag="PLF29_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52482.1"
                     /translation="MTKFEIEIHDDLINVLGKLQTINDDGIELIIPEGSVIFENILNL
                     RIIQKELEKSGKTIHFETSDENGKRLIENLENDSSGLSSELDFTEAEATEINPKTHRK
                     KQRTVSLALPKFKFSGGTKFKIAGLIGVVTAVIIGSLYLLIWKQHKAIVNLVVEAQPL
                     VKSVQIRVATNSETNTEQKTLKGSQVSTSTVQTASVPTTGEKLVGNQASGTVKIYNKT
                     TNGKTFKKGTVLVYKKDDKTLKYTLDEEVEVPERTEDILQDPPLITWGEKEVDVTAEE
                     IGNSYNIDSGESLDFDDFDKDDYTAKSTTKINGGSSETLKIVAQEDIDNLLIELEKSD
                     DKTKTELQQKTSTGRKLIPGSISLQTTKDSFSAELGDEAENLELVREATATGLTYSEN
                     ELEVLLDRLVDDFVPEGFELSTKDKEIKAEVLGATETTTLSSEQADLQVTIKAYVIPK
                     IDEIELRENLAGAPLSKAEEILNAISNVATYSLDVKPNIPLLNSLPHNPENIEITVTK
                     R"
     gene            20016..20453
                     /gene="ruvX"
                     /locus_tag="PLF29_00630"
     CDS             20016..20453
                     /gene="ruvX"
                     /locus_tag="PLF29_00630"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HXK52483.1"
                     /translation="MKNKNTPSKNTTYLGVDYGKKRTGLALGRNGLTAILTAIDSKDT
                     IYALNELAKIIALNKVDVIVFGLPLDAEGKDTAMSIEVRTFANSLKRKIKKNIQYVNE
                     FQSSVEATEDAISSGLSKKGRKSVDSISAGIILKRYFENLDTE"
BASE COUNT         7362 a         3920 c         4346 g         4888 t
ORIGIN      
        1 cggtgggcag ctgtgtgggc caaaagggtg taagagtaca ggctataatc tcagagctat
       61 tcggggaaaa aatagatata atcccctttt ctgctagtat tgaaaagtac atagcagcag
      121 cgctatcgcc agctagagtt acgggagtag aaataatcga agatgaaaat agggccgtag
      181 tatcggttcc ggaagatcag cagagccttg ctataggacg cgaaggacaa aacgcccgcc
      241 tagcaaataa actaactaaa tggaaaatag atataaaggg cacaaaaggg gtattcgaca
      301 caaccgaaga cacatcttca cagtcaactg aaaataggga tgtggtagga gtatgggacg
      361 ctgctattga aaaagtaaac aagcagttag aggactctgc caaaacaaca gaagaaaaaa
      421 tacaagaaaa cactgagagc gaaaaaacag aatctaccca atagtaagac ctaaacaaga
      481 agcattagag tgtgaggaaa aaaataaaag ggctttttaa atccttcccg tgctaaaata
      541 ttgcgttaga tttacacata attaaaagat cggtgtatag taaaaggtga acatgagtac
      601 aacaaataaa acaaatacta aatcaaataa agctgtgaga cctcctattg taacaataat
      661 gggacatgtg gaccacggta aaacgagcat actggattac attagaaaaa caaaaatcca
      721 agaacgagag tacggcggaa taactcagca cataggcgca taccaaataa gtcataaatc
      781 taaattgata acatttatcg atacaccggg gcacgcagcc ttttctcaaa tgcgttccag
      841 agggggcaaa gctgccgatt tggtggtttt agttgtagcc gctgaagaag gcgtaaagcc
      901 gcaaacaaaa gaagcgattc tacacgctaa agcggctggg gtaccaatta ttgttgcaat
      961 aaacaaaata gataaacccg gcgcggattc tcaaaaagta aaacaagagc ttgcccaagc
     1021 caatataatg gtcgaggatt ggggcggaga catagtatgt gtagaaacct cagcaatctc
     1081 cggaaaaggc atatctgagc ttttagacgc cattttagct gtagccgaga tggagaattt
     1141 agtagcagac agcaatgcgg aacttgaagc aataatcatt gaatcaaaac ttgaccctaa
     1201 aagaggtgtg gttgtaagtt gcatagttaa aagcggctcc ttgaaagtcg gctctgaagt
     1261 tacgggcagc ggcatagagg caaaagtaaa atcaatggta aatccttatg gtataagcat
     1321 tgatatcgca ggcccttcta cccctgttga aatactgggt tttaaaacca cgccgcacat
     1381 aggcgatttg attgtcgaaa aaggaagcga acttgctgag ttaagcattg ttgatgatca
     1441 caacgaagtt ataggggcta acacaaaacg tactgtagga attattatta gagccgacac
     1501 gcaaggcaca ctcgaagcag ttaaacagag cctagccaac ctcattaccg aatcggtggg
     1561 cttagacttt tctataaaat ttgtacacac tgcaacggga gatattacag actcagacgt
     1621 acttctggct tcaacaaccg acagcgttat cgtgggcttt aatgttaaag agggaaaatc
     1681 ggttactgag ctcgcatcag aactaaaagt aacagcaaaa acatataaaa ctatatacga
     1741 cctcatagat gaaataaagg atcttttaga aggtactgcg attaaagagg agcaaaaaat
     1801 aaaaggtcgt gctcaggtta taaaaaagtt taaacttcca tccggagacg taattttggg
     1861 cagtaaaatt ttggcaggaa aagtaaaaga aaacgacagg gtcggcatat ttctaatcaa
     1921 tccatcagag gccaccgagc aagatacgcc tctatattac tctaaaatta agaaaatcaa
     1981 ggttcaaaaa aacgaagtaa aagaaataaa aaaaggtctt gaatgcggcc tactgttaaa
     2041 acctcaaata gacgaaatcg agcccggcat gtacatagaa gttctttaga aactgcagtt
     2101 cgcaagcaaa taaggccaat acattgacac tataggacat ttattgtata ctctacgtgt
     2161 tccagcccat acagattaat atgcagatag aaaactacga caataatcaa atagccgagc
     2221 ttttgcgtga tgcaaaaagc gttggcataa tagcctcaag actaaatcaa ccggatactt
     2281 tctgtgccgc ttctggactc tacttgatgc tcaaaaatac gttcgaagac aaagaaatta
     2341 aacttattta caaaggcgac atccccacag aatgcagcaa tttaataaat agcgaagacg
     2401 ttcacgccaa tgtcgatgag agagacttgg tagtgactgt gaactacaaa gggacaagcg
     2461 catcaaaggt acaatatagc actgaagacg gagtatttca gttcaggctc ggccctgtgc
     2521 cgaaatattt tgatttgggc aacattcaaa gcgtactttc aggattcgac ttcgatgttg
     2581 tggttaccat aggtattcct gacctcgagg taacagggag tctatacagt agcctgaagc
     2641 acgaacttag cagcgcaaaa attgtaaaca tagacaatac aggactaaat aaaaggtttg
     2701 ggagcgtcaa tgtaattgat acgcaagcaa gctcacttag tacactggtc tttaatttag
     2761 cggcagaatg gaaactcaaa ccaactgcaa aatctgcaaa aatgctgtta gtaggggttt
     2821 catctacaag catagactag ttgaaataag tatttaatat tgttacaatg ttttagcata
     2881 tggcattaga taaaaagaat aaaacacaat taatacaagg tagcagactt cacgataccg
     2941 acacaggttc cccagaggtt caaataacta tattgtcaaa aagaatagcc gaactttctg
     3001 aacacctaaa aaaacacaaa aaagacaacc actcacgaag aggtcttttg caaatggtaa
     3061 ataaacgaag aaggctcctt gcatttttga agaaaaaaga tcctgaaaga ttcgagaaat
     3121 acgtagaaaa acacgagtaa tggcttttcc atatatcggg gtatagcgcc cgaaactttt
     3181 tcagtttttc catatttata gagtatcttg aggccaagcc tgggatccat gtgcttcaaa
     3241 gcacttaggc agatttgcca aatatacgca cagcgtataa agtatatagt tgaaagtata
     3301 aaaattaagg cggaagcttt gtgattaggg tcagacataa tgtacataat gttttaaata
     3361 ttatggatag tatctctcat cttagtcaca caacagcact ccgaataaaa taaatgaaac
     3421 aagtcaaaaa agaaatagac tttaacggta aaaaaattac gcttgaaaca ggcaaattag
     3481 cccctcaagc aaatgtatca atcaaggcca catacggcga aacggtcatc ttggtgaccg
     3541 cagtgtcagg cgggcagaat ccagacctgg attacttccc tcttcagatg tcttacgaag
     3601 agaaacttta tgcaagcgga acaataaaaa cttccagatt tgtaaaaaga gacggaagac
     3661 ccactgacga tgcaacagtc acacgaagac tcatagatag agctattaga cctctttttc
     3721 cggatgactt ttcggatgaa atacaaataa tcatgacagt attatccttg gacgaagatt
     3781 ccgatcctgc atttctagcg ggcttggcgg cttctgctgc gctgcaagcc tcaaatattc
     3841 catttgaagg ccccatgatt cctgtaaaag taggctatgc ggatcaaaaa tatattctca
     3901 taccgaccag aaaacaaacc gaagaagatt ccgatcttaa tatgatggtc tctttttcag
     3961 gaacagatag aaaattttta tcaattgatg ccagcgccaa tatcttgcca gaaaaagtaa
     4021 ttttaggagc aatagaattt gcaagagata acgcggaacc attgttaaac ctagttatgg
     4081 atttcgctaa ggaatataat ccagatggta cgaaatatac ttatatttca tcaaaacctt
     4141 cggatgaagt cattaactcc gtaaaagcaa ttgtaaaaga cgagctcgca acgttaataa
     4201 agggaaacta caagtacaac gaagctttaa ataatcaaaa gagcatgctt gtagaaaaag
     4261 tctttacaga acttgaaggc aagtttaaga aatccgaaat ggttaaagcg ctggagaaaa
     4321 tagagaaaaa catagctcga gctctcataa tggaatcagg aaaaagaatt gacggtcgtt
     4381 cagagagcga ccttagaccg atttcaggag aagtcgggct attgccacga acacacggca
     4441 gcggattatt taacagaggg ctgactcaag ctctaacagt tgctacatta ggaaatcctt
     4501 ctttagagct gcttattcaa gacatgtatg gagaaaaaac aaaacgatac ctccattact
     4561 ataacttccc tccttttgct acaggcgaag taggacgaat aggcggagcc aatccaagag
     4621 aaattggcca tgggatgtta gctgaaaaag cacttgtccc tgtaatacca agcaaagaag
     4681 agtttccata tacaatttta cttatgaccg aaactttatc ttcaaacggc tccagctcga
     4741 tggccgcgac atgtggctct accctggctc tgatggatgc tggcgtacca attaaaggcg
     4801 ctgtaggagg agttgcaatg ggacttatgg caaacgaaga tcaatcagaa tttatgattc
     4861 ttaacgatct tagcggctat gaagactctg ttggctacat ggactttaaa atgacaggaa
     4921 caaaagaagg aataaccgct atacaagtcg acatcaaact aaaaggctta ccaatggact
     4981 tgattccgag aataattgag aaatcgagag aagctcgact ggaaattatt gaaaaaatag
     5041 aagagattat ctcctcacca agagatagtt tgagtaaata cgcgccgaaa atgtgtgtag
     5101 tcaagattga tcctacaaaa atcggaatgg ttataggcag cggaggcaaa accatcaagg
     5161 aaattcagga gcagactaat actgagcttg gcatcgatga tgatgggaca gtatttatct
     5221 caggagaatc cgaagaagat gttgcacgcg ctcaaggaat ggttgagggc ctgaccaagg
     5281 atattgaggt tggagaagtg tacgaaggaa ctgtaaaaga aatagtagac tttggtgctt
     5341 tcgtagaaat tcttcctaat aaacaagggt tacttcatat aagcgagatt acagatgatt
     5401 acgtagaaaa ggcttcagat cacctaaaag aaggtcaagt ggttaccgta aaggtaatag
     5461 aaacaggccc taatggaaaa gtctctctat cgatgagagc catgaatccc gggtatgagc
     5521 ccaaaccaag gcacagcgac agaggaggaa gatcttcaag tggcggacac agcgggcgcg
     5581 atagaaactc acacaattcc ggaggcggaa gagatagagg cggaaggaac ttcagagata
     5641 gaagatagct tgaattttgt atgcggcaca ttaccaatgt gccgcatatc tgatagaatt
     5701 taaacatgcc cgacaaaaac tttagcattg cagacacccc caatctttca gccgcagact
     5761 acgcatttgc ggaactcaga acactttcgt cactggttaa tgacaccaga gatattcctg
     5821 aagaccttaa agataggctg aaccagatgc tcgatcgtct tgatagaatg gctaagcttg
     5881 gtcactatgc ccaagagttc gatacccttt ccagatatat cgagactatt gcagaaatac
     5941 cttggggaga aaaaacaaaa gacaatcttg atctaacaga aacaaaaaat attttggata
     6001 agaatcacta tggaatggag tgggtcaaag agagaatact tgaataccta gcaactataa
     6061 aacttttaaa agacagacaa aaagaagggg ttggaaaatc tccggttttg ttattagtgg
     6121 gcttacaggg cattggaaaa accacacttg caatgtcagt agctgaagcc ttgggcagaa
     6181 aatttattag aatatccatg ggaggtatag ggagtactct agaattaaga ggaagaagca
     6241 aagcctaccc tgaagctgag cccgggcaaa taattaaagc gttgatcaaa actggcgtta
     6301 aaaacccggt aatcttactt gatgaaattg aaaaagcaag cggagagtcg gggcttagat
     6361 cggatatcat ggcgatactt ctggaaatac ttgatccgag ccaaaataac agctttagag
     6421 accattatat tgactatccg atagatttat ccgacgtatt gttcatttgc tcagcaaaca
     6481 acaccggcac aataacaact gcacttatgg ataggttgga aataatcaaa atgccttcct
     6541 acacggatgc ggaaaaagct actatagcca aagactactt attacctaaa gttatcgaac
     6601 taagtggact aaagccggaa gaattagaaa ttgatccggc tctttggcct agcattgtgc
     6661 gtccttttgg ttttgatagt ggtattagaa gcctaagtag aactttagag gctatttgca
     6721 gaaaagtagc caaagaaata gttgaaggaa aaactaaaaa agtaagggta gacgaatcaa
     6781 atttaaaata ctaccttccc agctaacacg aacgggataa acaggtgtaa gcaagaaaaa
     6841 aatgagccaa aatacagaaa aactacaaat tatagaaaga gaaatacgca agtgcgaaaa
     6901 gtgttctctg tgcaaaactg ccctacaggc agtacccgga gaaggttcaa gcacggcaaa
     6961 agtagttttt atcggcgaag ctccgggagc aacagaagat aaaacaggcc gaccatttgt
     7021 aggaagagcc ggaaatctac tggaaaagct tcttgaaaaa ataaacctgt cgagggagga
     7081 agtttggata ggaaatatta tcaaacacag gccccctaag aaccgcgacc ctctgcctga
     7141 agaaattaaa gcttgcagag ggtatcttga aaaccaattg gcagtaataa agccatccct
     7201 gatagtgact ttggggagat tcgcaatgaa ccacttctat cccgaaggaa aaattacaaa
     7261 agatcacggc aaactaatca aactgcacaa tttcaatgtt tttcctgttt atcatcctgc
     7321 cgcggcttta agaaacagat catttgctga agctttagca aaagattttt taaatattcc
     7381 gaacgtactt acggaactta gtaaaaatga gaagagtaca aataacacaa tgatggcgaa
     7441 gccgttattg taaataacta attcacaata gaaaggattt ttagttaata atgaatacaa
     7501 ataattttaa tagcgtcaat tcaattcaac ctgaattacg aataataggt tttagcggcg
     7561 ccgaaggcgc cacaaaaaac atgacagtat atgagtccgg aaccgatatc ataatcgtag
     7621 actgtggtat aggctttccc gacgacgaac taagaggggt cgacgtagta atacctgatt
     7681 tcacgtatat tctggaaaat atcgagaaag taagagggct ctttgtaaca cacgggcacg
     7741 aggaccatct tggtgccata ccatatctcc tgaaagaagc gaatatcaag atcttcacaa
     7801 ccccgattgt tcaaggattt ataaggtcta ggcttttaga cagagggtct cagaaaatac
     7861 acgatgatgt gagtttttat acaatggtgc ccggaggaga agcagtaaca ttaggaaatt
     7921 tctctgtttc agcttttcat gttaatcatt ccgtacccga ttcagtaggg cttgcaataa
     7981 aaactccgca aggcacagta ttgcatatgg cggactataa aattgacctt gcaccggtaa
     8041 ttgataagcc catggatttt gcagcaatat caaacttcgg taaagaggga gtgctgtgcc
     8101 tgctttcgga ctgtctagga gccaataaag aaggccattc taaatacgag caatcgatga
     8161 agggcgtttt tacagatctc tacgaaaagg gcgaaggtaa gcaaataatc attactacga
     8221 tatcttcaaa tatatccaga atgtatcaga taatggacgc cgctactaaa tcagggagaa
     8281 aggttgtttt attaggcaga tcaataaata acagtgttga gattgcaaga ggtttagata
     8341 tgttgccttt ttcagatgaa ctttttgtca gccatagaga ctccatgaaa catagtcagt
     8401 cggagctggt ttacattgcg gccggatgtt tcggccagca aggttccgct ctcgatagaa
     8461 taagtagagg agaacacaga gatgttacgc ttgaagaaga tgcgttagtg attttctcgg
     8521 cagatcctgc acctccggga acaagaatac ctgtcgaaag attaatggac caactaacct
     8581 taaaaggtgc tgaggttata tacacaaaga ttcaggacaa tctgcacgtc tcggggcacg
     8641 gcccaaaggg agatctcgaa cttgtagcct caatggtaaa tccaaaatat ttcataccga
     8701 ttggcggaag cgtaactcaa atgagagctt acacgagcat ggtaaaaggt ttgggatatt
     8761 caacagatca ggtttttgaa atgcttgagg gggaaatatt ggaatttact gaaaatggag
     8821 ctttgatgtt ggacgagaaa atagacgtaa aacaggtcta tgttgacggc acagatgtag
     8881 gagaaatagg cgaagtagta ataaaagaca gagaaatcct cgcaaatgaa ggtgtctttg
     8941 tagttgtagt tccttactca aaaacagaca aacaggtgaa gggcaattct gacgtcataa
     9001 ccaggggctt tgtatatgtt aaaaactcaa aacaactcat ggggactgct aaagacttag
     9061 taaacaaaat tatcgataag tcatcaacgg acgagccttg gacaaagcta caagctaaaa
     9121 ttgaacaaga agtctcaaaa ctactgaaaa aagagaccgg aagagatccg atggtaatag
     9181 tccatgcatt atacgtgtaa ttttgttatt atctaagcaa tgtccgcaag aagaggccgt
     9241 aaaaaaaagt ttaagctgaa gtttaatata cgtggggaaa ctgtacgatc aattgcagcg
     9301 ttattactaa tattgctggc tggggtcgtg ctagtgtcct ttgctgcagg agtagtctca
     9361 gttgactcat ctattcaaag agttctaaaa attctatttg gatacgcgca ccttgctcta
     9421 gtgccgattt tccttatcac cggactactc ttaatagaca gtattgattg gagatttgta
     9481 gagggccggg tcttagccgg cttcagtata ctacttttcg gagcatcggg attacttcac
     9541 gcattctatc tcggagacgg ctacgaggca gcaaaagcag gaaaaggcgg gggaatagta
     9601 ggatacaaga ttttttctgt actaagttct aataccagct ctcttggggc aattcttgtt
     9661 ctgcttaccc tggttataat ctctatcctt cttatctggg ataagcctat cgaagaactt
     9721 gcagcaaaac tttctgcatt aggtctaccc ttctctaaac tcaaggcggc gggacttgaa
     9781 agaaaatcaa agaacgctag cacacaccag aaagaggata ttcacattac ttcaggactc
     9841 aatctaaact tcagcgataa cacaggttac aacgaaagca tagagagttc tccggaagaa
     9901 gatgcaacaa attatcacga cgaagattct gcagccgagg agatagaaat aattccttct
     9961 ctagccgagc ctaaatctaa gaattctcac gaattcaaaa caaatccaaa agtaacagat
    10021 actttggccc cggcacccct tccctattcg gatagggtgt gggaaaatcc ccccttggat
    10081 atcttaaacg atcctccgaa tactcccgta gatcgaggag acatcaatca gagatcggca
    10141 attattgtta acgccttagc ttctttcggc ataaatgttg tggttaaaga cgtgcgtttt
    10201 ggtccctctg taacccagta tgcgctagaa acggagccgg gaaccaaaat tactaaaatt
    10261 ttaagccacc aatacgactt agccctagct ttggcttcgc ccacaggttc ggttagaata
    10321 gaagctccaa tccccggaaa aagtttaatc ggtgtggaag taccaaataa cacgagggtc
    10381 gtagttcatt tcaaagaact cttactgtct gagcagatga aaaacatgaa atcaaaaatc
    10441 ggcatggttc tggggcttga tgttgggggg cagccgaaag tttataatat cgcaaaaatg
    10501 ccacacttac ttatcgcagg agctacaaac tcaggtaaat cggtactcat tcacaacata
    10561 atgttctcaa tactttttag ggcaagtccg gaagaagtaa agttcatact catagatccc
    10621 aagcgcgttg aactaatgta ctacaacgac ataccgcact tgctaacccc tgtaatcaca
    10681 gagacagaaa aagctcctgc agcttttaaa tggttggtgg gcgaaatgac caggagatac
    10741 aaccttctgg aatcggcgca cgtaagaaat atagaatctt acaacgaaaa gtcgggcttt
    10801 caagcaatgc catacatagt aatgatagtc gacgagctgg gggaaataat ggtaaccgat
    10861 cctgcagcag tagaaaaaag tattataaga ctagctcaat tagcaagggc taccggaata
    10921 cacttggtgt tagcgctgca aaggccttcc gcaaatgtag ttacaggact tattaaagcc
    10981 aacgtgcctt gtaggatcgc gtttaatgtt accagccagg tagattccag ggtaattatc
    11041 gaccagccgg gggcggaaaa attgctagga aaaggagaca tgctttttat tcctccagac
    11101 gactccaaac ccaccagaat tcaaggctct tatattgccg ataacgaaat caatgccctg
    11161 gtatcatatc ttaaaagcac aggcgtaaaa cccgactata aagaagagat tctctccaca
    11221 cacgagccgg aaaaaagcgt ctctctagga ggagataatg tcgatgaata ttatgacgaa
    11281 gctatgcgaa ctgtaatttc ggaaggcagg gcttccgcat ccttattgca gagacgactg
    11341 cagataggtt attcacgagc agcaagaatt ctggacgagc ttgaggcaaa cggtgttata
    11401 ggtccggcga gaggtgcaaa acccagagaa attctaaaca actccattgc cgatcttgaa
    11461 gacgaagaag atgtgatata attttttcat atactgcatg tatcagtaat ctaaaaataa
    11521 actgcctatg gaattacaaa catttttaat aattacctta atattcatca ctttaagtat
    11581 agttgcagtt tctgtttaca taatactaat acttagagag tttaaatcca cggtacaaaa
    11641 agttaatggt gtcctctctg ttaccgagac actatcgact aagctcgcta atacgagcat
    11701 aacctttgga aatattgtaa atgccgtaat gggtgctgtg aaaactgtca aaacaataag
    11761 atccatagct gattttggag atgaggagaa taaagatgcc aaaagcaaaa ggtaaattca
    11821 taaaaggttt gtttcttggt gctgctattg gaaccgtgct cgggcttctt aacgcaccgg
    11881 caaaaggctc tgaaacaaaa aaaagcctta aaagcaagtc agaaaacttt aaaaagaaaa
    11941 tcaatgcttt agaacaagaa gatattgagc agctcaaaaa aagagtaaat aaagatgtga
    12001 aaagctctat aagcaaaagt cttaaaatac tagcttcaaa aatagaaaaa gaaaacgaaa
    12061 aatctaacaa ggataaaaca gatcgtattg catagtactc ccggaataat gatacaatta
    12121 cagtatggac cttaatgctg ctcaaaacac caataatcaa cagctaaact cgcacccaga
    12181 cgatgctcag aaaaataagc tcaatacgat tttaccgggc accaattctc aaagcttggg
    12241 agggaattct ccgattacaa gcattgcagg ggtgtcggac gacgatcttc aaaacaaaag
    12301 cataaatcta gacacgcaag ataccctaag tacagaaaat aagaccaaca caaacaaaga
    12361 aatagaaaaa gcaaaaggct acaaagatga cataagtaaa attgtatatg actttctaga
    12421 aaaaaacgga gagagcctag acagtatgga agaggttcca tcagacatag gaccctcaat
    12481 aaataaccat cccgcaacac ttggccccgg aaaaggaaga agggagattt ttacaagccc
    12541 tgtaatagga actattagtg aaaatgcgcc tataagccat cgcgaaacct tccaaactga
    12601 aatagacccg gcttctcaaa cagagcagat ttctgtagct aaggcagaaa catccgctca
    12661 agaagaaaaa aattcttcgg aaattcaaag tatacaggaa gtattacaaa acccacaacc
    12721 agattccgag aattacgata aaatatcaac attgattcca gaaaccgaac ctataccgca
    12781 gccggatagt gatttgaacg ttgttgacct gagaacaggc catgaaaatt tgcaacaaat
    12841 tgccgatcca atagataaat taactgaaga agcagatgag aaagaggcag actttataac
    12901 aaaagtggag gatacacatg ctgtcgctaa ctgatatagc ctacaacatc ttctggatac
    12961 tcttttacat tattttcttg gcgagcacta tattaggaat attttatatg cttgccaaaa
    13021 ccaaaagaca aagatttcta aaaaacaaac aatataattt gaccttcttc catgttaaag
    13081 taccacaaca aaacgaattc gaaatagatg cggcagaaca actttttgca ggcgtggcag
    13141 gtttgagaag atcaactctg gaaggacttt tgaaaggcca ataccaagtc tcatttgaga
    13201 tcttggccaa acacgggcaa ataggcttct atatagtaat gcctgacgaa ttggtggaat
    13261 ttatggaaaa gcagattaac ggggcctatc ctactgccga aatagacatt attgatccaa
    13321 acgaagtttg ggacagaggg cacttcacag ctatcgcaga gctaaaactt aaaggaccgg
    13381 cattctaccc tattagaacg ataaaagaga aagaaggtga cccgctaagc actctcacct
    13441 cggtactaac aaagctgaaa aaagacgagg ttatagctat tcagcttgta acctcacctg
    13501 ccagtaactg gtggagggct gcaggaaatg cttttatcgg acatgtcaaa cacggcggaa
    13561 atcccgaaaa gaaaagtgcc ccagacaact ccatgctgga aggtgttgag aaaaaaatag
    13621 gaaaaagcgg gcttgatgtc gttgtgagag ttgtctcaat agctgaaacc aaacacatag
    13681 cggaaagcca cataatgaac gttgtaaccg ccttcgagca gtatactgat gttaaataca
    13741 ataaattcaa aaaaagcaga gttttattta aaacaaaatt tgtaaatgat ttcatttaca
    13801 gaaacataac gcctctaaat atatacatcc cgattttata taacacactt tatagcagaa
    13861 cctcggtact gaatattact gagatggcaa acatcttcca catgcctaac aaaaacatac
    13921 aaactccaaa cattctgtgg ctaattgcaa gaaaggcgtc agccccgtca aacatacctc
    13981 acgaaggcct gtatttagga atcaatcagt ttagaggctc acagagaggt atctatatga
    14041 aagagaaaga tcgtacacga catatgtata ttatagggca aacgggcaca ggtaaatccg
    14101 agtttatgaa gacactggta ctacaagata ttgccgcggg aaaaggtttg gctctaattg
    14161 atcctcacgg aagcgatata gacgatatct tagaaaaaat cccggaaagc agaatagacg
    14221 acgtaatact ttttgatgtt tcagatatag aacgcccgat gggattaaac atgcttgaag
    14281 ctgaaaccga ggaagagaaa cacatgatta ttaactcatt tattgccttg ttatataagc
    14341 tatacgaccc aaacagacaa ggtatcatgg gccctcaact tgaaagggct ataagaaacg
    14401 ttatgctaac cgcaatggta gataaagaat caaccatggt agatgttcta aggctattga
    14461 tcgacaacaa atatgtagaa acttttatac caagaattca agatccacta gttaaacggt
    14521 actggactga tgaaatggcc aacacaagcg actaccataa aggcgagaaa atgggctatt
    14581 ttgtatcaaa atttgatagg ttcgtaacag agaggaccat gagaaatata ctagggcaac
    14641 caaaaagtgc atttaatttt aacaagatta tggcagaaaa aaagatactg ctcgtagacc
    14701 tgtctaaagg taagattgga gaagagaatt caaactttat cggcctgctt ctggttccac
    14761 gaatacttgc tgcagctcta tccagagctt cccttttagg taaagaggat ttccctgatt
    14821 tttatcttta tgtagatgag tttcaaaact ttgccacacc tgactttgct actatactgt
    14881 cggaagccag aaaatataaa ctaaatttgg ttgtcgcgca ccaatttgtg gcacagttaa
    14941 ccgacgacat taaagaagcc gtgtttggta acgtaggaac aacttgtgtt tacagggttg
    15001 gtccggacga tgccgaatat cttgaaaaac agtttgaccc ttatttcaca aagtcagatt
    15061 tattaaactt gccaattgga aacgcttaca tgaggctgct tgtcgacggc cacccaacaa
    15121 cgcctttttc ccttgctgta gattgggaca tggttaataa aactaaaaaa gatccttctg
    15181 tggcaaagag aataagagaa aactcaagca tgaaatacgg aacaccagta aatgaggttg
    15241 aggaatacat aaataaaagg gcagggctga ataaacaagg cgaggatagc caactacttg
    15301 aaaaaagaag tttaatacct ttttaagaaa gacgctatgg tagaaacagc aaccgccgca
    15361 aatgacgctt taacagcatt aaagggaagg agcgaaacaa aagtttcgcc tcttgaagaa
    15421 ttttggacaa atataaatgc gcttactgcg gcttcgcgac aactgctgac taaaatagaa
    15481 accagccccc agagcggact gaatcaacgc agagccgcac agctagctag acaggctttg
    15541 gctagctatg agatgacgac tagcctgaca gaaaatccca ctaatagaga ggaaattaat
    15601 gccctttcca ggttaggcgc tgcgacaaac atcgacccaa caaatctata cagagagaca
    15661 atattactac agccggaagt acactcgtat gaatattcgg tcaagctgtt attctcccac
    15721 atagtgtcta tgaccactga ggacagaagc aacaccacaa aaaacttagc attggtttta
    15781 gccgggaatt gcttagacgg gagtaaggtt gatgtaagaa aaattcaaaa cgagctcaga
    15841 gaattaaatt ttccgaacca ccacttatca gaagcagcag aggccatcgc tgctataatc
    15901 cggcatacgc cttacaatct aacagatcat caagaaaaat tgggggtaac aggatacccg
    15961 gaagtcttgg ctaaagctta taagagactc tacgatctag caaccaaact tgatccggat
    16021 acaccgccta caaaagcgga agaaaggtca cagcaccaac cctatgccaa ttgggaagcc
    16081 agggccaaag aaagagaatc gatacactac aacagtaatc ctagagaaca caaaatattt
    16141 tcacgtaaag gagagatttt gactgccaag ggttcggaga ataaaacagc ccaagtctta
    16201 attccaactg ccatacttac cagaaccgta cctttggcgg actttgcagg caatgacagg
    16261 gctttcaata aaggatacgt aaaactacag caggtattta cctgcacaac atttcaagaa
    16321 gtcgaagcta cccttgaaat gctaaacgca gcatctattg cagaagaaga aaaaacagca
    16381 caggtaccca tatatgttcc taaaaccgtg gttcaaaatg gaaaaacaac aattgaatat
    16441 gagataacct acatggagat gccgattgga acctataata gctggcgtaa agctttaatg
    16501 gaggctcgca aatcctacct tagagcagga ttttctattc aaaaccaaga tataactgct
    16561 ggagtacgct tagcaacaga gcaagagaat gcctatgcag aacagacaat gttgaataga
    16621 tatcaagagc ttttgtctgg acctagccat atcaaagatt ctctagacgc atggatgagt
    16681 caaagtcatt caagggacag attttacatt aaagaattga tgcaaaatac aatggaacta
    16741 gtaaatgagt tgactgaaga gcttaatttg acccgaaacc ctcgcaatat aacattagag
    16801 gctatgaaaa ctctttatga agaagggcat gagcagcacc cactgacgat ggaaaaagaa
    16861 ataacacaga tgttcgacaa cagaatggcc gagcgctcta actattatgt tgcaatgaag
    16921 gtagctttga caactcttga aaatgtaaat gctttaccgc cgccaagaga tcgcaaagaa
    16981 gcaaaagaac agattagaat gattataaat gttcaaacac aaataaaaca aataatgctt
    17041 ttcttggcac ttcaagaaaa gggcgtctcc gagagccgtg cctcacaagt cgcgctggaa
    17101 aacttcccgg aacactcagt gattaaagct caagtacctt atagggctag aaatagtcaa
    17161 gccagaaggg ttgcaggaaa gagacaagct gttagcaacc aaggctctag ttgaaaaata
    17221 tcgagggcct gtttttcaaa tatactacaa aagcccattt atagtataat caagtaaatg
    17281 tttaaactgc catttaaaat aaatttcaat aaaaaatcaa aaacggatac ttttttaaca
    17341 atagacatag gatcaagcac agtcaaaaca ctagcttttt ataatgacag cgaaagcaga
    17401 ttaaaagtaa tcgggctggg gaaggaattt ttacccgagg gtgcggtacg agccggatat
    17461 ataactgatt taaaaagtgt catagaagca gtggacgaaa caatttatca ggcccttgct
    17521 aatacggaac acagaattac agatgcgata ataggaatat ccgaaaacct gtgctattcg
    17581 caagttacaa ccggccggct aaacagaact acgccgggag aactggttac agagaaagag
    17641 ctggataatt tgaaatacaa aataattgat gctgcgtaca acacggccct agatgatttt
    17701 gtctataaaa ccggcgatct ttcaacagag cttattcata taagctctat tccggcccat
    17761 acaaaacttg attcaaagat tgttgacgac ccaaccgact tgccgggagc aaaagttgag
    17821 ctggcaattt acaatgccta ttcccccgaa tatcatacaa agcaactgca aaaaattgct
    17881 gacacgctaa aactttcagt tttagctatg gttcccaaca tgttctcgtt aggggaagcc
    17941 ctaaaaaaac tcacaaagga caatgagttg gattgtatca ttttagatat cgggggagaa
    18001 acaacagatg taggaattat gtttggaggg ggccttataa ctacaaaaag cttacctata
    18061 ggaggaaggc actatacaga acagctatct ttacagagcg gccttacttg ggcagaatct
    18121 gaaaaaaaga agaatcagta ctatttcgga caaacagcag atacagaaac atctttcatc
    18181 gaaacagtaa tgactgaaac aaccgacctg tggctcagcg ggctggaaac cctgtttaat
    18241 gaatttaatg gggtcaggac ctttgcatct gatatatttg tagttggggg aggaagcttg
    18301 ctacccgata taatggcagc tctagagtcc aggccatgga caaaatccat accttttaga
    18361 gagccgccaa gaataaaacg ggtcagcatt tctgagctgg attttgctag agatacaacc
    18421 ggcaaactaa atgaattgga ttatattcaa gccctgtctt taggtttgat gggagaatat
    18481 taaatgacaa aatttgaaat tgaaatacac gacgatttaa taaatgtgct tggaaaacta
    18541 caaacaataa atgacgacgg aattgagctc attattcctg aaggttcagt aatttttgaa
    18601 aacattttaa atcttagaat tattcaaaaa gaacttgaaa aatctggaaa aacaatacat
    18661 ttcgagacgt cggatgaaaa cggaaaaaga cttatagaaa atcttgaaaa tgactcctcc
    18721 ggcctttctt ccgagctgga ttttacagaa gctgaagcaa ctgaaataaa cccaaaaaca
    18781 cacagaaaaa aacaacgcac ggtctcgctt gcattaccta aatttaaatt ttcagggggt
    18841 acaaaattta aaatagcagg tttaatagga gttgtgaccg ccgtaatcat aggaagttta
    18901 tacctattga tttggaaaca gcataaggca atcgtaaatt tagtcgtgga ggcacagcct
    18961 ctggttaaga gcgtacagat tagagtggca acaaacagcg aaacaaatac cgaacaaaaa
    19021 actctaaaag gctcacaagt aagtacaagc accgtccaga ccgcgagcgt gccaacaaca
    19081 ggggaaaaac tggtaggtaa ccaagcctca ggaactgtaa aaatttacaa caaaacaaca
    19141 aatggtaaaa cctttaaaaa aggtacggtt cttgtctata aaaaagacga taaaacccta
    19201 aaatatacac ttgacgaaga agtagaagta cctgaaagaa cggaagatat cttgcaggat
    19261 ccgccattaa taacttgggg tgaaaaagaa gttgatgtta cagcagagga aataggaaat
    19321 tcttataata ttgactcggg cgaaagtttg gattttgatg atttcgacaa ggacgattac
    19381 actgcaaaga gcacgacaaa aatcaacggg ggcagcagcg agacacttaa aattgtggcc
    19441 caagaagata tagacaacct acttatcgag ctggaaaaaa gtgacgataa aactaaaacc
    19501 gagctacagc aaaaaacttc gacaggacga aaattaattc ccgggtcaat cagccttcag
    19561 acaacaaaag attcctttag cgccgaacta ggagacgagg cggaaaatct agagcttgtc
    19621 agagaagcga cggccacagg gctaacttat tccgaaaatg aactggaagt tctactagac
    19681 agactcgtag atgatttcgt cccggaaggc tttgagctat cgacaaaaga caaggagata
    19741 aaagcagagg tcttaggtgc aacggaaact acgacacttt caagcgaaca ggccgattta
    19801 caggtaacca ttaaggctta cgtgatacca aaaattgatg agatcgagct aagggagaat
    19861 ttggcagggg ccccgttgtc gaaagcagaa gaaatattaa atgctatttc taatgttgca
    19921 acctattctt tagatgtaaa accaaatatt ccgctgctta attcccttcc ccataatccg
    19981 gaaaatatag agataacagt aactaaaagg taaaaatgaa aaataaaaat actccctcaa
    20041 aaaacactac ctatcttggg gtagattatg gaaaaaagag aaccggcctt gcgttaggaa
    20101 gaaacggact cactgcaata ctcacagcaa tagactcaaa agacacgatt tatgcattaa
    20161 atgagttggc aaagattatt gccctaaata aagtggatgt catagttttt ggacttccgc
    20221 tcgatgccga gggtaaagac actgcaatgt ctatagaagt tcgaacattt gcaaacagcc
    20281 taaaaagaaa aattaaaaag aatattcaat atgtaaacga attccagtca tcggtggaag
    20341 caacagaaga tgcaataagc tcgggtttat caaaaaaagg tcgaaaatct gttgacagta
    20401 tatctgccgg aattattttg aaaaggtatt tcgaaaatct tgatacagag taaaggcagg
    20461 gatcccgtag cgaatatgag ctacctcttt tcctccatta caacatcctt tgagcc
//
LOCUS       DAOSWN010000005        20284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_1662_length_20284_cov_4.950171, whole genome shotgun sequence.
ACCESSION   DAOSWN010000005 DAOSWN010000000
VERSION     DAOSWN010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 20284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20284
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1662_length_20284_cov_4.950171"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="PLF29_00635"
     CDS             complement(<1..694)
                     /locus_tag="PLF29_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52484.1"
                     /translation="MHKELTAKVQNITPKIPAETKVLSGAMIITPDDDLTLKRKGIIY
                     AVYSLNSNHQYEAGLTHKIVNDVLHDSYFQSDNASPVQAIEKAILKLRDNLLTLIDSS
                     ATSATQTDFSIATTVLWGNTLYLVTYGNAEIFLMRKGAIKPVNSAKEGKFSIASGVVK
                     DNDIIILATASFIAKYPPEKLIQVSSIPADDLGLDEASLMLKFIIKKELDEKDMLQIQ
                     SQEEDTASESLKN"
     gene            complement(705..959)
                     /locus_tag="PLF29_00640"
     CDS             complement(705..959)
                     /locus_tag="PLF29_00640"
                     /inference="COORDINATES: protein motif:HMM:NF040135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52485.1"
                     /translation="MFIDSASTANIISSFFTPEGFVNLLQMLVLFLEFVYLLIAFLIT
                     RQISLMNKSFKTEAALLFTIAGYINFLGTAALLILSVATL"
     gene            complement(949..1521)
                     /locus_tag="PLF29_00645"
     CDS             complement(949..1521)
                     /locus_tag="PLF29_00645"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HXK52486.1"
                     /translation="MLKTLYCSFLEKPKNMGFEGEDRDEHILYLFRKSFLTNAGWITA
                     TIIMLTVPFLANSVLISLNSKNAGLIPVQFILVLNIFWFLFTFGFALTSFVNWFFNIY
                     LISNKRMVDMDFVGILHKNISEAPLTSVEDTTSNVSGALRTVFNIGSVYIQTAAERRE
                     FEFIDVADPAKVRDLISDLVTEKKEAKDVH"
     gene            1666..2598
                     /locus_tag="PLF29_00650"
     CDS             1666..2598
                     /locus_tag="PLF29_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52487.1"
                     /translation="MHSNRSDPSSPEQGFSGGRKRLIEVGPGLDLVLVKPEKAGARYA
                     ADLSAEFWLIDDIHSRWSSGLHNLAVPPVPWISPIARYGVPKPPNKYAEGLAFLERCV
                     PIPPSLAVREVITLVAELLKGLAAVYAVCGVINTDIKPGNVMLGATGPRSRLPKRLVV
                     VDWGLGTVLRRDSGVGGMGADKFVPLSPGLGVGVNAFIAPPELLKYSAGGERSLEACP
                     ILTYQMAALLLCLIVPHATIKEALDNKHGHSAVDARGQERFFIGQDTVRVLGDGFPLP
                     FKDLLVQALGPDPANRLSSELFGKALLGFVGKVT"
     gene            complement(2643..3494)
                     /locus_tag="PLF29_00655"
     CDS             complement(2643..3494)
                     /locus_tag="PLF29_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HXK52488.1"
                     /translation="MYISIDLGGTNTRIATSLNLTDIKEVHKFKTEDNFLRQKYQIND
                     TIEQFVKSINKKVEGVAIGVPGTLDLKKRKFGKIMNFPPLSGRHFDDLIDERFVADQK
                     KIFAMNDAALAGLGEAYAGAGKAYETIAYITLSTSVGGARIAHKKIDNSQLYFEPGQH
                     IINPNGNSLIYCTEKGCFAAYASGTAFKQSFGISPEKCADPKIWSSYARHLSIGLYNI
                     LCFWAPEAIILGGGVSKMFKHFHKPLQEYLAEMQPFSMPDIKQAELLDDAGIIGGFQY
                     LKGCLNL"
     gene            complement(3501..4424)
                     /gene="gnd"
                     /locus_tag="PLF29_00660"
     CDS             complement(3501..4424)
                     /gene="gnd"
                     /locus_tag="PLF29_00660"
                     /inference="COORDINATES: protein motif:HMM:NF007161.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HXK52489.1"
                     /translation="MFRKTRIGYIGLGRMGYNMVLRLLEKKYEVVVYNKTSDKTLKAT
                     EAGALPSLSITEMVGRLKPPRTIWLMVPYTAVDEVLNELAPLLSKKDVVIDGGNSFYK
                     NTVKRYENLKERGIAYIDAGVSGGPSGARNGTSIMVGGDAKVVKKLERLFKDLSVRDG
                     YVYLGNSGAGHFVKMVHNGIEYGMMQAIAEGFDVLKHAPYTLQLREVMLAYKNGSVIS
                     SRLMDWLDEAFRKYGENLDAITGSASHSGEGKWTAETAKDMGLSTPALQEAIKVREKS
                     QSNPSYQGKLISAMRGEFGQHPVFQENNKLE"
     gene            complement(4426..5085)
                     /locus_tag="PLF29_00665"
     CDS             complement(4426..5085)
                     /locus_tag="PLF29_00665"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HXK52490.1"
                     /translation="MQLFIDSADPIEIENAYRLGLISGVTTNPSLAAKSSQDYPKDIE
                     KILRLIHGPVSIETVATTYEGMVAEGTALSRLNKHVVVKIPCVSEGFMAAKKLSSHNI
                     AVNMTLCFTLNQALLAANAGVLFVSPFLGRLNEIERNSGFELLKKIKQAYSNYNIKTK
                     ILAASIRQAEDIETAALIGADICTVPYNILIGLSAHPLTKKGLDKFLTDWKGSGLSLP
                     V"
     gene            5200..5952
                     /locus_tag="PLF29_00670"
     CDS             5200..5952
                     /locus_tag="PLF29_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52491.1"
                     /translation="MNKKFTAPLIVSSLFVLSLLALSPALSVSAQLVSYNSSNEKDDS
                     MYGGERKEKSYEGYLGEVGGKDNEMNEDAIKDRIQQEREDKMMLGEEDRDSKQENREE
                     KLSSMCEEATARVDRKLKAFDENPTAHMMKYKNMKERLDKMASVIESYGIDVTELRVM
                     IKTWDGMVLQYSNAYVDGMEALRMTKKYECGNSDGMFREYLTKAQEQFRVAREERARI
                     RAYYADELRPLLLKLRAQITKEIRQSSSMEEE"
     gene            5955..6263
                     /locus_tag="PLF29_00675"
     CDS             5955..6263
                     /locus_tag="PLF29_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52492.1"
                     /translation="MNLDKKGNALIILLAAVAVLAVAVLGFVIMKRTDVDDGSSMVVV
                     EDKADVMVEDTKPLAPLPQEEEGTPEGVKNEDLEGLDSLMKEIDADQNADDALQDLKL
                     "
     gene            6596..8875
                     /locus_tag="PLF29_00680"
     CDS             6596..8875
                     /locus_tag="PLF29_00680"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HXK52493.1"
                     /translation="MEKKYNHKDVEAKIYRMWEEKELFKAKVDPKKKPYCIILPPPNA
                     SGEMHTGNVLMIAIEDLLIRWKRLMGYEVLWLPGTDHAGFETQTTYERKLAKQGKSRL
                     DFDRQTLYNNIWDFVHENKSLIENQIRQMGASVDWSRYTFTLDPFSLKLVCDTFEKMH
                     ADGLIYRGDYVVNYSFKWGTTFSDAETSYKEVSDPLYYIRYRLSDNSPSPKGEQEIVI
                     ATVRPETIFIDTHIAVNPKDENHSHLIGCKALNPITGHEMEIIEGSFVDPAFGTGIVK
                     LTPAHDKDDFQVALAQDLPIINIIDLQGLITQGPQKGMTIKKARKSAVEQLKDLERIE
                     KIDESYKHMVPVDYRSGDYIENLVLPNWFVKVDGLRNAALESIKNGEMKIYPKWRQLT
                     YVRWLENMHDWAISRQVVWGIRVPVWYDSSKNPNISVTFLDSGGQAVSGTLSEILGSY
                     TLKEVLSGLQTLRAPKNSEYVISQESPGENYVPETDTFDTWFSSGQWPLITLKYPDSE
                     DFKYFYPTSVLETGWEIVTRWISRMVMFGIYLTGKPPFADVYLHGHVRAIDGRKMSKS
                     LGNHIIPSDYIEQYGADALRMGLILGTAMGRDFNFPQDKILAFRNFANKIWNMARFMG
                     VLEDNYNQEEGKELTIYSIDELKSLLQPKDIEILVGFDAMLKYVDSSLKKYRFADAAN
                     SIYQFMWKDLASDYLEDAKGRLDKDVALSVLRSVYLECLKVLHPFMPFVTEEIWQNLT
                     AEKERPLAVQSWPKPYKMS"
     gene            complement(8886..9728)
                     /locus_tag="PLF29_00685"
     CDS             complement(8886..9728)
                     /locus_tag="PLF29_00685"
                     /inference="COORDINATES: protein motif:HMM:NF013660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52494.1"
                     /translation="MNQAKMPATSNIYPIWQPQGNSTHIIAKHVSDMLGVKTSHTGTL
                     DPMAEGVIIVLADKERLKKYEHAKWTKKYTFQILFGIETDTGDALGLFDSQSAEVCEE
                     GPAFGSTTLESFIGKYIQEVPIYSAIKHKGRPLHQHAKLRTQPTTLPKRSGEILALKH
                     IRTELKNIEALQQNIEARISNVSGDFRQEKIIQNWQKFFSIRDGLKKTAIAEFQVEMT
                     KGLYVRSLAQDICRYLGTPGLTLSIVRTHNGKYTKNNSYTLSDLFGDKITNYNFKSST
                     NIKT"
     gene            9752..10417
                     /locus_tag="PLF29_00690"
     CDS             9752..10417
                     /locus_tag="PLF29_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52495.1"
                     /translation="MKVVKGNKIEEFTVEQSLTYIEGFSRVHLDIGAGDGRYVYKEAM
                     LSVAEEKKSNTLFIALEPVSKQLEEYSKKSKKAKLSNCLFVVGSLDNIPVELEHRINS
                     ISILFPWGSLLQNVVFPEELLIPVISNVARNGRLFLTIVFGYAPEAEPSEMRRLGLED
                     ISLNSLYEKIIPRYIKNGFKVECVRQNSREDLKKYESTWSKRLAFGQSRPVFTLDLLL
                     QLP"
     gene            10417..11817
                     /locus_tag="PLF29_00695"
     CDS             10417..11817
                     /locus_tag="PLF29_00695"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HXK52496.1"
                     /translation="MPLKKIKTNTLPKAPPLKSLIGPSFIILGLGLGSGEIILWPFLS
                     SKYGLGIIWGALVGITLQFFMNMEIERYALVRGESVFVGFARKMKLLPYWFLISTFLP
                     WIWPGIAASSAKLFGHLLGVEQTQYLAIAFLLLMGLILSFGSVLYKTVERMQKVLIAV
                     GVPSIFLLSIVLAQRSDFISLAKGMVGIGIGYSFLPIGIPIASFLAALAYAGAGGNLN
                     LAQSFYVKDKGYGMGRYAGKIRNLLSGSSEKIDLTGSTFIVDDTSLPNFKKWWRSVNI
                     EHLVVFWLTGSVTILLLALLSYVTTHSLGLRASDISFVIYESAEIGRRLFPFAGTFFL
                     IVAGATLFGTQLTVLDATSRILTENLSIIKDEKSGISSRFVTKTYYSVLWLQIGAGII
                     IFLLGFTEPLQLLITAAVLNAFAMFVHVGLTLWLNITLLEKELKPSALRVAAMIVAFL
                     FYGGFSAYTIINALLS"
     gene            12027..12103
                     /locus_tag="PLF29_00700"
     tRNA            12027..12103
                     /locus_tag="PLF29_00700"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12061..12063,aa:Pro,seq:ggg)
     gene            12128..12505
                     /locus_tag="PLF29_00705"
     CDS             12128..12505
                     /locus_tag="PLF29_00705"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXK52497.1"
                     /translation="MEASNITTELLVEDMKKTLDFYKDVLDFEVKMSFPEEDPFFAII
                     RNNKAELMLYTREKFLQDIPDFAKEKMGGTFALYIRVKDIVELFDDLKNKVKIIQELY
                     STSYVKLEFSFYDCNGYVLMLHE"
     gene            12623..13729
                     /locus_tag="PLF29_00710"
     CDS             12623..13729
                     /locus_tag="PLF29_00710"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HXK52498.1"
                     /translation="MRQFSLKFLLFIFVLLGSVASGMFAVGASYSGDKIYFFYGQGCP
                     HCEVVEEYFTDNNIYSKYQIDKKEIYGNKENARLLISVLSELGYPTDQIGVPTVVVGS
                     EVLVGDKIIIESFESAAENLKNSQNNLNKEIENIKKTENTAALPNLTVIAVAGAAIVD
                     AINPCAFAVLLILMTTVLARGSAKEALKSGLAFSLAIFVSYLLMGIGAYTALSYGNIG
                     KLLVRVIGIVAIVLGLFNIKDFFWYGKVFLIEVPLSWRPRMKKLINSVTSPVGALFTG
                     FIVSLFLLPCTSGPYIVILGLLANSSAKPQALAYLALYNIIFVSPMILITLAVYRGMD
                     PAKLETLRQKNLRILHLFAGIILLVLGFFTITSF"
     gene            13776..14246
                     /locus_tag="PLF29_00715"
     CDS             13776..14246
                     /locus_tag="PLF29_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52499.1"
                     /translation="MEHLAILRKSNIKKGDNLLGEILSGKKTIESRWYVHKSVPWGKV
                     KPGETIYFKESGYPVSARGKISNVLFFENLGPDLIKEIIIKYGHKIAPSFAQEALFEW
                     GRGLVKKRYCIIIFLTDVVPIKPFKINKKGFGMSSAWIVTPKIEELRLSAPARS"
     gene            14514..15233
                     /locus_tag="PLF29_00720"
     CDS             14514..15233
                     /locus_tag="PLF29_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52500.1"
                     /translation="MTSEVLKKELFTTRRFITIHEALNALIKVVSDAGCAQGFAVGFD
                     LDETLRQSFEDGLSGHPGRIRPEVYYLLKQLTRREVPIAVVTNQPSTGHQVSHVVAGL
                     RGYTLALPALRFLGIEVFGYESRPWSFLTFPASIATSVLGLGGLYKESPEAVSNFVSW
                     AETSCQGDLKHLIYVGNNNRDKDFTRLVALELAESGAGCAVEFYQLPNFSSFRSGDGR
                     SRSGGFLRGSRGGQAAQEIER"
     gene            complement(15294..15791)
                     /locus_tag="PLF29_00725"
     CDS             complement(15294..15791)
                     /locus_tag="PLF29_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52501.1"
                     /translation="MFGSVFRGRDAEVGSAVELLMGRPFSKTILGIENEMRKRKEAKS
                     DARNPKAGAEEEELFFLDALEVVIARTLAAHTENDGRTAAEILVIPPFASLYRLANIL
                     EQGHDFRVEITDDQNLLQKAYNEFLIAKHALEKREDPVKALNNRQATPGGAAVKINSL
                     RTWRN"
     gene            complement(15814..16686)
                     /locus_tag="PLF29_00730"
     CDS             complement(15814..16686)
                     /locus_tag="PLF29_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HXK52502.1"
                     /translation="MTKLKEVHAEFINKLKEDGKASSTIIAYNKDIEQLVESFTGLGV
                     NSPEKAELQHLVQFVSGLSDIGLTPKTISRKINATKTFFKYLTEKGHIPADVSEGLKH
                     PKIETKPPRILSKMEYRALRDTARKDVRTFTMIEVLLQTGISISEISLMKIDHINFDK
                     QELYVPARESKGGRTIPLNSAVIEALKTYIEKHRKTIKADAPHVFITKTGNKILVRNT
                     RSTIDRYFDLSGIIDARVNNLRHTFIAHHLSQGVSLQTVSKIVGHKRISTTEKYLDYI
                     EKETEKEKAELGVL"
     gene            16800..17216
                     /locus_tag="PLF29_00735"
     CDS             16800..17216
                     /locus_tag="PLF29_00735"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HXK52503.1"
                     /translation="MINKIKYIFTSILLLILSVGFLKSGLESSKGSSRSQNLENEVIG
                     LRDERDSLKKLLEYQKTDSYIEEEARNKLNMLRPGERVYVYTENVNSSEPGDLPLSQG
                     TDRGGSQRRVFRQNRAVDNELVGSKGNFEQWVDLLK"
     gene            17277..17350
                     /locus_tag="PLF29_00740"
     tRNA            17277..17350
                     /locus_tag="PLF29_00740"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17309..17311,aa:Met,seq:cat)
     gene            17529..17605
                     /locus_tag="PLF29_00745"
     tRNA            17529..17605
                     /locus_tag="PLF29_00745"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17563..17565,aa:Met,seq:cat)
     gene            17692..18723
                     /gene="trpS"
                     /locus_tag="PLF29_00750"
     CDS             17692..18723
                     /gene="trpS"
                     /locus_tag="PLF29_00750"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005769841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HXK52504.1"
                     /translation="MSGVFDTKKHKKRILTGDTPTGRLHLGHYIGTLENRVKLQHEYE
                     TFIILADTHAFAYPKYIDDPVYIAKATIDITIANLAVGLNPESTTFFVESGIPEIYEL
                     ATFFSMLVSHNRALRNPTIKDEIKMKSLGDSYSLGFIFFPLLQVADIIGVKADLVPVG
                     IDQKPHVEQALEVVSRFNSMYGEVFPVPKALIGRVGKLVGTDGNPKMGKSLNNCVFLN
                     DSEEDLKSKIMNMFTDPARIHASDPGRVEGNPVFIYHDAFNPNKDEILDLKDRYVKGQ
                     VGDVEVKTKLFNALNTFLGPIRDRYKYYDERREDVKDILIQGTARTREVVRETLEETK
                     DKMHLLNSL"
     gene            18821..>20284
                     /locus_tag="PLF29_00755"
     CDS             18821..>20284
                     /locus_tag="PLF29_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXK52505.1"
                     /translation="MLITKKKSQNSSSKGQKIQPGKNIKGRIKSGKSGPVKKQAPKDL
                     RSLLSNIYLYLFVFIIGVFILDGLGLIGPAPESIPFNEVLRLVNEDQVQDITVKDSDL
                     TILLKDGTTVTSKKEAGTSIFEVLSSESIPASKVAGSINIEQKFPLESVLSLLLSIGI
                     PLVILYIIFRSMRGASSDMFSFGRSKAKLFVKESSKITFNDVAGIDEAKSEMVEIVDF
                     LKNPKKYSQLGARIPRGVLLVGPAGVGKTLLAKAIAGEANVPFFSVAGSEFMEMLVGV
                     GSSRVRDLFAMAKESQPSLIFIDEIDAIGRQRGMGIGGGHDEREQTLNQILIEMDGFD
                     PRVNVIVLAATNRPDMLDPALIRAGRFDRRISLNLPDLKDREAILKIHAAGKPLNSDV
                     DFAKIAKKTVGFSGADLENMLNEAAILAARDSAVDITMNHIDEASLKVTMGPERRRLQ
                     DENEKRLTAYHEAGHAVVACHAGQLDSVERVTIVPRGG"
BASE COUNT         5630 a         3799 c         4510 g         6345 t
ORIGIN      
        1 cattttttag gctttcagag gccgtgtctt cttcttgaga ttgtatttgc agcatgtctt
       61 tttcatcaag ctcttttttt ataataaact tcagcatcaa gcttgcctca tccaaaccca
      121 aatcgtcggc cggaatacta gatacttgaa ttaatttttc aggcggatat ttagctataa
      181 atgaggcggt tgcgagtatg attatgtcgt tgtcttttac aacacctgaa gcgatagaga
      241 atttaccttc tttcgccgag tttacaggtt ttattgcgcc cttcctcatt aaaaatattt
      301 cagcattccc gtatgttacc aagtaaaggg tgttgcccca caaaacagta gttgcaatgc
      361 taaagtcggt ttgggttgcc gaagtggctg aagagtctat tagtgtaagt aaattgtcac
      421 gcaattttaa aatggccttc tcaattgctt gaacaggaga tgcgttgtca gattggaaat
      481 acgaatcgtg taatacatcg tttacgattt tatgcgttag gccggcttca tattggtgat
      541 ttgagtttag gctgtaaact gcatatataa taccttttcg ctttaaggtt aggtcgtcgt
      601 cgggggttat tatcattgct cccgacaaaa cttttgtttc cgcaggtatt tttggggtaa
      661 tattttgtac ttttgctgtt agctctttat gcatattttg ttatttacaa agttgccact
      721 gaaagaatga gaagtgccgc tgttccaaga aagtttatgt atccggcaat tgtaaaaagc
      781 agggcagcct ccgttttgaa actcttgttc attaagctta tttgtcttgt tattaagaag
      841 gcaataagta aataaacaaa ttctaaaaat aatactaaca tttgtagaag attaacaaaa
      901 ccttcaggtg taaaaaagct acttatgata ttcgcagttg aggcgctgtc aatgaacatc
      961 tttagcctcc tttttttcag taaccaagtc tgatataagg tctctaacct ttgcgggatc
     1021 tgctacgtct atgaactcaa attctcttcg ttctgctgcg gtttgaatat acactgagcc
     1081 tatgttaaat acagtacgca aagcacctga aacatttgag gtggtatctt cgacgcttgt
     1141 taaaggtgct tctgagatat ttttatgtaa aatgccaacg aaatccatat ctaccatcct
     1201 cttattgctt attaagtaga tattgaagaa ccagttgaca aagcttgtta gtgcaaaacc
     1261 aaatgtaaaa agaaaccaga aaatatttag tactaaaata aactgcacag gtattaatcc
     1321 ggcgtttttt gagttcaatg atattagcac gctgtttgcc aagaacggga ccgtaagcat
     1381 tattattgtc gcggtaatcc agcctgcgtt agttaaaaag ctttttctaa acaagtatag
     1441 tatgtgttcg tctcgatctt ctccctcgaa acccatgttt tttggttttt ctaagaacga
     1501 gcaatataaa gtttttagca tgggtacatt ataacatcat tcaaagaggt ggtggtcttt
     1561 ttcatgaatc aaatttatta cccccaaaag actttatcaa taatagtgtt tatttgttat
     1621 aatttagcaa tcccacccgt ttagttgaac agtaaataaa caagcatgca tagtaataga
     1681 tcagatccct caagcccaga acaggggttc tcaggaggcc gaaaacgttt aatagaagta
     1741 ggccctggtc tggacctagt attggttaag cccgaaaagg caggggcaag gtatgccgca
     1801 gatctaagcg ctgaattctg gctaatagac gacattcatt ccaggtggag tagcggcctg
     1861 cataatttgg cagtaccccc tgtcccatgg atctcaccca ttgcaagata cggagtccca
     1921 aaaccaccaa ataaatatgc tgagggactt gcttttcttg aaagatgcgt tcctattccg
     1981 ccgagccttg ctgtgcgcga agtaataact ctcgtagcag aattgttaaa aggccttgct
     2041 gctgtttatg ctgtttgcgg ggtaataaac acggatataa aaccgggtaa cgttatgtta
     2101 ggtgctacgg ggcctagaag tagattaccg aagcgccttg tggtagtaga ctggggtctg
     2161 gggacagtat tgaggcgcga ttccggggtt ggagggatgg gggcggacaa gtttgtacca
     2221 ctttctcctg ggcttggggt gggggttaac gctttcatag cccctccaga actattaaag
     2281 tatagtgcag gtggagaaag gtctttggaa gcctgcccca ttttaaccta ccaaatggca
     2341 gctttgttgc tctgtttaat tgttccgcat gcaactataa aagaagctct tgataataaa
     2401 catggtcaca gtgcagttga tgctcggggg caggagcgct tttttattgg tcaggatact
     2461 gtgagggttc ttggcgatgg ctttccgcta cctttcaaag atctcttggt gcaggccttg
     2521 ggccccgatc ctgctaatag gctttctagt gagttattcg gcaaggctct tttgggcttt
     2581 gttggaaagg taacataagc acaaagcagt agcaaatagg gctcttgctt aggagcatct
     2641 agctacaagt ttaaacaccc cttcagatat tgaaagccgc ctattatccc ggcatcgtct
     2701 aaaagctctg cttgtttgat gtcgggcatt gaaaaaggtt gcatttcggc taaatattct
     2761 tgaagaggtt tgtgaaagtg cttgaacatt ttagaaaccc cgcccccaag tattattgct
     2821 tcaggtgccc aaaaacacag aatgttgtag aggcctatgc ttagatgacg ggcatagcta
     2881 ctccagattt ttgggtcggc gcatttttct ggagaaatgc caaagctttg tttaaaggcc
     2941 gtgcccgaag cataggccgc aaagcagcct ttttcggtac agtaaatcag ggaatttccg
     3001 tttgggttta tgatatgctg gccgggctcg aaatacagtt gagaattgtc tatttttttg
     3061 tgtgcaattc ttgctcctcc aacgctagtg cttagtgtta tgtaggcaat tgtttcgtag
     3121 gctttgcctg ctcccgcgta ggcttcgcct aaacctgcta atgcagcatc attcatggcg
     3181 aatatttttt tctggtcagc aacaaacctt tcgtctataa ggtcgtcgaa atgccttccc
     3241 gacagcgggg ggaaattcat gattttgccg aattttcttt ttttaagatc cagcgttccg
     3301 ggtacaccaa ttgcaacacc ttcaaccttt ttgtttatag attttacgaa ttgctcgatc
     3361 gtgtcgttta tctgatattt ttgacgtaaa aaattatcct ctgttttgaa cttgtgcacc
     3421 tcttttatat cagttaggtt taaggaggtt gctattctag tatttgtgcc gccaagatcg
     3481 atagaaatat acatatataa ttattctaac ttattatttt cttgaaaaac agggtgctgg
     3541 ccaaactctc cacgcatggc tgaaattaat ttaccttgat agctaggatt agattggctc
     3601 ttttctctta ctttaattgc ttcctgcaaa gccggggtcg atagacccat gtctttggct
     3661 gtttcggctg tccatttacc ttctcctgaa tgtgaggcgg agccggttat tgcgtcgaga
     3721 ttttcgccgt attttctgaa agcctcgtcc agccaatcca taagcctaga tgaaattacc
     3781 gagccgtttt tataagccaa cattacctca cgcagttgta aggtataagg tgcgtgttta
     3841 agtacatcaa agccttcagc tatggcctgc atcatgccat actcaatgcc gttatgtacc
     3901 attttaacaa aatgcccagc tccggagttt cctaaatata cgtacccatc tcttacactt
     3961 aggtctttga atagcctttc gagttttttt acaacctttg cgtcgccacc aaccataatt
     4021 gaggttccgt ttctagctcc ggaggggccg ccgcttaccc ctgcgtcaat ataagcaatg
     4081 ccgcgttctt ttagattctc gtatcttttt acagtgtttt tataaaaaga attaccgcca
     4141 tctatcacaa catctttttt agaaagaagg ggtgccagct cgttcaggac ctcgtctacg
     4201 gcagtatagg gaaccattaa ccaaatggtt ctagggggct taagtctgcc taccatttcg
     4261 gtaatcgaaa gcgagggcag ggccccggcc tcggtggctt ttagagtttt atccgaggtt
     4321 ttattgtaga ctacaacttc gtattttttc tcaagtagtc taagcaccat gttatagccc
     4381 attctaccca gacctatata tcctatacgt gtctttctaa acatgttata cgggcagtga
     4441 gagcccagat cctttccaat ccgttaaaaa tttatcgagg cctttttttg ttaaagggtg
     4501 cgcacttagt cctataagta tgttgtatgg aacggtgcat atatcggccc ctataagggc
     4561 tgcggtttct atatcttctg cctgccgtat gcttgctgct aatatttttg tttttatgtt
     4621 gtagttagag taagcctgtt taattttctt cagtagctca aaaccggagt ttctttctat
     4681 ttcatttaat cgtccgagaa aaggagaaac gaataagacc ccggcgtttg ccgccagaag
     4741 agcttgattt aaggtaaaac agagggtcat gtttacggct atattgtgag agcttagctt
     4801 ttttgctgcc ataaaacctt ctgatacgca aggaattttt actactacgt gcttgttaag
     4861 tcttgagagt gctgttcctt cagcaaccat gccttcatag gtagttgcta ctgtctctat
     4921 gcttactggt ccgtgtatta gtctaagtat tttttcaata tctttagggt agtcttgcga
     4981 tgatttagcc gcaagagaag ggtttgttgt aactccggaa attagcccta atctataagc
     5041 attttctatt tctatagggt cggcagaatc aataaatagt tgcatatact aactatacaa
     5101 taagagactt ccaagatgaa cccatatgag ttgtattgaa tggcttgttt cgtgggttca
     5161 gattgacaac caggccacaa ttatttaaac tttatttata tgaataagaa atttactgct
     5221 cccctcatag tttctagttt atttgtttta tcactcttgg ctctatctcc ggcactttct
     5281 gtttcagctc aattggtgag ttacaattcc tcaaatgaaa aagatgacag catgtatggc
     5341 ggagagagaa aagaaaaatc ttatgaaggc tatctaggtg aggtaggcgg caaagacaac
     5401 gagatgaatg aagacgctat taaagacagg atacagcagg aaagagagga caaaatgatg
     5461 ctgggtgaag aagatagaga ctcaaagcag gagaacagag aagaaaaact ttccagtatg
     5521 tgcgaggaag caactgcgcg agttgataga aaacttaaag ctttcgacga aaatccaaca
     5581 gcacacatga tgaagtataa aaatatgaaa gagcgtcttg ataagatggc ctctgttata
     5641 gagtcttacg gtatagacgt tactgagctt agagttatga taaaaacttg ggacggcatg
     5701 gttctccaat acagcaatgc ttacgttgat ggaatggaag ctcttcgtat gaccaaaaaa
     5761 tatgagtgtg ggaattcaga cggcatgttc agagaatatt tgaccaaggc acaggagcag
     5821 tttagagtcg ctcgagaaga gagagcaaga ataagggctt attatgccga tgaactcagg
     5881 cctttgcttc ttaaacttag ggctcagata actaaggaaa taagacaatc aagttcaatg
     5941 gaggaagaat aagcatgaat ttagacaaaa aaggtaacgc attgataata ttattagctg
     6001 cggtagcagt acttgcagta gctgttttgg gctttgttat aatgaaacgc accgatgttg
     6061 acgatgggtc ctcaatggtt gtagtcgaag ataaagctga tgtcatggta gaggatacta
     6121 aaccactagc ccctcttccg caggaagaag aaggaactcc tgagggggtt aaaaacgaag
     6181 atcttgaagg tctagattct ttaatgaaag agattgacgc ggatcaaaat gctgacgacg
     6241 cgctacaaga ccttaagctt taattgactt gaggattttt atttggtaaa agtagaggcg
     6301 aaggccgagg ccttcgcctc tttcggttcc cttagtatag tgcgagctga aagttagaaa
     6361 caacataaaa agcgttataa gtttggatta atgtcaccaa acaagctgtg gaaagaaatc
     6421 atttagtagt aggcactggc ctttgcaaga cgaaaataga atccgccggg cacgcccgtt
     6481 atagctgtag caagtattat aaggctggta ccttccttta tggagcctct ggagaaatcc
     6541 ggaggttttt ttgtttgtga aaggcaggta agtatataat tagaaaacac gaaatatgga
     6601 aaaaaaatat aatcacaaag atgtagaagc aaaaatttat cggatgtggg aggaaaaaga
     6661 actttttaag gcaaaagttg atcctaaaaa gaaaccttac tgcataattt tacctccgcc
     6721 caacgcaagt ggagaaatgc ataccggtaa cgttctgatg atagcgatag aggatttgct
     6781 aattcgttgg aaaaggctta tgggctacga agtgctttgg ttgccgggaa ccgatcacgc
     6841 aggatttgag actcaaacta cttacgagcg taagttggca aaacagggca aatccaggct
     6901 ggattttgac aggcagactc tctacaacaa tatttgggac tttgtacacg aaaacaaatc
     6961 tcttattgag aatcaaatca ggcaaatggg ggcgagtgtt gactggagtc gatatacctt
     7021 tactttagat ccgttctcgt taaagttggt ttgtgacact tttgaaaaga tgcacgctga
     7081 tggcctcata tatcggggag actatgttgt taactactct tttaagtggg ggactacttt
     7141 ttcggacgcg gaaaccagtt ataaagaagt gtccgaccca ctgtactata taagatatag
     7201 gctatctgat aattcgcctt ctccaaaagg tgagcaggaa atcgtgattg ctactgtaag
     7261 acccgaaaca atctttattg acacccatat agccgttaat cccaaggatg aaaatcacag
     7321 ccatttaatc ggctgtaagg ctttaaaccc tataaccgga catgaaatgg aaattattga
     7381 gggttctttt gttgatcccg cttttggtac gggtattgtt aagcttactc ccgcacacga
     7441 taaagatgat tttcaggtcg cgctggctca agatctgcca attattaata tcatcgatct
     7501 tcagggatta ataacccagg ggccgcaaaa aggaatgact attaaaaagg cacgaaagtc
     7561 ggccgtagag cagcttaaag acttagaaag aatcgaaaaa atcgacgagt cgtataaaca
     7621 tatggttcct gtagattatc gaagtggaga ttatatagag aatctggttt tacccaactg
     7681 gtttgtaaag gttgatgggc tgcgaaatgc agctttagag tcgattaaaa atggtgaaat
     7741 gaagatttat ccaaaatggc gccagctcac ttatgtgcgc tggttagaaa atatgcacga
     7801 ttgggcaata tccaggcagg ttgtgtgggg cataagagtg cctgtatggt acgacagttc
     7861 caaaaacccc aatatttcag taactttttt ggattcaggc gggcaggctg tgtcggggac
     7921 tttgagtgaa attctagggt catacacttt gaaggaagta ttgtcgggtt tacaaaccct
     7981 aagggcccca aagaactctg agtatgtaat ttcccaagag agtccgggtg agaactatgt
     8041 tccggaaacc gatacgtttg atacctggtt tagtagtggt cagtggccgt taataacctt
     8101 aaaataccct gattccgagg atttcaaata tttttacccc acaagtgtcc ttgagacagg
     8161 ctgggaaatt gtgacgcgct ggatttctag aatggtaatg tttggtatat atttaaccgg
     8221 caaaccgcct tttgctgatg tgtatttaca tggacatgtt cgtgctatag atggtagaaa
     8281 aatgagcaaa agcttaggca accatataat accaagcgac tacattgaac aatacggagc
     8341 ggatgctttg agaatgggtt taattttagg taccgctatg gggcgtgatt ttaattttcc
     8401 tcaagataaa atactagcct ttagaaactt tgcaaataaa atttggaata tggcaagatt
     8461 catgggagtg ctcgaggaca actataatca ggaagaaggt aaagaactaa ccatttactc
     8521 aatagacgaa cttaaatcgc ttttacagcc taaagacata gagatacttg ttgggttcga
     8581 tgctatgttg aagtatgtag actccagtct caagaagtat agatttgcgg atgcggccaa
     8641 ctctatttat cagtttatgt ggaaagatct cgcttcggac tatttggaag acgctaaagg
     8701 aaggcttgac aaagatgtgg cgttaagtgt tttgcgaagc gtttatttag agtgcctgaa
     8761 agtcttacac ccttttatgc cttttgtaac agaagaaatc tggcaaaatc ttactgctga
     8821 gaaagagagg cctttagccg ttcagtcttg gccaaaacct tataaaatgt cctaaatttt
     8881 tttgattagg tttttatgtt tgtggaagat ttgaagttgt aatttgtaat tttgtcgccg
     8941 aacaggtcgc ttagagtgta agagttgttt ttggtgtact tgccgttgtg agttcttact
     9001 atcgacagtg ttaggcctgg agtccctaaa tatcgacaaa tatcttgcgc tagagacctg
     9061 acatataggc cttttgtcat ttctacctgg aattctgcga ttgcggtttt ctttaggccg
     9121 tctcttatag aaaaaaattt ttgccagttt tgtattattt tttcttgtct aaaatcgccc
     9181 gaaacgtttg agatccttgc ttctatgttt tgttgcagcg cttcaatatt tttaagctcg
     9241 gttctaatgt gttttaaggc gaggatctct cccgatcttt taggcagagt ggtaggttgt
     9301 gttcgaagtt ttgcgtgttg atgcaggggc ctgcctttat gcttaattgc gctgtatatt
     9361 ggcacttctt gtatatattt tcctataaaa ctctcaagcg tggtgctgcc aaaggcaggc
     9421 ccctcctcac aaacctccgc actctgagaa tcaaaaagcc ctaacgcgtc cccggtatct
     9481 gtttctatgc cgaaaagtat ttgaaacgtg tatttttttg tccacttggc atgctcgtat
     9541 ttttttagcc tctctttgtc ggctagtaca ataatcaccc cttctgccat tggatccagg
     9601 gttccggtat gagatgtttt aactccaagc atgtcagaga cgtgcttagc tattatatgg
     9661 gttgagttgc cttggggctg ccaaatagga tatatgttac ttgttgctgg catttttgcc
     9721 tgattcatgt gcttaatcta taatcttttt agtgaaagtt gtcaaaggaa ataaaataga
     9781 agaatttacc gtcgaacaat ccctgactta catagaaggt ttttcgcgtg tgcatttgga
     9841 tattggagcc ggggatggaa ggtatgttta taaagaggct atgttatcgg tagccgaaga
     9901 aaaaaagagc aacactttgt ttattgctct tgagcctgtt tcaaaacagc ttgaggagta
     9961 ttcgaaaaaa tccaaaaaag ccaagctaag caactgcctt tttgttgtag gttctttaga
    10021 taacattccg gtagaactcg aacacaggat taactcaata tctatactat tcccgtgggg
    10081 aagccttctt caaaatgtgg tttttccaga ggaattgttg attcctgtta tttcaaatgt
    10141 tgcccgaaat ggtcgattgt tccttacgat tgtttttggg tatgcccctg aggccgaacc
    10201 ttcagaaatg cgtcgtttag gccttgaaga tatcagttta aactctttat acgaaaaaat
    10261 tattccgaga tatattaaaa acggttttaa ggttgaatgt gtaaggcaga actctagaga
    10321 agatctaaag aaatacgagt ctacatggag taaaagactg gcttttggcc aaagtcgtcc
    10381 tgtttttaca cttgatcttt tgttacaatt accataatgc ctttgaaaaa aataaaaact
    10441 aatacactgc caaaagcccc tcctctaaag tcattgatag gccctagctt tataattctt
    10501 ggtttaggtt tgggcagtgg ggaaattata ctctggcctt ttttaagctc gaaatacggc
    10561 cttgggatta tttggggcgc tttggttgga attactcttc aattttttat gaatatggag
    10621 attgaacgtt atgctcttgt tcgcggggag agcgtttttg ttggctttgc tcgaaaaatg
    10681 aagctcctgc cttattggtt tttgatttcc acctttttgc cttggatttg gccgggaata
    10741 gccgcgtctt ctgcaaagct ttttggtcat ttgcttggtg tggagcaaac tcagtatttg
    10801 gcaatagctt ttttgctttt aatggggcta atattgtctt ttggttctgt gctttataaa
    10861 actgttgaga gaatgcagaa agttcttatt gcggtcgggg tgccttctat atttcttttg
    10921 tctattgttc tggctcagag gtccgatttt ataagcctag caaagggcat ggtcggaata
    10981 ggcattgggt attctttttt acctattggg atacctatag catctttttt ggccgcattg
    11041 gcttatgcag gtgctggagg gaacttgaat cttgcgcaat ctttttatgt taaggataaa
    11101 ggctatggta tggggcgcta tgccggcaaa ataagaaatc tactctcagg atcaagcgag
    11161 aaaatcgatc taacgggctc tacttttatt gtagatgata caagtctgcc taattttaaa
    11221 aaatggtggc gttctgtaaa tattgagcac cttgttgttt tttggctaac aggatcggtc
    11281 acaatacttt tattggccct tttgtcctat gtgacaaccc attctctagg ccttcgggcg
    11341 agcgacatta gctttgtaat ttatgagagt gctgagatag gtagaaggtt gttcccgttt
    11401 gcaggtacgt tctttttaat agttgcaggg gctacattat ttggaaccca gttgacggtt
    11461 ttggacgcta caagcaggat tttgactgaa aatctatcaa ttattaaaga cgagaagtct
    11521 ggtatttcaa gccgttttgt aacaaaaact tattactcgg tgttgtggtt gcaaataggc
    11581 gcgggtatta taattttctt gttaggcttc actgagccgt tacagcttct tattacggcg
    11641 gcagttctaa acgcttttgc gatgtttgtg catgtaggtt taactttatg gttaaatatt
    11701 actttgcttg aaaaggagct aaaaccctct gctttaagag ttgctgcaat gatcgtagct
    11761 tttttgttct acggcggttt tagtgcttac accattataa atgcactttt gtcttagttt
    11821 tttgcctatt tttgcttaag tagggctttt agtgctataa ctctctgaaa ggtttataat
    11881 ttcgttactt aaaactattt gcgttagcta gtagatgcag tttacccacc atgttgggct
    11941 ttgcaactta aagaaattat agcaaatcac ctgcgtaagc tgatggtggt tgattccata
    12001 aagtggtaaa atgtacgtac atttgtcggg gtatagcgca gcttggtagc gcgttcgcat
    12061 ggggtgcgag aggccgcagg ttcgaatcct gctaccccga ccattacccc tttgttataa
    12121 tagttctgtg gaagcatcaa atatcacaac cgagctcttg gtagaggaca tgaaaaaaac
    12181 gctagatttc tataaagatg ttcttgattt cgaagtcaaa atgtcttttc ctgaagaaga
    12241 tccgtttttc gctattatta gaaacaacaa agcagagtta atgctttata ctagagaaaa
    12301 attcttgcag gatatacccg acttcgcaaa agaaaaaatg ggtggaactt ttgctctata
    12361 catacgcgtc aaagatatag tcgaactatt tgatgacctt aaaaacaaag taaaaatcat
    12421 ccaagagctc tacagcacaa gctatgtcaa gctagaattt tctttttacg actgcaatgg
    12481 gtacgtactt atgttgcatg agtagtgttt tcaaaaaact ccccttttac cgcatttcga
    12541 cctgggcatt atagcctaat ctttctcttt atttcagggc caatatttcc taaacctctt
    12601 ttacggtcta taattagacc acatgaggca attttcacta aaatttctgc ttttcatttt
    12661 tgtgctctta ggatcagtcg cttcgggcat gtttgctgtt ggggctagct atagtgggga
    12721 taaaatctat tttttctacg gtcaggggtg tcctcattgc gaagttgttg aagaatactt
    12781 tacggacaac aacatttatt caaaatacca aatagacaaa aaggaaattt atggaaataa
    12841 agaaaatgct cgtttgttaa tctcagttct aagcgagctc gggtatccta ctgatcagat
    12901 aggtgtgcct actgtggttg taggttctga ggtgctggta ggagacaaga ttataatcga
    12961 gagttttgaa agtgctgctg agaacttaaa aaatagccaa aataacttaa ataaagaaat
    13021 cgagaatata aaaaagactg aaaatacagc tgctctccct aatcttactg ttatagctgt
    13081 cgcaggtgcc gccattgttg acgccataaa tccttgtgcc tttgcagtct tgctcatact
    13141 gatgacgacg gtattagcga gaggatctgc aaaagaagcc ttaaaatcgg gcctagcttt
    13201 ctctttagcc attttcgtgt cttacctttt gatggggatt ggagcttaca ctgctctaag
    13261 ttatggcaac attgggaagc ttctggttag ggtaattggt atagtcgcca ttgtcttggg
    13321 tttatttaac attaaagact ttttttggta cggaaaggtg tttttaattg aggttccttt
    13381 gtcttggaga cctcgaatga aaaaactcat aaattctgta acaagcccgg tgggcgccct
    13441 ttttaccggt tttatagtta gtcttttttt gctgccttgc actagcggcc cgtacattgt
    13501 aattttaggt ttattggcta attcttcggc taagccgcag gccttggcct acctggcttt
    13561 atacaacata atttttgttt cgcctatgat tcttataacc ttggctgtct atagaggtat
    13621 ggatcctgcc aaactagaga cgttacggca gaaaaattta cgaattctgc atctttttgc
    13681 gggtataata ctgcttgttt taggattctt tacaataacc tcattttgac ggatgacttt
    13741 tggatctggc aatattaaat tataataacg atattgtgga acatttagcg atccttcgta
    13801 agtccaatat taaaaaaggc gataatctcc tgggcgaaat tttatcagga aaaaaaacaa
    13861 tcgaatctcg atggtatgtt cataaatcag tgccttgggg aaaagttaaa cccggagaga
    13921 caatttattt taaggaatct ggatatccgg tttcggctcg aggtaaaata agcaacgttt
    13981 tgttttttga aaatcttgga cccgatttaa taaaggaaat cataattaaa tacggccata
    14041 agattgcgcc gagttttgcc caagaggctt tgtttgaatg gggaagaggt ttagtaaaga
    14101 aacgttactg tattataatt tttttgaccg atgttgttcc gataaagcct tttaaaatta
    14161 ataaaaaagg ctttggaatg tcatctgctt ggattgtcac ccctaagatt gaggaattaa
    14221 ggttgtctgc tcccgcgcgg tcttaaacat agataccgtc cctcccccta ccactatggc
    14281 tataggttgg gggtagcttg ggtcccttga cacctcctcc atctattata taatctagga
    14341 aagattagtt agccccgttt tttttatgca caaaatacta tagggcccga ttaattgtta
    14401 gatatttctt ttatataata tagtaattat tcctgctgct aagtataaaa attaaggcgg
    14461 ctttaaaatc tataaagccc ttaccatttt taactgctcc acttaggtat tttatgacaa
    14521 gtgaggtcct aaaaaaagaa ctttttacga cgagacgttt tatcacgatt catgaggctc
    14581 ttaatgctct aataaaggtt gtttcggatg cgggctgcgc gcaaggtttt gctgtgggat
    14641 ttgatttgga tgaaactcta aggcaatctt ttgaggatgg cctatcaggg catccaggcc
    14701 gtatacggcc cgaggtctat tatcttctca agcaacttac gcgccgtgaa gttcctatag
    14761 ctgtagtaac taatcaacct tctaccggtc accaagtgtc gcacgttgta gcgggactaa
    14821 gaggttacac actagctctt ccggccctta ggtttttggg cattgaggtt ttcggttacg
    14881 aaagtaggcc ttggagcttt ctaacattcc cggcctctat tgctactagt gtgcttggtt
    14941 tgggaggact ttataaagag tctcccgagg ctgtttctaa ttttgtttct tgggcagaaa
    15001 catcatgcca aggagactta aagcacctta tatatgttgg taacaacaat agagataaag
    15061 attttacaag actggtcgct ttggagttag cagagagtgg ggcaggctgt gctgttgaat
    15121 tttatcaatt gcccaacttt tcatcgtttc ggtcaggtga cgggagaagt aggtctgggg
    15181 gattcttacg cggatctcgg ggcgggcaag cagcccagga aatagaaaga taaaaccacc
    15241 ccccccctta taaaaaatag tttctaaagt tggggtttta tagtcgagcc ggattagttt
    15301 cgccacgttc tcaaagagtt gattttaaca gcagcccctc ccggagttgc ttgtctgtta
    15361 tttaatgctt tcacgggatc ttctcttttt tctagagcat gtttagctat taggaattcg
    15421 ttataagcct tttggaggag attctggtca tctgtgattt ctaccctaaa atcatggccc
    15481 tgctctagaa tattcgcaag cctatatagg gaggcaaagg gaggaattac cagtatctct
    15541 gcggctgtgc gtccatcgtt ttcggtatga gctgccagcg tacgtgcaat aacaacttct
    15601 aacgcatcta gaaaaaagag ttcctcttcc tccgcaccag ctttaggatt tcttgcgtcg
    15661 ctttttgctt ccttgcgttt ccgcatttca ttttcaatac ccaagattgt tttcgaaaag
    15721 ggcctgccca tcaaaagttc tacagcacta cccacctcag catccctacc acgaaaaaca
    15781 cttccaaaca tactgcacgc cctcccttat ttactacagt acacctaatt cagctttttc
    15841 tttctctgtt tctttttcta tgtagtcaag atatttttca gttgttgata ttcttttatg
    15901 tcctactatt ttagagactg tttgtaggct aacgccttga gaaagatggt gggcaataaa
    15961 ggtgtgcctc aaattattta ctcgtgcatc tataatacca gacaaatcaa aataacggtc
    16021 aatagttgag cgagtatttc ttacaagtat tttgttgcct gtttttgtta taaatacatg
    16081 cggggcatcg gctttgattg ttttcctatg tttttcgatg taggttttta aagcttctat
    16141 gacagctgaa ttaagcggta tggttcggcc gcccttgctc tctcgagccg gcacgtatag
    16201 ttcttgttta tcaaaattga tgtggtctat tttcatgagc gaaatttcag atatcgatat
    16261 tcctgtttgt aatagaactt ctatcattgt aaatgttcgc acgtctttac gtgctgtatc
    16321 cctaagagcc ctgtactcca tttttgagag aattctcggc ggtttagttt caatttttgg
    16381 gtgttttagg ccttctgaaa catctgcggg tatgtggcct ttctctgtta ggtacttaaa
    16441 gaaagttttg gtagcattta tttttcgtga gatggtttta ggtgtaagtc ctatatcgga
    16501 taggccggat acaaactgca caagatgctg tagttcggct ttttccggag agtttacgcc
    16561 caggcccgta aaactttcta ctaactgctc aatgtctttg ttataggcga ttatagtgga
    16621 tgaggctttg ccgtcttctt tgagtttatt tatgaattct gcatgcacct cttttagttt
    16681 agtcattttt tattcgtctt ctgagcagca atttcataat acctataggg cattttacat
    16741 acaactgtgc ataatgtcaa ttgtagcttt tttggcattt aggtataata cctttgccca
    16801 tgataaataa gattaagtac atttttacct cgatcctact gttaatactg tcggtaggct
    16861 ttcttaaatc cggcctagag tcttcaaaag gctcttccag gtcccaaaat cttgaaaatg
    16921 aggttatagg cctgagggac gaaagagact ctctgaagaa actattagag taccagaaaa
    16981 cggactccta cattgaggag gaggcccgta acaaattaaa catgttacgc cccggagaga
    17041 gggtttatgt gtacacggag aatgtaaatt cttcggagcc gggagatctt ccgctatcac
    17101 aaggtacaga tagaggagga tcgcaacgtc gggtttttcg ccagaatagg gccgtcgata
    17161 acgagcttgt tggttcaaaa ggtaattttg aacaatgggt ggatctttta aaataattct
    17221 atttgcattg tctttttgac attagtttgt gtgtttgata atatactccc gtacgtagcg
    17281 gggtggagga atagtacctc gtcaggctca taatctgaag gtcccggtgc aaatccgggc
    17341 cccgccacca aagtacactc ttagaccttt tatgcccgac ggctctatag tcccacagtc
    17401 cgacttttaa aaatattcct gatttgctat actcattgac gttgcccacg ctcatgctta
    17461 atttgtttgc acgggacttt atttaaggcc ttaccgcagc atagaggtga gcacggtaaa
    17521 atagtttcgc caacgtagct cagtcggtta gagcgggcgt ttcatacgcg ccaggtcgct
    17581 ggttcgattc cagccgttgg caccaacttt ttatgtcgcg agcatttttc gttctttccc
    17641 attccatttc tgtgttggcc gtaagaaaga tttttgctgt ataataacct tatgtcagga
    17701 gtttttgata cgaagaagca caagaaaaga attttaaccg gagatacgcc gaccgggagg
    17761 cttcatctcg ggcattatat aggcacttta gagaaccgag ttaaattaca gcacgaatat
    17821 gagactttta tcattttggc tgatactcat gcgtttgcat acccaaaata tatagacgat
    17881 cctgtttata tcgctaaagc tacgatcgac atcactattg ctaaccttgc tgttggttta
    17941 aatcccgaaa gcacaacctt ttttgttgaa tcggggatac ccgagatata cgagctcgca
    18001 acttttttct cgatgctagt gagccataac agggccttgc gtaacccgac aataaaggac
    18061 gaaatcaaga tgaaaagtct tggcgacagt tattctcttg gctttatttt tttcccactg
    18121 cttcaggtgg cagacataat aggtgtaaaa gccgatttgg ttcccgtggg cattgaccag
    18181 aaaccccacg tagagcaggc gctcgaagtt gttagcagat ttaactcgat gtatggagaa
    18241 gttttcccgg tgcccaaggc attaatcggt agggtcggca agcttgttgg tactgatggt
    18301 aatcctaaga tgggaaaatc tcttaataat tgtgtatttt taaacgattc cgaagaagat
    18361 ttgaagagta aaataatgaa catgttcact gaccctgcac gcattcatgc aagcgatccg
    18421 ggaagggtag aaggaaatcc agtttttatt taccacgatg cttttaaccc caataaagac
    18481 gaaattctag atcttaaaga tagatatgta aaaggtcagg ttggtgacgt tgaggtgaaa
    18541 actaaacttt ttaatgcact taacactttt ttggggccaa ttcgcgacag gtataaatat
    18601 tacgatgagc ggagagaaga tgttaaggat atattgattc aaggaactgc acgaaccaga
    18661 gaggtagtta gagagacttt agaagagact aaagataaaa tgcatctgct aaattcgctg
    18721 tgatataatc acctagctgc ttatttaaac cagtattatt gggagaccca aaataatttt
    18781 atagtagata taagactggt ttagagtaga tcgaaaaact atgttaataa caaaaaagaa
    18841 aagccaaaat agctccagca aggggcagaa aattcaacca ggaaagaaca ttaaagggcg
    18901 gatcaaaagc ggcaaatccg ggcctgtaaa aaagcaggcc ccgaaagacc taagaagcct
    18961 tctaagtaac atttatttgt atctttttgt ctttattatt ggggttttta ttttagacgg
    19021 acttggttta attggccctg ccccggaatc gatacctttt aacgaagtgc tgcgccttgt
    19081 taatgaagat caagttcaag atattacggt aaaagacagc gacctaacaa tattgttaaa
    19141 ggacggtacc acagtaacct caaagaaaga agcaggaact agcattttcg aggtattaag
    19201 ctctgagagc attcctgctt caaaagtcgc tggctcgatc aatattgagc agaagtttcc
    19261 cttggaaagc gtgctatctt tgcttttatc tataggaatc ccgttggtta ttttgtatat
    19321 catatttaga tctatgcgcg gggcaagtag cgacatgttt tcgtttggaa ggtccaaggc
    19381 taagcttttt gttaaagaaa gctcaaagat aacctttaat gatgttgcag gcatagacga
    19441 ggcaaaaagc gaaatggtcg agattgtaga ttttttgaaa aatccaaaaa aatactccca
    19501 gcttggtgcc aggattccca gaggagtttt gttagtaggt cctgccggag taggaaagac
    19561 tttgctcgca aaggctatag ccggagaagc caacgtgcca tttttcagtg ttgcgggaag
    19621 cgaatttatg gaaatgttgg ttggggtagg ttcgtcaagg gttagagatc tatttgccat
    19681 ggcaaaggaa tctcaaccca gccttatttt tattgacgaa attgatgcaa taggtcgtca
    19741 gcgcggcatg ggcattggag gcgggcacga tgaaagagag cagacgttaa accagatatt
    19801 aatagagatg gacgggtttg atccgagagt taatgtgata gttttagctg cgacaaacag
    19861 accggatatg ctagatccgg cattgattcg agccggccgt tttgataggc gcattagctt
    19921 gaatttgccc gacttaaaag atcgtgaggc tatacttaaa atacacgctg caggaaagcc
    19981 ccttaacagc gatgtcgatt ttgccaaaat agccaaaaaa acagttggtt ttagtggtgc
    20041 cgatttagaa aacatgctaa acgaggccgc tatacttgct gctcgtgatt ctgctgtaga
    20101 tattacaatg aaccatatcg atgaggcatc tttgaaggtc acaatgggtc ctgaaagaag
    20161 gaggttgcaa gacgagaatg aaaaaaggct cacggcttac catgaagccg ggcacgctgt
    20221 tgtggcatgt catgcgggtc agctagattc tgttgagcgc gtcactatag ttcctagagg
    20281 cggt
//
LOCUS       DAOSWN010000006        17119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_2106_length_17119_cov_4.767464, whole genome shotgun sequence.
ACCESSION   DAOSWN010000006 DAOSWN010000000
VERSION     DAOSWN010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17119
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2106_length_17119_cov_4.767464"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PLF29_00760"
     CDS             complement(<1..611)
                     /locus_tag="PLF29_00760"
                     /inference="COORDINATES: protein motif:HMM:NF015427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HXK52506.1"
                     /translation="MDEIINNLEHEKEVLQKRASEINPLLSQEKDQQMLKLYQEELSS
                     LYKQIKDISNSIGSLKGEYAEEESESENSQETINQNVATIEIRSGTGGDEAGLFAHDL
                     YRMYIRFAERMNWRVEELFYSDNEVGGIKTAIFSIRGLGAYRLLANEAGVHRVQRVPK
                     TESAGRIHTSTATVAVLPELKNVNIDIKPEDVKMDFFRSGGSGG"
     gene            complement(651..962)
                     /gene="rpmE"
                     /locus_tag="PLF29_00765"
     CDS             complement(651..962)
                     /gene="rpmE"
                     /locus_tag="PLF29_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004017962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HXK52507.1"
                     /translation="MKKDIHPQVNKNIKITCACGTVFTTSSTLDDFKVDICSNCHPYY
                     TGKQKFVDTEGRIDKFQKKLDESQKKKAAAKNKKSKEKEKVTDEPKTLKELMEEASKK
                     S"
     gene            complement(988..2805)
                     /locus_tag="PLF29_00770"
     CDS             complement(988..2805)
                     /locus_tag="PLF29_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52508.1"
                     /translation="MSNEKRSGNEPPCNYKFWNHAVTAALLRELDIDPENPINSLLCG
                     ELELYSLGSILRTNQLETIRRMGLRDTVRAWSALAQIRRENIGYGSTHRERTKHEELL
                     RELYRNGGAICAFDARIDGLAAQLYYEGLSKQGARIAAEGGGDMGPWRNTELLLLAIL
                     AGTDLIEIKKNDDLSATNIPTPIYCATNTIGRREFLFRFSLATAALGFSLARTSALPH
                     TPIKRVAPVEAIAKIKIEKEESLEPEVIFTETVFGIELRNLVMAINAIATKKIIDDAG
                     LQTNMGFFAGMAHGAIVDLWKKPREKLHQELKEFIHELVHSKLPIIMRFCEDGVNEQT
                     RVLERFANVIAGLGSLFTEPAILQQQNHKNFQLPEGPRRSEPLDTIARVLIPSYVSAR
                     QIFLQELDKTIQDLQQKHSPQQMGNSEQQFLLAGMLKARELLLYKAVDQRLSIEGLCG
                     RRTKKYLLTEIEDVTYLATTKITGRQTPFSHNRLGIHVGETSFCGVHREVFMKASFVR
                     TGESGEAQVTLEKFFYDLDGTVVVFSQQTAKKIKDTPEPDRPGRGELMVVVNKTDKTQ
                     RTAEFSGTHIVGRSARPRQGIFYIVEDRNLGKIVIRLNK"
     gene            complement(2841..3824)
                     /locus_tag="PLF29_00775"
     CDS             complement(2841..3824)
                     /locus_tag="PLF29_00775"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXK52509.1"
                     /translation="MPNRTHNNLKLRAYGALILATAIWGGALPIIKITEQYIPVFTFL
                     FLRFLLVCILMLPVMIVELKKTPIATKDIKNLVILGLLGQTALAFVFVGVKYTTSLDA
                     AILGTSAPIMVIAAGHFFYNEKLNYKAKLGVFIATLGTLLIMLEPMLFKGQHTELEQG
                     LRVLGNLAVLIYNVAFTAYIIVSKHVMGENSSTLTTILKRLRLNRMTRSYSPTLHTGF
                     TFYVSLVSIFPLMLIENRYMKAFDLTNLTIIPIAGILYMAILSSIVAYVAFEWGLKRA
                     KAADTAIFSYLGPLFTIPISFVLLQESPTPAAFLGIIIIVIGVVGAETNRK"
     gene            3873..4526
                     /locus_tag="PLF29_00780"
     CDS             3873..4526
                     /locus_tag="PLF29_00780"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXK52510.1"
                     /translation="MEITYIGHSCFKIKGKNISLVIDPYDPTMLGYKLPTLSADAVLL
                     THDHPDHANVQGVKDYRLVISAPGEYEISDVFVYGLQTFHDESEGSKRGNNTIYSIEI
                     DDFSLMHLGDLGHILSAETLEKIGTVDVLMIPVGGVYTIDSAAAVKVISSIEPGIIIP
                     MHYQTADLKMAGDKLDSLDKFLDEMGIEKPRTEGKLKLSSRSDVPAESTVTVLTPEH"
     gene            4559..5914
                     /gene="dnaB"
                     /locus_tag="PLF29_00785"
     CDS             4559..5914
                     /gene="dnaB"
                     /locus_tag="PLF29_00785"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HXK52511.1"
                     /translation="MASSNKHKASDPVNMGVPPHDLDTEKSVLGALLLDQDAIVKVVE
                     FLRPSNFYKEAHANIYQAILNLYEKRDPADLITVPNELKKMKLLEASGGVSYLTDLVN
                     SVPTAANIEHYAQLISEYSIKRSLLSVSGEIGTLVFSEPDVEELLDKSEQLLFSVSQD
                     RLHQDFFSVKDILEITFERLDELSKTRGALRGVSTGLPNLDKMLSGLREGELIILAAR
                     PSVGKSSLAINIAQHAATKNKTGVVIFSLEMGKEAIVDRMVSTQGDIDNWRIATGNLE
                     EEDFEKYSIASGELAEAPLLIDDTPGMSVLEMRTKARRLSLERDIGLIIIDYLQLIKA
                     PSAESRVQEVSKISQALKNLARELNVPVLALSQLSRAVEQRGGDKRPQLSDLRDSGSI
                     EQDADVVMFLYRPDEEDRSSLNLIVAKHRNGPTGELDLFFRGDRTKFFEVTRQKDEAQ
                     S"
     gene            5997..6083
                     /locus_tag="PLF29_00790"
     tRNA            5997..6083
                     /locus_tag="PLF29_00790"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6030..6032,aa:Leu,seq:caa)
     gene            6103..6654
                     /gene="efp"
                     /locus_tag="PLF29_00795"
     CDS             6103..6654
                     /gene="efp"
                     /locus_tag="PLF29_00795"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HXK52512.1"
                     /translation="MIATELKTGTIFKENEVPYLVLKYTHTKVARSGANVKVKAKSLL
                     NGSVIEKSYNGTTKLEEADIFRKSAQYLYKEGGYIFMDPDTFEQFTIPEDILGESAQF
                     LREGESVQVLYFEDAPISVELPISMLFEVTYTEPGFKGNTVSNVYKDATVDNGAVVKV
                     PSFINIGEKVKIDTRTGEYISRA"
     gene            6736..7653
                     /locus_tag="PLF29_00800"
     CDS             6736..7653
                     /locus_tag="PLF29_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HXK52513.1"
                     /translation="MLPAILTINTFTLSTYYIFGFIGFLFSLFIVWYEAKRDGFELEK
                     ALDIFFVSFGAAALSGYFFKLEIAYFVFWLIVYIWAKHINWSHFRLYDIFSTALYIEF
                     IWVFAGLILTNSFPDYSVQIYLFSLVLLVLFIFGFLFRVKLTSGFTFSLSTIVAGSGL
                     FLLFRSVLVLPFYISLFTISIVTLYLRQKSMSSLPQTFIDRMKSLLKRKNAQLEAEQK
                     SLLDKDPYLAPGRTEENEILDDVNEDLEKELYDKKVNFIRSLQSQVQRALARMRAGKY
                     GVCEVCGKEIGKERLEALPEATRCVQHAE"
     gene            7994..8383
                     /locus_tag="PLF29_00805"
     CDS             7994..8383
                     /locus_tag="PLF29_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXK52514.1"
                     /translation="MGDTFINKTILLVEDEDDIRSVYAEVLKDDGFTVIEASDGLSAY
                     DLAVNKFWDLMLLDIMIPKLDGIGLMKRLAREDKLDSRPVILLTNLGNDSVIAEAFDL
                     GASGYLMKAEITPDKILQEIKAYLKIS"
     gene            8444..8812
                     /locus_tag="PLF29_00810"
     CDS             8444..8812
                     /locus_tag="PLF29_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXK52515.1"
                     /translation="MAKIILIEDEQALSEVYSEVLRDAGYEVSQCFDGEEGLKTLRAG
                     SWDLLLLDLMLPKIDGIEILQQLVNEGLLEGRSVIMLTNLGSDELVNKCSVLGAKEFL
                     IKSDIDPKVLVEAVGKALSK"
     gene            8902..9591
                     /locus_tag="PLF29_00815"
     CDS             8902..9591
                     /locus_tag="PLF29_00815"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HXK52516.1"
                     /translation="MNLKDRIIFEDDLILVLDKPAGVVVNISQTSPESTVQNFVAEYL
                     NLHPYKDREDDFYQRCGIVHRLDKATSGVLVVAKTPDSFVDIQSLFKDRKIQKVYVAI
                     VHGKIEDAKIIIDAPLSRDPKRRTKFAVVKGGKESSTDISVIKNFALRGEELTEVEVK
                     PLTGRTHQIRVHLAAINSPIVGDVLYSTRKKLIWSQECGINRMLLHAKALTIPGYGEF
                     QASVPSDFNLI"
     gene            9704..10861
                     /locus_tag="PLF29_00820"
     CDS             9704..10861
                     /locus_tag="PLF29_00820"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HXK52517.1"
                     /translation="MPTTAKSLSKNKSFKAKTLRGSKGKTKASALKRNSRSLKIKSKS
                     TYKNKKIAKKRSFASYFPLFVLFVASLVILSATVMYKNYLQGFASASSISAFSLQDTD
                     IFSAAVVYVDSMPQLGKDYTPDVISAVIYIYDTNNMSINVFNIDNDLEIDMPGRLGDD
                     TLEKIYALSLMITNGDTAESDHYIKHSLSRLIGFPIDRLVVASDEDRSDVESLLLRGA
                     EISPLLIKDINLYVNEVSTDLTKQEILYIAREANKSQGNISEIDLTSDLSQSSDSLIQ
                     EITFGSEISVEGKTVAVLNGTTTVGAANDVARAIENMGGRVITVGNADRQHDTTTLLI
                     DDPDSAAARGIYRFFDISDIRLKDNPGDIIDAEVNRADIVFIVGVDMLDQL"
     gene            10931..13435
                     /locus_tag="PLF29_00825"
     CDS             10931..13435
                     /locus_tag="PLF29_00825"
                     /inference="COORDINATES: protein motif:HMM:NF021897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HXK52518.1"
                     /translation="MFELLYVLVPILTVVGIVYVLFFNRRVSKGYIVADSVFPDFKLL
                     EIQVPKNEDASKDSAISSLSAEHLFAALHGILKEESNIQEHVSLEIKGDKEGIKFYVG
                     APASILKYIESQIYAQYPQAHISVYNGHNYLGDRTGIFEVGSMSLEKDFIFPIRVFRD
                     FEIDPLANIASTLSEIKEDDQIIIQTLFKPVPDGWQEKGYKYLAAVTGGNSVESDPFL
                     KDISRSLFTEILSILKNIPVIMIRGYADEPQGQFKVREGGGVRLSAFQELEIKAVEHK
                     LTHMGFAVQMRVYIASATSGRIDDLKRSVGSAYKQFSMTGLNSMNLTLESNRRSALDD
                     FRVRQFDAYKSFILNSEELATIFHLPSASSDLPSISWVYSRKSEPPSVLPTEDCTYIG
                     DTVYRGKKVRFGLSNGDDRLRHMYLIGKSGTGKSTLMEAMVLQDIIRGEGVCLLDPHG
                     ETIDKVLDRIPDHRIEDVVVVDPSDTEMPVGINLLELSHPDQKNLMASGLVSAIRHHF
                     DYSWGPRLEYLLNYSLLTLLEVPGTSMLGVTRLLEDVNYQKYILHNVKDPVIQAFWDK
                     EYKEMRGNAKLVTEAIAPIQNKVNRFLSSSTIRNILVQRRSTIDIWDIMNSGKILLIN
                     LSKGKIGEDNANLLGALLVSRIQFYALQRANITYDKRKPFYLYVDEFQNFATGSFESI
                     LSESRKYKLGLYLTHQYTAQLPEDLQKAVFGNVGTIAAFSLGAPDARILQSEFAPYFD
                     SEDIISLERFNIYIKLMIDGMTSLPFSAKILLPWEEEGLIPKTGNREKILELSRRKYG
                     VPRDRVESAVNKWIERPFDKGMAIAEQIKRGGTNGQ"
     gene            13425..14147
                     /locus_tag="PLF29_00830"
     CDS             13425..14147
                     /locus_tag="PLF29_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52519.1"
                     /translation="MDNRDLKERLLNLKSNLAKVISEIDKIYAALDRVEDSPPAKKDP
                     SLLVAAQLPREVDLPVAPVAVEEAANAIETSYLNQVAETDAGIRGSFDGLNLVTLEGE
                     TYEVPANYAAKTKLVCGDTLIMQSHEGRNLFKVIDRVPTIEISGIAMKKDDGWFILNA
                     DKSYKISDAAADFNKLSEHCEVKVLIPENSSNCNFATFVEAVCEDENKGSTSSADTSE
                     GSSAPKDLSLGNLRVIEDDDLR"
     gene            complement(14232..14894)
                     /locus_tag="PLF29_00835"
     CDS             complement(14232..14894)
                     /locus_tag="PLF29_00835"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXK52520.1"
                     /translation="MQQSLSALEKKAIKLALCENWAEAIATNKELLDLDPTNLQAKIR
                     LGRAYLLSKDFRNASKMFKAVLEQDPINQIAKKNYELAKSKSAKVAINSGAVIKEPGT
                     SEEVKAEVISKSKIWERLAKGQTFSMRILKTKTLLIHSSKEIAQIGGPMALKIYLHTK
                     EGAKVSAQYIGQSNETKNCVDILLKADRPIFKQEKQDVRPYMKKGSIEEHEIEVDSFD
                     DE"
     gene            15074..15610
                     /locus_tag="PLF29_00840"
     CDS             15074..15610
                     /locus_tag="PLF29_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HXK52521.1"
                     /translation="MSGHSKWSNIKRKKEITDAKKSKVFSKMSRLITVAARDGGADPD
                     MNPTLRLAVDRAKQANMPSENIDKAIKKGSGALEGAKFERVVYEGYGPYGVAFMITTL
                     TDNRNRTVSEIKNIFSKAGGSLGTPGSTSYIFDSLTFEPSFRIALESEEQLKKIENLE
                     EALEDQDDVQEIYSNYEL"
     gene            15607..16113
                     /gene="ruvC"
                     /locus_tag="PLF29_00845"
     CDS             15607..16113
                     /gene="ruvC"
                     /locus_tag="PLF29_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HXK52522.1"
                     /translation="MIIIGIDPGTAKTGYAVMEYPKNYNKSSKRSGNLIECACIDTPS
                     EATMHDRLSQLYDELKNISSRTKPKMMVIEKLFFNTNIKTAMTVGQARGVSLLVASQH
                     AMEVVEYTALQAKQTLTGYGRASKKEMQEAVREYMRLEKVIKPDDANDALAMILCHVE
                     KTRIPEQN"
     gene            complement(16250..16414)
                     /locus_tag="PLF29_00850"
     CDS             complement(16250..16414)
                     /locus_tag="PLF29_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52523.1"
                     /translation="MKANPLTLLSKASATIATSIVAVSAKEIIISELTVVAYREAKNN
                     VTGVNIEATI"
     gene            16500..17024
                     /locus_tag="PLF29_00855"
     CDS             16500..17024
                     /locus_tag="PLF29_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52524.1"
                     /translation="MASFIGLGFVVYALSLVNNIFVVVEEKQSVIPLYSAGLAWVQII
                     LILIAIPFYSGIYKVPANFVVQNILLFFSTFSFYIYMHWAVNLDKDLIKTPLKEKGSL
                     AFVLSLLPVVLNTGLLFYPVEAFLKGIFSSTVLLSGLIYIQAHYKNRINSKLLYNYFV
                     LGSIFYIILFIFKP"
BASE COUNT         4965 a         3082 c         3586 g         5486 t
ORIGIN      
        1 cctcccgagc ctcctgaacg gaaaaagtcc attttcacgt cttccggctt tatatctatg
       61 tttacgtttt taagttccgg gagtacagca accgttgcag tcgacgtatg tattctgcct
      121 gcgctttctg tttttggaac cctttgcacc ctgtgaaccc cggcctcgtt agccaaaagc
      181 ctataagcgc ctaagccccg aatagaaaat atagcagtct ttatccctcc aacctcgttg
      241 tctgagtaaa ataattcctc aactcgccag ttcattctct ctgcgaatcg tatgtacatt
      301 ctgtaaaggt cgtgtgcaaa taggcctgct tcgtcccctc ctgtgcccga tctaatttct
      361 attgttgcaa cattttgatt aattgtttct tgcgaattct cgctttcgga ttcttcttct
      421 gcatactctc ctttaagcga gccaatagag ttggaaatat cttttatttg tttgtataac
      481 gaggacagct cttcctgata aagttttaac atttgttgat ctttttcttg agataacaga
      541 ggatttattt ctgaggcacg tttttgcaac acttcttttt cgtgctcaag gttgtttatt
      601 atttcatcca tatgtgaatt atagcagaga agaggtgcct gttttttctt ttagctcttt
      661 tttgaggcct cttccattag ctcttttagt gtttttggtt catctgtaac tttctctttc
      721 tcttttgatt ttttattttt tgcggctgct tttttctttt gcgattcatc aagcttcttt
      781 tgaaatttat ctattcttcc ttctgtgtca acaaacttct gtttgccagt atagtatggg
      841 tggcagttac tgcagatatc taccttgaaa tcatcgagcg tagagcttgt tgtaaaaaca
      901 gttccgcaag cacatgtaat cttaatattt ttattcacct gtgggtgtat gtcttttttc
      961 atgcaagaag tagtatagca gattatatta tttgttcaac ctaattacta tctttccaag
     1021 atttctgtct tcaactatgt agaaaattcc ttgcctgggt cttgcggatc tgcccactat
     1081 atgggtgccg gaaaattctg cggttctttg ggtcttgtct gttttgttta ctacaaccat
     1141 caactccccc cgccccggtc tatcaggttc tggagtatct tttattttct tagctgtctg
     1201 ttgagaaaaa actaccacag taccgtcgag atcgtaaaag aatttctcta gggtgacttg
     1261 cgcctctcca gattctcctg ttcttacaaa gctagctttc ataaatacct cacggtgtac
     1321 cccacagaag cttgtttctc ctacgtggat tcccaatcta ttatgtgaga agggggtttg
     1381 tcttcctgta attttagtag tagccagata agtaacatct tcgatttcgg ttaggagata
     1441 ttttttagtt cgtcttccgc ataacccctc tattgaaagc ctttgatcta cagccttgta
     1501 cagcagtagc tctctagcct ttaacatgcc ggcgagtaga aattgttgct cgctattccc
     1561 catttgttgc gggctgtgtt tttgttgtag gtcttgtatg gttttatcaa gttcttgcaa
     1621 aaaaatttga cgagcggaga cgtaacttgg tataagtact ctggcaattg tatcgagtgg
     1681 ctcgctcctt ctggggcctt ccggtaactg aaaattttta tgattctgtt gttgtaaaat
     1741 agctggttcg gtgaataggc ttccaagccc tgcgatcacg ttcgcaaatc tctccagcac
     1801 ccgtgtttgt tcattaactc catcttcgca aaatctcatt attatgggca gtttactatg
     1861 tactagttcg tgtataaatt ctttgagttc ttggtgaagt ttctctctcg gttttttcca
     1921 gagatccact atcgccccat gggccattcc tgcaaagaaa cccatatttg tttgtaaacc
     1981 ggcatcgtca ataatttttt tagtcgctat agcatttatt gccattacaa gatttctaag
     2041 ctctatcccg aataccgttt ctgtaaaaat aacttccggt tctaaacttt cttctttttc
     2101 tatttttatt tttgcaatag cttccacagg ggcgactctt tttattggcg tgtgtggaag
     2161 tgctgaagtt cgtgccaaac taaaacctaa ggcagcagtt gccaacgaga atctgaataa
     2221 gaattctctt ctacctattg tattcgtagc gcagtaaatt ggggttggta tatttgttgc
     2281 agataagtcg tcattttttt ttatttcaat taaatctgtt cctgctaaga tagctagtag
     2341 caataattct gtgtttctcc agggacccat atcgccacct ccttcagcag cgattcttgc
     2401 accttgttta ctaagtccct cgtaataaag ctgggcagcc aggccatcta ttctggcatc
     2461 aaaagcacat attgcgccgc cattacgata aagctcgcga agaagttcct cgtgcttcgt
     2521 acgttctcta tgtgtcgatc cgtatccgat gttttctctt ctgatttgtg ccagagcact
     2581 ccaagctctc acagtgtctc tgaggcccat tctacgtatg gtttctagtt ggttggttct
     2641 aagaatagat ccaagagaat ataattccaa ttctccgcaa agaagagaat ttatgggatt
     2701 ttctggatct atgtctaatt cccttagcaa tgctgcggtt acagcatgat tccagaattt
     2761 ataattgcag ggtggttcat ttcctgatct tttttcatta gacatgtttt tgttgttaat
     2821 gaagccaaaa tggctctagt ttactttctg ttggtctctg ctcctacaac acctattaca
     2881 attattatta tgcctaaaaa tgcagccgga gtaggggatt cttgcaaaag tacaaacgag
     2941 ataggtatag taaaaagagg ccctaaatag ctgaaaatag cagtatctgc ggctttggct
     3001 ctttttagcc cccattcaaa cgctacatac gcaacaatag aagacaagat agccatatac
     3061 aaaataccgg ctataggtat tattgttaga tttgtaagat caaaggcttt catataccta
     3121 ttctcgataa gcattaatgg aaatattgat acaaggctca cataaaaggt aaaaccggta
     3181 tgaagggtag gggagtaact tcgtgtcatt cgattaagcc ggagtctctt taagattgtt
     3241 gtcagggttg atgaattttc gcccatgacg tgcttggata caattatata ggctgtgaaa
     3301 gcaacattat aaataagcac tgccaggttg cctaaaactc gaagcccctg ctctagctcc
     3361 gtatgttgac ctttaaacag catcggctcg agcattatta gcaaagtacc cagagttgca
     3421 ataaaaactc ccagctttgc tttatagttc agtttttcgt tgtagaaaaa atgacccgca
     3481 gcaataacca taataggtgc agatgttcct agaattgcag catccagaga tgttgtgtat
     3541 tttacaccca caaatacaaa tgccagcgct gtctgtccaa gtaaacccag tattacaaga
     3601 ttttttatat cttttgttgc tatcggagtt tttttaagtt caacaatcat cacaggcaac
     3661 ataaggatgc atacaagcag aaagcgtaaa aataaaaatg taaaaacagg tatatactgc
     3721 tctgttattt ttataatcgg aagggctccg ccccatattg cggtagcaag tattaatgct
     3781 ccgtaggcac gaagtttaag gttgttgtgt gttctattag gcattgtttt aattatacat
     3841 aaatcgaaaa agttatgtga taatatatca atatggaaat tacatacata gggcattcgt
     3901 gctttaaaat caaaggaaaa aatatttcat tagttattga cccttacgac cctactatgc
     3961 ttggttacaa attaccgact cttagtgctg acgctgtttt attgacacac gatcatccgg
     4021 accacgcaaa tgttcaaggc gtgaaggatt acaggttagt tatttcggct ccgggagagt
     4081 atgaaatttc tgatgttttt gtttatggac ttcaaacatt tcatgatgaa tcggaaggct
     4141 ccaagcgagg caataatact atttattcaa tagaaataga tgattttagt ttaatgcatc
     4201 ttggagactt ggggcatata ttatctgccg aaaccttaga aaagataggt actgtagatg
     4261 ttttgatgat cccggtgggc ggagtttaca ctattgattc tgcagctgcg gtaaaggtaa
     4321 tttcttcaat agaaccgggg atcattattc ccatgcatta tcaaacagcg gatctaaaaa
     4381 tggccggaga caaattagac tccttggata agttcttaga cgagatgggt attgaaaagc
     4441 caagaactga agggaagtta aagttgtcat cgcgtagcga tgtgccggct gagtctactg
     4501 tgactgttct aaccccggaa cactaaaagt attcgtagtt ttcgtgagag gttaatttat
     4561 ggcaagctct aataaacaca aagcaagtga cccagtaaat atgggagttc ctcctcatga
     4621 tcttgataca gaaaaatctg ttttaggggc gttattgctg gatcaagatg caattgttaa
     4681 agttgttgaa tttttaagac cctctaattt ttataaagaa gcccacgcta atatttatca
     4741 ggctatacta aacttatacg aaaaaagaga tccggccgat cttataactg ttccgaacga
     4801 gcttaaaaaa atgaaactgc tagaagcttc aggaggcgtg tcttacttaa cagatcttgt
     4861 aaactctgtt cctactgcgg cgaacataga gcactatgct cagttaattt cagagtattc
     4921 tataaagaga tctttgcttt ctgtgtctgg agagatagga acacttgtgt tttccgaacc
     4981 cgatgttgaa gagcttttag acaaatccga acaacttttg ttttcagttt cacaagaccg
     5041 ccttcatcaa gatttttttt cagtcaaaga tattttggaa attactttcg aaagattaga
     5101 tgagttgagt aaaaccagag gtgctttgcg tggtgtttca accgggctgc caaaccttga
     5161 caagatgcta tcaggtttaa gagaaggaga gttaattatt ttagctgcac gtccgtctgt
     5221 aggaaaatca agtttggcta ttaatattgc ccagcacgcg gcgacaaaaa ataaaacggg
     5281 agttgtaatt ttttctttag aaatgggtaa agaggcaatt gttgaccgca tggtttctac
     5341 acaaggagat attgataact ggcgaattgc caccggtaat cttgaagaag aggattttga
     5401 aaaatattct atagcttcgg gggagcttgc tgaggcgcct ttgctgatag acgacactcc
     5461 tgggatgagc gtgttggaaa tgagaaccaa agcccgcagg ctaagtttgg agagagacat
     5521 tggtcttata ataattgact acctacagct tattaaagca ccttcagccg agagccgagt
     5581 tcaagaggta tctaagattt ctcaggcttt aaaaaatctc gccagagagc taaatgttcc
     5641 tgttcttgct ttatctcagc tttcaagggc tgtcgaacag cgtggaggcg acaagcggcc
     5701 ccaactaagt gaccttagag actcaggttc tattgaacaa gatgcagatg tcgttatgtt
     5761 tttgtatcga cctgacgaag aggatcgttc gtcgcttaat ctaatagttg caaagcaccg
     5821 taatggacct accggagagc ttgatctgtt cttcagaggt gatagaacga agttttttga
     5881 agtaacaagg cagaaagacg aagctcaaag ctaaccgctg tatttttgtg atgccggtaa
     5941 cgcacggtgt cgacattctc caaataactt gatataacat atacgcaaat gttcgtgccg
     6001 tggtggcgga atggtatacg cgttggtctc aaaaaccaat gggatttttt cccgtgtggg
     6061 ttcgactccc acccacggca ccagtcacag aaaggggaca atatgatagc aacagaacta
     6121 aaaacaggca caatattcaa ggaaaacgaa gttccttatt tagtcttgaa gtacacgcat
     6181 actaaagttg ccagatcagg ggcaaatgta aaggttaaag caaagagcct tttaaacggc
     6241 tccgttatag agaaaagtta taacggcaca acaaagctgg aagaagcgga tatatttaga
     6301 aaaagcgccc agtatcttta taaagaaggc ggttatattt ttatggatcc tgatactttt
     6361 gagcaattta caattcctga agatatatta ggagagtcgg cgcagttttt gcgagaaggg
     6421 gagagtgtgc aagtacttta ttttgaggac gcccctattt ctgttgagct tccgataagt
     6481 atgctttttg aagtaactta caccgagcct ggttttaagg gtaacactgt ttcaaatgtc
     6541 tataaagacg ctacagttga taacggggct gttgtgaaag ttccttcctt tattaatatt
     6601 ggcgaaaagg ttaaaataga tacaagaaca ggcgagtaca tttccagagc ttaggaatgg
     6661 tatttatttg gagatgtttc tgagcaatat ttgctcatgt tcgatttgcc gcacctcgaa
     6721 ctattctaat aacttatgtt acccgctatt ttaactatca acacttttac tctttctacg
     6781 tactatattt ttgggtttat aggattcttg ttctcacttt ttattgtttg gtatgaggcg
     6841 aagcgagatg gtttcgagct tgaaaaagct ttagatattt tttttgtgtc ttttggggct
     6901 gcagcacttt ccgggtactt ttttaagctt gagatcgcat actttgtttt ttggcttatt
     6961 gtttatattt gggctaagca tattaattgg tcacacttta ggttgtatga tattttttct
     7021 actgctctgt acatcgaatt tatttgggta tttgcagggt taatattaac taacagtttc
     7081 ccggactatt cagtgcagat ttacctgttt tccttggttt tactggtttt atttattttc
     7141 ggttttttat ttagggtaaa acttacttcc ggttttactt tctcgctttc aaccattgtg
     7201 gccggctcag ggctgtttct tttatttcgc agtgtgctgg tattgccttt ttacatatcc
     7261 ttatttacaa taagcatagt aactctttat ttaaggcaaa aaagcatgag tagtctacca
     7321 caaactttta tagacagaat gaaatctctt ttaaaaagga aaaacgccca gcttgaagct
     7381 gagcaaaaat ctctattgga taaagatccg tatttagctc caggaagaac cgaggagaat
     7441 gaaatattgg atgatgtcaa cgaagatctt gaaaaagagc tttacgataa aaaagtgaat
     7501 tttattcgtt ctcttcagtc ccaagttcaa agagccttgg ctcgaatgcg cgcagggaag
     7561 tacggtgtgt gcgaggtctg tggtaaggaa ataggcaaag agcggttgga agctcttcca
     7621 gaggctacaa gatgcgttca gcatgctgag taacttagct caatctgtgt tttttctcaa
     7681 ccctaattgc aagggcgttg cgtttcttct cattaatcca tatgtcggtt ttgtgttaaa
     7741 cgcgttgtcg ttgttggtta taattattct tcttagcacc cggtttagaa atcgtggtga
     7801 aaggtttttt gcgctgctta ttttttccgg aggattgttc catctcttat cgcgtttagt
     7861 gtcggggtgt gtttatgatt actttaatgt ttttggaata agctttgact tggccgatgt
     7921 atttataaat ttagggcttt ttggtttaac actgtgtatt ttaaaacaga aataattagg
     7981 ttaatatatt aacatgggtg atacatttat aaacaaaaca attctccttg tagaagatga
     8041 agatgatata agatcagtat atgctgaagt gcttaaagac gacggcttta ctgttataga
     8101 agcttcagac ggcttgtcgg catatgattt ggccgtgaat aaattttggg atttaatgct
     8161 tttggacatc atgatcccaa aacttgacgg tataggttta atgaaaagat tggctaggga
     8221 agataagctg gattcacgcc cggtaatttt gcttacaaat ctaggtaatg acagtgttat
     8281 agctgaagct tttgacttag gtgcctccgg ttacttaatg aaggcggaaa ttacgccgga
     8341 caagattctg caggagataa aggcttatct aaaaataagc taatttgatt cagatttttg
     8401 ttataataac ctttggtatt aatatatgag gaaaggaagt aagatggcga aaataattct
     8461 catagaggat gaacaggctc tttcagaggt atattccgaa gtattaagag atgcggggta
     8521 tgaggtgtcg caatgctttg acggcgagga aggcctaaag actttaagag caggttcttg
     8581 ggatttactt ttgctggact taatgttgcc taaaattgat ggaatagaga ttttgcagca
     8641 gcttgtgaat gaaggcttgc ttgagggtag aagtgttata atgctgacaa atttaggcag
     8701 cgatgaactt gtaaataaat gttcggtact tggcgcaaaa gagtttttga tcaaatctga
     8761 tattgaccct aaggtgttgg ttgaggctgt aggaaaggcg ctctcaaaat aaactcggat
     8821 ttactagaga attttatcca attcaataag aacgttttta catctttaag attgcctttg
     8881 cttctttcgc taaaataatt tatgaactta aaagacagaa taatttttga agatgatttg
     8941 attttagttt tagacaaacc ggcaggtgtt gtggtgaata tatctcaaac ctctcccgag
     9001 tcaactgttc agaactttgt ggcggaatat ctaaatctac atccatataa agatagagaa
     9061 gatgattttt atcaaagatg tggcattgtg cataggctag ataaagcaac ttccggagtt
     9121 ctggttgtgg caaagacacc ggattctttt gttgacattc aaagtttatt taaagataga
     9181 aaaattcaaa aagtttatgt agccattgtt cacgggaaga tagaagacgc aaaaattatt
     9241 attgacgcgc ccctttccag agatccaaaa agacgaacaa agtttgcagt agttaaggga
     9301 ggcaaagaaa gttccactga tatatcggtt attaaaaact ttgctttaag gggcgaagag
     9361 ttaactgaag tcgaagttaa acctctaaca ggcagaacac accaaatacg tgtgcatttg
     9421 gccgcaatta atagcccgat agtgggagat gttctatatt ccacacgtaa aaaactgatt
     9481 tggtcccagg agtgcggcat taatagaatg ttattacatg caaaagcact aactattccc
     9541 ggatacgggg agttccaagc ttcggtgcct tctgatttta acctaattta aagagatctg
     9601 atttacatga taatgtgtga gatggccaaa acatacttaa atggtctgtt ctaggttgga
     9661 taactttaca caacatgggt tattgcagta gaatcaaaac agtatgccca ctactgccaa
     9721 atcgctaagc aaaaacaaat cttttaaagc aaaaacctta agaggttcta aaggtaaaac
     9781 aaaggcatct gcgttaaaaa gaaactcacg atccttaaag attaaatcta aatccactta
     9841 taaaaataaa aaaattgcta aaaaaagaag ttttgcatcg tattttcctt tatttgtgtt
     9901 gtttgttgcg tcattagtaa ttttatctgc aactgtcatg tataaaaatt atttacaagg
     9961 atttgcttca gcatcttcta tttctgcatt ttctctgcaa gacaccgaca ttttttcagc
    10021 ggcggttgtt tatgtagact ccatgccaca gctaggtaaa gactatactc cagatgtaat
    10081 ttcggcagta atatacattt atgacacgaa caatatgtca ataaatgttt ttaacatcga
    10141 taatgattta gagatcgaca tgccgggcag gcttggggac gacacattag aaaaaattta
    10201 tgcgctttct ctaatgataa caaacggcga cacggcggaa tcggatcatt atattaagca
    10261 ttctctatcc agattaatag gttttcctat agacaggcta gttgttgctt cggatgaaga
    10321 tcgttctgac gtagagagcc ttctattaag aggggcagaa atttcaccct tgctcataaa
    10381 agacataaat ttgtatgtaa atgaggttag caccgatcta accaaacagg aaatacttta
    10441 cattgcaaga gaagcaaata agtcccaagg aaacatttcg gaaattgatt taacatctga
    10501 tttgtctcaa agttctgata gtttgattca ggaaattaca tttggatcgg agatctcggt
    10561 tgagggaaag accgtagcag ttttaaacgg tacaacaaca gtaggtgcgg caaatgatgt
    10621 tgcacgggca atagagaaca tgggaggcag agttattacc gttggaaatg cagataggca
    10681 gcacgacaca accactcttt tgattgatga cccggatagt gctgcagcaa gaggcatata
    10741 ccgtttcttt gacatttctg atataaggct taaagataat cctggagata ttatagacgc
    10801 tgaagttaat agagccgaca tagtttttat tgtcggagtt gacatgctag atcagctttg
    10861 agagacttag acaagagtct ttcgagcctc tttgttttgt cgaaaagtcc gatccacatc
    10921 acctaaaaac atgtttgaat tgttatacgt attagtgccc atacttactg ttgtaggtat
    10981 tgtatatgtc ctttttttta atcgtagagt ctcgaaaggc tatatagttg ccgattcggt
    11041 ttttcccgat tttaagctac ttgagattca ggttcccaag aacgaagatg cgtcgaagga
    11101 ttccgcaatc tcttctttat cggccgaaca tttatttgca gctctgcacg gaatactaaa
    11161 agaagaatca aatattcaag agcacgtatc tttggagata aaaggtgata aagaaggtat
    11221 taagttctac gttggtgccc ctgcaagtat tttaaaatac atcgagagcc aaatttatgc
    11281 ccaatacccc caggctcata ttagtgttta caacggccat aactatttag gcgatcgcac
    11341 aggtattttt gaggtcggat ctatgagctt ggaaaaagat ttcatttttc caataagggt
    11401 ttttagggat tttgagatag atcctcttgc taacattgct tcaacactct ctgaaattaa
    11461 agaagatgat caaataatta ttcaaacgct ttttaaacct gtgcccgatg gttggcaaga
    11521 gaagggctat aaatatttgg cggctgttac aggaggaaat tctgtagaat ctgacccttt
    11581 tttaaaagac atttctcgca gtttatttac ggaaatatta agtatattaa aaaatattcc
    11641 tgttattatg attcgtggct atgcagatga accacagggc caatttaagg taagagaagg
    11701 aggcggggta aggctctcgg cttttcaaga gttagagatt aaggctgttg agcacaaact
    11761 cacgcacatg ggctttgcgg ttcaaatgcg cgtgtacata gcctcggcga cttccggaag
    11821 aatagatgac ctgaaacgtt cggttggctc tgcgtataaa cagttcagca tgacaggtct
    11881 taacagcatg aacctgacat tggagtcgaa caggcgctcg gcattagacg actttagagt
    11941 aaggcaattc gatgcttata agtcttttat tctaaattcc gaagagttgg cgactatttt
    12001 tcatctcccg tcagcctctt cggatcttcc aagtatttcg tgggtatatt ccagaaaatc
    12061 agaaccgcct tcagttttgc ccaccgaaga ttgtacttac attggcgata ctgtgtaccg
    12121 cggaaaaaaa gttcgttttg gcctttctaa tggagacgac aggcttcgcc atatgtatct
    12181 tattggtaaa tcaggtaccg gaaaatctac tttgatggag gcgatggttt tgcaggatat
    12241 tataagaggg gagggggtct gtttacttga tcctcacggc gagactattg ataaggtttt
    12301 ggatcgtatt cccgatcata gaattgaaga tgtagtcgtt gttgaccctt ctgatacgga
    12361 aatgcccgtc gggataaatt tattagagtt gtcgcatccc gatcaaaaaa atttgatggc
    12421 ttcagggttg gtttctgcaa ttaggcatca ctttgattat tcttgggggc ccaggctgga
    12481 gtatttatta aattactcac ttttaacatt gttggaggtt cccggaacat ctatgcttgg
    12541 cgtgacaagg ttattggagg atgtaaacta tcaaaaatat attttacaca atgtaaaaga
    12601 tcccgttatt caagcatttt gggacaagga atacaaggaa atgaggggca atgctaagtt
    12661 agttactgag gctatagcgc caattcagaa caaagtaaac cgctttctat cttcaagcac
    12721 aattagaaat attcttgttc agagaagatc cactattgat atttgggaca taatgaattc
    12781 gggcaaaatt ttgttgataa acttatcaaa aggaaagata ggagaagaca atgccaatct
    12841 cttaggggct ttgttggttt ctcgaattca gttttacgct ttacaaagag caaatataac
    12901 ttacgacaag cggaagccct tttatcttta tgtagacgag tttcaaaatt ttgccaccgg
    12961 cagctttgag tcaattctat ctgaatcgag aaaatataag ttaggtttat acctgactca
    13021 tcagtacacc gcacagcttc ccgaggatct ccagaaggcg gttttcggta atgttggtac
    13081 aattgcggct ttttcacttg gagccccgga tgctcgcata ttacaaagtg agttcgcccc
    13141 gtattttgac tccgaggaca tcatttctct agagagattt aatatataca ttaaacttat
    13201 gattgatggc atgacttctt tgccgttttc agctaaaata ttattgccat gggaagaaga
    13261 aggtctcata ccaaagacag gcaatagaga gaaaattctt gagctcagtc gacgtaagta
    13321 cggggtgccg agagatcgcg tagagtcagc tgtcaataaa tggattgaaa ggccttttga
    13381 caaaggcatg gcaatagctg aacaaattaa gagaggtggt acaaatggac aatagagatt
    13441 taaaagagag gcttttaaat ttaaaatcaa acctagcaaa agttatttcg gagatagata
    13501 aaatttatgc ggctttggat agggttgaag attcacctcc agcaaaaaag gacccaagtc
    13561 tcttagtggc cgcgcagctt ccgagagagg ttgacctccc ggtggcaccg gttgccgttg
    13621 aagaagccgc gaacgcgatt gaaacatctt atttaaacca agttgcagag acggatgccg
    13681 gcattcgagg tagttttgac ggcttaaact tggttactct agaaggggaa acttacgagg
    13741 ttcctgcaaa ttatgcagca aaaacaaaat tagtttgtgg agacactcta attatgcagt
    13801 cgcatgaagg tcgtaactta tttaaggtga tagacagggt tccgaccata gagatttcag
    13861 gaatagcaat gaaaaaagat gatgggtggt ttattttaaa cgccgacaag tcctataaaa
    13921 tctcggatgc tgctgctgac tttaacaagc tcagtgagca ctgtgaggtt aaagttctaa
    13981 taccggaaaa ttcaagtaat tgtaattttg caacttttgt cgaggcagtg tgtgaggacg
    14041 aaaataaagg ttctacatcc tccgctgata cttcggaagg tagttcggcc cccaaagacc
    14101 tttctttagg aaatcttcgc gtcatagaag atgatgatct tcgttaagta agatatattt
    14161 acatgcctga gagtctctaa gagtttttct ataatgttag tctaaagatt gtagcttttt
    14221 tcttctgagt attattcgtc gtcaaaacta tcaacttcaa tttcgtgctc ttcgatagag
    14281 ccttttttca tgtatggcct aacatcctgt ttttcttgtt tgaaaatagg cctatcggct
    14341 ttaagcagta tatcaacaca gttttttgtc tcatttgatt gaccgatgta ctgtgcgctc
    14401 accttcgcgc cttcctttgt gtgaagatat atttttaggg ccatgggacc tccgatttga
    14461 gctatttctt ttgaactgtg tattagaagt gttttagttt ttaaaattct catactaaac
    14521 gtttgtcctt ttgccagcct ttcccatatc tttgatttac ttataacttc agctttaact
    14581 tcctcagatg tcccgggctc ttttattact gcgccggagt taattgcaac tttagctgat
    14641 tttgattttg cgagttcgta gttcttttta gctatttgat ttatggggtc ttgttcaaga
    14701 accgccttaa acatcttgct ggcatttctg aaatctttag atagtaaata tgcccttcca
    14761 agtctaattt tagcttgaag gttcgtggga tcaaggtcta acagctcttt gtttgtggct
    14821 attgcttctg cccagttttc acatagcgct aacttaatgg cctttttttc aagtgctgat
    14881 aagctttgtt gcatgtgtat gaagttatca aaaatacacc aaaagtcaat aaacagtttt
    14941 aagtgtttgg ccaaaaaagc ttttataaaa gctgaaattt aaaaatagat tttgctgtca
    15001 gacttttcct agtagcgcct taaataaatt tttcggactg ctttagagaa taaagcgtgg
    15061 tagaatatta tgtatgtcgg ggcattcaaa atggtcaaat attaaaagaa aaaaggaaat
    15121 taccgacgca aagaagagca aagttttttc caaaatgagt cgattaataa ctgttgctgc
    15181 aagagacggg ggcgcagacc ctgatatgaa ccctactttg cgattagcag tagatagggc
    15241 caagcaagcg aatatgccga gtgaaaatat agataaagct attaagaaag gctcaggggc
    15301 tttagaaggt gctaaatttg aacgagtagt gtatgagggt tacggaccct atggtgtggc
    15361 tttcatgata acgaccttga ccgataatag aaacagaaca gtgtcggaaa ttaaaaacat
    15421 tttcagtaaa gctggaggtt ctcttgggac cccgggtagt acatcgtata tttttgattc
    15481 tttaaccttc gaaccctcat tcagaattgc actagagtct gaagagcagt taaaaaaaat
    15541 agaaaatttg gaagaagcac ttgaagatca agatgatgtg caagaaattt actcgaacta
    15601 cgaattatga taattatagg aatagatcca ggaacggcta aaaccgggta tgcagtaatg
    15661 gaatacccta aaaactacaa taagagctcg aaaagatcgg gcaatttaat tgaatgcgcg
    15721 tgtattgata ctccttcgga ggcaacgatg cacgatagat taagtcagtt atacgatgag
    15781 ttgaaaaata tatcttctag gacaaaacca aaaatgatgg ttattgaaaa gctctttttt
    15841 aacacaaata ttaaaacagc aatgacggta gggcaggcaa gaggggtatc tcttttggta
    15901 gcttcacagc atgccatgga ggttgttgag tatacagccc tgcaagccaa gcagaccctt
    15961 acgggatatg gacgtgcctc aaagaaggag atgcaagaag cggttcgaga gtacatgcgg
    16021 cttgaaaaag tcattaaacc cgacgatgcc aatgacgcct tagcaatgat tttgtgtcac
    16081 gtagagaaaa cacgcattcc cgaacaaaat taagtataat aatagttatg gatcccttaa
    16141 ctcatttaaa gaacgaagta tcaagactct ttattgaaag aaggaagagt ttaggctata
    16201 agggagccct tctacagttt tttaatgatc ttataaacaa gaggtccatt tatatcgtgg
    16261 cctctatatt aactcccgta acattatttt ttgcttcgcg gtacgcaaca actgtaagtt
    16321 cgctgataat tatctccttc gcacttacgg caacgatact ggtagcgatt gtggcactcg
    16381 ccttgctcag cagggtgagg ggattcgctt tcatatactt tttgataccg gtttcctatt
    16441 tggtattagg ggttctattc tctttatatt ttttccctaa tttaggatat ttaataaaaa
    16501 tggcatcttt tattggtctt ggttttgttg tctatgctct ttctcttgtc aataatattt
    16561 ttgttgtggt cgaagaaaag cagtctgtta ttccgctata cagcgccgga ctggcttggg
    16621 ttcaaattat tctcatttta atcgctatac ctttttattc gggaatatat aaagtacccg
    16681 caaattttgt tgtacagaac attttgttat ttttttcaac cttttctttt tatatataca
    16741 tgcattgggc ggttaattta gataaggacc taattaaaac cccacttaaa gagaagggaa
    16801 gtttagcttt tgtattatcg ttgcttccgg tcgttttaaa taccggcctg ctattttacc
    16861 cggtagaagc ttttctaaag ggtattttta gctccacagt gttgttgtcc ggcttaattt
    16921 atattcaagc acactataaa aatagaataa attctaaact tttgtataat tattttgtct
    16981 taggtagcat tttttatata attcttttta tatttaaacc ttgattatcc ttttaatacg
    17041 gcctttagat cagggaacag ggaactatat tctaatgtag attgcacatt tgtcataaac
    17101 ccgatcacta tagggtagc
//
LOCUS       DAOSWN010000007        14641 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_2596_length_14641_cov_4.590772, whole genome shotgun sequence.
ACCESSION   DAOSWN010000007 DAOSWN010000000
VERSION     DAOSWN010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14641
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2596_length_14641_cov_4.590772"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PLF29_00860"
     CDS             complement(<1..432)
                     /locus_tag="PLF29_00860"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HXK52525.1"
                     /translation="MSSVYSSVDKNKRETAVIFIGFIFFVTAVFYFASLAYFGDSSML
                     VFAFLFSFVSGFVGYYKSDSLVLAISGAREIKPEDSQYVHSLVENMCIASGLPKPKLY
                     VINDTALNAFATGRSPDKGVICFTSGIIDRLEKRQLEGVIAH"
     gene            complement(451..1002)
                     /locus_tag="PLF29_00865"
     CDS             complement(451..1002)
                     /locus_tag="PLF29_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HXK52526.1"
                     /translation="MSILSILLLIPLAIVLYVVALYNMFVKLRTRVEESWSQIDVQLK
                     RRVDLIPNLVETVKGYASHEKDVFENVTKARASLMSAVTPAEVGAADNQLSQTLKSLF
                     AVAEAYPELKAQEGFVNLQKELSDTEDKVSYSRQFYNATVRDLNQKIDMFPSNLVASI
                     FNVKKREYFEATEQEKEAVKVAF"
     gene            complement(1017..2759)
                     /gene="polX"
                     /locus_tag="PLF29_00870"
     CDS             complement(1017..2759)
                     /gene="polX"
                     /locus_tag="PLF29_00870"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HXK52527.1"
                     /translation="MLKNPELVHILKQMLAAMEVKDYNKFRIRAYQNAIASIESLSTS
                     VYTLWQQGRLSEIPGVGGGLTQVFKELFTTGEVLEFNRLKADLPAGMFALIGIRGIGS
                     KKAFKLASAFALDDESTAVEEIKKIAEAGKIRDLPGFGERSEAELLAVITEMKLHKKE
                     KERSLFVVAEQVAKRLEYYLRLCPEVSALEFLGSYRRREATVGDLDIAIVTAKPDEVF
                     DHFEKYEEIEDVLVKGEKKMSVNLTNNMQIDIRTVSHEELGSMMQYFTGSKAHNIVLR
                     THALEQDKSLSEYGIKHGKSLKTFSREEDLYSYLGLSYIEPELRQGKDEVVLAQKKAL
                     PELVNLEDIKGELHVHTNFSDGANTLEEMVHAADALGYEYFGVADHAPSVSNRGLKEV
                     MNITNTHIKSVEHINSSTNLKVLLGYEVNILANGSISLPDESLSKLNFAIGSIHTAFD
                     QDRDTITQRLINAIEHPLINIIGHPSGRLLNERPACDVNWTAVFKSAIEHDKVIEINA
                     QPNRLDLADDLVREACAMGVKIMINTDAHSTAGLNFMRYGVYVARRGWCSKEKILNTL
                     PLKEFLRELNIRNI"
     gene            3060..3136
                     /locus_tag="PLF29_00875"
     tRNA            3060..3136
                     /locus_tag="PLF29_00875"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3094..3096,aa:Asp,seq:gtc)
     gene            3214..3831
                     /locus_tag="PLF29_00880"
     CDS             3214..3831
                     /locus_tag="PLF29_00880"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HXK52528.1"
                     /translation="MNTETKKDFLALVIALIFVGSVSYFGYSYFNQGAQQNKGEIGSS
                     ASTKRTETEETESYGSKLYKIKTKVNELIEGSEEIQERDSSETTTNYKEEANYSEKNE
                     DSAQPGDGAEEKYTTPQVAQPHSNEWTATDYVKGDIKVQNNKYEVKSGDTLWEIAEAV
                     YGNGSEWVNILSVNADNIGYLNNGQQALIFPGQIITIPVNAGTAI"
     gene            4081..4156
                     /locus_tag="PLF29_00885"
     tRNA            4081..4156
                     /locus_tag="PLF29_00885"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4114..4116,aa:Thr,seq:cgt)
     gene            4180..4896
                     /locus_tag="PLF29_00890"
     CDS             4180..4896
                     /locus_tag="PLF29_00890"
                     /inference="COORDINATES: protein motif:HMM:NF039533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HXK52529.1"
                     /translation="MAQKRHYHEEILIKRLKRTILAVIGIPVLVIAIMAFFGPTVASI
                     FGFVSIDRWTKAPGDTIAPTVPGFRSPPGSVKDTKITLNGFGEPGSVIKLFINGPEKQ
                     STVVGNDGTFTFQDVELINGKNTLFLKAQDQAGNESEASERVEITVDTIEPKIEITAP
                     EKDKTVKNLNERVLIQGKISEKATIRINDRLAVQKPDLTFEFILGVKQGEVEVTVEAV
                     DEAGNKKEEKLKFTYLKQSE"
     gene            complement(4864..6030)
                     /locus_tag="PLF29_00895"
     CDS             complement(4864..6030)
                     /locus_tag="PLF29_00895"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HXK52530.1"
                     /translation="MLGQLAKLSYFGGPGIYFFDIYIAFSALYCVFYLLLSGKKLYVE
                     SVLFPFAVFSTWALVSLLVTPVSMIPEEFSESFLYFVRFSSYLLFGFCVASSVKNGLV
                     CHIRLEKAIVFSGLLFIILGFIQLYFLPDFGVLERSLGWDPHKFRLASTFFDPNFAAT
                     YLCIILLVLIRIRSFFSRNILGLFFLLCLIAIFLTFSRSGWLFLACIGLVYGVFRKPR
                     MLLVSIFICSLVFFTVPRVQGRILGVTNPADSASFRWRSWENGLDLFKSNPVSGVGFN
                     TLRYAQKENNLLSFIDYSIEDGGNSGAGIDSSFIFVLATTGVVGFGLFLIGFLGPLAK
                     LAKGPSAFPAEKSLYYISLIVGLGVSSQFINSLFYPQIMYLFFLLLALFEIGEL"
     gene            complement(6059..7366)
                     /locus_tag="PLF29_00900"
     CDS             complement(6059..7366)
                     /locus_tag="PLF29_00900"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HXK52531.1"
                     /translation="MIVTKLLKLTGPSSTKGVVASNALYQIIGKFLSMGITVFVTVLV
                     SRLYGREGYGEFNLMQSWPALFFIIVDFGFNAVVIRDVKKDKSQLETYLANVIGLRLV
                     ISFLIILVALGVLSFFPYNRLLKIGIILNLCLVVTQALYASANIIFQKYLRYDLSTIS
                     YILGYGAILITALVLIHFKQNVMWVSFAYVVGGSITFLAQYFLLKKFFFIRARIRLQK
                     EIYKPLLIQAVPLGLMFVFSQINFKSDAILMSVLPVPAFIKLSQTEAVGVYGLPYKIF
                     EVALVLPTFFMNAVYPIFIQKLETGKEDFLLMFYKTIGFLFLLGFAGAAVGAGLSGYV
                     INLLGGQEFLQSAGVLRLLLIGLPIFYISQPISWFIVSLGKQKYLPAVYLLGAVFNVT
                     LNLITIPMYSFYASSVLTWLSELFIVILLVIFARKSWKTYVAR"
     gene            complement(7353..8534)
                     /locus_tag="PLF29_00905"
     CDS             complement(7353..8534)
                     /locus_tag="PLF29_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52532.1"
                     /translation="MRHILTQVLYVILFSLISLLFTIFIYILREAFVSHTLPEVFIFN
                     TKLPKQDAGCTSLEFNYQYYKDAIGNDNNNIYGIYVYAEDTEFLDLAAELVNSNGGDW
                     GYVLIPFNISTDRDFEKWRKVFEVLAEKHLIPIIQLNALDLDEIPRDTKRGAEFLNFF
                     DWPVKDRYISVYNEPNDSNFWYGKADPKQYAEILDTTIDIFKDINSNYFMLNGALNIS
                     ASGGNGTIDALDFINQMYSSRPEVFNKLDGWASHSYPQPNFSGSPYGSGRNSVRAYEF
                     ELSYLSSVLGISKEYPIFITETGWAHAEGLSYNPSYYTSKRVGENFKIAYEQVWLKDK
                     RIKAVIPFTIWYPPPFDHFAWVDVDKEPFEQFFEVKSMKKVAGTPETLFTGRIESNDC
                     N"
     gene            8646..9869
                     /locus_tag="PLF29_00910"
     CDS             8646..9869
                     /locus_tag="PLF29_00910"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HXK52533.1"
                     /translation="MDLLKKLIIILYATSLGLMTVNFIFVKTPKTYNIKSPVHGSFSV
                     LAAVDQQRENYIVYGFLPYWKLDNAKFIQTDKLTDIAYFGLKIEGNGEIRRFEEPGVL
                     EPGYNNWKNNPELSNLISRSKKAGVRFALTIISHEDEVSDEFLNCTSCWQTLINEIGI
                     ELEEKNITDVNLDFEYFGYTDLETALKYSAFAKQLNDTLDARYNNNSKVIVSTFADSV
                     VKPRVTHIDTLAKYTDGLFVMAYDFHRPASATAGPIAPLGGMGDKSSYDVITMLKAYL
                     STMSPDKIILGVPYYGYNWLIDEQAPYANRVEGNDYIGYSESQPYETIVYDIKELNSL
                     ILWDNIGQTPYYYYRSPDSGALRVVHFENKDSLKMKYGLVKQYNLGGVGLWALGYDGQ
                     LPELWDLLGKEFGKK"
     gene            complement(10069..10145)
                     /locus_tag="PLF29_00915"
     tRNA            complement(10069..10145)
                     /locus_tag="PLF29_00915"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10109..10111),aa:Val,seq:cac)
     gene            10224..10748
                     /gene="lepB"
                     /locus_tag="PLF29_00920"
     CDS             10224..10748
                     /gene="lepB"
                     /locus_tag="PLF29_00920"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HXK52534.1"
                     /translation="MPNFRRAALHFLLDTIEILVIGTCLAAFSWLVIAEAVEVTGDSM
                     LPTLQNRERLIVEKVSIKLDKVGRGDIIVFKSPQDDKLLIKRVIALPNEYLEIISQDV
                     YVDGKMLPEPYVSNHENMSSMKVQLKDGEYFVMGDNRGVSNDSRRFGPIKQSSIFGHA
                     VLIISPVQEFRIIN"
     gene            complement(10945..11205)
                     /gene="rpmA"
                     /locus_tag="PLF29_00925"
     CDS             complement(10945..11205)
                     /gene="rpmA"
                     /locus_tag="PLF29_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017659733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HXK52535.1"
                     /translation="MAHKKAAGAKARQGSNTAGKRLGVKVFGGGVVKAGQIIVRQRGR
                     TFKPGANVGMGRDFTLFSKVSGIVKFVNDTRKKKRIDVVTEA"
     gene            complement(11218..11775)
                     /gene="rplU"
                     /locus_tag="PLF29_00930"
     CDS             complement(11218..11775)
                     /gene="rplU"
                     /locus_tag="PLF29_00930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HXK52536.1"
                     /translation="MKYAVIQLGGKQLRVQEGDVFEIERQNEINLEVLMYSEEGILLL
                     DAESLKGVTVKTTILEQKRDDKIRVARFKSKSRYRKVKGHKQPISVVKIDSISVGKKS
                     TEETKTKAENQIKPVSKVAKSTIEAGVKKSAEKEAKAPAKVAVKKVEKKSTPATKKAE
                     NKKAPTTTKKTVKKTTKESTKPKKN"
     gene            complement(11792..12094)
                     /locus_tag="PLF29_00935"
     CDS             complement(11792..12094)
                     /locus_tag="PLF29_00935"
                     /inference="COORDINATES: protein motif:HMM:NF024061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HXK52537.1"
                     /translation="MYFYCINCAKKVFFKAPGTKNRNHCPFCLCSMHVDNTIGDRKSE
                     CGGAMAAVAKIIKKDGEEVIVHKCNKCGLIRKNRVAGDDDIDLVENLMIVESLDHL"
     gene            complement(12096..13463)
                     /locus_tag="PLF29_00940"
     CDS             complement(12096..13463)
                     /locus_tag="PLF29_00940"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HXK52538.1"
                     /translation="MPDLILSVSNNILKASVLSKEGLLQSSVDVSPEIVDQDGSINNT
                     QELAQAIFKVVEGFTAVKKRHLQLVYLLQPSQSELFFVTSSKNQDSLDTFIIEEAKNK
                     LTEDIDSYYFAYKKIAPFVYQFIAVKKQLLDSILGLSLELKMPLKAVLPWVLLLPKFM
                     QSNDPAVFISTNGTSDEKMLALAELNGIYHVEEKYTFKDAAAMNAYIDELRVFKKASP
                     INRLYLVDGEDDSKLESSFEVSRLLELDEDFAQAKGYEVHLMAESMLKDNPELLQTQV
                     NALNLLPLPAVSRSLVPVYAGVAMSAAVVLLVVSGYVFLYRPRLEASRLAASNTDNAP
                     TVLSQETQTNDTTQGSADLEDEDAIEDLNKEDIVLRVENGAGIAGLASQTRATLEELG
                     YKVEGIGDSDKSGLETTTIQIKESKKAYASVLEEDLQDEFTVEILTDLPEDSEYDVLI
                     TLGSN"
     gene            complement(13527..>14641)
                     /locus_tag="PLF29_00945"
     CDS             complement(13527..>14641)
                     /locus_tag="PLF29_00945"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HXK52539.1"
                     /translation="SGMLLDEKGKKLSKSKKNYQPLDTVLDKYGGDVLRYFLLNSPIV
                     QGESPRFYEQVLIDARKEFFLPLWNCVKYFVTYANKAEFEPDLNVPKSDNVLDKWVLA
                     RLQETINVVVEKMDDYTVMEAARQLAPLVNDLSTWYVRRSRDRINSGDAESLHVLFFV
                     LSSLSKLIAPFVPFMAEEIYQTLNLPDYTEFGSVHFDFFPSYKELEQSEIEILQRMAN
                     TREVVSLALSVRVSEAIKIRQPLAGLYVTSESLNLFSDLIEDEVNVKVVHVGSEIPSQ
                     ISAMPFSESKEYKVYLDTTLTRELELEGAARDLIRKIQDMRKEENLDVSDRVKVFLMD
                     EADNAEILKMFGDYIKDKVGAEEIEFSTEYRVQNLA"
BASE COUNT         4659 a         3128 c         2670 g         4184 t
ORIGIN      
        1 gtgtgcaata accccctcaa gctgtctttt ttccagcctg tcaattatac ctgaggtgaa
       61 acatattaca cccttatcag ggcttcgccc tgtagcaaaa gcgttgagag cagtgtcgtt
      121 gattacataa agcttaggtt ttggtaagcc ggaggctata cacatgttct ctaccagact
      181 atgaacgtac tggctgtctt caggttttat ctcccttgct cctgaaattg cgagtactag
      241 tgaatcagat ttatagtacc caacaaagcc tgaaacaaaa gaaaacaaaa aagcaaaaac
      301 cagcatggaa gagtcaccga agtacgcaag gcttgcaaaa taaaagaccg ctgtaacaaa
      361 aaatataaaa ccaataaaaa tcaccgcagt ttcgcgttta ttcttatcta cacttgagta
      421 tacgcttgac attgaagaat tgccaaaaaa ctaaaaggct acttttacag cctctttttc
      481 ctgttccgtg gcctcaaaat actctctttt tttaacgtta aagatacttg caacaagatt
      541 tgacgggaac atatcgattt tttgattcaa atcccttact gtagcgttat agaattgtcg
      601 agaataagat accttatctt ctgtgtcaga tagctctttc tgaagattta caaaaccttc
      661 ttgagctttc aattcgggat atgcctcggc aaccgcaaaa agcgacttaa gggtttggct
      721 gagctgatta tctgcagccc ctacttctgc aggtgttacg gctgacataa gactggccct
      781 ggccttggtt acattttcaa atacatcttt ttcgtgcgag gcgtagccct taactgtttc
      841 gactaaatta ggtattaaat ctactcttcg cttaagctgt acatctatct ggctccaaga
      901 ctcctccacg cgggtgcgta atttgacaaa catgttgtat aaagcaacaa catatagaac
      961 aatagctaac ggtatcagta aaagaatgct taaaatagac acatttcctc ctaaatttaa
     1021 atatttctaa tgtttaattc tctcaaaaat tccttcaacg gtaaagtgtt tagaatcttt
     1081 tctttcgaac accacccacg cctagctaca taaacaccgt acctcataaa atttagcccg
     1141 gccgtgctgt gcgcatctgt gtttatcata atcttcactc ccattgcgca agcttctcga
     1201 accaaatcat ctgctaaatc aagcctgtta ggctgagcat taatctctat aaccttatcg
     1261 tgttctattg ctgatttaaa aacagctgtc cagttaacgt cacaagcagg ccgttcattc
     1321 aaaagtcttc ctgaaggatg gccaataatg tttataaggg ggtgttcaat ggcgtttatt
     1381 aatctctggg tgatagtatc tcgatcttga tcaaaagcgg tgtggataga gccaatggca
     1441 aagtttagct ttgacagcga ctcgtcaggc aacgaaatgc taccattggc aagaatatta
     1501 acttcataac ctaaaagtac ttttagattt gtcgaactat ttatgtgttc aacacttttt
     1561 atatgagtat tagttatgtt cattacttct tttagtcctc tgttgctaac gctaggggca
     1621 tgatcagcaa ctccgaagta ctcataaccc aaggcatccg ctgcatgtac catttcctct
     1681 aaagtattcg ccccatctga aaaattcgta tgaacatgaa gctccccctt aatgtcctct
     1741 agattcacca gttcggggag agcttttttt tgagccaaaa caacctcgtc tttcccttgt
     1801 ctaagctccg gctcaatgta tgacaaccct aaataggaat acagatcttc ttccctggag
     1861 aaagttttca agctcttacc atgtttaatt ccatattccg agagagactt atcttgctct
     1921 agagcatgag ttcgcaaaac aatgttatgg gcctttgagc ctgtaaaata ttgcatcata
     1981 cttccaagct cttcgtgcga aactgtgcga atatcaatct gcatgttgtt tgttagattt
     2041 acactcatct tcttctcgcc ttttacaaga acatcctcga tttcttcata tttttcgaaa
     2101 tggtcaaaga cctcatcagg tttggctgta acaatcgcta tatctagatc tccaacggtt
     2161 gcttcacgtc tcctataaga tcccaaaaac tccagtgcag aaacttccgg gcacaatcta
     2221 agataatatt ccagtctttt agccacctgc tccgccacta caaacaacga acgttctttc
     2281 tcctttttat gaagcttcat ttctgtaatc acagctaata gctctgcctc tgatctctca
     2341 ccaaagccag gtaaatctct aattttacct gcctctgcta tcttcttaat ttcctcaacg
     2401 gcggtacttt cgtcatctaa tgcaaaggca gaagccagct taaaggcttt tttagaacct
     2461 attcctcgga tacctattaa cgcaaacatt ccggcgggta aatcagcttt taatctattg
     2521 aactcgagta cttcaccggt cgtgaatagc tctttgaata cttgagttag ccccccgcca
     2581 acccccggaa tttcggaaag cctgccttgc tgccacagcg tgtatacact agtcgaaagg
     2641 gattctattg atgcaatggc gttctgatag gcccttatgc gaaatttgtt gtagtcttta
     2701 acctccatcg cagcaagcat ctgcttcaaa atatgcacta attcagggtt tttaagcatg
     2761 taagtcgagc tcatagagta ataataacat cgaaagtttg cgtaaagaaa ggttgaagaa
     2821 agatgtttac gttacaagta gactttactt ggatatttta tagattttaa tatttagaag
     2881 actaacgaag actcggcgga ctaacgtccc cggtctcttc tttctttgcg gttgaaagct
     2941 cacgccctca tccccgggct aaagcacggg gtcttcccgc cgcagaaatg aagattagag
     3001 gtcgtaacac tcattgaccc atgttcttgt atttgatact atgctctcaa ccaagttttg
     3061 gggccatagc tcagttggtt agagcgctgc cctgtcacgg cagaggtcgc gggttcgagt
     3121 cccgttggtc ccgccataat ttcagacaat ttgctgattt ttttggaaac ttttctttct
     3181 actgcctagt atttagttac atataaggca gcgatgaaca cagaaacaaa aaaagacttc
     3241 ctcgcacttg tcatagcttt aatttttgtc ggatcagtga gctattttgg ctactcttac
     3301 tttaatcaag gcgcacagca aaataaaggg gagatcggat cttcggcatc aacaaaaaga
     3361 actgaaaccg aagagacaga atcatatggc tctaagcttt ataaaattaa aacaaaagta
     3421 aacgagctta tagagggctc agaagaaatt caagaaagag attcttctga aacaacgacc
     3481 aattataaag aagaagccaa ctacagcgag aaaaacgaag attcagctca accaggagac
     3541 ggggcagaag aaaaatacac cacccctcaa gtagcgcagc cacattcaaa cgaatggact
     3601 gcgaccgact atgtaaaagg ggacattaaa gttcaaaata ataaatatga ggttaaaagt
     3661 ggcgacacac tttgggaaat agctgaagca gtctacggta acggttcgga atgggttaat
     3721 atactttccg taaatgccga caacatagga tatcttaaca acggacaaca agccttaatt
     3781 tttccggggc aaattattac aattccagta aacgccggca ctgcgatata ggcaaagcac
     3841 caataaaaag tgcgaacgtt aaataaatgt tatcgggaag acactcaaat ctttgacaaa
     3901 aagagggcct gacaattgcc tatctagatc tttattattt caagatacgc atttatcaac
     3961 caccactatc taagcggctg tttgctgtaa ttgtaaagcc tggcatgttg cataagctac
     4021 atctactaga tcatgcaagt agttttaagt aacgaaatta taaagtataa ttttgatggt
     4081 gccaccctag ctcaaatggt agagcaggcg tatcgtaaac gtcaggttat gggttcgatt
     4141 cccgtgggtg gctccaaaca gacctaacag aaagaaaata tggcacaaaa aagacattat
     4201 catgaagaaa tactaattaa gagacttaaa agaactatat tggctgttat aggtataccg
     4261 gtactggtaa tagccataat ggcttttttc ggccccaccg tggcttcaat atttggcttc
     4321 gtatcaatag acagatggac aaaagctcct ggtgatacga tcgcacccac cgttccgggc
     4381 ttcagaagcc ctcccggaag cgttaaggat acaaaaatta ctctgaacgg gttcggagaa
     4441 ccgggatcag taataaaatt gttcattaat ggcccggaga aacaatctac agtagtagga
     4501 aacgacggaa ccttcacttt tcaggacgtt gaacttataa acggaaaaaa cacattgttt
     4561 ttgaaggcac aggatcaggc aggaaacgag agcgaagcgt cagaaagagt tgagatcact
     4621 gtagatacaa tcgagcctaa aatagaaata accgctcctg aaaaagataa aacggtaaaa
     4681 aacctaaatg agagagtact aattcagggc aaaattagcg aaaaagccac tattagaata
     4741 aacgacaggc ttgcagtaca aaagcccgac ctcacttttg aattcattct aggggtaaag
     4801 cagggtgagg tagaagttac ggtcgaggcg gttgacgaag cagggaataa aaaggaagaa
     4861 aagctaaagt tcacctatct caaacaaagc gagtagcaaa aagaacaaat acataatctg
     4921 gggataaaat aaagaattta taaactgaga actcaccccc aagcctacaa tcagggatat
     4981 atagtacaag cttttttcgg ctgggaaggc agaggggcct ttggcaagtt ttgccaaagg
     5041 ccccaaaaaa ccaatcaaaa aaagtccaaa cccgacaact cctgttgtag caagaacaaa
     5101 tataaaactc gaatctatac cggcccctga atttccaccg tcctcaatag aataatcaat
     5161 aaaagacaaa aggttgttct ctttttgcgc gtatctaagc gtattaaaac cgacgccact
     5221 taccgggtta cttttaaata agtcgagccc gttttcccac gatcgccatc taaagctagc
     5281 agagtcggca ggatttgtta cgcctaaaat acgcccctga acccttggaa cagtaaagaa
     5341 caccaagctg catataaaaa tagacacaag aagcattcta ggctttctaa agaccccgta
     5401 caccaaccca atacatgcta aaaacaacca gccgcttctt gagaaagtaa gaaatattgc
     5461 aatcagacat aataaaaaaa ataaacctaa tatatttctg gagaaaaagc ttcttatgcg
     5521 aataagaacg agaagaataa tgcaaagata tgtagcggca aaatttggat cgaaaaatgt
     5581 agatgctagc ctaaacttat ggggatccca ccctaaagat ctttccaaca caccaaagtc
     5641 gggtaaaaaa tataattgaa taaaacctaa tatgatgaac aacaggcctg aaaaaactat
     5701 agctttctcg agccttatgt ggcagactag gccgttttta acagaggacg caacgcagaa
     5761 accaaataac aagtatgagc taaaacgtac aaaatagaga aaagactcag aaaattcttc
     5821 aggtatcatt gaaacgggcg taaccaaaag cgacaccaag gcccatgtag aaaatactgc
     5881 aaaaggaaat aatacagact caacatacag tttcttaccg cttaataata gataaaaaac
     5941 gcagtatagg gccgaaaatg cgatataaat gtcaaaaaag taaattccgg gccctccaaa
     6001 atagctcaac ttggctaatt gtcccaacat gagaataaaa ggagaaaggt aaattatact
     6061 agcgagcaac atatgttttc caggacttac gggcaaaaat aaccaacaat ataacaatga
     6121 agagctcgga aagccaagtt aaaacggaag aagcataaaa cgagtacatc ggaattgtaa
     6181 taagattcaa ggtcacgtta aaaactgccc ccaagagata cactgcgggg aggtattttt
     6241 gcttacccag cgaaactata aaccaagaga taggttgcga aatatagaat atcggcaaac
     6301 ctataagcag cagcctcaag actccggctg actgtagaaa ttcttgtccg cccaataaat
     6361 ttattacata acccgacaaa cctgccccaa cagccgcacc tgcaaagcct aaaaggaaca
     6421 gaaagccaat tgttttataa aacatcaaca aaaagtcttc ttttcctgtt tccagttttt
     6481 gaataaaaat agggtataca gcattcatga aaaaagtcgg taaaactaaa gcgacttcaa
     6541 agattttata cggcagacca tagaccccca cagcctcggt ttgagataat tttataaaag
     6601 cgggcacggg tagaacagac ataagaattg catcagactt aaagttaatt tgactaaaaa
     6661 caaacatcag tcctaaaggc actgcctgga tcagaagagg tttgtaaatc tctttttgta
     6721 atcttattcg cgctctaatg aaaaaaaact tctttaataa aaaatactga gccaagaatg
     6781 ttattgatcc gccaacaaca taagcaaagc ttacccacat aacgttctgc ttgaaatgta
     6841 taagaactaa cgcggtaatt aatatagccc cataaccgag aatatatgag attgtcgaca
     6901 aatcatatct taaatatttt tgaaaaataa tatttgcgga agcataaagc gcctgcgtaa
     6961 ctacaagaca cagattcaaa ataataccaa ttttcagcaa cctattatag ggaaaaaagc
     7021 ttaacacacc aagcgccacc aagattatca gaaaagaaat aaccagcctc aatcctatta
     7081 catttgctaa ataagtctca agctgagatt tatctttttt cacatctcta ataactacag
     7141 cgttaaaacc gaaatcaaca attatgaaaa aaagtgcagg ccaagactgc atgaggttaa
     7201 actctccata gccttccctg ccgtataatc tagaaaccaa aacagtaaca aaaactgtaa
     7261 tacccatact taaaaactta ccgataatct gatacaaagc atttgaagct actacaccct
     7321 ttgtgctgct tgggcctgtt aactttaata atttagttac aatcatttga ctctattcta
     7381 ccagtaaata atgtctccgg cgtaccggca acctttttca tgctctttac ctcaaaaaat
     7441 tgctcaaaag gctctttatc tacatcgacc caagcaaagt gatcgaatgg cggcggatac
     7501 caaattgtaa atggtatgac ggccttaatc ctcttatcct taagccaaac ctgttcgtag
     7561 gcaattttaa aattctctcc aactcttttt gatgtgtaat atgaaggatt ataggacagg
     7621 ccttctgcat gagcccaccc ggtttctgta ataaatatag gatattcttt tgatataccg
     7681 agtacggaag acagataaga cagctcaaat tcataggccc taacactatt tctgcccgaa
     7741 ccataaggag agcctgaaaa gttaggctga gggtaagaat gagaagccca tccatcgagc
     7801 ttattaaaaa cctccgggcg cgaagagtac atttgattaa taaaatccaa tgcgtcaatt
     7861 gtcccatttc cacccgaagc acttatattt aacgctccat ttaacataaa gtagtttgaa
     7921 tttatgtctt taaagatgtc tatagttgta tccagaatct cagcatattg tttgggatcg
     7981 gctttaccat accaaaaatt cgaatcattg ggctcattgt aaaccgaaat atagcgatcc
     8041 tttacaggcc aatcaaaaaa attcaaaaac tccgcacctc ttttagtatc cctgggaatc
     8101 tcatccaaat ccaaggcatt aagctgaatt attggaatta agtgcttttc tgcaagcacc
     8161 tcaaaaactt tacgccactt ttcaaaatca cggtctgtac taatattgaa ggggattaaa
     8221 acgtaccccc agtctccccc attagagttt accagttctg ccgctaaatc aaggaattcg
     8281 gtgtcttctg cataaacata gataccgtaa atattattgt tgtcgttacc tattgcatct
     8341 ttgtagtact ggtaattaaa ctctaaagag gtgcatccgg catcttgctt aggcagttta
     8401 gtattaaata tgaatacttc cggcagagta tgcgaaacaa acgcctcacg cagaatataa
     8461 ataaaaatcg taaacagtaa tgagattaaa ctaaataaga ttacatacaa tacttgggtt
     8521 agaatgtgtc tcacattcat attctacgag tgtaagcaat ttctcgcaaa cgttacatta
     8581 aaaggcatag acaaacacca tactaaacaa gtgttattta aaacttatca ctgtataatg
     8641 aattagtgga tctgctcaaa aaacttatca ttattctata cgccacctca ttaggtttga
     8701 tgacggtaaa ttttattttt gtaaaaacgc caaaaacgta taacataaaa tccccggttc
     8761 acggtagttt ttcggtcctt gctgcggtcg atcaacaaag agaaaattac attgtctacg
     8821 gctttttacc ttactggaaa cttgataacg caaaattcat acaaacagat aagctaactg
     8881 acattgccta ctttgggctt aagatagaag gaaacggcga aataagaagg tttgaagagc
     8941 ccggagtact agaaccaggc tacaacaact ggaaaaataa ccctgaactt tcaaacctca
     9001 tttctcgaag caaaaaagcc ggagttagat ttgctttaac cataatatct cacgaagacg
     9061 aggtttcaga tgagttttta aattgcacat cctgttggca aacactaata aatgagatag
     9121 ggattgaact tgaagaaaaa aatataaccg acgtcaatct cgattttgag tactttggat
     9181 acacagattt ggaaacagcc ttaaaatact ctgcctttgc taaacagcta aacgacacgc
     9241 tcgatgctcg ttacaataac aactcgaagg ttatcgtctc aacatttgcc gattctgttg
     9301 ttaaaccaag agtcacacac attgacacgt tagcaaaata tacagacggg ctttttgtta
     9361 tggcatacga ctttcacagg cccgcctcag ctactgcagg accaatcgcg cctttgggcg
     9421 gaatgggcga taaaagctct tacgacgtga tcacaatgct caaagcgtac ctatccacta
     9481 tgtcgcccga taaaataatt ttaggcgtgc cgtactacgg ctacaactgg ctaatcgacg
     9541 aacaagcacc ttatgcaaac agggttgaag gtaacgacta cataggatac tccgaatctc
     9601 agccttacga aacaattgta tacgatatta aggagctaaa ttctttaatt ttatgggata
     9661 acatagggca aactccatat tattactatc gatctccgga ctcaggagct ttgagagtcg
     9721 ttcactttga aaataaagac tctttaaaaa tgaagtatgg gcttgttaag caatacaacc
     9781 tgggcggtgt cggattatgg gccttaggtt acgatggcca gctccctgaa ctatgggact
     9841 tactggggaa agagtttggg aaaaaatgaa gactccgtgg actgaaatcc cacggctctt
     9901 ctttctttgc agttgagagc tcacgctttc atcttcgagc taaagcacag gaccttcccg
     9961 atgcagaaat aaatagcgtg taggttcgaa accactaact aagttgaaat atctttatct
    10021 atgagaggct tcttcgcaaa atatgtgaca ggttaggtgt ttttattatg gtggtcggaa
    10081 caggactcga acctgtgacc cctgcaatgt gaatgcagtg ctctaaccaa ctgagctacc
    10141 cgaccttaca aatatcagac ccggagttgt tgccgaggcg agttaattat acaacatgag
    10201 tgctttttgt tactatatta tgtatgccaa attttcgaag agccgcactc cattttttac
    10261 tcgatacaat tgaaatatta gttataggta cttgccttgc cgctttctct tggcttgtaa
    10321 ttgcagaggc tgtagaggtt acaggagatt caatgctccc aacactacag aatagagaga
    10381 gattgattgt agaaaaggtt tcgataaaac tagataaggt gggacgtggc gatataattg
    10441 tctttaaatc cccccaggat gataaattat taataaaaag agtaatcgca ttaccaaacg
    10501 aatacctgga aattatttca caagacgttt atgtggacgg gaagatgctt ccagaacctt
    10561 atgtctcaaa tcacgaaaac atgtcatcaa tgaaagttca attaaaagat ggggaatatt
    10621 ttgtaatggg agacaacaga ggcgtgagta acgactctag aaggttcggc cccattaaac
    10681 aaagctcaat attcggccac gccgtactaa taatcagccc cgtgcaagaa tttagaataa
    10741 ttaactaaaa aaacaacccc agaatccctc tacaatcaac tgccaccagc ttgtgcaggt
    10801 ggtttgctgt aatttctttg cactgcaaag cccaacatgt tgggtaatct gcatctacta
    10861 gcctgcgcaa gtagatttag gtaacgaaat tataaaatga aggttcagga aaattgcaaa
    10921 ctttttacga tgagcttgaa tctactatgc ttcggttact acgtcgattc tcttcttttt
    10981 tcgagtatcg ttcacgaatt ttacaatacc actaactttt gagaataagg tgaagtctct
    11041 ccccatgccg acattggccc cgggtttaaa ggttctgcct ctttgtctca cgataatctg
    11101 ccccgcctta accacacctc cgccaaaaac tttaactcca agtctttttc ctgcggtatt
    11161 acttccttgt cttgcttttg cacctgctgc ttttttatgt gccatagttt ctccaaatta
    11221 atttttttta ggttttgtgc tttctttagt cgtctttttt actgtttttt tagtagttgt
    11281 tggtgctttc ttattttccg cttttttagt agcaggggta gactttttct ctactttttt
    11341 tactgctact tttgcaggag cttttgcttc tttttcagca ctttttttaa ctccagcttc
    11401 aattgtagac ttagcaactt tcgaaacagg ttttatctgg ttctctgctt ttgtctttgt
    11461 ttcttcagta gattttttac caacacttat agaatctatt ttaactaccg aaatcggttg
    11521 tttatgtccc ttaacttttc tgtatcggga ttttgattta aaacgtgcaa ctcttatttt
    11581 gtcgtctctt ttttgctcaa ggatagttgt tttaacagta acgcccttta aagactctgc
    11641 atccaaaagt aaaattccct cttcagaata cattagaact tcgagattaa tctcgttttg
    11701 acgctctatc tcaaagacat cgccttcttg aactcttagt tgttttccac ctaattggat
    11761 tactgcatat ttcataacat tgtgaataat actacaaatg gtccaatgat tcaacaatca
    11821 taagattctc gactaaatca atatcgtcat ctcctgctac acggtttttt ctaattaaac
    11881 cgcacttgtt gcatttgtgt actattactt cctccccgtc tttctttata attttcgcga
    11941 cagccgccat ggcgccgccg cattctgatt ttctatcgcc tattgtatta tctacgtgca
    12001 tagaacacaa gcaaaaagga cagtggtttc tattttttgt tcccggggct ttaaaaaaaa
    12061 cctttttcgc acagtttatg caataaaaat acatattagt ttgagccaag tgttataaga
    12121 acgtcgtatt cggaatcttc aggcagatcg gttaagattt ctactgtaaa ctcatcttgc
    12181 aagtcttctt ccagaaccga tgcgtaggct tttttgcttt ctttaatctg tatagtggta
    12241 gtttccagcc cacttttatc ggaatctcct ataccttcaa ctttataacc cagctcctcc
    12301 agagtcgcgc gcgtctgaga tgccaaacct gctattcctg cgccgttttc aaccctaagc
    12361 actatatcct ccttatttag atcctcaatc gcatcttcat cttctaaatc agcgctgcct
    12421 tgggtagtgt cgttcgtttg tgtctcttgg ctaagtactg taggggcatt gtcggtgttt
    12481 gatgcagcaa gacggcttgc ttccaaacgt ggtcggtaca agaacacata tccgcttaca
    12541 accaatagaa ccacagccgc cgacatagca actcccgcat acacgggaac caaacttctg
    12601 gagacagccg gcaggggcaa tagatttaaa gcatttactt gtgtttggag cagttcaggg
    12661 ttgtctttta gcatcgattc ggccatcaaa tgtacttcat aacccttggc ctgggcaaaa
    12721 tcttcgtcaa gctcaagcag tctcgaaact tcgaaagaag actcaagctt gctgtcgtct
    12781 tctccatcaa ccagataaag ccggtttatt ggggaggctt ttttaaatac acgaagttca
    12841 tctatgtagg cgttcattgc agcagcatct ttgaaagtat atttttcctc aacatggtag
    12901 attccgttta gttccgcaag agccagcatt ttttcgtcgg acgtgccgtt tgtggatata
    12961 aacactgcgg ggtcgttgct ttgcataaac ttaggcagaa gcaaaaccca aggcagaacg
    13021 gctttcagag gcattttaag ttctagagat aggcctagta tagagtctag gagctgtttt
    13081 ttgacagcaa tgaactggta tacaaaaggg gctatttttt tataagcaaa ataatagcta
    13141 tcaatgtctt cggttagctt gtttttagct tcctcgatga taaaagtatc gagcgagtcc
    13201 tgatttttac tagatgttac aaaaaacagc tccgattgag aaggttgcaa caaatacact
    13261 agttgcaaat gccttttttt gacagctgta aaaccttcaa ctaccttaaa tattgcttga
    13321 gcaagctcct gcgtgttatt aatcgatccg tcttgatcaa caatttcagg cgaaacatca
    13381 acagaagact gtaaaagtcc ttctttagat aatacgctag cttttaaaat attattactt
    13441 actgataaaa ttaaatcggg catacataaa atataccata aaaatggttc ctaaaactac
    13501 gtattgcact aatttaagaa atcaaactaa gccaaatttt gaaccctata ttcagtagaa
    13561 aattcaattt cttcagcccc gactttatct ttaatgtaat caccaaacat ctttagtatc
    13621 tcggcattgt cagcctcgtc cattaaaaat actttaaccc tgtccgagac gtctagattt
    13681 tcttcttttc gcatatcttg tatttttctt attaagtcgc gcgccgctcc ttcaagctct
    13741 agctcgcgag taagtgttgt atcaagataa actttgtact cttttgactc agaaaaaggc
    13801 attgcagata tttgagaagg tatttccgaa ccgacatgta caaccttaac attaacctca
    13861 tcctctatta aatccgaaaa taagttcaaa gattcacttg taacataaag tcctgctaaa
    13921 ggctgcctta tctttattgc ttccgaaact cttacagaaa gcgccagcga aacaacctca
    13981 cgcgtattag ccattctctg aagtatctca atctcggatt gctccagctc tttgtaagaa
    14041 gggaaaaaat caaaatgcac ggaaccgaat tctgtatagt cgggtaaatt aagggtctgg
    14101 taaatctcct ctgccatgaa aggcacaaag ggtgcaatta gcttacttaa ggagctcaaa
    14161 acaaaaaata atacatgtag ggattcagca tctcctgaat ttatgcggtc tctagaacgt
    14221 cttacatacc aggtagataa atcattgacg aggggggcaa gctgccgtgc cgcctccata
    14281 actgtatagt catccatttt ctccacaaca acattgatcg tctcctgcaa tcttgccaac
    14341 acccatttat ccaaaacatt atcgcttttc ggaacattta aatcaggttc gaattcggcc
    14401 ttattcgcat atgttacaaa atattttacg caattccaca acggtaaaaa aaactctttg
    14461 cgggcgtcga tcaaaacctg ctcataaaat cttggacttt ctccctgcac aataggcgaa
    14521 ttcaataaaa agtatcgtaa aacatctccg ccgtacttgt ctaatactgt atcaagcggc
    14581 tgataatttt ttttgctttt gcttaacttc ttaccctttt cgtcaagcaa catacctgaa
    14641 a
//
LOCUS       DAOSWN010000008        14225 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_2704_length_14225_cov_5.253423, whole genome shotgun sequence.
ACCESSION   DAOSWN010000008 DAOSWN010000000
VERSION     DAOSWN010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14225
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2704_length_14225_cov_5.253423"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /locus_tag="PLF29_00950"
     CDS             <1..1120
                     /locus_tag="PLF29_00950"
                     /inference="COORDINATES: protein motif:HMM:NF018406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1189 family protein"
                     /protein_id="HXK52540.1"
                     /translation="AANKGLAKLAIPLPTHSAVAVDTFPPALDLRAPNQKEKPSEVTN
                     IRVNTNLFCKNLSAAIHNYYTSKLVRGVKILRNNFYVNIVSMRPKTFVYTFIKSIFNL
                     QYYNDIMKAKVSFSIRYYLMLTLLLSLGYAVVTTTNTGPVVIEKYYEVRNEALKYYPD
                     DLSIIGEDGVVTTNQGDPYFFPLPKEIELGDGEMLKNAIVFSKQGTIEDIFNYESFAL
                     VNSKNIIYRSSPGTNNKLEIEPTKNLGNFTFTKADLEKAAQYVDTYIKYTYAIIGVSV
                     FIAFALVMLLWIMTGNVILSAIMTVASKLMNKKLPFEKAFQIALHTATLPLIVGALID
                     AFGIWMPISFWFSGLHFLLALIIILNLKEEAQTSPAQA"
     gene            1299..1373
                     /locus_tag="PLF29_00955"
     tRNA            1299..1373
                     /locus_tag="PLF29_00955"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1331..1333,aa:Asn,seq:gtt)
     gene            2581..4419
                     /locus_tag="PLF29_00960"
     CDS             2581..4419
                     /locus_tag="PLF29_00960"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXK52541.1"
                     /translation="MLYWSIKIPIGVASIAFLVSIGYGLQDLVISRVARLDEMQQAEI
                     YSPPASNTKINDESIAKIANVQNVEKVLPLITLVAKVNYNNSISDMAVYAVTSDYLEQ
                     SAIKPVKGKIFDNDLTSFNLTEEDENKEEVAGASSSRQGGEQGGEISASQNNNLNEGL
                     TNIEVGKRITEAEISIFPETWVRVREKPSLDSKILGYTKRPEGKQTGYEVWGNSYPDN
                     INGTIQAADGKVYGKWVESKVFLWEEKNNGYIQVIDEQGSQVQKTGFMAEVNLGLQRY
                     TLERQPEVLAEADTRAVTDSTETQTDSQITTGTGQAAAEDATTYEDLKFDGTDWILIE
                     EGNKTEQIERVQVSEKATREAVANTAMLDVLGLTESEAIGKEIEVSFIVTASLLEPGA
                     AKIESVPTKYKIIGVVPQKDAPYFYVPFIDIRSLGVMNYSQAKIIVQDQQSLTDVRKK
                     IEAMGFETTSVVDTVEQINTLFATLRKMLALAGTVALSVAALGMFNTLTVSLLERTRE
                     IGLMKAMGMKSFEVKELFLTESMIMGVFGGIIGVSLGALAGELLGFIISIFSISKGLG
                     YIDVASMPVGFTLFVLSLSLIVGTLTGIYPARRATNISALNAMRYE"
     gene            complement(4455..5024)
                     /locus_tag="PLF29_00965"
     CDS             complement(4455..5024)
                     /locus_tag="PLF29_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52542.1"
                     /translation="MKKIILVGAVLVAVSFGLIVLSKTSQTPKNNTSVTIEEQQLGSQ
                     KYVAKRPMDGQDSFGLSVCEEVPSELVSDIIGIPIQEVSDSSTSTGTGCTYFTNKARY
                     EHILVQVSYLSADTQKEGHKIAGRSIETDSSIPMEHFIAMQDDGNINAIYLVMTPNKF
                     VRVDRTPNTADNEQLKELARKVAIIILGE"
     gene            complement(5072..6439)
                     /locus_tag="PLF29_00970"
     CDS             complement(5072..6439)
                     /locus_tag="PLF29_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52543.1"
                     /translation="MAEKEDIIVPENEGPNKNLEAPLGANSESSAMSPSPDSEAHSLQ
                     GEGGVFPETSLPNERFDPLTSRQETASENSIPPITRDGFSNGSSGPVENRNNFSPNAE
                     FSAKRPQYPETEPHEKAASNIEPGVDNNNFENAPENNVGVEDNKEVGAEETQSAPEFE
                     LSPEATEGKDELEGEQGNTLAEEEVHREPLLTSEDTYAGNFEQEPGPKEKNEVELGVY
                     KEGFSIKKAFTIAGAVLLALLMTTAGFLSAKALIPYWQERSDSKKQQTSEMPVMSEEQ
                     NLGGENGDKDRKSLNETEPKRSVFSSLIGEEDVVIYEIDFSNMPENGLLCTNNKSFEY
                     HEAGEYFYYVSSIGGNINSGPTRNILHVNDDYFIWEEFETLPATGSYVWKLTSEEYTA
                     ETQSQYSSVASIVDAEIAAIKDQENLCEDWVADENMFTPSQYVLINYKNPTPEDLDQL
                     LLPKP"
     gene            complement(6606..8288)
                     /locus_tag="PLF29_00975"
     CDS             complement(6606..8288)
                     /locus_tag="PLF29_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52544.1"
                     /translation="MSDEQPNIIPIQPLDDGSSDNNNPGITRSGSTAKQENIPENVVD
                     QTVSGPEDSGPHPVAENISVEDQGAKLQEPPVVDSGTASDEGKDVPVSQVLEGGSALS
                     EEVSEDGTVAPHFLDTVSEEDPAAGDGGSSFQAQNPYVPADGEGAVGAPAATTTSKNK
                     PSRRIFSFMPNRTLYVFLLISFVVIGLGSSLVYLYNNSSLQPGAVEPLPTENPETPVV
                     IETPSKPDPDWKTYVNREYEFQIDYPKETLFKLPSDMAAQSVTYTITYAGEKQDEAIT
                     TATDISDGYLVKITMYKNVLNTDLAQIALNKRSGYLSLCPATAEYGDIKDAVISGEPA
                     KTFVVDNCNVNYVETFLIKGSNMYEIAQAYRGDVGFKQRYQAQTDLIREAFKFTNIIA
                     PEPKDTWTKIVHGNPELAVSYPSDWDDTCCNLRGPIAGEVTKYFVAVDPLSIGEDDQA
                     VFNGLAAYSLTLKNDETYESFMAEQKELLKEEYRVVVGIEPEVSDETFEVNGFVWTHL
                     TNYAWWGEIYFSYISDSNRVVVLVKSEEVPGKLDEVFSELLETVSYKFPQNQ"
     gene            complement(8300..10162)
                     /locus_tag="PLF29_00980"
     CDS             complement(8300..10162)
                     /locus_tag="PLF29_00980"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HXK52545.1"
                     /translation="MKDSPKKADPKEPKIKVTFSNSPAVWEFPSQWDLSVLMKSDEDA
                     AQDLERTEIAKSVEQFVSKHRESKDYLTSAPALKNALDEYEKLSRLISVGGRAGFYFY
                     LRSSLDSADNKIKAKLNSLNEFLTEKYNELEFFALTLGKIPEEKQKEFLSHHDLQDYK
                     HYLEQLFASAKHDLSEKEEQLLNLISKGAYGDWVDMVSTFYSLETVKVLNERHEKITI
                     SISEISKYLKSSDDKVQKYASKSLEQVLEKHIKTAEREINAILYYLKTLNKLRNFKRA
                     DERRLLSEDMDAATIDVLIEAVSANFGVSAQFYDLKAKLLGKKVLTYNQRNIEYGKLK
                     HEINVATAVEVVHDCFMSLDPIFGEYFESMLCGGNIDFFPKKGKSGGAFCVSITNDYP
                     AYILLNYNNEFRDVSTLAHEMGHAIHHFISSSAQNALNADYPMGIAEVASTFMEDFAN
                     ALLVKELTLEEQLIYKMLKLDDDISTVHRQIACYIFELALYDTYTKENYLPEELIGEI
                     FEEKMSLYLGKNSKGAGRHWAMWSHIRRPFYVYSYASGLLISKALQERVRLDPRFIDD
                     FKHILSLGSSVSPKDAFLTVGIDITKREVWDLGLAKISNDLAKATELAKTLGKI"
     gene            10217..11533
                     /locus_tag="PLF29_00985"
     CDS             10217..11533
                     /locus_tag="PLF29_00985"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HXK52546.1"
                     /translation="MSPLAQEPISIAEDNNLIINNTDEVRESLIDQLIYSAVFGSGKK
                     KGDARWIIKKIGQKLGVNFASINVFYKKRAKESSIKPFTVPAFNIRGLTYDTARTLFK
                     TAKKLNTDAFVIEIARSEMNYSKQNPEEFATCVIAAAIKENYTWPIYLQGDHFQAKAK
                     SPGIADVGEMEALKKLISEAVAEGFYNIDIDTSTLVDLGQKTIQKQQLPNILATAELT
                     DFIRTTYTSPNYPTISIGGEIGHIGGKNSTAEEFEIFMQGVESETQGAAGLSKISIQT
                     GTSHGGMVDDNGNILDVEVDFALLKNISLIAKNKFNMAGAVQHGASTLPDNLFRMFPK
                     NLTAEIHLATGTQNIIFDHPLFPETLKKGMDSWVLQNLQKERMPGWNNEQTIYKLRKK
                     TWGQFKKDLWEIDANTKKAIFADLQKRFIFLFAELGISDVKTQSQN"
     gene            11578..11964
                     /locus_tag="PLF29_00990"
     CDS             11578..11964
                     /locus_tag="PLF29_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003793932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HXK52547.1"
                     /translation="MTNTTKQKTEPEQISKTLTEVVQDEKSLAIIAHIGGIFFGIIAP
                     LIIWILQKDKPNASFSTHHAKEALNFQISVLLASAVAGLTTIVLIGFLFLPAVMVANV
                     IFCILAAIEANKGLPYNYPFSLRLVK"
     gene            12003..13688
                     /locus_tag="PLF29_00995"
     CDS             12003..13688
                     /locus_tag="PLF29_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52548.1"
                     /translation="MEENEAVIQNKDLPSEAEDQKNSRINKLKFRINGKKLLILGGAV
                     VLILLLISAVLVSNSKSKKVTPSTANESQETMKQKPIKSSEYNPSTEGEVVLPWTPNN
                     INTSHDTYYLFGEVKTDGDLFHVDLFTNGELVDDYTIGVNNYLLGLNFGLSNSGFVIE
                     KGRAHKNFYFPIRDLKVGENNVSIKLYTDDTKEKVLSEKSVIITRSDTTYNSEVTLYN
                     SPREVSAEEIFNKAGIDFENEKLWEYFHNQPSWFLDNKSNDFFYGKAGDWIDYKIEAM
                     GEIGIKNEKTSLYRFLYTHPGEGPGPSAYNNSIYFYIVNNQAYFVTNTLVSDEYFAPI
                     YLFYMQPSPYIFEDTVAELEEISINENNTLIPSATGVGVLRYCDPAKIEGEKIYTYQS
                     FSVYENFDVYDQYGNCKNFRLVPSYFRDDTYGTYPGASDWNDGVTPDFNKMTVNLDDG
                     KSLDSFYNPYWYWCARSRGYRTIDSSNYLEKVGKTSTGEDVYIYSTNMPFPKDSHLLP
                     SSTKEYPYYTASEENWQEFLNNQKETYPVLVIKNVFGEWVVYINADYLFAPGC"
     gene            complement(13685..13909)
                     /locus_tag="PLF29_01000"
     CDS             complement(13685..13909)
                     /locus_tag="PLF29_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52549.1"
                     /translation="MGNLDTFLRNMLNYAIVHSKEICGYDKPEIYLMGLSAGAGAISK
                     LASWYSVDWRYQPINLLSRHALFLTLQTLN"
BASE COUNT         4474 a         2849 c         2680 g         4222 t
ORIGIN      
        1 agccgccaac aaaggcttag caaaacttgc gattcccctg cctacacact ctgctgtagc
       61 agtagacaca tttccgcccg cattagacct tagggcacca aaccaaaaag aaaaacccag
      121 cgaagtaaca aatataaggg ttaatactaa cttgttttgt aaaaatttga gcgcagccat
      181 tcacaactat tatacatcaa agctcgtgcg tggagtaaaa atactaagaa ataattttta
      241 tgttaatatt gttagcatga gaccaaaaac ttttgtctac actttcataa aatcaatatt
      301 taatttgcaa tactataacg acataatgaa agcaaaagta tccttttcaa taagatacta
      361 cttaatgctt acccttttat tgtctttggg atatgctgta gttacaacta caaataccgg
      421 accggtagta attgaaaaat attacgaagt acgtaacgaa gctttaaaat actaccccga
      481 tgatttatct ataatagggg aagacggggt tgtcacgaca aatcaggggg atccttactt
      541 cttcccgctt cctaaagaaa tagaacttgg agacggcgag atgcttaaaa atgcgatagt
      601 tttttctaaa caaggaacaa tcgaagacat attcaactat gaaagcttcg cactggttaa
      661 cagtaaaaat ataatctacc gaagtagccc cggtacaaat aacaaacttg aaatagaacc
      721 cactaaaaac ttaggtaatt ttacatttac gaaggccgac ttagagaaag cggctcaata
      781 cgtagatacc tacataaaat acacctacgc aataattgga gtaagtgttt ttatagcatt
      841 cgctttagtt atgttgctat ggataatgac aggaaatgtt attttatctg caataatgac
      901 cgtggcaagc aaacttatga acaaaaaact accatttgaa aaagcctttc aaattgcact
      961 tcatacggcc acacttcctc ttattgtggg ggctcttatt gatgcctttg gaatatggat
     1021 gcctataagt ttctggtttt cgggactgca ttttctttta gcattgataa taatattgaa
     1081 tttaaaagaa gaggcacaaa cttcgcctgc gcaagcttaa aagaaataat cgaaaataag
     1141 agattgatat aggtgtttaa aggagaaaat ctcagatctt ttgtgaaaag ggcactgcat
     1201 tgaaaaacat aacaacagtg tgttaatatt ctgtacagtt ttgaacactc ggcccgtacg
     1261 taacaactac acaaggccgc aaaaaacttt acgagcggtc ccctgtagct caatggtaga
     1321 gcgcccggct gttaaccggt aggttggtgg ttcgagtcca cccgggggag ccaagtagaa
     1381 tcagtaggct cagaaacacc atatttagct gtgacagcaa ctaaatccac tggttctaac
     1441 cgtctatttt tcacatattc aggattttta ccctccttga atatcaagaa gggtttttcc
     1501 aagtcaatac acagttttct gttattaagc atgaggttct gaccaagtaa agataagatt
     1561 tctcttttaa cagtaagtcc gccattttca aatctctcac gaacacttgt agcaaagttg
     1621 aatacatctt caatcttttt gagatatgcc tctctatttt gttcatctgc acttagttca
     1681 tctttaagct cgtctcgttt agcttttact tcaactttaa tgtctaggta ctcttcgcta
     1741 gaaatctcat tattcatacg catggtcaac aaatctgcca actgcttctc tagtctttta
     1801 tattcttttt gttttgattc caagatttga agagaagttt cagattcggt tttactcttt
     1861 tcagtgaggt atttaaccgc ccaatcacga aactcaattg gtatttcgat tgatgacacc
     1921 aactctatta tctgctcttc taaatctaat aatgttatag atttttgctt gcatttaacg
     1981 tggggttttc ttttggaaca atgatagtaa tcatatatgc ctatattgcc cgttttagga
     2041 tagtgctttt ccttatgtgt tgccgttata gcacacccac attcagcaca atgtatcaga
     2101 cccgtataaa gatgtacgtg tgtctttggt tttcgtttaa gctttctgct caggatttct
     2161 tgcaccgagt taaattcctt tttggtcacc attttggggt gttcaccttt ccgtaactga
     2221 cccttatacc taaagtaacc ataataaaag gggttactga aaatgtcata taatcctgat
     2281 ttagatatag gtttgccacc agattttttt ctcttaatag agcggtaatt ccattggtta
     2341 ttagcaattt caagtatttc aggaactgta tatgctcctg taagcatcaa atcccacatt
     2401 ttccttacca aatcgaagcg ttcttggtct ttaatgatgg tgtttttacc tttttcctta
     2461 tacattgtgt ttagataacc cgcaggagca acagaaggta accacccttt ttcaactttg
     2521 gattctagac catctgcaac tctttcccct tttacatcat tctcaaattt agcattaagc
     2581 atgttgtatt ggagcattaa gattcccatt ggcgtggctt caatcgcctt tttagtatca
     2641 ataggatacg ggcttcaaga tttggtaatt tcacgagtag cgcgattaga cgaaatgcaa
     2701 caagctgaaa tctactcccc tccagcaagt aatacaaaaa taaacgacga gtcaatagca
     2761 aaaatagcaa atgtccaaaa tgtagaaaaa gttttacctc ttattactct agtagcgaaa
     2821 gttaactata ataactcaat ttccgacatg gcggtatacg ctgtaacaag cgactatctt
     2881 gaacaatctg caataaaacc tgttaaagga aaaatattcg acaacgattt aacaagcttt
     2941 aatctcactg aagaagatga aaataaagag gaagtggccg gggcatcgag ctcccgtcaa
     3001 ggcggtgaac agggtggaga aatttctgca tctcaaaaca ataatcttaa tgagggtctg
     3061 acgaatattg aagtcggaaa aagaattaca gaagcagaaa tttcaatatt tcctgaaact
     3121 tgggttagag tgagagaaaa accttcctta gacagcaaaa ttttaggcta cactaaaaga
     3181 cccgaaggaa agcaaacagg ctacgaggtc tggggaaact cctacccgga taatataaac
     3241 ggaacaatac aggctgctga cggcaaggta tacggcaaat gggttgaatc aaaagtattt
     3301 ttatgggaag aaaaaaataa cggctatatt caagttattg atgagcaagg aagtcaagta
     3361 caaaaaacag gatttatggc cgaagttaat ttaggattgc agcgttatac tctggaaaga
     3421 cagcccgaag tacttgcaga ggccgacaca agggccgtaa ctgattctac ggaaacacaa
     3481 acagattcgc aaataactac aggcaccggg caggctgcgg cagaagatgc aactacttac
     3541 gaggatttaa aatttgacgg aacagactgg atactaatag aagaagggaa taagactgaa
     3601 cagattgaga gagtacaagt ttccgaaaag gccacaagag aagcagtagc taatactgcg
     3661 atgttagatg ttttgggact aacagagtcc gaggcaatag gaaaagaaat agaagtttct
     3721 tttattgtta ccgcatcatt attagagccg ggggcggcta aaatagagtc tgtaccaact
     3781 aaatataaaa ttattggggt cgtaccccaa aaagatgcac catattttta tgtacctttt
     3841 atagatatta gaagcctggg agtaatgaat tattcacaag caaaaataat tgtgcaggat
     3901 cagcaaagcc ttacagatgt aaggaaaaag attgaggcga tgggttttga aaccacatca
     3961 gttgtagaca cagtagagca aataaataca ctttttgcga cacttagaaa aatgctggcg
     4021 cttgcaggaa ccgtggcact aagcgttgcc gcactgggga tgtttaatac tctaactgtt
     4081 tctcttttgg aaagaaccag ggaaatagga cttatgaaag caatgggaat gaaatctttt
     4141 gaagttaagg agctgttttt aacagaatct atgataatgg gagttttcgg aggaattata
     4201 ggcgtatcct taggcgcact cgccggagag ttacttgggt ttatcatttc aatattttct
     4261 atttcgaaag gtttggggta tattgatgta gcatcaatgc ccgtcggatt tacgctgttt
     4321 gtgctttccc tttcattaat agttgggact ctaacaggaa tctatcccgc ccgcagagcc
     4381 acaaatatat cagcacttaa cgcaatgagg tacgaatagt acctctctaa gaaaatttat
     4441 tggaggggct aaaattactc ccctaaaatt atgatggcaa ctttccgggc cagctctttc
     4501 aactgctcgt tgtctgcagt gtttggcgtt ctatcaacac gcacaaactt gttcggcgtc
     4561 attactaaat aaatagcgtt aatatttccg tcatcttgca ttgcaataaa atgttccata
     4621 ggaatggagg agtctgtttc gatagatctt ccagcaatct tgtgtccttc tttttgagtg
     4681 tctgcagaca agtaagaaac ttgaacgagg atgtgttcat aacgtgcttt gtttgtgaaa
     4741 tatgtgcaac ccgtgcctgt acttgtagag gaatcagaca cttcctgaat cggtatgcca
     4801 ataatatctg aaaccagttc tgaaggaact tcttcacaaa ccgaaagccc aaaagaatct
     4861 tggccatcca taggtctttt agctacgtat ttttgcgaac ctaattgctg ttcttcaatt
     4921 gttactgagg tattattttt tggggtttgg ctagttttag aaagtacaat aagaccgaaa
     4981 gatacagcaa ctaaaactgc ccccacgaga attatttttt tcatattctg attatatgcc
     5041 gttaatttaa aaaaatatag cgagaccgat tttagggctt tggaagtaat agttgatcta
     5101 gatcttcagg tgtcggattc ttgtagttaa ttagaacata ctgggaagga gtgaacatat
     5161 tttcgtccgc cacccaatct tcacataaat tttcttgatc tttaatagct gctatctctg
     5221 catcaacgat tgatgctaca gaagaatact gcgattgagt ttctgcggta tattcttcag
     5281 atgttaattt ccacacataa ctacctgttg caggaagagt ttcaaactcc tcccatataa
     5341 aatagtcatc atttacgtgc agaatatttc ttgtaggtcc ggagttaata ttaccgccaa
     5401 tcgaagagac gtaatagaaa tattcacctg cttcatggta ctcaaaactt ttattgttag
     5461 tacaaagaag gccattttcg ggcatattag aaaaatctat ttcatagatt acaacatcct
     5521 cttcaccaat gagacttgag aatacggatc tttttggttc tgtttcattc aagctttttc
     5581 gatccttgtc accattctct ccgcctaaat tctgctcctc actcataaca ggcatttcgg
     5641 aagtttgctg tttcttagaa tcagaccttt cttgccaata aggaataagg gcctttgcag
     5701 ataaaaatcc tgcagtagtc attaataaag ccaacaaaac tgcccccgca attgtaaatg
     5761 cttttttaat tgagaagcct tctttataaa ctccaagctc tacttcgttc ttttctttag
     5821 gcccaggctc ttgttcgaaa tttcctgcat aagtatcttc tgacgtaagc aaaggttcgc
     5881 gatgtacctc ttcttcagcg agcgtgtttc cctgctcccc ctcaagctcg tctttccctt
     5941 ctgttgcctc aggacttaac tcaaattcag gtgccgactg agtttcttct gcgccaacct
     6001 ccttattgtc ttctactccg acgttatttt caggggcatt ttcgaaatta ttattatcga
     6061 caccgggctc aatgtttgag gcagcttttt catgtggctc agtctcaggg tattgaggcc
     6121 gttttgcaga aaattctgcg tttggcgaaa aattgttgcg gttttctacg ggcccactac
     6181 taccgttgct gaagccatct cttgttattg gcggaataga gttctccgag gctgtttctt
     6241 gacgtgaagt taaggggtcg aatctctcgt ttggaagact tgtttcagga aaaactccgc
     6301 cctcaccttg taaagaatgt gcttctgaat ctggactggg cgacatagcc gagctctccg
     6361 aatttgctcc tagtggggct tcgagatttt tgtttgggcc ctcattttcc ggaactatga
     6421 tgtcttcttt ttcagccata aattaattct aacataaata agtgcattaa tttttataat
     6481 ttcgttacta aaactactta cataagctag tagatgtggt ttacccaaca tgttgggctt
     6541 tgcaacgcaa agaaggtacg gcaaaccact tgcgtaagct ggcagtgttt aatatcagca
     6601 gaaaattact gattctgagg aaacttgtaa ctaactgttt ccaataattc ggagaaaacc
     6661 tcatccagct ttcccggaac ttcttcactt tttacaagta ccacaactcg atttgaatcc
     6721 gaaatataac taaaataaat ctctccccac caagcataat tagttaaatg agtccagaca
     6781 aaaccgttta cttcaaaagt ctcatctgaa acttccggtt caattcctac cacaacccta
     6841 tactcctctt ttagaagttc tttttgttcg gccataaaac tttcgtacgt ctcatcgttt
     6901 tttaaggtaa gtgaataagc agcaaggccg ttgaaaacag cctggtcatc ttcgccaata
     6961 cttaatgggt ccaccgctac aaaatacttt gtaacctcgc ctgcaattgg ccccctaaga
     7021 ttacaacacg tatcgtccca atcagaagga tagctaacag caagttccgg attgccatgg
     7081 acaatctttg tccaggtatc ttttggctca ggagcaatta tgtttgtaaa tttaaaagcc
     7141 tcgcgaatta ggtccgtctg tgcctgatag cgctgtttaa aaccaacatc gcctctgtat
     7201 gcttgggcaa tttcatacat gtttgatccc tttattaaga aggtttcaac ataatttaca
     7261 ttacaattat ccacaacaaa agttttagcc ggttctccgg aaatcacagc atctttgata
     7321 tctccatatt cagcagttgc agggcacaaa cttagatagc ccgatcgctt attaagtgcg
     7381 atctgagcta aatcggtatt taaaacattt ttatacattg ttatttttac caaataacca
     7441 tcagagatat ccgtagccgt agttatagct tcgtcttgct tttctcccgc ataagttatc
     7501 gtgtaagtaa ctgattgtgc cgccatatca gaaggtaact taaatagagt ttctttgggg
     7561 taatctattt gaaattcgta ttctctgttt acataggtct tccagtcagg atcgggtttg
     7621 gaaggagttt cgattacaac aggggtttca ggattttctg tcggcaaggg ctcgacggcc
     7681 cccggctgta gactggagtt gttatataga tatactagag acgaccctag accaataaca
     7741 acaaaggaaa ttagtagaaa tacgtataga gttcgatttg gcataaagct gaatattctt
     7801 cgggatggct tgttttttga tgtggttgtg gcggccggcg ccccaacagc cccctcccca
     7861 tctgcaggaa cgtatggatt ttgagcttga aaagaagagc ctccgtcgcc ggccgccgga
     7921 tcttcttcac ttaccgtatc taaaaaatgt ggggcaacag taccatcctc agaaacctct
     7981 tcagacaaag cgctgccccc ttccaaaacc tgagaaacag gaacatcctt accctcgtca
     8041 ctagcagtac cgctgtctac tacaggcggc tcttgcaatt tcgcaccttg atcttcaaca
     8101 gaaatatttt cagcaacagg gtgcgggcca gaatcttctg gtccggaaac cgtttggtca
     8161 acaacgtttt ctgggatatt ttcttgcttt gccgtactac ccgaacgggt aattccagga
     8221 ttattgttat ccgagctacc atcatcaaga ggctggatcg gtattatatt gggctgttca
     8281 tcactcatgt agttatattc taaatctttc ctagagtttt tgctagttct gttgctttcg
     8341 ctaaatcatt tgagatcttc gccaaaccta aatcccaaac ctctctttta gtaatatcaa
     8401 tgccaacggt taaaaacgca tcttttggcg aaacagaact acctagagaa agaatatgtt
     8461 taaaatcatc tataaaccta gggtctagtc gaaccctttc ttgaagagct ttggaaatta
     8521 acaaaccact tgcatatgaa taaacgtaaa aaggtcttct aatatgtgac cacattgccc
     8581 agtgcctgcc tgctcctttt gaattcttgc ccagatacaa actcatcttt tcttcaaata
     8641 tttcccctat gagctcttct ggcaggtagt tttcttttgt gtatgtgtcg taaagcgcaa
     8701 gttcaaaaat ataacaggct atctgtctgt gaacagtgga tatatcgtca tctaatttca
     8761 gcattttata aatcagttgc tcttcgagag ttaactcttt aaccaaaagc gcattagcaa
     8821 aatcttccat aaaagtgctc gccacctcgg ctatacccat aggatagtcg gcatttaatg
     8881 cattttgtgc acttgaagat ataaaatgat gaatagcatg gcccatctca tgagccaagg
     8941 tcgaaacatc tctaaattcg ttgttatagt ttagcagtat ataggcagga taatcatttg
     9001 ttatagagac acagaaagca cctcccgact tccccttctt tggaaagaag tctatattac
     9061 cgccacacag catggactcg aaatattccc caaatatcgg gtctaaggac atgaaacagt
     9121 catgaactac ctcgactgct gtagcaacat taatttcatg ttttaatttt ccatattcaa
     9181 tgtttctttg attgtaagtg aggacttttt tacccaataa ttttgccttt aaatcataaa
     9241 attgggcgga aacaccaaaa ttcgccgaca ctgcttcaat tagaacgtca attgtggcgg
     9301 cgtccatatc ttcactcaac aagcgacgct catcggctcg cttaaagttt cgtagcttat
     9361 ttaaagtttt tagatagtat aaaatggcat ttatctctcg ttccgctgtt ttaatatgct
     9421 tctctaaaac ttgctccaaa gatttagatg catacttctg tactttatca tccgaacttt
     9481 tcaagtattt tgaaatttcc gaaatagaaa ttgtgatttt ctcgtgtctt tcgtttagaa
     9541 cttttactgt ttccaaagaa tagaaggtac tcaccatgtc tacccagtca ccataggcac
     9601 cctttgaaat caaattcaat agttgctcct ctttttccga caagtcgtgc tttgcagaag
     9661 caaaaagctg ttctaagtaa tgtttgtaat cttgtaggtc atgatgagac agaaactctt
     9721 tttgcttttc ttcaggaatt ttacctaatg tgagtgcgaa aaattcaagt tcattatatt
     9781 tttctgtcaa aaactcgttt aaagaattta acttcgcctt aattttgtta tcagctgaat
     9841 cgagactgga acgcaagtaa aagtaaaaac cggccctccc ccctacagaa attaaccttg
     9901 aaagcttctc atactcgtct agtgcgtttt ttaaggccgg agcagaagtt aaatagtctt
     9961 ttgactctct gtgctttgaa acaaactgct ctactgattt agcgatttcc gttctctcaa
    10021 gatcctgcgc cgcatcttca tcagatttca taagcaccga taaatcccat tgagaaggaa
    10081 actcccaaac agcagggcta tttgaaaaag ttacttttat tttcggttct ttggggtctg
    10141 cttttttggg actatctttc atagctttca cctaatttct ttccacaata taacaaatta
    10201 tggcacaata taatagatga gcccactcgc ccaagaacca atttcaatag ctgaagataa
    10261 taatttaata attaacaata ccgacgaagt tcgggagagc ttaatagatc aacttattta
    10321 ctctgcagtg ttcggctcgg gcaagaaaaa gggcgacgct aggtggataa taaaaaaaat
    10381 tggccaaaaa ctaggagtaa actttgcttc aataaacgtt ttttacaaga aacgtgctaa
    10441 agagagctcg ataaaaccat tcacagttcc ggcatttaat attaggggtc ttacatacga
    10501 cacggctcgc acacttttta aaaccgcaaa aaaattaaat acggatgctt ttgttataga
    10561 aatagcaaga agcgaaatga actactctaa acaaaacccc gaagaatttg cgacatgcgt
    10621 tatagcagca gcaattaaag aaaactacac atggccaata tatctacaag gagaccactt
    10681 tcaggcaaaa gctaaatcgc ccggcattgc agatgtaggt gaaatggaag ccttaaaaaa
    10741 attaatttct gaggcagttg ctgaaggatt ctacaacata gacatcgaca cctccaccct
    10801 agtagatctt ggacaaaaaa caatacagaa acagcaactg ccgaacatac tagccacagc
    10861 cgaactcacc gactttatta gaacaaccta cacctcacca aattacccaa caatttcaat
    10921 aggcggggaa attggccata taggaggtaa aaacagcacc gcagaagaat ttgaaatatt
    10981 tatgcaagga gtagaatctg aaactcaagg agctgcgggc ttaagcaaga taagcataca
    11041 aacaggaacc agtcatgggg gaatggtaga cgacaatgga aatatcttag atgtggaggt
    11101 agacttcgca ttacttaaaa atatatcctt gatagcgaaa aataaattta atatggccgg
    11161 agccgtgcag cacggtgcct caacactacc cgacaactta ttcaggatgt ttcctaagaa
    11221 tcttactgca gaaatacacc tagcaacagg aacacaaaat ataatatttg accatcccct
    11281 atttccggag acgctaaaaa aaggcatgga ttcttgggtt ctacaaaatc ttcagaaaga
    11341 aagaatgccc ggatggaaca acgaacaaac tatttataaa ctacgcaaaa aaacttgggg
    11401 ccaatttaaa aaagatctat gggaaataga cgcaaacaca aaaaaagcca tctttgctga
    11461 ccttcaaaaa aggttcattt ttttatttgc tgagcttgga atttcagacg ttaaaacaca
    11521 aagccaaaac taacaaacat cctatactaa atagtatttt tggtttatta tctaaatatg
    11581 acaaacacaa cgaagcaaaa aacagagccc gaacaaatca gtaaaacact taccgaagtg
    11641 gtgcaagacg aaaaaagcct tgcaataata gcgcacatag gagggatatt ctttgggatt
    11701 atagctcccc ttattatatg gattcttcag aaagataaac caaacgcgtc attctcaacc
    11761 catcatgcca aagaagcact aaatttccaa atatcagtac tactagcaag tgcggtagcg
    11821 ggacttacaa ccatagtatt aatcggattc ttgttcctac cagctgttat ggtagcgaac
    11881 gtaatatttt gtatattagc tgccatagaa gctaataagg gcctacctta caactacccc
    11941 ttctctttga ggttagtgaa gtaggatctg gtaagtttaa ggatttgaaa ggtacttaaa
    12001 atatggaaga aaatgaagca gtaattcaaa acaaagattt gccctccgaa gcagaagatc
    12061 agaaaaactc tcgcataaat aaacttaagt ttagaattaa tgggaaaaag ttactaattc
    12121 ttgggggtgc tgttgtttta attcttcttc taatctctgc cgttttagtt agtaatagta
    12181 aatctaaaaa agtcacgcca agcactgcaa acgaatcaca agagactatg aaacaaaagc
    12241 caataaaaag ttcagaatac aatccttcaa cagaagggga agtagttttg ccttggacac
    12301 caaacaatat aaatacttct catgatacat attatttatt cggtgaggtt aaaacagacg
    12361 gtgacctatt tcacgttgat ttattcacaa atggggagct tgttgatgat tatacgattg
    12421 gggtaaataa ttacctgcta gggttgaatt tcggcctaag caacagtggt ttcgtcatcg
    12481 agaagggtag agctcacaaa aatttttatt ttcctattag agatttaaag gttggcgaaa
    12541 acaatgtatc aattaaactt tacacagacg atactaaaga aaaagtatta agtgaaaaat
    12601 cggttattat cacaagatcg gatacaacct acaattcaga agtgacttta tacaacagcc
    12661 cgagagaagt tagtgctgaa gagattttca ataaagcggg catagatttt gagaacgaga
    12721 agttatggga gtactttcat aatcaaccgt cttggttctt agacaacaaa agtaacgatt
    12781 tcttctacgg caaggctggg gattggatcg actacaaaat agaggctatg ggagaaatag
    12841 gcattaaaaa tgaaaaaaca tctttgtaca gattccttta cactcatcca ggcgaaggtc
    12901 cgggtcctag cgcatacaat aactcgatat acttttacat tgttaacaac caagcatatt
    12961 tcgtaacaaa cacacttgta tcagacgagt attttgctcc aatttacctt ttttatatgc
    13021 aaccttcacc ttatatattt gaggatacgg tggctgaact ggaagaaatt tctatcaatg
    13081 agaataatac cctaattcca tctgctaccg gtgttggagt acttagatat tgtgacccag
    13141 ctaagattga gggggaaaag atttacacct atcaatcatt ttctgtatat gaaaactttg
    13201 atgtatacga ccaatacggg aattgtaaaa actttagatt ggttcctagc tactttagag
    13261 acgatacata cggcacatac cccggtgcca gtgattggaa tgacggagta accccagatt
    13321 ttaataaaat gactgttaat cttgatgatg gtaaaagtct agacagtttt tataaccctt
    13381 attggtattg gtgtgctcgt tcccgtgggt acagaaccat agatagcagt aattacttgg
    13441 aaaaagtagg taaaacttca acaggagaag atgtttatat atacagtact aatatgccgt
    13501 tcccaaagga cagccatcta ctgccctctt ccacaaaaga atatccatac tacacagcct
    13561 ctgaggaaaa ttggcaagaa tttctaaata atcaaaaaga aacttacccg gttttagtaa
    13621 tcaaaaatgt ttttggtgaa tgggtggtgt acataaatgc cgattatctt tttgctccgg
    13681 gttgctagtt gagggtttga agagtaagaa aaagggcatg gcgggataga agattgattg
    13741 gctgatatct ccaatccaca gaataccacg aagcaagctt actaatagcc cctgcgcctg
    13801 cagagaggcc cattaaataa atctcaggtt tgtcgtagcc gcaaatttct tttgaatgta
    13861 ctattgcata gttaagcata tttcttaaaa atgtatccaa attacccaca catgagctgc
    13921 cttttattag aactgctgct aagacttgtt cgacatatca agaccctcga aatttagaca
    13981 cttttagcca ctggtttcaa ggtaattagg gaatttgctt ctgttgaatc ttctttggtg
    14041 accccacggg gaataagcaa agcttgcatt tgtcttcgac aaaagtcgaa cctagcttcg
    14101 ctatagctct ctcataccag cagcatcaca tgaacaaaaa cctccaccta ccggtggaga
    14161 actttgttca tggtgacccc acggggaata agcaaagctt gcatttgtct tcgacaaaag
    14221 tcgaa
//
LOCUS       DAOSWN010000009        14162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_2716_length_14162_cov_4.460693, whole genome shotgun sequence.
ACCESSION   DAOSWN010000009 DAOSWN010000000
VERSION     DAOSWN010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14162
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2716_length_14162_cov_4.460693"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..253
                     /locus_tag="PLF29_01005"
     CDS             65..253
                     /locus_tag="PLF29_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52550.1"
                     /translation="MPKRYKPSGTDLVGKDKHAIDKMLSFNAPAYNEKETISIYNTLI
                     NSNVTNSNSRKNNNVEVK"
     gene            720..1952
                     /locus_tag="PLF29_01010"
     CDS             720..1952
                     /locus_tag="PLF29_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52551.1"
                     /translation="MPILTYDTGLYHAQSVKYITEFKLILGLANIHCRLGFNSNLFVL
                     QSALVIIDYKYIINSYLILLLSLNTVFHANKRNKSLFLANIASICFIFLFFFFNINGL
                     SNDVFATILGVILIDLLLTNEKDYSFNNQLPILITSYLITIKLSAVVFGILIIYLLVK
                     NRNKLIYGMLLSALIIAPWLVRNIMISGYLIFPYPQVDLFNLQYKVPKETAYETFYDI
                     KNWARTGTTMSMPFIEWVKTWSSNFQYIDIVSLLSLSLLFLVIFHMIIYNRKNHDFLI
                     YCCIGVVGIFFWFFTAPDTRFGMVYFVYSTYLSTYYIFKNIRKSSDVKIVYTLTLVSY
                     FLISLISDVGIWNQYRNENINYYPEVYTESLSNGISINVLEKGDQCWYTPLPCTYKVN
                     TGLKLIGTTLEDGFKIDK"
     gene            2189..2950
                     /locus_tag="PLF29_01015"
     CDS             2189..2950
                     /locus_tag="PLF29_01015"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HXK52552.1"
                     /translation="MKKLVIVADDFGTLPSINSGISFLYKNIPTLEISMVTKAKYSKE
                     AATMVKEQSIQNIGIHMELLGLEDLGRPVTAKDYIKLFKEKTYEEIEKIASEELKAFE
                     TTIGKPPTHITSHKGIHGNFKLLNFIIAYAKEKNIPIRKPVTALNVELGNQNYAAEIS
                     LSRAKLKSTNYLFAHMLGNNLDKIKNAFITDLEKVNEGETAEIVIHPAFFDERLLNYS
                     SLNYLRTRDIDLFTDQNFIDQIKKMGFKFCTYKQI"
     gene            2947..3771
                     /locus_tag="PLF29_01020"
     CDS             2947..3771
                     /locus_tag="PLF29_01020"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HXK52553.1"
                     /translation="MNTKITKKRVVFTLHDFGKSFSVNDGIVFAMNHAENIATQYSLL
                     PNGIASNQAAQIARERTDLSFDLCFTFTDFKPIGKGYKTLTDSDGYFLKANTAQWDFS
                     VLDNYNSYEIEKEIEEQYEWFLTNVGRKPTALTTQKSEHGDPKILIPITDLAKKENIP
                     LRTPAWQWFSNYAAQSYVEDLGLTFSNSVFVATKDWKGEHGYSLEEDVDLLISDIKNT
                     EGISEIVVLAGFVDQELFDMSSVSWQRGEYLNLLVRKPEILKRYATEFDIISYSKI"
     gene            4002..5645
                     /locus_tag="PLF29_01025"
     CDS             4002..5645
                     /locus_tag="PLF29_01025"
                     /inference="COORDINATES: protein motif:HMM:NF013959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HXK52554.1"
                     /translation="MQKNAKVLSIIISVTLIFNFFLQPMAAFEQNTSLADSDASFAAN
                     VLGDNVGNALSSAGDVNGDGYDDFLLGVRYGGDIEAGAVYLILGKAEGWAMGADIDDA
                     DASFEGEEAYDNAGYSIAGAGDVNNDGYDDFLIGAPDADSGLGKTYLILGKEEGWAME
                     TSLADADASFVGEYESDYSGSSVSGAGDVNNDGYDDFLIGVSDADSGIGKTYLILGKE
                     EGWAMETSLADADASFVGEYESDYSGFSVSGAGDVNNDGYDDFLIGAPDADSETGEIY
                     LILGKEGSWAMETSLADADASFEGEEAYDSAGYSIAGAGDVNDDGYDDFLLGSPWYNE
                     TEDAYEGKSYLILGKEGGWAMETSLADADASFIGEYIYDYSGQAVSGAGDVNGDGADD
                     ILIGAPGDGEDENLYGRVYLLLGTPSEEDPSEDIITNDNNNPTPKNTFKKDAKCRWQD
                     PNELTWVLLEPMDKNGVSGINLTWVQYGADKINIKIDDGTGSYPWNITEISNDGQEFL
                     PNVFAWQNIKVQAVNHCNKGKWSQEFSYTSYPEGWYNVK"
     gene            5731..6927
                     /locus_tag="PLF29_01030"
     CDS             5731..6927
                     /locus_tag="PLF29_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXK52555.1"
                     /translation="MNISLENQIKNYIDLLDIPAERLQKSGYSKENLAQELLKKLEEI
                     KSELLEVDKKLSKKINVAIIFAMWGEQNRLMPKSSSNPSGENALREKYNQLSGLFENT
                     KNFSWTLYPVDDGCPHDSFGVAQKIVAQNKLEDHVKVIALQEGVDKSFKGLSSVDESR
                     KGGSIAYGASRAIEDGANIIIYTDTDLSVHLGQAGLLLTALLTNPSLVAAIGDRNLPG
                     SIKIKDGVRWGKGVKFFRHIQRMVGKEIFSIGITDTQAAFKAFTAKAVKELIAKQKQF
                     DFAFDTDWLLNTIQTFGQSSIKQVPVVFIDSDRESTSIQQEADYPGMKTWEQLLKGIT
                     QTVSSNPNTLDKDLVETIMGLDALKLAKLVTIVPPQLENVRNEDLGKIETMSPKDIVR
                     FIKEHE"
     gene            6920..8443
                     /locus_tag="PLF29_01035"
     CDS             6920..8443
                     /locus_tag="PLF29_01035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXK52556.1"
                     /translation="MNKYLKTAAGLYSRPVSKISRIKIINILIIISLLFFGGFLRFSN
                     LGYSEFQDDEKKAVVRKINGETTAEFLLNQRKGPLQFAVTSATLLINQNKSELVLRAP
                     FTFLNLLSIVVVYFLLKEITGSKTAGFFGAFIFLTNGFIVGFSRIAQYQNLNIFFSLL
                     SLLFYRNLVTKQTKLALYSVLGTICFSLSVLAHWDAVFFIVPIVYFYIVFLIRKDITK
                     RSKLIITSINFLAGSFVILPFLLPYLNNLSANSGNLEYFDKRMGLLAYPLSRHKYIFE
                     LYNPFYALYFIGALSAIAIFKIKQSWPYMMWLIINFAGIFIFMEKPGTHIYNYFIPAI
                     FLTSFGISALAKNKHIFILFLLPLALSVSFLYLQSYKIFVDHKQEYPWDSKTIITLFG
                     TKYDTKIYDDKEVLTFGFPHFRNWKEVNEIIKSDKDGCSYITNEGKEISQIYVEEKYG
                     ILNSRRCYYIVQVVKPFITRGNGAKFAKISGKSPVYTYKKDGETLLKMYRIDNKNFK"
     gene            complement(8479..10293)
                     /gene="typA"
                     /locus_tag="PLF29_01040"
     CDS             complement(8479..10293)
                     /gene="typA"
                     /locus_tag="PLF29_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HXK52557.1"
                     /translation="MNLRNIAIIAHVDHGKTTLVDGLLKQSKTFQEHESYMTQDLIMD
                     TNEQERERGITILAKNTSVKYKGVKINIIDTPGHADFAGEVERTLNMADGALLLVDAQ
                     EGPMPQTKFVLKKALGLGLKTIVVVNKIDKRDADIDHTVGRVYDLFLDLATDESQLEF
                     PIIYAIGREAKAWTKIPDDPSLPADLSPVFDAILSFIPEPNLDPTGPFQIQVTTLAWD
                     THKGKYALGRINRGKVVAGQKVLLLKHDGSQEDCVVDKVYMTEGLKRVDIEEGKAGDI
                     VAISGIKNADIGDTLADPSSPERLPDLDLEEPTLSVSIGANTSPFKGREGDFVNSRQI
                     LARIEKEIQTNIAMKFNIDSSGQYVVSGRGELHISVFLETLRREGYELEVGQPRVITK
                     VVDGVVMEPIEEVTIDVANDYDGAVKSELGRRRGIQVFQEDLGSNTARMIFELPTKSI
                     IGLVGVLSTQTKGTAVVNTIFLRYQKMSAGMDKLRKGVLISSDTGSTTVYGLRVAQDN
                     GITFVGPGTDVYTSMIVGLNSRDKDLEINVCKGKALSNVRSLGEEAITIAPPQNMSLE
                     QSLSFLEEDELLEITPKSLRLRKKILDTTQRVRAARRK"
     gene            complement(10402..11373)
                     /locus_tag="PLF29_01045"
     CDS             complement(10402..11373)
                     /locus_tag="PLF29_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52558.1"
                     /translation="MLNGIWFPEVYRLEGSEAHHRVYHDELHTLQTIERLSTVTDLCK
                     ESLTKEQIFGLLMGMAYHDSIVFLHGRGPNFSLSNETESIAAMLYSANAINENSRDEI
                     LDYEAIVLVAVGTIAATNLRIHTEDLVGGSWPAALTRTKIIDAQDLVDAVCASGTHIP
                     VLPQVVACAVYADLGGVFSNKIEERISSSLRHFWETRPDFITFANSTNYSAKDTVEFL
                     RAVQGMSLGERGFLEMVAGPRLWAALSPVFSEEVINNLLPMRTRFNEVILDLEARLPL
                     QKALGLLKSLENVYPEEQPQMLLNEEQKKELGSILYALSAPTLQQDS"
     gene            complement(11625..12905)
                     /locus_tag="PLF29_01050"
     CDS             complement(11625..12905)
                     /locus_tag="PLF29_01050"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HXK52559.1"
                     /translation="MHKCLFKYKVLWDERDSQHFFEYIQKVQFFLSHLLWGLAGFFLA
                     GCLFLVGFKAIENRAGILAFFYSNFQELSVSYFSADFQSLPYAELIYVSDNMPSEVSR
                     GWFAAQSFLDFYRDQVQTLSNDSFVIIGEDFNNDNTIEPSLETVPKPFSPEPLTQFHE
                     GLNGGVNNKELFEELVEEIQQAQGVFGLYIKDLSSGSEYKYNADESFFGASLYKVPVA
                     VSVLKQIEDRTISLEDEVSYLRIDFYGGTGSINTYPYGTRFTVKELLDLLLKQSDNVA
                     QNMLVRYIGLSKIEEGFSNISGNPGSIFFEENITTPVEVGSIFEHLFLQIIGSNKNYY
                     LGNDGSSTIIKTMFNTYFDDRISSGLNSGLSFSHKIGSWGNTGSWHDCGIVYDSSQIE
                     ARAVVCLMSKNTTYAEFVSVAEKVGNYLSNIIAR"
     gene            12969..14156
                     /locus_tag="PLF29_01055"
     CDS             12969..14156
                     /locus_tag="PLF29_01055"
                     /inference="COORDINATES: protein motif:HMM:NF019522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HXK52560.1"
                     /translation="MSQHKKIYIEKNSHLKAFAAVLILGVLMFLGTNAQTTHAQSEFY
                     PPQLTQTAVKGMVTEVVKEQLIQNEYAKYTYQELKIKLTQEPYLNQEIIVKINETPTS
                     NSTRYEVGDKLNLLRVEDENSKISYYITDFDRTNAIVLIFFAFIICVLSVSRFWGLAA
                     VIGMVYSFAIIFKLMLPQILNGHDPLLIAILSAFLIAPASFYLSHGINRKTTIALIST
                     TIALIITGILSIIAINMSKLTGFGSEEAYFLQLAKHGEINIKGLLLAGIIIGTLGILD
                     DVTVSQASIVTQLKHANKRLSQIELYKYAMRVGHDHITSTVNTLVLVYTGAAMPLLLL
                     FINSEKSFTEVMSLEIIADEIIRTLVGSIGLILAVPITTFLAVKFADENDTSAERHCH
                     QVV"
BASE COUNT         4636 a         2737 c         2581 g         4208 t
ORIGIN      
        1 gattgagtta cttcttgata atagcaacat aaaaaccagg tgatttctct tgcagcattt
       61 ccgcatgcct aaaagataca agcctagtgg tacagatttg gttggaaaag ataaacatgc
      121 catagataaa atgttgagct ttaacgcccc tgcttacaat gagaaagaaa caatttccat
      181 atataacact ttaatcaatt caaatgttac aaactcgaat agtcgcaaga acaataatgt
      241 agaggtaaaa taatacgtag aaactaatta aatatacctt tttacagtat cctatttctt
      301 gaaaaattat tcataaatcg ccttttataa agtagtatct agtgtatatg atatttgcta
      361 tcagtgtagt gattacttgg gtatattatt tttttctttt tcagtcatgg ggaattcttc
      421 tattacaaat actcaataga aaccacacgt ctaggaaaga ataccccgtg cttcgtataa
      481 ccaccacaaa cattctaggc atgatatttc tgacagcttt aatttcagta ttaaatattt
      541 tttttaaaat tgattttccc attagactgc tggtatcttt gaacatagct attttttata
      601 aatcgctgta tagaactgcg ctgattataa tttccgaaat taaatattta ttaaaaaata
      661 gaccagtttt attttgtttc tttacaatcc ctgtttttct cctcttaact tccttgtcaa
      721 tgcctatact cacttacgat acaggtttat atcatgccca aagtgtcaaa tatattactg
      781 aatttaaatt aattctagga ttagcgaaca tacattgcag gcttgggttc aattcgaatc
      841 tatttgtatt acaaagtgcc ttagtaatta tagattataa atacattatc aattcctatt
      901 tgatacttct actttcatta aatacagtgt ttcatgcaaa taaaaggaat aagtcgctct
      961 ttctagccaa tatagcttcg atttgtttta tatttttatt cttttttttc aacataaatg
     1021 gtttatctaa tgatgttttc gcaactattt taggcgtcat tttaatagat cttttactta
     1081 ctaatgaaaa agattattca tttaataacc aattacccat actgattact tcctatctaa
     1141 taactataaa gttaagcgca gtagttttcg gaattttaat aatttatctg ctggttaaaa
     1201 ataggaataa acttatatat ggcatgttac tttccgcttt aataattgca ccttggctag
     1261 ttagaaacat aatgataagc ggctacctta ttttcccata tcctcaagtt gatttattta
     1321 atttgcaata taaagttcca aaagagacag cttatgaaac tttttacgat attaaaaatt
     1381 gggccagaac aggtaccact atgtcaatgc catttattga atgggtaaag acatggtctt
     1441 caaattttca atatatagat atagtaagtc ttttaagttt atcgctgctt tttttagtta
     1501 tttttcatat gattatttac aatagaaaaa accacgattt tttgatctac tgttgtattg
     1561 gagttgttgg tatatttttt tggttcttta cagcacccga tactagattt ggaatggtat
     1621 attttgttta ctcaacttac ttatctacct actatatttt caaaaatata cgtaaaagta
     1681 gcgatgtcaa aatagtgtat acattaactc tagttagtta ttttttaatt tcattaatat
     1741 cagatgtggg catatggaat cagtatagaa atgagaacat aaattactat cctgaggtat
     1801 atacggaaag tttgagtaat ggcatctcca taaatgtatt ggaaaaagga gatcagtgct
     1861 ggtacacacc cctaccatgc acttacaaag taaatactgg tttaaaatta attggtacca
     1921 cactagaaga tggatttaaa attgataaat aatcccattg aaatatattt aatattttca
     1981 gatgtaataa ggaaactttc agaatatagt atgggatttt tttaaacact cctacaaaca
     2041 ggtactacaa ctaaacctta tagctagagg attgatagga ttttaagcag gcgttgtagt
     2101 ataaacaatt gttatttgcg aattagtatt ataagtcaag agataataac aaaaaaatat
     2161 agataaaaag gtagtatata atacttatat gaaaaagctt gtaatagttg cggatgattt
     2221 tggaacccta ccctcaataa atagcgggat aagtttctta tataaaaata taccgacact
     2281 tgaaatttca atggttacta aagcgaaata ctcaaaagag gcggcaacca tggtgaaaga
     2341 gcaaagtatt caaaacattg gtatacatat ggaattgtta ggcttagaag atctaggtag
     2401 accagttact gcaaaagatt acatcaaact atttaaagaa aaaacttatg aggagattga
     2461 aaaaattgct tcagaggaac taaaggcttt tgaaacaact attggcaagc caccaacaca
     2521 tattacatca cacaaaggaa ttcatggaaa cttcaagctc ttgaatttta ttattgcata
     2581 tgcaaaagaa aaaaatatcc caattagaaa accagttact gctttaaatg ttgaattggg
     2641 aaaccaaaat tatgcggcag agatttcttt atccagggct aaattaaaat caacaaatta
     2701 tctttttgcc catatgttgg gaaataacct agacaaaatt aaaaacgcat tcataacaga
     2761 ccttgaaaaa gtaaatgagg gggagacagc tgaaatagta atacaccctg cattttttga
     2821 tgaaagatta ttaaattatt catcattaaa ttatttacgc acaagagata tagatttatt
     2881 tacagatcaa aattttatag accaaataaa aaaaatgggg tttaaatttt gtacttacaa
     2941 acaaatatga acacaaaaat aacaaaaaaa agagtggtat ttacgctaca tgattttgga
     3001 aagtctttct ctgtaaacga tgggattgtg tttgcaatga atcatgctga aaacatagca
     3061 acacagtatt cattattacc taacggcatt gcaagcaacc aagccgcgca aatcgcaaga
     3121 gaaagaaccg atctgtcttt tgacttgtgt tttactttta ccgatttcaa accaataggt
     3181 aaagggtaca aaacactaac agactcagat gggtacttct taaaagcaaa caccgcacaa
     3241 tgggactttt ctgttttaga caattataat tcttacgaga tagagaaaga gattgaagaa
     3301 caatatgagt ggtttctaac taatgtagga agaaaaccta cggcgctaac aacccagaaa
     3361 tcagaacatg gggaccctaa gattttaata ccaataacag accttgcaaa aaaagaaaat
     3421 attccgttaa gaactccggc gtggcaatgg tttagtaatt atgcggcgca gtcgtatgtg
     3481 gaagaccttg gcttgacatt ctctaacagt gtttttgtgg caaccaaaga ctggaagggc
     3541 gaacatggat atagcttgga agaagatgtt gacttactga tatctgatat aaaaaataca
     3601 gaaggcattt cagaaatagt tgtactggct ggttttgtgg atcaggaact attcgacatg
     3661 agttctgtaa gttggcaaag aggagaatac cttaatctac tagtaagaaa gcctgaaatc
     3721 ttaaaaagat acgccactga gtttgatatt ataagttatt cgaaaatata gttccagatt
     3781 ttttaactgt cagaaatcct gctggtattt tctgggccat atagtagtaa tttatctact
     3841 tgtattcata agagtcgtaa gttaagtcaa aaagcaataa cttctccata gctatacctc
     3901 tggggcacca tactaagtca gataaacatc gggcctctgc cctaaattgt agtgaaggag
     3961 agcattgaca acgcaaaatt gtacaactat tatttgatat aatgcaaaaa aatgcaaaag
     4021 ttctttccat tataatttct gtcactttaa tttttaattt tttccttcag ccaatggcgg
     4081 ctttcgaaca aaataccagc ttggccgact ccgacgcatc ttttgcagca aacgttcttg
     4141 gagataatgt tgggaatgcc ctatcaagcg caggagatgt aaacggggac ggttatgacg
     4201 actttctatt aggcgtacgt tacggcgggg atatagaggc cggcgcagtt tatctgatac
     4261 taggaaaggc agagggctgg gctatgggag cagatataga cgatgcagac gcttcttttg
     4321 aaggcgaaga agcttacgac aacgctggat actcaatcgc cggcgcagga gatgtaaaca
     4381 acgatggtta tgacgatttc ttaatcggtg ccccagatgc tgattccgga ctaggtaaaa
     4441 cgtatctgat tcttggtaaa gaagagggtt gggctatgga aacaagccta gctgacgcag
     4501 acgcttcttt tgtgggagag tacgaatctg attactcggg ctcctcagtc tccggagcag
     4561 gcgatgtaaa caacgatggt tatgacgatt tcttaatcgg tgtctcagat gctgattccg
     4621 gaataggtaa aacgtatctg attcttggta aagaagaggg ttgggctatg gaaacaagcc
     4681 tagctgacgc agacgcttct tttgtgggag agtacgaatc tgattactcg ggcttctcag
     4741 tatccggagc aggtgatgta aacaacgatg gttatgacga tttcttaatc ggtgccccag
     4801 atgctgactc agaaacagga gaaatatatc tgattcttgg taaagaaggg agttgggcta
     4861 tggaaacaag cctagctgac gcagacgctt cttttgaagg cgaagaagct tacgacagcg
     4921 ctggatactc aatcgccggc gcaggagatg taaacgatga tggctatgat gattttctat
     4981 taggatctcc gtggtataac gaaacagagg acgcatacga aggaaaatcc tatttaatac
     5041 tgggcaaaga agggggttgg gctatggaaa caagcctggc tgacgcagac gcttctttta
     5101 taggagaata catatacgat tattcagggc aagcagtctc gggtgcagga gatgtgaacg
     5161 gggatggcgc agatgacata cttatagggg ctcccggaga tggtgaagat gaaaacctat
     5221 acggaagggt gtatctacta ctaggcactc cttcggaaga ggatccctcc gaggacataa
     5281 taactaacga taacaacaac cccaccccaa aaaacacatt caaaaaagat gccaaatgcc
     5341 gatggcaaga tcctaacgaa ctcacgtggg ttttgctaga accaatggac aaaaacggag
     5401 tgtcaggaat caatcttaca tgggtacagt atggtgccga taaaattaat attaaaatag
     5461 acgacggcac aggcagctac ccctggaata taacagaaat ttcaaacgat gggcaagagt
     5521 ttcttccaaa cgtatttgca tggcaaaata taaaagtgca ggcggtgaac cactgtaaca
     5581 aaggtaagtg gagccaagaa ttttcttaca cttcctatcc tgagggctgg tataacgtta
     5641 agtagaaaca taaaagttac acttaaaagt tcgagaaata ggcacggaca aacaatactc
     5701 cccaacaacc ttatagtgta aaataaaaaa atgaatatat ctttggagaa tcagattaaa
     5761 aactatattg acctgctaga cattcctgct gaaagattac aaaaaagcgg ctattcgaaa
     5821 gagaaccttg cgcaagaatt acttaaaaaa ttggaagaga ttaagagcga acttttagaa
     5881 gtagataaaa aacttagcaa gaaaataaat gtagccataa tatttgcgat gtggggagag
     5941 caaaaccgac tgatgccaaa atcttccagc aatccttcag gcgaaaatgc gcttcgagaa
     6001 aaatataatc aacttagcgg tctttttgaa aatacaaaaa acttttcatg gacactttat
     6061 cctgtagacg atgggtgccc tcatgacagc tttggtgttg cacagaagat tgtcgctcag
     6121 aataagctag aagaccatgt aaaagttatt gctctgcaag aaggggtaga taaatctttt
     6181 aaaggcctgt cctcggtaga cgaatcaaga aaaggcggct caatagctta tggagcctcg
     6241 agggcgattg aggatggcgc aaatataata atctatacag acaccgacct gtcagttcac
     6301 ttaggacaag ccggcctatt attaactgct ctcctgacca acccatctct tgtagcggca
     6361 attggagaca gaaatcttcc gggctcaatt aaaataaagg acggagtaag gtggggtaag
     6421 ggcgtaaagt tttttagaca catacaaaga atggtgggta aagaaatatt ttcaataggc
     6481 atcacagaca cccaagcggc ttttaaagct tttactgcta aggcggttaa agagctaata
     6541 gctaaacaaa aacaatttga cttcgccttt gacacagact ggctgcttaa tacaattcaa
     6601 actttcggcc aaagttctat taagcaggtg ccggttgttt ttatcgactc cgaccgcgaa
     6661 tcgacatcaa tacagcagga agcagactac ccgggcatga aaacatggga acagttacta
     6721 aaaggcataa ctcaaaccgt aagttccaac cctaacacac tggataaaga tttagttgag
     6781 acaattatgg ggctggatgc cttgaaactc gcaaaactag ttacaatagt gccccctcag
     6841 ttagaaaatg tacgaaacga ggatcttgga aaaatagaaa caatgtctcc aaaggatata
     6901 gtaagattta taaaggagca tgaataaata tttaaaaaca gcggcaggct tatactcaag
     6961 acctgtatca aaaataagca gaataaaaat tattaatata cttattataa tttcgctgct
     7021 cttttttgga ggttttttaa gattttcaaa cttagggtat tcagaatttc aagatgatga
     7081 gaagaaagca gttgtaagaa aaataaacgg agaaacaaca gctgaatttt tacttaacca
     7141 aagaaagggg cctctgcaat ttgctgttac ctcagctaca ctccttatta atcaaaataa
     7201 aagcgagctg gttttaagag ctcccttcac ttttctaaac cttctgtcca tagtcgttgt
     7261 ttattttcta ttaaaagaaa tcaccggctc caaaaccgca ggattttttg gagcgttcat
     7321 ctttttgaca aacggtttta tagtgggttt ttccagaata gcgcaatacc aaaacttaaa
     7381 tatattcttc tctttgttat cattactttt ttatagaaat cttgtcacaa aacaaacaaa
     7441 acttgcactt tattcggttc ttggaacaat ttgcttttcc ttatctgtct tagcacactg
     7501 ggatgcagtt ttttttattg taccaatcgt ctacttttac attgtatttt taataaggaa
     7561 agatataacg aaacgaagta aattgattat taccagtata aattttctag ctggaagctt
     7621 tgttatacta ccttttcttc taccctattt aaataatctg tcagcaaact caggaaactt
     7681 agaatacttt gacaaaagaa tgggactact cgcctaccct ttaagcaggc ataaatatat
     7741 ttttgaacta tataatccgt tttatgcgct atattttatt ggggcattaa gtgctatagc
     7801 aatttttaaa attaaacaga gctggcctta tatgatgtgg ctgatcataa attttgcggg
     7861 gatttttata ttcatggaaa aacccggaac acatatttat aactacttta tacccgcgat
     7921 ttttcttaca agttttggaa taagcgcgct tgctaaaaat aaacacattt ttattctttt
     7981 ccttctgccc ctagcactgt cggtatcatt tttatactta caatcatata aaatatttgt
     8041 tgatcataaa caagagtatc cttgggatag taaaacaata atcactcttt ttggtactaa
     8101 atacgacacc aaaatttatg acgataaaga agtactaact tttggattcc ctcatttcag
     8161 aaattggaaa gaggtgaacg aaataattaa gtctgacaag gatgggtgtt catatattac
     8221 aaacgaggga aaagaaattt cacaaatata tgttgaagag aaatacggta ttctaaactc
     8281 gaggagatgc tactatatag ttcaagttgt taaacctttt attacaaggg gtaacggggc
     8341 taagtttgct aaaatctcgg gaaagtctcc ggtgtatacc tataaaaaag acggcgaaac
     8401 gcttttaaaa atgtacagga tcgataataa aaactttaaa taaccagcca ccgccaactt
     8461 tacgcaggta agccgctatt atttccttcg tgcggccctg acacgctgag tggtatctaa
     8521 tattttcttt cttaaacgca ggctttttgg tgtaatctca agaagttcat cttcttcaag
     8581 aaaacttaag ctttgttcta aactcatgtt ttgaggagga gctattgtga tagcctcctc
     8641 gcccaaagac ctgacattac ttaaagcttt acctttacaa acattaattt ccaagtcttt
     8701 atctctactg ttaaggccta ctatcatgct tgtgtacaca tctgtaccgg gacccacaaa
     8761 agttattcca ttatcttgag ctactcttag gccgtagaca gtggttgacc ccgtatcgga
     8821 agatattaat acgcccttac gcaacttatc catacctgcc gacatttttt ggtatcttag
     8881 aaaaatagtg tttacaacag ctgtgccttt tgtctgcgta cttaaaactc ctactaaacc
     8941 aataatgctt ttggtcggaa gttcaaatat cattcgggcg gtatttgagc ctaaatcttc
     9001 ttgaaaaacc tgtattccgc gcctccggcc aagttcgctt ttaacagcac cgtcataatc
     9061 attagcaaca tcaattgtaa cttcttctat aggttccata actaccccat ccactacctt
     9121 tgttataacc ctaggctgcc caacctcaag ctcgtaccct tcgcgcctca aagtctctaa
     9181 aaacacggag atgtgcaatt cccctcgtcc cgaaacaaca tactgcccgg aagaatcgat
     9241 attaaatttc atggcaatgt ttgtttgtat ctctttctca atgcgagcta aaatttgcct
     9301 actattgaca aaatccccct ctcttccttt aaagggagac gtattagctc ctatggatac
     9361 acttaaagtt ggttcctcta agtcaaggtc cggcaatctt tctggggagg acggatcagc
     9421 aagagtatct cctatatctg catttttaat accggatatt gccactatat cccccgcctt
     9481 gccttcttca atatcaactc tttttaatcc ttctgtcata taaaccttat ctacaacaca
     9541 atcttcttga gaaccgtcat gtttcagcaa caacactttc tgacccgcaa caaccttgcc
     9601 tctattaatt cttcccaaag catatttccc tttatgggta tcccacgcca gtgttgtcac
     9661 ctgaatttga aaaggccctg tcggatctaa attcggctcc gggataaaag ataaaatcgc
     9721 atcaaaaacc ggagacagat ctgctggtaa cgaagggtcg tcgggtattt tggtccatgc
     9781 tttggcctca cgaccaattg catatattat agggaattcc agttgactct cgtcggtagc
     9841 tagatcaagg aataaatcat aaaccctccc cactgtatga tctatatcag cgtcgcgttt
     9901 gtctatttta ttaacaacta ctatagtttt caatcccaac cctaaggctt ttttcaaaac
     9961 aaattttgtt tgtggcatag ggccttcttg agcatctacc agaagcaggg caccgtctgc
    10021 catatttaaa gttctctcaa cttctcccgc aaagtccgca tggccgggag tatctataat
    10081 atttattttt acacctttat attttacgga agtgttttta gccaatattg ttataccacg
    10141 ctctcgctcc tgctcattgg tatccataat caaatcctga gtcatgtaag actcgtgttc
    10201 ttgaaaagtc ttggattgct ttaataaacc gtcaactagt gtggtttttc cgtggtccac
    10261 gtgtgcaatt atggctatat ttcttaaatt catgcggagt gattatacat aaataaagag
    10321 aaaatacata acaactggac caaaaaactt ttaacatggt cgggttttgc ccccggcctt
    10381 gaagcaatag aatagtctgt gctaagagtc ttgttgcaga gtaggggcac ttaaagcata
    10441 taatatgctt ccaagttcct tcttctgctc ttcatttaat aacatctgtg gttgttcttc
    10501 agggtagacg ttttcaagac ttttaagaag tcctagtgcc ttctgcaagg gcagtcttgc
    10561 ctccagatct aagattactt cgttaaaacg agtccgcatt ggaagcaaat tgttgattac
    10621 ttcctctgaa aacacaggag agagagcagc ccaaagtcta ggacctgcta ccatctctag
    10681 aaacccacgc tcccccaaag acatgccttg tactgctctt aaaaactcga cggtgtcttt
    10741 tgctgagtag ttcgtcgaat tagcaaaagt aataaaatcg ggacgtgttt cccaaaaatg
    10801 tcggagtgac gaggagattc tttcttcgat tttattagaa aaaactcctc ctagatcagc
    10861 gtaaactgcg caggcaacta cctgaggcaa aactggtatg tgcgtcccag aagcacacac
    10921 cgcgtcaact agatcttggg catcaattat ttttgtacga gtaagagcgg ctggccaaga
    10981 tcctccaaca agatcctcgg tatgtattct caagttagtt gctgcgatag tccccacagc
    11041 tacaagcaca atagcttcat agtctaggat ttcatcccgt gaattttcgt ttattgcgtt
    11101 ggccgaatat aacatagccg ctatggactc ggtttcgttg ctgaggctaa aattaggacc
    11161 tcggccatgt agaaaaacga tcgaatcgtg gtatgccatg cccattaaaa gtccgaagat
    11221 ttgttcctta gtgagggatt ctttacataa atccgtaact gtagacaacc tctctatagt
    11281 ttgaagagtg tgaagctcgt cgtgataaac cctatgatga gcctctgatc cctctaacct
    11341 atatacttct ggaaaccaaa ttccatttaa cactaagtgt tgtagggaat tttgagcgtg
    11401 cccactagta gcacaagcta taacattact agaaataaac ccttctactg ttttggccgc
    11461 cggtacactc agccccaggc ctacaaatct actaaaaagc tcttcgctag aaaaggctac
    11521 atcaagcctt caccttttgt ctccagatgc agcccttgca atgcctccat cttcaggttt
    11581 tgggcctcct ttggtcgcca tataactttt cctctcatta aattttatct tgcaataatg
    11641 ttactcaaat aattaccaac tttttcggcc acactaacaa actctgcgta tgttgtattt
    11701 ttactcatta agcaaactac agccctagcc tcaatttgag agctatcata cactataccg
    11761 cagtcgtgcc aagatccggt atttccccaa cttcctattt tatgcgaaaa agaaagtccg
    11821 gaatttaggc cggagcttat tctatcatca aaataagtat tgaacatggt tttaattatt
    11881 gtagaagatc catcgtttcc taaataatag ttcttgttag agccaatgat ctgaagaaaa
    11941 agatgttcaa atatcgagcc cacctcgaca ggggtagtga tattctcttc aaaaaaaatg
    12001 cttccgggat taccggaaat gttcgagaac ccctcttcga ttttagaaag gccaatgtat
    12061 ctgacaagca tgttttgcgc aacattgtct gattgtttta gtaataaatc cagcaactcc
    12121 ttgacggtaa atcttgtccc gtaagggtaa gtattaatac ttcctgttcc tccgtaaaaa
    12181 tctatacgca aataagaaac ttcgtcttct agcgaaattg tccggtcttc aatttgcttt
    12241 agtaccgaca cggcaacggg aactttgtac aaagaagctc caaaaaaact ttcgtcagca
    12301 ttgtacttat actcactgcc cgaggacaaa tctttaatat ataaaccaaa aacaccttgt
    12361 gcttgttgaa tctcctcaac aagttcttca aataactctt tgttgttaac accgccattt
    12421 aaaccttcat gaaactgcgt aagtggctcc ggcgagaaag gcttaggcac tgtctctaaa
    12481 gaaggttcta ttgtattatc gttattgaaa tcctctccta ttattacaaa agaatcgttc
    12541 gaaagtgttt gtacctgatc acgataaaaa tctaaaaaac tttgtgccgc aaaccaacca
    12601 cgactaactt ctgagggcat gttgtctgaa acatatatca gttctgcata gggcagcgat
    12661 tgaaaatcgg cagaaaaata tgagactgaa agttcttgga aattactata aaaaaatgca
    12721 agaatcccgg cgcgattttc aatcgcctta aatcctacta aaaacaggca gccggcaaga
    12781 aaaaagcctg caaggcccca caataaatgt gaaaggaaaa actgtacttt ttgaatatac
    12841 tcaaaaaaat gctggctatc tctctcgtcc cacaaaactt tatacttaaa aagacactta
    12901 tgcataatgt aattttatca ccctggcgtg tttttagtct caaacattaa atgtataatt
    12961 aaatttaaat gagccaacac aaaaaaattt atatagaaaa aaactcacat ttaaaagctt
    13021 ttgcggctgt actaatttta ggcgttctta tgttcctcgg aaccaacgcg caaacaaccc
    13081 acgcacaaag cgagttctac cctccgcaat taactcaaac agcagtaaag ggaatggtta
    13141 ctgaggtagt taaagagcag cttatccaga acgaatacgc aaaatacaca tatcaagaac
    13201 taaaaataaa actgacgcag gaaccatacc taaatcaaga aattatagta aaaataaacg
    13261 aaactcctac atctaacagc acaagatacg aagtaggaga taaactcaat ctgcttaggg
    13321 tggaagacga aaactcaaag atctcgtact acataaccga cttcgatcgt acaaacgcta
    13381 ttgtactaat ttttttcgct ttcattatct gcgttttatc ggtcagtaga ttttgggggc
    13441 ttgccgcagt aataggaatg gtctattcct ttgcgattat ttttaaactc atgttaccgc
    13501 aaattctaaa cgggcacgat cccctgctca tagctatact tagcgccttt ctaattgcgc
    13561 ccgcctcttt ttatttatca cacggtataa atagaaaaac cactattgcc ctaatctcaa
    13621 caacaattgc cctaataata accgggatcc tctccataat tgcaattaac atgtctaaac
    13681 tcacaggatt tggctccgaa gaagcatatt ttttgcagct tgcgaaacac ggcgaaataa
    13741 atataaaagg acttttattg gccggcataa taattggaac gctgggaata cttgacgacg
    13801 taactgtatc gcaagcctca atagtaactc agcttaaaca cgcaaacaaa aggctatccc
    13861 aaatagagct atataaatac gccatgcgtg ttgggcacga ccatataacc tcaacagtta
    13921 acactcttgt actcgtttac accggggccg caatgccact acttctactt tttattaatt
    13981 cagaaaagag ctttaccgag gttatgagtc tagaaattat tgcagacgaa ataattcgaa
    14041 cgcttgtagg aagcatcggg cttattcttg ccgtacccat aacaacattc ctcgccgtca
    14101 agtttgccga cgaaaacgac acatctgcag aacgacactg tcaccaagtg gtataattgc
    14161 ta
//
LOCUS       DAOSWN010000010        14084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_2733_length_14084_cov_4.580726, whole genome shotgun sequence.
ACCESSION   DAOSWN010000010 DAOSWN010000000
VERSION     DAOSWN010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14084
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2733_length_14084_cov_4.580726"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..803)
                     /locus_tag="PLF29_01060"
     CDS             complement(120..803)
                     /locus_tag="PLF29_01060"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52561.1"
                     /translation="MEKGSHILQSSNYNASKTYLDNLLQDIYHTDPENNPDVLIISPK
                     NNRNSIGIDQIRIANTYCTLRPHHYSYKTLIVRSAHKMTVQAQNAFLKTLEDMGVSML
                     IILETPAPEKLLQTVRSRCKLITLTNIDSELNNKQPKQPNTASGRHDLKQYMTIQQRL
                     EWAEELSKEDADVILDVLTIIGNQLKERMEQKDLLCLEALLEITDAIQNTNVNKRFAL
                     EYFALTINS"
     gene            941..2710
                     /locus_tag="PLF29_01065"
     CDS             941..2710
                     /locus_tag="PLF29_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HXK52562.1"
                     /translation="MSNTITNKGIEDVLFERGLINDDQLSAIKFEHVNTGRSTSQIIK
                     DRAYVSTQDFLKAFGEVYGIEFVDLSELKINPNILEIIPASLLKKYQCVPFEQNGDVL
                     SVAMVDPLDLQNIEFMERKSGFKVKPYITTPADIDKVLADTQGKAISEEISAALEELS
                     QNTLKLKGDSGDLNADEATISDAPIARIVGMILETAVKIGASDVHIEPRETDTRLRYR
                     VDGVLQEKRTFPREMHDSIIARIKILANMKIDEKRIPLDGRFKVAVGNTGTDLRVSTL
                     PTVYGEKVVIRLLKEEGAIFTFKDLGLRGLSLRRLEEALIKPVGMVLVTGPTGSGKTV
                     TLASALAKLNSVRVNIVSIEDPVEIRVPGVSHVQVNPQAGLTFATGLRAFLRQDPNII
                     MVGEIRDGETAELAIHAALTGHLVLSTLHTNSSSGALPRLLDMGVESFLLASTVNAIL
                     AQRLVRKICENCKEEYEAPAEVEEDIRKILDDIKSSDIIMHKDPDIAKAIQSVKSDKI
                     KLYKGKGCDKCGQSGYKGRIGIFEVLENSTNIAALTLEHKPESIIQEAAVAEGMLTLM
                     KDGYLRVVEGITTLEEVMRVSKG"
     gene            2731..3804
                     /locus_tag="PLF29_01070"
     CDS             2731..3804
                     /locus_tag="PLF29_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014901944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HXK52563.1"
                     /translation="MAINASQLLEKVIEVNASDLHLVVGMPPSIRVNTVLSRLDDYGI
                     LSIEDIQYFISKILSQDQQNLFDLNKELDLSVALSNKARFRVNVFYQKGYPSVALRLI
                     PMLVPSLDSLNLPPIIKNIGELKQGLVLVVGPTGNGKSTTIASMIDYINETRSEHIVT
                     VEDPIEYIFTSKKSLIEQREMYLDTHSWEAALKSVLRQDPNVVFIGEMRDKETMESVL
                     NIAETGHLVFATLHTNSAAQSLDRVISSFPETQQLQVRLQLSQVIEAVISQRLLPSPN
                     KGTVPAVELLLATDAVRSLIREGKAYQIDNVISTGATYGMMTLDASLALLVSQNYVDL
                     EEAMKRSLNPENLRRMLKMGGSK"
     gene            3805..5007
                     /locus_tag="PLF29_01075"
     CDS             3805..5007
                     /locus_tag="PLF29_01075"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXK52564.1"
                     /translation="MALYEYTGVDNLGTRRSGKVDARSVDTAIALLKNQNIHVLNIAE
                     SKGTLISNIGDLRGVPFSEVVSFTRQMSTMIGAGLPIAKTLDVLSQQTTNTRFRVILG
                     DILRDVEGGTSIAASFSKYPDVFSKTYQSLVRAGEASGKLDQILLRLADTMESQRELR
                     AKFKTAMIYPVLVMVAMVGVFFMLMVVVVPKLADMYESLDAELPIITQLMINLSDFMV
                     NNLIVIILGMFGGLYLIRSFSKTPGGREFFIKLTYVLPVFGAVNKKRDLTEFCRTLGL
                     LLTSAVPIVEALEIVHDVVSYYHYKNGAKSAAQTIEKGGTLSGYLKSDSSFPPIIGNM
                     AATGEETGKLDEVLDRLAEFFNRETQHAVEGLSAALEPLILIMLGGMVAVLIISIITP
                     IYKITTSI"
     gene            5079..5558
                     /locus_tag="PLF29_01080"
     CDS             5079..5558
                     /locus_tag="PLF29_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXK52565.1"
                     /translation="MTKLNKRNEKGFTLVELLIVIAIIGVLAGAILIVINPAQLLAKS
                     RDSQRLQDLDSLNKAITLAIAEQEITITTTTGNSGTGTRSVTGGGWVGFTLFTGKTAG
                     LGKYIPTLPVDPTNTGNYVYSYAADATSGSYELNAVLESTDNQAKMGTDGGSSATKW"
     gene            5882..6793
                     /locus_tag="PLF29_01085"
     CDS             5882..6793
                     /locus_tag="PLF29_01085"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HXK52566.1"
                     /translation="MDYITVLLYAMLFISGAFLGSFINVVADRASKGQSFLFGRSKCE
                     TCNKVLSPRDLIPVLSYLLAKGKCRHCKEKLSLWYPISEIVTGALFAGLAYYLNLFVN
                     VSYAYVWVFYVYFAIVGMSYIALFLADIKYMLLPTKIVVPTIAFVLLFLAGNVGFTII
                     SSYKALQADDFGKYLIESGYWQTQILLMLRSVAYSVGSAAAIALFFWILTKIKQGQAM
                     GKGDIKLAFLIGLVNGFPFNVLAIFLGFLMGALYSVGLLALKKKTLQSTIPFGPFLIV
                     GSVVSIVYGAQLLNWFFSLSNLVPAAM"
     gene            complement(6939..7337)
                     /locus_tag="PLF29_01090"
     CDS             complement(6939..7337)
                     /locus_tag="PLF29_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52567.1"
                     /translation="MEGFDRSAAARFPLYIILSEPVTPLLINLGPSQIPVSVSMIPGL
                     EKILLFGSCPVFWSAELFDNTASREQNKELFLEQTMELNNKNPNIKNVPARSIKRIFV
                     VFFADENVAKRFFTVDSMGRIYKCYTKDRW"
     gene            7336..7536
                     /locus_tag="PLF29_01095"
     CDS             7336..7536
                     /locus_tag="PLF29_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52568.1"
                     /translation="MTLTKNNVSTEIIIDTNTLNLYYAPHSDSANHENYPISSLEDLF
                     LKLKDGDFVSYFPGKFIIVLYY"
     gene            7556..11305
                     /locus_tag="PLF29_01100"
     CDS             7556..11305
                     /locus_tag="PLF29_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52569.1"
                     /translation="MYLIKNIFLRLKIKSFITVANPLAAGALFLATLIFSASSFSIFA
                     QACAPDSGEHHTYDEYFVNPRCGLWDFPCIGSQCVVNEGYGNQCNAGIANNCLAKTNQ
                     LSEGECTAIGYAFPCYYTNIWPQPPCSTCGSTAAAANPLCGSDEYTPSVCTVAPDAAC
                     PTSTVPIPDTDTPVTIEVRGVKNVPRDEGHVYGLMWFNSENSSNAVRVDLERQNDALG
                     NYTGIYRKTFTPCATYGKCYSGTANEMLWFDVWMSNDNIGSDTFYCDDANFWTTPPTA
                     SEDPPYASLAIENLTRSLYYSTADAVFINDNERVRVIGQGFSNPSSPDINQMNLRSCS
                     SSQTCLDANSSTTVATSSTGIVETELSFSAGAVIRYLVNARNTTAAGANQWCVGYTDP
                     GSFVKCVRNNLPNPSYKDLITLRINNSPHASLSATDTDTGTNYTNSSFTISQGTTLSF
                     TANASDTYAGGNPGIVTGIYLKKCNDANNCSGLTQVSEVASCTSDSGVSTCSTQPSQY
                     ITFNTPGTYKFLVNAADNADPKAWCYSYPNYNYSALDAYKPCKRGSDTLPNVLTITVT
                     AVNQAPTGSIETINCSLGGRVCDTDGDRNIIVVPGNIISFQAVGQDDGGINYTRIAYC
                     ETANCASPTTLTSASGAVAFYNRTFFPADIGKSYYFFVTADDNGSPTLSCSGNPSAST
                     YCDRPSNTIDKYTVTVSAAPNALPEGTIQLGTSAPGTIQTLNLGDSGTIVANVTDSDG
                     YINSSQIIYCPSGFNNCSPADGSGLILSTCGVNVSQCSIGYTFNTVGPAYFVVRATDD
                     DSGVCSGQSAAAASRCDRSLPLDEDRLTVNVVNNTGTTESLTGITYNVSDVAACSNIT
                     TSPRLTSGAVQESLSVTYINHAGTSYNGNWTDANGNFEISNIPIPENGDLCATGSFTD
                     SVDSRYRYDLVCYSDGTTVQTLNTSSTCASNITLPGSGSNNYFGYVKVDTYQEAWYRV
                     IGGIVVSLGDIANTITNPYDSALAFLATRLSLTNVNVSAFSRGAFDVTNGKIAQPDAN
                     EASSQRYFSNMPPELTFWDTSFNFDPPAGTLDSPLPSGNIWGLTKDVYKISAASLNGI
                     LSSSTDVLTYNVDSKNLAIIYVIADSPTDVLVKRRLNTGTGKLMFIGSENVNFVFDSS
                     VGVGCNTNTVNSIFRAAIITQGSVYFNTSGTNNDSCIVVDGPLIAGVPNDNTTGRIYL
                     FRNMSAAPTGNRIPTEQVRYNPQYIYLTDETTGIMVTDVIWEELE"
     gene            11302..12249
                     /locus_tag="PLF29_01105"
     CDS             11302..12249
                     /locus_tag="PLF29_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52570.1"
                     /translation="MMLSTISKMNYRRINEIKSEAGQVLLFVVVTMTVALALGVGVSL
                     QTISTISDVADTDTAQRVLAAAEGGGERALSLKQTDLANLANAKKANNLPGYQNLCAP
                     AFGDSVTGEFLCKLNFTPAADSGDPILLESQIGVEYFTFNKETQDGTPYYEFALGQNK
                     VKEVVMNESKSIADIKVCWTGEDGLFYTSYSNSANFYRQDIICPVVGCPSGVGSNAIS
                     STASDISGFNQCATLSTGDYGTTPKGLRFRTLGADTRVAVVTASPFFPPQGIKITSTG
                     LLAVPGQPPIKKIVRVYRSFAYAPAPFDFAIYSESELSK"
     gene            12474..12908
                     /locus_tag="PLF29_01110"
     CDS             12474..12908
                     /locus_tag="PLF29_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012519428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXK52571.1"
                     /translation="MKNIKNNSKGFTLIELLIVIVIIGILAGIVLTVLNPARQQQRAR
                     ETVLRANTEKVCLALHSCAVTSSSALNCDDWTDIGAINPTGTPTGSTYPISPSPATAA
                     GDTITITGTLGSCVFTCNYNFTNSTAGQLALGTGATCLIGVQ"
     gene            13031..13570
                     /locus_tag="PLF29_01115"
     CDS             13031..13570
                     /locus_tag="PLF29_01115"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HXK52572.1"
                     /translation="MYKIKKISQSKNKGFTLIELLIVVSVLGILGGVLTTVINPKVQR
                     AKAQDSVYATNIQKLAEGIEAYYITGNGVAYPAEDPSGSLSNVIADPNNNLSKYIATW
                     PTDAAYEYRVSSDSTNACLSVPMASDPDVRIKYVFKSSTSGACSGRVVRDCTYSCYEG
                     SSFNTNLDGCNSLTGNACN"
     gene            13591..>14084
                     /locus_tag="PLF29_01120"
     CDS             13591..>14084
                     /locus_tag="PLF29_01120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXK52573.1"
                     /translation="MYNKRNKNLNPGSGFTLIEILIVVVIITVISGAILSLINSEGLR
                     GKSRDAQRVADIKKTQSALELYFADNRQYPDTSSDKSELADISSFLSSYMSVTPTDPL
                     NTGQVDNLCGNSAVYKYVYQSDGSEYYLGVNMEDSRSAEPSPCTDFSLFCNSAEATCY
                     AVRNP"
BASE COUNT         4133 a         2660 c         2979 g         4312 t
ORIGIN      
        1 ataatttcgt tacttacaac tattttcgca agctagcaga tgtagttcgc acaacatgct
       61 gggctttgca gtgcaaagaa attacagtaa gccacctgcg taagctggtg gtagttgatc
      121 tatgaattta ttgttaaagc gaagtattcc agagcaaagc gcttgtttac atttgtgttt
      181 tgtatcgcat ctgttatttc aagtagagcc tcaagacaaa gcaaatcttt ctgttccatt
      241 ctctctttaa gttggttccc gattatagtc agaacatcaa gtattacatc cgcgtcttct
      301 tttgaaagtt cctctgccca ctcgagcctt tgctgaattg tcatatattg ttttaaatca
      361 tgtcttccgg aagcagtgtt tggttgtttc ggctgtttgt tatttagttc agagtctatg
      421 tttgttaatg ttattaattt gcatcttgag cgaactgttt gaagcaattt ttcaggagcg
      481 ggagtttcaa gtataataag cattgagact cccatgtcct ctagtgtttt cagaaaggcg
      541 ttttgtgctt gaaccgtcat tttgtgggcg ctacgaacaa taagagtctt atagctgtaa
      601 tgatgtggtc taagagtaca atatgtgttc gcaattctaa tttgatctat gcctatggag
      661 tttctattgt ttttaggcga tattattaaa acatcggggt tgttttcggg atcggtgtgg
      721 tagatgtctt gtagtaaatt atcaagatat gttttggaag cattataatt tgaactttgt
      781 aaaatgtggc tacctttttc catagataaa ttaaagtata tatcttttct tattgcatac
      841 gttaccaaat tgctgttatg attaaaagtg atttatacaa aatgttatat tatacaagtt
      901 aatcttgata atctaacggt ttgtaaaata tcgctgccct atgtcgaata caataaccaa
      961 caaaggaata gaagacgtat tgtttgaaag aggccttatt aatgacgatc aactttcggc
     1021 aataaagttt gaacacgtta acacaggaag aagtacctcc caaataataa aagatagggc
     1081 ctatgtaagt actcaggact ttctaaaagc ctttggagaa gtctacggca tagaatttgt
     1141 agatttatcc gaacttaaga taaaccctaa tattctagaa attattcctg caagtttact
     1201 aaagaaatat cagtgtgtgc cttttgagca aaatggagat gtgttgtctg tggctatggt
     1261 tgatccgctc gatttacaga acattgaatt tatggaaaga aaatccggat ttaaagttaa
     1321 accgtacatt actacccctg ctgatataga taaagttctt gccgacacac agggaaaagc
     1381 aattagtgaa gaaattagtg ctgcgcttga ggagttaagt cagaatactt taaagcttaa
     1441 gggcgattcc ggagatttga atgccgatga agcaaccatt agcgatgcac ctatagcaag
     1501 aatcgtaggc atgatacttg aaacagctgt aaagattggc gcctcagatg ttcatatcga
     1561 gcctagagag acggacacac gtttgcgtta tagagttgac ggtgttttac aggaaaaacg
     1621 aactttccca agagaaatgc acgactctat tattgctcgt attaaaatcc ttgccaacat
     1681 gaaaatagac gaaaagagaa ttcctttgga cggtaggttt aaggttgctg tcggtaacac
     1741 agggactgat ctaagagttt caactctccc aactgtatat ggcgaaaagg tagttatccg
     1801 attattaaaa gaggaaggtg cgatttttac atttaaagat ttaggtttgc gcggactgtc
     1861 tttacgaagg ctcgaggaag ctttgattaa gcctgtgggt atggtgttgg taacaggtcc
     1921 tactggttca ggtaaaactg ttacattggc ttcagctctt gcgaagctaa attcagtgcg
     1981 tgtaaatatt gtgtcgattg aagatccggt tgaaattaga gttcccggag tatctcatgt
     2041 gcaagttaat cctcaggctg gtttgacttt tgccaccggg ttaagagctt ttttgcgcca
     2101 agaccctaac ataataatgg taggagaaat tagagatggt gaaactgccg aactggcaat
     2161 acatgccgct ttaacaggcc accttgtttt atccacgtta cacacaaact cctcatccgg
     2221 agctttacca aggcttcttg atatgggagt agaaagcttt ttgcttgctt ctactgtcaa
     2281 cgcaattttg gcccaaagat tggttaggaa gatttgtgaa aactgtaaag aagagtatga
     2341 ggctcctgcg gaggtcgaag aagatatcag aaagatacta gatgacataa aaagttcaga
     2401 tattattatg cataaagatc cagatatagc taaagcgatt cagagtgtaa agtcagataa
     2461 gattaagttg tacaaaggta agggctgtga taagtgcggg cagtctggtt acaaaggacg
     2521 aataggtatt tttgaggttt tggaaaattc taccaacatt gcggctctaa ctttggagca
     2581 taagcccgaa tcaataattc aagaagctgc tgtggcagag ggcatgctca ctttaatgaa
     2641 agacgggtat ttaagagtag ttgagggcat cacgaccctt gaagaagtaa tgagggtttc
     2701 gaaaggatag cacatcggtg cttaaataaa atggcaataa acgcttcaca gttattggaa
     2761 aaagtaatag aggttaacgc ttcggatttg catttggttg tcggaatgcc cccttccata
     2821 agagttaata cagttctatc tcgccttgat gattacggca tattaagtat tgaagatatt
     2881 caatatttta tttcaaaaat actttcgcaa gatcagcaaa atctttttga tttgaataaa
     2941 gagctagatc tttcagtggc tttaagcaac aaagctagat tcagagttaa tgttttttat
     3001 caaaaaggat acccttctgt agctttaaga cttattccca tgctcgttcc gagcctagat
     3061 tccctaaatt taccgcccat tataaaaaat ataggcgaat taaagcaggg gttagtgtta
     3121 gttgtcggtc ctacaggtaa cggcaaatct actacaatcg cttctatgat agattacatc
     3181 aatgaaactc gttctgagca cattgttacc gttgaagatc ctattgaata cattttcacc
     3241 agcaaaaaat ctttaataga gcaaagagaa atgtatttag atactcattc ttgggaagcg
     3301 gcgctaaaat ctgttttaag acaagacccg aacgttgttt ttattggaga gatgcgggat
     3361 aaagaaacaa tggagtctgt tttgaatatt gctgagactg gtcatttagt ttttgcaact
     3421 ttacatacaa actcagctgc gcaaagcttg gatagagtta ttagctcgtt tcccgaaact
     3481 cagcaactgc aggtaaggct ccaactatct caggtcatcg aagcggtaat ttctcaaagg
     3541 ctactcccct cccctaataa gggcacagtt ccggccgttg agttgttgct tgccactgat
     3601 gcagttagaa gtcttattag agagggcaag gcttatcaaa ttgataatgt tataagcacc
     3661 ggtgcgacgt atggaatgat gactttagat gcaagcttgg ccttgctggt ttcacaaaac
     3721 tatgtagatc ttgaagaggc catgaaaagg tcgctaaatc ctgaaaattt gagaagaatg
     3781 ttaaagatgg gaggttctaa gtaaatggct ctttacgaat atacgggtgt agacaacctt
     3841 gggacaagaa gatccgggaa agtagacgca agatctgttg atacggctat tgctctgtta
     3901 aaaaaccaaa atattcatgt tttaaatatt gccgagagca aaggaactct aattagcaat
     3961 atcggagatc tacgaggagt tccgttttca gaggtagttt cttttacaag gcagatgtcc
     4021 acaatgatcg gggccggtct tccaatcgca aaaactttgg atgtgctatc gcagcaaact
     4081 acaaacacca ggtttagagt tattttagga gatattttac gtgatgtgga gggaggaact
     4141 tctatagcag cttcgtttag taagtatccg gatgtctttt ccaaaacata tcaatcttta
     4201 gtgcgtgcgg gagaagcttc gggaaagttg gatcaaatat tattgcgtct ggccgatact
     4261 atggaatctc agcgagaatt aagagcgaaa tttaaaaccg caatgatata tccggttttg
     4321 gtaatggttg ccatggttgg tgtctttttt atgctaatgg tagttgttgt tccgaaacta
     4381 gccgatatgt acgaaagcct agatgcggaa ttgccaatta ttactcaact tatgattaat
     4441 ctttcggatt tcatggtcaa caatttaata gtcataattc ttggaatgtt cggcggatta
     4501 tatctaatta gatctttttc aaagactccc ggaggaagag aattttttat caaactaacc
     4561 tatgtcctgc ccgtttttgg cgcagttaac aaaaagaggg atctaacaga attttgtcga
     4621 accctcggtt tgcttcttac ttctgctgtt cctattgttg aggctctcga gattgtacac
     4681 gacgtagtct cttattatca ctataaaaat ggggctaagt ccgcggccca gacaattgaa
     4741 aaaggaggaa ctctctcggg gtacttaaaa agcgattcca gctttccccc gataatagga
     4801 aatatggctg ctacaggtga ggaaacgggt aagctcgatg aggttttaga tagacttgct
     4861 gaatttttca acagagaaac gcagcatgct gtagaaggtc tgtctgcagc tcttgagccg
     4921 ttgattttaa taatgttggg aggtatggta gctgttctaa tcatttcgat aattactcct
     4981 atttacaaaa ttacaacctc tatctaggtt atttgaggat gtttttgtag ggcagtttag
     5041 ctttgtaatt aaatagaaaa ttgaaagggg gtgaagaaat gacaaaacta aacaaaagaa
     5101 acgaaaaagg ttttacatta gttgaacttt taatcgttat agcaattatt ggtgtacttg
     5161 caggtgcaat attaatagtt attaaccctg cacagttatt ggctaagtcc agagattcac
     5221 aaagactaca ggatttagac tctctaaata aggcaataac attagctatt gcagagcaag
     5281 agataacaat aactaccacc acaggtaact ccggaacagg aactaggtct gtaacaggag
     5341 gaggatgggt cggctttaca cttttcacag gtaagaccgc cggtttagga aagtatattc
     5401 caacacttcc tgttgatcct acaaacacag gaaactacgt gtattcatac gcggcagatg
     5461 caacttccgg atcttatgaa ttgaacgctg ttttagagtc aacagataac caggcaaaaa
     5521 tgggaactga cggaggctcc agcgcaacta agtggtaagt aggtacgaac ttggaactca
     5581 ttgatccgac cccgtagtaa ttgtgtgcgc ttcgtgatcg cgcatacttt ttatggtctt
     5641 taactgagtt aactaagtta aatattttct ttagacttat gtctatgtct gaggaaaagg
     5701 ttaagtatgc atctgaggtg cggagcccaa agggctccgc accatcaaaa aaaatactgc
     5761 aatcccctcc tctctttacg gcaagtattc tgtaaaactc ttcaccttta aagaattttt
     5821 gttatactaa tttaagttaa tatttattta aactgcctag cgtaatgaat tggagaaaat
     5881 tatggattat ataacagttt tactttatgc aatgctcttt atatcaggag cttttttggg
     5941 atcttttatt aatgtagtgg ccgatcgtgc cagcaaaggt cagagctttt tatttggccg
     6001 ctctaagtgc gagacctgta acaaggtttt gtcgccaaga gatttgattc cggtgctgtc
     6061 ctatttacta gcaaaaggaa aatgcaggca ctgtaaagag aaactatctt tgtggtatcc
     6121 gatatccgaa attgttaccg gagctctttt tgcaggtttg gcttattatc tgaatctttt
     6181 tgttaatgtt tcatacgctt atgtttgggt gttttacgta tattttgcaa tagtcggcat
     6241 gtcctacatt gccttgtttt tagcagatat caaatatatg cttttgccga caaaaatagt
     6301 tgttccaaca atagcttttg tattgctgtt tttagccggt aatgtgggct ttacaattat
     6361 atcctcatat aaagctttgc aggcagatga tttcggtaag tatttaatag agtcggggta
     6421 ctggcagaca cagattttgc tcatgctaag gtctgtagcc tattcagtag gcagcgcggc
     6481 ggcaattgct ttattctttt ggattttaac caaaattaag cagggtcaag ctatgggtaa
     6541 gggggatata aaactggctt ttcttattgg tctggtaaac ggatttcctt ttaatgttct
     6601 cgcaattttc ctaggattct taatgggcgc actttacagc gtaggtttat tggcattaaa
     6661 aaagaaaacc ttacaaagca caattccttt tggaccattt cttatcgtag gcagtgtagt
     6721 gtccatcgtt tacggagcac aattactaaa ttggttcttt tctctatcta acttagttcc
     6781 cgctgcaatg tagttttgta ggaaaattct tatttggttt gatttaattt gtgaggggcc
     6841 cgcatctaaa gatgcgggcc cccacccttt cgcccatccc cttctctacg ttactctcat
     6901 tctttgaaat tttcttttca agacaactgc tttgttgact accatctgtc ttttgtataa
     6961 catttgtata tgcgacccat actgtctact gtaaaaaatc gtttagccac attttcgtct
     7021 gcaaaaaaga ccacaaaaat tcttttaata cttctagcgg gcacattttt aatatttgga
     7081 tttttattat taagttccat ggtttgctct aaaaatagct ccttattttg ctcccggctt
     7141 gcagtattat caaataattc ggctgaccaa aaaacagggc aagaaccgaa caacagtatc
     7201 ttttcaagcc cgggaatcat tgaaacagaa acgggtattt gggatgggcc taagtttatt
     7261 agtagcgggg tgacgggctc tgaaagtatt atatataatg gaaatctggc ggcagccgat
     7321 ctatcgaaac cttccatgac gctgacaaag aataatgttt ctacagaaat aattattgat
     7381 acaaacacct tgaatcttta ttacgcacca cattcggact cggcaaatca tgaaaactac
     7441 cctatttctt ccctcgaaga tctcttctta aaattgaaag acggtgattt tgtatcatat
     7501 tttcctggaa agtttataat tgttctgtac tattaaggct gcaggtatta acaaaatgta
     7561 cttaattaaa aacatatttt tacgattaaa aataaaaagc tttattacag ttgcaaatcc
     7621 tttagcagcc ggtgctttat ttttagcaac actaattttt agtgcttcga gctttagtat
     7681 ttttgcacag gcatgcgctc ctgatagtgg ggaacatcac acctatgatg aatactttgt
     7741 caacccgagg tgtggtcttt gggattttcc ttgtataggc agtcaatgcg tcgttaatga
     7801 aggctacggt aatcaatgca acgccggtat agccaacaat tgtttggcga aaaccaatca
     7861 actgtctgag ggcgaatgta ctgccattgg ttatgctttt ccttgttact ataccaatat
     7921 ttggccacaa ccgccgtgct ccacatgtgg gagcaccgcg gctgcagcga accctctttg
     7981 tggatcagat gaatataccc catctgtgtg cactgttgcc cccgatgctg cctgccctac
     8041 tagcaccgtc ccaatacctg atacagacac gccggttacc attgaagtta gaggtgttaa
     8101 gaatgttcca cgtgacgagg gccatgtcta tgggttgatg tggtttaata gcgagaattc
     8161 tagtaatgca gtacgtgttg accttgaaag gcaaaatgat gctcttggaa attatactgg
     8221 aatttatagg aaaactttta caccttgtgc tacttacggc aagtgctatt ctgggaccgc
     8281 caatgagatg ttgtggttcg atgtctggat gtctaatgat aacataggtt cggacacatt
     8341 ttattgcgat gacgcaaact tctggaccac ccctcctaca gcgtctgagg atcctccata
     8401 cgcaagctta gctattgaga atctaacaag gtcactttat tattctacgg ctgatgccgt
     8461 tttcataaat gacaatgaaa gagttagagt tatcggacaa ggtttttcta atccaagttc
     8521 tcctgacata aatcaaatga atttaagatc gtgcagttct tctcaaactt gcctcgatgc
     8581 gaactcgtcg acaactgttg caactagttc taccggtata gtagagaccg agctctcatt
     8641 cagtgccgga gcggttatta gatatttggt taacgctagg aatactactg cggcaggagc
     8701 taatcagtgg tgcgttggat atactgatcc gggctcgttc gtaaagtgcg taagaaataa
     8761 tctacccaac ccttcctata aagatcttat aactttaaga attaataact ctccgcacgc
     8821 atcgctttca gctacagata cagatactgg aactaactac acaaattcta gttttacaat
     8881 ttctcaaggt acaacactaa gctttacggc taacgctagc gatacctatg caggcggaaa
     8941 tccgggtatt gtaaccggaa tttatctaaa aaaatgcaat gacgccaata attgctccgg
     9001 tctgacccaa gtctctgagg ttgcaagttg cactagcgac tcgggagtct caacttgttc
     9061 tacacagcct tcgcaataca taacctttaa tactcccgga acttacaaat ttttagtaaa
     9121 tgctgcagat aatgctgacc cgaaggcctg gtgctacagt tacccaaatt ataactattc
     9181 ggcccttgat gcatataagc cttgtaaaag aggctcagac actttgccga atgtgttaac
     9241 aatcaccgtg actgccgtta atcaagcacc tacaggttcc atcgaaacta taaactgttc
     9301 cttaggagga agagtctgtg atactgatgg ggatcgtaat attatcgtag tcccaggtaa
     9361 cataatttct tttcaggctg ttggtcagga tgatggtgga atcaactaca caagaattgc
     9421 ttattgcgag actgccaatt gtgcaagccc tacaacgctg actagcgctt ctggggctgt
     9481 cgcattctac aatcgcactt tctttccggc agatataggg aagagctact acttctttgt
     9541 aacggcagat gacaatgggt ctcccacact tagttgttca ggaaatcctt cagcctcaac
     9601 ttattgcgat aggccttcta acacaataga caaatacact gttactgtga gcgcagctcc
     9661 aaacgcattg cctgaaggaa ctattcaatt aggtacttcg gcacccggta cgatccagac
     9721 tctaaatctt ggagattcag gtactatcgt agctaatgta actgacagcg atggctacat
     9781 taactctagc caaatcattt actgccctag cggctttaat aattgcagcc cagctgacgg
     9841 tagtggtctg atactttcaa cttgcggcgt gaacgtttca caatgttcca ttggatacac
     9901 ttttaatact gttgggcctg cctattttgt tgtcagagct accgatgatg actccggtgt
     9961 gtgttcgggg cagtctgccg ccgcagcttc cagatgcgat aggtcccttc ctctcgacga
    10021 agataggttg acagtaaatg tcgttaacaa taccggtacc accgaaagct taacgggtat
    10081 tacctataac gttagtgatg tggcggcatg tagtaatatt acgacctcgc caaggctcac
    10141 ttcgggtgct gtacaggaga gtctttcggt cacctatatt aaccacgcag gtacttcata
    10201 taacgggaac tggacagatg ccaacggaaa ctttgaaatc agcaatatac ctattccgga
    10261 aaacggagat ttgtgtgcga ccggtagttt taccgacagt gtcgattcaa ggtacaggta
    10321 cgacttagtt tgttactcag atggaacaac cgtgcagaca cttaacacaa gctcgacctg
    10381 tgccagcaat attacactcc caggctctgg aagcaacaat tatttcggtt atgtgaaagt
    10441 agacacttac caagaagctt ggtatagagt tattggaggt atagttgtaa gtttaggtga
    10501 tatagctaat acaataacca atccttatga ttccgccctt gctttcttgg caactagact
    10561 aagccttact aacgtaaatg tctcagcttt tagccgcggt gcttttgatg tgacgaatgg
    10621 caaaatagca cagccagatg ctaatgaagc ttctagccaa agatatttca gcaacatgcc
    10681 gcctgagtta actttttggg atacatcttt taattttgat ccccctgctg gcaccctaga
    10741 ttctcccctg ccttcgggaa atatttgggg acttactaaa gacgtatata aaatttctgc
    10801 ggcctcatta aatggtattt tgtctagttc tactgatgta ctgacatata atgttgattc
    10861 taagaattta gcaataatat atgtaattgc agacagccca acagatgtgc tcgtaaagcg
    10921 aagactgaac acaggaacag ggaaactaat gtttatagga agtgagaatg ttaactttgt
    10981 ttttgacagt tcggttggag ttgggtgcaa taccaatacg gttaacagca tatttagagc
    11041 agccattatt actcagggta gcgtgtattt taatacttct ggaaccaaca acgacagctg
    11101 tatagttgta gatggacctc tcattgcggg tgtacctaat gacaatacta ccgggagaat
    11161 atatttattt agaaacatga gtgcggcccc aaccggtaat agaattccta ccgaacaggt
    11221 tagatataat ccgcagtata tatacttgac cgacgagact accggtatta tggtcacgga
    11281 cgttatttgg gaggagctcg aatgatgtta agcactatct ctaaaatgaa ttacagaaga
    11341 attaatgaaa taaaatcgga ggcaggccag gttctattgt ttgtggttgt aaccatgaca
    11401 gttgcccttg ctttgggagt aggcgtatct ctacaaacca tctccactat atctgatgtt
    11461 gcggatacag atactgcgca aagagtgttg gccgcagccg agggtggagg agagagggct
    11521 ttatctttga aacagacaga tttagcaaat ttagcaaatg caaaaaaagc aaataacctt
    11581 ccagggtacc aaaatttatg tgccccagct tttggagatt ctgttacggg ggaatttttg
    11641 tgcaagttga actttacgcc tgctgcagac tcgggtgatc ctatattact tgaatctcag
    11701 attggcgttg agtactttac atttaataag gaaacccaag atggaacacc ctattacgaa
    11761 tttgcacttg gacagaacaa agtaaaagaa gttgttatga acgaatccaa atcaatagca
    11821 gatataaagg tgtgttggac cggagaggat ggtttgtttt atacatcgta tagcaatagt
    11881 gcaaattttt atagacagga cattatttgc ccagttgtgg gatgtccttc aggagtgggt
    11941 tcgaacgcca tatcttctac agcaagcgat atttccggct ttaatcaatg tgctactcta
    12001 agtaccggtg attatggtac tactccgaag gggcttcgat ttagaacttt aggagccgac
    12061 actagggttg ccgtagtcac cgcatcgccc ttttttcctc cccagggtat taaaataacg
    12121 tctacaggtc tacttgctgt tcctggacag cccccgataa aaaaaatagt tagagtttat
    12181 aggtcttttg cttatgctcc tgctcctttt gactttgcga tttattccga atctgaatta
    12241 agtaaataaa ccttatagta tagctatttt actatttaaa aaatttaatt tctaaataag
    12301 cccacccgct tgacactccc ccgctatttg atgcatactt actgtatgag ttttctgccc
    12361 aacacctttc tctcactctt ctataatttg tttaataaaa gagtttctgt tttaacaatc
    12421 acctcacggc atacaagatt tattagattt tatcttggag ggaggtgtaa gatatgaaaa
    12481 acattaaaaa taattcaaaa ggttttacac ttattgagct cttgatagtc atcgttatta
    12541 ttggtatctt ggcaggtatt gtgctcacgg ttttaaatcc agcaagacaa caacagcgag
    12601 caagagaaac agttttgaga gctaatacag aaaaagtgtg tctcgcactt cattcgtgtg
    12661 cggtaacaag ctcctcagct ttaaactgtg atgattggac cgatataggt gctataaatc
    12721 cgaccggtac gcctacaggc tcaacttatc caataagccc tagccctgcg acagccgctg
    12781 gtgacacaat aacaattaca ggcactctag gttcctgtgt atttacttgt aattacaact
    12841 ttactaactc aacggctggt caattagccc ttggcacggg cgctacttgc ctcattggag
    12901 ttcagtagtt tttcagtgaa ttctatgccc cttctccaga aggggcattt tctttttctg
    12961 aacaccttaa aaaatgttgt atatttgtat cgttcagaaa gtgtgttaac atttatatag
    13021 taaactgcct atgtataaaa taaaaaaaat ttcacaatca aaaaataaag gctttacgtt
    13081 aatagagttg ctcattgtag tttctgtatt gggcatattg ggaggagttt taaccacagt
    13141 tataaaccct aaagtacaaa gagctaaagc tcaggattct gtttatgcaa caaatattca
    13201 aaagcttgca gagggcattg aggcatatta cattaccgga aatggcgtag cgtatccggc
    13261 tgaggaccct tcgggatctt taagtaacgt aatagcagac ccgaataata acttatcaaa
    13321 atacatcgca acatggccga cggatgctgc ttatgaatat agagtttcca gtgattccac
    13381 aaacgcttgc ttaagcgtgc ctatggcatc agatcccgat gttcgcataa agtatgtttt
    13441 taagtcttcg acctctggag cttgttctgg aagagttgtt agagattgta cgtacagttg
    13501 ttatgagggt tcaagtttca ataccaacct tgatggttgt aattctttga ccggaaatgc
    13561 ctgtaattaa tttaatgggt tttttacatt atgtataaca aaagaaacaa aaatttaaat
    13621 cccggttcag gatttacatt aatagagatt ctgatagtag tcgtgataat tacggtcatt
    13681 agcggagcaa ttttatcttt aataaactcc gaaggcttaa gaggcaaatc tcgtgatgct
    13741 cagagagttg ctgatattaa aaaaactcaa agtgctttgg aactatattt tgctgacaac
    13801 aggcagtacc ctgatacatc ttcagataaa tctgagctgg cggatataag cagttttctg
    13861 agcagctaca tgagtgtaac tcctacggac cccttaaata ctgggcaagt tgacaacctg
    13921 tgtggcaaca gcgcggtcta taaatatgtc tatcaatctg atggttcaga gtattattta
    13981 ggagttaata tggaggatag taggtcagcc gagccttccc cttgtacgga ctttagcctt
    14041 ttttgtaact ctgccgaagc aacctgttat gcggttcgta atcc
//
LOCUS       DAOSWN010000011        13617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_2862_length_13617_cov_4.148872, whole genome shotgun sequence.
ACCESSION   DAOSWN010000011 DAOSWN010000000
VERSION     DAOSWN010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13617
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2862_length_13617_cov_4.148872"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /gene="tyrS"
                     /locus_tag="PLF29_01125"
     CDS             complement(<1..854)
                     /gene="tyrS"
                     /locus_tag="PLF29_01125"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HXK52574.1"
                     /translation="MSKVSTDKEKIKDILTRGVHEIFTYKELEEKLLSGKQLHIKLGI
                     DVTGPLIHLGHAVVQRKIRDFQELGHRVTLIIGDFTTLIGDHSDKVDMRDETNTHDIA
                     ENEKTYVEQFFKTVIPEQVEIRHNSEWLKPLDFNQIINLAKQFTVSQMLDRETFMLRY
                     KANKPIGLDEFLYPLMQGYDSVVLKADVEMGGTDQTFNLLAGRKLMPAFNLKPQSAVV
                     MKLLTGSDGRPMGKSLKNFIPVTASAIDMYGMVMSIVDEVIFDYFELSTRIPLEDIEK
                     MKKEMNNGA"
     gene            956..3649
                     /locus_tag="PLF29_01130"
     CDS             956..3649
                     /locus_tag="PLF29_01130"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HXK52575.1"
                     /translation="MFKNLFFLFGAKPKYRSIQRNKKNRFKFIPFDKAFGKSGEKVKI
                     DKNNVSPKLLVATYLATGALIAVILGIVVLIIVFATFSRDLPDPNKLLERDRELSTRL
                     YDRNGKPLFEVYGEKNRQLIQVSDVSPNLVYATLATEDANFYSRRGFFLKGMLRALVN
                     MVKGEGLQSGSTITQQVAKNAALSQDRTITRKVKEIILALQLENKYSKEQILQMYLNE
                     TPYGGQNYGVLTAAKAYFNKHPRDLTLAEAAYIAGLPQSPTSYSFYGPTPEKGIARKN
                     YVLSLLKDYGWQGEDGKRHYITEEEYERAANEELLFQKPDIQFEAPHFVFYTKDYLSQ
                     LFGPDMVEQGGLQVTTTLDLDLQREAEKIVREEVDKSAYLNVGNGALVAVQPGTGNIL
                     AMVGSKDYSADSAPEGCTPGIAGEDGCVFDPNVNVTTSLRQPGSSIKPITYATMLEQG
                     YTAAYPFLDVESKFAGSAPDKPYIPKNYDGKYRGPMSMRRSLANSLNIPAVKALEIVG
                     IQNMLNTAEEMGITTLTRKNEYGLSLALGAGETKLLEMTNAFAVFANNGEYATPTPII
                     EVKNARGEVIYKAVPAKHNAISPETAFIISDILSDDGARSDVFGTGSLLNIPGHKVAV
                     KTGTTNDLRDNYAVGYTPIVAVGVWVGNNNNEAMSNVASGITGATPIYHRFMAAFLEG
                     KEEQKFEAPSNVEKVTVDSLTGMLPYADFSQRPEWFIKGTEPTSPSEWYQRIEICKPD
                     GLLASQSCREADKTSVETFIDIQAELEKWQVYVDKWIGENFSGDSKYFPPRTVSSLKF
                     DGGDVQKDTNPVVSIANFKNGSDAPLEFRLKVEVSSPNDIDYVKIYLDGNEVTKDKSE
                     PFGYNFTFTSADVGKHEFKASAVDEDGREGDVKISLEIVPY"
     gene            complement(3695..4477)
                     /locus_tag="PLF29_01135"
     CDS             complement(3695..4477)
                     /locus_tag="PLF29_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52576.1"
                     /translation="MYDTCGKALKLETNNAQKVGHSGAFTESLSPVDALEILNSAGFL
                     EFQNAEERLRATRQQGARSAHESRLIAIETASGVLEGLLQLGSMEGGVIANPADIKRR
                     VTNFYKAATMMEKEMTEEEAIFLILSLGILPEDEKDSLAKPQTRVIARNELDTLLQLL
                     AIIDGNEEIGTLLHGESDTWRQFAIADTAGKTANRILQIFGPAKPYTTDTGSDGRNGK
                     ETVGDVISEAIAKALQSPIGQEDLQVRTLGPKPIFRPAVDTN"
     gene            complement(4754..6826)
                     /locus_tag="PLF29_01140"
     CDS             complement(4754..6826)
                     /locus_tag="PLF29_01140"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HXK52577.1"
                     /translation="MKIPLEWLKQYVAINKTAFELASSFTFLGLLLDKPIEKSEFGNE
                     ILDLEHRMDRADWLGIIGCARDLAAFENVSLKMPEMHTQELAMLPEKEKVKITVKDKG
                     FVNRFYTRVIYNVKVKESPEWLKKRLIEYGMVPINNIVDITNYVMVELGQPLHAHDLS
                     KMETREITFRKAIKGESITTLLGESITLDEDITILSTNDKPTIIGGIVGGEAVAIDQS
                     TTDILLDSGSYDQTSVRKNSRKLKIINETVQRYDKFLHPYNNKLAIDRATKLILDIAG
                     GSAYENGDYFPDSERWLPKNMRLRFDRILKIGGLLIEKDKIKQILTNLDYKILKEEEE
                     ALVLEVPYFRTDVCVEDDIVSDILRIYGFSNIPMAKMTTTPPKEITPTSYVLEEKIRD
                     ILCSIGLHEHSTDPLVPADPENQRQVKLVNALTEEKAGLRYTIKETLKPILTHYAKLN
                     LRQPEIFELGKTYTLEGDPKIYENYKEIKTLQVLTTLRDGESVLDLNKRVRKILASLF
                     LNLNIDQKEYELKESPSSQNTEERSETNKPARATILVKDTEVGELYVDSFVLYTERLS
                     QALKDNLAVANKRLALHNAHTMDFDITIVCKEKRSLGPVVKNIEKIAGVGRITALDVY
                     QDGVDLKSATKAVTIRIYSTAQKAEELQRIKNLVVEEITRVEGLRTKNLTNQDQEGSW
                     DLPLIGIN"
     gene            complement(6823..7839)
                     /locus_tag="PLF29_01145"
     CDS             complement(6823..7839)
                     /locus_tag="PLF29_01145"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF013568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HXK52578.1"
                     /translation="MQQNITEIQKQFELDLETATSEAKINEVYLKYLSKSAGLTTMLV
                     KKIPSLSSEEKKLYGPKINTLIEEQKNKINDKKNLLAEESLNDPLLDLTLPEKATNAG
                     FLHPTTQVIRQLNEFFRYSGFSVAEGPEIETALFNFRKLNLPEGHPATDLQDTLFIQQ
                     LGDEMDIVLRTHTSSVEARILTNYEPPIRVVVPGKCFRNETLSSTNGAYFNQYQGFVV
                     DKGITIQHLMSTLEQAHKYLYGEDTKVRFRYKYYPEVSPGVGVDMQCKFCGGEGCDVC
                     KYRGWIEALGSGMIHYNTLKMCGIDPEIYTGFAFGMGLDRLVMQKFGVSDLRTLYGGK
                     LAYL"
     gene            8601..9575
                     /locus_tag="PLF29_01150"
     CDS             8601..9575
                     /locus_tag="PLF29_01150"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HXK52579.1"
                     /translation="MIKRVGRQGEPVERQANEALKILGITRLVEPKDPRVPSGDAVVV
                     SKRIGAFCVIDRVGGSVNGPEVKGDSRAAGIVARAVEKRIEDVSRPIDSTKLAVEFLL
                     GLQSDAKSELEAVGRYERHSAEKYIGATLTAVLVGKVKIAVLNVGDCHAVYFPRKGKT
                     YEPPKHLTLDDTRLVQILAAREGAERELLLKQQKAMAAMEDPSLYTIPGNTLPIGMDT
                     FLRTTAEVTNMVSSFASTGSVEGNVEIYDRSDLGPGLLLLMSDGTHEVCSDPALLRVL
                     NGLKPRQNPAEAVRDLVIAERNRLGQGRCRYKIDDASNICVTLGQPKL"
     gene            9815..10654
                     /locus_tag="PLF29_01155"
     CDS             9815..10654
                     /locus_tag="PLF29_01155"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HXK52580.1"
                     /translation="MPHEQTDTEHPEMGEFRIKHFNDQEILDTVCVEGVCSALKGPGT
                     AVEQFALPAEAVGLVLVHARSTHGHGPRDMEADPTTAAARQASEIFKEHLVQHLARRP
                     QYGNDYDALLASYQAIEPALLPRGDNLAISVACLLVRQEGVAFSNLGDTRVYMVNPKT
                     GTARSLTLDTPATAHRREYDKAQAFRDQDGARILVDGPRNPRSGFVGNSNFPPAIEVS
                     NRGFLGLNEAWLVVTTREINSCLTALDLAQLSGALNQPVLFQRDVLNRVQKGAVAVIA
                     LGH"
     gene            complement(10757..11110)
                     /gene="rplT"
                     /locus_tag="PLF29_01160"
     CDS             complement(10757..11110)
                     /gene="rplT"
                     /locus_tag="PLF29_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010077031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HXK52581.1"
                     /translation="MTRVKGGPRARNKHRSIIKLAKGYRGTRSKLFRRANEAVLRAGE
                     HAFAGRRIRRRDMRKLWISRISGALTQHEINYSTFMHILPKTGIVLNRKMLSEIAIND
                     PKAFEEVVNKVKAIK"
     gene            complement(11114..11437)
                     /locus_tag="PLF29_01165"
     CDS             complement(11114..11437)
                     /locus_tag="PLF29_01165"
                     /inference="COORDINATES: protein motif:HMM:NF013771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HXK52582.1"
                     /translation="MKTRKTLSKRVKITKNGIILKKQNRTGHLKRKWSANKKTRKRGL
                     GAVVSKGYIKNIKQMLGKSGSKITLNVVESENVEAAATETKSNSKATTSKKVSKKKVA
                     PIEKE"
     gene            complement(11440..11961)
                     /gene="infC"
                     /locus_tag="PLF29_01170"
     CDS             complement(11440..11961)
                     /gene="infC"
                     /locus_tag="PLF29_01170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HXK52583.1"
                     /translation="MKQEKSKYYKTNDRIRYPELRIVDDGDGENLGVLSTSEALAVAR
                     SKELDLVVITENAKPPIAKILDFNKYLYKERKKSSEIKAKAKKSKLKEFRFGPYIDEG
                     AIKVKTDRAREFLMDGNQVKVTVVMKGREQAHPEIGVEKVEKFVAALTDIAKLEDQIR
                     IQGRNIIAVLNKG"
     gene            complement(12121..>13617)
                     /gene="thrS"
                     /locus_tag="PLF29_01175"
     CDS             complement(12121..>13617)
                     /gene="thrS"
                     /locus_tag="PLF29_01175"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HXK52584.1"
                     /translation="IQEIADRGEKVSVYIFGSPESKYYDVDLCAGPHVDNTSKVKAFK
                     LLSVAGAYYKGDEKNKMLQRIYGTAFDSQEALEAHLALLEEAKNRDHRKIGKEMDLFT
                     FADDVGAGLPLWTAKGTIIREEIEKWAKETEEEWGYVRVATPHIGKHTLYETSGHLPY
                     YKDDMYSPMDIDGEDYYLKGMNCPHHHMIYKSQPRSYRDLPMRMAEYGTVYRYEQSGS
                     LFGLMRVRSIAQNDAHIYCTLEQAEEEFLISLKLHEYYYETLGLTKDDYRIVFGLPDE
                     TKREKYHGDKELWDEAEAMMKRAVEKSGIRSSDDIGGAAFYGPKTDFNVKSSIGREFA
                     ISTVQLDLYMPQRFNLEYTAADGSKKICAVIHRAPLGSHERFVGFLIEHFGGAFPVWL
                     APEQVEIIPINEKVLDYANEVHRSLRKAKLRSKVNDRDDTMQSKIREAQHQKLPYMLI
                     LGGREQEQNTVSIRLRTGEQINGVSVEEFIRIVTNVRDSKSLALWEDK"
BASE COUNT         3517 a         2827 c         2732 g         4541 t
ORIGIN      
        1 gcaccgttat tcatttcttt cttcattttc tctatgtcct ctaaaggtat ccttgtagaa
       61 agttcaaagt aatcgaatat aacctcgtcg acaattgaca tcaccattcc atacatgtca
      121 attgcggatg cagtaactgg tataaaattc tttaggcttt ttcccattgg tcggccatcg
      181 cttccggtaa gcaatttcat aactactgcg gattgaggct tcaaattaaa cgcgggcatt
      241 aattttcttc cggctaagag gttaaaagtc tggtctgtac ctcccatctc tacatctgct
      301 tttagtacga ccgaatcgta cccttgcatt aagggataca agaattcatc gagcccgatt
      361 ggtttgtttg ctttgtaacg caacataaaa gtttctctgt ctagcatttg ggaaacagta
      421 aactgctttg ctaggttgat gatctgatta aagtctaaag gttttaacca ctcagagtta
      481 tgtcttattt ctacctgttc gggaattact gttttaaaaa attgctctac atatgttttt
      541 tcattttcgg ctatgtcgtg tgtgttagtt tcatctcgca tgtctacttt atcggaatga
      601 tcgcctatta aagttgtaaa gtctcctatt atcaaagtta ctctatgccc aagttcctga
      661 aaatctctta tttttctttg tacaaccgca tgccccaaat gaataagagg gcctgtaaca
      721 tcaattccta attttatgtg aagttgtttt cctgataaga gtttttcttc caattctttg
      781 taggtgaata tttcgtgcac tcccctagtt agtatgtctt taattttctc tttgtcggta
      841 ctgacttttg acattgagta attatagcat tatgcaaaga tatttcgcat aaccaaatct
      901 gtatcaaata cctacaatta aggagctttg ttcataaatg ttataattac ctgatatgtt
      961 taagaacctg tttttcctat ttggtgccaa acctaaatat agatcaattc aaagaaataa
     1021 gaaaaatcga tttaagttta taccgttcga taaagctttt ggtaaatccg gagaaaaggt
     1081 aaagattgat aaaaataatg tatcgcccaa gcttcttgtg gctacgtatt tagcaaccgg
     1141 ggcgttaata gctgtgattt taggcatagt cgttctaatt attgtctttg caactttttc
     1201 tagagatctg cccgacccga ataagcttct tgagagagat cgtgagctat ccacgagact
     1261 ttatgataga aacggtaaac ctttgtttga ggtttatgga gaaaaaaata ggcaattaat
     1321 tcaggtttcg gatgtttctc ctaatctggt ttatgcaaca ttggctacgg aagacgccaa
     1381 cttttattca cgccgaggct tttttcttaa aggaatgtta agagcacttg ttaatatggt
     1441 taaaggtgaa ggtcttcaaa gtggctcgac aattactcag caggtagcaa aaaatgcagc
     1501 cttgtcgcag gatcgcacca taaccagaaa ggttaaagaa ataattttag ccttacagct
     1561 ggaaaataaa tactcaaagg agcaaatttt acaaatgtat ttaaacgaaa ctccatatgg
     1621 agggcaaaat tatggtgttt tgactgccgc aaaggcttat tttaataaac acccacgtga
     1681 cctgacgttg gccgaagcgg catatattgc aggtttaccc caaagcccga ctagctattc
     1741 tttttacggc ccgactccgg aaaaaggtat agcaagaaaa aattatgttc tgtccctgct
     1801 taaggattat ggttggcagg gagaggatgg taaaaggcac tatattactg aggaagagta
     1861 tgaacgtgca gcaaacgaag aacttttatt ccagaagccc gatattcaat ttgaagcacc
     1921 tcattttgtc ttttatacca aagattatct ttctcagtta tttggcccgg acatggtgga
     1981 acaaggagga ctacaagtca caacgacact cgatcttgat ctgcaaaggg aggcagaaaa
     2041 gatagtaaga gaagaagttg ataaatcagc ttatcttaat gtagggaacg gcgctttggt
     2101 agctgttcag cccggcacag ggaacatttt agctatggtt ggttctaaag actattccgc
     2161 tgattccgct cccgaaggat gtacgccagg aattgcaggt gaagatggtt gtgtttttga
     2221 ccctaatgtt aatgtcacta catccttaag gcagcccggt tcctccatta agcctattac
     2281 ctacgctacc atgctcgagc aaggttacac agccgcttat ccgtttttag acgtagaatc
     2341 caaatttgca ggctctgctc ctgacaaacc ttatattccc aaaaactatg acggaaaata
     2401 tcgagggcct atgtctatga gacgctctct tgctaattcg ctcaatatcc cagcagtaaa
     2461 agctctggag attgttggaa tacaaaatat gctcaacaca gcagaagaga tgggaataac
     2521 aacactaacc aggaaaaatg aatacggctt gtcgttggct ttaggcgcag gagaaacaaa
     2581 gctacttgaa atgacaaatg cttttgcggt gtttgctaat aatggtgaat acgcaacacc
     2641 aactccaatt attgaagtca aaaatgctcg aggtgaggtt atttacaaag ctgtgcctgc
     2701 aaaacacaac gctatttcac ctgagacagc ctttattata agcgatattc tttctgatga
     2761 cggtgctcga tctgacgttt ttggaacagg aagcttatta aacattcctg gtcataaagt
     2821 tgcagtaaag acgggtacaa ctaatgatct tcgcgataac tatgctgtgg ggtatacccc
     2881 aatagtagca gtaggagtat gggttggtaa taataacaac gaagctatga gtaatgttgc
     2941 ttcaggtata accggcgcca cccctatcta ccaccgcttt atggccgctt tcttagaagg
     3001 caaggaggag caaaagtttg aggctccatc aaatgttgag aaggtaactg tggattctct
     3061 gacaggcatg ttaccctatg ctgatttttc acagcgcccc gagtggttta ttaaaggaac
     3121 agaacccaca tccccaagtg agtggtatca gcgaattgaa atttgtaaac cggacggctt
     3181 gcttgcgagc cagtcatgca gagaggctga taaaacttct gtagaaactt ttattgatat
     3241 tcaagcagag cttgaaaaat ggcaagttta cgtagacaag tggattggag aaaacttttc
     3301 gggagactcc aaatactttc ctccacgtac agtttcttct ttaaagtttg atggcggaga
     3361 tgtgcagaaa gacactaatc cggttgtaag tatcgcaaat tttaaaaacg gatctgatgc
     3421 ccctttggag tttagattaa aagtcgaggt ttcctcccct aatgacatag attacgtaaa
     3481 aatttattta gatggaaacg aagtcacaaa ggataaatct gagccctttg gatataattt
     3541 tacatttact tccgccgacg ttggtaagca tgagtttaaa gcttccgcgg ttgacgagga
     3601 cggccgcgaa ggcgacgtta agataagttt agagatagtg ccttattaag taaatattgc
     3661 taagacaggc tagctcggat ctttttgtta gtatttagtt cgtatccacc gcaggtctaa
     3721 aaataggttt tggtcctaac gttcttactt gtaaatcttc ttggcctatt ggagattgta
     3781 acgctttcgc aatggcctct gatataacat ctccaaccgt ttctttgccg tttctgccgt
     3841 cactacctgt gtcagttgtg tatggtttag ccggtccgaa gatttgcaaa atgcggtttg
     3901 ctgtttttcc cgccgtatcc gcaatagcaa attgacgcca tgtatcactt tctccatgta
     3961 gtaaagtacc aatctcctca ttaccatcta tgatcgctaa gagctgaagc aatgtgtcga
     4021 gttcattcct tgctattacc cgagtttgag gctttgccaa ggaatccttt tcatcttctg
     4081 gtaaaattcc aagtgagagt attagaaaaa ttgcttcctc ttccgtcatt tctttttcca
     4141 tcattgttgc tgctttataa aagttagtaa ctctacgttt tatatcagca gggtttgcta
     4201 ttaccccacc ctccatcgat cctaactgta gcaaaccttc gagcactccg ctagctgtct
     4261 ctatagctat tagcctactt tcatgagcac ttctcgcccc ttgttgtcgg gttgccctta
     4321 acctttcctc cgcattttga aactcaagaa atcctgcaga attgaggatt tccagagcat
     4381 cgactgggga aagtgattcg gtgaaagcac ccgaatgtcc tactttctgg gcgttattag
     4441 tctctagttt cagcgccttt ccgcaggtat cgtacaactg aaataaagta gctttacgtt
     4501 gatttcgttt actccagtaa ttaggacttc ctatcttctc ttccccctcg atgttctcag
     4561 ttgttactgc ggccagatca tccttagtct atgtgcatct tccgcaagct gcttcacggt
     4621 ttccgtacct ggttctgttt gtaattgcaa atggggtttt ctcatcctta tttcctttaa
     4681 aattattatt tatcctgtaa ttatacattt tttttacaca ttttagcaac tccgaaaagc
     4741 caacacttgc agcttaatta atgcctatta gaggcaggtc ccagcttcct tcttggtctt
     4801 gatttgttaa gttttttgtt cttaaaccct cgactctagt gatttcttct acaaccaaat
     4861 ttttaatacg ttgcagctct tcagcttttt gagcggtgct atatattctt atggtaacag
     4921 cttttgttgc ggattttaaa tctacgccat cttggtagac gtcaagcgct gttattcggc
     4981 caactcctgc tatcttttca atatttttta ctacgggccc aagagatctt ttctctttac
     5041 aaactatggt aatatcgaaa tccatcgtat gtgcgttgtg cagagctaat ctcttattag
     5101 ctacggctaa gttatcttta agcgcttggg atagcctctc ggtgtacagg acgaaactgt
     5161 ccacatataa ttcccccacc tcggtgtctt tcactaagat cgttgctcta gcaggtttgt
     5221 tagtttcaga tctttcttct gtgttttgag agctggggct ttcttttagc tcgtattctt
     5281 tttgatctat attcaggttc aaaaaaagcg aggcaagaat ttttcttacc ctcttgttca
     5341 aatcgagcac tgattccccg tctcttaaag ttgttagaac ttgtagtgtt ttaatttctt
     5401 tgtagttctc gtaaattttg ggatccccct ctaaggtata tgtttttcct agctcaaaaa
     5461 tttcaggttg tcttaaattt agttttgcat agtgcgttaa gatcggttta agagtttctt
     5521 ttatagtata tcttagacct gctttctctt ctgttagggc gttaactagt ttaacctgcc
     5581 tttggttttc aggatcggca ggcacaagtg ggtcggtaga gtgctcgtgt agtcctatgc
     5641 tgcataatat gtctcgtatc ttttcttcga gtacataact tgtgggtgtt atttcttttg
     5701 gaggcgttgt tgtcattttt gccatcggta tgttcgagaa cccgtaaatt cttagtatgt
     5761 cagaaactat atcgtcctcg acacagacgt ctgttctaaa gtaaggaacc tcgagaacca
     5821 acgcctcctc ctcctctttt agtattttgt aatcaagatt tgttagaatt tgttttattt
     5881 tgtctttctc tatgagcaac ccgcctattt ttaggattcg atcaaacctc aaccgcatat
     5941 ttttaggtaa ccatctttcg ctgtccggga agtaatctcc gttttcgtat gcgctccctc
     6001 ctgcaatgtc caaaataagt tttgttgctc tgtctatagc aagcttattg ttgtaaggat
     6061 ggaggaattt gtcgtatctt tgaactgttt cgtttattat ttttagttta cgtgagtttt
     6121 ttcttacgct ggtttgatcg tagcttccgg aatcaagtaa aatgtcggtt gtactttgat
     6181 caatagcaac tgcctcaccc cccactatcc ccccgataat agttggcttg tcgtttgtgc
     6241 ttagtatagt tatgtcttca tctaaagtta tactttctcc aagaagtgta gttatgctct
     6301 ccccctttat cgcttttcta aaagttattt cgcgtgtttc cattttactt agatcgtgtg
     6361 cgtgcagggg ctgcccaagt tcaaccatta cataatttgt tatgtccact atgttgttta
     6421 taggaaccat tccgtactcg attagtcttt ttttaagcca ctcaggtgat tcttttactt
     6481 ttacattgta gattaccctt gtataaaaac gattaacaaa acctttgtct ttaactgtaa
     6541 ttttaacttt ttctttctcg ggtaacattg caagttcttg tgtgtgcatt tccggcatct
     6601 ttagagaaac attttcaaat gcagccagat cacgtgcgca tccgataata ccaagccaat
     6661 ctgctctgtc catcctatgt tccagatcta aaatttcgtt tccaaattct gatttttcta
     6721 ttggtttgtc caaaagcagc cccaaaaaag taaatgagct agctagctca aaggccgtct
     6781 tgtttatagc tacatattgt tttagccact ctaaaggtat tttcataggt atgccagctt
     6841 gcctccatat aaagtacgca aatcgctaac gccaaatttt tgcattacca gtctatctaa
     6901 tcccattcca aaagcgaagc ctgtataaat ttcagggtct attccacaca tttttagtgt
     6961 gttatagtga atcatccctg aacctaaagc ctcgatccac ccacgatatt tacagacatc
     7021 gcaaccttcc ccaccacaaa acttacattg catatctact cccacccccg gcgaaacctc
     7081 ggggtaatat ttgtacctga acctaacctt tgtgtcttca ccgtagaggt atttatgagc
     7141 ctgctccagc gtactcatta gatgttgtat agtgattccc ttgtcgacca caaaaccttg
     7201 atattggtta aagtacgctc catttgtcga acttagggtt tcgtttcgga aacatttccc
     7261 cggaacaact actctaatag gaggttcgta gtttgttaaa attcttgctt ccacagacga
     7321 ggtgtgtgtt cttaagacta tgtccatttc atctccgagc tgttgtataa aaagggtgtc
     7381 ttgcaaatct gtggcagggt ggccttcggg taaattaagc tttctaaaat tgaacagtgc
     7441 agtttctatt tcaggcccct cggctactga gaatccgcta tacctaaaaa attcatttag
     7501 ctgtcggatg acctgagttg tagggtgcaa aaagcctgca ttagtagctt tttcaggcaa
     7561 tgttaaatca agcaaagggt cgttaaggct ttcctctgcg agtaaattct ttttatcatt
     7621 tattttgttt ttctgttctt ctataagagt atttattttc ggtccatata gctttttttc
     7681 ttcagaagat agagaaggga tttttttaac taacattgta gtcagccccg ctgactttga
     7741 tagatattta aggtagactt cgttaatttt tgcctctgag gttgcagttt ccaaatcaag
     7801 ctcaaattgt ttttgtattt ctgttatgtt ttgctgcatc tttactatag tatgacagaa
     7861 aggcttatct ggcaatattc acataaggtc ttcttagatt aatttcaatt gtttaatttt
     7921 tattatgatt ggccttttgt cgggtaaaat tagcaaacat agtgaatcct ctaccattaa
     7981 gctaaaatat aggtgaaata aatattatgg ttagtacctt gcgcagagag aatttaatgc
     8041 ctcagagact gatggtggca taagttttca ttacgaatcg atagagctat tctgtaatgg
     8101 attaagccta gatgcaagcg aacctcttcc tgtacctagc ccgtgtgctg gaatacacgc
     8161 gtgtctagat gttgcagtaa atggttctgt ttcagtgact tcggtatgcg aaaggagctg
     8221 ctccttttat attcgggggg gggtggttct ccccggttat gaagcaaagc tctgagcaag
     8281 ggcagttaaa aagaacattg tgtttatcgg aataaggttt tttggttcta gttacaccta
     8341 tagttataaa gacgctgggg gatcagaagg ttatagatac gataaagggt agttttttga
     8401 tgctgatata gctctcgcgc aaaatactca agatctttat agagatagaa ataggagacg
     8461 ggctttaggt cgcgaagccc tatttatttg aggaaataat aagtaaaaac actagatttc
     8521 tgaaatttga tgcttgcttc cgcctctttt ttattggaga ataaagatta tgtatttctt
     8581 tgtgattaaa tatcaagaaa atgattaaga gggtgggtcg ccaaggtgag cccgtggagc
     8641 gacaagctaa tgaggcgcta aaaattctag gtattacgcg cctagttgag ccaaaagatc
     8701 cgcgagtgcc ctcgggtgat gctgttgtcg tgtctaagcg aataggggcc ttctgtgtca
     8761 tcgacagagt tggcggaagc gtaaatggcc cggaagttaa aggtgacagt cgggctgcag
     8821 gtatagtagc acgcgcagta gagaaaagaa ttgaagatgt ttcccgtcct attgatagta
     8881 caaagctcgc cgttgaattt ctgctaggtc ttcaaagtga tgctaagtct gagttagaag
     8941 ctgtgggcag atacgaacgt cattctgcag aaaaatatat tggcgctacc ctaaccgcag
     9001 ttctagtcgg taaagtcaaa attgctgtgt taaatgtagg agactgtcat gcagtatact
     9061 ttccaaggaa aggcaaaact tacgagccac caaagcatct aactcttgat gatacacgtt
     9121 tagttcagat tttggctgcg cgagagggcg cagaaaggga gttgttgtta aagcagcaaa
     9181 aagctatggc agctatggaa gacccctcat tgtatacgat tccaggaaat accctaccca
     9241 ttggcatgga tactttcttg cgaacgacgg ccgaagttac taatatggtg tcgtcgttcg
     9301 caagcactgg gtctgtagaa gggaatgtgg agatatacga taggagcgat ttggggccag
     9361 gtttattgct ccttatgagc gatgggactc atgaggtctg ttcggaccct gctctgctga
     9421 gggtccttaa tggattaaag cctcgccaga atcctgcaga agcagtaaga gacctagtta
     9481 tagcagaaag gaatcgttta ggtcaaggtc gttgtagata caagatcgac gatgccagta
     9541 atatatgtgt gacgctagga cagcccaagt tgtgaatgtc atgtttgcat agttaaaagg
     9601 tctcttatat aactttatac tgccaaatat cttccatggg cggcaaccgt agtaaaagtc
     9661 ccaaggcaat aaatgatgat cttagttaca actgtgcgtt tgaatctttc gtgcgttcct
     9721 tcattcttta ttacccatcc tgtttttttt gacgaacata aagttctatg gtaacttttt
     9781 gttaatattt ttttgttacc aataaattac agatatgcct cacgaacaaa ctgatacaga
     9841 gcatccagaa atgggagaat ttcgtataaa acattttaat gatcaagaaa ttctggatac
     9901 tgtctgtgtt gagggtgtgt gcagcgccct taagggtccc ggcactgcag ttgagcagtt
     9961 tgctctaccc gccgaggcag tggggttagt attggtacat gctcgtagta cacacggtca
    10021 tggcccaaga gatatggaag ctgatcctac aactgcggct gcgcgccaag cttcagaaat
    10081 ttttaaagag catcttgtac agcacttagc aaggcggccg cagtatggta atgattacga
    10141 tgcactttta gcttcttacc aagctattga acctgcttta ttacctcggg gtgataatct
    10201 tgcaatttcg gtagcctgtt tgttagtgcg gcaagaaggc gtggcatttt ctaatcttgg
    10261 agatacccgt gtgtacatgg ttaacccaaa aaccggcaca gcgcgttctc taactttaga
    10321 tactcctgcc acggcgcatc gaagagaata cgataaagca caagctttta gagatcaaga
    10381 tggggcacga attctagttg atgggccgag aaatccacga tctggctttg tgggtaatag
    10441 taattttcct ccagccattg aagttagtaa ccgaggcttt ctcggactta atgaagcatg
    10501 gttagtagtc actacccggg aaattaatag ttgtttaact gcgctcgatc ttgctcaatt
    10561 aagtggtgca ctcaatcaac ctgttctttt ccaaagggac gtattgaata gggttcaaaa
    10621 aggtgctgtg gctgttattg ctttaggaca ctaacatgca ccttgattcg tgctcaatac
    10681 tatgagttct ttgtgtttca cgttgtgtta attacctatg cgttatttag agatatgtta
    10741 ggtagggttt ttgcttctat ttaatggctt taactttgtt taccacttct tcaaaagctt
    10801 taggatcgtt tatagcaatc tcagaaagca tctttctgtt aagcactatg ccggttttag
    10861 gcagtatatg catgaatgtg ctgtaattta tttcatgctg ggtcagggct ccgcttatcc
    10921 ttgaaatcca gagttttctc atgtctcttc gtcttattct gcgtcctgca aaggcgtgct
    10981 cccctgccct taggactgcc tcattggctc gtctaaagag tttactacga gttcctctgt
    11041 agccttttgc tagttttatt atgcttctat gtttatttct ggctcttggg ccacctttaa
    11101 ctctagtcat cgtttattcc ttttctattg gagcaacttt tttcttactc accttttttg
    11161 aggtagttgc tttggagtta ctttttgttt ccgtagccgc cgcttcaacg ttctctgatt
    11221 ctactacatt taatgttatt ttggaaccgc ttttgcctag catttgtttg atgtttttta
    11281 tgtatccttt gctgactaca gcgcccaaac ctcttttgcg ggttttttta ttggctgacc
    11341 actttctctt taaatgtccg gttctatttt gtttttttag tataattccg tttttagtta
    11401 tttttaccct ttttgatagt gtttttcttg ttttcatatt tatcctttgt taagcactgc
    11461 tattatgttt ctgccttgaa ttctaatttg atcttcaagt tttgcaatgt ctgtaagagc
    11521 ggctacaaat ttttctactt tttctactcc tatttcagga tgtgcttgct ctcttccttt
    11581 catcactact gtgactttta cttggttacc gtccattaaa aactcacgtg cgcgatctgt
    11641 tttaactttt attgcccctt cgtctatgta agggccaaat ctaaactcct ttaacttgct
    11701 ttttttggct ttagctttta tttccgagct cttttttctc tccttataaa gatatttgtt
    11761 aaagtccaaa attttagcta ttggcggttt tgcgttttcg gtaattacta ccagatcaag
    11821 ttcttttgat cttgctactg ccagtgcctc tgatgtactt agaacaccta ggttttcgcc
    11881 atccccatcg tctactattc gtaattcagg gtatcttatt cggtcatttg ttttgtagta
    11941 tttgcttttt tcttgtttca ttcgttaaac gaaccaaaat taccataaca atgggtgttt
    12001 ggtcaaataa ttccttgtta gttagggttg gttttgggag agggggggcc tccgcggtat
    12061 ccgcggaggc ccccccccac caaacccccg ccaacaatcc cccgccaaca atcccctact
    12121 ctacttatcc tcccacaaag ccagcgactt actatccctt acatttgtga ctatcctaat
    12181 aaactcctcc acagaaacac cattaatctg ctcccctgtt cttagtctaa tgcttaccgt
    12241 attttgttcc tgctcccggc cgcccaaaat aagcatatat ggaagctttt gatgctgagc
    12301 ttctcttatt tttgactgca tggtgtcgtc tcgatcgttt acttttgatc ttagcttggc
    12361 ctttcggaga gacctatgca cttcgtttgc atagtcaagc accttttcgt tgataggtat
    12421 aatttcaacc tgttccggtg ccagccatac cggaaatgct cctccgaaat gctcgatcaa
    12481 gaagccgaca aagcgttcgt gagaacctag cggcgctcta tgaataactg cacatatttt
    12541 cttagaaccg tcggcagccg tgtattccaa attaaatctc tgtggcatat aaagatcaag
    12601 ctgcacggtt gatatagcga actctctgcc aatcgaggat tttacattaa agtctgtttt
    12661 cgggccgtaa aaagccgccc cgcctatatc atcgctcgat cttatacctg atttctctac
    12721 tgcccttttc atcattgcct cagcttcatc ccacaactct ttatctccgt gatatttttc
    12781 acgtttagtt tcgtcgggaa gaccaaaaac tattcgataa tcatctttag ttagccctag
    12841 agtttcatag tagtattcgt gtagcttcaa agaaattaaa aactcttcct ctgcttgttc
    12901 aagtgtgcag tatatgtggg catcgttttg agcaatagaa cgtaccctca ttagaccgaa
    12961 aagcgatcct gactgctcgt atctgtatac tgtgccatat tctgccattc tcatgggtaa
    13021 gtccctgtag gaacgaggtt gtgacttgta aatcatgtga tgatgaggac aattcatgcc
    13081 cttcagataa taatcttccc cgtcaatatc catcggactg tacatgtcgt ctttataata
    13141 aggtaagtgc cctgaagttt cgtatagggt gtgttttcct atatgaggtg tcgctactct
    13201 tacatagccc cactcctcct cagtctcctt ggcccatttt tcaatctctt ctctaataat
    13261 tgtgcccttt gcggtccaca agggcaggcc tgcccctaca tcgtcggcaa atgtaaataa
    13321 atccatctct ttccctatct ttcggtggtc acggtttttt gcctcttcca aaagggctaa
    13381 atgagcctcc agtgcctctt gagaatcaaa agcagtgcca tatatacgtt gaagcatctt
    13441 gttcttctcg tcacctttat aataggctcc tgctacgctt agaagcttga aagctttaac
    13501 ttttgaggta ttgtccacgt gcggtcctgc gcacaaatcc acgtcgtaat acttactttc
    13561 tggggaaccg aaaatgtaaa ctgatacttt ctcccccctg tctgcaattt cctgaat
//
LOCUS       DAOSWN010000012        12923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_3070_length_12923_cov_4.439074, whole genome shotgun sequence.
ACCESSION   DAOSWN010000012 DAOSWN010000000
VERSION     DAOSWN010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12923
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3070_length_12923_cov_4.439074"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="PLF29_01180"
     CDS             complement(<1..585)
                     /locus_tag="PLF29_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52585.1"
                     /translation="MPNSYIQHDLKKFEKFITLCSIFILTINSILNEKPTRSSVMAEE
                     LDNTPPITTIELTPSTPNGDNGWYTTPVNIKLTASDTLSGIKSINYKLNNGSWQTTTF
                     SDTQNLIQNSSFETPGATNTGIESWELSVDDPEVQASQYVKDYAPGYEVSSALLETTG
                     GTWHAISNFSAYASATPYDSMSASAWLKVDNAEGN"
     gene            complement(655..1965)
                     /gene="hisS"
                     /locus_tag="PLF29_01185"
     CDS             complement(655..1965)
                     /gene="hisS"
                     /locus_tag="PLF29_01185"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HXK52586.1"
                     /translation="MKDKLSTQPYKGARDFYPEDMLIQNYIFDTWKKVCKSFGYQEYS
                     FPILEPFEIFAAKTGEEIVNNQLLSLVDAGNRKIAIRPELTPGTVRMIAQRYKALTKP
                     LRWYMIGNNWRYEKPQKGRGREFYQLEVNIFGVGAVTADFEIFSIIIFLMQTFGATSN
                     MFRLKISDRRLISALLQDVLKLEENIQLQTRKFMDKKTKMPEKDFREGLREIGLNEEQ
                     TEKVVGFMNSNLEGLPSIIPQNTLYENKGYVAITEVFKLIENAGLKPYCEFDPSIIRG
                     FDYSDGMVYEVFDLSPDNRRSLFGGERFDKLIEIFADVELPSTGFALGDMTLLEFLVG
                     WNLLPHFDSAFDYLVTLWPVENKEESDSYFSISQSYANYLRSKGFKVLTWLEQNTKLE
                     KQLKYADKMGVKNVVLIGPEEIATKSAIIKDLETGKQKQDTIKL"
     gene            complement(2117..2644)
                     /locus_tag="PLF29_01190"
     CDS             complement(2117..2644)
                     /locus_tag="PLF29_01190"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HXK52587.1"
                     /translation="MHLLTANTDNFLFLNTNILSFSTAIKRINILKKQKIKCGYLSGI
                     FDMLHIGHINYLTIAKNYCDTLFVGMETTETLKAIKKVPTAINSYENRLQMMLQQKSV
                     DYVVRLDSSLNDYNSDASFEIQTQRYVQLNPDVIIIPDRTLVNFPRQLSQIEKAEKAL
                     GKEFEVVEILSLNQD"
     gene            2687..4069
                     /locus_tag="PLF29_01195"
     CDS             2687..4069
                     /locus_tag="PLF29_01195"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HXK52588.1"
                     /translation="MDIVHRSRRLAAGRAGKESFSGPVVYWMSRDQRVGGNWALLFAQ
                     KIALEARKPLLVVFCLVDSHLTSQRGDRNYSYMLGGLSLVREDLADLNIPFIILNGEP
                     PQKLLQFVEKFSVSALISDFSPIREGREWRNGVAKKINCSFYEVDTHNIVPAWVTSDK
                     QEYSARTIRSKLVKKLPDYLVPFKVVQRQNREVTEDFLTLIPNEIGFTNKILSVKSYE
                     GLSAVSPERLLQEFIDLKLDSYSRSRNNPLVDGTSNLSAALHFGHISSQGVILKIIEE
                     FGKTNKYDVQKYLENPKGNFFDEIIVRKELADNFCLYNKNYDNYLGFPNWAQKSLNAH
                     RGDKRDTVYTIRQLEQAETHDDLWNAAQVQMVSTGKMHGYLRMYWAKKILEWSSSPEE
                     AIISSIYLNDKYELDGRDPNGYAGIAWSIGGLHDRPWFDRPIFGQVRYMSYSGMKRKF
                     DVESFVKLYV"
     gene            complement(4354..7761)
                     /locus_tag="PLF29_01200"
     CDS             complement(4354..7761)
                     /locus_tag="PLF29_01200"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HXK52589.1"
                     /translation="MRETDHIGMLKLLYTLELTAIGFYDTISTVQNYMNYRPHFNNPF
                     WSIVFGRRICPPRKKMNKRKESIFSKIFSVFKSMTHRKRSKKFSRATVVSVIAVVAVV
                     SSATAAYSAGLISSLDISFGGRQTQGGPVLARSMEYGFTDVCGTHDVDGNNITFIPPF
                     NDVTDVRATNWNPGWLDLSKTMCNDRGYPVNQEGQCYTFPGYEGGTAYELDVNAPYCQ
                     AGSINEKAHAYVCDAEVTGGNPWLACGKSANYVYKALSGRSLSLGQLDEKKKIETGTG
                     LTCGSTVQMDIFNATCTTDMITQHYLVIDNGGDPSDPANWPLDPNGVPTFCLAKDAFV
                     WYTGSCEIEVQKRFVADNNGQPNPSQVKETAYEGENVWVEFTLDNTKGQYVYNETRTL
                     SDTLANNFRYEGQYQPENSDFTCTNSGQDVSCVFEGSIDKGETSKVYFKVNVLEGYLT
                     NEDCEVPNTVFLNDDTMTCTDDANDPSCSITDICLLRVEEEDFDLDIVKKFYGDSNDK
                     PSQNEITQVKVDDKAWIKFRVTNTGEGAYFGSWQDGGVVRANPMLINDDLNEVDNNVL
                     EYTGKLTTDYDGFNYNQCNFITNNSELVCNITTVDLQPDAKFDIYAQVKVTDMPDNNS
                     NGICPANSVKYTNHASLEFTNLAGETQDCSNPFNASDPQCTEDYLCMMEDIVVEEPEL
                     TIEKVNLDGNKVYKPGEQIRYEIRIANVGKGDATDVAFNDAVPSEIDYATVALSVSPE
                     SFRNDFVYNESTGVIRSDSDGFDLDAGGRITLKVVAKIKSDIEKSEDDEIYNQACITD
                     QVAGDFEKVYCDDVTNTLVWDDDIVPVPGIDIKKDIDISKSTGDDTTYEKGDDIAFKI
                     SVRNTGETELANVVISDKVPAYTEYNAEKSKAYGFDWSCSNGIGAGKDCTLDIGTLKI
                     GETKVVYFVSTVVDYDNTGSHNLDSENIVCIVAGMPKDEKVCDDADFDLKVIEDEEEE
                     EKEIKIDVDKEVRIKGDDEWLDKVTNVKKDDVLEFRIRVKNEGKDELDNMKMEDFLPD
                     ELKKIGGSGLTEYWENFKEGKTKTFIIEVKVRDSEFDKENVDKCVKNKVEVYYDGDEV
                     GSEDAKVCYVNKEITELPETGAETNLALTVLGLTSAVAGIRVRRKLS"
     gene            8043..9152
                     /gene="ychF"
                     /locus_tag="PLF29_01205"
     CDS             8043..9152
                     /gene="ychF"
                     /locus_tag="PLF29_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008427707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HXK52590.1"
                     /translation="MSLSIGIVGLPNVGKSTLFNAMLKRQVALAANYPFATIEPNIGI
                     VDLPDERLNKLAEVVLKEFRVSPERIVPATVKFVDIAGLVEGASAGEGLGNQFLGHIK
                     DVSAIVQVVRDFSDENVIREGSIEPKSDIEVINTELMLADLQTINSRIERHAGVLKKE
                     KTLENLHKQELYLSIKVALEKGASLLSLGFSSEDLELLKDLHLLNLKPKIYVLNCDED
                     AVADGTSIPSLDFLESHDSHVVKLCAKIESELCSLSDEDRSAYMRELGLSESGLDVVI
                     KTGFALLGLQTYFTAGPKEVRAWTIKKGDKAPKAAGVIHTDFEKGFIKAEVFSFEDLV
                     MYGSFKECKARGVARIEGKDYIMQEGDVVEFRVNA"
     gene            9231..9515
                     /gene="rpsF"
                     /locus_tag="PLF29_01210"
     CDS             9231..9515
                     /gene="rpsF"
                     /locus_tag="PLF29_01210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HXK52591.1"
                     /translation="MKQYELMTITDIDLGEDGSRDISNKIKDLIASKKGKVLDSDFWG
                     KRRFAYEIDHKSEGFYEVMNFELPADAVSALKSELNYITGLVRYLVTATN"
     gene            9545..10009
                     /locus_tag="PLF29_01215"
     CDS             9545..10009
                     /locus_tag="PLF29_01215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HXK52592.1"
                     /translation="MTARDVNKVILIGNLTRDPEVRYTPQGTAVANFTIATNRSWSID
                     GEKKEAVDFHNVVAWNKLAELCGQLLTKGTKVYVEGRLQTRNWVGDDGVKRYRTEINI
                     DEMIVLISKGSLAEEDSYDETHAVGEERSEESVDGVDIDDLLNELEENSVEE"
     gene            10011..10247
                     /gene="rpsR"
                     /locus_tag="PLF29_01220"
     CDS             10011..10247
                     /gene="rpsR"
                     /locus_tag="PLF29_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008636608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HXK52593.1"
                     /translation="MAKRTKQKQVETECYFTKTNTTPNYKDVLILRRFISDRGKIIPQ
                     GRSGVTAKNQRVLSQEIKKARYMALLPYTDQHSI"
     gene            10363..11388
                     /locus_tag="PLF29_01225"
     CDS             10363..11388
                     /locus_tag="PLF29_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52594.1"
                     /translation="MPVLSFYEGYSELTFVYSESDKSKVGNELLELDAKPEIFKLPFV
                     YSANLYSCSFSKEQFYKEVFSLVYSQLGITKDQLPGLEFYASGYIKVPDSLPLKPKKS
                     FCVSDFIHEPYLFVGSDFLIQQGCFTSLLPVSYLTQYANFYSNYSIYPGMKLTEPRDI
                     VLTDQLVRDSMYFSSLSSSLNLKPKKPVTKKAVFTGERLSNYEENKALSLLLMLDLLI
                     YPGMYQLQLDKSNLLPALFALDITNFGLLSIGSAIVSPGYNEVLFKSEAASQQFFEIS
                     EDSIFILPLDAEEQGVVAVKSELGTMEYEVKGGEVGLVFDARRRDMGPVYKDVYINMI
                     EQNLLRI"
     gene            11390..12382
                     /locus_tag="PLF29_01230"
     CDS             11390..12382
                     /locus_tag="PLF29_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52595.1"
                     /translation="MAYYIPVYRKMNLHRESLVRRELPIEGDLIVKVGDSVLPFTKIG
                     RATISKDFLVLPPKIKIQRSMLPKPLIYEGEVLGRAGFRSFKAPFNGYFEEDSINGLV
                     YKREKQDYWMLAGVWGVVSEVVGGRSVTIKAQVIDINFSAACGTDHMAELIVFPNPSK
                     LLDLEYLQNFSKGIKGKIVYVGDFLRKELVLKAIELKVGALIGGSIEKDIFNLAKSRG
                     LGVGITTGFGQLQTPSKVFDFLKTVSNRHVFIDVSDGKLTIPIPSEFNTSSFAENGES
                     ICEVKKEVPVLVLQSPYFGREGKVDDIQNDKIYVKLNESEEVHEINNSNLLALL"
     gene            12387..>12923
                     /locus_tag="PLF29_01235"
     CDS             12387..>12923
                     /locus_tag="PLF29_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HXK52596.1"
                     /translation="MQQNRKSSFQYIFTIFIVSVLMFFLGLYFGQRGYSVNLVNNPPK
                     IEILNKRPISSEVDFSLFWEVWDEVNASHLERPLDPEKLLYGAISGMVDAIGDPYTSF
                     LPPELNESVNASLDGKYEGIGAELGFREDQLIIVAPFEGSPAIKSGVRAGDKIIKING
                     EMTVGLSITEAVAKIRGAA"
BASE COUNT         3545 a         2429 c         2731 g         4218 t
ORIGIN      
        1 atttccttcc gcattatcaa cttttagcca agcagaagcg ctcatgctgt cgtaaggggt
       61 cgcagatgcg taggcagaaa aatttgatat tgcatgccat gttccgcctg tagtttctaa
      121 taaagctgaa gaaacttcat atcctggggc atagtcttta acgtattgcg acgcctgaac
      181 ctcaggatcg tccacagaca actcccaaga ctcgattcct gtatttgttg cacccggtgt
      241 ttcaaaagat gagttttgta ttaaattttg tgtatcacta aaagtagttg tttgccatga
      301 cccgttattg agcttgtagt ttatagattt gattccactt agggtatctg acgccgtaag
      361 ttttatgttt accggagtgg tgtaccagcc gttatctcca ttcggcgtgc ttggtgtgag
      421 ctctatggtt gttatgggcg gggtgttgtc taactcttct gccatcacag aagaccttgt
      481 cggcttttca ttcaagatag aatttattgt caggataaag attgagcata gagtaataaa
      541 cttttcaaat tttttgaggt cgtgttgaat atacgagttg ggcattaatt aaattctaga
      601 tacccgaacc attaatgtca aagattatta ggtattggtt ttagtttttt tatcctacag
      661 cttgatcgtg tcttgttttt gttttccggt ttcaagatct tttataatgg cggattttgt
      721 agctatttcc tcagggccaa ttaaaactac gtttttaaca cccattttat cagcatattt
      781 tagttgtttt tccagtttcg tattttgttc taaccaagtt agaaccttaa agcccttaga
      841 tcttaaataa ttagcatagc tttgagatat agaaaaataa gaatcgcttt cttccttatt
      901 ttcaacaggc cacagagtta caaggtagtc aaaagcgctg tcaaaatggg gcaaaagatt
      961 ccaccctact aaaaattcca acaaggtcat gtctcctaag gcaaagccgg tagatggaag
     1021 ttccacatca gcaaatattt caataagttt atcaaaacgt tctccgccaa atagggacct
     1081 tctattatcc gggcttaggt caaaaacctc gtagaccatg ccgtcgctgt aatcaaagcc
     1141 tctgattatt gaggggtcaa actcacagta aggctttaat ccggcatttt cgatcagttt
     1201 aaatacttcg gtaattgcaa cgtatccctt gttttcatat aaagtatttt gaggtattat
     1261 gctcggtaat ccttcaagat tagagttcat aaagccgacc actttttctg tctgctcttc
     1321 gtttagtcct atttctctaa ggccttctct aaagtccttc tcaggcattt tagttttctt
     1381 atccataaat tttctagttt gaagttgtat attttcttcg agttttaaaa cgtcttgaag
     1441 tagcgccgaa ataagtcttc tatcgcttat ttttaatcta aacatattag aagttgctcc
     1501 aaaagtttgc attaggaaaa ttatgattga gaagatttca aaatcggcag taactgcccc
     1561 caccccaaaa atgttaactt ctagttggta gaattctcgg ccccggcctt tttgtggctt
     1621 ctcatatctc caattgttgc ctatcatgta ccatcttaga ggcttagtaa gagccttgta
     1681 tcgttgcgca atcattctta ctgttcctgg agtaagttcg ggtctgatcg ctatcttgcg
     1741 gtttcctgca tcaactagtg aaagcagctg gttgtttact atttcttctc cggtttttgc
     1801 cgcaaagatc tcaaaaggtt ccagaatggg aaagctgtat tcttggtatc caaagctttt
     1861 gcagaccttt ttccatgtat cgaagatata gttttgaata agcatgtcct caggataaaa
     1921 atctctagct cctttgtaag gttgagttga taatttatct ttcatattta atccttcttt
     1981 aattttgcag actgaagttt gtgtcgagtg ctacaatttt gcctgctttc tttattatat
     2041 tacataaaat tagaataatt tacaccgaag cattctataa ttaattttca gagcaagctg
     2101 attctgagaa tagccactaa tcctgattta ggcttaagat ttctacaacc tcaaattctt
     2161 tgccaagcgc tttttccgct ttttctattt gcgacagctg tcttggaaag tttacgaggg
     2221 ttctatccgg aatgattatt acatcgggat ttagctgtac atatctttga gtttgaatct
     2281 caaacgaggc gtcggagttg tagtcattga ggctagaatc caacctcact acgtaatcga
     2341 cgcttttttg ctgaagcatc atttggagcc tattctcgta agaattgatg gccgtgggaa
     2401 cttttttgat tgctttgagg gtttcggttg tctccatgcc gacaaagagt gtgtcgcagt
     2461 aatttttggc tatcgttaga tagtttatgt gcccgatatg tagcatatca aaaatacccg
     2521 agaggtagcc acatttgatt ttttgttttt taagtatgtt tatacgtttt atagctgttg
     2581 aaaacgacag tatgtttgtg tttaaaaaaa gaaaattatc ggtattcgct gtaagcaagt
     2641 gcatgttttt attttatcaa atcggctttt attgtatgct ttagaaatgg atattgttca
     2701 tagatcgcgt cggttggcag caggcagggc agggaaggag tctttctccg gacctgttgt
     2761 gtattggatg agtagagacc agcgggtggg gggcaactgg gccctgttat ttgctcagaa
     2821 aatagccctg gaagctcgca agcctttatt ggtggtgttt tgtcttgtag actcgcattt
     2881 gacttctcag cgaggcgata ggaactattc ttatatgctt ggcggactgt cgctggttcg
     2941 agaagatctt gcagacctaa atataccctt tattatccta aatggagagc ctccccagaa
     3001 attactgcag tttgtcgaga agttcagcgt ttctgcttta ataagcgatt tttcccctat
     3061 cagagaaggg agggagtggc gaaatggtgt agcaaagaag attaattgct ctttttacga
     3121 agttgacaca cataacattg ttcccgcatg ggtgacgtcg gacaaacagg aatactcagc
     3181 aagaaccata agaagcaagc ttgtcaaaaa gctgccagac tatcttgtac catttaaggt
     3241 tgtacaaagg cagaataggg aagttaccga agacttccta actttgatac cgaatgagat
     3301 tggttttaca aataaaatat taagtgtgaa atcttacgaa ggtttaagtg cagtttctcc
     3361 cgagaggtta ttacaggaat ttatagactt gaaattagac tcatactcta ggtctagaaa
     3421 caacccttta gtagatggta cctctaatct gtcagccgca cttcatttcg gccatatctc
     3481 atctcaaggt gtaattctaa agattataga ggagtttgga aaaacgaaca aatatgatgt
     3541 tcaaaagtat cttgaaaacc ccaagggtaa ctttttcgat gaaattatag tgagaaagga
     3601 gctggcggat aatttttgct tatataataa aaactatgac aattatctcg gctttcccaa
     3661 ttgggcgcaa aaatctctga acgcgcaccg tggcgataaa cgagatacag tttatactat
     3721 aaggcagctg gagcaggctg aaacacacga tgatctttgg aatgcggctc aggttcagat
     3781 ggtttcgaca ggaaaaatgc atggatattt gagaatgtat tgggcaaaaa aaattctcga
     3841 gtggagctcg tctccggaag aggctattat ttcgtctatc tatttaaacg ataaatatga
     3901 gctagatgga agagatccaa atggttatgc cggcattgcg tggtcgatcg ggggactgca
     3961 tgataggcct tggttcgacc ggccgatttt cggacaagta agatacatga gttatagtgg
     4021 aatgaaaaga aagttcgatg ttgaatcctt tgtaaagctt tatgtttgaa atcaaccacc
     4081 accagcttat gcaggtggtt tgctgtaatt tctttgcact gcaaagcaca acgtgttggg
     4141 caaactacac cgactagctt acgcaagtag tattaagtaa cgaaactata aaactagggg
     4201 acgcagtctc ttgagccttg ttggtttttg tcgttatttt tttttggtat ttagaagagg
     4261 gaagttttgc agagaaaaat aaagtggggg gggctttggg gagaagaggg ctacggcctt
     4321 cggccgtagc ccctcacttc tctacagtgt gagttaggaa agctttcttc taactcttat
     4381 acctgctact gctgaggtta agcccagtac agtaagtgca aggttagttt cggctcctgt
     4441 ctctggaagc tcagttatct ctttgtttac gtaacaaact tttgcatctt cgcttcctac
     4501 ctcatctccg tcgtagtaga cttcgacttt gttctttaca cacttatcta cgttctcttt
     4561 gtcaaattca gagtctctga ccttgacttc gattatgaag gtcttggttt ttccttcttt
     4621 gaagttttcc cagtattcag tcagtcctga tccgcctatt ttcttaagtt cgtcaggtag
     4681 gaagtcttcc atcttcatgt tgtcaagttc gtctttacct tcgtttttaa ctctaattct
     4741 gaactctaag acgtcgtctt ttttgacatt ggttacttta tctaaccatt catcgtcgcc
     4801 cttgattcta acttctttat caacatctat ctttatttcc ttttcttctt cctcttcatc
     4861 ttctattact ttcaagtcaa aatcagcgtc gtcacatacc ttttcgtctt taggcattcc
     4921 ggcaactata catacgatgt tctctgagtc taaattgtga cttcctgtat tgtcgtagtc
     4981 gacaactgtg gacacaaaat atactacttt agtttcaccg atttttaatg tgcctatatc
     5041 taaggtacag tcttttcctg cacctatgcc gttgctacat gaccaatcaa atccgtacgc
     5101 tttactcttt tctgcattat actcagtata tgcaggtact ttgtcgctaa ttaccacatt
     5161 ggccaattcg gtttcaccgg tgtttcttac gcttatctta aaggctatat cgtcaccttt
     5221 ttcgtatgta gtgtcgtctc cggttgactt agagatatct atatcttttt tgatgtcgat
     5281 tcccggtaca ggtacaatat cgtcatccca aaccagcgta tttgttacgt cgtcgcagta
     5341 tactttttca aagtcccctg ctacttgatc tgtaatacat gcttggttgt agatttcatc
     5401 atcttcagat ttttcgatat ccgatttgat tttagcaact acttttaatg taattcttcc
     5461 gcctgcatct aaatcgaacc cgtctgagtc tgatctaata acccctgtag attcgttgta
     5521 cacaaaatca tttctgaagc tctcaggaga tactgacaga gcaacagtag cgtagtctat
     5581 ttcactaggt actgcgtcgt tgaaggcaac gtctgttgca tcgcccttac cgacatttgc
     5641 aattctgatc tcgtatctta tctgttcgcc tggtttgtat actttgttac catcaaggtt
     5701 gaccttctct attgttagtt caggttcttc taccactatg tcttccatca tgcaaaggta
     5761 gtcttcagta cactgaggat cgcttgcatt aaatgggttg ctacaatctt gagtttcacc
     5821 tgcaagattt gtaaactcta ggcttgcgtg gttagtatac tttactgaat ttgctgggca
     5881 aattccattg ctgttgttgt caggcatgtc tgttacttta acctgtgcat atatatcaaa
     5941 cttagcatca ggctgtaagt ctactgtagt tatgttacat acaagctcgc tgttatttgt
     6001 aataaagtta cactggttgt agttgaaacc atcgtaatcg gttgtcaatt tacctgtgta
     6061 ctcaagtaca ttgttatcca cttcatttag gtcatcgtta atcaacatcg ggttagctct
     6121 aactactcct ccgtcttgcc atgatccaaa gtacgcacct tctcctgtgt tagttacacg
     6181 gaattttatc catgccttgt cgtctacttt gacttgggta atttcattct gtgaaggttt
     6241 gtcgttacta tctccataga atttttttac tatatcaaga tcaaagtctt cttcttcaac
     6301 tctaagcaga caaatgtctg taattgagca tgaagggtcg ttggcgtcgt ctgtacaagt
     6361 cattgtgtcg tcgtttaaga atactgtgtt aggtacttcg cagtcctcgt ttgttaggta
     6421 tccttcaagc acgttaactt tgaaatatac tttgcttgtt tcgcctttat ctatagaacc
     6481 ttcgaataca cagctgacat cctgcccgct atttgtacaa gtaaagtcgc tgttttcagg
     6541 ctgatactgc ccttcatatc taaagttatt ggctaatgtg tcagacagtg ttcgtgtttc
     6601 gttgtataca tactgtccct tggtgttgtc caaagtgaat tcgacccaaa cattttcgcc
     6661 ttcgtaagct gtttctttta cctgtgaagg gttaggctgc ccgttattat cggcaacaaa
     6721 tcttttttga acttcgattt cgcaagaacc tgtgtaccaa acaaaggcat ctttagctaa
     6781 acagaaggta ggaaccccgt ttggatccaa aggccagttt gcaggatcag aaggatctcc
     6841 accattatct attactaagt agtgttgggt aatcatgtcg gttgtacagg ttgcgttgaa
     6901 tatatccatc tgtactgtac ttccgcatgt aagtccggtt ccggtttcaa tctttttctt
     6961 ttcgtctaat tgacctaagc ttaaagatct tccacttaat gctttataca catagttagc
     7021 tgattttcca caggctaacc aagggttgcc tccggtgact tcagcatcgc aaacgtacgc
     7081 atgtgccttt tcgttaatgc tgccggcttg gcaataaggt gcattaacat ccagttcata
     7141 tgctgttccg ccttcgtatc ctgggaatgt ataacactgg ccttcctgat ttacagggta
     7201 gcctcggtcg ttacacatag ttttacttaa atctaaccat cccgggttcc agttggtggc
     7261 tctaacatct gtgacgtcat tgaacggagg tataaaggta atgttattac cgtctacgtc
     7321 gtgtgttcca caaacgtctg tgaagccgta ttccatgctt ctcgccaata ctggcccgcc
     7381 ttgtgtctgt cttcctccaa agcttatatc tagtgagctg ataagcccag cagagtaagc
     7441 tgcggtggct gaggacacta ctgcaacaac agcaatgacg ctcacgacag tagcgcggct
     7501 aaatttttta cttctttttc tgtgagtcat agatttgaac acagagaata ttttagaaaa
     7561 aatactttct tttcttttat tcattttttt acgtggaggg caaatcctac gtccaaaaac
     7621 tatagaccaa aaagggttat tgaaatgggg cctatagttc atataatttt gaactgttga
     7681 gattgtatca tagaacccta tagcagtcaa ctctaaggta taaagcaatt tcaacatccc
     7741 gatgtggtct gtttctcgca tttcttattc agaaatttat tgttatatag ttattcccaa
     7801 cttatttgta gatgtctttt tggggctctg ttgcagcgtt ctcacagatt gtctctctgc
     7861 gtctagttcc cggagcttgg cgggcgcttg tggttttatg gcacccctca ccctcggtct
     7921 tatggcacat atttgaaata tttgcttgta ataatgtagt acagaagttg aacttttaag
     7981 ttgggccggg gcactaagta agtactttga actcttctct ttatgaagta aaatacctct
     8041 gtatgtcttt atcaataggg atcgtaggtc ttccaaatgt aggtaagtca actttattta
     8101 acgcaatgct taaaaggcaa gtagcgctag cggctaatta cccttttgca accattgagc
     8161 caaatattgg tattgtagac cttcctgatg aaagacttaa taaactggct gaggttgtac
     8221 ttaaggaatt tagggtgtct cccgaaagaa ttgtgcccgc gaccgtaaag ttcgttgata
     8281 tagcgggcct tgttgaaggt gcctctgccg gagaaggatt ggggaatcag tttctggggc
     8341 atataaagga cgtgagcgct attgtacagg ttgttaggga tttttcagat gagaatgtaa
     8401 tacgagaggg ctcgatagag ccgaagtctg acattgaggt tataaacacg gaactcatgt
     8461 tggccgacct ccagactatt aatagtagaa ttgagcgcca tgcgggtgtg ttaaagaagg
     8521 aaaaaactct tgaaaatctt cataagcagg agctgtatct atctatcaag gttgctttgg
     8581 agaaaggagc ttcactttta tctttaggtt tttcatccga ggatctggag cttttaaagg
     8641 atttacattt attaaattta aaacctaaaa tatatgttct gaactgtgat gaagatgctg
     8701 tagcggacgg cacatctatt ccaagcctag attttttgga aagtcacgat agtcatgttg
     8761 tgaagttgtg tgcaaagatt gaatccgagc tatgttccct ttccgacgag gacagaagtg
     8821 cttatatgcg ggaactgggg ttatctgaga gcggcttgga cgtagttatt aagacaggct
     8881 ttgctttgtt gggacttcag acttatttta cagctgggcc gaaagaggta cgtgcttgga
     8941 ctataaaaaa aggagacaag gctccaaaag cggccggcgt gattcacaca gacttcgaaa
     9001 agggctttat taaggctgag gttttttcat ttgaggattt ggtcatgtac ggtagcttca
     9061 aggagtgtaa ggcccgtggg gtagctcgca tcgagggcaa ggattatatt atgcaagaag
     9121 gggatgtagt ggaatttaga gttaatgctt aggatactat ctttaatcta gttagggtag
     9181 aatatctata gtagacattt aagaaatctt ttgtataatc aatttgcgtt atgaaacaat
     9241 acgaattaat gacaataaca gatattgatc ttggtgaaga cggctcaaga gatatatcca
     9301 ataaaattaa agacttaata gcttccaaaa aaggtaaagt cctcgatagt gatttctggg
     9361 gcaaaagacg atttgcttac gaaatagatc acaaatcgga gggtttttat gaggttatga
     9421 atttcgagct acctgccgat gcagtttcag ctttaaaatc agaattaaat tatattaccg
     9481 gcttggttag gtatttagtt acagcaacaa attaacagca tctacatatt gctaaggagg
     9541 tcaaatgact gcaagagatg ttaacaaagt tattttaatc ggaaatctta caagagatcc
     9601 tgaagttaga tatacccctc agggtactgc tgttgcaaat tttactattg caacaaatag
     9661 atcctggtcc attgatggtg agaaaaagga agctgtagat ttccataatg ttgtagcatg
     9721 gaacaagttg gccgaacttt gtggccagct tcttacaaaa ggtacaaaag tctatgttga
     9781 aggcagactt caaactagaa actgggtagg cgatgacggt gtaaaaagat atagaacaga
     9841 gattaatatt gatgaaatga tagtgctaat ctcaaaagga tctttggccg aagaagattc
     9901 ttacgatgag acgcatgctg taggagaaga gaggtccgaa gagtcagtag atggagtcga
     9961 tattgatgat ttattgaatg aattagaaga aaattcggtt gaagaataat atggcaaaaa
    10021 gaacaaaaca gaaacaagta gagactgaat gctacttcac aaaaacaaat acaactccta
    10081 actataagga tgtactaata cttagaagat tcatttccga taggggcaag attatacctc
    10141 agggtaggtc cggtgtaact gcaaagaacc agagagttct ttcccaggag ataaaaaaag
    10201 ctagatacat ggctttactt ccttataccg atcaacattc gatatagttg ctcgatcttt
    10261 tcgcaagata gatgatttat gggggccccg atctatgatc ggggcccctc aatttttctt
    10321 gatttgcttt caacacacag cgtcaaatgt tattattttt acatgcccgt tttgtcattc
    10381 tacgaaggct actctgagct gacatttgta tattctgaat ccgataaatc caaggtgggg
    10441 aacgagttgt tggagcttga cgctaagccg gagatcttta agttgccctt cgtttacagc
    10501 gcaaatcttt attcctgctc gttttcaaaa gagcagtttt ataaggaagt tttttctttg
    10561 gtttatagtc agttgggaat tacaaaagac caactacccg gtctagaatt ttacgcttcc
    10621 ggttatatta aggtgcctga ttctcttcct cttaaaccga aaaagtcctt ttgcgtatcc
    10681 gactttatac acgagccata tttatttgta ggttcggatt ttctaatcca acaaggctgc
    10741 tttacttctc ttttgcccgt gtcctatttg actcagtacg caaacttcta ttctaattac
    10801 agtatctatc cgggcatgaa actaacagag cctagagaca ttgttttaac agaccagctt
    10861 gtaagagatt ctatgtattt ctcttcctta tcttcttcac taaacttgaa accgaaaaaa
    10921 cctgtaacaa aaaaggctgt tttcacaggc gagaggcttt ccaactacga ggaaaacaag
    10981 gccctttcgt tgttacttat gcttgatttg ttgatatatc ctggtatgta tcaactacag
    11041 cttgataaat caaacctttt gccggcactt tttgcgttag atattaccaa tttcggccta
    11101 ctttcaatag gttccgcaat agtatccccg ggttataacg aggtgttatt taaatctgaa
    11161 gccgcaagcc agcaattttt tgaaatttcg gaagattcaa tttttatatt gcctctggat
    11221 gccgaagagc agggggttgt ggcggttaaa tcggagttag gaactatgga atatgaagtt
    11281 aagggcggcg aggtgggttt ggtgtttgac gctcgtaggc gagatatggg gccggtttat
    11341 aaagatgtgt atattaacat gattgaacaa aatttattga gaatataata tggcttatta
    11401 catacctgta tacagaaaaa tgaaccttca cagagaaagc ttagtaagaa gagaacttcc
    11461 tattgaaggt gatttgatag tgaaggtggg ggattcggtt ttgcctttta cgaagatcgg
    11521 ccgtgcaacc atatcgaaag attttctggt tcttcctcct aaaataaaaa ttcagaggtc
    11581 aatgttgccg aaaccattaa tttacgaagg cgaggtgctc ggtagagccg gctttaggag
    11641 ttttaaagcc ccgttcaatg gctatttcga agaagattct attaacggcc ttgtttacaa
    11701 gagagagaag caggattatt ggatgttggc cggtgtttgg ggggtagtta gtgaggtcgt
    11761 tggtggccga agtgtcacta ttaaagccca agttattgat ataaattttt ctgccgcttg
    11821 cggcacagac catatggccg aattgatagt ttttccaaac ccgtcaaagc ttttggattt
    11881 agaatattta caaaattttt caaaaggaat taagggcaag atcgtatatg ttggtgattt
    11941 tttacgtaaa gagctggtac tcaaggcaat tgaactaaag gtaggcgcgt tgattggggg
    12001 cagtatagag aaagatattt ttaacttggc gaaaagcagg ggtttaggag ttggtataac
    12061 gaccggattt ggccagctac aaacaccaag caaagttttt gattttctta agacagtctc
    12121 caatcgacac gttttcatag atgtttcgga tggcaaatta actattccaa ttccgtccga
    12181 attcaatacc tcctcttttg cagagaatgg agaatctatt tgcgaggtta aaaaagaggt
    12241 tcctgtgtta gttttgcaga gcccatactt tggaagagag ggaaaagtag acgatataca
    12301 gaatgataaa atttatgtta aactcaacga gtcagaagag gtacacgaga ttaataattc
    12361 caaccttctg gccctcttat aaagctatgc aacagaatcg aaaatcttca tttcaataca
    12421 tattcaccat attcatagta agtgtattga tgtttttttt agggctgtat tttggccaaa
    12481 ggggttattc agttaacctg gtaaataatc ctccaaaaat tgagatttta aataaacggc
    12541 cgataagttc tgaagtcgat ttttcgttgt tttgggaagt ttgggacgag gttaacgcct
    12601 ctcacctaga aagacctctt gatcccgaaa aactccttta cggagcaatt tcaggcatgg
    12661 tagatgcaat tggcgatcct tatacttctt ttttgccgcc ggagttgaat gagagtgtta
    12721 acgcttcgtt ggacgggaag tatgagggaa taggggccga gttaggtttt cgagaagatc
    12781 agctcatcat tgtagctcct tttgaaggct ctcctgcgat aaaatccgga gtacgagccg
    12841 gagataagat tattaaaatt aacggcgaaa tgactgtggg tttgagtatt accgaagcag
    12901 tagcaaaaat acgaggcgca gcc
//
LOCUS       DAOSWN010000013        12900 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_3075_length_12900_cov_5.003815, whole genome shotgun sequence.
ACCESSION   DAOSWN010000013 DAOSWN010000000
VERSION     DAOSWN010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12900
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3075_length_12900_cov_5.003815"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="PLF29_01240"
     CDS             complement(<1..696)
                     /locus_tag="PLF29_01240"
                     /inference="COORDINATES: protein motif:HMM:NF020118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HXK52597.1"
                     /translation="MATTEFKAALIQVATERGISAEDVIESIKSALISAYKKDYGADL
                     TEEQIEEITVIVDPDTGEAKIIKDKKDVTPPGFGRIAAQTAKQVILQKIRESEKKVLF
                     DEFKDKIGILITGQIFRIENGLIILDIGKAQGVLPQSEQVENEHYRINQRLKVYVKDV
                     RTGPRGTEVILSRSDAELVRRLFEQEVPEVSSGVVEIKAIAREAGSRTKIAVSSNDAK
                     IDPVGSCVGQKGVR"
     gene            complement(918..1589)
                     /locus_tag="PLF29_01245"
     CDS             complement(918..1589)
                     /locus_tag="PLF29_01245"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXK52598.1"
                     /translation="MTTIYLVRHGENLANITRELSYKHIDYELTNKGHFQAAQTATAF
                     DNITIDAIYSSPLKRAMQTAEYIAKRKKLIPKILENFREINMGKVEGMKPNEESWKQY
                     FLITHKWYMGEEDISFPEGENYSQLVNRFATGLLEAIKKTLHGNIAVIGHAGIFAAGV
                     IHLCKIENKKEFSAIQNHNCSISELSVTQNNQNLSYSLVKWADSSHLTGSAAKLVKGM
                     PESSS"
     gene            complement(1726..2640)
                     /locus_tag="PLF29_01250"
     CDS             complement(1726..2640)
                     /locus_tag="PLF29_01250"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HXK52599.1"
                     /translation="MEKRDYYEILGISKSASPEEIKSAYRKLAREHHPDMVKDGDKAA
                     AEKRFKEINEAYQILSEPDKRKMYDQFGHEGAKYRNTGQGFSGGGAGGPFSYTYTTGG
                     AGFDGIDPFDIFEQFFGFRGFGGRRQNKGKNLYYELHVAFEDAVHGAKKDITTEAGKI
                     TIKIPQGARDGIELRFAGKGMPGPAKDIPNGDLFITLRIATPKIFQRAGDNLGTLLKI
                     DFIQAILGDEVPVDVIDTSEKTGMGHVKLKIPAGTQNGAQFRLKNRGMPKLQGTGQGD
                     LIVQVEVITPKKVSKKQKQLLEEYRKNS"
     gene            complement(2729..4609)
                     /gene="dnaK"
                     /locus_tag="PLF29_01255"
     CDS             complement(2729..4609)
                     /gene="dnaK"
                     /locus_tag="PLF29_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HXK52600.1"
                     /translation="MGKIIGIDLGTTNSAMAVMESGQPKIIPNSEGMNTTPSIVNPTA
                     NLVGVAAKRQQVVNPKNTIFSIKRLIGMKFNDPEIDTLKKNYPYTIVEGKNGMAAVEV
                     DGKTFTPQEISAMILRKLKADAEKYLGQEVTEAVITVPAYFNDAQRSATKEAGQIAGL
                     EVKRIINEPTAASLAYGLDKKSNEKIVVYDLGGGTFDVSVLEVGDGVFEVKSTNGDTH
                     LGGDDFDVCLINWLLEEFKKETGIELTQDKQAMQRLKDAAEKAKIELSSTLETEVNIP
                     FITADANGPKHMNIKLTRSKYEQLVEELINKTLKPIENALKDASLDKGQIDEIVMVGG
                     MTRTPKVNEVVKKSFGKEPHKGVNPDEVVAIGAAIQGGVLGGDVKDVLLLDVTPLTLG
                     IETLGGIMTPLIKRNTTVPTAANQIFSTAADSQTSVEINILQGEREMAIDNKSLGRFI
                     LDGIPPAPRGVPQIEVTFDLDASGIIHVSAKDKTSGKEQKIKIEGGTGLSQEEIDKMV
                     KDAQLHAEEDKKKKEQIEARNIADNMCHAAEKALKDSEGKLTEEIVSDITAKVKGVRD
                     VLSTASTEELKQKTEELSSAMQKIGEHLAKQSQTGTEEQGNDPKREGPEDEVTEGEVV
                     QE"
     gene            complement(4611..5072)
                     /locus_tag="PLF29_01260"
     CDS             complement(4611..5072)
                     /locus_tag="PLF29_01260"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HXK52601.1"
                     /translation="MTSKKSTTKVVEELQKKIEEYEENWKRALADYKNLQKRVEEERA
                     EIFGYANKNLIQRFIPVLDNLETMAKHFEDQGIKLILKQLIQILEEEGVAEIESDKKT
                     FDPHEMEAVEHVETGGDLDNKVIETTQKGYKMGNKVIRPARVKVGKTKGEK"
     gene            complement(5078..5671)
                     /locus_tag="PLF29_01265"
     CDS             complement(5078..5671)
                     /locus_tag="PLF29_01265"
                     /inference="COORDINATES: protein motif:HMM:NF013768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HXK52602.1"
                     /translation="MARLLSEGFLQMEHTSSGRIPTSSAYKFFLEELMEEEELDVLQE
                     VAMKQKLWANRYEFEKLLRQAVLALSEITQELALATTDDGYVIHAGSVNLLDHKEFTD
                     ENTIKSILHMLDRHEALAKIFEKISLVDDGEVKCLIGADLEHPNLEGCSAIVAKYSVG
                     KRKGYIAVLGPSRLQYQRIIPSLRYTKNLVEELGGSW"
     gene            5942..6856
                     /locus_tag="PLF29_01270"
     CDS             5942..6856
                     /locus_tag="PLF29_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52603.1"
                     /translation="MTARLIRNYLIKFFFTREYNLYILLRLICTLGPLLVTFLFSLGI
                     YRIEQRMSLSEIVLPLALILVTEIIEHLVRVGSKSLISFTTEKKLVEVQTDFLITFSP
                     KEKDRMEVVQAVRNLTHAIRRFVTHMIDDGFQGIVEFILIPFILLLIDVRVFYLEVSA
                     ISFYIISSYFLSRHYEVLYEKFDQVREKYYSRLLVNDRVKSYAEKVWGKYGDVQSFRF
                     ISWFSLQSLVSFFKLSILLLVVRDVFMGTKQISELVLIAGYVVSTKKFLNDVTNIFEY
                     YMEIKAGTRRLEDVIKLKKLKSYISSIT"
     gene            complement(6845..8656)
                     /gene="lepA"
                     /locus_tag="PLF29_01275"
     CDS             complement(6845..8656)
                     /gene="lepA"
                     /locus_tag="PLF29_01275"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015134967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HXK52604.1"
                     /translation="MNHTANIRNFSIIAHIDHGKSTLADRLLDITGTIEKRQLKDQTL
                     DSMELEREKGITIKLKAVRMNYTLDSIEYQLNLIDTPGHVDFSYEVSRSLAACEGAVL
                     VVDATQGIQAQTVSNVYKAIDANLTIIPVINKVDLAGSNVEKTAQDLVDTFGFKHDEI
                     LQISAKTGLGIERLLSEIIKRVPAPKDTYDKNFRGLIFDTYYDEFLGVIALIKVEDGK
                     IELQDVKNHRKIYLMATKAESDPESIGIMVPHRKEVGSLSAGEVGFISTGVKDIKTVR
                     VGDTVTFKDIASATQQLEGYKEVKPFVFMSIYPIENDKYPELKDALEKLSLSDSALQY
                     TAESNSALGFGYRCGFLGLLHADIVQERLEREYNLELISTTPSVEYTATLTTGETVKV
                     KNPSEMPEPTRIQSVQEPWIILHIFSPATYTGGIITLCESRRGIMRKMEYPTQDTIMF
                     EYELPLSELVNNFFDELKTISSGYASIDYDFLEYRNVKVIKMDILVHGAEVEPLAQIV
                     LKDNAQAIGKKIINRLKDVIPRQQFAVAVQAAIGGKIIARETIPAMRKDVLSGIYGGH
                     RERKDKLLERQKKGKKRMKRLGKVDIPQEAFRQVLSN"
     gene            complement(8716..10377)
                     /gene="murJ"
                     /locus_tag="PLF29_01280"
     CDS             complement(8716..10377)
                     /gene="murJ"
                     /locus_tag="PLF29_01280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HXK52605.1"
                     /translation="MKKFYKNITNKIKIRGRWSFIAKAQKTILSAAFVIALFAGLSSI
                     LGFVKGRMLINFFGVSDELGIFYAADRVPNMVYSIIVAGAISTIFIPVFTDLLKKDKK
                     EAWKTASNVINITLISFLFLGGFLVIFAYPVMNLLSLGELTPEQLKLGAGLMRIMVGS
                     QLILILSSLLSSILQSHKFFLVPAAAPVLYNVGMIVGLIALSGKYGIYAPALGTVVGA
                     ALHLLIQIPLLKKTSFSYVVSKTLLNKETRKIFSLVPPRIASVVLTQLSIVVNNSLAI
                     LISPASVVILKSASQVQFLPVNIFGASMALASLPVLAEHADTSEHDKFKRIFITTLHQ
                     TAYLVLPFSALMLVLRVPIVRIVYGVANFPWEATIETALTLGFFSLSIFSQSVVYLFT
                     RAFYALKDTTTPVTVSLLTIIINTTLSVFFVHFLRLGVWSLAMSYTITSLLDSILLFY
                     KLDRKLNGFILSEVLVPLIKISTASAMMGVFVYVPMKILDLYVLDTSRTIQLIITTGT
                     SAASGLGIYLLLTKLLKVEEVNLFYKLILKIKPKKTFQEVVSEEQ"
     gene            10659..11036
                     /gene="rpsT"
                     /locus_tag="PLF29_01285"
     CDS             10659..11036
                     /gene="rpsT"
                     /locus_tag="PLF29_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HXK52606.1"
                     /translation="MANTQSAKKAVRSSAKKRLHNIFWKTKIKASVKAIEKLLSTNTK
                     ELDIIKEQERVLYKVVDKAAKNNVIHKNKAARIKSKISKKVSANAQGPEKEKNTAEKE
                     TKVSTASKEKASKSKAKTKPATK"
     gene            11158..11562
                     /locus_tag="PLF29_01290"
     CDS             11158..11562
                     /locus_tag="PLF29_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52607.1"
                     /translation="MIILLLNFSVNRQLEQLVIEVEDLSDTFSSRKDIIADVTKVNDR
                     IATYKSIESKRSLISRHAEVMNTDVPKGFYSSTSFDTDKANLNIVVSEPINASIYMSN
                     LFSNKDIESISLLYAGLNRGGSYDISIEVTYK"
     gene            11559..12140
                     /locus_tag="PLF29_01295"
     CDS             11559..12140
                     /locus_tag="PLF29_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52608.1"
                     /translation="MKLNNYYDREIQSRPYRNFVLFLCFLAFNVVVVGYFGAVRGYSA
                     YKYKKDLVKEFSEQGTIIKNNINRVALLEPYLVRNLKTEIIMNGVPVEKDYDGMIFEL
                     SQIARINGLSLDEVKGTSSNDSSVTKSDYVEYSELYSAKLTGDISALPKFLQDLSVSK
                     RVYAVVSARADVDSNPNSGKDTLIVTFAIYYIN"
     gene            12198..12386
                     /locus_tag="PLF29_01300"
     CDS             12198..12386
                     /locus_tag="PLF29_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52609.1"
                     /translation="MKWFLLIFCSSIFILFIVLSKGREEISRSFEISEQARINAVPFK
                     GDLDIQYIDSIRIPAYEY"
     gene            12376..12876
                     /locus_tag="PLF29_01305"
     CDS             12376..12876
                     /locus_tag="PLF29_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52610.1"
                     /translation="MNTNSQAKNGLKTFIITFSISVLVFSALYFMVSETSVDSVSISD
                     SSISSKNAQKKTPGDNEAVMGIYSIKETGSAEPTTTSVTQATDGAMEEPSVFASLVTG
                     AGDSESVGGVNTPAVLAAAAGGGGGVIQTTQTTAPVPVTGSTSVTLGLLISLTLFTKL
                     ANPKLR"
BASE COUNT         3380 a         2605 c         2422 g         4493 t
ORIGIN      
        1 tctcacaccc ttttggccca cacagctgcc caccggatct atttttgcgt cgtttgatga
       61 aaccgctatt tttgttcgtg agccggcttc tctagctata gcttttattt ctacgacccc
      121 ggaggacact tcaggaactt cctgttcaaa aagccttctt actaattcag cgtcgctcct
      181 gcttaaaatt acttctgtgc ctctagggcc tgttctaacg tcttttacgt aaacttttag
      241 tctttgatta attctgtaat gctcattctc aacttgttcc gactgaggta gtacgccctg
      301 tgcttttccg atgtccaata taataagacc gttctcaatt ctaaaaattt gtcctgtaat
      361 taagattcct atcttatctt taaattcgtc gaataagacc tttttctcgc tttcgcgtat
      421 tttttgcaaa ataacctgtt ttgccgtctg agctgcaatg cggccaaatc ccggaggcgt
      481 aacgtctttc ttgtctttaa ttattttagc ttctccggtg tcaggatcaa caattacagt
      541 aatttcctct atttgctctt cagtgagatc agccccataa tcttttttgt atgccgaaat
      601 cagcgcactt ttaatagact ctataacatc ttcggccgat attcctcttt cagttgccac
      661 ctgtattaat gctgctttaa attctgttgt tgccattttt ttatccaaat ttattacttt
      721 agcaattaac ctttactaac agtataattc tatcaaaaaa ggcgggaact tcccacctca
      781 ttttgttttt atagttaatc tttccaagcc taagcttggg ctaagaataa tggttttttg
      841 ctaataggtg tttttaagca ccttttaagt ccactaggta aattaccaca cattaagtgt
      901 gttggtcaag cttggcctca ggatgacgat tcgggcatgc ctttaactag tttcgcggcg
      961 ctgcccgtta agtgcgagct gtcggcccac ttgactaagg agtaagatag gttttgattg
     1021 ttttgtgtga ccgacagctc gcttattgag cagttatggt tttgtatagc agaaaactct
     1081 tttttatttt ctattttaca aagatggatc actccggctg caaagatgcc tgcatgccca
     1141 ataactgcaa tattaccgtg cagagttttt ttgattgctt ctagtaggcc cgttgcaaag
     1201 cggttaacta gctgagagta gttttctcct tcaggaaagc ttatgtcttc ttcgcccata
     1261 taccatttat gtgtgattaa aaaatattgt ttccaacttt cttcatttgg cttcatccct
     1321 tcgacctttc ccatatttat ttctctgaag ttttctaaaa tttttggtat tagtttcttt
     1381 cttttagcta tatattctgc cgtttgcatc gctcttttaa gaggagaaga gtagattgca
     1441 tctatggtta tattatcaaa ggctgtagca gtttgagctg cttgaaaatg tcctttgttc
     1501 gtcagctcat agtctatgtg tttataagaa agttctctgg taatgtttgc taggttttct
     1561 ccgtgtctaa ctagataaat agttgtcatg attctttaac ttcccatgtt aagtttgtgt
     1621 gtatgaaagt gtacaggaaa atctagcaac ttaaaatagt gcattgatga ttatgttatt
     1681 ttaagaccgg cgttgtacta aagtcgcccc cttaccatgc ctgttttatg agtttttacg
     1741 gtattcttca agtagctgtt tctgtttctt tgaaactttt tttggtgtta ttacctcaac
     1801 ttgaactatt aaatctcctt gtcctgttcc ttgaagtttc ggcatacctc ggtttttaag
     1861 cctgaattgg gccccgtttt gagtacccgc tggtatttta agtttcacgt ggcccatgcc
     1921 tgttttctct gatgtatcta ttacatctac aggcacctcg tcacccaata tagcctgaat
     1981 gaagtcaatt tttagtagtg tcccaagatt atctccggct ctttgaaaaa tcttcggtgt
     2041 tgcgatcctt aatgttataa ataaatctcc gttaggtatg tcttttgccg gacccggcat
     2101 tcccttgccc gcgaatctta gctctatacc gtctctggct ccttgcggga tttttattgt
     2161 tattttgccg gcttctgtgg ttatatcttt ttttgcacca tgcactgcgt cttcaaatgc
     2221 aacatgtagt tcgtagtaaa gattctttcc tttgttttgc cttcttcccc cgaagccgcg
     2281 aaacccgaaa aattgttcga atatatcaaa aggatctatt ccgtcaaagc ccgcgcctcc
     2341 cgttgtgtag gtatagctga aaggacctcc ggcgcctccc ccgctaaaac cttgtccggt
     2401 atttcgatat ttagcgcctt cgtggccgaa ttgatcgtac atttttcttt tatcgggctc
     2461 gcttaatatt tgataagctt catttatttc tttaaatctt ttctcagcag ctgccttgtc
     2521 cccatctttt accatatccg gatggtgctc tctggcaagt tttctgtaag cgctttttat
     2581 ttcttccgga gaggcgcttt tagaaatgcc tagtatttca tagtaatctc ttttttccat
     2641 attgttcttt tctttctctt agttttaacg tagtgtgcgg gccttacggc ccgcacacca
     2701 aaagacactc agaatctgtc cgtaaactct actcttgcac cacttcccct tctgttactt
     2761 catcctccgg accttctctt ttagggtcat ttccttgctc ttccgtgccg gtttgtgatt
     2821 gctttgcaag gtgctctcct attttctgca tagcgctact taactcttcg gttttttgct
     2881 tgagttcttc tgtggaagct gtggacagaa catctctgac acccttcacc ttagctgtaa
     2941 tatcagaaac aatttcctct gtcagcttac cttcggaatc ctttaaggct ttctctgcgg
     3001 catggcacat gttatcagct atattcctgg cctctatttg ttcttttttc tttttatctt
     3061 cttcagcatg aagttgggcg tcttttacca ttttatctat ttcttcttga ctaaggcctg
     3121 ttccaccttc aatctttatt ttttgttctt tgcctgaggt cttgtctttg gcgctaacgt
     3181 gtattatgcc gctggcatct aagtcaaatg taacttctat ctgaggaacg cctcttgggg
     3241 ccggcggtat tccgtctaat ataaagcggc caagactctt gttatctatt gccatttcgc
     3301 gttctccttg aagaatgttt atttccacgc tagtttggct atcggcagca gtgctgaata
     3361 tttgatttgc tgcagtcggc actgttgtgt tccgtttaat taaaggagtc ataattccgc
     3421 ctagtgtttc gatccccaag gttaggggtg tgacatcgag tagtagaaca tcttttacgt
     3481 ccccacctag tacgccccct tgtatagcgg ccccaattgc aacaacttca tcggggttaa
     3541 ctcctttatg cggctctttt ccgaaagatt ttttaacaac ctcgttcact tttggagttc
     3601 tagtcatacc tccgaccatc acaatttcgt ctatctgccc tttatctaag gacgcatctt
     3661 ttagtgcgtt ttctattggc ttgagtgttt tattaatcaa ctcctcgact agttgttcgt
     3721 atttagatcg agtgagttta atattcatgt gttttgggcc atttgcatct gcagtgatga
     3781 aaggtatatt tacttctgtt tcaagagtac ttgatagctc tattttggct ttttccgctg
     3841 catcttttag tctttgcatt gcttgcttgt cttgagttag ttctatgcct gtttctttct
     3901 taaactcctc aagcagccaa tttattaaac atacgtcaaa gtcatctccc cctaaatgtg
     3961 tatctccgtt cgttgattta acctcaaaaa ctccgtctcc cacctcaagt acagaaacat
     4021 caaaggttcc tccacctaag tcataaacca ctatcttttc atttgatttc ttatccaagc
     4081 cataggctag agaagctgca gtaggctcgt taattattct tttaacctct aagcctgcta
     4141 tttgtccggc ttctttagtt gctgatcttt gcgcgtcatt aaagtaggca gggacggtaa
     4201 ttaccgcctc tgttacttct tgccccaaat atttttcagc atcagcttta agttttctta
     4261 aaatcatggc tgatatttct tgtggcgtga aggttttccc gtctacctct actgcagcca
     4321 ttccattttt accttccact atagtgtagg gatagttttt tttgagtgtg tcaatctcag
     4381 gatcgttgaa cttcatacct atcagccttt tgatgctgaa aatggtgttt tttggattta
     4441 ccacctgctg tctctttgca gctacgccta ccaagtttgc agtagggttt actatcgagg
     4501 gagttgtatt cataccctca gagtttggaa ttatttttgg ttggccactt tccattacgg
     4561 ccatcgcact gtttgttgta cctaggtcta tgcctattat tttacccata ctatttttct
     4621 ccttttgttt tgcctacttt tactctagca ggcctgataa ctttgtttcc catcttgtat
     4681 cctttttgag tagtctcaat aactttgttg tctagatcgc ctccggtttc tacatgctca
     4741 actgcttcca tttcatgtgg atcaaacgtt ttcttatcgg actctatctc tgctacaccc
     4801 tcttcttcaa gaatttgtat caattgtttt aaaattaatt ttattccttg gtcttcaaag
     4861 tgctttgcca tagtttccag gttgtccaga acaggtataa atctttgaat taggttttta
     4921 tttgcgtacc cgaatatctc agccctttct tcctcaaccc ttttttgaag atttttatag
     4981 tccgctagag ctcgtttcca attttcttcg tattcttcaa tttttttctg tagttcttcg
     5041 actacttttg ttgtgctttt tttacttgtc attttgatta ccaagaaccg ccaagttctt
     5101 ctactaaatt tttagtgtac ctaagtgaag ggattattct ttgatactga agtcttgagg
     5161 gacctaatac cgctatataa ccttttcttt tccccacgct gtattttgcg acaattgcgc
     5221 tgcaaccttc taagttcggg tgctccaaat ctgcgcctat cagacatttt acttctccgt
     5281 catctactaa gcttattttt tcaaatattt ttgctagcgc ctcgtgtctg tccagcatat
     5341 gtagtatgct ttttattgta ttttcatcag tgaactcctt gtgatccaaa aggttaaccg
     5401 atcctgcatg aattacgtat ccgtcatcgg ttgtagctag ggccaattct tgcgttattt
     5461 cagataacgc gagtaccgct tgtcttagaa gtttttcaaa ttcatatctg ttagcccaaa
     5521 gtttctgttt catcgcaact tcctgcagga catccagctc ttcttcttcc atcaattcct
     5581 ccaaaaagaa cttataagcc gaagaagttg gaattcttcc cgaagatgta tgctccatct
     5641 gcaaaaaacc ttcgctgagc aatcttgcca tttcgtttct aactgtggca ggggagcatt
     5701 taacgttgta tttctcgaca ataagagagg acccaacagg ttgcgcagta tctcaatact
     5761 cttctatgat agcttttagt aggtttatct gtctttctgt taacaacata ttgtcactat
     5821 attatcatga gtgctaagag tgtcaagcag tatggtttgc ttgtattaat ctttgaaatt
     5881 cttacaaaac ctataatgca aaatttgttt aaactagtgt ctagctgtgt ataattgatt
     5941 catgactgcc cgtcttataa gaaactactt aattaaattt tttttcacgc gagagtataa
     6001 cctgtatatt ttgttgcgtc ttatttgcac cttagggcct cttttagtaa cttttctatt
     6061 ttcattaggc atttatcgta tagagcaaag aatgtccctt agcgaaatcg ttttgcccct
     6121 tgcccttatt ctagttacag aaattattga gcaccttgtt cgtgtcggat caaaatcttt
     6181 gatttctttt acgacagaaa aaaagcttgt tgaagttcaa acagactttt taataacgtt
     6241 ttctccgaaa gaaaaagacc gtatggaggt agtgcaggca gttcgtaatt taactcatgc
     6301 cattagacgg tttgtaactc atatgattga cgatggtttt cagggaattg ttgagtttat
     6361 tttaatccct ttcatcttat tgttgattga tgtacgagtc ttttatttag aggtttctgc
     6421 gatatctttc tatattatct cttcttattt tctttcaaga cactatgagg ttttatacga
     6481 aaaattcgat caggttagag agaaatatta ctcacggctt ttggttaatg atcgcgtaaa
     6541 aagttatgct gagaaagttt ggggtaagta tggtgatgtt cagtcttttc gttttatttc
     6601 ctggttcagc cttcaatcat tagtgtcttt ttttaaactt agtattttgc tacttgttgt
     6661 gcgcgatgta tttatgggta ctaaacaaat ctccgagctc gttctaatag ccgggtatgt
     6721 tgtttcaaca aagaagtttc tcaacgatgt gactaatatt tttgaatatt atatggagat
     6781 aaaggccggc accagaaggc tagaagatgt aattaagctg aaaaaattga agtcctatat
     6841 ctcgtcaatt acttagaact tgtctaaagg cttcttgcgg aatatcaact tttcctagtc
     6901 tcttcattct ttttttacct tttttttgtc tttctaataa cttatctttt ctttctctgt
     6961 ggcctccata tatgcccgat agaacatctt ttctcatggc cggtatggtc tcgcgagcta
     7021 tgatttttcc gcctatggcg gcttgaacag cgactgcaaa ctgttgtcgt ggtattacat
     7081 cttttagtct gtttattatt tttttgccga ttgcttgggc gttatctttt agaactattt
     7141 gtgccaaagg ctcaacctca gctccgtgta ccagaatgtc catttttatt acttttacat
     7201 ttctgtattc cagaaagtcg taatcaattg atgcatatcc ggaagatatt gttttgagtt
     7261 catcgaaaaa attatttaca agttcagata gaggcagttc gtattcaaac ataatcgtgt
     7321 cttgtgtagg gtattccatt tttctcataa tgcccctccg actttcacat agggtaatta
     7381 tccctcctgt gtatgttgcc ggcgaaaaaa tatgtaaaat aatccacggc tcttgcacac
     7441 tctgtattct agtgggctcg ggcatttctg atgggttttt cacttttacg gtttcccctg
     7501 tggttagtgt tgcggtatat tctacgctag gggtagtcga tatcagttcc agattgtatt
     7561 ctctttcaag cctttcctgc actatgtcag cgtgaagaag tcctaaaaaa ccgcaacgat
     7621 atccgaatcc tagagcggag ttactttcgg cggtgtattg aagggcagaa tctgataaag
     7681 acagcttctc taaagcgtct tttaattcag ggtatttatc gttttcaatt gggtagatgg
     7741 acatgaatac gaagggttta acttctttgt atccctccag ttgctgtgtt gcggaggcga
     7801 tatctttaaa agtaaccgtg tcgcctactc ggaccgtttt tatatctttg acgcctgtcg
     7861 agatgaagcc aacctccccc gcagataaag atcccacttc tttgcggtgg ggaaccatta
     7921 ttcctatact ttcaggatca gactctgctt tggttgccat taagtatatt tttctatggt
     7981 ttttcacatc ctggagttca atttttccgt cctcgacctt aataagcgcg atgacgccca
     8041 agaactcatc gtaatatgta tcaaatatta atcctcgaaa attcttgtca taggtatctt
     8101 ttggtgcagg aactctctta ataatttcgg aaagtagccg ttcaatcccg aggccggttt
     8161 ttgccgagat ttgcaaaatt tcatcgtgtt taaaaccaaa agtatccact aaatcttggg
     8221 ccgttttttc cacattactt cctgccaaat ctaccttatt tatgaccgga atgattgtta
     8281 ggttagcatc tatagcttta taaacatttg aaacggtttg tgcctgaatt ccttgtgtag
     8341 catctacaac taaaacagcg ccctcacaag cagccagaga gcgtgaaact tcataggaaa
     8401 agtccacatg tccgggcgta tcaatgagat taagctgata ttcaattgaa tcgagggtat
     8461 agttcatgcg aacggccttt agctttattg ttataccctt ttctctttca agctccatgc
     8521 tgtctaacgt ttgatctttg agttgtcttt tttctatagt gccggttata tccagcaagc
     8581 gatccgccaa ggtagacttg ccgtgatcaa tgtgggcaat aatactaaaa tttctaatat
     8641 tggctgtatg gttcacaaaa gtactttata attcttgatc taaaactaca acccctctct
     8701 gtattgaaaa gataattact gttcttctga aactacttct tgaaaagttt ttttcggttt
     8761 aattttgagt atcagtttat agaatagatt cacttcctct acttttagta gtttagtaag
     8821 aagtaggtag atccctagcc ctgacgctgc agacgtgcct gtggttatga taagttgaat
     8881 tgttctggac gtatcaagaa cgtacagatc aagtattttc attggcacgt aaacgaaaac
     8941 acccatcata gcggaggcgg tcgatatctt tattaaagga actaaaacct cggataatat
     9001 aaatccgttt aattttctat ccagtttgta gaaaagtaag atagagtcca gaaggctggt
     9061 aattgtgtaa gacattgcga gagaccacac ccccaatctt aaaaaatgta caaaaaatac
     9121 tgaaagagtt gtatttataa taatggtgag caaactcact gtgacaggag tggtagtgtc
     9181 ttttagagca taaaaagctc tggtaaacag gtacactacg ctttgagaaa atattgataa
     9241 gctgaaaaat ccaagcgtta aggctgtctc tatcgtggcc tcccacggaa agttggcaac
     9301 cccataaaca atcctcacta tgggaactct tagaacaagc atcagggcgc taaaaggaag
     9361 tactaggtat gcggtttggt gtaaggtggt tataaaaatt cgtttaaact tgtcgtgctc
     9421 gcttgtatcc gcatgttccg caagaacggg gagcgaggct aaagccatag aagccccaaa
     9481 aatgtttaca ggtaaaaact gaacttggct tgcggatttt aagataacta ccgaggcagg
     9541 agatataagt atggcaagag agttgttgac cacaatggag agctgtgtta gaaccacact
     9601 cgcaattcta ggcggtacaa gtgaaaaaat ttttcgtgtc tctttgttta gcaatgtttt
     9661 acttacaaca taggagaagc ttgtcttttt taataaaggt atctgaatta gcaaatggag
     9721 tgcggctcca actactgtgc ccagcgccgg agcatatatc ccatattttc cggatagagc
     9781 aatcaagccg acgatcatgc ccacattgta aagtactgga gctgcagctg ggactaaaaa
     9841 gaacttatgt gattggagaa tacttgagag caggctgctc aaaattaaaa ttaactgtga
     9901 tcctaccatt attctcatta agcccgcccc tagcttaagt tgttccggcg ttaattcgcc
     9961 cagggataaa agattcatga caggataagc aaaaattacc agaaatcccc ctaaaaaaag
    10021 gaaagaaatc agtgtaatgt taatgacgtt cgaggctgtt ttccacgcct cctttttgtc
    10081 tttttttaat aaatcggtga atacaggaat gaatatagtg gatatagctc cggcaactat
    10141 tattgagtac accatgttgg gcactctgtc tgcggcgtaa aagataccca gctcgtcgct
    10201 aactccgaaa aagttaatta gcattctccc tttgacaaag cccagtatgg aagaaaggcc
    10261 tgcaaagagt gcgatcacga atgctgccga aagtatagtt ttttgcgcct tggctataaa
    10321 gctccatctt ccgcgaattt ttattttatt ggttatgttt ttgtaaaact ttttcatttt
    10381 tttgtatcta tgtatgtttc tttttagcaa aaattacggc ctgtgtacac agccgattat
    10441 ttaagcaccg tcttaatttt tgctatctta tataatttcg ttacttaaaa ctacttgcgt
    10501 aagctagtaa atgtagtttg cccaacatgt tgggctttac agcgcaaaga aattacagca
    10561 aatcacctgc gtcagctggt ggtggttgat cagttatggg gtcctagtat aacaaagtat
    10621 tgtcaaattg tgctactgtg ttatcatact accgcagtat ggcaaataca cagtcagcaa
    10681 aaaaagccgt cagatcttct gcgaagaaaa gacttcataa catattctgg aaaaccaaga
    10741 ttaaggcttc tgtgaaggca atagaaaaat tgctttctac aaatacaaaa gaacttgata
    10801 taatcaaaga gcaggaaaga gtattgtata aagtagtaga taaagctgca aaaaataacg
    10861 ttattcataa gaacaaggct gccagaataa aatcaaagat ttcaaagaaa gtatctgcca
    10921 atgcccaagg tccagaaaaa gaaaaaaata cagctgaaaa agaaactaaa gtttctactg
    10981 ccagcaaaga aaaagcttca aaatctaaag caaagactaa accagccact aaatgagccg
    11041 ctagaggcaa ctgagcctat ttatacccca aacttatttc aagaaaaaag tattagtatt
    11101 cccgactcta ccccgaggat aatagtttat ttaatcattt ttattcaggt aatatatttg
    11161 attatccttc tgcttaactt ttcagttaac cgacagcttg agcagcttgt aatagaagtc
    11221 gaagacttgt cagatacttt cagcagcaga aaagatatta ttgccgatgt tactaaagta
    11281 aatgatcgta tagctactta caaatctata gagtcaaaaa gaagtctgat cagcagacac
    11341 gcagaagtaa tgaatacgga tgtgccgaag ggtttttact ctagcacgtc atttgatact
    11401 gataaagcaa acctaaacat tgttgtttcc gagccgataa acgcttctat ctatatgtct
    11461 aatctttttt caaacaaaga tatagaatca atcagtttat tgtacgcagg cttaaatcgc
    11521 gggggctcgt atgatatttc gatagaggtt acatacaaat gaagttgaat aattattatg
    11581 atcgagaaat acaaagccga ccttacagaa attttgtttt gtttctgtgc tttttagctt
    11641 ttaatgtagt tgttgtggga tatttcggtg ccgttagggg ttattccgcc tataaatata
    11701 agaaagatct cgttaaagag ttttcggagc aaggaactat tataaaaaac aatataaatc
    11761 gtgtagctct gctcgagcct tatttagtaa gaaatttaaa aactgaaatt attatgaatg
    11821 gagttcccgt tgaaaaggac tacgatggta tgatttttga actatcacag atagcaagga
    11881 ttaacggact gtcgcttgat gaagtcaagg ggacatcctc caatgattcc agtgtgacga
    11941 agtcggatta tgttgaatac tctgagctct attctgctaa gttaacagga gatatttctg
    12001 ctttgcctaa atttctgcag gacctgagtg tctcaaaaag agtatacgca gtggttagtg
    12061 cacgggcgga tgtagattcc aatcccaatt ccggaaagga tactcttata gttacatttg
    12121 ctatttacta tattaattga tactaagctt tgcattttta tgaagtattt gtttgtagaa
    12181 agatataaaa taataatatg aaatggttct tgttgatttt ttgctcaagt atctttatac
    12241 tttttatagt actttcaaaa ggaagagaag agatctcaag atcctttgaa atttcggaac
    12301 aagctagaat aaatgctgtg ccgtttaagg gagatctcga cattcagtat attgatagta
    12361 ttaggattcc tgcctatgaa tactaacagt caagctaaga atggcttaaa aacatttata
    12421 ataacttttt cgatatcagt tttggttttc agtgcgctgt attttatggt ttctgagact
    12481 tccgttgatt cggtttcaat ttccgattct tcaatttcct ctaaaaatgc tcagaaaaag
    12541 actccaggcg acaatgaggc cgttatggga atttattcaa taaaagaaac aggttctgct
    12601 gaacctacaa caacttccgt gactcaggca accgatgggg caatggaaga gccaagtgtt
    12661 tttgcatctt tagtaaccgg tgctggtgat tccgagtcgg taggaggagt aaatacacct
    12721 gcagtcttag ctgctgcagc aggaggtggc gggggagtaa ttcaaacaac tcaaactacc
    12781 gcaccagttc ctgtaactgg ctctactagc gtaactttgg gtttgctaat ttcgttaaca
    12841 ttgtttacga aattagcaaa cccaaagtta cgctagtaga gccagttaca ggaactggtg
//
LOCUS       DAOSWN010000014        12584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_3182_length_12584_cov_4.759678, whole genome shotgun sequence.
ACCESSION   DAOSWN010000014 DAOSWN010000000
VERSION     DAOSWN010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12584
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3182_length_12584_cov_4.759678"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PLF29_01310"
     CDS             <1..409
                     /locus_tag="PLF29_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52611.1"
                     /translation="SPAFCYCPSAGAAIIGDGEGNVTLYEYYRAKRILTPTTKFQAVS
                     QGNLHLSSGNFYHITAANGEHMQHSAKSFFNVLNEPRNSALLGLTTATVYREGLPQQV
                     TAFRLIKKLWVPSDERLGFKFGRPITRIVFIPE"
     gene            530..1288
                     /locus_tag="PLF29_01315"
     CDS             530..1288
                     /locus_tag="PLF29_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52612.1"
                     /translation="MLNTETGVTRLKTKWDPDTRWDFVTAVMGAQAAYRLPVNGEPPM
                     IELERARSVLAGEVKTILASVPKEIAIRAMSEMVKAKQEEVRAGMGFTTSGRVIEAYD
                     NRGPGFPVKRRYLEITPQGLVVFYGKNGHITVVHQKTPEQEGWGISNFVIAPGAANVD
                     VYPAWAFTEMKQYFAGATTRVRAQKNTPRNLSPAEKRAQLRGVLGGYYSVRCVTDGSN
                     PTLTFFQLPNPDSPLNTEVGSPKIEPVLIITIRG"
     gene            1370..2326
                     /locus_tag="PLF29_01320"
     CDS             1370..2326
                     /locus_tag="PLF29_01320"
                     /inference="COORDINATES: protein motif:HMM:NF017508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HXK52613.1"
                     /translation="MKTKITKEQKSTVKLQVTVPADKVKSTYNSIFEELVKNTELPGF
                     RKGQAPKNLVEEKTDVSKLYGDVINELLKIYYPQALKEHLLQPIANPKVEVTEFDLDK
                     DFEFTATVAIMPDVKVGDYIKALKKIHKENSEKIRAENAEKLKRGEAITGHDHAHLSP
                     DEVVNSIVEVTTAEVADLVIEDEVDRMMTRLVDQAQAVGISLEQYLKSQNKTADQLRE
                     DYGVIALRNLKAEFALDHLLKKSKIEVSDAEVEEMVKATGDTETEALMQNPMQKMYVK
                     SILAKNKLISNIIKEVEGDLHSEDNSQTKKNSGPSKAGKGKK"
     gene            2326..2928
                     /locus_tag="PLF29_01325"
     CDS             2326..2928
                     /locus_tag="PLF29_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HXK52614.1"
                     /translation="MSTLIPIVIEKDPKGFERSYDIYSRLLKDFVVFVTGAIDMALAN
                     TFIAQMLFLESENPDKTISVYINSPGGSVYAGLAMYDTIKHVKNEVSTINVGLAASAA
                     SLLLASGSKGKRYALPNTYSMIHQPLIGGGGIEGQATEIEIEAKHMITLKHQFADLLA
                     KDTKKTKETVLLDFERNRYMDAKETKAYNLIDEIMAPNKK"
     gene            3023..3099
                     /locus_tag="PLF29_01330"
     tRNA            3023..3099
                     /locus_tag="PLF29_01330"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3057..3059,aa:Val,seq:gac)
     gene            3162..4508
                     /locus_tag="PLF29_01335"
     CDS             3162..4508
                     /locus_tag="PLF29_01335"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HXK52615.1"
                     /translation="MLGLAPQRNYSKTPAYAGDDRGKKILEGIKLKKNYKRLFFPNNF
                     FWGAATSAHQVEGYGVNNWSEWEKSKKRLDELKRSGELAIYGQANYISGKACNHYDLY
                     EEDFQLAKDLGHNCTRLSISWARIQPDKDRFDKSAIEHYKKVLLFLRKLDLEPFVTIW
                     HWDLPLWLTEEGGWLSSKTPEYFRSYVKILCDELGDLVNYWITINEPLVYASNSYQKG
                     SWPPQHKSFAKVYKVLKHMIKGHILAYKDIKQNNEKAFVGIAKNNVYFGGGLFKKPLR
                     YFWNYYFLNKLEQRYDFIGLNFYFENNIHSLLEKLLRKSHRKKKLKYSDMGWLLKPRS
                     IKYTIAELKKYGVPIFITENGLADAEDKYRKDFISQALYGVYEAIQQGADVRGYMHWS
                     LMDNFEWAEGFWPRFGLIGIDYKTQKRKIRPSAHYYKKICTSNSLDEKEIIKSNIF"
     gene            complement(4741..5313)
                     /locus_tag="PLF29_01340"
     CDS             complement(4741..5313)
                     /locus_tag="PLF29_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52616.1"
                     /translation="MDTNEKSYLYLTAVVVVFLALLGLWVVSNKNGIEDTKIVEKSSS
                     KSTITVSTDGETVTSEIDSSSENTDLNTELKNKAELSYIKTSLVSVTKDSSLKGEATI
                     SRTDLGLDHEVKAWLPDPQEGYSYEGWLVKDAAKGEFFSTGVMSKNDEGVYVLEYSSE
                     DPSLGYNTVVITLESVVDETPEEHVLESQM"
     gene            5398..6600
                     /locus_tag="PLF29_01345"
     CDS             5398..6600
                     /locus_tag="PLF29_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXK52617.1"
                     /translation="MRNIAPTKDQIEVLNKLIKWYKNETRNYITLGGYAGTGKTTVVS
                     LFRVLLGQHEKKLKVAFCSYTGKGALVLKNRLASLQARFPKDTVGTIHSLIYTPLVAS
                     NNEIIGWELKDDIKYDLLVVDEASMLDSKIWSDLLSFGIRIIAVGDHGQLPPINGIFN
                     LMQDPELKLETIHRQASGDPIIGLSVLARKKGHIPAKSFGTKTKKLDSSLPESHEIIM
                     SLLQNWSEDTIVLCGYNSTRVKINNFVRTALGIESPEPTVGDRVICLRNNHQKNIFNG
                     MIGTITYIAEENETMYYAEIIFNELPEQYSGHILKSQFNNEESINYTKNRKATLKCDL
                     FDFGYAMTVHKAQGSEAERVILFEERFPKLDDLGWRRWLYTAITRAKEELYILGPAQE
                     LKNNNNEN"
     gene            6644..7609
                     /locus_tag="PLF29_01350"
     CDS             6644..7609
                     /locus_tag="PLF29_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52618.1"
                     /translation="MSEQKSKFVIWSAVIFVMLFILSFLISIQFESIVSSPSRGFLGL
                     NLNFFLNLVVLLTLLSFVYTFSIGTWESKWHLAYIPLSIALGMFAIVAPYNLLAGIIV
                     LFTACVLIFSDTYNSTKMKENLITFDPILIMKTSIRNILLIFSLLAAVIAMINTKGRS
                     ENAFANQITTMAETQIDSILSNKDFQTYINNLTLGMGNSLNLGPVEKNIIEKNMPLTD
                     LQDKVNQNSSDPNQNTAQEEQLAFPKEEIKAVALATLNKYIKPYSQLLFIAFSAVLFG
                     FVQTSGTAAYIIYAMLARPFIKLLLALGIFKEQTKTVEQKTIILP"
     gene            complement(7596..8387)
                     /gene="truB"
                     /locus_tag="PLF29_01355"
     CDS             complement(7596..8387)
                     /gene="truB"
                     /locus_tag="PLF29_01355"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HXK52619.1"
                     /translation="MILNVYKPKNWTSFDVVAYIKKIINSASSTKKNKIKVGHAGTLD
                     PLAEGVLVVLTGADTKKQDSLMLTTKEYVCEIGFGIDSPTYDLEGPLDFNDVFLSSFE
                     NKDPVVTTQLLGSFKSKVLELMQSFTGEIEQTAPPYSAKKVGGKRLYDLARKNLLDSK
                     LLPVKTVTIYSITPVTFLHAELKNFILESGQEVLGNVGFANRKIPVFKCTISCSSGTY
                     IRSLAHDLGEQLGCSGILLSLVRTKVGEYSVENSVNVKDLSFKEE"
     gene            8561..9448
                     /locus_tag="PLF29_01360"
     CDS             8561..9448
                     /locus_tag="PLF29_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52620.1"
                     /translation="MGNGGEDGNFFLEPGSLRRGEVEKTYTTQEVFSACTRRPRPEAV
                     SLIDDIVRAGSGADGGKSWTFLYWSEDRYGRKRSGRFYAQALGEGEKPIATLITAWEP
                     NTMLVLPRPSGPVFISGLGEVGELLAGSVYTVTPENRGLFPYILGKHQLYRYLGAIAD
                     FTGNKTPAPFLEEELLLELHQNTLEDVRRADAPCRKSKAKRSWFGLLTDQFLDGFAAN
                     PTKPECGVTTATAYAAARSLLPLDYFLGRGGDGFEFCLPRYDLETRRQFIVDAYRFNV
                     ARQQRELIQSFAYADMWRD"
     gene            9653..10219
                     /locus_tag="PLF29_01365"
     CDS             9653..10219
                     /locus_tag="PLF29_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52621.1"
                     /translation="MSKSVNNTPVLRPEQNDNPTKMASAEIAELTAPRLMSYLKKEGI
                     EHEVRSGKKKNTRYNNAWSEILLREARIKIITNVSTYNKPDETLGQGDGPGKKGLVST
                     VEIRTVGPNSTDGAVVWHNSLIIADNSHTSVTFVRNGEGEMFMRPGDGVDKVITDKRA
                     QETYLLALLKKSCILKKNKQKHRISAGK"
     gene            10322..11158
                     /locus_tag="PLF29_01370"
     CDS             10322..11158
                     /locus_tag="PLF29_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52622.1"
                     /translation="MTTISSISGISGDQVAGFERASMLDKQRPKIKVTNITGAGGGAL
                     ATVVVGHDEIGGESSVIRFSVSSTDRNTAPAEIQATLLETSRVAKNELKNIFSRHSLG
                     NSDLTNLLLGANIMEREVFVVSELVTGGQSVGTETITAYLPGGNTVRVSLHAQGISGI
                     DFLGSNCWQLAYSESPSGGLTIDRGTWQIKRDVGSGNCIIAHTTSRPEVHAGAAISTQ
                     RPVAKPRNVVEAEKIAANERASLTLSSALYLVSQAIMAAKLSADQEDEDHFSLPGRAT
                     AY"
     gene            11510..12217
                     /locus_tag="PLF29_01375"
     CDS             11510..12217
                     /locus_tag="PLF29_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52623.1"
                     /translation="MAQVTPYSETPEQEHTVAYERSRSWRPVDASRVISCRKDFNELQ
                     QDIPKFDPVAYTLDGPNGKVYIRGMHGPGERLHGHVYTVETPTRVYVLSGLGLLSYFS
                     ITGDEYQNLGASFTEFWLKETHNSTLHQAGRNGNDPINMWYWMDYFLMVNESLYKNKN
                     PTNPAVWPEAHALARQLLPRTRLLESRGTNFLLYCRIAEDQGGDPIYRVMQYDQRNKV
                     ETQVKTYTYLGGEVITR"
     gene            complement(12326..>12584)
                     /locus_tag="PLF29_01380"
     CDS             complement(12326..>12584)
                     /locus_tag="PLF29_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52624.1"
                     /translation="DNNLLHTRYQSNKGNPVEAVREFLTSFISQYPNIKLKKGCSTGY
                     GETLIEKAFNLEYSLVETMAHYKAAKYFDKETTLLLNCIDY"
BASE COUNT         4284 a         2335 c         2603 g         3362 t
ORIGIN      
        1 ctctcccgct ttttgttatt gtccgagtgc tggcgcagca ataataggag atggggaagg
       61 taatgttacc ctttatgaat attatagagc gaaaaggatt ttaactccaa caactaaatt
      121 tcaagctgtc tcgcaaggaa acttgcatct aagttcaggg aatttttacc atatcactgc
      181 ggcaaatgga gagcatatgc aacatagtgc gaaaagtttt tttaatgtat taaatgaacc
      241 ccggaattcg gcgctacttg gactcaccac tgctactgta tatcgagaag gcctacctca
      301 acaagtaacc gcctttcgat taataaaaaa gctttgggta cctagtgacg aacgcttagg
      361 attcaagttc ggcaggccta taaccagaat cgtcttcatt cccgaatgat gggtgttgaa
      421 aacaaccctc atatatcctc aaagaaatat ttgcctctga cacatcgttt gaaaagcata
      481 cgaacttaag gtaaaatcac aaaacgtaaa atatagaaag gcaagaaaaa tgctaaatac
      541 tgaaacaggt gttacaagac ttaaaactaa gtgggatcct gatactaggt gggattttgt
      601 tacagcagtc atgggcgcgc aagcagcata ccgcttgccc gtaaatggag agcccccgat
      661 gattgaatta gaaagagccc gtagtgttct agctggggaa gtaaaaacta tcttggcatc
      721 cgttccgaaa gaaatagcga tacgtgcgat gtctgagatg gtgaaagcga agcaagaaga
      781 ggtaagggct ggaatgggat ttaccacgtc tggcagagtt atagaggcat atgacaatag
      841 aggcccgggc tttcctgtta agcgccgtta ccttgaaatt acaccacaag ggctagtagt
      901 attttatgga aaaaacggac atattacagt agtacatcaa aagacgcccg aacaagaagg
      961 gtggggtata agtaactttg taatagcgcc gggggctgca aacgttgacg tttatcctgc
     1021 ctgggctttt acagagatga agcagtactt tgcgggagcc acaactagag tgcgagccca
     1081 gaaaaataca ccgcgaaatc taagtccggc agaaaaaaga gctcagctaa gaggggttct
     1141 tggaggatat tattctgtaa gatgtgtaac agatggcagt aatccgacgc ttacattttt
     1201 ccaacttcct aatccagact ctcctcttaa cacggaggta ggctcgccaa aaatagaacc
     1261 tgtacttatt ataacaatca gaggctagca gaaaactgat ctctgcaata atttcggata
     1321 ttcaatcgat tttaatcctt ctaaacctaa atatggtaaa atacccttca tgaaaactaa
     1381 aataacaaaa gagcaaaaat ctacagtaaa actacaagtc acggtgccgg ccgataaagt
     1441 aaaaagtacc tataacagca tttttgaaga acttgttaaa aatacagaac tcccgggttt
     1501 tagaaaggga caagctccaa aaaatcttgt tgaagaaaaa actgatgttt caaagctgta
     1561 cggagatgta ataaacgaat tactaaagat atattacccg caggctctta aagagcactt
     1621 gcttcagcct attgcaaacc caaaggtcga ggtcacagaa ttcgatctag ataaagattt
     1681 tgaatttact gccacagttg cgattatgcc ggacgtgaaa gtgggggatt atataaaagc
     1741 tctaaaaaaa atacacaagg aaaattccga aaaaataagg gctgagaatg ccgaaaagct
     1801 taaaagggga gaggcgatta ccgggcacga ccacgctcat ctatccccgg atgaggttgt
     1861 aaattctatt gtagaggtaa caaccgcaga agttgcggat ttagtcattg aagatgaggt
     1921 tgacaggatg atgacaaggc ttgtcgacca agcacaggct gtgggaattt cacttgaaca
     1981 atacttaaaa tcacagaata agactgcgga tcaacttaga gaggactacg gagtaattgc
     2041 cctcagaaac cttaaagctg aatttgctct tgaccacctg cttaaaaaat caaagataga
     2101 agtttctgat gcagaagttg aggagatggt aaaagcaaca ggagacaccg aaaccgaggc
     2161 cttaatgcaa aaccccatgc aaaaaatgta tgttaaatca attttggcaa aaaacaaatt
     2221 gatctcaaac attatcaaag aggtcgaggg ggatcttcat agcgaagaca actcacaaac
     2281 taaaaaaaat tcaggcccat caaaagccgg gaaggggaaa aaataatgag cactttaatt
     2341 cctatcgtaa tagaaaaaga tccaaagggt tttgagagat catacgatat ctattcacgc
     2401 ctacttaagg atttcgttgt ttttgttaca ggagcaatag atatggcact tgcgaacaca
     2461 tttatagctc aaatgttgtt tttagaaagc gaaaacccgg ataaaactat aagtgtatat
     2521 ataaactctc cggggggcag cgtgtatgca ggtctcgcaa tgtacgacac aataaagcac
     2581 gtgaaaaacg aagtctcaac tattaatgta gggcttgcag cgtcggcagc gtcactctta
     2641 cttgcttcag gtagcaaagg aaagagatat gctcttccaa atacatattc aatgattcat
     2701 caaccactaa ttggtggggg tggaattgaa gggcaggcaa cggagataga gattgaggcc
     2761 aagcacatga ttacgttaaa acatcaattt gcggatttat tagcaaaaga tacaaagaaa
     2821 acaaaagaaa cagttctttt agactttgag cgcaacagat acatggacgc gaaagaaaca
     2881 aaagcctaca atcttattga tgagattatg gcaccgaaca aaaaatagcc cgcacataag
     2941 atataagttt ttgtttttaa acatggcccg ggtacgtttt tttgtttaga aattttttga
     3001 tataattgct ttcgctaggt tagggcggtt agctcagttg gttagagcgc ttcagtgaca
     3061 ttgaagaggt cacaggttcg aatcctgtat cgcccaccaa acctttgtag tttctttact
     3121 taaaattact tgcgtaagat aatagattta gtttgcctac catgttgggc cttgcaccgc
     3181 aaagaaatta cagcaaaaca cctgcgtacg ctggtgacga tcgcggtaag aaaatattag
     3241 aaggaataaa attgaaaaaa aactacaaaa gactattttt tccaaataac tttttttggg
     3301 gcgccgctac atccgcacat caagttgagg gctatggtgt gaataattgg agcgagtggg
     3361 agaaatctaa aaaaagactt gatgaactaa agagaagcgg cgaacttgca atttatggac
     3421 aagcaaacta tatatcgggg aaggcctgta accattacga tctatacgaa gaagattttc
     3481 aactagctaa agacttaggc cataactgca cccgactgtc gatcagctgg gcaagaattc
     3541 aaccggacaa agacaggttt gataagtccg caattgaaca ttataaaaaa gttctacttt
     3601 ttttaagaaa acttgatctg gaaccctttg ttacgatctg gcactgggat ctgcccttgt
     3661 ggctaactga agagggtggg tggctcagct cgaaaacacc tgaatatttt agaagttacg
     3721 taaaaattct atgcgacgag ctgggggacc tagtcaatta ttggatcacc ataaacgaac
     3781 cgctagttta cgccagcaac tcttatcaaa aaggtagttg gccaccacaa cataaaagct
     3841 ttgcaaaagt ctacaaggtt ttaaagcaca tgataaaagg acacatttta gcctacaagg
     3901 atataaaaca gaataacgaa aaggcttttg tgggaatagc gaaaaataat gtatattttg
     3961 gcggcggttt gtttaaaaaa cctttaagat atttttggaa ttattacttc ttaaataaac
     4021 tagaacagcg ctatgatttt attgggttga atttctattt tgaaaataat atacatagtc
     4081 ttctagaaaa gttgctgagg aaaagccatc gaaagaaaaa gcttaaatat tctgacatgg
     4141 gatggctatt aaaaccgcga tctataaaat acacaatcgc tgagttaaaa aaatacggag
     4201 ttccaatatt tataaccgaa aacggcctgg ctgatgcaga agataaatac agaaaagatt
     4261 ttatctctca agcactttat ggggtatacg aggcaattca gcaaggagcg gatgttcggg
     4321 ggtatatgca ttggtcatta atggataatt ttgagtgggc cgagggcttt tggcctcgct
     4381 ttggcctaat tggaatagat tacaaaacgc agaaacgtaa aataaggccc tcggcccatt
     4441 actataaaaa aatctgcacg agcaactcac ttgatgaaaa agaaataatc aaaagcaata
     4501 tcttctaaac aatacaacta aaacatatcg tggttttata aaacgcggga accaaaaata
     4561 aaacagggta gggatgacgc aggaacaaaa ttactaagcc atttttcatg gataactaag
     4621 taactaattt ttccattccg caaactcatt tttactgcca aaagacgaag ctttcgtgca
     4681 agagctaaag tttgtaatct tacaacatta aataaattgc aataattgct tgtgtccaaa
     4741 ctacatctga ctttcaagaa cgtgctcctc gggagtttca tcaaccacag attccaaggt
     4801 tataacaact gtattatagc caagagacgg atcttcacta ctgtactcta aaacgtacac
     4861 accttcatca tttttcgaca ttactccggt agaaaaaaac tctcctttag cagcatcttt
     4921 tactagccag ccctcataag aataaccttc ctgagggtca ggtaaccatg ctttaacctc
     4981 atgatctaag ccaaggtcag ttcggcttat tgttgcttct cccttcaacg aagagtcttt
     5041 tgtaactgaa acaagactcg tcttaatata cgagagctct gctttatttt taagctcggt
     5101 atttagatct gtgttttctg aagaagaatc gatttctgag gtgactgttt caccgtcagt
     5161 actaacagta atagtagatt tacttgatga cttttctact atcttagtgt cctcgattcc
     5221 atttttattt gaaacaaccc aaagaccaag aagtgctaaa aaaacaacaa cgactgctgt
     5281 taaataaaga taagatttct catttgtatc caaaaaaatc acctccctgc ttttttacgt
     5341 gtctacaatt tctatataat catagagact tatatatatt tagtttatca tggctacatg
     5401 agaaatatcg ccccgactaa agatcagatc gaagtactta acaagctcat aaaatggtat
     5461 aaaaacgaaa cacgaaacta cataacttta ggcggatacg ccggcactgg taaaacaaca
     5521 gtagtttctc tgtttagagt tcttctagga caacatgaaa aaaagttgaa agttgctttt
     5581 tgcagctata caggaaaggg ggccctagta cttaaaaaca ggcttgcctc actacaagcg
     5641 aggtttccaa aagatacggt gggcacaata cattcactca tatatacgcc actagttgct
     5701 tctaacaatg aaataattgg ttgggaacta aaagacgata taaaatacga tcttttagtt
     5761 gttgatgaag catcaatgct agattcaaaa atttggtcgg atttgctgtc ttttggaatt
     5821 agaataattg cggtcggaga ccacggtcaa ctgcctccga ttaacgggat ttttaaccta
     5881 atgcaagatc ctgaactaaa gctagaaaca attcacaggc aggcctcggg tgatcctatt
     5941 atcggacttt cagtacttgc gaggaaaaaa ggacacattc ctgctaaaag ttttgggaca
     6001 aagacaaaaa aactcgactc atctttaccg gaatcccacg aaataattat gagcttacta
     6061 caaaactgga gcgaagatac aatagtgctt tgcgggtaca acagcaccag agtaaaaata
     6121 aataactttg tccgaacagc tctaggcata gaatcgccgg agccaactgt aggggacaga
     6181 gtgatttgct tgcgtaacaa tcatcagaaa aatatcttta acggaatgat tggtaccatt
     6241 acttacatag cagaagaaaa cgagaccatg tattacgcag aaataatttt taacgaactt
     6301 cctgagcaat acagcggcca cattttgaaa tcacaattta acaacgaaga gtcaataaat
     6361 tacacaaaaa ataggaaagc tacattaaaa tgcgacctat ttgattttgg ttatgcaatg
     6421 actgttcata aagctcaagg cagcgaagca gaaagggtta ttttatttga agaaagattc
     6481 cccaaacttg acgatttagg ttggagaaga tggctttata cagcgattac tagggctaaa
     6541 gaggagctat atatattagg gcccgcacaa gaattgaaaa acaacaacaa tgaaaactga
     6601 ggcctaagct gacaaggcat aacttaagag ttatacttta tttgtgtcag agcaaaaatc
     6661 aaaatttgtt atttggtcgg cggtaatatt tgtaatgctt ttcatactgt cgttcttaat
     6721 ctctattcaa ttcgaaagca tagtgagctc tccctcaaga ggttttttag gattgaatct
     6781 aaacttcttc cttaatctag tagttttact tactctgcta agctttgttt atacattttc
     6841 cataggaaca tgggaatcta aatggcattt ggcgtatatt ccgctcagca ttgcgctagg
     6901 aatgtttgcc attgtcgcgc catataattt actagccgga atcatagtat tatttacagc
     6961 ttgtgtatta atttttagcg acacatataa ctcaacaaaa atgaaggaga atttaattac
     7021 attcgacccc atcttaataa tgaaaacttc tattagaaat atcttgctaa ttttttcact
     7081 tcttgcagca gttattgcca tgattaatac taaaggtaga agcgaaaacg catttgctaa
     7141 ccaaattacc acaatggcag aaacccaaat tgattcgata ctttcaaaca aagactttca
     7201 gacctatata aacaacctga cattgggtat gggcaattca ttaaacctag gccctgttga
     7261 aaaaaacata atagaaaaaa acatgccctt gaccgatctg caagacaaag ttaaccaaaa
     7321 ctcttcagat ccaaatcaaa acacagcaca ggaagaacaa ctcgcgttcc caaaggaaga
     7381 aataaaagct gtagcattag ccacgcttaa caaatatata aaaccttact cacagctgct
     7441 gtttattgct ttttcagcag ttctttttgg atttgtgcaa acctcgggaa cagccgctta
     7501 cataatttac gctatgcttg cccgcccttt cattaaactg cttcttgcgc ttggaatatt
     7561 taaagaacaa acaaaaacag ttgagcaaaa aacaattatt cttccttaaa acttaagtct
     7621 ttaacattca cagaattctc tacagaatat tctcctacct ttgtcctcac aagagacaaa
     7681 agaatgcctg aacagccaag ctgctctcct aaatcatgcg ccagtgacct tatataggtt
     7741 ccgctgctac aagagatggt acatttaaaa acgggtattt tgcgatttgc aaaaccgaca
     7801 ttgcctaaaa cttcctggcc ggattccaat ataaaatttt ttagttcggc atgtaaaaaa
     7861 gttacaggcg ttatgctata aatagttaca gttttaaccg gcaacaactt actatcaaga
     7921 agattttttc ttgctaaatc gtaaagcctt ttgccaccca cctttttagc ggaatatggg
     7981 ggtgcggttt gctcgatctc tccagtaaaa gactgcataa gttctaacac cttggattta
     8041 aaagacccca acaactgcgt tgttacaaca gggtctttat tttcaaaaga agataaaaaa
     8101 acatcattaa aatctaaggg gccttctaaa tcgtatgtag gagaatcgat gccaaaaccg
     8161 atttcacaaa catactcctt tgttgttaac atgagggaat cttgcttttt agtatctgct
     8221 cccgttaata caactagaac gccttcggct aaaggatcga gagtcccggc atgcccgact
     8281 ttaattttat tttttttggt tgagctagct gaattaatta tttttttaat gtaagcaaca
     8341 acgtcaaaac tagtccaatt tttaggttta taaacattca gtatcatcta tgagaactat
     8401 agactgtcaa ttgagcattt tcaagacaaa ttgcgccgag ataatcctca gctgatcgct
     8461 cgaaatcttt ataagactac gcaaaaggtc ttcttcttag taaagaaaca gaataagggt
     8521 aagattgaca cgccaggact tatggtgtat agagaaaact atgggaaatg gtggagaaga
     8581 tggaaatttt tttctagagc cgggttcgct ccgtaggggc gaggttgaaa aaacctatac
     8641 aacacaagag gtttttagcg catgtactcg tagacctagg cctgaagccg tttcacttat
     8701 tgacgacatt gtgagagcgg gaagtggggc tgatggcggt aaaagttgga ccttcttata
     8761 ttggtccgaa gatagatatg gccgtaagcg ttcaggaaga ttttatgcac aggcgcttgg
     8821 cgaaggagaa aaacctattg caacattaat tacggcttgg gaaccaaata ctatgctcgt
     8881 attaccacgt ccaagcggac cagtttttat aagtgggctg ggagaagttg gtgaattgtt
     8941 ggctggaagt gtctacactg taacacctga aaatcggggg ctttttccgt atattctagg
     9001 taaacatcag ttatatagat atcttggggc gatagctgat tttacgggca ataaaacacc
     9061 ggcccctttc ctagaagaag aattgttact ggagttacac cagaacacct tagaggatgt
     9121 gcggcgcgca gacgcaccct gtagaaaaag caaggcaaag agatcctggt ttgggcttct
     9181 tacggatcaa tttttagatg gattcgctgc gaatcctacg aagcccgaat gcggagtcac
     9241 cacagcaacg gcatatgcag cggcccggag cctcttgccg ctggattatt ttcttggcag
     9301 agggggggat gggtttgagt tctgcctccc acgctacgac ttagaaacta gaagacaatt
     9361 tatagttgat gcttatagat ttaacgtggc cagacagcaa agagaattaa tacaaagctt
     9421 tgcctatgct gatatgtggc gagattaatg tgggccattc caaaaagcta aattctttag
     9481 ctgttgggcg gtaattgcat aaagtccagg ggtacaaaaa aagaacaaac aattgaaagt
     9541 tgctgtagac ttctgcccga tcgtaaagta acaaacacaa ttaattattt taaaacaact
     9601 tggctaacaa gaagacaagt atatttttct aaacatatcg tacaattaag agatgagtaa
     9661 gagtgtgaat aatacaccgg tgcttaggcc ggaacaaaat gacaatccaa ctaaaatggc
     9721 ttctgcagag attgctgaat taactgcgcc tagacttatg tcctacctta aaaaggaggg
     9781 catcgaacac gaggttcgtt ccggaaagaa aaaaaatact agatataata atgcatggag
     9841 tgagatatta ttaagagaag cgagaataaa aataataact aacgtgtcga catataacaa
     9901 accggatgag actttggggc aaggcgatgg cccaggaaaa aaagggttgg tatcaactgt
     9961 ggaaataaga acagtagggc cgaatagcac ggacggcgct gtagtttggc ataactcttt
    10021 aataatcgcc gacaactcgc acaccagcgt aacgttcgta agaaatgggg aaggggagat
    10081 gtttatgagg ccgggggatg gggtagataa agtaataacc gataaaagag cacaagaaac
    10141 ataccttttg gcattgctaa aaaaatcttg catcctgaaa aaaaataaac aaaagcacag
    10201 aatatctgcc ggaaaataga ggggataatg tgactaagtt actctacaaa ataaattggt
    10261 ttgaccggag gtttaaaatg acaaaattga cagcctgcgc caggcgttac ataatatgtt
    10321 tatgacaact atttcatcta tatctggtat atctggagat caggttgccg gttttgagcg
    10381 ggcatcaatg ctggacaaac aaagaccaaa gattaaagta acgaatatta caggggcagg
    10441 tggcggtgct ttagcaacag ttgttgtagg gcatgatgaa ataggcggag agtctagcgt
    10501 aattaggttt agtgtctcat ctacagacag gaatactgcg ccagccgaga tacaagccac
    10561 cctcctagaa acttctcgag ttgcgaaaaa cgaactaaaa aatatttttt ctcgacactc
    10621 tctagggaat tctgatttaa caaacctttt actgggcgca aacatcatgg aaagagaagt
    10681 ttttgtcgtg agtgaactgg taacaggcgg acaaagtgtt ggtactgaaa caataacagc
    10741 atacttaccg ggcggaaaca ccgttagagt atctctacat gctcagggta tcagcggaat
    10801 tgatttttta ggtagtaatt gttggcagtt ggcatatagt gaaagcccct ctggtggctt
    10861 gactatagat agaggaactt ggcaaataaa gcgtgacgtc gggagcggta attgcataat
    10921 tgcacataca acatctcggc ctgaagtaca tgcaggagca gctatttcga ctcaaagacc
    10981 agtagcaaaa cctaggaatg tagtggaggc cgaaaagatt gctgcgaatg aacgtgctag
    11041 tcttacgtta agcagtgcct tatatttagt tagtcaagca ataatggcag ccaagttgag
    11101 tgccgatcaa gaagatgagg accacttctc gttaccgggc agagctactg cgtattgaaa
    11161 actaagacaa ccgcaaaaaa atttataaaa taagcacttg gacatagtca aaaaactttg
    11221 attagctggc caacttacag aattagcctc acctttgaaa tatatactaa ataaaatgag
    11281 ccaaaaattc acgaggacta aataaatagc tgtggaaaat taatatctgt atctaaattc
    11341 gattcacaat aagcaagaga gggacaaaat tagacggcag gatcgtggga gataagtttg
    11401 aatatttaaa agactgcaga aaaaacccac ctattaagga aatagcacag gccggtgttg
    11461 aagtaatgca gatattgaca ttataaaaaa tatagcgtat aggtgggata tggcgcaagt
    11521 aactccctat agtgagacac cagagcaaga acatactgta gcttatgaaa gaagcagatc
    11581 ttggaggcca gttgatgcat cacgagtgat ttcatgccgt aaagatttta atgagctaca
    11641 acaggatatt ccgaaatttg atcctgtggc gtacactctt gatggtccaa atggcaaagt
    11701 ttatatacgt gggatgcatg ggccaggaga aagactgcat gggcacgtat atactgtaga
    11761 aaccccgact cgagtatatg tattatccgg acttggacta ctttcgtatt tcagcatcac
    11821 gggagatgaa tatcaaaacc tcggggcgag ttttacagaa ttctggctga aagaaacaca
    11881 caacagtact ctacaccagg ccggaaggaa tgggaatgat cctataaaca tgtggtattg
    11941 gatggattat tttcttatgg taaatgaaag tttatataaa aataaaaatc ctactaaccc
    12001 cgcggtatgg ccagaagcac acgcattggc ccgtcaacta ctaccacgga cgcgtttact
    12061 agaatctagg gggacaaact tcttactcta ctgccgtata gctgaagatc agggaggcga
    12121 tcccatatat agggtcatgc aatatgacca gcgcaataaa gttgagacac aagttaagac
    12181 atatacctac ttaggtggtg aggttataac aagataatac actagtgaat atcaaaggta
    12241 tcctttttca aaaactacca gccacaatgt cctcttattt tatgcaaggt tgaatgtatc
    12301 gaccaactat gcccaacggg aagaattagt agtctataca atttaataac agggttgttt
    12361 ctttatcaaa atatttagca gctttataat gagccattgt ttcaactagt gaatattcta
    12421 aattaaaggc tttttctatt aatgtttcac catatcctgt ggagcatcct tttttcaatt
    12481 taatatttgg gtattgagat ataaaacttg ttaaaaactc tctaacagct tcaacaggat
    12541 tacctttatt tgattgatat cttgtatgta ataaattgtt atct
//
LOCUS       DAOSWN010000015        12559 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_3189_length_12559_cov_4.242802, whole genome shotgun sequence.
ACCESSION   DAOSWN010000015 DAOSWN010000000
VERSION     DAOSWN010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12559
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3189_length_12559_cov_4.242802"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="PLF29_01385"
     CDS             <1..1167
                     /locus_tag="PLF29_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52625.1"
                     /translation="SKEVAFFAILLFICAPFHTASLYWISAQMSLFSLLFISIAMYFS
                     IKYSIFYSTFLSYLFAFISILVYENSIALMPLIVLVILFSKESFYKKVIYVLPYPLGL
                     FSYLLFKRTALVFINATEFVKEASLIRSDSLKLMLEQITLFSNFFFGPDALKFVSDIL
                     RNYRDLIVLEYFSSVLLLLVAFLYLLYTLYVYVRKKYHSSKYLCDVPPISRWILYFSF
                     ILSFFVPVIGNYSTSSAPMGDRINLPHLLLLATLGSAIILDKARVLGLKVIFLFLLFF
                     TIMNNISLAHYIRASELQLEFAEFIKHTEISENTLINLSTDIRTYKGIPVLDNIWTRT
                     YIISNYKNLYNVETDIDYDRFSTDGSTIKYQSILSPTRFFSDPAIIDITYFRNQ"
     gene            1167..2984
                     /locus_tag="PLF29_01390"
     CDS             1167..2984
                     /locus_tag="PLF29_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52626.1"
                     /translation="MLLLGVLEDNVYHIALGNLYRIFGSNSIKFFPMYRSYPVVPYEN
                     SQPLSFDLFFFLSRFAPSIAVYNIFTLTAAIMGLIVGYKFYKAFIRNSYLSLLLSVIL
                     LGSPYFYYHARSHHDLSQYWLLLIGMYATIFYNERLEQKGWGFRSLILGALFGLVTVF
                     SNYYGFFLLMFHAIYISIYSLLYKRYKSRLLLLFKSSLISLVPFFLLGVFFLRNYLLL
                     LRTSLTDFTTPARTLEDFFYFTSRPWYFLLPPIDNPLLGKLSSSALSYLQDDWGYWLT
                     GNFFKQEHSASYIGYSVLFLSLLGVCRTLFPRGRLSFVACRKVFSLFSRTSKAYTSQA
                     RARPVYRKEVYLMHKNILISLALSTVVLFSLTMPPYFTIALHKIYTPSYVSYLMFPMF
                     RSLVRLSELINIIVLIFAGYGLLFFNSWADFTIAAKFPKMSERTRAFIISLFVFVITV
                     FAVSEKYIPLKVTKYSYPPSILSYIKENLPKDSVIAVYPFSNVNEYMFWISYTQRPII
                     NPRGFGQGSFGFESDKFTENLVSCEGMQEAIDLGVGYVLVYNISSLSSRIVDFITHNQ
                     FLKQLYVYKTNISGEDSFSQEIILYKIEKDSFLVSSCYN"
     gene            3404..5605
                     /locus_tag="PLF29_01395"
     CDS             3404..5605
                     /locus_tag="PLF29_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52627.1"
                     /translation="MIRAIFAYFIVWFLLVGSISAGGDPYEYYVDYFSNVGLDINETN
                     VLENSQNSRQNRNSSKDSYVVLLTSMIDDDDLWQAVRRPVSDTIIVCDDEASIEDLFS
                     CNRQNVFSEEFVVSVPVKNFASLVSVNLVEIDSGKYLATIIPESFLIAPKLRESLSLY
                     LQAREVARANGFEKTNVDLIEKNPPLFNVDYLFGLEVAATGALLSFVYLALGSTIKRY
                     LISPKLFLDPLLYKKALLSIISYIERVRDYITLSNIGILFVLLSLVLLISLKDVGTVS
                     LPYMFQLLYRAPSFNSFSASLSFILSMLILCWLFTSLLLQLPAAISFFLSGKSKLILL
                     PIKFNKSKVFSMILFLLPVPIVGLFSLAHIYFELYFFSVLFSLCLISLLVIESKYNIT
                     KSLSPVHKISLTALSVLSMSVVLFFVGYLDRPSSDWKYEQFIGLRDPVVMLPYYKQLG
                     KRVLVEPLNIEAPENVFMADNFVIHYPGFKKLVHKNIRDLSTESAYVFYYTKASSMAK
                     KLLEDSVDVSKVKGKEYSSLFLFDPPSLIRPEGTELKITLDCNDLDPGDDVSATIFYL
                     GSDGKLKSHTNKLVYFPGCSAIDDKQGIYDVSMTPEEFSAVPSNISREYVVPFDFPLE
                     IDGKFVVYLTGVGGSYGTKLITEFGANVLTGTGIVVPGKLSSVFNVEPSKEILVIFGD
                     YKEDVEIESKIKNGRLDVSEPINELIKKRLIVGGFNLWIPHPSGPVFIRNR"
     gene            5729..7183
                     /locus_tag="PLF29_01400"
     CDS             5729..7183
                     /locus_tag="PLF29_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52628.1"
                     /translation="MRFIKSFLLYTLLFFAPWVLLYRTGINPMPIQSEDTLPSIFTAV
                     AISKERTLYLDSYYDMLVDSYPQPDDKQQELGLTPFYLKKALDNQSGQTHYLSAFPIT
                     PAITALPVFMPPLLLGMPVSWHNLYVLSHLSSALTMAAAGVVFLYLIERYYLPGMRKK
                     AVFLTFIYLFCTINFAVVSQAMWQFGTLQLFSLLALLFFNRALEIEAPNLSAKGASSY
                     FFNIFMSGMFFGLAFITRPTAVLPWFVLLIVIYFSKYKNLGMLLGSMFTFVFGLFPAL
                     GFLIFYTGKFYRSFANQGYVEQIDVGWKSQVVNGFFGLWVSPSKGILVYSPVFLFIFV
                     SIFFLVRKIKNRNFSGMDYQYVAFISIVFMHTIVLGKWKHWYGGWSFGYRMASDVMPY
                     MVLALIPFLTSTAKEKFRKPFYLLVCVSFAVQLMGLVFFDGIWHAAYDKGYSDTSWLW
                     SIRDSEAAFNIRRMLVKIGYLERACEVCEPSVFE"
     gene            7270..8454
                     /locus_tag="PLF29_01405"
     CDS             7270..8454
                     /locus_tag="PLF29_01405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXK52629.1"
                     /translation="MTKAASLGHKVIFIDPPARFKFLKQMLKNRKWIFSKINSNLTVY
                     SPVNQFNFSPFLFLQNKIHCWLLSRYIKKQGLENMKRVLWVYHFDFPRLWDLDKNLKP
                     DVLIYDVVDAYEFFPEYARKDVTNKGVVKLIQLADRWLKEHIEQHGLGGKEWVRFREN
                     ELAKRADLMFASHPLLYKNFAEKTKRIIYTPNAGDFEIYSKKPESLPSELNNVSRPLV
                     GYSGAIDAYKFDSDLFIYLAEQTPEITYYLIGNFYISDSNPAVVKIKSLKNVMCVGSR
                     PFEVTAELTHYFDAYIIPYVINEYTYNGCFPVKVFNSLSTGCPVVVSDLPAYEGLENV
                     LYVAKDKEEFVDKVRQAVLDKDPVKAKTRVAVAENNTWDIKVSKQLKAISDFLSGKDS
                     AR"
     gene            8523..9680
                     /locus_tag="PLF29_01410"
     CDS             8523..9680
                     /locus_tag="PLF29_01410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXK52630.1"
                     /translation="MKILYTLDSANIGGMEKYVLDLAEGMMSRGHEVHIWCRQGPLAK
                     IYQNAGAVVVPVEVKYDIDPLYIYRQIKYIKKHNIQVVHCNELRAVANTLIASRLAGV
                     RCRISHIHTPYSQWQVPVYKRKIYSICYAMAVRLLSSSEIALTNETLKVKLEEGIPLS
                     KLKIIGNSVNFDALKLNESQKNKYRAEILKKHGLPEDIFIIGNISRLTEEKGHRFLVS
                     AFANLLESHPELSKSHLLVAGGGYLQESLEQQAKDLGISNKVTFTGRFIEEDKIKYLA
                     SFDMFIFPTLAEGFGIVLIEAMYMYIPLICSDLPVLKEVSKGTVKYFESGNSEDLCVK
                     MYELFHSLDGLNTKERVVAAHTVVRKNYSLEDFVSSYEDLYKKCVRKKESE"
     gene            9689..10822
                     /locus_tag="PLF29_01415"
     CDS             9689..10822
                     /locus_tag="PLF29_01415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXK52631.1"
                     /translation="MQARVELFDTGGGDKIQIENTARELKKLGIDVEIVAGNVSNYNS
                     YDLVHLFQLDWTPDPSMYAETIKSSGKPLVFSPIHHSISEVKLFDETYAFDFRRLSKA
                     VFKDQFKRDTLKNVYKSLFMPKKIYPTVQSLRFGLFNIQKKALSLSDYILVQTELEAH
                     DLKTSYGVDFKWKKIPNGVSDLFIDSAGYDKEQIMPKALQDMEDYILCVGRVEARKNQ
                     LSIIDAVKSAREETGKDLRLVFVGKKSKLKHQEYIIRFNLALKRYSWITFVNQVPYEQ
                     MPAVYRKAKVCVSASWFETTGLTSIEALFCGSNAVASGERAREYLGDLVSYCSPNDIR
                     SIKNAVVEQYFADRPKVPSFMLREYTWQNAAHKIFDVYNELLK"
     gene            10824..12536
                     /locus_tag="PLF29_01420"
     CDS             10824..12536
                     /locus_tag="PLF29_01420"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HXK52632.1"
                     /translation="MSIELLSLFGITLLSLLLFKLDIEKGMKIILLCSVLLHKELFSF
                     YSWNLLPIRFVMLAFSAYATLYFIKNYKKLAALISDPFVLSLGLLFLARLISISNTKN
                     LGASVSLLAFFATACAFVIFTYSVYKDKPDKILEFIKYYINVAFYLCVFGFIQVLVYS
                     RFDFIMGALWNIPGHTPRVGSLFWDTNHFGGFLTGLLPVTGVLFLFAETYKERLKYFI
                     YLVSMSAILLLTSSRTAWIAGFVSLLIFGMLLIYRKLHLRGVLALVFTLFLLTIPLVR
                     EYNIKSSPFRAKVKDYFHYRIDSFDSHVLLLKGSVEIFEKYPLIGGGYGGFFEHFSKT
                     EIASEFFGRDPAAFNTRVPPHTIWGELLSETGALGLAAFVAVIGLMLSAMLYASQKLK
                     NAKTYFTVSAMVGAIVGWLTAGIFYSYNNEFFWIILAFYFVYTIGVLGKDFTFSKAVN
                     YVFDSSRSQFFGLFVLGVVVFFTKLGSNHLIPWDEAIYAKIAKNIYQTGDYIFLKWSS
                     SELWLEKPPLYMWLMALSMKIFSITSFAVRLPSALFGLGTVLLTYVFGTKLYGRLAGF
                     FKGE"
BASE COUNT         3434 a         2164 c         2468 g         4493 t
ORIGIN      
        1 agcaaggagg ttgctttctt tgcgattctt ctgttcattt gcgccccttt ccataccgca
       61 agcctttact ggatatctgc ccagatgtct cttttctctt tgttatttat atctatagca
      121 atgtacttca gtattaaata tagtatcttt tatagtacat ttttatccta tttattcgca
      181 tttatttcta ttctcgtata tgagaattct attgctttga tgccgctgat agtgttggta
      241 attttattct ccaaagagag tttttacaaa aaggtcatct acgtattacc ttatccccta
      301 ggacttttta gttatttact gttcaagagg actgcgcttg tttttattaa tgctacagag
      361 tttgtaaaag aggcctctct aataaggtcc gatagtttaa aactcatgct ggagcagatt
      421 acgttgttta gtaatttctt ctttgggcca gatgccttga agtttgtttc tgatatcttg
      481 aggaattacc gcgatctgat tgtcttagaa tatttctctt ctgtgctctt gttgttggtc
      541 gcctttcttt atcttttgta tacgctttac gtctatgtga gaaagaaata tcacagcagt
      601 aaatatttgt gcgatgtgcc accaatttct cggtggattt tgtatttctc atttattttg
      661 tcattttttg tcccggtaat aggtaactac agcacaagct ctgcgcctat gggagataga
      721 ataaacctcc cgcaccttct tttgctagcg acattaggtt ctgctattat cttggataag
      781 gcaagagttt taggtcttaa agttattttt ctctttttac tgttttttac tattatgaat
      841 aacatttctc ttgcgcacta tatacgagcg tctgagctgc aactagagtt cgcagaattt
      901 attaagcata cggaaatttc agaaaatacc ctgatcaatc taagcacaga tattagaact
      961 tacaaaggca ttcctgtgct tgacaacata tggacaagaa cttatataat ctctaattat
     1021 aaaaatcttt ataatgttga aactgatatc gattatgatc gtttttcgac agacgggtca
     1081 actattaagt accagtctat actttctcct actcgctttt tctcggatcc ggcaattata
     1141 gatattacat attttaggaa tcaatagtgt tattacttgg agtgctcgaa gataatgttt
     1201 atcacatagc cctgggcaac ctttatagga tttttggctc taacagcata aaatttttcc
     1261 cgatgtatag atcttatcct gttgttccgt atgaaaattc tcagcctttg agcttcgatt
     1321 tatttttttt tctatcgaga tttgcacctt caatcgcggt ttataatatc tttacactga
     1381 ccgcagctat tatgggtctt atcgtgggct ataaatttta caaggccttt attagaaatt
     1441 cttatctttc gttattactt tcagtaattc ttcttggcag tccgtatttt tattatcatg
     1501 cgcgaagtca ccacgacctt agccaatact ggctgttgct tataggcatg tatgcgacaa
     1561 tattttataa cgaaagatta gaacaaaaag gttgggggtt tagatcttta atattagggg
     1621 ccctgtttgg gctagtaact gtttttagta attactatgg attttttttg ctcatgtttc
     1681 acgctattta catatcgatc tattccttat tgtataagcg ttataaatca cggcttctct
     1741 tattattcaa aagttctttg atatctttag tacctttttt tcttctgggt gtattttttt
     1801 tacggaatta tttacttcta ttaaggacct ctctaacgga ttttacaact ccggccagaa
     1861 cgttggagga ttttttttac ttcacttcaa gaccttggta ctttttactt ccaccaatag
     1921 acaacccctt attgggaaag ttgtcttcga gcgctctttc ttatctgcaa gatgattggg
     1981 gatattggtt aacaggaaat tttttcaaac aggaacattc cgcaagttat ataggatatt
     2041 ccgtcctatt cctatcgctt ctgggagtgt gtaggacttt atttcctcgt ggtcgtctta
     2101 gttttgttgc ttgtcgtaag gttttttctt tgtttagcag aaccagtaag gcttatacca
     2161 gtcaggcccg agctcgccct gtttatcgta aggaggttta tctgatgcac aaaaatattc
     2221 ttatttcgct tgcactttct acagttgtgt tattttctct cactatgcct ccgtatttca
     2281 ctatcgcact acataaaatt tatacaccaa gttacgtgtc gtatctcatg tttccgatgt
     2341 ttaggtctct ggttagactc tctgaactaa taaacatcat agttttaata tttgcaggct
     2401 atggccttct tttttttaat tcatgggcag attttactat agcggcaaag tttccaaaaa
     2461 tgagtgaaag aaccagagcc tttataattt cactattcgt ttttgtgatt accgtttttg
     2521 cggtctccga aaaatacatt ccattaaagg ttacaaaata ctcatatccc ccgagcatcc
     2581 tttcttatat taaggaaaat ttaccgaaag attcagttat agctgtatac ccctttagca
     2641 atgttaatga atatatgttt tggatttcat acacccagag gcccataatt aacccccgag
     2701 gtttcgggca aggtagtttt gggtttgagt ctgataagtt cacggaaaat ctagtttcct
     2761 gtgaaggaat gcaggaggcc attgatttgg gcgtggggta tgttttggtt tacaacattt
     2821 cgtcgttgtc ttcgcgcatc gtcgatttta ttactcataa tcaatttcta aaacagcttt
     2881 atgtttataa aacaaacatt tctggagaag atagtttttc ccaagaaata attctctaca
     2941 aaatagaaaa agatagtttt cttgtcagta gttgttataa ttaaggtatg tgttccttta
     3001 ctcagctgtt agagggaaaa tctttttttt accttttttt aactttttta ccttttttta
     3061 tattaatacg ctctttagat atgcgtcgaa gtatcgtgca gctcgcaaac tatattatat
     3121 cgggatacat cggtgttata ttttacattt cggtttgcaa tcttattcgc tttgactttg
     3181 taaatatttt actaattgcg gctcttattt tcgtactttc tttatttctc ttgtttttgt
     3241 tttttgtgga tatgtggtat ttaaattttg ttctatattt tgttgcattt gcactgcctc
     3301 ttgtttggat aacaggctct ttttctttat tcagattgct ggcgctggtg tttgtgcttg
     3361 tttctacctg tctatttatt agaaattatc taagggggaa aatatgatta gggcaatttt
     3421 cgcctacttt atagtatggt ttttattagt aggatccata agtgccggcg gggatcctta
     3481 tgagtattat gttgattact tcagcaatgt tggtctggat ataaatgaaa caaatgttct
     3541 cgaaaactct cagaatagcc ggcaaaatcg aaattcttca aaagacagct atgttgtatt
     3601 actaacttct atgatagatg acgacgactt atggcaagcg gtgcgccgtc ctgttagcga
     3661 cactattata gtttgtgatg atgaagcttc gattgaagat cttttctctt gcaatcgtca
     3721 gaacgttttt tctgaagaat tcgttgtctc tgtcccggtt aaaaatttcg catctctcgt
     3781 ttcagttaat ttggtggaga ttgattcagg taagtatctg gccacaataa tacctgaatc
     3841 ttttctgata gccccgaaat tacgtgagtc cctctcgctc tatctgcagg caagggaagt
     3901 tgcgagagca aatggttttg agaaaacaaa cgtagatctt atcgagaaga accctccttt
     3961 gttcaatgtc gactatctct tcggtctcga agtcgcagcg acaggagcac ttctctcctt
     4021 tgtttatcta gctttgggca gtacgattaa gagatattta atttctccca aactctttct
     4081 tgatcccttg ctttataaaa aagctttgct gtcgataatc tcatacatag agcgcgtaag
     4141 agattacata acactcagca atattggaat attgtttgtt ttgctgtcat tagtgttgtt
     4201 aatttcatta aaagatgttg gcacggtaag cctgccctat atgtttcagc ttttatacag
     4261 ggccccgtct tttaactctt tctcagcctc actttctttt atactctcta tgcttatatt
     4321 gtgttggttg tttacctcgc tattattgca gctccccgcc gccatctcgt ttttcttatc
     4381 cggaaaatct aaattaattt tattaccaat aaaatttaac aaatcaaagg tattctctat
     4441 gattttattc ctgcttcctg tgccgatcgt cggactcttt tctcttgccc acatttattt
     4501 tgaattgtat tttttttctg tcttgttctc gctatgcctc atttctctgc tagttattga
     4561 atcaaaatat aatataacta aatcactgtc tccggtgcac aaaatttctc tgacagcgct
     4621 gtccgtatta tctatgtcgg tcgttttgtt ttttgtagga tatctcgatc gccccagcag
     4681 tgattggaaa tatgagcagt ttattggttt gcgcgatcct gttgtcatgc tcccgtacta
     4741 taaacagctc ggaaaacgcg ttctggtgga gcctttaaac attgaagctc cggaaaacgt
     4801 ttttatggca gataattttg ttatacatta ccctggtttt aaaaaactcg tgcacaaaaa
     4861 cataagagat ctctctacag aaagtgcgta tgtcttttac tacaccaaag ctagttctat
     4921 ggccaaaaag ctactagaag atagtgttga cgtatcgaaa gttaagggca aagagtattc
     4981 ctcattattt ctttttgacc ctccctctct cattagaccg gaaggcacag aattaaaaat
     5041 aaccttagat tgtaatgact tggatccggg cgatgatgtt tcagcaacaa ttttttatct
     5101 tggcagcgat ggtaaattaa agtctcatac gaacaagtta gtttattttc ccggctgttc
     5161 ggccatagac gataagcagg gaatttatga tgtttcaatg accccagaag agttttccgc
     5221 agtgccgtct aacattagtc gtgagtatgt tgtgcctttt gatttccccc tcgaaattga
     5281 cggcaaattt gtagtttatt tgaccggtgt tggcggtagc tatggtacaa aattgattac
     5341 cgagttcggg gccaatgttc tgacgggtac cggtattgtt gttccgggaa agctgagtag
     5401 tgtatttaat gttgaaccct ctaaagaaat actcgtgata tttggtgatt acaaggaaga
     5461 cgtagaaata gaatcgaaga taaaaaacgg taggttagat gtcagcgagc ctatcaatga
     5521 gctaataaaa aagagattaa tagtgggagg ttttaatctt tggattccac atcccagtgg
     5581 cccggtgttt attagaaatc gctgatttta tatgtgtgtt gttaaaaata gctaaaccga
     5641 cgatcgggca gaggtttgaa ttttctgttg ctttaggatg catgagaatg cacgtgcgat
     5701 tcgtctgaaa acattgtaga ataagctaat gaggtttata aagtcttttc ttttatatac
     5761 tttgcttttt ttcgctccgt gggtattact ttatagaaca ggcataaatc caatgccaat
     5821 tcaatctgaa gatactcttc cctctatttt cactgctgtt gcaatttcaa aagagagaac
     5881 tttatatcta gattcatatt acgacatgct ggttgattcg tacccccagc ccgacgacaa
     5941 gcagcaagag cttggattaa ctccttttta tttaaaaaaa gctctcgata atcaatccgg
     6001 tcaaactcat tatttatcgg cttttccaat aacccccgcg atcacggcat tgcccgtttt
     6061 tatgccccca ctgctgcttg gcatgccggt aagttggcat aatttatacg ttctttcaca
     6121 cctttcaagc gctttaacta tggcggcggc aggagttgtt tttttatatt tgatcgaaag
     6181 atattatttg ccgggcatgc gtaaaaaagc tgttttttta acatttattt atttgttttg
     6241 tactattaat tttgcagttg tttctcaggc aatgtggcag tttggaactt tgcaactttt
     6301 ttctttgctc gctttgctat tttttaacag ggcattagag attgaagctc cgaatcttag
     6361 tgctaagggt gcgagctcat atttttttaa catctttatg tccggaatgt ttttcggatt
     6421 agcttttatt accaggccta cagctgttct tccgtggttc gtgcttctta tagttattta
     6481 tttttctaag tacaaaaatc ttggaatgtt gctgggatcg atgtttacat ttgtttttgg
     6541 tttatttcct gctctaggat ttcttatatt ttacaccggg aaattttata gatcgtttgc
     6601 taatcaaggt tacgtagagc aaattgatgt gggctggaag tctcaagttg taaacgggtt
     6661 ttttgggttg tgggtatctc caagtaaggg tattcttgtc tattctcctg tttttttatt
     6721 tattttcgtg tcaatattct ttctagttag gaaaataaaa aatagaaact tttcgggaat
     6781 ggactatcag tatgttgctt ttatttcaat tgtttttatg cacacaatag ttttaggtaa
     6841 atggaaacat tggtatgggg ggtggtcttt tggttataga atggcgtctg acgtcatgcc
     6901 ttacatggtt ttggctttaa tacctttttt aacttctaca gccaaggaga aatttcgtaa
     6961 gccgttctat cttttagttt gtgtttcatt tgcagtacag cttatgggat tggtattttt
     7021 tgatggaata tggcatgccg cttatgataa aggttactcc gacacctcct ggttgtggtc
     7081 aattagagac agcgaggcgg catttaacat aagaagaatg ctagtaaaga taggttatct
     7141 ggaaagggcg tgcgaggttt gcgagccgtc tgtctttgaa tagctatgtc ttctgatgaa
     7201 aaatatttaa taatatgtct gtccaatcaa ttttttgata taccttataa aactaataag
     7261 cacttggtaa tgacaaaggc agcatcttta gggcataagg ttatttttat agatcctcct
     7321 gctcgtttta aattcttaaa acagatgttg aaaaatagaa aatggatttt ttcaaaaatt
     7381 aactctaatt tgactgtgta ttcccctgta aatcaattta acttctcgcc gtttttgttt
     7441 ttgcaaaata aaattcattg ctggctatta agtagataca taaagaaaca ggggctggaa
     7501 aatatgaaaa gggtattgtg ggtttatcat tttgattttc ctaggttatg ggatttagat
     7561 aaaaatttaa aacccgatgt gttgatttat gatgttgttg atgcgtacga attttttccg
     7621 gaatatgcaa gaaaagatgt tacaaataaa ggcgttgtaa aattaattca acttgcagac
     7681 cgctggttga aagagcacat tgaacagcac ggtttgggcg gaaaagagtg ggttcgtttt
     7741 cgtgaaaatg agttagcaaa gcgtgctgat ttgatgtttg cctcgcatcc tttgctttac
     7801 aaaaactttg cagaaaaaac aaaacgtatt atatacaccc caaatgcagg tgattttgaa
     7861 atatattcta aaaaaccaga atctcttcct tctgaactaa ataatgtgag ccgtcctctt
     7921 gtaggatata gcggagcaat agatgcatat aaatttgact ctgatctttt tatatacttg
     7981 gctgaacaaa ctcccgaaat aacgtattac ttaattggaa atttttacat ttcagattca
     8041 aaccccgccg ttgttaagat taaatcttta aagaatgtta tgtgtgttgg atctagacca
     8101 tttgaagtta cggcggagtt aacccactat tttgacgcat acataattcc ttatgtgata
     8161 aacgaatata catataacgg atgttttccg gtaaaggttt ttaattctct ttccacgggg
     8221 tgtcctgttg tagtttctga tcttccggct tacgaaggtt tggagaatgt tttgtatgtt
     8281 gctaaagata aagaagagtt cgtggacaag gtgaggcagg cagttttaga caaggatccc
     8341 gtaaaagcta aaacgagggt ggctgtagca gaaaataata cttgggatat aaaagtatct
     8401 aaacagttaa aagcaatatc agattttcta tctggtaaag actctgcccg gtaagtttag
     8461 ccttcggtct cgaaattgtg acattttata agaagtaatt aactatcagg tataataaat
     8521 aaatgaaaat tttatacaca ttggattcag caaatatagg tgggatggag aaatacgtcc
     8581 tggacttagc cgagggaatg atgtctagag gccatgaggt gcacatatgg tgtcgacagg
     8641 gacctctagc taagatatac cagaatgccg gagcagtcgt cgttccggta gaagtaaaat
     8701 acgacataga tccgttatat atttacaggc agataaaata cataaaaaaa cataacattc
     8761 aggttgttca ttgcaatgag ttgagggctg tagcaaatac tcttattgcc tccaggcttg
     8821 caggagtccg atgtcgtatc tctcacattc atactccata ttcccagtgg caagtgcctg
     8881 tctataaaag aaagatatat agcatttgtt atgccatggc tgtaagacta ctttcttctt
     8941 ctgaaattgc actgacaaat gagactttaa aagtaaagct tgaggaggga attcctctgt
     9001 cgaagttaaa gattataggt aattccgtta attttgatgc gctgaagcta aatgaatccc
     9061 agaaaaataa atacagggct gagattttaa agaaacatgg gcttcctgag gatattttta
     9121 ttattggtaa tataagccgt cttacggaag agaaaggtca taggttttta gtgtcggctt
     9181 ttgcgaattt gttggagtca catcctgagc tctcaaagtc acatctgctg gttgcgggcg
     9241 gaggttattt acaagagtct ctagagcagc aggcaaaaga tctaggtatt tctaacaaag
     9301 ttacttttac aggacgattc attgaagaag ataaaattaa atatttagct tctttcgata
     9361 tgtttatttt cccgacttta gcagaaggtt tcggtattgt tcttatagag gcaatgtaca
     9421 tgtatattcc tctcatatgt tctgacctac ccgtcttgaa agaagtttct aaaggtacgg
     9481 ttaagtattt tgaatctggg aacagcgagg atttatgtgt aaaaatgtat gagctttttc
     9541 atagccttga cggactaaac acaaaagaga gagttgttgc cgctcatact gtggtaagga
     9601 aaaattacag cttagaagat ttcgtgagta gctatgaaga tttgtataaa aaatgtgtaa
     9661 gaaagaaaga atcagaatga aagttttaat gcaggcgcgt gtagagcttt ttgatacggg
     9721 cggcggagat aaaattcaaa ttgagaatac ggcccgagag ctgaaaaagc taggtattga
     9781 cgttgaaatc gtagccggca atgttagtaa ttataattct tacgatcttg tccatctttt
     9841 tcagctcgac tggacccccg acccgagtat gtacgccgag acgataaagt cgtctggtaa
     9901 gccgctggtg ttttcaccaa ttcaccactc aatttctgag gtaaaattat ttgacgaaac
     9961 atacgccttt gattttagaa gactctctaa ggctgtattt aaagatcagt ttaaaagaga
    10021 tacattgaaa aatgtttata aatcactttt catgccaaag aaaatttatc ccactgttca
    10081 aagtttacga ttcggtttat ttaatattca gaaaaaagct ttgtctttat cggattatat
    10141 tttagtacag accgagctgg aggcccatga tctaaaaacc tcttacggcg tagattttaa
    10201 atggaaaaaa atacctaatg gagtttctga tctattcata gactctgctg gctacgataa
    10261 agaacaaatt atgccaaagg cgttgcagga tatggaagac tatattctgt gcgttgggcg
    10321 agttgaagca aggaaaaatc aactaagtat aattgatgct gtaaaatcag caagagaaga
    10381 gacaggcaag gatctgagac tggtttttgt cgggaaaaaa agtaaactca agcaccagga
    10441 atatattata agatttaacc tcgctctaaa aagatatagc tggataacct ttgttaatca
    10501 ggttccttat gagcaaatgc ctgcggttta tagaaaagcg aaggtgtgtg tttcagcaag
    10561 ttggtttgag actacagggc tcacaagtat cgaagctctt ttctgcggtt ctaatgcagt
    10621 agcttcgggc gaaagagcta gagagtatct tggcgatctt gtgtcttact gcagcccaaa
    10681 tgatattaga tcgataaaga acgctgtagt tgaacagtat tttgcggata gaccaaaggt
    10741 gccgtctttt atgctgcgcg agtacacttg gcaaaacgcg gctcataaaa ttttcgacgt
    10801 gtataatgaa ttactgaaat aatatgtcta ttgaacttct ttctttattc ggaataacct
    10861 tgctatcctt acttcttttt aaactggata ttgaaaaggg aatgaagata attctgctat
    10921 gctcggtctt acttcacaaa gagcttttta gtttttacag ctggaattta ttgcctataa
    10981 gatttgtgat gttagctttt tctgcttatg ctactttata ttttattaaa aattataaaa
    11041 aattagccgc tcttatttct gacccctttg ttttatcttt aggactcttg tttttagcca
    11101 gacttatttc tatttcaaac acaaaaaatt taggtgccag tgtatcttta ttggcatttt
    11161 ttgctaccgc ctgtgctttt gtgatattta cttacagtgt ttataaagat aagccggaca
    11221 aaatcctaga atttattaaa tactatatta acgtcgcctt ttacctttgt gtttttggat
    11281 ttatccaagt gcttgtttac agtagattcg attttattat gggggctttg tggaatattc
    11341 ccggccatac tcctagagta ggctctcttt tctgggacac aaatcacttt ggaggctttt
    11401 taacagggtt acttcctgtt accggagttc tttttctttt tgctgaaacc tacaaagaga
    11461 gattaaaata ttttatttat cttgtttcta tgtcggctat attgctactt acaagttctc
    11521 gaaccgcttg gattgcaggt tttgtttcgc tacttatttt tggcatgctt cttatttaca
    11581 gaaagctaca cttaagaggt gttcttgcgt tagtttttac tctttttctt ttgactatcc
    11641 cgctggttag agaatacaac attaagtcga gcccttttag agcaaaggtt aaagactatt
    11701 tccattacag gatagattct tttgattctc acgtactttt attgaagggg agcgtggaga
    11761 tattcgagaa atacccctta ataggtggcg gctatggtgg gttttttgag catttcagca
    11821 agacagagat tgcttctgaa ttttttggac gtgacccggc tgcatttaac acccgcgttc
    11881 ctcctcacac aatttggggt gaattgttgt ccgaaacagg cgctctaggt ttagcagctt
    11941 ttgttgcggt tattggtttg atgttgagcg caatgctcta tgcaagccag aagttaaaaa
    12001 atgcaaaaac atattttaca gtttcagcca tggtcggggc tattgtcggc tggttaactg
    12061 cgggtatttt ttattcttac aataatgaat ttttttggat tattttagct ttttactttg
    12121 tttacaccat cggagtttta ggaaaagatt ttacattttc aaaggctgtt aattacgttt
    12181 ttgattcttc tcgaagtcag ttttttgggc tttttgtttt aggcgttgtt gtatttttta
    12241 caaagctagg ctctaatcat ctaatccctt gggatgaggc aatttatgca aaaatagcta
    12301 aaaacattta tcagaccggg gattacattt tcttaaaatg gagttctagc gagctgtggc
    12361 ttgaaaagcc ccctttgtat atgtggctca tggctttgag catgaaaata ttctcgataa
    12421 caagttttgc ggtgcgttta ccttcggcat tgtttggtct cggtacagtt ttgttaacat
    12481 atgtttttgg cacaaaactt tatggaaggc ttgccgggtt ttttaaaggt gaatgaactg
    12541 tcttcgtcca ttttgccct
//
LOCUS       DAOSWN010000016        12416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_3233_length_12416_cov_4.824933, whole genome shotgun sequence.
ACCESSION   DAOSWN010000016 DAOSWN010000000
VERSION     DAOSWN010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12416
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3233_length_12416_cov_4.824933"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..1280
                     /gene="mnmA"
                     /locus_tag="PLF29_01425"
     CDS             111..1280
                     /gene="mnmA"
                     /locus_tag="PLF29_01425"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HXK52633.1"
                     /translation="MSVLRTNPTGKKYKVAVGMSGGVDSSVAAHLLVEQGFDVTGVFI
                     RCWEAKADGCRADEDRADAVAVATKLGIPFVTLDFISDYKNKVIENFYQEYSKGRTPN
                     PDVMCNKEIKFGMFLDWALAEGFDFIATGHYAGVSSVPEVAKATSTTSTGSAELYLLQ
                     GSDASKDQSYFLYTLKQEHLKKVMFPLENLKKEQVRSIAKKLNLQTYDKPDSMGICFI
                     GEVDIKEFLHKRISFHKGLVVDEQGEIIGEHDGIEFYTVGQRHGFRINRYVGLPLYVV
                     GKNVEKNELIVGYAAQANVSIFSVEDLSWVGSVPAFPLKCDVRVRHLGEMHDCRVISS
                     SSKSGASGTTTEHKMENSLLVTLEDPIFGVAPGQSAVFYSGNKVLGGGIIGFTQR"
     gene            complement(1310..2530)
                     /locus_tag="PLF29_01430"
     CDS             complement(1310..2530)
                     /locus_tag="PLF29_01430"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXK52634.1"
                     /translation="MGIRDKQPFEYYSGHNGIDFKLAFGTPIKAPAEGNATYYFCNSC
                     GHTIKIDHLNGYQTIFMHLQGEGLFTSSSSPIHVNEGEVIGYVGLSGKTTGPHLHLAV
                     LKDKNNNNSFADDIPDGFIDPFSWWADYIQDPWPNFSWQDVLGAHKGTVSPYLWKEDF
                     SPSTKFTNNAEVQVSYYNRSLELPQNATKSGVTIRLSPLGPVGRTPENLYPVPKTGTV
                     IDIIDTLGKKIYEITAPATITIQLNGVDLANYNADTMAIYHFNAEEQIWEILDSVFDP
                     INKIITAHTSSFSAFSVLAEKYDIQAPRSIAMLSTNPLNGWFVGPTNFTVTTNEVADK
                     IYYKIGGEVEWKEYTGSSVSLEVPGSYAIQTMAVDFEGNYETPQEVYIRYNPNRKPTH
                     TNKALGTSFSIANY"
     gene            complement(2713..3654)
                     /locus_tag="PLF29_01435"
     CDS             complement(2713..3654)
                     /locus_tag="PLF29_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52635.1"
                     /translation="MKNIAICVIAFMLLSLNNNLLASESWEQLDPKIKNIINTPYGLI
                     AGEFDDRIWLNPYNGIYVSHDLGNTWIKTNGLEGRGVTTMSFDGINTIYATTAFEIDG
                     TGGIFASSDGGYTWQHLSDVFPFSGGIIKAKENLIIVGKRYNGAYFSFDKGQNWERPM
                     PDETNTKTIFSISMIGEKIYISMASNNYFSADNGHTWEEIIGLRNRRISSFAEYENRT
                     YAGDDYLGLRYLVSGTDVWSYDSFFDGYAVKDMIVINENLYVAVNDIYGNAFVFKKSG
                     VDEGWESTSGNTITKVDKLTHVYSAPQLLLGTHTNRA"
     gene            3965..5296
                     /locus_tag="PLF29_01440"
     CDS             3965..5296
                     /locus_tag="PLF29_01440"
                     /inference="COORDINATES: protein motif:HMM:NF013978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis-aminopropyl spermidine synthase family
                     protein"
                     /protein_id="HXK52636.1"
                     /translation="MLSLSKIYSSNPDLRKAQVKGLLFLLNSEDSISNNELVQRTGLS
                     KEVLKTFKESISSILIKTTADELALTKVGREAVGEMNPEPYSWTYLSFSFDALMLQAL
                     EAIKAIREGQQQFVAKREFDQFFCTPETSILKAKAILNKVGGSEGVSIALMGDDDLVS
                     ISLGLLDRAVEEIIVFDVDKDVLSLIKEKAEELKITSIKTVLYDARDDLPKDMVARFD
                     AVLTDPPYTRPGVAVFLDKCITLCKKATTYAGPYIFFCYGNGLRDPLKGLKVQDIILS
                     RNLLIEEKISTFNRYDGAEIIGSSSDLFVLKVLETTEVLEDYTGTEIYTYENTEIEDF
                     PYVDHYVFKLYKVPSHVVTSKKTLQKNIGVFCKWHGLNVVDTKLTRFKGGGFSFTYIL
                     STSNLLVHTWPEKRALHIDLITCSPIKKKHLLLKNLSDLFSTMSVEIRKIE"
     gene            5549..7026
                     /locus_tag="PLF29_01445"
     rRNA            5549..7026
                     /locus_tag="PLF29_01445"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            7494..7637
                     /locus_tag="PLF29_01450"
     CDS             7494..7637
                     /locus_tag="PLF29_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52637.1"
                     /translation="MQGGFREGGRNLITDKAVEEASVIVGSLEMKLSNLHVKAEVEDG
                     GGY"
     gene            7621..8082
                     /locus_tag="PLF29_01455"
     CDS             7621..8082
                     /locus_tag="PLF29_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52638.1"
                     /translation="MGGDINAHAAVLDQLGQSAVAENPTALEEAAEGEAAGLVGQALD
                     GIGVHEIIVNLEEIAVPTTPDTYMSDAVREQRMNAAAGLVVSALTEQGWLVHPNNPLG
                     VSQSGLRANEALSAELGLRPRAPDAAPDSLKRFVVFFKVSLIYAEPDAPSA"
     gene            complement(8164..9129)
                     /locus_tag="PLF29_01460"
     CDS             complement(8164..9129)
                     /locus_tag="PLF29_01460"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXK52639.1"
                     /translation="MTGITGFIGKRLAQRLIEQGYEVSGMHRINSNIDLLKVPAQAKL
                     FVADLNNQRSMENAIKEANPEVIVHLAALSSGGLSFNFPEEYLQTNEIGTFKLLSAIK
                     NLKGSQDINTAKLVFAGSMEVYGYQNSNEPFSETLELGPASSPYGLSKQNAERVIEYF
                     AQAMPDVKMVIMRTANTYGREGAGFYLIEYIIDSLLNKNDPKIKQPLDVRDFIFIDDH
                     IDAYVKAIEKDCEGIFNIGAGLQLNNGQLQETISKLMKIDNKKPGQRLQKQKPTSNDR
                     PKQECFLSVRYEKASKNLGWSPLTTLDEGLRKTIKMREYFFKTKN"
     gene            complement(9168..9244)
                     /locus_tag="PLF29_01465"
     tRNA            complement(9168..9244)
                     /locus_tag="PLF29_01465"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9208..9210),aa:Ile,seq:gat)
     gene            9591..9666
                     /locus_tag="PLF29_01470"
     tRNA            9591..9666
                     /locus_tag="PLF29_01470"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9624..9626,aa:Ala,seq:tgc)
     gene            9827..>12416
                     /locus_tag="PLF29_01475"
     rRNA            9827..>12416
                     /locus_tag="PLF29_01475"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         3473 a         2386 c         2934 g         3623 t
ORIGIN      
        1 gtagaatata agagattttt acactctcag taatttttag actacagagt caacttttcc
       61 taaattgttt tttctacgta aatacgtttt tatttggtat tatttcgatc atgagtgttt
      121 tgagaacaaa ccctactgga aaaaaatata aagtggctgt tggcatgagc ggaggcgtag
      181 actcttcagt agccgcgcat ttgctggtag aacaaggttt tgatgtcacc ggagttttta
      241 tacgctgttg ggaggccaag gctgacggct gtcgtgctga cgaggaccgt gcagatgctg
      301 ttgctgttgc aaccaagctt ggaattcctt ttgttactct agattttatc tcagactaca
      361 aaaataaggt tattgaaaac ttttatcaag aatattcaaa aggccgtaca ccgaaccctg
      421 atgttatgtg taataaagaa ataaaattcg gcatgttttt ggattgggcg ttggctgaag
      481 gattcgattt cattgcaacc ggtcactacg ccggagtgag ttcggtgccc gaggttgcca
      541 aagcaacttc aacaacctct actggcagtg cagagttgta tctgctacag ggatcagacg
      601 cttctaaaga tcaatcatat tttttgtata ccttaaaaca agagcatttg aaaaaagtta
      661 tgtttccttt ggaaaattta aaaaaagaac aagttaggtc aattgcaaaa aaacttaatt
      721 tgcaaaccta cgacaagccc gatagcatgg gaatttgttt tatcggtgag gtagatatta
      781 aagagttttt acacaaaaga attagttttc ataaaggctt ggtagtagat gaacaggggg
      841 aaataatagg ggagcatgac ggaattgaat tttacaccgt tggacaacgc cacggtttta
      901 ggataaacag gtatgttggg ctgcccttgt atgtagttgg aaaaaatgtt gaaaaaaacg
      961 agttgatagt gggctacgcc gcacaagcaa acgtttctat atttagtgtg gaagatttgt
     1021 cttgggttgg aagcgtgccg gcctttccct taaaatgtga tgttagggtg cgccatcttg
     1081 gagagatgca cgattgccgg gttataagtt cctcctcgaa atccggtgct tcgggcacaa
     1141 ccacagagca taaaatggaa aattcacttc ttgtaacatt agaggaccct atttttggtg
     1201 tggctccggg acaaagcgcc gttttttatt caggtaacaa ggttttgggc ggcggtatta
     1261 ttggttttac tcaaagataa tcgccccctg ggttttatgc cggcttgttt tagtagtttg
     1321 cgatgctaaa acttgttccc aaagccttgt ttgtgtgtgt gggttttcta tttgggttgt
     1381 atcttatata cacttcttgt ggcgtttcgt aattaccttc aaagtctacg gccattgttt
     1441 gtattgcata agaaccgggt acctcaagac tcacagaaga ccctgtatac tctttccatt
     1501 ccacctcgcc tccaatttta taatatattt tatcggcaac ttcatttgtt gtgacagtaa
     1561 agtttgtcgg tccaacaaac cagccattaa gtgggtttgt gctaagcatt gcaatcgatc
     1621 ttggggcttg tatgtcatat ttttcggcaa gaacagaaaa agccgaaaac gaagaagtat
     1681 gtgctgtgat gattttgttt ataggatcaa agaccgaatc taatatttcc catatctgtt
     1741 cttcggcgtt gaagtggtag atagccattg tgtcagcatt atagttagct aaatccacac
     1801 cgtttagctg aatggtaatg gttgcgggcg cagtaatctc gtaaattttt ttacctaagg
     1861 tatcaataat atcaataact gttccggttt taggtacggg ataaaggttt tccggagttc
     1921 tgcctacagg ccctagaggg cttagccgaa ttgttactcc ggactttgta gcgttttggg
     1981 gcagttctaa tgatcggtta tagtagctta cttgtacttc ggcgttgttt gtaaattttg
     2041 tgctgggaga gaaatcttcc ttccatagat acggactcac agttccttta tgtgctccca
     2101 gtacgtcttg ccaagaaaaa ttaggccacg gatcttgtat gtaatccgcc caccaagaaa
     2161 aaggatcaat aaagccgtcg ggaatatcgt ctgcaaaact gttgttatta tttttatctt
     2221 tgagtacggc taaatgcaaa tgtgggccgg tggtttttcc gctcagccct acgtaaccaa
     2281 taacttcgcc ctcatttaca tggatagggg agctggaact tgtaaacaga ccctcgcctt
     2341 gtaagtgcat gaagatcgtt tgatatccgt ttaagtggtc tatttttatt gtgtgcccac
     2401 agctgttgca gaaatagtag gtcgcattac cttcagcagg tgctttaatc ggtgttccaa
     2461 aagcaagttt aaaatcaatg ccgttgtgcc cggagtagta ctcaaagggc tgcttgtccc
     2521 ttatacccat aaagttcaga atcgtatctt gtctggataa cggctcctcg tagaaaccat
     2581 accccagaaa agggtattca tgatcaaaat acgatgttat aagatcaaga ggaatatctt
     2641 cggtattttg tatttcgaaa ggcagggata gaaatttact attatagtcg ttctgctcta
     2701 ttttatattt ttttaagccc tgttggtgtg tgtccctagc aggagctgtg gcgctgagta
     2761 cacgtgcgtt agtttatcaa cctttgttat agtattccct gaagtgctct cccagccttc
     2821 gtcaacacca cttttcttaa agacaaacgc gtttccgtag atatcgttta cagcaacgta
     2881 gagattctca tttattacaa tcatgtcttt gaccgcatat ccgtcaaaaa agctgtcgta
     2941 gctccacacg tctgtgcctg atactaagta tcgtagcccc aaatagtcat caccagcata
     3001 agtcctgttt tcatattctg cgaaactcga tattcttcta ttcctaaggc caattatctc
     3061 ttcccatgta tggccgttgt cggctgaaaa ataattattg ctggccatag aaatatagat
     3121 tttttctcct atcatgctga tggagaaaat tgtctttgtg ttagtttcgt ccggcattgg
     3181 tcgttcccag ttttgccctt tatcaaaaga aaaatatgcc ccgttgtacc tcttgcccac
     3241 aataattaga ttttcttttg cctttataat cccgcctgaa aaaggaaaaa cgtctgatag
     3301 atgttgccac gtgtacccgc cgtcagaaga tgcaaagatc ccgccggttc cgtcaatttc
     3361 gaacgcggta gtggcgtaaa ttgtgtttat tccatcgaaa gacattgtgg taacccctct
     3421 cccttcaagt ccgtttgttt ttatccatgt gtttcctagg tcgtgactta cgtaaattcc
     3481 attatatgga ttaagccata ttctatcgtc gaactctccc gctattaatc cgtagggggt
     3541 gttgataatg tttttgattt tgggatccag ctgttcccaa ctttcagacg caagcaaatt
     3601 attattaagg ctaagtagca taaaggctat aacacagatt gctatatttt tcattctatt
     3661 atagtaagaa aatattcgga aataccaact tgcattgtat attataagta gattttattt
     3721 taaaagtaat ttaccacaat agttgctaat attgataaaa agaggggcac tatttgattt
     3781 ctataataaa agattgatgc cgtctaggat aggccagtat tacttgggag taataaattt
     3841 actcacaatg aatagtttta gacatttggg cctatagata agagggatat ttaacttttt
     3901 ttcctcggtg ccgttcgtat catttttgct tttacttgtt ataaagttta taattcaagg
     3961 caacatgtta agtctatcca aaatttactc tagcaacccc gatctaagaa aagctcaagt
     4021 caaaggactt ctttttttat taaactccga agactctatt tctaataatg agctggttca
     4081 acgaaccggt ctttccaaag aagttttaaa aacatttaaa gaatcaattt cttcaatatt
     4141 aataaaaaca actgctgatg agctagcatt gacgaaagtc ggcagggagg ctgttgggga
     4201 gatgaatccg gaaccctatt cttggaccta cttaagcttt agctttgatg ctctaatgtt
     4261 gcaggcgctt gaagctataa aagctatacg ggaagggcag cagcaatttg tggcaaagag
     4321 agagtttgac cagttttttt gtactcctga aacttcgata cttaaggcaa aagcgattct
     4381 aaataaagtc ggtgggtctg aaggtgttag cattgctttg atgggggatg atgatttggt
     4441 ttcaatatca ttgggattgt tggaccgggc tgtagaagaa ataatcgttt ttgatgtaga
     4501 taaagatgtt ctgtctctaa taaaggaaaa ggctgaggaa ttaaaaatta cctccataaa
     4561 aaccgtttta tacgacgcaa gagatgatct tccaaaagac atggtcgcac gatttgacgc
     4621 agtgctgact gatccgccgt atacaagacc aggagtagcc gtgttcctag ataagtgcat
     4681 tacattatgc aaaaaagcta ctacatatgc ggggccatat atattttttt gttatggtaa
     4741 cggcctgcgc gaccctttaa aagggcttaa agttcaagat attattctta gcagaaactt
     4801 gttgatagag gaaaaaatat caacatttaa cagatatgac ggggcggaaa taattggaag
     4861 ctccagcgat ttgtttgtat tgaaggtttt agaaacaacg gaagttctgg aggattacac
     4921 cggtacagag atttacactt atgagaatac cgagatagag gatttcccat acgtagatca
     4981 ttatgttttt aagttatata aggttccttc gcacgttgtt acctctaaaa aaacattgca
     5041 gaagaatata ggcgtttttt gtaaatggca tggcttaaac gttgttgaca ctaaactaac
     5101 gagatttaag ggagggggat tcagcttcac gtacatactt tcaacttcaa atcttcttgt
     5161 ccatacgtgg cctgaaaaaa gggccctcca tatagatctc ataacctgct ctcccattaa
     5221 gaagaagcac ctgctcttaa agaatttatc tgatctgttt agtactatgt ccgtagaaat
     5281 cagaaaaatt gaatagaggt gtctcaagga gctcgaaaac aaccagcatt actatagctt
     5341 tattcacagt aaaaaatact caaaaaaaca gcgcgaacta tttgacttta taatggttag
     5401 ttggtatgtt tggttctgca catattgctt tagggtaatt atgtggtggt ttaagtagac
     5461 tcatgttagc ataagccaca caaatccaac ccgcacttta aaaaacaaat agttaaagtt
     5521 cgatgaattt aaggtgaaaa gaatgatttc ttttgagaat ttgatcctag ttcaggacga
     5581 acgctagcgg cgtgtttaac gtatgcaagt cgaacgaacc tcaacatctg ttttaaaccg
     5641 gcttgccggg gaaaaacttc gttgtggtta gtggcgaacg gctgagtaat acgtaggtat
     5701 ctacccctaa ctcagggata acttggggaa acccttgcta atatctgata gtctgtcagc
     5761 acttcggtgt ttgccagtaa agatttatcg gttagggacg agcctgcggc ctatcaggtt
     5821 gttggtgagg taatggctca ccaagcctat gacgggtacc tggtctgaga ggatgtacag
     5881 gcagaggggc actgagacac gggcctcact cctacgggag gcagcaaccg agaatcttgc
     5941 gcaatgggcg aaagcctgac gcagcgacgc cgcgtgaagg atgaaggcct tcgggttgta
     6001 aacttctgaa aatagcaccg gctaactacg tgccagcagc agcggtcata cgtagggtgc
     6061 aagcgttatc cggatttact gggcgtaaag agttctgtag gctgttttgt aagtcagtgg
     6121 tgaaatactc cagctcaact ggaggagcgc tattgaaact gcattactgg aggtagttag
     6181 gggatagcgg aactcatggt ggagcagtga aatgcgttga tatcatgggg aacaccaagg
     6241 gagaagtcag ctatctggga cttacctgac gctgagagac gaaagccagg ggagcgaaac
     6301 ggattagata cccgtgtagt cctggccgta aacgatgcct gctagatgcc tgtctttcgt
     6361 atgggtgtcg aagctaacgc gttaagcagg ccgcctgggg agtacggtcg caaggctaaa
     6421 actcaaagga attggcgggg acccgcacaa gcggtggagc acgctcttta attcgttgat
     6481 aaccgaaaaa ccttacccag gtttgacatg ctactgcaaa ctctgtgaaa gcagagctcc
     6541 ttcgagggtg tagcacaggt gttgcatggc tgtcgtcagc tcgtaccgtg aggcgtaccc
     6601 ttaagtgggt taacgagcgc aacccctacc gttagttgta tcactctagc ggaactgccg
     6661 gcgcaagccg gaggaaggtg gggatgatgt caagtcggta ttgcctttat acctggggct
     6721 agaagcatgc tacaatggcg cgtacaacgg gcagcaacac ggtgacgtga agctaatccc
     6781 ttaaagcgcg cctcagttcg gattgaagtc tgcaactcga cttcatgaag tcggaatcgc
     6841 tagtaatcgc aagtcagcta tattgcggtg aatacgttct cgggtcttgc acacactgcc
     6901 cgtcacgcca gcaaagctaa ggccacccga agtcccttgt attgagggcc caaggtggaa
     6961 ttagtgatga gggtgaaggc gtaacaaggt agccgtaggg gaatctgcgg ctggatcacc
     7021 tcctttctaa aggaaacaaa acgtgacgat agaaagcgta aacttgtcaa agatacatga
     7081 atttgtgttt gcgaaagtcc gctgtaatat cgcaccttct acatctgatt agttctagga
     7141 tgtagacagt aaaagtttgc acgtacctcg cagcaaacga gaacaggttt gaagatgagc
     7201 ctaaatcttc gagtgaatca ttctttttac cttatagata tcgaacttta aagaccatac
     7261 tacttttatt tacagagata gcggtgcgaa cctttttgta aaagcatcga aagaagcgat
     7321 ttaaaggaac atgcttttat aatctcgtta cttaaaacta cttgtgtaat ctggtaaatg
     7381 taatttaccc aacattttgg gatttgcagt gcaaagaaat taaagtaaac cacctgcgta
     7441 agctggcggt ggttgataat taacaaagtt tttttatggt gtatacttga agcatgcaag
     7501 gtggttttag agaaggtggt cggaacctaa taacagacaa agctgttgaa gaagctagtg
     7561 ttattgtcgg ttctctagag atgaaattaa gcaatcttca cgtaaaggct gaagttgaag
     7621 atgggggggg atattaatgc ccatgcagcc gttctcgacc aacttggcca atcggcggta
     7681 gcggaaaatc caacagcctt ggaagaagct gcagaaggtg aggcagcagg tcttgtaggt
     7741 caagcgcttg atggtatcgg tgtacatgaa attattgtta atctcgaaga aatagccgtc
     7801 ccaacaaccc ctgacacgta tatgtcagat gctgttagag agcaaagaat gaatgcggcg
     7861 gcgggcctag tcgtttctgc tctgactgaa caaggctggc ttgtacaccc taataacccc
     7921 ttgggcgtgt ctcagtccgg tcttcgagca aatgaagctt tatcagctga gctgggtctt
     7981 cggccaaggg ctccggatgc ggctcctgat tccctaaaaa gatttgtggt ctttttcaag
     8041 gtaagcctaa tttatgcgga gccagatgct cctagtgctt gattatctta attgaatacc
     8101 acaaagtctt tcttgtttac ttatggctgc atactttgta atgtttttcg taggcaaagc
     8161 tatttagttt tttgttttga aaaaatactc cctcattttt attgtttttc taagaccttc
     8221 gtcaagagtt gttagtggtg accatcctag attttttgaa gctttttcat acctaacgct
     8281 cagaaagcat tcctgtttcg gtctatcatt tgaagttggc ttttgctttt gaagccgctg
     8341 tccgggtttt ttattgtcga ttttcattag cttcgatata gtttcttgta actgcccgtt
     8401 gtttaattgc aggcctgccc cgatgttaaa aattccttcg cagtcttttt ctatagcttt
     8461 tacgtatgcg tcaatgtgat catcgataaa aataaaatct ctcacatcta gaggctgttt
     8521 aattttagga tcatttttat ttagtaatga gtcgatgatg tattctatta ggtaaaatcc
     8581 ggccccttct cgtccatagg tattggcagt tctcattata accattttga cgtcaggcat
     8641 cgcttgtgca aaatattcaa ttactctttc tgcgttttgt ttgcttagcc catatggtga
     8701 tgatgctggt cctagctcta gtgtttctga aaaaggttca tttgagttct gatagccgta
     8761 tacctccatc gagcccgcaa aaacgagctt tgctgtattt atgtcttgag agccctttag
     8821 gttttttatt gctgatagga gtttgaatgt tcctatctca ttagtttgta agtattcctc
     8881 aggaaaattg aacgaaagcc ctcctgacga tagcgctgct aaatgaacta taacttctgg
     8941 attcgcttct tttatggcgt tttccatgga tctttgatta ttgagatctg cgacaaaaag
     9001 ctttgcctgg gccgggactt taaggaggtc aatattgcta ttgatcctat gcatgccgct
     9061 tacttcatac ccttgttcta ttaatctttg tgctaatctt ttacctatga agccggtaat
     9121 gccggttata aaaatctttt tttcatattt tgctgatgtt taacggatgg tgggtctgga
     9181 tggactcgaa ccatcgacct ttcccttatc agaggaacgc tctaaccgcc tgagctacag
     9241 acccccaaac ctttcatgat ttgaaagtat tggcctatca tagcaaggtt taaaatattt
     9301 ttcaaacttg aggtttttaa tttttttcct ttgcaacgta aagaaattac agcaaaccgc
     9361 ctgcgtaggc tggtggcggt tcatagttga cacgcctcag ccaacgtgtt aacttactct
     9421 caccaaatac tccaacttct accttagggt ttaaagtttg gtaggttaca gtttttgtaa
     9481 tctccgacgg aagctctgat tccaaataat attaatagtt tatgtgtatg gatctgaaac
     9541 gggttcatat gataatatgt ttttgctttc gcacaaacgt gtttgcgtat gggcctgtag
     9601 cttagttggc agagcgctac atttgcaatg tagaggtcac gggttcgact cccgtcaggt
     9661 ccaccaaact atatagataa tctttatcta ggtactttga aaactgaacg aaaaacaatg
     9721 ttagttcaac atgagaatca agtactttgt tgtcgcaaga cagcagggta gtttaattct
     9781 tgaagttccg tgtttacacg gaacacaacg tcttagtatt agaaatataa aaagttacta
     9841 atgcatacgg tggatgcctt gggtctttgt gacgatgaag gacgtgctag actgcgttaa
     9901 gcctcggtga gctgtcaaga agcgttatca gccggggatt tccgaatggg gaaacctaat
     9961 ccatggcaac atgagattgt tctttactga aaatattgct tttagcaata tataggtact
    10021 ggacggtaac ccagcgaact gaaacaacat agtagctgga ggaatagaaa ctaaataagt
    10081 atttccttag tagcggcgag cgaaaaggaa gaagcccaaa cccggactga attagaaaaa
    10141 agagtttttc ttgtgtaggt gaagcaattt tttgctttgt ttatgcagga aggctctgct
    10201 ttcttattca gtataaaggg ggtgtggaca accctgtaat ttaatttaca agaagtaaaa
    10261 aagtattttt gtaggagaag ctgcctggaa aggcacgcca cagaaggtta tagccctgta
    10321 tccgaaactt aaataccttc tgggttgctc ccgagtagca tgggacacga gaaatcctgt
    10381 gtgaatatag ctggaccatc agctaaggct aaatacacaa agacaccgat agtgtacata
    10441 gtaccgtgag ggaaaggtga aaagaacccc tgttagggga gtgaaataga acctgaaacc
    10501 gtatgcttac aagctgacag agctcttttt tgagtgatgt tgtgcctatt gaagaatgag
    10561 ccggcgagtt aatgttgcgt gcaagcttaa acgactaagt cgtgtaggca aagggaaacc
    10621 aagtcttaat agggcgccta gtacgtaaca ttagacccga agccaagtga gctaaccatg
    10681 agcagggtga agcttgtaga aatacaagtg gaggcccgaa ccggtgtctg cggcaaaagg
    10741 ctcggatgac ttgtggttag aggtgaaacg cttaacgaac ttggctatag ctggttctcc
    10801 ccgaaatata tttaggtata gcctcaactt taaatgactt gtgaggtaga gatactggaa
    10861 agaactgggg cgcgaaagtg tacccattct taccaaactc cgaatgcgca agttttagtt
    10921 gggagtcagc actggggagc aagttcctgg tgcgagaggg aaacagccca gaccgtcaga
    10981 taaggtccct aaactttgct tagtgggaaa ggaagtagaa ctgtttagac agctaggagg
    11041 ttggcttaga agcagccacc ctttaaagat agcgtaatag ctcactagtt caacggtttt
    11101 gcgccgatga tgtaacgggg ctcaagcaaa gtaccgaagc tgcggatccg cttttgcgga
    11161 tggtagggga gcattgatgt cagctgcgaa ggttgatcgt gaggttggct ggagcggcat
    11221 caagtgagaa tgtcggcatg agtaacgaga gggtggtgaa aacccacccc gccggaagcc
    11281 taaggtttcc tccgcaacgt cagtcaacgg agggttagtc gggcctaagg tgaggccgaa
    11341 aggcgtaatc gatggacagc aggttaatat tcctgcactg ccataaaatc gctattactg
    11401 atggagggac ggagtttgat agatcgagcc cattatggat ttgggtccgg aagacaagac
    11461 agtctgtgta ggcaaatccg cacaggcgtt tattcgagtc ggcgggaagg cccgtaaaaa
    11521 ggtcaattcg gttgcgttaa gcttccaaga aaagcttcta aggagatttt atggtatccg
    11581 taccgcaaac cgccacaggt aggcaggtag agaatactaa ggcgaacgag agaaccgtgg
    11641 tcgaggaact aggcaaatta catccgtaac tttgggaaaa ggatgcccta cttgtcattg
    11701 atgggtaggg ggcacaaaaa tggctcaagc gactgtttag caaaaacaca ggtctctgct
    11761 taactcgtaa gaggaggtat aggggccgac gcctgcccag tgcccgtaag ttaaagccgg
    11821 gggtgatcat cgtttaggcg atgtgagctt ttggtcgaag ctcgggtgaa cggcagcagt
    11881 aactataact gttctatggt agcgtaatcc cttgccgggt aagttccggc ccgcacgaaa
    11941 ggcgtaacga cttgagcact ctcttagtca cggactcggt gaaattgaac taccggtgaa
    12001 gatgccggtt actcgttgtt ggacagaaag accccgtgga gctttactgt agtctggtat
    12061 tgataagcga tactaactgt gtagcgtagg agggagtctg aggcgtaaaa cccgaagaca
    12121 tcaatgaaac acctctcttt tattattgtg agtctaacta tgcaccgtga atccggtgcg
    12181 aggacaatgc ttgatgggca gtttaactgg ggcggtttcc tgctaaaaag taacgcagga
    12241 gcccaaaggt tcccttatcc cggatggaaa tcgggatgta agcgtaaagg tatatgggag
    12301 cttgactgca agacctacaa gtcgagcagg tacgaaagta gggcttagtg atcttccaag
    12361 tgcttaatgg taggcccgga acttaacgga aaaaagttac cccggggata acaggc
//
LOCUS       DAOSWN010000017        12335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_3255_length_12335_cov_5.074756, whole genome shotgun sequence.
ACCESSION   DAOSWN010000017 DAOSWN010000000
VERSION     DAOSWN010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12335
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3255_length_12335_cov_5.074756"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1071..2090
                     /locus_tag="PLF29_01480"
     CDS             1071..2090
                     /locus_tag="PLF29_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HXK52640.1"
                     /translation="MNNFKHYVSNYLEYCEIEKNLSQNTIKMYDFYLNDYLTWAQGYL
                     GKDEIHIEDITYEVVKKYRLFLNRRKSTKSHEEFKKSTQKTFLVALRSFLKYLIVEEN
                     LAVLSPDQIILGKPEPSIPKVLDETQLVRLLDVQNLSKKSGIRDRAILETLFSTGLRV
                     GELVALNRDEVNTDSGEFTVIGKGKKVRTVYLSENAKVWIRRYLDTRRDDYAPLFLRY
                     SGKKMEPSDIEGESLRLTARSVQRLVKKYVLRSGLAVDATPHTLRHTFATGLLQEGAD
                     LRSVQELLGHSNVSTTQIYTHVTNRQLKEAHKKYHKDITTTADSKNDYVENDPRRIKS
                     EEEIE"
     gene            complement(2128..3786)
                     /locus_tag="PLF29_01485"
     CDS             complement(2128..3786)
                     /locus_tag="PLF29_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52641.1"
                     /translation="MFNSFNSFGNKGYGQSGSEFGYSENRRGNFKFTLENPLATGDES
                     LSEIEAELSPLLTENYNCVPCDFETEYDFFSRGKNTAEILKRILMFAFSSRIKVSIDV
                     MPHIQERGRTTDPTTGSTKTNKMFYTDLVEGVGSKHRVQQGIFYVHGDKKLHKLTPKN
                     YRKGTSMYEGSLHPFDIPRNRKLDERYKLNPKFCEYRPVAIEGEPDFVVIQTTVAHRE
                     TNKVAYEYWDILVREELYEPDLHAKQAANIVSFPTDTNLIFSSMHPVAIQAFNVFVAK
                     VVASPNNYYDKEELLSSMGNLQNVFDIVNIHIIKNKKMVSVETCKLNQVVTFGTKTTT
                     NVSQKQGIQPLQQPANNGDPFATSNQGFNNGFDDPYLYDDFNNNYPSYGTNNGLNSQG
                     NYGLNANDPYNNYGYGAYPNGNSNYGAAPQDQNQNTDSLKDHDMTRDDSTLKKESRVV
                     EISDTDGLKILTRKRLEDKSVKETRSGLGISTQINAEYSFSNFLIDSAKFILVDTTES
                     KFDYMNANEKSSRRERSILINADKLENQEKAALDDLLVEHSTQA"
     gene            complement(3876..4610)
                     /locus_tag="PLF29_01490"
     CDS             complement(3876..4610)
                     /locus_tag="PLF29_01490"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HXK52642.1"
                     /translation="MTHKKIFTTIESLKKRLVYEKELYNKGLRHIVGIDEVGRGPLAG
                     PMVFSAVALDLQQIFNSINKIQDLAHKTDENINNDVTLFLEQTAQINDSKKLSAKKRE
                     ESAVFIKQSALKYSIFEIDNHTLDNIGLAKATQAGFFSALKNLNIDVEHVLTDQYKIN
                     AFNSSKQTNVTRGDQLSLTIGAASILAKTYRDGLMLKIHDEFPLYGFDKNKGYGTKLH
                     IEQIRLLGPCEHHRATFEPLKSFIEG"
     gene            complement(4745..7384)
                     /locus_tag="PLF29_01495"
     CDS             complement(4745..7384)
                     /locus_tag="PLF29_01495"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HXK52643.1"
                     /translation="MADEEIKTIAENSDDDVLNEQLDPTIIKADISKEMQKAYLDYAM
                     SVIVSRALPDVRDGLKPVQRRIIYAMQDQNMQVSGKFHKCAAVVGEVMKKYHPHGDAS
                     IYDALVRMGQDFTLRYPLIFPQGNFGSIDGDSPAAFRYTECKLQKIAEELYKDIDKET
                     VNHVVNDLQVEEPVYLPSVLPNLLLNGVTGIAVGMATNIPPHNLGEIIDGLELLINKA
                     TNIGPEPTDEDIIDVIDSKEPDSPALLNYRLKVTKTDFVSAATVEDLTQFIKGPDFPT
                     GGTIYDQKEILQMYATGRGRVVTRAKMEIEETKAGRTRIIVTEIPYQVIKATLVSKIA
                     ALVHEKKIKDIAELRDESNREGMRIVIELKKDAIVKKVQNQLYKYTPLQNTFNTNMVA
                     LLNGEPKLMTLKIILEEFLKHRQEIVVKRTLFLLKKVKSREHVLQGLKIALDNLDEVI
                     KLIRASKDAETAKTGLMSKFALSHIQAQAILDMQLRKLAALERKKIEDELAEIVNTIE
                     GFEALLGSPQLIINEVKNELLEIKQKYADERHTKVVKGKIGEIADEDLIPNEQCIITI
                     SENGYIKRLNENIYKKQGRGGKGVKGQELKEEDVVNTIRLCETHDYALFFTNTGKVYK
                     LRIWEIPETGKSSKGTALVNFLNIRQSEYVQSMLTLTPAQMEAEQGFIFFGTELGRVK
                     KTSMNDFANIRTSGIIAINLTEGDNLAWVRITKGQDDIILTTKMGMSIRFNENDVRQM
                     GRAAAGVTGIKFKKKGDSVIGMVRVPQDKEQKDFKLLVVSSKGMGKKTDLSEYKKQNR
                     GGSGILTYKVSDKTGDLIAARMINTTVENDIIIATRTGKFIRLDEEQLPTLGRATQGV
                     KLIRLNDGDGVTSVAVVSENEEE"
     gene            7665..9014
                     /locus_tag="PLF29_01500"
     CDS             7665..9014
                     /locus_tag="PLF29_01500"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HXK52644.1"
                     /translation="MQKLIDTVKKLHDKEYRKAGHSVFDHVLAVYNYLLDLDIDDRVM
                     LQAALIHHSLPEHEAAVTKVCDAETIAFAKNYQQVRQLRINVKPYFDADRIVAAINTF
                     EDTRLLVLRLVDKAVDSGSLFALLPEDRKKQAIKRIYYYAPLANLLGLSKVQRVLEEN
                     SFKTLYPEKYYEVEDFVAEKAGGLGLVLKDAISFIDSILYENSIPATVEGRIKTIPSL
                     FKKAWSKTNNGPQDVADLLAIRVITDSVANCYAAESLLNNIWHASESLRNDYIQNPKP
                     SGYRSLHLTYRMSEELMIEVQFKTAEMHEYNEHGAASHIIYKFGPSFKQVLLENSGFL
                     KRLNFLENSENSNIKIPTKNIFAYTPKGDVVKLPAGSTVVDFAFSLHSDLGLACNGAL
                     VNNSIRPLKYELQTGDTVTILLKKSHNPVSRDWLQIAKTKRARSAIRKAIRKSSETQ"
     gene            complement(9153..10103)
                     /locus_tag="PLF29_01505"
     CDS             complement(9153..10103)
                     /locus_tag="PLF29_01505"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HXK52645.1"
                     /translation="MRIFVIGHKSPDLDSVAAPVAYAEYLRLIGRYEDAELIAVSAEK
                     ANAETEFVFNKFEMPLPKTIEDFEIDASDSFVLVDHSEDSQRSEKVIVENIIEIIDHH
                     KTNVNVPHPIRIDIKPLGSTATLVYEYFKFNSIKPAKGIMGILLSSILSDTQGLKSST
                     TTGEDYLYAHDMARALGLIISDLTFEIFKAKSNIAGLSVEDLVVKDYKIFDFGGKNVL
                     INQIETVEPEIVIEMKDKILPALKNLKQKLDIDMAFFVITDILQINSKIICETKKEQA
                     VAEKAFTASFENDIADIGPKMSRKKDIAPALEKTINNQKK"
     gene            complement(10177..12180)
                     /locus_tag="PLF29_01510"
     CDS             complement(10177..12180)
                     /locus_tag="PLF29_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HXK52646.1"
                     /translation="MAKEEKTKKSKADLDNNYGSDQIQVLEGLDPVRKRPGMYIGSTG
                     QQGLHHLVTEVVNNSMDEAMAGYANYIKVEFFKDGSVAIYDNGRGIPYDEKKGYGVSA
                     LELAFTKLHAGGKFGAGGYKVSSGLHGVGASVVNALSEWCRVIVKRGDELVIQEYADG
                     GKVLRKVEPLDPKNPISKVKNAEWSLKLEEWDAESGTIVQFKPDETIFETVDFKYKFF
                     VNQLREYAYLTANIKFDIIDHRTDQRYCFYFEGGVKAYIKSLNRSRKPMHPEIFHIHK
                     NYENIDVEIAFQYNNSYAENVLTFANHLRTGEGGTHLTGFRSALTKTINDYAKKHNLI
                     KDKAEALSGDDLKEGITAIVSVNIDSAQLQYEGQTKGKLGTSEARTAVETVTKEALEV
                     YLEENPKDAQAIIDKNLLALKARIAAKAARDTVIRKGALDTGGVLPGKLSDCSEKNSE
                     KTELFIVEGDSAAGPAKQGRNRMYQAVLPVFGKPLNTERARLDKIVKSDKFKPLIIAI
                     GTGIGEQFDVSRLRYGKLIIMSDADVDGAHIVTLHLTFFFRHMPQLIENGNLYVAVPP
                     LFKAVYGKNKQYITDERELEAFLKTDIGKKSLIQRFKGLGEMNDNELWDTTMNPETRR
                     LKLITLGDAAHADEVFSNLMGEEVAPRKRFIQTHAKNADVDVT"
BASE COUNT         3220 a         2359 c         2381 g         4375 t
ORIGIN      
        1 tagcatctgc taacattttt caaaaacccc aaataactat agggttatat attttaataa
       61 aaaacacaaa ataatgaaga aagttttata taaaagttgt ttttagcttg ttgttgagct
      121 ttaaaatgta ttttacagtt gaaaatagta aaagatcttt accccaaaga gaagagggag
      181 ggggacgatt gtcatactta ccctatagta tgtttgtcat acttttcttt ttgtacgttt
      241 gtcatacttt tttaaagcgg tgcaattatt gtattgtgga taattcgggt ggtcgtttgt
      301 agtttttgtt tgagggcctg ttttaaaaaa tataaatgaa taataaatag atctatttat
      361 atataaaatg gtcaattatt gttttcaata ttacttttag atctttacac tccccattta
      421 tcgtttgtct gggtgcgtta atagtgcttt ttacttaaac aaggtttaac tagataccct
      481 acgtcgttaa acatacatta tacgacgtta ctcaaaggtc tcaacttgtg ccctttctta
      541 gctttttaac ggtgttattt ctactaaatt catttttaaa tttttgcatt aataaatacc
      601 atcgtaactt tcctattaca atttcctttt taatttttat attttatttt aaattacgtt
      661 atacttattt atattatttt ccccgctttt ttgccgcctt tgttttatct accccctctt
      721 cctagtttgc ttgctgtttt cttacttttc gcgtaaaagt atatctttat ttctgcaacg
      781 ggaagacccc gtgcttatct cgtggatgaa agcgcgagct ttcaaccgca aaaaagaaga
      841 gtccggagac tttagttcgc ggagtcttta ttcttggctt tgttaaggcc taaaatggct
      901 gtttgggcct attttgactt ctaaggcatt tttccaaaaa agttgagttg catatctttg
      961 aatctggctt ccttgaggat tcgggccagg gagatggtgc tcattgcaat attgttcgct
     1021 cttagttaag tatatttctg ctaatgtcgt taaagtgtta aaattagata atgaataatt
     1081 tcaaacatta tgtgtcgaac tatttagagt actgtgaaat agaaaaaaat ctttctcaga
     1141 acactattaa aatgtacgac ttttatttaa atgattattt aacatgggcc caaggatatt
     1201 taggtaagga tgaaattcat atagaagaca ttacatacga agttgtaaag aaatacaggc
     1261 tttttttaaa tagaagaaaa agcacgaaat cgcatgaaga attcaagaaa tcgacccaaa
     1321 agactttttt ggttgctcta aggtccttcc ttaagtattt aatagttgag gaaaatttgg
     1381 ctgttttgtc gccggatcaa ataattttag gtaagccgga accctcgatt cctaaagttt
     1441 tggatgagac tcagttagtg aggttgctgg acgtgcaaaa tctaagtaag aagtcgggaa
     1501 ttagagacag ggctatactt gagactcttt ttagcacagg tttgcgggtg ggtgagcttg
     1561 tggcgttgaa cagagacgaa gttaacacgg attcaggaga atttacggtc attggtaaag
     1621 gcaagaaggt tcgaacagtt tatctaagcg agaatgctaa agtttggata cgtcgttatt
     1681 tagatacgcg acgcgatgac tacgctcccc tttttttgcg ttattccgga aaaaaaatgg
     1741 aaccgtccga tattgaaggc gaatctttaa gattaactgc cagaagtgta cagcgtttag
     1801 ttaaaaaata tgttttacgt tcgggcttag cagttgatgc aacccctcat actttgaggc
     1861 acacttttgc caccggccta ttgcaggaag gcgccgacct tagaagtgtt caggagttac
     1921 ttggtcatag taatgtttct acaactcaaa tctataccca tgtaacaaat cgccagctta
     1981 aagaagcgca taaaaagtac cataaagata ttacaacaac tgcagattct aaaaatgatt
     2041 atgttgaaaa tgatcctcgt cgaataaaat cggaagaaga gatagagtaa atattgtacc
     2101 cacgatattt agtcttttgg cacatttcta tgcttgagta gagtgttcga caagcaaatc
     2161 atccaaggct gctttttctt gattttcgag tttgtcggca tttatcaaga tgctgcgctc
     2221 gcgtcggcta gatttttcat tggcgttcat atagtcaaat ttgctttcag tcgtatctac
     2281 aagtataaat ttagctgagt ctattaagaa gtttgagaaa gaatactcag catttatttg
     2341 ggtgcttatt cctagtcctg atcttgtttc tttaacgctt ttatcttcaa gtcttttgcg
     2401 agtaagtatt tttaagccat cggtgtcgga gatttctacc accctagact ctttttttaa
     2461 tgttgaatca tctctagtca tgtcatgatc ttttagcgaa tctgtatttt gattttggtc
     2521 ttggggtgca gctccataat tgctgtttcc attaggatag gccccgtagc cgtagttgtt
     2581 atagggatcg ttggcattta gtccataatt tccttgcgaa tttaagccat tattagttcc
     2641 atacgaaggg tagttattgt tgaagtcgtc gtagaggtaa gggtcgtcaa aaccattatt
     2701 aaagccctgg tttgaagttg caaaaggatc tccgttgttt gccggttgtt gtaacggttg
     2761 tatgccctgt ttctgagata cgtttgttgt tgtttttgtt ccaaaggtta ctacttggtt
     2821 aagtttgcaa gtttcaacgg ataccatttt tttatttttg atgatgtgta tatttactat
     2881 gtcaaataca ttttgcaggt ttcccattga agatagaagt tcttccttgt cgtagtaatt
     2941 atttggcgag gccacaactt ttgcaacaaa aacgttaaag gcttgtatag ctacagggtg
     3001 cattgagctg aatattaagt tggtgtcggt cggaaagctt acgatgtttg cagcctgttt
     3061 ggcatgtaaa tctggttcgt aaagctcttc tcttacgaga atgtcccaat actcataagc
     3121 tactttattt gtttcacggt gtgcaactgt ggtttgaata acaacaaagt ccggctcacc
     3181 ttcaattgcc accggtctgt attcgcagaa ctttggatta agtttgtagc gctcgtctag
     3241 cttcctgttt ctgggaatat cgaacgggtg taatgagcct tcgtacatag aagttccttt
     3301 tctatagttc ttcggagtta gtttgtgcag ttttttatct ccgtgaacat aaaatatacc
     3361 ttgttgaact ctatgcttac tcccaacccc ctccacaaga tccgtataga acattttgtt
     3421 tgttttagta cttccggtag taggatctgt agtgcggccc cgctcctgta tatgtggcat
     3481 tacatctatt gaaaccttga ttctggatga aaaagcgaac atgaggattc tctttaatat
     3541 ttcagctgta ttctttcctc tagagaaaaa gtcatactct gtttcgaagt cacatggtac
     3601 gcagttgtaa ttttcagtta ataacggaga taactctgct tcaatttcag aaagagattc
     3661 gtccccggtt gctaaaggat tctccaaggt aaacttgaag ttcccacgtc tgttttcaga
     3721 atatccgaac tctgatcctg attgtccgta acctttattt ccaaagctat taaaagaatt
     3781 aaacatatat tgacctcgtt ttttaagata agcggatttt accacaaatt actataaggt
     3841 gcaatttatc tatcaacttg actttgaagg ccgctttagc cttctataaa ggactttaat
     3901 ggctcaaagg ttgcgcggtg atgctcgcat ggacctaaga gtctaatttg ttctatatgc
     3961 agtttagtgc cgtaaccttt gtttttgtca aagccgtata ggggaaattc atcgtgtatc
     4021 ttaagcatca agccatccct ataggtcttg gctagaattg atgctgcccc gatcgtaagg
     4081 cttagttggt cccctctcgt aacatttgtc tgcttgctcg aattgaaagc gtttatcttg
     4141 tattggtctg ttaatacatg ttcaacatct atatttagat tttttaatgc actgaagaac
     4201 ccggcttgtg ttgcttttgc cagtcctatg ttgtctagtg tgtggttgtc tatctcgaaa
     4261 atgctgtatt taagagcact ttgcttaata aaaacagcac tttcctccct ttttttagca
     4321 gataacttct tggaatcgtt gatttgcgct gtttgttcca ggaatagtgt aacgtcgtta
     4381 tttatgtttt cgtcggtttt gtgagccaga tcctggattt tattgatgct gttgaatatt
     4441 tgttgtaaat ctagcgcaac tgcagaaaaa accatcggtc ctgctaaagg tccccgcccg
     4501 acttcgtcta ttcctacgat atgacgcaaa cccttgttat acagctcttt ttcataaaca
     4561 agcctttttt tgagagattc gatggttgta aatatttttt tatgcgtcat ttataattta
     4621 tttattgaga taatcctgcg aactcaattt ttagataaaa gcttaggtac aggcatcaaa
     4681 gaaggtccct tgaatttgac tagtcgtgcc ttattaatgt tgttatttga agatgaaaag
     4741 agggctattc ttcctcattt tcacttacta ctgcaacaga tgttacaccg tccccgtcgt
     4801 ttaggcgaat aagctttacc ccttgtgtgg ctcgccccag agttgggagt tgttcctcgt
     4861 caagcctaat aaatttacct gttctggttg caattataat gtcgttttcc actgttgtat
     4921 ttatcattct ggcagcaatt aggtcgccgg ttttatctga aaccttgtaa gttaatattc
     4981 ctgagcctcc tctgttctgt tttttatatt cgctgagatc ggttttttta cccattcctt
     5041 tagagctaac tactagaagc ttaaagtctt tttgttcttt atcttgaggg acacgaacca
     5101 tgcctattac ggagtctcct tttttcttga acttaatgcc tgtgactcct gctgctgctc
     5161 ttcccatttg cctgacatcg ttttcattga agcgaatcga cattcccatt ttagttgtaa
     5221 gtatgatatc gtcttggccc tttgttattc tcacccatgc cagattatcc ccctcggtta
     5281 agttaatggc tataattccc gaagttcgaa tatttgcgaa gtcgttcatt gaggtttttt
     5341 taactctacc aagctcggta ccgaagaata taaagccttg ttcggcttcc atttgtgcgg
     5401 gggttaaggt tagcatggac tgtacatact cactttgccg aatgtttaaa aagttaacaa
     5461 gtgctgttcc tttcgaagac tttcctgttt ccggaatctc ccagattcta agtttatata
     5521 cttttcctgt gttggtaaaa aacaaagcgt aatcgtgagt ttcgcataag cgaatagtgt
     5581 taacaacatc ttcctctttc agttcctggc cttttacacc ctttcctccc ctaccctgtt
     5641 tcttatagat attttcgttt agccttttta tgtaaccatt ctcagatatt gttataatgc
     5701 attgctcgtt agggattaaa tcttcatctg caatttctcc tatttttcct ttaacaactt
     5761 ttgtgtgcct ttcgtctgcg tatttttgct ttatctctaa gagttcgttt tttacctcgt
     5821 taattattag ttgaggtgag cccaacaaag cctcaaatcc ttcaatggtg tttacaattt
     5881 ctgcgagctc gtcttcaatt tttttgcgct ctaatgcggc caactttctt agctgcatgt
     5941 caagaatggc ttgtgcctgt atgtggctca aagcaaactt tgacataagg cctgtttttg
     6001 cagtttccgc gtctttgctg gccctgatta attttataac ctcgtccaga ttatcaaggg
     6061 ctatttttaa accttgtagc acatgctcgc gggatttaac ctttttgagt agaaatagtg
     6121 tccttttaac tacaatttct tgtcggtgct ttaaaaactc ctctaaaatt atttttaggg
     6181 tcattagttt aggttctccg tttagtagag caaccatgtt tgtgttaaat gtgttttgta
     6241 aaggggtgta cttatagagc tggttttgta cttttttaac aatggcgtct tttttaagtt
     6301 ctataacaat cctcatgccc tctctgtttg attcatctcg tagttctgcg atatctttta
     6361 ttttcttttc gtgtacaagg gccgcaattt tggatactag ggtggctttt attacttggt
     6421 agggtatttc ggtaacaatt attcttgttc tcccggcttt cgtttcttca atttccattt
     6481 ttgctcttgt tacaaccctt cccctacctg tagcatacat ttgaagaatt tctttctgat
     6541 cgtatatggt gccgcctgtt ggaaaatcag gcccttttat aaattgcgta aggtcctcga
     6601 ctgttgctgc ggaaacgaag tcggttttag tgacttttag tcggtaattg agcagggccg
     6661 ggctgtcggg ttcttttgag tcaataacat cgattatgtc ttcgtcggtg ggttcaggcc
     6721 ctatgttcgt tgctttatta ataagtaatt caagcccatc aataatttcc cctaagttat
     6781 gaggagggat attagtggcc atgcctacag caattcctgt taccccgttt aataaaagat
     6841 ttggtagtac agatggtaaa tagaccggtt cttctacctg taagtcatta actacatgat
     6901 tcacagtttc tttatctatg tctttatata gttcttcagc tattttttgc agtttacatt
     6961 ctgtatacct aaatgctgcg gggctgtctc cgtctatgct tccgaaattt ccttgcggga
     7021 aaattagtgg gtacctaagg gtaaagtctt gtcccattct gacaagtgca tcgtagattg
     7081 aggcgtcgcc gtgagggtga tactttttca taacttcgcc tactactgct gcgcatttgt
     7141 gaaactttcc actcacttgc atgttttggt cctgcatcgc ataaattatg cgtctttgta
     7201 ccggtttaag accatctcta acgtccggaa gtgctcttga aacaattacg ctcatggcat
     7261 agtctaagta agctttttgc atttctttgg agatgtctgc tttaattatt gttggatcca
     7321 actgttcgtt taatacgtca tcgtcactgt tttctgcgat tgtttttatt tcttcgtctg
     7381 ccatagccta gtcatgatag cagttttttt tatatgtatc aatttataat ttcgttactt
     7441 aaaactactt gcataagcta gtagatgtag tttacccaac atgttaggct ttgtaatgca
     7501 aagaaattac agcaaaccac ctgcataagc cggcggtagt tgattaagtg cttctttttg
     7561 tttaaaaaag tagcagctta gattattttc tgtaagaaac cgatagactg gttgtagcaa
     7621 tagacgtctc ttgtaaattc ccgaacataa actagaatat agctgtgcaa aagcttatag
     7681 atacagtaaa aaaacttcat gataaagaat atcgaaaagc cggacattcg gtttttgacc
     7741 atgtactggc agtttataac tatctgttgg atttagatat tgacgatcgg gttatgctcc
     7801 aggctgcttt gatccaccat tccttgccgg aacacgaggc cgcagttacg aaagtgtgtg
     7861 acgcggaaac tatagctttt gctaaaaatt accaacaggt aaggcagctg cgtattaatg
     7921 taaagcctta tttcgatgca gatagaatag ttgctgcaat taataccttt gaagatactc
     7981 gtttgttggt tttgcgattg gtagataagg cagtagattc aggtagttta tttgctctac
     8041 tgccggaaga tagaaaaaaa caagcaatta aaagaatata ctactatgcg ccgctcgcaa
     8101 atcttcttgg tttatctaaa gttcagaggg ttttggaaga aaattccttt aaaactctgt
     8161 atccggagaa gtactatgag gtggaagatt ttgttgccga gaaggctgga ggtttgggct
     8221 tagtgttaaa agatgcaata agttttatcg actctatatt atacgaaaac tctattcccg
     8281 ctacagttga gggaagaatt aaaactatcc cctctttgtt taaaaaagct tggtcaaaga
     8341 caaacaatgg tcctcaggat gtagctgatc ttttagcaat acgagttata accgattctg
     8401 ttgccaattg ttatgcggcg gaaagtttat taaacaacat atggcatgcc tccgaatctt
     8461 tgcgtaatga ctacatacaa aacccaaaac ctagcggata tagatccttg catttaacat
     8521 accgtatgtc ggaagagctt atgattgagg ttcagttcaa aactgccgaa atgcatgaat
     8581 ataacgagca cggagctgca agtcatataa tttataaatt cggaccatct tttaagcaag
     8641 tgttgctaga aaattctggg tttttgaaga ggttaaattt tttagaaaac tcagaaaatt
     8701 caaatattaa aattcctacc aaaaatatat ttgcatatac tccaaaagga gatgttgtaa
     8761 agctacctgc cggttctaca gtagtggatt ttgccttttc cttgcactcc gatttaggtc
     8821 tagcttgtaa tggggccttg gtaaataatt ccattagacc tttgaaatat gaactgcaga
     8881 cgggagatac cgtaactata ttgttgaaaa agtcacacaa ccccgtttcc cgagactggc
     8941 tgcagattgc aaaaaccaaa cgagcaagat ctgcaataag aaaagcaata agaaagtctt
     9001 ccgagaccca atagagcata cgatgcccat gctgtttctg tttttaaagt cgccgaattg
     9061 tgtcagtaag cggttcggta ggtatagaga gacacacatt ggtttgtcat aagtagcgac
     9121 agaaagtaat tgtgtcgcaa aactgcggaa tttcactttt tttgattgtt gatggttttt
     9181 tccagtgcag gagcaatatc tttttttctg ctcattttcg gtcctatgtc ggctatgtcg
     9241 ttttcgaaag aagctgtaaa tgctttttct gccactgcct gctctttttt tgtttcgcag
     9301 attatcttag aatttatctg taaaatatcg gtaatgacga aaaaagccat atctatgtcc
     9361 agtttttgct taagattctt caaggcagga agtattttat ctttcatttc tattacgatt
     9421 tcaggctcaa ctgtttctat ctgatttatg agaacatttt tacctccaaa atcgaatatt
     9481 ttgtaatcct taactaccag atcttcaaca gaaagaccgg ctatattgga tttagcctta
     9541 aagatttcga atgtaaggtc ggagattatt agtcctagcg cacgggccat atcgtgtgcg
     9601 tataaataat cttcccctgt tgtagtgctg gatttcaacc cctgtgtatc agaaaggata
     9661 gatgaaagaa gtattcccat tatccccttg gcaggtttaa tggagttgaa tttaaaatat
     9721 tcataaacaa gcgtagctgt agaccccaga ggcttgatgt caattcttat tggatgcggc
     9781 acattaacat ttgttttatg gtggtcgata atctcaataa tattttctac tataaccttt
     9841 tcgcttcttt gagaatcctc cgagtgatcg actagcacga aagaatccga agcatctatt
     9901 tcaaaatctt ctatagtctt tgggagaggc atttcgaatt tgttgaagac aaattctgtt
     9961 tcagcatttg ccttttcggc cgataccgca atcagttccg cgtcctcgta ccttcctatg
    10021 agtcgaagat attcagcata tgctaccgga gctgcgacag aatcaagatc cggagattta
    10081 tgtccgatta caaaaattct cattgatctt catttatacc acttaagata gtaggtttca
    10141 aattgtgttt aggtttaggg cttggatttg aaggttttat gttacgtcga cgtcagcatt
    10201 ttttgcatga gtttgaataa atctttttct cggagctacc tcttctccca tcagattgct
    10261 gaatacctcg tcagcgtgtg cggcgtcccc tagtgttata agctttagtc ttctagtttc
    10321 agggttcatt gttgtatccc aaagttcgtt gtcattcatt tctcccaaac ccttgaacct
    10381 ttggatcagg gatttttttc caatgtcagt ttttaaaaaa gcctcaagct ctcgctcatc
    10441 tgtaatatat tgtttgtttt tcccatacac ggctttaaat aaaggtggca cggctacata
    10501 caagttgccg ttttctataa gctgcggcat gtgtctgaag aagaacgtaa ggtgtagtgt
    10561 gacaatgtgc gcgccgtcta cgtcagcatc tgacataata atcaacttac cgtatcttag
    10621 tctcgacaca tcaaattgct ctccaatacc ggttcctatc gcgattatta aaggcttaaa
    10681 tttatctgac ttaactattt tatccaatcg cgccctttcg gtatttagag gttttccaaa
    10741 gaccggcaat actgcctgat acattctatt tcttccttgc ttggcgggac ctgcagcaga
    10801 atctccttct acaataaaaa gctcggtttt ttccgagttt ttttctgagc agtcggatag
    10861 ttttcccggg agtactcctc ccgtatctaa tgcgcccttg cgaattacgg tgtctctggc
    10921 tgcttttgca gcaattcgtg ctttgagagc caaaaggttc ttgtcaatta ttgcttgtgc
    10981 gtcttttgga ttttcttcca gataaacttc gagtgcctct tttgtcactg tttctactgc
    11041 tgttcttgct tccgaagttc caagctttcc ctttgtttgc ccttcgtatt gaagttgggc
    11101 agagtcaatg ttaaccgata ctattgcggt tattccttct ttgagatcgt ctcctgaaag
    11161 agcttcggcc ttatctttta ttaaattatg ttttttggcg taatcattga ttgttttagt
    11221 tagcgcgctc ctgaagccgg ttaaatgcgt tcccccctcc cctgttcgaa ggtggtttgc
    11281 aaatgttagt acgttttcgg catatgagtt gttgtattga aacgctattt caacatcgat
    11341 attctcatag tttttatgta tatggaaaat ttccgggtgc ataggttttc gtgatctgtt
    11401 tagagattta atgtaggctt ttacgccgcc ttcaaaatag aaacagtatc tctgatctgt
    11461 tctatgatct ataatgtcaa atttaatgtt tgcggttaaa taagcgtatt ctcttaactg
    11521 attaacaaaa aatttatatt taaaatccac tgtttcaaaa atggtttcgt ccggtttaaa
    11581 ttgcacaatt gttcctgact cggcatccca ctcttccagc ttcaagctcc attcagcatt
    11641 ttttactttt gaaattgggt ttttagggtc tagaggctct acttttctta atactttccc
    11701 cccgtcggca tattcctgaa taaccagctc atctcctctt tttacaatta ctcggcacca
    11761 ttctgaaagt gcgtttacta ctgaagcacc tactccgtgt aaaccgctgg atactttgta
    11821 ccctcctgca ccaaacttac ctcctgcatg aagcttagtg aaagcaagct cgagcgccga
    11881 cacaccgtag cccttttttt catcgtatgg tatacctctg ccgttatcat aaattgccac
    11941 tgatccgtct ttaaaaaact ctacttttat atagtttgca taccctgcca ttgcttcgtc
    12001 cattgaattg ttaactacct ctgtgactaa gtggtgcaag ccttgttgcc cagttgagcc
    12061 gatatacatt ccaggcctct ttcttaccgg atcaaggcct tcaagaacct gaatctgatc
    12121 agatccgtag ttgttatcca ggtcagcctt tgattttttt gttttttctt ctttcgccat
    12181 tgctttaaga ttgtatagca tgttggatta tgtttcaaat tattggagtt tgctgtagat
    12241 cgttaggttt aaatgggcaa agagaaaaac atgctatttt ataatttcgt tacttacaac
    12301 tattttcgca agctagcaga tgtagttcgc acaac
//
LOCUS       DAOSWN010000018        12020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_3375_length_12020_cov_5.137484, whole genome shotgun sequence.
ACCESSION   DAOSWN010000018 DAOSWN010000000
VERSION     DAOSWN010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12020
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3375_length_12020_cov_5.137484"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(284..1411)
                     /locus_tag="PLF29_01515"
     CDS             complement(284..1411)
                     /locus_tag="PLF29_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HXK52647.1"
                     /translation="MKIAIDAYQMATEGRVGTTTYIYNTVKNILENNQKNQTLLYAPG
                     DILDEYTLPSAKSKILPTFISWTQVSLLLELLRNNPDVILLPRQTLPVLYRPVRKLLR
                     VATLGRYRGTKLVSVIHDISGGWMRYGPTFATCVLSDQIVAVSQSTKKDMVSAFKIPE
                     DKVQVIYEGLDESIFKAGPKDVSMIKEKYSENCDYVLYVGSLIKRKNLDRLIEAFAQV
                     KESELVAEKYPKLKLVLAGGYDPKNKYAEQLLEKVSVAKDVIYVGKVPQEELGALYSG
                     ALVTALVSLKEGFGLTILESQACGTPVLTSNISSMYEISDNSCMHCNPYDTHSINNTM
                     QEIILNDPLKTELITRGLKNIKKYNWGSSASSLVRLMSKIP"
     gene            1469..2620
                     /locus_tag="PLF29_01520"
     CDS             1469..2620
                     /locus_tag="PLF29_01520"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HXK52648.1"
                     /translation="MKGKLFQSTITGAVLIGITALIYLYTSGYRISKETSRPIDFKQT
                     GMINVKSIPDGASVYVNDVLKAATNNSIPSLNPGTYKLKIVKSGFIPWEKDIEVFQEL
                     VTDITAVLVSGSPSFEPLTNTGARYPVVSPSLSKLAYFTNDLNAPGIWIIPLTQSGIN
                     LFKSTPSIAIENTARTLFSLGKSIEWSPDETELLVQDDKDIYHLIDIETRTSQTTESP
                     DTIRERWLSELLKKREDFIQKIDISDDFAQMAKSIDSLWSPDGKKFMYIQENGDQLEY
                     KTYNMEKPLPIGEKKDTLVFTIDKNSPQPIISWYADSYHLILVENYDQESKSGSIYLV
                     RIDGSNKTLLYSGKLYSSNVYSAPSGDKIIILTSFKSDEEVNLYTIGIR"
     gene            complement(2668..3390)
                     /locus_tag="PLF29_01525"
     CDS             complement(2668..3390)
                     /locus_tag="PLF29_01525"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HXK52649.1"
                     /translation="MFKLYPKVSKKTYVYKKALSGREAFVVRVASWSLLLCGLIFFTL
                     GAGPIIKDEVWYYVKERLNVKTRLANGENPGEGSVFSKYLTSQPILIEPVNRDFSIVI
                     EKIGVNAPIVADVPVSDEEAYNESLKSGIAHAAVSDYPSVEPGNVYLFAHSSLDFWKL
                     GKFATVFNLLRKLEVGDLVHVFYEDEEYIYKVVNKEILSGWNTYPITRPVIEPTLTLQ
                     TCNPPGTTINRLVVTANLIEKR"
     gene            complement(3461..3535)
                     /locus_tag="PLF29_01530"
     tRNA            complement(3461..3535)
                     /locus_tag="PLF29_01530"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3500..3502),aa:Arg,seq:cct)
     gene            3667..3754
                     /locus_tag="PLF29_01535"
     tRNA            3667..3754
                     /locus_tag="PLF29_01535"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3701..3703,aa:Ser,seq:tga)
     gene            3792..4499
                     /locus_tag="PLF29_01540"
     CDS             3792..4499
                     /locus_tag="PLF29_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52650.1"
                     /translation="MTIGFGNGVFYKINTTSLERFSEEIMSLFHKDRSLNAIEINAID
                     ESLIDYVIDTDSDFSEYQYVSLHAPVRNYKNDTDSNRILHKLEKIHHKHNLQNIVFHA
                     DIIDDWDWFNSFNKMPLSVENMDKRKNFGKSIKDMGSILDKYDFKLTLGLQHCFENDP
                     TGKLAEDFQEVFEGRVVEYHLSGYDEKEIHYPLFRTKQDEIISWIKDKGLPIIIESPF
                     DAMGEQSLELKYILERI"
     gene            4601..5017
                     /locus_tag="PLF29_01545"
     CDS             4601..5017
                     /locus_tag="PLF29_01545"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HXK52651.1"
                     /translation="MKQNLGRHDRLLRFALGAALLVIALATRSTSAALWGLFTLYEAL
                     AGWCVVYQLLGRNTCPLKNNATRLDWAKYFILGLQILTTAIILNLLASYVGLTTWYNF
                     LQNPTKVLSLDNYVFLFVAYPFILGLSTKNQLIKTG"
     gene            5152..5733
                     /locus_tag="PLF29_01550"
     CDS             5152..5733
                     /locus_tag="PLF29_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HXK52652.1"
                     /translation="MIQQSLVLIKPEAVKRGIVGEILNRFERANLKIVGAKLIVPTRE
                     LAERHYEKDDEWKIKIGKIRLEDAEKHNLDIMDLYGTTEPIEIGALVNKANHDYLVSG
                     PVLAFVLEGVNAVQKIRVLVGPTFANEAPAGTIRGDYSSESSYTSVVRKRTVFNIIHA
                     SGNEPEAKFEISLWFKPEEIISYRRVHEDLYGY"
     gene            complement(5845..6840)
                     /locus_tag="PLF29_01555"
     CDS             complement(5845..6840)
                     /locus_tag="PLF29_01555"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXK52653.1"
                     /translation="MFGRRSYSRTTKPGIVKRIIRTTVSSVVLGGFIFAISYAVKLGS
                     EINIERLASLSTPLTEKIGLSQEEVGQVAGEFVSRISDTGVHQTPDISVSAKSNKLRF
                     LVISDSHDDNINLENAINYADKEGVSFIAHLGDLTDWGDLESLNEARGVLYESSVPSY
                     VLPGDHDQGQTGDLSNFINVFESPNRVITIQGEKIVFFDNSANFTPLSQEDLAWFENN
                     IRGAKIVLLAQPLYHPIVNATFGVMGVFQGEKTPEVYDQAQLLLGYIRDSQVEVVIAG
                     DRHNFSKNSDPVRKSLTHVVSGALGDEANKLMSPRVLLVSIDTQGSVTVEDVVIE"
     gene            complement(6859..8850)
                     /gene="argS"
                     /locus_tag="PLF29_01560"
     CDS             complement(6859..8850)
                     /gene="argS"
                     /locus_tag="PLF29_01560"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HXK52654.1"
                     /translation="MQNLQHSISIKLIELVKREYSLDIDYETAVLEKPKKEEWGDLAS
                     TVALKISSKAGHSPADIAKKLCFLLNNEEFTFTFNDVVYPIFEKIDFAFPGFINFKFS
                     VPYLLNYALELSSKGLNVKDQKRPNISLKIALEHSNVNPNKAAHVGHLRNACIGQFIE
                     RVYEKMGHDVNVQYYEDDLGVQVSTSLMGTMELKDINPSDYEKFDHYAWDVYAKMESL
                     IVQDENLQKKREHLMQRLEDSKDEISVEQSKLADRILKDQLVTFGKLDFDYDVIIREK
                     DIVSLGLWNRAFEKLKTNPNVYLATSGPSKGCWMINMSGDKNEDAGSLISDSENQEEI
                     SVQEPLGGGRVIEEDKVIVRSNGVPTYTGKDIAYHMWKYGLLGLDFYYRELDIGTQKK
                     ILWTTSSEESDISKDVSFSKNDYVIDVIDVKQTYAIDVVRASLSYLGYTSEAENMTHV
                     NYGFVYLSPKTAEKMGIDTSDGKTQYAMSGRKGWGIKVDDLIEMVDSKLRAEYGDFKN
                     VNDVRNGAIKFEMLRYNTFQDIVFDLDAALSLNGFSGPYIQYTHARTFSILEKGAFQE
                     IDFYSLPTSLHEREVAVLAQLAKFSSVVLEAAYEFAPNNLCNYLFELAQKYNTFYNEL
                     PILSSPQEEKKVRMAITESVKAVITEGLFLIGITAPNRM"
     gene            complement(8941..9819)
                     /locus_tag="PLF29_01565"
     CDS             complement(8941..9819)
                     /locus_tag="PLF29_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXK52655.1"
                     /translation="MLKHLAIATLYYLILGSIELLGLYSNIQPAIKAYDVGQGDALVL
                     ITEKGEKVLVDGGPSFDLDYKLLSDFPSMDFCPIDVVVLTHPHADHLQGLYRYVLRCS
                     PNYIVFDNAIYDSKLYFDFKNEVERHVMENTSVHLTSNQFKSNLLGIEVLWPPENFDF
                     RRVDNINNVSIVLFYNSKAATPALLLGDLEKDSQSLMLREILFENEGNEIPFNYNRFS
                     GTSSKQSAVLKLAHHGAENGLNRQLFNIFSPSECLISVGEKNSYGHPSSITLKYAENF
                     CEILQTEILGDITLPL"
     gene            complement(9848..10975)
                     /locus_tag="PLF29_01570"
     CDS             complement(9848..10975)
                     /locus_tag="PLF29_01570"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HXK52656.1"
                     /translation="MLCLVRVGHYFYDVQGGLICIRERVFTSSRESSETPIAVPGSFF
                     STTAYRVRGVLFVFREKVDLFKKLTTLLVRRSLPSPHSELLLGSTLGINDLSLVPRFN
                     DVVVVTGTIHVIVVSGYNISLVSGMVTRALGSKYRRKNFIIASLGTFVYAVISGFEPP
                     VIRAWIMGTIASYGTFLGRKNDIVGVLFVSAESMLLVQPAFICSLSFQLSFLATLSLL
                     FFSFAVSRVLSFINKSRLGRLFYQDLVATVSAQILVLPLIAFSFGRISLVSFVANALL
                     LWTVPLATVYGLALLAVLYLSYFLNFWPFVFLAKIISAFVFVFLDIFIRGVYFFAAVP
                     YANIDFKMPLRVLLMYYFACFLVVLAPKFFSRLKIFSSEKA"
     gene            complement(11017..11709)
                     /locus_tag="PLF29_01575"
     CDS             complement(11017..11709)
                     /locus_tag="PLF29_01575"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HXK52657.1"
                     /translation="MSSITEYIRRNSYVVSAMVGLLIALLFTLFLLFNAAPESKNIYS
                     DDASDGTNNDNEGPKMIYVDIGGAVANPGLYGFEEGARLNDVLVQAGPFLDNVSFDWV
                     SKQLNLAEPVKDHQKIYIPFDFDIQAECSGTVVIDEIVFTPQIDNSSRNSNNERISVT
                     PVVDASDDGDPRFVNVNTASVEELDKLPGIGDVYAGRIVLNRPYANLEDLKTKADLSD
                     SVVSKISSLVVF"
BASE COUNT         4003 a         2510 c         2109 g         3398 t
ORIGIN      
        1 gttttaagtt gttacactaa acgtcagggc ttacttatga tattataagg caactgaaca
       61 tataatagta tatagtgaca ttatctaagt tattcaagag tataaaaagc attaaggaag
      121 attacttctt acatagggtg attagtacat ttttaaatgt agtactacac ttaataagac
      181 atttaaatga gttctgatac gtaaatatat ttttttgtta tttagaggtt tattttatat
      241 attaagaagg tattttattc ttaagattta gacttattgt tttttaaggt attttagaca
      301 ttaatctaac taaagaagac gctgaactgc cccagttgta tttttttatg tttttaagac
      361 ctcttgttat taattcagtt tttaaagggt cgtttaatat tatctcttgc atggtgttat
      421 ttatcgagtg tgtatcataa ggattgcagt gcatgcagga attatcgctg atttcgtaca
      481 ttgagctaat atttgaagtc aggacaggcg tgccacaagc ctgtgactct aaaattgtca
      541 acccaaaacc ctcttttaac gatacaagcg ccgtaactaa agcaccgctg tataaagctc
      601 ccagttcctc ttgaggaact tttccaacat atataacatc ttttgctact gatactttct
      661 caagcaactg ttctgcgtat ttatttttag gatcatagcc tccggccaga actagcttga
      721 gcttcggata cttctcagca actaattctg actcttttac ttgagcaaag gcttcaataa
      781 gacgatcaag attctttctc ttaatcaagc ttccaacata taaaacgtaa tcgcagtttt
      841 cgctatactt ttcctttatc atcgacacat cttttggccc ggccttaaaa atgctttcat
      901 ccagaccttc gtatataacc tgcactttat cttcaggtat tttaaaagcg ctgaccatgt
      961 ctttttttgt actttgagat acagcaacaa tttgatcaga taaaacacag gttgcaaaag
     1021 tcggaccata acgcatccaa ccacccgaaa tatcgtgtat tactgaaacg agctttgtac
     1081 ccctatacct tccaagagtt gcaacacgaa gtagcttcct cactggcctg tatagcacag
     1141 gcagggtctg ccttggaagc aaaataacat ccgggttgtt tcgtaaaagt tccaacaaca
     1201 aactaacctg agtccaagaa ataaaagtgg gtaaaatctt tgatttagca ctaggcaatg
     1261 tgtactcatc taaaatatca ccgggcgcat acaacaaggt ttgatttttt tgattgtttt
     1321 caagaatgtt tttgacagta ttgtatatgt aggtagtagt tcctactcta ccttctgtgg
     1381 ccatctgata agcatctata gctatcttca tgcagataag tatacaacaa gttgctgaca
     1441 aaatctttta gcggtagaat cactagatat gaagggtaag ctttttcaat caactataac
     1501 tggggcagtt ttaataggca tcactgcttt aatatatctc tacacatcgg gatacagaat
     1561 ttcaaaagaa acatccagac cgatagattt taaacaaacc ggcatgatta atgtgaaatc
     1621 aattccagac ggcgcaagcg tatacgtcaa tgatgtctta aaagccgcaa caaacaacag
     1681 tattcccagc ttgaatccgg gaacatataa actaaaaatc gttaaaagtg gatttatacc
     1741 gtgggaaaaa gacatagaag tttttcaaga actagttacg gatatcaccg cagtacttgt
     1801 atccggaagt ccgagttttg aacctctaac aaataccgga gccagatatc ccgttgtctc
     1861 accttcactc tcgaaacttg catacttcac aaacgatctt aacgctccgg gaatctggat
     1921 aatacctctg actcaatccg gaattaacct ttttaaatcc acgccttcaa tagccataga
     1981 aaatactgcc agaacacttt tttctctagg aaaatccatt gagtggtcgc ccgatgaaac
     2041 agaattactg gttcaagatg acaaagatat ctatcatctt atcgacatag aaactagaac
     2101 ctcgcaaaca acagaatctc cagacacaat aagagaacgc tggctttcgg aactattaaa
     2161 gaagcgcgaa gactttatac aaaaaatcga tatttcggac gactttgcac agatggcaaa
     2221 atcaatagac agcctctggt caccggatgg aaaaaaattt atgtacatac aagaaaacgg
     2281 cgatcagctt gagtataaaa catataatat ggaaaaaccc ttacctatag gcgagaaaaa
     2341 agacactctt gtatttacca tagataaaaa ttcgcctcaa ccaataatat cttggtacgc
     2401 cgacagctac catctcattc ttgtagagaa ttacgatcag gaatcaaaga gcggctcaat
     2461 ttatttggtt agaattgacg gaagcaacaa aaccctactt tatagcggaa agctatattc
     2521 aagcaacgtg tatagcgctc catcaggaga caaaataata attttaactt cttttaaatc
     2581 ggacgaggaa gttaatctct acacaatcgg tataagataa aaaaacgagt taaaacgaaa
     2641 ttataaaaca cgcaaagtct acagacccta tctcttctca ataaggtttg cggttactac
     2701 aagcctgttg atagtagttc cgggaggatt gcaggtttgc aaagttaaag tgggttcaat
     2761 aacaggacgt gtaatggggt acgtattcca tcctgataat atttctttat tcacaacctt
     2821 gtatatgtac tcttcgtctt cataaaaaac gtgcactaag tccccaacct cgagtttacg
     2881 caacaaattg aaaacagtag caaacttccc aagcttccaa aaatcaagag aggagtgggc
     2941 aaataaatat acatttccgg gttcgaccga gggatagtct gaaactgccg cgtgagcaat
     3001 tccgctcttc aaagactcgt tatacgcctc ttcatcagaa accggcacat cggccactat
     3061 aggagcgtta acacctattt tttctataac aattgaaaaa tctctattca caggctcaat
     3121 aaggatgggc tggctggtta aatattttga aaaaacgctt ccttctcctg gattttcgcc
     3181 attagccaaa cgggtcttta cgttaagtct ttcttttacg taataccata cttcatcttt
     3241 aataatcggc ccggcgccta aggtaaaaaa aataagtccg caaagcagca aagaccaaga
     3301 cgcgactctt accacaaaag cctctcgacc agataaagcc ttcttgtata cgtaagtttt
     3361 tttactgact tttgggtaaa gtttgaacat cggtaatcaa aaattagatt aacataattt
     3421 gaaacatata acacgcacgc cctattgaga aaatttagcg tggtgccccc gataggaatc
     3481 gaacctatat ttcaccctta ggagaggtgt gttctgtcca ttgaactacg agggcagatt
     3541 ttgtaaacgg cttaatctca tataccgaca atagccgcca caacttaata ccaagcaggt
     3601 atattttaac agaaaaacac taaaaatggg ggctttatga tatactcgaa actaacaaaa
     3661 acccttggag gggtgcagga gtggtttatc tggccggtct tgaaaaccgt gcgtgtgaaa
     3721 gcacacgagg gttcgaatcc ctccccctcc gccacaaaat ataactaggg ggaaaagaaa
     3781 ggttaccgaa aatgactatt ggtttcggaa acggagtttt ttataaaata aacacaacct
     3841 cccttgaaag attttcggaa gaaatcatga gtctttttca taaagacagg agcctcaatg
     3901 ctatcgaaat aaatgctatt gatgaaagct taattgatta tgtaatagac actgattcag
     3961 acttctcgga ataccaatac gtctcgcttc acgcaccggt aagaaattac aaaaacgaca
     4021 cggattcaaa cagaatttta cacaaattag aaaaaattca ccacaagcat aatcttcaaa
     4081 acattgtttt tcatgcagac ataattgatg attgggattg gtttaacagc ttcaacaaaa
     4141 tgccactatc tgttgagaat atggacaaac gaaaaaattt cggcaaatca attaaagata
     4201 tgggatccat tttagacaaa tacgacttta aactcacgct gggcctacaa cactgttttg
     4261 aaaacgaccc gaccggaaaa ctagcagaag attttcaaga agtttttgaa ggtcgggttg
     4321 ttgagtatca tttaagcggc tacgacgaga aagaaattca ctacccgctt ttcaggacaa
     4381 agcaagatga aataattagc tggattaaag ataaggggct gcctataata attgaaagtc
     4441 cctttgacgc tatgggagaa cagagcctgg agctaaaata cattcttgaa agaatttaac
     4501 ggaataccct gtgatgccca agacttcgag cgccggaaat ccacgatttt gtaaaacaca
     4561 aatataaaca aagttccctc tgaagtgata tgcttgatgc atgaaacaaa accttggacg
     4621 ccacgataga ctattaagat ttgcattagg ggcagctctg cttgtaatcg ctctcgcaac
     4681 cagaagtacc tcggcagcac tttggggcct tttcactctt tacgaagctt tagccggatg
     4741 gtgcgtggtg tatcaactgc tagggcgtaa tacctgcccg cttaaaaata acgcgaccag
     4801 gctggactgg gcaaaatatt tcatcttagg acttcaaatc ctaacaactg caataatatt
     4861 gaatttactt gcgtcttatg ttgggctaac tacttggtat aactttctac aaaatccaac
     4921 aaaagtactt tcgttagaca actatgtttt tctttttgtt gcctatcctt ttattctggg
     4981 tttatcgaca aaaaatcaac ttataaagac cggataaagc tgttcttaac caaataattt
     5041 atgctatact ccatgagaac ttgtagctga ttaagtaact tacttaaatt ttatccagct
     5101 cacacggcgt cccgcaaatt aagggaaaaa atttaaattt gggagaataa aatgatacaa
     5161 caatctttag tactaataaa gcccgaggca gtaaaaagag gaatagtcgg ggaaatatta
     5221 aacagattcg aaagagccaa tctaaagatt gtaggcgcta agctgattgt accaaccaga
     5281 gaactggccg aaagacatta cgaaaaagat gatgaatgga agattaaaat aggtaaaata
     5341 aggcttgaag atgcggaaaa acacaattta gatattatgg atttatacgg cacaactgag
     5401 ccaatagaaa taggtgcttt ggtgaataaa gctaaccacg actatttagt ttccggtccc
     5461 gtactagcct ttgtgcttga gggagttaac gcggttcaga agattagagt tttagtagga
     5521 cccacatttg caaacgaagc cccggcggga acaattagag gcgactatag ttcggaatct
     5581 tcatacacat cagtcgtcag gaaaagaaca gttttcaaca taatccacgc gtcgggaaac
     5641 gagcccgaag caaaatttga gatctctctg tggttcaagc ctgaagaaat aatctcatat
     5701 cgaagagtgc atgaagactt gtacggatat tgattaaaaa cggcgccaaa taacccaaag
     5761 ccacaataga cttaacgaac ttcaaaaatg aggaagtgat aaatagaaag taggcctatg
     5821 ggcaaaaggt tgctccttag aaaattattc tataacaaca tcttctacag taaccgagcc
     5881 ttgagtatca atgcttacga gcagtactct cggactcata agtttattcg cttcatcgcc
     5941 caaagcacca gaaactacat gagttaaaga ttttctaacc gggtcactgt ttttactaaa
     6001 attatgccta tcccccgcta taacaacctc aacctgagaa tctctaatat agccaagcag
     6061 aagctgggcc tgatcataaa cttctggagt tttttcacct tgaaaaacgc ccataacgcc
     6121 aaaagtggcg tttacaatgg ggtgatacaa aggctgagct aataaaacta ttttagcccc
     6181 acgaatgtta ttttcaaacc aagctaaatc ttcttggctt aagggagtga aattagcact
     6241 attatcaaaa aagacaatct tctcgccttg aattgtaata actctgtttg gactttcaaa
     6301 aacatttata aagttgctta aatctccggt ctgcccttga tcatggtctc cgggcaatac
     6361 atagctaggc acagaacttt cgtacaaaac acctcgggcc tcatttaaac tttccaggtc
     6421 gccccagtct gttaaatccc ctaaatgcgc tataaaagac acgccctctt tatccgcgta
     6481 atttatggca ttctctaaat ttatattgtc gtcatgagaa tcagatataa ctaaaaagcg
     6541 taacttgttg gatttcgctg acactgaaat gtcaggggtt tgatgcacgc cggtgtcgga
     6601 tattctggaa acaaattctc ctgctacctg cccaacttct tcctgagata aaccgatctt
     6661 ttctgttaat ggcgttgaga gagacgccaa acgttcaata ttaatctcgg agcccaattt
     6721 cactgcatac gatatagcaa aaatgaatcc tcctagcacc acactagaaa cagtagttct
     6781 aattatcctt tttacaattc ccggtttagt ggttcgtgag taagatcgtc gcccaaacat
     6841 tgctaatatt atacaacact acatacgatt cggggcagtt atgcctatca aaaaaaggcc
     6901 ttcggttatt actgccttta cagactcggt tattgccatt cttacttttt tttcttcctg
     6961 aggactactc agtataggga gctcgttgta aaacgtattg tatttctgtg ccaattcgaa
     7021 aagatagtta cagaggttat tgggagcaaa ttcatatgcc gcctccaaaa caaccgagga
     7081 gaatttagct aactgagcca gaacagcaac ctcgcgttca tgtaaagatg ttggtaaaga
     7141 ataaaaatca atttcttgaa aagccccttt ctccaaaatc gagaatgttc gggcatgagt
     7201 atattgaata taaggccctg agaatccgtt taagctaaga gcggcatcaa gatcgaaaac
     7261 aatatcctga aaagtgttgt atctgagcat ttcaaatttt atagccccgt tcctaacatc
     7321 attaacattc ttaaagtccc cgtattccgc ccgtagcttg ctatctacca tctctattaa
     7381 atcatctact ttaatgcccc aacctttacg gccgctcatg gcatattgtg tttttccatc
     7441 ggaagtatca attcccattt tttctgcagt tttaggagac aggtaaacga aaccgtagtt
     7501 tacatgggtc atattttcgg cctcagaagt atatcccaaa taacttaagg aggctcggac
     7561 cacatcgatg gcgtaggttt gctttacatc aataacatca ataacgtaat catttttgga
     7621 aaaagacaca tcttttgaaa tatctgattc ttcagacgaa gtggtccata aaatcttttt
     7681 ctgtgtccca atatcaagct ctctgtaata aaaatccaac cccaacaaac catatttcca
     7741 catgtggtat gcaatgtcct tgcccgtata tgtcggaact ccattagatc ttacaataac
     7801 tttatcttct tcaataacac ggcccccacc tagaggttct tggactgaga tttcttcctg
     7861 attctcgcta tcagaaatta aagatccggc atcttcgttt ttgtcaccgg acatgttaat
     7921 catccagcaa cctttagaag ggccgcttgt tgctaaatac acattagggt ttgttttaag
     7981 cttctcaaaa gcacgattcc ataacccaag agaaacaata tctttttctc taattatgac
     8041 gtcgtaatcg aagtccaatt ttccaaacgt aaccagctgg tctttgagaa ttctatcagc
     8101 aagcttggat tgctctactg atatctcgtc tttactatct tctagccttt gcattaagtg
     8161 ctcgcgtttt ttttgaagat tttcatcttg aacaatcaaa ctttccatct tcgcgtaaac
     8221 atcccacgca tagtggtcga acttctcgta gtcagaagga ttaatatctt taagctccat
     8281 tgtgcccata agagaggtgg aaacctggac acccagatca tcctcataat attggacatt
     8341 gacatcatgg cccatttttt cgtacacacg ttcaataaac tgccctatgc aagcgttccg
     8401 taaatgtcca acatgcgctg ctttatttgg attaacattt gagtgctcaa gagcaatttt
     8461 aagcgagatg tttggtcttt tttgatcttt gacatttaaa cctttagaag aaagttctag
     8521 cgcatagttt aataaataag gaacagaaaa cttaaaattt ataaatccag gaaaagcaaa
     8581 atcgatcttt tcaaaaattg gatatacaac gtcgttaaac gtaaatgtga attcttcatt
     8641 gtttaataga aaacacagtt tctttgcaat atccgccgga ctatgaccgg cctttgaaga
     8701 aattttcagt gctaccgtag aggctaaatc cccccattcc tcttttttag gtttctctaa
     8761 aacagctgtt tcataatcta tgtccaacga gtactctctt ttcacaagct caataagctt
     8821 aatcgaaatg ctgtgttgta gattttgcat ggtgtaagta tagcagaaca gaactacaag
     8881 gacagcctat ttactaacac aaagaccgcc ctaaaacgaa attttaaaat tttgctttta
     8941 tcacagaggt agagtaatat ctcctaatat ttcggtctgt agtatttcac aaaagttttc
     9001 tgcatatttc aaagtaatgc tcgagggatg tccatatgaa tttttctccc caacacttat
     9061 taaacattca gaaggagaaa aaatattaaa caactgcctg ttcagcccgt tttctgcccc
     9121 atggtgtgca agttttagta cggcactctg cttagaagac gtgccgctga atcgattata
     9181 attaaaggga atctcattcc cctcattctc aaaaagtatt tcacgtagca tcaggctttg
     9241 agagtctttt tctaagtcac ctagtagtaa agctggggtg gccgccttag aattgtaaaa
     9301 caacactatc gagacattat taatattatc aacacgtcga aaatcaaaat tttcaggcgg
     9361 ccacaacacc tcaatcccca acaaattact tttaaactga ttcgaagtta aatgaacgga
     9421 agtattttcc ataacatgcc gttcaacttc atttttaaaa tcaaaatata acttcgaatc
     9481 ataaatagcg ttatcaaaaa caatataatt aggcgagcat ctcaacacgt atctatataa
     9541 tccttgcaaa tgatctgcat gaggatgtgt aagcacaaca acatctatag gacaaaaatc
     9601 catagaagga aagtctgaaa gcaatttgta gtcaagatca aagctcggac ctccatcaac
     9661 aagaaccttt tctccttttt cagttattaa aacaagcgca tctccctgac ctacatcgta
     9721 ggcttttata gctggctgaa tattagaata caggcccaaa agctcaatag accctagaat
     9781 taaataatat aaagtagcaa tggctaaatg tttaagcatg atatcctaca taaacacaag
     9841 gttatgacta ggctttctcg gaagaaaaaa tttttaaacg agaaaaaaac tttggcgcca
     9901 gaaccactag aaaacacgca aagtaataca taagtaaaac tcttagaggc attttaaaat
     9961 ctatgttagc gtacgggacg gcggcaaaaa aatacacacc cctaataaaa atatctaaaa
    10021 aaacaaaaac aaatgctgaa attattttag ccaaaaagac aaatggccaa aaattgagga
    10081 aataacttaa ataaagcaca gcaagcaacg ccaatccgta aactgtggct aaaggcactg
    10141 tccaaagtaa taaagcattc gcaacaaaag aaacaagact aatacgtccg aaagaaaaag
    10201 ctattaacgg caagaccaaa atctgtgcag aaacagttgc caccagatcc tgataaaata
    10261 acctaccaag gcggctttta tttataaaac taagtaccct tgataccgca aaagaaaaaa
    10321 acaacaagct caaggttgca agaaaactta attgaaaact taagctacat ataaaagccg
    10381 gctgaaccaa cagcatagat tcggcagata caaataaaac ccctacgata tcattttttc
    10441 tccccaaaaa agttccgtaa gaggctatcg tccccattat ccaagcacga ataaccggag
    10501 gctcaaatcc tgatattacc gcatacacaa atgtgcctaa gctcgcaata ataaagtttt
    10561 ttcttctgta cttcgagcca agagctcggg tgaccatgcc tgaaaccaaa gatatattgt
    10621 accccgacac tacaatcaca tgtatagtgc cggttacgac cacgacgtcg ttaaacctgg
    10681 gcacgagaga taaatcattt ataccgaggg tgctgcccaa aagcagctca ctatgtggac
    10741 taggcaaaga ccttcgaact aagagcgttg ttagtttttt aaacaagtca actttttcac
    10801 gaaaaacgaa cagcactccc ctaactctat aagcagttgt gctgaaaaaa gatcccggaa
    10861 ccgctatcgg agtctctgag ctttccctcg aagatgtaaa aacacgctct cttatacata
    10921 taaggccgcc ctgaacatca taaaaataat gcccaactcg aacgagacac aacaagagaa
    10981 aagttaaatg aaaccaatgc ttctttaacc aaaacactag aaaaccacta aacttgagat
    11041 cttgcttact acgctgtcag atagatctgc tttggtcttt aagtcttcca gattagcgta
    11101 aggcctgttt aaaactattc tccctgcata tacatctcct atacccggaa gcttatccag
    11161 ctcctcgacg gaagctgtat ttacatttac aaaccgcgga tcgccgtcat cagaagcatc
    11221 cacaacagga gtaaccgaga tcctttcatt attgctgttt cgcgaagaat tatcgatctg
    11281 aggagtaaaa acaatctcat ctataaccac cgttcctgaa cattcggcct gtatatcaaa
    11341 atcgaacggt atgtaaattt tttggtgatc tttcacgggc tcggccaagt ttaattgctt
    11401 agaaacccaa tcaaaagaaa cgttatcaag aaaaggcccg gcctgaacaa gaacatcatt
    11461 caacctagcc ccctcttcaa agccgtagag gcccgggttt gcaacagctc cgcctatgtc
    11521 gacgtatatc atctttggtc cctcattgtc attattagtt ccatccgaag catcatctga
    11581 atagatattt ttgctctccg gtgccgcatt aaacaataaa aacaatgtaa ataatagcgc
    11641 tatcaaaagc ccgaccatcg cactgacaac atatgaattt cttcttatat attctgtgat
    11701 gctgctcaca aagaaaatat aacaccttaa aaaagctgtt gttcggatct gtctacacta
    11761 aatagactac tcagagcaaa gaacataatt accgcataat tattgcaaag ttatcaacta
    11821 tttagttctg acttgtggat aagctctatg cataataatt accgcagaat aaacgcaaca
    11881 acaaccagag cttttcggta aacaaaaatt aattaattag acgcgaagag attgagaaaa
    11941 tatatatctt atcaaccatc accggcttac acaggtgttt tgttgtaatt tttttgcatt
    12001 gcaaagccca acctgttggg
//
LOCUS       DAOSWN010000019        11194 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_3685_length_11194_cov_4.767573, whole genome shotgun sequence.
ACCESSION   DAOSWN010000019 DAOSWN010000000
VERSION     DAOSWN010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11194
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3685_length_11194_cov_4.767573"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="PLF29_01580"
     CDS             <1..533
                     /locus_tag="PLF29_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52658.1"
                     /translation="GMVGIALVVISILALRFGSTDIAALNYSKIALIAAGAAFGITFY
                     TWHPSKIMWGFGAMSVGSIIAALSILIGSKIAVSMLVVLLPFIDSAVTIIRRILNGQN
                     PLKSDRKHLHHLLLDRGWSIKKTALFYWATTAVFGILGLITADKNMALVIISIGGTLA
                     FIIASLNISAHRKARK"
     gene            complement(598..1077)
                     /locus_tag="PLF29_01585"
     CDS             complement(598..1077)
                     /locus_tag="PLF29_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52659.1"
                     /translation="MLLTPHTFVGVALATSLQNPFIAVPLSFAMHLLGDKVPHWDFYS
                     NSTKEERVKGWRPLAVMADLVTGVAVGLTFTYFVLWKQQNPDLALTIFLSGIASVLPD
                     ALEGPYIYMKSEPKLFSYMTKIQRRLQFQAPLPWGLLTQLLVIVFSSLIILKNLGLI"
     gene            1177..1833
                     /locus_tag="PLF29_01590"
     CDS             1177..1833
                     /locus_tag="PLF29_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXK52660.1"
                     /translation="MRGLIVEDEKQLGESIESHFKGFADCILLTSSPNNFDYIQFDFF
                     ILDIKLGNSYNEGFFLCQHIRKISPAPIIIISSRFSLKDKLKAFDLGANDYLVKPFNI
                     VELVARIKMFLAYRAQSKGTTDTIIETNKLIINKDARIAVYNNRNITLSKKEFDLLLY
                     LAENSERVLSRENITKEVWDEETDLNSNVVDVYINRLRKSTSKDLIKTVYKVGYALNA
                     "
     gene            1826..2578
                     /locus_tag="PLF29_01595"
     CDS             1826..2578
                     /locus_tag="PLF29_01595"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK52661.1"
                     /translation="MPKSRTRNTTERQDRLLRKRLVTDSEEKIEKHTDSELLKMRCVM
                     HDARNLIMLLKESLNTIKIAKKTEPILNNMHDTLKSLEYLLDADYKKNKVSTTTLQKE
                     LERSVKRLRIYFPLQTISLRIHSNKNIRINETKAALLVRCLLNLIQNSIEAEASSIII
                     VLSSKYKKVYLSVSDNGSGFSKEMLKKATSYGISTKAQTDNNEGGVGLFSVASLVLAE
                     FGGEVQIKNLNRNNDIRGAYITLVLDSKRLLS"
     gene            2685..3413
                     /locus_tag="PLF29_01600"
     CDS             2685..3413
                     /locus_tag="PLF29_01600"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003340642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXK52662.1"
                     /translation="MKKENIQTGANIQTILILLMVGLLALGISLATAKFRNNNTAQTP
                     QKPGAEKMTNLPAPKYSEKPEMQIDVNKKYKAEIETSKGTMEVILDPSFAPMTVNNFV
                     FLAREGFYDGVKFHRIIADFMIQSGDPFTKSELGVDSINWGVGGPGYAFEDENNDENY
                     LKGTIAMANSGPNTNGSQFFITHEDLTDTLPKQYTIFGRIEDEQSMKVLDEIAATKVI
                     PNAYGEVSFPAEEVTITTIRIAEL"
     gene            complement(3394..4182)
                     /gene="rsmA"
                     /locus_tag="PLF29_01605"
     CDS             complement(3394..4182)
                     /gene="rsmA"
                     /locus_tag="PLF29_01605"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HXK52663.1"
                     /translation="MPLIKAKKELGQNFLKDEFALDTMVQELYASGEIPEVTVEIGPG
                     LGALTSCLLRAGSSLLKAVEFDPRMVTYLEQKFVKEIESGKFEIIYANFLDWYPAFTL
                     SDFCPDRFSVVGSLPYYITSPIIHKLIHGKTLPEVAVLLIQKEVAEKITSAKPDSSYL
                     SVFVQTFFDVSYVITVDKKSFDPVPKVDGGVVRLSKRKNLQIEGTEIIRYEGFLHRCF
                     ASPRKMLNKVFTKTELEILNIDPTLRPQNFDAQDYIKIFKALRS"
     gene            4227..4712
                     /locus_tag="PLF29_01610"
     CDS             4227..4712
                     /locus_tag="PLF29_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HXK52664.1"
                     /translation="MTNIYIALENIRSLYNVGAIFRTASFFGIYNIFLIGYSGMDLTV
                     TGKPKLHEKLAKSALGSEKDLNTKTFESTTAFIEYCRKQKINLFSIEQCSTSEPLQSL
                     SALENDTAIVFGNEVEGVSEEMLSNSNKIFEITKFGKHNSLNVTTACGIVLYQITKIL
                     K"
     gene            complement(4763..6721)
                     /locus_tag="PLF29_01615"
     CDS             complement(4763..6721)
                     /locus_tag="PLF29_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HXK52665.1"
                     /translation="MDFLNELNEQQKEAALHVDGPALVVAGPGSGKTRVLTYRVANLI
                     FNHGISPFNILCVTFTNKASGEINERVAKLLRNLQAGSTKLAWSGTFHSICAKILRID
                     GKEINIPSTFVIYDTDDQVALIKRILKDFGLDPKQYNPNAVLATISSAKSELILSTDY
                     ANYAQGYFQRTVARIYPEYQKRLRDNNALDFDDLLVQAVLLFEKRPEVLLKYQNKFKY
                     ILVDEYQDTNKAQYVLTKNLSNGSKNIFVVGDMSQAIYSFRGANYRNILNFQTDYPDA
                     RIFNLERNYRSTQIILDAAKSIIKNNTSHIELDLWTEKVEGDLIELYTADSEIDEAGY
                     VVNKITEIVSDRTRSPLITLNDIAILYRTNAQSRNIEEQLIKNNIPYRIVGGFRFYAR
                     KEIKDITSYLKVLYNPQDTISWQRIINVPSRGIGIKGQAELEEGGWNLEEISRRTKVD
                     FVGLSLLAKDSTTLELLDVVLDKSGYIKMLNDGTEENIVRMENIQELRSVATNFPVLA
                     EFLENVALIESSDKPETYEQDRITLMTVHASKGLEFKYVFIVGMEENLFPHSRSLMEL
                     DAIEEERRLCYVAITRAGIKVFLTKAESRLFYGRRESNVSSRFLQEIPAHLLNISGKI
                     KGKSGTDERSVDDFLDELDFDRINFSWE"
     gene            6781..7305
                     /locus_tag="PLF29_01620"
     CDS             6781..7305
                     /locus_tag="PLF29_01620"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HXK52666.1"
                     /translation="MKFARIASILIVLSVVLYTLRTDQIVSIHDLSNKITKRMKRESP
                     KYDKKMVVNNIEMSVALAISKEEQTQGLMEISTLPDNAGMLFIFDQEGGWGFWMKNTL
                     IPLDMIWINKSFEIVDIQTAPPCPPEEKDCPAYRPTVPAKYVLEVNGGWAKRNNVTVG
                     NKVNIDRILSDVSL"
     gene            7429..7695
                     /gene="trxA"
                     /locus_tag="PLF29_01625"
     CDS             7429..7695
                     /gene="trxA"
                     /locus_tag="PLF29_01625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HXK52667.1"
                     /translation="MIKIVDFYADWCGPCQMMKPVFHELEKEYEGKIEFKQVDVEADN
                     ATAAKFGVMSIPTFVILKDEKEIDRRMGAMPKEMMKKWLDSHLE"
     gene            7706..8758
                     /gene="gap"
                     /locus_tag="PLF29_01630"
     CDS             7706..8758
                     /gene="gap"
                     /locus_tag="PLF29_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006198799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HXK52668.1"
                     /translation="MIKATAQPNKKTRIAINGFGRIGRAALKIALSKPEAEVVAINDL
                     SDPEALAYYLKHDSAYKNTELDISILHESDRNYMVIGSSKVAMFAEADPKNLPWKNLN
                     VDVVLECTGFFTKDGSASAHLDAGATKVVISAPAKGGNIQTFLLGVNDKQYLGQNIVS
                     NASCTTNCISPIVSIINKEMGIVKSIMTTVHAVTSTQNIVDSMPKKGSSDLRKGRAGF
                     TNMIPTTTGAAIATTEVLPELKGLFDGVSIRVPVITGSLADITMLVRKKTTVEEINSV
                     LEKAASTEGLKGVLKVTHEQLVSSDIIGDPHSAIVDASMTKVVDGDLVKVLAWYDNEW
                     GYSNRLVELALTIAQV"
     gene            8843..9208
                     /locus_tag="PLF29_01635"
     CDS             8843..9208
                     /locus_tag="PLF29_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52669.1"
                     /translation="MELNDQVIKRRLKIIDDLTTELRELKSQIDDSLENNPDLQELEE
                     QMTQVREAVKEKKQKALETESHKRLQEEIRQKRQEITENKETLSQELADYYAQSGLME
                     IEDHEGNIKKMKFSVKLVS"
     gene            complement(9252..10805)
                     /locus_tag="PLF29_01640"
     CDS             complement(9252..10805)
                     /locus_tag="PLF29_01640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXK52670.1"
                     /translation="MTKDLVTSRKKTSSYVGRMSGSLRTILNSADIWLIMVLGLFLRL
                     RQFTKSDLWFDEAYTGILMRVPKALYLSELKLDPHPPFYNIAMRYLTNVIGVSDVTLR
                     LPALVFGVLTILFIYLSIKKFIDRETALVAALLVSISPFLVEYSVEARSYSFYGFLGT
                     LALYFYTHRKYWALPVVLVPLLFTHLMAFTVVSAFFGIVFFDFVSSLLQKSSLKSQLP
                     KIFALITVFASLLYFKAYVFNYYGETTIKAQASKEWIRPVAYSNVSRSMEAYLLGVKV
                     KLPGQDTLNDVDFIFDARIWGAFLVVVYFGLVLFIVTKYIRKILSISKSDRNVLEQIL
                     AFINNEFVRLTLLFIVPQLLLIGASFYLDNNLYVERYLFPASIFFLASFAYLIVKSLS
                     FEIRAFVVLGYFLLVQAIIPHPYLFGMKRVATALQDFDKEIVFLAPVEYTVGRYYLGE
                     NRTLRLYSPEDPLNTYNHWPFIEGDLRPKDPSSAIFVATKYQSVPPTFMKLEREDLNT
                     GDYILYIQK"
BASE COUNT         3835 a         2226 c         2097 g         3036 t
ORIGIN      
        1 gcgggatggt aggcattgct cttgttgtca tttcaatctt agctcttaga ttcggttcaa
       61 cagatatcgc ggcgttgaat tattctaaaa tagcacttat tgccgcggga gcagcttttg
      121 gaattacttt ttacacctgg cacccatcta aaattatgtg gggttttggg gcaatgtctg
      181 tagggtcaat aattgccgcg ctgtccatac ttataggaag caaaatagca gtttcaatgc
      241 ttgtggtatt actacccttt attgattcgg ccgtaacaat catacgaaga atattaaacg
      301 ggcagaatcc tcttaagtca gatcgaaaac acttacacca cttgctactg gatagaggct
      361 ggagcataaa gaaaaccgca cttttttatt gggctacaac ggcagtattt ggtattttgg
      421 gcctgattac tgccgataaa aacatggctc ttgtaattat tagcattggc ggaacattgg
      481 cttttattat tgcttctttg aatatttcgg cacaccgaaa agcccgtaaa taaaagcgac
      541 aatcaaggca cacttcataa atttatggga aatcgcagaa agtcagttta acctagttta
      601 aataagtccc agatttttta atattattag ggagctaaat acaataacaa gcaactgagt
      661 taacaacccc cacggcaaag gggcttgaaa ttgaagcctt cgttgaattt ttgtcatata
      721 agagaaaagc tttggctcac ttttcatgta tatgtaaggg ccttcaagtg cgtcaggaag
      781 aaccgaagcg atgccagata aaaatatggt aagcgcaaga tcggggttct gctgtttcca
      841 taaaacaaaa taggtaaatg ttaaacccac agctacgcct gtaaccaagt cggccattac
      901 tgccaagggt cgccagcctt taactctttc ttcttttgtt gaatttgaat aaaagtccca
      961 atgaggcacc ttatcgccca acaaatgcat ggcaaaagat agtggtactg ctatgaaagg
     1021 gttttggagt gatgttgcta aagctacccc tacaaaagta tgcggtgtta ataacataat
     1081 acaatgaaac tatataactt ggggggtatt tttgcaagac cggccttgca cagttaaatc
     1141 taataaacta gaattggtat aaatattcta atatacatga gaggattaat agttgaggac
     1201 gaaaaacaac ttggcgagag catagaaagc cattttaagg gttttgccga ttgtatatta
     1261 ctgacttctt ccccaaataa ctttgattac atacaatttg atttttttat tttggatatt
     1321 aaactgggga atagttataa cgaagggttc ttcctgtgcc aacatattag aaaaatctct
     1381 ccggctccga taataataat atcgagcaga tttagcctta aagataaact gaaagcattt
     1441 gacttaggtg ccaacgatta tttagtaaaa cctttcaata ttgtggagct tgtggctaga
     1501 ataaaaatgt ttttagctta tcgggcacaa agcaaaggaa cgaccgatac tattatcgaa
     1561 acaaataaac taataataaa taaggacgcg aggattgcag tctacaacaa cagaaatatt
     1621 actttatcaa aaaaagagtt cgatctgctt ctctaccttg cagaaaattc tgaaagagtc
     1681 ctatcaagag aaaacataac aaaagaagtg tgggacgaag aaacggacct caacagtaac
     1741 gttgttgacg tgtatataaa tcgactgcgc aaatcaacaa gcaaggactt gattaaaaca
     1801 gtttacaaag taggctatgc attaaatgcc taagagcaga actagaaata caacagaaag
     1861 gcaggatcgc cttctgagaa aaaggctggt aaccgattct gaagaaaaaa ttgaaaaaca
     1921 cactgactca gaattactaa agatgagatg cgtaatgcat gatgcaagaa atttaattat
     1981 gttgttaaag gaaagtttaa acacaataaa aatcgctaaa aaaacagagc ctatcttaaa
     2041 taacatgcac gataccttaa agagtctgga atacttattg gacgcagatt ataaaaaaaa
     2101 caaggtttcc acaacaaccc tacaaaaaga actggaaaga tctgtaaaaa gattaagaat
     2161 atattttcct ctacaaacta tctcgctgcg catacatagt aacaaaaaca ttcggataaa
     2221 cgaaaccaaa gcggccttgc ttgttaggtg cttgcttaat ttaatacaaa acagcataga
     2281 agcagaggca tcctcaataa ttatagtttt atcctccaaa tataaaaaag tgtatctatc
     2341 ggtgtcagat aacggaagtg gtttttcaaa agaaatgctg aagaaggcaa cgtcgtacgg
     2401 gatttcaaca aaagcacaaa cagataacaa cgagggaggc gtgggtttat tttcagtcgc
     2461 gtctttagta ttagcagaat tcgggggaga agtacaaatt aagaatctga acaggaataa
     2521 cgatatcaga ggggcctaca taacgctggt gcttgattct aagaggttat taagctaagc
     2581 tagttacatg atctgtggat cggatcatat cgatatccgg acataaattc ctgcccggag
     2641 aaggggtccg gaagaccaag ggtctccgga cccccaaaac aaaaatgaaa aaagaaaaca
     2701 tacaaacagg agcaaatata caaacaatat taattttact tatggtgggg ttactagctc
     2761 taggaatcag tcttgcgact gccaaattcc gaaacaataa cacagcacaa actccccaaa
     2821 agccaggagc tgaaaaaatg actaacctac ccgcacctaa atactcagag aagccggaaa
     2881 tgcaaataga cgtaaacaaa aaatataaag ctgagattga aacttcaaaa ggaacaatgg
     2941 aggttatact cgacccaagt tttgctccca tgactgtcaa caactttgta tttcttgcaa
     3001 gagagggctt ttatgacggc gtaaaatttc atagaatcat tgctgacttt atgattcaaa
     3061 gtggggatcc attcacaaaa tccgaactag gggtagacag catcaattgg ggagtcggtg
     3121 gaccaggata cgcttttgag gacgaaaata atgatgaaaa ctacttaaaa ggaacaatag
     3181 ctatggcaaa cagcggacca aacacaaacg gaagtcaatt ttttataact cacgaagacc
     3241 ttaccgatac ccttcctaag caatatacga ttttcgggcg tattgaagac gaacagagta
     3301 tgaaggtttt agatgagatt gccgcgacaa aagttattcc aaacgcctat ggagaagtct
     3361 ctttcccagc tgaagaagta acaattacaa ctatcaggat cgcagagctt taaatatttt
     3421 tatataatct tgagcatcaa agttttgagg ccttaaagtt ggatctatat ttagaatttc
     3481 gagttcagtc ttagtaaaaa ccttatttaa catttttctt ggagaagcaa agcaacggtg
     3541 taaaaaaccc tcatacctta ttatttccgt gccctcaatt tgcagatttt ttctctttga
     3601 tagccttaca actcccccat caaccttggg aacaggatca aagctctttt tatccacggt
     3661 tattacatat gaaacatcaa aaaaagtttg cacaaaaaca gataaatagg aactatccgg
     3721 tttagcggag gtgatttttt cagcaacctc tttttgtatc agaagaacag ccacctcagg
     3781 caatgtttta ccgtgtatta atttatgaat aatgggcgac gttatgtagt aaggcagaga
     3841 accaacaaca ctaaacctat ccgggcaaaa atcacttaaa gtaaaggccg ggtaccagtc
     3901 taaaaaattt gcgtagataa tctcaaactt tccgctctca atctctttaa caaatttctg
     3961 ctctaaatac gtcaccattc ttggatcaaa ctcaacagct ttaagcaagg aagacccagc
     4021 acgcaagagg cacgatgtta gagcccctaa acccggtcca atctctactg tcacttctgg
     4081 aatttcgccc gacgcataaa gctcttgaac cattgtatct agagcaaact catcttttaa
     4141 aaagttctgc ccgagttctt tttttgcttt tatcaaaggc atattatgac ttaattttat
     4201 catgaatttt gtagtatact tcatgcgtga caaacattta catcgcccta gaaaacataa
     4261 gaagcctcta caacgtaggt gcaattttta gaaccgcatc tttttttgga atttacaaca
     4321 tatttctaat tggatactcc ggcatggatc ttaccgtaac gggaaaaccc aaattgcatg
     4381 agaaactggc gaaaagcgct ctcgggtccg agaaagattt aaacactaag acttttgaat
     4441 caactacagc ttttatcgaa tactgtcgca aacaaaaaat aaacttgttc tccatagaac
     4501 aatgctccac cagtgagcct ttacaatctc tgtctgcact agaaaatgat acggcgatag
     4561 tcttcggaaa tgaagtagag ggcgtgtctg aagaaatgct ctcaaattct aataaaatat
     4621 ttgaaattac taagttcggc aaacataact cactgaatgt gactacagca tgtgggattg
     4681 ttctttatca aataacaaaa atccttaaat aaagagatta gactttctcg caacaagact
     4741 aactgtatct ttcatcctgc agctattccc agctaaaatt aattcgatcg aaatcgagct
     4801 cgtctaaaaa gtcgtctaca gaccgctcgt ccgttccgct cttgccctta atttttcccg
     4861 aaatgttaag aaggtgcgca ggtatttcct gcaggaatct gctggaaaca ttactttctc
     4921 tacgcccata aaacaacctc gactctgctt tggttaaaaa aacctttata cctgctctgg
     4981 ttattgccac atagcacagc ctgcgctctt cctctatagc atcaagctcc attagcgagc
     5041 gcgagtgagg gaagagattt tcctccatac ccacaataaa cacatactta aactccaagc
     5101 cctttgaagc atgaacagtc ataagggtta ttctatcttg ctcatacgtt tcaggcttgt
     5161 cgctactttc aatcaacgcc acattttcta aaaactcagc gagaacagga aaattagtgg
     5221 ccactgaacg tagttcttgt atattttcca tacgaactat attctcctca gtgccgtcgt
     5281 ttaacatttt tatatagcca cttttatcca gcaccacatc taagagctca agggtagttg
     5341 agtccttggc caacaagctc aaaccaacaa aatctacctt cgttcttcta cttatttcct
     5401 caagattcca tcccccttct tcaagctcgg cctgaccttt tatacctatt ccccgagagg
     5461 gtacgttaat aattctttgc catgaaattg tatcttgtgg attatacaga acctttaaat
     5521 acgaggttat atctttgatt tcttttcttg cataaaacct gaaaccccct acgattctat
     5581 acggaatgtt attttttata agctgctctt caatgtttcg agactgagca tttgttctat
     5641 ataaaatagc aatatcattt aaggttatga gaggagacct agttctatca gaaactatct
     5701 cggttatctt attaactaca tagccggcct cgtcgatctc agagtctgcg gtataaagtt
     5761 ctatcaaatc gccctcaacc ttctcggtcc aaagatcaag ctcaatgtgg ctagtattat
     5821 ttttaataat gctttttgcc gcatctaaaa ttatttgagt cgatctatag ttacgctcaa
     5881 gattgaaaat acgagcatca gggtagtctg tttgaaaatt taaaatattc ctgtaatttg
     5941 cgcctcgaaa actataaatg gcctgcgaca tgtcacctac aacaaaaatg tttttggatc
     6001 cgtttgacaa atttttggtt aacacgtact gtgctttatt tgtatcttgg tactcgtcta
     6061 ctaaaatgta tttgaattta ttttgatact ttaacaacac ttccggtctt ttctcaaaca
     6121 ataaaacggc ctgcaccaac aaatcatcaa aatctagagc gttgttatct ctaagccttt
     6181 tttgatactc agggtatatt cttgcgacag ttctttgaaa atatccctga gcgtaatttg
     6241 cataatcggt cgacaaaata agctcagact ttgcgctgga aatagtagca agcactgcgt
     6301 tcggattata ctgcttaggg tcaaggccaa aatctttaag aatacgtttt attaatgcga
     6361 cctgatcgtc ggtatcgtaa ataacgaaag tgctgggtat atttatttcc ttgccgtcta
     6421 ttcttaatat tttggcacaa atagaatgaa aggttcccga ccaagcaagc tttgtacttc
     6481 ctgcttgaag atttcttagc aactttgcca cacgctcatt tatctctccc gaagctttat
     6541 tagtaaaagt tacgcacaaa atattgaagg ggctaatgcc gtgattaaaa attaaatttg
     6601 caactctgta ggttaaaact cgtgtttttc cgcttccagg gccagcaaca accaacgccg
     6661 gcccatcaac atgaagcgct gcctcttttt gctgttcatt taattcgttt agaaaatcca
     6721 ttattttttt attaactatt gaaacttcac aacacgaacc aaaagctata atattgaata
     6781 gtgaagtttg ctagaatagc ttcgatttta attgttttgt ctgttgtatt atacacgcta
     6841 agaacagacc aaatagtatc aatacatgat ttatcaaata aaattacaaa acgcatgaaa
     6901 cgagagagcc caaaatatga caaaaaaatg gtcgttaaca atatagaaat gagtgtggct
     6961 ttagcaatat caaaagagga gcaaacacaa ggcctgatgg aaatttcaac actgcccgat
     7021 aacgccggaa tgctgtttat atttgatcaa gaaggtgggt ggggattttg gatgaagaac
     7081 acgcttatac ctctggacat gatctggata aataaaagtt ttgaaatagt ggatattcaa
     7141 actgcacccc cctgcccccc agaagaaaaa gactgccccg cctacagacc cacagttccg
     7201 gcaaaatatg ttctagaagt aaacggggga tgggccaaaa gaaacaatgt cacagtggga
     7261 aataaagtta acattgatag aattttatcg gacgttagcc tttaatacag gcctcttggc
     7321 aaagctccag aatcaaattc ttaggttcga attataaaaa aacaaaactt atgatatgat
     7381 ttgtatgttt caacctaaaa aagtaattaa atttcggaga atataacaat gattaaaata
     7441 gtagattttt atgcggactg gtgcggcccc tgccaaatga tgaagcctgt atttcacgaa
     7501 ctggaaaagg aatacgaagg caaaatagaa tttaaacagg tagatgtaga ggccgacaac
     7561 gccactgctg caaaatttgg tgttatgagc ataccaacct tcgtcatact caaagacgaa
     7621 aaagagattg atagaagaat gggcgccatg ccaaaagaaa tgatgaaaaa atggctcgac
     7681 agccatctgg aataaacttg caaatatgat aaaagccact gcccagccaa acaaaaaaac
     7741 cagaattgca attaacggct tcggtcgtat aggacgcgcc gcattaaaaa tagccctctc
     7801 aaagcccgaa gccgaagttg tagccataaa cgatctttcc gaccccgaag ctttagcata
     7861 ctacctcaag catgactctg cgtataaaaa caccgagctt gatatatcaa tacttcatga
     7921 aagtgaccga aactatatgg ttataggttc gagcaaagta gcaatgtttg cagaagccga
     7981 tcctaaaaat ttgccatgga aaaaccttaa tgtagatgtg gttttggaat gcacaggatt
     8041 ttttacaaaa gacggctctg cttctgccca ccttgatgcc ggagcaacaa aagtcgtgat
     8101 ctcagcacct gcaaaaggag gaaatattca aacctttctt cttggagtaa acgacaagca
     8161 gtatttgggc caaaacatag tgtctaacgc ttcctgcact acaaattgta tttctcccat
     8221 agtttcaata ataaataaag agatgggcat tgtaaaatca attatgacaa cggtacacgc
     8281 cgttacttcg acacaaaata tcgtagacag tatgcctaag aaaggctctt cagatctgag
     8341 aaaaggccgt gcgggcttta ccaacatgat tcctacaaca accggggctg cgatagcaac
     8401 caccgaagtc ctgcctgaac ttaaaggtct ttttgacggc gtatccataa gagttcctgt
     8461 aattaccggc tcactcgccg acataactat gcttgtaaga aaaaagacta cagtcgaaga
     8521 aattaacagc gtcttagaga aagcggcctc tacagaaggc ttaaaagggg tgctgaaggt
     8581 tacgcacgaa cagcttgtct catcagacat aataggtgat ccgcactcag ctattgttga
     8641 tgcttcaatg acaaaagtcg tagacggcga ccttgttaaa gttttggcct ggtacgacaa
     8701 cgaatggggc tatagcaaca gattggttga gctcgcgttg actatagcac aagtataatc
     8761 tagaatttta caacaaagtt tataatttaa agaaagagtt gggccctgta aactgctcac
     8821 agagcaatca acgcaaatta aaatggaact gaacgaccaa gtaataaaac gcagattaaa
     8881 aataattgac gacctcacaa cagaacttag agagctaaaa tcccaaatag atgactctct
     8941 tgaaaacaat ccggaccttc aggaattaga agagcagatg acgcaggtac gcgaggcagt
     9001 taaagaaaaa aaacaaaaag cattagaaac cgaatctcat aagagattgc aagaagagat
     9061 aaggcaaaaa aggcaggaaa tcactgaaaa caaagagacc ctttcgcaag aacttgcaga
     9121 ttactacgca caaagcggcc taatggaaat tgaagatcac gaaggaaata ttaaaaaaat
     9181 gaaattttca gttaagctgg ttagctaaac cttatcttat tgacgacaca agtactggaa
     9241 aggtgtaaag gctatttttg tatataaagt atataatccc ctgtattcag gtcctcacgc
     9301 tcaagtttca tgaatgtggg gggcacgctt tggtacttag tagcaacaaa aattgccgaa
     9361 gacggatcct tgggccttaa atctccttcg ataaacggcc aatgattgta agtattaagt
     9421 gggtcttcag gactgtaaag cctcaaagtc ctattctctc ccaagtagta tcgtccaacg
     9481 gtatattcaa ccggagctaa aaaaacaatt tctttatcaa aatcttgcaa ggccgtagct
     9541 actcgcttca ttccaaaaag atacggatgc ggaattatag cctgtactaa taaaaaatac
     9601 ccaagaacaa caaaagccct gatttcgaac gataacgatt ttacaattaa ataagcgaac
     9661 gaggccaaaa agaatattga agcaggaaat agataccgct caacatacaa gttgttatcg
     9721 agataaaagc tcgcgcctat tagaagtagc tgtggcacaa taaataacag agtcaaacga
     9781 acaaactcgt tatttataaa agccagaatc tgctcaagca catttctatc gctcttggaa
     9841 atactaagaa tttttcgaat gtatttcgta actataaaaa ggactaagcc aaagtaaaca
     9901 accaccaaaa aagctcccca tatgcgggcg tcaaatataa aatctacgtc atttagcgta
     9961 tcttgcccgg gcaacttaac cttcacgcca agaagataag cttccatgct gcgcgaaacg
    10021 tttgagtaag ctaccggcct gatccattct ttgcttgctt gggcctttat tgtagtttca
    10081 ccgtaataat tgaaaacata tgccttaaaa tacaacaggg aagcaaaaac tgtaatcaaa
    10141 gcgaaaatct ttggcaactg agattttagc gatgatttct gtagtaatga agaaacgaaa
    10201 tcgaaaaaaa caataccaaa aaaggctgag acaacggtaa aagccatgag atgagtaaaa
    10261 agaagcggca ctaaaacaac cggcaatgcc caatacttac ggtgcgtgta aaaatataaa
    10321 gccaaagttc ctaaaaatcc gtaaaaacta tacgaccttg cctccaccga atattcaact
    10381 aaaaatgggc tgatgctaac cagcaaagcc gcaaccaaag ccgtttctcg atctatgaat
    10441 ttttttatac ttaaatagat aaaaagaatc gtaagtaccc caaaaaccag agccggcaaa
    10501 cgcaaagtta cgtcagagac accaataaca tttgtcaggt accgcattgc aatattataa
    10561 aaaggagggt gcggatcgag cttcagctcc gacaggtaaa gtgctttagg aacgcgcatc
    10621 aaaatccctg tgtatgcttc atcgaaccat aaatcagatt tagtgaattg tcttaatctt
    10681 aaaaagaggc ctagcaccat aataagccaa atatcggcgc tgtttagtat cgttcttaga
    10741 gagccgctca ttcttccaac atacgaggaa gtcttctttc tgcttgtcac aaggtctttg
    10801 gtcacagtat tattttatca cagcaaagga agcaaaagcg catgtagctc ccgttctata
    10861 acctacggcg tcaaacagaa aagtatttgg ttatattgct gccctctgct ataatgccta
    10921 ttgctatgtc aataaagaaa aagaaaaacg acgcacaaaa taataagatg gctcgccgtt
    10981 caagaaaaaa atcaaaacga ataactacta tgtagtctct aacttgtagc gcccgggccg
    11041 gcttttaaat attccggcct tatccagcca aaatgataaa gcaataaaaa gagtaaatgc
    11101 ttatataaat ctctgtggtc gaggtctgtt ttagttaaat ttataatttc gttacttaat
    11161 actatttacg caagatagta gatgtagttt gccc
//
LOCUS       DAOSWN010000020        10402 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_4054_length_10402_cov_5.006379, whole genome shotgun sequence.
ACCESSION   DAOSWN010000020 DAOSWN010000000
VERSION     DAOSWN010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10402
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4054_length_10402_cov_5.006379"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..493)
                     /locus_tag="PLF29_01645"
     CDS             complement(14..493)
                     /locus_tag="PLF29_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52671.1"
                     /translation="MDLVNRKSALWQYLPSEIKGLIEDGEKILAHAQEHQENLSDFSY
                     LVFPFSKAYEGFLKRLFLDLDLIREDEYYGDEIRIGRILNPHFMKEKGNVFNKLCSKT
                     GNGNDIVQQLWETWREGRNEVFHYFPHNFRRLSYKEASKIIQDILRAMESAVGSCYL"
     gene            550..3216
                     /locus_tag="PLF29_01650"
     CDS             550..3216
                     /locus_tag="PLF29_01650"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HXK52672.1"
                     /translation="MFHTLTLQELLQKLNTSIESGLRPEEIEKRLAEFGPNSLPEPKK
                     ISTFQKILSQFSDVLVLVLIAAAIVSFAIGEHIDAVVITTIVLINAFMGYIQEARAEK
                     AIEALKKMTTDYAKVIINGELTSVLVERLVPGDIIILESGDRVPADARLIEVANLQIS
                     EAVLTGESNPVNKHINPILQEDLALGDRKNMVYKNTTVVFGRGKAVVTGTGKDTEIGK
                     ISMLLSESDKEDTPLSKELAVVGKRLSSLAGLIILVIFTFAFLSHELDIKDSFLTAIS
                     LAVAAIPEGLPAIVTVILATGVSQLAKSKAIVRRLQAVETLGSTSYILTDKTGTLTQN
                     KMSATNVATLNENYTITQIRNDGFTFLDSKKDPVSPNQQEDLKLILLNSILCNDAEAS
                     SGTSNDSNSYIGDPTETALIELGIRAGLNIKETRKSYNRLYEIPFSSETKRMVVVTSD
                     PENSDEAYVFAKGAPEIIQEMVPTENEIIENINNNYAAKGLRSLGLAYKKISRHDLAQ
                     VLEGNKDDLSLIDDLEFIGIVAQKDPLRPEVKDALNMALAAGIKTIVLTGDHKLTATT
                     IAKELGLIRNDDEVMDGPDLAKLDDQEIINALKTVKVFSRVSPEQKLRIVQVIKGMGN
                     ITAVTGDGVNDAPAIKAADIGISMGISGTDVSKEVSDMVLQDDNYATIVKAIKEGRVI
                     YDNLIKFISYLISCNMSEIFVVGLAMVAKLPLPMLPIQILWINLITDGLPALALGMEP
                     GEKDIMLRQPRKQGNLLTKKRWVRMTIQGASMTVGVFGVFLFALRISPEAAQSAAFTA
                     LAMSQLFHAFNSRSEHHSIFSRTVPRNNFLIATFVLSVFLQLMTLYSWLGNTYLKTTP
                     IPISLFILALLSATIPVVVTEIYKLLQNRAKA"
     gene            3299..4243
                     /locus_tag="PLF29_01655"
     CDS             3299..4243
                     /locus_tag="PLF29_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HXK52673.1"
                     /translation="MDLFFFLSLVLGASVMLIKSVSWFTESSAKIAHHLKISEYTISF
                     FLIAIGTSLPEAFVAIRSGLAQDSILSFGTSMGSNIALVTLVIALPVLLTGSISTRYI
                     IASRDMYIASFLALLPFILSLDGVIGRYDAITLFAGYLLYSIAILKRARGVESLIDKI
                     EEINLLKEAFLFIFSLALLLAASNIIVISAINISETLNISLGFVGLTITALGTSLPEI
                     SFTIAAVKKGQTEEIMGDIIGSVVANSTLVLGLAAIFYPIKIDGINMGAITTFFVVLS
                     FLMFMRLARTQQKVTKKEAIVLLGIYTTFIFSELIIRL"
     gene            complement(4303..4824)
                     /locus_tag="PLF29_01660"
     CDS             complement(4303..4824)
                     /locus_tag="PLF29_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52674.1"
                     /translation="MNSQKGYSVPLILIFVFSLVSIPVYYFFINQQAAETVKGANTKA
                     TTEPGFGVQISSKSGTWDLVEYLCKDYDSCVSNLTAGTRMGLVSGGVSDLHEVVVSAQ
                     DDWSNYSYIKFYVRPSWHSDPINYRVVSLGSIPFSKAVEISDGTRNVEVVVAPLENIS
                     STYIQSAHFSDSN"
     gene            5090..6349
                     /locus_tag="PLF29_01665"
     CDS             5090..6349
                     /locus_tag="PLF29_01665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXK52675.1"
                     /translation="MQIGQKDEKFSNVEGTPSQKTQAAKAPKIAFVHEYLVQYGGAQK
                     TLEAMLEVFPNSPIYTGLYDKKSMSSFLNNQTIHSTQNKLLSKFQKYLTFLMPLVFEG
                     MDLQDFDIIISDGTAWPKGVLTNTHQLHISYIHTPPRFLYGYSVESQKRDKWYFKPIL
                     KVVDNILRVWDYNAAQRPDFLLTNSFETLARIKKFYGREAKVIYPPVELSYNNPETSS
                     EEKIIAEEQEQNTSKNYYIMLGRLSAYKNFDVVIKAFNKNGKNLVILGTGYEENYLRS
                     IAKTNITFIGRATDKQKSYYLENCKGLINAVQDEDFGIVPIEAMAYGKPVLAHRSGGH
                     LETIVEGLSGMFFESLEVGDLIKSIESFDKSIEEGLFDAGSIKKSVQKFDKEIFKKDL
                     KDFVLSKWEEKLNAGATRSSHHSSRNQ"
     gene            6303..7160
                     /gene="mutM"
                     /locus_tag="PLF29_01670"
     CDS             6303..7160
                     /gene="mutM"
                     /locus_tag="PLF29_01670"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HXK52676.1"
                     /translation="MPELPEVHTIVQGINKSLLGYKILEVRDIKKKYNLLSQEDIDSK
                     VINATIVSAERLAKQIVLNLDNQHSLSFHLAMTGKLLLREPGHPQDKAERLVLNLIYQ
                     NGARELRFCDTRLFGKVKILHSQEIEKSKNNFGLDPTTKEFNSKNFYNVIKSKNTSIK
                     NLLMDQRKIAGLGNIYATDALWLANINPTTHTSQITKEQASKLLDSVISVLNEGIAHR
                     GISMRDYVDLYGEKGSHQNHFKVYQQSHCSRCQCEIQTVRINGRATYFCPLCQPHKDK
                     TLNKKISVN"
     gene            7176..8240
                     /gene="recF"
                     /locus_tag="PLF29_01675"
     CDS             7176..8240
                     /gene="recF"
                     /locus_tag="PLF29_01675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HXK52677.1"
                     /translation="MLITKIKLTNYRNHELLELKFNGQSFIIKGPNGIGKSNILEAIH
                     LVSTTKSLRTKYDRDLINHNADVAHIEIVVKDQGSKPQLAFEEELMLSIQKSQTYPNA
                     SKKVVKINKVPKALRDFSGKIKSVLFTPDDLDILSGPPSDRRKYIDAIMFQSNESYKS
                     AHLKYTKAIRHRNKILEKIAKTGHGHNLLPVWNEIILLNGEIIQKNRRELFEILGKKA
                     QVYLEELDPQNSRLKINYLENHISEKRLEEYSQKEIYTRSTLVGPHRDDFEVDYNNYN
                     SAFFSSRGQQRTIMLAIKMAEMDHIAQATGIRPILLLDDIFSELDPKHKEAVMNITNL
                     QQTIITTAIDSKNGLEIGAL"
     gene            8444..9415
                     /locus_tag="PLF29_01680"
     CDS             8444..9415
                     /locus_tag="PLF29_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52678.1"
                     /translation="MFSPEYTITSDILKNVSGIEYAKAIIENTPLLSNWENQLIVDAK
                     TRTISYAIQKDGTNINPEDIKKLITNLPTKVPHIAERYLSTLELSHDYASTKELTEED
                     IADLYKALTGTSKYRNTPSQKGVPADEILAQMVELVDWLQSVEALQTHPMLLAYIVKL
                     NIEIIQPFEKHNSLLADLISDVLLESHGYNIKGYFCPQEYEYKTYLDYQELIDDARKS
                     YDLTQWLEYTTDIAHRELSTVADKIKMLAKDAKIAKAAGRAKLSQRHERIISYLQDYV
                     LIQNKDFPALFPNLSEDTILRDLKKLIDMGIVVKLGSTKSSRYELSN"
     gene            9454..>10402
                     /locus_tag="PLF29_01685"
     CDS             9454..>10402
                     /locus_tag="PLF29_01685"
                     /inference="COORDINATES: protein motif:HMM:NF016553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52679.1"
                     /translation="MQFGDYSKYLQRLENTSARLEITSILAEMIEILEPEEIKQAVYL
                     SLGYLDAPFKSIKFNMAEKLALRAISIAYNIAETDVENLYNDLGDLGLAAEKLSLKHQ
                     GQEPIKKQTVVEVYNALLKIANASGTGSQDKKIITTAELLKSTDSVSAKFIIRIILGT
                     NRLGFTELTILDALAKVLGKNKKFGKSIEEKYNTHPDIGYVTYLIKTKGEKGLRELDI
                     EIGIPILAQKCQRLSGVEEILEKMGEVYAEYKFDGTRVQLHMDRSVLNTARKSLFEEL
                     KEDKGFLIKTYTRNLEETTHQYPEIVTAANKQIKAESAIL"
BASE COUNT         3708 a         2102 c         1925 g         2667 t
ORIGIN      
        1 aatctcttat attctacaag tagcagcttc caactgccga ctccatggca cgtaaaatgt
       61 cttgaataat tttagacgct tctttgtaag acaggcgcct gaagttatgg ggaaaatagt
      121 gaaaaacctc attacgtcct tcacgccatg tttcccaaag ttgctgcact atatcgtttc
      181 cattacctgt tttagaacac agcttattaa aaacatttcc tttttctttc ataaaatggg
      241 ggtttaaaat acgcccaatt cgtatctcat ccccgtaata ctcatcttca cgaattagat
      301 ctaaatcaag aaataacctc tttaaaaatc cttcgtatgc ttttgagaac ggaaacacta
      361 agtaggaaaa gtccgaaagg ttctcttgat gctcttgagc atgagccaag attttttccc
      421 cgtcttcaat gagacctttt atttcgcttg gcagatattg ccaaagggca gatttgcgat
      481 tcactagatc catacattaa tagtgtatca gaaatttttt tgaatatcat gatgtataat
      541 tattaggcca tgtttcacac actcacgctt caagaattac tacaaaaatt aaacacttct
      601 atagaaagcg gcttaaggcc cgaagagata gaaaagaggc ttgctgaatt tggtcccaac
      661 tcattaccgg aacctaaaaa aatctcgact ttccaaaaaa ttctatctca attttctgac
      721 gtactagtgt tggttttaat agccgccgcc atagtctcat ttgcaatcgg cgaacatatt
      781 gatgccgtag ttatcacaac tatcgtactc attaatgctt ttatgggata cattcaagaa
      841 gcgcgggctg aaaaagctat agaggccttg aaaaaaatga ctacggacta cgctaaagtc
      901 ataatcaacg gtgaacttac aagcgtattg gtggaaaggc ttgttcccgg agacataata
      961 atcctggaaa gcggcgacag ggttcctgca gacgcaagac taatagaagt cgcaaactta
     1021 caaatttccg aggcggttct aacaggagaa tctaacccag taaacaaaca tataaaccca
     1081 attctccagg aagacctcgc ccttggtgac agaaaaaaca tggtatataa aaacacaact
     1141 gtcgtttttg gccgtggaaa ggccgtggtt acaggaacgg gcaaagatac tgagatagga
     1201 aaaatatcaa tgctgttgag tgaaagcgac aaagaagata cccctcttag caaagagctt
     1261 gccgtggtag gaaaaaggtt aagctctctt gcaggcctaa tcatattagt tatttttact
     1321 tttgcttttt tatcacacga actggacatt aaagattctt ttttaactgc aataagcttg
     1381 gctgttgcgg caatcccgga gggattgccg gcaattgtaa ccgtaatctt ggctacagga
     1441 gtttcacaat tagcaaaaag caaagcaata gttagaaggc ttcaagctgt tgaaactcta
     1501 ggatccacaa gctacattct tactgacaaa acaggaactc taacccaaaa caaaatgtca
     1561 gcaacaaatg ttgcaacact taacgaaaac tatacaataa cgcaaataag aaacgacggt
     1621 ttcacctttt tagacagcaa aaaagatccg gtatccccta accagcaaga agatcttaag
     1681 ctaattctat taaattcaat tctgtgtaat gacgctgaag cttcttcggg cactagcaac
     1741 gacagcaatt catacattgg agatcctacc gaaaccgctc ttatagaact tgggatccga
     1801 gccggattga acattaaaga aaccagaaaa tcctataaca ggttgtatga aattcctttt
     1861 tcttcagaaa caaaaagaat ggtggtcgta acttcggatc cagaaaacag cgacgaggca
     1921 tatgtcttcg ctaaaggcgc gccggaaata atacaagaaa tggtgcccac agaaaacgaa
     1981 attatagaaa atataaataa taattatgcc gcaaagggcc taagaagcct tggactggct
     2041 tacaaaaaaa taagcaggca cgacctggcg caagttttgg aaggaaacaa agatgacctt
     2101 tcgctcatag acgacctaga gttcatcggc attgttgcac aaaaagatcc cctacgtcct
     2161 gaagttaaag acgcattgaa catggctttg gccgcaggta taaaaacaat agttctgaca
     2221 ggagaccaca agctaaccgc tacaacaata gccaaagaac tcggccttat cagaaacgat
     2281 gacgaagtga tggacggccc cgatctcgca aaacttgacg atcaagaaat aatcaacgca
     2341 ttgaaaacag ttaaagtttt ctccagggtt tccccggaac aaaaactacg aatagttcaa
     2401 gtaataaagg gcatgggaaa cattactgcg gttacaggag acggggttaa cgacgcgcca
     2461 gccataaagg ctgccgacat aggaatttca atgggtatct ccggcacgga tgtgtcgaaa
     2521 gaagtctctg atatggtatt gcaagacgac aattacgcaa caatagttaa agcaataaaa
     2581 gaaggcaggg ttatatacga caacttaatt aaatttatat cttatttgat ctcttgcaac
     2641 atgtccgaga tatttgttgt gggtcttgcg atggtagcta aacttccact acctatgctg
     2701 ccgattcaga ttctctggat taatttaata acggacggcc tgccggcact tgcgctaggc
     2761 atggaaccgg gagaaaaaga tattatgcta agacagccta gaaaacaagg caacctactg
     2821 actaaaaaaa gatgggttcg gatgactata caaggagctt caatgactgt cggcgtattc
     2881 ggagtatttc tatttgcgtt aagaatttct cccgaggctg cgcaaagcgc agccttcacg
     2941 gcacttgcaa tgtctcaact ttttcacgca ttcaacagca gatctgagca ccattctata
     3001 ttttccagaa ctgttcccag aaacaacttt ttaatcgcca cctttgtttt atcggttttt
     3061 ttgcaattaa tgacgctata ttcctggctg ggcaacactt acctaaagac aaccccaata
     3121 cctataagtt tatttatact agcattgcta tcggcgacca taccggtagt cgtaacagag
     3181 atttataaac tattacaaaa tagagcaaag gcctaaacac aaaaaactaa ggcatagaaa
     3241 tcgggctgta aaataaacaa gtttgattct cacaccaata ttcagataca atctagtaat
     3301 ggatttattt ttcttcctct ctctagtctt aggcgcatcg gtaatgctaa taaaatcagt
     3361 gtcgtggttt acggaatctt cagccaaaat agcacaccac ttaaaaattt ccgaatacac
     3421 cataagcttt ttcttgatag caataggaac ctctttacct gaagcatttg tagcaattag
     3481 atcaggccta gcacaagatt caattttatc atttggaacc tcaatgggat caaatatagc
     3541 cctggtgacg ctagtaatag ctttgccggt attattaaca ggatcaataa gcactcgtta
     3601 tattattgct tccagagata tgtacattgc gtcgtttttg gcactattac cttttatttt
     3661 atctcttgac ggcgttatcg gaagatacga cgccataacc ttatttgcag gatacctact
     3721 ctactctata gctattctaa aacgagcgag aggggtcgag agtttaatag ataaaatcga
     3781 agaaataaat ctattaaaag aggctttctt atttattttt tctctcgcgc tactgctcgc
     3841 ggccagcaat attattgtta taagcgcaat aaacataagc gaaactctaa acataagcct
     3901 tggctttgta ggattaacaa ttacagcact tggaacatct ttaccagaaa tatcttttac
     3961 catcgcagct gtaaaaaaag gtcagacgga agaaataatg ggagatatta taggaagcgt
     4021 agttgcaaac tctaccttag tcttaggact tgctgcaatc ttctatccta ttaaaatcga
     4081 cgggataaac atgggagcaa taacaacctt ttttgttgtt ctttcctttt tgatgtttat
     4141 gaggctggca cgtactcaac aaaaagtgac aaaaaaagag gctatagttc ttctgggaat
     4201 ttacacaaca tttattttta gtgaattaat tataagactt taatcaacgc cggatccgta
     4261 aaaaaccaaa aaacattaaa atacgcagaa aaatttgatt ttttaatttg aatcggagaa
     4321 atgggctgac tgaatatagg tacttgaaat attttctaac ggagcaacaa ctacttctac
     4381 gttccttgtt ccgtcgctta tttcaactgc ttttgaaaaa ggaatactgc caagacttac
     4441 aactctataa tttatcggat cagaatgcca agacggtcga acataaaact tgatgtaaga
     4501 atagttgctc caatcatctt gggcggatac aactacttcg tgcaaatcgc tgacaccccc
     4561 acttaccaag cccattcttg ttccggcagt taaatttgaa acgcatgaat cataatcctt
     4621 acaaaggtat tcaaccaaat cccatgtgcc agactttgaa cttatctgaa cgccaaaacc
     4681 gggttctgtt gttgctttgg tattagctcc tttaactgtt tctgcggctt gctgattaat
     4741 aaaaaaatag taaacgggta ttgaaaccag tgaaaaaaca aaaattaaga tcagagggac
     4801 agaataaccc ttttgtgaat tcatagaatt atattaacct aataaaacac aaacatgcaa
     4861 caacttgttg aatattttat gcaaatcaac caccaccagc ccatgcaggt ggcaaactgt
     4921 aatttctttg cgctgcaaag atcaacatat tgggtaaact acagctacta gcttaattaa
     4981 gtagttttaa gtaacgaaat tataaattca aacggatccc tcaaaagctt ggtaatttta
     5041 agacaacctt tatgtggcgc gatcaataaa taaattgtaa tatctacata tgcagatcgg
     5101 acaaaaagat gaaaaattca gcaatgttga aggcacccca tcacaaaaaa cacaggcagc
     5161 aaaggcccca aaaattgctt ttgttcatga atatttagtg cagtatggcg gagctcaaaa
     5221 gactctagag gctatgctcg aagtatttcc caactcccct atctatacag gcctttacga
     5281 caaaaaaagc atgtcatctt ttttgaacaa ccaaacaatt cacagtacac aaaataaact
     5341 tttgtctaaa tttcaaaaat atcttacctt tctgatgccg cttgtatttg aggggatgga
     5401 tttacaagat ttcgacataa taatttcaga cgggacagct tggcctaaag gagttctaac
     5461 taacacacac cagcttcaca taagttatat tcacacgccg ccgagatttc tttatggata
     5521 ctcggttgaa agccaaaaaa gagataaatg gtactttaaa ccaatactta aagttgtcga
     5581 taacattcta agggtctggg attacaatgc ggcccaaaga cccgactttt tactaacaaa
     5641 ttcatttgaa actttagcga gaataaaaaa attttatgga cgagaggcta aggttattta
     5701 ccctccagtg gagctgtcct ataacaaccc cgaaacaagt tcggaagaaa aaattatagc
     5761 agaagagcag gaacaaaata cctcaaagaa ctattacatt atgctgggcc gcttgtctgc
     5821 atacaagaat tttgatgttg tcataaaagc tttcaacaaa aacggtaaaa accttgtaat
     5881 actgggcaca ggctacgaag aaaactatct tagaagcatc gcaaaaacaa atataacatt
     5941 catcgggcgg gccacagaca agcaaaaatc ttattatctt gaaaactgta agggccttat
     6001 aaacgctgtc caggacgaag acttcggcat cgttccaata gaggcaatgg cttacggaaa
     6061 gcccgttctt gcacataggt cgggcgggca ccttgaaaca atcgtggaag gcctatctgg
     6121 catgtttttt gaatcacttg aagtaggaga cctaataaag tcgatagaga gttttgataa
     6181 aagcatagaa gagggacttt ttgacgccgg ttcaattaaa aaaagtgtac agaagttcga
     6241 caaagaaata ttcaagaaag acctaaaaga ctttgttctt tctaaatggg aggaaaaact
     6301 aaatgccgga gctaccagaa gttcacacca tagttcaagg aatcaataaa agtttacttg
     6361 gctataaaat actcgaagta agagacatta agaaaaagta caacctccta tctcaggaag
     6421 acattgactc aaaagtaata aatgcaacta tagtatctgc ggaaagactt gcaaaacaaa
     6481 ttgttctaaa ccttgataat caacatagcc tgagcttcca tcttgcgatg acaggaaaac
     6541 tactactcag agagcccggg cacccccaag acaaagcgga aaggctcgtt ctcaacctca
     6601 tatatcaaaa tggggcccgc gagctcagat tctgcgacac acggttgttc gggaaagtaa
     6661 aaatactcca tagccaagag atagaaaaaa gtaaaaataa tttcgggctc gatcccacaa
     6721 caaaagaatt taacagtaaa aatttttaca acgtcattaa atcaaaaaat acaagtataa
     6781 aaaatctgct tatggatcag agaaaaattg caggactcgg gaatatatat gccacagacg
     6841 ctttatggtt ggcaaacata aaccccacaa ctcacacatc gcaaataacc aaagaacagg
     6901 cctcgaaact actagacagc gttatcagcg ttttaaatga gggcatcgcc cacagaggca
     6961 tatctatgag agactatgta gacctgtatg gtgaaaaagg ctcccatcag aaccacttta
     7021 aggtatacca gcaatcgcac tgcagtaggt gccagtgcga gatacaaacg gtaagaataa
     7081 acggccgggc tacgtatttt tgccctttat gccagcccca caaagacaaa acgctgaaca
     7141 aaaaaatttc ggtaaactaa tttataaaac acacaatgtt aattacaaaa ataaagctta
     7201 ctaactacag aaaccacgaa ttgctagaac ttaaattcaa cggccagtca tttattataa
     7261 aaggaccaaa cggaataggt aaaagcaata ttctcgaggc aatacacctt gtttcaacaa
     7321 ctaaatcatt aagaactaaa tacgacagag acctcattaa tcataatgct gacgtagcac
     7381 acatagaaat agtagtaaaa gatcagggca gcaaaccaca acttgccttt gaggaggaac
     7441 ttatgctttc tattcaaaaa agccagacct acccaaacgc atcaaaaaaa gttgttaaaa
     7501 taaataaagt accaaaagca ctaagagact tctccggaaa aataaaaagc gtgcttttca
     7561 caccagatga tctcgacata ctttccgggc ctccgtcaga tagaagaaaa tacatagacg
     7621 ccataatgtt tcaatctaat gaatcctata agtccgccca tttaaaatat acaaaggcca
     7681 tcaggcatag aaataagatc ctagaaaaaa tagccaaaac agggcatgga cataacttgc
     7741 ttcccgtttg gaacgaaatt attttgctaa acggagaaat aatacaaaaa aatcgcagag
     7801 aactatttga aattcttgga aaaaaagccc aagtctacct agaagaactt gaccctcaaa
     7861 actcaagatt aaaaatcaac tatctggaaa atcacatatc tgagaaacgg cttgaagaat
     7921 attcacaaaa agagatatac actcgatcaa cacttgtagg gccccaccga gacgacttcg
     7981 aggttgatta taacaactac aacagcgctt tttttagctc ccgaggacaa caaagaacaa
     8041 taatgctagc cataaaaatg gctgaaatgg accacatagc acaagctaca ggaattagac
     8101 caatactgct attagacgat atattttcgg aacttgatcc taaacacaaa gaggcagtaa
     8161 tgaacattac caatcttcaa cagacaataa ttactactgc aatagattcg aaaaacggac
     8221 tagagatagg ggccctttaa aaccaaccgc caccggctta cgcaagtgtt ttactgtaat
     8281 ttctttaagc tacaaagccc aacatgtcga gtaaactaca ccgactagct tacgcaagta
     8341 gttttgaatt ataaagccct tgcctaaccc actgaagatg cctctacaga gatgtaaaat
     8401 atatgttatt ttaactttgc ggtaaatatg cgagaattat ttagtgttct ctcctgaata
     8461 tacaattaca tcggatatac taaaaaacgt ctcgggaata gagtatgcga aggctattat
     8521 tgaaaacaca cctttgctaa gcaactggga gaatcagctg atcgtagacg caaaaacaag
     8581 aactatatcc tacgcaatac aaaaggacgg aacaaacatc aatccggaag acataaaaaa
     8641 actaataaca aacttaccta ctaaggttcc gcacatagcc gagagatacc tctcaacctt
     8701 ggaattatct cacgactatg catcaacaaa agaactaact gaggaggata ttgctgactt
     8761 atacaaggcc ttaaccggaa catcaaaata tagaaatacc ccatctcaaa aaggagtgcc
     8821 tgcagatgaa attttagctc aaatggttga gctggtagac tggctgcaaa gtgtagaagc
     8881 cctgcaaaca caccctatgt tgctggctta catagttaaa ctaaacattg aaataataca
     8941 accttttgaa aaacataatt ctctacttgc cgatctaatt tcagacgtac tgctggaatc
     9001 tcacgggtac aacattaaag gatatttctg cccacaagag tatgaataca aaacatatct
     9061 cgattatcaa gagcttattg atgatgcccg caagtcttat gacttaacac agtggcttga
     9121 atatacaacc gatattgctc atcgagagct ttctacagtg gcggataaaa ttaaaatgct
     9181 tgcaaaagat gctaaaatcg cgaaagcggc cggaagagca aagttgtcac agcgacacga
     9241 acgtataatc agctatcttc aggactacgt attaattcag aacaaggact ttcctgccct
     9301 ttttcctaat ctgtcggaag atacgatttt aagagatttg aaaaagttaa tagatatggg
     9361 cattgtagtt aaattaggca gtacaaaatc ttcgcgctac gaactctcaa attaactata
     9421 ggataagtcc gatttctata gtataatagg aagatgcagt ttggagacta ttctaagtat
     9481 ctacaaaggc tcgaaaacac ctcggctaga ctggaaatta caagcatcct ggccgaaatg
     9541 atcgagattc ttgaacctga agaaataaaa caagcagttt acctttcact tggctacctt
     9601 gatgcgccgt ttaaaagcat aaaatttaac atggccgaaa aactggcgct aagagcaatc
     9661 tctatagcct acaacatagc tgaaacagat gtagaaaatc tctataacga tttgggggac
     9721 cttggtctgg ctgcagaaaa actgtcacta aaacaccagg gacaagagcc tatcaaaaaa
     9781 cagacggtag ttgaggttta caacgctttg cttaaaattg ccaatgcctc aggaacaggc
     9841 tctcaggaca aaaaaattat tacaacagca gaattgctaa aatcgacaga ctcggtttcc
     9901 gcaaaattta taataagaat cattcttggt actaacaggc taggttttac tgaactaact
     9961 attctggacg cgttagcaaa agttcttggc aaaaacaaaa aatttggaaa aagtatagaa
    10021 gaaaaatata acacacaccc cgatatagga tatgtaacct acctcataaa aacaaagggc
    10081 gagaaaggtc taagagaact ggatattgaa atcggcatcc cgatactggc acaaaaatgc
    10141 cagagacttt cgggcgtcga agagatattg gaaaaaatgg gagaggtata cgcagagtac
    10201 aagtttgacg ggaccagagt acagcttcat atggacagaa gcgtccttaa tacagctcga
    10261 aaaagcttgt ttgaagaact taaagaggac aaaggttttt taattaaaac gtacacacgg
    10321 aacttagaag aaacaacaca ccaatatccg gagatcgtaa ctgcggcaaa caaacaaata
    10381 aaagccgaaa gtgctatatt gg
//
LOCUS       DAOSWN010000021        10132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_4190_length_10132_cov_4.188945, whole genome shotgun sequence.
ACCESSION   DAOSWN010000021 DAOSWN010000000
VERSION     DAOSWN010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10132
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4190_length_10132_cov_4.188945"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(230..508)
                     /locus_tag="PLF29_01690"
     CDS             complement(230..508)
                     /locus_tag="PLF29_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020407307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HXK52680.1"
                     /translation="MKKLYVGNLPYKMDDDALAQLFSQVGNVISAFVIKDRATGRSKG
                     FGFVEMDDADADAAILQLNDKEVEGRALKVTEAKPQEDRPRKDFRKRY"
     gene            complement(524..649)
                     /locus_tag="PLF29_01695"
     CDS             complement(524..649)
                     /locus_tag="PLF29_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52681.1"
                     /translation="MKERNHQLPLPTASQVKAIETTSKKNQVFPSGLSLELINLI"
     gene            complement(767..3244)
                     /gene="leuS"
                     /locus_tag="PLF29_01700"
     CDS             complement(767..3244)
                     /gene="leuS"
                     /locus_tag="PLF29_01700"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HXK52682.1"
                     /translation="MDKYNHKKVEDNWKKHWFDNNICKAVDFSPKPKKYILAELPYPS
                     GKFLHLGHMMRYTVPEIYSRFMRMRGYNVLFPMGWDCFGLPAETYAIKNGITPQEAVA
                     EAMTNFKASMQDMGYAIDWAREIDTSDPKYFKWTQWMFIELWKKGLAKVKVMPVWWCK
                     ELGVLADEEVLPDPDGPTGKKAERDGHPVEKRLFKQWVLDIPAYADRLLKDLDTVDYN
                     EAVKEGQRNWIGKSEGAKIIFRVKNVEEKEIQLEVFTTRPDTIFGVTFLAISPQHPAV
                     ELLIKNTKNEKQLKDYIEKASGLSDLEKQKKDKTGVEIIGVLAENPLNWRRVPIFLAD
                     YILADYGTGAIMGVPAHDGRDYEFATKYNLEVVEVINNPKNNQENNDNADSEKLLYEG
                     KGVLKNSDSFNNMGSDEATKAILQKLKEIGAGEEAVTYKIREQIFSRQRYWGEPIPLI
                     YKKDGTIENIPIEQLPLELPIIKDFYATKEGESPLEGVGEWVNTTDSQGNAAKRDTDT
                     MPTWAGSNWYYARYIDPKNDKEFANMDKLKYWMPVDKYFGDAGHTTAHLIYARFWYKF
                     LFDLGLVPSSEPFNWRMSGGILLGADNRKMSKSRPEYSVDPKDTIDNYGADATRTFLA
                     FLGPYSETFPWNENGIKACYRLMRDIYNLRVKVSNDIGSKGHAIEKDYHKTLKNITGM
                     LEDLKMNTGVSEIMKFVNLLKKQKDIPQDIWRGFIAILAPFAPFLSEQLWQEINNYAD
                     WIDENSIHLQEWPQYIEELTVDAMVNIPIQINGKLRAVIAVKSEISEAEIREAVILED
                     KVRKYITSPEDIKNFRYVKGKVVSITI"
     gene            3302..3481
                     /gene="rpmG"
                     /locus_tag="PLF29_01705"
     CDS             3302..3481
                     /gene="rpmG"
                     /locus_tag="PLF29_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002556991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HXK52683.1"
                     /translation="MAKKKKKNRMLIGLECTVTGIRSYITQKNKMNTTEKLQLMKYNP
                     KLRKHTLHKEIQKLK"
     gene            3573..4253
                     /gene="rsmI"
                     /locus_tag="PLF29_01710"
     CDS             3573..4253
                     /gene="rsmI"
                     /locus_tag="PLF29_01710"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HXK52684.1"
                     /translation="MTSSVLYIVPTPIGNMEDITIRAIDILNAVDLIVSEDTRQTQKL
                     LNHYGIDKPQISYRDQNHHKAYPKILDALIKGSAVALVSDSGMPTISDPGFKLVSSLI
                     EENIKIEALPGASAVTTAIAASGLPTDKFSFIGFLPRKETAAKKMLKTYGELDCTLIV
                     FESPYRVLKTLTLIDEALQDRKICVAKELSKIHEAYFRGTVKDVLPQLTTSNLKGEFV
                     ILISKKGF"
     gene            4253..6355
                     /locus_tag="PLF29_01715"
     CDS             4253..6355
                     /locus_tag="PLF29_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52685.1"
                     /translation="MSLDNKRDPIFEQELNKLKQVLAKLKNARKELLESLNTLGKDNL
                     KHLSDLHDSAESSDYAMFIQQLHEKNVSFNLVDKIKRQDELTYLTSEPYFARLDLKKV
                     VSGQANASSPEDAASHESIYIGKFGYNEDTPIVTDWRAPIASIFYKYRYPQKNVTYVS
                     PDGEVTRDLLLKRTFEINDGELVKYYNNDLQLDENEIISDKISKRTGGVLEDIIETIQ
                     ESQINIIESDPRQICVVQGTVGSGKSTVAIHKLAHIFFNYHGTILPERSILVTKSSVL
                     VSYLATLFPKLGIFDVNYKTLRELVVNIIFRESIGIKYDLDDEEGLADFSIEKIDELN
                     SKIQKIHNHYKAVFSELLKDESFERLTSFVYNSKMSVMENVADFVSDLEEELDFQKTY
                     LKEHPNSIKEWLYKENISNLRKLIKKAKSIKQELDSKTFPAICSDSDINPKEILGYTD
                     TLVYLYIHLKLYGFNKVLKFQYCVVDEGQDFSVLEYLLLGSVVIHGRFCILGDLNQSY
                     LEEGLESWSQIEQVIEDASRAQTFVLDTNYRSTKQIIEYANKVLSPYTSDYLPIPINR
                     PGPEVEELLFDTQDELFETFREKISSDLGMLDKSVGVIFFDDSLQAKVMEIVGDECVV
                     NKVDKKVIVRLDEKSRISYIPKGVYVSSFQVCKGLEFSKVYVLGLNIGNINNFIKAKK
                     AFVSVTRAMNEAVIMGLK"
     gene            6368..8086
                     /locus_tag="PLF29_01720"
     CDS             6368..8086
                     /locus_tag="PLF29_01720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXK52686.1"
                     /translation="MFLRLKNYILKNIDGLTVIIIAGSLRFINLGYSDFQGDEIKALF
                     LIEPGQSLSDFLLSQRKGPVQFIITYFVKILDPSYSNYFLDRFIFALFGFLAAVVFYK
                     LVKSLFNSNIAVFAALFFSTNGFLIALSRIIQYQSAVILFMLLALYSFTHAITKESRS
                     RIYIILGFVFWAISILSHYDGVFIFPFAACLLYLWWTKGREGYLFGPANKTKTIILGL
                     VLLVGVLSIFYIPFIKNIDAATISYWNGRVQGTGGKISSSIVLFRVYQPIYAIHIYAS
                     LFILGLSSTLLYWGKAGVGYFKRNFFKNKQSLSIKKALLEIVSNRDLLVTLSLIAWFG
                     AAFLFMEGAIELPGTHIYTYIIPATILAAIGMHNVLNASKYLYSVIFKLPTLRNIFDV
                     AAILVVTVLFSFLYAQSYIIYVDNSVEYPWQQKKFFIWNLHQPSAQFHLSLFGFPYSR
                     DWKGIKEFVYNPSVPPCPTPRNAPDKKPLCPDTKIKVYSGNERTSISRYSISYQKDYN
                     IAGYFIYIKNPQTFFDTKRQDKEKPGYWMNHYPPVKEIVVNGKIVAEIYYMPPFSKEE
                     LIEYGY"
     gene            complement(8066..8455)
                     /locus_tag="PLF29_01725"
     CDS             complement(8066..8455)
                     /locus_tag="PLF29_01725"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HXK52687.1"
                     /translation="MKQTLKAHNPIKHAKSFKYAFNGIFHALLNEANFRVQIVITAIA
                     VLLGIYYRISVEEWSILTLATGLLLSAEMFNTVLENIIDRLITDYDENAKIIKDLAAG
                     FVLIMAVTCAILLGLIFGSRILVTVFN"
     gene            8507..9565
                     /locus_tag="PLF29_01730"
     CDS             8507..9565
                     /locus_tag="PLF29_01730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXK52688.1"
                     /translation="MKKFLNKKTIAVILVCGLLVLGVALRGRERPVAVLEVTPEKTDV
                     IKSISASGTITSIDVADLSFASGGKITKIYVEKGDSVSKGALLAQVFNSPDYQTIQAA
                     KDSRDVAQRDLDLYIEQYNTNRDAVGGSDEYTIGVRRLQELLSRAEATYEAQAAGIQN
                     TYIYAPFEATIIDVLKEEGEIAVPGSTVIKVADLSNLVFEASIDQEDFGLLKEGHRVS
                     ITLDAYPDAGMEGVIEDIPLYADSVTNEFTLKIKVLAGKETQVLLGMSGDVDIVLAEE
                     KDVQALLFDSIFSDSGGNYVWVMDDTNKVGKKYVEIGLEGDIYTEIKTDLSDTKLVVP
                     SDNDIELKEGISVKVTER"
     gene            9562..>10132
                     /locus_tag="PLF29_01735"
     CDS             9562..>10132
                     /locus_tag="PLF29_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK52689.1"
                     /translation="MRKKVLVAKDLVKLYKVGTSEIRAVNGVSLEIDEGEFIAIVGKS
                     GSGKSTLMHMIGLLDTPTSGQIILNGSDVSFLKDNELAKIRNREIGFVFQSFNLLPRT
                     SSIDNVMLPLKYSNIDKSTWRQKAEDMLRLVELSDRKDNKSNELSGGQKQRVAIARAL
                     VTDPSIILADEPTGNLDSKTGVEIVELFKK"
BASE COUNT         3068 a         1903 c         1947 g         3214 t
ORIGIN      
        1 ctattcaaag agtctcaaaa gaactgagta taagagagaa tcgaataatg gtggggtcta
       61 ttcacgattg ccatcctttt gactacgacg acatgggcgg tgtaagaatt atattttaga
      121 acagagtaga atctatttta gcccacccgt aaatttctta tcgtgttagt tttaaacaga
      181 agaagccccg gcaaagccgg ggcttcaata ttacataatc aaatcggact tagtatcttt
      241 ttctaaagtc ttttctgggt ctgtcttctt gaggtttagc ttctgtaact ttaagagctc
      301 ttccttctac ttctttgtcg ttaagttgta gtattgcggc atctgcatcc gcatcatcca
      361 tttcaacaaa accgaagcct ttgcttctac ctgtagctct gtctttgata acaaaagcgc
      421 taataacatt accaacttgt gaaaaaagtt gtgctaaagc gtcgtcgtcc attttatatg
      481 gtaaatttcc tacgtatagt tttttcattc aaaacaccac ctatcaaatt aaattaatta
      541 attctaatga taggccggac ggaaacactt gatttttttt ggaagttgtt tctattgctt
      601 taacctgact tgcggtcggt aaaggtaatt ggtgattacg ctctttcatt agtactcgcc
      661 tattctagca ctaaattaaa atatagtgaa tactttgtta gatttgaaat tttttacata
      721 aataccgaaa gtcgagtcaa gcagaccctt ttcgaggcgt ggctttttat attgttatgc
      781 taacaacttt ccctttaaca tatcggaagt tcttgatatc ttcagggctt gtgatatatt
      841 ttctaacctt atcttccaaa atgaccgctt ctctaatctc tgcctcactt atttcggatt
      901 taacagcaat aacagccctc aactttccgt ttatctgaat cggaatattt accattgcat
      961 cgactgtaag ctcttcaatg tactgtggcc actcttgtaa atgaatgctg ttttcatcta
     1021 tccaatcagc atagttattt atctcttgcc aaagctgctc tgaaagaaac ggggcaaaag
     1081 gagcgagaat tgctataaaa cccctccaaa tgtcttgcgg aatatctttt tgctttttca
     1141 gaagatttac aaacttcatt atttcgctca ccccggtatt catttttaga tcttcaagca
     1201 tgcctgtaat atttttgaga gtcttgtggt agtctttttc tatagcgtgg cccttagagc
     1261 ctatgtcgtt tgaaactttg actctaagat tataaatatc tctcatgagc ctatagcacg
     1321 ccttgatccc attttcattc caaggaaaag tttcgctgta cgggcctaaa aaggctaaaa
     1381 aagttcgggt tgcgtcggct ccatagttat ctattgtgtc tttagggtct actgaatatt
     1441 ctggtcgtga tttcgacatt tttctattat cggcacctaa taatatgcct ccgctcattc
     1501 tccagttaaa aggttcggaa gacggcacaa gacctagatc aaataaaaac ttgtaccaga
     1561 atctcgcata gattaggtgt gcggttgtgt gtcctgcgtc gccaaaatac ttgtcaacag
     1621 gcatccagta ctttaatttg tccatgtttg caaactcctt gtcatttttc ggatcaatgt
     1681 atctagcgta ataccagttt gatcctgccc aggttggcat agtgtctgtg tctctctttg
     1741 cagcgtttcc ttgtgagtct gttgtattaa cccattcgcc cactccctct aaaggacttt
     1801 ccccctcttt tgtagcgtaa aagtctttta ttatcggcag ttctagaggc agttgttcaa
     1861 taggaatatt ttcgatagtt ccatctttct tatatatcaa gggaataggt tctccccaat
     1921 atctctgtct tgaaaatatc tgttctctta ttttataggt tacagcctct tcccctgccc
     1981 ctatttcttt gagtttttgt aatattgctt ttgtggcctc atcagagccc atgttgttaa
     2041 aagagtctga attttttaaa acccctttcc cctcgtataa taatttttcg gaatctgcat
     2101 tatcgttatt ctcttggttg tttttaggat tatttataac ttcaacaacc tcaagattgt
     2161 atttagttgc aaactcatag tcacgcccat cgtgtgcagg aacgcccatt attgcgccgg
     2221 ttccatagtc agccagtata tagtctgcta aaaatattgg caccctgcgc caatttagcg
     2281 gattttccgc tagtacgcca attatttcaa cgccagtttt atcttttttc tgtttttcca
     2341 gatcacttag gccggaagct ttttcaatat agtcttttag ttgcttttcg ttttttgtgt
     2401 tttttatcaa caattccaca gccgggtgtt gaggagagat tgctaaaaat gttacgccaa
     2461 aaatagtgtc tggtctggtt gtaaatacct cgagttgaat ttctttttct tctacatttt
     2521 tgactctaaa tataatcttt gcgccttcgc tttttcctat ccaatttctt tgaccttctt
     2581 tgacagcttc gttgtaatct acagtgtcca aatctttcag cagcctgtcc gcataggccg
     2641 gtatatctaa aacccattgc ttgaatagcc ttttttctac tggatgcccg tccctttctg
     2701 cttttttgcc ggttggtccg tccggatcag gtagtacttc ttcgtccgca agcacgccta
     2761 gctccttgca ccaccataca ggcattacct ttacttttgc tagacctttt ttccaaagct
     2821 caatgaacat ccactgtgtc catttaaagt atttggggtc acttgtgtct atttcacggg
     2881 cccaatcaat tgcatatccc atgtcctgca ttgaggcttt aaagttggtc atagcttccg
     2941 ccacagcttc ctgtggcgta atcccgtttt taatagcgta agtttcagcc ggaagaccaa
     3001 agcagtccca tcccatggga aaaagtacat tatatccgcg cattctcata aatctagagt
     3061 atatttcggg tacagtgtat ctcatcatat ggccgagatg taaaaattta ccagaaggat
     3121 agggaagttc ggctaaaatg tatttttttg gttttgggga aaaatcgaca gctttacata
     3181 tgttgttgtc aaaccagtgt tttttccagt tatcttctac ttttttgtgg ttgtacttgt
     3241 ccattgctgt tcatattagt aaatgttgcc tgtactgtca ataagtgcta tattcatagc
     3301 catggcaaaa aagaaaaaga aaaatagaat gctaattggt ctcgagtgta cagtgacagg
     3361 gattagatcc tacattactc agaagaacaa gatgaacaca accgagaagc ttcagctcat
     3421 gaagtataat cctaaactga gaaaacacac tcttcacaaa gagattcaaa agctaaaata
     3481 actcatagca tagaaattta agattttact gttgtttgaa tagggtatgg tattttcttg
     3541 atcataccgc gctactctgc tataattttc aaatgacttc gtctgttttg tatatcgttc
     3601 ccacccctat aggcaatatg gaggacatta ccataagggc tatcgatatt ttaaatgcag
     3661 tggacctgat agtctctgag gacactcgac agacgcagaa acttctcaac cattacggta
     3721 ttgataagcc tcagatatcc tatagagatc aaaatcacca caaagcatac ccaaaaattc
     3781 ttgacgcatt aattaaaggg agcgcagtag ctcttgtatc tgatagtggc atgcctacaa
     3841 tctctgatcc cggctttaag cttgtttcca gcctaataga agagaatatt aagatagagg
     3901 cactgcctgg cgcaagcgct gtaactaccg ccatagcggc ttcaggactc ccaaccgata
     3961 agttttcgtt tataggattt ttacctcgaa aagaaactgc tgcaaaaaaa atgctaaaaa
     4021 cttacggcga gctcgattgc accctcatag tgttcgaatc accctatagg gtacttaaaa
     4081 ccctaactct catagatgaa gctttgcaag atagaaaaat ttgtgtagct aaagagctgt
     4141 caaaaataca cgaggcttac tttagaggaa ctgtaaaaga tgtcctgccc cagctaacaa
     4201 ccagcaacct aaaaggagaa ttcgtaatat taatttcaaa gaaaggcttt taatgtcttt
     4261 agataacaaa cgggatccga tctttgagca agaacttaat aaacttaagc aggttcttgc
     4321 aaaactaaaa aatgcaagaa aggagcttct tgagagcctt aacactctag gcaaagataa
     4381 cctaaagcac ttaagtgact tacatgacag cgctgaatct agcgactacg caatgtttat
     4441 acagcagtta catgagaaaa atgtttcatt taatcttgtg gataaaatta aaagacaaga
     4501 cgagctgaca tatttaacaa gcgagccgta ttttgcgaga ttggatttaa agaaggttgt
     4561 gtcggggcaa gccaatgcaa gctctcccga agacgccgct tctcacgaaa gcatttacat
     4621 aggaaaattt ggctacaacg aggatacgcc gatagttaca gactggagag ctccaatagc
     4681 ctcgattttt tataagtata gataccctca aaaaaacgtt acgtatgttt ctcctgatgg
     4741 agaggtaaca cgagaccttt tgctgaagag aacttttgaa attaatgatg gtgaactcgt
     4801 taaatactac aacaacgacc ttcaattaga tgaaaatgaa ataatttcag acaaaattag
     4861 caagagaaca gggggagtcc ttgaagatat tattgaaaca atacaagaat cgcagattaa
     4921 cataatagag tcagatccta gacaaatctg cgtggttcaa ggtacggtag gaagtggaaa
     4981 aagcaccgta gcaatacata agcttgctca catatttttt aattatcatg gaacaatttt
     5041 acccgaaaga tctattttgg taacaaaaag cagtgttctt gtaagctacc tggcaacgct
     5101 gtttcctaag ctaggtattt ttgatgtgaa ctataagacc cttcgagagt tggttgttaa
     5161 tattattttt agagaatcaa ttggaatcaa atacgattta gatgatgagg agggtttagc
     5221 agactttagt atagaaaaga tagacgagct aaactctaaa atacaaaaga tccacaatca
     5281 ttacaaagct gtcttctcag aactattaaa agatgagtct ttcgagcgtc tcacgagctt
     5341 tgtttataac tcaaaaatgt ctgtcatgga aaatgtggct gattttgtat ctgatctgga
     5401 agaggagctg gatttccaaa agacttatct aaaagaacac ccgaattcaa taaaagaatg
     5461 gctttataag gaaaacatta gtaatttaag aaaactaata aaaaaggcca aatccattaa
     5521 acaggagcta gattctaaaa ctttcccggc tatatgttcc gattctgata taaacccgaa
     5581 agaaatctta ggatacacag atacgctggt ttatttatac atacacttaa agctttacgg
     5641 atttaataaa gttttgaaat tccagtattg tgttgttgat gaggggcaag attttagtgt
     5701 tctagaatat ttacttcttg gatcagtagt tattcatgga agattttgta ttttaggaga
     5761 tttaaatcaa agttatttag aagagggttt ggaatcttgg tcacagattg agcaagttat
     5821 tgaagatgca agtcgtgctc aaacttttgt tttagacaca aattatcgaa gtactaagca
     5881 aattatagaa tacgccaaca aggttttatc tccttatacg agcgattacc taccgatacc
     5941 aataaatcgg ccgggccctg aagtcgagga attacttttc gatacccagg acgaattatt
     6001 tgaaacattt agagaaaaaa tttcatcaga ccttggtatg ttagacaaga gtgttggggt
     6061 aatatttttt gatgactcac ttcaggcgaa agtcatggaa attgtagggg atgagtgtgt
     6121 tgtaaataaa gttgataaga aagttatcgt tcgcctcgac gagaagtcca gaatcagcta
     6181 tatacctaaa ggggtttacg tatcaagctt tcaggtttgt aagggactgg aattctcaaa
     6241 ggtttatgtg ctaggcttga acataggtaa tattaacaac tttataaaag caaaaaaagc
     6301 tttcgtttcg gtaactcgag ccatgaacga ggctgtgatc atgggtttaa aataaaaaac
     6361 taaaagaatg ttcttaagac taaaaaacta catattaaaa aatattgatg ggcttacggt
     6421 tattattatt gccggaagcc ttagatttat caatctcggt tattccgatt ttcaaggtga
     6481 cgagatcaag gctttattct taatagagcc cggacaaagc ctcagtgatt ttttgctgtc
     6541 ccagcgcaaa gggccggttc agtttataat tacttatttt gtaaagatac ttgatccgtc
     6601 ttactcaaac tattttcttg acagatttat tttcgcacta tttggatttc tggctgctgt
     6661 ggtgttttat aaacttgtaa aatcactctt taattcaaat atagctgtct ttgccgcctt
     6721 atttttttca acaaacggtt ttttaattgc tctttcgaga attattcaat atcagtctgc
     6781 tgtcatactt tttatgctat tggcgcttta ttcttttacg catgctatta caaaagaatc
     6841 acgaagccgc atatatatta ttttaggctt tgtgttttgg gcaatatcga tactatccca
     6901 ctatgatggt gtttttattt ttccttttgc cgcctgcctt ctttacttgt ggtggacaaa
     6961 gggtagggaa ggctatctat ttggtcctgc aaacaaaaca aaaactataa tactcggctt
     7021 ggttttgcta gttggggttt tatcaatttt ttacatacct tttataaaaa atattgatgc
     7081 tgcaaccatc tcttattgga acggcagggt gcagggaacc ggaggaaaga tctctagctc
     7141 tatcgtactt tttagagttt atcagcccat atacgcaata cacatttatg cttcgctatt
     7201 tattctcggt ttgagctcaa ctctattata ttggggtaaa gctggggtcg ggtattttaa
     7261 gaggaacttt ttcaagaaca aacaaagcct ttcaataaag aaagcgcttc tcgaaattgt
     7321 ctctaatcgt gatttactag taaccctttc tttaatagct tggtttgggg cagccttctt
     7381 attcatggaa ggagctatag agcttcccgg cactcacata tacacatata taatacctgc
     7441 aacaatctta gctgcgatcg gaatgcataa tgttcttaat gcttctaaat acctatacag
     7501 cgttattttc aaacttccaa cattgagaaa tatttttgac gtagcggcta ttctagtagt
     7561 aaccgtttta ttctcttttt tatatgccca gtcatacata atttatgttg ataactccgt
     7621 ggagtaccct tggcaacaaa aaaagttttt catttggaat ctccatcaac ctagtgcaca
     7681 atttcacctt agcctttttg gttttccgta ttctcgtgac tggaaaggaa tcaaagaatt
     7741 cgtttacaac ccttcggtgc caccttgccc aactccaaga aatgctcctg acaaaaaacc
     7801 gctctgtccc gataccaaga taaaagtata ttcaggaaac gagcgtactt ccatatcgcg
     7861 atacagcatc tcttaccaaa aagactataa tattgccggc tattttatat acattaaaaa
     7921 cccacaaaca ttttttgata ctaaaagaca agataaagag aagccgggtt actggatgaa
     7981 ccactatcct cctgttaagg aaatagttgt aaacggaaaa atcgtggcag agatttatta
     8041 tatgccgcca ttctcaaaag aagaactaat tgaatacggt tactagaatt cttgatccga
     8101 aaattaaccc aagcaatatt gcgcacgtaa cagccattat taaaacaaat cctgctgcta
     8161 gatccttaat tatttttgca ttttcgtcat agtctgttat tagcctgtct attatgtttt
     8221 ccaaaacggt attaaacatt tctgcggaca gtagcaagcc ggttgctaaa gttaaaatag
     8281 accattcttc aacggaaatt ctgtaatata ttcctagcag cacagctatt gcggtaatta
     8341 caatctgtac tctaaagttt gcttcattta aaagcgcgtg gaatattcca ttaaatgcat
     8401 atttaaagct ttttgcgtgt tttatcggat tatgtgcttt taaggtttgt ttcatagtat
     8461 aatgatagac tcggtacaaa atttgtaaag tgatatactt tttaagatga aaaaatttct
     8521 aaataaaaaa acaatagctg taattttggt gtgtgggctt ttagtgcttg gtgttgctct
     8581 aaggggcaga gagcgtcctg tggcagttct tgaggtcacg cccgagaaaa cagacgttat
     8641 taagagcatt tccgctagcg gtacaataac ctctattgat gttgcagatc tctcttttgc
     8701 ttcaggtggc aaaataacaa aaatatacgt agaaaagggc gactctgttt ctaagggggc
     8761 tctcctcgct caggttttta attctccaga ttatcaaaca atccaagccg caaaagattc
     8821 tagggatgtt gctcaaagag atcttgattt atatattgag caatacaata caaacagaga
     8881 tgcggtggga ggctctgacg aatacactat aggtgttaga aggcttcaag agcttttaag
     8941 cagagctgag gcaacatatg aggcacaagc cgcaggtatt cagaatactt atatctacgc
     9001 gccttttgag gcaacaatca ttgatgtgct aaaggaagaa ggtgagatag ctgttcccgg
     9061 atctacagtt attaaggtgg ctgacttgtc gaatcttgtt tttgaagcga gtattgatca
     9121 ggaagacttc ggattactta aagaaggcca cagagtttca ataacactcg atgcataccc
     9181 tgacgccgga atggagggcg taatagaaga tataccttta tatgctgatt ctgttacaaa
     9241 tgagttcaca ttgaaaataa aggtattagc cggcaaagaa acgcaagtac tcttaggcat
     9301 gtccggagac gtagatatag tgttagctga ggaaaaagat gtccaagcat tgctttttga
     9361 ctccatattt agcgattctg gaggaaatta tgtatgggta atggacgata ctaacaaggt
     9421 aggtaagaaa tacgttgaga tcggattaga gggcgatatt tacacagaga taaaaactga
     9481 cttaagcgat actaagcttg tagtaccaag tgataatgac atagagctta aagaaggtat
     9541 aagtgttaag gttacggaga gatgagaaag aaagtacttg tagcaaaaga ccttgttaag
     9601 ctgtacaaag ttggcactag cgaaataagg gctgtaaacg gggtctctct agaaatagat
     9661 gaaggggagt ttatagccat agtaggaaag tccggctccg gaaaatccac tttgatgcac
     9721 atgataggtt tgctagatac tcctacctca gggcagatta tattaaacgg atctgacgta
     9781 agtttcctaa aagacaacga attggcgaaa atcagaaatc gagaaatagg ttttgttttt
     9841 caatctttta atctgcttcc gcgtacttcg tcaatagaca atgttatgtt gcccctaaaa
     9901 tacagtaata ttgataaatc aacttggaga cagaaggcag aagacatgtt gaggctggtt
     9961 gaacttagcg accgcaagga taataagtca aatgaacttt ccggaggaca gaagcaaagg
    10021 gttgctatag caagggcgct tgttactgat ccttctataa tattagcgga cgaaccgaca
    10081 gggaacctag attcaaaaac aggggttgag attgttgagc ttttcaaaaa ac
//
LOCUS       DAOSWN010000022         9309 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_4720_length_9309_cov_5.163929, whole genome shotgun sequence.
ACCESSION   DAOSWN010000022 DAOSWN010000000
VERSION     DAOSWN010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9309
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4720_length_9309_cov_5.163929"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="PLF29_01740"
     CDS             <1..446
                     /locus_tag="PLF29_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXK52690.1"
                     /translation="STWAPSPEKYEAGTPNVAGAYGLGIAVDFINSIGMDSIVSHGEE
                     ITGYAIDRLSSIDGMFIYGPKVAAERGALVSFSLEGLHSHDVAAVLNAEGIAVRSGQH
                     CTMPLHDKLQVNSTIRASFGIYTTKEDIDALCDAILKAIKLLRGA"
     gene            481..861
                     /locus_tag="PLF29_01745"
     CDS             481..861
                     /locus_tag="PLF29_01745"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HXK52691.1"
                     /translation="MADIYTREDLLEIYKNPMHRAKIKSPTVSTSVRNEMCGDSLDLE
                     LQFAEGKLIDAGFTGDSCAVSIIAASLVLDFLIGRSISEIRSFTKNDILDLLSIDLTT
                     SRVKCATLILDALQVALQSYDKKD"
     gene            845..1165
                     /locus_tag="PLF29_01750"
     CDS             845..1165
                     /locus_tag="PLF29_01750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HXK52692.1"
                     /translation="MTKKIKQNKNSIKPAPADKVGKDVMANTADTSTLSHSKVTKETN
                     LGELVMEYPEAAEVLLDYGLHCVGCFANTFDTIEMGAKIHGMGDEEIAELIERVNEVI
                     NFEE"
     gene            1167..1475
                     /locus_tag="PLF29_01755"
     CDS             1167..1475
                     /locus_tag="PLF29_01755"
                     /inference="COORDINATES: protein motif:HMM:NF018595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HXK52693.1"
                     /translation="MSKKVPDAQKNKNLVLGKDVSSDPKTKLIHRIEVVRELCIGATT
                     CVVVSPQAFEMDDDSIAVVKENGEKISDDELLMAAQSCPTAAIILYDKDGNKIFPKDN
                     "
     gene            1541..1897
                     /locus_tag="PLF29_01760"
     CDS             1541..1897
                     /locus_tag="PLF29_01760"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HXK52694.1"
                     /translation="MKYQITSDNMQVTASMQNLVMSKMGKLEKLWGRIEEDLTNIRVV
                     LNSAPGADTKFEVKIEANVKGEIYYADGIGYSFEEALVSAVNKVERSVLKDTDRSEKW
                     DRVRDAKVVSEDDLVL"
     gene            2048..2123
                     /locus_tag="PLF29_01765"
     tRNA            2048..2123
                     /locus_tag="PLF29_01765"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2081..2083,aa:Ile,seq:tat)
     gene            complement(2195..3169)
                     /locus_tag="PLF29_01770"
     CDS             complement(2195..3169)
                     /locus_tag="PLF29_01770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXK52695.1"
                     /translation="MTKKENKFISIVIPCRNEEGNMVENIRGISKVLTEAQYRHEIIC
                     IDDGSKDKTWEKIQELSKKYTELKGIRFMRNHGMTQAYSAGFDSSKGDYVITMSADME
                     IPAKNLLKVIELLDEGYDFVNTNREGRWGNADRALPSKIANNIIGKISGVYMKDTGSG
                     MKGFRRVLIDNFSLYGEMHRMIPAYLSSFGAKMTEFDVEFKDRTYGTSSYGGLSRTFK
                     VLLDIITMAFMINLTKKPFMAIPGRLFGATGALISGMGGLIAFYLLILKLMGQSIGGR
                     PLFFVSIFMIVIGLQSLMIGMIGELLMRTYFEASGRKTYTIREKINIA"
     gene            complement(3216..4190)
                     /locus_tag="PLF29_01775"
     CDS             complement(3216..4190)
                     /locus_tag="PLF29_01775"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXK52696.1"
                     /translation="MKILVTGAAGFIGSNLCEFLLSKGHQVIGLDNFNDYYSPKVKRF
                     NIESFEKNANFQLAEIDILNVEGLESMFTTHRPEAIVHLAGWAGVTYSVSNPKIYLEV
                     NIVGTNNLAQMATKHGVNSFVFASTSSIYGNTNPVPFNESMDSSKPASPYPASKKAAE
                     VLLWTYKTNFDLNVTIFRFFNPLGPKLRPDMALPKLVKAAEYGLEFPLYMLNIQTAAR
                     DYTHIVPMLETMEYVCENPLSYEILNLGNSQPIELANLIQMVERVTGKSIKIVKKPLP
                     GQMEVTYANVEAAKKIANYNPRFSAEQAVEEYYQWFLKQPEWYKKGEY"
     gene            complement(4292..4618)
                     /locus_tag="PLF29_01780"
     CDS             complement(4292..4618)
                     /locus_tag="PLF29_01780"
                     /inference="COORDINATES: protein motif:HMM:NF013012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52697.1"
                     /translation="MEKESQKLTLIINASDRYSKKITLWSETGSIDEIEGDIDIVFEM
                     HNILIRNGLDISDIIEIKSFPGPGSFTGLKIGAVIANVLNWALNKKNLEQLEYPAYGS
                     EPNIQK"
     gene            complement(4581..5015)
                     /gene="tsaE"
                     /locus_tag="PLF29_01785"
     CDS             complement(4581..5015)
                     /gene="tsaE"
                     /locus_tag="PLF29_01785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HXK52698.1"
                     /translation="MEYNITEISQTQKLAEKIAKEISENTVILLFGDLGSGKTTFSRF
                     FSIAMGSENRVQSPTFVIHRQYIAPNNENVQRINHYDLYRITNKAELEDLSIYEALNE
                     PEQVSLVEWPELLEKTQITAKIISIEFEDNNGKRIAKINTNN"
     gene            complement(4961..5338)
                     /locus_tag="PLF29_01790"
     CDS             complement(4961..5338)
                     /locus_tag="PLF29_01790"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HXK52699.1"
                     /translation="MKKNKKLLRWLPSVIVATIIFAASTIPGDQIQASGYGQEKYQIN
                     AHFVLYMALAGAVYYASQNPKRTVLICFVYALSDEFHQVFTPQRSPQIFDIAVDMVGV
                     IISTLLWSTILPKFRKHKNLPKK"
     gene            complement(5372..5857)
                     /locus_tag="PLF29_01795"
     CDS             complement(5372..5857)
                     /locus_tag="PLF29_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52700.1"
                     /translation="MGIFTATKILKFFVYLATSFCLLKANVAGARTLNFYIKSKFFEN
                     STPIILSTDSGKVLDDRVILYNLSTSNGTVEYYLEKEKRWIFPAIDEVKLNEKGEIKL
                     RLNVGNSFSTIVTITVVDKRTGANLGTTQFKIYNKKYAMYYIQRLSSYLEKQKETIKN
                     N"
     gene            complement(5982..7193)
                     /locus_tag="PLF29_01800"
     CDS             complement(5982..7193)
                     /locus_tag="PLF29_01800"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HXK52701.1"
                     /translation="MRITKILLSTTFLLGALFFIGKTSILAQLEEGPIYGATEGGDDF
                     DKTMKELEKKKQEYEKKIKDLQQEENSLQKQIDTATTQIYLTQTEINIKQQEIAETEK
                     EIRELGEDISDLKNRIAKLKKTIAIQQDYLSERMRARYKTGENSYVIMVFGSDKFNSL
                     LQKTKFLKVMEYQDKKLIAEMDATKAAYNDQKDIAEDKKKEAETLQDQLERQQNELAA
                     ARAELDRQKADKETLLRETQNDEQKYQSLLAQVESEMAALNMAINLPEGGEEVKKGDI
                     IGLMGNTGCSSGPHVHFGFIKNSKAVDPLPYLNDGHLKWPVSDWQITQGFGANYWFYM
                     NNFGIPGHDALDIVSRGQWSGAPIKAARDGKLRYAQDARVYCPWLNNSIGKGAIIDHG
                     DGEKTIYWHLQ"
     gene            complement(7196..8065)
                     /locus_tag="PLF29_01805"
     CDS             complement(7196..8065)
                     /locus_tag="PLF29_01805"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HXK52702.1"
                     /translation="MKNSFYKKEKLLIATNIISVTITFLLLGGLAFVIAFTQTGIRKL
                     EEQAQITIFFEDAYPESSILNLANELENDPQVSNVNYVSKEEAYSIFTDLNKDEPVLL
                     ESITPDILPASLEIKTKKLADMNDISERLSNMDGVEEIKFYKDVIERFTFWTNVSYAV
                     GIALVSLFLIVSYAIILITLRINIGYIGKELEILKLVGASDAYIKNPLVKQGVSLGLI
                     SAFISGALLLSFALIVQFAHLINTNIKFGFIPEIEIALPIFALIINFVLVASGAALGY
                     IGSVSAIQKYINH"
     gene            complement(8055..8741)
                     /gene="ftsE"
                     /locus_tag="PLF29_01810"
     CDS             complement(8055..8741)
                     /gene="ftsE"
                     /locus_tag="PLF29_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008340404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HXK52703.1"
                     /translation="MSQIEFFNLTKQYENGFYAVKDVSFAVSKGEFVFIVGSSGAGKS
                     TLVRCLIREEEPTAGQILFNGKNVIEIPKKELPELRRQIGVVFQDFKVLDTRNVADNV
                     AVALEVVNTPAEVKNEIIPNVLNMVGLQDKSNLFPYQLSGGEKQRLSIARALAHEPDV
                     LVADEPTGMIDPKATWEVMEILEKINTLGTTILMATHDQSIVDKLRKRVVRIEKGQIV
                     SDVEKGKYEE"
     gene            complement(8743..9159)
                     /locus_tag="PLF29_01815"
     CDS             complement(8743..9159)
                     /locus_tag="PLF29_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52704.1"
                     /translation="MLAQLKTIYSRIEALKGPKLLILLFGIFLLFFATGGLLNYFIDK
                     KLINDELLVSGQPEPQKVSISYTGKIVFAGELSMPDEKIKYKLLDEDNNDIIYLYAKD
                     QKLEVSEGLYATVEGTIFKTQDGQDVLEVYKITLKR"
BASE COUNT         2529 a         1785 c         1802 g         3193 t
ORIGIN      
        1 cttctacttg ggccccctct cccgaaaaat atgaagctgg aaccccaaat gttgccggtg
       61 cctatggttt aggtattgct gtagatttca tcaattctat tggtatggat tctattgttt
      121 cgcatggtga agagattacc gggtacgcta ttgataggct gagttcgatt gacggcatgt
      181 tcatatatgg tccgaaagta gcagctgagc gcggggccct ggtttctttc tctcttgaag
      241 gtttacattc tcacgatgtt gcggcggtgt taaatgcgga gggtatagcg gtaagatccg
      301 gccagcactg caccatgcct ttgcacgata agcttcaggt aaattcaaca ataagagcaa
      361 gctttggaat atatacaaca aaggaagaca ttgacgcgct gtgcgacgcc atattaaaag
      421 ctataaaatt acttagggga gcttagttac ttaattttac tgtttaaatt catataaact
      481 atggcagaca tatacaccag agaagattta ttagagattt ataaaaatcc tatgcacagg
      541 gcaaagataa aaagccctac cgtaagtaca agtgttcgta atgaaatgtg cggagatagt
      601 cttgatctcg aattacaatt tgcagagggc aagcttatag acgccggttt taccggagac
      661 tcctgcgcgg ttagtattat agccgcttct ttagtactgg attttttaat aggaaggtcg
      721 atatctgaga ttcgaagctt tactaagaat gatatactgg atttattgtc gatagattta
      781 actaccagta gggttaaatg tgcgactttg atacttgatg ccctgcaggt cgcattacaa
      841 tcatatgaca aaaaagatta aacaaaataa aaatagtata aaaccagctc ctgcagataa
      901 ggtgggaaaa gatgttatgg ctaatactgc ggatacgtcg acgctatccc actctaaggt
      961 aactaaagaa acaaatcttg gagagcttgt tatggagtac cccgaggctg cagaggtttt
     1021 gctggattac gggttacact gtgtagggtg ctttgctaat acctttgaca caatagagat
     1081 gggcgcaaag atacacggga tgggcgacga agagatcgct gaattaatag aaagagtaaa
     1141 tgaagttata aattttgaag agtgatatgt cgaaaaaagt acccgatgct caaaaaaata
     1201 aaaatctagt tttgggcaaa gatgtttcat cggatccaaa gacaaaatta atacatcgaa
     1261 ttgaggttgt gagagagctt tgcattgggg cgacaacttg tgtagtggtt tcaccccagg
     1321 cttttgagat ggacgatgat agcatagcag ttgttaaaga aaacggagag aaaataagcg
     1381 atgatgaatt attaatggct gctcaatctt gtcccactgc cgctattatt ttatacgata
     1441 aagatggtaa taaaatattc cccaaagaca actgacataa aaaattataa ggtcagtgaa
     1501 cttagccccc agcgagtcct taaaatggta aaatataaat atgaaatatc aaattacgtc
     1561 agataatatg caggtgactg caagtatgca aaatttggta atgtccaaaa tgggcaaact
     1621 cgagaagttg tggggaagaa tagaggaaga tctaactaat ataagggtag ttttaaatag
     1681 tgccccggga gctgacacga aatttgaggt caagatcgaa gccaacgtta aaggtgagat
     1741 atattatgcc gacggaattg gctatagctt tgaagaagcc ttggtgtctg cagtaaataa
     1801 agttgaacgt agtgtgctaa aagatactga caggtctgaa aaatgggatc gcgttagaga
     1861 tgcgaaagta gtatcggaag acgatctggt tctttagaat agtttaaggt tgttggcgcc
     1921 ttttcggcat ttacaggtgg tcaacttttt aggtcggctg ttaaattcta gatacgttaa
     1981 gggtttgtgt ccggcatttg aaaaggcgcc tttttttgct atactaggtc actaactttt
     2041 agtatatggg tgattagctc agttggtaga gcgctcgtct tatacacgag tggtccctgg
     2101 ttcgagccca ggatcaccca ccaccttcct ttattaccac gagattggca ctgagtatga
     2161 aagtaaatgt ttcccgttgt gtgcttttgc ttacttaggc tatgtttatt ttttccctga
     2221 tagtgtaggt ttttctacct gaggcctcga agtaagttct catgagcagt tctcctatca
     2281 tgccgatcat taaagattgt aaacctatta ctatcatgaa tatagataca aaaaataacg
     2341 gcctcccccc tatactttgt cccataagct ttagtataag aagataaaaa gctatgagac
     2401 cgcccattcc cgaaatcaac gccccggttg ctccgaataa tcttcccggg attgccatga
     2461 aaggcttttt agttagattt atcataaatg ccattgttat aatgtccaac aaaaccttaa
     2521 aagttctgct tagcccaccg taggaagatg ttccgtatgt acgatctttg aactcaacat
     2581 cgaattcagt cattttagcc ccaaaagaac ttaaataagc cgggatcatc ctgtgcatct
     2641 caccgtataa agaaaagtta tctataagca ctcttctaaa acccttcatt cccgaaccgg
     2701 tatctttcat gtaaactcct gagatttttc caataatatt gttagcaatt ttgcttggga
     2761 gcgccctgtc ggcatttccc caccttcctt cgcggttagt gttaacaaag tcgtacccct
     2821 cgtctagtag ttcaatcact tttaataggt tttttgctgg tatctccatg tcggcggaca
     2881 tagtgattac gtaatctcct ttagaggaat cgaagccggc ggagtaagcc tgcgtcattc
     2941 cgtggtttct catgaatctt atgcccttga gctcggtata tttcttagag agttcttgta
     3001 ttttttccca tgttttatct ttactcccgt cgtcaataca gatgatctcg tgtctgtatt
     3061 gggcttcagt tagaactttt gaaatacccc ttatattctc caccatgtta ccttcttcat
     3121 ttctgcaagg tataactatt gatataaatt tattttcctt ttttgtcata tttaattctt
     3181 cgagttatgc gtgccttgag gcaagcttgc tccgatcagt attctccttt cttgtaccat
     3241 tcaggttgtt ttaaaaacca ttgataatat tcttcaacag cttgttcagc agaaaatctt
     3301 ggattgtagt ttgcaatttt ctttgcggct tctacgttag catatgtaac ttccatttgt
     3361 cccggaagag gtttttttac tatcttaata gattttcctg ttactcgctc taccatttga
     3421 attagattgg caagttctat cggttgagaa tttcctaaat ttagaatctc ataagataaa
     3481 ggattctcac atacatattc cattgtttcc agcattggaa ctatgtgagt atagtctcta
     3541 gcagctgttt gaatatttaa catatataat ggaaattcaa gtccgtattc tgcggctttt
     3601 acaagtttag gcagcgccat gtcgggcctt aattttggtc caagaggatt aaaaaatcta
     3661 aatattgtta catttaggtc aaagtttgtt ttatatgtcc aaagaagaac ttctgcagct
     3721 tttttagatg ctgggtaagg agacgcaggt ttgcttgagt ccatggattc gttgaaaggt
     3781 accggatttg tgtttccgta tattgatgac gttgaagcaa aaacaaaact gtttactccg
     3841 tgttttgtgg ccatttgcgc taggttgttt gttccgacta tatttacttc aagataaatt
     3901 tttggatttg atacagagta agttactcct gcccaacctg ctagatggac tattgcttca
     3961 ggtctatgag tcgtgaacat gctctcaagg ccctcaacat ttagtatatc tatttcagca
     4021 agttgaaaat ttgcattttt ttcaaatgac tctatattga atcttttgac ctttgggctg
     4081 tagtaatcgt taaaattgtc cagccctata acctggtggc cttttgaaag taaaaactcg
     4141 cataagttgg aacctataaa tcctgccgcc cctgttacca aaatcttcat acgtatattt
     4201 tacagtattt tatagtgtgc gtaaattgcc gctctagaag agaaagccgc ggggtagttg
     4261 taagaaccgt tatttgttag tgcggaagtt actatttctg aatatttggc tcactgccat
     4321 aagcaggata ttctagctgt tctagatttt tcttatttaa agcccagtta agaacgtttg
     4381 ctataactgc ccctatcttc aagccggtaa aggacccggg tccgggaaag ctctttatct
     4441 cgatgatgtc agaaatatct aaaccgtttc taataagtat gttatgcatt tcaaagacga
     4501 tgtctatatc tccttctatt tcatctatac ttccggtttc tgaccacagc gttatttttt
     4561 tgctgtatct atcggaggcg ttaattatta gtgttaattt ttgcgattct ttttccatta
     4621 ttatcttcaa attctatact tattatttta gctgtaattt gtgttttttc taataattcc
     4681 ggccattcaa ctaaacttac ttgctccggt tcgttaagcg cttcgtagat gctcaaatcc
     4741 tccagttctg ctttatttgt tattctatac aagtcgtagt ggtttattct ttgaacattt
     4801 tcgttattcg gcgcaatgta ttggcgatgt attacaaaag tggggctttg aaccctgttt
     4861 tcgcttccca tggctataga gaaaaatcta ctaaaggttg ttttaccgga tccaaggtct
     4921 ccgaaaagta aaattacggt attttcgctg atttctttgg ctattttttc ggcaagtttt
     4981 tgtgtttgcg aaatttcggt aatattgtac tccatagcaa tgtagatatt ataacaccga
     5041 ccatgtctac agctatgtca aagatctgcg gagatctctg tggcgtaaat acctgatgaa
     5101 attcatcaga tagtgcataa acaaaacaaa tcagcactgt tctttttgga ttttgtgatg
     5161 cataataaac tgccccggct aatgccatgt ataagacgaa atgggcgttt atttggtact
     5221 tttcttgccc ataccccgaa gcttgtatct gatctccggg tatggtagat gccgcaaaaa
     5281 ttattgttgc aactattact gagggcaacc atcgtagtag ttttttattt tttttcacct
     5341 agtaatttta cacgatattt accttggtgt tctagttatt ttttatcgtt tctttttgtt
     5401 tctctaagta cgagctcagc cgttggatat agtacatagc atatttctta ttataaattt
     5461 taaattgtgt agtacctaga ttggcgcctg ttcttttgtc cactactgtt attgtcacta
     5521 tagtactaaa ggaattgccc acattaagcc gtagctttat ctcgcctttc tcgtttaatt
     5581 tcacttcatc tatcgcagga aatatccatc tcttttcttt ctccaagtaa tattctacag
     5641 tcccgtttga ggtgcttagg ttgtatagaa ttactctgtc atctaagacc ttgccagagt
     5701 ctgtgctcag tataataggt gtactatttt caaaaaattt tgatttgata taaaaattta
     5761 aagttcgggc accggccacg tttgctttta gtagacaaaa agaggtagcg agatatacaa
     5821 aaaattttag aatctttgtt gctgtgaaaa tacccacgat caaattctat attgctaata
     5881 taaaactttg cgcaaaagtg tctattacaa gtaggataga tttatgcctt tttgattttg
     5941 gccttggcat tacatttttt cgccaataaa gaattaagat tttactgcag gtgccagtag
     6001 atagttttct ctccgtctcc gtggtcaata atcgcgccct tgccgatgga gttgtttagc
     6061 cacgggcagt agactcttgc atcttgagca tagcgtaact ttccatcacg ggccgcctta
     6121 attggcgcgc ctgaccactg acctcttgag acaatatcta aagcatcgtg tccgggaatc
     6181 ccaaagttgt tcatgtagaa ccagtaattt gcgccaaacc cttgggttat ctgccagtca
     6241 ctgacaggcc actttagatg gccgtcgtta aggtagggga gaggatcgac cgctttgctg
     6301 tttttaataa agccaaaatg tacgtgcggt ccggaggaac atcccgtatt tcccattaaa
     6361 cctattatgt cgcctttttt cacctcttct cccccctcag ggaggtttat agccatattg
     6421 agggctgcca tttccgattc tacttgtgcg agcaaggatt ggtatttttg ctcgtcattt
     6481 tgagtttctc ggagcaaagt ttctttatct gccttttgcc tgtcaagctc cgctcgggcc
     6541 gctgcaagtt cgttttgctg cctttccagc tgatcttgta gtgtttcggc ttctttcttt
     6601 ttgtcttcgg caatatcttt ttggtcgttg taggcggctt ttgtggcgtc catttcagca
     6661 ataagctttt tatcttggta ttccattact tttaagaatt ttgttttttg taaaagtgaa
     6721 ttgaacttat ctgacccaaa aaccattatt acatatgaat tttctcccgt tttatatctt
     6781 gcgcgcattc tctctgataa gtaatcttgt tgtatcgcta tagttttctt aagctttgct
     6841 attctattct taaggtcgct tatatcttcc cctaactccc gtatctcttt ttcggtttct
     6901 gcaatctctt gttgtttaat attaatttca gtttgtgtta aatatatttg tgtggttgct
     6961 gtatcaatct gtttctgcaa agaattttct tcctgctgga ggtctttaat tttcttttca
     7021 tattcctgct tctttttttc cagctctttc attgttttgt cgaaatcgtc gccaccctct
     7081 gttgctccat agataggccc ttcttctaat tgcgctaaaa tagaagtttt tcctataaaa
     7141 aatagcgcgc ccagaagaaa agttgtcgat agtagtattt ttgttatcct catttttagt
     7201 ggtttatata tttttgaatt gctgaaacgc taccaatgta gcctagtgca gcacctgaag
     7261 caacaagtac aaaatttata attagtgcga aaatcggcaa agctatttct atttcaggaa
     7321 taaagccgaa tttaatgttt gtgttaataa gatgagcaaa ttgaacgatc agggcaaagg
     7381 aaagtagcaa tgctcccgaa ataaatgctg aaattaatcc cagactcacc ccctgcttaa
     7441 ctagaggatt ttttatataa gcatctgaag caccaactag ctttagaatt tcaagttctt
     7501 tgccgatgta ccctatgttt atgcgcagcg ttatgagaat aattgcatac gaaactatca
     7561 agaacaatga cacgagggct attcccacag cataggaaac attggtccaa aaagtaaagc
     7621 gttcaatcac atctttgtaa aattttattt cttcaacccc atccatgttc gatagtctct
     7681 ctgagatatc gttcatatcg gcaagttttt tggtttttat ctcaaggctg gccggaagta
     7741 tgtccggagt tattgattct aaaagaacag gctcgtcctt gtttaaatcg gtaaatatgc
     7801 tataggcctc ttcttttgaa acgtagttca cgttacttac ttgagggtcg ttttctagct
     7861 cgtttgcaag gttgagtatt gatgactcag gataggcatc ttcaaaaaaa atagttattt
     7921 gtgcttgttc ttcaagcttt cttattcctg tttgtgtgaa agctattaca aaggcaagcc
     7981 ccccaagcaa taaaaaggtt atagttacag atattatgtt tgttgcaatt agcagtttct
     8041 cttttttgta aaagctattc ttcatatttg cctttctcta cgtcagaaac tatctggcct
     8101 ttttcaattc tgacaacccg ttttctcaac ttatccacta tactttgatc gtgggtagcc
     8161 attaaaatag ttgttcccag cgtgttaatt ttttccaaaa tttccataac ttcccatgta
     8221 gctttggggt ctatcatgcc ggtgggttcg tctgctacta gcacatcagg ctcgtgcgcc
     8281 agtgcccttg caatagacag gcgctgtttt tcgcctcccg aaagctggta tggaaacaga
     8341 ttagatttat cttgaagccc taccatgttt aatacgtttg gtattatttc gttttttact
     8401 tcggcaggtg tgtttacaac ctctagtgca actgctacgt tgtcggcaac atttcttgta
     8461 tccaagactt taaagtcctg gaaaactaca cctatttgtc ttcttagttc cggtagctct
     8521 tttttaggta tttctatcac gttttttccg ttaaaaagta tttgccccgc cgtaggctcc
     8581 tcttctctta tgagacatct caccagagtt gatttaccgg ctccggaaga tcctactata
     8641 aatacaaatt cacctttaga tactgcgaag gacacgtctt tgaccgcgta aaagccattt
     8701 tcgtattgtt tagttaaatt aaaaaattct atttggctca tatcaccttt ttagtgtaat
     8761 tttatatact tctaacacat cttggccgtc ttgcgttttg aaaatagttc cctctactgt
     8821 agcgtataga ccttctgata cttcaagctt ttggtcttta gcataaagat agataatatc
     8881 gttgttatcc tcatccaata atttatattt aatcttttcg tcaggcattg acagctctcc
     8941 cgcaaagact atctttcctg tgtagcttat ggaaaccttt tgtggttcag gttggccgct
     9001 tactaaaagt tcatcattta ttaatttttt atcgataaag taattaagga gtcctcctgt
     9061 tgcaaaaaac agcaaaaaga ttccaaatag caatattagg agtttgggcc cttttagagc
     9121 ctcgattctt gagtatatgg ttttaagctg ggcaagcata ctttaatagt attataccct
     9181 aaaagcttaa tatattcata caactatgta tggaagatat tcagtaaaat atattaccag
     9241 aaataatgat aatccggatt cttgtactat ctaccttttt gatgaagctg agaaagtact
     9301 agtgccaat
//
LOCUS       DAOSWN010000023         8972 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_4968_length_8972_cov_4.119210, whole genome shotgun sequence.
ACCESSION   DAOSWN010000023 DAOSWN010000000
VERSION     DAOSWN010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8972
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4968_length_8972_cov_4.119210"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            262..657
                     /locus_tag="PLF29_01820"
     CDS             262..657
                     /locus_tag="PLF29_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52705.1"
                     /translation="MKKLIGSVATLLIPMAICAVGVFLVAQPPKTKVAQPTTTVAEQI
                     TPTTAPAVVEEKDEGLLVWAGNAFVLAFALGIAALLVIVALRRSGWSMEDIIPPSPGD
                     DGNGCCLFVVVVMGVLAAGGIYYLLNYFF"
     gene            1099..1509
                     /gene="mraZ"
                     /locus_tag="PLF29_01825"
     CDS             1099..1509
                     /gene="mraZ"
                     /locus_tag="PLF29_01825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HXK52706.1"
                     /translation="MKMFLGEYQPNITEGSRLALPKKLRDQILGDEVVLSRGFEQCVF
                     IYDKNDWLEESQKQLSGSITEARTRDLKRYMYAGAAEAAIDSQGRIVLPSNLKVYAQL
                     DKETTVIGAGDHIEIWNTSIWEAYLENLSKNLPS"
     gene            1516..2418
                     /gene="rsmH"
                     /locus_tag="PLF29_01830"
     CDS             1516..2418
                     /gene="rsmH"
                     /locus_tag="PLF29_01830"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HXK52707.1"
                     /translation="MYHTPVFESEILTYMAPIKGGKYIDCTLGDAGHASKLLEKGCFV
                     LGIDCNEFSLSRAEERLKEERLDKNFIGVKGNFKDILAIALENGFTDVDGIIYDLGFS
                     SSQVESGESGISFTNELDAPLDMRLDKNLGVTAADLLNTLSEQQLAQMFYVFGGERMA
                     KKFAVAIVRFRGSKKFNVIRDLVDLLVSEAPSGYEKGRIHPATRVFQALRIVVNDELA
                     NLENSLPQAACLLKLPRGRMAVISFHSLEDKVVKDFGRQYMRPASEIRLDSLTKKPLV
                     PSDGEVRNNRRARSAKLRVFERVA"
     gene            2420..2746
                     /locus_tag="PLF29_01835"
     CDS             2420..2746
                     /locus_tag="PLF29_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52708.1"
                     /translation="MDYRYKKTNKSTFDFCKAMLAVCVLIFTLSLIFQLSISNNLAVK
                     GKEMSALMREKTSLLSEISELELESAKYSSLSYIEKQAQEQGFQRNTAYVYALSPVVQ
                     TASLTQ"
     gene            2766..4667
                     /locus_tag="PLF29_01840"
     CDS             2766..4667
                     /locus_tag="PLF29_01840"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HXK52709.1"
                     /translation="MRRPISPTSNNSRIYILQLTASLGVLVLIVRLFFIQVLGHEKYK
                     ALAQDQYWNLQEIPAKRGDILSSDSYKLATTQVSFLLYAQPKVLLNINDAAQRLSETL
                     TSFVVFEDERSRLEYKEKTYAKIYDSLNLDLYWVRLASGLDPDQKAAIESLGITGIGF
                     EEQPARYYPEGSLASHVLGFVASNEEGNPQGYFGIEGALNGDLQGRPGRIVEERDALG
                     NPILVGGYKRIDSIDGRDISLSINRAVQYMVESRLKEGVETYGAISGSVIVMDPFTGD
                     VIAMANYPTYDPTDFSDFGMSEEENLEGGKKKIERTNLAISQTYEPGSVIKSLTVSAG
                     IDLGKIVPESTFVDAGPVVYSDYTINNWDGRHHGVQTIVQLLQKSNNIGAAWVGHLVG
                     ASSIVEYFRNFGLGNVTGIELEGEDTGLIKDADTLTNIDLATMSFGQGMSATPLQVLN
                     AFNSIVNGGNLMRPRIVQKIIESDRVIDIPAQVLRRVISKEASATMTGMLTQAVSGGE
                     SRYFNLKGYDIAGKTGTAQIPVNGKYDPSKTNATFIGYLPTSKKFTMLVKLQEPSSST
                     YASETAVPLWMQIANDLVNYYGIAPDDPSAVEPLKSPQLISEDPEAPKEESAVGVVED
                     VPEDSILEQ"
     gene            4740..5090
                     /locus_tag="PLF29_01845"
     CDS             4740..5090
                     /locus_tag="PLF29_01845"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HXK52710.1"
                     /translation="MHKTRKIGRGAESKVCDHLSSLGFSLLDKNWTCRWGELDVVAKK
                     RDLIAFVEVKYRRDCETDVERLINYRKRQAMSRAIRYYLYSNRIAYSDYKLKCYLFFV
                     SPTYLYCYTLPLLQ"
     gene            complement(5209..6291)
                     /locus_tag="PLF29_01850"
     CDS             complement(5209..6291)
                     /locus_tag="PLF29_01850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HXK52711.1"
                     /translation="MFKKALVSTTLLILLATGLYITYDFLALDNKINPENSIISPIIN
                     AVEEKVDSSKNIEGSKYENSDIINILLLGIDRRSKEEYGYRTDIMILLTINKAKNKIV
                     LTSIPRDLWYGGGRVNAFFIQSSWEETQNAFLEITGQKPDAYILTDFEDFSWIVDQIG
                     GLPVTIETTFTDSQYPVDATKEYQTITFIQGQEKMSGDRALIFARSRKGDNDNGDWGR
                     MKRQHLLLKSLGENMFNADSFICKIGNLKITDGSCFLNLNTETLKKGFELATTGRMET
                     NLTLKDLVYAKDLYVDKNDYKVESLYMDYNYLYTPLAEDYGGAWVLAPIGDSFVNFHQ
                     ELENKINNTEIQELDRASDEAQTLNN"
     gene            6354..6755
                     /locus_tag="PLF29_01855"
     CDS             6354..6755
                     /locus_tag="PLF29_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXK52712.1"
                     /translation="MAKNILIIEDDLDIYNLYKTQLELSGYSVMNVSAGDKAIDAVRE
                     HKPDLVLLDLVLPGKNGLEILQEIKEDSELRSQRVVMLTNFGNEDNISKAIELGAEDY
                     IMKYNIVPEELAAKVSTLLGDASDSVVKLMS"
     gene            6757..7530
                     /locus_tag="PLF29_01860"
     CDS             6757..7530
                     /locus_tag="PLF29_01860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXK52713.1"
                     /translation="MDILLILPPFLIALVSIFYIHFTNQKATRLKKLQNEFTAMMIHE
                     LRSPLSVIRSSADMILKEKDRLGEEKVSLMIKQIEDSSSNLLSIVNDLLDVAKIEASK
                     IELFKHPVNLNELLESVMEGYSLLAKEKGLTAVVELDPTISYVSCDPEQLKHVLSNLL
                     SNAVKYTAKGHICLRSKQSHGFVQIEVCDTGVGITPKMRQQIFKKFVQARELPVSREH
                     GTGLGLVIAKGIVEAHGGKIWVEDNLPSGSKFIFTIPLS"
     gene            7637..>8972
                     /locus_tag="PLF29_01865"
     CDS             7637..>8972
                     /locus_tag="PLF29_01865"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HXK52714.1"
                     /translation="MLANDNIFRTYDIRGIVDKDLDVKGAYLIGCAVGTLLFEKLAKE
                     NVTVVVGRDSRPSSGSLQSFFIEGLVAVGADVIDIGVSLTPIVHFLTCTQDFDAGIEI
                     TASHNPLSYNGFRLDYRNAVPFSSADIQEIRDKVRARNFEFNKGTKRKEDLNQQYFSY
                     IRDYFVFNKKHKVILNCGHSTISEYATETYGALNLDLTVINNEPEYGVDSVTPNPENK
                     FYMNSFKEVALSYDSSFELGIALDPDCDRMGLIDEKGNSYSTDRLVQAMLPYYLEGRS
                     GKSLVVYDPKCSSYLHERVLSLGGSPRMIKTGHTFFSDAMHSGAVLGCENSGHIYLDI
                     NYGYDDGIFNSLYFLKMLEDLDISLFELMKGVQKSYTSGEIKIPVSDEKKFSVISGIV
                     QNVKEQEANFYRVSYVDGVRVSLSEHSWFLIRVSNTTPNLTILIELDNIDLLN"
BASE COUNT         2565 a         1651 c         2002 g         2754 t
ORIGIN      
        1 gcttgcatct gccgatggtc tacgttaacg accagggaaa gaccttacac ccagggaccg
       61 gtgcggtgta acccgcatct ttggatgtcc cgacccccta tagggtgtaa ggagcgtggc
      121 gccagccacc aaattcccaa aagggaccta tccctgccaa tcttttcctc ggcaaaatag
      181 gcaggctaca ttaaagcctg gctccgacat taaggcttcg gccgaaatgt ctcctcggag
      241 cgaatttccc tggagggaaa aatgaaaaaa cttatagggt cggtagcgac cctacttata
      301 cccatggcca tttgtgccgt gggtgtgttt ttggtggcgc agccgccgaa aaccaaagtt
      361 gcccagccca ccactacggt ggctgagcaa attactccga ccacagcgcc cgctgtggtc
      421 gaggaaaagg acgagggtct cctcgtctgg gcaggcaatg cctttgtcct agcgtttgcg
      481 ctaggcatag ctgcattgct tgtcatagtt gcgctccggc gatctggctg gagcatggaa
      541 gatataatac cgccgtcacc cggcgacgat ggcaacggtt gttgcctgtt tgtcgttgtg
      601 gtaatggggg tcttggcagc aggtggtatt tactacctgc tgaactattt tttctaaggg
      661 ggggcggagg gaaggccttg cattacaggt tttcctgaat cccctccttt ttcattactt
      721 atagttccgt tacttgaaac tgcttgcgta agctagtaga tgtagtttac ccaacatgtt
      781 gtgcttcgca gcgcaaagaa attacagaaa aacacatgca taagccggtg cggttgacgt
      841 tcttcagctt ttagcttatc ggcgaaggct gaaaggagta aaagtgtaga tgcgccaaca
      901 ttctaaaagc atcagcagga aaaagttccg agaacagagt tttaaaattt cactttcaag
      961 tcatctcctt tcaatcttcg ggtaatcttt gtattgacct tgggataaaa ttggtgcata
     1021 ttggatgtac atggtgaatg ctggtgaata tctatagatt tcacaagcat tttgtctgct
     1081 cattaaagtc taatatttat gaaaatgttt ttaggggagt atcaaccgaa tattactgag
     1141 ggttcaaggc tcgccctgcc taaaaaacta cgtgaccaga ttttaggaga tgaggttgtt
     1201 ctctctagag gttttgagca gtgcgttttt atttacgata aaaatgactg gctcgaagaa
     1261 tctcaaaaac agctttcggg ttcaataacc gaggctagaa ccagagacct taaaagatat
     1321 atgtatgccg gtgccgctga agctgcaatt gattcgcaag gtcggattgt tttaccttct
     1381 aatctaaagg tctatgctca gcttgacaaa gaaacaacag taattggagc aggagatcac
     1441 attgaaatat ggaacacttc catctgggaa gcgtaccttg aaaatttatc gaagaacttg
     1501 ccgagttagg ttgaaatgta tcacacacct gtatttgaga gtgaaatttt aacttatatg
     1561 gcgcccataa aaggcggaaa gtacattgat tgtactcttg gagatgctgg gcatgcctct
     1621 aaattgcttg aaaaaggatg ttttgtcctt ggtattgatt gtaatgaatt ctctctaagt
     1681 agagccgaag agcgccttaa ggaggagagg ctggataaga attttattgg ggtaaaggga
     1741 aattttaaag atatactcgc tattgctctt gagaatggtt ttacagatgt tgatgggata
     1801 atttatgatc tcggttttag ttcttctcag gttgagtcgg gagagtcggg aattagtttt
     1861 acaaatgaac tagatgctcc gctggacatg aggctagata aaaatttggg tgtaacagcg
     1921 gctgatcttc tgaatactct ttcggaacag cagttggcgc agatgtttta cgtctttggg
     1981 ggtgagcgca tggcgaaaaa gttcgctgtt gccatagtta ggtttcgtgg atcgaagaag
     2041 tttaatgtaa ttagagatct tgttgatctt ttggttagtg aagctccttc gggctatgaa
     2101 aagggcagaa ttcatcctgc cactagagta tttcaagccc tgcgaatagt tgtcaatgat
     2161 gaattggcaa atcttgaaaa ttcactgcct caggccgcat gtttattaaa actgcctagg
     2221 gggcgaatgg ctgtaattag ttttcattcg ctcgaagata aggtcgtcaa agattttggt
     2281 cgacaataca tgcggcctgc tagtgaaata cggcttgata gtttaactaa aaagccttta
     2341 gtcccttccg acggggaagt gagaaacaac agacgcgcaa gaagcgccaa attaagagtt
     2401 tttgaaagag tagcttagta tggattatag atataaaaaa acaaacaaaa gcacattcga
     2461 tttctgcaaa gctatgcttg ctgtttgtgt tttgattttt acgctaagtt taatatttca
     2521 gcttagcata tccaataatt tagctgtaaa gggtaaagag atgtccgcac taatgagaga
     2581 aaaaacttct ttgttgtcgg aaatatcgga gctggaactt gaatccgcta agtattcgtc
     2641 tttgtcatac attgaaaaac aagcccagga gcaaggattt cagagaaata ccgcttatgt
     2701 gtatgccctt agccctgttg tacaaactgc atctttgact caatagtttg atattataga
     2761 gttacatgag aagaccgatt tcgcccacaa gcaacaatag cagaatctat attttacagt
     2821 tgaccgcttc tctaggcgtt ttagttctta ttgttaggct ttttttcata caagttttgg
     2881 ggcacgaaaa gtataaagct ttagctcagg atcagtattg gaatttgcaa gaaatacccg
     2941 ccaaaagggg agatatttta agtagtgact cctataaatt agccacaacc caagtttcat
     3001 ttttattgta tgctcagcca aaggtattgc ttaatataaa cgatgcagcc cagcgcctat
     3061 ccgaaacttt aacaagcttt gttgtctttg aagatgaacg cagtcgcctt gaatataaag
     3121 aaaaaactta tgcaaaaatc tatgactcct taaatttaga tttatattgg gtaaggttag
     3181 cgagcggctt ggatcctgat cagaaggccg caatagagag cttgggaatt acaggcattg
     3241 gttttgaaga acagcccgca aggtactacc cggaaggttc tttagcttct catgttttgg
     3301 gttttgtagc tagtaatgaa gaagggaacc ctcagggtta ctttggaata gaaggtgcgc
     3361 tgaacggaga tttacaaggt cgccccggta gaatagtaga ggaacgcgat gctcttggga
     3421 accctatttt ggttggaggt tataaacgaa tagactctat agatggcagg gatatatcgc
     3481 tttcaattaa tcgcgcggtg cagtacatgg tggaaagcag attgaaggag ggggttgaaa
     3541 catacggcgc aatttccggc agtgttattg ttatggaccc ttttaccgga gatgttattg
     3601 ctatggctaa ttatccaacg tacgatccga ctgatttcag tgatttcggt atgtccgaag
     3661 aagaaaatct tgaaggtggc aagaagaaga ttgaacgaac caatttagca atatctcaga
     3721 cctatgagcc gggatctgta attaaatctt tgacggtttc tgcgggcatt gatctgggaa
     3781 agatcgttcc agaatctact tttgttgatg caggccctgt tgtttattct gattacacta
     3841 taaataactg ggatggaagg catcacggtg ttcaaacaat agttcagctc ttgcaaaagt
     3901 caaataacat tggcgcggca tgggtgggcc atttggttgg agctagcagc atagtggagt
     3961 attttagaaa cttcggttta ggaaatgtaa ccggcattga gcttgaggga gaggacaccg
     4021 gtttaataaa ggatgcggat actttgacca acatcgattt agctacaatg tcgtttggcc
     4081 aaggcatgtc tgcgacgcct ctgcaggttc ttaatgcctt taattcaatt gttaacggag
     4141 gtaatctaat gcgcccccga attgttcaga aaattataga aagcgacaga gtaattgata
     4201 ttcccgccca agttttacgc agagttatat ccaaagaagc ttcagcgaca atgaccggca
     4261 tgttgactca ggccgtcagc ggcggtgaat ccaggtactt caatttaaag ggatacgata
     4321 ttgccggaaa gaccggtaca gcccaaattc cggttaatgg aaaatacgat ccttcaaaaa
     4381 ctaacgccac ttttatcggt tatttaccaa cctctaaaaa gtttacaatg cttgtgaaat
     4441 tacaagaacc cagctccagt acatatgcat ctgagacagc tgttcctttg tggatgcaaa
     4501 tagcaaacga ccttgttaat tactatggta tagcccccga tgatccttct gccgtagagc
     4561 cgttaaagtc cccccagttg atatctgaag accccgaggc tccaaaggaa gaatcggctg
     4621 tcggcgttgt tgaagatgta cctgaagatt ctattttaga gcaatagtta tgcccgctta
     4681 atctattttt actagcattc tcccgtcatt tattctcact cttttaataa aatttttata
     4741 tgcacaaaac aagaaaaata ggtcgtggcg cagagtctaa ggtgtgcgac catttaagtt
     4801 ctttgggctt ttccttactg gataagaact ggacttgcag gtggggagaa ttggatgtgg
     4861 ttgcgaagaa aagagacctt atagcttttg tggaggttaa gtatagacgg gattgcgaaa
     4921 ctgatgtaga aaggctcatt aattacagaa agaggcaggc catgtctcga gctataagat
     4981 actatttgta ttcaaatcgc attgcttatt ccgactataa actaaagtgt tacttgtttt
     5041 ttgtatcgcc aacatatctt tactgttata cactcccgct attgcaatag tgctatattg
     5101 cgagtagata agtttttcag acgaactttt tcgaatagag gttttagttt aggtgaattt
     5161 tgctcatttt ttagataggg tttgctatta acctttatgc ctagggcttt agttgttgag
     5221 tgtttgcgct tcgtcgctcg cccgatctag ttcttggatc tcagtattgt taattttatt
     5281 ttccagttcc tggtgaaaat taacaaaaga gtcacctatt ggagcaagta cccaagctcc
     5341 cccatagtct tcggctagag gagtgtataa atagttgtag tccatgtaca aagattccac
     5401 tttgtagtcg tttttatcta catacaggtc ttttgcgtac accaggtctt ttagtgttag
     5461 gttggtttcc atcctgcctg tagtagcgag ctcaaagccc tttttcaagg tttccgtatt
     5521 aaggtttaaa aagcaggacc cgtcggttat ttttagattt cctattttac atatgaaact
     5581 atccgcatta aacatgtttt cgcctagact tttaagcaag agatgctgtc ttttcattct
     5641 gccccagtca ccgttgtcgt tatctccttt tctagatctt gcaaaaataa gtgcacggtc
     5701 gccggacatt ttttcttgac cttggataaa agttattgtt tgatactctt ttgttgcatc
     5761 gaccggatac tgagagtctg taaaggtggt ctctatcgtt accggaaggc ctcctatttg
     5821 atccacgatc cacgagaaat cctcaaaatc agtcaaaatg tatgcatcgg gcttctggcc
     5881 tgtaatttct aaaaaagcgt tttgtgtttc ttcccagcta ctttgtataa aaaaggcgtt
     5941 gactctacct cctccatacc aaagatctct ggggatagag gttaggacta ttttattttt
     6001 agctttattg attgttagta agatcattat atcggttctg tagccgtatt cttctttact
     6061 tcgcctgtca attccaagca gaagtatgtt aataatgtcc gaattttcgt attttgaacc
     6121 ctcaatgttt tttgaagaat caactttttc ttcaaccgcg tttataatgg gagaaattat
     6181 gctgttttcg ggatttattt tgttgtctag tgctaaaaaa tcgtaggtta tataaagtcc
     6241 tgtggccagc aatattagaa gtgttgttga tactaaggct tttttaaaca tactaaataa
     6301 gcatagagta aatgttattg tttgtgaagt gtgtgctgtg ttatatttta catatggcta
     6361 aaaacatact tataatcgaa gacgacttag atatttataa cttatataaa acccaacttg
     6421 aactcagcgg ctactctgta atgaacgttt ctgcaggaga taaagccata gacgcggtaa
     6481 gagaacataa gccggacctg gtattgctcg accttgtttt accgggtaaa aacggccttg
     6541 aaatcctgca agaaataaaa gaagatagcg agctcagaag ccagcgcgtt gttatgctga
     6601 caaatttcgg taatgaagat aacattagca aagctattga gcttggtgca gaagattata
     6661 ttatgaaata taacattgtt cctgaggagc ttgctgccaa agtatccacc ttgctgggtg
     6721 atgcctctga ctcggtagtt aagttaatgt cttgaaatgg atattttgct aattctgccc
     6781 ccatttttaa ttgctttagt ttctatattt tatatacatt tcacaaatca aaaggccacg
     6841 aggcttaaaa agcttcaaaa cgaattcacc gcaatgatga tccacgaatt aaggtctcct
     6901 ttgtcggtca ttagaagttc tgccgacatg attcttaaag aaaaagatcg tttaggagag
     6961 gaaaaagttt ccttgatgat taaacagatc gaagattcct cgtctaacct tttatctata
     7021 gtgaatgact tgttggatgt tgctaaaata gaagctagta agattgagct ttttaagcac
     7081 ccggttaatt taaatgagct tttggaatct gtaatggaag gctacagcct tttggctaag
     7141 gaaaagggtc ttacagctgt tgttgagctc gatcctacga tttcttatgt aagctgcgac
     7201 cccgaacaat taaagcacgt tttgagtaat ttactatcca atgcagtaaa atacactgct
     7261 aaggggcaca tttgccttcg atcaaagcaa tctcatggtt ttgttcagat agaggtttgc
     7321 gatacaggag tcggaattac tcctaagatg cggcagcaga tattcaagaa attcgttcag
     7381 gccagagaat tgcctgtgtc tcgagaacac gggacaggtc taggcttggt tattgcaaaa
     7441 ggaattgtcg aggcacacgg cggtaagata tgggttgagg acaatctacc ctcaggatca
     7501 aaatttattt tcacaatccc gctttcttaa ttcggtgtcc ttttaaagct ctgcactatt
     7561 tctttattct ttacgttcgt attgccgcat cgtgtacttt aaacatttaa tattccctga
     7621 taatattacg tatagcatgt tagcaaatga caatattttt cgtacatacg atataagggg
     7681 cattgtagat aaagatcttg atgtaaaggg cgcttactta atagggtgtg cggttggaac
     7741 tttactgttt gaaaaactag caaaagaaaa tgttacagtt gtcgttggaa gagattctcg
     7801 acccagctcg ggttctttgc aaagtttttt tattgaaggt ttggttgcgg ttggcgctga
     7861 tgttatagat attggagtct cattaacccc aatagtgcat tttttaactt gtactcaaga
     7921 ttttgatgct ggaatagaaa taacagcaag ccataacccc ttatcgtaca atgggtttcg
     7981 ccttgactac agaaatgcag ttccttttag ttctgctgac atccaggaga ttagagacaa
     8041 ggtacgtgca cgaaattttg aatttaataa agggacaaag agaaaagaag acttaaatca
     8101 gcaatatttt tcttacataa gagattattt tgtatttaac aaaaaacata aagttatatt
     8161 aaattgcgga cactcaacca tttcagaata tgcgactgaa acttatggtg cgcttaacct
     8221 tgacttgact gttataaaca acgagcctga gtacggtgtc gattcagtga ccccgaatcc
     8281 tgaaaacaag ttttatatga actcctttaa agaagttgca ctttcttatg attcttcttt
     8341 tgagctcgga attgctttag atcctgattg cgatcgtatg ggcttaattg acgaaaaggg
     8401 caatagctac tctacagaca gacttgtcca agcaatgctg ccttactact tggagggccg
     8461 ctccggaaaa tctttagtgg tttatgatcc taagtgctcc agttatttgc acgaacgcgt
     8521 tcttagtctt gggggatctc cgcgcatgat aaaaaccggt cacacatttt tttcggatgc
     8581 gatgcactcc ggtgcagttt taggctgcga aaactcaggg catatatatt tagacataaa
     8641 ttatgggtat gacgatggta tttttaattc cttatatttt ttaaagatgc tggaagatct
     8701 ggacatcagc ttatttgagt taatgaaggg cgtgcaaaaa agttacactt cgggagaaat
     8761 aaaaatacct gtaagtgacg aaaaaaagtt ctcggtaatt tccggtatag tccaaaatgt
     8821 aaaggagcaa gaggcaaatt tttatcgagt aagctatgtt gatggtgtta gagttagttt
     8881 aagtgaacat tcttggtttt taattcgcgt atccaatacg actccaaact taacaatcct
     8941 tatcgagcta gataatatcg atcttttaaa tg
//
LOCUS       DAOSWN010000024         8836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_5072_length_8836_cov_4.858330, whole genome shotgun sequence.
ACCESSION   DAOSWN010000024 DAOSWN010000000
VERSION     DAOSWN010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8836
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5072_length_8836_cov_4.858330"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PLF29_01870"
     CDS             <1..630
                     /locus_tag="PLF29_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HXK52715.1"
                     /translation="VLGTSQPPGFPFALNSAVDALLKKEFDYHRTSDKEHPLITKYGV
                     DAKPVAHESLDDWRNNFVGVQYLHEPTNLLVFGAIDDLWISSKGEYIVVDYKSTSKSE
                     EITELNKDWQDGYKRQMEVYQWLLRNNGYKVSDTGYFVYCNGNTDLEAFDAKLEFDVT
                     LISYTGDDSWVEQTIFDIKKCLDSDKIPESAPDCDYCAYYNTRDKHEKI"
     gene            1019..1918
                     /locus_tag="PLF29_01875"
     CDS             1019..1918
                     /locus_tag="PLF29_01875"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013622468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HXK52716.1"
                     /translation="MSIPTFDETFLPILNILSKGETLKTSDLPSLLLEDGHFSLSEEE
                     QKKKISSGSNMFSNRVYWGVTYLRQAKLVERPSRGFVKITQKGSDYLATNPSELTLSW
                     IKQDSDYQAYEPVRSKQKDEEESFDIGGMSPQDLIDQGVRKLNEQLKNDLIEKLHESN
                     PYYFEKIVLILFKKMGYGDFQETPKSGDGGIDGIINNDLLGLEKIYIQAKRYAETNKV
                     REPEIRNFIGAMSGDVSRGIFVTTSEFDDSAKQKARDAHNHKIILIDGDRLTDLMIEY
                     NVGVQVQASFDVKDVDEDFFELG"
     gene            complement(3401..3475)
                     /locus_tag="PLF29_01880"
     tRNA            complement(3401..3475)
                     /locus_tag="PLF29_01880"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3441..3443),aa:Lys,seq:ttt)
     gene            complement(3543..3923)
                     /gene="rplS"
                     /locus_tag="PLF29_01885"
     CDS             complement(3543..3923)
                     /gene="rplS"
                     /locus_tag="PLF29_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HXK52717.1"
                     /translation="MSQEQITQSNSAVIATPADIPNFRVGDTVKVHYKITEGNKTRTQ
                     PYEGIVIAKKGEGMSKTFMVRKIGADNIGVERIFPLYSPNIEKLEVTRMGKVRRAKLY
                     YLRDKKGKAAVRVKERQPKKIDTE"
     gene            complement(4039..6135)
                     /locus_tag="PLF29_01890"
     CDS             complement(4039..6135)
                     /locus_tag="PLF29_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HXK52718.1"
                     /translation="MAVICHRCGQRPAVISANRIIDGKQVYMALCEVCYNEIQKEATS
                     SSSRLDKFGRDLTEAAKEGKLDPVIGRKDEIERVVHILSRRTKNNPVLIGDPGVGKTA
                     IAEGLAQRIVKGQVPEPLRNKRVFALDLALVIAGTSHRGMFEKRLKDIIEEVISSHGQ
                     IILFLDELHTVVGAGAAEGSMDAANILKPALARGELQMVGATTIDEYRKYIEKDAALE
                     RRFQSILVDEPTSEETVEIIKGLRERYEAHHQVKITDEAISAAVKMSDRYISDRFLPD
                     KAIDLIDEASALVRLSQVKEPDNLKQVEEQLYTKKADLRKKDIAPEQKAELEEDIKKL
                     EQVKEELIDIWTKTKLEEIPNVLADDVLKVVSRATRIPLERLSVHEKEKLVHLEELLH
                     KRIVGQDVAVKTVSEAIRRARAGLKDPRRPIGSFLFLGPTGIGKTELSKALAEVLYGN
                     EDMIVRLDMSEYMEKHTVSKLIGAPPGYIGFERGGNLTDIVRRKPFSVVLLDEIEKAH
                     PDIFNTLLQIMEDGRLTDSKGRTVDFKNTIVIMTSNVGSEYLRRGSIGFGSKEAKQNI
                     TGKSEFEQNVDKVLKDSFKPEFLNRIDEIIIFTSLNKDDIKEICIRLLEKTVMALKDQ
                     NITLKWSKKLVDYLVENGYSDEYGARPLRRLIQKEIENKVADKIILGEINEGDTVSLD
                     AVSEGIRVSVKSPALV"
     gene            complement(6351..8195)
                     /locus_tag="PLF29_01895"
     CDS             complement(6351..8195)
                     /locus_tag="PLF29_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HXK52719.1"
                     /translation="MDLKFDPENPKYEYIKTMSQAEKALEKLESAQIIAIDVEANSLD
                     PYTGILLLVQIGTDEISYIFDARTLDLANYPRFASLLQDKSKVKLFHNSKFDYKYIKQ
                     HLGIECNNIYDTMLAERILLAGRKQKVGLGALCDRYLSPGILDKQLQSSFIDMTKHTP
                     VTEAQLRYAGADTLVLFPLFERQIQKLKQENLLNIAKLEFAVAPVVGNMELNGMFIDK
                     KKWMGIIRNLEIKRDEVAKSFQEAVRPYYGTTQLDLFGGVADAININSQQQLMDLFNN
                     KLKLDMPSTGTQILNDVSHPVVDLLKKYRGYEKLISAFGESLIAKINPKTHRIHPDFL
                     QLQTETGRFACSNPNIQQIPRNTPEAPFRECVNPEPGYKLVISDYSGFEMRILADLSG
                     DENMLKVFTDKLDIHSYTASLMFDKPYSDDFKHKYPDLRQMAKPIGFGLMYGMGAVGL
                     VSRFKLIGEEVTVDQSQDLIDKYFKSYPGVRKFLETVAKKAVIDGYSMTPGGRKRWYE
                     VPSKTDPEYRRKLGKIQREAKNHPIQGTNADAIKYALVFCQERMRKEGIDGKIILTVH
                     DEIGCEVREDQAEDWGRILSEEMVRAGELFLKKVPIRSDPFVGDVWEH"
     gene            complement(8216..>8836)
                     /locus_tag="PLF29_01900"
     CDS             complement(8216..>8836)
                     /locus_tag="PLF29_01900"
                     /inference="COORDINATES: protein motif:HMM:NF014674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52720.1"
                     /translation="EVKRQTEILIGGKMPAQENRGYDEKADATLPQRSKEDAHDYRYF
                     PDPDIPPMRFDQAYFDGLKSSMPELPGAAYLRLTSVLGLSAVAAEMLTSGPGLGLLQK
                     FNDLSQVADPVKVANLLLNKPGYRDLEKDEFLQKMQSDESTIDDADIILATVKNVIAQ
                     NNKAVEDYRSGKDSAIQYLVGQVMRETKGKANPGLSEELILKELMS"
BASE COUNT         2658 a         1802 c         1591 g         2785 t
ORIGIN      
        1 gttttaggaa cttcccaacc cccaggattt ccttttgcgc tgaacagtgc agttgacgcg
       61 ttacttaaaa aagaatttga ttatcatcgt acatctgata aagaacaccc acttattaca
      121 aaatatggtg tagatgcgaa accagtagca catgaaagtc ttgatgactg gcgaaataac
      181 tttgttgggg ttcagtatct tcatgaacca acaaatctac ttgtttttgg cgcgattgat
      241 gatttatgga tcagctcaaa aggagaatac attgttgtag attacaaatc caccagtaag
      301 agcgaagaaa taactgaact taacaaagac tggcaagatg gctacaagcg ccaaatggaa
      361 gtataccagt ggcttttaag aaacaacggg tacaaggtat ctgatacagg ttactttgtt
      421 tactgcaatg gaaacaccga tctagaagct tttgacgcca aactagagtt tgatgtaact
      481 cttatttctt atactggtga cgactcttgg gtggaacaga caatctttga tattaagaag
      541 tgtttggatt cagataagat acctgaaagc gcacctgatt gtgattactg tgcgtattac
      601 aacactagag ataaacacga gaagatataa aacacagttt tcaaaaaacc tgacctgtta
      661 aaactgtcta cggtacatca aatccaaagc aaaaaataga tttttagacc tgcggtggtt
      721 ttcaagattg ttgtgcttta aagcatagac ctaccatgtt atgcaacatt tctcattttt
      781 atatatttgt tacaacacac ttttatactt ataaatgtta cacaacattt ttgttgtaac
      841 tgttgtaaca acgaatacta aagttacagc agattgaaaa tgtgtagaca aatatgtgta
      901 agtttataag catttgtgtt gtaacattgt gatacaagcc aaactgttaa cacatttatt
      961 attacaataa ctaagctata gatgttgagg tataatctat tcataaggat taaaaaatat
     1021 gtctatacca acatttgatg aaacattctt accaatattg aacattctct caaaaggaga
     1081 aactttaaag acatctgatc ttcccagtct tttattggaa gatgggcatt tctcgttatc
     1141 cgaagaagaa cagaagaaaa agatatctag tggttcaaat atgttttcca atagagtcta
     1201 ctggggagtt acatatctca gacaagccaa acttgtagag cgcccctcta ggggatttgt
     1261 aaaaataact caaaaaggtt ctgactatct agcaaccaat ccaagtgagt taacacttag
     1321 ctggattaaa caagattcgg attatcaggc atatgaacca gtgcgatcaa agcaaaaaga
     1381 tgaagaagaa agttttgata ttggaggcat gtctcctcaa gacctgatag atcaaggtgt
     1441 gaggaaactc aacgagcaat taaagaatga cttaatagaa aaacttcacg aaagcaatcc
     1501 ttactacttt gaaaagattg ttcttatact ctttaaaaag atgggttatg gtgatttcca
     1561 agaaactcca aagtctggtg atggcggaat agatggaata ataaataacg atctactagg
     1621 tcttgaaaaa atctatatac aagcaaaaag atatgcggaa acaaataagg ttagagaacc
     1681 agaaatacga aatttcattg gcgcaatgag tggcgatgtg agcaggggta tatttgttac
     1741 aacatctgaa tttgatgact ccgcaaaaca aaaggcaaga gacgctcaca accacaaaat
     1801 aatacttata gatggtgata gattaactga tttgatgatc gaatataatg taggcgttca
     1861 agtacaagcc tcttttgacg ttaaagatgt tgatgaagac ttctttgaat taggttaata
     1921 caactctcgc attgcgagag tcctgtatgg ttgcacactt cctaaaatcc tttaccatgg
     1981 agctgtagct atcctatgaa aaacacagct atcgcttaca ttcgcgtaag ctcagaagaa
     2041 caagtaaaaa actactcact agctaatcaa aaagaatatt gtgaaaaata tgcgcgggaa
     2101 aatgatttag atttagtaaa gatattcaaa gaagagggta aatccgcaat gaatattgat
     2161 cgtcccgctc taatttccct actagagtac tgtagaaaaa acactggcaa agtagattac
     2221 ttagttcttt ataaaataga cagactatca cgctcaacaa tcgattatct agaaataaaa
     2281 agacgtttag ctaaatacgg tgtacaagtt aaatctgtta cagagttcac agacgatact
     2341 cctatgggtg agttcatgga aactcttatt gccgcacaag ccaaactaga caatgctata
     2401 agagccgagc gggtaagtga tggtattaga aaaagagttg aatcaggact acctgttagc
     2461 gttccaccaa taggttataa gctatctaaa ggtaataacg gcaaaagcat tacaatacca
     2521 gacgaaccca ctttctccct tttacaaaga gctggttatc agtaccttac tggaaactat
     2581 actttcaccg aattggcgga gttactcaat aagtgggggt ttactacaaa gaaaggtaat
     2641 cccgcgacat ctaactttgt aggtaagttt ctaagaatgt ctttttacaa gggaattatc
     2701 tactcaaaga aatggaagaa agaatacaga ggtatgcata taccaatgtt tactgataac
     2761 gaatgggacg atatccaaga tattgtagac ggaaaccctt taacaattaa gaaaaagcaa
     2821 agagcagaag agtttcccct aagaggattc gttacgtgta gcgtaacagg agatccacta
     2881 acaggttctt ggtgtaaagg taatacaaaa cactacgcat actactattc accatcaaag
     2941 tataaatcta ttccaaaaga tactctagaa agtgaattta cggctttgtt ggaccttata
     3001 gagccagcgc caggtgttat agagtctttc tcacgtgttt tatttgataa atataatgaa
     3061 aagtactcaa agctactaca aaacgaacaa ctattatcag atcagataaa agatataaac
     3121 ggtgtgggaa atgaggtgga agatgacttg gcggtgaaaa acctaataaa aaatgaaaac
     3181 aaaataaata tgataactat agagaccgct aggaactttt cagaacattt cttagaacac
     3241 atatcccagt attggagaac tgcccaccta agagacaaag ttaaaataca agaagtatta
     3301 ttccctcagg gacttactta cacgtatcct gaatttttag aacctaggat aagctgtctt
     3361 tataagataa atacggaaaa gtcgactctc aatcatttat tggtgagggc ggtgaggatc
     3421 gaactcacga caccctggtt aaaagccagg tgctctacca ctgagctacg ccccctcgat
     3481 aagcttataa attgttacaa aaatatggaa gaagtgcaat atttttaaaa aatagctcgt
     3541 atttattcag tgtctatctt tttgggttgt cgttccttaa cccttacagc cgctttacct
     3601 tttttatctc gtaaataata aagcttggca cgtcgtactt tgcccattct tgtaacttcg
     3661 agtttttcaa tgtttggaga atataaaggg aaaattcttt ctacaccaat gttatcagca
     3721 ccgatttttc ttaccataaa ggtctttgac atgccctcgc cttttttggc aatcacaatg
     3781 ccttcatatg gctgggttct ggttttatta ccctctgtaa ttttataatg tacctttaca
     3841 gtatccccta ctctaaagtt tgggatgtcg gcaggagttg ctataacagc gctattagac
     3901 tgagttattt gttcttgtga catataccga gtgagtttaa caaattatta acctaaattc
     3961 aatacttact ttaacaattt atacaataga acaagcttag gggactttat tcaatgcgag
     4021 aagcgagccc gagttacctt aaaccagagc gggagactta actgatactc ttattccttc
     4081 ggaaacagcg tcgagagata ccgtgtcccc ttcatttatc tcgcctaaaa ttattttatc
     4141 tgctacctta ttctctattt ctttctgtat caaccttctc agcggccttg caccatattc
     4201 atcgctatat ccattttcta ccaaataatc aacgagtttc ttagaccact taagggtgat
     4261 gttttgatct tttagagcca ttacggtttt ctcaagaagt cttatacata tttccttgat
     4321 gtcgtcttta tttaaggatg taaatattat tatttcgtct attctgttca aaaactcagg
     4381 cttaaagctg tctttcagga ccttgtctac gttttgttca aattcggatt ttcctgttat
     4441 attttgtttt gcttccttag atccgaatcc tatcgagccc cgacgcaaat actcagaacc
     4501 tacattggag gtcattatca cgattgtatt tttgaagtct actgttcttc ctttactatc
     4561 agtcagcctt ccgtcctcca ttatctgaag cagggtgtta aaaatgtcgg gatgggcctt
     4621 ttcaatctca tctaatagaa ctactgagaa aggttttctt ctaactatgt cggtcaaatt
     4681 acctcctctt tcaaagccta tatatccggg aggagctcca ataagcttac tgactgtgtg
     4741 tttttccatg tactcgctca tgtctaaacg tactatcatg tcttcatttc cataaagcac
     4801 ctcagccaga gcttttgaga gttctgtctt accaatgccc gtaggaccta aaaataaaaa
     4861 tgaaccaata ggacgtcttg ggtctttaag gcccgccctg gctcggcgaa tagcttcaga
     4921 cactgttttt actgctacat cctgaccgac tatcctcttg tgtaataatt cttccaaatg
     4981 aaccagcttc tccttttcat gcacactaag acgttcaagc ggaattcttg tggcccttga
     5041 gacaaccttg agaacgtcgt ctgctaatac atttgggatt tcttcaagtt tagtctttgt
     5101 ccaaatatca ataagctctt ctttaacctg ctcaagtttc ttaatatctt cttctaattc
     5161 tgctttctgc tcaggagcaa tatctttttt tcgaagatcg gcttttttag tgtagagctg
     5221 ttcttcaacc tgcttaagat tgtccggctc tttaacctgt gaaagtctaa ccaaagctga
     5281 ggcttcatca attaagtcaa ttgctttatc tggtaaaaat ctgtcactta tatacctatc
     5341 agacatcttc actgctgcgg atatagcctc atccgtgatt tttacttgat gatgagcttc
     5401 atatctctct ctaagacctt ttatgatctc gacggtctcc tctgaggtcg gttcatcaac
     5461 taaaattgat tgaaaacgcc tctccagcgc tgcatctttt tcaatatatt ttctgtattc
     5521 atctatggtt gttgctccaa ccatctgaag ctctcccctt gcaagggccg gctttaaaat
     5581 attagcagca tccatagagc cttcggccgc tccggctcct actacagtgt gtagctcatc
     5641 taaaaataat atgatttgtc cgtgggaact tataacttct tcaataatgt cttttagtct
     5701 tttttcaaac attcctctgt gagatgttcc tgctattaca agagcgagat caagagcaaa
     5761 aacccttttg tttctgagag gttccggaac ctgtcctttg actattcttt gagctaaccc
     5821 ctccgcaatt gcagtcttcc ctaccccggg atctcctatt agaactgggt tattttttgt
     5881 tcttcgtgaa agaatgtgaa caactctctc tatttcatct tttcttccaa ttacggggtc
     5941 tagcttacct tcttttgcgg cctccgtaag atcgcgccca aacttatcca accttgaaga
     6001 gctggaagta gcctcttttt ggatttcgtt gtaacacacc tcacatagag ccatgtaaac
     6061 ttgcttaccg tcaattattc tattagcgga tattactgca ggtctctgcc cgcatcggtg
     6121 acatataaca gccataagtt tatattagca caacttagca cttgcgcaag tggtttgata
     6181 ataatttaat caaccgccgc cggcttatgc aggtggtttg ctgtaatttc tttttgctgc
     6241 aaagcccaac atgtttgtaa accaatctac tagcttacgc atatagtttt tagtaacgaa
     6301 attataaatt actcaagaaa ttctcttata gcacggcaga gggagaagac ttagtgctcc
     6361 cacacatcgc ctacaaaagg gtccgatcta attggaactt tctttaaaaa cagctctcct
     6421 gcacgaacca tttcttcaga taaaattctt ccccaatcct cagcctgatc ctccctaact
     6481 tcgcaaccaa tttcgtcgtg cacagtcaaa attattttac cgtcaatgcc ctcttttctc
     6541 atacgttcct ggcaaaaaac aagcgcgtac tttatggcgt cagcgtttgt accctgaatt
     6601 gggtgatttt ttgcctcgcg ttgaatttta ccaagctttc tacgatattc cggatcggtt
     6661 ttagaaggca cttcgtacca gcgttttctt cctccgggag tcatgctgta accatctata
     6721 acggcctttt tagcaacagt ctccaaaaac tttctcactc cgggatagct cttaaaatat
     6781 ttgtctatta ggtcttggct ttgatcgact gtgacttctt ctcctataag tttaaatctc
     6841 gatactaagc ctacggctcc catgccatac attaaaccaa aaccaatagg cttcgccatc
     6901 tgacgtaaat caggatattt gtgtttgaaa tcatcggagt atggcttatc gaacattaaa
     6961 gatgcagtat agctgtgtat atcgagctta tcggtaaata ctttcaacat attttcatca
     7021 cctgataaat cagccaaaat acgcatctca aaacccgaat agtcggatat tacaagcttg
     7081 taacccggct cgggattaac acattctcta aaaggtgctt cgggcgtatt tcgcggtatc
     7141 tgttgaatat taggattaga gcaagcaaaa cgacccgttt cggtttgaag ctgtaaaaaa
     7201 tccggatgaa ttctgtgggt ctttgggttt atttttgcaa ttaaggattc accgaaagct
     7261 gagatcaatt tttcgtaacc tctgtatttt ttaagtaaat ctacaaccgg atgactaaca
     7321 tcgtttaaaa tctgtgttcc tgtagagggc atatccagct taagcttgtt attaaaaaga
     7381 tccattaact gctgttggct atttatgttt attgcgtcag caacgccacc aaaaagatcc
     7441 aactgggttg ttccgtaata tgggcggact gcttcctgaa aggactttgc tacttcgtct
     7501 ctttttattt ccaagtttct aattattccc atccacttct ttttatcaat aaacattccg
     7561 ttaagctcca tgtttcctac aaccggggca acagcaaatt ctagctttgc gatatttaat
     7621 aagttttcct gtttcagttt ttgtatctgt ctttcaaaca aagggaatag aactaaagta
     7681 tcagcgcccg catatcgtaa ctgcgcctct gttacaggag tgtgtttagt catatcaata
     7741 aatgaacttt gaagttgctt gtccaatatt cccggagata ggtatctgtc acacaaagct
     7801 cccaaaccca ccttctgctt tcggcccgct aaaagtatac gctctgccaa cattgtgtcg
     7861 taaatattat tacactcaat tccaagatgt tgttttatat acttgtagtc aaactttgag
     7921 ttgtggaaaa gcttaacttt agatttatct tgcagtaaag aggcaaatct ggggtaattc
     7981 gctaaatcta gagttcgcgc gtcaaatatg taacttattt catcggtacc tatttgcact
     8041 agtaacaaaa tgccggtata ggggtctaaa gaatttgctt ctacgtctat tgcaatgatt
     8101 tgcgcacttt ccaacttctc caaagctttt tccgcttgag acatggtctt tatatattca
     8161 tatttgggat tttcgggatc aaattttaaa tccataaact ttcctcattg tacacctatg
     8221 acattaattc ttttaaaata agctcctctg ataatccggg gtttgctttg ccttttgttt
     8281 cacgcataac ctgccctact aaatactgaa ttgctgaatc ttttcccgac ctgtaatctt
     8341 cgactgcctt gttattttgt gcaataacgt tttttaccgt agcaagtatg atatcggcgt
     8401 catcgattgt tgattcgtct gattgcattt tttgcaaaaa ttcatctttt tcgagatcgc
     8461 gatatccggg cttatttaat aacaaattgg ctactttgac cggatctgct acttgggaaa
     8521 ggtcgttgaa tttttgcaac aaccccaaac cagggcctga agttaacatc tcagctgcaa
     8581 ctgcagacag gcctaaaact gacgtcaatc ttaaataggc agctccgggc aactcaggca
     8641 tggaggattt tagcccatca aaataagcct gatcaaacct catcggagga atgtccggat
     8701 cgggaaaata cctatagtca tgagcatctt ctttgcttct ctgcggtaaa gtggcgtcag
     8761 ctttttcatc ataccccctg ttttcttgtg ccggcatttt cccgccgatt agaatctcgg
     8821 tttgcctttt aacctc
//
LOCUS       DAOSWN010000025         8778 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_5112_length_8778_cov_4.165425, whole genome shotgun sequence.
ACCESSION   DAOSWN010000025 DAOSWN010000000
VERSION     DAOSWN010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8778
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5112_length_8778_cov_4.165425"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PLF29_01905"
     CDS             complement(<1..421)
                     /locus_tag="PLF29_01905"
                     /inference="COORDINATES: protein motif:HMM:NF014458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HXK52721.1"
                     /translation="MLDIKIIRENPEVVKKAIEDKNMNGVVDIDRLLQVDVEYRNLLK
                     KVEELRAIRNELSQDISKVSGDAREKLITQASDTKEELKGLEDKLLVLKNELDAMVMA
                     VPNIPSADVPVGRDESGNEILKHVGDIPKLDFEPKDHV"
     gene            complement(484..606)
                     /locus_tag="PLF29_01910"
     CDS             complement(484..606)
                     /locus_tag="PLF29_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52722.1"
                     /translation="MNFPITELLGGNAKSGMPVIAVNKYEEGWHLQYISIEPLG"
     gene            complement(836..2953)
                     /locus_tag="PLF29_01915"
     CDS             complement(836..2953)
                     /locus_tag="PLF29_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HXK52723.1"
                     /translation="MATLKELRDVRLQKLKNLQDLGVDPYPATSFRTHLISEVISSFG
                     KLGGKEVILAGWIMSLRRHGKLTFFDIKDFSGTIQVYIKEQNLEKVEHKNSEISYSEL
                     DLLDIGDYIECKGALVITNSGEQSIEPHSIRILAKSLRPLPDKHDGLKDRETRMRKRY
                     LDTRINDDVRDRYIRRSKFWAAHREFFEKRGFYEINIPVLEAVPGGADANPFVTHMDS
                     IDETLYLRISHELFLKRLIGGGYEKVYEIGPRFRNEGLSDEHLPEHMAMEVYWAYADW
                     QSGMDFIQDLFNYVIESLYGDKKKFSIRGFEVDFSKKEWERIDFSDIMKQRYGVDVHN
                     SSIEDLKLACEKAGIKAETENRPRLVDALWKEIRRTIGGPAFLINHPKYLSPLAKANP
                     KDTRITERFQPIIAGSELGNGWSEINSGLDQFERFSEQQSMRDSGDSEAQWLDIDYVE
                     MLEYGMPPTFGYGHSERVFWFLEDVTAREGVPFPQLKHVYDDANAEIYGLSKSDPLVE
                     DSLSKTDLNESLYISSEGFLPSRKEAEALLRDHVKDSYQIQHALMIARGLEAYAEKFN
                     EDRELWYITGLLHDLDYFEYPDEHPAKSLEWFKERNYPESLIHAVAAHAHMRTNVKPQ
                     TRLASALIAVDEMAGFLYAYALMRPEGYDGMKVSSVKKKFKDRAFAAKIDREEILYGV
                     EQLGEGFDAHVEFLINVYKNQAK"
     gene            3165..4130
                     /gene="pgk"
                     /locus_tag="PLF29_01920"
     CDS             3165..4130
                     /gene="pgk"
                     /locus_tag="PLF29_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HXK52724.1"
                     /translation="MKKITEATIAQGTRVFVSTDIDVPIDNGHIRNTFRLDRGLETLK
                     YIVDKGAIPVIGGHIKNPQGKYDPQLSTAPLKKYYNEKLGEGSYELLENLRFDIREDQ
                     NEDTYAQELANKTEVFVNENFATSHRTNASLVAITKYLPSFAGLNLSREIEALDKILI
                     DYKKPFTAVIGGAKVESKAPVIDSLCNIADNILLGGKIGLNYSTDNARVSSPADYADG
                     NQDIGPKTILNYTGYILSAQTLLWAGPMGAYEKDEFCIGTNLIADAIVEATKGGAYSI
                     IGGGDTITAYSKNHALGEVSFVSTGGGAMLTYLAHKNMPGIEALG"
     gene            4134..4436
                     /locus_tag="PLF29_01925"
     CDS             4134..4436
                     /locus_tag="PLF29_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52725.1"
                     /translation="MAKSLKVQFPERCIGCELCIMEIQRQLKKHGLEGSLIRVFKSAK
                     EGKEICETLEYSIDLDPSINSYDIEQIRKICPKSVFVVADMTEEENYEDALLEQKD"
     gene            4439..5272
                     /locus_tag="PLF29_01930"
     CDS             4439..5272
                     /locus_tag="PLF29_01930"
                     /inference="COORDINATES: protein motif:HMM:NF014750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HXK52726.1"
                     /translation="MHKSVLFVNLKNKTYEVKKFDELAKYIGGVAVGFKLLLDNIEHD
                     PIIFSIGPLNGVFPFASKTSIVLHNNGVVEDLYIGGSLSSRMAFSGVDSIVFIESAEE
                     PTYVEITNEEVFFKPITTDIDSLGLPGKRSVLKPIKNKYFLDDYFLTPELFFEEKLAK
                     KNVGGIVLTGTKTREIKNPEKYKELLHEILGKKSLMEIDEGDSPSCTFCPMGCSKSRT
                     GEIGGNILVHSMVGCKLSQKIYSDVGTIFSCLSVLGYSYTHEDIEDLPLLVKEVLEKL
                     N"
     gene            5272..5928
                     /locus_tag="PLF29_01935"
     CDS             5272..5928
                     /locus_tag="PLF29_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HXK52727.1"
                     /translation="MKYLVANWKSNMTVSEVIVWLKAWDKLIKSAESNDAVRVLVAPC
                     FIHLPLFTGLENIYSCGQDVSENSVGPYTGYVNATQLREFADYSIVGHSERNEDSETV
                     VKKINQCRNNGIEAIACFTHTENATEYSKFTKILAWEDPENISKQGKYAELRYGEINH
                     SIEKIYSNIEPDTVLLYGGSVNSNNISELTRIKSISGFLVGNASLDVAHFYKLYCSMI
                     "
     gene            6008..7072
                     /locus_tag="PLF29_01940"
     CDS             6008..7072
                     /locus_tag="PLF29_01940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HXK52728.1"
                     /translation="MKYIDAHKKINTITKPVAKKNSKGSFKGIATLALILLIFVAVGG
                     TVLLFKSKLNPVSIVASATKANIKETDGRTNILVLGSDKRDNGNAVTSVLTDTILLVS
                     IGRVEHDVLLLSMPRDLYVENSKGYSTKINAVYTLEGPEELQSVVEDVLGVPIHYYAV
                     VDFNLFVNAINVLGGIDVNVQNAFTDYQYPIEGKENAPIEERYETISFAAGKQHMDGE
                     TALKFARSRKGDNNEGTDFARAARQQLVIEAIKEKALSLKTVTNASKIKELYDLYAEG
                     IDTNLGFADVQGFYLLSQQINFDSIRSIVLDDRSEADKGGLLYAPIDTALYGNQYVLI
                     PQSGDYSQIHAYVQRYLFGE"
     gene            7075..7713
                     /locus_tag="PLF29_01945"
     CDS             7075..7713
                     /locus_tag="PLF29_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52729.1"
                     /translation="MIVLVHGNNSVNKADVVFKLSEKISTPRKYLSIDENSPTDVLTA
                     TATNNMFTGAPIVILDLGSNTKSDAKSFLDIFPRVPAKSLLILLAQKELPKSNQFLQH
                     AQQQGYKIILSNTTPTANIFSFVSSLYSKNRKKTYREYETLIAEGADPFYILSMVMFG
                     ARKQAKAVNGDKIDEKTLSLYKYIFETDLKTKTGKIPVDVALPLIIEKVLTS"
     gene            7717..8472
                     /locus_tag="PLF29_01950"
     CDS             7717..8472
                     /locus_tag="PLF29_01950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HXK52730.1"
                     /translation="MGKFKKKVYHKKYTNDQIYNNVNLGFTLKSSAVPLVLTVTACAI
                     FATQVLYPLINFTTQDLTSVAAQHTALAKISGFREFQYSELQRNVLGSTNNSEQVQEK
                     FYISIPKLNIENAEVETNSSNLNPETALGHYLGSALPGQNGTAFVYGHSVLPFFYNPK
                     NYKTIFSTLGDLEKGDLIYVIYGNRSYTYEVEGQRELKPEEVNPIENIKPEYLRKSSL
                     VLMTCSPPGTKLKRLLIDSVLINTSIIQQNSIE"
BASE COUNT         2871 a         1740 c         1638 g         2529 t
ORIGIN      
        1 caacgtgatc ttttggttca aaatcaagct ttggaatgtc acccacatgc tttaatattt
       61 catttcctga ttcatctctt cctacaggaa cgtctgccga aggtatattt ggaacagcca
      121 taaccatagc gtccaattcg ttttttaaaa ccaacagttt atcttccaac ccctttagtt
      181 cctctttcgt gtcactagct tgtgtgatta atttttcacg agcatcgcca gatacttttg
      241 aaatgtcttg agagagctcg ttgcgaattg ctctaagctc ttctactttc ttaagtaaat
      301 ttctatactc aacgtcaact tgtagaagcc tatctatgtc cacaacgcca ttcatgtttt
      361 tgtcttctat tgcttttttt acaacctcag ggttttctct tattattttt atgtccaaca
      421 taatttattc ctaaaatata aataactact aaccggggat tgagaataaa aaaccccctg
      481 gttttaccca agaggctcaa tactaatgta ttgaaggtgc cagccttctt catacttatt
      541 aacagctata acgggcattc ccgacttggc atttcctcca agcagctccg tgattggaaa
      601 attcacatca aagctttttt agttttttta tacaaacaaa ctcaattatt tgttagtctg
      661 tgttcgtaaa tataacaatt aagtggtcga gattcaacca gtaaatactt tttttagcaa
      721 cgattgattg ctttacaggt ctgcaatcaa cactaagaaa tgatccggac gaaaaacctg
      781 ttcaaacggt aagagctggg gaagaagcgc agaaacttaa atgtttaatc cctatctatt
      841 tggcttgatt tttgtaaaca ttgatcaaaa actctacatg tgcatcaaaa ccctcaccta
      901 gttgttcaac accatataaa atctcttcac gatcaatttt agccgcaaaa gccctatctt
      961 taaatttctt ctttactgac gatactttca tgccgtcgta accttcgggg cgcatcaatg
     1021 cgtacgcata tagaaatccg gccatctcat ccacggcaat aagcgcagag gccagcctgg
     1081 tctgaggttt tacattggtt ctcatgtgag catgagctgc aactgcatga attaaactct
     1141 caggatagtt tctctcttta aaccattcta atgattttgc aggatgctca tcagggtatt
     1201 caaaataatc aaggtcatgt aataaacctg tgatatacca gagttcgcga tcttcattga
     1261 atttttcagc atatgcttca agaccgcggg cgatcataag agcatgctgt atttgatagc
     1321 tgtccttgac atggtcccgt aaaagcgcct ccgcctcctt tctagaaggt agaaatccct
     1381 cggagctaat gtataaactc tcgtttaaat cagttttact tagagagtct tctactagag
     1441 gatcgctctt ggataacccg tagatttcag cgtttgcatc gtcgtaaaca tgcttcaatt
     1501 gaggaaatgg aactccttcc cttgctgtaa catcctctaa aaaccaaaaa actctttctg
     1561 aatgaccata accaaatgtc ggcggcatgc cgtattcaag catttcaaca taatcaatat
     1621 cgagccactg cgcttccgaa tcaccggaat ctctcattga ctgctgttcc gaaaatcgtt
     1681 caaactgatc caagccacta tttatttccg accagccatt tccgagttcg gagccggcaa
     1741 taattggctg gaaacgctct gttattctgg tatcttttgg gttcgccttt gctaacggcg
     1801 acaaatactt agggtgatta attaaaaagg caggcccccc aatagttcta cgtatttctt
     1861 tccaaagagc gtccactaat cgtggccgat tctctgtctc tgcctttatt ccggcttttt
     1921 cgcaagctag ctttaaatct tcaatacttg aattgtgtac gtctactcca tatctctgct
     1981 tcatgatgtc agagaaatca atcctttccc actctttttt ggagaaatca acttcaaaac
     2041 ccctaatact gaacttcttc ttatctccgt aaagagattc aatcacgtaa ttaaaaaggt
     2101 cttgaataaa atccatacct gattgccaat cagcgtaagc ccaataaacc tccatcgcca
     2161 tgtgttcggg aagatgctcg tcggataaac cctcatttct gaatcttggg cctatttcat
     2221 aaactttttc atagccccct ccaattaatc ttttcaaaaa aagttcatgg gaaattctaa
     2281 gataaagagt ctcatcgatg gagtccatat gagtcacaaa aggattcgcg tctgcccctc
     2341 cgggcactgc ttctaaaaca ggaatattaa tctcatagaa accccttttt tcaaaaaact
     2401 cgcgatgagc agcccaaaac ttagaacgtc taatgtatct atcacgaaca tcatcattta
     2461 tccttgtatc tagatatctt ttacgcattc tggtctctct gtctttaagc ccatcgtgct
     2521 tgtcgggcaa tggtctaagg gatttggcca atattcttat gctgtggggc tctatagact
     2581 gctcgccgct atttgttatt actaaagctc ccttgcactc tatataatct ccaatatcta
     2641 ataaatccag ctcagagtag gatatttcac tatttttgtg ttcaactttt tcaagatttt
     2701 gttccttaat atatacctga attgtgcctg aaaaatcttt tatatcaaaa aatgtcagct
     2761 ttccatgtct tctcaacgac atgatccaac cggctaaaat tacttccttt cccccaagtt
     2821 tgccaaagct gcttataact tcagagatta ggtgggtcct aaaagacgtt gccggataag
     2881 gatcaacgcc tagatcttga agatttttaa gtttttgtaa tcttacatcc cttagctctt
     2941 ttaaagtggc cattatttta tttcctaaac tgaatacgta cttcgcaatt ataacagaaa
     3001 aggggcaggt acaaagcacc caaaaatagt gctaaaacat ttttacggcc taaatttttt
     3061 tatttgcttt tatattgcta acccgaacaa aattctttta gtacatggcc taacctgctt
     3121 atactagcca caaacaacaa agaactgtaa aatatctgca gagtatgaaa aaaataactg
     3181 aagctacgat cgctcaaggc actagggttt ttgtgagtac agatatagat gtacctatag
     3241 ataatgggca tattagaaat acttttagac tcgaccgtgg cttagaaact cttaagtaca
     3301 tagtagataa aggagcaatc ccagtaattg ggggccatat aaaaaatcct caaggaaaat
     3361 acgatcccca gcttagtaca gcacctctta aaaagtatta taatgaaaaa ttaggggagg
     3421 gttcttacga actattagaa aatctgcgtt ttgacattag agaagatcag aatgaggata
     3481 cctacgcaca ggaactggca aataaaacag aggtctttgt aaacgagaat tttgcaacct
     3541 cacatcgaac aaatgcttct ttagttgcta ttactaaata cttaccttca ttcgcgggac
     3601 ttaatttgag cagagaaatc gaagctttgg ataaaatact tatcgactac aaaaagcctt
     3661 ttactgcagt tattggcgga gcgaaagttg aaagcaaggc acctgttatt gattcgcttt
     3721 gcaacatagc tgacaatatc ctgcttggtg ggaaaatagg cttaaactat tctacggata
     3781 atgctagggt gagctctccg gccgactacg cagacggtaa tcaagatatc ggcccgaaaa
     3841 caatcctaaa ctatacagga tacatactgt cagcccaaac acttttatgg gccgggccta
     3901 tgggagctta tgaaaaagac gaattctgta ttggtacaaa tctaatcgca gatgcgatag
     3961 tggaagcaac aaaaggcggc gcttatagca taataggagg cggagatacg attacagctt
     4021 atagcaagaa ccacgcattg ggagaagtaa gttttgtttc aacaggagga ggggccatgc
     4081 tgacatatct ggcgcataag aatatgcccg gcattgaggc tttaggataa attatggcaa
     4141 aaagtttaaa agttcagttt ccggaacgtt gcataggctg cgaactgtgc atcatggaaa
     4201 tacaaagaca gcttaaaaaa cacggcctcg aaggttctct gattagagtt tttaaaagtg
     4261 ccaaagaagg aaaagaaata tgcgagactc tggaatactc aatagatcta gacccgagca
     4321 taaattcata cgacattgaa caaattagga aaatttgccc aaaaagtgtt tttgttgtgg
     4381 ccgacatgac agaagaagag aactacgaag acgctctgct ggaacagaaa gattaaatat
     4441 gcataaatca gtcttatttg ttaatttaaa aaataaaaca tacgaggtta aaaaatttga
     4501 cgaactcgca aaatacatag gaggagttgc tgttggattt aaacttcttc ttgataatat
     4561 agagcacgat ccaattatat tttctatcgg acctttgaac ggcgtgtttc ctttcgcatc
     4621 aaaaacttct atcgttctgc ataacaatgg cgtagtcgaa gacttatata tcgggggttc
     4681 cctaagctcc agaatggcct tttccggggt tgactccata gtatttatag aatccgcaga
     4741 agagcccaca tacgttgaaa taacgaatga ggaagtgttc tttaaaccaa taacaactga
     4801 tatagattct ttaggattac cggggaaaag atcagtttta aaaccaatta aaaataaata
     4861 ctttttagat gattattttt taactccgga gttatttttt gaagaaaagc ttgcaaagaa
     4921 aaatgtagga ggaatagtgt taacaggaac aaaaacacgt gaaataaaaa atcctgaaaa
     4981 atataaggaa cttttacacg aaatattagg taaaaagtct ttaatggaaa tagatgaggg
     5041 agatagcccc tcttgcacat tttgtccaat ggggtgctca aagtctcgta ccggagaaat
     5101 tggaggaaac atattggtac acagcatggt gggatgcaag cttagccaaa aaatatacag
     5161 cgacgtggga acaatttttt cttgcttgag tgtgttgggc tattcctaca cacacgaaga
     5221 tattgaagac ctgcctcttc tggtaaaaga ggtactggaa aaattaaatt aatgaaatat
     5281 ttagtagcaa actggaaatc taatatgaca gtctccgagg ttattgtctg gctaaaagct
     5341 tgggataaac taataaaaag cgcagagagt aatgatgcgg taagagtttt agtagcccct
     5401 tgttttatac atcttccatt atttaccggt ctggaaaata tttattcttg cgggcaggat
     5461 gtctcggaga acagcgtagg gccttatacc ggatacgtga atgctacaca gctaagggaa
     5521 ttcgctgatt acagcatagt cgggcacagc gaaagaaatg aagactctga aacagttgtc
     5581 aaaaagatta atcagtgtag aaataacgga attgaggcca ttgcttgttt cactcacact
     5641 gaaaatgcaa cagagtattc aaaattcaca aagatcttag cttgggagga ccctgaaaat
     5701 atatcaaaac aaggaaagta tgcagaatta aggtatggtg aaataaatca ctctattgaa
     5761 aaaatttact ctaatattga acccgatact gtgttattat acggaggctc tgttaactcg
     5821 aataatatta gcgaattaac tagaattaag agtatatcgg ggtttttagt aggaaacgca
     5881 agtctggacg tggctcactt ttataagcta tactgctcca tgatctagta caataaagtg
     5941 taagaagtta aaattaactt tctaaagaag tctttttaat agtagagagc taagagaaaa
     6001 atttaaaatg aaatacattg atgcgcataa aaaaataaat acaattacta aacctgtagc
     6061 taaaaaaaac tcaaaaggaa gcttcaaggg tattgctaca ctggcactta tacttctaat
     6121 cttcgttgcc gtcgggggaa cagttttact ttttaaaagc aagctgaacc ccgtttcaat
     6181 tgttgcaagc gcaacaaagg caaatatcaa agaaactgac gggagaacaa acattctagt
     6241 tctcggaagc gacaagagag ataacggcaa tgctgtcacg tcagtattga ccgacaccat
     6301 actgctagtc tcaataggaa gggtcgagca cgacgtacta ctactttcca tgcccagaga
     6361 tctgtatgtt gaaaattcaa agggatacag tacaaaaatt aacgctgtgt atactttaga
     6421 ggggccggag gaactccagt ctgtagttga agacgttctt ggtgttccaa ttcattacta
     6481 tgccgtagtt gactttaatt tgtttgtaaa tgccataaac gtacttggcg gcattgatgt
     6541 taatgttcaa aatgcgttca cagactacca gtatcctatt gagggtaagg agaacgcgcc
     6601 tattgaggaa agatacgaaa caatatcttt tgcggccgga aaacaacaca tggacggaga
     6661 gacagcattg aaattcgcga gatcaagaaa aggcgacaat aacgaaggta ctgatttcgc
     6721 aagagcagca agacaacagt tggtgattga ggcaattaag gaaaaggccc tatccttaaa
     6781 aacagttaca aacgcttcaa agattaaaga gttatacgac ctatacgcag aaggaataga
     6841 tacaaacctg gggtttgctg acgtccaggg cttctaccta ttgtcacaac aaatcaactt
     6901 tgacagcatc agatctattg ttctcgacga caggagcgag gcagataaag gtggtctact
     6961 ttatgctcca atcgatacgg ccctttacgg caatcaatat gttctcattc cgcagtccgg
     7021 agactatagc caaatacatg catatgttca aagatatctc tttggagaat aaaaatgatc
     7081 gttttagttc acggtaataa ctctgtaaac aaagccgatg tagtttttaa acttagtgaa
     7141 aaaatctcaa ccccaagaaa atatctttca attgacgaga attcaccaac agatgttcta
     7201 actgcaacag cgactaataa tatgtttacc ggtgcaccaa ttgtaattct ggatctgggt
     7261 tcaaatacaa aaagcgatgc aaagagtttc ctagatattt tcccgcgagt acctgcaaaa
     7321 agtttgctaa ttttattggc acaaaaagaa cttcctaagt caaatcaatt ccttcagcac
     7381 gcacagcaac aaggctataa aataatactt agcaacacta ctcccacagc aaacattttt
     7441 tcttttgtca gctccctata ctcaaaaaat agaaagaaaa catatagaga atacgagact
     7501 ttaatagcag aaggtgctga tcctttttac attctttcta tggtaatgtt cggcgcgaga
     7561 aagcaagcaa aagctgtgaa tggggataaa atagatgaaa aaacactatc tttatataaa
     7621 tatatcttcg agaccgacct aaaaacaaaa accggcaaaa ttcctgttga cgttgctctt
     7681 cctttgatca tagaaaaagt gctaacatct tagtgcatgg gaaaatttaa aaagaaggtt
     7741 taccacaaaa aatatactaa tgatcagatc tataacaacg taaatctggg atttacctta
     7801 aaaagctctg cggtcccact tgtacttaca gttacggcat gcgccatatt tgcaacccaa
     7861 gtactttacc cgcttataaa ttttacgacc caagatctta cgtccgtcgc cgctcaacac
     7921 acagctttag caaaaatatc aggttttcgc gagtttcaat actcagagtt acaaagaaat
     7981 gtactaggct caacaaataa tagcgaacag gtacaggaga agttttatat ttcgattcca
     8041 aaacttaata ttgaaaacgc tgaggtagag acaaactctt cgaacctaaa tcctgaaacc
     8101 gctcttggcc attatctggg ctcagcctta ccggggcaaa atggcacagc gtttgtgtac
     8161 ggacactctg tgctgccttt cttttacaac ccaaaaaact ataaaactat attttcaacc
     8221 ctgggagatc tagaaaaagg agatttgata tatgtcatat acggcaatag atcttataca
     8281 tacgaggtag aagggcagag agagcttaaa ccggaagagg ttaatcctat tgagaacata
     8341 aaacctgaat atctacgaaa atcgtcctta gttcttatga cctgctcacc gccgggaaca
     8401 aagcttaaaa ggctacttat agactctgtt ctaataaaca catcgataat acaacagaat
     8461 tcaatagaat agtgttattt actgacaata gcagatttaa tgagccaggt cttaaaatca
     8521 tccgccacca gcttacgcgg gtggtttgct gtaatttctt tgcgttgcaa acccaacatg
     8581 ttgggtaaac tatacctact agcttaagca agtagtttta agtaatgaga ttataaacaa
     8641 gaatacagaa atacgcctca tgggcgtatt cctaaaatgt tctataatta tagtgctctg
     8701 ggctcaacgc agatacctaa tctcttagaa tcttcttttc aaatctagtt ttaatatagc
     8761 tctttgggtt catgaaga
//
LOCUS       DAOSWN010000026         8486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_5332_length_8486_cov_4.496027, whole genome shotgun sequence.
ACCESSION   DAOSWN010000026 DAOSWN010000000
VERSION     DAOSWN010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8486
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5332_length_8486_cov_4.496027"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLF29_01955"
     CDS             <1..186
                     /locus_tag="PLF29_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52731.1"
                     /translation="PWGPRPGGPWGPGRGRGASQRQLLPPHEVSRYDALLLMPAPASI
                     GRLTPTGLLFGPSLGPR"
     gene            complement(711..890)
                     /locus_tag="PLF29_01960"
     CDS             complement(711..890)
                     /locus_tag="PLF29_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52732.1"
                     /translation="MSTKIRIAEYPSFIAENKKRSIRSDVEATTTERFLRLALLGQGN
                     NTYMLQDRRSKLLLC"
     gene            complement(919..1716)
                     /locus_tag="PLF29_01965"
     CDS             complement(919..1716)
                     /locus_tag="PLF29_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HXK52733.1"
                     /translation="MFISSLQNKTIKEVSKLKNKKYRDKALLVPVEELQSIAFAMQNG
                     FTIAKVFYCRSILNIKFGDLQALDMFVKIPCELTEVSQEVFMKITSAAKNPSGIIALV
                     KQRDASLVKHTPNFGDLYLAAEGIEQPYNLGALIRLADNVGAKGVFLCDVRADLYDPE
                     TVRSSLGTIFCLKIFRTTSVEVANWCRINDLKTCITSPGAKLSCFDTDLTKCSVVVVG
                     AEHGGLSKFWLDKADIKIKIPMYGQAESLSVSSSAAISLYELVRQKG"
     gene            complement(1770..2753)
                     /locus_tag="PLF29_01970"
     CDS             complement(1770..2753)
                     /locus_tag="PLF29_01970"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HXK52734.1"
                     /translation="MSPLIKLSEITKRRGKRGLKFIVRFTIGSAFFIVCLNFSNSVFF
                     NENPLFGVNFLAEVVISSVGALIGFFLIPDYFFKLRRWIEQLVSKIVFELVSDFWNQY
                     NQKMEMTRKNRAMNAKKKKNKQSNQKEADKVRGGTLLDTSVLIDGRILNIAKSGFITG
                     LLVVHKSVISELQLLADSEDVMKRKKGRRGLDALNELKKYTKVAVIRQNYKTNKVDDV
                     LVAAASECRLKIMTLDFNLKKIAEVQNIKVLNINELVEAVKPDYIPGDTLSIKIVQEG
                     KEKDQGVGYLDDGTMVVVSNSSDMVGSVVEVEVSKLIQTAAGKMVFCDKIK"
     gene            complement(2750..4087)
                     /gene="radA"
                     /locus_tag="PLF29_01975"
     CDS             complement(2750..4087)
                     /gene="radA"
                     /locus_tag="PLF29_01975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HXK52735.1"
                     /translation="MAKQKSVYICQSCSYQSPKWMGQCTECGEWNTFEESLVAQNNPR
                     GSKMNSGGVVKPFYLSQVKASQLNRYKIGILEFDRVLGGGFVPGQVILLAGEPGIGKS
                     TLLTQLAKTLQQDKSEVIYVCGEESPSQVKIRAERMDYSADNLLVMTETNTDTIVESV
                     RGACLGNKSVKLIIVDSIQTLSSSQLMGVSGSVGQVRLSAGMLSSLSKELGIPLIIVG
                     HVTKEGTVAGPKVLEHMVDTVLYLEGDNQHMFRVLKTTKNRFGPVSEVGLFEMREGGL
                     MEVLNPSELFLSNVGNSIPGSCVTVVMEGYRPLLFEVQALTSKTSFGYPRRTSSGYSN
                     NRLQVLIAVLENRCGINLSNHDVYVNVVGGLKIDDYGADLAVCLAIVSAVKGLALSKS
                     TAAFGEIGLLGEIRAVSNEGKRLSEAKKLGYTNALSSSSEKQLKVLISKQFKK"
     gene            complement(4246..6570)
                     /locus_tag="PLF29_01980"
     CDS             complement(4246..6570)
                     /locus_tag="PLF29_01980"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HXK52736.1"
                     /translation="MSLDRLTNRAKALITELSEGGDYVPALEIYKAIYSSSGLGGIIA
                     RQFPKIKIDKDLSINLNSLLKEAYYQASKMEHLYVGTEHLLLALLKIAKHKDFKTVAN
                     HLQSSNIFPKAMKMLSDKKENVILNSFGSNLNRTAVKKSSKQVVRKAELDKLIAILLK
                     KSNANPLIVGEYGVGKQSLVDSLVESINSLEVPTFLAGYQIYEFDLMAFASSILNKGG
                     LELSLSALQEELKGLNRAIIYIKNFQNLFISTSSGMGVPLIYPMFKDLLQNSGINFIA
                     TLHSSLYEKLAVDNSFILDSFTVLDVNEPSEEETIQILTHEARMLETFHGVRISSEVI
                     KYIYDSAKSLQMDTFFPQKGVDLLDASCAYVLVSENRVPKDYKDLVDQTVILYGKMDK
                     AVSAGTYEQALKIQTKLDSLEKRLLKYEKSMISSQEVEVTNVAVDETLRSLNLKKKEH
                     ELLDKDKLSNLYEQVRKEIIGQDQAVESVVKALIRSGLGLRPGNRPIGNFFFLGPTGV
                     GKTELAKVLAREAFEDDGKSHLIRLDMSDFSEKHTVARLVGAPPGYVGYSDGGELTSK
                     IESQPDSVVLFDEIEKAHPDVLNILLQIMEEGELVDAKGNTFEFDKAVIILTSNLGTE
                     ILHGREIGFKDSGSTASDSDVEKNLKQNLKKILKPELINRFDEIVVFKRLSKDDQMKI
                     LNLLLTKVNEQVREQDVTLHVYRSAKKHLLELGYSQEYGARSLRRVVEKELLDRVAEI
                     LLATKKRPIKISIRAHKGKLTATSTFQRKSTRKN"
     gene            complement(6576..7685)
                     /locus_tag="PLF29_01985"
     CDS             complement(6576..7685)
                     /locus_tag="PLF29_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HXK52737.1"
                     /translation="MPQARKISNPSPVMDSLLGKAGANLKQYAYGELVEGVVVSVTHS
                     EVLLDLGAKSEGIITGEELQDSDRSYKSLKVGDTVLAMVVQAENDQGYIVLSLKRAEK
                     DKKWKTIEDAFDTESVLEVQVLEYTKGGLLVDCLGLRGFVPLSHLDRVHFANDLAKTA
                     GGSETELKESLKVLAGKTLKAKVIEQDRLKNRLVLSEKDATAAYSDEAKKERLDNIKE
                     GDELDGIVTGVMPFGLFVDLEGVEGLVHISEIAWEKVSSPAMYYKVGSPIHVKVLGID
                     EESQKLALSVKRLIKNPWDGVEDRYPIGSRITGVVSKIVPFGAFVTLEKGLDGLIHVS
                     EAAGPLNVGDEVEAVITNVDGEKQKLALSTRLISN"
     gene            complement(7784..7892)
                     /gene="rrf"
                     /locus_tag="PLF29_01990"
     rRNA            complement(7784..7892)
                     /gene="rrf"
                     /locus_tag="PLF29_01990"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(7992..>8486)
                     /locus_tag="PLF29_01995"
     rRNA            complement(7992..>8486)
                     /locus_tag="PLF29_01995"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         2372 a         1946 c         1518 g         2650 t
ORIGIN      
        1 ccgtgggggc cgagaccggg ggggccgtgg gggccgggac gggggcgggg ggcgtcgcaa
       61 aggcaattac tgcctccgca tgaagtatcg cgttacgacg cgcttcttct aatgccagct
      121 cccgcgagta taggtcggct cacgccgacc ggacttctct ttgggccttc tctaggccct
      181 cgatgaggtc ttttccttgg aaaatcattc tactactctc cttgggcact gttgtgccca
      241 ttttttgccc ccgggggggg ctggtttact gcaggcaagt tgccagcatt tttttgtgct
      301 tgattttaac tcaagtccaa aaaaatgctg gcaacgaaaa ctgcttttta cgcagagtgc
      361 gcagaaccga aaggagtaga gtaatttata cagaacaatg ccgcgtctca accctagccc
      421 gaaaacccat tttatagcat tttaaggtgc tgttcagctg tttaatttaa actgctgatt
      481 caggcaaaaa tcagaagtta aactttatta tatgtattta ttgattatta gttgtgttta
      541 tttatagagg tattctataa taaaacgcct aatctgtcaa tactataggg ttatgatggc
      601 gaaactaaaa cctcaacgct aattgctaac acacaaattt tccctcttag caacgatgtt
      661 aagttcttaa ttatacaata cgggtcgctt gttattctta tgaaactgtc ctagcataat
      721 aataacttgc ttctcctgtc ctgaagcatg taggtattat taccttggcc taacaaggct
      781 aaacgtaaaa aacgctctgt ggttgtcgct tcaacgtcgc tccttatgga gcgtttttta
      841 ttttctgcaa taaaacttgg gtattctgca attctaattt ttgtagacac aataccttaa
      901 attcaattta cttaaattct agcctttttg tctaacaagt tcgtataagg agatagccgc
      961 ggaactggaa acacttaaag attcggcctg tccgtacatt ggaattttta tttttatgtc
     1021 ggctttatct aaccaaaatt ttgataaccc accgtgttca gcaccaacta caacaacaga
     1081 gcatttggtt aaatcggtgt cgaagcaact tagttttgca cccggagacg tgatacatgt
     1141 tttaagatca tttatcctac accaatttgc cacttctacc gaagtggtac gaaatatttt
     1201 taagcagaat atagttccca aactactgcg gactgtttcg gggtcatata aatctgccct
     1261 aacatcacaa agaaaaaccc ctttggcacc aacattatca gcaagtctta ttagagcgcc
     1321 taagttgtat ggctgctcaa tgccttcagc agcaagatac aaatctccaa agttaggggt
     1381 atgttttact aacgacgcgt ctctctgttt taccaaagct ataattcctg aaggattctt
     1441 tgcggcgctc gtgattttca taaaaacttc ttgagatacc tcagttagtt cgcacgggat
     1501 ctttacaaac atatccaagg cctgaagatc tccaaacttt atgtttaaaa tgctccggca
     1561 ataaaaaacc tttgctatag tgaagccgtt ctgcatagca aaagcaattg attgcaattc
     1621 ttccacaggt actagcaacg ccttgtctct atatttttta tttttgagtt ttgaaacctc
     1681 ttttatagtc ttattttgaa gactagatat aaacatgcgt gtattatata ccaaaaagcc
     1741 gatatttttg cttgaaatcc gcgataatgc tattttattt tgtcacaaaa aaccattttt
     1801 cctgcggcag tttgaatgag ctttgaaacc tcgacctcaa caactgagcc gaccatatct
     1861 gaactatttg aaacaaccac catagtgccg tcatctaagt aacctacccc ttgatccttc
     1921 tctttacctt cctgaacaat ttttatgctt aaggtatcgc ccggaatata gtccggttta
     1981 acagcctcga ccagctcatt aatgtttaaa acttttatat tttgtacttc agcgatcttt
     2041 ttaagattaa agtccagcgt cattatcttt agtctgcact cgctggcagc agcaactagc
     2101 acgtcatcta ccttatttgt cttataattt tgtcttataa cagcaacttt ggtatatttt
     2161 ttaagttcgt tcagcgcgtc cagcccccgc cttccctttt ttcttttcat tacatcttcg
     2221 ctgtcagcca acaattgtag ctcagatatg acgcttttat gaacaactag aagaccggtt
     2281 ataaaaccgc tcttcgctat gttcaaaata cgcccatcta tcaaaaccga agtatctaat
     2341 aaggtacctc ctctaacttt atcggcctct ttttggttac tttgcttatt cttttttttc
     2401 ttggcattca tggcccgatt ttttcttgtc atttccattt tttggttata ttgattccaa
     2461 aaatcagata ctaactcgaa tactattttt gaaactagtt gctcaatcca acgccgaagc
     2521 ttaaaaaagt aatcaggaat taaaaaaaag ccaatgagag cacctacgct cgatattaca
     2581 acttctgcta agaaattaac cccaaataaa gggttctcat taaaaaagac cgaattggaa
     2641 aaattcaagc agacaataaa aaacgccgaa cctattgtaa atcttacaat aaacttaagt
     2701 cctcttttgc ctcttctttt tgtaatttca cttaacttta ttagcgggct cattttttaa
     2761 actgtttcga aattaaaact ttcagctgtt tctcggaact cgaagacagt gcattagtat
     2821 agcccagctt ttttgcttcc gaaagtcttt tcccttcgtt agatactgct cttatttcac
     2881 caagcagccc gatctcgcca aaagcggccg tagatttaga taaagcaagg ccttttactg
     2941 cagacacaat agccaaacac actgctaaat cagctccgta atcgtcaatt ttcaacccgc
     3001 ccacaacatt tacgtataca tcgtgattag aaagatttat cccgcatcta ttctccaaca
     3061 ctgcgattaa cacttgcaac ctattattgc tataaccact tgaagttctt ctaggatagc
     3121 cgaaagacgt tttagaagtt aacgcctgca cctcaaaaag caaggggcgg tagccctcca
     3181 ttactacggt tacacaagag cccggtatgc tattccccac gttactcaaa aaaagctcag
     3241 agggatttaa tacttccata agaccgccct cacgcatctc aaataaaccg acctcagaaa
     3301 cagggccgaa cctgtttttt gtagtcttta atactctaaa catatgctgg ttatcgcctt
     3361 cgagatacaa cacggtgtca accatgtgct ccagaacctt agggcctgca accgtacctt
     3421 cttttgtaac atggcctaca ataattaacg gaataccaag ctccttagac aaagaggaca
     3481 gcatgccggc ggataaccta acctgcccca cagaccccga aacccccatc aactgggagc
     3541 tagaaagagt ttgtatagag tcgactatga tcagctttac agatttgtta ccaaggcacg
     3601 cgccacgaac tgactcaact atcgtatcag tgttggtttc ggtcatcact aataagttat
     3661 cggccgaata gtccattctc tcggctctta ttttgacctg agaggggctt tcctccccac
     3721 acacataaat aacctccgac ttgtcctgtt ggagtgtttt agccagctgt gtgagcagag
     3781 tgcttttacc aataccgggc tctccggcta ataaaatcac ctggcctgga acaaaacccc
     3841 cacctaaaac cctatcaaat tctaatattc ctatcttgta tctgtttagt tgagaggcct
     3901 ttacttgaga aaggtagaaa ggcttaacaa ccccacctga gttcattttc gaaccgcgag
     3961 gattgttttg tgcaacaaga ctttcttcaa aggtattcca ttcaccacac tcggtgcatt
     4021 gtcccatcca ttttggactc tgatatgaac agctttgaca gatgtaaact gatttctgtt
     4081 ttgccataaa ttaattgtac tatactaaga aaactattaa gtaaaagcgt ctagttaaat
     4141 aagttatcgt gctccagacg aaggctacaa taccgttata gatcagataa tggagcaaaa
     4201 aaataatttt ggactgtaat gacgaaaaaa aaccccagct agctgctaat ttttacgggt
     4261 gctttttctc tgaaaagtcg aggtagcggt taactttcct ttatgagcgc ggatagatat
     4321 tttgatcggc ctttttttag tggcaagtaa tatctccgct actctatcaa gtaattcttt
     4381 ttctacaaca cgtcttaggg aacgtgcccc atattcttga gagtatccta gctcaagcaa
     4441 atgcttcttt gcagagcgat aaacatgcaa agtaacatct tgctccctta cctgctcatt
     4501 aaccttagta agtagcaaat taagtatctt catttgatca tctttactca gtcgtttaaa
     4561 aaccacgatc tcatcaaacc tgttaattaa ctcgggcttt agtatttttt tgaggttttg
     4621 ttttaagttt ttttcaacat cactgtcgct tgctgtactt cctgaatctt taaagcctat
     4681 ttcacgacca tgaagtatct ctgtgccaag attgcttgtt aaaattatta cggccttatc
     4741 aaactcaaaa gtgtttcctt ttgcatcaac caactccccc tcctccatga tctgtagcag
     4801 aatatttaaa acatcagggt gggccttttc aatctcatca aacaaaacaa cgctgtcggg
     4861 ctgggattct atttttgatg taagctctcc accgtcactg tatcctacgt accccggggg
     4921 tgctccaacc agccttgcta ccgtgtgctt ttcgctaaaa tcactcatat ccaaccttat
     4981 caggtggctc ttaccgtcat cttcgaaagc ttccctcgcc aaaacttttg cgagttctgt
     5041 tttacctaca ccggtcggac caagaaaaaa gaagtttcca atcggcctgt ttccgggcct
     5101 aagacctaaa cccgatctta ttagggcctt aactacgctt tcgaccgcct gatcctgacc
     5161 aataatttct tttctcactt gctcataaag gtttgataat ttatctttat ctaataactc
     5221 gtgttctttt ttctttaaat tcagtgatcg cagagtctcg tcaactgcaa catttgttac
     5281 ctcgacctct tgcgaagaaa tcattgattt ttcatatttt aaaagtcttt tttcaagaga
     5341 gtccagcttg gtctgaattt tgagagcctg ctcataggta ccggcactaa cagctttatc
     5401 cattttccca tataaaataa cggtctgatc tacaagatct ttgtaatctt ttgggactct
     5461 gttttcggaa accaaaacat acgcgcacga cgcgtctaaa agatcaacgc ctttttgcgg
     5521 gaagaaggtg tccatctgca ggctttttgc actgtcataa atatatttta tgacttctga
     5581 ggatatcctt acaccgtgaa aagtttccag cattctggct tcatgggtta atatttgaat
     5641 tgtttcttcc tctgacggct cattaacatc caaaacggta aaactatcta aaataaagga
     5701 gttgtctacg gctagttttt cgtacaacga ggagtgcaaa gttgcaataa aattgatgcc
     5761 tgaattttgc aacaggtctt taaacatagg gtaaattaaa ggcacgccca tgcctgagct
     5821 tgttgaaata aagagatttt ggaaattttt tatataaata atagccctat ttaaaccttt
     5881 aagttcttct tgtaacgcag agaggcttag ctcaagacct cccttattta atatgcttga
     5941 agcaaaagcc ataagatcaa attcatatat ttgatagccc gctaaaaatg tcggtacttc
     6001 aagagagttg atactctcga ccaaagagtc gacaagagac tgcttcccca ccccatactc
     6061 tccgacaatt aaggggtttg cattgctctt tttgagtaaa atggcaataa gtttatcgag
     6121 ctcagctttt ctaaccacct gtttggaaga ttttttcacg gcggttctgt ttaagttaga
     6181 cccaaaggaa ttaagaatga cattctcctt tttatcggaa agcattttca tggctttagg
     6241 aaaaatattt gagctctgaa gatgatttgc aactgttttg aaatctttat gtttggctat
     6301 ctttaataat gctagaagta agtgctcagt acctacatac aaatgctcca tttttgaggc
     6361 ctggtagtaa gcttctttga gtagagagtt taggttaatt gataaatctt tatcgatttt
     6421 aatttttgga aactggcggg caatgatacc acccaagcca gatgagctgt agattgcttt
     6481 gtatatttct aaggcgggga cgtagtcccc gccttcagac aattctgtaa tcaaagcttt
     6541 tgctctgttt gttagcctat ctaatgacat agttactaat tagagatgag cctggtgctt
     6601 aatgcaagct tttgcttttc accatcaaca tttgtaatta ctgcctctac ctcatcgccc
     6661 acatttagtg gcccagcggc ctcggaaaca tgaattaaac catccaaacc tttctctaaa
     6721 gtgacgaaag caccaaaggg tactattttg ctaactacgc ctgttatcct tgatccaata
     6781 ggatatctgt cttctacacc atcccatggg tttttaatta atctcttaac gcttagagct
     6841 aatttttgag attcttcatc tatacctaac acttttacat gaatcggaga acctacttta
     6901 taatacatgg ccgggctgct aactttttcc caggcaattt cgcttatatg aaccaggccc
     6961 tctacgcctt ctaggtctac aaataagcca aaaggcataa ctccggtaac gataccatcc
     7021 agctcatcac cctctttaat gttgtcgagc cgttcttttt tggcctcgtc gctgtaagct
     7081 gcggttgcgt ctttttcaga taatacgagc ctatttttta atctgtcctg ctcaataact
     7141 ttagccttta atgtttttcc agctaaaact tttagtgatt ctttgagctc agtctcgctc
     7201 ccgcctgctg ttttagcaag gtcgtttgca aaatgcacgc gatcaagatg agatagaggc
     7261 acgaaacctc ttaagcctaa acagtcaaca agcaatccgc cttttgtata ctccagaacc
     7321 tgtacctcaa gtacagattc ggtatcaaaa gcgtcttcaa ttgttttcca ttttttatct
     7381 ttttctgctc tttttaacga gagcacaatg taaccttgat cgttttcagc ctgaacaacc
     7441 atggccaaaa cggtatcccc tactttaagg cttttgtagg atctatcaga gtcttgtaat
     7501 tcttctcctg taataatacc ttcggatttt gctccaaggt ctaataatac ttcgctgtga
     7561 gttacactta caacaacgcc ttcaactaac tctccgtaag cgtattgttt taaatttgct
     7621 ccagcttttc caagtaatga gtccattaca gggcttgggt ttgaaatttt tcttgcttgt
     7681 ggcataaaaa taaatgggtt tgcattttaa cttaatactt tagctcacta aaacgcataa
     7741 aaacgtgaaa tttatccaga aggataaaag tttgcgggca atgtcctggc gacgacctat
     7801 tttcccaatt aaatagtatc ttcggcgctg gtgggcttaa cttctctgtt cggaatggga
     7861 agaggtgttt ccccaccgca agtatcacca ggacactgac cacaaactaa atttagaata
     7921 cctacctaac agtaggtact ctaaaaactg aacaaaaaac aaatgtagac aacgcaacat
     7981 cttataagaa atataaaaag ttgcacacac attagtactg cttagctgaa tacattactg
     8041 tacttacacc tgcagcctat caacctggtc gtctcccagg aggtgcgtcc gcttacgcgg
     8101 aagtgttcta atcttggagt atgcttcgtg cttagatgct ttcagcactt atcaagaccg
     8161 aacgtagcta cccagcgatg ccactggcgt gacaactgga acactagagg ttcgtccgct
     8221 ccggtcctct cgtactagga gcagccctcc tcagaacaca tgcgctcgtt gtggaaaggg
     8281 accgaactgt ctcacgacgt tctgaaccca actaacgcgc ctttttaatg ggcgaacagc
     8341 ccaacccttg gaacctgctt cagctccagg ataagacgag tcgacatcga ggtgccaaac
     8401 cccgccgccg ctgtgaacgc tcgggcggga ttagcctgtt atccccgggg taacttttat
     8461 ccgataagtt ccaggcctac catgag
//
LOCUS       DAOSWN010000027         8239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_5597_length_8239_cov_4.271261, whole genome shotgun sequence.
ACCESSION   DAOSWN010000027 DAOSWN010000000
VERSION     DAOSWN010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8239
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5597_length_8239_cov_4.271261"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="PLF29_02000"
     CDS             <1..702
                     /locus_tag="PLF29_02000"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HXK52738.1"
                     /translation="GPDNMVQLGHDMGLSNWKVDNTYGLAVTLGGKEVRLVDLVGAFS
                     TFARSGRQRPISPFASIKDSNGFEIYNITRNYEKQVLSPQTSYIISNILSDNNARTPA
                     FGPRSQLVVPGHVVSVKTGTTNDIRDNWTIGYTPTYAVGVWVGNNDNRAMNRNLASGL
                     SGAAPIWNRVMGSVLKDIPDEPFIIPDGIVVKTDPSCGAIEVFDEKNPIPSRICPAPK
                     EKDSDKDKDKENKKD"
     gene            845..1300
                     /locus_tag="PLF29_02005"
     CDS             845..1300
                     /locus_tag="PLF29_02005"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HXK52739.1"
                     /translation="MKKVNFNDTKKKIMTSFAQVKKDIPNAEWRHLVTGVVIFALVVV
                     FSVSYFSKGNTPIDLSDLTDSEIMIENEATEVTAGEESMVDETTTDTTMAKVEAGEGE
                     TVVQKGEGLWHVAKRVCGAGEKYNKLAEANGMTIYSEVAEGQVLKVVCE"
     gene            1680..4649
                     /gene="uvrA"
                     /locus_tag="PLF29_02010"
     CDS             1680..4649
                     /gene="uvrA"
                     /locus_tag="PLF29_02010"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HXK52740.1"
                     /translation="MSSALDSIIVRGAREHNLKNIDLSVPKNKLVVFTGVSGSGKSSM
                     AMDTIYAEGQRRYVESLSAYARQFLGVMGKPDLDSIEGLSPAIAIDQKTTSKNPRSTV
                     GTITEVYDFMRLLYARVGHPHCPSCSREISRQSIDQISTQIVDILSSDKDFKTSKGVR
                     YFIFAPVVKDRKGEFNSLFANLKKQGLSKARIDNTLYDLSSDIHLIKTNKHSIEAVVL
                     RNIINKERLSTAEQKKDFQSRLHQTLETALRLANGLVELSIVKDSSFDFPEQPKEYED
                     HLFSENFACPHCNISLPEIEPRSFSFNSPHGACPSCDGLGTQLSVDEKSVLNGNLSIA
                     EGGIFPWTKLFEHNSWTSMVIKSVAEAYNFSLNTPIKDLPKDILELVLFGPKEERKFT
                     VNYRTKNNDSGTFQARYEGVIPNLIRRHKETSSDFIRNEIEKYMVKEPCPNCKGTRLK
                     DEILSIVIDGKNIANASDMSIKDFSGWTKQLESKFNERERKISQPILKEINYRIKFLL
                     DVGLGYLTLSRSSAHLSGGEAQRIRLASQIGTGLSGVLYVLDEPSIGLHARDQGKLIK
                     TLKHLRDLGNTVLVVEHDTDTMLNSDYIIDFGPGAGSRGGEVIAYGTPEEIMKNKNSI
                     TGAYISGKKKVGQTKIHNLSGTEEAVEKKYKTLSLKGASGRNLKNIDFEIPLNKFVCV
                     TGVSGSGKSTLIMDTLYTALRQYYGLKVEERPETFKTLSGQENIDKVVAIDQSPIGRT
                     PKSNPATYTKVFDEIRNLFAQTPEAKVRGYKPGRFSFNVKGGRCEACRGEGQIKIEMQ
                     FMPDVYVNCEVCEGRRYNREALEIYYKEKNISEVLEMTVEEAYEFFKNIPQIRRRLEV
                     LHDVGLGYIQLGQPAPTLSGGEAQRVKLSLELSKKARGNTLYILDEPTTGLHFKDLER
                     LVSIMKILISRGHSILVIEHNLDIISNAEWVIDLGPDGGDKGGEVVFSGSLQDLLKDK
                     KSYTAEALRKAIGQPGL"
     gene            complement(5143..5472)
                     /locus_tag="PLF29_02015"
                     /pseudo
     CDS             complement(5143..5472)
                     /locus_tag="PLF29_02015"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
     gene            6444..7802
                     /locus_tag="PLF29_02020"
     CDS             6444..7802
                     /locus_tag="PLF29_02020"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HXK52741.1"
                     /translation="MQTANLTEKLERLPKSPGVYLFLNNKSKVIYVGKAKYLPTRVKS
                     YFQQDVTLGTKTQRLVASIYDLDYIETRTELDALLLEAELIKRYKPRYNIALKDDKSY
                     VYIVIRNESVAVQARSRPVNIPKILVLRAQEIEKKDTVFGPFPEARVVRNTVRRLRRI
                     FPFRDCSISKFNKYSKMQKPCLYGHIGVCPAPCLHAGGLVAHQKSIVAMKKILSGQSN
                     RLLIEYKNLMQKASSEQRFEEAIEYRNTVSRLEYVSKNPSSATDYIQNPTLAEDSRQL
                     ALKSIALNIPLLNSPPVRIECYDISNISGKNAVGSMVVAIDGRIDKSEYRKFKIQTKT
                     TPDDFYMMSEVLYRRLKREISTQASSTKWGMPDLIVLDGGKGQVSAVLEVFTKLNIDI
                     PLIGIAKKFETLVYFKDGKFNELSLQKDDEGLKLIQQLRDEAHRFAQSYFHKLRLKEL
                     KS"
     gene            7799..8212
                     /locus_tag="PLF29_02025"
     CDS             7799..8212
                     /locus_tag="PLF29_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52742.1"
                     /translation="MIKKFITFFIYCSIGYYVASSTIGGIFISGNRVITSFFVVFVLT
                     FANMLAPHVFAILPLPKKGFSAIFLSLILNVAVFQVFIVFLPNFRIVASFLPELIIFG
                     FVLPSKHLNGIESLLCTALVFTITVKTFLWLGKER"
BASE COUNT         2506 a         1451 c         1737 g         2545 t
ORIGIN      
        1 ggcccagata atatggttca acttgggcac gatatgggtt taagcaactg gaaggtagat
       61 aatacatatg gcttagccgt aactttggga ggcaaggaag tgcgtctggt agacctagtt
      121 ggcgcattct ctacttttgc cagaagcggg cgacaaaggc ccataagccc cttcgcctct
      181 ataaaagatt caaacggatt tgagatctat aatattacaa gaaattacga gaagcaggtt
      241 ttgtccccgc aaacatcata tataatttca aacattttat ctgacaataa cgctcgtact
      301 cctgcttttg gccctagaag tcagctagtt gttcccgggc atgtagtttc agttaaaacc
      361 ggaacaacga atgatattag agacaactgg accatcggct atactccgac ctatgctgta
      421 ggagtttggg tcggaaataa tgataatcgg gccatgaacc gcaaccttgc atcaggcctt
      481 agcggggcag cacctatatg gaatagggtt atgggctcgg ttttaaaaga tattcctgat
      541 gaacctttca ttatacctga cggaatagta gttaaaacgg acccaagttg cggagcaata
      601 gaggtgtttg atgaaaaaaa cccgatacct tctagaattt gcccagctcc taaagaaaaa
      661 gattctgata aagataagga taaagaaaac aaaaaagatt agcttttatc ttaaaagcgt
      721 ttttaataac cgatctttga ttattacaat cgattttggt gtataaactt ttatggtcga
      781 gtttaaatct tgaatattaa agaaaatcat atattattat agtaaattaa ggaggtgagt
      841 actaatgaaa aaagttaatt ttaacgatac aaagaaaaaa ataatgactt cttttgcaca
      901 ggttaaaaaa gatatcccta acgctgaatg gaggcattta gtaacaggag tagtaatatt
      961 cgctcttgtg gttgttttta gtgtttccta tttctcaaaa ggcaacaccc caatagattt
     1021 aagcgatctt accgattccg aaattatgat agagaatgaa gccaccgaag ttacagccgg
     1081 cgaagagtct atggttgatg aaacaacaac tgacacaaca atggcaaagg tcgaagctgg
     1141 agaaggcgag actgtagtac agaaaggtga aggtttgtgg cacgttgcta agagagtgtg
     1201 tggtgcaggt gaaaaatata acaaactggc tgaagctaat ggcatgacca tttattcaga
     1261 ggtcgctgaa ggtcaagtgc taaaagtagt ttgtgagtaa ttattatatt gcaatagaat
     1321 aataacacta ggttgttatt acgttacccc tatacccctg cccaaggggt atattttttg
     1381 tctcgtaccc gttctcaact ttataatttc gttactcaaa cctatttacg tgggctagta
     1441 gatttagttt gcccaacatt ttgggttttg cagtgcaaag aaattacatt aaaccgccag
     1501 cgtaagctgg cggtggttga tttctgcagc gggaaggccc cttgctttag ttcgcggtga
     1561 aagtttatgc gtggagcagt aggggcgagg ctacggactt atgtccgtag tctcttcatt
     1621 gtaaaactca aaatacagag tgttgaaata cactcaattg tttcgtataa tctttgggta
     1681 tgtcttcagc tctagatagc attattgtta gaggtgcccg agaacacaat ctaaaaaata
     1741 tcgatctcag cgttcctaaa aataagttag tagtatttac cggtgtttct ggttccggta
     1801 agtcttctat ggccatggac acaatttatg ctgaagggca gaggagatat gtcgaaagcc
     1861 tatcggccta tgccagacag ttcttggggg ttatgggaaa gcctgacctg gacagtattg
     1921 aaggcttgtc tcctgcaatt gcaattgatc aaaagacaac ctctaagaat ccaagatcta
     1981 ctgtaggtac cataaccgaa gtatatgact ttatgcgttt gttgtacgct cgtgtcggcc
     2041 accctcattg cccttcttgt agccgtgaaa taagcaggca gagcattgat caaatttcta
     2101 ctcaaatagt agatatatta tcttccgaca aagactttaa aacttcaaag ggtgttagat
     2161 attttatctt tgcccctgtt gttaaagatc gcaaaggaga gttcaactct ttgtttgcaa
     2221 atttgaaaaa gcaaggtttg tctaaagcta gaatcgataa taccttatat gaccttagtt
     2281 cggatattca tttaattaag acaaataagc attctattga agctgtggtt ttgagaaaca
     2341 ttatcaataa agaacgtttg agtacagctg aacagaaaaa agattttcaa agtcgccttc
     2401 atcaaactct tgagactgcg ctgaggctcg caaatggttt ggttgaactc tcaatcgtta
     2461 aagatagttc ttttgatttc cccgagcagc cgaaagaata tgaagaccat ttattttctg
     2521 aaaattttgc ttgcccccac tgtaatatat ctttgcccga aattgagcca agaagttttt
     2581 cttttaactc tccgcatggt gcgtgtcctt cgtgcgacgg tttaggaact cagctaagtg
     2641 ttgatgaaaa atctgtatta aacggcaatc tctctatcgc agagggagga atatttccct
     2701 ggacaaaact attcgagcat aattcctgga cctctatggt cattaaatca gttgccgaag
     2761 cttataattt ttctctcaac acgccaataa aagatttacc taaagatatt ctggagcttg
     2821 ttctttttgg accaaaagaa gaacgtaaat ttactgtaaa ctatagaacc aaaaataatg
     2881 actcaggcac ctttcaggct cgatacgaag gggtcattcc taatttaata aggagacata
     2941 aagaaacctc ctcggatttt attagaaatg agatagaaaa gtatatggtt aaagaaccat
     3001 gcccaaattg caaaggcaca agattaaaag atgagattct ctctatcgtt attgatggta
     3061 aaaatattgc gaacgcctca gacatgtcga taaaagattt ctcgggctgg acaaaacaac
     3121 tcgagtccaa atttaatgag cgggagagaa aaatttcaca gccaatttta aaagaaataa
     3181 actacaggat taaattcctt ttggacgtag gcttggggta tctgacattg agtcggtctt
     3241 ctgcacattt gtcgggaggg gaggctcaga ggattcgtct tgcttcccaa ataggtacag
     3301 ggctttccgg cgtgctgtat gtacttgacg agccttccat tggcttacac gcacgtgatc
     3361 aaggaaaact tattaagacc ctgaaacact taagagatct tggtaacaca gttttagttg
     3421 ttgaacacga cacggacaca atgctgaata gtgattatat tatcgatttt ggtcctggag
     3481 cgggatcgag aggaggcgag gttattgctt atggtacgcc cgaagagatc atgaaaaata
     3541 aaaactcaat aaccggagcc tatatctcag gtaagaagaa agttgggcag acaaaaatcc
     3601 ataatcttag tggtacagag gaggcagttg agaaaaaata taaaacttta agtttgaaag
     3661 gagcatcggg tagaaatcta aaaaatattg attttgaaat cccattaaat aagtttgttt
     3721 gtgttaccgg tgtgtcaggg tctgggaaat cgactctaat tatggacact ttgtacactg
     3781 cgctaaggca atactacgga cttaaggttg aggaaaggcc ggaaaccttt aaaacactat
     3841 ccgggcagga gaacatagat aaggtagtag ccatcgacca gtcacccatc ggaagaactc
     3901 ctaagtctaa tcctgctact tatacaaaag tttttgacga aataagaaat ttatttgctc
     3961 aaactccgga agcaaaggtt cgaggatata agcccggacg tttcagcttt aatgttaaag
     4021 gtgggaggtg tgaagcgtgc agaggtgagg gccagattaa aattgagatg caattcatgc
     4081 ccgacgtgta tgttaattgc gaggtttgcg agggacgaag gtataatcgc gaggctcttg
     4141 agatctatta taaagaaaaa aatattagtg aagttctgga gatgactgtt gaagaagctt
     4201 acgagttttt caaaaatatt cctcaaatta gacgaaggct agaggtttta catgatgttg
     4261 ggctcggcta tatccaactg ggccagcccg cgccaacact ttccgggggt gaggcacaga
     4321 gagttaaatt atccttagag ctttcaaaaa aggctcgcgg caatactttg tatatactcg
     4381 acgagcctac taccggactt cacttcaaag atcttgaacg gcttgtaagt attatgaaga
     4441 tactcatatc ccgaggacat tcaattttag ttatagaaca taatctggac attatttcga
     4501 acgccgagtg ggtaatagat ttaggacctg atggaggaga taagggcggc gaggtagttt
     4561 tttccggttc tttacaagac ttactaaaag ataagaaatc atacacagcg gaagcgctaa
     4621 gaaaagcaat aggacaacca ggtctttagc taatagcgcg taagagactt ttggtgcata
     4681 actcaaagat attattttcc cggtcggctc tcgaagcgtg atacttgagt atcctttttt
     4741 aatataaatc gttttggtct gattttgtaa gatggtcgtg tttttttgtt tggaaatcac
     4801 atctaaagga tgcagagcca cgtttcctgt gtttgaaaga taagctattc tctcggatcc
     4861 taaagtgaaa ggattaaaga gaatagattt gtatacaaca aaattcggtt caaacgtgcc
     4921 tttagttctt ataggttctg tagtttttgt agatacctcg gattcagtat caaaaaagat
     4981 cttagaggct tgctcggtat atccttggtt tagattgcta ggcatctttg gaagagagag
     5041 gtttaaatta tagtcttttc ttattatcgc gaagtgatcc gcatttaacc ttgcaaagct
     5101 gtctatcgaa gtggtttttg caagtgccgt atctacttga acccatccaa gttttgggtc
     5161 aaatatttcc gcccaagtat gtggtccggt gtgggcttcc cctttagcat ccgaggtcag
     5221 caccaggccg acaacctctc ttgccggaat gcctgccgaa cggcatagag caataaaaag
     5281 gtcatttagg tcagtggcgg tgcctgcttc cccttctttt aatgttagag cggctccttt
     5341 tcgtggtttt atttcggttt cggagtattc atatgaaata ttggtcagaa tgtatttata
     5401 aatctcgtac gcgttcttca caactgattg cgaggtgttt agaatctgct ccgctgtctc
     5461 ggaaagttcc atgtcttttg tttgccagtt ctcattttgt ttagtgtatt tttttgtgct
     5521 ttttctgatt tcagaaaaag cacctccttg agttaaatca agttttcgac ttattatttg
     5581 ggcacttcct tggagtataa tattaagctc ctcccctgat ttaaggctat attcaccata
     5641 agaattgccg tgttcatcca gagctactct tttaggttcc gggtctatac tagcaatcgc
     5701 tatctgctgc gcgtcgataa tatctgaggg aatgacgata ctttttttag tatcaaattt
     5761 ggagtcattc tttatactat aaaccagtgt gaagtttatt aactgagtgt ttccaaaagt
     5821 tcctcctatc ccaaagttct gcaattgcgc tagtgagtat ttatataaag tgctgttttc
     5881 tttgtatata atgctttccg gtgcaggcga tataaatatc tccttaccat aggaagaggg
     5941 aatctctaaa gtagtttgat attctgctgt ttcacttgga agattcaccc taggtacact
     6001 tatgtgccat atagagccga ttttttctgc catgctggtt gtcttgtatt gaactatgag
     6061 tttattctct ttcccttctc ctataagaaa gttttttagt tcgattttta ttctattttc
     6121 ctgcccgact ttttcaactt cggtcttcaa ttcatctttg ttttgatcaa atgcctcaac
     6181 ttgcgctacc tcaatatccc gaacaattat tgtgtaggtt tttggtatta cactgtcttg
     6241 catatttatt atggatatct cttgttttac tgacgtttcg cctgtttctt caaaaatgta
     6301 tgatgtgttg aatttaagtg cgaattcttg aattgaggtg ttttgcgcat gtacattctt
     6361 aaacgaaaga acacctaaaa atatgcagga gaagataagt gaaaaaaata attttttcat
     6421 tacttcaatg ttagaataaa taggtgcaga cagcaaattt aactgaaaag ctcgagaggt
     6481 tgcctaagag tcctggggtg tatctttttt taaataataa atcaaaagta atatatgtgg
     6541 gaaaagcaaa atatctcccg accagagtaa aaagctattt ccagcaagat gttactttgg
     6601 gcacgaaaac gcaaagactt gtagcaagca tttatgacct cgattatatt gaaacaagaa
     6661 ccgagctcga tgctcttctt ctcgaggcag agttaataaa aagatataaa cccagatata
     6721 atattgcgct aaaggacgat aagtcatacg tgtatatcgt tataaggaac gagagtgttg
     6781 cagtccaagc tcgttcgagg cctgtcaata ttccaaaaat tcttgttctt agagcacaag
     6841 aaatagaaaa aaaagacacc gtattcggtc catttcctga agccagggta gttagaaata
     6901 ctgttagaag attaaggagg atctttcctt ttagagactg ctccatatca aaatttaata
     6961 aatattcaaa aatgcaaaaa ccttgtctat atgggcatat cggtgtttgt cctgcgccgt
     7021 gcttacatgc tgggggtttg gtagcgcacc aaaaaagcat tgttgcaatg aaaaaaattc
     7081 tctcagggca aagtaaccga cttttgattg agtacaaaaa tctaatgcag aaagcttctt
     7141 ctgaacaaag atttgaggaa gctatagaat acagaaacac tgtttcccgc ctagagtatg
     7201 tttcaaaaaa tccttcttcg gcgaccgact acatacaaaa cccaactttg gctgaagatt
     7261 caaggcagtt ggcattaaaa agcattgctt tgaacattcc tctactgaac agtcctcctg
     7321 tacgtattga atgctatgat atctctaata tttcggggaa aaatgctgtg gggtcgatgg
     7381 tagttgctat tgatgggcgt atagataaat ccgaatatag aaaatttaaa atacaaacaa
     7441 aaacaacgcc tgacgatttt tacatgatgt ccgaagttct ttacaggagg ctaaaaagag
     7501 agatttcaac acaagctagc tcgaccaagt ggggcatgcc ggatttgata gtcttggatg
     7561 ggggaaaagg tcaggtttcg gctgtattgg aagtttttac aaaattaaac atcgatattc
     7621 cgcttattgg aattgcaaaa aaatttgaaa cactggttta ttttaaagac ggtaagttta
     7681 acgagctttc cttgcaaaag gacgatgagg gtttaaaatt aattcaacaa cttagagatg
     7741 aggctcatag gtttgcgcag agctattttc ataaattgcg ccttaaagag ttgaaatcat
     7801 gataaaaaaa tttattacat tctttattta ttgctccatt ggttattacg tcgcgagcag
     7861 tactataggg ggaatattta tatcaggtaa tagagtaatc acgtcttttt ttgttgtttt
     7921 tgtgctaact tttgctaaca tgcttgcgcc tcatgtattt gctattttac cgcttccaaa
     7981 aaaagggttc tctgctatat ttttgtcttt aattcttaat gtagctgttt ttcaagtttt
     8041 tattgtattt ttgcctaact tcagaatcgt tgcttctttt ttgcctgagt tgattatttt
     8101 tggctttgtg ttaccatcta agcacctaaa tggaatagag tctttgttgt gtactgctct
     8161 ggtttttaca ataacggtca aaacattttt atggttaggc aaggaaagat agtattatta
     8221 taaaatatgt taaatgtat
//
LOCUS       DAOSWN010000028         8222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_5618_length_8222_cov_3.739439, whole genome shotgun sequence.
ACCESSION   DAOSWN010000028 DAOSWN010000000
VERSION     DAOSWN010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8222
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5618_length_8222_cov_3.739439"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1292
                     /locus_tag="PLF29_02030"
     CDS             <1..1292
                     /locus_tag="PLF29_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HXK52743.1"
                     /translation="IDLRDTTGVVQCVGVKIMSELGTEWVVELVGVVKARPDNMLNDL
                     MPTGTIELEVLEYSVLNKCKELPIPINTDGKEINEESRLKYRYLDLRRQRMAKIIKLR
                     SKLNGEFRKALEEHRFIEVETPMLTKSTKEGARDFIVPSRHNPGKFYALPQSPQQYKQ
                     LLMTAGVERYFQFARCIRDEDLRADRGYEFTQLDLEMSFVSQEEIMSAVEDIVKKAVT
                     SVGGKLKESTFPVINYYDALNKYGADKFDLRTDTEKKEGVLAFAWVINFPFFKKVDTS
                     DAAEVQDGKSGWTFTHNPFSSPIPEHVEWHLQQKNIDKIVTAQYDLVCNGYEVGGGSV
                     RAHNPEILKATFKIMGYSDAEIHEGVGHMLEAFELGTPPHGGIALGVDRLVIILAGED
                     SLKESIAFPMTSSGRTAVMDAPSEVSEKQLKELHIKL"
     gene            1334..1786
                     /locus_tag="PLF29_02035"
     CDS             1334..1786
                     /locus_tag="PLF29_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52744.1"
                     /translation="MSSYTDGAKSGKAEQLGVADLYSFFQRYAEGETVTQDILFPPLY
                     VDRELLFCRIISDESGPRLRVLKFTGETYDYSISSRGEGELVLMSALGPNSSGVIAYA
                     SQCAIYRRGAEAGENSAHLTNFLYAHKNNPQTVADQFSTKAASEEIQR"
     gene            complement(1789..2385)
                     /locus_tag="PLF29_02040"
     CDS             complement(1789..2385)
                     /locus_tag="PLF29_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52745.1"
                     /translation="MKMFKKIALISVVFFLLGSVIFLNLSFFYNYRENSKIKKVTQTT
                     EQAIPTNISETSIKKTDIPEDDGMSFELRFSEEFTQKGSVSGRLGYPGENIPQLEVYF
                     INQDSKNKNVTIITSYNQSEYFLQGLADGKYIAVAYVKDQPETSGAYTEAVLCGLTIE
                     CTDHSPVVFEIKDGNAAKNIDITDWYAPKGVLPEINSF"
     gene            complement(2385..3095)
                     /locus_tag="PLF29_02045"
     CDS             complement(2385..3095)
                     /locus_tag="PLF29_02045"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HXK52746.1"
                     /translation="MLTLLQYRTDQSGWHEVKCMYSANMCKYNEMQIVNVANPHITVQ
                     DMLPLLSKSTQVIIGGLGESGFEEKNPKKKALFKGMVEKSKPLLSHIIEREIPLLGVC
                     FGHQLIAHLYGGEVVEDKSKAETGLNKITLNANGLNDPIFSGIDKDFYAIEGHKSSVV
                     KLPIGAALLASSEKCEIQAYRLGRATYGLQFHSELDVEDVNYRLSLYPEYAGNSLNIK
                     YNKELVVKKILKNFAILS"
     gene            complement(3197..3271)
                     /locus_tag="PLF29_02050"
     tRNA            complement(3197..3271)
                     /locus_tag="PLF29_02050"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3237..3239),aa:Gly,seq:gcc)
     gene            complement(3446..4156)
                     /locus_tag="PLF29_02055"
     CDS             complement(3446..4156)
                     /locus_tag="PLF29_02055"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXK52747.1"
                     /translation="MPNKYKKPEYTKYFPKMPMYKAALLTDEMTGLPNRVGAIHIISS
                     LLKNHPQIAIYFFDINHQKDINHYLGREPVGCGTIATVCAVQRIKASSRNAIVSRLSG
                     DEGIVITTNPLISIEDEAYYKELDLPWVDYETATIMGEKVREYASGIPYNFSPEVFDN
                     AKREDINIFNTIFPPTVSVAGFTVSISELLENSVNELGNILWAKVSRADEEASKSKKI
                     NYSSYSEAIKDKLLRKLS"
     gene            complement(4180..4254)
                     /locus_tag="PLF29_02060"
     tRNA            complement(4180..4254)
                     /locus_tag="PLF29_02060"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4220..4222),aa:Gly,seq:ccc)
     gene            complement(4363..4448)
                     /locus_tag="PLF29_02065"
     tRNA            complement(4363..4448)
                     /locus_tag="PLF29_02065"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4413..4415),aa:Leu,seq:gag)
     gene            complement(4556..4643)
                     /locus_tag="PLF29_02070"
     tRNA            complement(4556..4643)
                     /locus_tag="PLF29_02070"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4607..4609),aa:Leu,seq:cag)
     gene            complement(4712..5452)
                     /locus_tag="PLF29_02075"
     CDS             complement(4712..5452)
                     /locus_tag="PLF29_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HXK52748.1"
                     /translation="MKNNKPTIIVLSLVILLSLVFVVYSLTNKEKHKATKDDRYDPNV
                     MKQINIPATSELGYNKNNALPDDAVLYLISDATAGYRANKMFLNKPGEQIEGSAELTD
                     VLIWVTSDDRMLYANASLSIDMLKSPSAQRDSDILKIFKDSSVLISVNGFQLPEDYES
                     GQFSFSIPVMLEINSVKKEVIFDVNAVTNDSTFDVSGEATVLMSDFGVEAPSLLNVFN
                     VDDSIILKFQIQGMPESSIDIEDPVSGL"
     gene            5489..6022
                     /locus_tag="PLF29_02080"
     CDS             5489..6022
                     /locus_tag="PLF29_02080"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXK52749.1"
                     /translation="MQKNIVIVGAGFAGLYTAKYLEKRLKLLKSKKIEASIALIDQNN
                     YFTFTPLLHEVASGGLERELILHPIREAFGTGVSFINSKVERVHLGKNFVMTSDKIQL
                     PYDYLVLATGSVSSLKYDVLNTEIFKLKTVNDAYNIKSAILTVFERKQNSLLNPPDKS
                     RYCWWGTYRCRTRCRAG"
     gene            5925..6704
                     /locus_tag="PLF29_02085"
     CDS             5925..6704
                     /locus_tag="PLF29_02085"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXK52750.1"
                     /translation="MNENKIACSTPPIKVVIVGGGPTGVELVAELAEYMHGTLARLYK
                     HVGPGQNQILLLNSAGHLLGSFDKRLGERIEKYLVQKLNVKVINNSKIISIEDFVIKS
                     SNGEIRGADVIIDASGVRAMYPDLIPAIDTDEKNNIITDDFLRIPEYPNVYALGDVGQ
                     FYKLAQTAVNQAEYAAKSIVNLMLNKQPKQYTFKPKGFLLSLGKNYAIGSLFNVLIFG
                     KPVWFLWRTIYLFKVLNSRHRIKIALNWTLNLFSDRDTTNI"
     gene            6701..7801
                     /locus_tag="PLF29_02090"
     CDS             6701..7801
                     /locus_tag="PLF29_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HXK52751.1"
                     /translation="MNKQFLQKLTAFQNSFNANFFLEIEDLEKHMMENMSHSLQEVYR
                     RYNKLSTNGGKRIRPFLADVSYSAFGGQDTKNIRKLCIAVELTNSYILALDDDLDRTQ
                     LRRNSPTANKSIYEYLLTLRPTTEMSYAHHLTSSLISITSLMYMHYINSFIIEQTTFE
                     ESVKLRLIDTLAKMIFKTAQGENSETVLMLDRDISIKRSLDILLTKTGYYTIETPVRM
                     GYFATGEENPSVTSSLEEYAHNVGLAFQLNDDIIGIFGDTNIAGKSNSDDIKEGKITP
                     LVAYIYENGSSKDISVIESILGRADITDEEADRFRDVVVSSGSKDFVSNLAREYADKG
                     KASIKNAEKFAVSKDAFNTFFELADFVIDRSF"
     gene            complement(7858..>8222)
                     /locus_tag="PLF29_02095"
     CDS             complement(7858..>8222)
                     /locus_tag="PLF29_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HXK52752.1"
                     /translation="DIQEDARRGRIYLSTTDMGSFGVTREQIFSGSFSNELHDLMLDY
                     ANKTVCFYKEGIKGADLLPKKTRYSILLSAHLYFQILVKLKKRDMNPFLGRVYTKTQE
                     KILIALLLLIYRRKSFKL"
BASE COUNT         2555 a         1534 c         1627 g         2506 t
ORIGIN      
        1 ttatagatct tcgagataca actggtgttg ttcagtgtgt aggcgtgaag attatgtctg
       61 agcttggaac tgagtgggtt gttgagcttg ttggggttgt caaggctcgc cctgacaaca
      121 tgcttaatga tttaatgccg acgggcacta tagagcttga agttttagaa tatagtgttc
      181 taaataaatg caaagaactc cccatcccaa ttaatacaga cggtaaagag attaatgaag
      241 agagcaggtt aaaatacaga tacctcgatc ttagaagaca aaggatggca aaaatcatta
      301 aactaagatc taagctaaac ggcgagttta gaaaggcact agaagaacac aggtttattg
      361 aggtagagac cccaatgctt actaaatcaa ccaaggaagg tgcaagggac ttcattgttc
      421 cttcaaggca taatcccggc aaattttacg cacttccaca aagccctcag cagtataaac
      481 agcttttaat gacggcaggc gtagaaagat acttccagtt tgctagatgt ataagagacg
      541 aggacctaag agccgacaga ggctatgagt ttacacagct tgaccttgaa atgagctttg
      601 tatcacaaga agaaataatg tcagcagtag aagatatagt aaagaaagcc gtgacttcag
      661 tgggcggaaa gctaaaagaa tccacctttc ctgttataaa ttactacgac gccttaaata
      721 agtatggggc agataaattt gatttaagga cagacacaga aaagaaggaa ggcgttttgg
      781 ctttcgcatg ggtcataaac ttccccttct ttaagaaagt agatacctct gacgcagcag
      841 aagttcagga cggaaaaagt gggtggactt ttactcataa tccattcagc tctccaatac
      901 cagaacacgt agagtggcac cttcagcaaa aaaatataga taagatagta actgctcaat
      961 atgatcttgt ttgtaatggg tacgaggttg gtggcggaag tgttcgagca cataacccag
     1021 aaattcttaa agcaaccttc aaaattatgg gttattctga cgcagagatc catgaggggg
     1081 ttgggcacat gttagaggcg ttcgaactgg gcactcctcc ccacggaggg atagctttag
     1141 gtgtggacag gcttgtaata attcttgccg gagaagactc gctaaaggag tctatagctt
     1201 ttccaatgac atcctcagga cgtactgctg taatggacgc gcccagcgaa gtgagtgaaa
     1261 aacagctaaa agaactgcac ataaagctat aagaatagtt gccacattta acttatagtg
     1321 gtaaaatttg gcaatgagtt cttacacaga tggagcaaaa agcggaaaag cagagcagct
     1381 tggcgttgca gacctttaca gtttttttca aaggtacgca gagggcgaaa cagttaccca
     1441 agatattctt tttcctcctc tgtatgtaga tcgagaactt cttttttgta gaataatatc
     1501 agatgaaagt ggcccccgcc ttcgtgtttt aaaatttact ggagaaacat atgactatag
     1561 catttcaagt cgaggcgagg gcgaacttgt attaatgagc gcgctcggcc caaattcatc
     1621 tggagttatt gcatacgcaa gtcaatgtgc aatatataga agaggcgcag aagcgggaga
     1681 aaatagcgct catcttacaa attttttata tgctcataag aataatccgc aaaccgttgc
     1741 ggatcaattc tccacaaaag cggcatcaga agaaatacaa agataaattt aaaaactatt
     1801 tatctcaggt aaaactcctt tcggggcata ccaatctgta atatcgatgt ttttagcggc
     1861 gttgccatct tttatttcaa aaacaaccgg agaatggtcc gtacattcaa tagtaagacc
     1921 acatagtaca gcttctgtat acgcgcctga ggtttccggt tgatctttta cataagcaac
     1981 cgcaatgtat ttaccgtctg caagtccttg taaaaaatac tcactttggt tgtagctggt
     2041 tatgatggtt acatttttgt ttttgctgtc ttgattaata aaataaacct caagttgggg
     2101 gatgttttcg ccgggataac ctagccggcc cgatactgaa cccttttgag tgaattcctc
     2161 tgaaaacctt agttcaaagg acatgccatc atcttcagga atgtccgttt ttttgatgct
     2221 tgtttcggaa atatttgtgg gtatagcttg ttctgttgtt tgtgttacct ttttgatctt
     2281 agaattttcc ctatagttgt aaaagaaaga taaattaaga aatattactg agcctagaag
     2341 aaaaaaaaca acgcttataa gtgcgatttt cttaaacatt ttcattaaga tagtatagca
     2401 aagtttttaa gtattttctt aaccacgagc tctttgttgt attttatgtt tagtgagttt
     2461 ccggcatatt cagggtaaag agaaagccta taatttacgt cttccacatc aagctctgag
     2521 tgaaattgca ggccgtaggt agcacgacct aatctgtatg cctgtatttc gcacttttca
     2581 ctagaggcaa gtaacgctgc tcctatagga agtttaacaa ccgatgattt atgcccctca
     2641 atggcataaa agtctttatc aatgcccgaa aatatcgggt cattcagacc attcgcgttc
     2701 agcgttattt tattgagccc ggtctccgct tttgatttat cctccacaac ttccccgcca
     2761 tataaatgcg ctattagctg gtgtccaaaa caaaccccca ataaaggaat ttccctttca
     2821 ataatatgcg atagaagtgg ttttgatttt tctaccatac ccttaaataa agctttcttt
     2881 ttaggatttt tttcttcaaa tcccgattcg ccgagtccgc caataattac ttgggttgat
     2941 tttgaaagca aggggagcat atcttggacc gtaatgtggg ggtttgcaac attgacaatt
     3001 tgcatttcat tatacttaca catgtttgcg gaatacatac atttgacttc gtgccatcct
     3061 gactgatcgg ttctgtattg aagcagtgtt agcatattaa ggattatagc aaagttttgt
     3121 tgttacttaa atctatagca agagaaaaac agatcgagtt catttctttt cttaagtgtt
     3181 tattttgggt gtaaggtgga gcgagagacg ggactcgaac ccgcggcctc ttccttggca
     3241 aggaagcgtt ctaccactga actactctcg catagacgaa tatagtagat tttagcatat
     3301 ttctatatta tatgtcgtat ttatatgcaa tgccggtgat gggcgctttg cgttgtaata
     3361 gaaggtaaaa tccttatata cttcaacagg atcgttaatg tttgtgtttt gcgagtttta
     3421 tttattttta caggattttc taaaattagc tcaattttcg aaggagctta tctttaattg
     3481 cttcagaata actggagtag tttatcttct tgcttttgct ggcctcttcg tcagctcttg
     3541 aaactttagc ccacaaaatg tttcctagct catttacaga gttttctaag agctctgaaa
     3601 tcgatacggt aaaaccggca accgacacag ttggagggaa tattgtatta aaaatgttta
     3661 tatcttctct tttcgcatta tcgaaaactt ccgggctaaa attgtacggg attcctgatg
     3721 cgtactctct tactttttcg cccattattg tagctgtttc gtaatctacc caaggaaggt
     3781 cgagctcctt ataataagcc tcgtcctcta tagaaattag gggatttgtt gtgatcacta
     3841 tgccttcatc tccgctcaac ctagaaacta tagcgtttct ggaacttgcc tttattctct
     3901 gtactgcgca aactgttgct atagttccgc accccacagg ctctctccct aaatagtgat
     3961 taatgtcttt ttgatgattt atgtcaaaaa aatatatagc aatctgcgga tggtttttta
     4021 aaagtgaaga aataatgtga attgccccta ctctgtttgg cagaccggtc atctcgtctg
     4081 ttaacagcgc cgctttatac attggcattt taggaaaata ttttgtatat tcaggttttt
     4141 tgtatttatt gggcatataa caaactatct atgctatttt ggagcgagag acgggattcg
     4201 aacccgcggc ctcttccttg ggaaggaagc attctaccac tgaactactc tcgcgctcgc
     4261 aactttttta ttgtatacca tggcttgaga tagtaaaaga gtctgactgc accaaaattt
     4321 aaatttggat tataagaaat atgtttagat accaagtctt tatggtgggc aagacaggac
     4381 ttgaacctgc acaggatttc tcccacaacg acctcaacgt tgcgcgtata ccattccgcc
     4441 acttgcccgt gtacgagcat tatacaataa aaagcccctt actcaaataa ttgtatatct
     4501 ttaaaactat gtgcccactt agtttgtttg taaatttggg tagatgtggg cgcgctggtg
     4561 ccggagatgg gactcgaacc cacactccaa taattggaac agccttctga agactgcgag
     4621 tctgccaatt cctccactcc ggcccacata tcagttattt caagcaataa cttacatagc
     4681 aaaaggtatc ttatactaat attttgctaa tttaaagccc agagacaggg tcttctatat
     4741 ctatcgaaga ctcaggcatg ccttgaatct gaaacttcaa gataatgctg tcatcaacat
     4801 taaatacgtt taataaagac ggggcctcaa ctccaaaatc cgacattagg actgtggcct
     4861 ctccactaac atcgaacgta ctatcattgg taacagcgtt aacatcaaaa ataacctctt
     4921 tcttaacaga gttgatttcc aacattacag gaattgaaaa tgaaaattga cctgattcat
     4981 agtcttcggg aagctgaaag ccattaactg atattagtac tgagctatct ttaaatattt
     5041 taagaatgtc agagtccctt tgcgcagatg gtgattttag catatctatt gacaggcttg
     5101 cattcgcata taacatccta tcatccgaag tcacccaaat taagacatcg gttagttctg
     5161 cagagccttc tatttgttct ccgggcttat ttaaaaacat tttgttagcc ctatatccgg
     5221 cagtcgcatc actaattaaa tatagaaccg catcatctgg cagggcgttg tttttattgt
     5281 agccgagctc cgaggtcgca ggtatgttta tctgtttcat tacatttgga tcgtatctat
     5341 cgtctttggt tgctttgtgc ttttctttat tggtcagaga atacacaaca aaaactaaag
     5401 agaggagtat tactagagat aagactataa ttgtgggctt attgtttttc atactacgta
     5461 ttatatcaaa gcttgttaca attgttgtat gcaaaaaaat atagttattg ttggggcagg
     5521 ctttgcgggt ttatataccg caaaatactt ggaaaaaaga ctaaagctgc ttaaaagtaa
     5581 aaagattgaa gcgagcattg cattaattga ccagaacaac tactttactt ttacccctct
     5641 tcttcatgaa gttgcatcgg gtggcctcga aagagaacta atacttcacc ccataagaga
     5701 ggcctttggg actggcgtat cttttattaa ttctaaagtt gaaagggttc atttaggtaa
     5761 gaatttcgta atgacctctg ataaaattca gctgccttat gattatctgg ttttagcaac
     5821 aggttcagtt tcaagcttaa aatatgatgt tcttaataca gaaatattta aactaaagac
     5881 tgtaaacgac gcatataata ttaaatcggc catacttaca gtttttgaac gaaaacaaaa
     5941 tagcctgctc aacccccccg ataaaagtcg ttattgttgg tgggggacct accggtgtag
     6001 aactcgttgc agagctggct gaatacatgc atggaacttt agctaggctt tataaacatg
     6061 taggtccggg acaaaaccaa attttattat taaattctgc ggggcatctt ctaggatctt
     6121 ttgacaagag actgggtgaa agaattgaaa agtatttggt tcagaaactt aatgtaaaag
     6181 taataaataa ctcaaaaatt ataagtattg aagattttgt aattaaatcg agtaatggtg
     6241 aaataagagg agctgacgta ataattgacg cctcgggggt gagagcaatg tacccagact
     6301 taattccggc aatagacacg gacgaaaaaa acaacataat aacagatgat tttctacgaa
     6361 ttccggaata tccaaatgtt tatgctttgg gggatgttgg acagttttac aaactcgcac
     6421 agacagctgt aaatcaagct gaatatgcgg caaaaagcat tgtcaactta atgttgaata
     6481 aacagcctaa acaatatacc ttcaaaccca aaggattctt actgtcatta gggaaaaact
     6541 atgctatagg ttctcttttt aacgtgctga tatttggaaa gccggtttgg tttttatggc
     6601 gtacgatata cttatttaaa gttttaaact ctagacacag aattaaaatt gcactaaact
     6661 ggacattgaa tttattttca gatagagaca caactaacat atgaacaagc agtttttaca
     6721 gaaactaacc gcctttcaga attcttttaa tgcgaatttc tttttagaaa tagaagatct
     6781 ggaaaagcac atgatggaaa atatgtcaca tagcctgcaa gaggtttatc gtcgttataa
     6841 taaattatcc actaacggag gaaaaagaat aaggcccttt ttagcagatg tttcatacag
     6901 tgcttttggt gggcaagaca caaagaacat cagaaagcta tgcattgccg tggaactaac
     6961 aaattcctac atactggctt tagatgacga ccttgacaga acacaactaa gaagaaattc
     7021 tcccacagca aacaaatcta tctatgagta tctactaaca cttcgtccta caacagaaat
     7081 gtcgtacgca catcatttaa cctcatcttt aatatctatt acgtctttaa tgtatatgca
     7141 ttacataaac tcttttatca tagagcaaac tacatttgaa gaaagtgtaa agctgcgcct
     7201 tatagataca ctagctaaaa tgatttttaa aacagcccag ggagaaaaca gcgaaacagt
     7261 attgatgcta gatagagaca tttcaattaa aagatcactt gatattttac ttactaaaac
     7321 cggctattat acaattgaga cgccagttag aatgggctat tttgctacag gagaagaaaa
     7381 ccccagtgta acaagctcct tagaagaata tgctcacaat gtcggtctgg cttttcaatt
     7441 aaacgacgac ataataggaa tctttggaga cacgaacatt gcaggaaaaa gtaattccga
     7501 cgacataaaa gaaggaaaaa taacaccttt ggttgcttac atttatgaaa atggctcatc
     7561 taaggacatc tccgtaatag aatctatatt gggtcgggca gatatcacag atgaggaggc
     7621 tgacaggttt agagacgtgg tcgtgtcttc gggaagtaaa gattttgtta gcaatttggc
     7681 cagagaatac gcagataaag gcaaagccag tattaaaaat gccgaaaaat ttgcagtgag
     7741 taaggatgca tttaatactt tctttgagtt agcagatttt gttatcgaca ggtcttttta
     7801 agcatgtggc cccatttgat atctggctat gttggaatca acgtaaaact acagccacta
     7861 tagtttaaac gattttcttc tgtagataag tagtaacaat gcaatcaata ttttttcttg
     7921 tgtcttagta taaactcgcc ctaaaaatgg attcatatct ctttttttta atttaaccag
     7981 gatttgaaaa tataggtgtg cactcaacaa tattgagtat ctagtctttt taggtagtag
     8041 gtcggctcct tttatccctt ctttgtaaaa gcacactgtt ttattagcat aatcaagcat
     8101 caggtcgtgc aattcgttcg aaaaagaccc tgaaaatatt tgttctcttg taacaccaaa
     8161 tgagcccatg tctgtagtag ataggtatat gcggcctcgt ctagcgtctt cctgtatgtc
     8221 cc
//
LOCUS       DAOSWN010000029         7978 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_5821_length_7978_cov_4.329294, whole genome shotgun sequence.
ACCESSION   DAOSWN010000029 DAOSWN010000000
VERSION     DAOSWN010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7978
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5821_length_7978_cov_4.329294"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..1514)
                     /locus_tag="PLF29_02100"
     CDS             complement(48..1514)
                     /locus_tag="PLF29_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52753.1"
                     /translation="MKNYNFYLKEYGEIGHVEKIVTPIVEVAGLPSCTISEMVVFEDG
                     SLGEVIAVDQEKATVLLYEHRNLDVGQRVARTGSGPTVKYSESLFGSMVDSFGDEIAL
                     STKSKSLESDYVDLQIYTSASDISTRSVVSEPFITGVTLIDLMTPLGRGQRELLIGDR
                     KTGKTSFALQLLLAQKDKESVNIYACIGKNKSEVQYVKDFLIKNDLLKKTIIVASFST
                     SAVGKIYSTPYAAMSIAEKLSKEGKNVVLVLDDMLSHAKYYREISLLANIFPGRDSYP
                     GDMFYVHARLLERAGNFNNGTSITCFPIVSTANDELSGYIETNLMSMTDGHLFFDRGR
                     FYEGLRPAVDPLLSVTRVGRQTQNSLRWAINRELNSFFVLHKKTGAFIHFGAEVSEGV
                     KATIGMGDRLNILFAQPAQESYTLELQMLMLVLVWSNYLSNISGNDALKKRLIEIKEF
                     YESDKNFQNIVLAAVAESKNFNELIRNIIGAVAKFGIK"
     gene            complement(1511..1969)
                     /locus_tag="PLF29_02105"
     CDS             complement(1511..1969)
                     /locus_tag="PLF29_02105"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HXK52754.1"
                     /translation="MKIPSIYTKETLDQIVFDLSSLRLSYNPNDEKGFKQKLLTKFSS
                     QSAAFFLEVLENVPFEDIPKILDEFEKFLGSLRVVTLVLAFAPRPEFIQEIHNRVSKI
                     VPEACVLDIVVDGAVVGGFSIDFSGKFFDFTLAQDLIKRDLSEELKSCSP"
     gene            complement(1966..2700)
                     /locus_tag="PLF29_02110"
     CDS             complement(1966..2700)
                     /locus_tag="PLF29_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52755.1"
                     /translation="MPIYTLIQNNNIWILFVFEIIILCLFFSIIYKAIKFLLVRAELF
                     GSSDLEKSLSYDKASKIIEYAQRRAIKILARASIDAEKKLRGATKVNKDLQEELFQNL
                     NRVYVSHIKEFEGLSESLLLNYKAVLQRESAVGAQVLEEMGASVEEMVKAEGKEFRKN
                     LESRTLLAEEKLSNEIDEEYKKVKKEIELYKESRLKEVEEKVNALLYSIISQYISKDI
                     SVTIHEELVKAHFDRLVQELQLKKKV"
     gene            complement(2705..3412)
                     /locus_tag="PLF29_02115"
     CDS             complement(2705..3412)
                     /locus_tag="PLF29_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52756.1"
                     /translation="MDNGVGISVEVPYSSIQGSFPDPGSIVSFRDGLYSLSSAEYDSN
                     VIGVIPSDSVITINDDSLSSSIFVMTSGETFIKVSGAGGNILEGDYITSSDVSGVGKK
                     AETSGQVVGIALESHDFASTEEVYDLYSYVTIRSVYFDDALKVNLMELLQNGFRVPYL
                     TPLTTLRYILASLIAVFSFIGGLRLFGKTSTNSIEALGRNPLASSVIRSTVVFNFILT
                     AAIILAGLSISYLILVL"
     gene            3700..>7978
                     /locus_tag="PLF29_02120"
     CDS             3700..>7978
                     /locus_tag="PLF29_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HXK52757.1"
                     /translation="MSAQNIKNTSNNSESNNLLSVSEAAKILGVSSSTVRRLAEQAII
                     IENRDANGYRRFNVNDIINYKNHRLNTTKNPIENLAYVPINTRPKENLSESSASKPTE
                     STAKTEAKNIVKKVKFKQGPLKRAVAKTQKNNVFKKAILASVVIGAALLAITIAKDFN
                     NLSRETPDKNIQSALSIKKYIANYKRGDEKGTQRIQRQEAEVLAARARSPELRFTLNV
                     PTNILSSLNITGPATVEGGVSTTTINFTGASTINGLLAIDAVSKNSLENSLELTGDII
                     GTLNANALADNIIDGNNLVPDFTYEDNFNLNGTLAIGGTWSIGGTNVNATAEELNFLE
                     GSTADEGGIIFGSGSGFMQDTAYLFYNSATHALGINTNNPQSPLSVGTASQFQVNASG
                     DIIKINNVTYAWPTTQAGAASYVLANDGNGTLAWTTVGAASVGPDSLDFTEFKDAMTL
                     DDSTDIAITGANQFSLTNTGTGVSFLVNDSAGDTSPFVIDSSGNVGIGKIAPAQALDL
                     VGAMVLENTSSPTTGVIYKESASFIHNYHWEGNTGQNTFVGENSGNFTLGQGSPDASI
                     ASYNTGLGYNALSTLTYGRSNTAVGAGALSNIDSGNYNSALGAAALYSNTNGNYNSAF
                     GSSALYSNTTGNDNLAFGPFAMFNNTTGVSNVALGKHALASNTTGSYNLVLGNYALYF
                     NTTGGNNIAAGYNALYFNSTGNRNIAVGGSALYSNTSGANNIATGDHALYSNTTGSYN
                     FAAGFYALYFNSTGNYNIATGDYALASNTSGPANIATGSYALYSNTTGNSNVATGYNA
                     LYFNSTGSFNTAFGNSALYSNSTGNYNIAAGAFALYSNTSGSNNIAAGYLALFSNTTG
                     TGNISLGNDSLSSNTTGSYNYAFGPSALRRNTTGGSNVAVGNNSMYYNTIGTGNIAIG
                     GTTLYTNTTGNYNIASGISALYFNTTGSNNIATGTNALFRNTVGTDNIATGRDALIFN
                     TSGSFNVAVGSSALYSNTTASYNSAFGYSALYSNTTGNSNVAIGRSALYSNTTGSYNS
                     ALGTSTLYSNSTGDYNLAAGYFALYENTSGSYNTAVGPYTLRQNTTGSYNSAFGTNAL
                     RTNTTGNYNVGVGYTALRYNTTGSDNAALGYQALFSNVSGNGNSAVGSGALYTNTGGN
                     YNSSFGSYALYSNTTGSQNSAVGYFSLTTNTTGIDNTAVGYFSLQANTSGSQNSSFGA
                     YALSSNTTGNYNIAFGSEALYLNTYGYYNVALGHQALRANSTGGQNIATGYRTLYSNL
                     TGQSNAGVGFETLYTNSTGSSNSAFGEQALYENTTGSYNTTSGVASLYHNTTGYWNTA
                     IGVFAGTYLADGASPNSTSITSLYLGSQTKALADGGENEVVIGFGAIGAGSNSVVLGN
                     DSILTTLLKGVVGIGTTSPSAELHVLGSFKLEDSYGKSTSLYTESFGS"
BASE COUNT         2601 a         1737 c         1434 g         2206 t
ORIGIN      
        1 tatccagtta attggggtta ggtacatata ttaattcttt tttttcatta ttttattcca
       61 aacttggcaa ctgcaccaat tatattcctt attaattcat taaagttttt cgattcagca
      121 actgctgcca aaactatatt ttgaaaattt ttatcgctct cataaaactc ttttatttca
      181 ataagtctct ttttaagagc atcgtttccg gaaatattgc ttaaataatt actccaaaca
      241 agaacaagca ttagcatttg tagttctagc gtgtatgatt cctgggcggg ctgtgcaaac
      301 aaaatattca aacggtcccc cattccaata gtagctttta caccttcgct aacttcggct
      361 ccaaaatgaa taaaggcccc tgtttttttg tgtaacacaa aaaaactgtt taattctcta
      421 tttatagccc acctcaaaga attttgagtc tgtctcccaa ctcgtgttac tgacaataga
      481 ggatccacag ccggacgcaa accttcgtaa aacctgcccc tatcaaaaaa gagatgtccg
      541 tcagtcattg acataaggtt tgtctcaata taaccggaaa gttcatcgtt tgcagtagaa
      601 actattggaa agcacgtaat cgatgtgccg ttgttaaaat tacctgccct ttctaaaagc
      661 ctggcgtgaa cgtaaaacat atctcccgga taagaatctc tacccggaaa aatattagcc
      721 agcaagctta tctctctgta gtatttagca tggcttaaca tgtcatccag caccagaacg
      781 acgttttttc cttctttgga gagtttttct gcaatactca ttgcagcgta aggagtagaa
      841 tatattttac ctactgctga agtagaaaaa gaggcgacaa ttattgtttt tttgagcaga
      901 tcgtttttaa tcaaaaaatc ttttacgtac tgaacctctg acttattttt tcctatacaa
      961 gcatatatat taaccgactc tttgtctttt tgcgcaagaa gtagttgcaa tgcaaaagag
     1021 gttttacctg tttttctatc tccaattagt aactcccttt gacctcgtcc cagaggcgtc
     1081 atcaaatcta taagcgtaac cccagtaata aatggctcag aaacaacact tcgggtggat
     1141 atatcagaag ctgaagtgta aatttgcaaa tcaacataat cgctttctaa acttttgctt
     1201 tttgtgctta atgcaatttc atctccaaac gagtcaacca tgctaccaaa aaggctttct
     1261 gaatatttta cggtcggccc ggagccggtc ctagcgactc tctgacctac gtccaaatta
     1321 cgatgctcat ataaaagtac ggttgccttt tcttgatcta ccgcaataac ctctcctaaa
     1381 gagccgtctt caaaaacaac catctcactt atagtacaag aaggcaaacc tgcaacttca
     1441 acaataggtg taacaatctt ttccacatga cctatttcgc catattcttt tagataaaaa
     1501 ttataatttt tcatgggcta caagacttga gctcttccga tagatctctc ttgataagat
     1561 cttgagcaag ggtaaaatcg aaaaacttgc ccgaaaaatc tatggaaaaa cctcctacca
     1621 cggcaccatc aacaactata tccaatacac aagcctccgg cacaatttta gaaacacggt
     1681 tatgaatctc ctgtataaac tcgggacgcg gggcaaatgc taaaaccaaa gttactacac
     1741 gaagagaacc aaggaacttt tcaaattcat ccaatatttt cggaatatcc tcgaagggaa
     1801 cattttccaa aacttccaaa aaaaacgcag ctgattggct tgaaaactta gttaacaact
     1861 tctgcttaaa ccctttttca tcatttgggt tgtaagaaag tcttaaagaa gagagatcaa
     1921 aaactatttg atctaatgtt tcttttgtat aaatagacgg gattttcata cttttttctt
     1981 tagttgtaac tcttgaacta aacgatcaaa atgagctttt actaactcct catgaatggt
     2041 tacagatata tctttactaa tatactgact aattatggaa tataggagag cattaacttt
     2101 ctcttccacc tcctttagac gactttcctt atataattcg atttcttttt taactttttt
     2161 atattcttcg tcaatttcat tggataactt ctcctcggcc aacaaagttc ggctttccag
     2221 attttttcta aattctttac cttcggcttt aaccatctcc tcaacactag cacccatctc
     2281 ctctaatacc tgggctccaa ccgcagattc cctctgcagt acggctttgt aattcaacag
     2341 caaagattca gacagccctt caaactcttt tatatgactt acataaaccc tatttaaatt
     2401 ctggaaaagc tcttcctgga gatctttatt aactttcgta gctccgcgca atttcttttc
     2461 ggcgtcaata ctagccctgg ctaaaatttt tatagcccgt cgctgagcgt attcaataat
     2521 tttagaggct ttatcgtaac ttaaactttt ctctaaatca gacgaaccaa acagctcggc
     2581 cctgactaat aaaaatttta tagccttgta tattattgaa aagaataaac aaagaataat
     2641 aatttcaaaa acaaatagga tccagatatt attattttgt atcaaagtat aaatgggcat
     2701 actcttataa tactaaaatt aaataagaaa tcgataaacc tgctaatatg attgctgcgg
     2761 tcagtataaa gttaaaaact acggtagacc taataacgct acttgccaaa ggatttctcc
     2821 ctaaagcctc aatgctgtta gttgatgttt taccaaacag cctcaaacca cctataaaag
     2881 aaaaaacggc tattaaagag gcaagaatat atcttaaggt tgttaaggga gttaaatacg
     2941 gaactctaaa accattttgt aaaagctcca ttaaatttac ttttagggca tcatcgaaat
     3001 acacagagcg gatagttaca taagaatata aatcgtacac ctcttccgta gatgcgaaat
     3061 catgactttc taaggcgata ccaaccacct gcccgcttgt ctctgccttc ttgcccacac
     3121 ccgaaacatc cgaagatgtt atataatctc cctctaaaat atttccaccc gcaccagata
     3181 cttttatgaa agtttcaccg cttgtcatga caaaaatgct gcttgataaa gaatcatcgt
     3241 taatcgtaat aacagaatcg gaaggtatta cacctatgac attactatca tattcagcgc
     3301 ttgacaacga atacaaaccg tctcgaaagc taacgatcga tcccggatcg ggaaaagatc
     3361 cctgaatact gctgtaagga acttccactg aaatacctac cccattatcc aaaactgttt
     3421 gcgagtatat gacactgtat gtagaaataa aatagataag agatactatg aaatactgta
     3481 atataatacg aaataagcta cttttcgaat tggatattaa acttgaaaaa aaatcaaagt
     3541 ttccgggcag aacctgcata taaaaagcat acgcaaacag gcatcacaag tcaataactt
     3601 gacctttttg acagatataa tcatttgaca tcgaactatt atttacttat tattagattg
     3661 gttatcaaat tgctacatag cggtatttga taaagtttta tgtctgccca aaatattaaa
     3721 aacacatcaa acaattctga atcaaacaat ctcttatctg tttcggaggc tgctaaaata
     3781 ctgggggtct cctcctctac cgtaagaagg ttagcagaac aagcaataat catagagaac
     3841 agagatgcta acggatacag gcgttttaat gtcaatgaca taataaatta taaaaatcac
     3901 agacttaata cgacaaaaaa cccgatcgaa aaccttgcat atgttcccat aaatacaagg
     3961 cccaaagaaa atcttagcga aagcagtgct agcaaaccaa cagaatcaac tgctaaaaca
     4021 gaagctaaaa atattgtgaa aaaggttaaa tttaaacagg gccccttaaa acgagcagtg
     4081 gcaaaaacac aaaaaaacaa tgtatttaaa aaagctattt tggcctcagt tgtaataggt
     4141 gctgctttgt tggcaataac aattgcaaaa gatttcaata atctaagccg cgaaactccc
     4201 gataaaaata tacaaagtgc actatcaata aaaaaatata tagcaaacta taagcgggga
     4261 gatgaaaaag gcactcaaag aatacaacga caagaggcag aggttttagc cgcaagagcc
     4321 agatctcctg aactcagatt tactctaaat gtaccaacaa acatattgag ctcattaaat
     4381 atcaccggcc cggcaacagt tgaagggggc gtctcaacta caaccataaa ctttaccgga
     4441 gcatcgacaa taaacggctt gctggcaata gatgcggtct ctaaaaactc tcttgaaaac
     4501 tcgttggaac ttacaggcga cattattgga actctaaatg cgaatgctct tgcagacaac
     4561 attatagacg gtaataatct ggtgccagac ttcacgtacg aagataattt taatttaaac
     4621 ggaactttgg ccataggagg cacctggagt ataggaggaa caaatgtaaa tgctaccgcc
     4681 gaagaactaa attttttaga aggatctact gcagatgaag gcggcataat ttttggttcg
     4741 ggaagcggtt tcatgcaaga cacagcgtat ctattttata acagcgcaac acatgccttg
     4801 ggaataaata caaataaccc acagtcccct ctttcggttg gcacagcatc tcaatttcaa
     4861 gttaatgcct caggggacat tattaaaata aataatgtta cttacgcatg gccaaccaca
     4921 caagccgggg ccgccagcta tgtacttgca aatgacggta atggtactct ggcctggaca
     4981 actgtagggg ctgcctctgt cggtcccgac agcttagact tcactgaatt taaagatgcg
     5041 atgaccctcg acgattcgac agatatagct attacaggag cgaatcagtt ctcacttaca
     5101 aatacaggaa caggggtatc gtttttagtg aacgactccg cgggagatac ttcacctttt
     5161 gtcatagact caagcggtaa tgtggggata ggaaaaattg cccctgcgca agcgctagat
     5221 ttagtaggag caatggtatt ggaaaataca agctccccta ctacaggagt catatataaa
     5281 gagtccgcaa gttttatcca caattatcac tgggaaggta atacaggaca aaacacattt
     5341 gtaggggaga atagcggaaa ttttactcta ggacagggtt ctccggatgc tagtattgcg
     5401 agctataaca ccgggctggg ctataacgca ctgagtactt taacgtatgg tcgctcaaat
     5461 acagcagtag gtgcaggggc cctgagcaat attgacagcg gaaactataa ctccgcccta
     5521 ggagccgccg ccctatattc aaacactaac ggaaactata actcagcctt tggaagctcg
     5581 gccctatatt caaacactac cggtaatgac aatttagcct ttggcccctt tgctatgttc
     5641 aacaacacca caggagtcag caacgtggct ttaggtaaac atgcactagc ttccaacacc
     5701 acagggagtt acaacctggt tctaggtaac tacgcattgt attttaacac tacgggagga
     5761 aacaatattg ctgcaggcta caacgcacta tactttaact ctacaggaaa tcggaacatt
     5821 gctgtaggag gaagtgctct atattcaaat acttcagggg ccaataatat tgccacagga
     5881 gatcacgctc tatactcaaa caccacagga agctataact ttgctgcggg attttatgct
     5941 ctatacttta actctacagg aaattataat attgctaccg gagactatgc cttggctagc
     6001 aatacttcag gtcctgctaa tattgctacc ggatcttatg cgctctactc gaacaccaca
     6061 ggaaactcta acgttgcaac gggttacaat gctttgtact ttaactctac aggaagtttc
     6121 aacactgcat tcggaaatag tgctctatac tccaactcta caggaaatta taatattgcc
     6181 gccggagctt tcgcgctata ttcaaacaca agcggcagca ataacattgc agccggttat
     6241 ctagctttat tctcaaacac tacagggaca ggtaatattt ctctaggaaa tgactcttta
     6301 tcttccaata caacgggaag ttataactat gcatttggtc ccagcgcgct ccgaagaaac
     6361 actacggggg gaagcaacgt tgctgtagga aacaattcta tgtattacaa cactattgga
     6421 accggcaaca ttgccatagg cggaactact ttatacacca atactacagg caactataac
     6481 attgcctcag gcatatccgc cctatatttt aacaccaccg ggagcaataa cattgcgaca
     6541 ggaacaaatg ctttgtttag aaatactgtc ggaaccgaca atattgccac cggtagagac
     6601 gcgctaatct ttaatacttc gggaagcttt aatgttgctg tagggagtag cgcactgtac
     6661 tcaaacacta cagcgagcta taactctgca tttggatatt ctgctttgta ttcaaacact
     6721 acaggaaact caaatgttgc aataggaaga agtgccttat actcaaacac taccggaagc
     6781 tataactcag ctttaggcac ttctaccttg tactcaaact ccacaggaga ctataaccta
     6841 gcagcgggat atttcgcttt atatgaaaac actagtggga gctacaacac cgcagtcggg
     6901 ccctatacgc taagacaaaa tacaacagga agctataact ctgcatttgg taccaatgcg
     6961 cttcgtacaa acactacagg gaactataat gttggggttg gttatacggc tttaaggtac
     7021 aatactaccg gcagcgacaa cgcagcctta ggttatcaag ctttattttc aaacgttagt
     7081 gggaacggaa attcagctgt aggttccggg gctttatata caaacacagg aggtaactac
     7141 aactcctcct ttggatctta cgctctatac tcaaacacta caggaagcca aaactcagcg
     7201 gttggatact tttcgttaac aacaaatacg acagggattg acaacacagc agttggttac
     7261 ttctctttgc aagcaaacac ttcgggcagt caaaactcgt cctttggagc ttacgcgctg
     7321 tcctcaaaca ctaccggaaa ctataatatt gcattcggat ctgaagcttt atatttaaac
     7381 acttacggat attataacgt tgccctcggc caccaggctc tccgagcaaa cagcacgggg
     7441 ggtcaaaaca tagctacagg ctaccgaact ctttactcta acttaaccgg acaaagtaat
     7501 gccggggtcg gctttgaaac tttatataca aactctacgg gatcaagcaa tagcgcattt
     7561 ggggaacaag ctctatacga aaatactaca ggtagttaca acacaacatc aggcgtagct
     7621 tctttgtatc acaacacaac agggtactgg aacaccgcta ttggggtctt tgcaggaaca
     7681 tatttggcag acggcgcaag tcctaattca actagcatta catccttgta tctcgggtcg
     7741 caaactaagg cattagcaga tggcggagaa aatgaggtag taataggatt tggggctatc
     7801 ggagccggaa gcaacagcgt tgtactagga aacgacagca ttcttactac actactaaag
     7861 ggggtcgttg gaatcggaac cactagccct agcgccgaat tgcatgttct tggaagtttt
     7921 aaactggaag acagctacgg caagagcacc tctctctata cagaaagctt cggtagcg
//
LOCUS       DAOSWN010000030         7791 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_6004_length_7791_cov_5.089452, whole genome shotgun sequence.
ACCESSION   DAOSWN010000030 DAOSWN010000000
VERSION     DAOSWN010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7791
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6004_length_7791_cov_5.089452"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="PLF29_02125"
     CDS             <1..864
                     /locus_tag="PLF29_02125"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HXK52758.1"
                     /translation="DGEAIGYDRETGEFLPFQQTIQRKRKHNISELAQEIPLKYFVFD
                     ILYLNGESLLNKTLAERKKILGGVIKSNDNTTDTIVVVNYQTFSEAKKLSAFFEKVRE
                     KKLEGIIAKNPMAPYQAGARSYTWVKFKVADDTLLDDTIDVVILGYYLGRGIRNEFGI
                     GGFLAGIFDSATQTYKSITKVGTGLKDEELKFLKSQADKIMVKGAPKEVEVPKGLAPD
                     VWTKPQLVVEISGDEITKSPSHSSGFAMRFPRLIGFRVDKNVRDITTLKEIEDLYKLT
                     TGRRPEKDARQ"
     gene            851..1918
                     /locus_tag="PLF29_02130"
     CDS             851..1918
                     /locus_tag="PLF29_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52759.1"
                     /translation="MPDNEAITISRTPNEEIITRREFIPRLGASLAATFFDLRKKRDG
                     LLHFPITRDEPTLEPIRTDIEILGNSYPKRDGPEDAHKDTLTGMLHDRVVEKTCSILP
                     PLYVPWASAILGFVPKAVVKEAVYEVGSFGDPILTAILTIGAATHEILYKAPSKTLYD
                     LTGANMLGRHGLEGVILPVLKRGDRSNGLNGGIKPPMFPPNWLPAIGITRGAVQGEIS
                     ILREELGPLATNIIEGLMPRNPSLGFPPAPETHQISTGKEIDVQDINILINLMWALLA
                     EPRMRRKAQDPKGPLQQGRLEEAVQILALYNVSTTIWALRAGLAYATRVKTNDDPLVT
                     ILSALAYSLEAISLENSAVRA"
     gene            1926..2249
                     /locus_tag="PLF29_02135"
     CDS             1926..2249
                     /locus_tag="PLF29_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52760.1"
                     /translation="MQTLADILKNKKANRDHRNSYEFQAFGNRLAEELGDIKHRALYI
                     KLAKTEDRNLLLQALDHIKASPNATTKGRLFMWKVKQLKAASAEKEQATEKKPHPKKN
                     EKETN"
     gene            2319..2864
                     /locus_tag="PLF29_02140"
     CDS             2319..2864
                     /locus_tag="PLF29_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52761.1"
                     /translation="MIRHIFNFYKIHFLISLTIAIIIITKSSVANPLAFVAVIASSIM
                     GGIIFEADYLAFAYFLDKESIFSQTLRGYLKDKDFKNALTYTQFHKNEVTDKPLNSAL
                     FQFALTVLGGLIAFSSSLLFVKALVFSALAVSIYKAIQIYFDNNLSSWFWILKEQPKR
                     NGFFIYIGTLTILLVYFLYLI"
     gene            2868..3527
                     /locus_tag="PLF29_02145"
     CDS             2868..3527
                     /locus_tag="PLF29_02145"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HXK52762.1"
                     /translation="MYGFFITGAILIYALLTERALGKKKLDVNIFWGGLFYAIVWGIV
                     GARLYHVLDFRSYYYSNPTQALEIWKGGMGIYGAILGTLLSTTAYLAIKKQPVRLWLD
                     ACAINAPLGQAIGRLGNVYNQELLPYAYFEIAGNIIIWLFILAINNLFDKKTTHYKSG
                     AIFLIYIFMYSTQRLVLESTRSVHWVEWGLNVGVAVSFLLAMVSLITIVFLYKKPGIK
                     I"
     gene            3540..3983
                     /locus_tag="PLF29_02150"
     CDS             3540..3983
                     /locus_tag="PLF29_02150"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HXK52763.1"
                     /translation="MLYITSDHGGYELKTKLINLFKKAGVKITDLGPSEFLEDDDYPK
                     YAKKLAEKMSQNTEYSRGIILCRNGVGVSMAMNRFEHIRCALSWSPKHAKSSRKDDNT
                     NVLALPADYVSYSEAKNIIGAWLNTSFSKQERHVRRLSELEDILK"
     gene            3980..5263
                     /locus_tag="PLF29_02155"
     CDS             3980..5263
                     /locus_tag="PLF29_02155"
                     /inference="COORDINATES: protein motif:HMM:NF013030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HXK52764.1"
                     /translation="MKIYPTILEQSIEKVLNNIEGLGGTAKHIQIDMCDGTLVEGKTF
                     VDPSPLFDLNFEQNLTLELDLMVAVPEKYIFQNGHISKIYVLSKAIKSKAHFKMLEQL
                     CAENRIDLGISFSLDTSDREMAVFSSYTNNVQFLTVVPGGQGRKFEPEAFKNALKFAK
                     KNPDHLLQLDGGINTKTLKEYFSYSVIRSINSVVAGSAIFAKNRPDEAYLELQKVIKN
                     IMSEKKVQQKKSSEKLVPIEYSGVIKSAGFFGGAALTEKDQAYKEAFAVAKLLAENGI
                     AVINGGGPGIMKAATKGTHAGGKEVLVVTYSPSYKHKNYEGTDPENDFDFEITTKDYF
                     DRTKIMLQNTDLHIVFIGGTGTLSELGMSWANSRIHEGHHKPIILYGNFWEEIIAALE
                     KYLLLREGEADLVKICTSPEEVLDFIKKYNNEHLN"
     gene            5291..6184
                     /locus_tag="PLF29_02160"
     CDS             5291..6184
                     /locus_tag="PLF29_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HXK52765.1"
                     /translation="MPQYSPANNKFQYCTDKEICTIEKKAVELRESVVEMLVEAGSGH
                     IAGALGTADIFAVLYHKILNIDPKDPHHENRDRFILSNGHICPVWYAVLAQVGFFHKN
                     KLKTLRKLDSGLQGHPRKNSVPGIENSSGSLGQGLSQAIGLALGAKLSELSYRVYCML
                     GDGEMQEGQVWEALMYAGNKRLNNLTCIIDRNNIQISGYTENVMPLESLREKLEAFNW
                     HVLEVDGHNVEELVNSFNMAKAVFERPTAIVAHTIPGKGADFMEYKFEWHGKVPSAKE
                     AKKALNALRSLEGRIKCYDDE"
     gene            6177..6713
                     /locus_tag="PLF29_02165"
     CDS             6177..6713
                     /locus_tag="PLF29_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52766.1"
                     /translation="MNKNNQTKLNINLLPLFLILVLILGAGYFLLGDEIKLPKLRKDP
                     TEVTRLEGYPTFIYNQEDMEKQRLVINSREQLNEFLNTIDSTGLLTIKEEIDFDKEMV
                     IAVASSTNPTLGYKIKIKKVIEDQEKGVYTVSIIETEPGESCNEEPEKNIAVDIVRIT
                     KTDKDIEFERAKETKECN"
     gene            6704..7708
                     /locus_tag="PLF29_02170"
     CDS             6704..7708
                     /locus_tag="PLF29_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HXK52767.1"
                     /translation="MQLKPALAVNTNLLLNDRMHGLNPSLKSTKKAFGESLLALGKED
                     ENVVVLCADLNSSTKTDDFAKNFPDRFFQVGIAEQNMAGIAAGLAACGKKVFITSFAA
                     FSPMRNLDQIRVSICFSDLPVIMVGAYAGLSPSADGATAQELEDIAIMRTLPNTVVLS
                     PVDYEQTKKATEASVTLSKPVYMRLCREDTPEITTASTPFDVKKAQTLIEGDDLTIVC
                     TGPICHEAIIATKKLTENGIKPEVINVHTIKPFDKTLLLQSATKTRLVITVEEHQQSG
                     GLGGAVAEILSEEMPTKVLRIGTRDTFGESGTYAQLKNKYGVDSEAIYSLAVNNINKK
                     "
BASE COUNT         2670 a         1575 c         1606 g         1940 t
ORIGIN      
        1 gatggtgaag cgatagggta tgacagagaa accggcgagt tcctaccctt tcaacaaaca
       61 attcaaagaa aaagaaagca caatataagt gaacttgctc aagaaatacc tttaaaatac
      121 tttgtgtttg acatcttata tctaaacggc gagtctttac ttaacaaaac tctagctgag
      181 cgcaaaaaaa ttttaggcgg tgtaataaaa agcaacgaca acacaacaga tacaattgta
      241 gttgtcaact accaaacatt ttctgaggcc aaaaaactaa gtgccttttt tgaaaaagta
      301 agggagaaga agcttgaggg cataattgca aaaaatccta tggcccctta tcaagcaggg
      361 gcaagatctt atacatgggt gaaatttaag gttgcagacg atacgctact ggacgacact
      421 atagatgtag ttattcttgg ctattacctc ggcagaggaa tacgcaacga gtttggcata
      481 ggaggttttt tagcaggaat cttcgatagc gcgacccaaa catataaatc aattacaaaa
      541 gttggtacag gtctaaaaga tgaagaatta aagtttttaa aatcccaagc agacaaaatt
      601 atggtgaaag gcgcgccgaa agaagttgaa gttccaaaag gcttagcgcc ggatgtctgg
      661 acaaaaccac agcttgtagt tgaaatatca ggagatgaaa taacaaaatc cccttctcac
      721 tcttcaggct tcgctatgag atttcccaga ctaataggtt ttagggtgga taagaatgtt
      781 agggacatca caacactaaa agaaatagaa gatttataca aactaacaac gggaaggagg
      841 ccggagaaag atgccagaca atgaagcaat aactatctct aggaccccaa atgaagaaat
      901 aattactagg agagagttta ttcccagact cggagcttcc ctcgctgcta ctttttttga
      961 tcttaggaaa aaaagggacg gtcttcttca ttttcctatc acaagggacg agcctacact
     1021 agaacctatc agaacagaca tcgagatcct tggcaatagt taccctaaaa gagatgggcc
     1081 ggaagatgct cataaggaca cgcttacagg aatgttgcac gatagagtag tggaaaaaac
     1141 ctgcagcata cttccacctc tctatgtgcc ctgggcgtca gctatacttg gatttgttcc
     1201 gaaagctgtt gttaaagaag cagtatatga agtgggaagt ttcggagacc ctatactaac
     1261 cgcaatttta actatagggg cggccaccca cgaaatactt tacaaagctc ccagtaaaac
     1321 cttgtatgac ctcacaggag ctaatatgct agggaggcac ggccttgaag gagtgatact
     1381 gcctgttctc aaaagggggg atcgcagcaa cgggttgaat ggggggatta aaccacccat
     1441 gtttccacct aactggctac ccgccatcgg catcactaga ggggctgtac agggtgaaat
     1501 ctcaatatta agagaggagt tagggcctct tgcaactaat ataatcgaag gtttaatgcc
     1561 cagaaatcct tctcttggat ttccacctgc gcccgagact caccaaataa gcacggggaa
     1621 agagatcgat gttcaagata ttaacatatt aataaatctc atgtgggcac tgctcgcaga
     1681 accgcggatg cgcaggaaag cacaagaccc gaaagggcct ttgcaacaag ggcggctgga
     1741 agaagctgtt caaatactag cactttacaa cgtcagcacc acaatatggg ctttacgtgc
     1801 aggcctggcc tacgcaacca gggtaaaaac aaacgatgat cccctagtga ctattctttc
     1861 ggcgctcgcc tattccctgg aggcgatatc tcttgaaaac agtgcagttc gtgcctagaa
     1921 atacaatgca aacactagca gacatattaa aaaataaaaa agccaaccga gatcacagaa
     1981 actcatatga atttcaggct ttcggcaaca ggcttgctga agagttggga gacataaaac
     2041 atcgggcgtt gtatataaaa ttagcaaaaa cagaagatcg caacctgcta ttacaagctc
     2101 tggatcacat aaaggcctcg cccaacgcta caacaaaagg ccgcctattt atgtggaagg
     2161 tgaaacagtt aaaagcagcc tcggccgaga aagagcaagc cacagaaaaa aagccgcatc
     2221 caaagaagaa tgaaaaagaa acaaactaaa gccgcgcaag ttctcttttt tgttaagttc
     2281 gttgcaaata ccacgcacaa ctactaaaat aaaaattaat gataaggcac atttttaatt
     2341 tctataaaat acattttttg atctctctca caatagcaat aataataatt acaaaatcct
     2401 ctgtcgcaaa tcccctagcc tttgtggcag ttatagcctc aagcataatg ggcggaataa
     2461 tttttgaagc ggactacctg gcctttgcat actttttaga taaagagtca attttctctc
     2521 aaaccttacg gggctatctc aaagataaag atttcaaaaa cgccttaacc tacactcaat
     2581 ttcacaaaaa cgaggttacc gataaacctt taaacagtgc ccttttccag ttcgcgctta
     2641 ctgtgttagg cggccttatt gcattttcat cctcattgct ttttgttaaa gctctagtct
     2701 tttcggccct tgccgtaagt atttataagg ctatacagat ttacttcgac aacaaccttt
     2761 ccagctggtt ctggatactc aaggagcaac caaaaagaaa tgggtttttt atttatattg
     2821 gaactcttac tattttgcta gtttatttct tatacctaat ctaaatcatg tacggttttt
     2881 tcataaccgg tgccatttta atatacgctc tacttacgga gcgtgcactt ggtaagaaaa
     2941 aacttgatgt aaacatattt tggggaggtt tattttatgc tattgtgtgg ggaattgtag
     3001 gcgcaagact ttaccatgta cttgatttcc gatcgtatta ctactctaac ccgacccagg
     3061 ctttggagat ttggaaagga ggaatgggca tttatggcgc gatattaggt actcttttga
     3121 gcacgacagc gtacttagca attaaaaaac agcccgttcg tctttggctt gacgcatgcg
     3181 ccataaatgc ccccttagga caagccatag gcaggctcgg aaatgtttat aatcaagaac
     3241 ttctaccata tgcctatttt gaaattgcgg gaaatatcat aatttggcta tttattttag
     3301 caataaacaa tttatttgat aaaaaaacaa cacactataa aagtggggcc atttttttaa
     3361 tatacatttt tatgtactca acgcaaaggc tggtattaga gagtacaagg agtgttcatt
     3421 gggtagaatg gggccttaat gtaggcgtag ctgtaagctt tttacttgca atggtttcac
     3481 ttattaccat tgttttttta tataaaaaac ccggaattaa aatatagtat acttaaacaa
     3541 tgctctatat aacctctgac catggcggtt acgaacttaa aacaaaacta ataaatcttt
     3601 ttaaaaaagc cggtgttaaa attactgact tagggccgtc tgagttttta gaagacgatg
     3661 actatccaaa atatgcaaaa aagcttgccg aaaaaatgag tcaaaacacc gagtactctc
     3721 gaggcatcat tttgtgcaga aacggggtcg gagtcagcat ggccatgaac agattcgagc
     3781 atattcgatg cgccttatcg tggtcaccaa aacacgctaa aagctccaga aaagatgaca
     3841 atacaaatgt cttagctttg cctgccgact acgtatccta cagcgaagca aaaaacataa
     3901 taggggcatg gctgaacact tctttttcaa aacaagagcg ccacgttaga aggctttcag
     3961 aactggagga tatactaaaa tgaaaatcta cccaactatc ttagaacagt cgatagaaaa
     4021 agttctgaat aacattgaag ggcttggggg cactgctaag catattcaga tcgatatgtg
     4081 cgacgggact ttagtagaag gtaaaacttt tgtagacccg agtcccctct ttgatttaaa
     4141 ttttgaacaa aacttaactt tagaactaga tttaatggtg gccgttcctg aaaaatatat
     4201 ttttcagaac ggccacatct caaaaatata cgttttgtca aaagcaataa aaagtaaggc
     4261 ccacttcaaa atgctcgagc aactatgtgc agaaaataga atcgatctcg gaatctcatt
     4321 ttccctagac acatcagata gagaaatggc tgtcttttct tcctatacaa acaacgtaca
     4381 gtttttaacc gtagtgccgg gaggacaagg cagaaaattt gagccggaag cttttaaaaa
     4441 tgctctgaaa tttgctaaaa aaaacccaga ccacctactt caactagacg ggggtataaa
     4501 cacaaaaact ttaaaggagt actttagtta ctctgttatc agatcgataa actcggtcgt
     4561 tgccggttcc gcaatatttg caaaaaatag acccgatgag gcatacttag aattacaaaa
     4621 agttataaaa aacataatga gtgaaaaaaa agttcaacag aaaaaaagct ccgaaaaact
     4681 agtacctata gaatattccg gagtaataaa atctgcgggg ttctttggcg gagcagcact
     4741 aactgagaaa gatcaagcct ataaagaggc ttttgccgtg gctaaacttc tagcagaaaa
     4801 cggcattgcg gttataaacg ggggcggacc cggtattatg aaggcggcca ccaaaggaac
     4861 acacgcaggg ggcaaagaag tactggtggt tacctacagc ccaagctaca agcataaaaa
     4921 ctatgaaggc accgatcccg aaaacgactt tgatttcgaa attacaacga aggattattt
     4981 cgatcgtaca aaaataatgt tgcaaaacac agacctgcac atcgtattca ttggagggac
     5041 aggaacactg agcgaactcg gcatgagttg ggccaactct cgaatacacg aggggcacca
     5101 taaaccaata atcttatatg gaaatttctg ggaagaaata attgccgctc ttgaaaaata
     5161 ccttcttttg cgcgaaggtg aggccgacct tgtgaaaatt tgcacgagcc ctgaagaagt
     5221 tcttgatttt ataaaaaaat ataacaacga acacttgaat taaaccgtaa taaattagat
     5281 actatactta atgccccaat actcgcctgc aaacaacaag tttcaatact gtaccgataa
     5341 agaaatatgc acaattgaaa aaaaagccgt ggagcttagg gaatcagttg tagaaatgct
     5401 agtagaggca ggttcgggac acatagccgg agctcttgga actgcagaca ttttcgcagt
     5461 gctgtaccat aaaatactta acattgatcc taaagaccct caccatgaaa acagagacag
     5521 gttcattctt tcaaacgggc acatatgccc tgtctggtat gcggttttag ctcaagtagg
     5581 attttttcac aaaaacaaac taaaaacact aaggaagctt gattcgggtc ttcaaggcca
     5641 tccgcgcaaa aactctgtac cgggcattga aaactcgagc ggttctctag ggcaaggact
     5701 atcccaagct ataggacttg cccttggcgc gaaactctca gaactatcat acagagtgta
     5761 ctgcatgcta ggagatggcg aaatgcagga agggcaagtc tgggaagccc tgatgtacgc
     5821 cggaaataag aggttgaata accttacgtg tataatagat agaaacaata ttcaaatcag
     5881 cggttacacg gagaatgtta tgccgctaga atctttgagg gaaaagttgg aagccttcaa
     5941 ttggcacgtc ctagaggtag acggacataa cgtagaggaa ctagtaaact cctttaatat
     6001 ggctaaggca gtctttgaaa ggccgaccgc tattgttgcg catacaatcc ccggcaaagg
     6061 ggccgatttt atggagtata aatttgaatg gcacggaaaa gtacctagcg caaaagaggc
     6121 aaaaaaagct cttaacgccc ttagatcctt ggaaggccgc ataaagtgtt acgacgatga
     6181 ataaaaacaa tcaaacaaaa ttaaatataa acttacttcc cctgttctta attctagtat
     6241 taatcttagg tgccggatat tttcttttag gggatgaaat aaaattacct aaacttcgaa
     6301 aagacccaac tgaagttact aggttggaag gctacccgac atttatatac aaccaagaag
     6361 acatggaaaa acagcgactt gttataaact ccagagaaca attgaacgag tttctcaata
     6421 ctatagactc aacgggactg ctaacgatta aagaagagat cgacttcgat aaagaaatgg
     6481 ttattgctgt agcaagttca acgaacccca ctttaggcta taaaataaaa attaaaaaag
     6541 ttatcgaaga tcaagaaaaa ggcgtatata cagtttcaat aattgaaacc gagcccggcg
     6601 aaagctgcaa cgaagagccc gaaaaaaaca ttgcggttga tattgttaga ataacaaaaa
     6661 ccgataaaga tattgagttc gaaagagcca aggaaacaaa ggaatgcaac taaagcctgc
     6721 ccttgctgta aacacaaact tgcttctaaa cgatcggatg cacggactta acccgagcct
     6781 aaaatccacg aagaaggctt tcggagaaag ccttcttgca ttaggaaaag aagacgaaaa
     6841 tgttgtggtg ctatgtgcgg accttaatag ttctacgaaa accgacgact tcgcgaaaaa
     6901 ttttccagac agattttttc aggtcggaat agcagaacag aacatggcag gtattgcggc
     6961 cggacttgcg gcatgcggaa agaaagtttt tataacctcc tttgccgcct tttcgcccat
     7021 gagaaacttg gatcaaatta gagtttcaat ttgcttctct gatcttcctg taataatggt
     7081 aggtgcttat gcggggctgt ccccctccgc cgacggagct acagcccaag aacttgagga
     7141 catagctatt atgagaaccc ttcctaatac cgtagtgctt agtcctgtag actatgaaca
     7201 aacaaaaaaa gctacagaag ccagcgtgac cttgagcaag ccagtgtaca tgagactttg
     7261 cagagaagat actcctgaaa taacaacagc ctcaactcct tttgacgtaa aaaaggcaca
     7321 aacccttatt gaaggcgacg atctgacaat agtatgcacc ggaccaatat gccacgaagc
     7381 aataattgct acaaaaaaac ttaccgaaaa cggcataaaa cccgaagtta taaatgtgca
     7441 cacaataaaa ccttttgaca aaactctgct tctgcaatct gcaacaaaaa caagactagt
     7501 cataactgta gaagaacacc aacaatcagg cggccttggc ggtgcggtgg ctgaaattct
     7561 tagcgaggaa atgcccacga aagtcttacg cataggaaca agggacacat ttggagaatc
     7621 aggaacttac gcacagctga aaaataaata cggagttgat agtgaagcta tatacagcct
     7681 ggcagtaaat aatataaata aaaaataaaa tactacatac gtaagtctgt attctcttct
     7741 ctactgtcgc aaacccgcac cttcatctgc gaaatgaggc tagcagtctt a
//
LOCUS       DAOSWN010000031         7737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_6063_length_7737_cov_5.164020, whole genome shotgun sequence.
ACCESSION   DAOSWN010000031 DAOSWN010000000
VERSION     DAOSWN010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7737
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6063_length_7737_cov_5.164020"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..1609)
                     /locus_tag="PLF29_02175"
     CDS             complement(26..1609)
                     /locus_tag="PLF29_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52768.1"
                     /translation="MMLYKMASKIKSPGKPKTQNNEIGPNLKKNLFLVLVWILILGTA
                     AYLRFYNINFGLPQSFHADEPELVEPAIKYTYEIRKIISNRSYEQLIPISFVYGQVPT
                     YILTPIIMTSSKLMNTLGYAFSKETLYVIARALSALLSLVIIPLTLALFNLVNKRESA
                     NKIKAALVVVLATAYNWKLIVIGHYANADIYLTFFTLLSSIFFLQYIQSSTKKFLVLS
                     AVFFGLSVGTKVTALIVLPIYFFLLFKNKRIFAIAIFTLLTFGAFIFSNPFSWALAQD
                     FAFRVLTLATKENGLVFDSANLSYTKYLVALAQQASPIVLAAAVLATAQTSLKLFRKK
                     ENDTDRATKDAHIYLILYAMFYIVFYSISNRLVDRWLLPVLPIVFVYAGVFIEKLNGK
                     LGNIIFALFVLLLIYSYGEKAFLLTKQYQRQTPKSEAYIWAQSNISGPPTKLVVTEEG
                     LDPMNKLLSTTVLTFNVYESENAYLEFPPNPSLYNYVILSSRPMSYFKNPIKARNTLH
                     MWLLGTVSSPQYRIKKTLS"
     gene            complement(1617..2231)
                     /locus_tag="PLF29_02180"
     CDS             complement(1617..2231)
                     /locus_tag="PLF29_02180"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HXK52769.1"
                     /translation="MQIIDISIEIFENMPTYPGNPNVSFTPVIKRNSQITTISFGTHT
                     GTHIDAPLHTIPSGSPIDAIDLEAFVGPCTVVDFSQKNILSISGPMLEKISLTKNNKV
                     LFKTDNSYSEGEVFTKDYVYIEPSAAEYLAQQKVSLVGIDALSIKKSGDTDNTPHTAL
                     LSKNIPILEGINLKAVSEGEYFLVCAPLKLKGLDGAPARAFLIK"
     gene            complement(2218..3477)
                     /locus_tag="PLF29_02185"
     CDS             complement(2218..3477)
                     /locus_tag="PLF29_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52770.1"
                     /translation="MEFIYKRYAAIDETVLLEQSTLTNEYTEYLRHVAEKNSYEYPES
                     SINLPFDEDIFNLMIPLKEKMLSKPVKRIVLVGIGGDNLGTKAVYEALKTEKTTEILF
                     LDTLDEPQLEKILKILEDDGSDDTLIIIASKSGKTIETLYNSSMLLQVNKAYIHRTIF
                     ITSENSPLWNFAQKNNCPALPIPLTVGGRYSVFSAVGLFPLFCAGIDTVKLLEGAMEA
                     RNNCLDKNILNNPARISAQLIFGNLEKDKNILDMLVFAPKLEALAKWYRQLVAESLGK
                     DGKNITPTISVGTTDLHSVWQSYIGGHRNKQLCLVTLKHANKTYKIKGAVELPSEVKL
                     KNINLIQLRNSIQAGVKETLLENRIPFTEIALGLIEEKEIGNFMQFKMMEVMQLGKLL
                     KINAFDQPDVEGYKRKSLENLKIYADH"
     gene            complement(3477..4913)
                     /gene="zwf"
                     /locus_tag="PLF29_02190"
     CDS             complement(3477..4913)
                     /gene="zwf"
                     /locus_tag="PLF29_02190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HXK52771.1"
                     /translation="MPEIKAPTNLILFGFTGDLVQKKVIPSLFHLYKEGRLPELFNVV
                     GFSRRTLTVDEVQEIIFTSLENHPDLGKNARPQDMIAFAKKFQYISGDFVDQSSYTAM
                     GNILGRVDESWSTCSNKLFHLAVPPKYYKEIFLQLKKSNLTKPCGADEGWTRVIVEKP
                     FGRDLKTAKELDMLLGKLFKEEQIYRIDHYLGKEMMENILMFRFANNLLEPVWNSKHI
                     EKIEVEVLEKTDIGSRGVFYDEVGALRDIGQNHLLQMLALVLMENPYKNGPRESLRTK
                     RAKLLKELSKTKVNPNKNSYRGQYTGYTKTEGVDKQSFTETYFLLKTQVSNQTWQEMP
                     IYIECGKSMEQTNKKIKVTFKPLNSFGHHNRLTIDLDGDDAGIYIDLWAKKPGLGLVP
                     EKRTLSFSLCDNRDECTNNSEEYESLIYDCLEGNQMRFLTTDELQYMWKVVDPVLKSW
                     QKQKHNGLKLYKKGTEQIRQDARARVGI"
     gene            4972..5340
                     /locus_tag="PLF29_02195"
     CDS             4972..5340
                     /locus_tag="PLF29_02195"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HXK52772.1"
                     /translation="MNTKKIAKIIDIGTMVVGFLLGFRIILKFLGASTAAPFVKWLYE
                     FTSGMLAPFNGIFPSPMLGRTSVVDISAFFAMIVYLLLGYFIGELVESSGGTLKIRDW
                     RKKNYKDITKDDTLSQNPRV"
     gene            complement(5473..7284)
                     /locus_tag="PLF29_02200"
     CDS             complement(5473..7284)
                     /locus_tag="PLF29_02200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXK52773.1"
                     /translation="MSKNNLKQNLLATLLVIFPIMLYELNNNLLQGLLVIGVSVYLGC
                     FYYILRKQFIFSDRALKIIVLAACASAIFLSITHPRKNSYDGHNAYEKMVVNLVNTNF
                     NIEYLGIPQYPFLGELSLAVLYKALGREYYLIFFALTGIGFVFSAHLLFRELAIKKEI
                     ARFAVFLLVCNSVFAAMLLHEYKIDHFLGMLSNIFFILFIKVLKTGSLKYYLLAGVIG
                     GAALLTKVTFGPILALCLAFGTIYKIKLQAKERPILIKKAVLELAKFSLTAIVVVFLV
                     LLWTYKFGLYVGPNIYFYPQTQKIKPLALSTNKEVAAACNKEILAKDQGLFLPKYKFP
                     LMQPIKVIFWSSLGEGRASNAISIARLETTMYLGVFSLSLWPLYMFVKKKPLGIAELL
                     IIISSVYVWLFFLEVQTLIWYLIPVFSIIALIASSTTTALIKDGIIKTYLVFLILGTT
                     TVSVIFLLSISFNGLKVSGTGEKTKQDPYITSGYDLVVNTSSTIDSVIGNNPGSYILD
                     AALHPYFVFTTYTSNYYATITRNNLYFLENTDTDLMRDELISKNVRYILVSRANLLNT
                     WYDGCAEENNRTLDKFLNKYTVPIFPLTRTPVLFKIL"
     gene            complement(7271..7609)
                     /locus_tag="PLF29_02205"
     CDS             complement(7271..7609)
                     /locus_tag="PLF29_02205"
                     /inference="COORDINATES: protein motif:HMM:NF021575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HXK52774.1"
                     /translation="MTLYQIILLTALGIFFLKQLVSIKKTSGKNFFRTYVWLILTFAG
                     ATVIADPRLTAVLAQKFGIGRGTDIVVYTVIGWLFYKMYRADQQISKQQEQLNKLVSR
                     MALKDTNEQK"
BASE COUNT         2199 a         1410 c         1528 g         2600 t
ORIGIN      
        1 ggtttgggca aggagaaaga cctaatcaac tcaaagtttt ctttattctg tattgtggac
       61 tcgaaactgt accaagcagc cacatatgta gggtatttct cgctttaata gggtttttaa
      121 agtaagacat tggtcgtgaa ctaagtatta cataattata taggcttggg ttaggaggaa
      181 attctaggta ggcattttcg ctttcatata cgttgaaggt taaaactgtt gttgagagca
      241 gtttattcat tggatccaga ccttcttctg tcaccacgag ttttgttggt gggccgctta
      301 tgttggactg cgcccaaatg taagcctcgg acttaggagt ttgtctttga tactgttttg
      361 ttaatagaaa agctttttct ccatatgagt agattagcaa taaaacaaat aacgcgaata
      421 tgatattccc caacttcccg tttagttttt caataaacac gccggcatat acaaaaacta
      481 ttggaagtac cggtaacagc catcgatcga caagcctgtt gcttattgaa taaaatacaa
      541 tataaaacat agcgtacaaa atcaaataaa tatgcgcgtc ttttgttgct ctgtcggtat
      601 cattttcttt ttttcgaaaa agttttagtg aagtttgcgc cgtagcaagt actgctgcag
      661 ctagcacgat aggagatgct tgttgagcta gcgcaactag gtattttgta tatgataaat
      721 ttgccgagtc gaaaacaagg ccattctctt ttgttgctag ggttagaact ctaaatgcaa
      781 aatcctgtgc taaagcccat gaaaacggat ttgaaaaaat aaaagcccca aaagttaata
      841 gtgtgaatat cgcaatcgca aagattcttt tatttttaaa tagcaagaaa aagtatattg
      901 gcagaactat gagtgcggta acttttgtcc ctacagaaag cccgaaaaaa acagccgata
      961 agacaagaaa tttctttgta ctgctctgta tgtattgcaa gaaaaagatt gatgagagca
     1021 aggtgaaaaa agtcagatag atatcggcat ttgcataatg tccaattaca attaatttcc
     1081 agttatacgc tgttgctaaa acaacaacca aagccgcctt tattttgttt gcactttccc
     1141 ttttgtttac aagattaaat aacgcaagag ttagagggat tattactagc gaaagcagtg
     1201 cagaaagtgc tctcgctatt acatacaatg tttcctttga gaaagcgtaa ccaagcgtgt
     1261 tcattaattt gcttgaagtc attattatgg gtgtcagtat atatgtgggg acttggccat
     1321 aaacaaaaga aataggtata agctgctcat acgacctgtt tgatattatt ttacgtattt
     1381 cataagtgta ttttattgcc ggctcaacaa gttcaggttc gtctgcatga aagctttgcg
     1441 gcaatccaaa atttatatta tagaatctta aataagcggc tgttcccaag attagtatcc
     1501 ataccaatac taaaaataag ttcttcttca gattcggccc gatttcgtta ttttgagttt
     1561 ttggttttcc tggtgatttt attttggagg ccattttata taacatcatt gtagagttat
     1621 tttatcaaaa acgcccttgc cggagctccg tccaggcctt ttaactttag cggtgcgcat
     1681 actagaaaat actccccttc agagaccgct ttcagattaa tgccttcaag aatgggtata
     1741 ttttttgata agagcgctgt gtgtggtgtg ttatctgtgt cgcccgactt ttttatagat
     1801 aaagcatcaa ttcctactag tgatactttt tgctgggcta gatattcggc ggctgaaggt
     1861 tctatatata catagtcttt tgtaaaaacc tccccttcac tataagagtt gtctgtttta
     1921 aaaagcactt tattgttttt agttaaactt attttttcta gcattggccc agagatagac
     1981 agaatatttt tttgtgaaaa gtctactaca gtgcacggcc ctacaaaagc ctcgaggtct
     2041 atagcgtcga taggagagcc tgaaggaata gtgtgtagcg gggcatcgat gtgggttccg
     2101 gtgtgtgtgc caaaagatat cgtggttatt tgtgagtttc ttttgatgac tggtgtaaaa
     2161 ctgacattgg ggttgccggg gtatgttggc atattttcga agatttctat tgatatatca
     2221 atgatctgca tatattttaa ggttctctaa actttttctt ttgtaaccct ctacatcggg
     2281 ctgatcaaag gcatttattt taagaagttt gccaagctgc atgacctcca tcattttaaa
     2341 ctgcatgaaa ttacctattt ctttttcttc aattaaacct aaggctattt ccgtaaaggg
     2401 aattctattt tccagaagtg tttcctttac acctgcttgg atgctgtttc taagctgtat
     2461 tagatttata ttttttaact ttacctctga cggtaactct acggcacctt ttattttata
     2521 ggttttgtta gcgtgcttca gggttactaa acagagctgt ttgttcctgt gcccgcctat
     2581 atatgattgc catacagagt gcaggtcagt agtgcctaca gaaatagttg gggtaatatt
     2641 ttttccgtct ttgcctagac tttcagcgac aagctgacga taccattttg caagagcctc
     2701 tagtttgggt gcaaaaacta acatgtcgag aatattttta tctttttcga ggttgccgaa
     2761 aataagttgt gctgagattc gagcgggatt gtttaatatg tttttatcca agcagttgtt
     2821 gcgggcctcc attgcacctt caagaagttt aaccgtatca attcccgcgc aaaaaagagg
     2881 aaacagccct acggctgaaa atacagaata tctgcccccg accgttagtg gtattgggag
     2941 cgcggggcag ttattttttt gcgcaaagtt ccataaagga ctgttttctg aagtgatgaa
     3001 tattgttcta tgtatataag ctttatttac ctggagaagc atagaagagt tatacaaggt
     3061 ttcaattgtc ttgcctgact tgcttgcaat aataattagg gtgtcgtcag atccatcgtc
     3121 ttcaaggatt ttcaatattt tttccagctg tggttcgtcc agtgtatcta agaataaaat
     3181 ctcggttgtt ttttctgttt ttaaagcttc gtaaacagct tttgtgccaa gattgtctcc
     3241 ccctatgccc accaaaacta ttcttttaac aggtttagag agcattttct cttttagagg
     3301 gatcattaag ttgaatatat cttcgtcgaa aggcaagtta atagagcttt cagggtattc
     3361 atagctattt ttttctgcga cgtgtcttag gtactctgtg tattcgttag ttagggtcga
     3421 ttgttccagc aatactgttt cgtcaatagc agcatatctt ttgtatataa actccattag
     3481 attcctacac gagctcgtgc atcttgcctt atctgctcag ttcctttctt gtaaagtttt
     3541 aaaccgttat gtttttgttt ttgccaagat ttcaaaacag gatcaacaac tttccacata
     3601 tactgcagtt cgtcggttgt taaaaacctc atctgattcc cttccagaca atcgtagatc
     3661 aacgattcgt attcttcgga attgtttgtg cactcatcac gattgtcaca cagtgaaaag
     3721 ctgagagttc tcttttcagg cacgaggcca aggcccggct tctttgccca caagtcaatg
     3781 taaatgcccg catcgtctcc gtcaagatca atagtgagtc tgttatggtg gccaaaagag
     3841 ttcaaaggtt taaaagttac ttttattttt ttatttgttt gttccatgct ctttccgcac
     3901 tcgatgtata tgggcatttc ctgccaggtt tgatttgaaa cctgtgtttt taaaaggaag
     3961 taagtctcag taaaagattg tttatcgacc ccttcagttt tagtgtagcc cgtgtattgc
     4021 ccgcgataag aatttttatt tggatttact tttgttttgc tcaattcctt aagtagtttt
     4081 gcacgcttag ttcttaggct ttctcttggc ccatttttat aagggttttc cattaaaact
     4141 agggctagca tctgcagaag gtggttttgg cctatgtctc tcagtgcacc gacttcatca
     4201 taaaaaactc ctctcgaacc tatgtccgtt ttttccaata cctcgacttc aattttttca
     4261 atgtgtttag agttccaaac cggctcgagc aggttgtttg caaatctaaa catcaaaata
     4321 ttttccatca tctccttgcc tagatagtgg tcaattctat aaatctgttc ctctttgaaa
     4381 agtttcccca aaagcatgtc aagctccttg gctgttttaa gatctcttcc gaatggtttt
     4441 tcaacaatta ctctggtcca cccctcatct gccccgcagg gcttagttag atttgacttt
     4501 tttagttgta aaaatatctc cttatagtat tttggaggca cggccagatg aaaaagttta
     4561 ttggagcagg tgctccagct ctcgtctacg cgacctagta tatttcccat tgctgtgtaa
     4621 ctgctttggt cgacaaagtc cccgctgata tattgaaatt tcttggcaaa ggcaatcatg
     4681 tcttgtgggc gtgcgttttt ccctaaatcg ggatgattct ccagtgatgt aaatataatt
     4741 tcttgtacct catcaacagt tagcgttcgt cgtgaaaagc caactacatt gaatagttcg
     4801 ggcagtctgc cctccttata aagatgaaaa agagacggta tgactttttt ttgaaccaaa
     4861 tctccggtaa atccgaataa aattaagttt gttggggctt tgatttcggg cattataaaa
     4921 attataacac caatgctttt catgcaccta aagatgtgcg aatattaatg tatgaacact
     4981 aaaaagatcg caaaaataat tgatattgga actatggtag tcggattttt gttaggtttt
     5041 agaataattc taaaattttt aggagcaagc actgcggcac cgtttgttaa atggctgtac
     5101 gagtttacct ctggcatgct tgccccgttt aatggcatat ttccgtctcc aatgttaggg
     5161 aggacttcgg ttgttgatat ttccgctttc tttgccatga tcgtgtatct tttactgggg
     5221 tatttcattg gggaattagt tgaaagttcg ggaggaacac ttaaaattcg agattggcgt
     5281 aaaaagaatt ataaagatat aactaaagac gacactctct ctcaaaatcc tcgggtttag
     5341 gaaattttac cgtcttatta accgccacca gcttacgcag gtggtttgct gtaatttctt
     5401 tgctctgcaa agcccagaaa tgttaggtaa aatacatctg ctgatttacg caagtagttt
     5461 taagtaacga aattataaaa ttttgaacag caccggagtc cttgttaatg gaaagatggg
     5521 caccgtatat ttatttagaa atttgtccaa agtcctgttg ttttcttccg cacatccgtc
     5581 ataccatgtg tttagaagat ttgctctgga aacaagtatg tacctgacat tttttgaaat
     5641 gagctcgtcg cgcattagat ccgtgtctgt attttcgaga aagtatagat tgtttctcgt
     5701 tatagttgcg tagtagtttg aagtgtaggt tgtaaagaca aaatatggat gtaaggcggc
     5761 atcgagaata taagatcctg gattgttgcc tattacagaa tctatagtag aagaagtgtt
     5821 tacaacgaga tcatatcctg acgtgatgta tggatcttgc tttgtttttt cccctgttcc
     5881 agagaccttt aatccgttaa atgatatgct cagtaaaaat ataacagata ctgttgtggt
     5941 tccaagaata agaaaaacta ggtatgtttt tattatcccg tcttttatta acgctgtggt
     6001 tgtagaggag gcgatcaggg caattataga aaaaacagga attagatacc aaattagtgt
     6061 ttgtacttct aagaaaaaaa gccacacata aacactgctt attataatca gtagctcggc
     6121 aatgcccaaa ggcttttttt tgacaaacat ataaagtggc cataaagaca gagaaaatac
     6181 tcctaagtac atagttgttt caaggcgagc aatgctaata gcgttgctgg ctctaccttc
     6241 tcccaacgaa ctccagaaaa tgaccttaat tggttgcatt agaggaaact tgtatttagg
     6301 taaaaatagc ccctgatctt ttgctaatat ttctttatta catgctgctg caacttcttt
     6361 gtttgtactt aaggccagag gtttgatttt ttgcgtttgt gggtaaaagt atatattagg
     6421 acctacatat agcccaaatt tatatgtcca tagtagtact aaaaaaacta ccactattgc
     6481 tgtcaatgaa aatttggcaa gttccaagac agcttttttt atcagtatag gccgctcttt
     6541 tgcctgcagc tttattttgt atattgtgcc gaaggctaag catagggcta gaatcgggcc
     6601 aaaagtaact ttagtcaaaa gagcggcccc tcctataact ccagccaaga gatagtattt
     6661 taaagaacct gttttgagta ctttgataaa caatataaaa aatatgttgg acagcatgcc
     6721 gagaaaatga tcaattttgt attcgtgcag tagcatggcc gcaaaaaccg aattgcacac
     6781 aagcaaaaaa actgcaaagc gagcaatctc tttttttatt gccagttcgc ggaaaagaag
     6841 atgcgcagaa aaaacaaacc ctatacctgt taatgcgaaa aatattaaat agtattcgcg
     6901 gccgagagct ttgtatagta cggctagtga taattcgcct aagaaggggt actgcgggat
     6961 tcctaaatat tctatattga agtttgtgtt caccaaattt accaccattt tttcatatgc
     7021 gttgtgtcca tcgtaagagt ttttcctagg atgtgttatg gaaagaaaaa ttgcagatgc
     7081 gcatgcagct aatacaataa tttttaatgc tctgtcggag aatataaact gctttcttag
     7141 aatgtagtag aaacatccta aatatacaga cacccctatt acaagcagtc cttgtaaaag
     7201 gttgttgttt agctcgtaca gcatgatcgg gaatattacg agtaaagtgg ctagtaggtt
     7261 ttgttttaga ttatttttgc tcattcgtat cttttagggc catgcggcta actagcttgt
     7321 tcaattgctc ttgttgcttg gagatttgct gatcggccct atacattttg tagaaaagcc
     7381 aaccgatcac agtatataca acaatatcag tacctctacc gatgccgaac ttttgtgcaa
     7441 gcaccgcggt cagtcgaggg tcggcaatta cagtcgctcc ggcgaacgta agtatcagcc
     7501 acacataagt tctaaaaaag tttttacccg aagtcttttt aatagatact agttgtttta
     7561 aaaaaaatat accaagcgcc gttaataata ttatttgata tagagtcatg tagatatttt
     7621 gagcaaatta attaccattg aaagagcatt tataaccctt tgtttaaccg gtttgttaag
     7681 tgagtattct gagtacaggg cttcaatgtc aacccagcct attttgcact ttttttt
//
LOCUS       DAOSWN010000032         7404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_6481_length_7404_cov_4.828956, whole genome shotgun sequence.
ACCESSION   DAOSWN010000032 DAOSWN010000000
VERSION     DAOSWN010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7404
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6481_length_7404_cov_4.828956"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..486)
                     /locus_tag="PLF29_02210"
     CDS             complement(142..486)
                     /locus_tag="PLF29_02210"
                     /inference="COORDINATES: protein motif:HMM:NF016045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HXK52775.1"
                     /translation="MPKTQSKTRKAFEGLSTRVSKIVGSPYWFGISLLMVVIWLPSGL
                     IIGFNAIWHLLINTATTILTFLMMSLLHAAQSDWEKKIEIIEARQEKLLKIMEKETEE
                     VIQTLQTDKPSK"
     gene            676..951
                     /gene="rpmB"
                     /locus_tag="PLF29_02215"
     CDS             676..951
                     /gene="rpmB"
                     /locus_tag="PLF29_02215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HXK52776.1"
                     /translation="MSVICEICGKGYLKGNLISTGIGKRVSRRTNRTQKPNIRNKRVE
                     INGQKVLVKMCASCLKRIKYEEKKLLAQNVADSAGSAETVAVSDTQA"
     gene            complement(984..1997)
                     /locus_tag="PLF29_02220"
     CDS             complement(984..1997)
                     /locus_tag="PLF29_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HXK52777.1"
                     /translation="MTNETNSLNVAFFGTSDKSEPILKALYSEFNLCLCVTKSDTKVG
                     RNRDIRQTGVKKWALGKNVHYIEVDNLKDRKNIDTVKKSLLMNNVDIAIVADFSYIIP
                     LEIIQEAKYGMINVHFSLLPKYRGASPVQQSLLNGDEYTGVTFILMDEGMDTGDILTQ
                     RSFPIEKLDTTASLYDKLFIFAADLLPQTIQDYVKNIISPKEQDHASATYCYSPTHPK
                     STYIYRDDARINWSKNVNIINNMIRAYFPWPVCWSTLQDMRQDSITLTSEGIILNNDK
                     NTTNAFDTFDLNVKNNKVIKIYEAEINNSNVLVIKRIQVEGKNITDWKSFLSGYSSKR
                     IGG"
     gene            complement(1994..2509)
                     /gene="def"
                     /locus_tag="PLF29_02225"
     CDS             complement(1994..2509)
                     /gene="def"
                     /locus_tag="PLF29_02225"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HXK52778.1"
                     /translation="MAIKEIVKVNKEKTPEVLLKQCAAVSNFDTETESLAQDLIDTLN
                     AKNHPEGAGLAAPQIGVLKRICLVKKYISESQELEIIMINPDITSKSSTKKVDLEACL
                     SIPNVYGYVQRSTNITVSFYDIKGRKHKIKASGFLARVIQHEIDHLDGILFTTKTIGK
                     VYTEEEVDHLI"
     gene            2818..5025
                     /gene="topA"
                     /locus_tag="PLF29_02230"
     CDS             2818..5025
                     /gene="topA"
                     /locus_tag="PLF29_02230"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HXK52779.1"
                     /translation="MSKLVIVESPTKAKTLSKILDKSYVIEASMGHIRDLPKKGLAVD
                     VEHNFEPSYEVPEKAQKVVNALKKKLADTDVVVLATDPDREGEAIAWHLQQILTSVKK
                     YKTMRYERVVFHELTRDAVLEAFTHPSELNLNLVDAQQARRVLDRLVGYKLSPLLWKK
                     VRFGLSAGRVQSVAVRLIVEKERERLAFIPEEYWTLISNLAFGVGEKKHSIKAELEKK
                     DDKKITIDNQKDAQKAQNDLEKALYTLSAVDKIERKRQANPPFRTSTLQQAMSNLYGF
                     TASRTMSAAQKLFEQGLITYHRTDSFNLAPQFVASARAYVKDNYGQEYLPTDPVFYKT
                     KSKNAQEAHEAIRPTDVFLHPSKLKKTIAEDARKVYVTVWRRALECQMAPAIYDQTTF
                     KITTNNNYGLKTTGSIIKFSGWMAIGEKLGLKMSGDDVALPDIKEGSALDLVSVESTQ
                     HFTQPPARYSDATLIKKMEELGIGRPSTYAPTLSTIQRRGYVEKEGKYYKPKPVALVV
                     TDLLVGNFPNIAGYDFTAEMENELDLIAEGEKKWVPVVKAFYEPFEKQVEEKDKTLSK
                     HDITNLGETDEKCPECGKTLIIKLGKFGTFLSCSGYPECEYARPIEGSSSENENGETV
                     DYGKCTVCEDGIFVLKQGRFGKFLACSNYPKCKTTKPFLEKIGMHCPDCSDGEVIVKK
                     AKGRSFYGCSNYPECKYASWKNPLQKDAKDRSTQDSSLVEIESDVAVDESEES"
     gene            complement(5045..6373)
                     /gene="glyA"
                     /locus_tag="PLF29_02235"
     CDS             complement(5045..6373)
                     /gene="glyA"
                     /locus_tag="PLF29_02235"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HXK52780.1"
                     /translation="MDKNVNRIIKMETRRQRAELQLIPSENYVSKNVLRALGSVFTNK
                     YAEGYPEKRYYQGNKYADQIEKLAIQRAKILFDTPFANVQANSGSPANLAVLFALLDV
                     GDTIMGMSLSSGGHLTHGHPKVTLSGKFFNSIQYGVSPEGFIDYKVMREMAHQHKPKL
                     IISGGSAYSRTIDFKLIGEIAKEVGAYHMADISHIAGLVVAKTHPSPTPFADIITTTT
                     HKTLRGPRGALILTTDAGTNKDPELASKIDKAIIPGTQGGPHLNNIAGIAVALGEAQS
                     PQFTEYINQVVLNSKALCNVFIKKGYTIISGGTDTHLLLLDVSAKNIDGWAAAWALEY
                     AGVIVNRNIIPFDTKTPFYPSGIRLGSPALTSRGMKEEQMEYVATIIDKTLNYTVRSM
                     DTNFSNNSKGENKEKRAVFLKKLKTDTYLKEIKKEVADFASRFPIYPTIE"
     gene            6516..>7404
                     /locus_tag="PLF29_02240"
     CDS             6516..>7404
                     /locus_tag="PLF29_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52781.1"
                     /translation="MESGRIIGRAGNERRAPSAVVNERPTEDAVIALENEEESYKIRA
                     AFLTLLIGAVDLKGRTKGPDTPSIKLSRIFHKVVALHRFGFTREAFELIISAAITNSL
                     SCAQDNKEAIVTSALVNFDAEELGLPPWQAKFYLSVCFDLLEQDCRVLEEGNTAIFGS
                     SERQALAFALNNNLTPDFINSALEEIFFPRLRQEGSSILPPADSLGKAYFSASLGLYR
                     IFLHHYRAIKNSSGGSAPVELESGTIVPAGNPPPFTGDSMRAMFNRVSARLLGIVTWA
                     EQGPLSLPRGTTPPLIGEER"
BASE COUNT         2115 a         1361 c         1560 g         2368 t
ORIGIN      
        1 actacttgtg taagctagta gatgtatttt atccaacatg ttgggctttg caacgtaaag
       61 aaattatagt aaaccacttg tgtaggccgg tggtggttga tttcggactt tttctattta
      121 tgttgattta ttggcaggat ttcattttga gggtttatca gtttgcaaag tttgtataac
      181 ttcttctgtt tctttttcca taattttaag aagtttttct tggcgagctt ctataatctc
      241 aattttcttt tcccagtcgc tttgggcggc atgcagtaaa gacatcatca aaaaggttag
      301 aatggttgtg gccgtattta tgagaaggtg ccaaatagca ttaaatccta taatgagccc
      361 agagggaagc cagatgacaa ccattaaaag cgaaatcccg aaccaatacg gactgcctac
      421 gatttttgat actcttgttg atagcccttc aaatgcttta cgtgttttac tttgtgtttt
      481 tggcattaca taaatgttaa tgttatttta acgccaagtc aaggtaaaaa tacgtctgtg
      541 acaattgcgt ttatagaaat tcaacctttg tacaagcatt tttagttaaa taaggcttta
      601 ggcttgtagt ttctactacc agcgctcctt cagctcattg acagcagcag tgctgtgtgt
      661 tttaatctca aagctatgtc agttatatgt gaaatttgcg gaaaaggcta tttgaagggg
      721 aacctcatat ctacaggtat aggtaagcgc gtgtctcgaa gaacaaatag aactcaaaaa
      781 ccaaacatta gaaataaaag agttgaaata aatggtcaaa aggttcttgt aaaaatgtgc
      841 gcttcttgtt taaagagaat aaagtacgaa gagaaaaagc ttttagctca aaatgttgca
      901 gattccgcag gtagcgcaga gactgtagcc gtttccgata cccaggcata gaaatttccg
      961 atcttaaatt atccttgtaa gttttagccc ccaattcttt ttgaagaata tccagataga
     1021 aagcttttcc agtctgttat atttttccct tcgacctgta tcctctttat cactaataca
     1081 ttagagttat ttatttccgc ttcgtatatt tttataacct tattgttttt tacatttaga
     1141 tcaaaagtgt caaacgcatt tgttgtattt ttatcattgt ttaggattat tccctcagat
     1201 gtaagagtaa tgctgtcctg cctcatgtct tgcaaggtac tccagcaaac tggccatgga
     1261 aaataagcac gtatcatatt atttataata ttgacattct ttgaccaatt aattctcgca
     1321 tcatctctgt atatgtatgt gctttttggg tgtgtagggg agtagcagta ggttgcactt
     1381 gcgtgatctt gttcttttgg agaaattata tttttaacat aatcttggat tgtttgcggg
     1441 agcaagtcgg cagcaaaaat aaatagtttg tcatacagtg acgcagtagt atcaagtttt
     1501 tctattggga aggagcgttg cgtaagtatg tcccctgtgt ccattccctc gtccatgaga
     1561 ataaatgtaa cccctgtata ctcgtcgccg tttagcaatg attgttgcac cggactcgcg
     1621 cccctatatt ttggtagtaa tgaaaaatgt acatttatca tgccatattt tgcctcttgt
     1681 ataatctcta gcggtattat gtagctgaaa tccgcaacaa tggctatatc tacgttattc
     1741 attaatagag acttttttac agtgtcaatg tttttcctat cttttaaatt atctacctca
     1801 atgtaatgca catttttacc cagagcccat ttttttacac cggtttgcct aatatctcta
     1861 tttcttccta cctttgtatc tgactttgtc acacataaac atagattaaa ctcagaataa
     1921 agggctttta aaatgggttc tgatttatct gacgtgccaa aaaaagcgac attaagtgaa
     1981 ttagtctcat tcgtcatatt aaatgatcta cctcctcctc ggtgtatact tttccgatag
     2041 tttttgttgt aaatagtatt ccatccagat ggtcaatttc atgctgaatt actcttgcca
     2101 gaaagccgga agccttaatc ttgtgcttcc gaccttttat atcatagaaa gacacagtaa
     2161 tgtttgttga acgttgtaca tagccataga cgttagggat gctaagacac gcctcaagat
     2221 cgactttttt tgtagagctt tttgaagtaa tgtcagggtt tatcattatt atttcaagct
     2281 cttgtgactc ggagatatac tttttaacta agcaaattct tttcagcacg ccaatttgtg
     2341 gggcggcaag tcctgcccct tcagggtgat tttttgcgtt taaagtgtca attaagtcct
     2401 gagcaagcga ctcggtctcg gtgtcaaagt tgcttaccgc ggcgcactgt tttaagagca
     2461 cttcgggtgt tttttccttg ttaactttga ctatttcctt aattgccatg ggtctatttt
     2521 atcatactga aatagattct gtgcttatac tgctgcaagg ccaacgtaaa taccgccttt
     2581 atttaagaac attcttcaat gtaatagaag atttttgact tttgctcatc tgattgagtt
     2641 taaagcattt tttaaacgct tttttgacaa ttattatgtt tcatgtattc ttttttaaat
     2701 agagcgagag atttttctct aggtatctgt tgaattagta atttttgtct gagaatctaa
     2761 taaatctcag cgttgttata aagtggtgct tgaaaggcca tttatataaa ggtagaaatg
     2821 tctaaacttg tcatagtaga atcgcctaca aaagcaaaga ctttatctaa gattttagat
     2881 aaatcttatg tgatagaagc ctcaatgggt catatcaggg atttgcctaa aaaaggttta
     2941 gcagttgatg tagagcataa ttttgaacct agttatgagg ttccggaaaa agctcaaaaa
     3001 gttgttaatg ctttaaaaaa gaagttagca gacacggatg tggtagtgct ggcaacagac
     3061 cctgataggg aaggggaggc aatagcctgg catttacagc agattctgac ttcagttaaa
     3121 aaatacaaga ctatgagata cgaaagggtg gtttttcacg agcttacacg agatgccgta
     3181 ttagaagctt ttacacaccc aagcgagctt aacctaaatt tagttgatgc acagcaggca
     3241 agaagagttt tagataggct ggttggctat aagctgtcgc ctttattgtg gaagaaagta
     3301 cgttttgggc tttcggccgg tagggttcag agcgtggctg taagattaat tgttgaaaaa
     3361 gagagagagc gtttagcttt tattcctgag gaatattgga ctttaatttc taatttagct
     3421 ttcggggtag gagaaaaaaa gcattctata aaggcggagc ttgagaaaaa agacgataaa
     3481 aaaattacaa ttgataatca aaaagatgct caaaaagcac agaatgacct tgaaaaagca
     3541 ctttataccc tttctgcagt tgataaaata gaacgaaaaa gacaagcaaa cccgccattt
     3601 agaacttcga cgctgcagca ggcaatgtca aatttatacg gtttcacggc atccagaaca
     3661 atgagtgccg cgcaaaaact ttttgaacaa ggtctgataa cgtaccacag aacagattct
     3721 tttaatttag ctcctcaatt tgtggccagt gctagggcgt atgttaaaga taattatgga
     3781 caagagtatt tgccgactga tcccgttttt tacaaaacaa agtcaaaaaa tgcccaagag
     3841 gcacacgaag ccattaggcc tacagatgta tttttacacc cttccaagct taaaaaaact
     3901 attgctgaag atgcacgaaa agtttatgta acagtttggc gcagggcgct agagtgccag
     3961 atggcgccgg ctatttatga tcaaacaact tttaaaataa caaccaataa caattatgga
     4021 ttaaaaacaa ccggctcaat aatcaagttt tccggctgga tggcgattgg ggagaagttg
     4081 ggtttgaaga tgtcgggaga tgatgtagct ctgcccgata ttaaagaggg ctcggcttta
     4141 gatttagttt ctgttgaatc aactcagcac tttacgcagc ctccggcacg ctattctgat
     4201 gccactttaa ttaaaaaaat ggaagaactg ggtatcggca ggccttccac ctacgcacca
     4261 acactaagta cgatacaaag acgcggatac gttgaaaaag agggtaaata ctacaaacca
     4321 aagcccgttg cattagtagt tactgattta ttggttggta atttccctaa tattgccggt
     4381 tatgatttta cggctgaaat ggaaaacgag ctggatttaa tagctgaggg tgaaaaaaaa
     4441 tgggtcccgg ttgttaaagc cttttacgaa ccttttgaaa aacaggttga agaaaaagat
     4501 aagacattaa gtaagcacga cattacaaat ttgggagaaa ctgatgaaaa atgccctgaa
     4561 tgcggaaaga ctttgataat taaacttgga aaatttggca cttttttaag ttgttccggt
     4621 tatcccgaat gcgagtatgc caggccaatt gaaggctcgt cttcggaaaa cgagaacgga
     4681 gagactgttg attatggtaa gtgcacagtt tgcgaagatg gtatttttgt attaaaacaa
     4741 gggcgttttg gcaaattttt agcttgttcg aattatccaa aatgtaaaac aacaaagcct
     4801 ttcttggaaa aaattggcat gcattgcccc gattgtagcg atggggaggt tatcgtgaaa
     4861 aaagccaagg gccgttcttt ttatggatgc agtaattacc cagagtgcaa gtacgccagt
     4921 tggaaaaacc ctttgcaaaa ggatgcgaaa gatcgaagca cgcaggattc ttctttagtt
     4981 gagattgagt ctgatgttgc tgttgatgag tcagaagaaa gctaactgag gcctaataag
     5041 gcttttactc tattgttgga tagatcggaa accttgatgc aaaatctgca acttctttct
     5101 taatctcttt taagtaggta tctgttttca attttttcaa aaacacggct ctcttttctt
     5161 tattttcccc ttttgaatta ttgctgaagt tggtatccat ggatctaacg gtatagttaa
     5221 gtgttttgtc tattattgta gccacatatt ccatttgctc ctctttcatg cccctggatg
     5281 ttagcgccgg tgagcctagc cttattccgg aagggtagaa tggggtcttt gtatcaaaag
     5341 gaattatatt gcggtttacg attacgcctg catactctaa ggcccaagct gcggcccacc
     5401 catctatatt ctttgcagat acatctagca ataatagatg ggtatcggtt ccgccggaaa
     5461 ttatcgtgta tccttttttt ataaacacat tgcagagtgc tttagagttc aggactacct
     5521 gattaatgta ttcggtgaat tgagggcttt gggcttcgcc caaagccaca gcaatacccg
     5581 caatgttgtt tagatgtggg cccccttggg tgccggggat aattgcctta tcaattttac
     5641 ttgctagctc agggtctttg ttagtaccgg cgtcagtagt taaaattaag gcacctcggg
     5701 ggccccttaa tgttttatgt gtcgtggtgg ttattatgtc ggcgaaagga gttggggaag
     5761 ggtgtgtttt tgcaactaca aggcctgcaa tatgtgagat gtccgccata tgatacgccc
     5821 cgacctcttt tgctatttct cctattaatt taaaatctat tgttctagaa tatgcgctgc
     5881 ctccggatat aataagtttg ggcttatgtt ggtgcgccat ctctctcatc actttgtagt
     5941 caataaaacc ttcaggactc accccatact gaattgagtt aaaaaacttt cctgatagtg
     6001 ttaccttggg gtggccgtga gttaagtgcc ctcctgacga caagctcatg cccattatcg
     6061 tatctccaac atctaaaagt gcaaataata ctgcaaggtt cgcaggggag ccggaatttg
     6121 cttggacatt ggcaaagggt gtatcaaata aaattttcgc tctctgtata gccagttttt
     6181 ctatttgatc ggcatactta ttcccttggt aatatctttt ttcggggtat ccttcggcat
     6241 atttattggt aaaaactgat cccaaagccc taagtacgtt ttttgatacg taattttcgc
     6301 ttggaatgag ctgaagttct gctcgttgtc tacgagtttc catttttata atccgattta
     6361 catttttatc cattgtttag cgattatata atgatttcaa atttatttac agattatcac
     6421 ggttattgtt gacacctgcc agaattgctt ctcacagacc tccttttatg ctataagtta
     6481 catatgtata cagaaaatgc cgaaggccat tttatatgga aagcggtcgc attataggac
     6541 gcgctgggaa tgagagaaga gccccaagtg cggtcgttaa tgagcgaccc actgaggatg
     6601 cggtaatagc gctagaaaat gaagaagagt cttataaaat tcgagcagcg tttctaactc
     6661 tattaatagg tgcggtggat ttaaaaggcc gaacaaaggg acccgatacc ccgagcatta
     6721 agcttagtcg gatatttcat aaagttgtag ctttacaccg ctttggtttt actcgagaag
     6781 cattcgagct cattataagt gccgctataa caaacagcct ttcttgcgct caggataata
     6841 aggaggcgat agtgactagt gctctagtaa actttgatgc cgaggaatta gggcttcctc
     6901 cttggcaggc aaaattttat ttgagtgtct gttttgatct tttggaacaa gattgccgtg
     6961 ttctcgaaga aggaaatact gcaattttcg gcagtagtga gcgacaagca ctggcctttg
     7021 ccttaaataa taacttaacc ccagatttta taaattctgc gctagaagaa attttttttc
     7081 cacgccttag gcaagaaggt agcagtatcc tgccccctgc tgattctctg ggaaaggcct
     7141 acttcagtgc aagcctgggc ctgtaccgta tttttttaca tcattatcgc gctataaaaa
     7201 atagctcagg tggtagcgcc cctgttgagt tggagtcggg tacaatagtg cccgcgggta
     7261 atccgcctcc ctttactgga gattctatga gggctatgtt taatagggtg tctgcgcgcc
     7321 ttttgggtat agttacttgg gccgaacaag gccccctttc actcccaaga ggtacaacac
     7381 cccctctgat cggcgaagag aggg
//
LOCUS       DAOSWN010000033         7386 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_6500_length_7386_cov_4.317419, whole genome shotgun sequence.
ACCESSION   DAOSWN010000033 DAOSWN010000000
VERSION     DAOSWN010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7386
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6500_length_7386_cov_4.317419"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            395..2704
                     /gene="ppsA"
                     /locus_tag="PLF29_02245"
     CDS             395..2704
                     /gene="ppsA"
                     /locus_tag="PLF29_02245"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013468444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HXK52782.1"
                     /translation="MNKNTAHTLWFNQIRKNDIALVGGKGANLGEMYHAEIPVPNGFV
                     VTAKAYFDFLKKTSLKDKILTELSGLNVEDSNKLIEASKHIKTAIMSAAMPEDIQQEI
                     KKSYHELCGEFDKRVAVRSSATAEDLPDASFAGQQETYLNVEGYKDVIKHVQMCWASL
                     FESRAIFYRTDKNYDHLKVGIAVPVQLMVQSEISGIMFTVNPLTNSRKEVSIEAAFGL
                     GQPIVSGELTPDQFLIDKKTNKITYRHIAKQTWQFTLAGKTPVSKAYQEKQKLSDKYV
                     KELAQLGMRIEDHYGRPQDIEWALEGKKLFIVQSRPVTTLNDVKADSLHSHVDEKKAG
                     KFILEGLAASPGVASGPVVIVRSPKEISRVKEGDILVAVMTDPDYVPAMKRASAIVTD
                     EGGRTSHAAIVSRELGIPAVVGSEHATKILKQGEVVTVDGSTGKVFEGDFVVKEEVKS
                     EAKADFKTATKIYVNLAEPELAEDMAARNVDGVGLLRAEFIVAQIGKHPRKFIEDGKQ
                     DEFVIKLAEGMGKFAKAFHPRPVVYRATDFRTNEFRNLVGGAKYEKDERNPMIGFRGA
                     SRYLEDDDVFRLEIEAMKIVRNKQGLKNLHMMIPFVRTVDQLVGVKKIMSANGLRRSG
                     SFMIWMMVEIPSNVILLEDFLNEGIDGVSIGTNDLTMLILGVDRDNPKVANVYNELDP
                     AVMAALEKVVTVSKKMKVTCSMCGQAPSQYPELVEKLVEWGITSVSLSPDVVEKTRKI
                     VYEAEKKLLAKKHSQKSVSKEAKRKRFTI"
     gene            2715..3995
                     /gene="eno"
                     /locus_tag="PLF29_02250"
     CDS             2715..3995
                     /gene="eno"
                     /locus_tag="PLF29_02250"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012341.2"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HXK52783.1"
                     /translation="MPKIKDITCHKVLNSRSEWTIETRVELDDGAVGTQTVPDGASTG
                     ENEALHLEAQKAAAVVNDPLKDLLVSKNPFNQLEIDTLMIDMDGTPNKRVLGGNSILS
                     VSLAVSNAAAKSLNLQLFEYLHVLYKNNSVFKPAYSKMGSINFPTPVFNILNGGKHAD
                     NDLSFQEFMVIPAKTTPYPKQLEMGVFVYNSLHKLLVSQGYESGVGDEGGFAPQGFTT
                     KKALTTIKQAVEEVRVDGEKNNRLVLGKDLFLGMDVAAESFHVGDRYEIKEESLKLDH
                     AQLSSYYGDLLQQYPIIYLEDPFYENDIEGWEHFYQNYKQKVMIVADDLVVTNAKYLK
                     AAIKKNLASAVIIKPNQVGTVSETLDFIRLAKSADMSTIVSHRSGDTPEDTFISDLAV
                     AVEADFIKSGSPARGERVAKYNRLLDIFDNFFGS"
     gene            complement(4071..4829)
                     /locus_tag="PLF29_02255"
     CDS             complement(4071..4829)
                     /locus_tag="PLF29_02255"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXK52784.1"
                     /translation="MSVTAPQTMSLVSHDQNLTPKQKTEDLMLELIENFGKDEVEEAV
                     KYLHYRNPTPLPKNEKKGKPILYVFRHGETSDNFNMLFSGWRDVNMTEKGKKQALALA
                     NKLAKKDIDMLICSDQIRSIETMKLAMSKNTKGKNLEIIKEPRIKERRYGDWEGQSKL
                     KMLLTHPESKEQRRDFDFVPPNGESMAMVEGRVLAFLREILPLMRKHNVNVALSCHGN
                     SIRAIRKKYEGLTNEQAAHIETILGEDYAAYYVG"
     gene            4875..5573
                     /locus_tag="PLF29_02260"
     CDS             4875..5573
                     /locus_tag="PLF29_02260"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HXK52785.1"
                     /translation="MSILDKLTNMLLNVANHVPVGLFTFIASFVEEVIAPIPSPIVMT
                     AAGSIAGAQNKAFIFLFWLAFLGGMGKLIGAAILYVASAKAEDFVLTRFGKFIGVSRS
                     EVEAFGQHFGKGKKDFIVIMFSRLIPIVPTAPVSILAGLSKLDFKNYMVSSFVGYLGR
                     NMIYLYIGYVGAESYVNGFDSMESIVQLMIALVLGVIVLFYFYKRKKKGSASFLSKFS
                     GVGKESATKDSSKK"
     gene            complement(5641..6270)
                     /gene="lexA"
                     /locus_tag="PLF29_02265"
     CDS             complement(5641..6270)
                     /gene="lexA"
                     /locus_tag="PLF29_02265"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008370284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HXK52786.1"
                     /translation="MSPVTLYKRQKEILDYISQYIQINGTSPTLSEIADAMGLSSLAT
                     VHEHLQTLEKKGVIKRFDGSVRGIEVLDLSVNSTPQALELPLIGYIAAGEPIEAIENP
                     LDTVLVSSDLVSKVKRCYVLQVKGDSMIGIGIFDGDYVVVQQQNVAQNGDNVVALIEG
                     NFATLKTFYREKNGKIRLQPANDSMEPIIVDEEDVQIQGKVTGVIRKFS"
     gene            6352..>7386
                     /locus_tag="PLF29_02270"
     CDS             6352..>7386
                     /locus_tag="PLF29_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HXK52787.1"
                     /translation="MFKLNSSYKPSGDQPKAIDFLLGGLEEGCRDQVLLGVTGSGKTF
                     TVANIIQEIQRPTLILSHNKTLAGQLYQEFKDFFPENAVEYFVSYYDYYQPESYIPRT
                     DTYIEKDADINENIDRMRLSATMSLMTRKDVIVVASVSCIYNLGSPAEYSKVAIELKQ
                     GMKIRLEDLLLRLNQIFYERNEFEFKRGTYRIRGSTVDIYPAYEEVGVRVELFDDRVE
                     SITRFNPLTGDPIDRSQLVSERRGSHTKSITQDESDFNSHRAKEGDFLMIYPAKHYVT
                     AEERREKAYEEIELDLKKRVAFLKAEGKAIEAYRLEQKTMYDLEMIREIGYCKGIENY
                     SRYFDGRGPGD"
BASE COUNT         2184 a         1327 c         1633 g         2242 t
ORIGIN      
        1 tttaaatgag gtgcatttaa tgattttaag tcttttggct tctcaagggg ttgattttat
       61 acctttagga gaagttgaat taaagcaagg ctagtcattt ataatttcgt tacttaaaac
      121 tctactcgcg taagctaata aatgtagttt gcccagtatg ttggtctttg cagagcaaag
      181 aaattacagc aaaacacctg cgtaagctgg cggaggttga tttccacagc gggaagacct
      241 cgtgctttag ctcggggatg aaggcgtggg ctttcaaccg cggagaaaga agagactggg
      301 gactttagtc cacggagcct tcatttagag aatattttct ttttgtacct attttttcct
      361 agactaataa atcggtatgt attaccatta gtgtatgaat aaaaatactg cccatactct
      421 ttggttcaac caaattagaa aaaatgatat agctttagtt ggaggaaaag gtgcaaatct
      481 tggtgaaatg taccacgctg aaatacctgt tcctaatggt tttgtggtaa cagcaaaagc
      541 ttattttgat tttttaaaaa aaacttcgct taaagacaaa atacttacag agttatcggg
      601 cttgaacgtt gaggattcaa ataaattgat tgaggcttcg aagcatatta aaacagctat
      661 tatgagcgcg gcgatgcctg aggatattca gcaggagatt aaaaagagct atcatgagtt
      721 gtgtggagaa tttgataaaa gagttgctgt gagatcttct gctacagcag aagatctccc
      781 cgacgcctcc ttcgcgggtc agcaagagac gtatttaaac gttgaaggct ataaagatgt
      841 aataaagcac gtacagatgt gctgggcatc gctctttgag tcgagggcaa ttttttatag
      901 aaccgataaa aattacgacc atctaaaggt tgggatagct gttcctgtgc agttaatggt
      961 tcagtcggaa atatccggca taatgtttac tgtgaatccc ctcacaaaca gtagaaaaga
     1021 agtttcgata gaagccgcct ttggattggg ccagcctatt gtgtcggggg agttaactcc
     1081 ggaccagttt ttgatagata aaaagactaa taaaataaca tatcgtcaca ttgcaaagca
     1141 gacttggcaa ttcaccctgg cgggtaaaac tcctgtctct aaagcctacc aagagaaaca
     1201 gaaattgtct gacaaatatg taaaagaatt agcgcagctt gggatgagga tagaggacca
     1261 ttacggcagg ccccaggata tagagtgggc cttggaaggt aaaaagctgt ttatagttca
     1321 atcaagaccc gtaaccacac taaacgatgt gaaagcagac tctctacatt ctcatgttga
     1381 tgagaaaaaa gcaggaaagt ttattttaga gggactggcg gcatctccag gggtagcaag
     1441 cggtccggta gttatagttc gttctccaaa ggagatatca agagtaaaag aaggagatat
     1501 actcgttgcg gttatgactg atcctgatta tgttccggcc atgaagcgtg cttctgcaat
     1561 cgtcaccgat gaggggggcc gtacttcgca cgcagcaatt gtaagccggg agttaggcat
     1621 tcccgctgta gtaggttcgg aacacgcaac aaaaatttta aagcagggag aggtagtcac
     1681 tgttgatggg tctaccggaa aggtattcga aggagatttt gtggtaaagg aagaggtgaa
     1741 gtcggaggct aaggctgatt ttaaaaccgc cactaaaatt tatgtgaatt tggccgagcc
     1801 tgagttggca gaagatatgg ctgcgcgcaa tgtagatggt gttggacttc tacgtgctga
     1861 gtttattgtt gctcagattg gaaaacaccc ccgcaaattt attgaggatg gaaaacagga
     1921 tgagtttgta ataaagctcg ctgaaggtat gggaaaattt gctaaggcct tccatcctag
     1981 accggtagtt tatagagcca cagactttag aacaaacgag tttcgtaatc tggttggagg
     2041 agcaaagtac gaaaaagatg aaagaaatcc aatgatcgga tttaggggag cgtctcgtta
     2101 tcttgaagat gatgatgttt ttcgtctaga gattgaagcg atgaagattg ttagaaataa
     2161 acagggcctg aaaaatcttc acatgatgat tccttttgta aggactgttg atcagctcgt
     2221 tggggtaaaa aagattatgt ctgcaaacgg gcttcgccgt agcggttcat ttatgatatg
     2281 gatgatggtt gaaattcctt ctaacgttat actgctcgaa gatttcttaa atgaaggcat
     2341 agatggtgta tcgataggta ctaatgatct gaccatgtta attcttggag ttgatagaga
     2401 taatccaaag gtggcaaatg tatataacga gcttgaccct gcggtaatgg ccgcattaga
     2461 aaaagttgtt actgtaagta aaaagatgaa agtgacatgt tctatgtgcg ggcaggcccc
     2521 ttctcaatac ccagagttgg tagagaagct agttgagtgg ggtattacat cagtttcttt
     2581 aagtcctgat gttgtggaaa aaacaagaaa gatagtatac gaagctgaga aaaaactgct
     2641 tgcaaaaaag cacagtcaaa agagcgtttc taaagaagcg aagaggaaaa gatttactat
     2701 ttaaggtctt gagtatgccg aaaattaaag acatcacttg tcacaaagtc ctaaactcca
     2761 ggtcagagtg gactattgaa actcgggtag agctcgatga tggtgctgtg ggaacccaaa
     2821 ctgttccgga cggagcctcg accggagaga atgaggcctt gcatttagaa gcacaaaagg
     2881 ctgctgcggt agtcaatgac cccttaaaag acttacttgt tagtaaaaac ccatttaatc
     2941 aactcgaaat tgacacttta atgatagaca tggacggcac tcccaataaa agagtgttag
     3001 gcggaaacag cattttatct gtttctctgg cagtctcaaa tgctgctgca aaaagcttga
     3061 atcttcaact gtttgagtat ttgcatgtat tgtataaaaa caattctgta tttaaaccgg
     3121 catactctaa aatgggctca ataaattttc ctactccggt ttttaatata ttaaatgggg
     3181 gtaagcacgc agacaacgat ctatcttttc aagaatttat ggtgattcct gcaaaaacaa
     3241 ccccttaccc taaacagtta gaaatgggtg tatttgttta taattcattg cacaagcttt
     3301 tagtttctca gggatatgag tcgggggtgg gagatgaggg aggctttgct cctcaaggtt
     3361 ttacgacaaa aaaagctcta acgaccataa aacaggctgt agaagaggtt agggtagacg
     3421 gtgagaaaaa caaccgttta gttctaggca aagacctgtt tttaggcatg gatgttgccg
     3481 ctgagtcttt tcatgtgggc gatcgttacg agataaaaga agaatctttg aaactcgatc
     3541 atgcccaatt atcttcttat tatggcgatc ttcttcaaca atatccgatt atttatttag
     3601 aagatccttt ctatgagaac gatattgaag ggtgggagca cttttatcaa aattacaaac
     3661 aaaaggtaat gatagtagct gatgacttag ttgtaaccaa tgcaaaatat ttgaaggcag
     3721 ctattaaaaa gaatcttgcc tctgctgtga ttattaaacc taatcaagtg ggcacggtat
     3781 cagagacctt agattttata agacttgcca aatccgccga catgtccaca atagtttctc
     3841 atcgaagcgg agataccccc gaagatacgt tcatatcaga tttagcagta gctgtagagg
     3901 ctgattttat taaatccggc tctcccgctc gtggtgaaag agtcgcgaag tataatcgtt
     3961 tgcttgacat ttttgataat ttttttggct cttaggctga tccagaaccc tttgttttat
     4021 tttttaggtc cgtgaaaaag acgaacgagt cgttggtaat ttgatcgcgg ttaccctaca
     4081 tagtaagcag cgtaatcttc tcccagtatc gtttcaatgt gagcggcctg ttcgtttgtt
     4141 agtccttcgt atttttttct tattgctctt attgaatttc cgtgacaaga tagcgcaaca
     4201 ttgacattgt gctttctcat aagagggagt atctctctta gaaatgcaag cactcgccct
     4261 tcgaccattg ccatgctctc cccatttgga ggtacgaagt caaagtctct gcgctgttct
     4321 ttactctcag ggtgagttag aagcattttt aatttacttt gcccctccca atctccgtat
     4381 cgcctttctt taattctagg ctcttttata atttccaaat tcttaccttt agtgtttttt
     4441 gacatcgcaa gtttcattgt ttctatagat ctaatttgat ccgaacaaat taacatatct
     4501 atatccttct tagccagctt attagctagt gcaagcgcct gctttttacc tttttctgtc
     4561 atattcacat ctctccaccc gctaaagagc atattgaagt tatccgaggt ttctccgtgc
     4621 ctaaaaacat atagtattgg ttttcccttc ttctcatttt ttggtaacgg ggttgggttg
     4681 cggtaatgaa gatatttcac ggcctcctcg acttcatctt taccaaaatt ttcaatcaat
     4741 tcaagcatca aatcttctgt tttttgtttg ggtgttaaat tttgatcatg gctgaccaag
     4801 gacatggtct ggggggcagt tacgctcata tattaaaaat agcacaaatg attttttctt
     4861 gctacaattc ttccatgtca attttagata aactcactaa tatgcttctt aatgtcgcga
     4921 accacgtccc ggttggtctt tttacattta tcgcctcttt tgtagaagaa gtaattgctc
     4981 caataccctc ccctattgtt atgacggctg caggatccat tgcaggggct caaaataaag
     5041 cctttatatt tttattctgg ttagcttttt tgggcggtat gggcaaactt attggcgctg
     5101 ctatacttta tgtcgcttct gcaaaagctg aagattttgt tttgacaagg ttcggtaaat
     5161 ttataggtgt ttccagatca gaggtcgaag cttttggcca acacttcgga aaaggaaaaa
     5221 aggactttat tgttattatg ttttcaagat taattcctat tgtccccaca gccccggttt
     5281 caattttggc cggtttgtca aaacttgatt tcaaaaatta tatggtttcg tcttttgtgg
     5341 gctatttagg tagaaatatg atctatcttt atatagggta tgttggagca gagtcatacg
     5401 ttaatggttt tgacagtatg gaatctattg ttcaactcat gatagctctg gtgctaggtg
     5461 ttatagtgtt gttctatttt tacaagcgga aaaagaaggg aagcgcaagt tttttgtcta
     5521 agtttagtgg tgtaggaaaa gaatccgcga caaaagatag ttccaaaaag tagttacata
     5581 atccctatga taaaaattta tagctaggtg ctggctactg atattgttgc agcagcatgc
     5641 tcacgagaat tttcttataa ctcccgtaac tttgccttga atttgaacat cttcttcgtc
     5701 tactataatt ggttccatgg aatcgttagc aggctgtagt cttatttttc cgttcttttc
     5761 tctataaaag gtttttaatg tcgcaaaatt accttctatt agagctacaa cgttatctcc
     5821 gttttgagct acattttgtt gttgaactac aacataatct ccgtcaaaaa tgccaatgcc
     5881 gatcatcgag tctcctttta cttggagtac ataacatctt tttacttttg aaaccagatc
     5941 cgaagatact agcacagtgt caagggggtt ctctatggct tcaatcggtt ccccggccgc
     6001 tatatagcct attaacggca gctctagcgc ttgtggagtg gagtttaccg acaggtcaag
     6061 tacctctatg cctctcacgg agccgtcaaa tcttttaatt acaccctttt tttctagggt
     6121 ttgaagatgt tcgtgcactg tggctagaga agaaagaccc atggcatcag ctatttctga
     6181 tagggtgggg gaggttccgt taatttgaat atactgagaa atataatcta agatttcttt
     6241 ttgtctttta taaagtgtta ctgggctcat atttaaatat taccgaaaac aaaccgaaga
     6301 tgtcaagaaa cttgcccaaa aattacccga acatatgtta taatttgttc tatgtttaag
     6361 ctgaattcat cctacaagcc ctcaggggat cagccaaaag caatagattt tttgcttggc
     6421 gggcttgagg aaggttgccg agatcaggtt ttattgggag tgaccggaag cggcaagact
     6481 ttcactgttg caaacattat tcaagagatt caacgtccca cacttatact ttcgcataat
     6541 aaaactcttg ctgggcagct ttaccaagaa tttaaagatt tttttcctga aaatgcagtt
     6601 gaatattttg taagctacta tgactactat cagcctgaaa gctacattcc tcgtacggac
     6661 acgtatatag aaaaagacgc tgacataaat gaaaacatag atagaatgag gctctcagca
     6721 accatgtctt taatgaccag aaaagacgtt atcgttgtag cctccgtctc ttgcatatac
     6781 aaccttggtt ctccagctga atattccaag gtcgcaattg aactcaagca aggaatgaag
     6841 atcagattag aggatttatt acttagactt aatcaaatct tttatgaaag aaatgagttc
     6901 gagtttaaaa gaggcactta cagaattaga ggtagcactg tcgatatcta cccggcttac
     6961 gaggaagtag gagttagggt tgaattattt gacgatcgtg ttgagagtat aaccagattc
     7021 aatcctctca cgggggatcc gatagacagg tctcagctgg tttctgagag aaggggaagc
     7081 cataccaaga gtattacgca agatgaatct gattttaata gtcatagagc taaagaagga
     7141 gactttttga tgatttaccc tgctaaacat tacgttaccg cagaggagcg acgagaaaaa
     7201 gcctatgagg aaattgagct cgacctaaag aaacgcgtag ctttcttgaa agcggaagga
     7261 aaggccatcg aggcttatag gttagaacaa aagaccatgt atgatttaga gatgatacgg
     7321 gagattggct attgtaaagg aatagagaat tactcaagat attttgatgg tcggggcccg
     7381 ggggat
//
LOCUS       DAOSWN010000034         7361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_6532_length_7361_cov_5.107309, whole genome shotgun sequence.
ACCESSION   DAOSWN010000034 DAOSWN010000000
VERSION     DAOSWN010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7361
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6532_length_7361_cov_5.107309"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PLF29_02275"
     CDS             <1..380
                     /locus_tag="PLF29_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52788.1"
                     /translation="NQEQGSVLKFTAGRQDEFSLTAPVITLKNPKQIVARPDLKYVYI
                     ADSGNNRIVIADDDGTVIAQLRLVDEEKWGDIRAIEVSSNDTKLYVLNETKIYEVNLN
                     ETLEGYIPQTQIEGPGSLPSPIE"
     gene            453..911
                     /gene="smpB"
                     /locus_tag="PLF29_02280"
     CDS             453..911
                     /gene="smpB"
                     /locus_tag="PLF29_02280"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HXK52789.1"
                     /translation="MQLAKNKKGMRDYELLENFIAGVVLTGYEVKALRDKQVNFEGSH
                     ITILDGEAYAMSITIGRYQHQSQKDTDPSRPRKLLLNKKELLKLQKELSQKGKTAIPT
                     AFILRNNMVKLEIAIVRGLKKHEKKSVEKDKQMKKELERYKKGILQPTIA"
     gene            1007..1410
                     /gene="ssrA"
                     /locus_tag="PLF29_02285"
     tmRNA           1007..1410
                     /gene="ssrA"
                     /locus_tag="PLF29_02285"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1693..5025)
                     /locus_tag="PLF29_02290"
     CDS             complement(1693..5025)
                     /locus_tag="PLF29_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52790.1"
                     /translation="MPKSFKNYKRIITTSAFFGCCVFSFLLFKKEIPNSSASLACPLA
                     DSDDGNVDGTITISGSETFSAVAGDGTIDCSGLAVVVENGGSLALEPYEDGDTDYEDD
                     DIGVTLMADSLTVEAGGRVHSDGLGYAGGVFVSYESGYGPGGGYGNSGKGSSGGGGAY
                     GGDGGDSSGLYTRPGGNQYGKPADPLLLGSGGGVGRSGGNVPSGGAGGGAFSIVLSGD
                     LVLEGEISADGLAGTQTSDTLYGSGGGSGGSIWLSIGGQLSGGGDISANGGSGGKANA
                     GNGGGGRVLVEYGTSAYTGVYEASPGLVGTTGENGSVVVVDSTSATMYIRDTQRWIPD
                     SVNSFENWFPEVQNIEISDNSSLKLQISNTAADSGSYGWEFDIQNLIVAAGSSISGEG
                     LGYQGGIFTTHENGYGPGGGQGISGSTSYGAGGGHGGAGSSSSSRVGGSAYGDNQEPV
                     TPGSGGGVGRYSSSVPSGGPGGGAVKITTREDATIDGDINFDGVMGELTIIVGRASGS
                     GAGGSVWLNVYGTLSGSGNITARGADAVDAGAGGGGGGRIAIYVAHDDFSSGTYAYGG
                     GVVSYAVPGAGTFYQYGLPYFSGVAQYYADGVTPIATAASLNETSVVLEVSMNHGSAS
                     ETLTPEVEIREVGDDFTNTATHTGDPFVYADSVVGGQVAVTGLSDLASYHWQVRFCDS
                     NSNCTSWQSYGANLESEADFTVILNAEPNIPSNLGPASYVDGSYGTDSTPALSFTLSD
                     PDVVDDLQYQIQIDDTSDFSSVLVDYTSEALSQGPQSFVVGQAEGGGSYTIGSSGQTL
                     VDAAYYWRVRAIDPSLEISPYATANSGDIAYIIDSTGPEIVGAVTGDVSKSHIVMSWT
                     TDELSTAKVWWGKASGGYTNSTIISTLSLNHQADLSNLSTDTKYNIMIVNYDAQNNAG
                     TEYLTSYTTNESTSQDKDDKDRNNNDSENTVSSNISDDTDVTFTRIQSFDYENSFKSD
                     PNESDDQGGDKALDESMQIVVDEPLYDLVVYVKDRKGNLVPDALVTIKELNISVNADN
                     NGKAVFPNVPKSVYTVSAKTPLGDVMGIVDRASFGSESEVYFELTSDRGFFSNRVVQA
                     GALTALFLVLVLILKTVLTKKI"
     gene            complement(5415..7187)
                     /locus_tag="PLF29_02295"
     CDS             complement(5415..7187)
                     /locus_tag="PLF29_02295"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK52791.1"
                     /translation="MKRFIKYSWQTNKKLYFIYSFITILLATVPIVISWVESRIIDQL
                     TGYLQSENVVRVSTALLLLILSLVSVRVIKSLLDIVEGILNLKWFFVLGQKINFDFIR
                     KASILDVAHFENNETHNLLNTSDENVNRALNLSANIVGFSYPIIGLLSSVVILASYKP
                     SIILFALVAVLPAAIIDVAFGRKSYGIWGAEGAVRRDYYKSRWYLIDAQQLLEVRVNK
                     LREFLLERVYGLYSSFEEKQTENEIKRTKYQVASSVLLFFFGLYTYLILVNSVISKEI
                     TLGEFTFYISMIGIFSGGLARLLSTFSSIYRSSLFVDGVFKVFDLENKIVPGERCLAD
                     NKIPPRIEFRNVDFFYPTSTEDNKVLVDFNLIIKPGEKVALVGVNGAGKTTIVKLLMR
                     IYDVTGGEILIDNIPLKDLSLENWYSKIGALFQEFNFYHFDAKTNIATGDPSRMDDID
                     AVVEAAKKSGAHSFIKNYEYGYDQVLSTVFEKGIIPSAGQKQKIAVARTFFKDAPILI
                     LDEPTSAIDAKAEFEIFENIYTFSQNKTVITISHRFSTVRNADRILVLEKGKILEDGS
                     HNELMLLEDGVYKKAFELQKRGYV"
BASE COUNT         2268 a         1724 c         1382 g         1987 t
ORIGIN      
        1 acaatcagga acaaggttcg gtgcttaaat ttacagcggg gagacaagac gagttttctc
       61 ttactgcccc tgtaataact ttaaagaacc ctaagcaaat agttgcgcgc cccgatctta
      121 aatacgttta cattgccgac agcggaaata acagaatagt aattgccgac gatgacggta
      181 ccgttattgc ccaacttagg ctggttgatg aggaaaaatg gggcgacatt cgcgcaatag
      241 aagtcagctc aaacgacacc aagctttatg ttctgaatga gactaaaatt tacgaggtaa
      301 acctaaacga aactcttgaa ggctatatac cacaaacaca gatcgaaggc ccggggtctc
      361 ttccctcacc tatagaataa ccggctcctc ttgctgctca tgcagaaaat aattttggct
      421 aatgttcttt acgccaatgt tattatatac acatgcaact agctaaaaat aaaaaaggaa
      481 tgagggacta cgagctgctg gaaaacttta ttgcaggagt agtacttacg ggctacgaag
      541 ttaaagcttt gagagacaaa caagtaaatt ttgagggctc gcacataacc atattagatg
      601 gagaagccta tgctatgtca atcacaatag gcagatacca acaccagagt caaaaagaca
      661 ctgatccgtc aaggccgcgt aaattactgc tcaataagaa agagctgcta aaattacaaa
      721 aagaactcag tcaaaagggc aaaactgcta tacctacggc ttttatatta cgcaataata
      781 tggtaaaatt ggaaatagca atagtaagag gtttgaaaaa acacgaaaaa aaatctgttg
      841 aaaaagacaa acagatgaaa aaagagctgg agagatacaa aaaaggcata cttcaaccta
      901 ctatcgctta atgtgattcc agaaaaacta tctagaacgt tattaaataa agccgttctt
      961 ttggtagctt cagaaatatt ctgcggaatc actaatcata tctaacgggg atgccaggat
     1021 ttgattggaa gtgtcaggct atttaagcaa gctgcgttgg cggatcgcat gaaaccgtca
     1081 aaaaataaat gcagaagata attcaattgc atatgctggt gtaggagctt acgcccctgc
     1141 aatggcatta gtctaagttt taaactaaga caggttcact tatatttacc cggaaatgta
     1201 agttgaatct aatagaaccg ggttgcggta gatattaagc agtcgttatg tttaccaagc
     1261 acaaaagtgt aaagccgaaa ggttagacta acaaaaggac aaacgcggta tgccaaaacc
     1321 gtaccttttt gaaagaattt ggcgaagctt gtaataactt gggtagttcg atctttcaag
     1381 acgggggttc gattcccccc atctccacca aactggaatt tccagttttt aatgcatccg
     1441 aaagatggaa gcgcaatttt ttttctgatt agcaatccag tttcgagaca acagctagct
     1501 ccttctggga gtatctattc tcaaccgttg cctgaacaaa atacatggcg tggcctgggg
     1561 ccgaatttag aacagtaaaa gcccaacata ttgggtaaac tacaccaact aacttacgta
     1621 ggtagtttta aataacgaaa ctataaaaag cggaaagaga ttttcttttt aatttaactc
     1681 ccggagaaga ttttatatct tcttcgtcag aaccgtttta agaattaaga ccaacactaa
     1741 aaatagtgcg gtaagtgccc ctgcctgaac aaccctattt gagaaaaacc ccctatcaga
     1801 agttaattca aaatatactt cactctcgct tccaaaagaa gcgcgatcaa caatgcccat
     1861 aacatcccct aaaggagttt tggcactaac tgtatagaca gatttaggaa catttggaaa
     1921 cacggccttg ccgttattgt ctgcatttac actaatattc agctctttta ttgtaactag
     1981 ggcatcgggc actagattac cttttctatc ttttacatat accaccagat catacagagg
     2041 ctcatcaaca actatctgca tgctctcgtc caacgcctta tcgccgccct gatcatcaga
     2101 ttcatttgga tcgctcttaa agctattttc gtaatcaaac gattgaattc gagtaaaagt
     2161 tacgtctgtg tcgtcagaaa tattagatga aactgtattc tccgagtcat tgttgtttct
     2221 atccttatcg tctttatctt gagacgtact ctcattagtg gtgtatgagg ttaaatactc
     2281 agtacctgcg ttattttgag catcgtagtt cacaatcata atattatatt ttgtatcggt
     2341 agataaatta gataagtcgg cttggtggtt taagcttagt gttgaaatta ttgtactatt
     2401 tgtatagcct ccgcttgctt tgccccacca cactttagcg gtgctaagct catcagtagt
     2461 ccacgacatc actatatggc tcttcgacac atctccggtc acagcaccaa caatttcggg
     2521 gcctgtcgag tctattatgt acgcaatatc tcccgaattg gccgtggcat agggagaaat
     2581 ctctaagctt gggtcaattg ccctaactct ccaataatat gcggcgtcaa cgagagtttg
     2641 tcctgagctt ccgatagtat aactgccccc tccctcggcc tggcccacaa caaaagattg
     2701 cggaccttgt gaaagagctt cagaagtata atctacgaga acagagctga aatcggatgt
     2761 atcatctatt tgaatctgat actgcaggtc atcaacaacg tccggatcgg acaacgtgaa
     2821 acttaacgcc ggagttgaat ctgtaccata actgccgtct acataagagg cagggcctag
     2881 attagaggga atgtttggtt ccgcatttaa tattactgta aagtccgctt ctgattccaa
     2941 gtttgcgcca taactttgcc atgatgtgca atttgagtta gaatcgcaaa aacgtacctg
     3001 ccaatgatag gaggctaaat ctgataaacc tgtgacggcc acttgcccgc caactactga
     3061 gtcagcataa acaaaagggt ctcccgtatg ggtagcggta ttagtaaagt catcaccaac
     3121 ttcacgaatt tcaacctcag gcgtcaatgt ttctgaagca ctaccatgat tcatgctaac
     3181 ttcaagaacg accgaagttt catttaatga tgcggcggta gcaattggag ttacgccgtc
     3241 agcgtaatat tgagcaacgc ctgagaaata aggaaggcca tattgataaa aggttccggc
     3301 tccgggcaca gcatagctaa ccaccccgcc cccataagcg taagtacctg aagaaaaatc
     3361 atcgtgagca acataaatcg ctattctgcc gccacctccg ccccctgcac ctgcgtctac
     3421 tgcatcagca ccgcgagcag tgatgttccc ggatcctgac aaagttccgt acacattcaa
     3481 ccagacggag cccccggctc cgcttcctga agcacggcct actataatag tcagctcacc
     3541 cataacgccg tcgaagttta tgtctccatc aatagtagca tcctcgcgag tagtaatctt
     3601 tacagcgccg cccccggggc ctcctgaagg cacgctactt gaataacgcc ccactccgcc
     3661 gccacttccc ggagtaacgg gctcctggtt gtctccataa gccgagccgc caaccctgct
     3721 cgaagaactc gagcccgctc cgccgtgacc gccccctgca ccatagcttg tgctgcctga
     3781 aatgccctgc cctcctccag gaccataacc attttcatgt gttgtaaaaa taccgccctg
     3841 ataacctagc ccttctccag aaatactact acctgctgca actataagat tttggatgtc
     3901 gaactcccaa ccatagcttc ctgaatctgc ggcagtatta ctaatttgca attttaaaga
     3961 agaattatca gatatctcta tgttttgaac ttcaggaaac cagttttcaa aagaattaac
     4021 ggaatcagga atccatcttt gagtatctct aatgtacatg gttgccgacg tgctgtccac
     4081 aactacaacc gagccatttt caccagtagt tccgactaat ccgggggaag cctcataaac
     4141 gcctgtatag gctgaggttc cgtattcaac caagactctc ccgccaccgc cgttaccagc
     4201 gttcgctttg cccccgctac caccgttagc agaaatatct cctccgcctg aaagttggcc
     4261 cccaatagaa agccagatcg atcctccgga acctcctcca gagccatata atgtatcgga
     4321 cgtctgggta ccggcaagtc catcagcgga aatctctccc tccaacacca aatctcccga
     4381 cagaacaatg gaaaaagctc ctccgcccgc ccctcctgaa ggcacgttgc ctccggacct
     4441 tccaacaccg ccgccactac ctaacaatag ggggtcggcg ggctttccat attgatttcc
     4501 gccggggcga gtataaagac cagaggaatc tcctccgtcg ccgccataag ctcctccgcc
     4561 accactgctg cctttaccag agttcccgta acccccgccg ggcccatagc cggactcata
     4621 tgaaacaaaa accccgcctg cataacccaa accatcagaa tgtactctgc ccccggcttc
     4681 aactgttaag ctgtcggcca tcaatgtaac tccgatgtcg tcgtcttcgt agtctgtatc
     4741 accgtcttcg tagggctcca acgctaaaga cccgccattt tcaactacaa cggccagacc
     4801 actacaatca atagtgccat ctcctgctac tgcagagaaa gtctccgatc ctgaaatggt
     4861 tatagtgccg tccacatttc cgtcatcgct gtcagctaaa ggacaggcca gagatgcgct
     4921 agaattagga atttccttct taaatagcaa gaaagaaaaa acacaacagc caaaaaaagc
     4981 tgaggtggtt attattcgtt tatagttttt aaatgacttg ggcacaatca atctcttata
     5041 agttgcaact attgttctaa gtatgacata aaaaaaggat attcaaaaac gagccgcctt
     5101 gaaaactaca aaagcggctt tttatgacag agaaacttat tatcaaccac caccagctta
     5161 cgcaggtggc ttgctgtaat ttctttacgt tgcaaagacc aacatactgg gcaaactaca
     5221 tctactatct tgcgtaaata gtattaagta acgaaattat caaccaccac cagcttacgc
     5281 aagtagtttg ctgtaatttc tttaagttgc aaagcccagc atgttgggca aactacatct
     5341 actatcttgc gtaaatagta ttaagtaacg aaattataaa aaacgagttc tgggggcaaa
     5401 aatatcccac aaagctaaac ataacccctc ttctgcaatt caaaagcttt tttatacaca
     5461 ccgtcttcta aaagcatcag ctcattgtga gaaccgtctt ctaggatttt gcccttttct
     5521 aaaacaagaa ttcggtcggc attacgaaca gttgaaaatc tgtgggatat agttattact
     5581 gttttattct gcgaaaaagt ataaatattt tcaaaaatct caaattcagc cttagcatca
     5641 attgcactag taggctcatc tagtataagg attggggcgt ctttaaaaaa agttcttgct
     5701 acagcaattt tttgtttctg gccagctgag ggaataatgc ctttctcaaa aacggtactt
     5761 aaaacctgat catacccgta ctcatagttt ttaatgaagc tgtgagcacc cgactttttc
     5821 gctgcctcaa ctaccgcatc tatatcgtcc attctcgaag gatctcctgt tgcaatattc
     5881 gtttttgcat caaaatgata aaaattaaat tcctgaaaca aggcgccaat ttttgagtac
     5941 cagttctcca aactcaaatc ttttaatgga atattgtcta ttaagatttc tcctcctgtt
     6001 acatcgtaga ttcgcatgag tagcttgact atggtggttt ttcccgctcc gtttacacca
     6061 accaaagcaa ctttctcacc gggtttaata atcaaattaa agtcaacgag aaccttgtta
     6121 tcttctgtag aagttgggta aaaaaagtct acatttctaa actctatacg cggaggaatt
     6181 ttgttatctg caaggcacct ctcgcccgga acaatcttat tttcaagatc aaaaacttta
     6241 aaaaccccat caacaaaaag gctactacgg taaatacttg aaaaagtact caaaagacgt
     6301 gctaaaccgc cagaaaaaat acctatcata ctaatataaa aggtgaattc ccccaacgtt
     6361 atttctttag agattactga attaacgaga ataaggtacg tataaagccc aaaaaagaat
     6421 aataaaacac ttgatgcaac ttgatattta gtccttttaa tttcgttttc tgtttgcttc
     6481 tcttcaaatg aagaatacag gccataaacc ctttcaagca gaaattctct aagtttgttt
     6541 actctaacct ctagcagctg ctgggcatct attagatacc atcttgattt gtaatagtcc
     6601 cttctgactg ccccttctgc accccaaata ccgtaacttt tcctaccaaa agctacatca
     6661 ataatggccg caggcagaac cgccaccaag gcaaataaaa taatggaagg tttatagctt
     6721 gccaatataa caacgctaga cagcaaacct ataattgggt aggaaaaccc tactatgtta
     6781 gcggataagt tcagtgctcg gttaacattt tcatctgagg tgtttaaaag attgtgagtt
     6841 tcattgtttt cgaaatgggc cacatctaag atagacgctt tccttatgaa atcgaagtta
     6901 atcttttgac ccagcacaaa gaaccatttc aaattaagaa tgccctcgac aatgtctaac
     6961 aggcttttga taacacggac cgagactagg cttaaaatta acaataacag ggctgtgctt
     7021 accctaacaa cattttcgga ttgtaaataa cctgtaagct ggtcaatgat acgactctcg
     7081 acccaagata taacaatggg caccgtggcc aataaaattg taataaagga atatataaaa
     7141 tataactttt tatttgtttg ccaagaatac tttatgaacc ttttcaccgt acgaaatact
     7201 agcaacgttt cttcgcgaaa ccttgttgtt aagatgttgc taaaaatttt gtttttgtcg
     7261 tatttgctaa acataatctg gctgtgcttt tgagtccacc tgttgccttt tactgtttac
     7321 tgttgtgcgg aaagaaaaaa ggcaacaata aaagtataca g
//
LOCUS       DAOSWN010000035         7313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_6605_length_7313_cov_5.152521, whole genome shotgun sequence.
ACCESSION   DAOSWN010000035 DAOSWN010000000
VERSION     DAOSWN010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7313
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6605_length_7313_cov_5.152521"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(280..432)
                     /gene="rpmF"
                     /locus_tag="PLF29_02300"
     CDS             complement(280..432)
                     /gene="rpmF"
                     /locus_tag="PLF29_02300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HXK52792.1"
                     /translation="MPRPKRKHSKGRVRRKRDEKMQQQVVQTVQCEHCKAQKLPHRVC
                     DSCGKE"
     gene            complement(658..2205)
                     /locus_tag="PLF29_02305"
     CDS             complement(658..2205)
                     /locus_tag="PLF29_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52793.1"
                     /translation="MSLLKPFMISTEPTDANKVEQTFGLSGDQGSLTIRTEKGGPGKH
                     GAVTIIFNVKDGSEEGKTVILKKQGDGTSYIGSRENLLAELETGREREILAEQSPNFN
                     TRVAQLLRQLPFPDVLNRRLPKILKLNGDLALLETLPHAEALMALLVLAKTDLDAGRI
                     QYPESPLKAEHNILTIIDIYKRCGPETARLYYNLTREIETLPQYAIERIAKLQDLGVP
                     SIYSLVRGFIKMSDSAGLKDRDEEQTALLNRLLVAAGANISRIITPKDKARFPALAVC
                     EKTAKDSLLDVASTTTRHDRNGPDEPCFPFFARKLEEQGEQGEQPQNTEVTHLYGASC
                     DGKACNVDIAARWIPELNTADYVEATLSHTTVANAGYDTGIKSERRVVQVITVSTEGV
                     RIKLAEKEGDFHTLIISKGDVEITMRHKYKPDRTGWEYKRTVSLDGGSTYMRDVNNPT
                     PEQQQDLSFLDLDLIEGLFISNPRSQPHAIEDVALVAGEPKKENPAQRVEKIVKALLL
                     PNTVRNT"
     gene            complement(2400..2957)
                     /gene="rsmD"
                     /locus_tag="PLF29_02310"
     CDS             complement(2400..2957)
                     /gene="rsmD"
                     /locus_tag="PLF29_02310"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HXK52794.1"
                     /translation="MDITTRVTSGTAKNRKLKIPDIEGIRVHKDVFKQALFNIIGEKI
                     VNANCLDLFAGSGSAGIEALSRGAKHCDFVDHNKRAITTIQENLSTCGLQDLAQIFHS
                     DCIKYIANIYKKYNVIFCDPFYSDLKHKFLLKNLAEILEKGGLIAFSHSKDLNLEEQL
                     IGTSLKILQSRKYGESFLSIIGFGK"
     gene            complement(2961..4979)
                     /gene="recG"
                     /locus_tag="PLF29_02315"
     CDS             complement(2961..4979)
                     /gene="recG"
                     /locus_tag="PLF29_02315"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HXK52795.1"
                     /translation="MHKISSNVATIAKIGPKYKDLLAKLGIYSIEDLLYHRPIRYEDY
                     SKITNIAELEPDKNVTIKAKINKIDNIYTKTGKRLTKAVVGDSSGKVEVVWFNQQYIA
                     KSIKLENEYYFSGKVQVFNNKISMPSPQFEEIDKQGVSTARIVPIYPETAGLTSKWLR
                     SRINDILPKLLEEGAIGEYLPEKLLSEYNLLDLPTALCHLHMPEGTQDISQAEKSLAF
                     RELVIELLKVQKTKDTWKSAFSAYRLDYKKLDVSNFTKNLGFSLTNSQEKAIEEILND
                     TDRERPMNRLLEGDVGSGKTIVAVAAAYNTLLNGHSTIYMAPTGILAQQHYSTFKKFL
                     EPLGIKVLLITGSEKQPIEESTPSIIIGTHALLHLKKGVKNLGLVIIDEQHRFGVEQR
                     SKVMGTNEIKPHLLSMTATPIPRTLALTIYGHLEISTLDTVPNTEKKITTKVISESQR
                     NKVFEWIRKRNEPTFIVCPFIESSTNEDFENIRAAETEFQRLQQGSLKGKAVGLLHGR
                     MSEKEKNQTLDNFRTGKIKFLISTPVIEVGVDIPEATIIVIESAERYGLASLHQLRGR
                     VGRGAKEGFCFLFMSGYSKNSYTRLKNLEITSSGLKLAEIDMKYRGQGNIYGTQQHGF
                     KNFKYADFSDLKAVRTAKKAAEELYGQLEDLPNLKQKLDEHASMAVSN"
     gene            5164..6000
                     /locus_tag="PLF29_02320"
     CDS             5164..6000
                     /locus_tag="PLF29_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52796.1"
                     /translation="MNLEPSFSPKGTRERHYEFNGADGDLFGFTVDHIEQVNPSRGYM
                     SALSRAEIGKGSASLTIEVVHHAGFPRSNLIDAQGADERGLISLPFSSLFFALKVSRP
                     LQAVEIDYLNQSAPGDTAEIISAHRAFLLQGNLVPLITPVSGIHALEITRKDNKTGFY
                     PQPGSGFHGYSAYIPTRAFQGYVVTTIDPALMRRLFHPVYESSRIVGADTICGSTYVR
                     PINIIRPDQLYLLDSQAFWNLLPAFRDGGILYGREKPRQNIPLNIRELTFPVCVLPDL
                     KF"
     gene            6186..6761
                     /locus_tag="PLF29_02325"
     CDS             6186..6761
                     /locus_tag="PLF29_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52797.1"
                     /translation="MAIFEGETPYTNSTRFELIEQGTGGVKIIVYIAGKAGVGLEIEA
                     EILRFYGPSPAVARGGDLFVSSVRTTTGFGLEIFSPKAVLPATTGSSGPFQLRPKILE
                     LPLWFTQDKGKDTARSNNHFDVTYLSILATQPEASLVGYDENLRRWRFNDPKVKVNVG
                     LFNQIILQRQHDEIFYRDGLPPRKYILQRNI"
     gene            complement(7022..>7313)
                     /locus_tag="PLF29_02330"
     CDS             complement(7022..>7313)
                     /locus_tag="PLF29_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52798.1"
                     /translation="STEDAKTAARVFEETVQNKEISEELFTQSTSTGSQTILDFIRKE
                     LGNKFSSGEIKRTISQGGLELNGNKVTEPNSVHKFIKGDIIKFGKRTFIRLE"
BASE COUNT         1877 a         1458 c         1392 g         2586 t
ORIGIN      
        1 atcctcggcc gtctctatgg tattttcgat agagtaggtt gagtctggat ccgcctcctg
       61 ttgctggggc atgctaaata gtactgctac cgcgagtctt ctggcgctgt gtctgggatc
      121 tttagtgttt ttcatcggca gttgcccttg ttaatttttg tacgcagacc atatttcatg
      181 aaactaaata gctgtattta gttcaattct tctctcatat tgccatttta agtgcaattt
      241 ggctctgtat tagtttgcga tgtaacagaa cagaacagac tattccttac cgcaagagtc
      301 acaaactctg tgagggagtt tttgagcttt acaatgctcg cactgtacgg tttgtacaac
      361 ttgttgctgc atcttttcgt ctctttttct tcggactctt cctttactat gttttctttt
      421 aggtcttggc ataccctagt aaagtaccac tataaggatg aattagcaag aggttttgag
      481 agcttggtat gcgagaagaa ctggggcggg ggttttagcg gcttgagtca tttactcgtt
      541 tgtcaagaag atgaaatttt ttcgttgatt cggtgttgcc agcgcagagt tccatgtact
      601 ggaatatttt tttgcgtagc ttatcgtttc ttagtctgta tgggacctta agggcaccta
      661 tgtgttacga actgtattag gtaataataa ggctttaact attttttcca ctctttgagc
      721 gggattttct tttttaggtt caccggctac taatgctaca tcttcaatag catggggctg
      781 cgatcgtgga ttgcttataa ataaaccctc tatcaaatct aaatctaaga aagaaagatc
      841 ttgttgttgt tcaggagtag gattatttac atctcgcatg tatgtactgc caccgtctaa
      901 agataccgta cgcttgtatt cccagccagt tctatctggt ttatatttgt gtctcattgt
      961 aatttccaca tcacctttag atataatcag tgtatgaaaa tccccctctt tttcagcgag
     1021 ctttattcta acaccctctg tacttactgt aataacttgc actactcttc tttctgattt
     1081 aataccagta tcatacccag cattggcaac ggtggtatga gaaagagtcg cttccacata
     1141 atctgctgtg tttagttctg gtatccaacg agcagcaata tctacgttac acgcttttcc
     1201 atcacagctg gccccataaa gatgagtcac ctctgtattt tgaggttgtt caccttgttc
     1261 accttgttcc tctaactttc tggcaaagaa agggaaacaa ggctcatccg gaccattcct
     1321 gtcatggcga gttgtcgtag aagctacatc aagaagtgag tccttggccg ttttctcgca
     1381 cacggcaagt gccggaaatc ttgccttatc cttaggggta attatacgag aaatatttgc
     1441 tccggcggct acaagcagtc tatttaacag tgcggtctgt tcttcatccc tgtctttaag
     1501 tccagcgctg tcagacattt tgataaaacc tcgtactaga gagtatatag aaggtacgcc
     1561 caaatcctgt aacttagcaa ttcgttctat cgcatattgc ggcaaagttt ctatttccct
     1621 tgttaaatta taatacaatc ttgctgtctc agggccgcac cgcttataaa tatctatgat
     1681 tgtcaaaata ttgtgttcag ctttgagcgg gctttctgga tactgaattc tgccagcatc
     1741 aaggtctgtt tttgcaagta caagcagggc cataagagcc tctgcgtggg gtagggtttc
     1801 gagcaaagca agatctccat tgagtttcaa aattttgggt aaccttctat ttaaaacatc
     1861 gggaaatggc agttgccgca ataattgagc gactctcgtg ttaaaattag gactctgttc
     1921 ggctaaaatt tctcgctctc tacctgtttc gagttcggca agcaggttct cacgagaacc
     1981 aatataggat gtcccgtcgc cctgtttttt tagtattact gtcttgcctt cctccgatcc
     2041 atccttgaca ttaaaaatta tggtaaccgc tccgtgtttt cctggtcccc ccttttctgt
     2101 gcgaatagtg agggagcctt ggtctccact tagcccaaag gtttgttcta ctttattagc
     2161 gtctgtgggt tcagtagaga tcataaaagg ttttaataaa ctcatgttct cattgtaaca
     2221 agaacaattt atgctgtaaa cctctcttga aactaagtct gttgcgtaag tgccgtaaga
     2281 ccatagaaca aatttgtagt tagcaccctt gctgctgtaa gatagagaca cccctctcgt
     2341 taaataaggt cttagttcac aacagtgaga aaaaatttca gatatagctt tatagtatct
     2401 tatttcccaa aaccaataat gcttaaaaaa ctctccccgt attttctgga ttgcagaatc
     2461 tttaggcttg ttcctataag ctgttcctcc aaattcaggt ccttggaatg agaaaaagct
     2521 atcaagcccc ccttttctag tatttccgct aaatttttta ataaaaattt atgtttcaaa
     2581 tcgctataaa aaggatcgca gaaaataaca ttgtattttt tgtatatatt agcaatgtat
     2641 tttatgcaat cgctgtgaaa tatttgtgct agatcctgaa gtccgcatgt tgatagattt
     2701 tcttgaattg tagtaattgc tcttttgtta tgatctacaa aatcgcaatg ttttgcccct
     2761 ctgcttagcg cctcaattcc ggcactacct gagcccgcga aaaggtctaa acagtttgca
     2821 tttactattt tctcaccaat aatgttgaaa agggcttgtt taaaaacatc cttatgaacc
     2881 cttattcctt cgatgtcggg tattttcagt tttctatttt ttgcagttcc tgacgtgact
     2941 ctagttgtaa tatccatgtg ttaattagat acagccatag aggcatgttc gtcaagtttc
     3001 tgtttcaaat taggtaagtc ctccagttgg ccgtaaagct cttccgccgc ctttttagct
     3061 gttctaacgg ctttcagatc actaaaatcg gcgtatttaa agtttttaaa tccatgttgt
     3121 tgggttccgt aaatgtttcc ttgacctctg tatttcatat ctatttctgc cagttttagc
     3181 cccgagcttg tgatttctaa attttttaat cttgtatagg aattttttga atatcccgac
     3241 atgaacaaga agcagaatcc ttcttttgct cctcttccaa cccttcctcg tagttgatgc
     3301 aatgaggcca gcccataacg ttccgcactt tctatcacaa ttattgtagc ctcgggaata
     3361 tcgactccca cttcaatcac cggtgtcgag attaaaaact ttattttacc tgtgcgaaaa
     3421 ttgtctaagg tttgattttt ctctttttca ctcatacgcc catgcaacaa cccaacggcc
     3481 ttgcccttta gactgccttg ttgaagccgc tgaaactccg tttcagcggc cctaatattc
     3541 tcaaaatcct catttgtgga agattcaata aatgggcaaa ctataaacgt aggttcgttt
     3601 ctctttctaa tccattcaaa taccttattt ctctgagact cggagattac ttttgttgta
     3661 atcttttttt ctgtattcgg tacagtatct aaagtagaaa tttcaagatg tccgtatata
     3721 gtgagtgcta aggttcgagg tatgggtgtt gccgtcatag acaaaagatg gggttttatt
     3781 tcatttgttc ccatgacttt tgatctttgt tcgactccga atcggtgttg ttcgtcaata
     3841 attacaagtc ccaaattttt gacccctttt tttaagtgaa gtaatgcgtg agttccaatt
     3901 attatgcttg gtgtgctttc ttcgataggc tgtttctcgc tgcccgttat aagcagaact
     3961 ttaatcccca aaggttctag aaatttttta aatgttgaat agtgctgttg ggctaaaatt
     4021 cctgttggcg ccatgtatat tgtgctgtgc ccgtttagta aagtattata tgctgctgca
     4081 acagcaacaa tggttttacc tgagccaacg tccccctcaa ggagtctgtt cataggtcgt
     4141 tccctatccg tgtcatttag aatttcttct atagcttttt cctgggaatt tgttaatgaa
     4201 aacccaagat tttttgtaaa gttagataca tcaagttttt tataatcaag cctgtatgct
     4261 gaaaaggcgc ttttccacgt atcctttgtc ttttgaactt taagcagctc tataaccaac
     4321 tctctaaatg ctaaactctt ctctgcttgg gagatgtctt gtgtaccttc tggcatgtgc
     4381 aggtggcata atgcggtagg caggtctaga agattgtatt ctgaaagtaa tttttccggc
     4441 aaatattccc ctatagcccc ttcttctaaa agttttggta aaatgtcgtt aatcctagac
     4501 ctcagccact ttgaagttag gcctgctgtt tcaggataaa ttggtacaat ccttgcagta
     4561 ctaacccctt gtttatcgat ttcttcaaat tgtggagaag gcatagatat tttattattg
     4621 aagacttgaa ctttcccgct aaagtaatat tcattttcta gttttatgct ttttgcaata
     4681 tattgttgat taaaccaaac aacctcaact tttcccgatg agtcccctac tacggccttc
     4741 gtaagacgtt tgccggtttt ggtataaata ttgtctattt tgtttatttt ggcctttatg
     4801 gtcacatttt tatctggttc cagttcggca atattggtta tttttgaata gtcttcgtat
     4861 ctaatcggtc ggtggtaaag taaatcttct atggagtata taccgagttt tgccagcaaa
     4921 tctttgtatt ttgggccgat ttttgctatt gtagccacat tagagcttat tttatgcata
     4981 ttattatttt atcgtatcat ttaattttat aatttcgtta attaaaacta ctcgcgttag
     5041 ctagtagatg cagtttactc aacatgcggg gctttgcaac ttaaagaaat tacagcaaac
     5101 cgcccgcgta agctggcggt agttgattat tttaattaaa ttgtactgtg attttaggat
     5161 tttatgaatc ttgaacctag tttcagtccc aaagggacga gggaaagaca ctacgaattt
     5221 aatggcgccg acggtgacct ttttggtttt acagttgacc acattgagca agtaaatcct
     5281 tcacgaggtt acatgagcgc tttatcaaga gctgagatcg gcaaaggctc tgcctcctta
     5341 actatcgagg tggttcatca cgcaggcttt ccccgctcaa atcttattga tgctcaaggg
     5401 gcagatgaga gaggtctaat ctcactacct ttttcctcgc tattctttgc attaaaggtt
     5461 tctcggccct tgcaggctgt tgaaattgac tacctaaatc aaagtgctcc tggagataca
     5521 gcagaaataa ttagtgcgca tcgagcgttc ttgttacaag gaaatctagt tcccctcatc
     5581 acgcccgtct cgggaattca tgcattggaa ataactcgca aagacaataa aacaggattc
     5641 taccctcaac ctggatctgg gtttcatgga tattctgcat acattcccac aagagctttt
     5701 cagggctatg tagtgacaac tatagaccct gctttaatgc gtagactttt tcatccagtt
     5761 tatgaaagct ctcgcattgt tggagccgat acgatttgcg gttctacata tgtaagaccc
     5821 ataaacatta ttaggcccga tcaattgtat ttacttgaca gtcaagcatt ttggaatttg
     5881 ttgccggcgt ttcgtgatgg cgggatatta tacggtcgtg agaaacctag gcaaaatata
     5941 cccctaaata ttagggaatt gacttttcca gtttgtgttt tgcccgatct gaagttttaa
     6001 tagctgcagt acctttaaga ttagtttttc ggttattgtt agtacggctc ttgtaattat
     6061 aaaattatca attaattcta ttttctttct tttttaataa gattttgccc ataagaaatt
     6121 ttgagggttt ggggtttagc ttttttctct ttcttattat tcttctgtta taattctaac
     6181 gttatatggc aatttttgaa ggtgaaactc cttatacaaa tagcaccaga tttgagttaa
     6241 tcgagcaagg tacaggtggt gtcaaaatta tagtttatat tgctggtaaa gcaggggtgg
     6301 ggctagaaat agaggctgaa attttgaggt tttatggccc cagcccagct gttgcaagag
     6361 ggggcgattt gtttgtttcc tctgtaagga ccactactgg tttcgggtta gaaattttct
     6421 cacctaaagc tgttttacct gccaccactg gttcttccgg cccttttcaa ttgcgtccaa
     6481 aaattctgga actgcctctc tggtttactc aagataaagg taaggatact gctcgatcta
     6541 acaaccattt cgatgttact tatttatcaa ttttagcaac tcaacctgaa gcaagtcttg
     6601 ttgggtatga cgaaaatctt cgaagatggc gctttaatga tcctaaagtt aaagtaaacg
     6661 tgggtttgtt taatcagatc attttacaac gccagcatga cgagatattt tatcgtgacg
     6721 gtttacctcc ccgaaaatac atcttgcaaa ggaatatttg agattgcttt aaatcacata
     6781 aggcaataca ctcgtagcaa taatactcaa agtgcaaaga ataacaataa acttaacaag
     6841 attttttttg ttaaagatcc gcttaagttt tttgcttcta taaaacttaa actttttttt
     6901 atgtcccggt aatgatatta gatccataac gcccctgagc gtgggtagtt ttttattttc
     6961 ttttaagatt tttttaaaca ctgcgctatt cctataaata aattttttcg tagttttatg
     7021 actattccag tcttataaaa gtacgttttc caaatttaat aatatcgcct tttataaact
     7081 tatgcactga gttgggttct gttactttgt tgccattcag ttcaagtcct ccttgagata
     7141 ttgttctttt tatttctcct gacgagaatt tatttccaag ttcttttctt ataaaatcaa
     7201 gaatggtttg acttccggtg cttgtgcttt gagtaaaaag ctcttctgag atttctttat
     7261 tttgcacagt ttcttcaaag acccttgctg cggtttttgc gtcttctgtg gag
//
LOCUS       DAOSWN010000036         6628 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_7570_length_6628_cov_4.575080, whole genome shotgun sequence.
ACCESSION   DAOSWN010000036 DAOSWN010000000
VERSION     DAOSWN010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6628
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7570_length_6628_cov_4.575080"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PLF29_02335"
     CDS             complement(<1..200)
                     /locus_tag="PLF29_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52799.1"
                     /translation="MDLHDDTIYTESKAKFTCPSCGDVSQDDVFFLCNRCERSELIYK
                     DGLYICPACLKPGENFECMKCGS"
     gene            440..1180
                     /locus_tag="PLF29_02340"
     CDS             440..1180
                     /locus_tag="PLF29_02340"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HXK52800.1"
                     /translation="MYKSLLQNKKAVIFDLDGTITDTEYFWAKAIVNIADENYGFVEY
                     NIAETAGLELTDRWEHILLKRQIEPKIPYSDLVKMTITRFVQYITEAETIKTLPGFWS
                     LATELKLEKNIKIGLTTNTRKELVNTILDKIGLSEFFDAVVTLDDAKKPKPDPEMYHI
                     CLKELGISSKECLVFEDSYFGAQASAKANIQTVVIWDALIDKKSYPEEVIGFIPDFEG
                     LAGNLDKNLEEVLLDQVKEVLDQPAQEE"
     gene            1205..1558
                     /gene="metG"
                     /locus_tag="PLF29_02345"
     CDS             1205..1558
                     /gene="metG"
                     /locus_tag="PLF29_02345"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HXK52801.1"
                     /translation="MTPITKQKPEIDFEDFSKVEIRVGEIIECDYLEGSEKILKLKVD
                     FGDLGLRQILSGIAKHYSPQELLKKKTLFVLNIKPRKILGYESQGMLFAADNQEGRPV
                     FVVPEQNLHNGAEMI"
     gene            1568..2752
                     /gene="miaB"
                     /locus_tag="PLF29_02350"
     CDS             1568..2752
                     /gene="miaB"
                     /locus_tag="PLF29_02350"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HXK52802.1"
                     /translation="MQKTYSQKKYFIKTYGCQANVADSGTMAGMLEAVGFEKTDSLEN
                     ADVFIVNTCSVRQKSEDKVYGLGKDIKTLHDKGYKKPFTIISGCMVGSVTGTRQRYEF
                     EELRKKTSWADAYINPSQLSELPVILLGHKVLDDWAVSKINNDYTFGKQSNKTQAFVN
                     ISYGCDNFCTFCVVPYARGKEISRSESEILKEIKHLTQRGYKQVMLCGQNVNSWGLDA
                     TEKFSIRTGSNQKLPFAELLRKVAALEEIEKIDFISSNPFDFTQDLIDVLKTPKISNY
                     LHIAVQSGNNEVLKKMNRRHTIEEFYTLIEKIKLVKPNIELGTDIIVGFPGETEEQFE
                     DTVKLFEKITFNVAFISIYSPRKGTPAEKFYKDDIPLKEKRRRHERLTKIWKSSKVRK
                     EK"
     gene            2719..3684
                     /locus_tag="PLF29_02355"
     CDS             2719..3684
                     /locus_tag="PLF29_02355"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF013845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HXK52803.1"
                     /translation="MEKFKSQKREIVAIVGPTSSGKTELAIKLCKKYDGEIISADSRQ
                     IYEYMDIGTNKTPQNRDYKKYHGFWEIDNIRVWGYDLITPDKEYSAFKYVRYVNSILP
                     NINLKKKIFIVGGSGFYLDCLIGKIRLERAGKSGGLRKDLNKKTTNELLQILKDLRFD
                     QPANFDINNRVRLIRAIEKLQSPILNKEGKATKKSTDFITGNITTKSIIGLTAPREYL
                     YDRADAWVDYIWGDNFYSELQGLISRGYDNYPAFQGLIYKQGLNYYKGLNSLEETLSL
                     SKFAVHAYIRRQQTWFKRNKDIIWFDLSNGSKLSSNMIKIENLLK"
     gene            3689..4675
                     /locus_tag="PLF29_02360"
     CDS             3689..4675
                     /locus_tag="PLF29_02360"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HXK52804.1"
                     /translation="MDRQIIISIKTILLVAAGIATTFIVYQMRGIFLILLIALLLVIS
                     LEHAVLFFQKQTVLNKPVPRSLAVIMAYLSFVLIILLAVTVVMPPVLIQTQILISKIS
                     GLSFQVQGTEFKFQNLVPDSATLSSQLFNRSRSLFTNLANIMSVLILALYISLDWENM
                     KRRGISLLNDPYKTLVKNIISEIEVSIGQWVKGQAILMLTIGSISTVGLLILDVDYAI
                     PLGIAAGMLEVVPLLGPIVATVLAGIVGFTDSYTKGLAVTAMFIIVQQLENNLLVPKI
                     MHKVSGFSPLVILMAMLIGTDLLGVVGALLAIPMMMIGVIVFRRVAKYPVKS"
     gene            5072..6148
                     /gene="mltG"
                     /locus_tag="PLF29_02365"
     CDS             5072..6148
                     /gene="mltG"
                     /locus_tag="PLF29_02365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HXK52805.1"
                     /translation="MNTDLDPSKYHVISVQKLKKIVFFSLIGIAIIGPLVSYGYYKFA
                     VTRPSQTDKEVTYRVEKGQSVADISNELYSAEAINSAFLLKLYIVINKLQDNIQAGNY
                     IIPAGSSVAEVANLLQHGKDDVDITFTEGMRVEEYALAASKTFKNIGYENFIELAKSY
                     EGTLFPETYRFNIEVKENEIIDQMRETYKQRTASILTQENILKTGLSEKEILILASIV
                     EREASDPAERPVVAGILINRFKEGELIGADATTQYAIAAKRYGCDTNSPVVCPNKTQA
                     EEVNWWPHDLTVDELKDQSPYNTRAIVGLPPAPICSPGLDAIEAVINYTQTDYNYYLN
                     DSQGKTHYAKTLEEHNINVSKYLR"
     gene            complement(6140..>6628)
                     /locus_tag="PLF29_02370"
     CDS             complement(6140..>6628)
                     /locus_tag="PLF29_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52806.1"
                     /translation="ESYTRGLVLLEAMKKEPAASPTIWDTVKRVLCYYNIHLLHFSIS
                     EIVDNALYSYVVLSCSGQSCNSLKTFFKPGSTDVAGSGSELSSFVVPGPAFADKGGPE
                     DLYKETHASASETAEILIDLVDGLCLSYQLNVPILLSPKVVEEAGIKITKELLESAIN
                     SS"
BASE COUNT         2246 a         1290 c         1318 g         1774 t
ORIGIN      
        1 gagccgcact tcatacattc gaaattttca cccggcttca aacaagcagg gcaaatgtat
       61 aaaccgtcct tatatattaa ctcggacctt tcgcatctat tacataaaaa aaaaacatcg
      121 tcctgtgaaa catctccaca acttggacat gtgaattttg ctttactttc agtatatata
      181 gtgtcgtcgt gtaaatccat aaaaaaatag taacatactt taaacattta taacaacgtg
      241 ctaatctaaa aatatgttac agcttcttct acttttattg ctggggatat acttagcgat
      301 tggtctcata tacgctatat acctattact cttcacaacc gagctttggt ataagtttcc
      361 actaaacgtc attataggtc ctataggttt ggtcttaaac attaaaagcg cactaaaaat
      421 tagacgaaag tcaatataaa tgtacaaaag cctactacaa aataaaaaag ccgttatatt
      481 tgatcttgac ggcacaataa cagacactga gtatttttgg gccaaagcca tagttaacat
      541 agctgatgaa aattatggct ttgtcgaata taacatcgcc gaaacggccg gcttagagct
      601 tacggacaga tgggagcata ttttactaaa aagacagata gagccaaaga ttccgtactc
      661 tgatttggtg aaaatgacta taacaaggtt tgtgcaatac ataaccgaag cggaaactat
      721 aaaaacctta cccggcttct ggagcctagc tacagaatta aaactggaaa aaaatatcaa
      781 aataggtctt acaacaaaca ctagaaaaga gctagttaat acaatacttg ataaaatcgg
      841 tctttctgag ttttttgatg ccgttgtaac cttagatgat gctaaaaaac ctaaaccgga
      901 tcccgaaatg taccatattt gtcttaagga acttggcatt tcttccaagg aatgcttggt
      961 ttttgaagac tcatactttg gggcccaagc gtcggctaaa gcaaatattc aaacggtagt
     1021 gatttgggac gctttgatag ataaaaaatc ttatcctgag gaggttattg gatttattcc
     1081 ggactttgaa ggtttagcag gcaaccttga taaaaatctg gaagaggttc ttctggatca
     1141 agtgaaagag gtgctagacc aaccagcaca agaggagtaa ttcaaaagat ttataaaggc
     1201 aaacatgacc ccgataacaa aacagaaacc tgaaatagac tttgaagatt tttcgaaggt
     1261 agaaataaga gttggagaaa ttatcgaatg tgactatctt gaaggatcag aaaaaatcct
     1321 taagctaaag gtagattttg gggatcttgg tctacgacag atactaagcg gcattgccaa
     1381 acattactcc ccccaagaat tattaaagaa aaaaacctta tttgtgctaa atataaaacc
     1441 tcgtaaaata ctgggctacg agtctcaagg aatgcttttt gctgccgata atcaggaagg
     1501 caggcccgtt tttgttgtac ccgagcaaaa tctgcacaac ggcgctgaga tgatataatc
     1561 cagatcaatg caaaaaacct actcacaaaa aaaatatttt attaaaacat acggctgtca
     1621 ggcaaatgtc gccgattcgg gaaccatggc gggaatgctt gaagctgtag gttttgaaaa
     1681 aaccgactca cttgagaatg ccgacgtttt tattgtaaat acctgctctg taagacaaaa
     1741 aagcgaagat aaagtgtatg ggttgggaaa agacattaaa actcttcatg ataaagggta
     1801 caagaagcct tttacaataa tctcagggtg tatggtcgga tcggttacag gaacaagaca
     1861 gaggtacgaa ttcgaagaat taagaaaaaa aaccagctgg gcagatgcat atataaaccc
     1921 ctcccaactt tcagagttac ccgtgatcct gctagggcac aaggttctgg atgattgggc
     1981 tgtttcaaaa ataaataatg attacacctt cggcaaacaa tcaaataaaa ctcaggcctt
     2041 tgttaacatt tcttatgggt gtgataattt ctgtactttt tgtgtagtac cttatgcccg
     2101 aggaaaagaa atatcaagat cggaaagtga aatattgaaa gagattaaac accttaccca
     2161 aagaggctac aaacaggtca tgctttgcgg acaaaacgtt aattcatggg gactggacgc
     2221 aactgaaaaa ttttctatcc gaacaggaag caaccaaaaa ctgccgtttg ctgaacttct
     2281 aagaaaagtt gcggcactgg aagagatcga aaagatcgat tttatctcat ccaacccatt
     2341 tgattttacg caagatttaa ttgacgtatt aaaaactcca aagatctcaa actatctgca
     2401 catcgctgta caatcaggaa ataacgaggt gcttaaaaaa atgaaccgaa gacacactat
     2461 agaagagttt tatacgctaa ttgaaaagat aaaactggtg aaaccaaaca ttgagcttgg
     2521 cactgacatt attgtcggct ttcccggtga aacagaggag caatttgaag acacagtaaa
     2581 acttttcgaa aaaataacct ttaacgttgc ctttatttct atctactcac cgagaaaagg
     2641 tacacctgca gaaaagtttt ataaggacga cattccgcta aaagaaaaac gtcgtcgcca
     2701 cgaaaggctt acaaagatat ggaaaagttc aaaagtcaga aaagagaaat agtagctatt
     2761 gtaggtccca cctcatcggg taagactgag cttgcaataa aactatgcaa aaaatacgac
     2821 ggggagataa tttcggcaga ttccagacag atatatgagt atatggatat tggcacaaat
     2881 aaaacaccgc aaaatcgtga ttacaaaaaa taccacggat tttgggaaat agacaatatc
     2941 agagtatggg gctacgacct aataaccccc gacaaggaat actcagcgtt taaatatgta
     3001 agatacgtaa attctatatt gccaaatatt aatcttaaga aaaaaatttt catagttggc
     3061 ggcagtggat tctacctaga ctgcctgatt ggaaaaattc gcctagaaag agctggaaag
     3121 agtggaggcc tgagaaaaga cctaaacaag aaaacaacaa acgagctgtt gcaaattctt
     3181 aaagatctgc gttttgatca acccgcgaac tttgatataa acaacagagt tcgacttatc
     3241 agagcaattg aaaaattaca aagtccaatt ttaaacaaag agggaaaggc tacgaaaaaa
     3301 agcactgact ttattacagg aaacattact acaaaaagca taatagggct cacggcccct
     3361 cgtgaatacc tctacgacag agcagatgct tgggtcgact acatttgggg ggataatttt
     3421 tattctgagc ttcagggcct gattagcaga gggtatgata attacccagc ctttcagggc
     3481 ctaatatata aacaaggact aaactactat aaggggctta attcattgga agagactttg
     3541 agtttatcaa aatttgccgt gcatgcttat attagaaggc agcaaacttg gtttaaaaga
     3601 aataaagata taatttggtt cgacttgagc aacggctcga agctatcttc gaatatgatt
     3661 aagatcgaaa atttgctaaa ataattgtgt ggatagacaa attatcatct caataaaaac
     3721 catacttctt gtagctgcgg gtatagcaac aacgttcatt gtttatcaaa tgagagggat
     3781 atttttaatc ctactcattg ccctgcttct tgtcatatct cttgaacatg cggttttatt
     3841 ttttcaaaaa caaactgtgc tgaataaacc cgttcccaga agcttggccg tgataatggc
     3901 ctatttaagc tttgtactaa taattttgct ggcggttaca gttgtaatgc cgcctgtgtt
     3961 aattcaaact caaatcttga tttcaaaaat atcgggacta agttttcagg tacaaggaac
     4021 ggaatttaaa tttcaaaacc tagttcctga ctcagcaaca ctctcctctc aactttttaa
     4081 caggtcgcgc tccttattta ccaatctcgc aaatattatg agcgttctta tcttggcact
     4141 atacatttcc ttagactggg aaaacatgaa acgaagaggc atctcgttac ttaacgaccc
     4201 gtataaaact ttagtcaaga atattatatc tgaaattgag gtaagcatag ggcagtgggt
     4261 taagggtcag gccatactaa tgttaacgat cgggtcaata agtacagtcg gcctgttgat
     4321 attggacgtt gattatgcga tacctctggg aattgctgca ggcatgctag aggtcgtacc
     4381 tttgttggga ccgattgttg caacggtact agccggaatt gtcggcttca cagacagcta
     4441 tactaaaggc ctggctgtca cagccatgtt tataatagta cagcagcttg aaaacaacct
     4501 tctagtgccc aaaataatgc acaaagtctc cggtttcagt ccattggtca ttctaatggc
     4561 aatgcttata ggaactgact tgctgggggt tgtgggggct ttactggcca taccaatgat
     4621 gatgataggg gtaattgtat tccgaagagt agcaaaatat cccgttaagt cctagaagga
     4681 ggctttcgca gaaagcccgt aagccggatt ctgtattatg tagccatcta tctacgcccc
     4741 atctttatac ctcgccgagt aagctatagc ggtaaattcg tgggttgctg caggtgggat
     4801 tgcccgtttc aacttaaacc ttatgcaagc acttaggctc aagctcgtct ctgttgctct
     4861 caaagaatgt cgattcactc tctgttctat taacttttca attaacagac accagcgctt
     4921 ttcccgcaag cgggattata cgtcggggct tgcgccccgt cccctactct gtgcagtccg
     4981 gactttcctc ctttgttaaa aggcgactac taggctttct gcatcgatta ttgtaccaaa
     5041 ttttggcgca atatgttaac ataactctgt tatgaacaca gatctggatc catctaagta
     5101 ccatgttatt agcgtccaaa aacttaagaa aatagttttt ttcagcctca tcggcatagc
     5161 tattatcggt ccgcttgttt cctacggata ctataagttt gcagttacca ggccttctca
     5221 aaccgacaag gaagttacct acagggttga aaagggtcaa agtgtcgcag atatctcaaa
     5281 cgaactgtac agtgccgaag ctataaattc tgctttcctg ctaaagctat atatcgtaat
     5341 aaataaactt caagacaata ttcaagccgg aaactatata atacctgccg gaagctcggt
     5401 agctgaagtc gctaaccttc ttcagcacgg gaaagatgac gtagatataa ctttcaccga
     5461 aggtatgagg gtagaggaat acgctcttgc tgcaagcaaa acatttaaaa atataggata
     5521 cgaaaacttt atcgaacttg ctaaatcata cgaaggaact cttttcccgg agacatacag
     5581 atttaatata gaggtgaaag aaaatgaaat tatagatcaa atgagagaaa cttataaaca
     5641 acgcacagct tctatcttaa ctcaagaaaa catattaaaa acgggacttt cagaaaaaga
     5701 gatattaatt ttagcatcaa ttgttgagag agaggcatct gatcctgctg aaaggcccgt
     5761 agttgcggga attctaataa acagattcaa agagggggaa cttataggag cagacgctac
     5821 aacccagtat gcaattgcgg caaaaagata tggatgtgat acaaactctc cggtggtgtg
     5881 ccctaataaa acccaggcag aagaggtgaa ctggtggcca cacgatctta ccgtagacga
     5941 gcttaaagac caaagcccct acaatactag ggcgatcgta gggcttccgc ctgcaccaat
     6001 ttgcagcccc ggccttgatg caatagaagc agtaattaac tacacacaaa ccgactataa
     6061 ttactatctc aatgattcac aaggaaaaac tcattacgcc aaaaccttag aggagcataa
     6121 tataaacgta tctaaatacc tacgatgaat taatcgctga ttccaacaat tctttggtaa
     6181 ttttaatacc tgcttcttcc acaaccttag ggcttaataa tataggcaca tttagctgat
     6241 aagataaaca aagcccgtca acaagatcaa tcaaaatctc ggcagtttca gaggcagagg
     6301 catgtgtctc tttatacaga tcttcgggcc cgcctttgtc tgcaaaggcg ggccccggaa
     6361 caacaaaaga agacaattcc gagccactac ccgcaacatc ggtgctgcca ggcttgaaaa
     6421 atgttttcaa gctgttacaa gattgccccg aacacgataa caccacatag gaataaaggg
     6481 cgttgtctac aatttctgag atggaaaagt gaagtaaatg gatattataa tagcaaagaa
     6541 cgcgttttac agtgtcccag atagttgggg atgctgcggg ttcttttttc attgcctcaa
     6601 gcaaaactaa accacgagta taagattc
//
LOCUS       DAOSWN010000037         6025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_8666_length_6025_cov_3.839531, whole genome shotgun sequence.
ACCESSION   DAOSWN010000037 DAOSWN010000000
VERSION     DAOSWN010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6025
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8666_length_6025_cov_3.839531"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PLF29_02375"
     CDS             <1..217
                     /locus_tag="PLF29_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="macrolide ABC transporter permease"
                     /protein_id="HXK52807.1"
                     /translation="EAVTLSVFGGLIGLFLGYLTSLAVQSFVRAEITPIAIILAFLFS
                     VVVGVIFGTYPAVQASKKDPIEALRYE"
     gene            210..1400
                     /locus_tag="PLF29_02380"
     CDS             210..1400
                     /locus_tag="PLF29_02380"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXK52808.1"
                     /translation="MSSKRLVEALKTSITALKANKIRTFLTTLGVIIGVFSVVSLVSL
                     GKGAQNYIEDQFNAIGSNLIFVSPGKVDLSDDPAKFFSKNKLAEKHVKLIENALGDKV
                     VAVTPSIRVGQTVQYKTKSFYPTVIGANEQLLLAFDFNVDQGRAFNSSAVSSKSRVAV
                     LGKNVAQELFAGIDPIDKKIKIGKDTFKVVAVLSEKNRNFDDNVYIPYTTAKEVFDVR
                     NFSSIGIKVKDSTQVKAVMREVELTLLKDLKKDDFSVLSQQDILSTIQNVLNILTLGI
                     GAIAGISLVVGGIGIMNIMLVSVTERIREIGLRKALGATSFDIGLQFVIESILLSIIG
                     GLVGLAMGAAVALAARSAVRTEVPFSTVVLAFTFSLVVGVVFGTYPALQASKKDPIEA
                     LRYE"
     gene            complement(1426..1962)
                     /locus_tag="PLF29_02385"
     CDS             complement(1426..1962)
                     /locus_tag="PLF29_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52809.1"
                     /translation="MRQNILKITPLLVLLLVIFAGRSYVLNVLKDDSVEIVQKMDEKK
                     VPNVAPANIMITVEGERISIKKSLRLSNTDTFIEAMESLREEGYISFERLAYIDGMRI
                     TAINNIESNDINEWRVFLSMKEGQSTSADPLLFDDYELNILKQAEGAEVDITQYIDTM
                     NIIDEGDYRIVYIKISDM"
     gene            2008..2084
                     /locus_tag="PLF29_02390"
     tRNA            2008..2084
                     /locus_tag="PLF29_02390"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2042..2044,aa:Arg,seq:tcg)
     gene            2102..2404
                     /locus_tag="PLF29_02395"
     CDS             2102..2404
                     /locus_tag="PLF29_02395"
                     /inference="COORDINATES: protein motif:HMM:NF013869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HXK52810.1"
                     /translation="MKGFIEFIREQGVVGLSVGFILGGAVSKVVASLVNDVINPVLGL
                     IMGAAGGLQNAQLMIGGAEIHIGNFISAIIDFLIIGAIVYYGIRGLKLDNLDKKKS"
     gene            2492..2566
                     /locus_tag="PLF29_02400"
     tRNA            2492..2566
                     /locus_tag="PLF29_02400"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2524..2526,aa:Lys,seq:ctt)
     gene            3208..3669
                     /locus_tag="PLF29_02405"
     CDS             3208..3669
                     /locus_tag="PLF29_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52811.1"
                     /translation="MRNINLVDRATAEWNVDYIHSRLVTAIIGDNDMCRSLRKPENMV
                     MRRVAEALFTHRDGHVDSVVDGINYIIETNETPGLALIAYLDKAAEENLPREKIFFHL
                     LGSAAALTLLGKPEHAAFAFRLDPDPEDPHGLKDFPFLPEIKKAFFKCPRS"
     gene            3602..4072
                     /locus_tag="PLF29_02410"
     CDS             3602..4072
                     /locus_tag="PLF29_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52812.1"
                     /translation="MVLRIFPFFPKLKRLFLSALGPEVIANFRDQIEGNQAQFAQGCS
                     EACGAFLGQPVGELSPDLINTIDQVCKTQGWVIGKLSKDDVAEALLKTRRDKDSRSSI
                     LDRVKSDPNPLTIIEAYGELSRLKDEISILSSQTRLATFRLSENDYLASKNSLN"
     gene            4105..5595
                     /gene="crtI"
                     /locus_tag="PLF29_02415"
     CDS             4105..5595
                     /gene="crtI"
                     /locus_tag="PLF29_02415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HXK52813.1"
                     /translation="MNKTAAVIGSGFGGLSIALRLLSEGYKVTIFEKNSSVGGHASQY
                     KEKGYTFDMGPSIITLPFLIDELFQLFGEDRKSYLDLQALDPYYRIYFHDKTYIDYSG
                     DENQMVEQLTRFNTKDGKAYYSFMRYSKNLYKSVILDGLGSKPFLSMFDFIGYFPKAL
                     ITGAILPSYWVVARFFKDFRARFLFSFHALFIGGNPFITPSVFLMLPYLEKDGGVWFT
                     KGGMYSVVEALSELFKKNGGVIKTNTEVTEIVVEDDAAKGVRVGPEFYSFDIVVSNSH
                     FANTYLDLLKGRSRGKWKSSKIKKMHYSMSCVVLYLGIKKQYPQLLHHTLIISKRYKG
                     LIQDIFKSKVLPDDFSMYLHAPSRTDKSMAPEGCESLYLLVPVPNLAANIDWDVEAEP
                     FSNKIIDFLENSFGLENLRNNIEVQKVFTPLNFRTERNNYLGACWSLQPRYDQIASLR
                     PHNKAENIKNLYFVGASVHPGGGVPGVILSAKATHKIILNDYGDKK"
     gene            5605..>6025
                     /locus_tag="PLF29_02420"
     CDS             5605..>6025
                     /locus_tag="PLF29_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HXK52814.1"
                     /translation="MKSSVSKRIQRKFLQAFSIGSKTFYFSALFLPKQYRVEIANLYH
                     FLRQIDDIADMAKGNNPEAIIKIAKSKDLKDFLDRSKIDSHYFEELVSGILSDTDFKP
                     FNEFNDVCTYCYKVAGTVGIMICNIFGVTDKKTYDYAA"
BASE COUNT         1835 a         1084 c         1232 g         1874 t
ORIGIN      
        1 agaagcagta actctcagtg tttttggagg actgattggg ttgtttctcg gttacttgac
       61 ttctcttgca gttcaaagct tcgtgagagc agaaattact cctatagcaa taattctagc
      121 gttcttgttt tcagtagttg taggtgtaat atttggtaca tatcctgcgg ttcaagcaag
      181 taaaaaggac ccaattgagg cattaagata tgagtagcaa aaggctcgta gaagcattaa
      241 aaacatctat tacagcacta aaagctaata aaatacgtac ttttttaacc accctgggtg
      301 taataatcgg tgttttttcg gttgtatcgc tggtttcttt aggaaaaggt gcgcaaaact
      361 acatagaaga ccaatttaat gctatcggtt ctaatttaat atttgtatca ccggggaaag
      421 tggatctctc ggatgacccg gcaaaatttt ttagtaagaa caagctggca gaaaaacatg
      481 taaagctcat tgaaaacgct ttgggagata aggttgttgc agtaacgcct tcaattcgag
      541 taggtcaaac ggttcagtat aaaacaaagt ctttttaccc taccgttatt ggtgcgaacg
      601 aacagctttt attggctttt gatttcaacg ttgatcaagg cagagcattt aattcttctg
      661 ctgtaagctc taagtctcgg gttgcagtgt tgggaaaaaa tgttgcccag gaactttttg
      721 ccggaataga tccaatagat aaaaaaatta aaatcggaaa agatactttt aaggtcgtgg
      781 cggtgttatc tgaaaaaaat agaaattttg acgataatgt ctatattcca tatacaaccg
      841 cgaaggaagt ttttgatgtt agaaattttt caagcattgg aataaaagtt aaagattcta
      901 ctcaagttaa agctgtcatg agagaggtag agctcactct cctcaaagat ttaaaaaagg
      961 atgatttctc tgtcttgtct cagcaagaca tattaagcac tattcaaaat gtattaaata
     1021 ttttaacttt aggaataggc gctattgcgg gaatatcttt ggttgtgggg ggaattggaa
     1081 ttatgaatat aatgcttgtg tccgtaactg agagaattcg tgaaataggg cttagaaagg
     1141 ctcttggagc tacatctttt gatattggtc ttcagtttgt tattgaatct atcttgttaa
     1201 gtataatcgg aggtttggtg ggcttagcaa tgggggcagc tgtagcatta gccgccagaa
     1261 gcgcagtacg tacagaggta ccattttcaa cagttgtgtt ggcattcacg ttctctcttg
     1321 ttgtcggtgt tgtctttgga acttatcctg cgctacaggc cagcaaaaag gatcctattg
     1381 aagcattaag gtacgaataa gaatttttag agcaggttcg cccttttaca tgtccgagat
     1441 ctttatatag acaattctat agtcaccctc atctatgata ttcattgtat ctatatactg
     1501 tgtaatatct acctctgcgc cctctgcctg ctttaagatg tttaactcat agtcatcaaa
     1561 taataatggg tctgcagaag tcgactgccc ctccttcatg cttagaaata cacgccattc
     1621 atttatgtcg ttactctcta tattatttat ggctgttatt ctcatcccgt caatataggc
     1681 caaacgttca aaacttatat agccttcctc tctcagagac tccatagcct caataaaagt
     1741 gtctgtgttc gataacctta gacttttctt tatagatatt ctttcgccct ctactgtaat
     1801 cataatatta gcaggcgcaa cgttcggaac ttttttttca tccatttttt gaactatctc
     1861 tacactgtca tctttcaaga catttagaac ataagatcta ccagcaaaaa ttactaataa
     1921 aagcacaagg agtggggtaa tttttaaaat attttgtctc actcctatat tttagcacta
     1981 gatgttataa tcaaaaatag caaatacgcc tctgtagctc agcggatcag agcactcggc
     2041 ttcggaccga ggggtcgggg gttcgaatcc ctccaggggc gccataattg aaggttgaaa
     2101 tatgaaaggt tttattgaat ttattagaga gcaaggtgtc gttggcctat cagtaggatt
     2161 tatactagga ggcgcagtct ctaaagttgt tgcctcatta gttaacgatg taataaaccc
     2221 ggtgcttgga ctcattatgg gagccgcggg cggtctacaa aacgcacaat taatgattgg
     2281 cggtgcggag attcatatag gtaactttat ttccgctata attgactttt taataattgg
     2341 ggctattgta tactacggaa taagaggctt aaagctcgat aatctcgaca aaaagaagtc
     2401 ctaatttacc tgcgtacatt taaatttacc attatttctg ctattattta ccgtgatgtg
     2461 tgttgtaagc agttcgcgca tctttttgtt cgggagcgta gctcaatggc agagcaaccg
     2521 gctcttaacc ggtaggttgt gggttcgaat cccaccgctc tcaccactat ttgatactta
     2581 aatagcaaac gtgttaaaga ctatcttgag atacagagtt taaaatcaaa aaatatatta
     2641 gtttgttcgc tagtagtaca cttcgggaag aggataacgt ctttgttatt cgataattca
     2701 aaatatgttg ttgctgttat tactgaattg aactcaaggg ccgttaaagc tctggccgga
     2761 gaagtatgat acctgctcta ttattatcaa actgaagaaa aaaatgtggc cgctgctgtt
     2821 ggatttattt gtggggcagt ttatttaggc cggattagat gtgcccttac tgttttgaca
     2881 agccttgggg aacatgctgg gaaagaccaa agggtacttg ggtatgcccc tgaagaacta
     2941 tttgataaag ttaaaaatgc ctcagatatg ctacaaaaaa atcataaaat actatctgca
     3001 ataaggctat aatattcgca atatagacta tggtttatag ggtttaagca agcaatttca
     3061 acggtaagga aatggttgac ggcctataac cttacgccat ataatgatag ctgtaaaagg
     3121 gcctactaca tatttcattc ttttactaat tttctggtct ttttggacta gcaaatatca
     3181 ggaggcccgg atcaggctca attttatatg agaaatatta atttagtaga cagagctaca
     3241 gcagaatgga atgttgatta tattcatagc aggcttgtta ccgcaataat aggtgataat
     3301 gatatgtgta ggtcattacg aaagcctgaa aatatggtta tgcgtcgagt agctgaagca
     3361 ctttttacac atcgagatgg gcatgttgat tctgtggttg atggtataaa ctatataata
     3421 gagactaacg aaactccagg cctcgcgcta atagcttacc tcgacaaagc tgcagaagaa
     3481 aatcttcctc gtgaaaaaat atttttccat cttctaggtt cggctgcagc cctcaccttg
     3541 cttggaaaac ctgaacatgc tgcattcgct tttagactcg acccagatcc agaagatcct
     3601 catggtctta aggattttcc ctttcttccc gaaattaaaa aggctttttt taagtgccct
     3661 cggtcctgaa gttattgcaa attttagaga tcaaatagaa gggaaccaag ctcaatttgc
     3721 acaaggctgt tcagaagcct gcggtgcatt tctaggccaa ccggtgggcg aattatcccc
     3781 cgatctcata aatactatag accaggtttg taaaactcag gggtgggtta tcgggaagct
     3841 ttccaaagac gacgttgctg aagcacttct aaagaccagg cgtgataaag atagtcgatc
     3901 tagtatttta gatcgtgtta aatcagaccc caatccgtta actattatag aagcttacgg
     3961 cgaactttct cgcctcaagg atgaaatctc catcctttcc tcccagacta gacttgctac
     4021 atttagacta agcgaaaatg attatctagc aagcaaaaat tctctaaact aacacaccct
     4081 gaatatttta ggtataatat ttccatgaat aaaacggctg ctgttatagg ttctggtttt
     4141 ggaggacttt ccatagcgct caggctctta tccgaaggtt ataaggtcac gatttttgaa
     4201 aaaaattctt ctgttggggg ccatgccagt caatacaaag aaaaaggata cacgttcgat
     4261 atgggccctt ccattattac gctacctttc cttattgatg aactgtttca actctttggc
     4321 gaagaccgta agtcttattt agatctacaa gctttagacc catattatcg aatttacttt
     4381 cacgataaga cctacattga ctattccgga gatgagaacc aaatggttga acaacttaca
     4441 agattcaata cgaaagatgg aaaggcatac tatagcttta tgcgttattc aaaaaatttg
     4501 tataaatcgg taatcctaga cggactgggc tctaagcctt ttttaagcat gttcgatttt
     4561 ataggttatt ttccaaaagc actaataacc ggggcaattc ttccttctta ttgggttgtg
     4621 gcaaggttct tcaaggactt tagagcaaga ttcttgttct cattccacgc attatttatt
     4681 ggtggaaacc cctttattac accatcggtg tttttaatgc ttccttactt ggaaaaggat
     4741 gggggagtgt ggtttacaaa aggaggcatg tatagcgttg tggaggctct gagcgaactt
     4801 tttaaaaaaa acgggggtgt tattaaaaca aacacagaag ttacagaaat agttgtagag
     4861 gacgatgccg caaaaggtgt aagggttgga ccagagtttt atagttttga cattgttgta
     4921 tctaactcac actttgcaaa cacctattta gatttactta aaggaagaag cagaggaaag
     4981 tggaaatctt caaaaataaa gaaaatgcac tatagcatga gctgtgttgt tttatacctg
     5041 ggaattaaaa agcaataccc ccagttgttg catcacacac ttattatttc aaaacgatac
     5101 aaaggattga ttcaagacat ctttaaaagc aaggttttac ctgatgattt ttctatgtat
     5161 ctgcatgcgc caagtagaac cgataagtct atggctcctg agggttgcga gagcctgtat
     5221 ctgcttgttc ctgtcccgaa tcttgctgca aatattgact gggatgtcga agctgagcct
     5281 ttctcaaata aaattataga ctttctcgaa aattcttttg gattggagaa tttgagaaat
     5341 aatattgagg ttcaaaaagt ttttactccg ctaaatttca ggactgagcg caataactat
     5401 cttggggcat gctggagctt acagcccagg tacgatcaaa tagcctctct caggccccat
     5461 aacaaggcag aaaatattaa aaatttatat ttcgttggcg caagcgttca tccgggcgga
     5521 ggtgtgcccg gagttatttt atcggcaaaa gctacccata aaataatatt aaatgactat
     5581 ggcgataaaa agtaatccaa tacaatgaaa agttcggttt ctaaaagaat tcaaagaaag
     5641 tttttgcagg cttttagcat aggctcaaaa acattttact tttctgccct gtttctgcca
     5701 aaacaatata gagttgaaat agctaatctg taccattttc ttaggcaaat tgatgacatt
     5761 gccgacatgg caaagggcaa caatcccgag gcaataatta aaatagcaaa atcaaaagat
     5821 ctcaaagatt ttttggatcg tagcaaaatc gatagccatt attttgaaga gcttgtttca
     5881 ggaattctta gtgacacaga ctttaagcct tttaatgagt ttaacgacgt ctgcacatac
     5941 tgctataaag ttgcaggaac tgtaggcatt atgatatgta atatttttgg ggttaccgat
     6001 aaaaaaactt atgattatgc agcta
//
LOCUS       DAOSWN010000038         5999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_8720_length_5999_cov_4.503028, whole genome shotgun sequence.
ACCESSION   DAOSWN010000038 DAOSWN010000000
VERSION     DAOSWN010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5999
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8720_length_5999_cov_4.503028"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="PLF29_02425"
     CDS             complement(<1..281)
                     /locus_tag="PLF29_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52815.1"
                     /translation="MPHHEYVTSLLYKKKTPLGFSLALSASLGIINSIFVLGWILDIK
                     WLIDPFTSESPTKLIAAISFVCVSIIILSLSYDNNARPPLLTLIHIAVT"
     gene            complement(506..1819)
                     /locus_tag="PLF29_02430"
     CDS             complement(506..1819)
                     /locus_tag="PLF29_02430"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HXK52816.1"
                     /translation="MNTKKYLLIAASLVLSSTILTSSTYAREVEPGNGDVNKDGTTNV
                     LDALLIRQYLDGIKNISLAQKWAGDVDGNGELSTNDADMIMQRDIGIITTLPYEEDDF
                     AYNNVLFGDTDGNGDVNSVDALKIEQYAQNMISLNLLQLYISDVNASDSVNTEDANLI
                     LQEEVGLISCFPVMPDCDEDSSSSTAPEDTQTTTNTSSPTSKDKDKDKDNDNKDNKDE
                     KDSDKNEASTNNEGSQNLNTQASPSVAQTVTLDESLIVNPQDKAKTIKLFGKTIVPNT
                     RFMLTIYSDPIRNELTSDENGNWEYELAPDTLPAGEHRITVAYFNWDDSMSEETEIAK
                     IEVVNNDSAINTKIAEAQRQLSELNESTNAQMGVLKLLLLALATMLILVLHRINKKHL
                     LILSNQNTGNAPEVQNGTKEMATKIEETVVKKSKKASKNTTKEKK"
     gene            complement(2056..2682)
                     /locus_tag="PLF29_02435"
     CDS             complement(2056..2682)
                     /locus_tag="PLF29_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52817.1"
                     /translation="MKDLTKREERKKYENGNLLGGAYNFALTSFLFIGAMYFIYYSLS
                     EAKVYYLLSPLLIYFSGKLIRKKYASRTIQRKYIGEFAILLFSYLCLAYFLYVLGIDA
                     KIINLLPKSLFIQSAAAFIGIIILSVIGSSKLYYRFKHIMLFILTILFVALQQYTHEM
                     NLFFESNPQIINYIGGIPILYLISVIFGLSDIYDLHIKQKVTPAHNLE"
     gene            complement(2666..3046)
                     /locus_tag="PLF29_02440"
     CDS             complement(2666..3046)
                     /locus_tag="PLF29_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXK52818.1"
                     /translation="MNNYLDNIQKLTLENKNFRKVLFTGKHTQLVIMSLLPGEEIGLE
                     IHPDTDQFFRIEQGQAKIIMDGQEFFAEDDYAIIITAGTEHNVINASETEVLKMYTLY
                     SPPNHPEGTIHKNKEEADLYERSH"
     gene            complement(3097..3492)
                     /locus_tag="PLF29_02445"
     CDS             complement(3097..3492)
                     /locus_tag="PLF29_02445"
                     /inference="COORDINATES: protein motif:HMM:NF015060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HXK52819.1"
                     /translation="MTSYEWYSTLKKPFFAPPSNVFGPVWGFLYLIIFVSYSYVFYLF
                     FKKSLTVAEVAPFLLNLAFNIAFTPLQFGLKNNYLALVDIILVLITLIWGMFSIYKHS
                     QIVFLAQIPYLLWVLFATALQISITYLNR"
     gene            3657..4277
                     /locus_tag="PLF29_02450"
     CDS             3657..4277
                     /locus_tag="PLF29_02450"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXK52820.1"
                     /translation="MNLKYPVDHTKYIIFQEFGNDNTNNQTRKEFYTVFDNKHPGVDF
                     LTPVGVTVRASFEGIVVRKEFHKGMGNVIGIRNGNIVALYAHLKDFSVELGDIVKQKQ
                     DVGHSGETGSACILPHLHFELRDISKNSLKEMVFKPEFLSEVSLYSEHFYYTVNNTNT
                     QKTLAKLSVLYFGTTKYWMNIAQFNKLSQSQDEDLQNGKKLLIPNY"
     gene            complement(4374..5141)
                     /locus_tag="PLF29_02455"
     CDS             complement(4374..5141)
                     /locus_tag="PLF29_02455"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HXK52821.1"
                     /translation="MLFLDLKESLSKETSKLRGQVGLYVKFLNSEQEYKHNENDQFWA
                     ASTIKVPIALAFLAFTKNNELKLTDRISIEESNYVKGSGILKLIDNGALLTYKDLLTL
                     MLIVSDNTATNQILDIVDPENVEEFIKNVGLKNTTVRHKMMIKAGKGPNLTTPNDMGI
                     LLEKMYKNELFGSEQILKIMQEQLDRTRIPLYLPNNIDISYKNGSLEQAVHEIGLIRS
                     KNPFIFCFYSDDQNDKRATSEVLSRCAKLCFDYSQEC"
     gene            complement(5431..>5999)
                     /locus_tag="PLF29_02460"
     CDS             complement(5431..>5999)
                     /locus_tag="PLF29_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52822.1"
                     /translation="LADKKPPEFVAKGEEKTLPEDLRRLMREALGSRLYEAVTGSETF
                     KIFIELGLEAAQNEGSFPTSAWQMVLGYGIIRTLLLEKDLKEHIKNSETRFFDTLHQV
                     SSLIVSPGLAALSTEERELTRIEARASVMCLEPGVDYINNVNDPYARASLIYYLALHD
                     LSVALSGDNSNSGVSKAVDLLRGPARVI"
BASE COUNT         1746 a         1076 c         1126 g         2051 t
ORIGIN      
        1 atcgtaacgg cgatatgtat aagtgttaaa agaggcgggc gagcgttgtt atcataagaa
       61 agagacaata ttataatgct cacgcacaca aaagatatcg ctgcaattag tttagtagga
      121 gactcgctgg taaaagggtc tatgagccat ttgatgtcta aaatccatcc caaaacaaag
      181 atagagttga taataccaag cgatgcggaa agcgctaagg aaaaacccag aggcgttttt
      241 ttcttatata atagagaagt tacgtattcg tggtggggca ttattaaaat tatagacaac
      301 aagtgtacaa aataaagcaa ttgggaaacg aaaaaaccac cttgcggtgg tttcttcggc
      361 gcccctgcga gaggaactct cactgggaca atgtttaata tacaacactt atactactct
      421 gtcaacattt ttgtagtcta tttttttaag ttgccgaacc ttctgtttag gtcctcatga
      481 aaggcttttc tctctattac ctactttact tcttttcttt agtcgtgttt tttgaagctt
      541 tttttgattt ttttacaacc gtttcttcaa tttttgtggc catctctttt gtcccattct
      601 gtacttccgg ggcgttacca gtattctgat tcgagaggat aagtaggtgc tttttgttta
      661 ttctatgaag aaccagaatt agcattgttg ctagagcaag cagcagaagt tttaatacgc
      721 ccatttgagc attagtgctc tcatttaatt ccgaaagctg tctttgagct tctgctattt
      781 ttgtatttat cgcgctgtca ttattaacaa cttcaatctt tgcgatttcg gtttcctcag
      841 acatgctgtc gtcccaatta aagtatgcta cggtaatcct gtgttccccc gccggaagtg
      901 tgtcaggggc caattcatat tcccagtttc cgttttcgtc actggttagc tcgtttcgta
      961 ttggatcgct gtatattgtc aacataaaac gagtgtttgg aacaattgtt ttaccaaaga
     1021 gttttattgt ttttgcttta tcttgagggt ttacaattaa gctttcatct aaggttactg
     1081 tttgtgcaac actagggctt gcttgagtgt ttaagttttg actcccctcg ttgttggtag
     1141 aagcctcatt tttatcggaa tctttttcat ctttgttatc tttgttgtca ttatccttgt
     1201 ctttatcttt atcttttgat gttgggctag aggtattagt agttgtttgc gtgtcttccg
     1261 gggcagtact tgatgaagag tcctcgtcgc aatccggcat taccggaaag cagcttatta
     1321 atcctacttc ttcctgaaga ataaggtttg catcttccgt attcaccgag tctgatgcgt
     1381 tgacatctga aatgtacagc tgtaaaagat ttagagaaat catattctgc gcatattgct
     1441 caatttttaa tgcgtcaaca gaatttacat ctccgttacc gtcggtgtca ccaaataaaa
     1501 cgttgttgta ggcaaaatca tcttcttcgt atggcaatgt ggttattatt ccaatgtcgc
     1561 gctgcattat catgtcggca tcatttgttg aaagctcacc attaccgtca acatctccgg
     1621 cccatttttg agccagtgag atatttttta ttccgtccaa atactgccta attaagagag
     1681 cgtctagaac attagttgtt ccgtctttgt ttacgtctcc gtttcccggt tcaacctctc
     1741 tcgcataagt tgaactagtt agtatcgtgg aagacagtac gagcgaggcg gctatgagaa
     1801 gatatttttt agtattcatg tgtagattat agcaaaagtg tcaacccaaa gccttgttgc
     1861 agtattactg gttccgattc agcatatcgc gccatttcac ctattctgtt acagagtttc
     1921 tgtttctttg atctactaaa acaatctgtg ctaaattaac caccaccacc ttttacaggc
     1981 ggcttgctat tctttcttta cgtgcaaagc gcaaaatgct aagtaaacta cacctgctag
     2041 cccatgtaat acgacttatt ctagattgtg tgcgggcgtt actttttgct ttatatgtaa
     2101 atcgtaaatg tcgctaagtc cgaatattac tgaaattaaa tatagtattg gaatgccccc
     2161 gatgtagttt ataatctgcg gattactttc aaaaaagagg ttcatttcat gtgtatattg
     2221 ttgtagagcg acaaaaagta tggtaagtat aaagagcatt atatgtttaa acctgtaata
     2281 taactttgaa gatcctatca cggataaaat aataatacct atgaaagctg ctgccgactg
     2341 aataaataaa ctttttggca gtaaatttat tatttttgcg tcaatgccta aaacatataa
     2401 aaaatacgcc aggcacagat atgaaaataa tagaattgca aactctccga tatattttct
     2461 ttgaattgtt ctgcttgcat atttttttct aattaatttg ccggaaaaat aaattaacaa
     2521 cggagaaagt aaataatata cttttgcttc agaaagagaa taatatataa agtacatggc
     2581 ccctataaaa agaaaggaag ttaaagcaaa gttgtaggct ccgcctaata ggtttccatt
     2641 ttcgtatttt ttgcgctctt ctcttttagt gagatctttc ataaagatcg gcctcttctt
     2701 tatttttatg tatggttcct tcaggatgat ttggcggaga gtagagtgtg tacattttca
     2761 atacttccgt ctcggatgca ttgataacat tatgctctgt tcctgcagtt attattatgg
     2821 cgtagtcatc ttctgcgaag aattcttgcc catccataat aattttagct tggccttgtt
     2881 caattctgaa aaactgatct gtatccggat gtatctctag tccaatttcc tcacctggca
     2941 aaagagacat tattacaagc tgcgtgtgtt ttccagtgaa aaggaccttt ctaaagtttt
     3001 tgttctctaa ggttagtttt tggatattgt ctaaataatt attcatacac taagtatagc
     3061 atcgtatagg attttttgtg aaagttctct tagcatttat ctattaagat aggttatcga
     3121 aatctgtaga gctgttgcaa ataaaaccca aagcagataa ggaatttgtg ctaaaaaaac
     3181 aatctgagaa tgcttgtaga tagaaaacat cccccatatt aatgttatca gcacaagaat
     3241 aatatccact aaagccagat aattattttt taaaccaaac tgcagcggcg taaaggctat
     3301 attaaatgcc aagttaagca aaaacggagc tacttcggca acagttaaag attttttaaa
     3361 gaaaagatag aaaacataac tataacttac gaatataata agatatagaa agccccagac
     3421 tggcccaaaa acgttgctgg gtggggcgaa aaaaggcttt tttaaagtag agtaccactc
     3481 ataggacgtc acgaattaat tatatcagtt tctttggcgt aaggtacgag taaaaatctg
     3541 ccaatttgca ttttgcttta ttgagtcggt aaagttttgt atactacagg tgcgcgtata
     3601 gttttctttc tgcagcggga agaccccgtg cttcagtcgg agttcaaggc gaaattatga
     3661 atctaaaata tcctgttgat cacacaaaat acattatttt tcaagaattt ggcaatgaca
     3721 atacaaataa ccaaacaaga aaagaattct acaccgtttt tgataataaa catcctggcg
     3781 tggatttctt aacacctgta ggtgtgacag tgcgggcttc atttgaaggt attgttgtaa
     3841 gaaaagaatt tcataaggga atggggaatg ttattggaat aagaaatggc aatattgtgg
     3901 ctctgtatgc tcatttaaag gatttttcag ttgaacttgg agatattgtt aaacaaaaac
     3961 aagatgttgg gcattcagga gaaaccggat cggcttgtat acttccccat ttacactttg
     4021 agctaagaga tatatcaaaa aattccttga aggagatggt ttttaagccg gaatttcttt
     4081 cggaagtctc gttatatagt gagcactttt attatactgt taacaataca aatacccaaa
     4141 aaaccttagc caagctgtca gtgctttact ttgggactac gaagtactgg atgaatattg
     4201 ctcagtttaa taagctaagt cagtctcaag acgaagacct tcagaatggt aaaaagcttc
     4261 taatacctaa ctactgaaat ttttgtgaat cttatttgta ttttcatagg cctttgtttt
     4321 taatgccgga agacttgcta agacgcgttt ttatttcaag ctaaatgttt aaactaacat
     4381 tcttgtgaat aatcaaaaca tagttttgca catcgagata gtacctcgga tgttgcacgt
     4441 ttgtcgtttt gatcatctga atagaaacaa aatataaaag gattttttga acgaattaaa
     4501 ccaatttcgt gcaccgcttg ctccaaagac ccgtttttat aagaaatatc tatattgtta
     4561 ggtaaataaa gaggtattct agtcctatca agctgttctt gcataatctt taaaatttgc
     4621 tcagagccga ataactcatt tttatacatc ttttctaata aaatccccat gtcgttgggg
     4681 gtggtcaagt ttgggccttt tcctgctttt atcatcatct tgtgcctaac cgttgtattt
     4741 tttaatccaa cattttttat gaactcttct acattttcag gatctactat atccagaatc
     4801 tggttagttg ccgtgttgtc tgaaacaata agcattaaag tgagcaagtc tttgtacgtg
     4861 agtaatgctc cgttgtcaat aagttttaat atgccggaac cttttacata attgctctcc
     4921 tcaatagaaa ttctatctgt aagtttaagc tcgttatttt ttgtgaatgc caaaaaagca
     4981 agtgcgatag gaacttttat agtacttgct gcccagaatt gatcgttttc attgtgttta
     5041 tactcttgtt cagagtttag gaattttaca tataggccaa cctgtccacg tagtttcgag
     5101 gtttcttttg aaagggattc tttgagatct aaaaatagca tatattgatt atatacattt
     5161 ttagtacgga ggaaaactta aaagattaca gttcgtatct gcatgagatt atatttcagt
     5221 gcaatttttt attgaaggcg tatcggggtt tattgattta taatttcgtt acttaaaatt
     5281 acctgcgaaa gctagcatat gtgtttaacc aacttttggg ctttgcaaca taaagaaatc
     5341 acagaaaatc gtcttattaa gatggtggtt ggtttaaatt ttacgttcgg aaaatttctt
     5401 ggtaatagtc agcaactttt ctgtagtttt tcatataacc ctcgctgggc ctcgtagtaa
     5461 atctacagct tttgaaactc cagaatttga attatcgcca ctcaaggcta cgcttaaatc
     5521 atggagcgca agatagtaaa ttaacgaggc cctagcgtac gggtcattta cattattaat
     5581 atagtctacg cctggctcca ggcacattac agatgcgcgt gcctctatac gagttagttc
     5641 cctttcttca gtggaaagcg cggcgagccc aggactgact atgaggctgc taacttggtg
     5701 taatgtatca aaaaaacgcg tttcagaatt ttttatatgt tcctttagat ctttctcaag
     5761 caggagagtt cttataattc cgtaccccaa aaccatttgc caggccgatg taggaaaaga
     5821 accttcgttt tgggctgcct ctagcccaag ttcgatgaat attttaaatg tctctgatcc
     5881 cgtaacagct tcatatagtc ggcttcctaa agcttctctc ataagtcttc tgagatcttc
     5941 aggtaaagtt ttctcttccc ctttagctac aaattcgggg ggttttttgt cggctaacc
//
LOCUS       DAOSWN010000039         5944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_8828_length_5944_cov_5.165563, whole genome shotgun sequence.
ACCESSION   DAOSWN010000039 DAOSWN010000000
VERSION     DAOSWN010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5944
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8828_length_5944_cov_5.165563"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            323..940
                     /locus_tag="PLF29_02465"
     CDS             323..940
                     /locus_tag="PLF29_02465"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HXK52823.1"
                     /translation="MISLLFSEPLVFALLFGVLVFSLTVHEYMHAAVADRLGDSTARL
                     LGRVTLNPSAHLDPIGTLLLLVAGFGWGKPVPFNPINFKNPKRDIAIVAVAGPLSNFA
                     MALVFGLILQLLPVALPQLILSVFTLLVYYNVALGVFNLLPINPLDGFKVVNGFLSHE
                     MSIQWLQLAPYGIYILLFGFFTNTIEKLISPIISAITGFILRAPF"
     gene            1263..2054
                     /locus_tag="PLF29_02470"
     CDS             1263..2054
                     /locus_tag="PLF29_02470"
                     /inference="COORDINATES: protein motif:HMM:NF014881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HXK52824.1"
                     /translation="MELFLAKDYTPKLDSLIKVRVGTGNWGRLAIIKIGSNQASAKYV
                     SLKTAYCDSMGIQVKVYEFDETPPKEEYLKIVNDPKNSSVIVQLPLPASCPNSYLDFI
                     APSKDVDFLSDLRREIFFAGDHSVLPPVVRAFELFLKQISPKKMTSAVVGEGYLVGKP
                     IRSYLQNQGFEVTAFNETQDLSNYLFVQDVVVLATGSARFFSGKNFADEVNVVDFGTS
                     RAPDGSLAGDLNLNSDIRHLGLVAPSPGSMGPLVIRYLVMNHLKM"
     gene            2135..3265
                     /locus_tag="PLF29_02475"
     CDS             2135..3265
                     /locus_tag="PLF29_02475"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HXK52825.1"
                     /translation="MSLNPYSLYCSVYRNIARPLLFLTPSEKIHERALETGEKISNNK
                     KAAGFIRNLCNYDNSALEQEILGVKYRNPVGLAAGFDYDGNLASFMSSLGFGFNTVGT
                     VTALPYEGNTGPRLGRLVKSKSLLVNKGFKSEGAEKIFSRLAKKDLSNITLGISIGSS
                     NLPEIDTTEKAINDYSSTFNMFNDVEYVDYFELNVSCPNTKIKEPFTNCPNLSALLSS
                     LSTLKIKVPMYLKLPNEISAADLFPLIDLALGKGVSGVILSNLVKDRSNPMLDEKEIA
                     KVSSLKGNFSGKPAAVNANNLIGEVYKRYGKDLVIVGCGGVFNAKDAYEKIKLGASLV
                     QLVTGLVYEGPTVAANINMGLVELLRKDGYENISEAIGVGNA"
     gene            3387..3845
                     /locus_tag="PLF29_02480"
     CDS             3387..3845
                     /locus_tag="PLF29_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52826.1"
                     /translation="MLVSVISIFLIIAGLVVGLGAVTVIDLHGFCARKSSYWTVATIA
                     THKITKPLIWLGILLLSLGLTFFYSSSIFLPRVAKMQLFLIAALVLNGVYLSFVISPA
                     LLIKEKQGKASELLSSSMQSRIAASMLVSFFGWWALVLSIAYIFSRLWQN"
     gene            3833..4519
                     /locus_tag="PLF29_02485"
     CDS             3833..4519
                     /locus_tag="PLF29_02485"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HXK52827.1"
                     /translation="MAKLIGSKIKHVVLDADGTLFKTENPYLYVAQALNVYEDVLVLI
                     NKYLSKDISYEELIEKETSLFSRTYKKMTGRHPHTGDLEKYLPVPKLFDDVKETIEEL
                     HRRQVHVYVISSGFYYLVEDLVALGIKLRDIHANKFLYNTHGEFEMIKIDVSGEKIDI
                     MKSIFGNSLGDVAYVGDNAFDEKLCEFVLLSEGTVFFKTTGENLFKMKHSFANKNFHK
                     TKTLSDILSF"
     gene            complement(4684..5064)
                     /locus_tag="PLF29_02490"
     CDS             complement(4684..5064)
                     /locus_tag="PLF29_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52828.1"
                     /translation="MKLINKILIANVVIITILLTGAIYTYYQPDTAQNKEKLQNLAEV
                     ENTKTTIDNNLTKTLDTGQKPETLSNLETCIITINSQKYDVTNFKDLHSGGDIFECNT
                     DMTAVFNSQHGRGILRQMEKFKIN"
     gene            complement(5061..>5944)
                     /locus_tag="PLF29_02495"
     CDS             complement(5061..>5944)
                     /locus_tag="PLF29_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXK52829.1"
                     /translation="WKISHQFMGVVLVFVGLHIGTITSDVSRNEPLKIWMLSVVAVGI
                     SAYMYKLIIYKNFTSKYNYVVDEIIKKGSIFELILNPKNENRPMIFEAGQYVFMRTAD
                     FKIGPEEHPFSISSPTNSKKIRLAIKILGDYTEQLSKIQKGAKLYLYGPYGDFSSKIN
                     TTPRIIAIAGGIGITPFLSILGSNMTDPATKMHLFYCTNNETDAVYKNELIATTKETS
                     NIEIIFWYSSIKGKIQAAKTLAPEMGVFMQDARKDVRNTLVMLCGPTEMMASLAKQFI
                     ELGVPRKNIVFEDFALK"
BASE COUNT         1650 a         1052 c         1222 g         2020 t
ORIGIN      
        1 aaatcttatt ggattcatat ttcttaggca gttacacgct cttttcgcaa gcagtaattt
       61 attgcactat tttaacattt ccacgccaaa aaaaaaggac tgatttttta actactacag
      121 ccggtcatag ccaatgctca caaatgcttg ttgccacgtg caaagcaaag gcacaaaagt
      181 agcttaaagt attttgggtt tgcgcatggc acgcggttgc cgatctcatt ctaagactag
      241 ctaagtcagg cctgtgtctt atttaggtta actttcattc tcgggtagcg atgtatatta
      301 aatctgttaa aatagtattt atatgattag tttattattt tcagaaccac ttgtgtttgc
      361 gctattattc ggagttcttg ttttctctct cacagtgcat gagtatatgc atgccgcagt
      421 tgcggacagg ttaggggatt cgaccgccag acttcttggt agagttaccc ttaatccctc
      481 tgcccatctt gacccgattg gaactctgtt gcttttggtt gctggtttcg gttggggaaa
      541 gcctgtacct tttaacccca ttaatttcaa aaacccgaaa cgtgatatag ccattgttgc
      601 agttgcgggt ccgttatcaa attttgcaat ggccttagtc tttggactta ttttgcagct
      661 gcttcctgtg gccctgccac aattaattct aagcgttttt acattgcttg tttactataa
      721 tgtagcgctt ggagttttta accttttacc tattaatcct ttagacggat tcaaagttgt
      781 aaacggtttt ttatctcacg agatgtctat tcagtggctt cagcttgcgc cttatggtat
      841 ttatatcttg ttgtttgggt tctttactaa cacaatagaa aagctaattt caccaataat
      901 ttccgctatc acagggttta ttttgcgtgc tcctttttag gttgttttaa agcatttttt
      961 tgtatcttga caaccgtttt ccgagtagta aaattataac taatgagtaa tctcattcac
     1021 ctgacgtgaa cgcttacgct gaagaagtaa gttttctcag caagtatctc ttctgggatt
     1081 cgagataact ggcataataa ccagttttag aaacccacct taagaaatcg agatgcaact
     1141 ttcagcgccc tgtcacgttg ggtacgaaat tattagacta gaagccaaaa aaatccgcga
     1201 ttgcggattt tttttgcgcc ggtcttgctt ttgatctccc atagttataa cttagatacc
     1261 aaatggaatt atttttagca aaagattata cgcctaaatt ggacagcctt ataaaggtta
     1321 gggtcgggac gggtaattgg ggtcgcttag cgattataaa aataggctct aatcaagcat
     1381 cggcaaagta tgtaagttta aagaccgcat attgcgattc tatgggcatt caggtaaaag
     1441 tttatgagtt cgacgagacg cccccaaaag aagaatattt gaaaatagtg aacgatccta
     1501 aaaattcgag cgtgattgtc cagctgcctc ttcctgcaag ctgcccaaac tcatatctag
     1561 attttattgc cccctccaag gatgtggatt ttctttctga tcttaggaga gagatctttt
     1621 tcgccgggga tcactctgtg ctgccccctg tagttagggc ttttgagtta tttctaaagc
     1681 aaatttcacc aaaaaaaatg acctccgcag tagtagggga gggctatctt gtaggtaagc
     1741 ccataagaag ctatttacaa aatcaaggtt ttgaagtcac tgctttcaat gagacgcaag
     1801 acctctctaa ctatctattt gtacaggatg tggttgtttt ggccacaggt tccgcaaggt
     1861 ttttttctgg caaaaacttt gctgatgagg ttaacgttgt tgattttggt acttccagag
     1921 cgcctgacgg gtcattggcg ggggatttga acctgaattc ggatattagg catttgggcc
     1981 ttgtggcacc ttctcctgga agcatggggc ctcttgttat taggtatctt gttatgaacc
     2041 atttaaaaat gtaggcctgt catagctagg cttactggtt tcttgctttt ttgccccttt
     2101 gcatatcgcc cctgttagtc gtaaaattaa aacaatgagt ttaaacccct acagtctata
     2161 ttgttctgtc taccgtaata ttgccaggcc gttgcttttt ttgactccgt cggaaaaaat
     2221 acacgaacgc gctttggaaa ccggtgagaa aataagcaac aataaaaagg ctgcaggctt
     2281 tatacgcaat ttgtgcaatt atgataactc agcactagag caagaaattt taggcgtaaa
     2341 ataccgaaat cctgttggat tagcggccgg atttgattat gatggaaatt tggcgagttt
     2401 tatgtcttct ttagggtttg gctttaatac tgttggaaca gtaaccgcct taccctatga
     2461 aggtaataca ggcccaagat tgggacggtt ggtaaagtca aagtcgcttt tagttaacaa
     2521 aggattcaag tctgaaggtg cggaaaaaat attttccaga ctagctaaaa aagatctcag
     2581 caatattact ttaggtataa gtataggtag ctctaatttg cctgaaatag atacaaccga
     2641 aaaagccatt aatgattata gcagcacctt caatatgttt aacgacgttg aatacgttga
     2701 ttatttcgag ctaaatgtta gttgtccaaa cacaaaaatt aaagaacctt ttactaattg
     2761 tcctaacctg tcagccttgt taagctccct aagcacttta aagataaaag tgcctatgta
     2821 tctgaaattg cctaatgaaa tttccgcagc ggacctattt ccgctaatag atttggcctt
     2881 gggaaaaggt gtatcaggag ttatattgtc aaacttagtt aaagataggt ctaatccgat
     2941 gctcgatgaa aaagaaatcg caaaagtctc ttctttaaaa ggtaacttta gcggtaagcc
     3001 cgctgcagta aatgcgaaca atttaatagg cgaggtttat aaaagatatg gcaaggatct
     3061 tgtgatagta ggttgtgggg gtgtttttaa tgccaaagat gcttatgaaa agataaaact
     3121 tggtgcaagc ttggttcagt tggtgacggg gcttgtttat gaagggccta cagttgcagc
     3181 aaatataaat atggggctgg ttgagttgtt aagaaaagac ggatacgaaa acatttctga
     3241 agctataggt gtaggtaatg cataaaagca ttgagttaga acataaattt gtctatgcag
     3301 tttcggatat ggcaggcgaa gtatacttta agctttagtt ttgctattgc tgtatagtta
     3361 gttatagaaa gggagggcac gtgcagatgt tagtttcggt tattagcatc tttttaataa
     3421 tagccggttt agttgtagga ttaggtgcag ttacagttat agatttgcac ggtttctgtg
     3481 cgaggaaatc ttcttattgg actgtggcaa ctattgctac gcataaaatc acaaagccgt
     3541 taatctggct aggcatttta ttactttcct tgggattaac ttttttttat tcgtcttcaa
     3601 tttttttgcc ccgggttgca aaaatgcaac tttttcttat agcggcgctt gtacttaacg
     3661 gagtgtattt aagttttgta attagccctg ccttgctcat taaagaaaag cagggcaaag
     3721 cttcagaact tctttcttcg tcgatgcaga gcaggatagc tgccagcatg ctagtgtctt
     3781 tttttggttg gtgggcgcta gttttatcta tagcgtatat ttttagcaga ctatggcaaa
     3841 actaataggt tctaaaatca aacatgttgt tctagatgcc gatggcacgt tgtttaaaac
     3901 tgaaaaccct tacttatatg tagcccaggc cttgaacgta tacgaagatg tcttggtttt
     3961 aataaataaa tatctcagta aagatatctc ttatgaagaa ctgatagaaa aagagacgtc
     4021 tttattttct cgtacttata aaaaaatgac cggccgccat ccccatacag gtgacttgga
     4081 gaagtacctt cctgttccca agctgtttga cgacgttaaa gagaccatag aagagcttca
     4141 tcggaggcaa gttcatgttt atgttattag ttctgggttc tattatttag tagaggattt
     4201 ggttgcgttg ggtataaaac taagagatat tcatgcaaat aaatttttgt ataacacgca
     4261 cggggagttt gaaatgataa aaattgatgt ttccggagag aaaattgata tcatgaaaag
     4321 tatatttggc aattctctgg gtgatgttgc ttatgtcggt gataatgctt ttgatgaaaa
     4381 gctttgcgag tttgtgctat tatcggaagg cactgttttt tttaaaacaa ccggtgaaaa
     4441 tttatttaaa atgaagcact cttttgcaaa taaaaatttt cataaaacta agactttgtc
     4501 agatatctta tctttttgat ttatttataa tttcgttact caaacctact tacgcgagct
     4561 agtagatata gtttacccaa catcttgagc tctgcaaggc aaagaaatta cagtaaaaca
     4621 cctgcttcag ctggtggtgg ttaatatctg cactgatccg aaaatatatt gaagtgtaaa
     4681 tttttagtta attttgaatt tttccatttg tcttagtatt cctctgccgt gctgactatt
     4741 aaaaacggct gtcatatctg tattacattc gaatatgtct cctccactgt gtaagtcttt
     4801 aaagtttgtc acgtcgtatt tttgtgaatt tatggttatt atgcaggttt ccagatttga
     4861 gagggtttct ggtttttggc cggtatcaag agttttggta aggttgttgt caatagtagt
     4921 ttttgtattt tctacttcgg ctaagttttg taatttttct ttattttgcg ctgtgtccgg
     4981 ttgatagtaa gtatatatgg ccccggtgag taaaattgta ataattacta cattagctat
     5041 taaaatctta tttatgagtt tcattttaat gcaaaatcct caaaaactat gttttttctt
     5101 ggaactccta attctataaa ctgtttggct agcgatgcca tcatttctgt tggcccacag
     5161 agcatcacca gcgtattcct tacgtccttg cgggcatctt gcatgaaaac tcccatttcc
     5221 ggtgctagag tttttgcggc ttgaatttta cctttaatgc tagaatacca aaaaataatt
     5281 tcaatattgc tcgtttcttt cgttgtcgct ataagttcgt ttttatatac agcatcggtc
     5341 tcgttattgg tgcagtaaaa caaatgcatt ttggtagcgg gatctgtcat attagatccc
     5401 agtatgctta aaaagggcgt tatgcctatt ccaccggcta tagctattat ccgtggggtt
     5461 gtatttattt tggagctaaa atccccgtag ggaccataca ggtacagttt tgcgcctttt
     5521 tgtattttgc tgagctgttc ggtatagtcg cctaaaatct taattgccag tctaattttc
     5581 tttgagtttg ttggagacga gatagagaag gggtgctcct caggtccgat tttaaaatca
     5641 gctgttctca tgaatacgta ttgccctgcc tcaaaaatca taggtcgatt ctcgttttta
     5701 ggatttagta taagctcaaa aattgaacct tttttaataa tttcatcgac tacatagttg
     5761 tacttcgagg tgaagttctt gtaaattatc agtttataca tataggcact tatgcctact
     5821 gctacaacgc tcagcatcca aattttaaga ggctcgtttc gtgaaacatc gcttgttatt
     5881 gttccaatgt gtaggcctac aaaaacgagc accaccccca taaattgatg gcttattttc
     5941 caca
//
LOCUS       DAOSWN010000040         5777 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_9217_length_5777_cov_5.123733, whole genome shotgun sequence.
ACCESSION   DAOSWN010000040 DAOSWN010000000
VERSION     DAOSWN010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5777
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9217_length_5777_cov_5.123733"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..143
                     /locus_tag="PLF29_02500"
     CDS             <1..143
                     /locus_tag="PLF29_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HXK52830.1"
                     /translation="LYFFKYGCPPHGGIGFGPTRMLMKIFDISNVRDVTYLYRGVKRL
                     TP"
     gene            173..1408
                     /locus_tag="PLF29_02505"
     CDS             173..1408
                     /locus_tag="PLF29_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52831.1"
                     /translation="MGNYKIVALANFFLGLLFVALSIIQSMIVSPRITQVTLEAGLNT
                     SSYFDYFYVLAGILFCFGIANLVFGFNLFIKKSENPNLLKSSKRGLNLLILSVLVLAV
                     LVPVSAVSLTSPFYSTANTSGNSKSGKINNPSQDKNIFFGSGEQKITDTSRPRSAAFM
                     REGDIYFLDIENSGDPVKISKTPKVSSPKLSPDGKYVYYFEIDHSTGGMPVYQIFVSD
                     VLGEQEKTFSMLANQSGSKPLWSEDSTMLGVLLYDMAGELPQLQKANIYDTKTQELIY
                     LGYATLPPGIQDEYAIAGSCSQVGQKYIDFCKKYTAYVKVPRVGDFLDQTLYRVSKYT
                     EEGYKLSRATRIGDNKIVLEYYTGDPKNPEAFWGEGGGSYVVGYDAGVTETYTLLVDE
                     GSGQVLYRLPQASEVDFLF"
     gene            complement(1440..1742)
                     /locus_tag="PLF29_02510"
     CDS             complement(1440..1742)
                     /locus_tag="PLF29_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52832.1"
                     /translation="MPPKTDPLGEVFVTIGKGSLGFMGFRDLTEQSTHLKNIKLWLYL
                     NEKYPANDSLKPFYLFTYDSHKVNNLLKDEWLYDVYYVNYPLNIHISPRVYLYKVE"
     gene            complement(1855..2496)
                     /locus_tag="PLF29_02515"
     CDS             complement(1855..2496)
                     /locus_tag="PLF29_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52833.1"
                     /translation="MRDDFAAFAKNNANVVALVEDWELKDFKNQEEALEQKGERGLIM
                     YWAKNLNVKVIPTDFDHNTCKHIKVLATEFQYEDIVLFIFLYRVKSQTLDKWIEKENL
                     EEYLVEKVNSNLECASLPKTTFDELQKLHIAKFDQTIQERSHVKAWEDLSVKKPDNGV
                     NTIFKAFHTLRDRNFLYQIVANYEQGKNVYVIYGCDHAQFHRPILDKLFNQTQ"
     gene            complement(2710..3231)
                     /locus_tag="PLF29_02520"
     CDS             complement(2710..3231)
                     /locus_tag="PLF29_02520"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HXK52834.1"
                     /translation="MIESTTYYNGHPVKLVWHPLNNLEGMAPIVQVYGLCFTQDGQVL
                     IIRNPLKAGTFTPWYLPGGTTEKGEKPEETLNREIREEADIEITNLKLLGAQEVFYPN
                     NPNKEKGDHYYQVRYFALISKTNPQTIDPHDGGIVELKYIDPKDFTKYVEWGEIGQEL
                     INLATKEFKKLVL"
     gene            3492..4022
                     /locus_tag="PLF29_02525"
     CDS             3492..4022
                     /locus_tag="PLF29_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52835.1"
                     /translation="MKWIKNEHKQETQNQNQILSNFEKAVEIAQSKKSRLVDMYMDNL
                     IDKDEFSVKKAEVESEEASARKELKILQASTSNWMDLAINTFNFAKTAKYSFEHGSAE
                     DKTAILRALGSNFTIMNGKVLLDLQKTFLQFKINSELVNAELKSLEPDVFVAVTNNNK
                     VFEGWFFKWSGRSDKS"
     gene            complement(4137..4772)
                     /locus_tag="PLF29_02530"
     CDS             complement(4137..4772)
                     /locus_tag="PLF29_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HXK52836.1"
                     /translation="MDKVQKFLSKLSTTNTPENLFNPFNEVCEIHDLPNAPQIRLENV
                     KTLLNKHLELGTKTVWLFEAPSYLGARRSGAPFVNEGMFEEVQSILGIEKSFQKATKT
                     ESATAMTSKMAWKVANEFGLKPLIWESLMFHPHKKGEPMTNRKPSKQEMMEYKHFLTD
                     LLDMIKPERMIAVGRVAEQGLALMDIKAEYVRHPAQGGYREFRKKMRELYI"
     gene            5048..5458
                     /locus_tag="PLF29_02535"
     CDS             5048..5458
                     /locus_tag="PLF29_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52837.1"
                     /translation="MNKLITKDKYEIVPGRPSIVTNAVVKKLEHALRRGASITEACSY
                     AEISRQVFYYHMHVDEDFFDTMCIARDSLKLNALENIYYAIQEGDLRASMWYLSKWVP
                     FDISQREAEIKDYRKTEEFAHNLYKTLEIEMLAS"
     gene            complement(5575..>5777)
                     /locus_tag="PLF29_02540"
     CDS             complement(5575..>5777)
                     /locus_tag="PLF29_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52838.1"
                     /translation="SPAQNSKKGVALKAVGTKLTGTWWEDRYNISNTARVLTRAILLY
                     FINNTDKWNEDFLFHLDLGIKS"
BASE COUNT         1745 a          991 c         1097 g         1944 t
ORIGIN      
        1 atctatactt ttttaagtac ggatgccctc ctcatggagg tataggattt ggtcctaccc
       61 gaatgcttat gaaaatattt gatatctcaa acgttaggga tgttacgtat ttgtataggg
      121 gtgtaaaaag attgacaccc tgaccacgag tgagggagaa ttaagtgaag atatgggtaa
      181 ttataaaatt gttgctctcg caaatttttt cttggggtta ctatttgtag ctttgtctat
      241 aatacaaagt atgattgttt ccccaagaat tacccaagtt actttggaag cgggcttgaa
      301 tacttcttcg tattttgact acttctacgt cttagcaggt atcctttttt gttttggcat
      361 tgccaatctt gtcttcgggt ttaatttatt tataaaaaaa tctgaaaacc caaacctgtt
      421 aaaaagttct aaaagaggcc taaatttgct tatattgtca gtgcttgtgt tggcagtgct
      481 agtgcccgta tcggcagttt ctctcacgtc acctttttat agtacagcca atacttccgg
      541 aaattcgaag tcgggaaaaa ttaacaaccc ctctcaagac aaaaacattt ttttcggcag
      601 tggggagcaa aagattacag atacttctag gcccaggtcc gcagctttta tgcgggaagg
      661 tgacatatac tttttagaca tagaaaatag cggtgatccg gtgaaaattt ctaagacgcc
      721 taaggttagt tcgccaaaac tatctcctga cggaaagtat gtttattatt ttgaaataga
      781 ccactcaacg ggaggtatgc cagtatatca aatttttgtt tccgatgttt taggagagca
      841 ggaaaaaacc ttttcgatgt tagcaaatca gtccggaagc aagcctttgt ggtcagaaga
      901 ctcaactatg ctgggagtac ttctttatga tatggcaggc gaactacccc agcttcaaaa
      961 agcaaacatt tacgacacca agactcagga gttaatttat ttaggctatg caactttgcc
     1021 tccgggtatt caggatgagt atgcgatagc aggttcttgt agccaagtgg gacagaaata
     1081 tattgacttc tgcaagaagt acacagctta tgtaaaggta cctagggtag gagatttttt
     1141 agatcagact ttgtaccgag tttctaaata taccgaagaa ggttacaaat taagcagagc
     1201 cacaagaata ggtgacaata aaattgtttt ggaatattac actggagacc ctaaaaaccc
     1261 cgaagctttc tggggagagg gcggggggtc ttatgttgtt ggatacgacg cgggtgtaac
     1321 agaaacttac acgttgttgg ttgatgaggg tagtggacaa gttttatata gactgcccca
     1381 agctagcgaa gtagactttt tgttttagat tttcctgcat tgcaataatg attttcgtat
     1441 tactctacct tgtatagata aactctaggt gagatatgta tatttaaggg atagttaacg
     1501 tagtacacat catatagcca ctcatctttt aagaggttat taactttgtg gctatcgtag
     1561 gtaaaaagat aaaaaggttt caaagaatca ttcgcaggat atttttcatt caggtaaagc
     1621 catagcttaa tattttttag atgcgtagat tgctcagtca gatcacgaaa acccataaag
     1681 ccaagggacc ctttaccaat agttacaaat acttctccga gaggatcggt tttaggaggc
     1741 acgtttatct ccagtacagt atcagcacga tgatactggc aagtttatta aagccattgc
     1801 agggcttaga ttggctgtat tatagcctat tttcacgcga atgacgacca taggtcactg
     1861 ggtttgatta aacaatttat ctaaaatagg tctgtgaaat tgagcatggt cacaaccgta
     1921 gataacgtaa acgtttttac cttgttcata gttagcaact atttggtaca ggaagtttct
     1981 atctcttaac gtgtggaaag ccttaaagat agtgttaact ccattgtctg gtttttttac
     2041 gcttaagtct tcccatgcct ttacgtgcga tctttcttgt atagtttgat caaattttgc
     2101 tatgtgaagt ttctgtaact catcaaaagt tgttttaggc aaacttgcac attccaaatt
     2161 cgagtttact ttctcaacta gatactcctc taaattttct ttttctatcc acttatctag
     2221 agtttgagat tttacccggt ataaaaaaat aaataagaca atatcttcgt actggaattc
     2281 tgtggctaac acttttatat gtttgcaagt attatgatca aaatcagtag ggattacctt
     2341 aacatttagg ttttttgccc aatacattat taagccgcgc tcaccttttt gttctaatgc
     2401 ttcttcttgg tttttaaagt cttttaattc ccaatcttct accaaagcca ccacgttagc
     2461 attattcttt gcaaaagcag cgaaatcatc cctcaacttt gtaataagtc tttgtaattc
     2521 atcgctcgat aaatgaactg tgtgagctgt tccaaaataa tgaattttat ttggtgtccc
     2581 aagttcataa atataaggag tatcaactcg aacaatatcc cagttttttt aagtcggaga
     2641 tataggcatc tatttgtgaa agctcgtcct ttaaatcttc tctatagtca attggaaaga
     2701 ggtgattttt cataagacta gctttttaaa ttctttcgta gcaaggttta ttaattcttg
     2761 gccgatttct ccccattcta cgtatttagt aaagtctttt ggatcaatat attttaattc
     2821 cactattccg ccgtcatgtg gatcaattgt ttgtggattc gttttactaa ttaaggcaaa
     2881 gtatcttact tgatagtagt gatctccttt ttctttgttt ggattatttg ggtaaaaaac
     2941 ttcttgagcg cctaagagct ttagattagt tatttctata tccgcttcct ccctaatctc
     3001 tctatttaga gtttcttccg gcttttcacc tttttcagtt gtgcctcctg gtaaatacca
     3061 gggagtaaat gttccggctt ttaaaggatt ccttattatt aaaacctgtc catcttgggt
     3121 aaaacacagt ccataaactt gaactatagg agccatacct tctagattgt ttaatggatg
     3181 ccaaacaagt tttacaggat gtccattgta gtaggttgtg ctttctatca tagagtcatt
     3241 ataagggtta ttatttttct ttgcataagt aatttaacgt aggagaatat cccttgcccc
     3301 ttatatgtct ataagcaatc ttgatgttac tttatcgttt taaaatctct gttgatatag
     3361 aacttttcaa tatatggtag tagtaactta agttgtgtaa tcagaagcaa actagtctta
     3421 atagtattga aaagcaattg ctagatattt tagacacagt gataatctca caaagttttt
     3481 gtgattgggc tgtgaaatgg attaaaaatg agcataaaca ggaaacacaa aatcagaacc
     3541 aaatactatc taactttgaa aaagctgtag aaatcgctca gtctaagaaa agccgcttgg
     3601 ttgatatgta catggataac ttgattgata aggatgaatt ctctgtaaag aaagcggaag
     3661 ttgaatctga agaagctagt gccagaaaag agctaaagat attacaagct agtacctcta
     3721 attggatgga tttagcgatt aataccttta attttgctaa aactgctaaa tatagctttg
     3781 agcatggctc tgcagaggat aaaacagcta ttctaagagc tttaggctcg aactttacaa
     3841 taatgaacgg aaaagtctta ttagacctgc aaaaaacgtt cttgcagttc aaaattaata
     3901 gcgaattggt aaatgcggaa ttgaaatccc tcgaacctga cgtttttgtt gctgttacaa
     3961 acaataataa agtctttgag ggctggtttt ttaaatggtc gggaaggagc gataaatctt
     4021 gaacaacttt taccgcttgc tgagtctatt tttgagaatt gggacattga aaaatttgat
     4081 gagggtctgc taactcctgt aggtgctggt gtggaacacg agtcaatcta caagtactaa
     4141 atataaagtt ctctcatttt tttcctaaac tctctgtaac caccttgtgc tggatgtcta
     4201 acatactctg cctttatatc cataagtgct agaccttgtt cagctactct tcctactgca
     4261 atcattcttt cgggtttaat catatctagc aaatcagtaa ggaaatgttt gtattccatc
     4321 atttcctgtt ttgagggttt tctgttagtc ataggttctc cctttttgtg tgggtgaaac
     4381 attaagcttt cccatatcaa aggtttcaag ccaaactcat tagctacttt ccatgccatt
     4441 ttagatgtca tagctgtagc tgattcggtc ttggttgctt tttggaagct tttttcaatg
     4501 ccaagtattg attgcacttc ctcaaacata ccttcattta caaatggagc acctgatcgt
     4561 ctcgcaccta gatagcttgg tgcctcaaat aaccacactg ttttagtccc caattctagg
     4621 tgtttattca acagagtttt aacattctcc agtcttattt gaggggcatt aggaaggtcg
     4681 tgtatttcac atacctcatt gaaaggatta aatagatttt ctggtgtgtt agttgttgaa
     4741 agttttgata aaaacttctg taccttatcc attacctaag tatatcaagc tcattaacga
     4801 aggataaagg ctataagatg actggtagga agtgagctaa ataatgccaa cactatgtct
     4861 ataagttatt gtggcgtaga aaattttatc gaaaggacgg ttttgcgtcg ctaattatag
     4921 cttatacgac atctatggaa gtgtatcacc ttttaaaaaa ttagtaattt agacaacaga
     4981 agttttaatg agtattcttc aagtagtaat ttagcctaca acaaggctac gtataggaaa
     5041 ccaaaatatg aataaactaa taacaaaaga taaatatgaa atagtaccag gcagacctag
     5101 catagttact aatgctgttg taaaaaaact agagcatgca ttacgtaggg gtgcatcaat
     5161 cacagaagct tgtagctacg cagaaatatc aagacaggtg ttctactatc acatgcatgt
     5221 agacgaagac ttttttgaca caatgtgtat tgctagagac agtcttaaat tgaacgcatt
     5281 ggaaaatatc tactatgcaa tacaagaagg tgatctaaga gcatctatgt ggtatttaag
     5341 taaatgggtg ccttttgata tttctcaaag agaggcagaa attaaggatt acagaaaaac
     5401 cgaagaattt gctcacaatc tatataaaac actagaaata gagatgcttg cttcgtaatt
     5461 gttaaatata attaacattt agttgttcac ttggctactg aggtcctatt aattgattct
     5521 tctagaacat gtaatgatgt aatagtagct atagatgata tgtattttaa ctcattagct
     5581 tttaatacca aggtctaggt gaaacaagaa atcctcgttc catttgtccg tgttgtttat
     5641 aaagtaaaga agaatagccc ttgtaagtac ccttgcagtg tttgaaatat tatatctgtc
     5701 ttcccaccat gttcctgtta atttggttcc cactgctttt agagccactc cttttttgga
     5761 gttttgtgcg gggcttt
//
LOCUS       DAOSWN010000041         5673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_9451_length_5673_cov_5.418120, whole genome shotgun sequence.
ACCESSION   DAOSWN010000041 DAOSWN010000000
VERSION     DAOSWN010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9451_length_5673_cov_5.418120"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PLF29_02545"
     CDS             complement(<1..448)
                     /locus_tag="PLF29_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52839.1"
                     /translation="MKEFENIKKYIEFINSLDLTFDKNPEEYGYKNPVILLMDATLSI
                     NRQYNKFVLPRIEYFKQSFPNINTLEKLNNLIKETGINDFENVWNYNHPKRVEILQNL
                     AKFFLEYKTENSIKDDLESMKHWAKSVDINSGKILPIDGMGFATSQY"
     gene            complement(569..1240)
                     /locus_tag="PLF29_02550"
     CDS             complement(569..1240)
                     /locus_tag="PLF29_02550"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HXK52840.1"
                     /translation="MKLILGGGGDENTSIQSHKRFFDLLKNSRKVLYIPIAILSDKYT
                     PEGCLSWFSGAFKNMGDLDIEMVTRFEDIDSDLGKYAGIYIGGGNTYKLLHEMKKSGI
                     DERLDTYKGVVYGGSAGAIIFGKDISTAEHADINNVGLKDISGLNYLKGYNLWCHYKE
                     EDIPLIDRLVEKGQKIISIEEDSAIYINNTHIIQSIGNNVRIHIQGKDTFKLLPKEQF
                     ELLPR"
     gene            1636..2103
                     /locus_tag="PLF29_02555"
     CDS             1636..2103
                     /locus_tag="PLF29_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52841.1"
                     /translation="MFKKYLSTLLALSLFINILFAYYFYSIKKINKQIEYSVVNDSFT
                     KDVEKDQDRVIEFTGEDNPIFLQGTLKKENIPDELELGDYWYWIYFEEPYLLMNNASG
                     LPLYIDKIQVLRPNSKDFYDLEKYIDKKVEIHGYQTWGYSESSVFQAIAIREL"
     gene            complement(2162..2235)
                     /locus_tag="PLF29_02560"
     tRNA            complement(2162..2235)
                     /locus_tag="PLF29_02560"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2201..2203),aa:Gln,seq:ctg)
     gene            3087..3677
                     /locus_tag="PLF29_02565"
     CDS             3087..3677
                     /locus_tag="PLF29_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HXK52842.1"
                     /translation="MNADTTKHPEQRVAIFADVQNMYYSAKNLYSGKVDFGRILTESV
                     GNRRLVRAFAYVIKADVGLEKEFFDALTQRGYEVKAKELQIFIDGSKKGDWDVGLCMD
                     VVRMLPKIDVMVLISGDGDYIHLLQYAKSQGVRTEVVAFSKTGSTKLFDEADDVIDMC
                     ADPKKYIIRKPRIPFIPKGVEPKPKNNAGAAKNDKS"
     gene            3741..5639
                     /locus_tag="PLF29_02570"
     CDS             3741..5639
                     /locus_tag="PLF29_02570"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HXK52843.1"
                     /translation="MFSRLRLGLVAFYLLCALLAFFAAYHVFFGRKIIPGVYVGVVDV
                     GGLTYGDAEKKLADTISSLPSELSFVFEDNVYTTSLSDLDITYNPSQSVQRALQVGRT
                     GNLYIDTKDKIAGLIGKKLTVPASLTHDEKALNNFFAVIVGTLDVQAKDARFSLDEEG
                     RLSLVESSAGFKVDHQNLYDQSLWSLNNFDFTQKILLVEKVYPKVNTVALKLFEPKIK
                     ALLKNEITLVPQAVPASNNSEGETQEKIKVTKDDEKDPESKNGNSKLGSESVSNFPNF
                     EPIILSDLEKLSLVEVSKVDDSYSLVINKPQFEAYTSAIAQEVNTLPRGKITAISNDI
                     VTEFEFVEPGYELEKKSFERNLKDALLWETPVDDIGLEEKKPTSVVLTFNKIEGAADP
                     SKYGIYKLIGEGTSKYTGSAPSRIHNLLLAAKRTDGVLVPPGEIYSFNTSVGKISSST
                     GYATAYVISNGRTVLGDGGGVCQTSTTLFRAALNSGLPIVTRHPHAYRVGYYEIESPV
                     GVDASIYQPSLDFEFKNDTPNFILIEAVPNEVEYSLTFKLYGTPDGRKVEISEPVVSN
                     QSPPPPALYQDDPTLPKGVVKQVDFAAWGATASFNRIVYKDDKKIIEDTFVTRYQPWR
                     AVYLVGTK"
BASE COUNT         1656 a         1066 c         1085 g         1866 t
ORIGIN      
        1 tatattggct tgttgcaaat cccattccat ctattggtaa tatttttcca gaatttatat
       61 ctacactctt agcccaatgt ttcatacttt ctaaatcatc cttgatagaa ttttcagttt
      121 tatattcgag aaaaaactta gctaggtttt gtaatatttc caccctctta ggatgattat
      181 aattccacac gttttcgaag tcatttatac ccgtctcttt tatcaaatta ttaagtttct
      241 caagagtatt tatattagga aaactctgtt taaaatattc aattcttgga agaacaaatt
      301 tgttgtactg tctatttatt gaaagggttg cgtccatcaa gagtattact gggtttttgt
      361 acccatattc ttccggattt ttatcgaaag ttaaatctaa tgagttaata aattctatat
      421 atttttttat gttctcgaat tctttcacga tattataata tcactatatc ccattcacag
      481 tttctccagg atatttggct gagcatgcgg gtctagtact aaaatagggt tctatcctgt
      541 acgatgcgta tccaggccat ttggttgttc atcggggaag aagttcgaac tgttcttttg
      601 gtaaaagttt gaaagtatct tttccttgta tatgaattct tacattatta ccaatacttt
      661 gaattatatg tgtattattg atgtaaattg ccgaatcttc ctcaatagat ataattttct
      721 gccccttttc tactagccga tctattaggg gaatatcttc ttctttatag tgacaccaca
      781 agttataacc ttttaggtaa tttaacccag aaatatcttt taaaccaaca ttatttatat
      841 ccgcatgttc agcagtagat atatctttac caaatatgat agctccagca ctgcccccat
      901 agactactcc cttatatgta tctaatctct catcgatacc agattttttc atctcatgaa
      961 gtaacttata ggtatttcca ccaccaatat agataccagc atacttgcct aagtcactat
     1021 cgatatcctc aaatctagtg accatttcga tatctagatc tcccatattt ttaaaagctc
     1081 ctgaaaacca agaaaggcac ccttctggtg tatatttatc tgaaagaatt gctattggga
     1141 tgtataagac ttttctagaa tttttcagaa gatcaaaaaa gcgtttatgt gattgtatgg
     1201 aggtgttttc atccccgcct ccgcccaaaa ttagtttcat aagaacatta tatcagttcg
     1261 aattacctga tggcctatat ttgggattgt tgttataggg agaaaccctg gctgggggac
     1321 agggattcgg tcacgcataa aattctgctg aatttttgcc cgacctcggc tcggcaccgt
     1381 cgtgcctgcg ccattcgaat cctttcgctc tcaacaagta atttggcaac cagcccttca
     1441 ggctgattgc caaattcctg gctgagcata tgggtctagc atcaaaaagg tagttctatc
     1501 ttgtacgtag tttcttaagg ctatatggct ggccgtatgg tcacaaattg gtacttataa
     1561 aaatataaac ccatttttta agtcaattgc acatatatgc tatttcgtac tgccataaag
     1621 tagtaaaata tgtttatgtt taaaaaatat ttatctactt tattagcact ttcgttattt
     1681 ataaatattt tatttgctta ttatttttat tccatcaaaa agataaataa gcagattgaa
     1741 tactcggtag ttaatgactc ttttacaaag gacgttgaaa aagatcaaga tagagttata
     1801 gaatttactg gggaagataa tccaattttt ttacaaggca cgttaaaaaa agaaaatatt
     1861 cctgatgagc tggagttggg agattattgg tactggatat attttgagga gccctatcta
     1921 ttgatgaata atgcttccgg attgccatta tatattgata aaatacaagt gttacgacct
     1981 aatagcaagg acttttatga tttagaaaaa tacatagata aaaaagtaga aattcacggt
     2041 taccaaacat ggggatattc tgaaagttca gtttttcaag caatagcaat tagagaactt
     2101 taatgtctaa accgttaaag aaaaacatcc gcaaacctct caggcttagg gatattttcc
     2161 ttggctgggg gacagggatt cgaaccccaa ttcctgcatc cagagtgcag tgtcctacca
     2221 ttagacgatc ccccaatgtg cccgacatta tatcgcgggc gagctagttt gttaacttct
     2281 tcgtaccttc tctttccgta tatccggaga tactacggct atcaactttc caaaaagcgc
     2341 ccttttcggc gtgctttttc ttttgttttt gggcagttat tctattgtat ggcatttaca
     2401 ttcgcaagtt tcgtcctctt tgcattcgca gcagcattcg tctttagagc aattgcactg
     2461 tccgttaccg ttgcaacagc agcctttctc tgtgtcctcg ggcgagcaat gtccgccgca
     2521 acaagaatcc tcgtggtttt ggcaacaatc gtcttttgcc cctccgcaac aagatttttt
     2581 atctcctatt ttaagtagat cctttatttt atttagcatt ttttcgcctc ccttctattt
     2641 atatatggtt ttttgtacct tttttagtta ctacttttaa cactgaccgg cccgtagttg
     2701 ggaagttgca agctgtcttt atgtaggcag atcgtcgttt gtttcaaaca gtagcagttg
     2761 tattgtaata cgtatccttg catatggcta tcaactacca ccagcttacg caggtggctt
     2821 actgtaattt ctttgccttg cagagctcaa catgttgggt aaattacatc tactagctcg
     2881 ggtaagtagg tttaagtaac gaaattatca actatcacca gcttacgcag gtggtttgct
     2941 gtaatttctt tgccttgcag agctcaacat gttgggtaaa ctacatctac tagctcgggt
     3001 aagtaggttt aagtaacgaa attataaatc tcagttgctt taagtagttt ttcgcatctt
     3061 tagagtaatt ctgttaaaat gaccgtatga atgctgacac aacaaaacat ccggaacaga
     3121 gagttgcgat ctttgctgac gtacaaaata tgtattactc cgctaaaaac ctatattcgg
     3181 gaaaggtaga ctttggccga attttaactg agtctgtggg aaaccgccgt ttagttaggg
     3241 cttttgctta tgttatcaaa gcagatgttg gtttagaaaa ggagtttttt gatgctctta
     3301 cccagcgcgg gtatgaggtt aaggcaaagg agctccaaat ttttatagat ggttcaaaaa
     3361 aaggtgactg ggacgttggt ctatgcatgg atgttgttag aatgcttcca aaaatagatg
     3421 ttatggtttt gatatcaggc gatggtgact acattcactt gttgcagtat gcaaaaagtc
     3481 aaggcgttcg aacagaggtt gtagcgttta gcaaaaccgg ttcaaccaag ttgtttgacg
     3541 aggctgacga tgtcattgat atgtgtgcgg atccaaagaa atacataatt agaaagcctc
     3601 gtattccttt tattccaaaa ggggttgagc ctaagcccaa gaataatgca ggggctgcaa
     3661 agaatgataa gtcctagtta tccaataagg tttttggagt tcggatgcta tttcgcaaat
     3721 ttttattact aagttcaaaa atgtttagtc ggcttagact tggcttggta gctttttatt
     3781 tactttgcgc tttgcttgca ttttttgcag cctaccatgt tttttttggt cgcaaaatta
     3841 ttcctggagt atatgttggt gttgttgatg tagggggttt aacttatggt gatgccgaga
     3901 aaaaactcgc ggatacaata agttctttgc cttctgagct atcttttgtt tttgaagata
     3961 atgtttacac aacctctttg tctgatttag atattactta caatccgtca caatctgtcc
     4021 aacgagcttt gcaagttggg cgcaccggaa atctttacat agacaccaaa gacaaaattg
     4081 ccggcctaat aggtaaaaag ttaactgtac ctgcctcttt aactcatgat gaaaaggctt
     4141 taaacaactt tttcgcagta atagttggaa ctcttgatgt gcaggcaaaa gacgcccgtt
     4201 ttagccttga tgaagaaggc cgtttgtctc ttgtagaatc ttccgctggc tttaaggtag
     4261 accaccaaaa tttatatgat cagtctttgt ggtcactaaa taattttgat tttactcaaa
     4321 aaattttatt agtagaaaaa gtgtatccta aggttaacac cgtcgcactt aaattgtttg
     4381 agccaaaaat aaaagccctt ttaaaaaatg aaataacttt agttcctcaa gcagtacctg
     4441 caagcaacaa tagcgagggc gaaacgcaag aaaaaataaa agtcaccaaa gatgacgaga
     4501 aggatcccga gtcgaaaaac ggtaatagca agttagggag tgaaagtgtt tcaaattttc
     4561 caaattttga gcctattatt ttatcggatt tagaaaagct tagtttggta gaagtttcca
     4621 aagttgacga ttcgtacagc ctggtcataa ataaacctca gttcgaagcc tacacctcgg
     4681 ctattgctca ggaggtaaat acattaccgc gagggaaaat aaccgctatc tcaaacgata
     4741 tcgtaacaga gtttgagttc gttgagcccg gctacgaact tgaaaaaaaa tcattcgaga
     4801 gaaacttaaa agacgcgttg ttatgggaaa ctcctgttga tgatattggt ctagaagaga
     4861 aaaaacctac ttctgttgtt ttgaccttta ataaaataga aggtgcggca gatccctcaa
     4921 agtacggcat ctacaaactt attggcgagg gaacttccaa atataccgga tcagcccctt
     4981 cccgaattca taatttgctt ttggcggcca agagaaccga cggagttctt gtaccgcccg
     5041 gagagattta ttcatttaat acttcggttg ggaaaataag tagttctacc ggatatgcta
     5101 cggcctatgt tatatctaac ggtcgtactg ttttgggaga cggtggggga gtgtgccaaa
     5161 catccacaac tttgtttcgc gcagcgctaa attcgggctt acccattgtt actaggcacc
     5221 cccatgccta tcgtgtcggt tactacgaaa ttgaatcgcc cgttggagtt gacgcctcaa
     5281 tatatcagcc atcgcttgat tttgaattta aaaatgatac gccaaacttt attttaatag
     5341 aagcagttcc caacgaagtc gaatattcct taacttttaa attatatgga actcccgacg
     5401 gtcggaaagt tgaaatttcg gaaccagttg tttccaatca atctcctcct ccccctgcgt
     5461 tatatcaaga cgacccaact ttgccgaagg gtgtagttaa acaggtagat tttgctgctt
     5521 ggggagcaac ggccagtttt aacagaatag tatataaaga tgataaaaaa ataattgaag
     5581 acacttttgt aaccaggtac cagccgtgga gggctgttta tttggttggt acaaaataaa
     5641 aacctactct ctagataaag ttacactaag tat
//
LOCUS       DAOSWN010000042         5660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_9475_length_5660_cov_4.523996, whole genome shotgun sequence.
ACCESSION   DAOSWN010000042 DAOSWN010000000
VERSION     DAOSWN010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9475_length_5660_cov_4.523996"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            531..740
                     /locus_tag="PLF29_02575"
     CDS             531..740
                     /locus_tag="PLF29_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52844.1"
                     /translation="MSTKLQKKTFQIRLDIGWWKILSNLRTNSGQSFKTLVEYALSET
                     YNGYETESELKKIFGKKKPKKYKFY"
     gene            complement(1306..1962)
                     /locus_tag="PLF29_02580"
     CDS             complement(1306..1962)
                     /locus_tag="PLF29_02580"
                     /inference="COORDINATES: protein motif:HMM:NF018053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1045 domain-containing protein"
                     /protein_id="HXK52845.1"
                     /translation="MTTTPVTNSINTVFSANSDIENETLIDAKRFMNEHYGNKYSHIP
                     PHISYTIMPLPEYNFERAKDDLIAYVIKQKPYLLKLSDLKFDNRDNLFYIEVSGKDMQ
                     RLHEDITILLNKHREGFIREKDLERISKGYFNDNELEYLRQYGYSRVFECFRPHISIG
                     NFTVENVDVPELTKILKEKLTGVLNKSLVLNNIRALFHTDAGSQQEAEVFWESNYLLS
                     "
     gene            complement(2015..3094)
                     /locus_tag="PLF29_02585"
     CDS             complement(2015..3094)
                     /locus_tag="PLF29_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine monophosphate reductase"
                     /protein_id="HXK52846.1"
                     /translation="MPKTKLLEQNMSMALSFDDVLLVPQRSKVLSRSDVDLSTKITQE
                     LELKIPLISTNMDNITGVAMAIKMHELGGIGVLPRFESVESQVQKVKKVASVGAKVIA
                     SVGIKEGELERAEMLVLSGVSGLDVDVAHGHMSKAIDFTAMLKNKFGNKVAIIGGIAS
                     TGECAEDFYKAGADVVSVGIGGGSICSTRIQTGCGVPTLASLLDIAPIAKKYKKTFFP
                     LAGIKNSGDIVKSLAAGSCAIRGGNIFSGTDEAPGDIIEIDGRYYKRYNGSTSEEEKI
                     KQRNLGSNGKNKHYTIHIEGYEALVPYKGPVEDVVQRLLAGVRSGFSYCGASNIDELQ
                     RNATFIKITNAGISENGAHDVRVVK"
     gene            3230..3958
                     /locus_tag="PLF29_02590"
     CDS             3230..3958
                     /locus_tag="PLF29_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HXK52847.1"
                     /translation="MALQKYKKKFNYSYTFGAFPTIELLNKYPNQVLSVLLSSQGRMA
                     EGVSAVLQLCEKHNIRVDVGDKAISKVALKDNTYVVGVFKKYLTSLDVSSNHVVLDRP
                     SNTGNVGTVIRTMLGFGYKNIVLIKPCIDFFDPMVIRSSMGASFGVSYAYYSSVDDYM
                     SEYGSHTLYPFTLRAKNSLSTVSLTRPYSLLFGNEGSGLDSKFDSYPTSIRIDHSSLI
                     DSLNLSVSVALALYEFSKPLGDRQ"
     gene            complement(3984..5012)
                     /locus_tag="PLF29_02595"
     CDS             complement(3984..5012)
                     /locus_tag="PLF29_02595"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HXK52848.1"
                     /translation="MEIINKLQRGSFTAINSCLRVKQEDRVLIVTDLETKEVGESLYS
                     EAKKVCKNVKIVVIEDYTNRPAKSFPDKLKHEIDEFKPNISIYAAQGYEGELGAFRRP
                     LIDYFKQKYNCKHAHMISITKQLMEDGMNQDLEKVYDLTNKLLRIVEQAERIHVSDPH
                     GTNITFYFDSKLKWIPDDGRIEPGHMQNLPCGEVYTSPKTGNGRFVAWVLGDNFASQG
                     VLKSPLTVEIENGIIASTSCDDTELLENFNNYVNEHEFGNRVGEFAIGTLLGLTGFVG
                     NLLQDEKFPGVHIAFGYPYPHETGADWTCPSHLDIIAKDTTVTLWLKSGEEEKTIMVD
                     GKFIPELL"
     gene            5123..5614
                     /locus_tag="PLF29_02600"
     CDS             5123..5614
                     /locus_tag="PLF29_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HXK52849.1"
                     /translation="MRNKKLFALSLLAGLAFIGLIALFAKSNEQRFSTSPAKNSFIED
                     DVERSAQDAISEGLKDGQFSYLDYSPEILDRLNNKKVLLFFFAPWCPTCRAAEKEIME
                     KGAFELPKDLVVLKVDYDSSIALRKKYGVTVQHTFVQISGIGGEENSWIGGGVSAINQ
                     NLE"
BASE COUNT         1599 a         1104 c         1114 g         1843 t
ORIGIN      
        1 acaacaacat ttacctgttg acacaaatcg acttttatac gaaaatgaaa ttgtaaggct
       61 tgggtaaaac acccaaaagc tgagtccgtg actccaaata cagcctttac tgagttagca
      121 aatagaaatt gaaaacatca gtaaatgttt gtgtgaggag tttttttaat gggcatcatg
      181 ctattttctc cattatctaa actttcactc acaaataaaa acgcgcgcca cagaattgat
      241 gatttctgag tgatgtttct cacactcaaa agtcattaag acttacagta ggggctgcgc
      301 agtctaaagc ccgtgcaccg ataagggcct ggtcgaaaga agtatcacta actgcgtccc
      361 atacggaaac ctctgacggt gataaacaga tgatgaagag aaaaagttat gtaattacgt
      421 agaacacaca ggacccagac cttttctggg tgaggacaca aaagcacagg caagaacgtg
      481 aatcaccaga aaggaaacgg ctgggggaac taccccgtga ggggttatat atgtcgacaa
      541 agttacaaaa aaagacattt caaatacgtt tagatatcgg gtggtggaaa atactatcta
      601 atctaagaac taactcagga cagtcgttta agacgcttgt agagtatgct ttgagcgaga
      661 cctacaacgg atatgaaaca gagtctgagc ttaagaaaat atttggcaag aaaaaaccaa
      721 aaaagtataa attttattaa gattctaata tcttaaagcc aggcatctag agattagcaa
      781 tagccgatat ctatgacact agtgtacaca gtactgtagg tactactggg tacagtactg
      841 cgcggaattt tttatactac tatgcatact actatcgata ctagtatata tactactata
      901 catagtagta tcggattaac gttcaatcat acaaacagct acatcagcca aaacattaag
      961 actctctata taagacagct cagtggtaga gcagagggct tttacactga atcgttctga
     1021 ttcagtgaac gccttatgag cctgcgaatt aggcgttaac cagggtgtcg tgggttcgat
     1081 ccccaccgcc ctcaccatcg aaactctgta agctatggcg agcctcaagt agactataca
     1141 ttatatagcc ctggttacgg cctggagtac tatcgaggta aggctattgg acacgtcaga
     1201 cattaagccc taacacctac aatgctataa tccgaatcaa cataaagaag aaagggtgac
     1261 atatcaattt cgtagtcggg aaatttgttt gaccaactcc ctacactaag acagcagata
     1321 gttgctttcc caaaaaacct ccgcttcttg ttgactgccc gcgtcagtat gaaaaagtgc
     1381 tcgaatgtta ttaagtacta agcttttgtt taacacgccg gttaattttt ctttaagtat
     1441 ttttgtaagt tccggcacat ctacgttttc cactgtaaag ttgcctattg aaatgtgcgg
     1501 gcgaaaacat tcaaataccc tcgaataacc atactgtctt aggtattcca gctcattatc
     1561 gttaaaatat cctttagata ttctttctaa atctttttct ctaataaaac cttccctgtg
     1621 tttgttgagc aagatggtaa tatcttcgtg cagcctttgc atatctttac cagatacttc
     1681 tatataaaat aaattatccc tattgtcgaa tttaagatcc gagagcttta gcaagtaagg
     1741 tttttgtttt atgacataag ctattaaatc atctttggct ctttcaaagt tgtactcggg
     1801 aagaggcatt attgtgtatg agatgtgggg cggtatgtga gaatatttat tgccgtagtg
     1861 ctcattcatg aacctttttg catctataag tgtctcattc tctatatctg agtttgcgga
     1921 aaacacagtg tttatactgt ttgttacagg tgttgtagtc atggtacaaa ttatagcact
     1981 atagttatct ttctgtgttt tatattaatg gtttttattt aactactctg acgtcgtgcg
     2041 cgccgttttc tgaaattccg gcgttcgtta tcttaatgaa cgtcgcgttg cgttgaagct
     2101 cgtctatgtt agaagcccca caatagctaa atccagacct tacgcctgcc agcaatcttt
     2161 gaaccacgtc ctcaaccgga cctttatagg gaaccagagc ctcataccct tctatgtgaa
     2221 tcgtgtagtg tttatttttg ccgtttgagc cgagattacg ctgtttgatt ttttcttcct
     2281 cagaagttga tccgttatat cttttgtagt atcttccgtc gatttcaatt atgtctccag
     2341 gagcttcatc tgttccggag aaaatgttgc ctcctcttat tgcgcaagat cccgcagcga
     2401 gagatttaac aatgtctccc gaattcttta ttcccgctaa aggaaaaaat gtctttttgt
     2461 attttttggc aataggtgca atgtccagga gcgatgctag tgttggaacc ccgcaccctg
     2521 tttgaattct ggttgagcat atggatcctc cgccaatgcc aacggacaca acatctgctc
     2581 cggctttgta gaaatcttcg gcacattctc ctgtggacgc gatgcccccg ataatggcta
     2641 ctttgttccc aaatttattt tttaacattg ctgtaaaatc tatagcttta gacatgtggc
     2701 cgtgcgcaac atctacgtcc agccccgata ctccggatag aaccagcatc tcggccctct
     2761 caagctctcc ctctttaatt cccacagatg caatgacttt tgccccgaca gaagctactt
     2821 tttttacttt ttgaacctgt gattctacgg actcgaatct tggaagtacg cctattccgc
     2881 caagttcgtg catttttata gccatggcta cgcctgttat attatccata tttgtggaaa
     2941 ttaaaggtat ttttagctct agttcttgtg tgatttttgt tgaaaggtct acatcggaac
     3001 gcgataatac tttagatctc tgcgggacaa gtaatacgtc gtcaaaagat aaggccattg
     3061 acatgttctg ttcaagcagt tttgttttgg gcactattaa atattagacg cttttgtttt
     3121 tatcgtcaaa ccaaattaga gtaaaccact tgcgtaagct ggcagtggtt gatacatgac
     3181 ttagaaatgc agataaacta gctttggttt ttgtgatatc ttaaactaca tggcattaca
     3241 aaaatacaaa aagaaattta attattctta cacttttggc gcttttccca caattgaact
     3301 gttaaataaa taccctaacc aagtgttgtc ggttttgcta agttctcagg gtcgcatggc
     3361 ggagggagtg tctgctgtgc tgcagctttg cgagaaacat aatattagag tagatgtagg
     3421 agacaaggca atttcaaaag tagcattaaa agataataca tatgtggttg gcgtttttaa
     3481 gaagtatctt acttctttgg atgtttcttc aaatcatgtt gtgcttgata ggccgagtaa
     3541 tacaggtaat gtcggaaccg ttatcagaac gatgcttggt tttggttata aaaatatagt
     3601 tcttattaaa ccctgtatag atttttttga tcccatggtt ataagatctt ctatgggggc
     3661 gtcttttggt gtaagttacg cttattacag ctccgtggat gattacatga gtgagtacgg
     3721 ctcacacaca ctatacccat ttaccctgag agctaagaat tcactaagta cagttagctt
     3781 gactaggccc tactcgttat tatttggaaa tgaaggctca ggtttggata gcaagttcga
     3841 ttcttacccc acgagcataa gaatagatca ctcttcgctt atagattctt taaatttgtc
     3901 tgtttcagtt gcacttgcgc tttatgaatt ttcgaagcct ctcggagatc gtcaataact
     3961 cttagatttt gctttgattt aattcacaat aactcaggta taaatttacc gtctaccatt
     4021 attgtctttt cttcttcacc gctttttaac caaagagtta cggttgtatc ttttgcaata
     4081 atatccaaat gagaagggca ggtccagtcg gcacctgttt catgcggata ggggtaacca
     4141 aaagcaatat gaaccccggg gaatttttca tcttggagca aattgcccac gaagccggta
     4201 agtcctagaa gtgtgccaat agcaaactct ccgactctgt ttccgaattc gtgctcgtta
     4261 acataattat taaagttttc cagcagctct gtatcgtcac aagaagttga agctattatc
     4321 ccgttttcaa tctctaccgt aaggggggat tttaaaacgc cctgtgatgc gaaattatct
     4381 ccgagtaccc aggcaacaaa ccgaccgttc cctgttttag gcgatgtata aacttctcca
     4441 caaggcagat tctgcatgtg tccgggttca atacgtccgt cgtccggtat ccactttaat
     4501 tttgaatcaa agtaaaatgt aatgtttgtt ccgtgaggat cagaaacatg aattctttcg
     4561 gcctgttcta cgattctcaa tagcttgttg gttagatcgt aaactttttc taagtcctgg
     4621 ttcataccat cttccatcag ctgcttggtt atagatatca tgtgcgcgtg tttgcaattg
     4681 tatttctgtt taaaataatc gatgagtggt ctgcgaaatg ctccgagttc cccctcatag
     4741 ccttgggccg cgtagattga aatatttggt ttgaactcgt ctatctcatg ctttaatttg
     4801 tcagggaaac ttttcgccgg cctgttggtg tagtcttcaa taacaactat ttttacgttt
     4861 ttgcaaactt tcttcgcctc agagtataaa gattctccga cctcttttgt ttctaagtcg
     4921 gtaactatca gaaccctatc ctcttgtttt actcgaagac aactgtttat tgcggtaaaa
     4981 gaacctcttt gtagtttatt gattatttcc atttctaaaa tatactacat ttttatatgt
     5041 tttgcattgt cgtttgatat tgcaatattt gtattctatt aagtgatttt taattgatat
     5101 taaaataacc tatactatac atatgcgtaa taaaaaactt tttgcattat ctctattagc
     5161 cggtctagct tttattggat tgattgcact ttttgcaaaa agcaacgagc agagattttc
     5221 tacaagcccc gccaaaaatt cttttattga ggacgatgta gaacgttcgg cgcaggatgc
     5281 tatttccgaa ggcttgaaag atggccagtt tagttatctg gattattctc cggaaatttt
     5341 agataggcta aataacaaaa aggttcttct tttctttttt gctccgtggt gtccaacttg
     5401 cagagctgcg gaaaaagaaa ttatggaaaa aggtgctttt gagttaccaa aagatctcgt
     5461 tgttttgaaa gtagattatg attcttctat tgctttgagg aaaaagtatg gagttactgt
     5521 tcagcacacg tttgttcaaa taagtggtat tgggggagag gagaacagtt ggataggtgg
     5581 tggcgtttct gcaatcaatc aaaacctgga ataaagtaag atttaatatg aaaaggtatt
     5641 ttattttggt gcttttgttg
//
LOCUS       DAOSWN010000043         5618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_9570_length_5618_cov_4.587992, whole genome shotgun sequence.
ACCESSION   DAOSWN010000043 DAOSWN010000000
VERSION     DAOSWN010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9570_length_5618_cov_4.587992"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1640)
                     /locus_tag="PLF29_02605"
     CDS             complement(<1..1640)
                     /locus_tag="PLF29_02605"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HXK52850.1"
                     /translation="MKKKFKDLTTSPDFPDLEKQKLELWKSLKAVESLKELRKDSVEK
                     VYYDGPITANGMPHYGHAITWTMKDVIPRYWSMKNYLVSRNIGWDCQGIPVEFEIEKE
                     MGFEHKDDIEEFGVAKFNEMCRQSVLKHRDAMFAYESRLGRWFDESDMYYTMDASYIE
                     SMWWALKELYNKGFLYQGYKVVAYSTRAGTSLSTHEVQDGGYKELEDPSVTLKFKLID
                     EDAFVLAWTTTPWTLPSNLMLAVGKKVVYAKVQYEGETYYVAEARLEDVFKNNSYKVL
                     EKLSSSDLEGREYEPLFDYYLSKRAEGCFRIVIADHASDEDGTGIVHLAPYGAEDFDV
                     FMALGIQLFDYLDESARFTDEIPELKGKFYKDANPIILESLEKKGLLFDSSTILHRMP
                     ICWRTGTPLIYKPIRSWYLATTKLKERMLEENQKVNWKPEHTKNGASKIWLENVRDWA
                     LSRSRYWGTPLPVWINDKTGDIHVIGSFQELEELSGVKLEDPHKPYVDEVTWDDKSSG
                     GTFRRVPDVVDVWFDSGAVPFAKLHYPFENQDRFKETFP"
     gene            complement(1954..2601)
                     /locus_tag="PLF29_02610"
     CDS             complement(1954..2601)
                     /locus_tag="PLF29_02610"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HXK52851.1"
                     /translation="MKIYTKKGDSGKSSIINGGILSKKEYVFEVLGTMDELNAALALA
                     VVDNKSKISRTILGLQEDLFSLGAVIASYESSGDGDVSESKKRAGTALAKSVKWTRTS
                     SLGPDVRAMVLKSERLIDELSSDLPELKNFILPGGSIQAKNLHISRAVCRRLERSFVS
                     FVSQKDFVIDDVSRDMLIYFNRLSDLLFVLARYANHVQQIEDTIWLPKKFNEKVK"
     gene            complement(2721..5171)
                     /locus_tag="PLF29_02615"
     CDS             complement(2721..5171)
                     /locus_tag="PLF29_02615"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HXK52852.1"
                     /translation="MKVNTTFIKDYGLQKKEDIELSPNSQNILERRYLRKNPDGTYAE
                     NIQQLFERVASTVAKPDAPYRDVQKSTVEYYNLLATKRFFPNSPTFTGAGTPLGQLAA
                     CFVLPVSDDLGIGEDSIFSTLRTAALIQQSGGGNGFSFSKLRPKGSIVKSSNGKASGP
                     VGFMSVYDGAFGEVAQGGARRGANMGVLKVDHPDIREFIMCKAQEGKMANFNISVAIT
                     DRFMEAVRNDTAYELIDPNTKEVTETPMAREIFDLIVKHAHKNGEPGVLFIDEANRTN
                     PVPHLYDLEATNPCGEQWLGPYENCCLGSLNLEAHLNEDGTLNWELLEESVELATRFL
                     DNVVSENTYVPSIPQLREAALNARRIGLGFMGLADVMYAMGVRYGSLEGQELAAQITE
                     FIRYHSMKISIALAKERGPFLKIKGSIYDPENLTWQPPKPLTPYSKDFGRPALDWAYV
                     VEGIRTHGIRNAAQTTVAPTGTISTVAGIEGYGCEPVFALAYLRNVYQAAGDSEKLTL
                     TYVSPMFEKALDMLDLSPETRQNIISSVANTGSCTDIEDLPREIRDVFVVSSDITAEE
                     HIMTQAAIQAFIDNSISKTCNFPEGATLEDVSKAYMLGWEMKCKGLTVYVTGSRTEVV
                     LETKAVKDSKEAGSYTGPEEVKNVNPEPIMDRGYVLNGNTYKVQTPQGKAFITVNRDA
                     NGDPFEVFVGVGKAGSDVSALSEALGRSLSGWLRTSYNPLTTAKEIVSQLIGIGGARS
                     VGFGKSRVSSIPDAIAKVLADDLKIKVKANGSNIIEENSNNISVNAHLDMCPDCGNSS
                     LIREEGCLKCHDCGYSVC"
BASE COUNT         1629 a         1300 c          981 g         1708 t
ORIGIN      
        1 gggaaggtct ctttaaaacg atcttgattc tcaaatgggt agtgcagttt ggcaaaaggc
       61 acagctcctg aatcaaacca aacatcgaca acatcaggta ctcttctaaa agtgcctcca
      121 ctagacttat cgtcccaagt cacttcgtcc acataaggct tgtgaggatc ttcgagttta
      181 acaccggaaa gctcttctaa ctcttggaat gagccaataa catgtatatc tcctgtcttg
      241 tcgttaatcc aaaccggcaa gggtgtaccc caatacctac ttctgctcag cgcccagtca
      301 cggacatttt ccaaccaaat cttcgaagca ccgttttttg tatgttcagg cttccagttc
      361 actttttggt tttcctcaag cattctttct tttagttttg tggttgctaa gtaccagctt
      421 cttattggct tataaatcaa aggggttccg gttctccagc atatcggcat tctgtgtaga
      481 atagttgagg aatcaaacag caggcctttt ttctctaaag attccagaat tatcggattt
      541 gcgtctttat aaaatttgcc tttaagctct gggatttcat cagtaaaccg cgcgctctcg
      601 tccaggtaat caaaaagttg tattcctaaa gccataaaaa catcaaaatc ctcagcgccg
      661 tagggggcaa gatgaacaat gcccgtgccg tcctcatcgc ttgcatgatc agcaataacg
      721 attctaaaac aaccctcagc tcttttactc aagtagtaat caaacaaagg ctcgtactca
      781 cggccttcaa gatcagaact tgaaagtttt tctaatactt tatatgaatt attcttaaaa
      841 acgtcttcta atctagcctc agcaacataa tatgtttcac cttcatactg cacttttgca
      901 taaacaacct ttttccctac cgccagcatt aaattagaag gcagggtcca cggggttgta
      961 gtccatgcca atacaaaagc gtcttcatct ataagtttga actttaatgt gactgaagga
     1021 tcttcaagct ctttgtagcc accatcttga acttcatgag ttgataagct cgtgccagca
     1081 cgtgttgaat aagcaacaac cttatatcct tgataaagaa agcctttgtt gtaaagttct
     1141 tttagagccc accacatgct ttcgatatag cttgcgtcca ttgtgtaata catgtcggat
     1201 tcatcaaacc aacgtcccaa acgactttca taagcaaaca tggcgtcacg gtgctttaga
     1261 acagattgtc tgcacatctc gttaaattta gcgacaccaa actcttcaat atcgtctttg
     1321 tgttcaaagc ccatctcttt ttctatttca aactcaaccg gaattccttg gcaatcccaa
     1381 ccaatatttc ttgaaactag gtaatttttc atagaccaat atcttggaat aacgtctttc
     1441 atggtccagg ttatagcatg accatagtga ggcatgccgt ttgcagttat aggcccgtca
     1501 taataaactt tttcgactga atcttttctc aactccttca aactctctac agcttttaga
     1561 gatttccata actctagctt ttgcttctca agatcgggaa aatcaggact tgtagtcaaa
     1621 tctttaaatt ttttcttcat aaaaaccccc taaaaaagaa aaacccttta ctaataaagg
     1681 gttaaagcga gccaaaccga aatacggccc gcgatctaaa taataattat tgtttttgaa
     1741 acttttgtat tcataaccta cacttgacta tacaaaaaat atttctttaa tgcaaataaa
     1801 catttttttc tctaggtaaa gcttgtgact ctaaagtgct agcggatacg caataattat
     1861 atttactgca gtaaggcagc caacctagtt cacgtatgaa aacttatgta cgtagaagta
     1921 aagaaaggtc ttctgctctg ggtccgcaac acttcatttt actttttcat taaacttttt
     1981 gggcaaccaa atagtatctt caatctgctg aacgtggttt gcataccttg ctaaaacaaa
     2041 caataaatca gacaatctat taaaataaat tagcatgtct ctggaaacat catctataac
     2101 aaagtctttc tgagaaacaa aagatacaaa actccgctca agtctcctgc acactgctct
     2161 ggaaatatgt aaatttttgg cttgtatact gccgccaggt aaaataaagt tttttaattc
     2221 cggtaaatct gaggacagct cgtcaatcaa acgttccgac ttcaaaacca tcgcgcgaac
     2281 atctggccct aatgaagagg ttcttgtcca tttgactgat ttggctaaag ctgtacctgc
     2341 tctttttttt gattctgaaa catcaccatc gcctgaagat tcataagaag ctatcacagc
     2401 accgaggcta aaaagatctt cctgcagtcc taggattgtt cttgatattt ttgatttatt
     2461 atcaacaaca gctaaagcca aagcagcatt aagctcgtcc atagtgccta atacttcaaa
     2521 aacatattct tttttcgaaa gaattccgcc gtttatgatt gaggatttgc cggagtcgcc
     2581 tttttttgtg taaattttca taatttattc tgctataaaa ataggggtgc tacaaggcct
     2641 atttattgaa ggaggcaggg agattttctc cctgcctcct tcttaataac aaacaatcaa
     2701 gcccggacat aagcgcctaa ttaacagaca ctatagccac aatcatgaca ctttaaacaa
     2761 ccctcttctc ttataagcga agagttgccg caatccggac acatatctaa gtgggcatta
     2821 acacttatat tatttgagtt ttcttcaatt atattcgaac cattcgcctt tacttttatc
     2881 tttaaatcgt ctgccaatac tttagctata gcatcaggaa tggaacttac cctgctctta
     2941 ccaaaaccta cagaacgcgc gccgccgatg cctattagtt gcgatactat ttcttttgct
     3001 gtcgtcaaag gattataact tgttctcaac cagcccgata aagaccttcc aagagcctca
     3061 gagagagccg atacgtcgct acccgcttta cctactccta caaaaacctc gaaaggatcg
     3121 ccgttcgcat ctctgtttac agtaataaaa gcctttccct ggggagtttg aactttgtaa
     3181 gtatttccgt tcaacacata ccctctatcc attatcggct cgggattaac gtttttaact
     3241 tcctcgggac cggtataaga gccggcctct ttagagtctt ttactgcctt agtttccaaa
     3301 actacttctg tcctacttcc ggtgacataa accgttaatc ctttacactt catctcccaa
     3361 cctagcatat aagctttaga gacatcttcc agggtcgcgc cctcagggaa attacatgtt
     3421 ttagatattg agttatctat gaaagcctgt attgcggcct gagtcattat atgctcttcg
     3481 gcagtaatat cagaggaaac aacaaaaaca tcacgaatct ctctaggaag atcttctatg
     3541 tcagtacagg aaccggtgtt tgcaactgaa gaaataatat tctgacgcgt ttccggagaa
     3601 agatctaaca tatcaagggc cttctcaaac attggactaa cataggtgag ggttagcttt
     3661 tcagaatctc ctgcagcctg ataaacgttt cttaaataag ccagagcaaa aacaggctca
     3721 cagccgtaac cctcaattcc tgcaacagtt gagattgtac cggttggggc aacagttgtt
     3781 tgagcagcat ttctaatacc gtgagtcctg ataccctcca ccacgtatgc ccaatccagc
     3841 gccggcctac caaaatcttt tgagtagggg gtcagcggtt tggggggctg ccatgttaga
     3901 ttttcagggt cgtaaatact acctttaatc tttaagaaag ggcctctttc ctttgccaac
     3961 gctattgaaa tcttcataga gtgatatcta ataaattcag ttatctgcgc tgccagctct
     4021 tgcccctcga gacttccgta ccttactccc attgcataca tcacgtccgc caaacccata
     4081 aaacctagcc caattctcct ggcatttaag gccgcctctc tgagctgagg aatagacggc
     4141 acataagtat tttcagatac tacgttatct aaaaatcttg ttgctaactc tacactttcc
     4201 tctaacaact cccaattaag cgtgccatct tcgttaagat gcgcctcaag gttgagcgac
     4261 cccaaacaac agttctcata aggtccaagc cactgctcgc cgcaaggatt tgttgcctcc
     4321 agatcataaa gatggggcac ggggttggtt ctatttgcct cgtcgataaa taacactccg
     4381 ggctcaccat ttttgtgtgc gtgtttaact attaaatcaa atatttcacg agccattggt
     4441 gtctcggtaa cctctttagt attaggatct attaattcgt aagctgtatc gtttctaacc
     4501 gcctccatga atctgtcggt aatagccacg gagatgttga aattcgccat tttaccttcc
     4561 tgggctttac acataataaa ttcccttata tccggatgat caacctttaa tacacccatg
     4621 ttagcacccc gacgagctcc tccctgagct acctcaccga acgcaccatc atacacggac
     4681 atgaacccaa ccgggccgct agccttgccg ttactagatt ttacaatact gccctttggt
     4741 ctaagctttg aaaatgaaaa gccgtttcca cctccagact gctgaattag tgccgcagtt
     4801 cttagcgtag aaaaaatgct atcttcacca atcccaagat cgtcagagac cggcaaaacg
     4861 aaacaagcgg ccaactggcc aagcggggtt ccggcaccgg taaaagtagg ggaatttgga
     4921 aagaaacgct ttgtcgccaa aaggttatag tattcaaccg tagatttctg tacatctcgg
     4981 taaggagcat cgggctttgc aactgtggaa gcaacgcgtt caaatagttg ctgtatattt
     5041 tcggcataag ttccgtcggg attttttctt aaataccttc tctccagtat attttgggag
     5101 tttgggctga gttctatgtc ttcctttttt tgcagaccgt aatctttaat aaaagttgta
     5161 ttaactttca atgaactgag tatactaggt ttaaaagcgt tagaggcaac attaatccac
     5221 ttcatattaa agaggatttc agaaaccggt aggcttaaac tgcctgaaag actacaggtt
     5281 tatggaatcc ggtcaggaat tttatacttt tttgtttcaa gtcttatctt ttcataatca
     5341 aaaaaacaat accactaatg ccgattaata cttgaactat tttgaactac tgtggaaatt
     5401 aaaaatcaat aaacaggtat tttttttaac cgatcctata ttgcaacatt ctaaatttta
     5461 acatttatga ttttctatta ctgctaaagg gtttgaaagc tcttaataac tctaagcaac
     5521 caccagctga cgcaggtgat ttgctgtaat ttctttgctc tgcaaagccc aacatattgg
     5581 gtaaactaca tctacaagcc tacacgagtc gttttaag
//
LOCUS       DAOSWN010000044         5532 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_9791_length_5532_cov_5.665875, whole genome shotgun sequence.
ACCESSION   DAOSWN010000044 DAOSWN010000000
VERSION     DAOSWN010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9791_length_5532_cov_5.665875"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="PLF29_02620"
     CDS             complement(<1..955)
                     /locus_tag="PLF29_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52853.1"
                     /translation="MKARVFALLVIYYQKIRNNTSSRLVQFVILNLVLTLYNVIAPIL
                     KLDQINERVPLFYYMPWGNLQLVPKNYIFLVPSINLVLIGTGVLLYFVAKKYQFQYLD
                     MLSIFTSSISNVLLTLSLYRSIYISSTPESISAGSAAIQFISPFFIALGLAYLLTPKF
                     ISVLTERGVVTNPLLHRHPGMLLAKPSARGGGFIFYLVFLAVSVVTVGFTKELTGIYL
                     VAGILATLGLLDDFQNTNAKSRLKFIENPVLRLALLYITSYLFILFGISIKYIGNPAG
                     GIFDFNSFSIVINNTPVFFLSVTITLLWVVWVLNLLSWSNGI"
     gene            complement(963..2513)
                     /locus_tag="PLF29_02625"
     CDS             complement(963..2513)
                     /locus_tag="PLF29_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52854.1"
                     /translation="MVNKFALKYDKQTQRFFEILPGTLTWAMLLSPFWLGLLAPNVFI
                     FYITFLTVLWVYLAGTSAYGVMLGYKKYQQELNVNWIEECANLNFETLPDHNTLPPSL
                     KDLKIFVLIPVVNEPRQVLSAMLEGLQAQTFPSEQTALIFALEEKFAEEIEKTIRDLL
                     KNDLSKFGEILFFRHPANIEGEVKGVAGPNRTWGAKHAVEVLKEKNENLRNYIFYTMD
                     ADHVLHEQFLARLAHLYLTSDKRDHKFYTTAVHLFNNNIWEVPTLPRIEANSITLGTL
                     SEWAQGKAHVKDTFANYATSLTTLIDADYWEVKIGIIDDAVFFWRAFFVRKGDFIGVP
                     HYIPFSADAVQEQNYLRTHKSMYKQLLRWGWGVVKVPMSLKGFLQRGDIPLSVKLVWT
                     KRHLETSVIYLTTVYLMTFGLTLLTLVNPRAAQASFSYTLPDVVSFLLTVALVFFVPL
                     TVFRSRIVKPIPKDWPLWRRLLVLFEGPMVVINLFTFSFLPFIEAQTRMMLGNKMKDL
                     YHTPKVRK"
     gene            complement(2518..3315)
                     /locus_tag="PLF29_02630"
     CDS             complement(2518..3315)
                     /locus_tag="PLF29_02630"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HXK52855.1"
                     /translation="MILIDTHAHFPKSKTRTKDILNRAAEQDVKYCIGIGTDLKENDS
                     IINHALMFEHLYAGIGIYPNSEMAKRPATLTEQLEKQIKRANSGSKKVVIAIGECGID
                     ITEWHNQRPLKEQLELFELQILLAQKLTLPVIIHNRNGDEHILKLLQKYNNQNLQGVV
                     HCFDSTLEFANKILELNFYISFTGFLTFNRKSYLADIVKEIPLNRILIETDSPYIVPK
                     GVKEERNEPKNVKMVAQRLADIKGLSVEQVAKETTNNASKLFEIAFN"
     gene            3341..4459
                     /gene="tsaD"
                     /locus_tag="PLF29_02635"
     CDS             3341..4459
                     /gene="tsaD"
                     /locus_tag="PLF29_02635"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007504449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HXK52856.1"
                     /translation="MIILGIETSCDETAAAVVVDGREELASSLATSEELHQSTGGVVP
                     EVAARKQLEFFVPVLEDVCIKAAPKLGLSNYTEVLNKVDAIAVTVGPGLIGSLLVGVN
                     GAKALAFAHNKPLVPVNHLVGHIYGNFLRPLQHSDSNSSDHIAPATYLDNFSNEENIF
                     PALVLIVSGGHTDLVLMKGHGSLEYLGGTVDDAAGEAFDKVARMLGLSSYLGGPLISK
                     AAAECRHNSLLGQLPRPKIADEDYDFSFSGLKTATKNLINSYSSKDSPLPLNEICCEF
                     EIAVVDVLIAKTKKAAQANNIKSILLGGGVSANKQLRTRLLGLGQELGVFVSVPPIRL
                     CTDNAIYIASAAYYNYRPVSADKLDLIKANPGLGIVDQ"
     gene            complement(4638..5465)
                     /locus_tag="PLF29_02640"
     CDS             complement(4638..5465)
                     /locus_tag="PLF29_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52857.1"
                     /translation="MAIRIFASTFNILFGTNEKVFESIKRLSEMGASNDLAFLAFQEV
                     RKNPKIKDIKEKLEASLEQKNLSYYMHHADTVNHLGLALASNIKYTTTHNLKLPQLNK
                     LPWRIQSYFLDQMPLCGAIINTYNIEGKVLGVVNMHLDIGGGFKHKLNQVRSVKNYLE
                     TLHCDYEIIMGDFNTIGTVKVLFPLIRWQSKKIQQLFGTDYKITNTEKTYTSDIRGVV
                     NPALPLKKLVEFTKKKGFAWEQKLDWIITKNLIIEKSTVRHDLIGSDHYPVCAVLKI"
BASE COUNT         1565 a         1058 c         1139 g         1770 t
ORIGIN      
        1 ctatgccgtt tgaccaagaa agtaaattta gcacccatac tacccacagc aatgtaatgg
       61 ttactgaaag aaagaatacc ggtgtgttgt ttatcactat gctgaagctg ttaaagtcaa
      121 aaatacctcc ggctggattc ccgatgtact taattgagat tccgaacagg ataaaaagat
      181 aagatgttat atatagtagg gctagcctta gtaccgggtt ttctataaat ttcagcctgc
      241 tctttgcgtt tgtgttttgg aaatcatcca gtaagcccag tgtggcaagt attccggcta
      301 caagatatat tcctgtaagt tcttttgtga aaccgactgt aactacagat actgccagaa
      361 agaccaaata aaaaataaat ccgccacctc tggcagaggg tttcgctaga agcatgcccg
      421 gatgcctgtg taacagtggg tttgtaacta ctcccctttc ggttagcaca cttataaatt
      481 ttggtgttag taggtatgca agtcctaggg caataaaaaa aggtgagata aattgtattg
      541 ctgcagagcc ggccgaaata gattccggag tagacgatat atagatacta cgataaagag
      601 aaagcgtgag aagaacatta gatattgaac ttgtaaagat actgagcatg tccaagtatt
      661 gaaactgata ttttttagca acaaaataaa gtaaaacccc tgttcctatt aacactaggt
      721 ttatgctagg tacgagaaaa atataatttt tcggaaccag ctgaaggttg ccccacggca
      781 tgtagtagaa aagaggcacc ctttcgttta tttggtcgag ctttagtatg ggcgcaatta
      841 cgttgtaaag agttaaaact aggtttaata ttacaaattg caccagccgg cttgaggtgt
      901 tatttcttat tttttggtaa taaatgacta aaagtgcaaa aacccttgct ttcatagtag
      961 tattattttc tcactttcgg ggtgtgatat aaatccttca ttttatttcc gagcatcatc
     1021 ctggtttgtg cttcaataaa aggtaaaaac gagaatgtaa ataagtttat gactaccatt
     1081 gggccttcga acagaaccaa caatcttctc cacagaggcc aatctttagg aataggtttt
     1141 actatgcggc tgcgaaagac cgtaaggggc acgaaaaata ctagggctac cgttagcaag
     1201 aagctcacta cgtcgggcag ggtgtagctg aagcttgctt gcgctgctct cggattgacc
     1261 aaagtaagca aagttaggcc aaaggtcatt aaataaactg tggttaaata aattacgctg
     1321 gtttccagat ggcgttttgt ccagaccaac tttacggaca aaggtatatc tcctctctga
     1381 agaaagcctt taagagacat aggaactttt actactcccc atccccacct taaaagctgt
     1441 ttgtacatgc ttttatgggt tcttaaatag ttttgttcct gtacggcatc ggccgagaaa
     1501 ggaatataat ggggaacacc aataaagtct ccttttctta caaaaaacgc acgccaaaaa
     1561 aatactgcat cgtctataat tcctattttt acttcccaat agtccgcgtc aattagtgta
     1621 gttaaagagg ttgcatagtt agcaaacgta tcttttacgt gggcttttcc ttgagcccat
     1681 tcagacaacg tccctaaggt aatagagttg gcctctattc taggcagggt aggaacttcc
     1741 cagatgttgt tgttgaatag atgaaccgct gttgtataaa atttgtgatc tcttttatcg
     1801 ctggtgaggt agagatgcgc caaacgagct aggaattgtt cgtgcaaaac atggtctgcg
     1861 tccatagtgt aaaaaatata attacgtaaa ttttcattct tttcctttaa aacctccaca
     1921 gcatgttttg caccccatgt tctatttggc cctgccacgc ccttaacctc accttctatg
     1981 tttgcggggt gtctaaaaaa aagaatttcg ccaaacttgg ataaatcatt cttaagcaaa
     2041 tctcttatag tcttttcaat ttcttctgcg aacttttctt ccagagcgaa tatcagagcc
     2101 gtctgttctg acggaaatgt ttgcgcctgc aggccctcca acatagctga tagtacttgt
     2161 cgaggctcgt ttacaacggg aataagaaca aatattttta gatctttaag tgacggaggc
     2221 aatgtattat gatcaggaag tgtttcaaaa ttaaggttag cgcattcttc tatccagttc
     2281 acattaagct cttgttggta ttttttatat cccaacatga cgccgtatgc agatgtacct
     2341 gctaaataca cccacagaac agtaagaaaa gttatgtaaa aaatgaagac attaggagcc
     2401 aagaggccca gccaaaaagg cgaaagcaac atagcccaag ttaaggttcc gggcaggatc
     2461 tcaaaaaacc tttgagtctg cttgtcgtac tttaatgcaa atttattaac catatcttta
     2521 attaaaagca atttcgaata atttagaggc gttgttggta gtctcctttg ctacctgctc
     2581 aacagaaagt ccttttatgt ctgctaagcg ttgcgcaacc attttaacat ttttgggctc
     2641 gttacgctct tctttaactc ctttgggcac aatataaggg ctatcggttt ctatgagaat
     2701 tctgttaagt ggtatttctt tgactatgtc agctagataa gactttcggt taaatgtcaa
     2761 aaagccggta aaggatatat aaaaatttag ttctaggatt ttgtttgcaa actcgagtgt
     2821 gctgtcaaaa cagtgtacta ctccttgcaa attctgattg ttgtattttt gaagcaattt
     2881 taatatgtgc tcgtctccgt tacgattgtg aataataacc ggtaaagtaa gtttttgcgc
     2941 caataaaatt tgaagctcaa aaagttctaa ctgctctttt agcggtcttt ggttgtgcca
     3001 ctctgtaata tctattccgc attctccgat tgcaataaca acctttttgc tgcctgagtt
     3061 tgcacgtttt atttgttttt ctagctgttc agttaatgtt gccggccttt tcgccatttc
     3121 actattaggg taaattccga ttccggcata aagatgttca aacataagag cgtgattgat
     3181 tatggagtcg ttttctttga gatctgtgcc tattccaatg cagtatttta cgtcctgttc
     3241 cgcagctctg ttaagaatat cctttgttcg tgtttttgat tttggaaaat gtgcgtgagt
     3301 atctataaga atcatactta agatattgta caatatgcat atgattattc taggtattga
     3361 gacttcgtgc gacgaaaccg ccgccgcagt tgtggttgat gggcgtgaag agctcgcaag
     3421 ctctttagct acctctgaag agttacacca aagcacgggg ggcgtggtgc cggaagttgc
     3481 ggctcgcaaa cagctggaat tttttgtacc agttttagag gatgtgtgca ttaaggcagc
     3541 gcctaagtta gggctgagta attacacaga agttttaaat aaggttgatg caattgcagt
     3601 cacggttggt ccgggactca tagggtcgtt gctggttggg gtaaatggtg caaaagcttt
     3661 ggcttttgca cacaacaaac ctcttgttcc cgtaaatcat ctggtaggcc acatctatgg
     3721 aaatttttta cgcccattac aacattccga ttcaaatagt tcagaccata ttgctcctgc
     3781 cacctacctc gataatttct ctaacgaaga aaatatcttt cccgctctgg ttttaatagt
     3841 aagcggcggt catacagatt tagtacttat gaaagggcac ggtagtttag aatatttagg
     3901 aggcaccgtt gacgacgcgg ccggagaggc ttttgataaa gtagctagaa tgttgggttt
     3961 aagcagttat cttggtgggc ctttgatttc caaggcagct gccgaatgtc ggcacaattc
     4021 tctgttgggg caactaccaa ggcccaaaat agcagatgaa gattatgatt tttctttttc
     4081 gggtttaaaa acagcaacca aaaatcttat taattcttat tcttcaaaag actcgcccct
     4141 gcctttaaat gagatttgct gtgaatttga gattgcagtt gtagatgttt tgattgccaa
     4201 gaccaaaaaa gccgcacaag ccaacaatat aaaaagtatt ttattgggtg gaggggtttc
     4261 ggcaaacaaa caactaagaa caaggctact tgggctgggc caggagcttg gggtttttgt
     4321 tagcgttccc cctatacgtc tatgtaccga taatgctatt tatattgcta gtgctgctta
     4381 ttataactat cgccctgtgt cggcagacaa acttgatctc ataaaggcaa atcccggatt
     4441 aggaattgta gatcaataag gttttatagt agatatttcc tttttcacct catatcaacc
     4501 accaccagct tacgaaggcg gtttgctgta attttttgcg ttgcaaagcc taacatgttg
     4561 ggtaaaatac atctactagc ttatgcagga attttaagta gcgaagttat aaaattagaa
     4621 tatgccattt cgttctatta tatttttaag acagcgcata ccggatagtg gtctgaacct
     4681 attaggtcat gccgcaccgt gcttttttct ataataagat ttttagtaat aatccagtcc
     4741 agtttttgct cccaagcgaa acctttcttt tttgtaaatt caacaagctt ctttagaggt
     4801 agcgccgggt tcacaactcc ccgaatatcg gaagtgtatg ttttttctgt gtttgttatt
     4861 ttgtagtcag tgccgaaaag ctgctggatt tttttacttt gccatcttat aagtggaaaa
     4921 agaactttta cagtcccgat agtgttaaag tctcccataa ttatttcgta atcgcagtgt
     4981 aaagtttcta aataattttt tactgatctt acctggttta gtttgtgttt gaagcctccg
     5041 ccgatgtcca agtgcatatt taccacacca agaactttcc cctcaatgtt gtaagtgtta
     5101 attatagccc cacatagagg catctgatcg aggaaatatg attgtattct ccacggcagt
     5161 ttgttgagtt gcggaagttt aagattgtga gtagttgtat actttatgtt agatgctaaa
     5221 gccaggccca gatgatttac tgtatcggca tggtgcatgt agtagctcag gtttttttgc
     5281 tctaggctcg cttcaagctt ttctttaata tcttttattt ttggattttt tcgtacttcc
     5341 tgaaacgcta agaacgctaa atcatttgaa gcccccatct ctgatagccg tttaatggat
     5401 tcaaaaactt tctcgttggt tccaaacagt atgttaaatg ttgaagcaaa tatgcgaatg
     5461 gccatgcgtt gtattataga cttttgcttt ggttttcgaa acccgcaaag ctagcgttcc
     5521 tactcctcgt ct
//
LOCUS       DAOSWN010000045         5213 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_10614_length_5213_cov_5.018418, whole genome shotgun sequence.
ACCESSION   DAOSWN010000045 DAOSWN010000000
VERSION     DAOSWN010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10614_length_5213_cov_5.018418"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="PLF29_02645"
     CDS             <1..1149
                     /locus_tag="PLF29_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52858.1"
                     /translation="SQCRGDIVGPYTGIMYAPEDPTHPKEISAATELAALTFRLPSQE
                     AYRTGSMTFHDIDPQNLLSDYDKERYAMNSGGSGVTTLQNVSSYVKVPNSTSYSGMEA
                     PELVDKGDDGTGTTPPPPGFDGKCNGSVFASTFLKNAPNLSPTTSDVPNVSPQVEGVY
                     AALEDVTGLACEIFAGLHYNETGGTNAPNRSIISGRVLGENEPDVGKTYPPCEEGDTT
                     FESCLPGLYESGVDAARIFIAHSGTSASNLDYYSSVRAFSRYTSPFYKNCGHKLTQYN
                     DPYVVYPGFAQYCSDPLPYEGYDNLPLNLIDSEHSNMGLMSCGFHPDFGNYVDICPIE
                     PEINYNNDPPTGPDGEDLIGPFDQWFRMGSATAAYRWYQAVTSSRAER"
     gene            1352..1678
                     /locus_tag="PLF29_02650"
     CDS             1352..1678
                     /locus_tag="PLF29_02650"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HXK52859.1"
                     /translation="MKNSSSGHRDSVSAPQCTACFKNLGVEAKYTIYGYLQTEGRANV
                     KTLAKMLKLSQPTVTHHLQQMQQSGLLNRSKVGKEAFYSISETCPVINAPCVMKNITV
                     SNFKEK"
     gene            1682..2416
                     /gene="sufC"
                     /locus_tag="PLF29_02655"
     CDS             1682..2416
                     /gene="sufC"
                     /locus_tag="PLF29_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HXK52860.1"
                     /translation="MLLQINNLRVSIEDKEILKGITLKLGEGEVVALMGPNGSGKSTL
                     AQTLMGNPAYTVTDGNALFDSQKLFSLEANERSNLGLFLSFQYPSEVTGVTISNFLRL
                     IYNKKNNTKISPAKFRTILKEKLSILEMPEDFMSRYLNEGFSGGEKKRMEMLQMLVLE
                     PKLAVLDETDSGLDIDALKIVAKAVSYLRQEKGMAVLIITHYTRVLQYLEPDKVVIMR
                     KGIIVKEGDSSLAQELEDKGYAPFGE"
     gene            2430..3830
                     /gene="sufB"
                     /locus_tag="PLF29_02660"
     CDS             2430..3830
                     /gene="sufB"
                     /locus_tag="PLF29_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HXK52861.1"
                     /translation="MQDIKLNSNYKSKYGFNVKEDFVFKTPKGLNEDIVSQISAMKHE
                     PEWMRELRLKAYSIFLSKPNPTWGGDLSKINYDDIYYYLKPADKEEKSWDDVPQGVKD
                     TFERLGIPQAEREHLAGVKAQFDSEVIYGSLMAELEEQGVIFLGTDDALKKYPEIFEQ
                     YFGTVIPAADNKFAALNTAVWSGGSFVYIPKGIHVKRPLQAYFRINAMNMGQFERTLI
                     IADEGSYAHYVEGCTAPVYSKDSLHSAVVEVIVKPGARFRYTTIQNWSNDVYNLVTKR
                     ARVEKNGVMEWVDGNLGSRLTMKYPSCYLVGEGAHGEVLSIAYAGKHQHQDAGAKMVH
                     LAPNTSSTIISKSVSVLGGRSSYRGLVSVTPTANNAKCRVECDALILDAESASDTYPT
                     MKIDNDTAQIEHEATVSKVGEEKLFYLMSRGLTEAEAMGLVVSGFIEPIVKELPLEYA
                     VELNRLIELEMEGSVG"
     gene            3830..4432
                     /locus_tag="PLF29_02665"
     CDS             3830..4432
                     /locus_tag="PLF29_02665"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HXK52862.1"
                     /translation="MRTILIDKIPSKNIIEVSSSAQYVFDPKLLKGQDVVEVEFLFNT
                     PGVTSEIIALYSCDELSPVKLRTSARHVARSTSCNTVVRAVLRDNSLSDYLGKIKIEK
                     TAIDTSSFLDDAVLVVGKGTHTRSDPILEIEANEVKASHGSTTGRVDESMLYYLESRG
                     LTRNEAEGLIVRGFFESVLGNIKEASVKNYLEPLLGIGEK"
     gene            4429..>5213
                     /locus_tag="PLF29_02670"
     CDS             4429..>5213
                     /locus_tag="PLF29_02670"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXK52863.1"
                     /translation="MKDINTVKNDFPIFKRIYNGKPLVYLDTAATSQKPYSVIDSQTE
                     YYSLHNANVHRGVHFLSDESTDLYEQARIKVQKFLNAESSTEVIFTKGATEGLNRVAM
                     SYAVSALKEGDSILLLDSEHHSNFVPWQEIANILKLKLIFLPLELDGSLDLKKVEKYF
                     TPGVKIISLAHVSNVLGNIYPIKQLAKLAHSRGAVISVDGAQAVGHIPVDVVSLGCDF
                     YSFSAHKMYGPMGVGALYIKKSLHDLIPPFEFGGGMIRTVSLNS"
BASE COUNT         1568 a          947 c         1176 g         1522 t
ORIGIN      
        1 tctcagtgca ggggggatat tgtcgggcca tacactggca taatgtatgc tcctgaagac
       61 ccgactcatc ctaaagagat ctctgcggca actgagttag ctgctctgac ttttagactg
      121 ccgtctcaag aagcctacag aacaggcagt atgacattcc acgatattga tcctcaaaac
      181 ttgctttccg actacgacaa agaacgttat gccatgaaca gtggcggtag tggtgtgaca
      241 accttgcaaa acgtcagctc atatgtgaag gtgccgaatt caacatcata ctccggaatg
      301 gaagctcccg aacttgttga taaaggtgac gatggtaccg ggactacgcc cccacctcca
      361 ggctttgatg gtaaatgcaa cggttctgtt tttgccagta cttttttgaa gaatgctccg
      421 aatttatctc cgacaacatc ggatgtgccg aatgtttccc cccaggttga gggcgtttat
      481 gccgcgcttg aggatgttac aggtttagca tgcgagatat ttgcgggtct tcattataac
      541 gaaacaggcg gcacaaacgc ccctaaccgt tcaattataa gtggtagggt cttaggcgag
      601 aacgaacctg atgttggtaa aacttatccc ccttgcgagg aaggagatac aacatttgaa
      661 tcttgtcttc cgggccttta tgaatcgggc gttgatgccg caagaatttt tattgcgcat
      721 tccgggactt cagcttcaaa tcttgattac tattcttctg tgagagcatt ttctagatat
      781 acatcgccat tttataaaaa ttgtggacac aaacttactc aatacaatga cccatacgta
      841 gtgtatccgg ggtttgcaca gtattgcagc gaccctcttc cttacgaagg ctacgacaac
      901 ctgcctttaa accttattga ttcagaacac agcaacatgg ggcttatgtc ttgcggtttc
      961 cacccggatt ttggcaatta cgtagatatt tgtccaattg aacccgagat taattataat
     1021 aatgaccccc cgacaggtcc cgacggggag gatttaatag gtccttttga tcaatggttt
     1081 cgcatgggat ctgcaacggc ggcatatagg tggtatcaag ctgtaaccag cagccgagcg
     1141 gagagatagt ttttttataa tttcgttact tgaaccgctt gcgtaagcta gtagacatcg
     1201 tcatctaacc tgttgggctt tgcaattcaa ataaattaca ataaaccacc tgcgtagacc
     1261 ggtggtgttt gattgctgcg taaaacccac cggctagaaa ctgattttaa aagactttga
     1321 cttttaggca catatagatt agtatctata tgtgaaaaat tcatcttcag ggcatagaga
     1381 tagtgtttca gcacctcaat gtacagcatg ctttaaaaac ttaggtgtgg aggccaaata
     1441 tactatatat ggctaccttc aaactgaagg tagggcaaat gttaaaacct tagccaaaat
     1501 gctaaagttg tctcagccta cagttacaca ccatctgcaa caaatgcagc aatcgggatt
     1561 actaaataga tccaaggtag gcaaggaggc cttctatagc ataagcgaaa cttgccctgt
     1621 aattaacgcc ccttgtgtta tgaaaaatat tacagtcagt aattttaagg aaaaataaca
     1681 aatgttattg caaataaata atcttagagt ttctattgaa gataaagaaa tattgaaagg
     1741 tattactttg aagcttggtg aaggggaggt cgtagcttta atgggcccca acggctccgg
     1801 caagagtacc ttagctcaaa ccctaatggg aaaccctgct tacactgtaa cagacggaaa
     1861 tgctttgttt gattctcaaa aattgttttc tttggaagct aatgaaaggt cgaatttagg
     1921 tttattttta tcttttcagt atcccagtga ggttacaggc gtaacaatct ctaatttttt
     1981 gcgattaatt tacaataaga aaaataacac taagatttct ccggcaaagt ttagaacgat
     2041 tttaaaagaa aaactatcta ttctagaaat gcctgaagat tttatgtccc gatatcttaa
     2101 tgaaggtttt tctggtggtg agaaaaaaag aatggaaatg ctgcaaatgt tggttcttga
     2161 acctaagctc gctgtgttgg acgaaactga tagcggactc gatattgatg ctttaaaaat
     2221 agtcgcaaag gccgtgagtt atctaaggca ggaaaaggga atggcagttt taataatcac
     2281 ccactacacc cgtgtgttgc agtatttaga gcctgataaa gttgtcatta tgagaaaggg
     2341 cattattgta aaagaaggag atagttcgtt agcgcaggaa ctagaagata aggggtacgc
     2401 tccttttggc gagtaggaat aattaaatta tgcaagatat aaaacttaat tcaaattaca
     2461 aaagcaagta cggctttaat gtaaaagagg attttgtttt caaaacccca aaagggttaa
     2521 atgaagatat agtctctcaa atatctgcaa tgaaacacga acccgaatgg atgcgagagt
     2581 tgcgcttgaa agcatattca atttttttat ctaaaccaaa tcctacttgg ggaggagatt
     2641 tgagcaaaat taattatgat gacatttatt attatctaaa gccggccgat aaagaagaaa
     2701 aaagctggga cgatgttcct caaggagtta aagacacgtt tgaaaggcta ggaattcctc
     2761 aagctgaaag agagcacttg gccggagtaa aagcacagtt tgattctgaa gttatttacg
     2821 gttctttgat ggctgagttg gaggagcagg gagttatatt cctaggcact gatgatgctc
     2881 tgaaaaaata ccctgaaatt tttgaacagt attttggtac cgttatccct gccgcagata
     2941 ataagtttgc cgcgttgaat actgcggttt ggtcgggcgg ttcttttgtt tatattccaa
     3001 aaggcataca tgttaaaaga cctttgcaag catattttag aattaatgct atgaatatgg
     3061 ggcagtttga aagaacttta ataattgccg atgagggaag ttacgcacat tatgttgagg
     3121 gttgtacagc accggtttat tcaaaagatt ctttgcactc tgctgttgtt gaagtaattg
     3181 ttaagccggg cgcgcgtttt agatatacaa ctattcagaa ttggtcgaac gatgtttaca
     3241 atcttgtaac aaagagagct cgtgtagaaa aaaacggagt tatggagtgg gttgatggca
     3301 atttgggatc gcgtcttact atgaaatacc cctcgtgtta tttagtgggc gagggtgctc
     3361 atggagaagt actatccata gcgtatgcag gcaagcatca gcaccaagat gcaggggcca
     3421 aaatggttca tttagctcct aatacctctt ctacaattat ctcaaagtct gttagtgtgc
     3481 ttggcggcag gtccagctat agaggtttgg tgtctgttac tcctacggca aacaatgcaa
     3541 agtgccgagt tgaatgcgac gcgcttatac tagatgcgga atcggcatca gacacctatc
     3601 caacaatgaa gatagataat gatactgctc agatcgaaca cgaggctact gtaagtaagg
     3661 ttggcgaaga aaagcttttt tatcttatga gcaggggttt aactgaagcc gaggcgatgg
     3721 ggcttgttgt ttcgggtttc atcgagccca ttgtaaaaga gcttccgctt gaatacgcgg
     3781 tagagttgaa taggcttatt gagcttgaaa tggaaggatc ggttggttaa tgcgcactat
     3841 attaattgat aaaataccgt ctaaaaatat aattgaggtt tcaagctcgg cgcagtatgt
     3901 ttttgatcct aagctattga aagggcagga tgttgttgaa gtagagtttt tatttaatac
     3961 tcccggagta acttcggaga taatagcttt atatagctgt gacgagcttt ctccagtgaa
     4021 gttgagaact tcagcgagac atgttgcaag atcaacttct tgtaacactg ttgttcgtgc
     4081 agttctccgt gacaattctc tttctgatta tttaggtaaa ataaaaattg aaaaaactgc
     4141 aattgataca agctcttttc ttgacgatgc ggttcttgtc gtgggtaaag gtacgcacac
     4201 tcgctccgac ccgattttag agatagaagc aaatgaagta aaggcatcgc acggctcgac
     4261 taccggcaga gttgacgaga gtatgctgta ctacttggaa agtaggggat tgaccagaaa
     4321 cgaagctgaa ggtttaattg taagaggttt ctttgagtcg gtacttggta acattaaaga
     4381 agcctctgtc aagaattatt tagagccttt gctcggaatt ggggaaaagt gaaagatata
     4441 aacaccgtca aaaatgattt tcctattttt aaaagaatct acaacggtaa gcctttagtt
     4501 tatctggata ctgcagcaac aagtcaaaaa ccctactcag ttattgattc tcaaactgag
     4561 tattactctc tacacaatgc gaatgttcac agaggtgtgc attttttatc agatgagtct
     4621 accgacttat acgagcaagc aagaataaag gttcaaaaat ttttgaatgc agaatcttcc
     4681 acagaagtta tttttactaa aggagctacc gaaggtctaa atagagttgc gatgagctat
     4741 gcggtttcag cactcaagga gggagattcc attttgctgt tggattccga acaccatagc
     4801 aattttgttc catggcagga gatagcaaat atcctaaaac ttaaattaat ttttttaccc
     4861 cttgaattag atggctcgct tgatttaaaa aaggttgaaa aatattttac tccgggagta
     4921 aaaattatct ctctcgctca tgtatcaaat gttcttggca atatttatcc aattaaacaa
     4981 cttgcgaagt tggcacactc tagaggggcg gtaattagtg tcgacggtgc ccaggcagtg
     5041 gggcacatac ctgtagatgt cgtttcttta gggtgcgatt tttatagttt ttctgctcac
     5101 aaaatgtatg gtcccatggg agtgggggct ctttatataa agaagtcgtt gcacgattta
     5161 atacctccgt ttgaatttgg cggcggcatg ataagaacag taagtttgaa ctc
//
LOCUS       DAOSWN010000046         5210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_10624_length_5210_cov_4.775946, whole genome shotgun sequence.
ACCESSION   DAOSWN010000046 DAOSWN010000000
VERSION     DAOSWN010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10624_length_5210_cov_4.775946"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            424..1554
                     /gene="rpsB"
                     /locus_tag="PLF29_02675"
     CDS             424..1554
                     /gene="rpsB"
                     /locus_tag="PLF29_02675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HXK52864.1"
                     /translation="MSKPYKLPKVEDLFSAGVHFGHQVRRWNPAMSEYIYDTRRNVHI
                     IDLQKTHAKLEEACDYLYDLASKGEKIIFVGTKRQAKEMLEVEAKRCGALFVSERWLG
                     GIITNFQEVKKNIDRFLMHIRKMESGEYQSYTKKERLLIERKIEKLRKAIGGLVGLHS
                     SPAALFVIDSKREKTAIREAKASGVKVVALVDTNSNPRDVDYVIPGNDDAIKSVALIL
                     NAVSDAIEAGYKVFEKDAAKIADEKKQNDSDKAKDEVSLDKVVPVVVTSTESPVASSE
                     AGKRLIERVNKGDVSDLDSAKGIEDTVEDTEKSTSKTDREPVAKKVEKKESSREKKVV
                     EKVSKTKAEEKAKKPVKKATAKVAGKKPEKTPVKKSTKKSNK"
     gene            1568..2023
                     /gene="tsf"
                     /locus_tag="PLF29_02680"
     CDS             1568..2023
                     /gene="tsf"
                     /locus_tag="PLF29_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HXK52865.1"
                     /translation="MNLDVQTIKKLREETGAGVLEVKQALEKYDGDYEKSKAELMEKG
                     LAKAAKKSERVTRDGSIVSYIHNGAKVGSLVNVACETDFVAKTDDFQKLCREIAMQAA
                     TEDYESVEDLLNAEYIRDTSKKIKDLIQETIAKVGENIELKAICRFSVR"
     gene            2049..2597
                     /gene="frr"
                     /locus_tag="PLF29_02685"
     CDS             2049..2597
                     /gene="frr"
                     /locus_tag="PLF29_02685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HXK52866.1"
                     /translation="MTNSELKAKLQSSLDFLKEELSYVRTGRATPTMLDEIKVNAYGS
                     MLTLKEVGTLSVLDAQTLSVVPWDKSLLKEIAKAIRESQLKLNPAEQGGSVIVPIPQL
                     TEERRLEFVKIVSVKVEECKVAIRHVRNDAMKDIDQAFARKEFGEDEKFSFKEEVEDT
                     VKDFISKADELGDSKKQDLLAK"
     gene            2619..3785
                     /locus_tag="PLF29_02690"
     CDS             2619..3785
                     /locus_tag="PLF29_02690"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HXK52867.1"
                     /translation="MVAIAIFILIFSLLVIIHEFGHFIVAKKLGVRVDEFGFGMPPRI
                     FGKKVGETIYSINLLPFGGFVQLLGVDENEGPVAEAKKNPRNFLSKKPFQKLAILLGG
                     VIMNLLLAFVLYAVFFSISGYKSSLIPLISDYRFKYGEVSKLSNLIVDVQDNSPAAQE
                     DLVPGEAIIEIDQIPVYNIGDIQAALRNKAGQAVTLLITDARSSEYNVRKVEITPSSN
                     QAGEGIIGVYIGEAVNIDYSRGSSKYLAGPMHSYNMLAYSANVIGTLFQSSLAYKSLE
                     PVSSGFSGPVGIYSVVKGIIAYASQLENPAQRVQVLTLALIDLTALLSLSLALLNAMP
                     FPALDGGRVVFVIFEAITKKQISQAAEMRIHKFGIIFLMGLLALVTVKDLLNIF"
     gene            3845..4198
                     /locus_tag="PLF29_02695"
     CDS             3845..4198
                     /locus_tag="PLF29_02695"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HXK52868.1"
                     /translation="MYDQNCLFCKIVQGQEPCVKVLETEDFLVIENKFPTAPIHLLVL
                     DKQHREKNDTISGAYKNVGYWDSLFTAINETIRYKNLDKTGYKLVNNGAGYNHFEHEH
                     FHILGGSENEPGGTT"
     gene            4290..4484
                     /gene="rpsU"
                     /locus_tag="PLF29_02700"
     CDS             4290..4484
                     /gene="rpsU"
                     /locus_tag="PLF29_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HXK52869.1"
                     /translation="MAKIKVRNNESLEQALRRFNREVLKAGIIREINNRRHYRKPSEI
                     KAEKVKEKNRKIMFEKRKNS"
     gene            4487..4789
                     /locus_tag="PLF29_02705"
     CDS             4487..4789
                     /locus_tag="PLF29_02705"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HXK52870.1"
                     /translation="MLIEQLKNDMKEAMKSKDSFKLSVIRFLLAQIKNKEIELRSEDR
                     SLEESDAVSVLKKQIKQRNQSIELYSKGNRHDLVEKEKLELEVLNAYYEKFAPAVE"
     gene            4796..5203
                     /gene="ybeY"
                     /locus_tag="PLF29_02710"
     CDS             4796..5203
                     /gene="ybeY"
                     /locus_tag="PLF29_02710"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HXK52871.1"
                     /translation="MPAINLPENVYITVKISGDSEIRKLNKKYLKRDCPTDVLSFNLD
                     QETDQGYLLGEIIVNKQQALKQAGKYGNDLEHEIAALVEHGVLHLLGVHHSHDDENSV
                     HGVSTKKATNENTNDGSKQDAREVIKDEGEGSL"
BASE COUNT         1776 a          829 c         1162 g         1443 t
ORIGIN      
        1 atcggcgaag agaggggtga cctgtatgag gcttgcctta cactaactga tttgttccct
       61 ggctttggtc gctcaagtct caggaaaatc ataagtcggt agacattatt tctggctcat
      121 gctactatcc atgctagaag ttgtgagtct aaagattcgc aagctaggtc ccgaaggtcc
      181 ctggcaatag ccttttagta tttaatgaaa aggcacagct ttcgggtgat ggtttattta
      241 taaacccaat tagtaaaagg gaagcagtag gtaagattta atctttgctg taagaatgta
      301 attattaaac acctctcctt tctctttaaa ttattgccaa gtgcagattt ttataaatac
      361 tgctaaatgc taatttgtgt aaaaacgaat ttgcagttta taaatattaa ttttaaggaa
      421 attatgtcaa aaccatataa gttaccaaaa gtcgaagatc ttttttcagc aggggtgcac
      481 tttggccatc aagtccgaag gtggaatcct gcgatgtcgg aatatatcta cgacacaaga
      541 agaaacgtac atattataga tttacaaaaa acccacgcta agctcgagga ggcttgcgat
      601 tatttatacg atttggcgtc taagggagag aaaataattt ttgtcggtac aaaaagacag
      661 gcaaaagaga tgctcgaagt agaggccaaa agatgcggag cattattcgt ttcagagcgg
      721 tggttgggtg gaattataac taatttccaa gaagttaaga agaatataga taggtttctt
      781 atgcacatta gaaaaatgga aagcggagaa tatcaatctt acacaaagaa agaacgcttg
      841 ttgattgaga gaaaaattga aaaattacgc aaagcaattg gcggccttgt gggcttacat
      901 tcgagtcctg cggcactgtt tgttattgat tcaaaaagag aaaaaacagc tataagagaa
      961 gcaaaggcaa gcggtgtaaa agttgtagct cttgttgata caaactcaaa tcctcgagat
     1021 gttgattatg taattccggg taatgacgat gcaataaaat ctgttgcttt aatattaaat
     1081 gcagtctctg atgccattga ggcagggtat aaggtttttg aaaaagatgc cgcaaaaata
     1141 gctgatgaaa aaaagcaaaa tgattcggat aaagcaaaag atgaggtctc tctcgataag
     1201 gttgtgcctg ttgtagttac atcaactgaa tctcctgtgg cttcgtccga agcaggaaag
     1261 aggctaatcg aaagagttaa taaaggcgat gtttcggatc tagacagcgc taaaggcata
     1321 gaagataccg tagaagatac agaaaagtct accagcaaga cagatagaga gcctgttgca
     1381 aagaaagtag aaaaaaaaga gagttcaagg gagaaaaagg ttgtagaaaa agtatcaaag
     1441 actaaagctg aagaaaaggc aaaaaaacct gtcaagaagg ctacagcaaa ggtagcggga
     1501 aaaaagccag aaaaaacccc agtaaaaaag tcaactaaaa aaagtaataa ataaggagaa
     1561 aaagaacatg aatttagatg tacagacaat taaaaaacta cgtgaagaaa ctggagcagg
     1621 tgttttagag gtgaaacaag ctctcgaaaa atacgatggg gactatgaaa agtcaaaagc
     1681 tgagcttatg gaaaagggtc ttgctaaggc ggcaaagaaa tcggaacgtg taacaagaga
     1741 tggatcgatc gtgtcttata tacataatgg cgccaaggtt ggaagtttgg ttaatgttgc
     1801 gtgcgaaacc gattttgttg ctaagacaga cgacttccag aagctttgca gagaaattgc
     1861 aatgcaagca gctactgaag attatgaaag tgtagaagac ttattaaacg ccgaatacat
     1921 aagagataca agcaaaaaaa ttaaagatct aattcaggaa acaatagcaa aagtcggtga
     1981 aaatattgaa ctaaaagcaa tttgcagatt ttctgtcaga taaagttgtt tggtgttatt
     2041 atatttttat gactaattct gaattaaaag ctaaacttca aagttcttta gattttctta
     2101 aagaagaact gtcgtatgtg cgtacaggtc gcgctacgcc aaccatgctt gatgagatta
     2161 aagttaatgc ctacggatca atgctgacct taaaagaggt tggaaccttg agcgtattag
     2221 atgctcagac cttgtctgtt gttccttggg ataaaagctt gctgaaagaa atcgctaaag
     2281 ctattagaga gagccagctt aaacttaatc ccgcagagca gggcggtagt gttatagttc
     2341 ctattcctca gttaaccgaa gagcgcagac ttgaatttgt aaagattgtg tctgtgaaag
     2401 ttgaggaatg caaagttgcc atcagacatg tacgaaatga tgctatgaaa gacatagacc
     2461 aagctttcgc aaggaaagaa tttggggaag acgaaaagtt ctcttttaag gaggaggttg
     2521 aggacacagt taaagatttt attagtaagg ccgacgaact tggggattct aagaaacaag
     2581 acttgcttgc caaatagctc ggtctggaac ttacaaatat ggtagcaata gcgattttta
     2641 tattaatatt ctccttgctg gttataattc acgagtttgg tcattttatt gttgccaaaa
     2701 agttaggagt gcgagttgat gagtttgggt ttggcatgcc tcccagaata tttggtaaaa
     2761 aggttgggga gactatatat tctataaatt tgcttccttt tggaggcttt gtccagctac
     2821 taggcgttga tgagaatgag ggccctgttg cagaggctaa aaaaaaccct cgtaattttt
     2881 tatctaaaaa accttttcaa aaattagcaa tattattagg tggtgtgata atgaacctat
     2941 tactggcttt tgtgctatac gctgttttct tttcgatcag tggttataag tcctccctca
     3001 tccccttaat tagtgattat cgatttaaat acggcgaggt ttcgaaatta agtaacttaa
     3061 ttgtcgatgt gcaggataat tctccggctg cgcaagaaga tcttgttcca ggagaagcta
     3121 ttattgagat agatcaaatt ccggtttata atataggaga tattcaagcc gctctgagaa
     3181 ataaagcggg ccaggctgta accttattaa ttaccgacgc aagatcttcc gaatataatg
     3241 taagaaaagt ggaaattacc ccttcgtcta atcaagcagg ggagggaata ataggtgttt
     3301 acattggaga ggctgtaaat attgattaca gcagagggtc ctcaaaatac ctagcaggcc
     3361 caatgcattc ttataatatg ctcgcatatt cggccaacgt tataggcacg ctttttcaat
     3421 cttctctggc ttataaaagt cttgagcctg tttcttcggg attttccggt cctgtcggta
     3481 tatattccgt tgttaaaggg attattgctt atgcaagtca gcttgagaac cccgctcaaa
     3541 gagtccaagt attaactttg gcactaattg acttaaccgc ccttctatcc ctttctttag
     3601 cacttctaaa cgcgatgccg tttccagcct tagatggagg tcgcgttgtt tttgtaatct
     3661 ttgaggcaat tacaaaaaaa caaattagcc aggcagcaga aatgcgaata cacaagtttg
     3721 gtatcatttt tcttatgggc ttattggcgt tggtaactgt taaagacttg ctcaatatat
     3781 tttagtaagt tttttatctg ttttagattt gtcaaatcta ccccatctgt aatatactag
     3841 gactgtgtac gaccaaaatt gcttattttg taaaattgtg caaggccagg aaccgtgcgt
     3901 taaagtgctt gaaactgaag actttttagt tatagagaat aagtttccta cggctcctat
     3961 tcatttgctg gttttggaca agcagcatcg tgaaaaaaat gatactatat caggtgcgta
     4021 taagaacgtt ggttactggg atagcctgtt tactgccata aatgaaacta taaggtataa
     4081 aaaccttgac aaaacgggct acaaactggt aaacaatggg gctgggtata accattttga
     4141 acacgagcat tttcatattt tgggaggctc cgaaaatgag cccggcggaa ccacctaacc
     4201 ttaggggtta ggtttttgtt gacgctccgg tacgtatgtg cctgagttca gtctaaatta
     4261 aacagataac ggaggtgttt tttaatttta tggcgaaaat taaagtcaga aacaacgagt
     4321 ctttagagca ggcccttagg aggtttaaca gagaagttct taaagcagga attataagag
     4381 agattaataa ccgcagacac tatcgaaagc ctagcgagat caaagcggag aaggttaaag
     4441 agaagaatcg gaagattatg tttgagaaaa gaaagaatag ctaaagatgc ttatagaaca
     4501 attaaaaaat gatatgaaag aagcaatgaa gagcaaagac tctttcaagc tctctgttat
     4561 aaggttttta ttggctcaaa taaagaataa ggaaatagag cttagatccg aagaccgctc
     4621 attggaagag tcggatgctg tttctgtgtt aaaaaagcag ataaagcaac gaaatcaatc
     4681 tattgaactt tactcaaaag gcaacagaca cgatttagta gaaaaagaaa agcttgagct
     4741 ggaagtgctg aacgcttact acgagaagtt tgcacctgca gtagagtagg taagcatgcc
     4801 agcaataaat ttaccagaaa atgtttacat tacagtaaag atatccgggg actcagagat
     4861 aagaaaatta aataaaaaat acctaaaaag agattgtcct acagatgttt tatctttcaa
     4921 tttggatcaa gaaacagacc aaggatattt gttgggtgag attatagtta ataagcagca
     4981 agctttgaaa caagccggga aatatggaaa tgatttagag cacgagattg cggcacttgt
     5041 cgagcatggc gttcttcatc ttctaggggt tcaccactca catgacgacg aaaattctgt
     5101 tcacggtgtt agcacaaaaa aagctactaa cgaaaatact aatgacggtt ccaaacaaga
     5161 cgcaagagaa gttattaaag acgagggaga aggatcttta taatgctggt
//
LOCUS       DAOSWN010000047         5068 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_11070_length_5068_cov_4.810892, whole genome shotgun sequence.
ACCESSION   DAOSWN010000047 DAOSWN010000000
VERSION     DAOSWN010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11070_length_5068_cov_4.810892"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="PLF29_02715"
     CDS             <1..660
                     /locus_tag="PLF29_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXK52872.1"
                     /translation="KEKIEMLDNLRTLLVAGIPIITALDELIEESKGPVHKVLLSVRH
                     DVSEGKSLFSSLDALENTMDKVTLNLIKSAEQSGSLEETLKDIIIFLKRSEEFSSKIK
                     AALLYPTMVVALFLGILLIILYYVIPRISSVFYKLKVDIPITTRVLIIVSNFFVENTV
                     AILSGLLFISVMVSVLYHFKKRSLQTLTFSLPLMEIWQKNLILQDSQGTLRCCYDQEC
                     L"
     gene            651..1022
                     /locus_tag="PLF29_02720"
     CDS             651..1022
                     /locus_tag="PLF29_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXK52873.1"
                     /translation="MPINQALDYSGDVVLQKKTANAIKKSAEAVGEGASLADGLRKSK
                     NVFPTSMIRITEVGEKSGSLEQSMSTLAEKFNKDIDENLKTITVVLEPVLLIIIGIFV
                     GSIMLSIIAPIYKLIGEITPR"
     gene            1024..1521
                     /locus_tag="PLF29_02725"
     CDS             1024..1521
                     /locus_tag="PLF29_02725"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HXK52874.1"
                     /translation="MNHKFIFSPDSGFTLIELMLVIGLFAVLSSFVAVNLARPQVTSS
                     VDKNANTIIADIKKQQLDAMVGKYTSDSTENKGIYFETNSYTLFSGNSYNAEDPNNFT
                     INVEEGTTLTSINLPSNQLVFEHLSGEISGYISGNSSITISHSGGQSITISLNKMGGT
                     NVIKN"
     gene            1505..3358
                     /locus_tag="PLF29_02730"
     CDS             1505..3358
                     /locus_tag="PLF29_02730"
                     /inference="COORDINATES: protein motif:HMM:NF019912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52875.1"
                     /translation="MSLKTNTSIEFGQSLIEVLVSFGILAILLPALLTGFVASREGRP
                     QKENRVAATALVRETYEALRSAREENWYKIASPGIFHPSTDGSSWNLESGPENLGIFT
                     RSIVISDVYRDNTGTIVSAGGTLDPSTKYAEISVSWQTPIASEVFTSTYLTRYLGNTL
                     WEQTTQEQFIQGTFDNTVSKNNAGGEIELEQLPSADWSTPNLAYSYNSPGNTNSLAIF
                     VEGDFAYMGTSTLYIFDISDPYNTTLVGSYSPGGTIYAIVVSNNYAYLATGINSSEIV
                     VVDISNKANPVQASSINLNRNNDLYSIGKFGNYIVAGRDATPAEPELLIFDSSNAPSL
                     SLVGSYEVNNDVLDVYPQGNYVYLSTIEDSAELRIIDISNVNSPTSAGIFDTPSNSDG
                     SGVYFDGNYAYITTSNNGSGAEFYIVDVSSPNNTSLVGSMNIGAYSDTVFVVGDLAFL
                     GTDSVTKEFLVVDISDKSNPVELSSYDTGAGGINSLFVKEGCAFGATDDNSKEFIVLC
                     ADLGGSAYANNGQYTSQTFDAGGSVGFNYFDFISSLPTNTGVSVQIATNTDNSTWNYV
                     GPDGTNNTLYSASGAIPLNSVEGRYFRFKTYLSSDGSNTPILYEARINYSP"
     gene            3355..3873
                     /locus_tag="PLF29_02735"
     CDS             3355..3873
                     /locus_tag="PLF29_02735"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HXK52876.1"
                     /translation="MIIKNRLHIKKGHNQKGMTLVELLLYMGLMSILLVVVTDMLVGI
                     ANIKLESDSVSSVEQDGTYILNRISYDVYRAEAIAMPEVVGSSSGTLELDIGINKYTY
                     STSMDNLLLATSDGAYNLNSNRTTISNVSFTKYGVPGGQETIKIMFTVTSIAEKNSGK
                     ESRNFVTTLGLR"
     gene            3870..4238
                     /locus_tag="PLF29_02740"
     CDS             3870..4238
                     /locus_tag="PLF29_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52877.1"
                     /translation="MKQAGSIMITVLVFSVVAITVLSGAVVVSVINTQGTSDYSQAER
                     LLAISEAGVENAALLMLRDPSYSGNNLSVGNDTVTISVSGTNPLTIESVVQNTDFTRR
                     IQSVMERVGGVLNVTSWDEL"
     gene            4240..>5068
                     /locus_tag="PLF29_02745"
     CDS             4240..>5068
                     /locus_tag="PLF29_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52878.1"
                     /translation="MKKKIFIYLLRNKLAFSSENGEIKSLVFSEEAVKNEELVDAEKF
                     KSEINKFLSDNNLHNLQGPLIVGEECSFSKTLTGDNLEKQIEEFINEVPFEKNKISYK
                     VYNQNENIIIAINNNLCTQIQKELELYNIAITSVVPDMLLPKNKAPLLKILKKSSELK
                     KLSIQRVPEKSKERNGLVLITALLFVLIGVALITMTINSRESESAPIENRELGGLVPT
                     DPILADTIAKSDINIVLIQSDNEEIDYENEIRNLNEMGYSKIQTEVLRDANSEENPQS
                     "
BASE COUNT         1702 a          978 c         1028 g         1360 t
ORIGIN      
        1 aaagagaaaa ttgaaatgct agacaatctt cgcacactgc tagtggcagg aattcctatt
       61 attactgcgc tagatgagtt aattgaagaa tctaaaggtc ctgttcacaa agttttgctt
      121 agtgttcgcc acgatgtgag tgaagggaaa agcctttttt catctctaga tgctcttgaa
      181 aatacaatgg ataaggtaac tctcaacctt ataaaatcgg cagagcagtc aggaagtttg
      241 gaagaaacat tgaaagacat tattatattt ttaaaaagat cagaggagtt ctcttctaaa
      301 atcaaggccg cccttcttta cccaaccatg gtagtagctt tgtttttagg aatactgctg
      361 atcattttat actacgtaat tcccagaatt agcagtgtat tctataaatt aaaggttgat
      421 attcctatca caacaagggt tttaataata gtctcaaact tcttcgtaga aaatacagtt
      481 gcaatacttt ctggcttact tttcatatct gtaatggttt cggttctgta ccactttaaa
      541 aaaagatcat tacagacact taccttttcg ttaccattaa tggaaatttg gcaaaaaaac
      601 ttgatcttgc aagattcaca ggggactttg aggtgctgtt acgatcagga atgcctataa
      661 atcaagcgct cgattattcc ggggatgtgg ttttgcaaaa aaagactgca aatgctatta
      721 aaaaatcggc cgaggcagta ggggaagggg cgagcttggc cgacggcctg agaaaaagta
      781 aaaacgtatt tccaacgtca atgatacgaa tcactgaggt cggcgagaaa agtggttcac
      841 tagagcaaag catgtcaact ttagcagaaa aattcaacaa ggacatagac gagaatctca
      901 aaaccattac agtggttctt gaaccagtgt tattaattat tatcggaata tttgtaggaa
      961 gtattatgct atcgataata gccccaatat acaagctgat aggggagata acgcctagat
     1021 gatatgaatc ataaatttat tttttctcct gactccggat ttacgctaat agagctcatg
     1081 ctagtcatag gactttttgc tgtactatct tcttttgtag ctgtaaacct agcacgcccg
     1141 caagtgacat cttctgttga caaaaacgcc aacacgataa tagcagatat taaaaagcaa
     1201 caacttgacg ccatggtagg gaaatacacc tcagattcaa cagagaacaa aggaatatac
     1261 tttgaaacaa actcttatac attattttct ggaaactcat ataatgccga ggatccgaac
     1321 aactttacta ttaatgtaga ggaaggaaca acacttacaa gcattaacct cccttcaaat
     1381 caattagtgt ttgagcatct tagcggcgag ataagcgggt acatcagtgg caacagctct
     1441 atcacaattt cgcacagcgg agggcaatct ataaccattt cgttaaataa aatgggaggc
     1501 acaaatgtca ttaaaaacta acacttctat agagtttggc caatccttaa tagaggtgtt
     1561 ggtgtctttt gggattctag caatcttact tcctgcacta cttaccggct tcgtagcaag
     1621 tagggaagga aggccgcaaa aggaaaatag agttgctgct acagcacttg tacgcgagac
     1681 ctacgaagct ttgcgcagtg cccgcgaaga aaactggtat aagatagcgt ctccgggaat
     1741 ttttcatcct tcaactgacg gcagttcttg gaacctagaa agcggccccg aaaatctggg
     1801 cattttcaca cgaagcatag ttatttcaga cgtttacaga gacaatacgg gtactatagt
     1861 atctgcagga ggcacactag acccttcaac caaatatgcc gaaatatccg tgagttggca
     1921 aacaccaata gcatccgaag tttttactag cacataccta actagatacc ttggaaatac
     1981 gctgtgggag caaactacac aagagcaatt cattcaagga actttcgaca atacagtatc
     2041 taaaaataat gcgggcggag agattgagct ggagcagctt ccaagcgcag actggagcac
     2101 acctaattta gcatattcgt acaactctcc gggcaacaca aactccctgg caatttttgt
     2161 tgaaggagat tttgcgtaca tgggcactag cacgttgtat atatttgata tttcagatcc
     2221 atacaatact actctagtag gaagttattc tcctggggga acaatatacg caattgttgt
     2281 ttccaataac tatgcgtacc tggcgaccgg cataaactcc agcgagattg ttgtggttga
     2341 catatcaaac aaagcgaatc cggtgcaagc gtcgagcata aatttaaaca gaaataacga
     2401 cctttattct atcgggaagt tcggaaatta catagtcgca ggtcgagacg caactcccgc
     2461 tgaaccggaa cttttaatat ttgattcttc aaacgcacca tctctttctc ttgtaggaag
     2521 ctacgaagta aacaacgacg ttttggatgt ttatccacaa ggaaactatg tatatttgtc
     2581 aacaatagaa gattctgccg agcttagaat tattgacatt tcaaacgtta actctccaac
     2641 atctgcgggc atcttcgaca caccttcaaa ttcagatggg tctggagtat atttcgatgg
     2701 gaattacgca tatataacta catccaataa tggctcgggg gcagaatttt atatagtaga
     2761 tgtgtcttct cccaacaaca cttctcttgt aggatctatg aacataggag cctattcgga
     2821 tacagtgttt gttgttggag atctggcttt tcttggaact gattcggtta ctaaagaatt
     2881 tcttgttgta gacatatcgg ataaatcaaa tcctgtagaa ctgagttctt acgataccgg
     2941 agccggaggt attaattcgc tttttgttaa agagggctgc gctttcggcg caaccgacga
     3001 caattcaaaa gagtttattg tattatgtgc ggatctcggg ggtagcgcct acgcaaacaa
     3061 cggacaatac acttcccaaa catttgatgc aggggggtca gttggtttta actattttga
     3121 ttttatttct agtttgccta caaatactgg cgtctctgtt caaatcgcta ccaacacaga
     3181 taattctacc tggaactatg tggggcccga tggtacaaac aatacacttt actccgcttc
     3241 gggagctatt ccgcttaact cggtggaagg cagatacttt agatttaaaa cctacttaag
     3301 tagcgacggc agcaacacgc caattttata cgaggcaagg ataaattact caccatgatt
     3361 attaaaaaca gattacacat caaaaaagga cacaaccaga aaggaatgac tttggtggaa
     3421 ttacttttat acatgggctt aatgagtata ttgcttgtcg ttgtaacaga catgcttgtt
     3481 ggaatagcga atataaagct ggaatccgat tcggtatcat cagtcgaaca ggatggaaca
     3541 tacatactaa acaggatctc atatgacgta tacagagccg aagccatagc gatgcccgaa
     3601 gtagtcggct cttccagcgg aacgctagaa ctcgatatag gaataaataa atacacctac
     3661 agcacaagca tggataatct tcttttggca acttctgacg gagcatataa cctaaacagc
     3721 aacagaacta ccatttccaa cgtttcattt actaaatacg gcgttccggg aggccaagaa
     3781 acaataaaaa ttatgtttac agtaacaagc atagcagaga aaaatagcgg aaaagaaagt
     3841 agaaattttg taacaacatt aggactaaga tgaaacaagc aggctcgatc atgataacag
     3901 tattagtatt ttctgttgtt gcaattacag ttctgtcggg ggcagttgtt gtatccgtaa
     3961 taaataccca aggaacaagc gactattctc aagctgaaag gcttctagct atcagcgagg
     4021 cgggggtaga aaatgccgca ttgctaatgc tgagggaccc ctcatattca gggaataatt
     4081 tgagtgttgg aaatgacact gttacaatta gtgtatcggg aacaaatcct ctcaccatag
     4141 aatcagtagt acaaaacaca gattttacga gaagaataca gtctgtcatg gaaagggtag
     4201 gaggggtttt aaacgtcact tcctgggacg agttataaaa tgaaaaagaa aatatttatc
     4261 tatcttctaa gaaataagct agctttttct tctgaaaacg gggagataaa atctctggtg
     4321 ttctccgaag aagctgtgaa aaacgaagag ctcgttgatg ctgaaaaatt caagtcggaa
     4381 ataaataagt ttctatccga caataatcta cacaacctac aagggccact gatagtagga
     4441 gaggaatgtt cgttcagcaa aacactgaca ggcgataatc ttgagaaaca gatcgaagaa
     4501 tttataaacg aggtcccctt cgaaaaaaat aaaatatcct acaaagtcta caaccagaat
     4561 gaaaatataa taatagcaat aaacaacaat ctctgtactc agattcaaaa agaactagaa
     4621 ctttacaaca ttgctataac aagtgttgtt ccagacatgc tcttgccaaa aaataaagcg
     4681 ccgttgctaa aaatattaaa aaaaagcagt gagttgaaaa aattgtctat acaaagggtc
     4741 ccagaaaaat ctaaagagag aaacgggctt gtacttatta cggcccttct tttcgtttta
     4801 ataggcgttg ccttgatcac aatgaccata aactcacgag aatcggaatc ggcccccata
     4861 gagaatagag aattaggggg tttagtaccc acagatccaa tactagctga cactatagct
     4921 aaaagtgaca taaatatagt tcttatacaa agcgacaacg aagaaataga ctacgagaat
     4981 gagatccgaa acttgaacga aatgggatat tctaaaattc aaacagaagt gcttcgtgat
     5041 gcaaattctg aagaaaaccc acagagcc
//
LOCUS       DAOSWN010000048         5038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_11168_length_5038_cov_4.447321, whole genome shotgun sequence.
ACCESSION   DAOSWN010000048 DAOSWN010000000
VERSION     DAOSWN010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11168_length_5038_cov_4.447321"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1451)
                     /locus_tag="PLF29_02750"
     CDS             complement(105..1451)
                     /locus_tag="PLF29_02750"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HXK52879.1"
                     /translation="MDAVQKITSLCKRRGFVFQSSEIYGGWEAAYDFGPLGAEMLRNI
                     RNLWWKEFVHKQPNIVGLDSAIISHPRVWEASGHVESFADAMVEDIKNNKRYRADHII
                     GEYFEKKNGEEFNADGMSLEELDILIAENNILSPDGNKLSKARKFNSLVEVSIGTLED
                     AKATAYLRGETCQPIFYNYALVRDSMRMKIPFGIAQIGKAFRNEIKVGPFFFRTREFE
                     QMEMELYVHPSDAENQMAKLKSWAIDWLVSLGIPEDKLRLRDQDFKERAHYNKIATDV
                     EYRFPFGWKEFQGLHYRSDWDLSRHAEFSGTNLDYTDPVTGEKYVPHVVEYSIGLNRL
                     MLVLLFDAYVEEEERVVLKLHPDIAPYRYAVFPLLKNKPELVDKARLIYNTLVTKGYN
                     VAWDDRGNIGKRYYSQDEIGTPYCITVDFQTLEDETVTIRDRDTASQRRLRIDELG"
     gene            1770..2093
                     /locus_tag="PLF29_02755"
     CDS             1770..2093
                     /locus_tag="PLF29_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52880.1"
                     /translation="MYEALNQPIEVLISFTKNGPIPTSFIWKKKEYYIQELNFIYKEK
                     RGNETIHYFMVSDDSAAYKLCFETESLRWLLMEVYDTLQPENGVSAVKERITITYARQ
                     NNITC"
     gene            2065..3366
                     /locus_tag="PLF29_02760"
     CDS             2065..3366
                     /locus_tag="PLF29_02760"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HXK52881.1"
                     /translation="MRDRIILHVDYDSFFASVEQQANPFLRNKVVGVTSANSNKGIIV
                     APSIQAKRVGIKTGMPVFKARALYKNIILVKSDFVKYTHVQKESLKIFAKYTDKIEPF
                     SIDEAFLDITQTCKFFGTPENVALEIKSELRKTIGTLITCSIGIGPNKSMAKIASEIN
                     KPNGIFKLTRNNMEEVYKEIELTDVCGIGKATAKKLRDLDINNLEDLAKTNYAILKLN
                     FGNSLANFLTDIVKGTESSGVSSIDFKRVPKSMGHQHTLSSSTKDKLIIRATIKGLTE
                     LLARRLRKYKMRCTGLSLYIKDERGLGREAKQSFTKGVDSGQILFEILHQLYLGLNVE
                     QRVRFIGIRAYKLESARYSVLGLFEEETRNELPKPNSNRNTSARSQENTLQQIKYDRK
                     EALSKLLDSLNNVWGTKTIFFGTEIHAGEVQGKTSSFISYD"
     gene            complement(3363..4085)
                     /gene="recO"
                     /locus_tag="PLF29_02765"
     CDS             complement(3363..4085)
                     /gene="recO"
                     /locus_tag="PLF29_02765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HXK52882.1"
                     /translation="MKKHNLTCIVLKSFNYKDTDKIYTLFSEERGKISVTAKGVRKIT
                     SKRAGSLDTLNLIHASISEAKSGQLYLSELEVIDPFKNIKNAYDLSNKAYYLIEIVDK
                     FLFDQQEHRNLFLALLRSLSLLDKNPSTSDFSVCYFELRIMEYLGYGISLDKCVVCMK
                     PYSDDWDVYKLNTSSGGLICGSCSVSGLHIDLTKARQLHSLAKGKYLSDVSYRAVGDL
                     LKTYVRSVLDQNPKTLQIFGNV"
     gene            complement(4166..>5038)
                     /locus_tag="PLF29_02770"
     CDS             complement(4166..>5038)
                     /locus_tag="PLF29_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52883.1"
                     /translation="HSPNVKPVDGVITINTQVLEELLQVTGPVTLNGITYDSETVTLE
                     LEKIASLALREQANRKKILGDLMEAMLKNVFESDKNLWPQLVDKGLDLAARKHIKAFM
                     FDPQAQALLEKYNYAGRIADPIPGSGDYAHLVSTNLGGGKTNTWFVNKSVEHSLTREG
                     DRYVRTVAATFTYGDKGPEYDVFKTRYQDWLRLYVPLGSELISIENSDDPAGTGEERN
                     KTYFHGYVTLSPGETKTVTFKYYLPEDVVKGDTYNLYLQKQSGINSEPHTITIGNKSE
                     TVELEKDQLYSTKL"
BASE COUNT         1578 a         1013 c          931 g         1516 t
ORIGIN      
        1 aaaaacaggc agcaaaaaaa ggtttcttat aatgttatag gttaattata cgcctaatga
       61 agcgttttgt aaatattata ggtcctttac gggggggggg gaggttaccc gagctcgtcg
      121 attcttaagc gtctttgtga agctgtatcg cgatctctga tagtcacagt ttcatcctct
      181 aaggtctgaa aatcgactgt aatgcaatag ggagtcccta tttcgtcttg gctatagtac
      241 ctttttccaa tatttcctcg atcatcccaa gccacattat agcctttggt aactagagtg
      301 ttgtatatta gccgagcttt atccacaagc tcgggtttat tctttaaaag cgggaaaaca
      361 gcatacctat agggtgctat atctgggtgc agtttcagaa ctactcgttc ttcttcctca
      421 acataagcat caaacaacaa caccaacata agtctattaa gccctatgga gtactcaacg
      481 acatggggaa catatttctc ccctgttaca gggtcggtgt aatctagatt agttcccgaa
      541 aattccgcat ggcgacttaa gtcccagtcg cttctgtagt gaaggccttg aaattctttc
      601 caaccaaaag gaaatctata ctcaacgtct gtagctatct tattgtaatg agcccgctcc
      661 ttaaagtctt ggtcgcgcag cctgagctta tcttcaggaa ttcccaaact tactaaccag
      721 tctatagccc aagatttcaa tttcgccatt tgattttcgg catctgaagg atgcacatac
      781 agttccattt ccatttgttc aaattcccgc gttctaaaaa agaaagggcc gacctttatc
      841 tcatttctaa acgccttgcc tatttgagct attccaaaag gtattttcat tctcatggaa
      901 tctcgaacta aagcgtagtt ataaaaaatg ggctgacatg tttctcctct taaataagcg
      961 gttgctttag cgtcttctag tgtacctatt gagacctcaa ctaaactatt gaattttctt
     1021 gcttttgaaa gcttatttcc atcggggctt aaaatgttat tttcagcaat taaaatatcc
     1081 agctcctcaa gactcatgcc gtcagcatta aactcctctc cgtttttttt ctcaaagtat
     1141 tccccaataa tgtggtcggc tcgatatctt ttattgtttt ttatatcttc aaccattgca
     1201 tcggcaaagg attcaacgtg cccgctagcc tcccataccc tcgggtgaga tataattgca
     1261 ctatctagcc ccactatgtt tggttgttta tgcacaaact ctttccacca taaatttctg
     1321 atgtttctta gcatttcagc tcctagagga ccaaagtcgt aagcggcttc ccacccacca
     1381 tagatttctg aggattgaaa aacaaaacct cttcgtttgc ataaacttgt tattttttga
     1441 accgcgtcca tggaactatc ctaccataac cataagcagt attaaatccc attttaaata
     1501 caatcagcag cgggttttac ccatctcaaa aggtgtttga gtactttgtt gattttttga
     1561 gacccggacc cataagataa gagtaaaaaa cgtgctttta caaaataatc aactgccgct
     1621 agctcactca ggcgggttac tgtagtctca ttacgcttca aagccaacat ttcgggcaaa
     1681 gtacatcttc tagcctatgc aagtggtttt gaataactaa actataaaag cacttgacat
     1741 aatagccccc ttcgggttat attcggtata tgtacgaggc gctaaatcaa cctatagaag
     1801 tattgatttc ctttaccaaa aatggcccca taccgacaag tttcatatgg aaaaagaagg
     1861 aatactacat ccaagaatta aattttatat ataaagaaaa aagaggaaat gaaacaattc
     1921 actactttat ggtctctgat gactctgcgg cgtataagct atgttttgaa accgagagcc
     1981 tgagatggtt acttatggag gtatatgata cgctgcaacc ggagaacggc gtttctgcag
     2041 taaaagaaag aataactata acttatgcga gacagaataa tattacatgt tgattatgac
     2101 tctttcttcg cttccgtaga gcaacaggca aatccgtttc taagaaataa agttgttgga
     2161 gtaacaagtg caaattcgaa caagggcata attgttgccc cctcaataca agctaaaaga
     2221 gttggcatca aaacaggcat gcctgttttt aaagcacgtg ctttatataa aaacataatc
     2281 ttggttaaaa gtgattttgt aaaatatacg catgttcaaa aagaaagctt aaaaattttt
     2341 gctaaatata ccgataagat agagccattt agtatagatg aggcatttct tgatataacg
     2401 caaacctgca aatttttcgg cacgcccgaa aacgtagcac tagagattaa gtcagaactt
     2461 agaaaaacta tagggacgct tattacatgc tccataggca tagggccaaa taaaagcatg
     2521 gcaaaaatag cctcagaaat aaataaacct aatgggattt ttaaactaac tagaaataac
     2581 atggaagaag tttataaaga aattgaactt acagatgttt gtgggatagg taaggcaaca
     2641 gccaaaaaac taagggattt agatataaat aatcttgaag atctagctaa aacaaactac
     2701 gcgattctta aattaaattt tggaaactcc ttagcaaatt ttttaacgga cattgttaag
     2761 ggcacagaga gctcgggggt cagttcaatt gattttaaac gagtacctaa atcgatgggc
     2821 caccaacata cgctatcttc cagcacaaaa gataaactga ttattagggc aacaataaaa
     2881 ggcctaaccg agcttttggc acgacgttta agaaaataca aaatgagatg cacaggttta
     2941 agcctctata tcaaagatga aagggggtta gggcgcgaag cgaaacaaag ctttacaaaa
     3001 ggagtggatt ccggacaaat tttatttgaa attctgcacc agctgtactt gggcctcaac
     3061 gttgaacaaa gagtgaggtt tataggcatt cgagcctaca aactggagtc tgcaaggtat
     3121 tccgtgctgg gcctgttcga agaagaaaca aggaatgagt tgccaaagcc aaactccaac
     3181 agaaacacta gcgcaaggag tcaggaaaat actttacagc aaattaaata cgatagaaag
     3241 gaggctctaa gtaaattgct ggacagcctg aacaatgtct ggggtactaa aactattttt
     3301 tttggcactg aaattcatgc tggcgaagta caaggcaaaa caagtagttt tatcagctac
     3361 gattaaacat taccaaatat ctgaagggtt tttggatttt gatcaagtac ggatcttaca
     3421 taagttttta gaagatcgcc aactgcccta taagaaacat cagataaata ctttcccttt
     3481 gcaagagagt ggagttggcg agccttagtg agatctatat gtaagccgga aacactacag
     3541 ctaccgcaaa ttaaacctcc ggacgaggtg tttaatttat atacgtccca atcatccgag
     3601 tagggcttca tgcaaacgac gcatttgtcc aaagaaatac catagcctaa atattccata
     3661 atccggagtt caaagtaaca tacactaaaa tctgacgttg agggattttt atctaaaaga
     3721 gacagggatc tcagcaaagc caaaaagaga tttcgatgtt cttgctgatc aaataaaaat
     3781 ttatctacaa tctcaataag gtagtaggcc ttattagata gatcgtatgc atttttaata
     3841 ttcttaaacg gatcaataac ctcaagctca ctaaggtaca gctgtccgct tttagcttcc
     3901 gatatagatg cgtgaattaa atttaaagtg tctaaacttc ctgctctttt ggatgttatt
     3961 tttcgaaccc cctttgcagt aacagagatt ttcccccgct cctctgaaaa gagggtatat
     4021 attttatcgg tgtctttata gttaaatgat tttagaacaa tacaagttag gttatgtttt
     4081 ttcattggaa tggattgggt ttggggggct ttttgtttta caaaaagccc ccacaacttt
     4141 caaatcataa tcgattaaac ccaagctata actttgttga atatagttgg tctttctcaa
     4201 gctctactgt ttctgactta tttccaatag ttatagtgtg aggttctgaa tttatacctg
     4261 actgcttctg tagatataaa ttatacgtgt ccccttttac aacatcctcg ggaagatagt
     4321 atttaaatgt aacggtctta gtttctcccg gagacagtgt cacgtaacca tggaaatatg
     4381 ttttattcct ctcttctcct gtaccagcgg gatcatccga attttcaata ctaattagtt
     4441 ctgaacctaa tggaacatac aatctaagcc aatcttgata ccttgtttta aacacatcgt
     4501 attcaggacc cttatcgcca taggtgaatg ttgcggccac agttctaaca tacctatcac
     4561 cttctctggt taaagaatgc tcaacggatt tatttacaaa ccaagtgttt gttttccccc
     4621 cgccaagatt cgtggaaaca agatgggcat aatctccaga tccaggaata ggatcagcta
     4681 ttctgcctgc atagttgtac ttttccaaca atgcctgtgc ctgcgggtcg aacataaaag
     4741 cttttatgtg ctttcttgct gcaagatcaa gccccttatc tacaagctga ggccacaaat
     4801 tcttatctga ttcaaaaacg ttctttaaca tggcttccat caaatctcca agaattttct
     4861 ttcggtttgc ctgctctctc aaagcaaggc ttgctatttt ctctaactct aacgtgactg
     4921 tttcggagtc ataagtaata ccatttagtg ttaccggccc tgttacttga agcaattctt
     4981 caagaacttg ggtattaatt gttattacac catcaacagg tttaacgttt ggagaatg
//
LOCUS       DAOSWN010000049         4978 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_11372_length_4978_cov_3.995937, whole genome shotgun sequence.
ACCESSION   DAOSWN010000049 DAOSWN010000000
VERSION     DAOSWN010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4978
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11372_length_4978_cov_3.995937"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PLF29_02775"
     CDS             complement(<1..314)
                     /locus_tag="PLF29_02775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXK52884.1"
                     /translation="MSLLTARKRVRMRSSSNRVLGFTLVELLMVISIIGILASVILVA
                     YNPYKFLQNSYYRQSKADLLSIRKGLMLYAIKYGEYPPDVWRDLPSGIEEFIQHGSQW
                     PKG"
     gene            complement(316..1365)
                     /locus_tag="PLF29_02780"
     CDS             complement(316..1365)
                     /locus_tag="PLF29_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52885.1"
                     /translation="MPENLNTNRIESEDLHSGQVTDTLGQNSKKSRSKAKPILIVTSL
                     VLFLGIVAAVFFAYSFNKSYSKHVGMLKDKSQSQSKQLDSLSTSFSIVVNTMKDSNED
                     DLQSSISLIQNTEKENSGEVLALEDSPEMKKLRKLSEEYRSIIETLGKIEATNKDIEN
                     LLVEKPYVKYLNYKNPQLDTQVIKDTNEFTKNAGIIMSYFKDQTVLSIEESTVIYDLV
                     ADVVSAFYSDFDEFYVSSIEAKLDKLDSTQNKLENLNTDNLPDSIKEGHRAHVEELDA
                     GVKDLRVLVQAMRDKDYYFLLSFAQSLDTATSSVVASEEVALSEAITFWQENETIRSV
                     PGLVKSWENFGTSIK"
     gene            1425..2750
                     /locus_tag="PLF29_02785"
     CDS             1425..2750
                     /locus_tag="PLF29_02785"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXK52886.1"
                     /translation="MDYKNYTKQLEPLISFKSISTDPAYKSEIIKAVKWIEKYLNDSG
                     FTTELWQGEISNPVVFGTIEVDPAFETVLVYGHYDVQPANISDGWEKDPFELFEKGDR
                     LIARGVVDNKGQFFIHMYTVCELIKTKRLKYNVKFLIEGNEETSNTELASQLQKNKDK
                     LTADYIMVSDGELIGTNPVIEYSLRGGFNCKITYTTAKNNLHSGLYGGAVPNSAQELS
                     AFLNKVFTSGKVAFDWFYKDVDEISQQQQEQNTKAVENLESILDNIGVKKLTTESELD
                     LLSQVGLRPTIQITGFKAGYTEEGYANIVPAQAEVRLNFRTVMFQNTEEIKNKFADFV
                     KQNTPAYIKYELEFTNAYNPIKIDTSSQIFSKVRTVLETAFGKPALSKPVGGGIPVVN
                     DFKSVLGKDTLLVSLGNDDCNMHGVNENFNIKLLEKGLTFSENFFTKKD"
     gene            2913..3110
                     /locus_tag="PLF29_02790"
     CDS             2913..3110
                     /locus_tag="PLF29_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52887.1"
                     /translation="MILVLTPEQIRKNLLRDASWVPAEDASDMDWELYQRVYDDMIES
                     GELDEPDDDMDEGLYSDDDDY"
     gene            complement(3306..4403)
                     /locus_tag="PLF29_02795"
     CDS             complement(3306..4403)
                     /locus_tag="PLF29_02795"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HXK52888.1"
                     /translation="MKELEIKRKSYKTPIYLPDATLGVVRGLDSKDLLGAGVKGVVVN
                     TYHLHHRPGLEHLSEAGGIKAFMGFDGLVASDSGGWQIFSLIHRHGLPGKISDEGVKF
                     ADKDGGKKMKLFTPEDSIKMQFELKSDIVVCLDDFTPPDADRVTIEKSVNRTVLWAQR
                     SKIEYERQLNIRGIERANAPVLMAVVQGGYDKSLRLQCCNDLSSIGFDMYGFGGYVVN
                     EDGLDLEISEYLASIIPSGAYKFALGVGTPWQIAMCYKFGWDIFDCTLPTRDARHGRL
                     YVYTGDYSNPSDLLTKGLHSHISITSGKYFKDHFPISDSCDCLTCSNYTRSYLHHLFK
                     VKDVSAYRLATIHNLRTYTRLIEALVASDIR"
     gene            4508..4906
                     /locus_tag="PLF29_02800"
     CDS             4508..4906
                     /locus_tag="PLF29_02800"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXK52889.1"
                     /translation="MTLNLVPSSFWDLSTSKFPSIWDEDFTAASGVSIYEDDKKVYVE
                     AAVPGINPEEVEVTLDKNVLWIRGESKTEDKSKKYYKKATSNFSYRVSVPSNVDLKND
                     PEAVCKNGVMTVSFNKKEEEQPKKIAIKKS"
BASE COUNT         1629 a          993 c          947 g         1409 t
ORIGIN      
        1 cccttgggcc attgcgagcc atgctgaata aactcctcga ttccgctggg aagatctcgc
       61 caaacatccg gagggtattc tccgtactta atggcataga gcataagacc ttttcttata
      121 gataacagat cggcctttga ttgcctgtaa taagagtttt gtagaaattt gtagggatta
      181 taggcaacca gtatcactga ggctaagatg ccgataatcg aaatgaccat aagcaactca
      241 actaacgtga atccaagtac gcggtttgaa ctagagcgca ttctgactct cttgcgggca
      301 gttaataaag acacattatt ttatgctagt gccaaaattt tcccaagatt ttactaagcc
      361 aggaaccgat cttatggtct cgttttcctg ccaaaaagtt attgcctcag acagagcaac
      421 ttcttctgaa gctactaccg aacttgttgc agtgtctaaa ctttgagcaa aacttaaaag
      481 aaaataataa tctttatctc tcattgcttg tactaatact ctaaggtctt taactcctgc
      541 atcaagttct tctacatgtg ctctgtgccc ttccttgatt gaatcgggaa gattatctgt
      601 gtttaaattt tctaacttgt tttgagttga atccaactta tctagctttg cctctatgct
      661 tgaaacgtaa aattcatcaa agtcgctata aaaagcgctt actacatctg caactaaatc
      721 ataaattacc gtagattcct ctatgctaag cacggtctga tctttaaaat acgacattat
      781 tattccggca ttttttgtaa attcatttgt atcttttatt acttgagtat ccagctgagg
      841 gttcttatag ttcaggtact tcacgtatgg tttttcaacc agcaaatttt ctatgtcttt
      901 gttagtagct tcaattttac cgagagtctc gattatcgac ctatactctt ccgataattt
      961 tcttagtttt ttcatctcag gggaatcttc aagtgccaat acctctcctg agttttcttt
     1021 ctccgtgttc tgtattaaag aaatgctact ttgaagatcg tcttcattag aatctttcat
     1081 ggtgttaaca actattgaaa atgaagttga aaggctgtcc agctgctttg attgcgactg
     1141 agacttatct tttaacatgc ccacatgctt gctgtaagac ttattaaagc tatatgcaaa
     1201 aaaaactgca gccacaattc ctaaaaatag gaccagagaa gtaacaatta gaattggctt
     1261 agctttgcta cgggacttct ttgagttctg gccgagagta tctgtaactt ggccggaatg
     1321 taaatcttca gactcgattc tattagtatt taaattttca ggcataaata aataataaag
     1381 tacaaaaatg acactgtaaa gtaaaaactg ctataatcca aaacatggac tacaaaaatt
     1441 acactaaaca acttgaacca ttaatttcat ttaaaagcat ctcaacagat cccgcctata
     1501 aaagcgagat cataaaagca gttaaatgga tagaaaaata ccttaatgat tcgggattca
     1561 ctacagaact ctggcaagga gaaatatcta atccggtggt ttttggcaca attgaagttg
     1621 atcccgcatt tgaaactgta ctggtatacg gacactacga tgttcagcct gcaaatatta
     1681 gcgatggttg ggaaaaagac ccgtttgaat tgtttgaaaa aggcgacagg ctgattgcaa
     1741 ggggagtggt agataataaa gggcaatttt ttattcatat gtatacggta tgtgagttga
     1801 taaaaacaaa gcggcttaaa tacaatgtaa aatttttgat agaaggcaat gaagaaactt
     1861 caaacacaga acttgcttcg cagttacaga aaaacaaaga caaactaaca gcggactaca
     1921 taatggtatc tgatggcgaa cttataggaa caaacccggt aattgaatat tctttaagag
     1981 gcgggtttaa ttgtaaaatc acctacacta cggcaaaaaa caacctgcac tctggtttat
     2041 acggaggggc tgttccaaat tcggctcaag aattgtctgc atttttaaat aaagtattca
     2101 caagcggcaa agtggctttt gattggttct acaaagacgt tgacgagata agccagcagc
     2161 aacaagaaca aaatactaag gcggttgaga atctggaaag catcttggac aatataggcg
     2221 taaaaaagct tactactgaa agtgaactgg atttgctttc acaggtaggt ctaagaccaa
     2281 caattcaaat tacaggcttt aaagcaggct acacagaaga aggctacgca aatatagtcc
     2341 cggcacaagc ggaggtaaga ttaaactttc gaaccgtaat gtttcaaaat acggaagaaa
     2401 taaaaaataa atttgctgat tttgtgaaac aaaacacacc cgcttacata aaatatgagc
     2461 tggaatttac aaatgcctat aatccaataa aaatagacac ttcaagccaa atcttctcta
     2521 aagtaagaac tgttctagag actgcttttg gaaaacctgc gctatctaaa ccagttgggg
     2581 gaggaattcc tgtagtaaat gattttaaaa gtgtacttgg aaaagacaca cttcttgtat
     2641 cccttgggaa tgacgactgc aacatgcacg gagtaaacga aaactttaat attaagctct
     2701 tggaaaaagg tttaactttc agtgaaaact tcttcactaa aaaggattga tttagttaac
     2761 ttttaatgca aaaattacca aagaaatgtg ccttttaagg caattttcta caatcaagcc
     2821 tgaatataga atcagggaag acggtaggat caaataataa atgctttgta atacttttat
     2881 aaatcaaaaa gaattactga ctttcagagg gggtgatact tgtgttaact cctgaacaaa
     2941 tcagaaaaaa tttgttaaga gacgcatcat gggttcccgc tgaagatgca agtgacatgg
     3001 attgggaact gtatcaaagg gtgtacgacg atatgataga atccggcgaa ttggacgaac
     3061 ctgacgatga tatggacgaa ggcctgtaca gcgatgacga tgactactaa ttgtaccgtt
     3121 actgctctta gggcgatcgg cgcgaaacac tgggcgtaaa agtctggcaa agccgaccta
     3181 ctaaagagtg gtggaggggg atgcttcggg ttgaggcccc caaagctcgc ttttgaaggt
     3241 aaaggccccg ctacgcgggg ccccaacgac ataaaccata aaaaacaaga tactaaatca
     3301 ctaaactatc taatatccga tgctaccaaa gcctctatca gccgagtgta cgtacgaaga
     3361 ttgtgaattg ttgccagtct gtaagcggac acatctttaa ccttaaaaag gtggtgcagg
     3421 taagatctgg tgtaattcga acatgttaaa caatcgcagc tatcgctaat aggaaaatgg
     3481 tccttaaaat attttccact agttatagaa atgtgagaat gcaagccctt agtcagtaaa
     3541 tctgaagggt ttgaatagtc tcctgtatat acatagagcc ttccatgccg tgcatctctt
     3601 gttggcaaag tgcaatcaaa tatatcccaa ccgaatttat agcacatagc aatttgccag
     3661 ggcgttccca ccccaagagc aaatttgtac gcgcctgaag gtataataga agctaaatat
     3721 tcagatattt cgaggtccag cccatcttca ttcactacgt aaccgccaaa accgtacata
     3781 tcaaacccta tggatgaaag atcattgcaa cattgcaatc ttaaagattt atcgtatcct
     3841 ccttgaacta ctgccatcaa caccggggca tttgcacgct ctatgcctcg tatattcaac
     3901 tgccgttcgt attcgatctt acttctttga gcccacaaaa cagtcctatt tacagacttt
     3961 tctatcgtta cacgatcagc atccggcggt gtgaaatcat caagacacac cacaatatcc
     4021 gactttaatt caaactgcat ttttatcgaa tcctctggtg taaacaactt cattttcttg
     4081 ccaccgtctt tatctgcaaa tttcacgccc tcgtctgaaa tttttcctgg caatccatgt
     4141 ctgtgtatta gagaaaaaat ttgccaacca cctgaatctg atgctaccaa accatcaaag
     4201 cccataaatg ctttaatccc gccggcctcg gaaagatgtt ctagcccagg gcgatggtga
     4261 agatgatatg tattaactac aactccttta actcctgcac caagaagatc ttttgaatcc
     4321 agccccctaa ctactccgag ggtagcgtcc ggtaaatata taggagtttt atagcttttt
     4381 cttttgattt caagctcctt catgttgttg ataatagcac atccgaaatt tgcctactca
     4441 aaacttgaca gacaattagc actcgttgta taaaagtgat aagtatatat tttccaagga
     4501 ggcaaatatg accttaaatt tagttcccag ctcattttgg gatctttcaa catcaaagtt
     4561 tccctcaatt tgggatgaag atttcacagc agcgtccggc gtatctattt acgaagacga
     4621 taaaaaagta tacgtagaag ccgcggtacc gggtataaat cccgaagaag tagaggttac
     4681 tttggacaaa aacgtactat ggatcagagg agagtcgaag acagaagata aatctaaaaa
     4741 gtattacaaa aaagctacct ctaacttttc gtacagagtt tcggtaccaa gcaatgtaga
     4801 tcttaaaaac gatcccgaag cagtatgtaa gaacggagta atgacagtat cattcaataa
     4861 aaaggaggag gaacaaccaa aaaagattgc tataaagaaa tcttagtgtt tggtttagcc
     4921 aagaattctg aggttggggc cacggcccca acctcaaccg ccccccccat acccacac
//
LOCUS       DAOSWN010000050         4813 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_11921_length_4813_cov_4.988441, whole genome shotgun sequence.
ACCESSION   DAOSWN010000050 DAOSWN010000000
VERSION     DAOSWN010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4813
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11921_length_4813_cov_4.988441"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3040
                     /locus_tag="PLF29_02805"
     CDS             <1..3040
                     /locus_tag="PLF29_02805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK52890.1"
                     /translation="LPPYISILCIYTTLAFAIYLLRNKENVAEKNIKNYKHLVYLIFF
                     VFSWQTSEFMAVNLSLQQEQSAILWKITLSLSITAALSTLYFIVNFLQIKNRRILYIS
                     TLLVFIETINVLFTQNVISATYKTSNGQTLFEKGSISAGIGHNILLVALLLLSLGFIV
                     NALKINSVKRASIRFLMFGVISVILPQILFGVIFPIFNLPTVRIGQISIIFVVFFFYK
                     AVSEKSSIILSKQYISIKTKFIFVFALISLGAVILTAIPTSTLLKNLSERTQAKNISI
                     QVENASKDINTFLDEYASDALFLSNLGTVRRLEIDQIQNEFFSLAESNSDYMQVRLID
                     INDQEIVRVDSDEKSTRIIDEDKLQNKVDRYYFTDSIVLNQKEVYMSKIDLNREGSPP
                     QIEIPINPTVRFATPFYSPDGEKLGIVILNVHANKLLESLNHNNDVRFTDESGYFIAH
                     PNSELEWGSPADFDTGYNFSLELSSQDIEGMRSEQTYYSKGQQGLVYANKLTLYNNTF
                     FVYTALDYSVILQDINNLQISLLSLGALYSILSLVFAEIASGFVVKNLIEIRKTVERI
                     NKTKEIEYLTINSHDEIGEISILLNEWARKINLTNNEIQKTVDNQVRQIKNNEDKLKE
                     QQKALMNVLEDVQEEKQKAEQHSTELIKFKLAVENSSDQIVITDPEGYILYANPILEK
                     ITGFSVEESVGKKAGSRELWGGIMEPEFYEVMWTKIKKEKVLFKGQIKNRKKSGEEYT
                     ASVTIVPILNDKGETIFFLGVERDITKEAEVDKMKTEFISLASHQLRTPLSAIKWFLE
                     MLIDGDLGTLNEEQQKTLLDVNTSNERMISLVNSLLNVSRIESGRIIIDPEPTELKDL
                     IDGVLMELTPQIKEKEISTIVSIGDNMEKISIDPKLIRNVYLNLISNAVKYTSKNGEI
                     QIFVSHNDTEVISAISDDGIGIPKEQQDRIFQKFFRADNVIKVETEGNGLGLYLVKAI
                     VESSGGKVWFSSTEGEGTTFWFSLPKKGSMKKKGEVSIDS"
     gene            3053..3472
                     /locus_tag="PLF29_02810"
     CDS             3053..3472
                     /locus_tag="PLF29_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXK52891.1"
                     /translation="MNTSSKQKPKNLSTILVVEDEALLLTAISAKLNKLGIEAVTVTS
                     GAQALDYLENLQQYSALPDAIWLDYHLKDMDGLQFMSAIRKNPELSKIPVIIVSNSAS
                     NDKVESMLALGAKKYLLKAEHRLDEIVNIFGELVENE"
     gene            3465..3941
                     /locus_tag="PLF29_02815"
     CDS             3465..3941
                     /locus_tag="PLF29_02815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXK52892.1"
                     /translation="MNNITDSSFINKGSPSLNQRPDQSVPTNKDTKGASNNKHVLIAE
                     DDKFLGNAYRVKLEKSGYQVTLARDGVEAMEELSKGSDFCLIILDLIMPKKDGFATLE
                     EIKQQPSLKDIPILIASNLGQKEDIDRGIKLGANDYIIKSDYSMGEILDKIRKLTE"
     gene            3944..>4813
                     /locus_tag="PLF29_02820"
     CDS             3944..>4813
                     /locus_tag="PLF29_02820"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HXK52893.1"
                     /translation="MLYQNTALYNKIKELNVIDSKSLESCLKEAESQNLPLDVILTKK
                     DLIKDNDVGRLISELISFPYVTIKDESIPKDILNIIPEIVAKKQSAVLFDKNKEGLKL
                     ATTNPLNNEFFDMIRKKTGEFLSIYYTTEKNIQEALSLYNEPLQKAYESLLITHIKNS
                     EKDKNTEVPVSKIVDTLVQYAYQNKASDIHIEPKDNASTIRFRIDGVLHEVLSIPLEL
                     HEKITSRIKVMSKLRTDEHMSAQDGKLNVALQMENLDIRVSIVPIVKGEKIVLRLLSS
                     NSRQFSLQDLGMDE"
BASE COUNT         1778 a          869 c          903 g         1263 t
ORIGIN      
        1 actgcctcct tatatttcaa tactttgcat atacaccacg ctagccttcg cgatatattt
       61 gctgagaaat aaggagaatg ttgcggaaaa aaacattaaa aattacaaac atttggtgta
      121 tctcatattc ttcgtttttt cctggcaaac aagcgaattt atggcagtaa acctaagcct
      181 gcagcaagaa caaagcgcga tattatggaa aattacactg tcattatcta taacagctgc
      241 actctcaact ctatatttta tagtcaattt tctacagatt aaaaatagac gcatactcta
      301 catctcaaca ttacttgtct tcatagaaac tataaatgtc ctatttacac aaaatgtgat
      361 atcagcaaca tacaaaacaa gcaacggaca gactcttttt gaaaaaggaa gcatttcggc
      421 cgggattggg cataacatct tgctggtcgc cctgcttctt ctctccttag gctttatagt
      481 caatgcctta aagataaatt cagttaaaag agcctcgatt cgttttctta tgtttggagt
      541 aatttcggtt atcttaccgc agattctttt tggagttatt tttccaatat ttaatctacc
      601 tacagtaaga attggacaaa taagtataat attcgttgtt tttttcttct acaaagccgt
      661 cagtgaaaaa agctcaatca ttctatcaaa gcagtatatt tctattaaaa caaagtttat
      721 ttttgttttc gcgctaatct cgctcggcgc tgtgatcttg accgctatac caacctccac
      781 tttacttaaa aatctaagcg agagaactca ggcaaaaaac atttcaatac aagtcgaaaa
      841 cgcttcaaaa gacatcaata cttttttaga cgagtacgca tcggatgctt tatttctttc
      901 taatctcggc acagtgcgaa gactagagat tgatcaaatt caaaacgaat ttttctctct
      961 tgcagaatca aactcggact atatgcaagt gaggctcata gacattaatg accaagaaat
     1021 tgtgcgggtg gactcggacg aaaaaagtac tcgaatcata gatgaggaca agcttcagaa
     1081 taaagtagat aggtattact tcactgacag catcgttttg aatcaaaaag aggtatatat
     1141 gtcgaaaatt gatttaaaca gggaaggcag tcctccacaa atagaaattc caattaatcc
     1201 tacagtgcga tttgcaaccc ctttttatag ccccgatggt gaaaagctag gcattgtgat
     1261 tctaaatgtc catgctaata agttgctaga gagccttaac cacaacaatg acgtgaggtt
     1321 tacggacgaa tccggttatt ttattgcgca ccctaatagt gagcttgagt ggggaagtcc
     1381 tgcagacttc gataccggct ataatttctc gctggagctt agcagtcaag atatagaagg
     1441 aatgagatca gaacaaacat attatagtaa aggacagcaa ggactggttt atgctaataa
     1501 actcacgcta tataacaata ctttttttgt atatacagca cttgattaca gcgtaatatt
     1561 gcaggacatt aataacctcc aaatctctct gctatcttta ggagcactgt actcgatact
     1621 ttctcttgtt tttgctgaaa ttgcttctgg ttttgttgtt aaaaatttaa ttgaaatcag
     1681 aaaaacagta gaaagaataa ataaaacaaa agaaatagaa tatctcacaa taaattccca
     1741 cgacgaaata ggagaaattt caatattact taacgaatgg gcaagaaaaa taaatcttac
     1801 gaataatgaa atacaaaaaa cagtagataa ccaagttcgt caaataaaaa acaatgagga
     1861 taagctcaaa gaacaacaaa aagcactaat gaatgtactt gaagacgttc aagaggagaa
     1921 acaaaaggcc gaacagcatt cgacagaact tataaagttc aaacttgcgg tcgaaaactc
     1981 ttcagaccaa atagtcatta cggacccgga gggttatata ttgtatgcaa atccaatact
     2041 ggaaaaaata acaggctttt cggtagaaga aagtgttgga aaaaaagcag gctcgcgcga
     2101 actgtgggga ggaataatgg agcctgaatt ctacgaagtt atgtggacta aaataaaaaa
     2161 agaaaaagtg ctattcaaag gacaaataaa aaataggaaa aagtctggcg aagagtacac
     2221 agcctccgtc actatagtac cgatactaaa cgataaggga gaaacaatat tcttccttgg
     2281 ggtcgaaagg gacattacca aagaggcaga ggttgacaag atgaaaaccg aatttatttc
     2341 attagcatct catcaactta gaactcccct ttctgcaatt aagtggtttt tggagatgct
     2401 tatagacggc gatcttggta cattgaatga ggaacagcaa aaaactttac tggacgtaaa
     2461 tacctcaaac gagagaatga tatccttagt aaactcgtta ttaaatgtat caagaataga
     2521 atcgggaaga ataataattg accccgaacc cactgagttg aaagacttaa ttgacggagt
     2581 acttatggag ctaactcctc agataaaaga gaaagaaata tctacaattg taagcatcgg
     2641 cgacaacatg gaaaaaattt caatagatcc aaaactaata agaaatgttt atcttaattt
     2701 gatttcaaac gccgtaaaat acacctcaaa aaacggcgag atacagattt ttgtctcaca
     2761 taacgatacc gaggtaatat ctgcgattag cgacgacggc ataggcatac ctaaagaaca
     2821 gcaagataga attttccaga agttctttag ggcagacaat gtcataaagg ttgaaacaga
     2881 aggcaatgga ttgggcttat acttagttaa agcaattgta gagtcttccg gaggaaaagt
     2941 gtggttttcc agtactgaag gtgaaggaac tactttttgg tttagtttgc ctaagaaagg
     3001 ttccatgaaa aagaaaggtg aagtatcaat agattcttga taatatattt ttatgaacac
     3061 aagctctaaa caaaaaccaa aaaaccttag cacaattcta gttgtagaag acgaagcgtt
     3121 actattgaca gcaatctcag caaaactaaa taaacttggc attgaagccg tcacagtaac
     3181 aagcggagct caggccctag actacctcga aaacctacag caatactccg ccctgccgga
     3241 tgcaatttgg cttgactatc atctcaaaga catggacggt cttcagttta tgagcgctat
     3301 aagaaaaaac cccgagcttt ctaaaatacc ggtaataata gtaagtaaca gcgcgtccaa
     3361 cgacaaagtt gagagtatgc ttgccttagg agcaaaaaaa taccttttaa aagctgagca
     3421 caggctagac gagatagtaa atatttttgg agaactagtt gaaaatgaat aacataacag
     3481 acagcagttt tataaataag ggcagcccct cattaaacca gcgacctgac cagagtgtac
     3541 ctacaaacaa agatactaaa ggcgcctcaa ataataaaca tgtgctgatt gcagaggatg
     3601 ataaattctt aggaaacgcg tatagggtaa agttggagaa aagcggctat caagtaacgc
     3661 tagcaaggga tggggtagaa gcgatggaag aattatcaaa aggttcagat ttttgtctga
     3721 tcattttaga ccttataatg ccaaaaaagg acggcttcgc taccctggaa gaaataaaac
     3781 agcaacctag cctcaaagac attccaattt taatagcttc aaatcttgga caaaaagaag
     3841 atattgatag aggtataaaa cttggtgcta acgactacat tataaaaagc gactactcga
     3901 tgggagaaat attggacaaa attagaaagc taactgaata aaaatgcttt accagaacac
     3961 tgccctctac aacaagatca aagagttaaa tgtgatcgat tccaagtcac tggaaagttg
     4021 cttaaaagag gcggaatctc aaaacttacc tctggatgtt attctaacaa agaaagatct
     4081 cattaaagat aacgatgtgg gaagattgat atcagagttg atttcgtttc catatgtaac
     4141 aataaaggac gagtcaatac caaaagatat tctcaatata atccccgaga tagtagcaaa
     4201 aaaacaaagc gccgtgcttt tcgataaaaa taaagaggga ctaaagcttg cgacaacaaa
     4261 tcctttaaat aatgagttct ttgacatgat taggaaaaaa accggcgaat ttttatctat
     4321 ttactacact acagagaaaa atattcaaga agcactgtcc ttatacaacg agcccctaca
     4381 aaaagcttat gagagcttgc tgataacaca tattaaaaat tcagagaaag acaaaaacac
     4441 agaggttcct gtgtctaaga tagtagatac attggtccag tacgcatatc agaataaagc
     4501 atcagacatt cacatcgaac cgaaagacaa tgcgtctaca ataagattta gaattgatgg
     4561 ggttttgcat gaagttcttt caatacctct ggagctgcat gaaaaaataa catccagaat
     4621 caaggtgatg tcaaaattac gcaccgacga acacatgagc gctcaagacg gcaaactaaa
     4681 cgtagccctg caaatggaga acctagatat tagagtatcc atagtcccaa ttgtaaaagg
     4741 ggagaagata gttcttagat tactctcatc aaactctagg caattctcac ttcaagatct
     4801 gggcatggat gaa
//
LOCUS       DAOSWN010000051         4742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_12180_length_4742_cov_4.066780, whole genome shotgun sequence.
ACCESSION   DAOSWN010000051 DAOSWN010000000
VERSION     DAOSWN010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4742
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12180_length_4742_cov_4.066780"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            299..1477
                     /locus_tag="PLF29_02825"
     CDS             299..1477
                     /locus_tag="PLF29_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52894.1"
                     /translation="MTKGIVGLLPLPIVALHLTYLIAFKKQLYRLRPVIIHFFILIGA
                     AVAVFGPWHFYMFSRLGREFYDVYIQYHVLDRATQAIEDKGQPFHWYLLVMKVSMRIW
                     FIVLFGALSHKILCRLSQNRRVGFIIDALSPKLANLFSIKKFLSIAPSNKDKLLFLIS
                     GFIFFFFSASRSKLVWYIIPIYPFLSIIVGSFIAQVIHSFRIGVVARAVIVYSILTLS
                     LFYFYTQRDLVYTYDFTGKQASLMELKNISYGEKEKLYIDASVELPLALYYVDGPFEI
                     LEYKPLRQKVADTKYDEDVIFLTKESRYRNFNNENPTLTIVERIDEWVLGYKPSQRSL
                     DRKALKDAQRELTELNEDMGRRTAAGEKISLEQSSRRQELTDLVNNLTLTIDKNGDSN
                     "
     gene            complement(1445..2635)
                     /locus_tag="PLF29_02830"
     CDS             complement(1445..2635)
                     /locus_tag="PLF29_02830"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HXK52895.1"
                     /translation="MRKFLYNISTVAFGNFFNAALGFFYIAAVAKALTLEDFGKYSIL
                     SATLVGIAKLTDFGVNNVFVAKSITQTDAIEKLRHQFGGAKITLLVLAIFIGCLFIFS
                     AGYREYSLLILFIIGVVGYSVNFSLFALFQKEKLYIYAVALNTLPALIKGLIAIMLFF
                     GFFNLSLFSAFAVFSLSICTSLILYTKLPEKHTLYSFPSKKTFELLRTAAPAGVSGLI
                     GSSWSAIAAFIAKVFGTFSSAGIYSIADKIANVFTLVSVSISTVLLPNLAQSKLDNKR
                     INKKKLGVLCILTATMGLLTVGATRVLFPIVFGDKFADSINLLDILIIAASFTAIHNF
                     LENYFFVEQATHKLAAISLLKLTVFVAVAFSLMGTYGLTGLAIAQISASTVALITISV
                     FIYR"
     gene            2670..3770
                     /locus_tag="PLF29_02835"
     CDS             2670..3770
                     /locus_tag="PLF29_02835"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HXK52896.1"
                     /translation="MKIGIDCRMWNETGIGRFTRNIVEGLVESSPESEFVLFVLKRDY
                     DQVLAAPNVKKVIVDIRWYTFKEQLVLPMYLYREGLDVFLSTNLNVPVLYFKPTVVTL
                     HDLTILKVKTGRASTHFYLFYLLKRLGARIAILFSIFFAKRILTVSNFVKKELVSDYK
                     INSNKIEVIYNAVSESFFPQEESKIDKVLEKYDIKKPYLFYVGNAHPHKNICALIEGY
                     LIAKKNYPDLSLVLAGKKDFFYERLESNYSEEGIKYTGFVEDTDLPSLYSGSEAFITA
                     STHEGFGLQLLEAFACGTKVICSNTTSLPEVGQDIPFYVDPYNSNDVARGIEGALSAN
                     GRDLRLRGFERVKNFSWKISAEKVLKVLNEVA"
     gene            3757..>4742
                     /locus_tag="PLF29_02840"
     CDS             3757..>4742
                     /locus_tag="PLF29_02840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXK52897.1"
                     /translation="MKSHNSPTPKIALVFDDLVQFGGAERLLLAFFEIWPDAELFTSV
                     ISHRWRKRLKGLGIKYHLSFMQSLPFCCRLNKFYSILGLHCIAFENFHLNDFDLVISI
                     SARYAHGVITKPQTLHISYCNSPGRMFWEPEYYFKNKSWLRKILGLSLSYLRLWDRSV
                     SARADVVIANSRTVRDRLQKYNGIYADKVIYPFVDFTEIRRIISKNEESVVAGKPYFL
                     VVSRLVSWKRVDTVVEAFNSLGINLVVVGEGPDFSRLKSLACQNVTFLGYVPEDRKII
                     LMKNAEAVIIPQIEDFGIVPLESMACGTPVIAYNSGGSTETVVPGISGILYK"
BASE COUNT         1415 a          822 c          989 g         1516 t
ORIGIN      
        1 ttttagtgcg ctggtattac ttacaaatgt atcttttatt tattatgggc gtgcttctat
       61 gcttgatgtt acaacaacat tttttatcac cgcatccctc ctgttttatt atttattttg
      121 cagcagcttt tttgttggtt tgtttgaccg tgttcccatt gcaggtacga gtaaaagtat
      181 ttcttctgaa gccaagattc tcagtttaat tctaattccc ttacagaaac tgtatagagc
      241 cctgacctct acagaagccc tcatgtttta tgcaggtttg tgtgtaggcc tagccgttat
      301 gacaaaaggt attgtcggtt tgttaccctt gccgatagtt gctctgcact taacatattt
      361 gatcgcgttt aaaaaacagc tttatcgttt aaggcctgtt attattcatt ttttcatact
      421 gattggtgcg gctgtggccg tctttggtcc ttggcacttt tatatgtttt ctcgcttggg
      481 gagagaattt tatgatgtat atattcaata tcatgttctt gacagggcta cgcaagctat
      541 agaagacaaa gggcagccgt tccattggta tttgctggtc atgaaagtaa gcatgcggat
      601 ttggtttata gttttattcg gcgcactctc tcataagatt ttatgcagac tgtcccaaaa
      661 cagacgtgtc ggttttataa ttgacgccct ttctccaaag ctcgctaatc tattttctat
      721 aaaaaagttt ctttccattg ctccttcgaa taaagataag ctattgtttc taatttcagg
      781 cttcatcttt ttcttttttt cagcttctcg ttcgaagctt gtttggtata ttattcccat
      841 ctatcccttt ttatccatta tagtcgggag ctttatagca caagtaatcc actcattccg
      901 aatcggtgta gtggcacggg ctgtaatagt atattcaatt ctgacactaa gtttatttta
      961 tttttatact caaagagatt tagtctatac gtatgatttc accggaaaac aggcttcgtt
     1021 gatggagtta aagaatataa gctatggcga aaaagaaaag ctatatattg acgcatcggt
     1081 ggaattacct ttggctttgt actatgtaga cggccctttt gaaattttgg aatacaaacc
     1141 attgcggcaa aaagttgcag atactaagta tgatgaagac gttatatttt taactaaaga
     1201 gagcagatat agaaatttca acaacgagaa tccaaccctc acaattgttg aaaggattga
     1261 tgagtgggtt ttagggtata aaccttcgca acgtagcttg gacagaaaag cgttaaagga
     1321 tgctcaaagg gaacttactg agttaaatga agacatgggc agacgtaccg ctgccggaga
     1381 aaagataagc cttgagcaaa gctcgagacg ccaggagctt actgacctgg taaacaattt
     1441 gactttaacg atagataaaa acggagatag taattaaagc tacggtagat gctgatattt
     1501 gcgcaatagc taagcctgtg agtccgtaag tacccattag agaaaaagcc accgctacaa
     1561 agacagtaag ttttaacagc gatatggcag caagtttgtg cgtggcttgt tccacaaaaa
     1621 aataattttc aagaaaatta tgtatagcag taaaagacgc agcaattatc agaatgtcca
     1681 gaaggtttat tgagtctgca aatttatcgc caaaaactat tgggaataga actcttgtag
     1741 cccctacagt tagcaggccc atggtagctg ttaatataca taagacgcct aacttttttt
     1801 tgtttatacg tttattatcg agcttagatt gagccaggtt tggtagcaga accgtagaga
     1861 tggagacgga aaccagtgtg aaaacgttcg caatcttatc tgctatagag tatatgccgg
     1921 cacttgagaa agtgccaaaa acctttgcta taaaagccgc aattgccgac cacgaagatc
     1981 cgattagacc tgaaacaccc gccggagctg ctgtgcgaag tagctcaaaa gtttttttgg
     2041 aaggaaagct gtaaagtgtg tgtttctcag gcaatttggt gtataggatt aaactggtac
     2101 aaatagataa gctaaatact gcaaaagcgg agaaaaggct taggttgaag aatccgaaaa
     2161 aaagcatgat cgcaatcagc cccttaatta gtgcaggcag tgtatttaac gcgaccgcgt
     2221 aaatatagag tttttctttt tgaaacaaag caaaaagaga aaaatttacg ctatagccaa
     2281 ctactcctat gataaataaa atcagcagag agtattccct gtaccctgca gaaaagataa
     2341 ataggcagcc aataaatatt gctaggacaa gtagtgtgat tttcgcaccg ccgaattgat
     2401 gtcggagttt ttctatagca tcggtttgtg taatactttt tgcaacaaaa acattattaa
     2461 caccaaaatc cgttaatttt gctattccca caagcgtagc agatagtatg ctgtattttc
     2521 cgaagtcctc gagggttagt gcctttgcca ctgcagcaat ataaaagaat cccagcgcag
     2581 cattaaaaaa atttccaaat gctactgtgg atatgttata caaaaacttt ctcatatttg
     2641 aatgttatca ttgtataatt aatttacata tgaaaatagg tattgattgt agaatgtgga
     2701 atgaaaccgg cataggaaga tttactcgaa acatagttga agggcttgtt gaaagttccc
     2761 cagagagtga gtttgtatta tttgtattga aaagagatta cgatcaagtc ttggccgccc
     2821 cgaacgtaaa aaaagtcata gtggatatta ggtggtacac cttcaaggag cagcttgttt
     2881 taccaatgta tctttataga gaaggtcttg atgtattttt aagtacaaat ttaaatgttc
     2941 cggtgttata ttttaagcct acggtagtga cactccatga ccttacaata ctgaaggtaa
     3001 aaacaggcag agcctctacc catttttatt tgttttattt gttaaagcgt cttggagctc
     3061 ggattgcaat tttattttca attttctttg caaagagaat cttgaccgtc tctaattttg
     3121 tgaagaaaga attagttagc gattataaaa ttaactcaaa taagatcgaa gtaatctata
     3181 atgctgtatc tgaaagtttc ttccctcagg aagaaagtaa aattgacaaa gtgttagaaa
     3241 aatatgatat caaaaaaccg tatttatttt atgtcggcaa cgcacaccct cataagaata
     3301 tctgtgctct cattgaaggc tatttaattg cgaagaaaaa ctatcccgac cttagtcttg
     3361 tgcttgcagg gaaaaaagac tttttttatg aacgtctgga gagtaattat tcggaagaag
     3421 gtataaaata caccgggttt gtggaggata cggatttacc ttctttgtat tcaggcagtg
     3481 aggcttttat aacagcttca acgcacgaag gttttggatt acagcttctt gaggcttttg
     3541 cctgtggaac taaggtaatt tgttcaaata ctacatctct gcctgaggtt gggcaggaca
     3601 ttccatttta tgtagaccct tataacagta atgatgtggc gcgaggtata gagggggctc
     3661 tctctgccaa tggacgtgat cttcggcttc gtggtttcga gcgagtcaag aatttttcat
     3721 ggaaaatttc tgctgagaaa gttttaaaag tattgaatga agtcgcataa ttctcctaca
     3781 ccaaagatag cactagtatt tgatgacctt gttcagtttg gcggggctga aagattactt
     3841 ctcgcgttct ttgagatctg gcccgatgcg gaacttttta caagtgttat atcgcacagg
     3901 tggagaaaac gtcttaaggg cctaggcatt aagtatcatt tgtcctttat gcaaagtctg
     3961 cctttttgtt gcaggcttaa taaattttat tcaatattgg gcctccactg tattgcattt
     4021 gaaaactttc atttaaatga tttcgatctg gtaatttcaa tttctgctcg ttatgcgcat
     4081 ggagttatta ctaaaccgca aactttgcat atatcctatt gtaatagccc cggaagaatg
     4141 ttctgggagc ccgagtatta ttttaaaaat aaatcttggc tgagaaaaat tcttggtttg
     4201 agtttaagct atttgcgttt gtgggaccgt agtgtttcag cgagagccga tgtagtcatt
     4261 gctaactccc gaaccgtgcg agacaggtta caaaaatata atggaatata tgcggataaa
     4321 gtaatttacc catttgtaga tttcacagag attagaagaa ttatatctaa aaatgaggaa
     4381 agtgtggttg ccggaaagcc gtattttttg gtcgtttcaa ggctggtttc atggaaaagg
     4441 gtagatactg ttgtcgaagc ttttaattct cttgggatta atcttgtagt agttggtgag
     4501 ggccctgatt tttctcgact taaatctctc gcctgtcaaa acgtaacttt tttaggctat
     4561 gttcctgaag atcgaaaaat tattcttatg aaaaacgccg aagccgtaat aattcctcag
     4621 attgaagatt ttggaatcgt ccctcttgag agcatggctt gcggcacgcc tgtaatagca
     4681 tataattcgg gaggttctac cgaaacagtt gtacccggca tttcggggat tctttacaaa
     4741 ga
//
LOCUS       DAOSWN010000052         4695 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_12366_length_4695_cov_4.471552, whole genome shotgun sequence.
ACCESSION   DAOSWN010000052 DAOSWN010000000
VERSION     DAOSWN010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12366_length_4695_cov_4.471552"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..256
                     /gene="secG"
                     /locus_tag="PLF29_02845"
     CDS             38..256
                     /gene="secG"
                     /locus_tag="PLF29_02845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HXK52898.1"
                     /translation="MLNVLKALQLFLSVFIIFVVVIQTKTGGLSSSISGAVSAYRSKR
                     GLERLILILTIISALFLVVNSLAIVILS"
     gene            260..1585
                     /locus_tag="PLF29_02850"
     CDS             260..1585
                     /locus_tag="PLF29_02850"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HXK52899.1"
                     /translation="MKPSVDIQGGNPLKGSIKISGSHIAASKIIYASLFVSEDVVLEN
                     VPNVDTVKSDLSLIKALGASYEWIGKNSLLINAANLTSFEVPFEIGSQLRTTMLVVGP
                     LLHKFGKACVPLPLNIKAGFRPINRWFDVWDAFGVKIREKDGIVTFDSSEVRAADIKF
                     KGITNSGTDNAILTASFINGESTISNASQSPETDNLIDFVNSIGAKVERVEPDVIKVT
                     GTSTFSSAKHRIIFDKNEAVLFMLLALITNGNLILKGTERGPLLSFLSSLPKIGATFE
                     FANDEIRIWRIGEELQPINITSAPSPAYLTDWISPMCALMTQVEGTSVFHDTVYVDNF
                     GYVTQLNSMGANIELKRPSEVGLECVISNDAYNLKVEGEPFTVAHVTGKTKLKGRSLH
                     IEGYNSGLALVSAALCAEGKSTVIGIEKVEQYTEDFIEKLLSLGASIER"
     gene            1606..2706
                     /locus_tag="PLF29_02855"
     CDS             1606..2706
                     /locus_tag="PLF29_02855"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HXK52900.1"
                     /translation="MLNQILYLIFAMMISFALYPVWISFVYKYQMGEDVREDGPQSHL
                     KKKGTPTMGGLVFLVTVSLVTFIFNKSRTQTLFPLLVAAMAGLFGIVEDLSKLYRTSG
                     LRQLLMIDGAFNYKKGDLASNIAKKTVFKPFIKVWRFFFEFTRIIGSTANIGLQTYQK
                     LLIQGFIGGFVAYWAYVKLGWDYIWFPLIGDVHIGILYPIFIFFLFIAVLNFVAFTDG
                     LDGLAGGLGIIAFAAFWVLASRLGYNSLGTLAATFIGAMLPFLYFNVYPARIFMGNVG
                     SHVLGSVLAVFAVVLHREVALFFILGVYLMDGISSPLQQFSNKLTGKRIFRMAPMHHH
                     FELLGWPETKVVLRFWIVSVMLTFVGILIALL"
     gene            2763..3965
                     /locus_tag="PLF29_02860"
     CDS             2763..3965
                     /locus_tag="PLF29_02860"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HXK52901.1"
                     /translation="MSTLPVNYKSKYRLKVRNPKRFPDKILSFCIVFLLSFGIIAIYD
                     STGILSQQIYKTPYKFVLLQLSWLAVGAVGFIIFYNLELSYIKRVARPLFYVTASLLA
                     LLAIFGVLPCSLNFIFTPCVNGANRWLYFNPHPLPPLPVLGVLGFQPSELAKFSAVLF
                     FASFLSDMKTSIKDETIKFFIFLGSVSLLIVLQPNMSTAALIFAIGLSMYFASGFSIK
                     PFLLALPPFTLLGLLAMLASPHSRARIERFTAAFSGNADANVVGYHVKQILIALGSGG
                     IFGVGFGQSRQKFNYLPEVFADSIFAIIGEELGFVGTVSLVFVFGLLIYKGYSVALKS
                     KDMYARLLAVGITSWIAIQFFVNISAMTQLIPLTGMPLPLISYGGSSLVFMLMGIGVL
                     SNISRMKS"
     gene            3984..>4695
                     /locus_tag="PLF29_02865"
     CDS             3984..>4695
                     /locus_tag="PLF29_02865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXK52902.1"
                     /translation="MKIVLIGGHHSSAIPVIKELQKSDSSLELVWFGHRNSTRSNKRE
                     SLEHKEITALGLKFYSLRAGKFYRTYNPISLLMIPLGFVHALLLLIKERPDAILTFGG
                     YLSVPVVTVGWFLRIPSVLHEQTVVVGYANKFSSFFAKRVLLSWPDSIKFFNKKKVQV
                     VGIPIRSEIFEASSASFICEPSLPVMYITAGKSGSNKINLLINSILEHLLDEVNIIHQ
                     CGDHSQFNDYDKLLNTYNN"
BASE COUNT         1282 a          846 c          975 g         1592 t
ORIGIN      
        1 tatggttagg caaggaaaga tagtattatt ataaaatatg ttaaatgtat taaaagcact
       61 tcaattattt ttatctgttt ttataatatt tgttgtagtt atacaaacaa aaacaggcgg
      121 tctttccagc agcattagtg gtgcagtaag tgcctacagg tcaaaaagag gcttagagag
      181 acttatactc atattaacta taatttctgc actcttttta gtcgttaact ctctagcaat
      241 agtaatttta agttaaaaca tgaaaccctc agttgacatc caaggcggaa atcctctaaa
      301 aggaagtata aagatttccg gttctcatat tgctgcatct aaaatcatat atgccagttt
      361 atttgtttcc gaagatgtgg tgttagaaaa tgttccaaat gttgatacag taaaaagtga
      421 tctaagctta attaaagcct taggtgcaag ctacgagtgg atagggaaaa actctttact
      481 tattaatgcc gcaaacctaa cctcttttga agttcccttt gaaatcggtt ctcaattacg
      541 tacaacaatg ttggtggtgg gtcctctgtt gcacaagttc ggtaaagcct gtgtaccttt
      601 acctttaaac atcaaggccg gctttaggcc tataaacagg tggttcgatg tttgggacgc
      661 ttttggtgta aaaataagag aaaaagatgg cattgtgact tttgatagtt ccgaggttag
      721 ggcagccgat ataaagttta agggtataac taattccgga accgacaatg ctattttaac
      781 tgcgtcattt atcaacggag aatcaacgat ctcgaacgca tcccaatctc cggaaacaga
      841 caatttaata gattttgtga atagcatagg tgcaaaagtt gaaagagtag agccggacgt
      901 tattaaagtc acaggcacaa gcactttcag tagcgccaag cacagaatta tatttgataa
      961 gaatgaggcc gttttattta tgctgctcgc attgattaca aacggaaacc ttattttgaa
     1021 gggtaccgaa agaggtcccc ttttatcttt tttgagttct ctacctaaaa ttggagccac
     1081 gtttgagttc gcaaatgatg aaatcaggat ttggcgaatt ggcgaagagc ttcagccgat
     1141 aaatattacc tcggcccctt ccccggccta cctcactgat tggattagcc ctatgtgcgc
     1201 acttatgacg caggtagagg gcacaagcgt gtttcatgac acagtgtatg ttgacaactt
     1261 cggctacgtt acgcaattaa atagtatggg agcaaatatc gagctcaaaa gaccttcaga
     1321 agtaggtttg gagtgtgtta tcagcaacga tgcttataat ttaaaagtag agggcgagcc
     1381 ttttacagtg gcccacgtta caggaaagac aaaactaaaa ggaagatcat tgcatataga
     1441 aggttataac tcaggacttg ctttggtgtc tgccgcgtta tgcgcggagg ggaaaagtac
     1501 tgttattggc attgaaaagg ttgagcaata tactgaggat tttattgaaa agcttttgtc
     1561 tttaggcgct tctatagaga gataaattag tgctatactc ttaatatgct taatcagatt
     1621 ttatacttga ttttcgcaat gatgatatct tttgctcttt atccggtttg gataagtttt
     1681 gtatacaagt atcaaatggg agaggatgtt cgagaagatg gaccacaatc tcatttaaag
     1741 aaaaaaggca ctcccacaat gggaggtttg gtttttcttg ttactgtttc tttagttact
     1801 tttatattca ataaatctcg tactcaaacc ctttttcctc tattagtggc tgccatggca
     1861 ggactttttg gtattgtaga ggatttatca aaactttaca ggacttcggg cttgaggcag
     1921 cttcttatga ttgatggtgc gtttaattat aaaaaaggcg atctggcctc aaatattgca
     1981 aaaaaaacag tctttaaacc ttttattaag gtttggaggt tcttttttga gttcacaaga
     2041 attattggga gcactgcaaa cattggttta cagacatatc aaaaacttct aattcaggga
     2101 tttattggtg gttttgtagc ctattgggcc tacgtaaaat taggttggga ctatatttgg
     2161 tttccgttga ttggcgacgt acacataggt attctgtatc ctatttttat tttcttttta
     2221 tttattgcgg ttttgaattt tgttgctttt acagacggct tggatggctt agcaggaggc
     2281 ctaggtataa ttgctttcgc agctttttgg gttttagctt ctcgtctcgg ctacaattct
     2341 ttgggaacct tagccgccac ttttattgga gcaatgttgc cctttttgta tttcaatgtc
     2401 taccccgcaa gaattttcat gggaaatgta ggatcgcatg ttctggggtc cgtacttgcc
     2461 gtattcgccg ttgtacttca cagggaagta gcgctgtttt ttattttagg cgtttattta
     2521 atggatggaa tatcaagtcc tcttcagcag ttttcaaaca agctaacagg aaaaaggatt
     2581 ttcagaatgg ctcctatgca tcaccacttt gagcttctag gctggcctga aacaaaggta
     2641 gtattaaggt tctggatagt atctgttatg ttgacatttg tcggcatact cattgcactt
     2701 ctgtaattgc ttcgcaatgt ttatatgagt tcataaaatg acagtaatcg gataccctca
     2761 ttatgagtac attgcccgta aattataaat cgaaatatag acttaaggtc agaaatccta
     2821 agaggtttcc tgataagatt ttatcctttt gtatcgtttt cttgttatca ttcggtatta
     2881 tagcgatcta cgactcaacc ggaattttat ctcaacaaat atataaaact ccctataagt
     2941 ttgttcttct tcagttatcg tggttagcag tcggggcagt aggctttatt attttttata
     3001 atttagagct ttcgtacata aaacgcgttg ctcgtccgct cttttacgta actgcctctt
     3061 tgctggcttt gttagcaatt tttggcgttt tgccttgttc tttgaatttt atatttaccc
     3121 cttgtgttaa tggtgctaat agatggctct atttcaatcc tcatccattg cctcccttac
     3181 ccgttctggg cgttttaggt tttcagccca gcgagctggc gaagttcagt gcggttttat
     3241 tttttgcatc cttcttaagc gacatgaaaa cttcgatcaa agacgaaaca ataaaatttt
     3301 ttattttcct agggagcgta tctcttctta ttgtgctgca gcccaacatg tcgacggccg
     3361 ctctaatttt tgctatcggt ttatccatgt attttgcctc cggttttagc attaagcctt
     3421 ttctgttggc ccttcccccg tttacattat taggcctgtt ggccatgctg gcttctcctc
     3481 atagcagggc tagaattgag cgctttactg ccgcttttag tggaaatgcc gatgcgaatg
     3541 tggttggata tcacgttaag caaattttaa tcgctctagg ttcaggagga atttttggtg
     3601 tagggttcgg acaatcaagg caaaaattta attacttgcc tgaggttttt gcagactcaa
     3661 tttttgcaat cattggcgag gagttagggt ttgttggtac agtatcttta gtttttgttt
     3721 ttggtctgct gatctataaa ggatactctg ttgctttaaa atccaaagat atgtatgcca
     3781 gacttttagc tgtaggcata acgtcttgga ttgcaataca gtttttcgtt aatattagcg
     3841 cgatgaccca attaatacct ctaaccggta tgccgctccc gttaatctct tatggagggt
     3901 cgtcgcttgt ttttatgctc atgggcatag gagtacttag caatatttca cgaatgaagt
     3961 cctaaaatga taaaatcaac catatgaaaa ttgttttgat cggcgggcac cactcttcgg
     4021 caataccggt aattaaggag ctgcaaaaat cggactcatc tcttgaattg gtttggtttg
     4081 gacacaggaa ctcaacacgc tctaataaga gggagtctct ggagcataaa gaaataactg
     4141 cattgggatt gaagttttac tcgcttcggg cgggaaagtt ttaccgaact tataacccta
     4201 tctctttatt aatgattcct cttggttttg ttcatgcgct gctacttcta ataaaagaac
     4261 ggccggatgc aattttgaca tttggcgggt atctatctgt tccggttgta actgtcggat
     4321 ggtttttgag aatcccatct gttttacacg agcagacggt tgtagtgggt tacgcaaaca
     4381 aattctcctc tttttttgca aaaagagttt tactatcttg gccggattcg attaaatttt
     4441 ttaacaaaaa gaaagtacag gttgtaggta tacccattag atcagaaatt tttgaagcca
     4501 gctcagcttc gtttatttgt gagccctctc tgcctgttat gtatatcacg gccggtaaaa
     4561 gcggttctaa taaaattaat ctactaatta actctattct tgagcatctt cttgatgaag
     4621 taaatataat tcatcagtgc ggtgaccact ctcagtttaa tgactacgac aagctcctaa
     4681 atacctataa caatg
//
LOCUS       DAOSWN010000053         4587 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_12768_length_4587_cov_4.863857, whole genome shotgun sequence.
ACCESSION   DAOSWN010000053 DAOSWN010000000
VERSION     DAOSWN010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12768_length_4587_cov_4.863857"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="PLF29_02870"
     CDS             <1..481
                     /locus_tag="PLF29_02870"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52903.1"
                     /translation="VPRGGSLGHTSLPPEKDRTNETQTRLLSMLQMMMGGRAAEEYIF
                     SEISTGAASDIERATELARRMVTEFGMSDLGPVSFDGAGGKFWLAKQMGEMPHYSDST
                     ATKIDDEIRRIIDSSYTKALSILKQNAKGLETVAQALLEKETLDGEEFRALMEKAQG"
     gene            568..1497
                     /locus_tag="PLF29_02875"
     CDS             568..1497
                     /locus_tag="PLF29_02875"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HXK52904.1"
                     /translation="MKKILLIDTFNFIHRAYHALPKTLTSQDGHPINAVYGVTSMLLN
                     IFDAVTPDYVVAAVASRKPTFRLEEFSGYKAHRRPIDDELSEQIPLVFDVLSSFGISQ
                     ISADGYEADDVIGTISTKMSADSNSETIIASNDRDLWQLVRKNVLVVLPATKGVPEWL
                     GPKEVTARLGFEPSLLVDFKGLRGDPSDNIPGVYGVGEKTAKTLIEKYKSVEELYKNI
                     DFVTPESLRNKLLESYEQAIMSKRLARIITDAPIEFNLKETIYQGYDKQTLTQLLQKY
                     NFKSLLKRIGHEGSRDFNDGLGSSSRIMQKSLF"
     gene            1539..2324
                     /gene="nth"
                     /locus_tag="PLF29_02880"
     CDS             1539..2324
                     /gene="nth"
                     /locus_tag="PLF29_02880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HXK52905.1"
                     /translation="MSKLVDVVYPACWALQRKEITANHLLEQVMVDKVFIESANMKNI
                     NKGIKKDLRQHAAEVAEAMKKLYPKPKTELVYANEMQLAIAVMLSAQTTDKKVNEITA
                     ALFKKYLTWDDFAIASLPTLQRDIRGVNFHLGKASRIIKASVIVRDKFGGHLPRTMDE
                     LLLLPGVARKTANVILQELWDITEGIVVDTHVTRISSRLGLSNEKNAVKIEQELMSLF
                     DKKYWRGISGAFVLHGRHVCKARKPECEKCVLKDICPSAFLEN"
     gene            complement(2344..2991)
                     /locus_tag="PLF29_02885"
     CDS             complement(2344..2991)
                     /locus_tag="PLF29_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52906.1"
                     /translation="MGILKDFIVRNKPIFAIGAITALVFLLIIAVGEEQNSSPSQLPI
                     LKKVTESISDKLDSTNGANPQDQNYQQNTSTRESTTYVESKNEAKRLTDLALEQEAAE
                     MERLKKFEETSQMTPAQVDAKYGALIITYTQEGFTPKQTNGYINQLVIWNNDSNKPVI
                     IRQITPSMEAFNEPVIIPVGGRFEMRLTNTGPWAYIEDATNLGGIIMVQNAIFIN"
     gene            complement(3115..3834)
                     /locus_tag="PLF29_02890"
     CDS             complement(3115..3834)
                     /locus_tag="PLF29_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HXK52907.1"
                     /translation="MTDSKNSYQASSAGTPNNYLVMTLMGLLVIASFVIGMLWTKTKT
                     PSAPQGQQAAAVDDKTIRSVLELNDSDHVRGKADSDLVLIEYSDFSCPYCQSYHPNVK
                     AAIEQAGDVAYVYRHFPLPSIHPNAQKQAEASECAAKIGGNEAFWKYADAIFSTKSGT
                     QEQLITLALEQGLDEGSFTSCLTSGETAKKVSSDQENGLKIGIRGTPSSFVINTKTGD
                     FEQIGGAASTETVLQAIEKVR"
     gene            complement(3995..>4587)
                     /locus_tag="PLF29_02895"
     CDS             complement(3995..>4587)
                     /locus_tag="PLF29_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52908.1"
                     /translation="NSIKSYMPNTGTYGQEGRKRWERARGGSNLAGAAQEEMHTAKLA
                     GEEQRKVLAELSSELFSAIASQTGAAKMSESERIQYLKPADEIGTLPITIVLVKHTIG
                     DGREITAVGYVKTPTNTGLGRQAELQPDLSIAIVRGDHETPLPIYGLFPEQVNNTSNA
                     LDREVCARAKFRGSKLVLTEEGVQIAIDVLRGTPQH"
BASE COUNT         1257 a          890 c         1010 g         1430 t
ORIGIN      
        1 agttcctaga ggcggttctc ttgggcatac ttcgttgccg ccggaaaagg atcgcaccaa
       61 cgagacacag actcgtttgc tgtcgatgtt gcagatgatg atgggcgggc gagctgcaga
      121 agaatatatt ttttcagaga tttccacagg agctgcaagc gacatagagc gagcgactga
      181 gctcgcccgc cgcatggtta ccgaatttgg catgagcgat ctgggccctg tttcctttga
      241 tggtgcaggc gggaaatttt ggcttgcgaa acaaatgggc gagatgccac actacagtga
      301 cagtacagct actaaaatcg atgatgagat tagaagaatt attgattctt cctacaccaa
      361 ggctttaagc atactaaaac aaaacgcaaa aggtcttgaa actgtcgccc aagccttgct
      421 tgaaaaggaa actcttgatg gcgaagagtt cagggcttta atggagaaag cacagggata
      481 ggaatcttac acactgtata aggttgcata aggaaaagat tttttattta tcttttttta
      541 tgttctgttt taaggcttgg tataaatatg aaaaagatat tgttgattga cacgtttaat
      601 tttattcata gggcatatca cgcacttcca aaaaccctaa catctcaaga cgggcaccca
      661 ataaacgcag tttatggcgt tacttcgatg ttacttaaca tttttgatgc cgtaactcct
      721 gattatgttg ttgctgcggt tgctagccgc aagccgacat ttagactaga ggaattcagt
      781 ggctacaaag cgcatagaag gcctatagat gacgagctgt ctgaacaaat acctttggtt
      841 tttgatgtac tatcaagctt cggtatttcc caaatatctg ccgatggcta tgaggcagat
      901 gatgttatag gcactatttc tacgaaaatg tcggcggact ctaattcgga aactataatc
      961 gcttcaaatg atagggattt atggcagctg gttaggaaaa atgtgttagt agttcttccc
     1021 gccactaagg gggttccgga atggttaggg cctaaagagg tgaccgcgcg gttaggtttt
     1081 gagccaagcc tcttagtcga ttttaagggt ttaagaggtg acccaagcga caatatccca
     1141 ggagtttatg gggttggaga aaaaaccgca aaaactctta ttgagaaata caaatctgta
     1201 gaggagctgt acaaaaacat tgattttgta actcctgaat cactaagaaa taagctactg
     1261 gagtcttacg aacaagcgat tatgagtaaa agattagctc gtattattac ggatgctcct
     1321 atagaattta atttgaaaga gacaatttac cagggatatg acaagcaaac tttaacgcaa
     1381 cttttacaaa aatataattt caaaagttta ctaaagagaa tcgggcacga aggaagtcga
     1441 gattttaatg atgggttggg gagttcttct agaattatgc agaaaagctt attttagtcg
     1501 ggaattcgaa aatttacaat ttcgttactt gaaactactt gtctaagcta gtagatgtag
     1561 tttacccagc atgttgggct ttgcaacgta aagaaattac agcaaatcat ttgcttgagc
     1621 aggtgatggt tgataaagtt tttatagaaa gtgcgaatat gaaaaatatt aacaaaggaa
     1681 taaagaagga cctacgtcag cacgcggccg aagttgcgga ggcgatgaaa aagctatacc
     1741 ccaagccaaa aaccgagcta gtgtatgcaa atgagatgca gcttgcgatt gctgttatgc
     1801 tgtcggccca aacaactgac aaaaaagtaa acgagatcac cgcagcctta tttaaaaaat
     1861 atctcacttg ggatgatttt gctatagcca gtttgccgac attacagaga gatatacgag
     1921 gagtaaattt tcatttggga aaggccagcc gtattataaa ggcttccgtt attgtacgag
     1981 acaagtttgg gggccattta ccgcggacta tggacgagtt gttacttctt cccggtgtag
     2041 caagaaaaac cgcaaacgtg atcttacagg agctttggga tattactgag ggaattgttg
     2101 tagatactca tgtgacaaga atatcttcga gattggggct ttccaatgaa aaaaacgcag
     2161 ttaaaattga gcaggaactt atgtctttat tcgataaaaa atattggcga ggtatatctg
     2221 gagcgtttgt attacacgga aggcatgtct gtaaggcgag aaagcccgag tgcgaaaaat
     2281 gcgtattaaa agatatttgc ccgtcagctt ttttggaaaa ctaatttcgc cactgaaccg
     2341 tacctaatta ataaaaatgg cgttttgtac cataataatg cctcctaaat tggtggcatc
     2401 ttctatatat gcccaagggc ctgtgtttgt tagacgcatt tcaaaccttc ctccgaccgg
     2461 tataattacc ggttcattaa aggcctccat agagggggtt atctgccgta taataaccgg
     2521 tttgttgcta tcgttgttcc aaataactag ctggtttatg tatccgttgg tttgttttgg
     2581 tgtaaagccc tcttgcgtgt atgttattat taatgccccg tattttgcat ccacttgtgc
     2641 cggggtcatt tgagatgttt cctcaaattt tttaagtctt tccatttcgg ccgcctcttg
     2701 ctccagggct aagtctgtta gccttttagc ttcgttttta gactctacgt acgtggttga
     2761 ttctcttgtg gaggtgtttt gctggtagtt ttggtcttgc ggatttgccc cgtttgtact
     2821 gtcaagcttg tcggatattg attccgtaac tttttttaat attggaagtt gtgaagggga
     2881 gctgttttgt tcttctccaa cggcgataat taataagaaa acaagtgctg taattgcgcc
     2941 tattgcaaaa atcggcttgt ttctaactat gaagtctttt aatataccca tcttaaacat
     3001 tatacaggtt cgctgaggct taaaaaagta gatttggttt tgtattttgt tatggaaaaa
     3061 gtgaggggcg ggcttttaaa gcccgcccct caataaaact caactaaaat gaaattatcg
     3121 aaccttctca atagcttgca gaacagtttc ggtcgaagct gcgccaccta tttgctcgaa
     3181 gtctcccgtt tttgtattaa ttacaaaaga agaaggagtt cccctgatac cgatttttag
     3241 accgttttct tgatcgctgc taactttttt tgcagtttcc cctgaagtta ggcaggatgt
     3301 gaaagatcct tcatctaagc cttgttctaa agctaaagtg atgagctgct cttgagttcc
     3361 tgattttgta ctaaatatag cgtcggcata tttccagaac gcttcgttcc ccccaatttt
     3421 ggctgcgcat tcagaagctt cggcttgttt ttgcgcatta ggatggattg aaggtaaagg
     3481 gaagtgcctg tatacataag ccacgtcccc tgcctgctct attgcagcct ttacgtttgg
     3541 atggtatgac tggcagtagg gacaagagaa atcggaatac tctattaaaa ctaaatcact
     3601 atctgctttt ccccttacgt gatcagaatc atttaattcc aaaacacttc gaatcgtttt
     3661 gtcgtcaaca gcggccgctt gctggccttg tggtgcgcta ggggtttttg tttttgtcca
     3721 aagcatccct attacaaaag aggctataac aagaaggccc attaatgtca ttactaaata
     3781 attgtttggg gtaccggccg aacttgcctg gtacgaattt ttgctgtctg tcataagaaa
     3841 ctctcctaaa cttttaacta actttttctg tttagtaata atagcgacat tcgatcttta
     3901 ttacaagggc tatttatgtt gcagaagagg tttatcttaa tgagcgtagc tcttgcttct
     3961 ttgtatttaa tactgtggta agtctttatg cttttcaatg ttgaggcgta ccccgtaaaa
     4021 catctatagc aatttgtacc ccctcttccg tcagtactaa tttgcttcct ctaaactttg
     4081 ctctggcaca aacctcccta tctagggcat tagaagtatt attaacttgc tcgggaaata
     4141 gcccataaat tggtagtggc gtttcgtggt cgccacggac tattgcgata cttagatctg
     4201 gttgaagttc ggcttgtctt cctaaacctg tgtttgtagg agttttaaca tatcctacgg
     4261 ccgtgatttc cctcccatca cctattgtat gtttgaccaa aactattgtt atgggcaatg
     4321 tgccgatctc gtctgcaggt tttaagtatt gtatcctttc tgactcagac attttagcgg
     4381 ctcctgtttg tgaagctata gcagagaaga gctctgacga gagctccgct aacacctttc
     4441 gttgttcttc tcctgcaagt tttgcagtat gcatctcttc ttgtgcagca cctgcaaggt
     4501 tgctgcctcc tctagctctc tcccaccttt tgcggccctc ctggccatat gttcccgtat
     4561 taggcatata actctttata ctatttt
//
LOCUS       DAOSWN010000054         4574 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_12823_length_4574_cov_4.630671, whole genome shotgun sequence.
ACCESSION   DAOSWN010000054 DAOSWN010000000
VERSION     DAOSWN010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12823_length_4574_cov_4.630671"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="PLF29_02900"
     CDS             <1..424
                     /locus_tag="PLF29_02900"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HXK52909.1"
                     /translation="MLWMEHLVDMDNLRDGIGLRGYAQKDPIVEYKREGHTRFDMLVD
                     SIYSGISERLLRLKEIKRAPEQVRSVENRLESKLKSELQSAVYEQGELNSGVEDEAKL
                     KNKSTKAPTTKSVDKIGRNDPCPCGSGKKYKKCHGKDA"
     gene            580..1212
                     /locus_tag="PLF29_02905"
     CDS             580..1212
                     /locus_tag="PLF29_02905"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HXK52910.1"
                     /translation="MYKNIIFDFDGTIADSFNFLLKVVNSNPSKYGIDGIDANEIPKL
                     RSLSMRYIFREFNINVFKIPRFVKMLREEMRNNLSDLALIEGMAEIISGLQSHGYKIY
                     ILTSNDRRIVTAYLKMHKISGILDIYSGRKLFKKHRLIKKMLRKKHLRKKETLYVGDE
                     IRDIVACEKAGIDIASVTWGFNDSATLAKKSPKFLVDTPEQLKNVILEGR"
     gene            1217..1819
                     /gene="lepB"
                     /locus_tag="PLF29_02910"
     CDS             1217..1819
                     /gene="lepB"
                     /locus_tag="PLF29_02910"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HXK52911.1"
                     /translation="MKKISRPSQQVSDSNEELKKTPFKALLAEIIETVSMSFIVLLLI
                     FTLIAVPEKVDGASMEPTLYDKERVLVEKVSKHFKPYRRGDIVVLHPPGHEQKEYVKR
                     IVALPGEDIKIFNCKVYVSNSDGKFELEEKYLFTDQCTTSGTRFRDGRSLHLEENEFF
                     VLGDNRDVSQDSRIFGIVQSEAIVGKVVFRFWPISDIMFF"
     gene            1912..>4574
                     /locus_tag="PLF29_02915"
     CDS             1912..>4574
                     /locus_tag="PLF29_02915"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HXK52912.1"
                     /translation="MKRHDFSKRKNNQIVPNLIEIQINSYNDLLKNGIAEVLDEIGPV
                     EDFSGRGWVLELSDPSFDKPNISMDEALYKGRTYDAPWYLNAEMRDPINNKKKKQKIY
                     MGDIPLMTPTGTFIINGVERIVVSQLKRSEGVLFTKEIHPSTGAILGGCKILPKNGAW
                     LEVDTTKTGLMSVKIDRRRKIPVSVLLRIFGLETNDDIRAAFEDVDSNPEISFIESTL
                     SKDPSSSYNEAILEIYKKMRPGEPLVLENARSLVQGTFFNRRRYSLGKVGRFKMNQVL
                     GLNFPNEPEHYLLHKDDLVAALKKVININNGIESESDLDHLSNRRVRSVGELLQDEIR
                     IGFLRMERNIKERMSLQPREEMPEPSLLISSRTVASAIHSFFATGQLSQHHDQQNPVT
                     ALDHLRRLSVTGPGGLTKERASMSVRDVHYSSFGKICPVRTPEGPNIGLINYIASYAR
                     VNEYGFLETPYVKLEEKNGKVRITDEVVYLAAYDEELYYITDSTVSIDEKGFITQEQI
                     PLRKGNDFTLGSTAKAQYMELVPGQAVGIMASQIPFLANDDISRALMGTQQMSQAVPL
                     VKVESPIIATGLESDIARNSGIVIVAEDKGEVLYADSTKVKVKYEKAGTAEYLAKKFV
                     KSNSDTSLNQRVKVSTGQKIKKGDIILEGPASQDGEIALGANLNACYMIFEGYEFEDG
                     IILSDRLVTEDVLTSVHIENYKIAVLETKLGPEELTFDIPNISEEALRNLNEDGIISI
                     GAKVKAGDILVGKVAPKGEVELSAEERLLRAIFGEKAKEVRDTSLILPHGKKGVVIGV
                     KRVSKKENSNLPTGVIEEITVYVATQKKIQVGDKLSSRHGNKGVISAILPASDMPHLE
                     DGTIIDVIFSSEAIIKRMNMGQLLETPLSMAG"
BASE COUNT         1538 a          751 c         1021 g         1264 t
ORIGIN      
        1 tatgctatgg atggagcatt tggtagatat ggacaactta cgagatggaa ttgggctcag
       61 aggttacgcg caaaaagatc ctattgttga atataaacga gaaggtcaca ccagatttga
      121 tatgctggtt gatagtatat actcgggtat tagcgagaga ctgctgaggt taaaagaaat
      181 aaaaagagct cctgagcaag ttcgtagcgt agaaaataga ttagaaagca aactaaagag
      241 tgagttgcag tcggccgttt atgagcaggg agagctaaat agcggtgtcg aagacgaagc
      301 aaagttaaaa aataaaagta caaaagcccc aactactaaa tctgtagata agattggtag
      361 aaatgatcct tgtccttgcg ggagcggtaa aaaatacaag aaatgccacg gcaaagacgc
      421 ttaattttat aattttgtta cttatagctg cttgcgtaag ctagtagagg tagcttaccc
      481 agcatgttgg gctttgcaac aaaaagaaat tacagcaaaa cacctgcgta agctggcggt
      541 gattggtagt tttgatgata ttattggaga atttttaaag tgtataaaaa cataatcttt
      601 gattttgacg gtactatagc cgattcattt aactttttat taaaggtagt aaattctaat
      661 ccctcaaaat acggtataga cggtattgat gctaacgaaa ttcctaagtt gaggagtttg
      721 tctatgcgat acatcttccg cgaatttaat ataaatgttt ttaaaatacc tcgttttgta
      781 aaaatgcttc gcgaagagat gcgaaataat ctttccgact tagccctcat tgaaggaatg
      841 gcagagataa tttcagggct gcagtcgcat ggctataaaa tttacattct tacaagcaat
      901 gacaggagaa tagttacggc atatcttaaa atgcacaaaa tatcaggcat tttagacatt
      961 tattcagggc gtaaattgtt taaaaagcat agattaataa aaaaaatgct tcgaaaaaag
     1021 cacttacgaa aaaaggaaac gctctatgtt ggcgatgaga ttagggatat cgtagcttgc
     1081 gaaaaagcag gaattgatat tgcctcggtg acttggggct ttaatgactc agcaacttta
     1141 gcaaaaaaat cacccaagtt tcttgttgat actccggaac aacttaagaa cgtaattttg
     1201 gaaggacggt aattagatga agaaaatcag caggccttca cagcaagtga gcgattcgaa
     1261 tgaggagctt aaaaaaacac ccttcaaagc cttgttggcg gaaataattg aaacagtttc
     1321 catgtctttt attgttctgc tgttaatttt tactcttatt gctgtgccag aaaaagttga
     1381 cggggcaagc atggagccta ctttatacga caaagagagg gttttagtcg aaaaagtttc
     1441 aaagcacttt aaaccttata ggcgcggaga catagttgtg ttgcatcccc cggggcatga
     1501 gcagaaagaa tatgtaaaaa gaatcgtagc tttacctgga gaagatataa aaattttcaa
     1561 ttgtaaggtc tatgtatcta actccgatgg caagtttgag ttagaggaaa agtatttatt
     1621 tacggatcag tgtacaacct caggtacaag atttagagat ggaaggtcgt tgcatcttga
     1681 ggaaaatgaa ttttttgtat tgggggataa tcgagatgtt tcccaagatt caaggatttt
     1741 tggtatagtg caatcagaag ccattgttgg aaaagttgtg tttcgttttt ggccgattag
     1801 tgatatcatg tttttttagt gcgaaactat tgacaggatg ctgttaaaat ggtaactttc
     1861 gctttgtcta ttggcttctt agtttatcaa aattttaagt agaatttttt aatgaaaaga
     1921 cacgattttt caaaaagaaa aaataaccaa atcgtaccaa atttaataga aattcaaatc
     1981 aactcctata acgacctctt gaaaaacggc atagccgagg ttttagatga gattggtccc
     2041 gttgaggact tctcaggtcg cggatgggtt ttagaactct cagatccgtc ttttgacaag
     2101 ccgaacatat caatggacga agctttatac aaaggtcgta cctacgacgc tccttggtat
     2161 ttaaatgctg aaatgcgaga cccgataaac aataagaaaa agaagcaaaa aatatatatg
     2221 ggagatatcc cgttaatgac acctaccggg acctttatta ttaatggtgt tgaaagaata
     2281 gttgttagtc agttaaaaag gtcagaaggt gtgctcttca caaaagagat tcaccccagc
     2341 acaggggcca ttttaggcgg atgtaagatt cttccgaaaa atggggcttg gttagaggtt
     2401 gatacaacaa agaccggttt gatgagcgta aaaatagaca gacgccgaaa aattcctgtt
     2461 tctgtattgc ttagaatttt tggactggag accaatgacg atataagagc tgcctttgaa
     2521 gatgttgatt caaatcccga aattagtttt attgaaagca cactttctaa ggatccgagc
     2581 tcttcctata acgaagcaat tcttgaaata tacaaaaaaa tgagaccggg cgagccttta
     2641 gtgcttgaga atgcccgttc tttagtgcaa ggaaccttct ttaatagaag aagatatagc
     2701 ctaggaaaag tcggtagatt taaaatgaat caggtgttag gtttaaattt ccctaatgag
     2761 ccggaacatt atttacttca caaagatgat cttgtagctg ctttaaaaaa ggtaataaat
     2821 ataaacaacg gtattgaatc ggagagtgac ctggatcact tgtcaaacag aagagtgcgc
     2881 tctgtggggg aattacttca agatgaaatt agaatcggtt tccttcgaat ggagaggaac
     2941 ataaaagaaa gaatgagttt gcagcctcga gaagaaatgc ctgaaccatc tttactcatt
     3001 tcttccagaa cagtagcctc agcgattcac agcttttttg ctaccggtca gctttcacag
     3061 catcatgacc aacaaaatcc ggttacggca ctggatcatt tgcgcagact ttcggttacc
     3121 ggtccgggag gacttacaaa agaaagagca agcatgtctg tacgagacgt tcattattct
     3181 tcattcggca agatctgtcc tgttagaact cccgaaggcc ccaacatcgg tcttattaac
     3241 tacatagctt cgtacgctag ggtaaatgag tatggttttt tagagacacc atatgtaaag
     3301 ctggaagaaa agaacggcaa agtaagaata accgatgagg tggtttactt ggcggcctac
     3361 gacgaagagc tctattacat aacagatagc acagttagta tagatgaaaa gggttttata
     3421 acacaggagc aaattccttt gcgaaaagga aatgacttta ctttaggatc tactgcaaaa
     3481 gctcagtata tggagcttgt tcccggacag gcggttggaa ttatggcaag ccagattcct
     3541 tttttggcaa atgatgatat ttccagagct ttaatgggta ctcagcaaat gtctcaggca
     3601 gtacctttag tgaaagtaga atccccgatt atagctacag gtttggagag cgatattgcc
     3661 cgtaactcgg gaatagtcat agttgcagaa gataaaggag aggttttata cgcagactcc
     3721 acaaaagtaa aagtaaaata tgaaaaagca ggtacggccg aatatttagc taaaaagttt
     3781 gttaaatcaa actccgacac atccttaaac cagagagtaa aggtatctac aggccagaaa
     3841 attaaaaaag gcgacattat attagaaggt ccggcttcgc aggatggtga gattgcttta
     3901 ggagcaaatt taaatgcttg ctatatgatt tttgagggtt atgagtttga agacggaatt
     3961 attttgtcag ataggcttgt tactgaagat gttttaacct cggtccatat agaaaattat
     4021 aagattgcag ttctcgaaac aaagcttgga cctgaagagc taaccttcga tatacctaat
     4081 atttccgaag aagctctaag gaatctaaat gaagacggaa taatatccat aggcgcaaaa
     4141 gtgaaggcag gagatatttt ggtaggtaaa gttgctccaa aaggtgaagt tgagctttct
     4201 gcagaggaaa ggcttctacg agctatattc ggagagaaag caaaagaagt tagagatacc
     4261 tcacttatac ttcctcacgg taaaaaaggt gtggtgatag gggttaaaag agtgagcaag
     4321 aaggaaaact ccaatcttcc gaccggagtt attgaagaaa ttacagtcta tgtcgcaact
     4381 caaaagaaaa tacaagtcgg agacaagctc tcaagtcgac acggaaataa aggagtcatt
     4441 tcagctatct tgcctgcatc ggacatgcct catctagaag acggaaccat aattgacgtt
     4501 attttttctt cggaggccat cattaaaaga atgaatatgg gacagctctt agagacccca
     4561 ttaagcatgg cagg
//
LOCUS       DAOSWN010000055         4456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_13288_length_4456_cov_4.643036, whole genome shotgun sequence.
ACCESSION   DAOSWN010000055 DAOSWN010000000
VERSION     DAOSWN010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13288_length_4456_cov_4.643036"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..1653
                     /gene="proS"
                     /locus_tag="PLF29_02920"
     CDS             184..1653
                     /gene="proS"
                     /locus_tag="PLF29_02920"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HXK52913.1"
                     /translation="MRKNISTRGQDYNQWYQDVIALADLAENSFVRGCMVMKPNGYAI
                     WENIQHELDRRIKETGHKNVYFPLFVPLSSLAKEAEHVAGFAKESAVVTHHRLMSTPQ
                     GIEVDPGSKLDEEVIIRPTSEAIMYQTFSKWIQSWRDLPLLVNQWANVVRWELRTKLF
                     LRTTEFLWQEGHTVHATQAEAQEETLKMLEVYRSFAEEFLAMSPVAGQKSEAEKFPGA
                     LKTYTIEALMQDGKALQAGTSHNLGDNFSKAFDIKYTDENGTLTYAWQTSWGMSTRII
                     GGLIMTHSDDRGLVLPPKVAPVQVVIIPIWKDLEDMKGILAFAEEVKQKLEKVGIRAE
                     LDSRDYQTPGYKFNEWEKRGTPIRIEIGRIELENKELKAVNRVDFEKKTLSAGDCSND
                     ILEMLKNIQQQLLANAKSNREKNTNSVDSYEEFKTILETKKGFIKAFWCQDPKCEEII
                     KQETKATTRCLPLETVEESGKCVYCGKSANHRWLFAQSY"
     gene            complement(1661..3067)
                     /locus_tag="PLF29_02925"
     CDS             complement(1661..3067)
                     /locus_tag="PLF29_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52914.1"
                     /translation="MFKNAQAGGHRGENGGSPDQEKHPNISKRAFLKLLLAASTAAVG
                     KAGYEKLLGRAGAAHLRALAGAVAEVAEDPKEPGRAENYADLYEKYGGKEKLGVSEEM
                     WLEGGVAARLDAVSGLYFDSTLEQGIKIASELSVKYGLRPEYVMPVFYFFCDTKIRSL
                     SKFLSDKAVNLVQTLASNTVDIGDDIICGIDSILRAQRAELKSAHLECDPVRQAAGDI
                     AVAAAGEYTGARAMIQIRTVGFLDMHPSEYALAVKRLIKEGFVRKEDCEDLFGFPYDS
                     IEAVLDLQTKVKESRDDRGSNLTPSDNDYIKAANTVEISVTRNERLLPQGLELAFAYF
                     SMQLSLLRENFSELFVYADAGNVEAQMIVSYLMMQTVDIYATSETEVFDSNHPHYNPS
                     LDEGFTAKYMAEAIAKDFEQLPAYQRLMRIWRRQRGSTFPIINSARGVIEAHLKGPPE
                     PINSLVPSFIKNLLDHSY"
     gene            complement(3085..>4456)
                     /locus_tag="PLF29_02930"
     CDS             complement(3085..>4456)
                     /locus_tag="PLF29_02930"
                     /inference="COORDINATES: protein motif:HMM:NF037020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HXK52915.1"
                     /translation="SPTYFVILAVVLPQSFWIGLLNLIFYAAGLVLVFSFVGLLGQRY
                     LRQIKWISNPKGYFKKVLGATFIILGIFIIAGIDKKVETYILDLGFLDITNFETMLLS
                     KLERESPNKDIFTQEDSKMKNQEIMLNVKYPYKAPSFVGLTSWFNSEPLELEDLKGKV
                     VIVDFWTYSCINCIRTLPYLEKWHEKYSGQGLVIIGVHSPEFVFEKVPDNVEGAVKKY
                     GLQYPIALDSDFKTWRAYDNHYWPAKYFIDKDGKVRHTHFGEGAYEESELIIRQLLGE
                     LNEGSVLDDELVSENKVSGSVSSKQSPETYLNYERGANLSPVNANEFAADKEVFYSIQ
                     TKLSRNEWSLGGTWLMGADHSEARAEGAKLKYNFSAKEVYLVMGPSSIKSADAPKSYK
                     VKVFLNGEAINVEEAGSSVNSDGIVIVDRHDLYKLVNLPEFASDQILELQFDSGVEVN
                     AFTFGS"
BASE COUNT         1406 a          980 c          835 g         1235 t
ORIGIN      
        1 agaattgtac tcgctagcta gtgaggccgc aatcgatcta accattattt gcctgtaaaa
       61 tcgggttaca ctctggtagg gcactgtgta acacagagaa aatcaacata tacccaatat
      121 atcaactttt tttattttgt gaaatttcct tacaaactga atttactgat ataataatgt
      181 gatatgcgaa aaaatatctc tactcgagga caagattaca atcaatggta tcaggacgta
      241 attgccctag cagatttggc ggaaaattct tttgtcagag gctgcatggt tatgaaaccg
      301 aacggctatg ctatatggga aaacattcaa cacgaattgg atagaaggat caaagaaaca
      361 ggacataaaa atgtttattt tcctcttttt gttccactat caagtcttgc taaagaagca
      421 gagcatgtag caggattcgc gaaagaatcg gctgtagtaa ctcaccatag attaatgtca
      481 acacctcaag gaatcgaggt tgaccccgga tcaaaactcg acgaggaagt cattattaga
      541 ccgacctccg aagcaatcat gtatcaaaca ttttcgaaat ggattcaatc ttggcgagat
      601 ttacctttat tggttaatca atgggcaaac gtagtaaggt gggaacttcg aactaagtta
      661 tttttacgaa ctacagaatt cttgtggcaa gaaggccaca cagtgcacgc aacacaagca
      721 gaagctcaag aagaaactct gaagatgctt gaggtgtata ggagctttgc agaggaattt
      781 ctcgcaatgt ctccggtagc cggacaaaaa tctgaggcag aaaaatttcc tggagcatta
      841 aaaacttaca cgattgaagc tcttatgcag gacgggaaag ctcttcaagc aggaacctct
      901 cacaatcttg gcgacaattt ctccaaagct tttgatatta aatacactga tgagaacggt
      961 actcttacat atgcatggca aacaagttgg ggaatgagta caagaattat tggcggactg
     1021 ataatgaccc acagcgacga tagggggcta gtgctacctc ccaaagttgc acccgttcag
     1081 gttgttatta tacctatttg gaaagactta gaggatatga aaggaatctt agcttttgca
     1141 gaagaggtta aacaaaaact agaaaaggta ggtattagag ctgagctgga ttctagagat
     1201 tatcaaactc caggatacaa gtttaatgaa tgggaaaaga ggggtactcc tataagaata
     1261 gagataggga ggatcgagct cgagaacaaa gagttaaaag ctgtaaacag agtagacttc
     1321 gaaaagaaaa cattatcggc aggggactgc tcgaatgata tattagaaat gctcaagaat
     1381 atacagcaac aacttcttgc taacgcaaaa agcaacagag agaaaaacac aaatagtgta
     1441 gacagctacg aagaatttaa aacaatcttg gaaacaaaga agggtttcat aaaagctttt
     1501 tggtgccaag acccaaaatg tgaagaaata attaagcagg aaaccaaagc gaccacaaga
     1561 tgcttacctt tagagacagt agaagaaagc ggtaagtgtg tttactgtgg aaaaagcgca
     1621 aaccacaggt ggctatttgc acaatcatac tagctgggcc ttaatacgaa tgatcaagaa
     1681 gattttttat aaaggagggt acaagagaat ttataggctc aggggggccc ttcaaatgag
     1741 cttcgattac gccgcgtgct gaatttataa taggaaaagt agagccgcgc tgacgtctcc
     1801 aaatcctcat aagcctttga taggcgggca gttgttcgaa gtctttagct atggcttctg
     1861 ccatatactt cgcagtaaaa ccttcgtcca aagaggggtt atagtgcggg tgattgctgt
     1921 caaaaacttc ggtctccgaa gtagcgtaaa tatctacagt ctgcatcatc aaatatgaaa
     1981 ctatcatctg cgcttcaacg tttcctgcat cggcataaac aaaaagttcc gaaaaatttt
     2041 ctctaagaag agataattgc atagaaaaat atgcaaatgc aagctcaagc ccttgtggca
     2101 acaatctttc atttctagta actgaaatct ctacagtgtt tgcagccttt atgtaatcat
     2161 tatcagatgg ggttaagtta ctgcctctgt catctctgct ttctttcact ttcgtctgca
     2221 aatccaaaac agcttctata gaatcatagg ggaaaccaaa caaatcttca caatcttcct
     2281 tgcgcacaaa tccttctttt attagacgct ttactgctaa ggcgtactca gaaggatgca
     2341 tatcaagaaa acctactgtt cgaatttgaa tcatggccct tgccccagta tattcaccag
     2401 cagcggccac agcaatatct cctgccgcct gccgaaccgg atcacattcc aaatgtgcgc
     2461 tcttaagctc cgctctttga gctcttaata tactatctat tccgcatata atgtcgtctc
     2521 ctatatctac agtgttcgat gctaaagttt gaacaagatt tacagcttta tcagataaaa
     2581 atttagacag tgatctaatc ttcgtatcac aaaagaaata aaagaccggc atgacgtact
     2641 caggacgcaa tccatattta acacttagct ccgaggctat tttaataccc tgctctagag
     2701 tactgtcaaa atacaagcca gatactgcat ctagtcttgc ggcaacgcca ccctctaacc
     2761 acatttcctc tgaaactccc agcttctctt tccccccata tttttcgtac aggtcggcat
     2821 aattttcagc acggcccggt tcctttggat cctccgcaac ctctgcaaca gcccccgcaa
     2881 gtgcccgtaa gtgagcagcc cctgctcgcc ctagtagctt ttcatagccg gccttaccga
     2941 ccgccgccgt gcttgcggca agaaggagtt ttaagaaagc acgtttgcta atgttagggt
     3001 gcttttcctg gtctggcgac cccccgtttt ccccacggtg cccaccggcc tgtgcgtttt
     3061 taaacatttg tctggggtct taaactaact accgaaagta aatgcattta cctccacccc
     3121 cgaatcaaat tgcaactcta atatttgatc agatgcaaat tcaggcaaat tcacaagctt
     3181 atatagatca tgccgatcta ctatcacaat gccatcggag tttacggagc ttcccgcctc
     3241 ttcaacattt attgcctctc cgtttaagaa aaccttaact ttatatgatt tgggcgcgtc
     3301 cgcagactta atagaactag gccccatcac taaatacact tctttagctg agaaattgta
     3361 tttaagctta gcgccttcag ccctagcctc agaatgatcc gcccccataa gccatgtccc
     3421 acctaaagac cactcgtttc tactcaattt tgtctgaatg gaataaaaaa cctctttatc
     3481 agcagcaaat tcgttagcat tcaccggcga taaatttgcg cctctttcat aattcaaata
     3541 tgtctcaggg ctttgtttag aagaaacact accagaaact ttattctcag ataccaattc
     3601 atcatcaagc acgctgcctt cattcagttc tccgagtaac tgtcttatta taagctcgct
     3661 ttcctcatac gcgccttctc caaaatgggt gtgcctaact tttccatcct tatcaataaa
     3721 atatttcgca ggccaatagt ggttatcata tgcccgccat gttttgaagt cgctgtctaa
     3781 agcaattggg tactgcagtc catatttttt aaccgcgcct tcaacattat ccggtacttt
     3841 ttcaaaaaca aattcagggg agtgtacccc tattataacc agcccttgtc cagaatattt
     3901 ttcgtgccat ttctcaagat agggaagtgt tctaatacaa ttaatacagc tatatgtcca
     3961 aaaatccact atcacaactt ttccttttaa atcttctaac tccaacggtt cggaattaaa
     4021 ccaagaagtt aagccgacaa aagaaggtgc cttatagggg tatttaacgt ttagcatgat
     4081 ctcttgattt ttcattttac tatcctcctg tgtaaaaatg tctttatttg gagactctct
     4141 ctctagttta gataatagca ttgtctcaaa attagttata tccaaaaaac ctaaatctag
     4201 tatgtaagtt tcaactttct tgtcgatccc tgctattata aaaattccca aaataataaa
     4261 agtcgcaccc agtacttttt taaagtagcc tttggggttt gagatccact ttatttgcct
     4321 cagatacctt tgccccaaca aaccaacaaa actaaatact aaaaccagac cagctgcata
     4381 aaaaataaga ttgagtaaac caatccagaa actttgaggc aacacaaccg ccaagataac
     4441 aaaataagtt gggctg
//
LOCUS       DAOSWN010000056         4328 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_13871_length_4328_cov_4.914580, whole genome shotgun sequence.
ACCESSION   DAOSWN010000056 DAOSWN010000000
VERSION     DAOSWN010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13871_length_4328_cov_4.914580"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PLF29_02935"
     CDS             <1..534
                     /locus_tag="PLF29_02935"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HXK52916.1"
                     /translation="SVVKLSSVFSVVLLVLVAIYAVYMVYTVNQIKSATRQHEQNIAN
                     HRKDINSLKEIEILARSLDGRYKLLSEVLDKRQNYSILLEEITKRMPVTTRIDSWNIS
                     APGTVMISGVADDYLAIAKLLDNMSQKDFVDAGEGMESLLTDITLNSVQLDNQDTDVS
                     FALTVKYDSKLISGVRK"
     gene            536..1366
                     /gene="pilO"
                     /locus_tag="PLF29_02940"
     CDS             536..1366
                     /gene="pilO"
                     /locus_tag="PLF29_02940"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HXK52917.1"
                     /translation="MKKINIDTLAIKQMVANFLVPLISLGLVTAIGFLVLYPTISNRP
                     MYEEKLEASRALDEQLLTKLEKLNYLVDYKSAVDENNSLLSDVLAQKPLVPELLTQLD
                     IIAKEAGLGVEKLSYSFGAGKDTAEGTLTDINVNMGIIGTYDQLVAFFRALEKAGRLV
                     NVDNYRYTLTEDNALSINLTFSSPYVDVKTDAVTDDPIAFDISDTKFVSLMSSLKEFK
                     VYRISVDDYIEVPATPEIPTQQGEQPLDAPAEVGVLQQPGGAEAPAQVPVVPTEEPLN
                     "
     gene            complement(1496..2203)
                     /gene="trmD"
                     /locus_tag="PLF29_02945"
     CDS             complement(1496..2203)
                     /gene="trmD"
                     /locus_tag="PLF29_02945"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HXK52918.1"
                     /translation="MINFDVITVFPELINSFLKVLPYKKAIERELIKVNSINLRDFPL
                     DSYGTIDDKPYGGGIGMLLMIEPLFKAINKSIANYVPEEKNIIIVTSPQGETYNQKMA
                     RDLKNFNRITIVCGRYEGMDSRIFELNTILDKKVEIRAVSLGDFVLSGGELPALSIIE
                     SVSRLLPGVLEKEEAAEIESFTDDDMPEFPQYTRPQDFMGLKVPEVLLSGNHKEIEKW
                     RKEASKKLHIKKTQQKE"
     gene            complement(2258..2524)
                     /locus_tag="PLF29_02950"
     CDS             complement(2258..2524)
                     /locus_tag="PLF29_02950"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HXK52919.1"
                     /translation="MKEFLEYIVKNIVKHPDEMKIEETVEGSIVRILIKPAKEDMGVV
                     IGKEGRTINNIRALARAKAIKDNVKVYVDISEDGESEEAESTNS"
     gene            complement(2541..2975)
                     /gene="rpsP"
                     /locus_tag="PLF29_02955"
     CDS             complement(2541..2975)
                     /gene="rpsP"
                     /locus_tag="PLF29_02955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HXK52920.1"
                     /translation="MSVTIRLAKIGRKKAHAFKIVVSTTKDKRNGKYLDILGHYNPSV
                     KPVQYEIDKDKVEQWKSKGALVTDAVTALLNGEYEFKKYTRQNEQKSSESSATEGSNT
                     TKGAEDNKDQPEKDSMNSEAETKEEIAQELAETAEEATAAAE"
     gene            complement(2993..3685)
                     /gene="rnc"
                     /locus_tag="PLF29_02960"
     CDS             complement(2993..3685)
                     /gene="rnc"
                     /locus_tag="PLF29_02960"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HXK52921.1"
                     /translation="MTFNKEKFESIIETTIANEAVFEKAFTHRSYLNENPEINTSNER
                     LEFLGDAVLQLLSSEYLFKMYPNSPEGDLTNFRSAVVCTKSLAHDARRLGYGELLFLS
                     KGEETSGGRDREYILANTFEAVLGALYLDKGLDFCRQFTEKNLFYKVEQIVEDETYKD
                     AKSKFQETAQEKTGITPYYRVLESWGADHEKTFRVAVYLDEEQYGVGEGKSKQKAEQS
                     AAKNALDKLTSV"
     gene            complement(3719..3952)
                     /locus_tag="PLF29_02965"
     CDS             complement(3719..3952)
                     /locus_tag="PLF29_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52922.1"
                     /translation="MNYKKEIKELIADRAGVEIEEIHDDSYFEDDLNIGEFELIDILT
                     EIEDMYEIEDLLSRKQEFETVGELMDYLGDKLE"
     gene            complement(3989..>4328)
                     /gene="nusB"
                     /locus_tag="PLF29_02970"
     CDS             complement(3989..>4328)
                     /gene="nusB"
                     /locus_tag="PLF29_02970"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HXK52923.1"
                     /translation="ETAEDVLGEKDKSYDQRLLFEILNGVQKETDKIDGTISYCAPEW
                     PVDKISKIDLIILRISVFEILYNSNTPQKVAIDEAIELSKEFGNDTSSKFINGVLGAV
                     VDLEKKEVHN"
BASE COUNT         1149 a          872 c          769 g         1538 t
ORIGIN      
        1 agtgttgtta agctgagctc tgtattttct gttgtattac ttgttttagt ggctatatac
       61 gctgtgtata tggtttacac cgtaaatcaa attaaaagtg caaccagaca gcatgaacaa
      121 aatatcgcta atcacagaaa agacataaat tccctaaaag aaatagagat tctagcccgt
      181 tcgcttgacg gcagatataa gttgctatcg gaggttttgg ataagaggca gaattattct
      241 attttgcttg aggaaataac caagagaatg cccgtaacta caagaattga ttcttggaac
      301 atttcagcac ccgggacagt tatgatttca ggagtagcag acgattacct tgctattgca
      361 aagcttttgg ataatatgtc tcagaaagat tttgttgatg caggagaagg tatggagtct
      421 ttgttgactg acattacgtt aaactcggtt caattagata atcaggacac cgatgttagt
      481 tttgctctca ctgtaaaata cgattctaaa ttaatttcgg gagtgagaaa ataatatgaa
      541 aaaaattaac atagatacct tagcgataaa acaaatggtg gcaaactttt tagtgcctct
      601 gatttcttta ggccttgtaa ccgcaatagg atttttagta ctctatccta ctatctcgaa
      661 tagacctatg tacgaagaaa agctagaggc ttcaagggca ttagatgagc agttattaac
      721 gaagctcgag aaattaaatt atcttgtgga ctataagagt gccgtagatg agaataactc
      781 tctactttcg gatgttttag ctcaaaagcc tttagttccc gaacttctga cccagcttga
      841 tattattgca aaagaagcgg gcttgggcgt tgaaaaatta agttactctt ttggagccgg
      901 caaagatacc gccgaaggca ccttaacaga tataaatgtc aatatgggga ttattggaac
      961 ttacgatcaa cttgttgctt tttttagagc tttagaaaaa gccggacggc tggttaacgt
     1021 agataactac aggtacaccc ttaccgaaga taatgctttg tctataaatc ttactttttc
     1081 ttccccttac gttgatgtga aaaccgatgc agttactgat gatcctattg cttttgacat
     1141 ttctgacact aagtttgtgt cgttaatgtc atctcttaaa gaatttaagg tttacagaat
     1201 atctgtagat gactacatag aagttcctgc aacaccagaa attccgacac agcagggtga
     1261 gcagccttta gatgctccgg cagaagtcgg agtacttcaa cagcccggtg gtgccgaagc
     1321 tccggctcaa gtacctgtcg ttccgaccga agagcccctt aactagagca ttagctaata
     1381 gatgtagctg tagtttaccc aacatattgg gttttgcagc gcaaagcaat tacagtaaac
     1441 cgcctgcgta agctggtggt ggttgatagt acaggagatt gctgttatat agcagttatt
     1501 ccttctgttg cgttttcttt atatgtagtt ttttagaagc ttcttttcgc catttctcta
     1561 tttctttatg atttccgctt aaaagcacct ccggtacttt taagcccata aaatcttgcg
     1621 gtctggtata ttgaggaaat tcgggcatat cgtcatctgt gaaagactct atttccgcag
     1681 cctcttcttt ttcaagtacg cctggtaaaa gccttgagac gctttcaata attgaaagtg
     1741 ctggaagttc ccctcctgac aaaacaaagt cgcctaaaga gacagccctt atttcaactt
     1801 ttttatcaag tatggtgttt aattcaaaaa ttctactgtc cattccttcg tatcgcccgc
     1861 aaactatagt tattctatta aagtttttaa gatctcttgc cattttttgg ttgtatgtct
     1921 caccttgggg agaagtaact ataattatgt ttttttcttc cggtacgtaa tttgcaatag
     1981 atttatttat agccttgaaa agaggttcta tcatgagaag catgcctatt ccccctccat
     2041 agggtttatc gtctatagtt ccgtatgagt caagcgggaa gtctcttaaa tttatagaat
     2101 ttaccttaat tagttctctt tcgatagctt ttttatatgg taagactttt agaaaagagt
     2161 taataagctc gggaaataca gtaataacgt caaaattaat cattaattct tttgtttata
     2221 atttcgttac ttaaaactac tttcgtattg ttgaagatta gctatttgtg ctttcggcct
     2281 cttcgctttc accgtcttcg cttatatcga catatacttt aacattatct ttgattgcct
     2341 tcgctcttgc caacgctctt atattattta tggttcttcc ctcttttcct ataaccacgc
     2401 ccatgtcctc ttttgcaggt ttaattaaaa ttctgacaat cgagccttca accgtttcct
     2461 ctattttcat ttcatctggg tgttttacta tatttttgac aatatattct aaaaattctt
     2521 tcatatgctt tcagtcgcag ttattcggca gctgctgttg cttcctctgc tgtttcggcc
     2581 agctcctgcg caatttcctc tttagtttcc gcctcactgt tcatagaatc tttttcgggc
     2641 tgatccttat tatcttcggc cccttttgta gtgttagatc cttctgtggc agagctttcg
     2701 gatgattttt gttcattctg acgtgtatat tttttaaatt cgtattcgcc gtttaagagg
     2761 gctgtcacag cgtctgtaac tagcgctcct ttggatttcc actgttcaac cttatcttta
     2821 tcgatttcgt attgtaccgg ctttaccgaa ggattgtagt ggcctagaat atctaaatac
     2881 ttgccgtttc tcttgtcttt ggttgtagac acgacaatct taaatgcgtg tgcctttttt
     2941 ctgcctattt tagctaatct tattgtgact gacatagtac tagtggattg tatcagacag
     3001 aggtaagctt gtccaatgca tttttagctg cactttgctc tgctttttgc ttacttttcc
     3061 cctcccctac tccatactgt tcctcgtcta gatatactgc cactctgaat gttttttcat
     3121 ggtcagcgcc ccaagattcg agaactctgt agtatggagt aataccggtt ttctcttgtg
     3181 cggtctcttg aaatttagat ttagcgtctt tataggtctc atcttcaact atctgctcta
     3241 ctttgtaaaa taagtttttt tcagtaaatt gacggcaaaa atccaatcct ttatcaagat
     3301 atagagctcc aaggacggct tcaaatgtat ttgcaagtat atattctctg tctcgaccgc
     3361 ccgaagtttc ctcgcctttg gacaaaaata acaattctcc gtaaccaagt cttctggcat
     3421 cgtgggcaag agattttgta cacactaccg cggacctgaa gtttgttaga tctccctccg
     3481 gagaattcgg gtacatctta aacagatatt ctgaagatag caattggagt accgcatcgc
     3541 ctaaaaattc cagtctttcg ttggaagtgt taatttctgg attttcgttt aaataagacc
     3601 tgtgtgtaaa tgctttttca aaaacggctt cgttcgctat tgttgtttcg attatacttt
     3661 caaatttttc tttattaaaa gtcataaaaa tagtttcact ataagaactg tgctttgact
     3721 attctaattt gtctcctaaa taatccatca gctcccctac tgtttcaaac tcttgttttc
     3781 tgcttaataa atcttctatc tcatacatat cttcgatttc ggtcagaata tcaatcagct
     3841 caaattcccc aatatttaaa tcgtcttcaa aataggagtc gtcatgtatc tcttcaattt
     3901 cgactcctgc cctgtctgct attaactctt taatttcttt tttataattc atattattct
     3961 gtttataatc tatctcggtg tgttccgatt aattatgaac ctctttcttt tctaaatcta
     4021 ctactgcacc taatacccca ttaataaatt tactggatgt atcatttccg aactccttgc
     4081 taagttcaat tgcttcgtca atagcaacct tttgtggcgt gttgctgttg taaaggatct
     4141 caaacacaga aattcgcagg attattaaat caatttttga tattttgtca accggccatt
     4201 cgggagcaca gtatgaaatt gtcccatcga ttttatctgt ttctttctgt acgccattta
     4261 atatctcaaa aagaagccgc tggtcataag atttatcttt ttccccaagc acatcctcgg
     4321 ccgtctct
//
LOCUS       DAOSWN010000057         4154 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_14751_length_4154_cov_5.051964, whole genome shotgun sequence.
ACCESSION   DAOSWN010000057 DAOSWN010000000
VERSION     DAOSWN010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14751_length_4154_cov_5.051964"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="PLF29_02975"
     CDS             <1..603
                     /locus_tag="PLF29_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52924.1"
                     /translation="VGRVGALYFERLKTKKTYKYFDLPDVIKDQNLTEGLFEYLSRYD
                     AIIVHHGRYENILNQEPVSSSISGDALRDRVVGEGAEEGLNLKNASDEKIAERKEKSN
                     TLVTSRRRKLPETRYLFEPSLLEVVKYFEFSILELLFNQAIQESNLSKYASRMIGLNA
                     SSDRIQKKLGSLHNSLMFEKHRAESKKQLNKLSAVYGRVR"
     gene            600..2057
                     /locus_tag="PLF29_02980"
     CDS             600..2057
                     /locus_tag="PLF29_02980"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012236.2"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HXK52925.1"
                     /translation="MKSIDLEKNSLVGVVSAVNGFVAEIIFPSGKVPKIHTMLENTEL
                     GVVLQVSKIINQKTALCICLKHHTKIYRSLELTVTGEMLKVPVGKFTLGKSINLFGDY
                     VYFDSKVSEPEKEKILSGVADSSVSEYADVFSLPLNYSFVVNSSEVIETGIKVVDLFT
                     PLVKGRRVGLFGGAGVGKTILLNEILHNIIQKNKQNTLSVYAGIGERIREGHEVFNNL
                     GRTEIMSNVAMLFGTMADNPAVRYLSGLAAVANAEYFRDVLKKDVLFFIDNAFRFVQA
                     GNELSLFLNELPSEDGYQPSLYSQLAALHERLASTKDASITTIEAVYVPADDILDRAV
                     QSIFEFLDSSIVLSRDIYSEGVFPAVDILESNSSLLSPEIVGEDHYSTTLSAQRLLKT
                     AGDLDRIVSLVGEAELSFEDKLIYKRARKLKNYFTQNFFTSSQQTGRPGAYVPLADTI
                     RDTKAIISGQYDEITEDKFLFIASYSGSAEEENKE"
     gene            2054..2293
                     /locus_tag="PLF29_02985"
     CDS             2054..2293
                     /locus_tag="PLF29_02985"
                     /inference="COORDINATES: protein motif:HMM:NF014838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52926.1"
                     /translation="MISVQIVSKKRLIYQGKATSVTSKNRVGEFDILEQHANFITLME
                     DYVILNHKTNAEQVYELKSGLLVAENNKVAIFVEE"
     gene            complement(2334..2987)
                     /locus_tag="PLF29_02990"
     CDS             complement(2334..2987)
                     /locus_tag="PLF29_02990"
                     /inference="COORDINATES: protein motif:HMM:NF012890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HXK52927.1"
                     /translation="MPKIQKKDRKILNIKEAIDFIKANSKGNFDATIEVHANLGINHK
                     KQEQTVRTTTVLPHGTGKTVKVAVFSDKTVKEAELNLTEHDIERLEKGEIKPKIDFDV
                     LISEPKFMAKLAKAAKILGPAGVMPSPKAGTVTDDVEKAVSQVKGGKIEIRNEINAPI
                     IHTIIGKRSFDTSKLVENFEEVMSTIRNHRPQKVKPEAYIVSVFISSTMGPSVQIQL"
     gene            complement(2980..3426)
                     /gene="rplK"
                     /locus_tag="PLF29_02995"
     CDS             complement(2980..3426)
                     /gene="rplK"
                     /locus_tag="PLF29_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HXK52928.1"
                     /translation="MAEKKKKGKKIKGYIKLNIPAAQATPAPPIGPALGQHGVPLMDF
                     CKEFNARTASMAGNIIPVVITVFEDKSFTFITKTPVTSDLIKKALKVQKGAANNIKEK
                     VGTLSKSQIEDIAKTKMPDLNTDSLEEAVKIIEGTARSMGVKVENA"
     gene            complement(3436..4005)
                     /gene="nusG"
                     /locus_tag="PLF29_03000"
     CDS             complement(3436..4005)
                     /gene="nusG"
                     /locus_tag="PLF29_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HXK52929.1"
                     /translation="MTDNGNIYVISDHENPMAKWYVVHTYSGHEKKVAINLKQRAESL
                     GALDKILKVLIPQQQKIVVSEGKKRTIEERLYPGYILVHMDMNDSTWTVVRGTPGVTG
                     FVGMGTNPTPLPEKEVQALLKYIKTEQPKFEAKFSEGDTIKIVDGPFSEFLGKVEEVS
                     EDQGKLKVLVSVFGRETPLELDFTQVKSI"
     gene            complement(4002..>4154)
                     /gene="secE"
                     /locus_tag="PLF29_03005"
     CDS             complement(4002..>4154)
                     /gene="secE"
                     /locus_tag="PLF29_03005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HXK52930.1"
                     /translation="GSYTEFKKVEWPSRKQTLLLTAYVIGVSLVVAFFVASADLVFKE
                     FIKVLL"
BASE COUNT         1139 a          758 c          840 g         1417 t
ORIGIN      
        1 gttggaagag tgggggcatt atattttgag agactgaaaa caaagaaaac ttacaaatat
       61 tttgatttgc ctgatgttat aaaagatcag aatctaactg aaggtttgtt tgagtatctt
      121 tcaagatatg atgctattat tgtgcatcac ggtcgatacg aaaatattct aaatcaagag
      181 ccggttagtt cctcgatatc gggggatgct cttcgagatc gggttgtagg agagggagca
      241 gaagaaggcc tgaatttaaa aaatgcctcc gatgaaaaaa tagccgaacg gaaagagaag
      301 agtaacacgt tagtaacttc ccgaagacgg aagcttccag aaacacgata tctttttgag
      361 ccgtctttat tagaggttgt aaaatatttt gaattttcta tacttgagct tctatttaat
      421 caggcaatac aagagtctaa tttaagtaaa tacgcctcta ggatgatagg gttaaacgca
      481 tcttctgata gaattcaaaa gaagcttggt agtttacata attctctaat gtttgagaaa
      541 catcgagccg aatcaaagaa gcaacttaat aaactttcag cggtatatgg gagggttaga
      601 tgaaaagtat tgatttagaa aaaaatagtc tagtgggcgt ggttagtgcg gttaacggtt
      661 ttgttgctga aatcatattt ccttcgggaa aggtgcctaa aatacatact atgcttgaga
      721 ataccgagtt gggtgtagtg ttgcaggtta gtaaaattat taatcaaaaa acagctttat
      781 gcatttgttt aaaacatcac acaaaaatct accgatcttt ggaattaact gtaaccggtg
      841 agatgttgaa agtgccggtt ggaaaattta ctttagggaa gtcaatcaat ctttttgggg
      901 attatgtgta ttttgatagt aaagtatccg aacctgaaaa agaaaagatt ttgtccggtg
      961 tagctgacag ttctgtatct gaatatgccg atgttttttc tcttcccttg aactacagct
     1021 ttgtagttaa tagtagtgaa gtaatcgaaa ccggcattaa agtcgttgat ttattcaccc
     1081 ctctggttaa gggtagaagg gtaggtcttt ttggcggcgc cggtgtagga aagaccatac
     1141 ttctaaatga aatcttgcac aacattattc agaaaaacaa gcaaaatacc ctatcggtat
     1201 atgcgggtat tggggagcga attagagaag gccatgaagt gttcaataac ttaggtcgca
     1261 ctgaaattat gtccaatgtt gctatgcttt ttggaacgat ggccgataat cctgcagtaa
     1321 ggtacttatc gggcttggcg gctgttgcta acgctgagta ttttagagat gtcttgaaaa
     1381 aagatgtatt gttttttatt gataatgctt ttagatttgt tcaggccggg aacgaattgt
     1441 ctcttttttt gaatgaactt ccttcggaag atgggtatca gccttcttta tattcacagc
     1501 tcgcggcttt gcatgaacgc cttgcttcaa caaaagacgc ctctataacc acaatcgagg
     1561 ctgtgtatgt tcctgccgac gatattttag atcgtgccgt acagtccatt tttgaatttc
     1621 ttgattcttc catagtacta tccagagata tttattcaga aggtgtgttt ccggctgtag
     1681 atattttaga gtctaactca tctctgttga gcccggagat tgtgggagaa gatcattact
     1741 ccacgacttt atcagcccag cggctgctta agactgccgg agacttggat cgaatcgtgt
     1801 ctcttgttgg agaggccgag ctatcattcg aggacaaatt aatttacaaa cgcgcccgta
     1861 aattgaagaa ctactttaca cagaacttct ttacctcttc tcagcaaaca ggacgcccgg
     1921 gtgcttatgt tcctttggca gacacaataa gagacactaa ggcaataatc tcaggtcaat
     1981 acgatgagat aactgaagat aagtttctat ttatagcctc ttatagcggt agtgctgaag
     2041 aggaaaataa agaatgattt cggttcagat agtttctaaa aaaagactta tatatcaagg
     2101 aaaggcgact tctgttactt caaaaaatag agtgggggag tttgatatat tagaacagca
     2161 cgcgaacttt ataactttga tggaagatta cgttattttg aaccataaaa ctaacgccga
     2221 acaggtttac gaacttaagt cgggtttgct tgttgcagaa aataacaaag tggctatttt
     2281 tgtcgaagaa tgaggtaagt aagtatctcc ttacgggccc tgctttagct atattatagc
     2341 tgaatctgta ccgagggccc cattgtggaa cttatgaaga cagatactat gtaggcttcc
     2401 ggttttactt tttgtggcct gtggtttctt attgtactca tgacctcttc aaagttctct
     2461 actaactttg atgtatcaaa agaccttttt ccaattattg tgtgtattat aggagcgttt
     2521 atttcgtttc ttatttcaat ttttcctccc ttaacttggg aaaccgcttt ttcgacatcg
     2581 tcggtgactg ttcctgcttt tgggctaggc atcacgcctg ccgggccaag aatcttggcc
     2641 gctttggcta gcttggccat aaattttggc tctgatatta gtacatcaaa atctattttg
     2701 ggttttattt ctcctttttc cagcctttca atgtcgtgtt ccgttaaatt taactcagct
     2761 tcttttacgg ttttatcaga aaaaacagct acttttactg tttttcctgt cccgtgaggt
     2821 agcacggttg ttgttctaac agtttgttct tgtttcttgt ggttaatacc aaggtttgca
     2881 tgaacttcta tagttgcgtc gaagttaccc tttgaattag cttttataaa atctatggcc
     2941 tcttttatat tcaatatttt tctatctttt ttttgtattt taggcatttt ctactttaac
     3001 tcccatagat ctggcagtac cctcaataat ttttacagct tcttccaaag agtctgtgtt
     3061 aagatcgggc attttagttt tggcaatatc ttcaatctga gattttgata aagttcccac
     3121 tttctcttta atgttgtttg ctgctccctt ttgaactttt aatgcttttt taattaagtc
     3181 tgatgtaacc ggggtcttgg taataaaggt aaaagattta tcttcaaaca cagtaataac
     3241 aacaggaatt atatttcctg ccattgatgc tgttctggcg ttaaactctt tgcagaagtc
     3301 cattaaagga actccatgct ggcctaaagc agggccaatt ggaggtgccg gtgttgcttg
     3361 ggctgccgga atatttaact ttatgtaccc tttaattttt tttccttttt tctttttttc
     3421 tgccatagct accctttata tacttttaac ctgagtaaag tcaagttcaa gaggtgtttc
     3481 tcttccaaaa actgatacta acacttttaa tttgccctga tcttcgctca cctcttctac
     3541 ttttccaaga aattccgaga aagggccgtc aactattttt atggtgtctc cctcggagaa
     3601 tttagcttca aatttgggct gctctgtttt tatatacttt agtaatgcct gaacctcttt
     3661 ttctggtagg ggagtaggat tagtgcccat tccaacgaaa cctgtcactc cgggggttcc
     3721 cctaactact gtccaagtgc tatcgttcat gtccatgtgt actaggatat atccggggta
     3781 gagccgttct tctatcgtgc gtttttttcc ttctgatacc actatttttt gttgttgagg
     3841 tattaggact tttagtattt tatctaaagc tccaagagat tctgctcttt gctttagatt
     3901 tatagctact ttcttttcgt gtcccgagta ggtatgtact acataccatt tagccatcgg
     3961 gttctcgtgg tccgagatta catatatatt tccattatct gtcatagtaa aaccttaata
     4021 aattctttaa aaactaaatc tgcactagcg acgaaaaacg ccaccaccaa gctaacacct
     4081 attacgtatg cagtcaatag caaggtctgt ttccgacttg gccattccac ttttttaaac
     4141 tctgtatacg agcc
//
LOCUS       DAOSWN010000058         4140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_14829_length_4140_cov_4.030110, whole genome shotgun sequence.
ACCESSION   DAOSWN010000058 DAOSWN010000000
VERSION     DAOSWN010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14829_length_4140_cov_4.030110"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /gene="rplP"
                     /locus_tag="PLF29_03010"
     CDS             complement(<1..140)
                     /gene="rplP"
                     /locus_tag="PLF29_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007233541.1"
                     /note="located in the peptidyl transferase center and may
                     be involved in peptidyl transferase activity; similar to
                     archaeal L10e; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HXK52931.1"
                     /translation="MLMPKKVKFRRSMRGNNKGLAVRGSTISFGEYALKADDRCLITS
                     RQ"
     gene            complement(143..775)
                     /gene="rpsC"
                     /locus_tag="PLF29_03015"
     CDS             complement(143..775)
                     /gene="rpsC"
                     /locus_tag="PLF29_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HXK52932.1"
                     /translation="MAQKIHPYGFRLGITKDWKSRWTTDKKGFGKLVVEDKVIRDFLD
                     KKLGNAGLESVEIERSHNEVSLIIRVSKPGLVIGRGGSGVEELQKEIKKLTNAKVKLT
                     VEEVKNAEANARLVADYISRQLKRRMSYRRVCMAATTSAMDKGAKGVKIKISGLLSGG
                     NSIARSDVFSRGPVPRQTLRADIDYAQVTCHLIYGTIGIKVWIYNGEVEA"
     gene            complement(777..1127)
                     /gene="rplV"
                     /locus_tag="PLF29_03020"
     CDS             complement(777..1127)
                     /gene="rplV"
                     /locus_tag="PLF29_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008211812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HXK52933.1"
                     /translation="MSNAIVYAKHKYAKSSVKKVNPVLALIRGKNVAEAKRVLMFNRT
                     NASTLILKVLDSAVANARHNLDLSEKSLVVSESYVNEGPMRKTGRPQARGRFSPIFKR
                     SCHIVVGLDERKSE"
     gene            complement(1130..1399)
                     /gene="rpsS"
                     /locus_tag="PLF29_03025"
     CDS             complement(1130..1399)
                     /gene="rpsS"
                     /locus_tag="PLF29_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HXK52934.1"
                     /translation="MSRSLKKGPYVDQKLMEKVAKIKAAGKKGPIITWARASTITPEM
                     VGMVIAVHNGKKHIEVSISEAMVGHKLGEFSPTRTFRGHSSKKKK"
     gene            complement(1399..2232)
                     /gene="rplB"
                     /locus_tag="PLF29_03030"
     CDS             complement(1399..2232)
                     /gene="rplB"
                     /locus_tag="PLF29_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HXK52935.1"
                     /translation="MLKKYKPTSPGIRTRKTLVKSNITKSKPEKRLTKSIKGHVGRSK
                     GTVTSRFRERGAKKHYRIIEFKRIKFGIPAKVAAIEYDPNRGPDLALLHYADGEKSYI
                     LAPEGLEVGMVVEVGEDAKAQVGNSLPLSNIPAGMPIHNIELNPGKGAQMVRGAGNSA
                     VVLAKEGNFVNVKLPSGEVKKVAAKCYATIGTLGNADHRLARLGKAGKMRHLGRRPHV
                     RGVAMSNPKDHPHAGSYRDNGTGHPKTPWGKPTRGFKTRSRTHTNKFVVTARPRKRKG
                     K"
     gene            complement(2234..2527)
                     /gene="rplW"
                     /locus_tag="PLF29_03035"
     CDS             complement(2234..2527)
                     /gene="rplW"
                     /locus_tag="PLF29_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HXK52936.1"
                     /translation="MKLNEIIKKPIITEKSVQAEGQNKYTFEVGVKASKGAIAEVAAK
                     MFGVEVLDVKTYIMPGKKRRILKSRRFTKTPKWKKAVIEIKKGQKIKLTEETK"
     gene            complement(2527..3162)
                     /gene="rplD"
                     /locus_tag="PLF29_03040"
     CDS             complement(2527..3162)
                     /gene="rplD"
                     /locus_tag="PLF29_03040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HXK52937.1"
                     /translation="MQINVINTKGEVLEKMDLDTSVFKLEPRIETLKQYVRVFLHNMR
                     QGTSSTKTRAEVSGAGRKPWRQKGTGRARVGSIRSPLWRKGGIVHGPKPKSWSLKLTK
                     KMKSLALLSALSLKVSKDNVFVVDTLKAAEPSTSKFAAMMKAAGLDSRRSLIILDDND
                     MNVRKSVANIPWVHTALVQNVNAYELMNAHKVIFTKKAVDIIQTKYKGRFK"
     gene            complement(3152..3637)
                     /gene="rplC"
                     /locus_tag="PLF29_03045"
     CDS             complement(3152..3637)
                     /gene="rplC"
                     /locus_tag="PLF29_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HXK52938.1"
                     /translation="MQDYKNWNKLKGKKLNMSQVFTPEGKVIPVTYVSLESALDSSVL
                     NNEVVISSISKGKGFTGVMKKWNFKGAQATRGQSDKARSGGSIGAQTPSKVFKGKKMA
                     GNQGNKQVTLKGLRIVDINNETKIIALTGPVPGARNADITIYIDLPQQGTVVEEVQNA
                     N"
     gene            complement(3859..>4140)
                     /gene="rpsJ"
                     /locus_tag="PLF29_03050"
     CDS             complement(3859..>4140)
                     /gene="rpsJ"
                     /locus_tag="PLF29_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HXK52939.1"
                     /translation="LKAYDSRIIDLSCQKIADATVRTGASIVGPVPLPTKTERYTVIR
                     GPHIDKRSREQFELRTHKRVLDVVNPTAATIETLSNLSLPAGVGISIKM"
BASE COUNT          998 a          959 c          678 g         1505 t
ORIGIN      
        1 atctgccttg aagttattaa acatctatca tcggctttta aagcatactc accaaaggat
       61 atagtactac ctctaacggc gaggcctttg ttgtttccac gcatacttct tctaaatttt
      121 acttttttag gcatcaacat gtttaagcct ccacctcacc attataaatc catactttaa
      181 tacctatggt tccgtatatc aaatggcaag taacctgtgc ataatcaata tcagctctta
      241 aagtctgcct tggaaccggg cctcgtgaaa atacatcaga tctggctata gagttacctc
      301 cgcttaataa gccggatatt ttgatcttaa cgccctttgc ccccttatcc atagcggacg
      361 ttgtggccgc catacatact ctcctgtagg acattctgcg ttttaactga cgagatatat
      421 agtctgcaac taatcttgcg tttgcctcag cgttttttac ctcttcaaca gttaatttaa
      481 cttttgcgtt agttaacttt tttatctctt tctgaagttc ttctactccg gaacctcctc
      541 tacctataac taatcccggt ttagagactc ttataataag acttacctcg ttgtgcgacc
      601 tttcgatttc tacgctttcc aatcctgcat taccaagttt tttgtctaaa aagtcacgaa
      661 taaccttatc ttctaccact agttttccga aacctttttt atcggtagtc cagcgagatt
      721 tccaatcttt tgttattcct aatctaaatc cgtaaggatg aatcttttgt gccatattat
      781 tcagacttcc tttcgtctaa gcctactact atgtggcaag atctcttaaa tatagggcta
      841 aaccttcccc ttgcctgagg tcttccggtt tttctcattg gaccttcgtt tacatatgat
      901 tctgaaacaa ccaaagactt ctcacttaaa tcgagattgt gtctggcatt agcaactgcg
      961 gaatcaagca cttttagtat taaagtcgaa gcatttgttc gattaaacat tagtactctt
     1021 tttgcctcag ctacgttttt gccccttatc agtgctaaga cgggattcac cttcttaaca
     1081 gatgacttag cgtatttatg ttttgcatag actattgcgt tactcatttt tatttcttct
     1141 tcttagagga atgccctcta aatgtccttg taggcgaaaa ttcgcccaac ttatgcccca
     1201 ccattgcttc cgaaatggac acttcaatgt gctttttgcc attgtgtact gctataacca
     1261 tgcctaccat ctcaggtgtt atagttgacg ctcgtgccca tgtaattata gggccctttt
     1321 taccggccgc ttttatttta gcgacttttt ccattagttt ttggtcaacg taaggacctt
     1381 ttttaagcga tcttgacatt atttaccctt tctctttctt ggtcgagctg ttactacaaa
     1441 cttattagtg tgtgtacggc tacgtgtctt aaaccctctt gtaggttttc cccaaggagt
     1501 ctttggatga cctgtaccgt tatctctgta agaacctgca tgtgggtggt cttttggatt
     1561 cgacatagcg acacctcgta catgtggtct tctacccaaa tgtctcattt tacctgcttt
     1621 tcctagtctt gcaagtctat ggtcagcatt cccgagggtt cctattgtag catagcattt
     1681 tgcagcaact tttttaacct ctccactagg gagtttaacg tttacgaaat ttccttcttt
     1741 ggccaaaaca acagcgctgt tacctgcgcc ccttaccatc tgtgcacctt ttcccgggtt
     1801 tagctcaata ttatgtatag gcatacctgc aggaatgtta cttaacggta agctatttcc
     1861 aacctgagct tttgcatctt cccctacctc aacgaccatc ccgacttcta aaccttcggg
     1921 tgccaaaata taagacttct ccccatccgc gtagtgtaaa agggctaaat cgggccctcg
     1981 gttcggatca tattcgattg cggccacttt tgcaggaatg ccgaatttta ttcttttaaa
     2041 ttctattatt ctgtaatgct tttttgcgcc tctttctctg aagcgacttg taacagtgcc
     2101 tttactacgt ccaacatggc cttttattga tttcgttaat cttttttcag gctttgattt
     2161 agttatatta ctctttacga gtgtttttct tgtcctaatt cccggacttg taggcttata
     2221 tttttttagc attttacttc gtctcctccg tcagttttat cttttgccct tttttaatct
     2281 cgattaccgc tttcttccac tttggggttt tggtaaatct tctggatttc agtattcttc
     2341 tcttttttcc gggcataata tatgttttta catccaaaac ttcaacacca aacatttttg
     2401 ccgccacttc agcaattgca cctttggagg ctttcacacc cacttcgaaa gtgtatttgt
     2461 tctgcccctc ggcttgtaca gacttttcgg ttattatcgg ttttttaatt atttcgttta
     2521 acttcattat ttaaatctcc ctttgtattt tgtctgtatt atatcaacgg ccttttttgt
     2581 aaaaataact ttgtgggcat tcataagttc ataagcatta acattttgta ccaaagcagt
     2641 atgaacccaa ggaatattgg cgacactctt tctcacattc atatcattat catctagtat
     2701 tattaaggat cttcttgagt ctaaaccggc agccttcatc attgctgcaa atttagaagt
     2761 acttggctct gctgccttta gcgtatccac aacaaaaacg ttatccttag acaccttcaa
     2821 agatagtgcg cttagaagtg ctaaagattt catcttcttt gtaagtttta agctccacga
     2881 ttttggctta ggtccgtgca caattccacc ttttctccat agagggcttc tgatagaccc
     2941 gactctagcg cgtcccgtac ctttttgacg ccaaggcttt cgaccagccc cggatacttc
     3001 agctctggtt ttagttgatg aagttccctg tcgcatatta tgcaaaaaga ctcttacata
     3061 ctgttttaaa gtctctatac gaggctcaag tttaaacaca gaagtatcaa gatccatttt
     3121 ctctaaaact tcaccctttg tattaattac attaatttgc attctgtacc tcctcaacga
     3181 ctgttccttg ttgcggtaaa tctatgtaaa tcgttatatc tgcgtttctc gctcccggaa
     3241 ccgggcccgt taaagctatg attttggttt cattattaat atcaacaatt cgcaggcctt
     3301 tcaaagtaac ctgcttgttg ccttggtttc cggccatctt cttaccctta aaaaccttgc
     3361 ttggtgtctg agctccaata gagccgccgg atcttgcctt gtctgactga cctcgggtag
     3421 cctgggcacc tttaaaattc cattttttca taactcctgt aaatccttta cctttggaga
     3481 tgctggaaat tacaacttca ttatttaaaa ccgagctgtc taaagcagac tccaacgaaa
     3541 catacgtaac gggaataact ttaccttcag gtgtaaatac ctgactcatg ttaagtttct
     3601 taccttttaa tttgttccaa ttcttatagt cttgcatttt atctcgctaa cttcgcttat
     3661 tactaagcat tggctctaag ctctatttta aacttttcag tttgttttta gtaataaatg
     3721 ccgagctaaa agcatttata ttatttaaat aaaaaaagcc ctaaagggct cctaatactt
     3781 tatattattt gccctgttat gtaaaccttt ttattaaaat caacattttt tattttgcgc
     3841 agtaggcgca tttggcagct acattttaat agaaatacct acacctgccg gtaagctaag
     3901 gtttgataag gtttctattg ttgcagctgt tggattgact acatccaaca ctctcttgtg
     3961 agttctaagt tcaaactgct ctcgagacct tttatctatg tgggggcctc ttattacagt
     4021 gtatcgttct gtttttgttg gcaaaggaac aggtcctact atcgacgctc cggttcgaac
     4081 cgttgcatcg gcaatttttt ggcaactcaa atcaatgatt ctgctatcgt atgcctttag
//
LOCUS       DAOSWN010000059         4069 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_15227_length_4069_cov_4.919283, whole genome shotgun sequence.
ACCESSION   DAOSWN010000059 DAOSWN010000000
VERSION     DAOSWN010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15227_length_4069_cov_4.919283"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..376)
                     /locus_tag="PLF29_03055"
     CDS             complement(77..376)
                     /locus_tag="PLF29_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXK52940.1"
                     /translation="MANLIRLSKAFADKNRVKILQMLSEGPLNVSTVADDLNVEENLA
                     SHHLRVLHSLGLLKNTKRGRQVFYSLNKAKMVSLIRDLREVPFFKEVLEEALTKE"
     gene            complement(398..2044)
                     /locus_tag="PLF29_03060"
     CDS             complement(398..2044)
                     /locus_tag="PLF29_03060"
                     /inference="COORDINATES: protein motif:HMM:NF013260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK52941.1"
                     /translation="MPNKILSVVASGSELVRVEIEVVVKKRGFPSFELVGLVGKDAVE
                     CKKRILCAFESAGIRIRNMKVLVNLRPSNVVKSGAHFDLAISAGILLAIKKLTLNRSL
                     LLVGEVSLSGHFIPSSYDVLVKMQHVLNEEHVLIVAGEIGLKRHDSVQNFYFVESIED
                     LGVFLESLSRYQDFASLPPAVEKKGMDIFGSLLCYDSEEDSLYRTAEKSFDSILGNEK
                     IKRALEISAVGRHSLYVYGPPGSGKTTLLNSLVFLLPKMEPKEALRSSILYGLENLSS
                     ASSDRAGVTLMPPCRILSHTTSVSSLTGKSNSRSIGELSLAHNGVLIMNELNLFSSKV
                     SDLIKEVLDDKAFYVIKDRRLVKVPSEFILLASSNLCPCGYYGSEIKKCTCLPFQVRQ
                     FNSKISGAFLDRFDMTVYAEQVTDTSENYSSEKVMAEDKKLVTNLPEKDSYKFEQLKN
                     KIFSAVEYRKNRLLSFPDSAQINNKNIYTPEIFFQKSKITLEAKIFALSILKSNSLSM
                     RGFSKFLALSRTLADIDSDEKIRVQHVAEAISFKEAVALQ"
     gene            complement(2034..2885)
                     /locus_tag="PLF29_03065"
     CDS             complement(2034..2885)
                     /locus_tag="PLF29_03065"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HXK52942.1"
                     /translation="MCSFITLGSPFYPQGIRLQKNPPFKLYYIGDLNTAIGNKIISIV
                     GSRKMTKYANMVLERFIPVFVRSGLVVVSGFMHGVDIKAHELVVSSGGRTIAILPCGL
                     DINYPSDFAYLRDAIVKGGGLLLSRFSEGERPQRWMYIKRNELVASISECVLVIEADA
                     GSGTMTTANIALKIKKPVFVIPSSINSPNSSGVVELLQKGAHAVNDPLDILKFFKVAY
                     ENTKNVPVEIEGDQLDVFLYLQTKPSNAYALSKGLRLELSSVYIVLHELLARGLVYLD
                     KGKYHAK"
     gene            3084..3788
                     /locus_tag="PLF29_03070"
     CDS             3084..3788
                     /locus_tag="PLF29_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52943.1"
                     /translation="MKKKKITKNNNLLSVDKIIQAAKKEGIDFGKTDPYNRLRYYTKI
                     GWLPHMIRKKTERGEIAGHYPLEVVETLKIIQDLKNSGATNEQINSKLHNTQTNQNIK
                     QSIRMLTVKDSVTSYALLGLIIVLIILQITQLKKKDPKNVILYANDDLSSANQNYDYA
                     NVTSGVAYIPRGSKSITVKNTNINELSKIYITFNDDYSPASKYWVSSKTAGEGFTIET
                     DVPVKADSEFNWWVSN"
     gene            complement(3800..3876)
                     /locus_tag="PLF29_03075"
     tRNA            complement(3800..3876)
                     /locus_tag="PLF29_03075"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3840..3842),aa:Ala,seq:ggc)
BASE COUNT         1381 a          844 c          701 g         1143 t
ORIGIN      
        1 cctgttgggt aaactacatt ttctagctta cgcaagtagt tttaagtaac gaaattataa
       61 aaaaaggttt gaaaaattac tcttttgtta acgcctcttc caaaacttct ttgaaaaaag
      121 gtacttcacg aagatctcta attaaagaaa ccatttttgc tttatttaga gaataaaaaa
      181 cctgtcttcc tctttttgtg ttttttaata gaccgagaga atgtaaaact cttaaatgat
      241 gggaggctaa gttttcttct acatttaggt cgtctgctac tgtacttaca tttaaagggc
      301 cttcgctgag catttgtagt atctttactc tatttttgtc agcaaaagct tttgataatc
      361 ttatgagatt ggccataaac aaaatataca ataacaatta ttgcaatgca actgcctctt
      421 taaaagagat ggcttccgcc acatgctgaa ctctaatctt ttcatcactg tctatatctg
      481 ctaaggttct agataaggct aaaaacttgc tgaagcctct cattgaaagt gaattcgatt
      541 ttaaaatact caatgcgaaa atcttggctt ccaaagttat tttacttttc tggaaaaaga
      601 tttcaggggt atatatattt ttattgttta tttgagcaga gtctggaaaa ctcaatagtc
      661 tgttttttct gtattcaacc gcagaaaata ttttattttt taactgttca aatttgtacg
      721 aatctttttc tgggaggttc gtgactaatt ttttatcttc agccataact ttttcgctag
      781 aataattttc ggacgtatcc gtaacttgct cagcataaac agtcatatca aacctatcta
      841 aaaatgctcc tgaaattttg gaattaaatt gtcttacctg aaaaggcaag caggtacatt
      901 tttttatttc gctaccataa taaccgcacg gacataaatt acttgaggcg agcaatataa
      961 actcagacgg tactttaacc aagcgcctat ctttaatcac ataaaaagct ttatcatcta
     1021 acacttcctt gatgagatct gaaaccttcg aagaaaataa atttaactca ttcataatta
     1081 aaaccccatt atgggccaga gaaagctccc caatactgcg gctatttgac tttccggtta
     1141 aacttgagac tgaagtagtg tgtgaaagta tccggcacgg cggcataaga gtaaccccag
     1201 cacggtcaga agaagcactc gacaaatttt ccaaaccata aagaatggag gaccttaggg
     1261 cctccttggg ttccatttta ggtaacaaaa aaacaagtga attaagtagt gtagttttac
     1321 ctgaacccgg agggccataa acatataaac tatgtctgcc gacagccgat atttcaagag
     1381 cccgtttgat cttctcattg cccaatatag aatcaaaact tttttctgcg gtacgataca
     1441 aagaatcttc ttcggaatca tagcaaagca gagatccaaa aatatccatc cccttttttt
     1501 ctacagcagg cggaagggat gcaaaatctt ggtaacgcga aagtgattct aaaaacactc
     1561 caagatcttc tatagattct acaaaataaa agttttgaac cgagtcatgc ctttttaacc
     1621 caatctcgcc ggcaacaatt agaacatgct cctcattaag gacgtgttgc attttaacca
     1681 ggacatcata gctactaggg ataaagtgtc cactaaggga tacctccccc accagcaaca
     1741 agcttcgatt tagtgtaagt tttttaatgg ctagcaatat acccgcagat attgctaaat
     1801 caaaatgtgc gccggatttt actacattag aaggacgtag atttactaaa accttcatat
     1861 ttctgattct tataccggcg ctttcaaagg cgcacaggat acgtttctta cattcaaccg
     1921 catctttgcc tacaaggcca acaagttcaa aggagggaaa gcctcgtttt ttaacaacta
     1981 cttcaatctc aacccgaacc agctcagagc cagaagctac aacagacaaa attttatttg
     2041 gcatggtact tccctttatc taagtacacg aggcctcgcg cgagtagttc atgcaatact
     2101 atgtaaaccg atgataactc aagtctcaat cctttcgata aagcataggc gttactaggc
     2161 tttgtttgta aatagaggaa aacatcaagc tgatccccct ctatttctac tggaacattt
     2221 tttgtatttt cataagcaac tttaaaaaat tttaggatgt ctaaagggtc gttaactgcg
     2281 tgggctcctt tttgcaacag ctcaacaact ccagatgaat taggagaatt tatagacgac
     2341 ggaattacaa aaacaggctt tttaattttt agagctatat tggcagtagt catagttcca
     2401 ctgcccgcat cagcctcaat gactaaaaca cactcggata tagatgctac aagctcgttg
     2461 cgcttgatgt acatccatcg ctgcggcctc tccccttcag aaaagcgcga taacagcaag
     2521 cccccacctt taacaatcgc gtctctgaga tacgcaaaat cagaaggata attaatatct
     2581 aaaccacaag gcaaaattgc tatggtccga ccaccgcttg aaacaacgag ttcgtgagct
     2641 ttaatatcta caccatgcat aaagccagaa accacaacaa gccccgaccg aacaaaaaca
     2701 ggaataaatc tttccaatac catgttcgca tactttgtca tctttctgct gcctactatg
     2761 cttatgatct tattgcctat tgccgtattc aaatctccga tgtaatatag cttaaaaggc
     2821 gggttttttt gcaaacgtat gccctgcggg taaaaaggac tgcctaaagt tataaagctg
     2881 cacataaaaa aatgatacca ggcaaaacca cgatttagct ttaagcgtat attcaaaata
     2941 gacgacactt tatgttagaa tataccaaaa cattcgagct aaaaacgaca gctactttag
     3001 aaagcaagaa aagatcaacg agaagtagac aatgcaataa acaagtagcc cctgaatatg
     3061 ttaacgcaca ataaagacaa tatatgaaaa agaaaaaaat aacaaaaaat aataatctac
     3121 tatcagtaga caagataata caggcagcta aaaaagaggg cattgatttt ggaaaaacag
     3181 acccatacaa caggctaaga tactacacga agatagggtg gctaccccac atgataagaa
     3241 aaaagacaga aaggggagaa atagcaggtc actatcccct agaagtcgtt gaaactttga
     3301 agattataca agatcttaaa aattccggag caacaaacga acagattaac agtaaattac
     3361 ataacacaca aacaaatcaa aacataaaac aaagcatcag aatgctaaca gttaaagatt
     3421 cggttacgtc atatgcattg cttggcttaa ttattgtcct tatcattctt caaataacac
     3481 agttaaaaaa gaaggaccct aaaaatgtta tattatacgc aaatgacgac ttatcctctg
     3541 caaatcaaaa ctatgactac gcaaatgtaa catcaggcgt ggcttatata ccaagaggtt
     3601 caaaatcaat tacagttaaa aacacaaata taaatgaact ttctaagata tacataacat
     3661 ttaacgacga ttactccccc gcttctaagt attgggtaag cagtaaaacg gcaggagaag
     3721 gctttactat tgaaacagac gttccggtta aagcggattc ggaatttaat tggtgggtgt
     3781 ctaattaata caagaggttt ggtggacctg aggggactcg aacccctagc ctcctcaatg
     3841 ccattgaggc gcgctaacca gttgcgccac aggcccacat actttcttct acgtactttt
     3901 tcaagctaag aagcgcaaag gaaatactta tctattatac acaattctct tatttggtaa
     3961 atctctaagg gtaagatcca gcccctcaag ccccagaaag cgggttgaga accggaccga
     4021 ctgcccctcg cggatccaga cctcctgacg gaagttgtcc gtttggacc
//
LOCUS       DAOSWN010000060         3819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_16745_length_3819_cov_3.597768, whole genome shotgun sequence.
ACCESSION   DAOSWN010000060 DAOSWN010000000
VERSION     DAOSWN010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16745_length_3819_cov_3.597768"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PLF29_03080"
     CDS             <1..420
                     /locus_tag="PLF29_03080"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXK52944.1"
                     /translation="DKVRGKYFLRKFVFVDEIGEAYSRADFVLCRAGAHTVAELLALR
                     KPAILIPIPWVSHNEQYENARYVQKFGLGVILDERNISPPVLLQEIKKFSENIKSYKY
                     NDESDLHIKLSSNAAIDIAGVILEVAKAQKRKDVKNV"
     gene            413..1228
                     /locus_tag="PLF29_03085"
     CDS             413..1228
                     /locus_tag="PLF29_03085"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HXK52945.1"
                     /translation="MSDIYLPKKMRKKRESSAAQRRNNSFIYAKRKAFMLFRRFTAVL
                     VSLTVICAFAVGVKKSILDEKFFNVSSYIVVGAGNYVNKDDVEQLVRANSFDQLIFRV
                     DVSGIESRLLEIFKGGRKFTITKEYPDKLKIEVVERKPIAIISNPGSEDFFIVDDEGY
                     LLGQVDRRAYDLPIISYNGELQVGYTLDAKVVPVYLELLKALEEKKVKATSLSITNDY
                     VRFYSENNVEVLVSIEKNIRTSVGILTALIRQLSLGGESFNKIDLRYDKVIVK"
     gene            1236..2489
                     /gene="ftsA"
                     /locus_tag="PLF29_03090"
     CDS             1236..2489
                     /gene="ftsA"
                     /locus_tag="PLF29_03090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HXK52946.1"
                     /translation="MPKERIITGIDIGSSKVSTIIASLNNGRLSVIGVSGSVPSKGIT
                     KGNVSDIDDTVESISKSIERAERMAGVSISSAFITLNGSHIKTQNSHGVVAISNPNSE
                     INEYDIARVTDAARAVTLPSSEEIIHVIPRDFKVDSQEGVKNPERMSGVRLEVETHII
                     YGLASAIKNLTKCVQQVSIDVTALVFTALAASEATLTDTERELGTLLIDIGGGTTSVI
                     AFNDGSPIFSSVLPIGAKHITHDLAIGLRTRLEEAEKIKLKLSGPDNSQMSISSSEES
                     DYLDVSEFGLDEHSIPKKFLYEVIDARLNEIFKLIALEIEKAGLTARLPAGAVLTGGG
                     ALTANIEKTAKKALRMPVRIGVPRGLTGLIEEIQGPAFSASVGSVLYGLSMLNNKDGF
                     SPEPSRGGFAAPAKGFIEKLKSFLP"
     gene            2595..3737
                     /gene="ftsZ"
                     /locus_tag="PLF29_03095"
     CDS             2595..3737
                     /gene="ftsZ"
                     /locus_tag="PLF29_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HXK52947.1"
                     /translation="MLIKPEIERFAKIKVVGVGGAGGNVLNTLIESQQIVGVEYVAVN
                     TDAQDLSKNKAFVKLPIGQEITNGLGSGANPEIGRKAAEESRDLIQSNLENSDMVFIT
                     AGMGGGTGTGAAPVVASIARELGALTVAVVTKPFNFEGSQRRENAENGLVQLKREVDA
                     LIIIPNQKLLEIADEGMSVIDAFRVSDSVLSQGVQGISDLIMMPGTINVDFADVKTVM
                     KNAGSALMGIGVGTGEGRAVLAARAAISSPLLENNIDGAKGILFNVVGGSDLGMREVD
                     DAARVINTSASPDANIIFGYRVDPSLNDQVKITVIATGFEQEDELIRSRMNLDALKRK
                     DPEGLQELRPKTNKNEEVSTDKEKKQNQNIDDDSELYDIPAFLREN"
BASE COUNT         1218 a          671 c          879 g         1051 t
ORIGIN      
        1 gataaggtta gggggaagta tttcttacga aagtttgtgt ttgtcgacga gattggcgag
       61 gcctactccc gtgctgattt tgttctatgt agagcaggcg cacatactgt tgcggagctt
      121 ttagctcttc gtaagcctgc aatacttatt cccattccgt gggtctcgca taacgagcag
      181 tatgaaaatg ctcgatatgt tcaaaaattc ggtcttggag taattcttga tgagcgtaat
      241 atcagccctc cggttttact acaagagata aaaaaattct ccgaaaatat aaaatcatat
      301 aaatataacg acgagagcga ccttcacata aaattaagct ctaatgcggc cattgatatt
      361 gccggggtta ttttagaggt cgctaaggca caaaaacgaa aagacgttaa aaatgtctga
      421 tatttatctg ccaaaaaaaa tgcgaaaaaa aagagaaagc agcgctgcgc agcgtcgaaa
      481 caactctttt atatacgcga agagaaaagc atttatgctt ttcaggaggt ttactgcagt
      541 tttagtatct cttactgtaa tttgtgcttt tgctgttggt gtaaagaaaa gtattctgga
      601 tgaaaagttt ttcaatgttt cgtcctatat tgttgtaggg gcagggaact atgtaaataa
      661 agacgatgtt gagcagttgg ttcgtgccaa ttccttcgat caacttattt ttagagtcga
      721 tgtttcaggc atcgagtctc gtctcttaga aatatttaaa ggtggcagaa aatttacaat
      781 aactaaagaa tatcccgata aattaaagat cgaggtagtc gagagaaagc ccatagccat
      841 tattagcaat cccggcagtg aagatttttt tattgttgat gacgaagggt atttacttgg
      901 gcaggtcgat cgaagagcct atgatcttcc tattatttct tacaatggag agctacaagt
      961 aggatatacc cttgatgcaa aagtagttcc cgtatacctt gaattgctaa aggctctcga
     1021 ggagaaaaaa gttaaagcca caagtttaag cataacaaac gattacgttc gtttttattc
     1081 ggagaacaat gtagaagtcc ttgtttctat tgaaaaaaac attagaacct ctgtgggcat
     1141 actcacggca ttaattcgtc agttgtcgtt agggggagag tcgtttaaca aaattgattt
     1201 gagatatgat aaagtaatag taaaataatt accatatgcc taaagaaaga attattacag
     1261 gaatagacat cggatcctca aaagtgagta caattatcgc ctcactcaat aacggcaggt
     1321 tgtcagtaat cggggtttca ggatccgtgc cttcaaaagg tattactaag ggtaatgttt
     1381 cggacattga tgacacagtt gagtcaattt caaaaagcat agaacgtgcc gagaggatgg
     1441 cgggtgtttc aatatcttct gcttttataa cactaaacgg aagtcacatt aaaactcaga
     1501 attcacatgg ggtggttgct atttcaaatc caaactctga gattaatgaa tacgatatcg
     1561 cacgagtaac cgatgcggcc agagcagtaa cgctcccttc gtctgaggag ataatccatg
     1621 ttattcctag agatttcaaa gttgactctc aggaaggcgt aaaaaacccc gaaagaatgt
     1681 cgggggtacg gcttgaggta gaaactcata tcatttatgg tttggcatcg gctataaaaa
     1741 atctcaccaa atgtgttcag caggttagta tagatgttac ggctcttgta tttacggcac
     1801 tagccgcatc ggaggccacg ctcactgaca ctgaaagaga attgggaact ttgttaatag
     1861 atattggagg aggcacaacc tcagtcatag catttaatga cggaagtccg attttctctt
     1921 ctgtacttcc aataggcgca aaacacataa cccatgattt agccataggc cttagaaccc
     1981 gactggaaga ggcagaaaaa attaaactaa aacttagtgg tccggataac tctcagatgt
     2041 cgatcagttc atcggaagaa tccgactatt tggacgtttc agaatttggc cttgacgagc
     2101 attcaatacc taaaaagttt ttatatgagg ttattgacgc gagattaaac gaaattttta
     2161 aattgattgc actcgagata gaaaaagccg gcctgaccgc aagattgccg gcaggagctg
     2221 tgcttaccgg aggcggagca ttaaccgcaa acatagaaaa aacagcaaaa aaagcattac
     2281 gcatgcctgt aagaattgga gtgcctcgag gattgaccgg tcttatcgaa gagatacagg
     2341 gccctgcgtt ttctgcttcg gtagggtcag ttctgtacgg actttcaatg ctaaacaata
     2401 aagatggctt ttcacccgaa ccctcaagag ggggcttcgc tgctcctgct aaaggattca
     2461 ttgagaagct aaaatccttt ctgccctaaa aatagtatgt cattacgttg tattttgtga
     2521 aaacttgcac gagataaaat gtgcgtttcc agtataatgt aatgacaata taaaatttga
     2581 tagaataaaa aactatgttg attaaacctg aaatagagag atttgcgaaa ataaaagttg
     2641 ttggagttgg aggtgcggga ggaaacgttc taaacacctt aattgagtct cagcaaatag
     2701 tcggcgtaga gtatgtggcc gttaatacag atgctcaaga tttatcgaaa aataaagcct
     2761 ttgtgaaact tccgattgga caagaaatta caaacggttt aggttcagga gcaaaccctg
     2821 agataggaag aaaagcagca gaagaatcta gagatctaat tcaaagtaat cttgaaaatt
     2881 ctgacatggt ttttattact gcaggtatgg gcggagggac aggtaccgga gcagcacctg
     2941 ttgttgcatc tattgcaagg gagctgggcg cattaactgt tgcggttgta acaaaacctt
     3001 ttaattttga agggtctcag aggagagaga atgccgagaa cggcctggtt caattaaaga
     3061 gagaggtaga tgctttaata attattccga atcaaaaatt attagaaata gcagacgagg
     3121 gcatgtcggt aattgatgct tttagggttt ccgacagtgt gctcagtcag ggagttcaag
     3181 gaatttctga tttgatcatg atgcccggaa caataaatgt agattttgcg gatgttaaaa
     3241 cagttatgaa gaacgccggt tctgcgctaa tgggcattgg cgttggaaca ggagaggggc
     3301 gcgcggtctt agccgcgagg gcggcaatat cctctccttt attggagaac aacatcgatg
     3361 gtgctaaggg tatacttttc aacgttgtgg gaggttcaga tctgggaatg cgggaagttg
     3421 acgatgccgc tagggttatt aatacgtccg caagtccaga tgctaatatt atttttggat
     3481 acagagttga cccatctctt aatgatcaag taaaaatcac tgttattgca accggctttg
     3541 agcaggaaga tgagcttatt cgtagcagaa tgaaccttga tgcacttaag cgtaaagatc
     3601 cagaaggatt gcaggagcta aggccaaaaa caaataagaa cgaagaagtt tccacagata
     3661 aagaaaaaaa gcaaaaccag aatattgatg acgacagcga gctctacgac ataccggcat
     3721 ttcttcgaga gaactagtta gcgggtctag tagccctcct tgataaaaca ccataaaaaa
     3781 ttggcgcatt cccactttat tttataattt cgttactta
//
LOCUS       DAOSWN010000061         3537 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_18774_length_3537_cov_4.036186, whole genome shotgun sequence.
ACCESSION   DAOSWN010000061 DAOSWN010000000
VERSION     DAOSWN010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18774_length_3537_cov_4.036186"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PLF29_03100"
     CDS             complement(<1..364)
                     /locus_tag="PLF29_03100"
                     /inference="COORDINATES: protein motif:HMM:NF024635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HXK52948.1"
                     /translation="MKVLLLGPQGSGKGTVGDIVSKRLGIPAISSGQLLREIPPSHPR
                     FEEINDLMGKGVLVSQDLVASLIEERVSREDCAKGYILDGWGRAMIDLKYFDPGYDKV
                     IVLVLSREECVRRITGRRI"
     gene            complement(365..634)
                     /locus_tag="PLF29_03105"
     CDS             complement(365..634)
                     /locus_tag="PLF29_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52949.1"
                     /translation="MIDKLKAVNKARKLQSDIKSQLEQIFHQEEKGNNSVLVRGDKRI
                     EKIVIDGEERSDIKAMLNDALKQIDKKSEKKMKDQAGDLMALLGL"
     gene            complement(648..1904)
                     /gene="secY"
                     /locus_tag="PLF29_03110"
     CDS             complement(648..1904)
                     /gene="secY"
                     /locus_tag="PLF29_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HXK52950.1"
                     /translation="MFTILKNVEVRRKILFTLFVVLVFRLLTHVPVPGVDATAIRNYV
                     AGSTIFGFFDLFTGGGFQNFSIVTLALGPYINASIILQLLTLMIPSLEELSKEGQYGR
                     DKIDQYTRWLTLPISILQAYGIYFLFQKQGLIQSLGVLDLSVLVFTLTAGVLFLVWLG
                     DLVTEYGVGNGVSLLIFVGIISRLPASLLQAYIQRSTYDLPTILAFVLLSLLVIIGVV
                     LVNEGTRNVALEYGRRGSKSQKVTNYLPIKINQAGVIPIIFAVSIVLIPSFLSSPLMS
                     SGNDRLVSLGVFLFNNFTSTSFLYNALYFLLVVAFTFFYTFLQFNPEKVADDIKKRGG
                     FIPGIRPGKSTKDHLTMIVVRLTLVGSVFLGLIAILPYLLQRLFGVSNLTIGGTGLLI
                     VVSVILETVRQIDSMKATRSYEGYLR"
     gene            complement(1909..2352)
                     /gene="rplO"
                     /locus_tag="PLF29_03115"
     CDS             complement(1909..2352)
                     /gene="rplO"
                     /locus_tag="PLF29_03115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HXK52951.1"
                     /translation="MELHNLTKLKGTKRKAKRLGRGYGSGVGGHVVGFGQKGQKARSG
                     NSIPFGFEGGQVPLYKKMPEIGGFRNPTAKSIVGISVSRLNKFKDGSVVTPTDFVKAG
                     ILKKLPKHGVKLLNTGKIEVKVTVYGFLLSESANTALTKAGCTIK"
     gene            complement(2352..2798)
                     /gene="rpsE"
                     /locus_tag="PLF29_03120"
     CDS             complement(2352..2798)
                     /gene="rpsE"
                     /locus_tag="PLF29_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002345056.1"
                     /note="located at the back of the 30S subunit body where
                     it stabilizes the conformation of the head with respect to
                     the body; contacts S4 and S8; with S4 and S12 plays a role
                     in translational accuracy; mutations in this gene result
                     in spectinomycin resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HXK52952.1"
                     /translation="MPQYDASQFEERIVETKRVSKKNKGGNKPGFTALVVVGNKSGTV
                     GAALGKAPDISSAIRKASKQARKDVVEININNNTIAHSVVMKYKASIVKMMPAPEGSG
                     IIAGGAVRHVMELAGIKDVSAKLLGSRNKITNVFCTIKALETIKGR"
     gene            complement(2800..3159)
                     /gene="rplR"
                     /locus_tag="PLF29_03125"
     CDS             complement(2800..3159)
                     /gene="rplR"
                     /locus_tag="PLF29_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011668483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HXK52953.1"
                     /translation="MQRTSDIANSKIKRKLRARKNIVGTAEKPRLSVYRSNKYTYCQA
                     IDDTTGKTLATAFNLVKETHVGKNKVAAAFDLGEKFAKVALEKKITKVVFDRNGYRYH
                     GRVASLAEGARKGGLEF"
     gene            complement(3159..>3537)
                     /gene="rplF"
                     /locus_tag="PLF29_03130"
     CDS             complement(3159..>3537)
                     /gene="rplF"
                     /locus_tag="PLF29_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HXK52954.1"
                     /translation="KETKNTPAFWGLTRALIANMVQGVTEGYTKDLELVGVGYRVKSE
                     NPQKISLSVGYSHPVEVEAPEGITFAVDGNQNITIHGIDKQLVGLTAAKIRKIRKPEP
                     YKGKGIKYKGEVVRRKAGKAGKK"
BASE COUNT         1006 a          792 c          569 g         1170 t
ORIGIN      
        1 agatccttct tcctgtaatt cttctcacac actcttctct ggataatacg agaactatca
       61 ctttgtcgta gccgggatca aaatacttta aatcaatcat cgcacgtccc caaccatcca
      121 aaatataacc tttagcacaa tcttcccttg acactctctc ttctatcaaa gatgccacta
      181 ggtcttggga taccaacaca cccttaccca taagatcgtt tatctcttca aaccttgggt
      241 gtgatggagg gatttccctt agtagctgcc ccgaagatat tgccggaatt ccaaggcgtt
      301 tagacactat atcccctact gtacctttac ctgagccttg ggggcctagc aacaaaactt
      361 tcatttataa tcctaataac gccatcagat cgcctgcctg atctttcatt tttttctccg
      421 attttttatc aatctgcttc aaagcatcgt ttaacatggc cttgatatca gatctttcct
      481 caccatctat gactattttt tcaatgcgct tatctcctct tactagtaca gagttgttgc
      541 ctttttcttc ctgatgaaaa atttgctcta attgagattt tatatctgac tgtaattttc
      601 tggctttatt tactgctttc aatttatcta tcattagaaa tgcctttcta ccttaaatac
      661 ccttcataac ttcttgtagc tttcattgaa tcaatttggc gtaccgtttc gagaataaca
      721 gaaactacaa tcaataaacc ggtaccgcca atagttaagt ttgatacgcc aaacagtctc
      781 tgaagcagat acggcaagat agctattaaa ccaagaaaaa ctgatcctac taaggttaac
      841 cttacaacaa tcatggttaa atggtctttg gtcgatttgc caggtctaat tccaggtata
      901 aagccgcctc tcttttttat atcatctgca accttttcag ggttaaattg caaaaatgta
      961 tagaaaaaag taaaagcaac aactaacaga aagtaaagtg cattatataa aaaggatgtt
     1021 gacgtaaagt tattaaataa aaaaactcct aaagaaacca gcctgtcgtt tccggaagac
     1081 ataagaggac tgctcaaaaa agaggggatt aaaacaattg aaactgcaaa aataataggt
     1141 ataactccgg cctggttaat ttttatcggc aagtaattgg taactttctg ggatttagag
     1201 cccctgcggc catattccag agctacattt cttgtacctt cgttgaccaa aacgacaccg
     1261 ataattacaa gtaaggacag gagtacgaaa gctaatattg taggaaggtc gtaagtagaa
     1321 cgctgtatgt atgcctgcag caatgatgcc ggcaacctac taataatacc tacaaaaatt
     1381 agtaaagaga caccgtttcc tacaccatac tcagtaacta aatctcctaa ccatactaag
     1441 aaaagtaccc ccgctgtaag tgtgaatact aaaacagaga gatccaacac accaaggctt
     1501 tgaatcaatc cttgtttttg gaacaaaaaa taaattccat atgcttgaag tatcgatata
     1561 ggtagcgtta gccagcgcgt atactgatca atcttatctc tgccatattg gccctccttc
     1621 gagagctctt cgaggctcgg aatcatcaag gtgagaagct ggagtatgat agaagcattg
     1681 atataaggac ctaaagctaa agttactata gaaaagtttt gaaaaccccc acccgtaaat
     1741 aaatcaaaaa atccaaaaat ggtgctacct gcaacatagt ttcttattgc tgtcgcgtct
     1801 acgcctggaa ccgggacatg agttagtaaa cgaaaaacta aaactacaaa aagtgtaaaa
     1861 agtatttttc ttcttacttc gacgtttttt aaaattgtga acatgaattt attttatagt
     1921 acagcctgct tttgttaaag cagtattagc tgattccgaa agtaaaaacc cataaactgt
     1981 cactttaact tctatctttc ctgtgttaag aagtttgaca ccgtgctttg ggagtttctt
     2041 taaaatgcct gctttaacaa aatcggttgg ggtcactaca gaaccatctt taaacttatt
     2101 tagcctgctt actgaaatcc ctacaataga ttttgctgta gggtttctaa atccacctat
     2161 ttccggcatc tttttatata aaggtacctg accgccttca aatccaaaag gaatgctgtt
     2221 tcccgacctg gctttctggc ctttctggcc aaaacctaca acatgcccgc ctactccaga
     2281 tccgtatcct ctcccgagtc ttttagcttt tcttttcgtg ccttttaatt ttgttaagtt
     2341 atgtaactcc attatcttcc ttttatagtt tcgagcgcct taatggtgca aaatacatta
     2401 gtaattttat ttcttgatcc taaaagcttt gccgaaacgt ccttgatacc cgctaactcc
     2461 ataacgtgtc taacagctcc gccggcaatg attccagaac cttcaggagc aggcatcatt
     2521 tttactatag aggccttgta tttcataaca accgaatgtg caattgtatt gttattaata
     2581 tttatctcta caacatcttt tctcgcctgc tttgatgctt ttcttatagc tgaagaaata
     2641 tcgggcgcct tccctaaagc cgccccaact gtgccgcttt tattcccgac aacaaccagt
     2701 gcggtaaaac ccggtttatt tccaccttta ttcttttttg aaactctctt agtttcaact
     2761 attcgttctt cgaactgtga tgcatcatac tgtggcatat taaaattcca atcctccctt
     2821 tcgggctcct tctgctaaag aggctactct accgtggtat ctatagccgt ttcgatcaaa
     2881 tactactttt gtgatctttt tttctagtgc tactttagca aacttttcgc ctaaatcaaa
     2941 ggctgcagcc actttatttt taccaacatg tgtttctttt accaaattaa aagcggttgc
     3001 caatgttttg cctgtagtgt cgtctattgc ctggcagtaa gtatatttat ttgatctata
     3061 tacagagagt cttggctttt cagctgtacc aacaatattt ttccttgctc ttagttttct
     3121 ttttattttg ctattagcaa tatcagatgt tctttgcatt attttttacc tgcctttccg
     3181 gcttttcttc taactacctc gcctttgtat tttataccct tacctttgta aggctccggt
     3241 tttctaattt ttctaatctt tgctgcagtt aggccaacta attgcttatc gattccgtga
     3301 attgttatat tctgattacc atctactgca aaggttatgc cttccggagc ttcaacctct
     3361 accggatgcg aatagcctac cgagagagat attttttgag gattctcgga cttaactcta
     3421 tatcctacac caactagctc gaggtctttg gtgtaacctt ctgtaactcc ttgaaccata
     3481 ttggcaatta gagccctcgt taaaccccaa aatgcgggag tattttttgt ttccttt
//
LOCUS       DAOSWN010000062         3456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_19423_length_3456_cov_5.104087, whole genome shotgun sequence.
ACCESSION   DAOSWN010000062 DAOSWN010000000
VERSION     DAOSWN010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19423_length_3456_cov_5.104087"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1294
                     /locus_tag="PLF29_03135"
     CDS             <1..1294
                     /locus_tag="PLF29_03135"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXK52955.1"
                     /translation="NLNYTRYRPVYINNTNLWNIRMDKPYNEVYNLMATLGIAGVIAF
                     IFIAQRTIKAGTESILAANENTEKLVATLMIAMAITYVFTYANILNSFMLMLFTALTT
                     IMAVKNSGVITQIIELKIAAIQKAPKNVGTNTGLLKSFHVFAAIPVLAVALLGSILFY
                     RAYAAEYYFAGSIKAFAQGDGVKMYLYQQKAISYAPTRAEYRNAYAQTNMLIAQNMSA
                     QEEPTEEESSIIQNAITEAVNQVKTSTEVLDPLNANSWELRATIFKQLLSVAKDADTL
                     TIQAYENAIRLDPNSPRLRLDLGNVYFSQEKYLEAANLFRQAANLKGDYANAHYNLAQ
                     ALEKIEKYDLAKTEYEITKRLVQPDSEDYNMVVAAIEALPEPQVAGTSDEKPTVEELT
                     TQEPGPNNETQVTLQEPLKDPVLDPSIELDKVPTTETN"
     misc_feature    1367..1485
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            1569..2099
                     /gene="rplJ"
                     /locus_tag="PLF29_03140"
     CDS             1569..2099
                     /gene="rplJ"
                     /locus_tag="PLF29_03140"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HXK52956.1"
                     /translation="MPNNKNKQAVKDLSEKVAKAKSIVFVNYHGIKATEMSDLRSKLR
                     AVDASSSVAKNTLLKIALKENNLTDSNLDTELKDGTMTIFGFADAISSIKTLFGFIKT
                     QENSLPVVKSGFVEGKFLTANELTELNSLPSREELLSKLIWGLKSPLQGFAGATSGVQ
                     RKFIYAVSQLASKKEA"
     gene            2192..2593
                     /gene="rplL"
                     /locus_tag="PLF29_03145"
     CDS             2192..2593
                     /gene="rplL"
                     /locus_tag="PLF29_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HXK52957.1"
                     /translation="MSEVTLSKNAEKVLEMVEQMTIVELNELVKAMEEKFGITAAAPM
                     VMGSMPAAGAVAEAPGDEQTEFDVIIADAGSNKIAVIKAVREVNQSLGLVEAKELVES
                     APKALLEGAKKEDAEAAKKKLEEAGAKVELK"
     gene            complement(2862..>3456)
                     /locus_tag="PLF29_03150"
     CDS             complement(2862..>3456)
                     /locus_tag="PLF29_03150"
                     /inference="COORDINATES: protein motif:HMM:NF014764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52958.1"
                     /translation="NRLGIYNAEEKEVIIFNNRGGKAGASLMHPHSQVVASKGFPGTI
                     EQEKSSALHYYNSHVMCYWCHEMEKEIVNKQRVVLESKHFVVYVPEACRWSYEMRLLP
                     KKHTPTFAYTTEPELYDLADVLQKVLKAYNHLFDRPDRNFWIHTERYEPYHWHIGFIP
                     HIKVFGALELGAGIWVSDKATSEDAAAQLRDVIATFK"
BASE COUNT         1190 a          710 c          689 g          867 t
ORIGIN      
        1 caacttaaat tacacaagat acaggcccgt ttacataaac aacaccaacc tttggaatat
       61 caggatggat aagccttaca atgaagtgta taaccttatg gcgacactag gtatagccgg
      121 ggtgatagcc tttatattta tagcccaaag aactattaag gcagggacag agtcgatttt
      181 agccgcaaat gagaatacgg agaagcttgt tgcaacttta atgattgcca tggccattac
      241 gtatgttttt acttacgcta atattttaaa tagcttcatg ctaatgcttt ttaccgccct
      301 tacgactata atggcagtaa aaaatagcgg agtgattacc caaattattg aactcaagat
      361 agcggcaatc caaaaagccc ctaagaatgt gggcaccaac acagggctac taaagagctt
      421 ccacgtgttt gctgctatac cggtgctagc cgtagccttg ctgggatcaa ttctatttta
      481 cagggcctat gctgcagaat attacttcgc aggttctata aaggcctttg cgcaaggaga
      541 tggtgtaaaa atgtacctat atcagcagaa ggccatttcc tatgctccta caagagcaga
      601 atatcgaaat gcttatgcac aaaccaacat gttgatagct caaaacatgt ccgcgcagga
      661 agagccgacc gaggaagaaa gctccatcat tcagaatgcg attaccgaag ccgtaaacca
      721 ggtaaaaacc agcaccgaag tcttagaccc tcttaacgca aattcttggg aactaagagc
      781 aacaatcttt aaacaactgc ttagtgtggc taaagatgcc gatactttga caattcaggc
      841 ttatgaaaat gccatcaggc tggatccaaa cagcccgaga ctaaggctgg atttaggcaa
      901 tgtctacttt tctcaagaaa aatatcttga agcagcaaat ctttttagac aagcggctaa
      961 cttaaaagga gattacgcta atgcgcacta taacttagcg caagccctag aaaaaattga
     1021 aaaatacgac ctagcaaaaa cagagtacga aattaccaag agactggtgc agcccgattc
     1081 cgaagactac aatatggttg ttgccgctat agaagcgctc ccagaaccac aagttgcagg
     1141 aacatcagat gagaagccaa cggttgaaga gctaacaacc caagagccgg ggccaaacaa
     1201 cgaaacacaa gttactcttc aagagccttt gaaggatcct gtattagatc cctccataga
     1261 gcttgataaa gttcctacga ccgagactaa ctaaacacaa gcaacaacta taaattgcct
     1321 gaatgttggt ctcactgtgc gcgatacttg acactaagaa aaaaaattgt aaactaatgt
     1381 agtacctaag acagcaaggg ccttaaagct taataatctt gccgaggcaa ataaaatttt
     1441 ataacaatat ttttatcacc ctcgtgtagc attatttata ggctaaggat caaccagaaa
     1501 ttaattgaga tttaaaagtc tcttgcagtt gcagattaat tgactaataa agtttgaaag
     1561 aaagaattat gccaaataac aaaaacaaac aagcagtaaa agacctgagt gaaaaagtag
     1621 ctaaagcaaa atcgatagtc ttcgtaaact atcatggaat aaaagctact gaaatgagcg
     1681 acttacgatc aaagcttagg gctgtagacg catcttcttc tgttgcaaaa aacacgttgt
     1741 tgaagatcgc actcaaagaa aacaatctaa ctgactctaa tctagacaca gaacttaaag
     1801 acggtaccat gacaatattc ggcttcgcag atgccatttc aagcattaaa accttattcg
     1861 gctttataaa aactcaagaa aattctcttc cggtagtaaa atcggggttt gttgaaggca
     1921 agtttttaac cgccaacgaa ctaacagaac taaactcatt acctagcaga gaagagcttt
     1981 taagcaagct gatttgggga ttaaaatccc ctcttcaagg ctttgccgga gcaacttctg
     2041 gagtacaaag aaaatttatt tatgcagttt cacaacttgc ttctaagaaa gaggcttgat
     2101 tgcaggttct tggaattatc caaaaaccct atgtgctaag gcacgtaatt aagaatatag
     2161 aaattgtaat ttattaagga ggtgtagatc tatgtcagaa gtaacactaa gtaaaaacgc
     2221 agaaaaagta ctagaaatgg tagaacaaat gaccattgta gagcttaacg aactcgtaaa
     2281 agctatggaa gaaaagtttg gaattactgc agcggcacct atggtcatgg gctcaatgcc
     2341 tgcggcggga gctgtagctg aagcaccagg agatgaacaa acagaatttg atgtcataat
     2401 tgcagacgca ggttcaaata aaattgctgt aattaaagca gttcgagaag tcaaccaatc
     2461 attgggatta gtagaagcaa aggaattagt agagtcagct cctaaagcat tgttggaagg
     2521 ggcaaagaaa gaagacgctg aagccgctaa gaagaagctg gaagaagcag gagcaaaggt
     2581 tgaacttaaa taaactagaa ctagaacttt aggaatgttt ccgaggcacc ccgcaaagcg
     2641 gggtgccttc attatccaaa ccttttaaaa ctatccaata aaccaccact agctgacgca
     2701 ggaggtttgc tgtaacttct ttacgctgca aagcccaaca tgttgggtaa actacaccta
     2761 ccagcttacg cgagtagttt taagtaacta aattataaaa acacaaatac caaaccgtga
     2821 gaaaaaagtg gctgaggaaa aatccaaacg cccgtagtcc cctacttaaa agtagctata
     2881 acatctctaa gttgcgcggc agcgtcctct gaggtggcct tatcactcac ccatattccg
     2941 gcgcctaact caagcgcacc aaaaacttta atatgaggga taaacccaat gtgccaatgg
     3001 taaggttcat acctttcagt gtgtatccaa aaatttctat caggcctatc aaaaagatga
     3061 ttataagctt taagaacctt ttgcaacaca tcggcaagat catatagttc gggttcagta
     3121 gtataagcaa aagtcggtgt atgctttttc ggcaacaagc gcatttcgta actccatcgg
     3181 cacgcctcgg gcacataaac tacaaaatgc ttggactcta aaactacgcg ctgtttgttt
     3241 actatttctt tttccatttc atggcaccaa taacacatca catgcgaatt gtagtagtga
     3301 agggcactgc tcttctcctg ctcaatagtt ccaggaaatc ctttggaagc tactacctga
     3361 gaatgaggat gcattaggga ggctcctgcc tttcctccgc gattattaaa tataattact
     3421 tctttttctt cagcgttgta aatgccaagc ctattt
//
LOCUS       DAOSWN010000063         3372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_20224_length_3372_cov_5.833886, whole genome shotgun sequence.
ACCESSION   DAOSWN010000063 DAOSWN010000000
VERSION     DAOSWN010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20224_length_3372_cov_5.833886"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /gene="rho"
                     /locus_tag="PLF29_03155"
     CDS             <1..741
                     /gene="rho"
                     /locus_tag="PLF29_03155"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HXK52959.1"
                     /translation="MLVAPPKAGKTWLLQDIANGITKNHPEVVLMVALIGERPEEVTD
                     MRRNVNGEVYASNFDQDPADQTRVAEICLEKAKRTAEKGKDVVILMDSLTRLARAYNM
                     VVQPSGRTLTGGFDPSALYPAKRFFGAARNLEEGGSLTIIATALVDTGSRMDDVIFEE
                     FKGTGNMELKLDRALSERRIFPAIDIKSSSTRREDLLYDKNSMEMVHKLRRMIDLLDD
                     KETTDLVLQQLKKSKSNEDFLKTLGQPA"
     gene            899..2206
                     /locus_tag="PLF29_03160"
     CDS             899..2206
                     /locus_tag="PLF29_03160"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXK52960.1"
                     /translation="MLNCAFFDKIGTQKVMPFLKRKQKNLGSKIKSTRLWTVAINRPK
                     SRRFSQLSFEGFVPKGTNKLNTVFRLTFSFFSYIFKYFKLKTTKTLKIPGKVALFSVT
                     TFMSLKAFMVRKLIWSQGKLGRPIATGVVMSVVLALFFLGKILNSSTLVVSQELDSGY
                     LSSTTDIIPQTNTALTTVPESRKRNESFIYIVEPGDTLSSIGNKFKISTDALKYVNSL
                     TDSSIIRTGQEIIIPPVSGLIHTVKSGDTLNSIARKYDVAPQAIADFNYILDTSKLAL
                     GTELVIPGAKVPEPVIPAAPSGPAAPPGNFVSAPSTGKWCVWPTGPRIITQYYSWYHN
                     GIDIATPSGSAMPALFACAGGVVTRAGWDPFGLGLHVRIDHGNGFETVYGHMSSISVS
                     YGQRVEQGQMIGVMGSTGRSTGPHVHFTVNYNGASQDPLKYTY"
     gene            2464..>3372
                     /gene="obgE"
                     /locus_tag="PLF29_03165"
     CDS             2464..>3372
                     /gene="obgE"
                     /locus_tag="PLF29_03165"
                     /inference="COORDINATES: protein motif:HMM:NF008956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HXK52961.1"
                     /translation="MIDTAVIKVKAGKGGDGKVSFRREKYIPKGGPDGGDGGDGGNVY
                     LITDHNMATLLDFKSKPTYEGQNGEAGGSVQMSGAKGEDLYIKVPEGTLVYEILPDRK
                     ILLVDMVAQEDESQKVFLLARGGKGGKGNTRFKSSTNRTPIQFTKGTLGDEKDIQLEV
                     KLVADIGLVGKPNAGKSTLINKLTNANAKTASYPFTTLTPNLGTALLKSGQNVIIADI
                     PGLIEGASSGKGLGDDFLRHIERTRVICHVIDPLDGVVFDPDSYDDGKDFSKIVASNA
                     VGCYKTIRQELSEYESDLTSKPELVVV"
BASE COUNT         1014 a          620 c          777 g          961 t
ORIGIN      
        1 atgcttgttg cgcctccaaa agcgggtaag acctggttat tacaagatat agcgaatggt
       61 attacaaaaa accatccgga agtagtatta atggtagctc ttattggcga acgccctgag
      121 gaagttaccg atatgagaag aaacgtaaat ggggaagtat acgccagtaa ttttgatcag
      181 gatcctgctg atcaaactcg tgtagcagag atttgcctag aaaaggcgaa aagaaccgcg
      241 gaaaaaggaa aagatgttgt aatacttatg gattctttaa ccaggcttgc tagagcttac
      301 aatatggtag ttcagccttc tggccgcaca ctaaccggtg gttttgatcc ttctgctctg
      361 tatcctgcaa aaagattttt cggcgctgcc agaaatttag aagagggcgg tagtttaaca
      421 attattgcta ctgcgcttgt tgataccggt tctagaatgg atgacgtaat ttttgaagag
      481 tttaaaggga ccggaaatat ggagttaaag ttggatagag ctttgtccga gagaagaatt
      541 tttccggcga ttgatataaa atcaagcagt acaaggcggg aagatttgtt atacgataaa
      601 aattcaatgg agatggtgca taagttaaga agaatgatag atttgcttga tgacaaagaa
      661 actaccgacc ttgttctaca gcagctcaaa aagtctaaat ccaacgagga tttcttgaag
      721 acgctaggcc agccggccta attaatctgc ttaacgtgcg gctttacaat ctcgctactt
      781 aaaattcctg cataagttag tagatgtagt ttacccaaca tgttaggctt tgcaacgcaa
      841 agagattgca gcaaaccacc cagcgcaagc tggtggtggt tgataatgtc aagctaaggt
      901 gcttaactgt gctttttttg ataaaatagg cacacaaaaa gtcatgccgt ttttaaaacg
      961 aaaacaaaaa aaccttggct ctaaaattaa gagcaccaga ttatggacag tcgctattaa
     1021 taggccgaaa tctcggaggt tttctcaatt gagttttgaa ggttttgtac ctaaaggtac
     1081 aaataaactt aatactgttt ttcgtttaac tttttccttt ttctcttata ttttcaagta
     1141 cttcaaactc aagacaacaa aaacattaaa aatacccggc aaagtcgccc tattttcagt
     1201 cactactttt atgagtctta aagcttttat ggtacgtaaa ctaatatggt ctcagggtaa
     1261 gcttggtagg cctattgcaa caggtgtagt tatgtctgtg gtgctagccc tgttttttct
     1321 tggaaaaata ttaaatagct ctactttggt tgtgagccag gaactggatt ccggatatct
     1381 atcaagtaca accgatatca taccccaaac aaatacagct cttacaacag tgccggaaag
     1441 tcgtaaaagg aatgagtctt ttatttatat cgttgagccg ggggatactt tatcttcaat
     1501 cggtaataaa tttaaaatct caaccgatgc tttaaaatat gtaaacagcc ttaccgactc
     1561 ttccataatt cgtacaggtc aggaaattat cattcctccg gtttcgggat taattcacac
     1621 cgttaaatcg ggcgacactc taaacagcat tgcccgtaag tacgatgttg ctcctcaggc
     1681 tattgcagat tttaactata ttctagatac gtctaaatta gctttaggta cagagctcgt
     1741 gattcccggc gccaaggttc ccgagccggt tattccggca gctccttcgg gccctgcggc
     1801 tcctccggga aattttgtgt ccgcgccttc tacaggaaaa tggtgtgtgt ggccgaccgg
     1861 gccaaggatt attacccaat actattcttg gtatcataat ggaatcgata tagctactcc
     1921 ttcaggctcg gccatgcctg ctctttttgc ttgcgcgggc ggtgtagtaa caagagccgg
     1981 ttgggatccc ttcggcctag gactgcatgt aaggatagat catggtaatg gttttgagac
     2041 tgtgtacggg catatgagca gcatttccgt aagttatggt caaagagtag agcaggggca
     2101 aatgattgga gtgatgggta gcacagggcg ttctaccgga cctcacgtgc attttactgt
     2161 taactacaac ggcgcgtcgc aagatccttt aaaatatact tattagttta taattttgtt
     2221 actaaaacca cttgcgtaag ctgtagatgt agtttaccca acatgttgac tttgcaacgc
     2281 aaagaaacta ttgcaaacca cctgctgcgt aagctggtgg ttgattaatt gtgctgatgc
     2341 agagagcttc atttctcaga cgagggcgta aaagcgtaga gtaaagaaga tagtacaaaa
     2401 ctagattcgt agtatttcat tcctctaggc ttaaagcaat ttgagtgcta gaattgccta
     2461 actatgattg atacagctgt aataaaggtt aaagccggta aaggcggaga tggaaaagtc
     2521 tcttttagaa gagagaaata tatcccgaag ggtggccccg atggtggtga tggcggtgac
     2581 ggggggaatg tttatttaat aaccgatcac aacatggcta cattgctcga ctttaaatct
     2641 aaaccaactt acgaaggtca aaatggtgag gccggaggaa gtgttcagat gtcgggtgca
     2701 aagggtgaag atttatatat taaagttccc gagggtacgt tagtgtacga aatattgcca
     2761 gatagaaaaa ttttactggt tgatatggtt gcccaagagg atgagtccca aaaggttttt
     2821 ttacttgcac gcgggggcaa gggcggcaaa ggaaacactc gctttaaaag ctctactaat
     2881 agaaccccta ttcagtttac taaggggact ttgggcgacg agaaagatat tcagttagaa
     2941 gttaaacttg ttgcagatat tggtttagtt ggtaaaccaa atgcaggaaa aagcacactt
     3001 attaataaac tgacaaatgc aaatgcaaaa accgcgagct atccttttac taccctaact
     3061 ccaaatttag gcacagccct gctaaaaagt ggtcaaaatg tgataatagc tgatataccg
     3121 ggattaatag aaggggcctc ttcgggcaag gggctggggg atgacttttt aagacacatt
     3181 gaacgtacta gagtcatttg tcatgtaatt gatccgctgg atggcgttgt atttgatccc
     3241 gattcttatg atgatgggaa agatttttct aagattgttg cttctaacgc agtaggttgt
     3301 tataagacta tacgacagga actgtcggaa tatgaaagcg atctaacaag taagcctgaa
     3361 ctagttgttg tg
//
LOCUS       DAOSWN010000064         3067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_23446_length_3067_cov_4.540837, whole genome shotgun sequence.
ACCESSION   DAOSWN010000064 DAOSWN010000000
VERSION     DAOSWN010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23446_length_3067_cov_4.540837"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /gene="serS"
                     /locus_tag="PLF29_03170"
     CDS             <1..896
                     /gene="serS"
                     /locus_tag="PLF29_03170"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HXK52962.1"
                     /translation="KDHVELGESLGIISFEDGAKVSGSQFYFLYGAGVLMELALVQYA
                     FEKLSKAGFTPTLTPDLAKSRYYLGTGYAPRGDEAQTYTIEGEDLGLIATAEVTLAGK
                     HADEVLLLENLPLKYVGYSHCFRKEAGAYGKYSKGLYRVHQFTKVEMFIYCKPEESMQ
                     MHDYLLEMEESIYQGLGISYRVLKQCTGDLGAMAARKYDIEAWMPGRKDYGEVTSTSN
                     CTDYQARNLNIKYKLDTGGTEYVHMLNGTAVATSRTIIAILENYQQADGSVLIPEVLK
                     PYMNGLEVIRPISLRDKPRPQ"
     gene            947..1642
                     /locus_tag="PLF29_03175"
     CDS             947..1642
                     /locus_tag="PLF29_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52963.1"
                     /translation="MYSVFSNIKEPEVILSTSSDFSKSLKVVKVGTTKKLINNGVIHS
                     VAHDSKSAEALVWGNMVQVIKEQEPELQSVLVMGLGGATFQHLLAKAYPGVHIVSVEI
                     DPVVVDIARTHFRADEIPNHSIIVADACAVVVSPEKYGLRKQSFSAVVVDIFIGDVYP
                     DLGNSGNFFSLLKSMTHPGGLVVFHRYYKTDDHQDEAHMFLDNVKDFFTNVETEIIPG
                     KSNADNILIFGRA"
     gene            1645..2466
                     /gene="uppP"
                     /locus_tag="PLF29_03180"
     CDS             1645..2466
                     /gene="uppP"
                     /locus_tag="PLF29_03180"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HXK52964.1"
                     /translation="MTIFQALVLGTIQGLSEFLPISSSGHLILLPYLLRWNEQSLAFD
                     LVLHVGTSVALIVYFFRDLLDIAKALFFNIAQKRNKLQEYSRSGKYGLFALVAVIPAG
                     IAGIVLDATLEQVFRSALWVELFLLLGTILMYVAEKRFARIETKSELTLKKSIAIGLF
                     EMLALLPGFSRSGSTISGGMIVGLSRKEAARFSFIMAIPTILGAALYKIYSSFESLQQ
                     LGGLLVVVGFLSSFITGILAISFLMKFLRNNSLTTFIVYRILIVLVLLGLFYVRI"
     gene            complement(2437..2940)
                     /locus_tag="PLF29_03185"
     CDS             complement(2437..2940)
                     /locus_tag="PLF29_03185"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HXK52965.1"
                     /translation="MGYILDLDTVVYISQDLRRRKKKIIYTHGSFDLFHSGHSYLFNK
                     AKKLGDVLIVGVDSDEKVRKFKGERRPVNQHSLRAEMVNNHKDVDIVFSIEGLAENEE
                     DYYVGLYKKLSPHIVVVGNSFGFRNNFRKKLANTEFVLIDLLPEHQISTTDIIQKVLD
                     THVEKSE"
BASE COUNT          865 a          548 c          639 g         1015 t
ORIGIN      
        1 caaaagatca cgttgaattg ggagaatccc tcggaattat aagttttgaa gacggtgcca
       61 aagtttcagg ttctcaattt tattttctat atggtgccgg tgtcttaatg gagcttgcgc
      121 ttgtgcaata cgcgtttgaa aagcttagta aagctggttt tactccgacc ttgacgcctg
      181 atttagcaaa gtcccgttat tatcttggaa caggctatgc ccctagagga gatgaggccc
      241 aaacttatac aatcgaaggt gaggatttgg gtttaattgc aactgctgaa gtaactcttg
      301 ccggtaagca tgctgacgag gttctacttc tggaaaattt acccttaaaa tatgttggct
      361 attctcactg ttttagaaaa gaggccggcg cttatggaaa gtattcaaaa gggctatata
      421 gagttcacca atttacaaaa gtggaaatgt ttatttattg taagcctgaa gaatcaatgc
      481 agatgcatga ttatctgctg gagatggaag aatcaattta ccaaggcctg ggcatttctt
      541 atagggttct aaagcaatgc accggagact taggtgcgat ggcagcaaga aaatatgata
      601 tagaggcttg gatgcctggt agaaaagact atggggaagt gacatccact tcaaattgta
      661 cagattacca agcgagaaat ttaaatatta agtataaatt agacacaggc ggcactgagt
      721 atgttcacat gttaaacgga actgcagttg caacgtcaag aacaattatc gctatattgg
      781 aaaattacca gcaagcagac ggctctgttt taataccgga agttttaaag ccctacatga
      841 atggcttgga ggttattaga ccaatttctt tgagagataa gcccagacct cagtgagcat
      901 ggagtacagt atatgtttag attaaatttt attgtattat caaaatatgt attccgtatt
      961 tagtaatatc aaggagcctg aggttatatt atctacatca tcggatttca gtaaatcttt
     1021 gaaggtcgta aaagttggaa ctacaaaaaa acttattaat aatggtgtga ttcactctgt
     1081 agcgcatgat tccaaatctg ctgaggcatt ggtgtgggga aatatggtac aggtcattaa
     1141 agagcaagag cccgaactgc agagtgttct tgtaatgggt ctgggaggcg cgactttcca
     1201 gcatctcctt gcaaaagcgt atcccggtgt acatattgtt agtgtggaaa ttgacccggt
     1261 tgtagttgat atagcaagga cacatttccg tgcggacgag attccaaacc actcgattat
     1321 agttgccgat gcttgtgcgg tggttgtatc gcctgaaaag tacggactgc gtaaacaatc
     1381 ttttagtgct gttgttgttg atatttttat tggtgatgta tacccagatt tagggaattc
     1441 cggaaacttt ttttcacttt taaaaagcat gactcaccct gggggcttag ttgtgtttca
     1501 cagatactat aaaaccgatg accaccagga tgaggcccat atgtttttgg ataatgtaaa
     1561 agattttttt acaaacgttg agactgaaat cattcccggt aaaagcaatg ccgataatat
     1621 tttaattttt ggccgagctt agttatgaca atttttcaag cactggtttt aggaactatt
     1681 cagggtctta gcgagtttct acctatttcg tcctcaggac atctcattct tttaccctat
     1741 cttctacgct ggaatgagca gtctttggca tttgatttgg tcctgcatgt gggaacctcg
     1801 gttgctctta tagtgtattt ttttcgcgat cttttggata ttgccaaggc tttatttttc
     1861 aacattgctc aaaaaagaaa caagttgcaa gaatattccc gatctggaaa gtacggctta
     1921 tttgctctgg ttgcagttat tcctgcaggt atcgccggta ttgtattaga tgctactctc
     1981 gaacaagttt ttagaagtgc tttgtgggtc gagctctttt tgcttttagg cacgatttta
     2041 atgtacgtgg ctgaaaaaag atttgctcgt atcgaaacta agtcagaact taccttaaaa
     2101 aaatcaatcg caataggact ttttgagatg ctcgccttgt tgccaggttt ctccagatca
     2161 ggatccacaa tctccggagg catgatcgtt ggtttaagca gaaaagaagc ggcaagattc
     2221 tcttttatta tggctattcc cactatactc ggcgccgctt tgtataaaat ctattcctct
     2281 tttgaaagtc ttcagcaatt gggcggcctg cttgtagttg ttggttttct atcgagcttt
     2341 ataaccggaa ttttagcaat aagcttttta atgaaatttt tgcgtaacaa ttctttaaca
     2401 acctttatag tctataggat tcttattgtt ttggttctac tcggactttt ctacgtgcgt
     2461 atctaatact ttttgaatta tgtcggtggt agagatctgg tgttcgggta aaagatctat
     2521 taaaacgaac tcagtgtttg caagtttctt tctgaaattg ttccgaaacc caaaagagtt
     2581 tcctacaacg acgatgtgcg gagatagctt tttatatagt ccaacatagt aatcttcctc
     2641 gttttctgct aacccttcta ttgaaaagac tatatcaaca tctttatggt tgtttaccat
     2701 ctcagctcta agcgagtgct gatttaccgg tcttctctcc cctttaaatt tccttacttt
     2761 ttcgtcagaa tctacgccta ctattaatac gtcgcccagt ttttttgctt tattaaatag
     2821 gtatgagtgt ccggaatgaa atagatcgaa gcttccgtgg gtgtatatta ttttcttttt
     2881 tcttcttcta aggtcttgtg agatataaac aacggtatca aggtcgagta tatatcccat
     2941 gagaaaatta tataaataaa gaatttacac ttcaactttt ctatagtgct ataatcattc
     3001 agcatgttta aaaaattttt cgatacaaat tcaaaattgc ttgccaagca agcccccata
     3061 ctgagta
//
LOCUS       DAOSWN010000065         2821 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_26791_length_2821_cov_4.912871, whole genome shotgun sequence.
ACCESSION   DAOSWN010000065 DAOSWN010000000
VERSION     DAOSWN010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26791_length_2821_cov_4.912871"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..1157)
                     /locus_tag="PLF29_03190"
     CDS             complement(162..1157)
                     /locus_tag="PLF29_03190"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HXK52966.1"
                     /translation="MDKVTDNNSEVYKHFSESERDNLAVMLAEGKKQVDIAKKMGRHP
                     STISREIKRNGSPINKTQYRANRAQMRADKRKTESHSKTRLKNPKVRSYVIKKLKEGL
                     TPEQIAGRLKIDHPGLKTNYESIYLFIYKDRPDLVEYLVRGRRKRQKRGIKSGKRMVK
                     LPNRTMIDKRPAEINEKTEYGHWEADTVVSRQSKYALAVVRERRLQVMFIRRIDSKTA
                     NNMRQAVIDMLKDIPAEYRKSITFDNGLENAQHEEIAKQLSVQTYFCHPYHSWEKGGV
                     ENGIGVIRRFLPKKTDFALISWQQIRSIENRLNNRPRKTLNYLSPLEVFKIALSH"
     gene            1448..1657
                     /locus_tag="PLF29_03195"
     CDS             1448..1657
                     /locus_tag="PLF29_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52967.1"
                     /translation="MKKLLSAFFITTGLLVVSFTAVGQITKQVTEADIKECIAKKEKE
                     ARAKGYPLTDPFATRMQCINELKGR"
     gene            1660..2016
                     /locus_tag="PLF29_03200"
     CDS             1660..2016
                     /locus_tag="PLF29_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52968.1"
                     /translation="MKKILISSIAFLCFTLFATGYLESAPGDMVYADKYPQDAVLINL
                     SKKIVQIHRRGNDGRYTLQTTYRIISITDGNLNGIPTKVVRTKLNNNSYQDTFWTFKR
                     NWSAVYFYKVWVKRVQ"
     gene            complement(2308..>2821)
                     /locus_tag="PLF29_03205"
     CDS             complement(2308..>2821)
                     /locus_tag="PLF29_03205"
                     /inference="COORDINATES: protein motif:HMM:NF016847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXK52969.1"
                     /translation="NGFHVFFQPIEGDANEVLFRTAEYIATGYFHNSQITAIDHVKKT
                     ITFKYKSWVDRGTREKSFKTRTMNIHSFMARMLFFLPEKHRKMIRYYGIYAHKLDEKI
                     KKIETRTWAKAIENSFQKKPEVCPDCLKYMVKDTVYSFRADNAMKMLIKTHAIIGGYF
                     MPMIKQVRSP"
BASE COUNT          775 a          573 c          485 g          988 t
ORIGIN      
        1 agtaatagca tcatctttta ttgttaccaa catactttct ccgtatttag taaccagagg
       61 ctatattatc agtatatgta cgatgctgac aatcttgatt gtaagccaat ttttactgaa
      121 aattggaaaa tataatattg cgaccctgat tactcctgca ttcaatgact aagcgcaatt
      181 ttaaacactt caagtggaga gaggtagttt agagtttttc tcggtcggtt attcaacctg
      241 ttttctattg aacgaatctg ctgccaagaa atcaatgcaa aatctgtttt ctttggtaaa
      301 aatcttctaa tcactccaat cccattctct actccaccct tttcccagct atgatatgga
      361 tgacaaaaat atgtttgaac actaagttgt tttgctatct cttcatgctg tgcattttcc
      421 agtccattat caaatgtgat cgactttcga tattctgctg gaatatcttt cagcatatca
      481 ataacagctt gtctcatatt atttgcagtt ttgctatcaa tccttcttat gaacataacc
      541 tgaagtcgcc gctctctgac aaccgccaat gcatatttgc tctgacgaga aaccactgta
      601 tctgcttccc aatgtccata ctctgttttt tcattaattt ctgctggccg tttatctatc
      661 atcgtacgat tgggtagttt aaccattctt ttccctgatt ttatccccct tttctgacgt
      721 ttgcgcctcc ctctaactag atattcgaca aggtctgggc gatctttata gatgaacaga
      781 tatattgatt cataattagt ttttaatccc gggtgatcaa tcttaagtct gccagctatc
      841 tgctctggtg ttaacccctc tttcagtttc tttataacat agcttcttac cttcggattt
      901 tttagccttg ttttagaatg cgattccgtt ttccttttgt ctgctcgcat ttgtgctcga
      961 tttgcccggt actgcgtttt attgatcggc gagccatttc ttttgatctc cctggagatt
     1021 gtcgatggat gtctacccat tttttttgct atatcaacct gttttttccc ttcagccagc
     1081 attacagcaa gattatccct ttcagattcg ctaaaatgtt tgtagacttc tgaattatta
     1141 tcggttactt tgtccatgaa gaggctttct cctttgtttg gtttgtcgtg aattaacctt
     1201 aggaggttgt cctcttcttt taaactactt ttctataaaa attgcacttt tacattgaag
     1261 atgggaaaaa ctatttgaca attatttttt tttgtaaata tttgcatcaa tctgatacag
     1321 ataaaatcaa ggcttattct tggcataggg ctatcgcata ggtccctgga tagatcttca
     1381 aacctctttt tgtgtgattg ttttctttat aattttcagg aataaccgcc aactaaagga
     1441 gaaaaccatg aaaaaactac tatctgcatt ttttatcacc accggactac tggtagtatc
     1501 attcactgct gtaggacaga ttaccaaaca ggtaacagag gcagacatta aggaatgtat
     1561 agccaaaaaa gagaaagagg cccgcgctaa aggctatcca cttacagatc catttgctac
     1621 cagaatgcaa tgtattaacg agcttaaagg tagataatca tgaagaagat actcatctct
     1681 tctattgcct tcctgtgctt tacgcttttc gctaccggct atcttgagtc tgcgccgggt
     1741 gatatggttt atgcggataa atacccacag gatgcggtct taatcaatct ctctaaaaag
     1801 attgtgcaga tccatagaag ggggaacgac ggtagatata cgctgcaaac cacctaccgt
     1861 attatcagca ttaccgatgg caacctcaat ggtataccca ccaaggttgt aagaaccaag
     1921 cttaataaca atagctatca ggacacattc tggacattca aaagaaactg gagtgctgtt
     1981 tacttctaca aggtctgggt taagagggtt cagtaaaacc ccctcttgct tttgtgatct
     2041 gatcacagaa cctttatctt tttaggaaac tgcgatataa cacagagagt tttaacgtga
     2101 cgttctgtgt tataaaggat cataatgata cttatcagaa tgtgtaaatg tatctttctt
     2161 gacaatattt cttcaatacg acctgtttaa attaaatttt gcgcttcgcg caaatgttaa
     2221 aaaaacagct tttcctatga ttgtttacta acagccattc aaaaaaataa gtcaagacct
     2281 ttatctcttc taaaatttga aaatttttca aggcgatcta acctgtttaa tcatgggcat
     2341 aaaatacccg ccaattatag cgtgggtttt aatgagcatc ttcatcgcgt tatccgcacg
     2401 aaatgaatat accgtatctt taaccatata ctttaaacaa tcggggcata cttcaggctt
     2461 tttttgaaat gaattttcaa ttgcttttgc ccaggtacgt gtttcaattt tctttatttt
     2521 ttcatcgagt ttgtgtgcat agattccata gtaccggatc atctttctat gcttttccgg
     2581 caggaaaaaa agcatgcgtg ccataaacga atgaatattc atggttctgg ttttaaaact
     2641 tttctcccta gttcctctgt caacccagct tttgtatttg aaagttattg tttttttgac
     2701 gtgatctata gctgtgatct ggctgttatg aaaataaccg gtagcgatat attcagcggt
     2761 tctgaagagg acttcatttg catcaccttc aatcggctgg aaaaatacgt ggaagccgtt
     2821 t
//
LOCUS       DAOSWN010000066         2817 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_26855_length_2817_cov_4.618755, whole genome shotgun sequence.
ACCESSION   DAOSWN010000066 DAOSWN010000000
VERSION     DAOSWN010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26855_length_2817_cov_4.618755"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="PLF29_03210"
     CDS             <1..835
                     /locus_tag="PLF29_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007050991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HXK52970.1"
                     /translation="VYTSATPSAWEIEASKDRIVEQIIRPTGLIDPRITVLNTEGQIE
                     DLFSRILEKTSLGERVLVTTLTKRMAEELATYLVDKGIKVTYLHSDIETLERSNILDD
                     LRKGNYDVLVGINLLREGLDLPEVSLVAILDADKEGFLRSESALIQTMGRAARHINGE
                     VVMYADNVTKSMERAIGEVNRRRAIQEKYNIEHNITPVSISKPIRAKLYEEVIDVTES
                     DRKKTLSDIDYLQLPPKFLKKELLKLTKEMQYEADSLNFEKAAVIRDQIRKIKKQANL
                     T"
     gene            926..>2817
                     /locus_tag="PLF29_03215"
     CDS             926..>2817
                     /locus_tag="PLF29_03215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HXK52971.1"
                     /translation="MAKKEKIEKTDLITHILIGIIELLVIIGYVFLWPIERSICFIAK
                     IFNSIKKTSLKTAKNFISIPKTLTKPLFLIFSVFGRVRRTITRIVVFSFRADLLGPAR
                     SELSRYRSIFVKASFSRIVGKILFTKHKSKRSSKTNKPTQAAKTKKEPAGSKNIPLDK
                     SLKKKSVKVSHPKRTALRKFLLSPFATFFYGGLFVLLFIVAPAMTYLWYKQLPQPVLL
                     EQRAYNSSTRILDRKGRLLYEIYVDKNYAPVRLEDIPQVVIDSTLAIEDSEFYSHHGF
                     RVTSIVRAAKATLLEDDLQGASTITQQLVKNVLLTPERTISRKVKELVLSILVEQKYS
                     KNEILEFYLNNIPYGGTAYGVQAAANKYFGKDVSDLDLAEASLLAGLPSAPSVYSPVS
                     GNYDLAKERQKQVLDRMVELGFIRSADAEVAYAQELVFASQQEYIRAPHFVEFVRKSL
                     YEIFGQRMVDFGGLSVTTTLDLDLQDQVQEIVREEVDAGARFGFSNAAAVVVDVRTGG
                     ILAHVGSYDYFLGSDGKFDVATAYRQPGSSVKPITYALALENGMTAASTVVDAPITYQ
                     SYGQKYSPKNYDNTYHGVVTLRTALANSYNIPAVKLVSQLGRDNMVQLGHDIGLSNWK
                     VDNTYG"
BASE COUNT          900 a          523 c          607 g          787 t
ORIGIN      
        1 agtatataca agtgcaacgc cgtccgcttg ggagattgag gcatctaagg atagaatagt
       61 agagcaaatt attaggccca ccggattgat tgatcccaga atcactgttt tgaacacaga
      121 agggcagatc gaagatctat ttagtaggat actagaaaaa acttctttag gagaaagagt
      181 tttagttaca actttaacta agaggatggc tgaagagttg gcaacctacc tcgttgataa
      241 aggcataaaa gtaacatact tacattcgga tattgagact ttggaaagat ccaacatttt
      301 agacgaccta cgaaagggta attacgatgt tttagttggt atcaacttat tacgagaagg
      361 tttggacttg ccggaagttt ctttggtggc aatattagat gctgataaag aaggtttttt
      421 gagatcagaa agcgcactga ttcagaccat gggccgtgcc gcaaggcata taaacggtga
      481 ggtagtgatg tatgcagata atgtcacgaa aagcatggag cgtgctatcg gagaagtaaa
      541 cagaagaagg gccatccaag aaaaatataa tattgagcat aacatcacgc ctgtttcaat
      601 ctcaaagcca ataagggcaa agctatacga agaggtcata gatgttacag agtcagacag
      661 aaaaaaaacg ctatcggata ttgattattt acaactgcca ccaaaattct taaaaaaaga
      721 gcttttaaaa ctcacaaaag agatgcagta cgaggccgat agcttaaatt ttgaaaaggc
      781 ggctgtcatt cgtgaccaaa tacgaaagat aaaaaaacag gctaatttga cataacctgt
      841 agttgcaatt tataatacgc gagtaccatc ttaagatgat taatttaagc agtaataccg
      901 gtacaagtta caggacaggt acttaatggc caaaaaagaa aaaatagaaa aaaccgactt
      961 aataactcat atactaattg gaatcatcga acttttggtt attattggtt acgtttttct
     1021 atggcctatt gaaagatcga tttgttttat cgctaagatt tttaactcga taaaaaaaac
     1081 ctcattaaaa acagctaaaa actttatatc aatacctaag accctaacca agcctttatt
     1141 tttgatattc tctgtctttg gccgtgtccg tcgcacaata acaaggatag ttgttttttc
     1201 ttttcgtgca gatctgcttg ggcccgcacg ttcagaactt agcaggtaca gaagcatttt
     1261 tgtaaaagct tctttttcta gaattgttgg taaaatactg ttcacaaaac ataaatctaa
     1321 aagatcctca aaaacaaata agccgactca ggctgctaaa actaaaaaag agcccgcagg
     1381 ttccaagaat atccccctcg acaagagtct gaaaaagaag agtgttaaag tcagtcaccc
     1441 aaaacgtact gctttgagaa aatttcttct aagcccgttc gcaacatttt tctatggagg
     1501 actatttgtg cttttgttta ttgttgctcc agcaatgact tatttatggt ataagcaact
     1561 tcctcagcct gttttactgg agcagcgggc gtataatagc tcgacgcgca tattagatcg
     1621 taagggccgt ctgttatacg aaatttatgt agataaaaac tatgcccccg tgagacttga
     1681 agatattcct caagttgtaa ttgattccac attagctata gaggattctg agttttatag
     1741 ccaccacgga tttagagtaa ccagtatagt gagggccgca aaggccacct tacttgaaga
     1801 cgatctccag ggagcttcca caattaccca acagctggtt aaaaatgttt tattaactcc
     1861 cgaaagaaca atttcaagaa aagttaaaga gttggtattg tccattcttg ttgaacagaa
     1921 gtattcaaag aatgaaatac ttgaatttta cctaaacaat attccctatg ggggcacggc
     1981 ttatggggtg caggctgcag cgaacaaata ttttggcaaa gatgtttctg atcttgactt
     2041 ggctgaggca agcctgcttg caggcttgcc ttcagctccg tctgtttact ctccggtttc
     2101 cggaaattac gatttggcta aagaaaggca aaaacaagta ctagatagga tggtagagct
     2161 ggggtttatt agaagtgccg atgcggaggt cgcctacgcc caagagcttg tttttgcctc
     2221 acagcaagaa tacattaggg ctccacattt tgtagagttt gtcagaaagt cactctatga
     2281 aatatttggg cagcgcatgg ttgacttcgg aggtttatct gtaaccacaa ctcttgatct
     2341 tgatcttcaa gatcaagttc aagaaatagt tagagaagaa gttgatgccg gggcacgttt
     2401 cggcttttca aatgctgcgg ccgtagtggt cgacgttcgc acaggcggga tactggcaca
     2461 tgtcggctcg tatgattact ttttaggaag cgatggtaag tttgatgtcg ctacagccta
     2521 tcgacagccc ggatcctctg ttaagccgat tacatatgct ttggcccttg aaaatggcat
     2581 gacggctgca agcactgttg tggatgcccc aataacgtat cagtcatacg gtcaaaaata
     2641 ttctccaaag aactatgaca acacatatca cggggttgtg actttgcgta ccgccttggc
     2701 taattcttat aatataccgg cagtaaaact agttagtcag ctgggccgag ataatatggt
     2761 tcaacttggg cacgatatag gtttaagcaa ctggaaggta gataatacat atggctt
//
LOCUS       DAOSWN010000067         2693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_28839_length_2693_cov_4.513268, whole genome shotgun sequence.
ACCESSION   DAOSWN010000067 DAOSWN010000000
VERSION     DAOSWN010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28839_length_2693_cov_4.513268"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1542
                     /locus_tag="PLF29_03220"
     CDS             <1..1542
                     /locus_tag="PLF29_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52972.1"
                     /translation="YTADSSLRGDLKAGNITTEPTYASPGTWELGDMEDGEVVTLTLK
                     AKTAISVDPGLYKDLTWTQGSDSNENEILAQAETEGYVDENFSGTEVGVKEKPQPDEA
                     KTEVKVNKKEIIEEVLGASTERGEVLGLPATGTITAILATLLVSLASGIILIVFGLLR
                     KNKKMSLTRTARVTLISLLVLLAAGAMNFNKVDAAAALRVEDPISPANSEFSITFVLL
                     DTESRPNTNVECYVKKPTSADYIKYQEVSLADGGNTDSCLVNNSVLTEDGTYYFNVKA
                     VFSDETSITSESVSVKYDSKGPGKPRYIKKDKKNSCEYEVKFKTSSEGDTNYVEVYRS
                     FNKEFTVNDSSKIKTISIGNDATYEFTDNLPSDKCGKKPYYAVRAFDEAGNPSNVRVE
                     EITEETIIEKTVNSDSDSEVTTTEGQFIGAAGGLQSLTGTAGEQDQNQTGADQDQGDG
                     AVLGTNEDEGNGNIRTDRERNSSTLLAKLVNALTSPVIYIPVIILAVLGLYLGAKKRL
                     KNSQK"
     gene            1505..2233
                     /locus_tag="PLF29_03225"
     CDS             1505..2233
                     /locus_tag="PLF29_03225"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HXK52973.1"
                     /translation="MGQRNDLKIVKNKSNGNGSRRLLHTGAALFSLSLIGLALLFLPV
                     LGEELNYLVNKKGYGANIQTISKDSTNKNINDFLSNFQPYNEDSIYIPKIGAKAKILK
                     NIDPFNSEEYGTALKYGVAHAKGSDYPTSGLSLGTNTFVFAHSTDGLINVNKYNAIFY
                     LLYKLAPTDEIYLKFSESQKNFINKYSVSEVKIVGPKELAYLKTLEGYEDKNTLTLMT
                     CWPAGTTLKRLVVIATLSETIAVE"
     gene            complement(2289..>2693)
                     /locus_tag="PLF29_03230"
     CDS             complement(2289..>2693)
                     /locus_tag="PLF29_03230"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HXK52974.1"
                     /translation="IISRSGWILPLLGNESNVNHKKNLLEKKMEYKKLKRRAEEILSA
                     RLRHFNAIYGFRFKSVKIRNQSSRWGSCSSSGSINFNYRLILLDQELIDYVVVHELCH
                     LREMNHSPAFWRLVEKSIPNYKVLKKKLARLS"
BASE COUNT          938 a          545 c          550 g          660 t
ORIGIN      
        1 tatactgcag actcgagttt aaggggagat cttaaagccg gaaatattac taccgaacca
       61 acttatgcct ctccgggaac atgggaacta ggagacatgg aggacggaga agttgtaact
      121 cttacgctaa aggctaaaac agcaatttca gtggaccccg ggctgtataa agacctcaca
      181 tggactcaag gttccgacag taacgaaaac gagatcttgg cacaagcgga aaccgagggc
      241 tacgtagacg agaacttttc gggcaccgaa gtgggagtaa aagaaaagcc tcagccggac
      301 gaagcgaaaa ctgaagtaaa ggttaacaaa aaagaaataa ttgaagaggt tttaggagca
      361 tctacagaac gtggggaagt tttggggcta cctgcaacag gaacaataac cgccatacta
      421 gcaaccttac tagtgtcctt agcctcagga ataatattaa tagtatttgg cctgcttaga
      481 aagaataaaa aaatgtcatt aacaagaact gcaagagtca cgctcatatc cttgttggtc
      541 ctgctggccg caggcgcaat gaacttcaac aaagtagacg cagccgccgc actaagagta
      601 gaggatccaa taagccctgc aaacagtgag ttttcgatta catttgttct gttagataca
      661 gaaagcaggc ccaacacaaa cgtagagtgc tatgttaaaa aaccgacatc ggcagactac
      721 ataaaatatc aagaggtgtc tctcgccgac ggcggcaaca ccgactcttg cttagttaat
      781 aactcagtac ttacggaaga cggaacatac tactttaacg tcaaagccgt gttttctgac
      841 gaaacttcaa taacttcgga aagcgtcagt gtgaaatacg acagcaaagg ccccggaaaa
      901 ccaagataca tcaaaaagga taaaaaaaat tcctgtgaat atgaggtaaa atttaaaact
      961 agcagcgaag gagacacaaa ctatgtagag gtgtatagat cttttaacaa agagtttaca
     1021 gttaatgaca gctctaagat taaaactatc tcaataggta acgacgctac atatgagttt
     1081 accgataacc ttccttccga taaatgcggt aaaaaacctt actacgcagt aagggctttt
     1141 gacgaagccg gaaatccttc taatgtcagg gtggaggaaa taaccgaaga gaccataatt
     1201 gaaaaaacag ttaatagtga cagcgatagc gaggttacta ctaccgaggg acaatttatc
     1261 ggggctgcag ggggcttaca gagcctcaca ggaacagcag gtgaacaaga tcagaatcag
     1321 acgggagctg accaagatca aggagacggt gctgttctag gaactaacga ggacgaggga
     1381 aacggcaaca taagaacaga tcgagaaaga aattcctcaa cactcttagc taaactggta
     1441 aatgctctta cttctccggt aatctatatt ccagtaataa ttttggcagt tttaggctta
     1501 taccttgggg caaagaaacg acttaaaaat agtcaaaaat aaatcgaatg gtaatgggtc
     1561 gaggcggctc ctgcacacag gagccgcact cttttcactc tcacttatag gactggcgtt
     1621 attgttcctg ccggttcttg gcgaggagct taactacctt gttaacaaaa aaggttacgg
     1681 tgcaaacata caaactatct ctaaagactc aacaaataaa aacattaacg attttttaag
     1741 taatttccag ccatacaacg aagattcaat ctacataccc aaaattggcg caaaagcaaa
     1801 aatattgaaa aatattgatc cgtttaattc tgaggaatat ggaacggcct taaaatacgg
     1861 agtagctcac gccaaaggca gtgattatcc aacctcgggg ctgtccctcg gtactaacac
     1921 cttcgttttt gcgcattcaa cagacgggct tattaatgta aataaataca atgcaatatt
     1981 ctatttgctc tacaagcttg caccgactga tgaaatatat ctaaagttct cagaatcaca
     2041 aaaaaacttt ataaataagt attcggtttc tgaggttaaa atagttggcc caaaagaact
     2101 agcatatcta aagacgttag aaggctacga ggataaaaac accttaacac taatgacatg
     2161 ctggccggcc ggaacaacat taaaaagact tgttgtaata gcaactctaa gcgaaacaat
     2221 agctgttgag taaacttgcc agacgagaag aaacaaactt cagaaaaaat ctcagactaa
     2281 aaatttactt atgacaggcg ggctagtttc ttttttagca ctttataatt tggaatactt
     2341 ttttcaacta atctccaaaa agcaggggag tgattcattt cgcgcagatg gcataattcg
     2401 tgaacaacaa cgtaatctat caactcctga tctaataata tgagcctgta attaaaattt
     2461 attgagcccg acgaagaaca actaccccac cttgatgatt gattccggat cttaactgat
     2521 ttgaatctaa atccatagat cgcgttaaaa tgccgcagtc gggcagacag tatctcctcc
     2581 gcacgtcttt taagtttttt atactccatc ttcttttcta ataaattttt cttatgatta
     2641 acatttgatt catttcccag taaaggcagg atccacccgg atctcgatat aat
//
LOCUS       DAOSWN010000068         2621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_30106_length_2621_cov_4.196804, whole genome shotgun sequence.
ACCESSION   DAOSWN010000068 DAOSWN010000000
VERSION     DAOSWN010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30106_length_2621_cov_4.196804"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="PLF29_03235"
     CDS             <1..463
                     /locus_tag="PLF29_03235"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HXK52975.1"
                     /translation="LVQYKKGGLDMNSGVLYVIASSILFAVFQVASANLSKVISTGAY
                     LLMAYIGPSLIVGILYLKRIINDLHLLKQQIKESFLKTLFASGTSMLYFVFSYLAYRY
                     APDRGVVVVLLTAQVVLSVIFGIIFLKEKDNQLKKVIAGALAFAAGIMIKS"
     gene            complement(753..998)
                     /locus_tag="PLF29_03240"
     CDS             complement(753..998)
                     /locus_tag="PLF29_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52976.1"
                     /translation="MKNKEPTHICANCGFKGRPVKQTKGSFALELLLWIVFLIPGVAY
                     TIWRMTTTQEVCPKCKTPNMLPLDTPKGRELEQSFLA"
     gene            1260..2123
                     /locus_tag="PLF29_03245"
     CDS             1260..2123
                     /locus_tag="PLF29_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52977.1"
                     /translation="MQSNILIAGYGVFGAGLVDHIESNHKNIKIVKIIEPNRHKRDIL
                     LKRGYKTCESPHELSSHDAHEAKVVIDCSPRGEGSKNKPIYSNIGLRAIFQNGEEDRS
                     IGALYYPGSTNINKHEHFLMPLCSGISVLKVLSALKKNKMSLPYKILGTHSKVSNTSR
                     MLMPNNPNANSQILELFGVNARMDIVYLRGEPYQNKFTYYGSVQLQYKEKAPSRKDFI
                     NSIAEHPWIDIISDKSDLLTNLSIKDKTAIVKESFYSDGKTISFMGLSSTPDVNYPIV
                     IQAVLDIYSMR"
     gene            complement(2189..>2621)
                     /locus_tag="PLF29_03250"
     CDS             complement(2189..>2621)
                     /locus_tag="PLF29_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52978.1"
                     /translation="YVSARKRDISERDLRAEREKQLKSSQDRGDDNDIFPPGFLASTL
                     LPKEPLYYHIVHVKNGKVFLVCFDPRLSTTYTVDKGGKESWPLLVQLKPDFNAQLKHG
                     LFVQAHDIRVPSGATRSPSPHPDYDRCSTVAVRRAALIFRE"
BASE COUNT          869 a          513 c          502 g          737 t
ORIGIN      
        1 tcttgttcag tacaaaaaag ggggactaga tatgaattcg ggggtacttt atgtaatagc
       61 ttcatcaata ctattcgctg tatttcaagt tgccagtgca aatctatcta aagtaattag
      121 cacaggagca tacttactta tggcatatat agggccgagt ttgattgttg gcatattata
      181 cttgaaacgt attatcaacg atcttcacct cttaaaacaa cagattaagg agtcgttttt
      241 aaaaactcta tttgcaagtg gtaccagcat gctttatttt gtattttctt atcttgcata
      301 tagatacgct cccgatagag gtgttgtcgt ggttctactt actgcacagg ttgttttatc
      361 agttattttt ggaattatct tcctcaaaga aaaagataat caattaaaga aggttatagc
      421 aggagcgcta gcttttgcag caggaataat gataaagtcg tagcccccag cctgtgctat
      481 tgtctcattt gcatcttgga gcatgaaaca ctccatgacc ttactgctac accaccccta
      541 actttaaacg agaataacta aagtgcacgg aaaataaacc accaccagct tacaaaggtt
      601 gtttgccgta atatctttgc gcggcagagc tcaacacgtt gggtatactg cgtctactag
      661 cttaaacaag tagttttaag taacaaaatt ataaacaagc tacccttaat gagaaaagta
      721 gacgtcaata ttccaaatac aaagcgtgaa tattaagcca aaaaactttg ttcaagctct
      781 ctacctttag gggtgtctaa ggggagcatg ttaggcgttt tgcatttagg gcaaacttct
      841 tgggttgtag tcatcctcca tatggtataa gcgactcctg gaattaaaaa tacaatccac
      901 aataaaagtt caagagcaaa ggatccctta gtctgtttaa cgggcctgcc tttaaagccg
      961 caattggcgc aaatgtgtgt aggttctttg tttttcataa cgtaagttta ctacaagacc
     1021 tgctcagtaa caagcctcga tgagccgact ataaagtaag cacccacgaa tacccaaata
     1081 gtaagatgac acctaaaaaa ctacaaaaga tttatttttg atggtacaat acgattatgc
     1141 tagttgagaa gaaattaata ttctcattga cctttttctt tggcctctta taggggtcac
     1201 aaatcatcaa gcacaactta atacgttaca cataattaaa ttaaatattt aaggataaaa
     1261 tgcaatcaaa catactaatt gccggatatg gggtcttcgg agcaggactt gttgatcaca
     1321 tagaaagcaa tcataaaaat ataaaaatag ttaaaataat tgagccaaat agacacaaaa
     1381 gagatattct tttgaagaga gggtacaaaa cctgtgaaag cccgcacgaa ctttcttcac
     1441 acgatgctca tgaagcaaag gtagtgatag attgttcacc aagaggtgag ggctcaaaaa
     1501 acaaacctat ttatagcaat attggtttaa gagcaatctt tcagaatggg gaagaggatc
     1561 gttctatagg agcattgtat tacccgggaa gcaccaacat taacaagcac gagcattttc
     1621 tcatgcccct gtgctcagga atttccgtac ttaaagtatt aagtgcattg aagaagaaca
     1681 aaatgtcgct accatacaaa attttaggaa ctcattctaa agtatcaaac acgtcacgaa
     1741 tgctcatgcc caacaaccct aacgctaaca gccagatact ggagctgttt ggggtaaatg
     1801 ctcggatgga cattgtttac cttagagggg aaccttacca aaacaaattt acttattacg
     1861 ggagcgtaca gctgcaatac aaagaaaaag ctccgagtcg aaaagatttt attaattcta
     1921 tcgctgaaca tccctggata gatatcatct cggacaaatc tgatttactc acaaatctga
     1981 gcataaaaga taaaaccgct attgtgaaag aaagttttta ctcagacgga aaaacaattt
     2041 cctttatggg actttcaagt acgcctgatg taaactaccc gatcgtaata caagcagttt
     2101 tagatatata ttcaatgcga tagaagccta ttaattcgag cctacctttc ttctttgttg
     2161 gggaggggat ctttgcgtta gaaaaaaact attctcgaaa aattagggct gcccgccgaa
     2221 cggcaacagt actacaacgg tcatagtcag gatgaggact aggcgagcga gtcgcaccgg
     2281 aaggcactcg aatatcatgc gcttgaacaa aaagaccatg cttaagttga gcattaaagt
     2341 ctggcttcag ctgaaccaac aagggccaag actccttacc ccctttatca actgtgtagg
     2401 tggtggacaa acgcggatca aaacatacta aaaaaacttt gccattttta acatgaacta
     2461 tgtggtaata aagaggctct ttgggtaata gcgtggaagc aagaaaccct ggaggaaaaa
     2521 tgtcgttgtc atctcctcta tcttggctcg atttaagttg tttctcacgc tcagctctca
     2581 aatctctttc acttatatcc ctctttctag cagacacata a
//
LOCUS       DAOSWN010000069         2620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_30123_length_2620_cov_4.458869, whole genome shotgun sequence.
ACCESSION   DAOSWN010000069 DAOSWN010000000
VERSION     DAOSWN010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30123_length_2620_cov_4.458869"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /gene="rplP"
                     /locus_tag="PLF29_03255"
     CDS             <1..220
                     /gene="rplP"
                     /locus_tag="PLF29_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012459018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HXK52979.1"
                     /translation="VWIRVFPDKPITKKPNETRMGGGKSPASHYAAVVKPGKILFEIA
                     GVTEEVAKAAFKRAADKLPVKTRFVSAE"
     gene            228..428
                     /locus_tag="PLF29_03260"
     CDS             228..428
                     /locus_tag="PLF29_03260"
                     /inference="COORDINATES: protein motif:HMM:NF013027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HXK52980.1"
                     /translation="MTNTKLDLKNKNREQLLEMLKVAKKDKEDFVANLLKNKEKNVTK
                     LRQLRKNIARIQTAVNALEEKN"
     gene            430..663
                     /gene="rpsQ"
                     /locus_tag="PLF29_03265"
     CDS             430..663
                     /gene="rpsQ"
                     /locus_tag="PLF29_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017692084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HXK52981.1"
                     /translation="MPKKIFVGKVIADKMQKTVVVAVESPKRHPFYGKMLKNTMKYSA
                     RNIVNAKVGDNVKIVESRPYSKNVSWEVTEVLE"
     gene            660..1027
                     /gene="rplN"
                     /locus_tag="PLF29_03270"
                     /pseudo
     CDS             660..1027
                     /gene="rplN"
                     /locus_tag="PLF29_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011994288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
     gene            1024..1329
                     /gene="rplX"
                     /locus_tag="PLF29_03275"
     CDS             1024..1329
                     /gene="rplX"
                     /locus_tag="PLF29_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020310051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HXK52982.1"
                     /translation="MKIRVGDTVQIITGKDKGKKGKVLKTFRNEDKVVVEGINLVTKH
                     LKPGPQVKEGGIVSIEKAIHVSNVMYFDDKANRPTKIKMQADGKKYIRASKASGDKI"
     gene            1331..1873
                     /gene="rplE"
                     /locus_tag="PLF29_03280"
     CDS             1331..1873
                     /gene="rplE"
                     /locus_tag="PLF29_03280"
                     /inference="COORDINATES: protein motif:HMM:NF000585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HXK52983.1"
                     /translation="MSLRDIYKDKIANELVKDLGLTNLMQAPKLSKIVINAGIGPFRE
                     SKEAVDSFVEELSAIAGQKPIASSAKKSEAGFKIRQGDVVGYSVTLRDARMWAFFEKF
                     VNIVMPRVRDFNGLSLASFDGSGNYSVGIKEHTVFPEVNPNKVKGIRSIQVTFCIKNG
                     NKQKSTALLKKLGMPFKKEA"
     gene            1875..2060
                     /locus_tag="PLF29_03285"
     CDS             1875..2060
                     /locus_tag="PLF29_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HXK52984.1"
                     /translation="MAKKALIAKQKRPAKFSTREYNRCELCGNPRGFMRRFSICRKCF
                     RELAHKGEIPGVRKASW"
     gene            2064..2468
                     /locus_tag="PLF29_03290"
     CDS             2064..2468
                     /locus_tag="PLF29_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HXK52985.1"
                     /translation="MSQDLLSNMLSSLKNASMAKNKFIETVHSKECEAVAKVLKERHF
                     LEEVKTFKEKDSSFKRLHLALAYKDGMPVLKNAKRVSKPGRRVYLGHAGLEPIYGNQK
                     TVIVSTSRGVMTSVDAKKKKLGGEVICEVSNV"
     gene            2461..>2620
                     /gene="rplF"
                     /locus_tag="PLF29_03295"
     CDS             2461..>2620
                     /gene="rplF"
                     /locus_tag="PLF29_03295"
                     /inference="COORDINATES: protein motif:HMM:NF012567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HXK52986.1"
                     /translation="MSRIGKKEITIPASVKVTYEKGVVSVSGPKGNLSHEIRPEIGVQ
                     ISGDNLTTV"
BASE COUNT          979 a          386 c          615 g          640 t
ORIGIN      
        1 agtatggatc agggttttcc ctgacaagcc gatcacaaag aagcctaacg aaacaagaat
       61 gggcggaggt aagtcccctg cgtcacatta cgctgcagtt gtgaaacccg gaaaaatact
      121 ttttgaaata gcaggggtca cagaagaagt agccaaagca gcattcaaga gagccgcaga
      181 taagttaccg gtaaaaacca ggttcgtgtc tgctgagtag gagtattatg actaacacta
      241 aactagattt gaaaaataaa aacagagagc agctgcttga aatgttaaaa gttgcaaaaa
      301 aagataaaga agattttgta gcgaatcttt tgaaaaataa ggaaaaaaat gttacaaagc
      361 ttaggcaact cagaaagaat attgcaagaa ttcagaccgc agtaaatgcg ctagaggaga
      421 aaaattgata tgccaaaaaa aatatttgta ggaaaagtca tagccgataa aatgcagaaa
      481 acagttgtgg ttgctgtgga gtcgccaaaa agacacccgt tttatggaaa aatgcttaaa
      541 aatactatga aatacagtgc aagaaatatt gttaatgcga aggttggaga taacgtgaaa
      601 attgttgaat ctaggcctta cagcaagaat gtttcctggg aagttacgga ggtattagaa
      661 tgattcacat aggatctgta attaattcag ccgataactg cggagcaaaa agcttgaaag
      721 ttataggtat accgggaaac tctaagcaga aggttgctaa ggtcggagat attattacag
      781 tcgtagtgaa cggcgcatcc tctaaaggcg tggtgaaaga tcactcagta gttaaagcca
      841 taatagtgag aacccgaaaa gagtacagaa gaagtgacgg aacctacatt agatttagcg
      901 ataatgcagg tgtagttata aacaaagata gaacatggcc ggtactagag tttttggccc
      961 cgttgcaaga gaaattagag acagagggta cttaaaagtt gcctcattgg caaaggaggt
     1021 ctggtgaaga tcagagtagg cgatacagtt caaataatta ccggtaagga caagggtaaa
     1081 aaaggcaaag ttttaaaaac ctttagaaat gaggacaagg tggtagtaga gggaataaat
     1141 ctggtcacaa agcatctcaa gccaggccct caagttaaag aaggcggaat tgtgtcgatt
     1201 gaaaaggcga ttcacgtttc gaatgtaatg tattttgatg ataaagcaaa caggcctaca
     1261 aaaataaaaa tgcaggccga cggaaaaaaa tatataagag cctcaaaggc ctcaggagat
     1321 aagatttaaa atgagtttaa gagatatata taaagataaa atagcaaatg agttggttaa
     1381 ggatttaggt cttactaatt taatgcaggc accaaaactt tcgaagattg ttataaatgc
     1441 tggaatcggg ccttttagag agagcaaaga agctgttgat tcctttgtcg aagaattgtc
     1501 tgctattgca ggacagaagc cgatagcctc ctccgctaag aagtcagagg cagggttcaa
     1561 gataagacag ggagatgttg tgggttacag cgtgacactt cgagatgcga gaatgtgggc
     1621 tttttttgaa aaatttgtta atatcgtcat gccccgtgtt agagatttta atgggctaag
     1681 cttggcctca tttgacggaa gcggaaatta ctctgtggga ataaaagaac acacagtgtt
     1741 ccctgaagtt aaccctaaca aggtaaaagg aattaggagt atacaggtca cattctgtat
     1801 aaagaatgga aacaagcaaa aaagtacggc attgttaaaa aaattaggga tgccttttaa
     1861 gaaagaagcg taatatggca aaaaaagcat taatagcaaa acagaaaaga ccggcgaaat
     1921 tctcaacaag agaatacaac cgctgtgaat tatgtggaaa tccaagaggt tttatgagac
     1981 gattcagcat ttgtagaaaa tgttttagag aattagcaca caaaggtgaa attccgggtg
     2041 taagaaaggc tagctggtaa aacatgtcac aagatttatt atcaaacatg ctaagttctt
     2101 taaagaatgc ctccatggca aagaataagt ttattgaaac tgttcattct aaagagtgcg
     2161 aagcagtagc gaaagtactt aaagagaggc actttcttga agaggtaaaa acttttaaag
     2221 aaaaagactc ttcttttaaa agattacacc ttgctcttgc atacaaagat gggatgccgg
     2281 ttcttaaaaa tgcaaaaaga gtctcaaaac ctgggagaag ggtttatttg ggacacgcag
     2341 gtttagagcc tatatatgga aatcaaaaaa ctgttattgt atctacttcc agaggcgtaa
     2401 tgacctcagt agatgcaaaa aagaaaaaat tgggcggtga ggtaatttgt gaggtaagca
     2461 atgtctagaa ttggtaaaaa agaaataact atacctgctt ctgtaaaagt cacttatgaa
     2521 aaaggtgtag tttctgtgtc aggtcctaaa ggtaatttgt cacatgaaat aagacccgaa
     2581 attggagtac agattagtgg agataactta accaccgtag
//
LOCUS       DAOSWN010000070         2450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_33690_length_2450_cov_3.949896, whole genome shotgun sequence.
ACCESSION   DAOSWN010000070 DAOSWN010000000
VERSION     DAOSWN010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33690_length_2450_cov_3.949896"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..352
                     /locus_tag="PLF29_03300"
     CDS             104..352
                     /locus_tag="PLF29_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52987.1"
                     /translation="MSDKPVNTINMLERVAKLEERVDGGFGFVNKELKEIKENHLHQL
                     QADVSDLKIKVSTLAVKISIAAAVATIVGDFIFRLLFR"
     gene            complement(327..641)
                     /locus_tag="PLF29_03305"
     CDS             complement(327..641)
                     /locus_tag="PLF29_03305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HXK52988.1"
                     /translation="MTMKDKGSKKKPQAKMTVFGTAPVSSRGQIVIPAKLRKAQNINP
                     GDTLIFTGNPESGYFHVLNANSFKGFTNDLNNLVFNHKQEADLAKKEMKVKEPTEKED
                     GK"
     gene            943..1719
                     /locus_tag="PLF29_03310"
     CDS             943..1719
                     /locus_tag="PLF29_03310"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HXK52989.1"
                     /translation="MQKSKLPPSIRSISKSPIDQQKIINDIQQELINNIDKKYKLSSL
                     KFYKHKTHNYGVKTPLVRKIAIKYFREIKSLNKKQIYEICENLLKNRSNEEATISIQW
                     AGATSKDFTEKDFKIFESWLTKYIDNWSKDDDFCLHILNPLLKMYPNLIAHTKKWAMS
                     KNMWLRRASAVCMISSDKSMYVSEHSLNDIFEVSIKLMGDREDLVQKGYGWLLKAASI
                     RKQREVFDFIIENKLKMSRTSLRYAIEKMPPELRKKAMEK"
     gene            complement(1752..2177)
                     /locus_tag="PLF29_03315"
     CDS             complement(1752..2177)
                     /locus_tag="PLF29_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HXK52990.1"
                     /translation="MKSAKKSRALIIKDGMVLVLLKYKNGAVYLTIPGGTYEETDATL
                     EQTVLRELYEETSIQAKVVKFLFSAYDPLRKCEQSLYLCEYVSGEPELLDEVEIKHLK
                     ENPNLLVRPVWVIPSYFKNMEVHPEVLKDFFVSYLEQNS"
BASE COUNT          850 a          430 c          463 g          707 t
ORIGIN      
        1 cttcggtcaa tggactccga ttgttgtagc tttctggtca gttgttgtca acacagtcag
       61 aaaatggtta acagcaagtg aatatattga gaactaatgt cctatgagtg ataaaccagt
      121 caataccatc aatatgctcg aacgtgttgc caaactcgaa gaacgagtag atggcgggtt
      181 tggatttgta aataaagagt taaaggaaat aaaagaaaat catttacacc aattacaagc
      241 agatgttagt gacttgaaaa taaaagtgag tactttggcg gtgaagatta gcatcgctgc
      301 tgctgtggca acgatagttg gagattttat tttccgtctt ctttttcggt aggctcttta
      361 accttcatct cttttttagc aagatccgct tcctgcttat ggttgaagac aagattattc
      421 aaatcattgg tgaaaccctt gaaagaatta gcattcagaa catggaagta cccagactca
      481 ggattaccag taaagattag agtatcacca ggattaatgt tctgagcttt acgcaattta
      541 gcaggtatca caatttgacc gcgtgaactt accggggcgg tcccgaacac tgtcattttt
      601 gcttgtggct tcttttttga gcctttatct ttcatagtca tataaaacgg taactactcc
      661 accgagaaat tgttcctaca ataaagaata atctttaatg taggttacgg gtattataca
      721 atacatcgat ctcgagaagt tcgcaacatc agaaagtgac agaaacaacc ttgtatctac
      781 tagggtaaaa gaagtaaaat aaggatgtta ttgataggcg gcggcgaaag attcaatgcc
      841 aatatgttct ttaagaaaac taagctggag ttccaaacta ggctcgataa gaggtcttag
      901 gttcgagcaa agtacggtga ccagctccaa attctcctac atatgcaaaa aagcaaacta
      961 ccaccaagta taaggtcaat aagcaaatcc ccgatagatc aacaaaaaat cataaatgac
     1021 atccagcaag aactgataaa caatatagac aaaaaatata agctatcttc tctaaaattt
     1081 tataaacaca aaacacacaa ttacggagtg aaaaccccat tagtaagaaa aattgcaata
     1141 aagtattttc gagaaataaa aagcctaaat aaaaaacaaa tttatgaaat ttgcgaaaat
     1201 ctgttgaaaa atagaagcaa cgaagaagca actatatcca tacagtgggc aggagctact
     1261 tcaaaagact ttactgaaaa agatttcaaa atatttgaaa gctggcttac aaaatacatc
     1321 gacaactgga gtaaagacga cgatttctgt ctgcatatac tgaatccgct cttaaaaatg
     1381 tacccaaacc tgatcgcaca cacaaaaaaa tgggctatgt caaaaaatat gtggcttaga
     1441 agggcctcag cagtttgtat gatatcctcg gacaaatcaa tgtatgtatc ggaacattct
     1501 ttaaacgata tatttgaagt atcaataaag ctaatgggcg atcgagaaga cctggtacaa
     1561 aaaggctatg gctggttact aaaagcagct tcgatacgca agcagagaga agtgtttgat
     1621 tttattatag aaaataaact caaaatgagc agaacctcct taagatatgc tatagagaaa
     1681 atgcctccgg aactaagaaa aaaggctatg gagaaataag cattaaacga aaaatttttt
     1741 ttcggtaaaa tttacgaatt ttgctcaaga taagacacaa agaaatcttt caaaacttct
     1801 gggtgaactt ccatgttctt aaaataagag gggataaccc atacaggtct gacaagtaaa
     1861 ttaggatttt ctttcaaatg ctttatttcc acctcatcta aaagctcggg ctcgccgcta
     1921 acgtattcgc aaagatataa agattgctcg cactttctta aaggatcgta ggctgaaaaa
     1981 agaaatttaa caacctttgc ttgaatggag gtttcttcat aaagctcacg taaaacggtt
     2041 tgctctaaag tagcgtcggt ttcctcatag gttcctccgg gaattgttag atatacagca
     2101 ccatttttat actttagcaa cactaacacc atgccgtctt ttataatcag agctctagat
     2161 tttttagcag atttcatgcg aacataataa catactaggc aacagcttgg tttaacaaga
     2221 ttgattatta ctattttata atttcgttac ttaaatctac ttgtgttagc tatagaagca
     2281 ctttacttaa tatgctcggc ttttcggcga aaagaaatta cagcaaacca tctgcataag
     2341 ttggtggtgg ttgataagtt cggcgtaggt ttgtgtatta tttttagcgg ttttaggttt
     2401 tttggttgtt tatttttgag ggcgggctga aagcccgccc tcttcttatc
//
LOCUS       DAOSWN010000071         2428 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_34164_length_2428_cov_5.095660, whole genome shotgun sequence.
ACCESSION   DAOSWN010000071 DAOSWN010000000
VERSION     DAOSWN010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34164_length_2428_cov_5.095660"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..902)
                     /gene="uppS"
                     /locus_tag="PLF29_03320"
     CDS             complement(123..902)
                     /gene="uppS"
                     /locus_tag="PLF29_03320"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HXK52991.1"
                     /translation="MSQYRDIPVEKMPNHIAILPNGNRTWARQRGLSPMEGHNKGVEV
                     IASLARELRVIGVHTASVWGLSTENFANRDKNEVENLIRLLSYSLDKWGNEAHKDGAR
                     LIHLGRRDRLPKKVIDLIDKWQEKTLNNTKHTLNIAFDYGGHDELLRAMKKAFDDVTE
                     GKISFSDLEKTDGMYHDKYPYRFFKRYLDTGDQPYPYPDLIIRGAGELRLSGWMPWQS
                     VYSELYTENVLLPDFDEELIYRALKAYSTRDRKYGGSSDSA"
     gene            1054..1722
                     /locus_tag="PLF29_03325"
     CDS             1054..1722
                     /locus_tag="PLF29_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52992.1"
                     /translation="MDLLVISNFLVKYKYKLVLSSALGILLGSLVFITRPTYFNVSQT
                     YAILIQTEESPKPKIQLQADRLFDADSPQNEAEALTRSLAWIAQSSNFQSSIEETRIK
                     YNKQNPQKEISANTKMKVQNPAPHILLTTTVARDRESAFWYHKVLEQALQAQIFDLNP
                     TIEPDLKLIVLNEQPEIKEVKAALLYWTIVGTALGFGGGAFFLATREYVSMQQNTVKK
                     AKRN"
     gene            1920..>2428
                     /locus_tag="PLF29_03330"
     CDS             1920..>2428
                     /locus_tag="PLF29_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXK52993.1"
                     /translation="MKLALVVIPAYNEDSQIEPLLGQIKEITDIDIVVVNDGSTDKTL
                     EVCKNLNVKCLSHLINLGYGAAVRTGFAYAKKHPNYKYIVTMDGDGQHTVDQLKLLLE
                     ECKKNQLDLIVGRRVRKEQMPISRKAINALADLFFWALSGKYFVDTQSGLEAIRLNSL
                     YDLELNSVD"
BASE COUNT          756 a          518 c          461 g          693 t
ORIGIN      
        1 gatgtagttt gcccagtatg ttggtctttg caacgtaaag aaattacagc aagccgcctg
       61 cttaagcttg tggtggttga taaatagaag cactgtagca aagactgcaa ataacactta
      121 aactatgcgc tatcgctact tccgccgtac ttacgatctc gtgttgaata ggctttaaga
      181 gccctgtaaa taagctcttc atcaaaatcg ggcaacaata cgttttcggt gtaaagttca
      241 gagtaaacac tttgccaagg catccatccg cttaacctaa gctctccggc acctctaata
      301 ataagatcgg ggtaggggta tggttgatcg cctgtatcga gatagcgctt aaaaaatcga
      361 taagggtatt tgtcgtggta catgccatcg gttttttcta aatcactaaa actgattttt
      421 ccctccgtca catcatcaaa ggccttcttc atcgctctta aaagttcgtc gtgcccaccg
      481 tagtcaaaag ctatattcag ggtatgtttg gtattattta gtgttttttc ttgccacttg
      541 tcgataagat caattacttt ttttggcagc ctatctcttc gccccaagtg aattaatctt
      601 gcgccgtctt tatgggcctc gtttccccac ttgtctaaag agtaggacaa caaacgaatt
      661 agattctcaa cctcattctt atcacgattt gcaaaatttt ctgttgataa accccacaca
      721 gaggctgtat gaaccccgat gaccctgagc tctctagcca gggaggctat tacctctacg
      781 cctttgttgt gaccttccat aggagaaagt cctctttgcc ttgcccatgt tctattaccg
      841 ttaggtaaaa tggctatgtg gtttggcatt ttttcaactg gaatatctcg atactgactc
      901 atgttttaac tttttatttt actaataaaa tctactaaag atagatgcct ttcgtttgtt
      961 tgttttatgg caggcaaaac taaacatgct ttaacctatt taagatctac gagcttggta
     1021 attataccat taaatatgct gtataataca cttatggacc tattagttat ctcaaatttt
     1081 ctagtaaaat acaaatacaa gcttgttctt tcttcagccc ttggcatatt acttgggtca
     1141 ttggttttta ttactcgccc aacatacttc aacgtaagtc aaacctatgc catcttaatt
     1201 caaactgaag aaagcccaaa acctaaaatt caactacaag ccgaccgatt atttgatgcc
     1261 gattctcccc aaaatgaggc ggaggcttta acacgctccc tggcctggat agcgcaatcc
     1321 tcaaactttc agtcatccat agaggaaacg agaataaaat acaataaaca aaatccgcag
     1381 aaagaaattt ccgcaaacac aaagatgaaa gttcagaacc cggcaccgca catactacta
     1441 acaactaccg tagcgagaga tagagagtct gctttctggt accataaagt tttagagcag
     1501 gccctgcagg cacaaatttt tgaccttaat cccaccatag aacctgactt gaaacttatc
     1561 gttttaaacg aacagcccga gataaaggag gttaaagccg ccctacttta ctggacaatc
     1621 gttggaacgg cactaggttt tgggggcggg gctttttttc tagcaacaag ggaatacgta
     1681 agcatgcagc aaaatacagt aaaaaaagcg aaacggaact aatttcgcat caattttaaa
     1741 gcgagcttaa aacacataaa cacacactcc aacatatata gatcaaccac caccagctta
     1801 agcaggcggc ctgctgtaat ttctttacgt tgcaaagctc aacatgctgg ataaactaca
     1861 gctactattt gcgtaagtag gtttgagtaa cgaaattata aattgtgtat aatgctttca
     1921 tgaaactagc gctagtggtc attccggctt acaacgaaga ttcgcaaata gaacctttgc
     1981 tggggcaaat caaagaaatt actgatattg acatagtcgt tgtaaacgat ggcagcaccg
     2041 ataaaacact ggaagtatgc aaaaacctaa acgttaaatg tctatcccat ttaataaatt
     2101 tgggatatgg ggcggccgtt agaacgggct ttgcttacgc aaaaaaacac ccaaactaca
     2161 aatatattgt cactatggac ggagacggac aacataccgt tgatcaatta aaactcttat
     2221 tggaagagtg caaaaagaac cagctcgacc ttatagtcgg aagaagggta agaaaggagc
     2281 aaatgccaat aagtagaaag gctattaatg ctctagccga cctgtttttc tgggcattaa
     2341 gcggaaagta ctttgtagac acacagtcgg gacttgaagc aatacgtttg aattccttat
     2401 atgatcttga gctgaattcc gtagatta
//
LOCUS       DAOSWN010000072         2365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_35630_length_2365_cov_4.450649, whole genome shotgun sequence.
ACCESSION   DAOSWN010000072 DAOSWN010000000
VERSION     DAOSWN010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35630_length_2365_cov_4.450649"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="PLF29_03335"
     CDS             <1..698
                     /locus_tag="PLF29_03335"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HXK52994.1"
                     /translation="LNREESCGAHFREEYQTEDGEALRNDKKFAYVAAWEYKGDGIAY
                     IRVSRFGGSTNAEWSKAVEEITVQVPQLDSVVLDLRGNPGGYLQSAVYLAEEFLNKKV
                     VVIEQFPDGNEITLKADRVGKFEKLPKIIILLDGGSASASEILASALKDNLDTVTIVG
                     TTSFGKGTIQDVREFKEDGSALHVSIAKWLTPNKEWVHKKGIEPDVLVEVSDEDLANE
                     LDPQLDRALELAK"
     gene            743..1885
                     /locus_tag="PLF29_03340"
     CDS             743..1885
                     /locus_tag="PLF29_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXK52995.1"
                     /translation="MEQGFISRIIGNSKRFIKGFFWFGAFLSIVLAAFFYNRSAQKSD
                     HDLEVPARQKIILQNKDVEEESALIQMIDKVSPSVVSIVVKTTGFDIFNGSYSVEDGI
                     GTGFIVDEKGLIVTNDHVVDSVKSNYAVVTSDGTTYDVKRISKDPYADLAILEIDASG
                     LPVVELGDSSILRLGQTAVAIGNALGTYDNTVTSGVVSGIARQVVASLGFGDSKTYED
                     VIQTDAALNPGNSGGPLLNLEGQVIGINVATIWGAENIGFAIPVNTLKPLLEVYMSEG
                     RLIRPYLGVSYNMVSKNLAELRDLPEGAFILSVSPNSPAEEGGLRRGDIIIKIDDVNI
                     TESSTLSAVIANKRVGDKLTIRVVREDEELEISVVLAEFPDTSVFE"
     gene            complement(2006..>2365)
                     /locus_tag="PLF29_03345"
     CDS             complement(2006..>2365)
                     /locus_tag="PLF29_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HXK52996.1"
                     /translation="GQNVNKVSTSVRLTHVPTGVVVECQEERTQGKNRAKAMTMLESK
                     LFTSMQEQRVQKLADLRLSSIGTGERHEKIRTYNFPQSRVTDHRIKQSWHNLEAIMDG
                     DIKSMLTTLMTTEFDKA"
BASE COUNT          672 a          414 c          544 g          735 t
ORIGIN      
        1 ccctcaaccg ggaagaatcc tgcggggcgc atttccgcga ggaataccag accgaggacg
       61 gcgaggctct tcgcaacgat aaaaaattcg cgtacgtggc ggcctgggaa tacaagggtg
      121 acggcatcgc ctacattcgc gtaagtagat ttggcggatc gacaaatgcc gagtggagta
      181 aagcagtaga ggagatcacc gtgcaagttc ctcagcttga ttctgttgtt ttagatttgc
      241 gcggaaatcc gggcggttac ctccaatcag ccgtatattt ggctgaagaa ttcttaaata
      301 aaaaggttgt tgttattgag caattccccg atggcaatga aattacgctc aaggcggata
      361 gagttggtaa attcgagaag ctccccaaaa taatcatctt gcttgatggg ggaagtgctt
      421 ctgcttcaga aattttagct tcagccctaa aagataattt ggatactgtg actattgttg
      481 gtacaacttc ttttggaaag ggaacaatcc aagatgttcg agaatttaaa gaagatggat
      541 ctgctttgca tgttagtatt gctaagtggc tcacgcccaa taaagaatgg gttcacaaaa
      601 aaggcattga gccggacgtt ttagttgaag taagtgacga ggatctggca aatgagttag
      661 atcctcagct cgatagagct ttggagcttg caaaataaag gttatgtttt gcatatttta
      721 ttaataattc tataattttt atatggaaca aggttttatt tcaagaataa taggtaattc
      781 aaagaggttt ataaagggat ttttctggtt cggtgcattt ttatctattg ttttggcggc
      841 atttttttac aacaggtcgg cgcaaaaaag tgaccacgac ttagaagttc ccgctcgtca
      901 aaaaataatt ttacaaaata aagatgttga agaagagtcc gctctaatac aaatgataga
      961 taaggtctca ccttctgttg tttctatagt agttaaaact accggttttg acattttcaa
     1021 cggaagttac tctgtggaag atggtattgg tacgggtttt attgttgatg aaaagggatt
     1081 aattgttaca aacgatcatg tcgttgactc tgtcaagtcc aactatgcgg ttgtgacctc
     1141 tgatggtact acatatgatg taaaaagaat cagtaaagac ccttacgctg atttggcgat
     1201 attggaaata gatgcctccg gactcccggt tgttgagtta ggggattctt caatattaag
     1261 gcttggtcag acagctgtag caataggtaa tgcactaggg acttacgata acacagttac
     1321 ttccggagtg gtttcaggaa ttgccaggca agtggttgca tctttaggtt ttggtgacag
     1381 taaaacatac gaagacgtta ttcaaaccga tgcggcttta aaccccggta attcgggagg
     1441 gccactttta aacttggagg ggcaggtaat tggaattaat gtagctacaa tatggggagc
     1501 tgaaaatatc ggctttgcca tacctgtaaa tactttaaaa cctttacttg aagtttatat
     1561 gagcgagggc cgtcttatta ggccctattt aggagtttca tataacatgg taagtaaaaa
     1621 tcttgctgaa ttaagagatc ttcctgaggg cgcttttatc ttatctgtgt ctcccaattc
     1681 ccctgctgag gaaggtggct tacgaagagg agacataatt attaaaattg atgacgttaa
     1741 cattacggaa tcaagcactc taagcgcagt tatcgccaat aagagggtag gggacaaatt
     1801 aaccattcgg gtagttcgtg aagatgagga gcttgaaatt tcagtagttt tagctgagtt
     1861 tccggatacg tcagtttttg agtaaatcga acactagttt tttctaaatt tttttactgc
     1921 aatagaggcg caaacttcag tttttggatt tagataaaag tgggtagcaa taaagaagag
     1981 cctaagagct tagatccatt gtaattcaag ctttatcaaa ttcagtggtc attaatgttg
     2041 ttaacatgga tttgatgtcc ccatccataa tagcttcgag gttatgccag ctctgcttaa
     2101 tcctatgatc tgtaactctg ctttgtggga aattatatgt cctaattttt tcatgtcttt
     2161 cgcctgtccc aatagacgac agccttagat ctgccagttt ttgcactctt tgttcttgca
     2221 tagaagtaaa cagcttagat tcaagcattg tcatggcttt agccctgttc ttgccttgtg
     2281 tgcgttcttc ttgacactct actaccactc ctgttggaac gtgagttagt cttactgaag
     2341 tactgacttt gtttacgttt tgacc
//
LOCUS       DAOSWN010000073         2363 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_35678_length_2363_cov_4.054159, whole genome shotgun sequence.
ACCESSION   DAOSWN010000073 DAOSWN010000000
VERSION     DAOSWN010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35678_length_2363_cov_4.054159"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..968
                     /locus_tag="PLF29_03350"
     CDS             210..968
                     /locus_tag="PLF29_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52997.1"
                     /translation="MKLSPKAQASIDKVVNQFKTGDLSPISKVARIRLDVSAPARKWS
                     LSNKVLAFMQAEELDCRGFVQWQQLGRQVKRGSKAVYIFRPHTITKTTEDDEKEAVCI
                     GFSTVPVFAASCTEGDGILPGYAPAELPPLTEVAKKFGVQVDYVPVTPDKLGDCKIDG
                     SKIRLGSHDTRVFFHELTHAIHASIDGGLKGGQQTDQETIAEFTTAVLMDFYGFGDNT
                     GNAWRYISHYSEDPIIAITKALGTVEKVLGVLLN"
     gene            complement(957..1769)
                     /locus_tag="PLF29_03355"
     CDS             complement(957..1769)
                     /locus_tag="PLF29_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52998.1"
                     /translation="MLYYLPRESSAHSEQAMETTKLTPKQLEILLLLYRYRFLDRTHI
                     QRFLHHKDPKRINTWLKDLTDKNIIGRKYSRKLKENTKPAIYHLATKSRKILLERNDT
                     NEKLLKRVYREKTRSQRLIDHCSLVADMHFLLLSKLNKNEKLHFFTQTDLVNHYYLPY
                     KRPDAYIAVEGEKETKRYFLEIIDEGTPRFLLRSRIASLIEYFDEKTWYKKTNNTDPA
                     LLFVCPNLTLKTFLYRHISQVLEEEAEDELQFYLTSKEQIDYSDGNIWELVE"
     gene            1924..2247
                     /locus_tag="PLF29_03360"
     CDS             1924..2247
                     /locus_tag="PLF29_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK52999.1"
                     /translation="MKGNRKLNYAITYGVLFGLVDASLMLPLEFENKALAMTGAFLQR
                     FAIGFLIPYINLPLSGTLKGIFSSFLISIPTAVITGSYIPIITTGVIGGAIIGWHSEK
                     IKYGK"
BASE COUNT          758 a          395 c          479 g          731 t
ORIGIN      
        1 gaaattagca gtaaaggtaa acttggtcta atagcttcaa acatagataa agtatagcaa
       61 taaaaggggt cttacccctc attttgcaag ggcttagcaa gtcttgactt acagtcacga
      121 cccttgcaaa actcaccccg cttggaattt cttcatatat ggaggcaggc acgccagcct
      181 ccaataaaaa atttgaagaa aggaaaagca tgaaactctc acctaaagca caagcaagca
      241 tagacaaagt agtaaatcag tttaaaacgg gagatctatc gcccattagc aaagtagcca
      301 gaattaggct tgatgtgtct gcaccagcta gaaagtggag cctatcaaat aaagtactag
      361 cctttatgca ggctgaagaa ttggattgta gaggttttgt gcagtggcag cagttaggta
      421 gacaagttaa aagaggaagc aaggcggtat atatatttcg acctcacaca attacaaaaa
      481 caactgaaga tgatgaaaaa gaagcagttt gtattggatt ttcaacggtt ccagtctttg
      541 cagcatcgtg cacagaagga gacggaattt tgccagggta tgcacctgca gaacttcctc
      601 cactaactga agttgcaaaa aagtttggag tacaggttga ttatgtacca gttactccgg
      661 ataaattggg agactgcaaa atagatggct caaagattcg cctaggtagt catgatacta
      721 gagtattttt ccacgagtta acacatgcca tacacgccag tattgatgga ggtttaaaag
      781 gcggacaaca aaccgaccag gaaacaatag cagaatttac cacagcagta ttaatggatt
      841 tttatggctt tggagacaat accggcaatg cttggagata tatcagccac tattcagagg
      901 atccaataat tgccatcaca aaagccttag gcacagttga aaaagttctt ggggtattac
      961 tcaactagtt cccaaatatt gccatcacta tagtctattt gttctttgct tgttaaatag
     1021 aactgtagtt cgtcctcggc ttcttcttcc aatacttggg atatatgccg atataaaaaa
     1081 gttttgagcg ttaaatttgg acagacaaat aacaatgcag gatctgtgtt gttggttttt
     1141 ttgtaccatg tcttttcatc gaaatattca ataagtgatg caatacgact tcttaacaaa
     1201 aatctgggcg ttccctcatc aattatttcc aaaaaatatc ttttagtttc tttttcacct
     1261 tcaacagcta tatatgcatc tggtcttttg tagggcaggt aataatggtt tactaaatca
     1321 gtttgagtaa aaaagtgaag tttttcattt ttgttaagtt tacttaaaag caaaaagtgc
     1381 atatctgcta caagtgagca atgatctatg agcctttggg atctagtttt ttctctgtaa
     1441 acacgcttga ggagcttttc attggtatcg tttctttcaa gtaatatttt tctagacttg
     1501 gtagcaaggt gatatatagc aggtttggtg ttctctttta gtttgcgtga gtattttctt
     1561 ccaatgatgt ttttatcagt gaggtctttt agccaggtat taattcgttt agggtctttg
     1621 tgatgtaaga atcgttgtat gtgggttcta tcaaggaagc gatatcgata taggagtagg
     1681 agtatttcca gttgtttagg tgtaagtttt gtagtttcca tagcttgttc actatgggca
     1741 gaactttctc ttggcaagta gtataacatt ccctcccttt atacttaaag gaataggggt
     1801 aaaagaaaaa tattaaaatg cctatatatt gggaaaaact aaaatacaat tgtcctaaca
     1861 ggctagggca aaaattccgt caggtgcaag atgctttcca atttaatata ataaatttaa
     1921 cttatgaaag gaaacagaaa actaaattac gcgatcactt atggtgtgtt attcgggttg
     1981 gtagatgcct cattaatgct gcctctagaa ttcgagaaca aagcattggc aatgacagga
     2041 gcatttcttc agcgctttgc tatcggattt cttattccgt atataaacct tccactttca
     2101 ggtacgttaa aaggcatttt cagtagtttt ttaataagta tacctacagc agtgataaca
     2161 ggatcctaca taccgattat tacaactggc gtgatcggtg gagcaattat tggttggcat
     2221 tcagagaaaa taaaatatgg gaagtaattt aattgacgaa aaatcccaag aagtaaaagc
     2281 aatttatgag gctattttga atagtcttaa aagctttgga agatttgaaa ttgagctcaa
     2341 aaaaacttct atacatatcg tta
//
LOCUS       DAOSWN010000074         2330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_36507_length_2330_cov_4.629451, whole genome shotgun sequence.
ACCESSION   DAOSWN010000074 DAOSWN010000000
VERSION     DAOSWN010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36507_length_2330_cov_4.629451"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..1081)
                     /locus_tag="PLF29_03365"
     CDS             complement(86..1081)
                     /locus_tag="PLF29_03365"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HXK53000.1"
                     /translation="MKLLITREIPDAGIKILEQYPSIEIDYRKGPPLSEEALLKAVVG
                     VDAILPVIPDKITQKIIEAAGQNLKIIAHYAVGYDNIDIETATKNKVYVSNTPGDLTE
                     SVAEFTLGLIYALSKNIVTADKFVRNGNYQFWDPMLFLGPTLRNKVLGIIGMGRIGQH
                     LARLAHGGLKMKILYSDPKEHIDIEKECDAKKVELEELLTNSDFVSLNCPLIESTRHL
                     INQKELELMKPTAYLINTARGPIVNEAALYEALKNNTIEGAALDVFEDEPSLYNGLTS
                     LNNIILTPHIASATREARIQMARMAAQNIIDVLIKGTAPTNLVNMELADKIGKIA"
     gene            complement(1140..2096)
                     /locus_tag="PLF29_03370"
     CDS             complement(1140..2096)
                     /locus_tag="PLF29_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HXK53001.1"
                     /translation="MAYDVLVIGDSSIDQYLELESVEIHEDLKMKQPEICFVHGSKVP
                     VKTFASTLAGNACHVAVGLQRLNINTAIYTELGEDQNGSRFIEKYKSYGINTHLCRQN
                     KGVDTNVHTIIWGKGDRTIFSYHYPYKYEVEFTKLPQISWVFYTSLADGFETFQADLV
                     KYLNANPSIGLAFNPGTYHMRAGTGKLLNVLKRTDILFVNREEAYAIIGLSTGLSAAQ
                     TELHEKLQKLGPKKTVITNGKHGSSASDGESYEEMPIIKVAEPIVDKTGAGDAFAAAF
                     MAAIYYKKSLKEALLWGTKNSASIVKCVGCIDGLLTKKEITS"
     gene            complement(2100..>2330)
                     /locus_tag="PLF29_03375"
     CDS             complement(2100..>2330)
                     /locus_tag="PLF29_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK53002.1"
                     /translation="IIISMFNVFNNLTTEQGIMHTAEFFSLLFVFSITYHAIHNIKGT
                     IKPPTKALISMFFGTVAYGIAKIIIGLFFGTY"
BASE COUNT          580 a          451 c          476 g          823 t
ORIGIN      
        1 ggccatatgt tcccgtatta ggcatataac tctttatact atttttttgg atatttggca
       61 aaatccttta attttttaca taaattcaag ctatcttgcc gattttgtct gcgagttcca
      121 tgttaactag gttggtgggg gcagttcctt ttattagtac gtctattatg ttttgtgcag
      181 ccattctggc catctgaatt cttgcttctc ttgttgcgct tgctatgtgt ggggttagaa
      241 ttatgttatt gaggctggtt agtccgttat aaagacttgg ttcgtcttca aagacatcta
      301 gagccgcacc ttctattgta ttatttttta gagcctcgta taaggcggcc tcatttacta
      361 tcggtcctct cgcagtattt attaaatagg cggtgggttt catgagctca agctccttct
      421 gatttattaa gtggcgcgtg ctttctataa gggggcagtt taatgaaaca aaatcagagt
      481 tcgttaataa ttcctcaagc tcaacttttt ttgcgtcaca ttctttttct atatcaatgt
      541 gttcttttgg atcggagtac aagatcttca tttttagacc gccgtgggcg agcctcgcca
      601 ggtgttggcc gattcttccc atgcctataa tccccaaaac cttatttctt aatgtagggc
      661 ctaggaaaag cattgggtcc cagaattggt agttaccgtt tcgtacaaat ttatcggccg
      721 ttacaatgtt tttgcttaga gcatagatta gccctagtgt aaactcggcc actgattcag
      781 ttagatcgcc cggagtgttt gatacataaa ctttgttttt tgttgcagtt tcaatgtcta
      841 tgttgtcata acccacggca tagtgcgcga ttatttttaa gttttgtccg gccgcctcaa
      901 taattttttg tgttatctta tcgggaatta ctggtagtat tgcgtcgacc cctacaaccg
      961 ccttaagcaa cgcctcttct gaaagagggg ggcctttcct gtagtctatc tctatagaag
     1021 ggtattgttc taaaatttta attccggcat cggggatttc tcttgtgatg agcagtttca
     1081 tactttaagt gtagcattat taattttttt cataaatatt ctatttgtga gggcgctgtt
     1141 tatgaggtta tctctttttt agtcaggagg ccgtcgatac atcctacaca ttttactatg
     1201 ctggcagaat ttttagttcc ccaaagtaaa gcttccttaa gacttttttt atagtatatc
     1261 gccgccataa aagccgccgc aaaggcgtcc ccggcacctg ttttgtctac aataggttct
     1321 gcaaccttaa ttatgggcat ttcctcgtaa gattctccat cagaagcgct ggagccgtgt
     1381 tttccgttag taataactgt ttttttgggc cccagttttt gcaatttttc gtgtagttct
     1441 gtttgtgccg ctgaaagccc ggttgagaga ccgattattg catacgcttc ctctctattt
     1501 acaaacaata tatctgtgcg ttttaagacg tttagcaatt ttcctgttcc tgcgcgcatg
     1561 tgatacgtgc cgggattaaa ggcaagtcct atggaagggt ttgcgtttag gtatttcacc
     1621 aagtcagctt gaaatgtctc aaacccgtca gctaaagatg tataaaaaac ccaagatatt
     1681 tgtggcaatt ttgtaaattc gacctcgtat ttatatgggt agtggtatga aaatatcgtg
     1741 cggtcgcctt tgccccaaat aatcgtatgc acgttagtgt ctaccccttt gttttgtctg
     1801 caaaggtgtg tgtttatacc gtaacttttg tatttttcta tgaacctaga accgttttga
     1861 tcctcgccaa gttcagtata aatggcggta ttaatattta gcctttggag acctacggca
     1921 acgtggcatg cgtttcctgc aagagtagag gcaaaagttt tgaccggaac tttgctacca
     1981 tgaacaaaac atatttcagg ctgtttcatc ttcagatctt catgtatttc aactgattca
     2041 agttcaagat attgatcgat agagctgtct ccgattacta atacatcgta agccatagat
     2101 taatatgttc caaaaaaaag cccgataata atttttgcaa ttccgtaggc aactgtacca
     2161 aaaaacatag agatcagtgc tttagtggga ggctttattg tgccttttat attatgtatt
     2221 gcgtggtagg ttatcgaaaa aacaaacaaa agtgaaaaaa attccgcagt atgcattatg
     2281 ccttgctctg tggttaggtt attgaagaca ttaaacattg agataataat
//
LOCUS       DAOSWN010000075         2287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_37611_length_2287_cov_6.886649, whole genome shotgun sequence.
ACCESSION   DAOSWN010000075 DAOSWN010000000
VERSION     DAOSWN010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37611_length_2287_cov_6.886649"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="PLF29_03380"
     CDS             complement(<1..545)
                     /locus_tag="PLF29_03380"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HXK53003.1"
                     /translation="MVNKSNGVEKSRIEGATITDIISHIREAEKNGSKFITDQLTERL
                     KGRSNRDTAKKIWEFIRSNIQYLEDGEHQKIKSPAKLWSDRVGDCKSMTVFAASVLQK
                     MCIPYDLIVDFYYKDRPNSGHIYVKLKDGTVLDPVNPKFNAVDKAWKSYRIPGHNICK
                     PGNISGKTKTKKMSTISSYIEG"
     gene            complement(539..967)
                     /locus_tag="PLF29_03385"
     CDS             complement(539..967)
                     /locus_tag="PLF29_03385"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HXK53004.1"
                     /translation="MKIEQLALIAIGVYLFLKSQADKISYSIMRNYVQEWGFDGVTIR
                     TDISIKNASNLPINIDGFQGKVMYRGAEIAQVGLINSVNISAGNEGKITVGAKILYGP
                     ATAVIIESIRTGRIESGVRLVGTIYADRLKIPVSTNYNLW"
     gene            complement(1120..1959)
                     /locus_tag="PLF29_03390"
     CDS             complement(1120..1959)
                     /locus_tag="PLF29_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK53005.1"
                     /translation="MRKTKLTYSNEAELEFICKGVEQSLMSGKYKFTKTRTTGTAKYQ
                     TKNEQILILPGNNELAGANARFVGSIKKHFKASQLPERISDERPFYEYFVPISTYTEL
                     KNICEVDINGAYWESAYELGYITKDLYKSAETVSKKARLIALGVLGKKTAVSVFEPPY
                     SEIETTEHYERERVFWDNIVWNLGETIRDVTSRYKPHIFGIWFDAIFCTKSSANNIRR
                     YLQRRGYETKIKQTRSYVITPRDGIAEGAKIERIFMDGTRKEMDVTYTRKKTMDQFLE
                     SIK"
     gene            complement(1970..>2287)
                     /locus_tag="PLF29_03395"
     CDS             complement(1970..>2287)
                     /locus_tag="PLF29_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK53006.1"
                     /translation="PMKGGDSFGPKRVVEATENKAFFEGKQLTKVIRINAPDELGGTR
                     IATTMQEVEAIRGELMRAYGANVAANNPKGSYGLIGADYSVEYHSTGNNLLAVIDIDM
                     FLY"
BASE COUNT          535 a          530 c          400 g          822 t
ORIGIN      
        1 ccttcaatgt agctggatat ggtagacatt ttttttgttt tggttttacc tgaaatgttt
       61 ccaggtttgc aaatgttgtg tcctggtatt cggtagcttt tccaggcctt atcgactgca
      121 ttaaatttcg gattgactgg atctaatacg gtcccgtctt tcaatttaac atagatatgt
      181 ccagaatttg gccggtcttt atagtagaaa tcaacaataa ggtcgtaagg aatgcacatt
      241 ttttgaagta ctgaagcggc aaaaacggtc atgcttttac agtcccctac cctatcggac
      301 caaagtttag cgggagactt tattttttga tgctctccat cttccaggta ttgaatattt
      361 gaccttatga actcccatat tttttttgcc gtgtcacggt ttgaccttcc ttttaaccgt
      421 tcggttagct gatcggtaat aaatttagat ccgttctttt cagcctccct gatatgggaa
      481 attatgtccg ttattgttgc cccctctatg cggctttttt ccactccgtt gctcttgttt
      541 accataggtt ataatttgtt gatacgggaa tctttagccg atctgcgtag attgtaccaa
      601 ccaaacgaac gcccgattct attctacctg ttctgattga ctctattatt accgctgtag
      661 ccggaccgta aagaatcttt gcccctactg ttattttacc ctcgtttccc gccgatatgt
      721 tgacgctatt tattagccct acttgcgcta tctctgcccc tctgtacatt accttgccct
      781 gaaatccatc tatgtttata ggtaggttgc ttgcgttttt tattgatatg tcagtcctta
      841 ttgttacccc atcaaagccc cactcttgta cgtagtttct cataatagaa taacttattt
      901 tatctgcctg gcttttcaaa aacagatata cgcctattgc tatcaatgcc aattgttcta
      961 tcttcattgt gttaaaattt ggggagacaa aagaaagcct ccccataaat aaccccaaaa
     1021 aaccaataag ttatgcagtt gcaagttagt aatttttcaa taaaaaagcc ggtcattttt
     1081 agaccggcct aaaaataaaa ttataatccc atgaaaatct tattttatgg actctaaaaa
     1141 ctgatccatt gtcttttttc ttgtgtacgt tacgtccatc tcttttctag ttccatccat
     1201 gaagatacgc tcaattttcg ccccttcagc tatcccgtcc ctgggtgtta ttacgtaaga
     1261 tctggtttgt ttaatcttgg tttcgtatcc cctacgctgc aagtatcttc gtatgttgtt
     1321 tgcacttgat ttggtgcaaa atatggcgtc aaaccaaatg ccaaaaatat gaggcttata
     1381 tctggacgtt acgtctctta ttgtctcccc gagattccag acaatgttat cccaaaaaac
     1441 acgctccctt tcgtagtgtt cagttgtttc aatttctgaa tatggaggtt caaaaactga
     1501 aacggccgtt ttttttccta acactccaag ggctattaat ctagcttttt tgcttacggt
     1561 ttcagctgac ttatacagat cctttgttat gtatcctagt tcgtatgcac tctcccagta
     1621 tgccccattt atatctactt cgcaaatgtt cttaagttcc gtataggtac tgatcggtac
     1681 aaagtactcg tagaatggac gctcgtcgct tattcgttcc ggcaactgcg atgctttaaa
     1741 gtgcttcttt atactaccta caaaacgggc gtttgcccct gccagttcat tattcccagg
     1801 cagaatcagt atttgctcat tctttgtttg gtactttgcc gttccggttg tcctggtctt
     1861 ggtaaactta tacttgccac tcattagaga ttgttccacc cctttgcaga taaattcaag
     1921 ttctgcctca ttactgtatg ttagctttgt ttttctcatt ggtttggttt tagtataaaa
     1981 acatatctat gtcaatgact gccagtaaat tgtttccagt gctgtgatac tcaacggaat
     2041 aatcggcccc gatcagccca taactcccct ttgggttgtt ggcggctaca ttggctccat
     2101 atgctctcat tagttcccct ctaatggctt ccacctcttg cattgtcgtg gctatccttg
     2161 ttccacctaa ttcatcggga gcgtttattc ttatcacctt tgttaactgc ttgccttcaa
     2221 agaaggcctt gttctcggtt gcctctacta ccctcttagg accaaaacta tccccgccct
     2281 tcattgg
//
LOCUS       DAOSWN010000076         2273 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_37978_length_2273_cov_3.936429, whole genome shotgun sequence.
ACCESSION   DAOSWN010000076 DAOSWN010000000
VERSION     DAOSWN010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37978_length_2273_cov_3.936429"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="PLF29_03400"
     CDS             <1..664
                     /locus_tag="PLF29_03400"
                     /inference="COORDINATES: protein motif:HMM:NF025124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK53007.1"
                     /translation="ILYRKTISNVSLLAQFDSTVNIFEQYYLFEEIDGYNRIKKELIP
                     REAFRESLANAIVHRVWDTNSYIQIAMYEDKIEINSPGGLPTGIAEDEYLYGNISVLR
                     NPIVAGVFNRLNIIEKFGTGIARINKEYTHSITKPAFEVSENRIRIILPVCENDLIDL
                     SEDENKIYQLLKEEIELSRKEIDLKTGFNKAKTLRILNRLVDKNTIQKIGSGATLTYK
                     LK"
     gene            847..1035
                     /locus_tag="PLF29_03405"
     CDS             847..1035
                     /locus_tag="PLF29_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HXK53008.1"
                     /translation="MTSKELIRKLKKIGWKEEDSTKGKGSHVALVKGDKELIIPFHTN
                     KDLKPGTLHRLLKDAGLK"
     gene            1141..1545
                     /locus_tag="PLF29_03410"
     CDS             1141..1545
                     /locus_tag="PLF29_03410"
                     /inference="COORDINATES: protein motif:HMM:NF027249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HXK53009.1"
                     /translation="MKKKKRVAYTAIFEWGEDGSYDVSFPALAGCYSSGDSFMEAYEC
                     SKEAIGLYLTTISKRGKAFPDEKALDVKNLELSENERLVIVVVEDINKYRPQKAVNKT
                     VTLPYWLNEIAEKEHVNFSGVLQEALKEKLEV"
     gene            1810..2091
                     /locus_tag="PLF29_03415"
     CDS             1810..2091
                     /locus_tag="PLF29_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK53010.1"
                     /translation="MSRALSRGDEIPAPSSLRDIKLEKNEATHVIDIFMPPMRERIKN
                     RFVKKTLSLPAWLADKADEAGVNFSKIFQNALMEYLDVSQQDKHQNIRK"
BASE COUNT          794 a          371 c          477 g          631 t
ORIGIN      
        1 gattttgtat agaaaaacca taagcaatgt atcgctatta gcgcaattcg atagtaccgt
       61 aaatattttt gagcaatact atctattcga agagatagac ggctataata gaataaaaaa
      121 agagttgatt cctagagaag cttttagaga gtccttggcg aatgctattg tccatagagt
      181 ttgggatacc aattcttata tacaaatagc aatgtatgaa gacaaaatag aaataaactc
      241 acctgggggg ctcccaacag gaatagctga agacgaatac ttatatggta atatttctgt
      301 actcagaaac ccaatcgtcg caggggtgtt taatcgatta aatattattg aaaagtttgg
      361 tacaggaata gcaagaatta ataaagagta tactcatagc attactaagc ctgcctttga
      421 ggtaagtgaa aatagaatta gaatcatact accagtttgc gaaaatgatc ttatagactt
      481 atctgaagat gaaaataaga tataccagtt attaaaagaa gagatagaac tttcacgaaa
      541 agaaatcgat ttaaaaactg gatttaataa ggcgaagacc ctgaggatac tgaataggct
      601 tgtagataaa aatactatac agaaaatagg tagtggagca acattaacct ataagttaaa
      661 gtagggcttg ctgaataact aagagcactt aataataatc tccgagggtc cacctcaaac
      721 cgtccccgct gacttattgt tgcttttttg ctttatatta tcacaattct acccgtgtta
      781 catttgacaa cactggtaac aagtgttata atcgataaac ctggagagga ggcattacca
      841 aacacgatga cttcgaagga actaatccga aaattaaaaa aaataggatg gaaagaggaa
      901 gattctacga agggaaaagg cagtcatgta gcgttagtta agggggataa ggaattaatt
      961 atccctttcc atacaaataa agacttaaaa ccaggaacat tgcacaggct tttaaaagat
     1021 gcaggattaa agtaatccag ttggggattc caatccccca ccttcactct tgaaggattt
     1081 gttgttagtt gataaactaa ataaaataga ggaatttgaa aggaacggag gttttgtggg
     1141 atgaaaaaaa agaaaagagt agcttatact gctattttcg aatggggtga agacggtagt
     1201 tatgatgtga gttttcctgc attagcaggg tgctattcat ctggagattc ctttatggaa
     1261 gcgtatgagt gttctaaaga agcaatagga ttgtatttaa cgactatctc aaaaagagga
     1321 aaagcatttc ctgacgaaaa agctctggat gtgaaaaatc tggaattatc tgaaaacgaa
     1381 cgtcttgtga ttgtggtggt tgaggatatt aataaatata gaccacagaa ggctgtaaac
     1441 aaaactgtga cattacccta ttggttaaat gaaatcgctg aaaaagaaca cgttaacttc
     1501 tcaggtgttc tccaggaggc tttaaaagaa aaactggaag tatagttcat aggcgtaatc
     1561 aattacctgt ataagcgtgg atgcgtttat gcaggttttg ttttatacct ctacccactc
     1621 aaataattaa gatatggttc tctctcatgt aatatgagtg gttagcaaga atgaaactac
     1681 caaactatct ccgttgctct gtgtattttt taaaattaat gtgttgacaa tgacaataca
     1741 gcgtgatata aagtcgaagg ccctcacaga taagccgtct ctcgaaatca gcagaaagaa
     1801 ccgtctccat tgtcacgtgc gttgtcacgt ggagatgaga taccagcccc atcatcctta
     1861 agagacatta agcttgaaaa gaatgaagcc actcatgtta ttgatatatt tatgccacct
     1921 atgcgagaaa gaatcaaaaa tcgatttgta aaaaaaaccc tttcactgcc tgcgtggttg
     1981 gcggataaag cggatgaagc tggtgtcaat ttttcgaaaa ttttccagaa tgccctgatg
     2041 gagtatttgg atgtaagtca acaggataaa caccaaaata ttagaaaatg aagacaaggg
     2101 aatttctgaa tgccttttta taagaatcaa tcagtttaag ttcttgttta ctccatttgg
     2161 ccctagattg taaacactaa tgggtccacc tcaaaccgtc cactttgtct tacgaaaaac
     2221 cttgcagatg cgaattcccc ccggtggggg gggtgccggc cctggcgggg ggg
//
LOCUS       DAOSWN010000077         2200 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_39987_length_2200_cov_3.629837, whole genome shotgun sequence.
ACCESSION   DAOSWN010000077 DAOSWN010000000
VERSION     DAOSWN010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39987_length_2200_cov_3.629837"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="PLF29_03420"
     CDS             <1..1035
                     /locus_tag="PLF29_03420"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HXK53011.1"
                     /translation="GSSLNPWNTSKVPGGSSGGSAIAVAADLCVFATGSETAGSIRGP
                     ASWCGVTGLKPTYGRVSRYGVIAMASSTDSPGVLTKDVKDAAFLLNVLAGKDPYDATT
                     SPVTPDNYLKSVDTESDVSEDLQGVTIGVPVEYMDAIQDKFYRARIEEALSVFAKLGA
                     KIKEISLLDPKYSIAIYTILQRSEVSSNLGRFSGVRYGEKREEFNFENKKRILLGTYA
                     LSSGYYDQYYAKAQKARTLLVEDFKNAFKEVDLIFGPTMPGVALSVGESKNYSMFGEL
                     IDLLQEPSSIAGLPAISLPAGMQEGLPVGVQIIANYFEENLMLRVSSAFQKKTEFHKF
                     KPSAQSYEHI"
     gene            1022..1408
                     /locus_tag="PLF29_03425"
     CDS             1022..1408
                     /locus_tag="PLF29_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK53012.1"
                     /translation="MSIFNIFRKLFYTRKFVSSETCVLIKRDWQNISSMLKGGSPSQM
                     RQALISADKTMDAALKDLVSGSGMGERLKYAKNLFSPDTYDKIWKAHKVRNNLVHEAG
                     YEPTYFVLKSSIEDLKRGLIELKVNL"
     gene            1410..>2200
                     /gene="gatB"
                     /locus_tag="PLF29_03430"
     CDS             1410..>2200
                     /gene="gatB"
                     /locus_tag="PLF29_03430"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HXK53013.1"
                     /translation="MTKDFRLILGLEIHLHVKTAKKMFCNCSADIYSAEPNTHVCPTC
                     LGLPGALPVPNKHAVELTQLLGIALGCTLNLDSRFDRKHYFYHDLPKGYQLSQYDQPL
                     CVGGSLKLRNGKTIELERIHLEEDTAKSFHSKDDTLIDFNKSGMPLIEVVTKPVITSA
                     EDAAEFGRMLRDLARFLNISDADMEKGQMRVEPNISVRTVEMERANELPEYKVEVKNI
                     NSFKAVEKAAEYEIARHIELLEAGRGDEIVQETRGWDENKQITFS"
BASE COUNT          688 a          396 c          504 g          612 t
ORIGIN      
        1 ggctcttcgt tgaatccttg gaatacgtca aaagttcccg gaggctcctc gggaggttcg
       61 gctatagccg tggcagcaga tttgtgcgtt tttgctaccg gatcagaaac tgcgggaagt
      121 attagagggc ctgcgtcttg gtgcggtgtt accggattaa agccgacata cggacgcgta
      181 agtcgttatg gtgttattgc tatggcttct tcaacagaca gcccgggtgt tctgactaaa
      241 gatgtcaaag acgctgcgtt tttattaaat gtacttgccg ggaaagatcc ttatgatgca
      301 acaacttccc cagttacccc agataactat ttaaaatctg ttgacacaga aagtgacgta
      361 tccgaagatt tgcaaggtgt aacaataggt gtcccggttg aatacatgga tgctattcag
      421 gacaagtttt acagagcgag aatagaggaa gctctgtctg tctttgccaa gctcggcgca
      481 aagattaagg agattagctt gctggatccg aagtattcta ttgcaattta caccatttta
      541 cagcgatccg aggtatcttc taatttaggt cgtttttcag gtgtcaggta tggtgaaaaa
      601 agagaagaat ttaattttga aaataaaaaa agaatattac ttggtactta cgccttaagc
      661 tcagggtatt atgatcagta ttatgcaaaa gctcagaaag ccagaactct gctggttgaa
      721 gattttaaaa acgccttcaa agaagttgat ctcatatttg gcccgactat gcccggtgtt
      781 gcactttctg tcggagaatc taaaaattat tccatgttcg gggaacttat tgatttgcta
      841 caagagccca gttcaattgc aggattgcct gcaatctcac tcccggcggg aatgcaggag
      901 ggtttgcccg taggcgtgca gattattgct aattactttg aagaaaacct tatgctgaga
      961 gtaagtagtg cttttcagaa aaagaccgag tttcataaat ttaaaccaag cgcacagtcc
     1021 tatgagcata tttaacattt ttagaaagct tttttacaca agaaagttcg tgagttcaga
     1081 aacatgcgtt ttgattaagc gagattggca gaatattagc tcgatgctca agggtggttc
     1141 tccgagtcaa atgaggcaag cactaataag cgccgacaaa actatggacg cggcccttaa
     1201 agatctagtt tccggctcgg gtatgggaga aaggttaaaa tacgcaaaaa atttattttc
     1261 ccccgataca tacgataaaa tatggaaagc acacaaggta cggaacaatt tagtgcatga
     1321 ggccggctac gaaccaacct attttgtttt aaagagctct atcgaagatc tgaaaagagg
     1381 acttattgaa ttaaaggtta atttataaaa tgacaaaaga ttttagatta attttaggtt
     1441 tagaaataca cttgcatgta aaaacggcta aaaaaatgtt ttgtaattgc agcgcagata
     1501 tatacagcgc agagccaaat acgcatgttt gcccaacatg cttaggtttg cccggcgccc
     1561 tgccggttcc aaataaacat gctgtagaac ttacgcaact cttaggtatt gcattaggat
     1621 gtacactaaa tcttgattca cgcttcgacc gtaagcacta tttttatcat gatcttccaa
     1681 agggctacca gcttagtcaa tacgatcagc ctttatgtgt tggcggatcg ttgaaactca
     1741 ggaacggcaa aaccattgaa cttgaaagaa ttcatttgga agaagataca gctaaatctt
     1801 ttcattcaaa agatgatact ttgattgatt ttaataagtc cggaatgccc cttattgagg
     1861 ttgtaactaa acctgtaatc acctcggcag aggatgctgc agaattcgga cgtatgttga
     1921 gagacttagc acgctttcta aacattagtg atgctgacat ggagaagggg cagatgagag
     1981 tagagccgaa tatttctgtt cgaacggttg aaatggagag agcaaacgag ttgccagaat
     2041 ataaagtaga agtgaaaaat ataaactctt ttaaagcggt agaaaaagcc gcagagtatg
     2101 aaatcgcaag acacatagag cttttagaag ctggaagagg ggatgagata gttcaagaaa
     2161 ctcgtggttg ggatgaaaat aagcaaatta ctttttctca
//
LOCUS       DAOSWN010000078         2172 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_40791_length_2172_cov_4.144072, whole genome shotgun sequence.
ACCESSION   DAOSWN010000078 DAOSWN010000000
VERSION     DAOSWN010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40791_length_2172_cov_4.144072"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..1700)
                     /locus_tag="PLF29_03435"
     CDS             complement(219..1700)
                     /locus_tag="PLF29_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXK53014.1"
                     /translation="MLPVVRSHCDYQSFVAEQLKTHYTGGILCIQSPDWNLIEKFWLT
                     DLSKTAFILRDTFSKQGPRPRDPADMLRSILLMVQTGVSSVTQWVTLLRRVPLYAILS
                     GFIPNKTPGVGTFYDFFARLWASDSPNFSCKKKRKTKKIKKGARKGEKAPTTSPNKVA
                     NLVKGLERRSAVFKFLPFDRLCALFKEIFVFKSAQLGLLGDIKNLCLAGDGTPVRTSA
                     YPRSKRTCDCREKGIFDCECPRIYSQPDCNIGWDSSRECYFHGYHLYVYTAAESFYDL
                     PVYPRLYKASRHDSISMLLSAEELQLRYPDWVISRVLLDAAHDAMPIYHYFNNRDILA
                     FIDLNKRRSGQTKYKDDFSLSESGIPICNKGLEMKDNGYDYTRGRRKYRCPLIKKGIV
                     ACDSPCSTSSYGRCVYTYTKENIRLFPPIARNSNEWKNAYKRRTSVERSNKREKVDYM
                     LEAARHRSSKMWNIRIYAIMMCQHMDAWFKESDLTLQSTLLTA"
BASE COUNT          734 a          428 c          478 g          532 t
ORIGIN      
        1 tatagaaata aattattggt attataatag gctttcggaa ttaaccgagg caaaaaggaa
       61 gcagcaagga aaaaagggac taaattccat ggaaaataga aaaagaaacc cggcaggcaa
      121 atgaggattt aaaaaagagc atagaaaagc aaaggtcgaa cctggtaaat tttttggctc
      181 caccgaaaag gagaagagct ctttgacaac caaaggcttt aagcagtaag aagagttgat
      241 tgaagtgtaa gatcactctc cttaaaccag gcatccatat gctgacacat cataatagca
      301 taaatgcgga tgttccacat tttagagctg cggtgcctgg cagcttccag catatagtca
      361 accttttctc gcttgtttga acgttcaacg gaggttctac gtttgtaggc gttcttccac
      421 tcattgctgt tgcgggcaat gggtgggaac aagcggatat tctctttggt gtaggtgtaa
      481 acacaacgac cgtaggaaga agtagagcaa ggagaatcgc aagcaacgat ccccttttta
      541 atcaaaggac agcggtattt acgacggccc cgagtataat catagccgtt atccttcatc
      601 tccaggcctt tgttgcaaat aggaatgcct gattcagaca aagaaaaatc atccttgtat
      661 ttggtctgcc ccgatcttct tttatttaaa tcaataaaag caagaatatc ccggttatta
      721 aaataatgat agataggcat ggcatcatga gccgcatcaa gaagaacgcg ggaaataacc
      781 cagtcaggat agcgcagctg taattcttca gcactaagta acatagaaat agaatcgtgc
      841 cttgaagcct tatacaaacg aggataaacc ggcagatcat aaaaactttc agcagcagtg
      901 tagacataca aatgatagcc atgaaaataa cattcacggg agctgtccca gccaatatta
      961 cagtccggct gggagtaaat acgcggacac tcacagtcaa aaataccctt ttcccggcag
     1021 tcacaagtac gtttactgcg aggataagcc gaagtgcgaa caggagttcc atcgccggcc
     1081 aaacaaagat ttttgatatc accaagaaga ccaagttgag ctgacttaaa aacaaaaatc
     1141 tccttaaaca aggcacaaag acgatcaaag ggaagaaact tgaacacagc agaacgacgc
     1201 tccagacctt taaccagatt ggcaacctta ttaggagaag tagtgggagc tttttccccc
     1261 ttcctcgcac cctttttaat ctttttggtt ttacgttttt tcttacagct gaaattaggt
     1321 gaatcacttg cccagagacg agcaaagaaa tcatagaaag taccaacacc aggagtctta
     1381 ttagggatga acccacttaa gatagcataa aggggaaccc gacgaagcag agtaacccat
     1441 tgtgttacag aagatacccc ggtttgtacc atgaggagaa tagatctgag catatcagcg
     1501 gggtcacgag gtcttggccc ctgtttagaa aaagtatctc taagaataaa agctgtttta
     1561 gacaaatctg taagccagaa cttttcgatt aggttccagt cgggggattg gatacaaaga
     1621 atacccccgg tgtaatgagt ttttaactgc tcggcaacaa aagactgata atcacagtgg
     1681 gatcgaacta caggtaacat aaaaacgcct ccaattgcga agatgagact aaagaaagcc
     1741 cctcatcgcg attatggagt tgtttccgaa aagtcaaggg ttttcgcaaa aaaagttaaa
     1801 aaattatcga caaatttatt tcacgattac attgattaag gttgtttgca tttatgggat
     1861 tatatgtaat ctgattgggg tgggtattgt ggaggaaaaa gcaaaagtag ctcagggata
     1921 aacccaggag ctacatacat cggcaaatgc cgagaatcta tttaagcgta aggaggatga
     1981 gatgtgtcgt acgattacca aatagttgac aacatcgtta tttttaagtt taaccggggc
     2041 aagctgaatt ccattaccat ggagacccta cagggactaa accaggtaat agatcgtgta
     2101 aatcatgaaa aggagctgaa aggcatcatc ctgaccggct aagccgttaa ttaccgccag
     2161 cttcttttta ta
//
LOCUS       DAOSWN010000079         2139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_41815_length_2139_cov_4.087332, whole genome shotgun sequence.
ACCESSION   DAOSWN010000079 DAOSWN010000000
VERSION     DAOSWN010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41815_length_2139_cov_4.087332"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..461)
                     /locus_tag="PLF29_03440"
     CDS             complement(6..461)
                     /locus_tag="PLF29_03440"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXK53015.1"
                     /translation="MIRKKEIRKAVRAIVTDAEHKILLVFDTYKNYYSLPGGGVEEID
                     RTDEEAIIREVMEETGYITRIIFDLDYYQEESEKRINKSHGFITKVINVKSKKLNMDE
                     QNFENVWMPLEEAILKIKQDLNNHNYSKFKSFFERELFFLEKAKIYLQN"
     gene            947..>2139
                     /gene="aspS"
                     /locus_tag="PLF29_03445"
     CDS             947..>2139
                     /gene="aspS"
                     /locus_tag="PLF29_03445"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF003483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HXK53016.1"
                     /translation="MERTFIKDIGSKVSKEVLLKGWVHTIRDQGSIKFVLLRDITGIC
                     QLIFTQENKEVFDKASGLSLESVVEVVGVAQEMPQAPKGLEIKATSIVTLSEAAAELP
                     IPVVEKGGGETEQPKRLDWRWIDLRKPEKALIFKVWTWMEHYIREYFINEGFFEIHSP
                     KFMSTPSESGAELFEVKYFDKTAYLAQSPQFYKQMAMASGFEKVFEVGPVFRANPSFT
                     SRHDTEFTGYDFEISFIESHYDVMEQEEKLLAYVLKKLEERFGQDIEHAYGRKLVAPK
                     TPFPKVSMQEAKEILAGLGVISEKDGDLSPEEERRLSEHILKEKGSEFVFITEYPVDV
                     RPFYHMRVEGDPALTKSFDLLWNGIEITTGAQREHRHDVLVAQAEEKGIGLESIKHYL
                     NFFKYG"
BASE COUNT          647 a          357 c          455 g          680 t
ORIGIN      
        1 ttcatttaat tttgtaaata tatttttgct ttttctaaaa agaataattc tcgttcaaag
       61 aaggatttaa atttagaata gttgtgatta tttaaatctt gttttatttt taatattgct
      121 tcttcaagcg gcatccatac attttcaaaa ttttgttcat ccatatttaa ttttttactt
      181 ttaacattaa tcacttttgt aataaaccca tgagatttgt ttattctttt ttctgattcc
      241 tcttgataat aatctaagtc aaatattatt cttgtaatat acccagtttc ttccatcacc
      301 tctctaataa ttgcctcttc gtctgttctg tctatttctt ctaccccacc tcctggcaga
      361 gaatagtaat ttttatatgt gtcaaatact aacaatattt tgtgttctgc atctgtgact
      421 atggctctta ccgcctttct aatttctttt ttacgtatca tttttgaatt ataacaaatg
      481 gaatactttt ataatttcgt tacttataac tacatgcatt agctagtaga tgcggtttgc
      541 ccagcatgtt gggttttgca gcgcaaagaa attacaggaa accgcccgct taagccgacg
      601 gtggctgatt tcgactattc tttagtttat gctataatgc tgacaatggc gaagattcgg
      661 ctaacaccga ggagcttgag gaagaaatct ttatagaaag tagaacctca cgggaactcc
      721 cgttacagag ttgcgccttg tccttaccgt aggtttaggg agaggcttaa aggcttgtct
      781 ggtaacagat aagcaaagtg aggtggcacc gtgtatcttt tgttaactgt aagttactaa
      841 gaaacaccct cgcaaggata tcaatttaat gtagtattaa attgttggta ttctagcgag
      901 ggtgtttttg ttttctctcg gaaaaatatc aactaaagga gtaattatgg aaagaacctt
      961 tataaaagac ataggctcta aagtttctaa agaggtccta ctgaaagggt gggtacatac
     1021 gataagagat caaggaagta ttaaattcgt cttgctaaga gatataacgg gcatttgtca
     1081 gctcattttc acgcaggaaa acaaagaggt cttcgataaa gcctccggtc tttctttgga
     1141 atctgtggtt gaagtagtag gtgttgcgca agaaatgccc caggcaccta agggcctaga
     1201 aatcaaggca acttcaatag taactttgtc tgaagcagcg gctgagctgc caatacctgt
     1261 agtggagaag ggcggcgggg aaacagagca gcctaaaaga ttggattggc gctggataga
     1321 cttgagaaag cctgaaaaag ccctaatctt taaagtgtgg acctggatgg agcactatat
     1381 aagagagtat ttcataaacg aaggtttctt tgagatacat tcgccgaaat ttatgagtac
     1441 tcccagtgag tctggagcag aactgttcga agtaaagtat tttgataaaa ccgcttattt
     1501 ggcacaatct ccgcagtttt ataagcaaat ggctatggca tctggttttg agaaggtttt
     1561 tgaggtgggt ccggttttta gggcgaaccc gtcttttaca tcacggcatg acactgagtt
     1621 taccggatat gattttgaga tttcatttat tgaatctcat tatgatgtaa tggagcagga
     1681 agaaaaactt ctcgcctatg tgttaaaaaa gctggaagag aggtttggac aagatattga
     1741 gcatgcctac ggtaggaaat tagtagctcc taagactccc tttccaaagg taagtatgca
     1801 agaagcaaaa gaaattcttg ccggtttagg agttataagc gaaaaggatg gagatttgtc
     1861 cccggaagaa gaaagacggt tgtcagaaca tattcttaaa gaaaaaggaa gcgaatttgt
     1921 ttttattact gaatatccgg tagatgtaag acctttttac catatgagag tagagggtga
     1981 tcctgcgcta acaaaaagct ttgatcttct gtggaacggt atagaaataa caacaggggc
     2041 acagcgtgag cacaggcatg atgtgctggt tgcacaagct gaagaaaaag gcataggtct
     2101 agagtctata aaacactatc ttaacttttt taagtacgg
//
LOCUS       DAOSWN010000080         2084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_43645_length_2084_cov_4.993593, whole genome shotgun sequence.
ACCESSION   DAOSWN010000080 DAOSWN010000000
VERSION     DAOSWN010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43645_length_2084_cov_4.993593"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="PLF29_03450"
     CDS             complement(<1..701)
                     /locus_tag="PLF29_03450"
                     /inference="COORDINATES: protein motif:HMM:NF019138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK53017.1"
                     /translation="MPTAKKVHSDRDQIAQKNSDSSINTDGSNGSAVVEAKSLSDSST
                     SNNPESKNNTSVSKTDQDDVSAVRTTVITTESRNNKSHQSRGRHSSGSSGNNGGNSGT
                     TNSSGNSKFFTGETIKVEGVLELFTDYGVIRQDIADTGDNDLPKDVYTSLSQIKRFSL
                     RMGDLVEGYARAPKEGERYLSLLQIEKVGGLDPSEAKKRPRFSKLTPIFPDEWMKLET
                     APEILSTRIIDLVSP"
     gene            complement(840..1634)
                     /locus_tag="PLF29_03455"
     CDS             complement(840..1634)
                     /locus_tag="PLF29_03455"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class F sortase"
                     /protein_id="HXK53018.1"
                     /translation="MAKKFSKKQFRRLVRITILLYFIVGMEAAVIYSKYNKNDANIVS
                     ASKPFYSQDLLAANVLGLSSRHGKDVVVEDLDVDSETSSTNPVSANSTEQEISTSSVF
                     ESILGSITSTPSRLEISSQGINIPLVQVGVSSSGSMETPVDWNSGGWYSRSAKLGAFG
                     NTIINAHYDNNFGAPAAFWRLQNVAVGDTVGVFDELGRRFDYVVSETFYVDIQDPERL
                     KIFDEPSAGKSELTLVTCGGVWVPSEGTYNKRLVVKAEYVGFSFVD"
BASE COUNT          646 a          468 c          393 g          577 t
ORIGIN      
        1 ataggggaaa cgaggtctat gattcttgtc gataagattt caggagctgt ctccagcttc
       61 atccactcat caggaaaaat gggggttagt ttagaaaatc ttggtcgttt ttttgcctcg
      121 gaaggatcta acccacctac cttttctatt tgcaacaatg ataaatatct ctcaccttct
      181 tttggtgcac gagcatagcc ttcaaccaaa tcacccattc ttaaactaaa tctttttatc
      241 tgcgaaaggc tagtataaac atctttcggc aagtcgttat ctcctgtgtc tgcaatatcc
      301 tgcctaataa caccgtaatc tgtaaaaagc tctaaaacac cctcgacctt aatagtttcg
      361 cctgtaaaaa acttgctatt tccagaagaa tttgtagtcc cagagtttcc tccgttgttt
      421 ccggaagaac ctgacgagtg ccttccacga gactggtggg atttattatt cctagattcg
      481 gttgtaatta ccgttgttct aacagcagaa acgtcgtcct gatcagtctt tgagactgag
      541 gtattatttt tcgactcggg gttattggac gtcgagctat cggataagct ttttgcttct
      601 acgactgcgg acccatttga gccgtccgtg ttaatagagc tatcgctatt tttttgcgca
      661 atctgatctc tgtcagaatg aacttttttt gctgtaggca ttttttatct aacctttcgg
      721 aaatttacaa acaatagtta tatgcactct taggtcggga gatgaccact gccccgagtc
      781 acctcccaac ctaaaaaaac atatcgtcaa acaaacacaa aaaacactaa acaaattgcc
      841 tagtctacaa aagaaaagcc aacatactcc gctttaacaa caaggcgctt gttatatgtt
      901 ccttctgagg gcacccatac cccgccacaa gtaacaagtg ttagctcaga cttccctgct
      961 gaaggctcgt caaatatttt taacctttct gggtcttgga tatcaacata gaaagtttct
     1021 gacacgacat aatcaaaacg ccggcctaac tcgtcaaaca cacctactgt atcacctaca
     1081 gccacatttt gcaacctcca gaaagctgcg ggagcaccaa aattattatc gtagtgagcg
     1141 tttataattg tgtttccaaa agcgcctaat tttgcagatc tggaatacca tccaccacta
     1201 ttccaatcca ccggagtttc catagagccg ctcgaagaaa caccaacttg taccaaagga
     1261 atatttatac cctggcttga aatctcaagc cttgaggggg tcgaagttat tgaacctaaa
     1321 atactttcga aaacggaact ggttgatatt tcttgttcag tgctgttagc agagaccggg
     1381 tttgtagaag aagtctcaga atcaacatct agatcttcga caacgacgtc tttcccatgc
     1441 ctggaactca aaccaagtac gttagcagcg agtaaatctt gtgaataaaa aggcttcgat
     1501 gctgaaacga tattagcatc attcttatta tacttactat atattacagc ggcctccata
     1561 ccaacaataa aataaagcaa tattgtgata cgaactaacc ttcgaaactg ttttttggaa
     1621 aatttttttg ccatatgaag tactcctgta aacctcgcta taaggcctta ggaataaaat
     1681 agattggtcg cgtaagggcg taaccaaacc caaccaatct actctatctc taaaaccata
     1741 aatagaaggt gcaggacaaa agctttttta tttgttactt ggcaggaata aaaattacaa
     1801 taaatataat aagttattaa actattgtta atgatgcatt atacactata aggtaaaaaa
     1861 tgcaagagaa aaaaacaaaa ctggtcccgc cggtgcgggg ggggggggtt ttagagtcgc
     1921 tttgcgaccc aagcccctcc cgagagaaac agtacgatcg caagagcata cacaaggctt
     1981 gcctcgagcg agagctcagg gacaaaaaat actaaaaaaa cagccgacaa caaaacaagc
     2041 aacagtacga gggcgtacgc tgcagtcttg gtggccgaga ccgg
//
LOCUS       DAOSWN010000081         2053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_44753_length_2053_cov_4.174174, whole genome shotgun sequence.
ACCESSION   DAOSWN010000081 DAOSWN010000000
VERSION     DAOSWN010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44753_length_2053_cov_4.174174"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PLF29_03460"
     CDS             <1..182
                     /locus_tag="PLF29_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK53019.1"
                     /translation="TPLPERERDTAKMLHDSLARPIFQYKTVSVNASNSTSMNMPILR
                     NPEDRPELYEGEEDE"
     gene            249..467
                     /locus_tag="PLF29_03465"
     CDS             249..467
                     /locus_tag="PLF29_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK53020.1"
                     /translation="MKPKKNQDNKIVGLYRNTKDGGKTWEFKCVLLHGDPIDITESEY
                     VALKSSRVEIPSHDIKLDSVVYGFSVTF"
     gene            477..731
                     /locus_tag="PLF29_03470"
     CDS             477..731
                     /locus_tag="PLF29_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK53021.1"
                     /translation="MKKKILENWIHIEAIESEMKEDTGDSLAGFNRGLLFNGQRMLLI
                     PLEHKRLQKNGLMTKKVYTRYFLANYCPFSGLPLYEEEAE"
     gene            772..1362
                     /locus_tag="PLF29_03475"
     CDS             772..1362
                     /locus_tag="PLF29_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK53022.1"
                     /translation="MKNRNFEKVQRQIQDVLPHLGIFVIIAVYMVTAVSMGNFFHMRM
                     STAGALPYFIAYGTGFGTQLTRAYIVFNSQISYGNPQSSTFAYLFGALLTVYTGMEAY
                     HLASDYFVSILHIVGSGYIVEILYLRHLDTASAYQLTKDPTLLNEFIEFERKQRELRD
                     FMKGEDNYDKGRKRGVRFDYEAEEVPFEYSENGKHK"
     gene            1533..1958
                     /locus_tag="PLF29_03480"
     CDS             1533..1958
                     /locus_tag="PLF29_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK53023.1"
                     /translation="MKITENRLEVIRTQQEYIDSFHERGGLTPSKPASMKNSVYQILC
                     RMYLLAGELPIGTKVKIYIGDVCEDQYDLKKTVEYTISKLKQWHDVPCLFTQTAWLTF
                     LQYEIKFVIGYDKQKEYVAQPHSIVVYRQTPDFLQKRFF"
BASE COUNT          662 a          398 c          439 g          554 t
ORIGIN      
        1 ctactcccct accggagaga gagcgtgaca cggcgaagat gttacacgac tccttagccc
       61 gtccaatttt tcagtacaaa actgtatccg tcaatgcatc aaattccacc tctatgaaca
      121 tgccgattct taggaatcct gaggaccggc cagaactata cgagggcgaa gaagatgaat
      181 aattcaatcg gcctaacttt gtcatgcaaa gttaggtctt ttttttgact agtctaaaaa
      241 tcaatttaat gaaacctaaa aaaaatcaag acaacaagat cgtcggatta tacagaaaca
      301 caaaggacgg cggcaaaacg tgggaattca aatgtgttct tcttcatgga gatcctattg
      361 acataacaga gagcgagtat gttgcattga agtctagcag agttgaaatt cctagccatg
      421 acataaaact agatagcgta gtttatggct tttcggtaac tttttaacat aaaacaatga
      481 aaaagaagat attagagaac tggattcata tagaggctat agagtctgaa atgaaagagg
      541 atactggtga tagtcttgcc ggctttaatc gaggtctttt gtttaatgga cagaggatgc
      601 tattgatacc tttagagcac aagaggttgc aaaaaaatgg cctaatgact aaaaaggtgt
      661 acactaggta ttttttggct aactactgcc cttttagtgg ccttccgttg tacgaagaag
      721 aagcggaata gtgatacatt actgattatt aacttttaaa cagctaacaa catgaaaaac
      781 agaaattttg agaaggttca aagacagatc caagacgttc tacctcatct tgggatcttc
      841 gttatcattg ccgtctacat ggttacggcc gtatcgatgg ggaacttttt tcacatgaga
      901 atgagcacag cgggcgccct accctacttt atagcgtacg ggaccggatt cggcacgcaa
      961 ctgaccaggg cttacattgt gttcaattct caaataagtt acggcaaccc acaaagcagt
     1021 acgtttgcat atttgtttgg tgcgctccta actgtttaca ccgggatgga ggcttatcat
     1081 ttggcttcag actactttgt atcaatcctc cacatagttg gttccggtta tatcgtggaa
     1141 atattgtacc ttagacatct ggatacggca agcgcctatc agctgactaa agatcctacc
     1201 ctactcaacg agtttattga atttgaaaga aagcagagag aattgaggga tttcatgaag
     1261 ggcgaagata actacgacaa gggtagaaag agaggggtaa gattcgacta cgaagcggaa
     1321 gaagtccctt tcgagtattc ggagaacgga aagcacaagt agaaatatca ttggtatgtt
     1381 tgaattgtgg ccgggatcta tcctataggc cggatctaag ggccacaatt cgtttttgtt
     1441 ctccgaggtg taggaagcga cacaactact tgacttccaa gcaaaatagc gtccaatcta
     1501 aacaattact ttaatcaaaa aatatctatc ctatgaaaat tacagaaaat agacttgaag
     1561 taataagaac gcaacaagag tatatagaca gcttccatga gcgggggggg ctaacgccat
     1621 ccaagccggc ttcaatgaaa aactcagttt accagattct ttgcaggatg tatttattag
     1681 ccggagagct tccgataggg actaaggtta agatttatat tggtgatgtt tgcgaagatc
     1741 agtacgatct aaaaaagacg gttgagtata ctatctcaaa actaaaacaa tggcacgacg
     1801 taccatgctt atttactcaa acggcttggc ttacgttcct tcagtacgaa ataaaattcg
     1861 tgatcggata cgataaacag aaagagtacg tagcccagcc gcatagtatt gttgtatata
     1921 gacaaacacc tgacttcctg caaaaacgtt tcttctagtt cgcttttcca tgtagcccgt
     1981 acggttttct gtacgggctt attaaaatca catcgatatg gcaaagcgta cctacaaaaa
     2041 aatatcatcc tat
//
LOCUS       DAOSWN010000082         1948 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_48839_length_1948_cov_3.893819, whole genome shotgun sequence.
ACCESSION   DAOSWN010000082 DAOSWN010000000
VERSION     DAOSWN010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48839_length_1948_cov_3.893819"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            331..1275
                     /locus_tag="PLF29_03485"
     CDS             331..1275
                     /locus_tag="PLF29_03485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXK53024.1"
                     /translation="MNEFKVLGVIVSYNTRATLDECLKNLVEIKNSAPAKFLEIVVYD
                     NASNDGSAEMVETKYPSVKIMKGSENLGISAAANAAAFAFTSDYILYLGSDAFPSLET
                     VAGLSEYLDAHPKVALATTALIKNDGSLDLDAHRGIPTPFAALSYFLGFSRLFPSSKV
                     FNRYFMGWEDLAKPHEIEACISHFMFVRRKALFEVGGWDEDYFVFGEDIDLCYRLRSA
                     GYKIMYLPQYKTLHLKGVSVGIRPETSEKVSVPAKVKRRMVRQTTKAMRMFYKKNMSK
                     DYPFFMLLPIYFATFALEVWRITSLELKLIKSSIYKRL"
     gene            1319..>1948
                     /locus_tag="PLF29_03490"
     CDS             1319..>1948
                     /locus_tag="PLF29_03490"
                     /inference="COORDINATES: protein motif:HMM:NF016013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HXK53025.1"
                     /translation="MLLGSFITNDLFHVNKAEILKKVRHSTEFSFSFILLLIGSSIVC
                     TLGLLLNSTPIVIGGMIISPMMWPLMQTSIGVAYGRSSYVRQAFLLLVFSIVASLTAS
                     VLITFLSPIKLLNPEILSRTYPTLLDLIVAIVAGGVAALAISQPKISESLAGVAIATS
                     LMPPLCVSGIGLALMRPDIFLGSFLLFLANVISIIFISIIVYLLLGLRAK"
BASE COUNT          543 a          332 c          393 g          680 t
ORIGIN      
        1 ggtatataaa gcttttgttt catctccgta atagtttgaa taccctaaat tactaaaacg
       61 aggcggcagg gctaaaagca agatgactat tactggtatt attattttaa gtttttttct
      121 catgttttat aacttcgtta attaaaacta ttgcgcaagc tatagatgta gtttacccaa
      181 catgttgtgc tttgtaacgc aaaaaactac agcaaccacc tgcgtaagct ggtggtggtt
      241 gatagtttaa ataataacat tttagaatag tccatagaat cgttctcgtt tgttatggaa
      301 gtttaattgt tttacatata aaatagcatt atgaatgagt ttaaagtgct gggcgttatt
      361 gtgagctaca acacgcgagc tacacttgat gagtgtttaa aaaatctagt tgaaataaaa
      421 aatagtgccc cggcaaagtt tttagagata gttgtgtatg ataatgcttc taatgacggc
      481 tcggctgaga tggtagaaac aaaatatcct agtgtaaaga taatgaaagg tagtgagaat
      541 cttggcatct ctgccgcggc taatgccgcc gcttttgctt ttacatctga ttatatttta
      601 tatttaggca gtgatgcttt tccgtctttg gagactgtcg caggtttatc ggagtatctt
      661 gacgcacatc ctaaagttgc ccttgctaca acagcgctaa ttaagaatga cggttctcta
      721 gatctggatg ctcacagagg tattccaacc ccttttgcgg ctctttctta tttcttaggg
      781 tttagcaggc tgttcccttc ctccaaagta tttaataggt attttatggg atgggaagat
      841 ttagcaaagc ctcatgaaat cgaggcatgc attagccatt ttatgtttgt tcgtaggaag
      901 gcattatttg aggttggggg atgggacgag gattattttg tttttggcga agatattgat
      961 ttatgttatc gccttcgtag tgcaggatat aaaattatgt acctcccaca atataaaacc
     1021 ttgcatttaa aaggcgttag cgtgggtatt cggcctgaaa catctgaaaa agtttcggta
     1081 cccgctaagg taaagagacg aatggtaagg caaactacaa aagccatgag aatgttctat
     1141 aagaaaaata tgtctaagga ctaccccttt tttatgctct tgcctatata ttttgctaca
     1201 tttgctttag aggtttggcg aataactagc ttagaactaa agttaattaa gagcagtatt
     1261 tataagcgtc tctagagtta ccgaggtagc gtccgtttaa atatgttatt cttaatttat
     1321 gttattaggt tctttcatca ctaatgacct ttttcatgta aataaagcag aaattttaaa
     1381 aaaagtccgt cacagcactg agttttcatt cagttttatt ctattattaa tcggctcaag
     1441 cattgtatgc acgctcggac tacttttaaa ctcaaccccc attgttatag ggggtatgat
     1501 tatctctccg atgatgtggc ctttgatgca aacctcaatt ggagtcgcat atggtagaag
     1561 ttcttatgta aggcaggcct ttttattgtt agttttttca atagtcgcaa gcctaacagc
     1621 ttctgttttg ataactttcc tctcccctat taaactgctg aatcccgaaa tattatcaag
     1681 aacttaccct accttgttgg atttaattgt agctattgtg gccggaggtg ttgcggcttt
     1741 agcaatttct cagcctaaaa tatctgaaag tttggccgga gttgctattg ctacgtcgtt
     1801 aatgccaccg ctctgtgttt caggtattgg tcttgcttta atgaggcccg acatattttt
     1861 aggaagcttt ttacttttct tagctaatgt catatctatc atatttattt ctattattgt
     1921 gtacctgctt ttgggtttaa gagcaaaa
//
LOCUS       DAOSWN010000083         1912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_50343_length_1912_cov_4.647281, whole genome shotgun sequence.
ACCESSION   DAOSWN010000083 DAOSWN010000000
VERSION     DAOSWN010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50343_length_1912_cov_4.647281"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="PLF29_03495"
     CDS             <1..586
                     /locus_tag="PLF29_03495"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HXK53026.1"
                     /translation="LHLMPEGVRSLDPVLFFRITLVSFVAFFVVEKIIHWHHCHDSDC
                     DVHSFGYMNLFGDAVHNFMDGLIIALAFLTSPVIGVTTTLALALHELPQEIGDFGVLL
                     HAGFSAQKALFYNFMVGLFSLLGGVLGFFLLSRTPSIISYLLPVATASLLYVATSDLL
                     PELKNETGLKNTFILTSIIFFGICLMYVLSLYSG"
     gene            723..998
                     /locus_tag="PLF29_03500"
     CDS             723..998
                     /locus_tag="PLF29_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK53027.1"
                     /translation="MQNSKIKTLFNQNATNIRQLKLIITELSSKIHEKLRILFLYGRF
                     STDKNSKRTFGFFFLFYIALIFSSLSNKLGNNFALVSTKNGELYAGY"
     gene            985..>1912
                     /locus_tag="PLF29_03505"
     CDS             985..>1912
                     /locus_tag="PLF29_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK53028.1"
                     /translation="MQDINMTVQGSPVSISYKEHFSNLVKVAEALSFGFSEHYRSSGL
                     TYVDAPEIVGITGACENVDTLFRVKGKKDIPLFFTQTGQLSLEQALQSFSGVFTVIHS
                     GRDEDKEDSRHLRQFRLTEEEFDCTMIGMTRDSYDEDAMYEALLSHIEAAIKSGISSV
                     LKCTPEILERYGRDISTLENAIIQDFHRISYEDSIKLLNDNGYPELVFGDDLKEHHEA
                     EIIKLMNSSEPRLPVFIMKYPEEIKFFNMKEYTKDPKVVLSADLILPYSGESVGSAVR
                     EHDYEKLNDRLLRSTMYSLHVKRGGSYKDYEWY"
BASE COUNT          552 a          339 c          381 g          640 t
ORIGIN      
        1 tttacatctt atgccggagg gagttagatc gctggatcct gtactttttt ttagaatcac
       61 tctagtttct tttgtcgcat tttttgttgt tgagaaaata atacattggc accactgcca
      121 tgacagcgac tgcgacgtgc actcttttgg gtacatgaac ttatttggag acgcagtgca
      181 taattttatg gacgggctca ttattgcctt ggcattttta acaagtcctg ttattggagt
      241 aactactacc cttgccttgg ctttacatga gttacctcaa gaaataggag attttggagt
      301 attgttgcat gccggattta gtgctcagaa ggcgttattc tataacttta tggtgggtct
      361 tttctctttg ttggggggtg tgcttggttt ttttctactt agcagaacgc cttctataat
      421 ttcttatcta ttacccgttg ctacggcgtc tttattatat gttgccactt ctgatcttct
      481 gcctgaacta aaaaatgaaa ccggcctaaa aaacactttt attttaacct caattatctt
      541 tttcggtatc tgtctaatgt atgtgctgtc actttactct ggttaaggtt tgttaaataa
      601 gaacatgcaa aaagctaata agacatgaag ctttcttatt acgtgctttg gcgccattgc
      661 tataataact aatttgcact tgttgcataa ttcaaatata tgctatattc tagaatttta
      721 gaatgcaaaa ttctaaaatt aagaccttat ttaatcaaaa cgctacaaac attcgtcaac
      781 taaaacttat aattacggaa ctctcctcta aaatacacga aaagttgcgt attttgtttt
      841 tatatggccg tttttctacg gacaaaaatt ctaaaagaac cttcgggttc ttttttttat
      901 tttatatagc tttgatcttt tcttcactat caaacaaatt aggtaataat ttcgcgcttg
      961 tgagcactaa gaacggagaa ttgtatgcag gatattaaca tgacagttca aggaagcccg
     1021 gtatctattt cttataagga gcacttttca aacctcgtaa aggttgctga ggctctttct
     1081 tttggttttt cggaacacta cagaagttca gggcttactt atgtagacgc cccggaaatc
     1141 gttggaatta ccggtgcttg cgaaaatgta gatactttat ttagagtcaa gggtaaaaag
     1201 gacattccgc tattttttac acagaccggt cagttatctc ttgaacaagc gttacaaagt
     1261 tttagtggtg tatttactgt tattcattcc ggcagagacg aggataaaga agactcccgt
     1321 cacttaaggc agtttagatt gactgaagag gagtttgatt gcacgatgat aggaatgacc
     1381 agggatagct acgacgaaga cgcaatgtat gaggcgctgc ttagtcacat tgaagcagcc
     1441 ataaaatccg gtatttcttc agttttgaag tgtaccccgg agatacttga gcgttatgga
     1501 agagatattt ctacgcttga gaacgcgatt atccaagatt ttcatagaat tagttatgaa
     1561 gattcaatta agcttctgaa tgataatggt taccctgagt tagtttttgg agacgatctt
     1621 aaagagcacc acgaggctga aataataaaa cttatgaact cgtcagagcc tcgtttgccc
     1681 gtatttatca tgaaataccc cgaagagatt aaatttttta acatgaagga gtatacaaaa
     1741 gatccaaaag tcgtgctgtc agcagattta atacttccat attccggtga atctgttggt
     1801 tcagctgtca gagaacatga ctatgagaag ctgaatgata ggctgttaag gtccacaatg
     1861 tattccttac acgtaaaaag aggcggctct tacaaggact atgaatggta cc
//
LOCUS       DAOSWN010000084         1886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_51603_length_1886_cov_2.709995, whole genome shotgun sequence.
ACCESSION   DAOSWN010000084 DAOSWN010000000
VERSION     DAOSWN010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51603_length_1886_cov_2.709995"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PLF29_03510"
     CDS             <1..183
                     /locus_tag="PLF29_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK53029.1"
                     /translation="ILYKEQSADAIKEVINRYSKNKFNPDLCIVRAENFSKEIFSRIV
                     KDYVDSVYSDGHVFSN"
     gene            161..796
                     /locus_tag="PLF29_03515"
     CDS             161..796
                     /locus_tag="PLF29_03515"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HXK53030.1"
                     /translation="MAMYFRIKRLTDIVCAIIGIVVFSPVILIASLFIKSVSPEGSIF
                     VDSKPRVGKDGKEFKMYKLRTMRPNAQKWLETQPELYKRYQENGYKLDPDPRLLKGGK
                     FIRKYSIDEMPQFFNVLKGEMSVVGPRAYFKYELEEQEGRYPHTKDYIKQALTTKPGL
                     TGPWQIGGRSNVNFEERIKMDSEYASRHSVLYDLIILLKTPYVVITGSGAV"
     gene            812..>1886
                     /locus_tag="PLF29_03520"
     CDS             812..>1886
                     /locus_tag="PLF29_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK53031.1"
                     /translation="MDSKFFASSKTGLKPASAKGTQLYGNKNNKKILKGAGLAAGIAA
                     VVFLILFAVTYFVALRPTLALTSKVNEVKADISEISKSATNRDLVELSANLDKLEMDI
                     AELRAARDENIGWMENFGLTKEYYADSNHFMDAGLQMIEAGREAIKLIEPFADAGGFR
                     ISAEQEIEIVDPAQGSGLAEAFSNWIAIMPEIAGDIDVVLNRLTLAGEELNKVDASKY
                     PESFRGTDLRQSIIKAQNTLTLLNDSAPDIKEALNIIPPLLGVGTTEKRYMILMENDK
                     ELRATGGFWTYISTFKIANAQLSSDFTSQGTYNIDFALEVIDPYYTFPTVPDAYRNHL
                     KVERMFSRDANISPDFPTSVDQFM"
BASE COUNT          618 a          334 c          413 g          521 t
ORIGIN      
        1 attctttaca aagaacaatc cgctgatgcc ataaaagagg tgataaatag atattcaaag
       61 aataagttta atccagatct ctgtattgtt agagcggaga atttcagtaa agaaattttt
      121 tcgaggatag taaaagacta tgtagatagt gtatactcag atggccatgt attttcgaat
      181 taaacggctt acagatattg tttgtgcaat tatagggatc gttgtttttt ccccggtaat
      241 attaatagct agccttttta ttaaaagcgt ttctcctgaa ggctcaatat tcgttgatag
      301 taagccaaga gtcggaaaag acggaaaaga gtttaagatg tataagctta gaactatgcg
      361 cccgaacgca cagaaatggc tggaaacaca gcccgaactc tacaaaagat atcaggaaaa
      421 cggttacaaa ctagatcccg accctcgttt attgaaaggc ggtaaattta ttagaaaata
      481 cagcatagac gaaatgcccc aattctttaa cgttctcaaa ggcgagatga gtgttgttgg
      541 tcctagggcg tattttaaat atgagcttga ggagcaggag ggcagatatc cccacactaa
      601 agactacata aaacaagccc taacaacaaa gcccggatta acaggtcctt ggcaaatagg
      661 cggaagatct aacgtcaact ttgaagaaag aataaaaatg gattcagagt atgcaagtag
      721 gcactctgtg ttgtatgatt taataatatt attaaaaacc ccatacgttg taataaccgg
      781 aagtggggcg gtttaatttt aggagattaa tatggattca aaattttttg cttcgagtaa
      841 aacaggcctt aagccggcct ctgcaaaagg cacccagctt tatggtaata aaaacaataa
      901 gaaaatacta aagggcgctg ggttggccgc aggaatagca gcagtggttt ttctgatact
      961 ttttgctgta acgtactttg ttgcactaag gccaacactt gcactaacgt caaaagtaaa
     1021 cgaggttaag gccgatattt ctgagatatc aaagtccgct actaacagag accttgttga
     1081 gctttctgca aacttagata agctagagat ggatattgct gaactaaggg ctgcaagaga
     1141 tgagaatatt ggttggatgg agaattttgg cctcacaaaa gagtactacg cggactcgaa
     1201 tcatttcatg gacgcaggct tgcaaatgat tgaagccggt cgtgaggcta taaagctaat
     1261 tgagcctttt gcggatgcag gtggttttag aatctctgca gagcaagaga ttgaaatagt
     1321 tgaccctgcc caaggatcag gtttggcgga agctttttca aattggatag cgataatgcc
     1381 cgaaatagct ggtgacatag atgttgtcct aaacaggctc actctagcag gagaagagtt
     1441 aaataaggtt gatgcgtcaa aatatcctga aagttttaga ggtacagatt taaggcagtc
     1501 aataattaaa gctcaaaaca ccttaactct tttaaatgat tccgctccag atattaaaga
     1561 agcgttgaac attatccctc cccttcttgg cgtaggtacg accgaaaaga gatacatgat
     1621 actaatggag aatgataaag agttacgagc aacgggaggt ttttggacat acatctcgac
     1681 attcaagata gcaaacgcgc aactttcttc agattttacg tcacaaggta catacaatat
     1741 agactttgct cttgaggtta tagatccgta ttacactttc cccacggttc ccgatgctta
     1801 cagaaatcat ttgaaggttg aaagaatgtt ttctcgcgac gcaaatattt ctcctgattt
     1861 tcccacatcg gttgatcagt ttatgt
//
LOCUS       DAOSWN010000085         1841 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_53714_length_1841_cov_3.506159, whole genome shotgun sequence.
ACCESSION   DAOSWN010000085 DAOSWN010000000
VERSION     DAOSWN010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53714_length_1841_cov_3.506159"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PLF29_03525"
     CDS             complement(<1..336)
                     /locus_tag="PLF29_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK53032.1"
                     /translation="MSKNKVGYTDYRPARDNCFNPANRRDGDRGRIHRSLTSLRLGRL
                     LETPRGPCEAEAPEETKLTFYLFKHLFQQMPNMLNAIEEKPDLLPETEDRWGLWDEIK
                     RRELDHFVRF"
     gene            405..1073
                     /locus_tag="PLF29_03530"
     CDS             405..1073
                     /locus_tag="PLF29_03530"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXK53033.1"
                     /translation="MKILVIEDDIKIANSLKKGLEMEMFAVDVCYDGECGLDMALTED
                     YNIIVLDLMLPKVSGLDICTKIRKNNISVPILMLTAMSEIDDKVSGFEMGADDYLTKP
                     FAFDELVARIKALLRRPKNIAPQIFEVGSLKLDVQQKTISFNRKLLELSKKEFMLLEM
                     LIKNKGKVLSKDKLIQNVWDFESDVMPNTVEVFIASLRSKLGKAGCNYIKTVRGFGYK
                     LEPQ"
     gene            1070..>1841
                     /locus_tag="PLF29_03535"
     CDS             1070..>1841
                     /locus_tag="PLF29_03535"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HXK53034.1"
                     /translation="MILRKTKFKVTFWYVITVALTLLIFSGIFYFLTLSISERAIDRH
                     QRRIDMAFELNSDPGPGKGPGAQRSNAQLALDQAKQEGLKQLQKSLFIEIVALDIVII
                     LITAFFGYYIAGKSLSPVEVMIEKQKDFVSNAAHELKTPLTAMKAELEVAMRDKRLTR
                     AEISEVFSSTIEEINRLAALIGAFLNQSKYEKLNAATYKLTSLNSIVENVVAKNFILA
                     NSKRISVEMDLQDIDVKCDRASIYELVSVLLDNAIKYAF"
BASE COUNT          565 a          318 c          388 g          570 t
ORIGIN      
        1 gaatctgacg aaatgatcta actctcttct ctttatttca tcccataatc cccaacgatc
       61 ttcggtctcg ggcagtaaat ctggtttttc ctcaatagcg tttaacatat taggcatctg
      121 ttgaaataaa tgtttaaaca agtagaaggt tagttttgtt tcctctggcg cttctgcctc
      181 acaaggtccg cggggtgttt ctaataatct acctaagcgc agcgatgtaa gtgacctgtg
      241 gatcctccct ctatcgccat cgcgtctatt tgcagggttg aaacaattgt ctcgggcggg
      301 acgataatca gtatatccaa ctttattttt actcatagta tttttatcct tatctatttt
      361 aaacaagcgt aacatatatt ttattatttt gttaaactat acacatgaag atactggtga
      421 ttgaagatga cattaagatc gcaaattctc tcaaaaaagg cctagaaatg gagatgtttg
      481 ctgtggatgt ttgttatgac ggcgagtgtg gcctagatat ggctcttact gaggattata
      541 atattatcgt tttggatctt atgctcccaa aagttagcgg gctggacatt tgcacaaaga
      601 tcagaaaaaa taacatatca gtcccgatac tgatgctcac agctatgtca gaaatagatg
      661 acaaggtttc aggctttgaa atgggtgcag atgactattt aactaagcct tttgcgttcg
      721 atgagcttgt tgcaagaatt aaggccctgc ttcgaaggcc aaaaaacatt gcgcctcaga
      781 tttttgaagt tgggagtctg aagctggatg tccaacaaaa gacgatttct tttaatagaa
      841 agcttctgga gttgtcaaag aaagaattta tgttgcttga gatgttaata aagaacaaag
      901 gtaaagtttt atctaaggac aaattaattc aaaatgtgtg ggattttgaa tctgatgtaa
      961 tgccaaatac ggttgaggtt tttatagcct ctttgagatc aaagctagga aaggccggtt
     1021 gtaactatat taaaactgtc aggggttttg gatataagct ggagccacaa tgatattaag
     1081 aaaaacaaaa tttaaagtta ctttttggta tgtaattaca gtcgcactca cccttttaat
     1141 ttttagcgga attttttatt ttttgaccct atcaatctct gagagagcga tagacaggca
     1201 ccagcgaaga atagacatgg cttttgaatt aaactctgat cccggtccgg ggaaaggtcc
     1261 cggagctcaa aggagtaacg cgcagttagc attagaccag gccaagcaag aggggctgaa
     1321 acagctacag aaaagcttat ttattgaaat tgttgcccta gatattgtca ttatcttaat
     1381 tacagccttt tttggctact atatagccgg aaaatccctt tcgccggtag aagtcatgat
     1441 agagaagcag aaagattttg tctcaaacgc cgcccacgaa cttaaaacac ctttaactgc
     1501 aatgaaagct gagcttgagg tagcgatgag agataaaaga cttactagag ccgagatttc
     1561 agaggttttc tctagtacta ttgaggagat taaccgttta gcagcactta ttggcgcatt
     1621 tttaaatcaa agcaagtatg aaaagttaaa tgccgcaacg tataaattga cgagtttgaa
     1681 ctctattgtg gagaatgtgg ttgccaaaaa ctttattcta gcgaattcaa aaagaatttc
     1741 tgttgagatg gatttacaag atatagatgt gaaatgtgac cgcgcatcaa tttatgagct
     1801 tgtatctgtc ctacttgata acgctatcaa gtatgcgttt g
//
LOCUS       DAOSWN010000086         1796 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_55948_length_1796_cov_3.695003, whole genome shotgun sequence.
ACCESSION   DAOSWN010000086 DAOSWN010000000
VERSION     DAOSWN010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55948_length_1796_cov_3.695003"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PLF29_03540"
     CDS             <1..412
                     /locus_tag="PLF29_03540"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HXK53035.1"
                     /translation="CGKIDDVWRDDNGTLYIVDYKAVATSDSYKLTMEYKYNQGYKRQ
                     IEVYQWIFRQNGFNVSDTGYIVFVNAQTDPDYFDNKLEFLLSIEEVRGDCFWVEKELE
                     NIRACIDKTSIPESSQDCDICQYYNKRLMATNHK"
     gene            complement(418..>1796)
                     /locus_tag="PLF29_03545"
     CDS             complement(418..>1796)
                     /locus_tag="PLF29_03545"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXK53036.1"
                     /translation="TQKQSKNRRYSSAPSGARPGGFGRRNGGYKGKSKFSTSSGSDGE
                     SRGGQRSGNSFGYINRSGEQQFSKQGRSRDNWKRKNRRKSSEVIDHSRYISKPVQAQL
                     VEACVIENSFRDFNLHPTLLKNISDRGYIQPTKIQDMSIPHILKGRDVLGTGNTGTGK
                     TGAFLIPMCQKVLKNPENRVLIITPTRELASQIQVEFRKFTRDTKLRMVLVIGGESMH
                     FQMRALREKPQFVVATPGRLMDLEKRNQLSLKGFTNIILDEVDQMLDMGFVEDIKIVC
                     GKLPENRQSLFFSATMAGKEEALANTLLRDPVKVEADKQSPLNSIHQDIVKVASKEHK
                     IQILHDLLKKTEFTKVLVFSGTRIGADRITQELRSRGVMVDSLHGNKSQRVRTKVLTN
                     FRTDKIDVLVATDVAARGIDVPDISHVINFDEPVNYNDYIHRIGRTGRVGKKGTALTF
                     VVSKSFSK"
BASE COUNT          502 a          371 c          326 g          597 t
ORIGIN      
        1 gtgcggaaaa atcgatgatg tatggagaga cgacaacgga actctctaca ttgtggacta
       61 taaagctgta gcaacttctg acagctataa acttactatg gaatacaagt ataaccaagg
      121 ctataaaagg caaatagaag tttatcaatg gatattcaga caaaacggct ttaatgtctc
      181 tgacaccgga tatatagttt ttgtaaacgc tcagacagat ccggactatt ttgataacaa
      241 acttgaattt ctgctatcta tagaagaggt aagaggggac tgcttttggg ttgaaaaaga
      301 actagagaat attagggcgt gcatagacaa aacatcgata cctgaaagtt cgcaagattg
      361 cgatatttgc caatattaca acaaaaggct tatggcgact aaccacaaat agaaagacta
      421 tttagaaaaa cttttactaa caacaaacgt aagtgctgtt ccttttttac caactcttcc
      481 ggtacgccct attctatgaa tatagtcatt gtagtttacc ggctcatcaa agtttatgac
      541 gtggcttata tcgggaacat caattcctct ggcggcgaca tcagttgcta cgagaacatc
      601 tattttatcg gttctaaaat ttgtgagtac ttttgttctg actctttggg acttattgcc
      661 gtgaagggaa tctaccataa cgcccctgct tcttagttct tgggttatcc tatctgcacc
      721 tattcttgtt ccggaaaata ccaatacttt agtgaactct gtttttttga gaaggtcgtg
      781 taatatttga attttatgct ctttagaagc aaccttcaca atatcctggt ggatactatt
      841 aaggggagac tgtttgtcgg cctcaacttt tacgggatct ctcaacaatg tatttgcaag
      901 ggcttcttct tttcctgcca ttgttgcgga aaagaacaaa gattgcctat tttccggcaa
      961 tttaccgcaa actattttaa tatcttctac aaatcccatg tctaacattt gatcaacttc
     1021 gtctaatatt atatttgtaa aaccttttaa acttagctga tttctttttt caagatccat
     1081 aagccttccg ggagttgcaa ctacaaactg tggcttctca cgaagagcgc gcatttgaaa
     1141 gtgcatgctc tcgccgccta taacaaggac catccttaat tttgtatcac gtgtaaattt
     1201 tctaaactct acctgaattt gacttgcgag ctctcttgtg ggagttatta ttagaaccct
     1261 attttcgggg ttctttaata ctttctggca cataggaatt aaaaatgcac cagtctttcc
     1321 ggttcctgta ttgcctgttc ccaaaacgtc tcttcctttt agaatgtggg gaatagacat
     1381 atcctgtatt tttgttggtt gaatgtatcc tcggtcagag atatttttca ataatgtagg
     1441 gtgcaaatta aaatctctaa atgaattctc aattacacag gcctcaacta attgagcctg
     1501 cacgggttta gatatgtatc tggaatgatc aataacctcg cttgattttc ttctgttttt
     1561 tcttttccaa ttatcccttg aacgaccttg cttgctaaat tgctgctctc ctgatctatt
     1621 tatataaccg aacgagtttc ctgacctttg gccgcccctt gactctccat cagatccaga
     1681 gcttgttgaa aacttactct tacctttata accaccatta cgacgaccga accctccggg
     1741 cctagctccg cttggcgcac ttgaatagcg tctattttta gactgttttt gtgtat
//
LOCUS       DAOSWN010000087         1787 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_56402_length_1787_cov_4.378176, whole genome shotgun sequence.
ACCESSION   DAOSWN010000087 DAOSWN010000000
VERSION     DAOSWN010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56402_length_1787_cov_4.378176"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..1092)
                     /gene="pilM"
                     /locus_tag="PLF29_03550"
     CDS             complement(40..1092)
                     /gene="pilM"
                     /locus_tag="PLF29_03550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HXK53037.1"
                     /translation="MPIIGLDLGRHQFRAVELEQQKGFYILKNYGNYEGRMLDLDFRS
                     PDAVANYTNALKEFIRERDFSTSGVIVALPEQEVFIRVISTPKLSQKELNNFIKMNAE
                     EYIPIPMSEVTFDAQIIDSNEVVDKEDSETGKTMSALLVASKNDILKKYVELIKSAGF
                     VPKGIEPETLSIERVLGDTLDRPSACIIVNIGVFSTQIIVSYRGFVRFTRTVSVGGSD
                     LTKAVQKALNLEFNQAEEYKKAYGLDESQVDGKVYNAIKPVFDNILLEINRSKMYYTT
                     HNPSVIINRVILSGGTALMPGTLLYIANKLDVEAELSNPWRTIKISEKLQKDRDNLIK
                     QGPMYSTAVGLALKEV"
     gene            complement(1136..>1787)
                     /locus_tag="PLF29_03555"
     CDS             complement(1136..>1787)
                     /locus_tag="PLF29_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK53038.1"
                     /translation="AGIENILLKTNDELYDYIQYNSCGDSCIVSWTNADGLVETATIT
                     LSTLGSSSDAYIFQISQNEVEEINLNGYPEDTSLTLCWDDDFSEYTSVYAELLSGTVG
                     SYTLTPLAYNSINYGGNSNGFDSASSQLGYDSCFDVNSGSDPLSLRIRIFGSDATIAI
                     IPSSSTDLPAQGVLIESVGEVAGTYKKVAVIRKDAFMGPEFSYVLFSTSENSPLSN"
BASE COUNT          513 a          365 c          342 g          567 t
ORIGIN      
        1 cttcttgtgg aattagattt atactttgtg acatggcagt tacacctcct ttagggctag
       61 tccaaccgct gttgaataca ttggcccttg tttaattagg ttatcgcgat ctttttgtaa
      121 tttttcggaa atttttatag ttctccaggg attggaaagc tcggcttcaa catcaagttt
      181 atttgcaata taaagcagtg tacccggcat aagggcagtt cctccggaaa gtattactct
      241 gttaatgata acagaaggat tgtgcgttgt gtagtacatt ttagatctgt ttatttcaag
      301 tagaatattg tcaaatacgg gcttaatagc attgtaaacc ttgccgtcaa cctggctctc
      361 gtctaaaccg taagcttttt tatattcttc tgcctgattg aattctaaat tcagagcttt
      421 ctgaacagct ttagtcaggt cgcttccgcc taccgacacg gttcgagtga agcgaacaaa
      481 acctctataa ctaactatta tttgagtaga aaaaactcca atattcacaa taatgcacgc
      541 gcttggacga tccaaggtat cccctaatac tctctctatc gacaaggttt ctggttctat
      601 gcctttgggg acgaatcctg cggattttat aagctcgacg tattttttta ggatatcgtt
      661 ttttgaagcc actaacaagg cgctcattgt tttaccggtt tcggaatctt ctttatccac
      721 aacttcgtta gagtctatta tttgagcgtc aaaagttacc tcagacatcg ggataggaat
      781 gtattcctca gcattcattt ttataaaatt atttaactct ttttgtgaaa gcttgggagt
      841 actaatgacc cgtataaaga cctcttgctc aggcaatgct acgataactc ctgatgttga
      901 aaaatctctc tctcgaatga actctttgag tgcgttggta tagtttgcga cagcgtcagg
      961 agaacgaaaa tccaaatcga gcatcctgcc ttcatagtta ccgtagtttt taagaatata
     1021 aaaacctttt tgttgttcta attctaccgc cctaaattga tggcggccca gatcaagtcc
     1081 tataatgggc ataatataag tctacagaat atttaaaaaa tattctactc ctaaatcaat
     1141 tagacaacgg gctgttttca gaagttgaaa aaagcacata gctaaattcc gggcccatga
     1201 aagcgtcttt tcttattact gcaactttct tataagttcc tgctacctca ccgaccgatt
     1261 caattagcac tccctgggca ggaaggtctg tagaagaaga gggtattatt gcgatggttg
     1321 catctgaacc aaaaatacga attcttaatg ataaaggatc ggagcctgaa tttacatcaa
     1381 aacagctatc ataccctaac tggctcgagg cagagtcaaa cccattgctg tttccgccat
     1441 aatttatgga gttataagca aggggtgtaa gagtataact accaacagtg ccggacagca
     1501 gttcagcata gacactagtg tactcagaaa aatcatcatc ccagcaaaga gtcagcgaag
     1561 tatcctcggg gtaaccgttt aaatttatct cctctacttc gttctgagat atttgaaaaa
     1621 tataagcatc agaagaacta cctagagtgc ttagcgttat tgtagctgtt tcaactagtc
     1681 cgtcggcgtt tgtccaagag acaatacagc tgtccccgca gctgttgtat tgaatatagt
     1741 cgtaaagctc gtcatttgtt tttaataaaa tgttctctat tccggct
//
LOCUS       DAOSWN010000088         1723 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_59900_length_1723_cov_5.944844, whole genome shotgun sequence.
ACCESSION   DAOSWN010000088 DAOSWN010000000
VERSION     DAOSWN010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59900_length_1723_cov_5.944844"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PLF29_03560"
     CDS             complement(<1..500)
                     /locus_tag="PLF29_03560"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HXK53039.1"
                     /translation="MTEAAQTELNRRKTDHEIDLIEIITILWKARKFIAIVTAISLAI
                     GILYIIFATKMYSGTITLYPAQSGAKNPMVQMAAQMGMASASAGDANYNIPDVVKSRT
                     LSEQIVAHKWEIEGFDKPIDLVEYFDTITGKLDLSNSEKEKYGLYKYSEMVSNDRISV
                     DSNTKTG"
     gene            complement(578..>1723)
                     /locus_tag="PLF29_03565"
     CDS             complement(578..>1723)
                     /locus_tag="PLF29_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006460947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK53040.1"
                     /translation="EGKHIIEVSVNKTFAPVSFEGKFYKRSGSNTIELNGSNLTNFLL
                     KKYGKTWDDIAVEEFKLSEIDLESISKFKKLARDRVPDIEREDNLKELLKKLNLYDGK
                     YLKRAAVLLFAKDPQKYFIQSHSKIGKFLSETEILTSDIIEGNLIDQVDKIMDILRIK
                     YLKAYISYEGIHRREKLEYPYSALKEAVINALIHRDYSNTSNLQIKVYDDKLIMYNGA
                     LLSSEVPIEKFTKPHQSRPFNPIIASVFYKAGLIENWGKGTLNIIRDCKEQGLPSPVY
                     RYEWTAVSVTFYKALVNEPKEVTEKVTKEVTKEVTKEVTKEVTKEQALILEKMEDNIK
                     VTISELSEELELSPKTIKKYITRLTDSGLLKREGGRKLGRWVVLKRK"
BASE COUNT          401 a          357 c          308 g          657 t
ORIGIN      
        1 cctgttttag tattcgaatc tacacttatt ctatcatttg ataccatttc actgtacttg
       61 taaagtccat atttctcttt ttcactattg cttaaatcta acttgccggt aatggtgtca
      121 aaatattcta cgagatctat cggtttatca aatccttcta tttcccattt atgagctacg
      181 atctgttcgg aaagtgttct gcttttgact acatcgggga tgttatagtt cgcgtcgcct
      241 gctgacgcac tggccattcc catttgtgct gccatttgta ccatggggtt ttttgcgcct
      301 gattgtgcgg gatagagtgt tattgttccg ctgtacattt tggtggcgaa gattatgtat
      361 agtattccta ttgcgaggga tattgcggtg actatggcta tgaattttct ggctttccag
      421 aggatggtga ttatttctat aaggtctatt tcgtggtcgg ttttccggcg gtttagttct
      481 gtctgagcgg cttctgtcat ttgtgttccc tgtattattt gaatttcttt tttatacgct
      541 gtaaatataa taaagttttg cggtaaatca aagagggtta ttttcgctta agcactaccc
      601 agcggccgag cttccttcct ccttctcgct taagtagacc ggaatctgta agtctggtta
      661 tgtatttctt tatagttttt ggcgacaatt ctaattcttc tgataattca gaaatcgtta
      721 ccttaatgtt gtcttccatt ttctctaaaa ttaatgcctg ttcttttgtc acctcttttg
      781 tcacctcttt tgtcacctct tttgtcacct cttttgtcac cttttctgtc acctcttttg
      841 gctcattaac taaggcttta taaaaagtta cgcttacagc tgtccactca tacctgtaaa
      901 ccggactggg aagaccctgc tctttgcagt ctctgattat gtttaaggta cccttacccc
      961 agttctcgat caatcctgct ttgtagaata cggatgcaat tatcggattg aaaggtcttg
     1021 actggtgagg cttagtgaac ttctcaatag gaacttcgct gcttaataaa gcaccgttat
     1081 acattatcag cttatcgtcg taaactttga tctgcaggtt tgaagtattt gaataatctc
     1141 tatgaatcaa agcattaata acagcctctt ttaaagcact gtaaggatat tctaatttct
     1201 cccttctgtg aataccctca taagaaatat aggctttaag gtattttatc ctcaagatat
     1261 ccattatttt atcaacctgg tcaatcagat tcccttctat tatatcgctt gtcagaatct
     1321 cagtctctga taaaaacttt cctatctttg agtgagactg tataaaatat ttctgaggat
     1381 cttttgcaaa caggagaact gccgctcttt tcaaatattt accgtcataa agattaagct
     1441 ttttaagcag ttccttcaga ttgtcttccc tttcgatgtc cgggacacgg tctcttgcaa
     1501 gttttttgaa tttgctgata ctctctaagt ctatctcgct gagcttgaac tcttctaccg
     1561 caatatcatc ccatgttttt ccgtatttct ttaaaagaaa gttggtcagg ttgctgccgt
     1621 taagctcgat cgtattgctg ccgctccttt tatagaattt tccctcgaat gataccggag
     1681 cgaatgtttt attgacagaa acttcgatta tatgttttcc ttc
//
LOCUS       DAOSWN010000089         1659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_63866_length_1659_cov_3.496883, whole genome shotgun sequence.
ACCESSION   DAOSWN010000089 DAOSWN010000000
VERSION     DAOSWN010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63866_length_1659_cov_3.496883"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PLF29_03570"
     CDS             complement(<1..201)
                     /locus_tag="PLF29_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK53041.1"
                     /translation="MHSRPWKNIKKSRKYMYIDKNKNRWYTYKPRSGEFTRISRGMLD
                     TFLKCPRCFYLQKVLGVKAPHIP"
     gene            complement(357..1145)
                     /locus_tag="PLF29_03575"
     CDS             complement(357..1145)
                     /locus_tag="PLF29_03575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HXK53042.1"
                     /translation="MMNIVSWNINGLKSCLNKGLADFIKTDKSDIYCFQESKISEENF
                     DQLQNCLFPVFYGYNAYNFAASTRKGYSGLVILSKNTPIKVTNGLGIKGFDEEARVQI
                     AEYPSFFLINTYVPHPRADFSRLEYKMSFCESLLKLASELKQKKKVVLAGDFNVAHTN
                     LDLQNPKMNKGSPMFSLIERAWVDKVIENNFFDVFRYLYPEKESYSWFPYSINNIARE
                     KNKGWRIDYFFLDIRFKGSVQELTHLKNISGSDHVPLKIVMGEI"
BASE COUNT          463 a          323 c          302 g          571 t
ORIGIN      
        1 aggtatgtgt ggggctttta ctcccaatac tttctgaagg taaaagcacc tagggcactt
       61 taagaaagta tcgagcatcc ctctgctaat acgcgtgaat tccccgcttc tcggtttata
      121 tgtgtaccat ctgtttttgt ttttatctat atacatgtat tttcgactct tctttatatt
      181 tttccacggc ctagaatgca ttgctgcttc ttgagattta gataagtagg atgacgcatg
      241 tttattgtat caagcatata ttcggccttc aatgttttga gttggtccat tgagttttat
      301 accctatgtt gcatgctgaa cctagttcag tccttatgaa aaacggtggt tagattttaa
      361 atctctccca taacaatctt tagcggaacg tggtctgatc cagaaatatt ttttaaatgc
      421 gtgagttctt gaactgagcc tttaaacctg atgtctaaaa agaagtagtc tattctccac
      481 cctttatttt tttctcttgc gatgttgttg attgagtatg gaaaccagct gtaagattct
      541 ttttccgggt ataaatatct gaaaacgtca aagaaattgt tttcaattac tttgtcgacc
      601 catgctcttt ctatcaagga gaacatggga gagcctttat tcatttttgg attttgaaga
      661 tcaaggtttg tatgcgccac attgaagtcc ccagctaaca ccactttctt tttttgcttt
      721 agttcggacg caagctttaa taaagattcg caaaaactca tcttatactc tagccttgag
      781 aagtctgctc gggggtgagg tacatacgta ttgatcaaga aaaaagacgg gtattcagca
      841 atttgaaccc ttgcttcttc gtcgaaccct tttattccca gcccatttgt tacttttatt
      901 ggagtgtttt tagatagtat aactagacct gagtatcctt ttcgcgtcga agccgcaaaa
      961 ttataggcgt tataaccata aaatacaggg aacaagcagt tttgcagttg gtcgaagttt
     1021 tcttcagata ttttgctttc ttgaaagcaa tatatatctg atttgtcggt ttttataaag
     1081 tcagcaagtc ccttatttaa gcaagacttt aatccgttga tgttccacga tactatgttc
     1141 atcatttaaa tcagatacta ctccaggctt gctgctctta caacaatttt cccattactt
     1201 aaagtcctgc tattactgcc ctctccacgc tgtaggttga atagggtaca catttagtgc
     1261 ttgcataaga aaaaggccta agagagatct agaaagacta cccggtttgc tataacccaa
     1321 gcagcgcctt tagtttatcc gggaaagatt gccctgttgt atcgacattc aaaagaaatt
     1381 ctattgaatc ctggccggat ttaatctcaa tcttatgttt attgccctct aaattaaaag
     1441 acagaacata ttcgttttga tccgctgttg tgctatatct gtctctcaga tgtttacttg
     1501 actggcagta ctgtgcgcta tctgagccta tttccagata ctttctttac taaaactaag
     1561 aacgggaatc cgataatatt acctacaaaa ctagagatta ccgacaagaa taacggaaaa
     1621 atattgtaaa taatactaaa tcgagaaaag gcactggtt
//
LOCUS       DAOSWN010000090         1582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2136
            NODE_69156_length_1582_cov_3.760969, whole genome shotgun sequence.
ACCESSION   DAOSWN010000090 DAOSWN010000000
VERSION     DAOSWN010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864203
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:42:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 717
            CDSs (total)                      :: 685
            Genes (coding)                    :: 683
            CDSs (with protein)               :: 683
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69156_length_1582_cov_3.760969"
                     /isolate="AMR_MDS_2136"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="PLF29_03580"
     CDS             <1..892
                     /locus_tag="PLF29_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK53043.1"
                     /translation="AKRATFTLFMALVVVSIPLFLLLKKYTFEVQIYSVSDKILHRSF
                     SEISDSIYISSIDTSVPSFFSKDREVSVSAQVLVPDSIEITFDVQQKIQSELSEALNS
                     DVNFSLLLQRSLGVTSKKASEVSKKRSLLQAALVSELRMSDANYDIDSIDISYLEDRG
                     VWRVSAVLRADTEAFRPNINSGALENTLSELVNTPVELSIELIPRIAIGMDTANIEKV
                     RSSVMSYLSDNYPQIDVKSAYVEYNEGENRYQISIEVLVPFSFQAGYDVQQLKSYLEE
                     TFNSEFALTETIIYADKIIY"
     gene            870..1364
                     /locus_tag="PLF29_03585"
     CDS             870..1364
                     /locus_tag="PLF29_03585"
                     /inference="COORDINATES: protein motif:HMM:NF019499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HXK53044.1"
                     /translation="MQTKLFIKSLLPAIFILVFVGLVISAMLHIEYSSENTFICGYTA
                     DVSGCETVKNSEYSYLFGIRLPVLGVAFFSTFLLLSGLGVFVQAGYLRTQLFKDFFKG
                     RLYSFSILLLGLVGAFVEVYLLFIQHVILKEYCSWCLLLGIVALVVFVLSNFYFFSRN
                     YFDK"
BASE COUNT          436 a          257 c          326 g          563 t
ORIGIN      
        1 tgctaaaagg gctactttta cactttttat ggctctggtt gttgttagca ttcctttgtt
       61 tctcttgttg aaaaaatata cttttgaagt tcaaatatat tcagtgtcgg ataaaatctt
      121 acatcgaagc ttttcagaaa tatctgattc tatttatata tcaagtatag atactagtgt
      181 tccttccttc ttctcaaaag atagagaggt ttcagtatct gcacaggtat tggtgcccga
      241 cagcatagag ataacattcg atgtacagca gaaaattcag tccgagcttt ccgaagcgtt
      301 aaattccgac gttaatttta gccttcttct gcaaaggtcg ttgggagtaa cgagtaaaaa
      361 agcatctgag gtttcaaaaa aaaggtcatt attgcaggcg gcactagttt ctgagctccg
      421 gatgagcgac gctaactacg acatcgacag catagacatt tcttatttag aagatcgtgg
      481 cgtgtggaga gtaagtgcag ttctgagagc tgacacagaa gcgttccgcc caaacattaa
      541 tagcggggct ttagaaaata ctttatcaga gctcgttaat actcccgttg agctcagcat
      601 tgagttaatt ccaagaattg ctattggtat ggatacagct aacattgaaa aagtcagatc
      661 ctctgtcatg tcctatttgt ctgataatta tcctcaaatt gatgtaaaat cagcgtacgt
      721 tgaatacaat gagggtgaaa ataggtatca gattagtatt gaggtgcttg tacctttttc
      781 ttttcaagcc ggatatgatg tgcaacaatt aaagtcgtac ttagaggaaa catttaatag
      841 tgaattcgcg cttactgaaa caataatata tgcagacaaa attatttatt aaaagcttac
      901 ttcctgcaat ttttatatta gtttttgtcg gtcttgtgat atcagccatg cttcatattg
      961 aatatagcag tgaaaacacc tttatatgcg gttatactgc agatgtttcg gggtgcgaaa
     1021 ccgtaaaaaa tagcgagtac tcatatttat tcggtataag acttcctgtt ttaggtgtag
     1081 ctttcttttc tacattttta ttattatcgg gtttgggtgt atttgtacaa gcgggctatc
     1141 taagaacgca gttattcaag gatttcttta aaggaaggct ctatagcttt tctatcctac
     1201 tcttaggctt agttggagct tttgttgaag tttatctact ttttattcag cacgtaatcc
     1261 ttaaggaata ttgttcgtgg tgcttgttgt tgggtatagt cgctctcgtt gtatttgttc
     1321 tttcgaattt ttactttttt tctagaaatt attttgataa atagccgcat acatctttaa
     1381 ttgcgtcttt ttacagttat tttgactctt aggctttatt ttttctgttt ttataatttc
     1441 gttacttaaa actacttgcg tgagctagta ggtatagttt acccaacatg ttgggctttg
     1501 cagtgcaagg aattacagca aaccatctgt gtaagctggc ggtggtagat taaagtatgc
     1561 gtgtgtgggt atgggggggg cg
//
